LOCUS       DACGLW010000001       123572 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.001, whole genome shotgun sequence.
ACCESSION   DACGLW010000001 DACGLW010000000
VERSION     DACGLW010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 123572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 123572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..123572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.001"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            complement(41..1249)
                     /locus_tag="IXT53_000001"
     CDS             complement(41..1249)
                     /locus_tag="IXT53_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ4903870.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(1268..1546)
                     /locus_tag="IXT53_000002"
     CDS             complement(1268..1546)
                     /locus_tag="IXT53_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4903871.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            1739..2395
                     /locus_tag="IXT53_000003"
     CDS             1739..2395
                     /locus_tag="IXT53_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ4903872.1"
                     /translation="MDSRVLKEYLDKNDFPIIRKGYHKYLTFEGMEDSHTYILKEGTV
                     KASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESEYAEFYRINRVRFW
                     QNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLYELYDLFGVRTEEG
                     MLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIVILNMDLLEDNIIF
                     "
     gene            complement(2837..3634)
                     /locus_tag="IXT53_000004"
     CDS             complement(2837..3634)
                     /locus_tag="IXT53_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HAQ4903873.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(3631..4815)
                     /locus_tag="IXT53_000005"
     CDS             complement(3631..4815)
                     /locus_tag="IXT53_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAQ4903874.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(4840..6087)
                     /locus_tag="IXT53_000006"
     CDS             complement(4840..6087)
                     /locus_tag="IXT53_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virion core protein"
                     /protein_id="HAQ4903875.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            6302..8047
                     /gene="dnaX"
                     /locus_tag="IXT53_000007"
     CDS             6302..8047
                     /gene="dnaX"
                     /locus_tag="IXT53_000007"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HAQ4903876.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            8067..8381
                     /locus_tag="IXT53_000008"
     CDS             8067..8381
                     /locus_tag="IXT53_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HAQ4903877.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            8406..9002
                     /gene="recR"
                     /locus_tag="IXT53_000009"
     CDS             8406..9002
                     /gene="recR"
                     /locus_tag="IXT53_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HAQ4903878.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            9066..9710
                     /locus_tag="IXT53_000010"
     CDS             9066..9710
                     /locus_tag="IXT53_000010"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HAQ4903879.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            9725..10054
                     /locus_tag="IXT53_000011"
     CDS             9725..10054
                     /locus_tag="IXT53_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4903880.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            10068..11006
                     /gene="holB"
                     /locus_tag="IXT53_000012"
     CDS             10068..11006
                     /gene="holB"
                     /locus_tag="IXT53_000012"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HAQ4903881.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            11042..11866
                     /locus_tag="IXT53_000013"
     CDS             11042..11866
                     /locus_tag="IXT53_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HAQ4903882.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            11859..12206
                     /locus_tag="IXT53_000014"
     CDS             11859..12206
                     /locus_tag="IXT53_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HAQ4903883.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            12275..13147
                     /gene="rsmI"
                     /locus_tag="IXT53_000015"
     CDS             12275..13147
                     /gene="rsmI"
                     /locus_tag="IXT53_000015"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HAQ4903884.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            13256..14377
                     /gene="dinB"
                     /locus_tag="IXT53_000016"
     CDS             13256..14377
                     /gene="dinB"
                     /locus_tag="IXT53_000016"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HAQ4903885.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(14431..15033)
                     /gene="nrdG"
                     /locus_tag="IXT53_000017"
     CDS             complement(14431..15033)
                     /gene="nrdG"
                     /locus_tag="IXT53_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HAQ4903886.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(15348..17537)
                     /gene="nrdD"
                     /locus_tag="IXT53_000018"
     CDS             complement(15348..17537)
                     /gene="nrdD"
                     /locus_tag="IXT53_000018"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HAQ4903887.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            17863..18630
                     /locus_tag="IXT53_000019"
     CDS             17863..18630
                     /locus_tag="IXT53_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4903888.1"
                     /translation="MEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGEFVGIMGPSG
                     AGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQEFNLIDTLN
                     AKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQRIAAARALV
                     VQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTASYCDRILFIK
                     DGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            18620..20662
                     /locus_tag="IXT53_000020"
     CDS             18620..20662
                     /locus_tag="IXT53_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ4903889.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            20943..21092
                     /locus_tag="IXT53_000021"
     CDS             20943..21092
                     /locus_tag="IXT53_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HAQ4903890.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            21103..22617
                     /gene="dltA"
                     /locus_tag="IXT53_000022"
     CDS             21103..22617
                     /gene="dltA"
                     /locus_tag="IXT53_000022"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HAQ4903891.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            22614..23819
                     /gene="dltB"
                     /locus_tag="IXT53_000023"
     CDS             22614..23819
                     /gene="dltB"
                     /locus_tag="IXT53_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HAQ4903892.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            23864..24097
                     /gene="dltC"
                     /locus_tag="IXT53_000024"
     CDS             23864..24097
                     /gene="dltC"
                     /locus_tag="IXT53_000024"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HAQ4903893.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            24100..25368
                     /gene="dltD"
                     /locus_tag="IXT53_000025"
     CDS             24100..25368
                     /gene="dltD"
                     /locus_tag="IXT53_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HAQ4903894.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            25509..26591
                     /locus_tag="IXT53_000026"
     CDS             25509..26591
                     /locus_tag="IXT53_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HAQ4903895.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            26591..27394
                     /locus_tag="IXT53_000027"
     CDS             26591..27394
                     /locus_tag="IXT53_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAQ4903896.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPMGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            27464..28204
                     /locus_tag="IXT53_000028"
     CDS             27464..28204
                     /locus_tag="IXT53_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ4903897.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            28215..29219
                     /locus_tag="IXT53_000029"
     CDS             28215..29219
                     /locus_tag="IXT53_000029"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HAQ4903898.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(29264..31108)
                     /locus_tag="IXT53_000030"
     CDS             complement(29264..31108)
                     /locus_tag="IXT53_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4903899.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
     gene            31320..31837
                     /locus_tag="IXT53_000031"
                     /pseudo
     CDS             31320..31837
                     /locus_tag="IXT53_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
     gene            31965..32648
                     /locus_tag="IXT53_000032"
     CDS             31965..32648
                     /locus_tag="IXT53_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HAQ4903900.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            32957..33030
                     /locus_tag="IXT53_000033"
     tRNA            32957..33030
                     /locus_tag="IXT53_000033"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32991..32993,aa:Arg,seq:tct)
     gene            33189..33548
                     /locus_tag="IXT53_000034"
     CDS             33189..33548
                     /locus_tag="IXT53_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HAQ4903901.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            33671..35302
                     /locus_tag="IXT53_000035"
     CDS             33671..35302
                     /locus_tag="IXT53_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HAQ4903902.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(35364..36005)
                     /locus_tag="IXT53_000036"
     CDS             complement(35364..36005)
                     /locus_tag="IXT53_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAQ4903903.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     gene            36287..36595
                     /gene="rplU"
                     /locus_tag="IXT53_000037"
     CDS             36287..36595
                     /gene="rplU"
                     /locus_tag="IXT53_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HAQ4903904.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            36622..36966
                     /locus_tag="IXT53_000038"
     CDS             36622..36966
                     /locus_tag="IXT53_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HAQ4903905.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            36979..37272
                     /gene="rpmA"
                     /locus_tag="IXT53_000039"
     CDS             36979..37272
                     /gene="rpmA"
                     /locus_tag="IXT53_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HAQ4903906.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            37679..40513
                     /locus_tag="IXT53_000040"
     CDS             37679..40513
                     /locus_tag="IXT53_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ4903907.1"
                     /translation="MAKRIDRIYMYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNS
                     SKDLNQLVREGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDT
                     KDIFAKIIGANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAH
                     HVIDEETELIVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLD
                     EIHRLPPEGQEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRI
                     PINIKLPAFEKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQL
                     KSNIQLVCARGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKIT
                     VAPNDTIMKIQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFY
                     KDHGFSFNADSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKK
                     INLGEERHTNDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLR
                     ANQASGRIGVVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNV
                     QEVDEGSGVILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDL
                     DMLHESLKNFRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNK
                     RQETELEVLALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVI
                     LDQLLMESELEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTT
                     GIGDEEYGFKINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLED
                     QIKVKVPIEEMYYLLRLVHNQLDKKEYTVP"
     gene            40704..41195
                     /locus_tag="IXT53_000041"
     CDS             40704..41195
                     /locus_tag="IXT53_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIB"
                     /protein_id="HAQ4903908.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            41298..42287
                     /gene="manX"
                     /locus_tag="IXT53_000042"
     CDS             41298..42287
                     /gene="manX"
                     /locus_tag="IXT53_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HAQ4903909.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            42320..43123
                     /locus_tag="IXT53_000043"
     CDS             42320..43123
                     /locus_tag="IXT53_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HAQ4903910.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            43144..44058
                     /locus_tag="IXT53_000044"
     CDS             43144..44058
                     /locus_tag="IXT53_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HAQ4903911.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            44141..44515
                     /locus_tag="IXT53_000045"
     CDS             44141..44515
                     /locus_tag="IXT53_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HAQ4903912.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            44654..44899
                     /locus_tag="IXT53_000046"
     CDS             44654..44899
                     /locus_tag="IXT53_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ4903913.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            45071..46135
                     /locus_tag="IXT53_000047"
     CDS             45071..46135
                     /locus_tag="IXT53_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAQ4903914.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            46381..46806
                     /locus_tag="IXT53_000048"
     CDS             46381..46806
                     /locus_tag="IXT53_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ4903915.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            46799..47248
                     /gene="nusB"
                     /locus_tag="IXT53_000049"
     CDS             46799..47248
                     /gene="nusB"
                     /locus_tag="IXT53_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HAQ4903916.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            47345..48199
                     /locus_tag="IXT53_000050"
     CDS             47345..48199
                     /locus_tag="IXT53_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HAQ4903917.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            48200..49537
                     /locus_tag="IXT53_000051"
     CDS             48200..49537
                     /locus_tag="IXT53_000051"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HAQ4903918.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            49542..49766
                     /locus_tag="IXT53_000052"
     CDS             49542..49766
                     /locus_tag="IXT53_000052"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HAQ4903919.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            49768..50658
                     /locus_tag="IXT53_000053"
     CDS             49768..50658
                     /locus_tag="IXT53_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAQ4903920.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            50667..51485
                     /locus_tag="IXT53_000054"
     CDS             50667..51485
                     /locus_tag="IXT53_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HAQ4903921.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            51592..52041
                     /locus_tag="IXT53_000055"
     CDS             51592..52041
                     /locus_tag="IXT53_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAQ4903922.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            52059..53741
                     /gene="recN"
                     /locus_tag="IXT53_000056"
     CDS             52059..53741
                     /gene="recN"
                     /locus_tag="IXT53_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HAQ4903923.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(53796..53912)
                     /locus_tag="IXT53_000057"
     CDS             complement(53796..53912)
                     /locus_tag="IXT53_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HAQ4903924.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(53985..54941)
                     /locus_tag="IXT53_000058"
     CDS             complement(53985..54941)
                     /locus_tag="IXT53_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ4903925.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            55112..55480
                     /locus_tag="IXT53_000059"
     CDS             55112..55480
                     /locus_tag="IXT53_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HAQ4903926.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            55650..56081
                     /gene="mraZ"
                     /locus_tag="IXT53_000060"
     CDS             55650..56081
                     /gene="mraZ"
                     /locus_tag="IXT53_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HAQ4903927.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            56102..57061
                     /gene="rsmH"
                     /locus_tag="IXT53_000061"
     CDS             56102..57061
                     /gene="rsmH"
                     /locus_tag="IXT53_000061"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HAQ4903928.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            57066..57467
                     /gene="ftsL"
                     /locus_tag="IXT53_000062"
     CDS             57066..57467
                     /gene="ftsL"
                     /locus_tag="IXT53_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HAQ4903929.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            57468..59660
                     /locus_tag="IXT53_000063"
     CDS             57468..59660
                     /locus_tag="IXT53_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAQ4903930.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKTGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            59686..60648
                     /locus_tag="IXT53_000064"
     CDS             59686..60648
                     /locus_tag="IXT53_000064"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HAQ4903931.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            60662..62023
                     /locus_tag="IXT53_000065"
     CDS             60662..62023
                     /locus_tag="IXT53_000065"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HAQ4903932.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            62049..63137
                     /gene="murG"
                     /locus_tag="IXT53_000066"
     CDS             62049..63137
                     /gene="murG"
                     /locus_tag="IXT53_000066"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HAQ4903933.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            63152..64372
                     /locus_tag="IXT53_000067"
     CDS             63152..64372
                     /locus_tag="IXT53_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HAQ4903934.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            64528..65853
                     /gene="ftsA"
                     /locus_tag="IXT53_000068"
     CDS             64528..65853
                     /gene="ftsA"
                     /locus_tag="IXT53_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HAQ4903935.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            65876..67117
                     /gene="ftsZ"
                     /locus_tag="IXT53_000069"
     CDS             65876..67117
                     /gene="ftsZ"
                     /locus_tag="IXT53_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HAQ4903936.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            67134..67811
                     /locus_tag="IXT53_000070"
     CDS             67134..67811
                     /locus_tag="IXT53_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HAQ4903937.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            67825..68424
                     /locus_tag="IXT53_000071"
     CDS             67825..68424
                     /locus_tag="IXT53_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HAQ4903938.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            68439..68726
                     /locus_tag="IXT53_000072"
     CDS             68439..68726
                     /locus_tag="IXT53_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HAQ4903939.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            68775..69557
                     /locus_tag="IXT53_000073"
     CDS             68775..69557
                     /locus_tag="IXT53_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HAQ4903940.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            69578..70285
                     /locus_tag="IXT53_000074"
     CDS             69578..70285
                     /locus_tag="IXT53_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HAQ4903941.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            70547..73345
                     /gene="ileS"
                     /locus_tag="IXT53_000075"
     CDS             70547..73345
                     /gene="ileS"
                     /locus_tag="IXT53_000075"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HAQ4903942.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(73394..74920)
                     /locus_tag="IXT53_000076"
     CDS             complement(73394..74920)
                     /locus_tag="IXT53_000076"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HAQ4903943.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(74935..75582)
                     /locus_tag="IXT53_000077"
     CDS             complement(74935..75582)
                     /locus_tag="IXT53_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HAQ4903944.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(75766..76095)
                     /locus_tag="IXT53_000078"
     CDS             complement(75766..76095)
                     /locus_tag="IXT53_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HAQ4903945.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            76272..77000
                     /gene="sfsA"
                     /locus_tag="IXT53_000079"
     CDS             76272..77000
                     /gene="sfsA"
                     /locus_tag="IXT53_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HAQ4903946.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            77016..78029
                     /locus_tag="IXT53_000080"
     CDS             77016..78029
                     /locus_tag="IXT53_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ4903947.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            78029..79306
                     /locus_tag="IXT53_000081"
     CDS             78029..79306
                     /locus_tag="IXT53_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ4903948.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            79369..82071
                     /locus_tag="IXT53_000082"
     CDS             79369..82071
                     /locus_tag="IXT53_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ4903949.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            82223..82540
                     /locus_tag="IXT53_000083"
     CDS             82223..82540
                     /locus_tag="IXT53_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ4903950.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            82570..82890
                     /locus_tag="IXT53_000084"
     CDS             82570..82890
                     /locus_tag="IXT53_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ4903951.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            82998..84458
                     /locus_tag="IXT53_000085"
     CDS             82998..84458
                     /locus_tag="IXT53_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ4903952.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            84526..84747
                     /locus_tag="IXT53_000086"
     CDS             84526..84747
                     /locus_tag="IXT53_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4903953.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            84778..84960
                     /locus_tag="IXT53_000087"
     CDS             84778..84960
                     /locus_tag="IXT53_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HAQ4903954.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            84960..85373
                     /locus_tag="IXT53_000088"
     CDS             84960..85373
                     /locus_tag="IXT53_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HAQ4903955.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            85496..86677
                     /locus_tag="IXT53_000089"
     CDS             85496..86677
                     /locus_tag="IXT53_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ4903956.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            87066..87139
                     /locus_tag="IXT53_000090"
     tRNA            87066..87139
                     /locus_tag="IXT53_000090"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:87100..87102,aa:Arg,seq:cct)
     gene            complement(87208..88347)
                     /locus_tag="IXT53_000091"
     CDS             complement(87208..88347)
                     /locus_tag="IXT53_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ4903957.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(88646..89281)
                     /locus_tag="IXT53_000092"
     CDS             complement(88646..89281)
                     /locus_tag="IXT53_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4903958.1"
                     /translation="MDNSNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(89394..90029)
                     /locus_tag="IXT53_000093"
     CDS             complement(89394..90029)
                     /locus_tag="IXT53_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HAQ4903959.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQFEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(90063..90524)
                     /locus_tag="IXT53_000094"
                     /pseudo
     CDS             complement(90063..90524)
                     /locus_tag="IXT53_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4429 domain-containing protein"
     gene            complement(90654..91085)
                     /locus_tag="IXT53_000095"
     CDS             complement(90654..91085)
                     /locus_tag="IXT53_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HAQ4903960.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(91103..91423)
                     /locus_tag="IXT53_000096"
     CDS             complement(91103..91423)
                     /locus_tag="IXT53_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ4903961.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            91722..92498
                     /locus_tag="IXT53_000097"
     CDS             91722..92498
                     /locus_tag="IXT53_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HAQ4903962.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            92513..92716
                     /locus_tag="IXT53_000098"
     CDS             92513..92716
                     /locus_tag="IXT53_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ4903963.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            92732..93070
                     /locus_tag="IXT53_000099"
     CDS             92732..93070
                     /locus_tag="IXT53_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HAQ4903964.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
     gene            93057..93236
                     /locus_tag="IXT53_000100"
     CDS             93057..93236
                     /locus_tag="IXT53_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4903965.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(93279..93749)
                     /locus_tag="IXT53_000101"
     CDS             complement(93279..93749)
                     /locus_tag="IXT53_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4903966.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            93836..94534
                     /locus_tag="IXT53_000102"
     CDS             93836..94534
                     /locus_tag="IXT53_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HAQ4903967.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            94712..95053
                     /locus_tag="IXT53_000103"
     CDS             94712..95053
                     /locus_tag="IXT53_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4903968.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            95046..95717
                     /locus_tag="IXT53_000104"
     CDS             95046..95717
                     /locus_tag="IXT53_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HAQ4903969.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            95723..96409
                     /locus_tag="IXT53_000105"
     CDS             95723..96409
                     /locus_tag="IXT53_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4903970.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGG"
     gene            96412..97161
                     /locus_tag="IXT53_000106"
     CDS             96412..97161
                     /locus_tag="IXT53_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ4903971.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            97173..97442
                     /locus_tag="IXT53_000107"
     CDS             97173..97442
                     /locus_tag="IXT53_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4903972.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            97604..97906
                     /locus_tag="IXT53_000108"
     CDS             97604..97906
                     /locus_tag="IXT53_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4903973.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            97903..98064
                     /locus_tag="IXT53_000109"
     CDS             97903..98064
                     /locus_tag="IXT53_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAQ4903974.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            98061..98366
                     /locus_tag="IXT53_000110"
     CDS             98061..98366
                     /locus_tag="IXT53_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4903975.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            98366..98722
                     /locus_tag="IXT53_000111"
     CDS             98366..98722
                     /locus_tag="IXT53_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HAQ4903976.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            98682..98927
                     /locus_tag="IXT53_000112"
     CDS             98682..98927
                     /locus_tag="IXT53_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4903977.1"
                     /translation="MIKKLVQFSMDLYDIESGATLSVESDHLIINFGGKRQIILWVVD
                     DVLFPEIVHDFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            98924..99343
                     /locus_tag="IXT53_000113"
     CDS             98924..99343
                     /locus_tag="IXT53_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HAQ4903978.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            99340..99897
                     /locus_tag="IXT53_000114"
     CDS             99340..99897
                     /locus_tag="IXT53_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HAQ4903979.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            99894..100190
                     /locus_tag="IXT53_000115"
     CDS             99894..100190
                     /locus_tag="IXT53_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4903980.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            100267..100680
                     /locus_tag="IXT53_000116"
     CDS             100267..100680
                     /locus_tag="IXT53_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HAQ4903981.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIETERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGKIIAYK"
     gene            complement(101138..101413)
                     /locus_tag="IXT53_000117"
     CDS             complement(101138..101413)
                     /locus_tag="IXT53_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4903982.1"
                     /translation="MYKPYMISYDLNNPGQKYDKVFEIIKEFGAYIKLQKSFWLVKTN
                     LNPNQMCEKLNTVLDNNDSLFICELQKNYQGRATEENWKFINEHIFS"
     gene            101867..102073
                     /locus_tag="IXT53_000118"
     CDS             101867..102073
                     /locus_tag="IXT53_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4903983.1"
                     /translation="MLGQCFGNHARFDSLPATLNAYDGYDYRKKIVKKLYGATYGNVV
                     SVLSVHHQASVTVAEVEDAAVNGE"
     gene            102269..102436
                     /locus_tag="IXT53_000119"
     CDS             102269..102436
                     /locus_tag="IXT53_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAQ4903984.1"
                     /translation="MYRPQYLEQKYEVITVHKGNGEKVYEYRRPIKSDTYKRKENNEV
                     IPLYGKRIAKH"
     gene            102462..102806
                     /locus_tag="IXT53_000120"
     CDS             102462..102806
                     /locus_tag="IXT53_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAQ4903985.1"
                     /translation="MTEEFYRWLLQLTREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            102811..103092
                     /locus_tag="IXT53_000121"
     CDS             102811..103092
                     /locus_tag="IXT53_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4903986.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            103195..103509
                     /locus_tag="IXT53_000122"
     CDS             103195..103509
                     /locus_tag="IXT53_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HAQ4903987.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            103487..105181
                     /locus_tag="IXT53_000123"
     CDS             103487..105181
                     /locus_tag="IXT53_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAQ4903988.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            105201..106379
                     /locus_tag="IXT53_000124"
     CDS             105201..106379
                     /locus_tag="IXT53_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAQ4903989.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            106342..107028
                     /locus_tag="IXT53_000125"
     CDS             106342..107028
                     /locus_tag="IXT53_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HAQ4903990.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            107028..108188
                     /locus_tag="IXT53_000126"
     CDS             107028..108188
                     /locus_tag="IXT53_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAQ4903991.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            108198..109073
                     /locus_tag="IXT53_000127"
     CDS             108198..109073
                     /locus_tag="IXT53_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4903992.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            109070..109381
                     /locus_tag="IXT53_000128"
     CDS             109070..109381
                     /locus_tag="IXT53_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4903993.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            109371..109724
                     /locus_tag="IXT53_000129"
     CDS             109371..109724
                     /locus_tag="IXT53_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAQ4903994.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            109714..110115
                     /locus_tag="IXT53_000130"
     CDS             109714..110115
                     /locus_tag="IXT53_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4903995.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            110108..110512
                     /locus_tag="IXT53_000131"
     CDS             110108..110512
                     /locus_tag="IXT53_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4903996.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            110524..111132
                     /locus_tag="IXT53_000132"
     CDS             110524..111132
                     /locus_tag="IXT53_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ4903997.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            111152..111514
                     /locus_tag="IXT53_000133"
     CDS             111152..111514
                     /locus_tag="IXT53_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4903998.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            111517..111699
                     /locus_tag="IXT53_000134"
     CDS             111517..111699
                     /locus_tag="IXT53_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4903999.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            111716..115147
                     /locus_tag="IXT53_000135"
                     /pseudo
     CDS             111716..115147
                     /locus_tag="IXT53_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
     gene            115198..115935
                     /locus_tag="IXT53_000136"
     CDS             115198..115935
                     /locus_tag="IXT53_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HAQ4904000.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            115945..118236
                     /locus_tag="IXT53_000137"
     CDS             115945..118236
                     /locus_tag="IXT53_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4904001.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYSINGTNADLIVDGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKSLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLLNDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDNGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            118260..120386
                     /locus_tag="IXT53_000138"
     CDS             118260..120386
                     /locus_tag="IXT53_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4904002.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDNVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAEIKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGDYDYSENPNLMPYITEPWVG
                     PLLGNGHTVKDSVKRVITHTKTRTANSGDILSLGLGIPCTAEANNRYLITTLRPSTTY
                     TLSVTMSVGSDWTGETNTIGVRLRYLNEQGGIELPINALIPANVERDKMVTHTFTGIT
                     KDNVTSITNCYVEIFSLNSEYKGTVSVSYDVKLKAHYPNLLDGPYWLGKVPLGENIAD
                     PTVVFPHKTSEYMVYGRRNTENYIADQTYTISMKATKLTVQSFAVYIAAGRVKVGDMK
                     PTEGLANTWELTFTVTKQHIDSGVTNYLEIYQYPSATKGAVQIEWLKLEKGNTRTPNI
                     SEYKYRGTGMRDSNNPKDYVWDLAPEYVEDNLATDIKISEITGKANNYTDGKVSEINS
                     QLTASINEVDTTAKDAQTKANANATAIDELDNKIDERINDTATTTLTVTNGNTGSAKL
                     YREGKTVSIYFVALNGKSSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSI
                     GADGAIKWRRNSSYGSDYTFAITYTI"
     gene            120549..120995
                     /locus_tag="IXT53_000139"
     CDS             120549..120995
                     /locus_tag="IXT53_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4904003.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            120997..121134
                     /locus_tag="IXT53_000140"
     CDS             120997..121134
                     /locus_tag="IXT53_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HAQ4904004.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            121172..121465
                     /locus_tag="IXT53_000141"
     CDS             121172..121465
                     /locus_tag="IXT53_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4904005.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERRK"
     gene            121462..121686
                     /locus_tag="IXT53_000142"
     CDS             121462..121686
                     /locus_tag="IXT53_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAQ4904006.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            121683..122708
                     /locus_tag="IXT53_000143"
     CDS             121683..122708
                     /locus_tag="IXT53_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ4904007.1"
                     /translation="MKKKITITAMSLLTALFLLPINTFAYTINDEYNLAPNQGDSRLA
                     IPNKIILHETGIDAPARNVAANMKNNYNGSNPYTTDVIGDGGIVYRVGEQGYVSWGAG
                     NANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIIS
                     HLWVTNYVWGNHTDPYGYLSQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGA
                     ANPTLTDGTNHAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADG
                     IYRPDVNQAYNTSGNVGYHVSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRW
                     YLNIPKR"
     gene            122898..122971
                     /locus_tag="IXT53_000144"
     tRNA            122898..122971
                     /locus_tag="IXT53_000144"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:122932..122934,aa:Ile,seq:cat)
BASE COUNT        41162 a        20556 c        25747 g        36107 t
ORIGIN      
        1 ttttcagatc tgaacggttt tttttatcta ttcatcaaga tcaaatttca tttttcaatg
       61 attcaatcaa ttcgccctct gcttcatgac tatgttttcg gccattaaca agatagagtg
      121 ctgtacatat caatggaatg actccggcaa tcacatacaa tcctctaaat gaagtaagag
      181 attttagcat ccccatcaaa taaggaccaa cgcctaagcc taggtctaaa ccaataaagt
      241 aggtagataa agcaatactg attcgatgtt cgcggacgat ttttaaacaa acagcctgtc
      301 catttgacat aaacgtacca taacctaggc cgactaatgc tccagagata agcaatgtca
      361 cactattcgt cgttacactc agtaaaaata atcctgctgt taagaataaa tagctaggat
      421 acatcacgta ttgttctcct ttgacatcga aaatccttcc agtcaatgga cgtgtaaatg
      481 tgatgaccaa agcatatacg acgaagaaaa atgacccagc agcaactaag tggattactt
      541 ttgtataaga agataaaaat gataaaacac ttgaataaga aagacccatc aaaaaggcaa
      601 ttcctgtaat gaaaagtgct ttcttttcaa taaaactatt gatattccat gagcgaagag
      661 atattttttg ttcttcagat aatggaatat tttttacagg aaagacaagg caggcaatcg
      721 taacaaagaa gaccagtaca attgaaaacc agataatgaa acgaaaatca gtcatattca
      781 gcaaaatcat tcctaaaaat ggcccaacag cggcagctaa actagtactt aatccataat
      841 aattgatgcc ttcccctttt ttagatgcag ggatatatgc agtaacgata gcatttgtag
      901 ctgtagaaac tgtaccgtag ccgaatccat tcaagaatcg aactaaatac aatgccccga
      961 tagttggcgt ataaagatat gctaatgttg aaaggagata gaagatcgcg ccgcctctta
     1021 aggtaaactt ccgaccaaat agttctaact gtttccccat gaataagcgt gccagcaatg
     1081 ttccgatgat atagatccca gaagcaaaac ctgcttggct caagctggcg tgtaaggtgt
     1141 cttgcgcaat gactgccatg atgaccatca acagataata aatcaggtag acaataaaat
     1201 tgatcaatgt gattccaata aaatatttat taaataattt cttttccatg tatgttatac
     1261 attcccctta agctgaatat gcttttaatt tttgtatttg aacttctaca tcgtgataat
     1321 tggatggata gcgatcaaca agttggcgaa taaataaatc tcttgtagac ataaacagat
     1381 ttaaaaattc ttcgcattcg tttgttggat tctcatccac tgctttgatc aattcgatcg
     1441 agttttgata cgcatcattt aaaaagacta aagaagcttt catatcaaat cttgtttgat
     1501 atgctgtttc taatcgatta atgattgtgt tcatccagta attcatgttt tttccctcta
     1561 ttcttaaaaa attttacttc tgcagtataa cgaataaaaa aaaggaaaaa aatcgcataa
     1621 gtgatggaaa aaggtactta acaaatgtta atatcattag taaaatacct ttggacttta
     1681 aaaagtttta aagtactgta tgatcttttt agatgaaagt ttggatgggg gagcatcatt
     1741 ggatagtaga gtcttaaagg aatatttgga taaaaatgat tttccgatta tacgcaaagg
     1801 atatcataaa tatttgacat tcgaagggat ggaagattcc catacgtata tcttgaaaga
     1861 gggaacggta aaagcaagtg ttatttcaaa tgatgggaga gaatttaacc ttcgctatat
     1921 caaagatctt gaaatcgtct ctcttttaaa agacgaatat tcacaattca tcgattcgcc
     1981 gtataatatt cgagttgaat cagaatatgc agagttttat cgaatcaatc gtgtgcgctt
     2041 ttggcaaaat atcaaccaag acccaaagct acaacactat gtaaaagagt attaccgcta
     2101 tcgtctgctt tattcgatga aaaaaatgca gcaaatgcta ttaaacggaa agctgggagc
     2161 tgtttgtacg caactttacg aattatatga tttattcggt gtgcggacag aagaagggat
     2221 gctgatcgat ttttcgatca cgaatgaaga aatcgccaaa ttttgtggca tctcaactgc
     2281 tagcagtgta aacagaattt taaaacaact gaaaaaagat ggtattatca ccgttaaaga
     2341 ccatcatatc gttattttga atatggactt gttagaagac aatattatct tttgatgaac
     2401 aaagaggttg tgaaatcagc gttttgacct gagtattaga gaaacaaacg gaaaaatagt
     2461 tcctcatatt ttgccgtttt ttgagctaat accgagggtc agctgattga acaccgttta
     2521 ccaagaggtt gtgagcctgc cgtttaactc tgagtcacaa ggaacaattt caaaaaacag
     2581 cttttcatgt ttgatgaata ttgtgacttg tgaccgagga gttggtagga gaacaccgtt
     2641 taccaagagg ttgtgaaaaa gctgttctgc atcaagtaat aagaagagcc aagtaaaaac
     2701 agtctcttat gtttttaact tggctggact tatcaccgta gatgtaagct tttgaacgcc
     2761 gtttatttgc aacaaaaggg gctgtgacac gatttttgtc acagcccttt ttttgcgaac
     2821 attcatttag caatttttag aaacttctgc cgccaccgcc aaaagtcccg ccaccgctag
     2881 aatgggtagt acttccgcca ccgccgcttc caggaggtgg gcttttgggg attcttcttg
     2941 tcgtgacaaa agaatttgtc agacgatcgg ttttttcagt taatttgata ctggattttt
     3001 ctcggaatgg atatttgtag atgcctgatt tcaattgata acgcgaaaca gtcacaaaat
     3061 aaaagacaag actaaggata agagccaaaa caacagcaat gatgatttct actgtcgtca
     3121 aaaccttata gcgtgtaatt ttgccagttt cttcgtctac acgataatgt cctcctggaa
     3181 ctccgtcttc cacatatgtt gctgatttag acaggtaagc ttttgcagca ttgtaatagg
     3241 cagcatttgt catctgatca taaacatcat ctaagatcga atcgattctg ctatcagtca
     3301 aataatcgat catattacca gaagtcgaaa tgtagatttc acgctgtccc atatctaata
     3361 gcaatactga gccattttga tcatttccga ctgcgtctct taagtaatta tcagcaaagt
     3421 ctcttggttc ttctgaatta gatgtagtcg ttacgatcaa gactcgtcct ttgatttttt
     3481 cattcagctt ttctgcctgt tgttccaaag attggatttg atctgttgtg aataacccag
     3541 cctcatcatt taccgtagct gtatctgcat gagtagccac cggccagcca acacttacta
     3601 gcaaaagaaa gaataaaaaa aattttttca tcatatcaag taacctccga tcagaaagag
     3661 gatcgctagc ccagcgaaaa tcccaagagc ggtcagtccc aattttttct ggctgatcgg
     3721 caggacaccg ctgacctttc ctgtttgtcc attcattgcg tagtagtaga cttttttact
     3781 tgagtcattg cttcgataag taacaagcca taccggcagt aatacgtaat tatttacttc
     3841 gtttgtgatt gaagcagaag ttcgcacacc ggtcaatgtt gtatatccat ttgcggtatc
     3901 ttttaacagc ttctctgaat agtcttttaa ttcagattga atctgtgatt tgattgcctc
     3961 atattcgata tctctttttt ctgcttggaa cccggctaaa tactggcttt taaaatcaat
     4021 tgctttatct aacgggaaag gttgcacgcc ttcgaccatc ttttgttgcg tattttttga
     4081 taaggcattt tttactagtt ctttaaaaga aagcttgcct gctcttgaaa cagcaaattg
     4141 cttcgtttct gtgtattcga tgtcacctat ccgccatatg cggataactg taccattcgc
     4201 ctgcatggca ccatctactt cagcatctgt agcccaataa gggaaataca cacctgtcaa
     4261 cttatcgatt tgatccttat tgaaaaaagc tttgggaaca aaccattttt tcttggtcca
     4321 agcgagaaat ctgttgattg cttcttcttt ctcgacagca aaaggaagta ctttatttgg
     4381 caagaattct ccgcttaaac gtcccgatag tacgacggga ttatggcagt agtaacaata
     4441 agtcgcagca gttgtcgcat cagtcacgat ctctgcacca cagttgggac aaagaaaaag
     4501 atctatcgct gcggtattat ctgcagattc gtccgctgta tctgaaaaac cgcctgcctc
     4561 gtcaaccgct ttgcgctcgg cttcattcat atcggcaagc tgttcttggg ctgtaaaagt
     4621 aaaatcttcg gtaggtgaag gttcttcttt gctttcttcg ccagccatac gcgcttcttt
     4681 ttgcttttgc tcatattggc tgacttcttc ttctgtatag atattcaggc agtattcgca
     4741 atgaaatttt tggtctttgg gatcaaaagt caacggaccg ccgcaattag gacatttatg
     4801 tgtaagaaca tccatcagct gcacaccttt ctaaataaat taattaacgg ggattccttt
     4861 aaacggttta ccacagtttg gacagaattt agggatgccg ttggaaagat ccacgatctc
     4921 attgcattca ctgcatcgca tttgtagctt aggctgaacg ttttctacag gtttggcagc
     4981 tccgcagttg gaacagaatt ttccggaatt ttctgtcccg caaactggac atgtccaagt
     5041 atccgcattt ccttgtgcag tttgttgatt ttgtttttct gcttgttgtt tgatttgttc
     5101 ttggttattt tgagctgctt gagaaagata agaaccattt gcattcattc ccatacctac
     5161 acccataaag ccggtcattg ctcctgcttc attttttccg gctgcttcca taccacgtgc
     5221 aatggaacct tgcacgtatc cttcacgtac gctaggatca cctagcatcg ccccttcgtt
     5281 acgcatattg atcagtttca cagaatcatc ggtgtaagaa atgctggcta cagccacaga
     5341 aacaatctcc atcccacgta attcacgcca agaatcatcc aatgcagtat ccatgtattt
     5401 gcttaattcc aaactcttag atggtacata agaaatccgt tgaccatctg cagacatctg
     5461 attgattgct gattgtagcg ctgttaggaa ttcagctaaa tactgctcat tgatatcatt
     5521 gatttccact tgtgttttgt ttttagggat tgcgtttgta taaaataaaa tcgggtcaac
     5581 gacatgaata gaataagtgc catgtgcccg taagaataat tctgcgttat agaaattgtc
     5641 aaagtagttc aacggcgcgc ttgtaccgaa tttgattccc tttatttctt ggagattgat
     5701 ataaaaaact tgttgtttct gcggtgtgac gccgccaaat ttgaatcggt caaaagtttc
     5761 agcaattgct tctttcagtg ttccattgaa catggaagga gctgcgtcat ttttaatggt
     5821 gtaataacct tcctcagcgg tataatcgat gatttttcca ccatcgacca aaagcatcat
     5881 catgtttgga tagacatgga cgactgttcc gtctgttaaa acatcttctg ttcctttacg
     5941 attggagcca cgtttgtcat ctttgcggac tttgacacct tttgtcatga ccgttgtatc
     6001 actcatattg tctggttcga tgacttctag ccattgatct gccagaccgc cgccaaccat
     6061 acttgttgct gctttgataa gacccataaa aaattcctcc tctgattcag ctagtgccaa
     6121 acaattattg tttatttatt ataccatagg aagatgtgaa acttctcgga ctagagacgg
     6181 aattgaaatg gaaaattgcg tattgtttaa atacctgaaa aagccgagaa ggatagtagt
     6241 ttttttacct aatatcacgt ataataagag atagtgaatt ttttaaaaaa gggggatcct
     6301 tatggcatat caagcacttt atcgagtctg gcgttcgcag cgtttcgaag acatcgttgg
     6361 acaaaaagct gtgacacaga cactaaaaaa tgctattgtt tcccacaaaa cctcacatgc
     6421 ctacttgttt acaggaccca gaggaaccgg aaagacgagt gcagcaaaga ttttcgcaaa
     6481 agcaatcaac tgtccgaaca gtaaagacgg tgagccatgc aacgaatgcg aaatgtgtcg
     6541 atcaattact gctggtacgc aagaagatgt gatcgaaatc gatgctgcca gcaataacgg
     6601 agtcgaagaa atccgattta tccgtgatcg ggcaaattat gcgcctacaa aggcagcgta
     6661 caaagtgtac attattgatg aggtacatat gctttcaaca ggagctttca atgccttgtt
     6721 gaaaacattg gaagagccaa aagaaagtgt catattcatc ttagcaacga ccgaacctca
     6781 caaaatacca gctacgatca tttcacggac tcagcgattt gattttaaac ggatcaatac
     6841 ggcagatatt gtcgaacatc ttgaatttat cttgaaagaa tcgactatca gctatgaacc
     6901 agctgcattg aatgtgattg cccgttctgc agaaggcggg atgcgggatg cattgagtat
     6961 tcttgatcag gcaatctcgt ttagtgaagg aacaattact ttagatgatg ccatgcaagt
     7021 gacaggtagc ttaactgacg aaatgatgga cagttacttg agtgcttgcg tgaaaaaaga
     7081 agtaccagaa gcattggaaa cattgaacca aattctcgca gccggcaaag aatcacgtcg
     7141 ctttctagag gatatgttac aatactgccg tgacttgctg atgtatcagc aagcacctaa
     7201 cctagttgag gaacaatcga gtacgatcag aagtacattt acagaattgg cacaaactgt
     7261 ttcagcggat cagctgtttt caatgataaa gattttgagc gatactcaaa atgaaattcg
     7321 ttttaccaat agtgccgcta tttatttgga agtcgctaca gtcaaacttg cacagctacc
     7381 gtctgcttct gttccttcag cagatgtagc atttgcagat gatcagatag taaaacagct
     7441 acaacaagag attaaggaac taagaaacga aatcaaggaa ctacgcagta atacaggaat
     7501 tgagaataca aaagtacagc cggtacagca aacaaatcat aaaaaacaga caagtaccta
     7561 tcgtgtaccg acggaacgag tatataaagt tctgcgggaa gcgacaagac agcatctgaa
     7621 ccaagtaaaa gaagtatggg aagacttgct gatgagtcta tctgtcactc agcgagcaat
     7681 gcttaaagca agcgaaccag ttgcagctgg acctacagga ctagtgattg cgtttgatta
     7741 tgagattgtt tgtcaaagag cagcaaatga cgaagaattg cagctttcgg tacacaacaa
     7801 cttaagccga atgatcaaag attatgcgcc tgatcctgtt tatattacaa gagaaagctg
     7861 gccagaactt cgaagaaatt atttgacgca ggcacaagaa gagggaacag aaaactcttt
     7921 tgcttctgaa gactcagaag gagaaatcga gttgcttcct caagaacaag aagtaccaga
     7981 agaaacactt gtagtaaaag cagaggaact gtttggctcc gaagctgtta cagtggtaga
     8041 tgattaataa aaaggagtag ataattatga tgcgtggaat gggcaatatg caaggcatga
     8101 tgaaacaagt acaaaaaatg caaaaagaaa tggcagaagc acaagagctg ctaaataaaa
     8161 cagagtttac aggtaaagcc accaatgaat tggtcaaagc ggtttttaca ggcgatcgtc
     8221 gcatgaaaga tatccaaatc aatgaagaag tcgttgatcc tgatgatatc gagatgcttc
     8281 aggatcttgt cgtaatggca gtcaatgatg cgatcgcaca aattgatgtg gaaactgaga
     8341 aaaaattagg gaaatacgca aaaggtttgc caggatttta gttttaaact gaaggagtaa
     8401 tgaatatgca atatccagaa ccaattgcga aactgatcga cagctacatg aaacttccag
     8461 gaatcggtca gaaaacagcg acaagactcg ccttttatac gatcgatatg aaagatgaag
     8521 tagtaaatga atttgctaaa tctttgttaa gtgtaaaacg cgatttgcat ttttgtagta
     8581 tctgcggaaa catcacagaa gaagatccct gcgaaatatg tagaaatcct tcacgtgatc
     8641 gaagcgttat cctagtggtc gaagagccga aagatgtaat ggcaatggaa aaaatgcgag
     8701 aatatcacgg actttatcat gttctccatg gtgtgttatc accgatggaa gggacaggtc
     8761 cagaagacat caatatttct tctctgttgc agcggctgca tgatgcagat gtaaaagaag
     8821 tgattatcgc aacaaatgca acaacagagg gagaagcaac agctatgtat ctctcacgtc
     8881 tgatcaaacc cgcaggtatc aaagtgactc gtctagcaca tggtctgtct gtaggctctg
     8941 atatcgagta tgcagatgaa gttactttat tgaaagctgt cgaaggaaga cgtgagttat
     9001 aaatttctaa ttaagtgttt tttcagatac aatataaaag cagcacaaga gatgggggca
     9061 acaatgtgaa cggactattt attacaatcg aggggcctga tggtgcagga aaaaccagca
     9121 tcttgaatga actttaccct cttttgaaag aagtagcaaa aacagaaatc attcaaacaa
     9181 gagaaccagg aggcattccg attgctgaag agatccgagc agtgatcctt gatccaaaaa
     9241 atgatcggat ggatgaacgt acggaagcac tgctttatgc agcagcaaga agacaacatc
     9301 tagtcgaaaa ggttctgcct gctctggcaa aagggaaaat cgttttatgc gatagatttg
     9361 tcgacagttc tcttgcttat caaggtgcag gccgacgcat cggcgttacc gagattgctc
     9421 ggttgaatga gtttgctaca gaaggtacta cgccggattt taccctttat cttgacgtag
     9481 attcagacac cggattaaga agaatcaaga aaaacagaca aaatcaaatc gaccgtttag
     9541 attcggaagg tttggaattc caccagcgtg tacgtcatgc ttacttaaag ctggcagaag
     9601 agaatccgga aagaatccac aaagttgatg caagaaaaag ctttgaagaa gtgctgcaaa
     9661 caagttatca tacaattatt gaacagtatc cgcaattttt tgaaaactag gaaggtgaac
     9721 gtttatgaaa attattttag cgattatcca agacaaagac agcaaccgtt tatcaaatga
     9781 attgattgat gcaaatatcc gtgccacaaa actatcatcg acaggcggct tccttaaggc
     9841 tggaaacagt acgtttatcg tgggtattga agatgaccgt gtagaagaag cattggaaat
     9901 catcaaaaaa acgtgtgaat caagaaagca atttgtatct acacctgtta cactggatat
     9961 ttcgatggat gggggcgtcc cttatccagt agaagtagaa gtaggcggtg ccacagtctt
    10021 cgtcttacct gttgaaggat tccatcaatt ttaggaggag ctgagctatg aatcaagaaa
    10081 taaacttggc tcaaatgcaa ccgatcgtgt atcagcagct tcaacggagt tttgagcatg
    10141 ggcgtatcgc tcatgcttat ctttttgaag gggagaaagg aacaggaaaa catgaagtag
    10201 gtatatggct tgcacagcac ctgttctgta cccaaatgaa agacaatctg ccttgtggaa
    10261 aatgcaacaa ttgtcagcgg atcgaaaaga aagaacatcc agatgttttg gttattgaac
    10321 cggaaggaca aaccatcaaa gtcgatcaga tcagacgttt gcagacggaa ttcagtcgca
    10381 gcggttacga atcaagaaaa aaagtttttt tgatcaaaga agctgaaaaa atgaatagca
    10441 gtgcggcaaa cagtttgttg aaattcttgg aagagccccc tggagatttt ttagcggtgt
    10501 tagaaacaga tgcagtagga agaattctcc ctactattca atctcgttgt caagttctgc
    10561 attttcaaga actttcgaaa gaagcactga tccatcgatt gcaagaagag cagattccat
    10621 tggaaaaagc aaaacttttg gctttcttaa caaacagttt aggaaaagca gttgaaatct
    10681 ctcaagatga atggtttaat gatgctaagg acttgattta tcaatggttc gtttacttgc
    10741 aaaagcaaga tactcaagcc tttatctatg tccaaaaaaa attggtaaaa tcatttaaag
    10801 aaaaaacaca acagtttaca ggattatctg tcttgatgtt ttattatcag gaagcacagc
    10861 gtcaagcagt agcaaacggc gaatatggca aagtgaaaca gataaaccag ataattgaac
    10921 ggatattgat cgcagagcag aaactacgca gcaatgtcag ttttcaggct gttgcggaac
    10981 agttcgtttt gcatacgatt ttttagcttg ccgcaaagga tgatgatcaa ggaggatgga
    11041 aatggtagaa gtagtaggtg tccgatatag agatgcaggt catatctatt attttgcacc
    11101 aggagactct gaatatgttt atagtgacaa ggtattagtc gaatctcagc aatccttaca
    11161 tattgctact gttgtccttc ctaaaaaaga gattgatccc aatgaccttc ctgaagctat
    11221 ccgtccgatc ttgcataaag cgtcacaagc tgaattagaa aaagacaaaa aaaacaaaaa
    11281 agatgcttat gatgcattga aaatcgcaaa agaaaaagtg cagttgcgtg agttggaaat
    11341 gaaaatggtc aaagccgaat acacatttga tcgaaataaa ttgatttttt ctttcacagc
    11401 ggacgggcga attgacttta gagaattggt aaaagatctg gccgctgtat ttcgtactcg
    11461 tattgagctt cgacaaattg gtgtccgcga tgaagcaaag ataataggcg gaattggtcc
    11521 atgtgggcgt ccattatgct gttcttcttt tttaggcgat ttcgtgcctg tatcaatcaa
    11581 aatggcaaaa gatcaaagtt tatctttgaa tccaacaaaa atttccggtt tatgcggacg
    11641 cttgatgtgc tgtctgcaat atgagagcga agaatacgag acagcgaaaa aagaactccc
    11701 tgactttgga aaagtaattg ctacacctga tggtaaaggg aaagtcgtag ggttgaatct
    11761 gcttagccgt atcgttagta ttcggctgac tggacgggaa aatcctgtcg aatatgaatg
    11821 ggatgaattg aaagaattta tccaagtagg tgagatgaat ggataagaga tcattatacg
    11881 acggattgaa tcaattagaa tcagagttga gaaatacatt aacagaactt gttgaaatga
    11941 agcatgcttt gcatgagatc gtagaaaaaa atacaacttt ggagatggaa aatcaacgtc
    12001 tgcgggaaca cctgcgggaa ttgaaccaag ttgcacaagc tccagctgaa aatgtgaagc
    12061 aggaactttc taagtctcgt atgaatcttg aaaaaattta tgaagaagga ttccatgtct
    12121 gctacgattt atatggatca agaagggaca atgacgagcc ttgtgcgttt tgtctagaag
    12181 tgatctatcg cgaaagcggt cgatgaccaa taacaagagg agactggagc ggagaaatac
    12241 tgctccagtc tttttattaa ggagattgtg acagatgtat aatcaaaaaa gtttcaaggg
    12301 aaatccggta tatggggatt tatatcttgt accgacgcca attggaaatt tagaagatat
    12361 gagctacaga agtgttcgta tgctgcaaga atccgatctt atcgcaagtg aggatacgcg
    12421 taatacacaa aagttattga atcattttga aatccataca ccccaaaaaa gtttacatga
    12481 acataattat aaagaacgtg tccctcaatt gattgctttg ttaaaagaag gaaaaacgat
    12541 tgctcaagtc agtgacgcag gaatgccttc catcagcgat cctggacatg agctagtttt
    12601 agcctgcata aaagaaggga tttctgtgat ctctatccca ggacctacag ctgggatgac
    12661 ggctttgatt gcttcagggc tagtgcctca accattttta ttttatggct tccttccacg
    12721 taagaaaaaa gaacagaaag aaacattgaa aaagctaaaa gaacagacag ttacgctgat
    12781 tttttatgag tcaccttatc ggatcactgc tacattgacg aatttcttag aaatttttgg
    12841 aaatcgccaa gtcgttttgt gtcgggaatt gacaaaggtc catgaagaat accttagagg
    12901 aagtacggaa gagctactcg cttatatcga agaacatcca gtaaagggtg aatgttgtct
    12961 gcttgtagaa gggaatttgt tcgaaatgcc agtgacggaa gttgatgaag agttgggaac
    13021 gttaaaggaa caagtagaag caaagatcgc ttcaggcgaa aaaccaaatg ctgctatcaa
    13081 agcagtagcg ctgaaaaatg ggctgaaaaa acaagaagtc taccgtcagt accatgaatt
    13141 agactgagat agtataagtt gtctataatg aaagactaat gaggaaatca gctactaact
    13201 tttcaaatct cttgaacgcg aacgaatgtt cttgtataat gaataagagg tgaggatgat
    13261 ggagctgcaa tttcctttac ataatgatac atcaagaaaa atcattcata ttgatatgga
    13321 tgcttttttc gcttcggttg aagaacgaga tcatcctgag tttcgcggac atccattagt
    13381 aattgccaga caccccagtg acactggtgg gcgtggagta gtgactacag caaattatga
    13441 agcaagaaaa ttcggaattc attcagcaat gagtgcacaa aaagcgtatg agctttgtcc
    13501 gcaagccatc tttaagcctg gcgaccatca taaatacagc gatatttcca aacaagtacg
    13561 ggaagtattc tatcgttata cagatattgt ggaaccagtt agtatcgatg aagcgtatct
    13621 tgatgtgacc gaaaataaaa tcaattgcaa gtcagccatc aaaatcgccc gaatgatcca
    13681 agcagatatt tgggagagtg tccagctaac atgttctgcg ggagtaagtt acaataagtt
    13741 tttggccaaa ttagcatcag actatcaaaa acctagagga atcacggtag ttgcccctca
    13801 agacgctgtg ccatttttaa aagctttgcc aattgaaaaa tttcatgggg tagggaagaa
    13861 aactgtacct agaatgcatg agctagggat ttatacagga aaagacctat atgaatgcac
    13921 agaaatgatg ttgattcgaa attttggaaa aatgggctat tctctttatc gtaaagtcag
    13981 aggaatccat gattctccag taaacgttac aagagagcga aaatctgtgg gaaaagagca
    14041 tacttatggg cagccgttgc aaacggaaga agcagtgctg actcagttgc gccaactagc
    14101 tgaaaaagta gaagaagcac tgagaagagt ccaaaaacat gggaaaacag tagttttgaa
    14161 ggtcaggtac acggactatt ctactgtgac caaaagagtg acattgccag aatatatcta
    14221 taaaaaagaa gcactctttt atcaagcaag ccttatatgg gaggaaatct tgggagtaga
    14281 aaaaggaatc cgattgttag ggattacttt gacgaatctg gacccgatga cttatgaaaa
    14341 catcgtcctt ccattatggg aaaatcaaga aatataaaaa cagctttgag agttgaaacc
    14401 ggaaatccgt tttctctccc aaagctgttt ttatatcaag tcttgctttt tgatttgttc
    14461 ataggtttct gttgcatccg tacatttttc ccaaatcaca acttgattgg attctaaaga
    14521 tttttggacg tcaatgatcc gctggttgct acttccgcga aattgtagtt ttaggtcacg
    14581 tttgcttaat tcaaagcgtc cgtcaacaag gatatcgatt tgcgaaagga gttctaattt
    14641 atcttctgaa tcttgtagca attcatcaaa ggtatagcct gtccatgacc agatatcttt
    14701 tgtcgtaccg aactcttttc ggatacgtcg tacgacttgc agacaaacat ctgtattcaa
    14761 aaaaggttcg ccaccaagta aagttaatcc ttgcacgtaa tcgttgcgca agtcagcgat
    14821 gatttgttct tctaattcag gtgtgaatgg tttgccatat cggaaatttt gtgcagcttt
    14881 gttgaagcag ccttcgcagg caaataagca gccactaacg taaagactgt tgcggacacc
    14941 ttcaccatcg acaaaattaa acggtttgta gtcagcaata tagttttggc tgtagtcttt
    15001 tgctaaccat tcttttggtt ttggatttct cataaaaaaa cttctttcat ttaaggttca
    15061 aagatatagc tattgaagat aaaatctact cgaccacata aataagaaca aactgaccta
    15121 aatccattgt tcaagaaagc ttgttcttat tttgatagag ggcgttcaaa gatacaaagc
    15181 catcgaaaga gggtggtgaa aaaagacttg tcatagcctc ttattctgaa taaacggtgt
    15241 tcaaaagctt gcatctgcag taataagccc aactaaacga aaaatatgag gaactatttt
    15301 tgtttagcta ttcttattac ttgatgcagg acagcttttt cacaacctca tttcatgtgt
    15361 ttcacacgtg aggaaatttc tttatggcgg ccatgtacca tcggacgagc ttgcggattt
    15421 cctaaatagc cacaagtacg tttgactaca tcacatgttt ttggatcatg atttccacat
    15481 tctggacatt cgaaccctcg ttctgtcggt ttgaaatcac cttcgaaacc acattgatag
    15541 caatgatcga ttggcgtatt cgtaccaaga tacccgattt tgtcatatgc aaaatcccat
    15601 acagcttcta aagctttcgg attttgttgg agcatagggt attcacagta gtggatgaat
    15661 ccacctgaac aatattttgg ataatccttc tcaaattcca atttttcaaa tggcgttggg
    15721 tttttacgca catcatagtg gaaactgttt gtataatatt ctttatctgt gatattttcg
    15781 atgatgccga atttttctgt atccaaacgg cagaaacggt cagttagact ttcacttgga
    15841 gttgaataga cactgaaatg ataaccatat tcatcccccc atgcatctgc attagctttt
    15901 aattctttga cgatatcaag tgtgaaagct tttgcttctg ggtttgtttc ccattcgcca
    15961 ccgaagaaag ctgaagcaac ttcatacaat ccaatgtagc ctaacgatac agtagctcga
    16021 cggtttttga ataattcatt gacagaatcg gtacgtgaaa gacgtttccc gaaagcacca
    16081 tacatatata aaattggcgc atttgctgga atggcttctt tacaacgttc tacacgatag
    16141 agaagcgcat ctttcatgat ttctaagcgg tcagacagta actgccagaa tttattctga
    16201 tctccttttg attcgatcgc gattcttggc aaattcaatg taacaacacc taaattcatt
    16261 cgtccgacat ttacttcttg accattttcg tctttccacc cttgcaagaa tgaacgacag
    16321 cccataggta ctttgaaact tccagttaat tcaacgattt tatcgtaatt cagaatatca
    16381 ggatacattc ttttcgttgc acattccaat gctagttgtt tgatgtcgta attaggatct
    16441 tcagggttta agttcacacc ttttttcaaa gtgaagatca gtttagggaa gatagctgtc
    16501 cgcttttcac tccctaatcc attgattcga atctgtaaaa ttgctttttg aatttctctt
    16561 tcaaaccagt tttctcctag tccaaatcct aatgaagtga aaggagtttg tccatttgaa
    16621 gtaaataagg tattgatttc atattccaaa ctttgcatag catcgaagat atcttttttg
    16681 gtttttgctt ttgcatattc tttttggcgt tctggcgaat cgatccattc ttccgcgtct
    16741 tttagatgtt ttttatagtt caattctgca aaaggagcaa gcaattcatc tgttcggtcg
    16801 gcagaacagc caccatattg acttgacgct acattggcaa taatctgtga gatttgtgct
    16861 gtggcggtct ggatagattt tggcgattcg acttcggcat tgccgatttt gaacccattg
    16921 ttcagcatgc ctttgaaatc aatcaagcag caattcgtca tcggtgtata aggatgataa
    16981 tccaaatcat ggtaatggat atcgcctttt tgatgcgcat ttgctatatg tggtggcaac
    17041 attttcagtc cgattgattt tccaacgatc ccggctgtca gatctcgctg tgtattgaaa
    17101 acattactgt ctttatttgc gttttcgttc acgacagttt ggtccttgtt gattagcttt
    17161 tcaatcgaaa agttgatatc tgttgcttta ctgcgagcaa aatctcgttt ggtacgataa
    17221 ttgatgtact cttctgctaa ttcatattcg tttttttcta gtaatatatg ttccacgatg
    17281 ttttgaatct catagatttt gacattttca gcaaaccggc ttttgatttc ttggacaacc
    17341 aagtctgcaa tcatttgaat tttttcataa gtaagtggtg ataatgagcc gtgaattttc
    17401 ttttctgctt tgatcagtgc ttcattgatc ttttgttcat cgaaatcaac atggcggcca
    17461 tcgcgtttga tgactttcat ctgggatgtc tctggctgct gagtaacgac agttttttct
    17521 tcataattta ctttcattat cttcaactcc tgctaaattg ttcttgtcta tcataaaaca
    17581 aaatcgggga aggagcaact aaatatcggc tatatatagt attcaatgtg ctgtataatt
    17641 ggatttctat actatatgta gtgtatattt tattgtgaaa aaaggtgaaa acttgtagct
    17701 ggtggatttg ttttttgcat atttaatttt gctgaataaa ataattgaat aaaatttttt
    17761 attcagtcca agtaaaagtg acaaaaacat aagagcaaat tattcgtaga tatgctacta
    17821 tgcaaataaa tagaaatgaa aaatgaaaga ggaggagaag aattggaaaa gatgatcgaa
    17881 gtgaaacatc taagtaaaag ctacggcagc aaactttctc ccgtcaaagc cttagataat
    17941 atctcattca cagtagaaga aggcgagttt gttgggatta tgggcccaag tggtgcagga
    18001 aagacaacgc tgatgaatat cttgtccacg atcaatttgc ctacgatggg cagtgtctcg
    18061 atccaaggaa aagaaatcac aaaaatgaaa aacagtgagc tcagtgactt tcgtcgaaaa
    18121 aaactaggat tcatttttca agagttcaat ttgatcgata ccctaaatgc aaaagacaat
    18181 atcctattgc ccctagcagt tgaacggatg actaaagagg aaatggaaaa gagagtaaga
    18241 catgtagctc aattgttgaa tatagaagaa ctgctcaaac ggtatcccga tgaactttct
    18301 gtcggacaga gacaacgaat tgcagctgct agagcacttg ttgttcagcc acaagtgatt
    18361 tttgcagatg aaccaactgg ctctttggac tcgaagtcgg ctacagaatt gttgaactac
    18421 ttgaagacca tgaatcaaaa agaaaaagca acgatactgt tagtcaccca tgatccatat
    18481 acagcaagct actgcgatag aattttattt attaaagatg gtgtgatttt ttctgaagtt
    18541 gtcagaagag ggacaagaag agagttcttt gagaaagtaa tagacatgca ggcgacgatt
    18601 ggcggaggag gaaaaatgaa tgctgtataa actagccgtt aaaagttttt ttcggcagtc
    18661 acgaggctat cttgtttatt ttttcagttt gactttatcc gtaatgatct attattcctt
    18721 cagcgcaatg acctacgatc aatctttagt cagaagagca agtcaagata cttcgataga
    18781 tggtggattg agttttggtg gatttatcat tactgttgtt ttgctttttt tcgtattcag
    18841 tgccaaccga tttttcttga atagaaggca aaaggaaata ggcatctatc agctttttgg
    18901 tatgaataaa ctgcagatat caggtatata tgtgttagaa atcatgataa ttggattgtt
    18961 cgcctgtatt tctgggatat tactcggtat tattttttcc aaactctttt caatgatttt
    19021 ggttcgaatg atggatatgg atcttactag tccatttttt atctccattc catcagtagt
    19081 agatacttta tttgcttttt tcatcatcct tttagcagta tctgtatatt caatatggaa
    19141 aatatggcgc tatccaatga ttcgaagttt tggagaaaaa gaacaagcag atagttcaac
    19201 aatgcgcttc aggacaaggc accgattact cgcagtcatc gggttactac ttttaacgag
    19261 cggatatttt ggagctagtc attttaggga aatcacttca tggatgatca gttatcatta
    19321 tcttgataca ttagttttct tttttccttt tttaattttt ttcgtttgtg tggtcggtac
    19381 ttatttgttt ttctgttata catttcggtt atttttgatc atattgagta aatggaagat
    19441 ttattactat ggtatcaatt ttttgataat gggtaacacg caagttcatt tgctcaaaag
    19501 ttggcgtaca aattctctga ttacagtcat tctcggaatt tctttaatga caattggcgg
    19561 cacggctagt atctctacta ttttatccta ccaggagaaa gtttcctccc cgatggacta
    19621 tcagtttgat gttgagacag agaaaaaaat caagccgatt ctagcagagg aagggcagaa
    19681 gataactaaa gaaatcacct tgcattataa agtcattggg agtatctata gtcaaaatct
    19741 cgaagaacct atgtttcaaa ctgctaatct gatcacggaa acacaatatc aagccttcag
    19801 aaaagttaat ccagatttgc cgaacatttc tttgaaatca cctggtcata ctgttttgct
    19861 tgatcaaatg caaactcttt ttcggaatat ttcattatat ggatctagta ttggactgcc
    19921 agataatcaa gaattgaaga ttcaacaaat gcttccaagt gtattaggtg acgaaacaat
    19981 ggtatatcta agtcctgtac tgattgtaga tgataccgtt tttgaaaaag caaaaggtgt
    20041 ggattatcaa gtagtcaatg tagatgtatc aggtggaaat aatgaaaaaa tagatgaacg
    20101 actgagcaat gacctgtcga taaagtggct caatgctgtt tattattctt atgatatcgt
    20161 aaatggaagt ctacaaggag aaattagcac aaagaaactt ccggatgacc aaatgaagga
    20221 agcacaattt agccaagaaa gctctcgttt gaatttctct tccagctatt cgaaattgcg
    20281 tgcagttaat cggcgaatcg gtatcaatat ttttgtcaca ctatttgtcg ggatgatcgt
    20341 gattattgct actggtagta ttttgacagt ccggcaatta gcagaagcag aggaagaaaa
    20401 agaaaattat cacctattga ctaagctggg gattccacaa agaaggatca gacggatgat
    20461 cttcgagcaa aacgcactta ccttctttcc acccatgata ttagggatca ctcacgcagt
    20521 atttgcaatc aatgtcttca ctcagtatat aaaaactgcc gattactggt tggcttatct
    20581 tgtgagtggc ttattgatcc ttatctatct tttgctttat gtcattacga gtcagttata
    20641 ttgtcggatt atcgaggaat gatatacgga ataagacaag ataataaata gaactaaatt
    20701 ttgaatacca tttatttgga ataagaggtt gtgacacgat ttttgtcaca acctctttat
    20761 tttttatatt ttttaaaagt acgagaaatt ataaagaaaa aagcagtgat ttgatgtgct
    20821 atttttataa aagctatgtg acaaaactgt tagtaaaaaa acggtttctg taagctagac
    20881 acatgtttct ttttgtcaaa attagttaca ctttgtgtga ggttatatga ggagggaatc
    20941 caatgaacaa atggcgttct aatccaaaat tgaattattg gggaaaattc atcattcgaa
    21001 caatttttta tagtgcagta ctgctgttcc tgatttatct gtatcactat aaaaatattc
    21061 aaggaggaac ttttatctat aacgaatttt aggaggaaga gtatggaaat caaaacgatt
    21121 attgaagcaa tcgatgattg gggaataaat gaacctgatc gagttgctta tcaggcggat
    21181 gagacgcata cttttggtga attgaaacaa gcatcagacg cacttgctta ttatttagaa
    21241 aaaaaggtag aaggcgatgg accaattgtc gtttttggca atttggagtt tgaaatgatt
    21301 gtctcatttc taggggttgt caaatctggc catgcctatt tgccgattga agagcataca
    21361 cccaaggagc gtatcctatc gatctttcgg gtggctaaac catctatggt catcagtatc
    21421 ggggactgga tagaagatat tccttcggtt cctgtcataa caaaaaaaga atttcaagaa
    21481 atcaagaaga taccagtagg attttatccc agaaatagcg taaaaggtac agaaaatttt
    21541 tatattattt ttacttctgg tactactgga gaaccaaaag gcgtacaaat cagtcatgat
    21601 aatctagtca gctttgttca atggacgatg gaagactttg ggatcaagcc tggtatgcat
    21661 ttcttagccc aagcgccatt ttcttttgat ttatcggtat tttctattta tcctgcatta
    21721 gtttctggcg gaatgctgaa gccgttaaat aaagcagttg ttcaagactt tcgtcaatta
    21781 ttcgcgacat tgccagcctt gaagttgaat gtgtgggttt ctactccatc atttatggat
    21841 atttgcttga tggaacctac attcaatgga gaaaatgtgc cggaattgga catgttttta
    21901 ttttgcggag aagaattgac gaaaaaaact gcagaaagtc tacttgatcg attcccaaat
    21961 gcacggatat ataatactta cggtccaaca gaagcaactg tagctatttc aagtattcag
    22021 attgaccagc atgtactaga tagttatgac cgactaccga ttggctacgt aaaaaaagac
    22081 acgaaagttt ctattgtcca agaaggtaaa cctgtttcaa aaggagaaac gggtgaaatc
    22141 attattgcag gaccaagcgt atcaaaaggt tacctgaaca atcccgaaaa gacagctgct
    22201 gcgttctttt gtcaaaatga cttagcaagt taccatacag gggatgcagg tagactagat
    22261 gaatatgggc ttttatttta tgaaggacgt atggatcagc aggtcaaatt acatggatac
    22321 cggattgaac tcggggatat cgaacattat ctgctccaag acaaccgaat caaacaagct
    22381 gtcgtcgtac caaaatacca aggaacaaaa gtacagcagc tggttgcttt tgtcgtatta
    22441 gaaaacaaaa cgaaacagcc ggattttcag ttgactaaat ctatcaaaga acaattacta
    22501 gaagttgtaa tggattatat gattcctcag aagttccatt atgtcgatct gctgcctcag
    22561 acagttaatg gaaaaatcga tcgaaaaaaa ttaacagctg aggtgaatcc tgaatgatgt
    22621 tacctcatat gatcccttat gcagagccgt tttatttttt tcttttagcc attgcgcttg
    22681 taccgatcgt tctatcttta ttgatatggg aaaaaagatt accagtttat caatcagcag
    22741 tgacactttt ctttttattt gtcagctttg gaggtaatta ttggcaacag ggagcggcgc
    22801 tgattgggta tgttttgtgg cagacgatac ttgtatgggg ctattttgtt tatcggcaaa
    22861 ggaaaaatca gacaggatgg ttttttgccg ccgttatttt agcgataatt ccactgtttt
    22921 taacgaaagt gactcctttt tttgaacatg gaaaagcatc gttacttggt tttttaggaa
    22981 tctcttattt aacctttaaa tctgtacaaa tgatcatgga aataagagat ggcatgatca
    23041 aaacatataa gccatttcgt tatatccagt ttctgctgtt ctttccaaca atctcttctg
    23101 gaccaatcga tcgctatagg agatttgaaa aagatcagct ccatccgcct gttcgtgata
    23161 aatatgttga tcttttggga aaaggcgtcc aaaatatttt tgtcggtttt ttatataaat
    23221 tcattatcgg ttattatttt ggtcaagtac tgcttcctgt agtgggacgc attgccttac
    23281 agcatggtgg tatttcatgg gcattagtag gttatatgta cgtgtacagt atgtatttgt
    23341 tttttgattt tgctggctat agcttatttg cagtcggtac aagctacatg atgggatatc
    23401 agacaccaat caatttcaat aaaccttttt tatcatggaa tatcaaagaa ttctggaatc
    23461 gttggcatat gacgctgtcc ttttggttcc gtgattatgt atatatgcga ttgatgttct
    23521 tcatgatgaa gaaaaaattt tttaaaagca agatcgtaat gtcaaatatc ggttattttg
    23581 ctttgtttct tctaatgggt atatggcatg gattgacgtg gtactacatc gtatatggac
    23641 tgtatcatgc ttgtctgatc tgtctgacag atgcatggat ccgatttaag aagaagcata
    23701 aagagcagtt gccttctaac aaatttacgc atggcttagc cgtatttttg acatttcaag
    23761 cagtatgttt tagcttcctg attttttcag gatttttaga taaacttttt tttagctaac
    23821 gaatagtgac atattaattt atataacaaa ggagaaaagg aaaatggaag aacaaatttt
    23881 atcaatctta gaagaaataa caggaacaga tgaagtaaga aaaaatcgtg atattgatct
    23941 atttgatgaa gggttgcttg attctttagc atctgttcaa ttattagtcg agctagacgg
    24001 agagctggat atccaagtcc ctgtatctga attcgagcga gaggattgga gcacaccgaa
    24061 taaaatcatt caacaagcaa ctgcattgaa gggttgagta tgaaaaagaa actttttgct
    24121 attttcggcc cggttgtttt agctgttggc ttactgtttg tgttttttac ctcctcctat
    24181 caagtagata tctctaatcc agaaacgatt agacgtgcct cttcttccat gtcagtgaac
    24241 gtgttgaaag gagatgcgat aaaaaattat gcattatctg aaaaacaata tattcctttt
    24301 tttggctcaa gtgaattaag cagaatcagt ccatttcatc cgtctgttct ggcagaaaaa
    24361 taccagcgaa actatcgtcc atttttgctg ggagcacccg ggacacaatc gttaagccaa
    24421 tatatgatga tgcgttctgc aggagatgct atgaaaaaca agaaagtcgt ctttattatt
    24481 tctcctcagt ggttcgttaa aaatggtgta aaaactgatt actttaatac gtattattct
    24541 gaacttcaaa catacgactg gttgttttca atgaagaaag taacgcctgc tgatcgctat
    24601 ttagctagaa gattattaac tttctcaaaa gtaaaagaga atgatacgtt gacggcgatt
    24661 ctgcaaacga tcaaaaaagg gaagctacct ttacctgaat ctttgaatca gctgcgcagc
    24721 caatggaaca tgctgaagag agaggatgaa gtattcagca atattgggtt gaaagatcga
    24781 cagcaaaaaa tcgatcatga gtcaaaacga ttaccaaaac aataccaaga aacagaatta
    24841 tccattttag caaatcaaat tggagaacga gaaacgacga ataatccatt cgggttgaaa
    24901 aacgattttt atacgcatcg tatccgagcc cacgagccag agctcaaaca gtcacaaaaa
    24961 aattgggatt atcgtttttc gccagaattt tctgattttc agctggtctt ggatcaatta
    25021 gcaaaaaacc ataacgaagt attgttcatc atcccaccgg ttaacgagaa atggagcgat
    25081 tataccggac tatctcaaga aatgttacaa ggatttgcca aaaagatcaa atttcaatta
    25141 aatagccaag gattcaatcg aatagctgat tttgtcaatc aagcgggtac caactatttt
    25201 atggaagaca cgatccatct aggctggaaa ggctggttgg ctgcagatca acagatcaga
    25261 ccgtttttag aagaaaatca tattactgct agtaagtatc atcttgacga tgcttttttt
    25321 agcaaatctt ggcaacatca aataccagat aaactacaat taaaataaag aaaacaggcg
    25381 agtgttgctc gcttgttttc tttatttttg aatattctga ataaaagata aaaataccaa
    25441 gtttgcctga acaaaaatca taagcaatgg tataatcaca atatgaatat agaaatgggg
    25501 gaaaacattt ggatacaaaa gaagaacagc tacttattga tttaacttct gcaaaaggag
    25561 tacctggaaa tgaagaagag gtaagagaag tattcagaga atatgccaaa ccattcgctg
    25621 atgatatttt ttatgacggt ttaggcagtg tgattgccaa acatggtgct ggcggaggcc
    25681 ccaaggtctt tatttctgga catatggacg aagttggttt tatggtaaca aaaataaccg
    25741 aaaaaggatt cttggaattc caaactcttg gaggttggtg ggggcaagtc atgcttgctc
    25801 agcaagttga aatcaaaaca cgtgaaggga aaatcgtcca cggtgtgatt ggttccaaac
    25861 caccacatgt tttaacacct caagtaagaa ataaacctta tgaaataaag gatatgttca
    25921 ttgatattgg cgcatcaagc caagaagaag ccaaagagtg gggcatacgt cctggagata
    25981 tggtcactcc atatattgaa tacaaacgaa tgaatggcag taaatattta ctggcaaaag
    26041 catgggataa tcgtatcggg acagctgttt ctctgcgagt attagagaat ctatctaaag
    26101 aagcacaccc aaatgtttta ttcgcaggaa gcgatgtgca agaagaagta ggcttgcgag
    26161 gtgctcgaac aagtacacac cttgtaaatc ccgatattgc ttttgcttta gatactggta
    26221 cagcaggaga cactccagga atgacaccaa aagaagcaga ttcgatttta ggaaaaggcc
    26281 cacagatttt gatttttgat gcatctatga tcccgcataa aaaattattg aattttgtta
    26341 ttgatgtagc agaggaaatg gatattccat tccaatatac agtgatttct ggcggaggaa
    26401 ctgatgcagg acaaatgcat ttgacaagag atggtgttcc gtctgtagcc attaccgttc
    26461 ctgttcgtta tctgcattcc catacatcag tgattcatga agatgattat ttgaatacag
    26521 tgaaactagt cacagaatta gtgaaacggc ttgatagtga aacagttgaa aaaattcgga
    26581 gttatgaata atgaataata gaatacctat ttctgaatat cggaaaaaag cacgagtttc
    26641 tttggaaaat caatggggaa tcaatacttg gcttgttttc ttatctataa tatttacgag
    26701 cgtcatccag aacttttttg acggtttcag tccttttccg gaagaaagca tgcagcagca
    26761 cttattgagt tttctagtcg ataatttact tttattcgca ttcaactatg cattgtttta
    26821 cattgcgcta gtagttgtac ggggtggcag accaaaaagt aaccttttat tttcagtttt
    26881 ccaaagaaag tattttggtt cgatacttgg catcaatttg atcaattcag tcatcaactg
    26941 gctactcaac agtttaatct ttctgcccat gtttttaatc agcggagtga gtacttatat
    27001 gcagctgctt ttcagttcaa atgcggtacc tatgggtgtc atgccagaag attttcttga
    27061 tatcagcttt gttttatcga tgatagggat gatttttgtt tcagcactca tcatgagtat
    27121 tgttggcggt ttattccaat ttgctgcatg gacaaagttc gactatcctg atttgtcggt
    27181 tattcaatgt ttgaaatatg gctggttttt attgaaagat agaattggta cgtatatttt
    27241 gttgcagctg tcttttatcg gctggtattt gctaggagca atcgctttgt tcatcggtct
    27301 tttgtgggtc attgtttatg tgaatgtgtc agttgcagaa ttttatgaac aagcacggat
    27361 tgaaaaagga ggtccagacg cctattttac ttagcatgta agcagtttat tcatcaaaaa
    27421 aataactttt gtggtaggat aaatctttga gggggaatgt acgatgaaat taactgtttt
    27481 aggatgctta ggagcgtatc cttataaaaa tcaagggaca acaggttatt tgcttcaatc
    27541 tgacgggttc aatttgttga tcgatgcagg tagtgctacg ctgatcaaat tacaagaaca
    27601 ccttgatcca cttgatctag atgctgtgat ccttagccat tatcatcatg atcatattgc
    27661 tgaccttggt gtattacagt attactggca acttcatcct aagagaaatc cggagacatt
    27721 gctttcaatt tatggacaca caaaagattc cattcacttt gaagaattga cgttggaaaa
    27781 tgtctcaaaa ggacatcctt attttgaagc tgaagaatta aaaattggtc cttttctgat
    27841 tacttttatg gaaacaatcc atccagtagt atgttacgcc atgcgtatcg tagaagaaac
    27901 aacggggaaa gtattcgtat ttacaggtga ttcaggttat ttggaaagtt tcgctgattt
    27961 tgcgaaagat gctgatttgt ttctagcaga tacctatctt tttgaaggaa atgaacatca
    28021 tcatgcccat ttcacttcaa aagaagctgg agaacttgcg aaatcagcta acgtaaagaa
    28081 acttgtactt acacacctac ctcagtttgg tgatttagaa caattgaaaa atgaagcgca
    28141 aaaggcagca ggcaaaggga ttgatataga acttgctgca gttgacaaag tgtttaacat
    28201 ttaggaggaa gaacatgatt tttgtaccaa atgaaaacca cgacccaaga atcaatttag
    28261 caattgaaca atatttgctg caagaaatgc ctttagatga acctattttg ctcttttata
    28321 tcaatgaacc ttcaatcatc atcggcagaa accaaaatac gcttgaagaa atcaatcgag
    28381 attatgtgga agaaaatggc attcatgttg tccgtcgctt aagtggaggc ggagctgtct
    28441 accatgattt tggaaacctg aactttagtt ttatcatgcc tgatgatggg gattcattcc
    28501 gtgactttgc gaaagtaaca cagccaatca tccaagcact tcatgaattg ggtgttgctg
    28561 gggcggaatt gaaaggccga aatgatctag tcattgatgg catgaaattt tctggcaacg
    28621 cgatgtatgc taccaacggt cggatgtttg ctcatggaac aattatgttc gactctgata
    28681 tcaatgaagt agtcaatgca ttaaaagtaa aaaaagacaa aatcgaatca aaaggaatca
    28741 aatcgatccg ttctcgtgta acgaatatca aaccatttct tccagaagaa aagcaaaaaa
    28801 tgacaacaga agaatttcgt caagaaatct tgttgaaaat tttcggcgta gagtcagtag
    28861 aagaagtaaa aacgtacgaa ttgactgatg aagattggaa aaaaatcaat cgaatctctg
    28921 aggaatatta tcgaaattgg gattggaatt acgggaagtc tcccgatttc aatttctctc
    28981 gccaaaaacg tttttctatc ggttcgattg aagtccattt gaatgtctca gaaggaatga
    29041 tcaaagaagc aaaaatcttt ggcgatttct tcggactagg agatatctct gacgtggaaa
    29101 aaaaattggt tggacagaaa tatgataaag catctctatc aaaaatagtc agccaaattg
    29161 atgtgaagaa atattttggt gcgatagaac cagaagaatt gcttacctta ctttattgaa
    29221 aataaaaaat agcggaatcc acatatatgg attccgctat tttttataca ggattcgggt
    29281 aatttcgaga gagttgaaca gtgatccgat caatgaaagt atctttttcc tgcgttagtg
    29341 ttcctgtgta acgatccgct acgtctttca tcatcattaa gtcaaaacgt tcctgatcat
    29401 ctaattcttc tgtgatgcct cgactaaata ggtttgtatc gtaatgtgca ctatgttcgc
    29461 aacaaatgac gaaatgttcc tcttgttcgt gtatcgacaa ataaattttt cgatacagtg
    29521 gattttcgat ggcatgagtt tctctaaagg aatgctctaa agcgtggatc aaaagttgag
    29581 ccagatcgtc atcgttgata ggaagttctt cgggaagatc tgcttggaag gttatctgaa
    29641 tattccgttt ggcagctaac ttctggtaac gcgctaagat aagatttgtc acctggtggt
    29701 tgcaaaaaga acacagcatt tcgaaatttt ttgcttcttc taatatcttt tccacatagt
    29761 ttttagcttt tgtttggtcc gtatgcatta aatcttgcag attctccatc tcttggacaa
    29821 attccaggcg aagtgtattt acttcttgga tatgctgtcg aagctgctca taatgttgct
    29881 ccaacaaatc atttttgatt tttaagaaat ctacttgtct ccgatctttg tcggtattta
    29941 agatgtgatc gattagattg tagctgataa taacgattag aatgataata tataaaatcg
    30001 tagaccaatt gatcgttgtt tgatagatac ctaatgccgt ttggatgtaa atcaagcaat
    30061 ggataatggc taccaagacg atccaaggtg tacaagctac ataaaaacgg ttattcttat
    30121 atgcttcgcc aatgcatgca agtgaagtga ctaaagtgct tataacacta gcaatattag
    30181 caatcggcat taattcagga agttctgcag gagtaaaagt tgcataaagc agaacgccga
    30241 taaaaatcaa aagttggaac gtacaaaaaa ttccgtacca ttttttatag taaaacattc
    30301 ggctccaata gtagcagatg atgaaagctg acgttaagat aataaataaa tgagataaaa
    30361 aagaatgaac gaatggttca aaaagaatac cacttaaaat atcttctgat acagcttcaa
    30421 atcctaatgc caaagcaaag caacttaaga gaataagtga gtattcaaga cgtttttgtt
    30481 tatacatcaa gtagatcgta tagaaaaagg tccctatagc gataaaaaca gcgattatca
    30541 tcgtcaatac ttgcggcact gattgtgaat aaataaaact aatcaatggt ccagttgttc
    30601 ctagataaac tttcggtggc agtccagcat agttctgata aggagatgag actgtaaggg
    30661 tcaatttttt tccgatataa tttgctggta aatctatcac agccaatgag agtccgggat
    30721 tgctttcttc ctgataatct ttccgttggt ataaaatctt ttcgcctaga tatacagtaa
    30781 tccattgatg gtttcctttg attagtaaat ttgcttcgtc caacggtttt tcaagtgtcg
    30841 tttccataat gaatgtttcg tctttatcca cggtaggaat cgtacgagtg tacttgctgt
    30901 caaacttaaa atcaggatca tcttttgtat agaaaaccca cgaatctttt atttctttca
    30961 tttgattgct gtccgcagga gaaaaaataa aataaaagaa ataagcaatt cctgttaaaa
    31021 ataacaatga taatataatg attatgctcc actttatacg taaataaaaa gattcttttt
    31081 tattatatat gttattaaaa aaagacataa aggtattcct ttctaaaact tgtaatgtta
    31141 gggtaaacgt tttttaaact aacgtcaatt atttataata aaaaatgaga taaagatgag
    31201 gaaatagaat tagaaacata taattattaa agggaaacat ttttacagct ataatttgaa
    31261 aaaaatttat cgttattata tgattaagaa ggattgaata aagaaagaag ttgaaaacga
    31321 tacctgatta caaaaaaata acttttataa gttctataac gttgattata tttttaattc
    31381 ttattcttct cgtcgtcttg gttccccgaa tttttggttt gactccttat attattgagg
    31441 acaaagaaat gagtccatat tatgcacaag gaaatctgat atatgtacga ccgaaaaata
    31501 caaatgagat tttagtcgga gatattatta catattatga aaattcagga aaaaaatctc
    31561 gacgcgtcgc gtaatagccg ttgaaaacaa ccacgaagat ttttatacaa aagctgatac
    31621 aaagacttat attgagcctg gagtagtaaa aaagagaaat attatcggta gtcccatctt
    31681 tcagattcca tatattggac tagtcctttc aaaaacggca tttgcatgga ttcaggggtt
    31741 attctttacg attgcttttt gcctaacagg aatcacagca tggaatacct ttgtagagtt
    31801 gaaaaagaag cggagacgaa cacagaaatt tttgtaagaa tgttaaaaac ataaatgtat
    31861 tccagagatc caacgactct tctgaacatg aaacacgaga aaacactcat gcagcttgta
    31921 gatttagtca gttgttcgta taatgtgtga agaggaggag tagtatgcgc aatgaaatga
    31981 tgcatcgtcc tgtagtcaaa gaagaactat tggattttat gcgtacccag caaaaaaaat
    32041 taccgggtga gctaggcaaa ctggaagaag aagctcatgt agcagaagta ccagttattc
    32101 cccatgaaac agttgtattt ttgaaatttt tacttggaca gcttcagcct gaacgaatct
    32161 tggagatcgg agcagctatc ggtttttcat caagtgtcat ggccacgaca atcggagaaa
    32221 acgctcatgt tacgacaatt gatcgtttcg atgtcatgat cgagaaagct aaaaaaaact
    32281 atgaacgtct aggactgaca gataaagtga ctttgcttga aggtcaagca gcagatatct
    32341 tgccggagtt gtctggtccg tatgacttta tttttatgga tagtgcaaaa tcaaaatata
    32401 ttgaattttt acctgaatgc ttacgtttgt taagaaaagg tggcgttttg atggtagatg
    32461 acatcttcca aggaggaacg atcttgctcc cagatgaaga gattccccgt ggcaaacgag
    32521 ctatccatcg taaattaaat gaatttcttc gtgttgtgat ggatcatccc gatttgacat
    32581 cgacaatctt gccattaggt gatggtgtga tcttaatgac aaaggaatca gaaacaatcg
    32641 agttgtgata catgattatg gcagcctgta gataatcgat tgaatcgagg gctcaattcg
    32701 aacttgatgt aaaagtttct atatttgtaa gtcaattaaa ggatacagga aatctaaata
    32761 aacaggagta gcatgaagtc aatgtagctc ctgttttcaa ttgataacct tagaacagct
    32821 gaaggagaat gtctcgtttt aaaagattga tttagtagtt gggccaaaat aattcattat
    32881 ttgctaatta aataactatt tcgtattgac agaagcaatt taattatcta taattattga
    32941 tgttccgctg attcttgtca tagtagctca gctggataga gcatccgcct tctaagcgga
    33001 cggtcggggg ttcgaatccc tcctgtgacg ctaaaaaacc gttagaaact gaggtttcta
    33061 acggtttttc tttgtttttt gtgccaatat tgtttgtttt caaaggcaaa taaaataaaa
    33121 aaacatcctt atcgagtaca attataagga gcaaaaaaat aaaactcatc agaaaagagg
    33181 aagaagatat gacgatacta tctagaattt tagtgactct agttgcaatt gaattttttt
    33241 atatcatgta tatcgaaacg gtgagaactg attctgacac gacaagtagg gtttttaaaa
    33301 tgagtaaaga agagttaagc agaaaatctg ttcaaacatt gtttaagaac caaggagtgt
    33361 acaatggact attaggagtg ggactacttt atggagctta tctttcttcc gcatctaaag
    33421 aaatcacaag tatgctatta atatctattt tttttgttgc attatatggc agtttgacta
    33481 gcgatcgttg gattttactc aaacaaggtg ggctagctct gttggcattg ctttctattt
    33541 tcttataaat atagaaaaaa taagaattta tctaacagga aactttgtga aaaatattac
    33601 tttatctcat gaaatatgat attctaatga atggtatttt agaaaatttc atgaaaaagg
    33661 agttagatag gtgttcaaaa attatgtttc ctcactaaaa aaagaatttt caggttatga
    33721 tctttcaaga tttcagaaag atcttttggc tgggatcaca gtagctgctg tagcattgcc
    33781 tttagccttg gcttttgggg tatcaagcgg agctgatgcg gccgcaggtt tgattacggc
    33841 tatcattgcc ggtatcgtta ttggcggttt atcaggcggt ttttatcaaa tatcaggtcc
    33901 gacaggtgcg atggcagcta tcttgatgtc tattgcggca acaaagggaa tgcaaggtgt
    33961 attgcttgct acttttttag cgggtctatt tttacttgca gcaggacttt tccgattagg
    34021 aacactgact tcttttatcc cagcacccgt aattacgggg tttacttcag gtattgctat
    34081 cattattgca ttaggtcaga tcgataattt atttggtgtt cactcggaag gtacaaacgt
    34141 tattgaaaaa ctaagtagtt atcaaacgat aggatttccg ataaacacag cctctttgat
    34201 catgggaggg ctagtcattt tagggatgtt tatctatcca aaaaaatggg cgaaacgaat
    34261 gcctagttcg ctgctggcta tcattttggc aacagcagtt atgctattgt tccatttacc
    34321 agtagcaact gtgggtaaga ttccacaaac attgatcagt agtaatcgtt tgaatatggg
    34381 agattttagc ttttctgcct tgcaacaagt ggcagttcca gctattagca ttgctttatt
    34441 aggaatgatt gaaagcttgc tatgtggtgc ttcggctggc cgaatggcaa ataagccctt
    34501 agatagcaat caagaattaa tggcgcaagg aattggaaat ctgttgcttc ctttctttgg
    34561 cgggattcct gctactgcgg ccatcgcccg tacaagcgtg gccatcaaat ctggtgcaca
    34621 aactagatta gctggtatga tacatgcagt tgtcttactg ctctccatgc ttgtttttgc
    34681 gccagtgatg tcacagattc caatgccagc acttgcaggt gttttgattg tgacggcttg
    34741 gcgtatgaat gaatgggaaa cgatcaagga gctatttact aataaatatt ggtcagcagt
    34801 tctattgttt atccttacga tgatttgtac agttatcttt gatttaagta ttgctatcgt
    34861 gatcggggtg atcggcggct gtgtattttt tgttgtcaaa agcgctgcta tcacgatttc
    34921 attagaaacg atcgattggc aaagaatggg gctgccagaa acggaaaagc tgaataactg
    34981 gacagtagtg tatattagtg gtccactgtt ttttatgtct tcagaaaaac tcaaaaataa
    35041 attggaagaa ctaaatcatc acgatggtgt cattttctcc atgcgaggcg taccaagtat
    35101 tgatttgacg gcacgtgcag tttttgatga atttcaagaa aaagcagagc aaagaggaca
    35161 aacgattatc tatacttcct tgcagccaga agtagaaaaa caactttctc acttgtggga
    35221 acatacattc acgcatcaac atcaaacagt tgctcatgcg ttgacagctt tacatgcgaa
    35281 atatactgca attagccatt aaaacaatga aaaagaacag acaagactgt aaactgaaga
    35341 ttttatggtc ttgtctgttc tttttaatat tgctgtaaaa gtgaagctag tacttgccag
    35401 tcttgatgga taacaggtac aagggagcga ttgtagaaaa tagctgctgg atgaaaaagt
    35461 ggaagaattc gatagttttt ttctgaccat tcgtaggcat ccttttgttc tgttaattgt
    35521 aagacaggcc ctgtatagag ttgaccatga tttttggtaa ccgtatattc tttgccaagt
    35581 aaacgttgaa gaccaatgtt tccaagtgtc gcaattaatt ttggctggac cttcatcagt
    35641 tcgtaatcca atattggtgc atgcgcaaat acttcggaac gagtaggtgt ccgatttgga
    35701 taaacagttt ctgactgttg tgtccgttta ttgatccgat gagtcacgcg atacgggcgg
    35761 ctccttacag cactggtgat atacacctct tctcgtgtta agccagttga agaaagtgcc
    35821 tgcatcaatt cttttccagc ttgaccgcta aaaggaacat gcgtatcaat ctctgttttc
    35881 cctggggctt ctcccacgag catcagttta ggatgaagag gtccttgccc agcgataaat
    35941 ccttctacag gacggccgtt cattcttaaa gaagcttctt gtatcagttc ttttgggtat
    36001 tccatttatg ctcctccttt ttcttttatt ataagaataa aagaaacaca atgcgactat
    36061 tttatttggt agaactgtga ggatagaaaa gcagaagtat tgcttgaatt tttgttttac
    36121 tttttgattt taaatttagt tgactaacgg ttgctgttct ggtaagctag tactgttgta
    36181 attgtggact ccacagctac aaccgctcag aacaagtaat aagcacagca tatgctgtca
    36241 cttcggatgg cgagtctaag tatatcagaa ggaggtgctt tatagcatgt acgcaattat
    36301 taaaactggt ggtaaacaag taaaagttga agtgggacaa gcaatctatg ttgaaaaact
    36361 tgacgttgaa gcaggagaaa aagttgtttt tgacgaagtt atcttagtag gcggagaatc
    36421 tacaaaagta ggcgctccaa ctgttgcagg tgcaactgtt gaaggtactg ttgaaaaaca
    36481 cggcaaacaa aagaaagtcg ttactttcaa atacaaacct aaaaaacatt cacaccgtaa
    36541 acaaggtcat cgtcaaccat atacaaaagt ggttatcgac gcgatcaacg cttaatagcg
    36601 tttgagaaag aaggcctata tatgatcaaa ggaacattta agcgaaatga cgcaggtcag
    36661 atcgtttcat tcactttgac cgggcatgct gatgctggtc cttatggaag tgatatcgtt
    36721 tgtgcgggtg tttctgcgtt agcgatcagc actgtcaacg ggattgcctc attagccggt
    36781 tttgagccga ttgttgaaat gaatgaagaa gaaggcggtt atctttatac cgaagtcact
    36841 tcaggaatga cacaagaaca aaacaatatt gctgagattc tattagaaaa tcttttatta
    36901 ggattacaat cgatagaagc tgaaaactca gaatatatcc aaattaaaac aattaatgat
    36961 aaataggagg tgcagaccat gttgttatct atgaatctac aattatttgc ccacaaaaaa
    37021 ggtggcggtt ctacatctaa cggccgtgac tcagaatcaa aacgtctagg tgctaaacgt
    37081 gctgatggac aaacagttac aggtggatca attctttacc gtcaacgcgg tacaaaaatc
    37141 tatccaggtg taaacgtggg tatcggtgga gacgacactt tatttgcaaa agttgatggc
    37201 gtagtacgtt tcgaacgtaa aggccgtaac aaaaaacaag tgtctgttta tccagtagct
    37261 caagaagctt aattgaatct tatgattaaa gaggttgtga caagactttt gtcacagcct
    37321 tttttgttta taaaaataaa agttgtagtg gaggctacga agtttacttt gtctttttac
    37381 acttaatatt tttttgccaa tttggaatca ggatgttttg ataactatcg ttttcaattg
    37441 atggaagcta tattgtttgc tatcagtgaa ataattatat ggtaaacagt gttctttttc
    37501 ttgtttccat tataaaaatc gtatcgggat ttttcgttct tttaaattga acatttctat
    37561 ctatccgcgt gttatcaaca aaacaaaatc aggtaaatag ttgacgaaac agaaataaat
    37621 acatagtatt atgttttgaa gcctaagtac aatagtctat agaaaaggag cgataccatt
    37681 ggcaaaacgc attgaccgaa tttatatgta tgtcaaagaa aaaaccgctc atttaacaaa
    37741 ggcagaatat gatcaaggag taacaacaca ggaaattgct gaagtgttag ggattcagcg
    37801 aacaaatagc agtaaagatc tgaatcagct agtgcgcgaa ggaaaactga taaagacaga
    37861 cggacgacca gtacgttaca tatatcaaac actcgttacg cacgtaaagc cagttacaaa
    37921 acatgtagtc agttacaaag aaacgccggt ctctgaagaa aaaaatacac tccaaataga
    37981 tactaaggat atctttgcaa aaatcattgg agctaacggc agcatgaaaa attcagtgga
    38041 acaggcaaaa gctgcgattt tgtatccgcc aagaggatta aattgtttga ttacaggacc
    38101 taccggatca ggaaagactt attttgcaca cgcgatgtat cattttgcaa aagcgcatca
    38161 tgtaatagat gaggaaacgg aactgattgt tttcaattgt gctgattatg ccaataaccc
    38221 tgagcttttg atgagtcatt tatttgggta cgcaaaaggt gcttttacag gcgcagatga
    38281 agaaaaaatg gggattatcg atcaagcgga tggcggtatg ctgtttttgg atgagattca
    38341 ccggttgccg cctgaaggtc aagaaatgat cttttacttt atggatcatg gaacttacag
    38401 tcgtcttggg gaaacaacga aatcccatga agcaaatgta cggatcgtag gtgcgaccac
    38461 agaagaccca ggttcttctc tattagaaac ttttgttcgg cgtatcccaa tcaatatcaa
    38521 acttccggct tttgaaaaac gtcctgcaaa tgagaaagtc gatttagtaa aaatcatgat
    38581 tgcccacgaa gcaaaccgca cgcagcgaaa gatttctttg acagaagatg tagcaaaagc
    38641 attgatcggc agtgtgacct atggaaatat aggacaattg aagtcaaata tccagttagt
    38701 atgtgcacga ggatttctaa atcatatgca atcacccgaa atctcgatca caatcgatga
    38761 tttgacagaa gggattcgta gcggattgat tcaattagca agcaaccgca gtgcaatggc
    38821 agagctttct aaaatattgg agccaaagat aactgtagca ccaaatgata cgatcatgaa
    38881 aatccaatcg gactcctatg agttacctta taatctatat gatatcatcg gagataaagc
    38941 agctttactg aagtcggatg gtttggatca agaagcaatc aatcatttca tttctacaga
    39001 tatcaacatc catctgaagt ctttctataa agaccatggt ttttctttta atgcagacag
    39061 caaattagca gaatttgtag atccgaaaat catagaagtg accaatcaga tttatgtaat
    39121 ggtcaaaaga tctttaccat atgagtttca gcaaaacttt atttatgcta tgagcttgca
    39181 tatcagttct tttttgaaaa aaatcaactt aggagaagaa cggcatacga atgataacat
    39241 ccgtgagatg gcttttgatt ttccagatga atatgctgtt gcgaaagaag tgcgtagtta
    39301 tatcgaaacg tattttcaag tggagattcc agaaagtgaa gactattact tgaccgttct
    39361 actcgtctcg ttacgggcaa atcaagcaag cggcagaatc ggtgtagtcg ttgcagctca
    39421 cggaaacagc acagcaagca gtatggtcca agttgtccaa cagttgttag atgcagagaa
    39481 cgtacgagca gtagacatgc ctttggatat ggatccgaaa acagcattgg accgaattga
    39541 acgcaatgta caagaagtag atgaaggaag cggggtcata ctcttagtag atatggggtc
    39601 ccttgcgtcg tttaattcgc aaattcaaag agacactgga attccagtac gtactgttga
    39661 tatggtaaca acttctctag ttttggaaac tgtacgaaag gtctcagtat tagggacaga
    39721 tctagatatg ttgcatgaat cattgaaaaa ttttcgaggt tacgcagaaa tatcttctgt
    39781 agaatctgtt caggaagcaa aacctattca taagaaaaaa gctgttttgg ctatctgcgc
    39841 atctggcgaa ggtacagcac aaagaatcaa agaattgatc gaacgagcgg taaacaagcg
    39901 tcaagaaaca gaattagaag ttctagcttt gtcgattgtg gaagtgaaag aagaactgcc
    39961 agttatccaa aaagaatatc agatcatagc tactactggg attactgatt tgaatatcca
    40021 agccccattt attcctttag aacgttttat tgatcaaaat atcgaagtca ttttggatca
    40081 gttgctgatg gaatcagaat tggaggaaac tgaattcatc tccctagatg aagagtcagc
    40141 taaaaatacg tgtgttgagt ttatctctga taacttcatc tttattaatg gtagtaagtt
    40201 aatcgatcca atgtggcaat tttctacaca aatctctcag acgacgggaa tcggcgatga
    40261 agaatatgga ttcaaaatca acctagtgat gcacacagca ggaatgatcg aacgtattat
    40321 tcggaatgaa ccattgactg tggaagaaaa cgaattgaca aatacaacga atgatcctct
    40381 ttatagccaa ttggcagcct cagttgttct gttggaagat cagataaaag taaaagtgcc
    40441 gatagaagaa atgtattatc tgctacgtct agtccataat cagttagata aaaaagaata
    40501 cactgtgcca tgaatacaca aaaacgatac acttttatag tgtatcgttt ttgtgtattg
    40561 gttaagaatc ctttaaattc aattagtgta tcgtaaaaaa gttggcacgc tttttgctta
    40621 tagtagtgtg tgctaggagg tggttgttca cacagaaatg ataggggttt ctgtgaagta
    40681 gtgaggaaga aggagttttg cagatggata ttcgattggc acgtatagat gatcgtttaa
    40741 tccacgggca agtcgcaaca gtttggtcga agcaaacagg tgttgaacgt atcattgtga
    40801 tcagcgatgt agtcgctcaa gataaattga gaaagtttct actaaaagaa gctgcaccgc
    40861 cggggatcac agcaaatgta ataaccgtgc agaaaatgat tcaaatctat catagtggat
    40921 tattatcacg agcaaaagtg atgcttttat tcaccaatcc ccaagatgtt gaagcggttg
    40981 tccgctgcgg tgtacagttg aagtcgttga atatcggcgg tatgagtttt actgaaggca
    41041 aatcaatgat taccaatttt gtttcagtaa atcaacaaga tattgattcc tttcgttact
    41101 tgaacagtca agaagtggag ttagagattc gaaaagtacc tgctgacagg aaaatccttt
    41161 tgatggaact actagcgaaa atcaaaaagg tgtagcaaga atggttgttg gaaaaggtca
    41221 aataattctg ttgtaatggt tagcaattat taatattttt gaatttgtta caacaaaagt
    41281 aaataggagg tataaatatg gtagggatta tcctagcaag tcatggacaa tttgctgaag
    41341 gaatcttaca atccggttca atgatctttg gcgaacaaga aaacgtcaaa gccgtgattt
    41401 tgaaaccaag tgaaggtcca gatgacttga gagccaaatt ggaagaagcg gttgcttctt
    41461 ttgataatca agacgaagtg ttattcttag ttgatttatg gggcggaaca ccgttcaatc
    41521 aatcaaacac attattcgaa gagcacaaag ataaatgggc aatcgtcagc ggtttgaatt
    41581 taccaatgtt gattgaagcc tacgcatcac gcttttcaat ggaatctgct catgaaattg
    41641 cagcacacat cattgaaaca gcaaaagacg gtgtaaaagt aaaaccggaa gaattagaac
    41701 cggcagaagc accgaaagca gcagttgaag acgcacaacc taaaggagca cttccagaag
    41761 gaactgtggt cggagacgga aaaatcaaat atgtattagc acgtgttgac tcacgtctgt
    41821 tacatggaca agttgcgaca gcttggacaa aagcagtgca accaaaccgt attatcgttg
    41881 tttcagatgc agtatcaaag gatgatctgc gtaagagact aatcgaacaa gctgctcctc
    41941 caggtgtaaa agcaaatgtt attcctatta gcaaaatgat cgaagtagca aaagaccctc
    42001 gttttggaaa taccaaagca ttgcttttat ttgaaaatcc tgaagacgta ttgacagctg
    42061 ttgaaggtgg cgtagacatc aaagaattaa atgttgggtc aatggcgcat tcggtcggta
    42121 aagtcgttgt aagtaaagta ttgtctatgg gacaagaaga cgttgaagcg tttgagaagc
    42181 ttgaacaaaa aggtgtgaaa ttcgatgtac gtaaagtacc taatgattca cgcgataaca
    42241 tggatgatat tttgaaaaaa gcaaaagctg aattagcaaa agcgtaataa ataatattaa
    42301 aatttatagg aggcttatca tgtctattat ttcaataatt ttagtcgtat tagttgcctt
    42361 tctagctggt atggaaggta ttcttgatga atttcaattt catcaaccac tagtggcatg
    42421 tacattgatc ggtttagtta ccggtaacct cgaagctggt atcgtacttg gcggtacgct
    42481 tcagatgatc gctttaggtt gggcaaatat cggtgccgct gtagcaccag atgctgcatt
    42541 agcatcagta gcatcagcaa ttattttagt tttaggtgga caaggtgtta aaggtgttcc
    42601 ttctgcgatc gcgattgccg ttcctttggc tgttgcaggg ttgttcttaa caatgatcgt
    42661 acgtacaatc gctgttccaa tcgttcattt gatggatgcg gctgctgaag aaggaaacat
    42721 ccgtaaggtt gaaatgtggc acatcattgc tgtatgttta caaggggttc gtatcgcggt
    42781 cccagcagca gcgttgctat ttatcccagc acaaactgtg caatcattcc ttgaatcaat
    42841 gccagcatgg ttaacagatg gtatggctat cggtggggga atggtcgtag ctgtaggtta
    42901 cgctttggta atcaacatga tggctacaaa agaagtatgg ccattcttcg ttattggttt
    42961 cgttgtagct gcaatttctc aattaacatt gattgctcta ggtgctttag gtgttgcatt
    43021 agctcttatt tacttgaacc tttctaaaat gggtggttca tcaaataatg gaggcggaag
    43081 caactctggt gaccctttag gcgacatttt gaatgactat taatcttgaa ggaggaaaat
    43141 aaaatggcag aagaaaaaat caaattatca aaaaaagatc gtttagctgt tgcgtggcgc
    43201 tctaccttca ttcaaggttc atggaactac gaacgtatgc aaaacggtgg ctgggcattt
    43261 tctatgattc cagcaatcaa aaaattatac aaaacaaaag aagaacgttc tgcagcatta
    43321 aaacgtcact tggaattctt taatacacac ccatacattg cttctccaat tttaggggtt
    43381 acattggctc tggaagaaga acgtgcgaat ggcgcgcctg ttgatgatgt agcgattcaa
    43441 ggggttaaag taggtatgat gggtccttta gctggtgttg gtgacccagt gttctggttt
    43501 acagtacgtc caatgctagg tgcactaggt gcttcattag ctatgggagg aaatatcctt
    43561 ggaccaatca tcttcttctt aggttggaac attattcgtt gggcattcat gtggtataca
    43621 caagaatttg gctacaaagc tggttctaaa attactgatg acctttctgg cggattgtta
    43681 caagacgtaa caaaaggtgc ttcaatttta gggatgttcg tattagctgc ccttgtacaa
    43741 agatgggtat caatcaaatt cttaccagtt gtatcaactg taaaattaga taaaggtgct
    43801 tacatcgaat gggataaatt gccttcaggt ggtgaaggaa tcaagagtgc atttgaacaa
    43861 gttaataatg gcttagccct ttctcctgaa aaggtaacaa cattacaaga caacttagac
    43921 caattgattc ctggtttagc tgcattagga ttaacattgt tatgtatgtg gttgcttaag
    43981 aaaaaggttt ctccaatcgt tatcatcctt ggtttattcg tagtcggtgt tgttggacac
    44041 gtcatcggac ttttataatc cataaaaata tagctcgggc gttgtcccga gcttttttta
    44101 tatagtatac tgttcataaa caaatatctg gagggagaaa atggttcaat caatcaatac
    44161 aaaagtagac ttggtaattg atgctacttc gtttacgggt ctgacagatt acgggcaaat
    44221 catgataggt gataatggtt ttgagtttta ccatgcgcga gatagacgca aatatatcca
    44281 aattccatgg gaagaagtgg attatgtact agcatcagtc atgctaaagg gaaaatggat
    44341 tcctcgatat gccattcaaa cgaaaaaaaa tggtacatac acattttctt ccaaagaacc
    44401 taaaaaagtg cttcgtgcga tcagagaata tgtagatgaa gatagaatgg tacaatcatt
    44461 gagtttcttt gatgtagtga aacgtggtat tcgtagtatg tttaaacgca agtaaaaaat
    44521 aaagaaaact tctaagagga ggttttcttt atttttttac tttgcagaag aaaatatgaa
    44581 atagcaaaac caattatttg tcccctttaa atcccatatg ctaaaattga ttagcaaaga
    44641 aggaggttat attatgagtt ggttatggac attgattgtt ggagccgtga tcggagcaat
    44701 tgcaggtgcg atcaccagta aaggaaattc gatgggctgt gtaacgaata tcattgctgg
    44761 actagtcggt tcaagtattg gacaagcgat tttcggcgaa tggggaccaa aagctgcagg
    44821 tatggcactt attccatcta ttttaggcgc agtgattttg gtagcagttg tctcattttt
    44881 ctttggaaaa cgatcataag tttttacaaa atagtaaact aaaaggagtt gcagcgagac
    44941 tgtgactcct ttttggcttt atttatttaa aataaaaaga agttttccgg aaatttttat
    45001 ttaactgtcg tgtacgaaga aaagtttgct ataatggaaa aaagcgtgca aagaaagagg
    45061 gagtttcatt atgatgctac gtgtagaaaa attgcgcaaa aaaatgcaag aagaaaattt
    45121 ggattcattt ttggttacca gtccgtacaa tcttcgttac ttaacgaact ttacaggtac
    45181 gacaggatta gctgttatta ctttggaaaa agcatttttt attactgatt ttcgttatac
    45241 cgagcaggcg gcagcgcagg ctcaagggtt tgaaattatt aaaaatgtcg gacctatttt
    45301 tgatgaggta gcaaacttag tacgcaaaga agaactgcac tcattagctt ttgaggaaac
    45361 aacagtctca tttttagaat attcggtttt agaagaaatc atcgatgcgg aattagtacc
    45421 tgtttcacag atgatcgaag aactcagaga agtgaaagat gaagaagaaa tcgctattat
    45481 cgaaaaagca tgtcatattg cggatatggc atatgatcac atcctgaaaa tgatccaacc
    45541 gggcatgact gagattgaag tcgcaaatca actagacttt tatatgcgtt ctcttggcgc
    45601 ttcaggtgtt tcttttgata cgatcgttgc tagcggattg cgttcggcta tgccgcatgg
    45661 tgtagcaagc aaaaaaatca tcgaacaagg tgacttgatc acattggact ttggttgcta
    45721 ttacgaaggg tacgtttccg atatgacacg tacattcgct attggtgatc ctggggaaaa
    45781 attaaaagaa atttatcaaa tagtcttaga tgctcaatta gcagttattg atgctgcaaa
    45841 accaggaatg actggtgtcc aattagatgc agttgctaga gattatattt caaaacatgg
    45901 ttacggggaa gcatttggac attctacagg acatgggatc ggtttagaga tccacgaagg
    45961 accaaacgtt tcttctcgtg cagaaaagca atttgtacca ggaaatgtga tcactgatga
    46021 accagggatc tacttaccag gaatcggtgg agtacgtatt gaggatgact tgttgatcac
    46081 agaagatgga aatcgtgtgt tgactcgttc accaaaagaa ttgatcatct tatagtttca
    46141 tcaatagaag aagtaagcgt aaaaatcagt gttcacagac tttgttatag aaaatcactc
    46201 gatttaatga tacactatag aagaactttt aagctgtaag agataataaa gttgggtgtt
    46261 ttctccaatt catagcagtt tgcagctgat cggcggcagt tttcttacca cgtgatcact
    46321 atgccgcatc atagagaatt cggatgtatc tcgtgcttag gcaaagggag gaataaataa
    46381 atggcagatg aaaaaaattt gattttaaac gcaaaccaag atttaggaga gatcgtgatc
    46441 gctcctgaag tcatcgaagt aattatcggg attgcagctt ctaaagtaga aggcgtatac
    46501 ggaatgcgcg gaacttttgc cagcaatgtc actgaattat taggacgtgc agctcacgga
    46561 aaaggtgttt acttagcaaa cgaggaagat ggcttgaaag tagatcttta ctgttatcta
    46621 gaatatggtg tttcagttcc taaagtagca atggaaatgc aggaacgggt cagccagcaa
    46681 gtccttttca tgacggatat cgatttatct gaagtcaata tccatgtagt agctgttgtt
    46741 cctgaaaaat tagaacaacc aacaatcgaa gaactttttg aagacgaaga ggaagagaat
    46801 gaataaagaa ttatcaagac acgagattcg ggaaatggca ttgcaggctt tatttccatt
    46861 agacttcaat gcagacctaa caaaagaaga tgcgattttc aacgcaatcg aattagacca
    46921 ccgcgacatg atcaacgaag atgaatcaga gtttgttcct gtttatcttg atacgctagt
    46981 tggcggtgtc tgtgcaaaaa aagatgaatt ggataaagtg attgaaaaac atttaaaaaa
    47041 caattggcac atcaatcgga tctcaaaaat ggatttggtg atcttgcgta tcgcgatttt
    47101 tgaaatgact tatgtgacag atgtaccagc ggctgttgct ttaaatgaag caatcgagtt
    47161 ggcaaaaaca tttagtgatg accgttcacg taaattcgtc aacggtgttt tatccaatgt
    47221 attgaaagaa ttagaagctg gggcgtaagt cctagctttt ttctatgaat catcataaac
    47281 ctgttataaa tcgggaaaat tctatggtag aatgaaaaca caaaaaatat ctgggagtgg
    47341 ttagatggca gaattgatta acggaaaaga attagctgaa aaaatgcagg cagagattgc
    47401 tgaaaaaatc caaaaattga aagataatgg tatccatccc ggattagtcg ttttattggt
    47461 aggggaaaat ccagccagcc aaatctatgt aagaaataaa gaacgtgtag caaaagaaat
    47521 cggaatccat tcattggtag agcgttatcc tgagacgatt tctgaagacg aactacttgc
    47581 agaaattgaa aaatacaatc aagatcctag ttttcatggt atattggtcc aattaccatt
    47641 gccaaagcat atagatgaag aaaagatcct gttagctatc cgccctgaaa aagacgtaga
    47701 cggttttcat ccgatgaatt taggcagact ccttgcgggt aaaccggaca agattccttg
    47761 tactccatac ggcatcatga aaatgtttga agcttattct atcgatccgg cgggtaaacg
    47821 agcagtagtt atcggacgaa gcaatatcgt cggtaaaccg atggctcagc tactcttgat
    47881 ggcagatgca actgttactg ttgctcattc taaaaccgaa aatctggctg aactggcaca
    47941 agaagcagat attttagtcg tagcaatagg acgtggacat tttgtcacaa aagaattcgt
    48001 aaaaccaggt gctgtagtga tagatgtagg gatgaatcgt gatgctaacg gtaagctgat
    48061 cggtgatgtg aaatttgatg aagtggagcc tattgcaggc tggatcactc ctgtgccaaa
    48121 gggtgtaggg ccaatgacta tcacgatgct gatgtatcaa accgtaaaat ctgccgaagc
    48181 ttttggagca ggtgaataga tggcacaaga atatttaact gtcactacat taacgaagta
    48241 tttgaaaaga aaatttgatg ccgatcctta tttagaacgc gtatatttaa caggcgaaat
    48301 atcgaatttc cgtttacgtg ccaatgccca tcaatatttc agcttaaaag atgaccatgc
    48361 aaaaatatct gctattatgt tcaaaggagc attccaaaaa ttacgattcc agccaaaaga
    48421 aggaatgaaa gtccttgtcg ttgggcgtat ttcattatat gaagcgggag gttcttatca
    48481 aatctatgta gagcacatgg agcctgatgg aataggtgct ttatatcaag aattggaaga
    48541 acgaaaagaa aagctgatga aggaaggcct ttttcaaggg cctaaaaaaa tgctgccacg
    48601 tttcccaaag cgaatcgcgg tccttaccag tccaagcgga gcggttatcc gcgatatcat
    48661 cacgacggtg aaaagaagat atccaattgt ccaattggtt ttgttcccaa cagttgtaca
    48721 aggaaaccaa gcagcggaca atgttgtcag gaatatccag agagtagaag agatgggcaa
    48781 tttcgataca atgatcatcg gtcgtggggg tggttcgatt gaggatttat ggccgtttaa
    48841 tgaagagcgg gttgctcggg cgatttatca agctaatacg ccggtgattt catctgtagg
    48901 acatgaaaca gataccacga tcgctgattt agtagcagat gttcgggctg ccactccaac
    48961 agcagctgca gaattagctg ttcctgtttt gtcagaagag ttgatgcgca ttcaagaacg
    49021 gcaagctaga ttgcagcagg cgtttgtacg ccagatccag cgcaaacaag aacggttcga
    49081 gcgtgctcga cgttcctatg tatttcggca gcctgaaaga ttgtatgaag gtcagaccat
    49141 caaattagac cagttgaaac agcgattata tcaagctaca cagcaaattt atcatgcgaa
    49201 agaaaaacaa acgatcgcat taagccacca attggaacaa gtggctccgc tttatcgagt
    49261 taaagcagct cgccaagaaa ctgcttatct agaaaaacgc ttagaagaga aaatccaaca
    49321 atatatgaaa caacagcagc agcgttttgc tcaagctgtt caatctcttg atttgttgag
    49381 tccattgaaa atcatgggaa gaggctacag ctatacgacg ttaaatgatc aagtagtaaa
    49441 aagtgtcacc gagattcatc aaggggattt gctgacgatc cattatcaag atggaatcgt
    49501 taaaggacaa gtacaagaga tagacaaagg agaataaaaa tatggaaaat ccaacttttg
    49561 aagagtcact gcaagaatta gaaaaaattg tcatgcagtt agaacaagga gacgttccat
    49621 tagaatcagc tctagattcg tttaaacgag gaatggaatt aagcaaacat tgccaagata
    49681 cattagctaa agcagaagag accttgacga aaatgatgac agaatcaaat gaagaagttg
    49741 attttgacgg aaacgaggaa agctgagatg agattaactg acttttcagc tattcatcta
    49801 cctgctgtag aaaaagaaat cttgtctttt ttagatgagc atacaacgga tagaggactc
    49861 tatgatgcga tgagttattc agtgaaagca ggcgggaaaa gaatacgtcc cttattactt
    49921 ttaacggccg ttgcttcctt tgatgagcct attgatgttc ccgtctatca agtagcagct
    49981 gctttagaaa tggtgcatac gtattccttg atccacgatg atttgcctgc gatggacaat
    50041 gatgatcttc gtagaggaaa acctaccaat cataaagtat tcggcgaagc attagctata
    50101 ttggccggag atggtttgct tacaggagca ttccagctaa tcagtatggc acacttaggt
    50161 aattctccta agcttctgct gcttcagcaa ttagctgttt gtgcgggatc acaaggtatg
    50221 gttgctgggc aagctgcaga tatcgagggc gaatcgaaaa agctttcttt agaagaatta
    50281 gcatttattc atgaaagaaa gacagggcat ttgattcgtt atgccttgct ggctggtggc
    50341 atattagcga agcaacctga agaaatctta ttgctacttc aacgattagc cgaacattta
    50401 ggtttagctt ttcagataag agatgatcta ctcgatgtga ttggtacaac gaaaacatta
    50461 gggaaaacag ctggtaaaga tgaacgtatg gaaaaaaata cttacccgcg tttattaggc
    50521 ctagaaaaga cacgtgaggc gttggagatt gagctgttat ccgccaacaa gatcatagac
    50581 aaattagaag aggacgtttt ttcttttgat ggcgggctga tgcgccagat gatcaagcaa
    50641 tttgatgtgg agaaatgagg gaaagaatgg aaaaagaacg cgtagatgta ttagcggtca
    50701 aacaaggatt atttgaaaca agagaacaag ccaaacgttc agtgatggct ggtttgattt
    50761 acaacgaaaa aaacgagcgg tttgataaac cgggagaaaa aattccagtg agtagtgaat
    50821 taagagtcaa aggtaaaaaa cttccttatg tttctcgagg gggattaaaa ttagaaaaag
    50881 cactcaaagc atttgatcta tctgttgaag gaaaaacatt gttagatatt ggtgcatcca
    50941 ctggcggatt tactgatgca gctttgcaaa atggcgcaaa aatgagttat gctttagacg
    51001 ttggctacaa tcagctagct tggaaaataa gacaagatcc tagagtcgtc gtaatggaaa
    51061 gagtgaattt tcgttatgca aaacccgaag actttacctt aggaattcca gaagttgctg
    51121 ttatcgatgt ttcttttatt tcactaaaac tgatgctgcc tccattacat gctattttaa
    51181 aagaagaggg agaagtgatt gctttaatca aaccccaatt cgaagcaggc agagaagctg
    51241 tagggaaaaa cggaattgtt cgtgaccctc agacccatca aaaagtttta gaagacattt
    51301 tgtcatttgc ggcacaaggt ggatatgatg tgctggaatt aagttattct ccgatcacgg
    51361 ggggagaagg aaatatcgag ttcttggctc acttgaggaa agtacccgaa agcggaacaa
    51421 tcaattcagc tattaatatg gcagaagtgg tttcaaatgc acacgaacag tttgaccata
    51481 aataaattgt aactgtgaaa atcaagtgag aagtatgcgg attcttcttt attataccaa
    51541 ggaaataagc tatcattgat taagaagaat atgctaagga gcgaagacgt aatgagaaaa
    51601 aaagatcggc atcgtttgat tactcgattg ttgagtgagc aagatatccg aaagcaggaa
    51661 gaatttgttg aaatcctgaa aagcaaagga atatctgtta ctcaagctac gatttccaga
    51721 gatataaagg aactgaaact gatcaaggta ccggcttcag atggcggcta ccgttatagc
    51781 ataccagctg aaacaaacga agatgtaaat actaaattgg aaaaactatt aaaagacgct
    51841 ttcgtggcag ttgaccaaat ggaaaaattt gttattttga aaacattgcc gggaaatgct
    51901 tcggcagcag caaatttgat tgataaacgt tacaagaaag aattattttc gattatcaat
    51961 gatgacgata atgttttgat gattacacga actgaagacc aggcagtttt attgaagaaa
    52021 gacttcctgc attaccttta agttgaggag cgaatgtgat gctacaagaa atcagtatta
    52081 ctaactttgc gattattccg gaattgcggc tttcttttca cgaaggaatg accgcgctga
    52141 ccggagagac tggtgcgggt aaatccatta ttatcgatgc tttaggtctg ttagcaggag
    52201 gaagaggttc aagcgattat atacgtcaag gagcagaaaa atgtgtgctt gaagggttgt
    52261 ttgaactacc aaaacaagaa ggattctctg aactgatggt tgaattaggg atcgaaacag
    52321 atgaggacaa tctgattgtt cgacgggata tgtccttaac tggaaaaaat gtttgtcgag
    52381 tgaatggtca tattatcaca ttagctaatc tgagaagaat cggcagttat ttagtagata
    52441 tccaaggaca aaacgaacat caggaactgc tccagcctga atcacatctt gctttattgg
    52501 atcgttttgg agatgtagct ttccaacaga agaaaaagag ttatcagcaa gaatatatct
    52561 cttatcgtga gctggaaaaa agggtacgaa aagtgcagca aaatgaaaaa tcctatgttc
    52621 agagaataga tatgctccat ttccagcaag aagaaatagc tgctgctgat ttgcaagtcg
    52681 gagaagaaga gaaattaaaa gaagaaagag aaaaacttag caattatcaa aaaattgctg
    52741 atggattagc tgcaggttac ggagcactga cgaataatga acaaaacagt gtagatggtg
    52801 ttggcttggc cgtttcagaa ctgcaaggga tcgcccatct agatgtggag tatgaagcaa
    52861 tttatgaaaa tatccaaagt gcttactatt tgctgcaaga tgcaatcggc gatatgagtc
    52921 gtcagattga tttattggag ttagatgaga gtcgtttaga agaagttacg caacgtttag
    52981 aattaatccg acagttgaaa cgtaaatacg gcgaatccat tgagtcaata ttggcttact
    53041 atgacgaaat caccgaagag ctagcttctt ctgatttttc agaaggtcaa ttggacaaaa
    53101 tgaaagagga actagagcaa aaagagcttc tacttcagca acaagccgca gatcttcatg
    53161 aagcgcgtaa aaaaatcgca aaagaattgg aaaaatccat tctgcatgaa ttgaaaagtc
    53221 tgtatatgga aaacactgag tttgaggtac gtttcttaaa agaagaaaat agacaattga
    53281 atcatgatgg atttgaccaa atcgaatttt acattacgac caatccagga gagccactga
    53341 agcctttggt gaaagtcgct tctggtggcg aactgtctcg gatgttgctt gctttgaaaa
    53401 ctattttttc ttctgagcaa ggtgtgacaa gtattatttt tgatgaagtg gatacaggtg
    53461 tgagcggaag agttgcacag gccatcgcag ataaaatttc tcaaatatcg aaatactctc
    53521 aagttttgtg catcactcat ctgcctcaag ttgctgcagt cgccgactat caatattata
    53581 ttgtgaaagc agtgattgat ggacgtacgc aaacttctgt ttcagagttg aaaacaaaag
    53641 aaagagaaga agaaattgca cgtatgctgg ctggaagtga gattacaaaa ctcacactcg
    53701 aacatgcaaa agaactattg aaactcgcaa aaaggacctg agccacctcg cagtggttca
    53761 ggtccttttc agcagctgat agctatattt tatttttaca tgacactcat tacagcagtc
    53821 aatacaacag atccaatcgt taaaatcagc attgtccaaa tgacaaattt tgttacttta
    53881 gcaaaagtac ttgtttgttt tttttcacgc attgttttca acctcgtcat taattagttg
    53941 attcaagtgt acaatctttt ttccatttcc acaagagggc ttttttataa aaacttccct
    54001 ttttttaaat aataggtagt tacgatacaa ataaataatg ttaatagaac tagccatata
    54061 aaggcatcat ccctttcagc aataggtaac ttaatgttca ttccatagat actgccgacg
    54121 attgtcggta tagttaaaat aatcgttaaa gaagttaaaa ttttcataat attattcaga
    54181 ttattcgata cgattgctga aaaagtctga ttcatttgtt ctactagttt taattgaatg
    54241 cgagaagtag tcattgcctg ctctgtttcc accaatacat cacgtaaatg atttttcaaa
    54301 gcatgatttt cttggaaaag tttattttgc gctagttctt tcaatgcatc taaattagca
    54361 gaagtcgctt ctctgaaata aacgaggctt ttttggatat ccatgatttg ataaagttgt
    54421 ttgttttcag tggatacttg aagtttggct tccaaagtat cgcattgaga ttgaagcgta
    54481 gctaaataac tgttgtaaga gagtgtgacc tgccaaatga attgaaagaa gatattgaga
    54541 ttaggcgaaa tatcactttt tggaaaggtc tggctaaaga ctttctttaa aaacagtggt
    54601 tcatgattcg taattgtgat aagcttcttt ttgggcgtca aaataataga gagcgggtat
    54661 gtatccactt gaaaataacc gctaggactt gttttagcaa aaggatattg taacaataat
    54721 aacgctggct ttgtcaactt tgattgattg aattcttctg tacgggaatt ttctgcatcg
    54781 tccaatatgg aagtgatgta atctgacgga atttcaaatt gatgtgtcag ctgatgaatc
    54841 tcttcttgat taggacgttc tacacaatac cagatcgttt ggtcagtttc ctttccggta
    54901 ttacgcagtt tctcattatc taatgtaaaa tatttgatca tgattttcct cctgttgcct
    54961 gtttctttta ttataacgga tagggaattg gaatacatgg aatgtgtttg taagatatag
    55021 aatataactg cataaattta aataaaatga aacagttgtg taaagtatat acagtatact
    55081 tgaagcaacg ataaaaaagg agtttacgtt catgggcaca ttcacaccac ttatcttgtt
    55141 ttggtatatt tacccggtaa tcgttttgtt tggttgccaa tttcttgtaa aattattttc
    55201 gttaaatcgt cgttttaaaa taaaagcccc tgatttggcg attccttttt tgtggggcgg
    55261 tatgcatgca ttgtctagaa atacagggac aatttctatc ttaccgtttt tctttatttc
    55321 tgttttgcta atgggaattt gtattgctgt tttccaagca tattattatg aagaaattat
    55381 ttatccgcgt tatttcaaga tgacttggcg tttggttttt ctgttgacaa tcgtcttgta
    55441 cgctgtcttg atcattgtga acatactttc ttacctatga aaaaaataga aaagctgtaa
    55501 aaagtctttt aaaaggcttt ttgcagcttg atttttaaaa aaatgtttat gaataaaaaa
    55561 agttgttccc ggatttccca gaaaaggtgg tagaaagtgg gggattgtgg tagactaatt
    55621 ttagttagtg gaggaatggg gggctttcaa tgttgatggg tgaattccaa cataatattg
    55681 atgcaaaagg acgtttaatc gtgccctcaa aacttcgtga agaacttggc gaaaagtttg
    55741 ttctgacacg cggattggat ggctgtcttt tcggctatcc gatgagcgaa tgggaaaatc
    55801 tggaagctaa actgaacgaa atgccgttgg ctaaaaaaga cgcacgtact tttgtgcgat
    55861 tcttctattc ggctgccact gaatgcgaac ttgataaaca aggacgaatc aatataccca
    55921 gcacgttaag aaattatgca gctttgacaa aaggttgcgt agtcatcggt gtatctaacc
    55981 gaattgaaat ttgggatgag acacgatggc aagatttttc tgcagcagca gaagagaact
    56041 ttgacgaaat tgctgaaagt atgattgatt tcggactata agaataggaa gaggctgact
    56101 aatgactgaa acatttcaac actatacagt catgttgaaa gaaacggtag atggattgaa
    56161 catcaaaccg gatggcgttt atgtagactg tacactcggc ggtgccggac atagtgaata
    56221 tttattaagc caactaagtt ctaaagggca tctgtatgcg tttgaccaag atcaaaaagc
    56281 aatcgatcac gccaagctgc gtttagcttc ttatgtcgaa caaggtatgg tcacatttat
    56341 caaagcgaac ttcagagaat tagaagaacg cttgagtgag catgtagata aggtagacgg
    56401 cattttgtat gatctagggg tttcctctcc tcagctagat gaagcagaac gtggatttag
    56461 ttatcatcaa gatgcaccgt tggatatgcg catggatcag tcagctcctt tatccgctta
    56521 tcacgtaatt aatgaatatt cctaccatga attagtaaag attttcttcc gctatggcga
    56581 ggagaaattt tccaaacaaa ttgctcgtga aatcgaacga gtacgaaaaa caaaaccaat
    56641 ccaaacaact ggtgagcttg tcgaaatcat taaacaagtc atcccagctc ccgcaagaag
    56701 aaaaggcggg catcctgcaa aaagaatttt tcaagctgtg cggattgcag taaatgacga
    56761 actcggtgcc gaagaggcct ctttagaaca ggcaatccgg ttattgaaag tcaatggaag
    56821 aatcagcgta atcacattcc attcattgga agacagaatt gtcaaaagta tgtttaaaga
    56881 atatagtacg gtacaagatc taccaccggg acttcctgtc atacctgacg aattccaacc
    56941 agaactgaaa gtcattactc gtaaaccaat ccttccttca gaggaagagc taacagaaaa
    57001 caatcgatca cgcagtgcaa agttacgtat tgcagaaaaa ataagaatga aagaggagta
    57061 gaatgatggc agaattaaag aaaatgcaag attacgaata tgatttacct gaattggtgg
    57121 atcagccgga acaacaaatg cctgattctt cttcgaatca agaaatcatt cttccacaat
    57181 caccggcaaa acgattaaag cagatttctg ctttggaaaa aattgctgtg gcaagcgtgg
    57241 cgattgctat tgttgcttta tgtgttttaa cagttatgct gcgcacaaat atcagcggag
    57301 ctgaaaaaga aatcacgtca atacaaacag aaatcaatga taaaaagcaa gaaaaaacga
    57361 gtttagaaca agaaaaaaac gaactttctc gtactgaacg gattaaacaa atcgcagaag
    57421 aaaaaggatt gtctattaat gacgacaatt taaggaaggt gaagtaaatg agtcttaaaa
    57481 ataaaattcg gcggtttatg gaaaagaaaa atctaaatcc gatgaacaat cgcaaaaaag
    57541 taggtatcat tttatttgct acgagtattg gattgttctt tttatttgcc gtccgtttct
    57601 cgtatatcgt tattggaggt catgttgctg gtacctcatt agctgaaaaa acaaaacagt
    57661 tgtaccaagg aagtgaagta gtaaaagcaa aaagaggaac gatttatgat cgtaacggtg
    57721 tggcgctcgc ggaagatgcc tcctcttatt caattaaagc gatactctca aaaacatata
    57781 cctctgggga taaaaaactg tatgttgaag aaaagaattt tgataaaata gcagaaatcc
    57841 tgcataagaa tctttctata gacaaaaaag atgcactgaa tattttagag gacggcgcaa
    57901 aaaaagagct gtatcaagtt gaatttggta gctatgggaa aaatatctcc caagaaacca
    57961 agcaaaacat cgaagcggac atgaaaaaag aaggtgtcgc tggtctttat tttgtcgacc
    58021 atcaggctcg gatgtatcca aatggcgtct tttcttctca ttttattgga tacgcagttc
    58081 cagacaaaga tgagaatggc ttagttggta aactaggttt ggagtctgct tacaatgata
    58141 tcttgagcgg taaagatggt aagattattt atcaaaaaga taattttcaa aatcctctgc
    58201 caggtacagt ggcagaagaa gaaaaagctg tcgacggaca agatatctac acgactttag
    58261 acagcagact acaaagttat ttagaaacac tgatggatca agtaaatgaa gaatatcagc
    58321 cagaagaatt gacagcagtc ctgatgaaag caaaaaccgg agaaatcttg gcaatgggac
    58381 aacgacctac tttcaatcct gaaacaatgg aaggattgac tggaaaagat gctatctggc
    58441 gtaatttctt ggtacaagat agttatgaac caggatcaac catgaaagtt ttcactactg
    58501 cagctgctat cgaagaagga gaattcaacg agaatgaaac cttccaatct ggaaaaatcc
    58561 aagtagaaga tgctacgatc aatgaccatg actttggtga aaaaggtgtg ctgaccatgc
    58621 gtcaagctct ttcatggtca agtaacgtcg ggatggtcat actggaacag cgattaggcg
    58681 gacgatggta taactattta caaaaactgg gatttggaca aagtactcat tctggattag
    58741 atgatgaagt aaacggtgct ttacctacat taaatattgt cgaccgagca atgagtgctt
    58801 atggacaagc ggtaggtgtg acgaatttcc aaatgatgaa agctttcact tctattgcga
    58861 ataacggtac aatgattcaa cctcgatata tcagcaaagt ggtggatccc caaactggag
    58921 aagagcgaac aactcaaaca gaagttctag ggcaaccttt ttcaaaagaa acaacagaaa
    58981 aagtccgtga atatatgaga gatgttgtcg aaagcgaaaa ctacggtagt gcttatggtg
    59041 tatatagtgt acctggatac aatgtatcgg caaaaaccgg aacagcccaa atcgcttctg
    59101 atacaggggg atatcaaaca ggagatacag cctacctgta ttcgattgtg gagatggttc
    59161 cttctgaaga tccagactat gtcttgtatc taacaatgaa acaccctaag acatatgatc
    59221 gaatggcatt ggctaaaatc gcaaatccat tgatgaaacg ggcaatggat tttaaagaaa
    59281 ctgaagaaga ttcagataca gaaacaaaaa cagaaaaagt atccgtagcc gattatcgta
    59341 atcttgaagc ggacgttgca gcagctgatg ctcaaaaaag tggactgcaa cctgtagtga
    59401 tcggtaatgg taaaaaagta caaaaacaat ctactgccaa cggtgatcag ttgatctctg
    59461 gagaaaaact gatcctatat actggtggtg ataagctgat gcctgacgta acaggatggt
    59521 caaaagcaga tatcatgaag ctggggaaaa tcctcggtat tgaagttagt tttgatggag
    59581 atggttattg tgtcaaacaa gaacttgctc catatgaaaa aatcactgaa gacaaactta
    59641 gttttacttt agaagaataa gaagagaaat aggagagaaa agagcatgga atggacacaa
    59701 gcattgattc ccattgtcag cagttgtgca atgacaattg ccgcaatgcc gctatttatt
    59761 ggttattttc aaatgaaaaa gcaaggtcag gcgattcgtg aggagggacc aaagtggcac
    59821 aatagcaaag ccggaactcc tacaatgggc ggcttagtgt ttctgatcgg ctctattttg
    59881 acaggaattt gggttggagc ttggcaaaaa cagctaactc ctactctttt catcttgctt
    59941 tttgtattag cgttatacgg agtaatcggc tttcttgatg attttataaa aatattcaag
    60001 aaaagaaaca tgggactgaa ctcgaagcag aaattgattg gacagattct agggggaatc
    60061 atcttttatc ttgtttaccg ttctgaaggt tatccaggtg tattgaattt cttcggacta
    60121 gatctacctt taggaatcat ttatggtatt ttcgctctct tctggcttgt tggattttca
    60181 aatgcagtaa atctgacaga tggcatcgat ggattagttg ctggcttagg aagtatctct
    60241 tttgcagctt acggtctaat tgcttggcat cagcatcagt atgatgtatt aatcatttgc
    60301 ttaagtgttt taggaggttt actgggtttc ttcgtctaca acagaaaacc tgccaagatt
    60361 tttatgggag atgtcggttc attagcgttg ggcggacttt tagcggcaat ttctataatg
    60421 ctaaaccaag aatggacatt gcttttgatt ggtttgatct atgttatgga aacagcaagt
    60481 gtgatgcttc aagttacttc ctttaagctt acaggaaaac gaatttttaa aatgtctcca
    60541 attcatcatc actttgaaat gtgcggatgg tcggagtgga agattgatac agttttttgg
    60601 ttgatcagca ttgtgacttc gttgatcaca ttatggatcg tttggtagga gatgacggga
    60661 aatgaaaaaa atatcaacat atgaaaataa gaaagtgctt gtcttaggac tcgcaaaaag
    60721 cggagtaagt gcggccaaat tattacatga attgggcgca cttgtcaccg ttaatgatgg
    60781 aaaacctttt gacgaaaatc cggaagcaca agagctgctt tctttaggga taaaagtaat
    60841 aacaggtagt catccaatcg aattgttgga tgaagaattt tcattgatgg tcaaaaatcc
    60901 agggattccc tacagtcatc ctttagtagc aaaagcccaa gaaatgggta ttccggtaat
    60961 tacggaagtt gagctagctt acgaagtagc ggaatgccca attattggga ttacaggaac
    61021 gaacggaaaa acaacgacaa caacgatgac aggactacta ctgaatgcag gagcagatca
    61081 aggaattgca cgtttagcgg gtaatattgg gtatccagca agtggtgtag cgcaagaagc
    61141 aaaatcagaa gataaaattg tgatggaatt gtctagcttc caactcatgg gaatcacaga
    61201 tttccgccct catatagcgg tgattaccaa tatctatgaa gcacatattg attatcatgg
    61261 tacaagaaaa gaatatgtca aagctaaatg gaaccttcaa aaaaacatga cagaaaaaga
    61321 ttatctgatc cttaactgga atcagtcaga actacaagaa ttagcgcaaa gaacaaaagc
    61381 acgcgtactg cctttctcga caaaagaagt gttggaagac ggtgtgtatg ctgacgatta
    61441 ctcaatatac tataaaaaag aaaaaataat ggaaatatct gaacttggcg taccaggtaa
    61501 gcataatgta gaaaatgcac tagctgctat ttctgtagca aaattgtatg gtatatcaaa
    61561 tgaagctata cgagaaacgt tgcatttttt ccatggcgta ccacatcgta cgcaatatgt
    61621 aggagaaatc caaggcagaa aattttataa tgattcaaaa gcaaccaata ttttagcgac
    61681 taaaatggct ctaagtggat ttgaaacgtc taaggtcgtg ttattggctg gcggattaga
    61741 cagaggaaat acatttgatg aattaattcc ttcattaaaa ggaatcaaag caatggtcgt
    61801 atttggccaa acaaaagaaa agttgatgga tgctggaaaa aaagcaggca tcgagacaat
    61861 agtaacagcc gattctgtag aacaagcagt tcctttagca ttagaaaaca gtacagatgg
    61921 tgatgtagtt ttattatcac cagcgaatgc cagctgggat caatacccaa attttgaaac
    61981 tagaggaaac agattcatgg aagctgtgaa ccgattaaaa tagaaaaagt agaagcgagc
    62041 gataaaaaat gaaaattttg gttacaggcg gaggaacagg gggacatatt tatccagctc
    62101 ttgcctttgt caattatgta aagactcaag aaccgaatgc tgaattcatg tatgtgggag
    62161 caaaacgagg tctagaaaac aaaatcgtcc ctgatacagg aatgcctttc cataccttgg
    62221 agattcaagg atttaaacga aaaatttcca tgcataacat caagacggtc cgattgtttc
    62281 ttaagagtat ccgtcaagca aaaaaaatcc tcagtgattt tcaaccagat atcgttatcg
    62341 gtactggagg atatgtttcc ggagcggtag tgtacgctgc agccaaaatg ggtattccaa
    62401 cagttatcca tgaacaaaac agtgtaccag gtgttaccaa taaattttta agccgatatg
    62461 tggatcggat cgctctttca tttaaagatg cggcttcctt ttttcctgaa aacaaagcag
    62521 ttcttgtagg taatcctcga gcacaagaag tagcagatac taaaaaatca gatattttga
    62581 aaacatttga tttggatcca gaaaagaaaa cagtcttgat ttttggcggt agtcaaggag
    62641 cacttaaaat caatcaagca gtatcagttt ttttaagtga atatattcct tcttcttacc
    62701 aagttttgta tgcttcagga gatagatatt atgaagagat cctaaataaa ataaatcctg
    62761 aaatacaaaa tattagtgtc cagccttata tcaaaaatat ggcagaggtc atggcttcca
    62821 gtgatctgct ggtggggcgt gctggtgcaa cttcgatcgc agaattcaca gctctggggt
    62881 taccggcagt gttggttccc agcccatatg ttacgaatga tcatcagact aaaaatgcaa
    62941 tgagtcttgt tcacgcagga gcagcaaaaa tgattgcaga taatgaacta acaggtgaat
    63001 cactaagtca gacagtcaac gaaatcatgg gtgatgaaga attgcaaaag caaatgtgcc
    63061 gagcttctaa agaacaagga atccctgatg cttcaaaacg tctttatgac ttagtaaaac
    63121 aaataatcaa aaaataaagg cggtggtctt agtgagtaag aaaaaagaac caaaaaaatt
    63181 gtctcatccg tcttctgatg aaaacaattt gactccttgg caaaaggcta accgaaaata
    63241 tctggcagaa caagagatga aaaaccaaga gccaaaaaaa gaagacataa cattagatac
    63301 acccctttca gattctgaag aagaaaaaac ggttcaagag gaacagcaag aagaaagcca
    63361 gtccgagaaa aaaatcgaat tttttgaaga gatcaagcaa aagaaaaacg gtggaccata
    63421 taacggttct tttttgaacc ggctgcccaa tttaaaaagt caacggcaca aagtactatt
    63481 tcgtcggtta gctttgatca tcactgtatt gggtataccg ctgatttttt tgatctatta
    63541 tgtttctcct tatagcaaat tacaagcagt tgcagtatca ggcaataaga cagtcaacag
    63601 ccaagaaatc atctctgata caaaattgtc attaggagaa aatgtttggg gacagtattt
    63661 tcatcgatct acttatatcg atcgccttaa aaaagcacag ccaagaatcg aaacagcaaa
    63721 tatacatttc aaagggatga atgaattcga tttggatgta acagaatata aagagattgc
    63781 tttgatcgca aaaaacaatc aatactaccc agtgattgaa aatggaacag tattagatga
    63841 aaaagtagca aatcctacaa aaaatctccc tatcttggaa gagttcaaag actctgcaaa
    63901 aatcaaagaa ttaacaaagc aatacaatca gttatcatca gaattacaaa aagcaatttc
    63961 tgaaatcaag tacacgccaa gagcaagcaa taaaaatctg atccaattga atatgaatga
    64021 cggcaatcaa gttattgtaa atatcaataa tcttgcgaac caaatgaaat attattcgca
    64081 agtagccaaa gatatggatg aaaaaggtgt gatcgacatg gaagttggta tcttctcgca
    64141 tccttacggt agcactcaag aaacggaaga atcgggccaa tcggaagaaa caactgaaag
    64201 tgaagaaaat gcggatcaaa caaacgaatc aagtgaagag gcagaaacgc aaaaagcaca
    64261 aacacctgga tcgtctagca caaacggatc atctgattca aatgattcaa gtgaaacaag
    64321 tcaaatagaa gataatccaa gtacaactcc gtcaagttta aatgaaaatt gataaaacaa
    64381 ctttaaaaag taagaatgta ctttctaaaa accagtaatt tatggtagaa ttggaagcag
    64441 tgaatatatc tattaataag tatattttaa aacggacgct agttgttcgc ttgtatatgg
    64501 aatagtaagt aggagggaat cccattcatg gcaaaaacag gaatgtatgt aggccttgat
    64561 attggaacga catctgttaa agttgtcgtg gctgaatata tcgacagcca aatgaatatt
    64621 attggagtag gaaacgcaaa atcagaaggg attaaccgag gcattatcgt tgatatcgat
    64681 aaaacggtcc aagcgataca acgagcagta cgacaagcag aagaaaaagc aggtatccag
    64741 atcaaaggcg taagtgtcgg tttgccagca aacatgcttg aagtagaaaa ttgccaggga
    64801 atgattgcag ttaacggaga ttcaaaggaa atcaccgatg aagatgtcag aaatgtggct
    64861 tcagctgccc ttgttcgttc aatacctcct gaacgtcaaa tcgtgtcaat attgccacaa
    64921 gattttactg tcgatgggtt cgagggaatc aaagatcctc gtggaatgat cggcgtaaga
    64981 ttagaaatgt acggtctgtt gtttacagga cctaaaacga tcatccataa catacgtaaa
    65041 tgcgtggaaa atgccggatt aatcgtcaac gaaatggtga ttactccttt agcgctgact
    65101 gaatcaattc tttctgacgg agaaaaagat tttggtacga tcgtcattga tatgggtggc
    65161 ggccaaacaa caacggctgt tatgcatgat aagcaattga agtttaccaa tcttgaccaa
    65221 gaaggtggcg aatttgtcac taaagatatc tctattgtat taaatacgtc atttaacaat
    65281 gcagaagcgt tgaaaataaa ctacggggat gcatacccag aacggacatc accagatgaa
    65341 gaatttccag tagatgtgat tggtcagtca gaaccagtga aagtcgatga acgctacttg
    65401 tcagaaatta tttcagctcg aatggaacag attttcaaca aagcaaaaga tgcattggac
    65461 caaatcgaag cattagagtt gccaggtggc gtcgtattga caggcggagc tgctagtctt
    65521 cctggtgttg tggacttggc acaagaaatc ttcggcgtaa atgtaaaatt gtatgttcca
    65581 aatcacatgg ggctgagaaa cccggtattt acaaatgtaa taagtatcgt ggattattca
    65641 gcaaacttga gtgaagtgta tcaattggca aaaattgctg ttacgggtga aacctcagca
    65701 gctcgtcaaa tggtagttga acaagaaaca acaaacacat atgagagcta tgaagcacct
    65761 gaagaaacaa tctacgatga gccggaacca aaagagtccg gagaaaatgt aaaaaataaa
    65821 atcaagggtt tctttacaaa tatttttgat taattcatcc tgggaggaat aagatatgga
    65881 attctcaatt gataataata tcaatgacgg cgcagtaatc aaagtcatcg gagtcggtgg
    65941 tggcggtgga aatgctgtaa accgaatgat tgaagaaaac gttaaaggtg ttgaattcat
    66001 tacagccaac acagacgtgc aagcattaaa aaattcaaaa gctgaaacag ttatccaatt
    66061 aggccccaaa tatactcgtg gtttaggtgc tggttctcag ccagaagttg gacaaaaagc
    66121 ggcagaagaa agtgaacaat ccttacgcga agctttagac ggtgcagata tgattttcat
    66181 cactgcagga atgggtggag gaaccggtac aggtgctgca ccaatcgttg caggtatcgc
    66241 gaaagaattg ggcgcattga ctgttggtgt cgtgacacgt ccattcactt ttgaaggacc
    66301 gaaacgtggc cgttttgctg cagaaggtat cgctcgtttg aaagaaaatg tggatacatt
    66361 attaattatc tcaaataacc gtttattaga agttgtagat aaaaagacac cgatgcttga
    66421 agctttccgt gaagcagata atgttttacg acaaggtgta caaggtatct ctgatttgat
    66481 cacagcgcca ggttacgtta accttgactt tgctgacgtg aaaacagtga tggaaaacca
    66541 aggtacagca ttgatgggga tcggtgtggc tagtggagaa gatcgcgtag tagaagcaac
    66601 gaaaaaagca atctcctctc cattgcttga aacatctatt gatggagctg aacaagtact
    66661 tcttaacatc acaggcggtt tggatatgac attgttcgaa gcacaagatg cttcagatat
    66721 cgtagcaaat gcagcaactg gcgatgtaaa tatcattcta ggtacatcta tcaatgaaga
    66781 aatgggcgat gaaatccgtg tgacggtcat cgcaacaggt atcgatgaat caaaaaagga
    66841 acgcaagtct agccgaccag ctagacaagc acaaatgcaa tcacctgctc aaaagactgt
    66901 attagatatg gatcaagcta aaccaacttc ttcagaagaa gaaaacagtt ttggcgattg
    66961 ggatatcaga cgtgaacaaa atgttcgtcc tagagttgat gattccaatt ttgacaatat
    67021 cgaaaaaaaa gaatttgata cattcaaccg cgaagaaaca aaatctcatg gcgatgatga
    67081 attaaatacg ccaccgtttt tccgtcgtaa aaaataaagg agaggaagag cacatgattg
    67141 ctgacaactt gcagaaaatc aagcaagaaa ttcaggattc gtgtgctctt gttcatcgtg
    67201 atccgaaaga agttacatta gtagctgtaa caaaatctgt tgaatccgct caagctgaag
    67261 aattggttag agctggcgtt atgaattgtg cagaaaaccg agtagataaa ttattagaaa
    67321 agaaacaaga tttatttgat tttcctgaaa taaagtggca tttaattggt aatttacagc
    67381 gcagaaaggt aaaattgatt gtaaatgaaa ttgattactt tcatgcgttg gacagtttaa
    67441 gattagcaga agaaatccag aagcggttag agaaagaatt atcctgtttt attgaagtaa
    67501 atgtttctgg tgaggaatca aaacatggaa tttcaccaga tgagctttta ccgtttgtgg
    67561 aatctttagc tgcctgcaca aaaatcaagg ttgttgggtt gatgactatg gcgcctaaag
    67621 aagcttctga tgagcaaatc cataaagtgt ttggtacgct gaaagaattg cgtgatcttg
    67681 ttcaagcaaa aggttatgcc catgcaccat gtagtgaatt aagtatgggg atgagtcaag
    67741 actttcaaat tgcgattgaa gaaggtgcaa catttatccg tgtaggaaca gcgctattca
    67801 aagacgaata aaggaggaac aaaaatgccg cttttaaaca gaggagcaat tgctaatttt
    67861 ttcggattag ctgatgagga agagtacgaa taccaagatt atcctgaaac aacaaaaaag
    67921 gctgtacagc caactaaaaa aaatacaaca cagaatcaat caactcattc tgtatctgct
    67981 gcgaatactc atgaaaaaca aaaaacggcg acttcacgtc ctgtaacgca tgaaagtatt
    68041 cagccggctg aaaagaaaaa agtaattgaa ctacatcaat cacctacaaa aactgtagcg
    68101 aaagaaagaa cagcttcaag aacctctaca caacctaaag caaatcttgc tggaaaaatc
    68161 actgtaatgg aaccaagagc ttattctgaa gctatggcga ttgctaaacg aatcattgct
    68221 ggagaagcaa ccttagtgaa ttttcattta gtagaagaaa aacaagcgag aagaatcgtt
    68281 gattttctaa caggaacagt ttatgcacta gatggagata tcaaacgtgt tggagatgag
    68341 atttttcttt gtacaccgac tgggatggag attgacagtt caaccgctca atcttttgca
    68401 gatgcgaatt tgtttgatct gtaagaggag gaactacgat ttatactatt gcactattac
    68461 ttcatttact tcaacgggca gcctatttct atagcattat cttgatggta tatgcattgc
    68521 tctcttggtt tccaggcggg tatcaatcag ggctaggacg attattagct aagatctgcg
    68581 aaccgtatat cagtttgttc gatcgtctgc ctttgaggat tggacccttg gatttcacaa
    68641 ttttaggggc aatcctgatc ttgaacttag ccgtacaagg tttagcacag atcgttatat
    68701 ggataactgt ccatttagtt tattgattga agcaacaaaa tctttattct cagaatagat
    68761 aggaggggaa aattttggat gcaaatgtat accagcactt tcgggcagat gaacgtacat
    68821 ttatcgattc ggtaggcgat tggattgaac aggttcaagg acaatatgca ccctatctga
    68881 ccgaatttct tgacccaaga caatcctata ttttagaaac actgattcga caaagcagtg
    68941 atttgcgttt catgttttat ggcggatatg aacaggcaga aagaaaaaga tgtttgattt
    69001 tccccgatta ttacgaacct gtagaggaag attttgaaac agcattgtat gaaatccatt
    69061 atcctagcaa atttgccagc ctaagtcatg ggaaaatcct tggaacttta gtgggaacgg
    69121 ggatcaaacg ctcttacttt ggcgatatca tctcggacgg tgatcgctgg caagtattta
    69181 ttgcaaatga ggtggatcat tttgttgctg cacaagtaac aaagattgga aaggttgctg
    69241 tacgcttgga accaatcaaa tacacagaaa tgattatgcc gaaagatagc tggacacaag
    69301 aaagaggaac agttagttcc atgcgactag acagtgtgat ctctgagatt tataatattt
    69361 ccagacagcg gtccaagcag ctgatcgaag ctggtaaagt aaaagtgaac tggacagaaa
    69421 atacaagacc tgattttgta cttgaactat tagatattat ttcagtcaga ggttatggca
    69481 gagtccagat ccaagaactt gaggggaaga cgaaaaaaga aaagtacagg gtactgttcg
    69541 gtgtattacg taaataaaat aaaaaggtgg tttactcatg gctttaactc cattagatat
    69601 ccaaaataag acattcccga caaaaatgcg gggatataat caagacgaag tagatgattt
    69661 cttagattta gttgttcgtg actatgagga attgactcaa agaaatcgag aattagaaaa
    69721 agcagtcaaa cattctgaag aaaaacttga gtacttcaat gaattaaaag atgctttgaa
    69781 ccaatcaatc atcgtagctc aagatacggc tgataaagta aaaacaagtg caagcaagga
    69841 atccgaagtg atcgttactt ctgcacaaaa caaagcagat gagttagttg cgaacgctga
    69901 aaaacgtgca catcaattaa caactgatgc agaagaaaaa gcaagaaaaa tcttgacaga
    69961 tgcaacagaa aaagcaagac agcttgcgac agaaactgaa gatcttaaga aaaaaacacg
    70021 agtattccat caacgtatca gcttgatgct tgagtctcaa ttagaacaag tgaaaagtcc
    70081 ggaatgggat gaaattttac aaccattctc cagctacgtg actgacagcc atgaagtgat
    70141 caaagaagtt ttagcaaaac agcttgacaa tgaaaatgat actgatgtaa actcagacac
    70201 aggagtcata gaagcaccta tgacggcatt tgatacacag gctattgata tctcgatcgt
    70261 gcctggtgaa aatgaaaacg agtagaacag aaaagcactt tgttattctt aaagcgagcc
    70321 gatgagagtg gaagttcggt aagacactgc atggtgaaag atcctctact tgagacctgc
    70381 ttttccgaaa ttagagtaag aagcagcggc tgacctcgtt attggtcaca gagagtttgc
    70441 actagataga tgcaactaaa tttgggtggt accacggcat gttcgtccct tgcggctgaa
    70501 catgcttttt tgtgtccgga aaacaaataa aggagtgtat ggttgaatga aaatgaaaga
    70561 aaccttgcaa ttaggaaaaa caaaattccc tatgcgtggt aatttgccaa accgagaagg
    70621 cgagtggcaa aaagaatggg aagaaaatcg aatctaccaa aaaagacaag agttgaacga
    70681 aggaaaacct agctttgttc ttcatgatgg acctccatat gccaatggta atatccattt
    70741 aggacattca ttgaataaaa tcagtaaaga cattatcatt cgttcaaaat caatgtctgg
    70801 ctatcgtgca ccatatgttc caggatggga tacacatgga ttgccgattg aacaagtatt
    70861 ggctaataaa ggcgtgaaac gtaaagaaat gtccatggca gaatacttgg aaaaatgtcg
    70921 tgaatatgca ctttcccaag tagataagca acgagcagat ttcaaacgcc taggagttgc
    70981 aggagattgg gaacatcctt atgttacatt agatccttct tatgaagctg cacaagtacg
    71041 cgtatttggt aaaatggctg aaaaaggcta tatttacaaa ggattgaaac caatttactg
    71101 gtctccgtca agtgagtctt ctttagcgga agctgaaatt gaatataaag atgtaaaatc
    71161 tccatctatt tttgtagctt tcaatgtcaa agacggcaaa gatattcttg atgaagatac
    71221 agcttttgtc atttggacga caacaccatg gacattgcca gcaaaccaag gtattgctgt
    71281 aaatccaaaa tttacgtatg tattggtgga agccgatggc cgtaagtttg tcgtagcgaa
    71341 agatttgcta gaaacagtcc aatcagaaat cggttgggaa aatgtaacag ttctaaaaga
    71401 atttgctgga caagaaatgg aatatatgac tgcaagtcat ccattctatg aacgtgaatc
    71461 acttgtgatt ttaggcgatc atgttacttt agacgctggt actggattag tccatactgc
    71521 tccaggacat ggggaagatg actatatcgc tggtagcaaa tataaactat ctgtagtgtc
    71581 acctgtagac tcaaaaggat tgtttacaga agaagcacct ggatttgaag gtatctttta
    71641 tgacaaagca aatcctatga tcactgatct attaaaagaa aaaggagctt tgctgaagct
    71701 agatttcttt actcatagtt atcctcacga ttggcggacg aaaaagcctg tgatctatcg
    71761 ggcaactcca caatggtttg catctatcga taaattccgt cagaatattt tggatgaagt
    71821 agaaaaagta gagtgggtta tcccatgggg taaaacacgt ttgtataata tgatccgtga
    71881 ccgcggcgat tgggtaattt cccgtcagcg tgcatgggga gtaccacttc cgattttcta
    71941 tgctgaaaat ggagaagcaa tcatcacacc agaaacaatc gaacatgtgg caaaattatt
    72001 tgctgaacat ggatcaatta tctggtttga aaaagaagcg aaagaacttc ttcctgaagg
    72061 atttacccac ccaggttctc ctaatggcga atttacaaaa gaaaaagata tcatggatgt
    72121 ctggtttgat tctggttctt ctcatgaagc tgttttacgt cagcgaccag aattatcatt
    72181 cccagcagat atgtatttag aaggatcaga ccaatatcgt ggctggttca actcaagtat
    72241 cacgactagt gtggctatca atggtgtagc accatacaaa tcagttattt ctcaaggatt
    72301 tgctttagat ggtgaaggcc gtaagatgag taaatcttta ggaaacatca ttgcaccaga
    72361 aaaagtgatc aaacaatttg gtgcagatat ccttcgctta tgggtatcta gtgtagatta
    72421 tgaagcagat gttcgtgttt ctatggatat cttaagtcaa gtggcagaag tatatcgtaa
    72481 aatcagaaat acgatgcgtt tcttattagc gaatacagaa gatttcgatc ctaaaaaaga
    72541 tactgtttcg tacaatgatt tacgttctgt agacaaatat atgaccgttc gattgaacca
    72601 agtaatcaaa gaaatccgag aagaagggta cgacaaatat aacttcatgc atatttaccg
    72661 tacagttatg aacttcttga cggttgatct atcgtcattc tacctggatt ttgctaaaga
    72721 tgtagtttat attgaagcag aagatgatta tcaacgtcgt tgcatgcaaa ctgttttcta
    72781 tcaaacagct gtagctttga caaaattatt gactcctatc attccgcata cagcagaaga
    72841 aatctggtca ttcctgaaag aagaagagga gtacgttcag ttaagcgagt ttcctggtta
    72901 tgaagagttt gcaaaccaag aagaattgat ggatacctgg actgctttca tggacttccg
    72961 tgataatgtc ttaaaagcat tggaagaagc aagaaacagc aaattgattg gtaaatcttt
    73021 ggaagcaaaa ctaactgttt atccaaaaca acaagttcgt gaaatgttaa aagctttgga
    73081 tgcagatatt gcacaattat tgatcgtttc accagactac tttgaagtta tggcagcaga
    73141 tgaacaagtg ccagataacg ctatggtatt tgatgatgtt gctattacag tggaaaaagc
    73201 agacggggaa acttgcgatc gctgtcgcca agttcgtaaa gatgtaggag tagatgaaaa
    73261 acttcctcat ctatgcggtc gttgtgcaaa aatcgttgaa gacttctatc ctgaagctgt
    73321 agctgaagga ttcgaagaaa agtaaaaggg aaaagggggt gtgacatgtt ctttgtcaca
    73381 cccccttttg ttatcaatcg tttaattttc cacgttcacg ataccagtta tctggttgcc
    73441 agatccattc gaatccgtct ttttcaagta gttcgaaagc tgctttaggt cccattgatc
    73501 cagctttgta gttgggaaaa tccggttgtg tttggtccca agcttttcga atgacatcca
    73561 cgatatgcca agattgagcg acttcttccc aatgagagaa gttcgttcca tcaccattca
    73621 acgcatctaa tagtaatttt tcgtaagctt ctgggctatt ttcaaccatt tctgcactat
    73681 ttctaaattc caatttgatt ggttccgttt caaatccttg ccctacttct ttcccattca
    73741 aggataaaga aaatccttca gttggctgaa tatagatcgt tagaacatta ggtggaagtg
    73801 tcgtatcgtc acatggttca tcgacagatg ttttgaatac attaacagga acttgtttga
    73861 atacgatgtt gatccgagtc cctttttcag tcaaacgttt gcctgtacgt acatagaaag
    73921 gaacacctga ccaacggaag ttatcgatag tgaatttgcc tgcgacgaat gtttcagtct
    73981 ctgattgagc atccactttg tcttcttcgc gatagccctt gaattgtaca ccatccaatt
    74041 caccttgagc atattgcccg cgaacgaagt tttctaacac ttcttcttcg ttataaatgc
    74101 ggatagcttt caatgctttg atcttttctg tacggatttc tttttcggaa aatgctactg
    74161 gcggttccat tgcaagtaat gctacaacct gtaaaatatg attttgcacc atgtctttaa
    74221 gtgcgccact atgatcgtag tagcccccgc gatcttctac accgaggttt tcgccaaaag
    74281 taatttggat attgtcgata tagcgattgt tccattgagc ctcaaaaata ttattggcaa
    74341 aacggatagc tgagatgttt tggatcattt cttttcctaa ataatgatca atcctgaaaa
    74401 tatcttgttc tgggaatact tctctgattt gctggttcaa ttcaaaagca gactggtaat
    74461 cagaaccgaa cggtttttct atgatcaaac gattgaagcc attttttgtc ataatatttt
    74521 gtgatttcaa atgagaaacg atcgttccaa aaaattgagg tgccatagcc aaataataga
    74581 cacggttacc gtttaattga tagtgtccat ctaatcggtc agacaattct tttaatgtct
    74641 gataatgcgt tgaatcattt acatcatggg attgatagta aaaatggctt gcaaaggtag
    74701 ttgcttcttc atctgaagga ttcaatgcac ggattgtttc tttgacaact tcacggtagt
    74761 gctcatcgct ccagggacgg cgagcggttc cgattacagc aaaatgctcg cctaaatttc
    74821 cttttctgta aaggcggaac aaagacgggt acaatttacg ctgggctagg tcacctgtac
    74881 cgccgaatat cgtaaacaat acattttttt cattagacat gtggatacac tcctttatat
    74941 tttatcgaca aaaatcgtgc tggccgcctt aaagctgacg gggattttct ttctatcatt
    75001 ttggatagtg atcggtcctt cataagctgc gatgtcaacg atctcatatt cttcgttgat
    75061 attcaggtca atagtgacta agtaatccaa caattctttt tcatcaagca ctcgtgcgat
    75121 acgcactttt gtaccgacag gataggactc caatgtttgt cggcgttctt catggacaac
    75181 ttcctctttt ttaggaatcg atcctccatg aggacaatgt tcaggatgat tcaaatagtt
    75241 ttctaaatga tctgcaagat gttccgaagt cacatgttcc aatacttcag catcatcatg
    75301 gacttcattc cagctatagt ctaaatgttc aactaaaaaa acttcccata agcgatgttt
    75361 gcgaatcaga ctgctagctt tttgcagtcc gcttgcagtc aattgtacac cttgataagg
    75421 ggaatgttct acaagctctt ctttcacaag tttagaaatc atttcactga cagatgcagc
    75481 tgagacatct agactagata cgatttgttt attgttgact ttcgttgtat ctccacctag
    75541 ttctaaaatg attttcaaat aatcttctcg atttggtgtc atattcgatc atcctttcat
    75601 ggtaaaaaac catcattagt aagtgtaaca aaaaaaacgt taattgacta tttttctaaa
    75661 taaaaaacaa aggattttta gacgttttta caacaaaaaa cgaaagatga tagagaagac
    75721 tcattctttc gtttataaag gatatatata tacattggga gggatctatt tttcagataa
    75781 caacttatat gaagtgtctg cagattcgtc ataaagagtt tgttcgggat aagaaaagat
    75841 aagataaaaa ccatactgat cacgtacgac taaacgatat tcatctattt ctaccacaga
    75901 tgtgccaaga gactgaccgt tgatcaaaaa cgtgtaatca gatgtaatct gtaattgttg
    75961 tttttgctgg ctatcactaa agaaccatgt accttgatac tttgagagtc cttctgactt
    76021 ttccgcttgt tttttctttt tcaagtaaat aaaacttgag agaacggtaa tgagtgaacc
    76081 aagaaaatag atcatttttt ttatcaccaa atcactcctt tatgagaaaa taatcgcata
    76141 gacatattct ttcttcttta tttcattgta ccgaataaaa gctaaaattt ctctaagact
    76201 ttcgactggt ttttttactg tttttctaca aattatgata ggatacatag taggaagaaa
    76261 ggtgacataa tttgaagtat cagcaagtgg aaatagcttt ttttattgac cgcccgaatc
    76321 ggtttatagc acattgtatg aaaaaagacg gagaaataat tattgcgcat gtcaaaaata
    76381 caggtagagg aaaagaggtc ctgataccag gcgctgaagt ggctattgta tttgctcctg
    76441 gacctaaacg gaaaaccgcc tatgatctga ttgctgtaaa aaagaagggt gactggttta
    76501 atatcgatag tcaattgcct aatcggctgg caattgatgg tattttagac ggcacgatcc
    76561 ttttaccagg attaactggt ccatttacgg tatacaagcg tgaagtaact tatcttcact
    76621 ccaaatttga tatttattta gagacggtgg tcggacaaaa agcatttgtt gaagtcaaag
    76681 gtatgacact tgaaaataaa gcgattggtg cttttcctga tgcgcccaca ctcagaggat
    76741 tgaaacatat aggagaatta acgtatgccg cacaagatgg ttatgccgct tatgtcttgt
    76801 ttattgctca gttcgaacat cttcatctgg caacgatcca tgaagaaatg caaccagcac
    76861 tagctgatat ggttcgacat gcgcagcaaa gcggagttca aatcctcgcc tataattgtc
    76921 aagtatcggc agatgaggta actgtcaagc aggccattcc atttgatgta gatatccctt
    76981 ttgaagatcc tatacaatag aaagaaggta aaataatgat caacttaggt attatcggaa
    77041 caaattggat tactcatcaa tttgtccaag cagctttaag cacaaaaaaa tatgaattga
    77101 cagcggtata ttctagacat ttggaaaaag cacaggaatt tggttctcat tatgaagggg
    77161 atatcgaatt cgcaacagat ttgaagactt ttttcaatat tactcatatg gacaccattt
    77221 atattgcttc tccgaatagt cttcacttta gtcaagctaa gcaagcaatt ttggcaggaa
    77281 aaaatgtgat agttgaaaag ccagcttttt cgacgccaaa agaaatggat gaaatcatcc
    77341 atttggcaaa tgaacacaat gtgtattttt ttgaagcagc aagaaatatc catgaatcag
    77401 gttttaaaaa gattgccgat tacttaccat taaaagacga gataatcggt gcaaatttca
    77461 gctatatgaa atattcttcg cgctatgacc aagtattaga tggagaagaa ccgaatatct
    77521 tctcgactca tttctcaggt ggtgcattga tggatttagg tgtttacctt gtatatgcag
    77581 ctgttgcttg gtttggaatg ccaaaagaag tccattattt cccaagaaag atttctactg
    77641 gtgtagatgg tcttggatgg gggatcttac gttatgagaa ttttgatgta tcgatccagc
    77701 caggtaaaat cggtgattct tatttaccgt cagagattta ttttgactca ggaacgctag
    77761 tgatgaatgg agtcaatgca attgaacaag cacaatttca tgatcgcgcg cataaagaaa
    77821 tcattgattt ggcggtaaca gctgcagaaa atccaatggc agatgaagca gaggattttg
    77881 ccaatgtcat ggctcatccg aaagatccag cttggggatt gttgtatgaa gagtggatcg
    77941 agctagctag aaatgtgaac caagtgattt atgatttaag aaaaaatggc ggcatcaaat
    78001 tcgatgcaga taatgaggaa gatttttaat gaacgaattt gtacaagtat ttcccgaaaa
    78061 ttggcagaaa aaatatgagg ataaagggtt tatctcacct tctcttattc aacgagcagt
    78121 ttttcagcca ctaagtaata aacaaagtat agtagcggtt tctcctacag gttcaggtaa
    78181 aacactggct tatctttggc ctctactact aaacgtggaa ccaggtgaag caagtgcgtt
    78241 ggtcattttt gcttcttctc aagaactagc tattcaagta gcagatgttg cacgtgaatg
    78301 gggaaaagac aaagagttga aagtccaatc tttggtgggc ggagccaacg tcaaacgtca
    78361 gattgagggt ttgaaggaaa aaccagaaat cttgattggt acaccaggaa gaatattgga
    78421 attgatgaaa gcaaaaaaaa taaaagcaca tcaagtgaaa acaatggtat ttgatgaagc
    78481 agaccagcta tttgacgctg ggaacagcca aatcattgat caaattcttc atcaagctcc
    78541 aacagagtat cagctagctt ttttttcagc gacagcagat cgttcccttg aaagcattga
    78601 aaaaatcact gggaaaacgt ttgagacaat tgacgtgaca gcagaggacg attctcgaaa
    78661 aggactgaga cattactttt tacgtgtacc gatacgaaag aaagatgact acttgagacg
    78721 tctcacgcat atagaagatt ttcatggtct gattttcttt aatcaactga ctgaattagg
    78781 cgtaatggaa gaaaaattgc tgtatagagg tgtaccggta ggaagtctgg cctctgatca
    78841 gaacaagcta ctccgaaaag cagcgctaga acaattcaaa aagaaaatgg tcagagcact
    78901 tttgaccaca gatgtggcgg ctagaggttt agatattaca ggtcttcctt atgtagtgaa
    78961 tgcagaagtt ccgttaagtg aagaagctta tttgcatcgt tcgggaagaa ctggtcgaat
    79021 gggtaatgaa ggaatagtaa ttacactcgt acaagataat aatctaagag atttaaaacg
    79081 gcttttacgt ccatcaaata taacacttga agagattttt ttatacggag gtaagctgca
    79141 aactgagcag ccaaccaaag aagatgcagc tcctgcagta aataaaaagt atgcgaaaac
    79201 tttttctgga aaggagcaga acccagaata taacaaagaa aaaataagaa aaaaagttaa
    79261 aaataaatca aaaaaagaga gaaataaagg agcaaggcgc aaataagcgc ctttttatgc
    79321 tatgattatg aaaacgggaa caaaattggt tgccagagga ggggttatgt gaaagaacgt
    79381 caagaagaga tcttgcagct attgacagaa aaccaacaag gattgacagc aagtgatgta
    79441 gctgagcgct taactatcga tcgaagcaat gcaagccgat atttaagtga actatacaag
    79501 gcccatcata ttgtaaaaac cgctggccga ccagtcgttt actctttgcc aacggaaaag
    79561 tcaaaatctg atgaagtgca tgtcgactct tctacgcaag tgacttttga aacactcgtt
    79621 ggagaaaatg attctttaaa agtcagcata cagcaggcaa aagctgcgat tctttatcca
    79681 ccgcgggggt tgcatacgat tatttttgga gaaactggta cagggaagtc gatgtttgct
    79741 gaatgtatgt atcattttgc aatcgattca gagatgcttt ctgcagatgc gccatttgtt
    79801 tctttcaatt gtgcggatta tgctcaaaat ccgcaactgc tctttggaca catctttggg
    79861 atcaaaaaag gggcttacac aggagcagct caagatagtc ccggtctaat agccaaagca
    79921 gacggcggta tattgttttt agatgagatc caccgtttac cgccggaagg gcaggaaatg
    79981 ctgtttactt tcattgataa aggtatttac cgtccgcttg gagaaagtgc acaagtacat
    80041 gaagcatctg ttcagatcat tggggcgacg actgaatctt cagaaagttt cttatctacg
    80101 ttcaatcgac ggattcctat ggcaatcacc ttgccgaatc ttgcttcacg ttcactggat
    80161 gagcgttatg aaatcatctc tctttttatc aaacaagaag cgaatcgttt gaatcaacgt
    80221 atcgatatcg aaaaagaagc gattcttgcc ttcatgcttt atgatgcgga aggaaatatc
    80281 ggtcaagtga aacgagactt gaaactagtc tgtgcaaaat cttttttaca ttaccgtact
    80341 catcatgaga aaaagctaat cattcgaaaa gaagaacttt cccttcaagt acaaaaagga
    80401 ttactgaaaa tcaaggaaat accggaacgt ctcgaccgat tcatggacag caaaagtcag
    80461 tatttgacat ttgaaccagg atttgctgat gttgtctggt cacaagatcc agaacgaaac
    80521 atgcaggtct ataacgatat tgaggaaaaa gtcacttctc tttccgaaac cggtgtggag
    80581 aacattgatc tagagaccct catttcaaaa gatgtggatg cttatttcca gacatatgtc
    80641 aaagaattga ctaaaagttc gattcccaaa gatcttttgc cagatgaaat ctggcggtta
    80701 accaatcaat tgtatgatgt ggctgaaaaa cggttagacc gtatttataa tgaaaaagct
    80761 cgttttgctt ttgctttgca tctgcaaagt acattggatc gtgtgagaga aggtcatatg
    80821 atcgtccacc cagatttgaa taacgtacgg aaaaatttta agagggaatt ccaggtagca
    80881 atcgatcttt caacgatcat tgaagaagaa gaacaagttg agattccttt tgatgaaatc
    80941 ggctttatta gtatgtttct ttctatcaat ctaggagaaa gtgagcctgt taaagaaaac
    81001 aaagtagatg ttctcatatt gatgcacggc agacagactg cttcaagcat gttggagatg
    81061 gcacaagaac tattaggaac aaaagtaggg aaagctttta atatgccact ggttatggaa
    81121 gttcaaacaa tgtatgaaga agtgttacaa tatgtcaaaa actatcaaga gcatttgacg
    81181 aatggcctac tcttgttaac agatatggga tcattaaaca ccttcagtga tttgatttat
    81241 gaagagactg gcattcggac gaaagctatc acgatgacaa gtaccatgat tgtgatagaa
    81301 gcgatacgga tggcaagtgt tggaagaagc ttagaagata tttatcaaaa tatccagctt
    81361 tcctttgaat cgatcgtacg tgatcagttc aaagcggaaa agtctgaaac tttacagcgt
    81421 aaaaaagccg taatcgtgac atgctttact ggtgaaggag tagctgccaa attatatcag
    81481 cgtattatgc cagtgattaa tcaaaataag gtagaactga tccagatgca gtttttagaa
    81541 cgtgaatcat tcaaaaaaca catcgatgcc ttattggaag aatatgagat caaagcaatt
    81601 gcgggaacag ttgatgttga ttatcaaaat atcccatttt tttcagccta tgacgttttt
    81661 gatgatgaaa aactaaatat tttaaagcgt atagctgatg atgagatccc gacagctaaa
    81721 attgtcgatt cacttgctgg atcattaagc gatgtctttt ctattgatga actgatccgt
    81781 tcattgcaaa agacagttca gcagatccag accgatttgt cgattatcgt ggaacctgga
    81841 gtagatactg gaatcgttat ccatttagcc ttcatgttag acggattgat caaacaagaa
    81901 agccatcggc aatttgagaa actgtctatt ttctcaaaaa tttatcgttt ggaaatggat
    81961 atcgttcggg caaacttgat ggcactagaa cgaaaatatc aggtaaaaat tccagaagat
    82021 gatatcgcat ttcttacaca aatgtttctt gaaaataaaa taaaaaaatg aacacactaa
    82081 tcatactgtt tacacactaa acatctgtgt gcgcactata aaaaagagct aaaaagcatg
    82141 aaaacgctat ttcagctctt ttttttattg gcatgcttct tgcgttatat aaaagtgtaa
    82201 aaaaataaaa ggagtgttaa aaatggcaaa aaaaacaatc atgttagttt gttcagcagg
    82261 gatgagtacg agtttattgg tcacaaaaat gcaaaaagca gcagaagaaa aagggatgga
    82321 agccgatatt tttgcggtct ctgcctcaga tgcagataat aatttagaat ctaagaatgt
    82381 cgatgtatta ttattaggtc ctcaagtacg tttcatgaaa gctcaatttg aacaaaaact
    82441 tgcgcctaaa ggtattccat tagatgtcat caatatgcaa gactatggca tgatgaacgg
    82501 agaaaaagtt cttgcacaag cagaaaatct aatgaaataa atcaagtatg cagttggggg
    82561 tataacaacg tggaagatca acaaaatcta gaagccatca tggggttaat catgtttggc
    82621 ggtaatgcaa agagcgatgc aatggaagcg attgcggctg ctaaaatcgg tgattttgag
    82681 ttagcggata ggaaaatcgc tgatgcagaa gaatcactta tacaagcgca tcatgcacaa
    82741 acagacatgt tgacacaaga agcacaagga aatcatatgc aagtgacatt attgactgtt
    82801 cacagtcaag atcacttaat gacatctatt gcgtttactg atttagcaaa agaaatcatc
    82861 gaactttatc gtcgcgtata cagcgaataa atacaacttc ttttttgaac ggaagaagaa
    82921 aaatttttga acggattttt tattttgtta taaggagaac tctctttata taaaaaactt
    82981 ataggaggaa gtaaaaaatg aacggactat caagttggat ggagaaatac atcctgccgg
    83041 ttgccgcaaa aatcggagcg caaaaacatt tagttgcttt gcgtgatgct tttatcggca
    83101 cactgcccgc aacaatggcg ggatcagtcg ctgtaatgat caatgcaatt attcgtgatt
    83161 taccgcaaca gtttaacagt acctatgctg acagtttagc agcagacaaa ggaatctttg
    83221 ccgtattgaa tgtcatcatc ggaatcaatg gttttgtctg gaacggtaca ctagcaatcg
    83281 ccggattgat ctttgccttt tcttgggggt ataatcttgc ccgagcttat ggagtaaatg
    83341 atctagctgg ggggatcgtt ggtttagcta ccttgattca aggtatttcc tttggtccag
    83401 cattatcagc agcattagat gtgaatgttc cttcaaatgt cgcagatgca atcaatagtt
    83461 ctgagattgg tgcaacgatt gcagatggca cactgtcagt tggattatgg ggttggctaa
    83521 aactagatca tttgaatgga aacgcttatt ttacagttat gatcatggga gctatttcag
    83581 tcattatttt ttgtaaattg atgcttgcca acattacgat caaaatgcct gattcagtac
    83641 caccagcagt atctaaagca tttgcagcca ttttaccagc gacgatcgct ttatacgtca
    83701 ttgctattat caattatatc gtaggtcgtg tagctgatgg gcaactgatc attgatttgg
    83761 tgcaaaaata tattgcagaa ccattcctag gattatctca agggttggga gcagtattga
    83821 tcgtcacagt atttgttcaa gtattctggt tctttggtat tcatggacca aatgtattgg
    83881 caccagtgct agagggaatc tggggacaag cacaactaat caatattgat attttccaaa
    83941 aaggttataa cggaaaaact ggtacaccag ccgtgttaga tgcaatcgaa gatggaaaag
    84001 cttatatgtg ggtccgcgga tcgttcgatg cttttgcatg gtttggcgga tcaggcggaa
    84061 cgatcgtctt actgatagct atcttgattt tctctaaacg tgcagattat ctaacagtag
    84121 ctaaattatc cattgggcct ggtatcttca atatcaacga accagtgatg tttggtctgc
    84181 caatcgtatt gaatgcaatc atgtttatcc cattcttgat tgctccagta gtagcaacag
    84241 caattggatg gacagctact tatcttggcc tagtcgctcc ggtatcgcag caagtcacat
    84301 gggtagtacc gccgttctta ttgtcattct tagcaacagg agcggattgg cgggcaccga
    84361 tcgtaacatt agtatgtatg gtcgtgactt tcttgatttg ggcaccattt gtcatctctg
    84421 caaacaaaat ggaccctgcc gcaggcgaac aacaataaga tgaacgtgag gagcgggacg
    84481 ctttaagttt cgttcctcat aatgaatttt cgaggaggaa ctgaaatgat tacaattgcc
    84541 agtgcaatgc aacaagatga aatcaaagaa ttgttagaag gatacaatga agaaggaatg
    84601 acttttacat ttaaagaaaa acaaggaatc aagctgttct ttgaaacaag tgcagaagat
    84661 ccggaagttg cggctaaaaa agcaaaggaa ctaatcaaag cacagccttg gggaacagtc
    84721 ctttattttc aatcaacagc tgtttagaaa atggtttaaa aagataggat ggttgagatg
    84781 ataaaaaacg gtttattttt atgtagtatt ggttctttga tcgtgttgta tagtttgaat
    84841 ccacattcgc aaaggtatga tttgtatagt atggtcacag gtctttttct aacaggatta
    84901 ggaatctatt tcttcatgaa ggggaaaaaa agagaagaag caaaggagaa aaataactaa
    84961 tggaaatcac gaaaaaatta aatattccag catcgttttt ttacgataaa gttatggact
    85021 ctgtcctttt cgatgtacgt aaagcgaccg gcaaatcagt aactagaaaa cagctgaaaa
    85081 atttggagta cgtcaaacaa ttctcgcaaa ataaccgggc tcgcataaaa atcgaagaag
    85141 tcattgaaaa ccagtcttat aagtttcgta catcgacaac gaagaatgaa ttcgtcgttc
    85201 attatcaaat caaaccattg gacgattcga cctgtgaggt tcattacaca gaaaaaatgg
    85261 agtcttatgg catgcttcaa aagttgaatg atatgctttt aggaacaatc ttgatgtatt
    85321 ttaaacggag acgtttcaaa aaaatgctag atatgatcga agcaacgtat taaatcaatg
    85381 aaacatggta ctatttagtt attgaaaaat gaagtaagct agaaggaatt tttcatagga
    85441 agatagatct ttcctgcgaa tcctcactag cttttttgta ggaaggggca gcaaaatgac
    85501 aaacaaagta agagagctag gtgtatcttc gattgaggaa atgtttgatt tagccactta
    85561 tgcatttaat ggggcagata cacctgaaag gcgagaacgt tttcgtacac ttgcggagaa
    85621 ttcttggaat tacggatttt ttgatgagga ggatcggcta acaagtcaag tcatggcgac
    85681 cccttttcca gtgaattttt acggacaaga gtacctaatg gccggcattg gctatgttgc
    85741 ttcttatcca gaagcaaggg gacaaggcgg gatcaatcag atcatggaaa agatccttga
    85801 agattgccgt gatcgtaaag taagcttatc ttatctggcg ccattctcct atcccttcta
    85861 tcggcgctat ggctatgaac aatcatttga taaaatcagt tatcaactaa acagtcgtga
    85921 tgtgccgttt atcaaaaaaa aatctggaaa aataaagcgt atggattttg aagatgcaaa
    85981 agctgcaatc agaagaattt atgaacagat gccggaaaac caacgagctg gtctgaagcg
    86041 cgaagactgg tggtatacgt ataaattcaa gcaaaaaggc aattaccagt ttgctttgca
    86101 ttttgatgat aatgaggaag caacgggata tatcgtttat cagcttgcaa cgtcttcctt
    86161 tattattgcg gagtggggat ttttggatca tgatgctttt tgcagccttg ttcgcttcgt
    86221 aagttctcac aatggtgctt ttgaaacatt tgaatattca tgcggctacg gcggtaatga
    86281 tcaaaattat ctattagaaa atccatttgc ttctgtaatg atcacacctt atatgatggc
    86341 gaggatcgta gatatttctt tgtttttgga aaattatcca tttaaaaaga gacaagctgc
    86401 atttgcccta caaatagaag aagatcatta tgcgaaatgg aatgaaggaa tcgtggaagt
    86461 aacaatcgaa aatggaaaaa atcatctctc aaaagtagaa caaacaaaac tcccaatcgt
    86521 tcgaggaagt atccaacata tcacgcagct attattaggg tatcgtaaag tggaagagtt
    86581 ggtattccaa gaaagaatac aggtgacaaa agaatatgaa gaaacgttaa gctcaatctt
    86641 gccacagcaa cagccaattc ttaatgacta tttttgataa aaaacgggtg atgacaatat
    86701 ttttgtcacc acccttttgt ttttcatttg gcatttgaag gaaaatgtta aagtaaatcc
    86761 tcttttttcg taaatttgaa ttttttgttt tcttttgttt tagggttttc ttttttgtgg
    86821 tcttggattg cttctttcgt aatcttttcc cgattttttt tagatgaatc taagcgttct
    86881 gcgtatactt tttcttttgg cttatcattc attggaaagg acctccctac attatttcat
    86941 tctacctcta gtttatcatc tgttaaggac tagtggaaac gttttgccta acttataagc
    87001 tgattttttt agcagataag cttgcaagta aaaaacaaac aagctacaat tgatttattg
    87061 aaattgttcc tgtagctcag taggatagag caatcgcctc ctaagcgata ggtcgctggt
    87121 tcaattccag tcaggaacgt ataaataacc cctgtatcct ttgcggtaca ggggtttgtt
    87181 ttgtatttgt gccaaaaatg tgccaaacta gttatcattt ttatataaac tatcgaacac
    87241 agtattgatt ttttctttat cctgatcttc aagctctcga acaatatgaa gataagtaga
    87301 cattgtagtt tctaaactgc tatgtcctaa acgtttcgat acgctaagta tatttactcc
    87361 ttgataaagt aaaatagatg catgtgtatg ccttagtcca tgcaaagtaa attgcttttc
    87421 aatccctaat tcttttaatt ttttgcgcaa atatttgctg acggcgtttg atgaaactaa
    87481 tccatttttt aagttgaaaa aaacaaaatt atgagggttt ttgatttcaa aattctcgaa
    87541 taattctttt tgattgattt taaacttttt taagagatca atcgtatgtt tatcaatgga
    87601 aattttccta tttgaagttt cgttttttgt ttttccccat tcatttaatt tataattcca
    87661 tgttttatta atagtgatga tttgatcttc gaaatcgata tcttcccatg ttaatcctag
    87721 aagttcagca aatcgcattc cagtggcacc agcaactaac acgagcatag gagaagagta
    87781 ctgcgcagaa aggttttctt ctacgagttt cattaaagat ttaaattgat cataatctaa
    87841 atatttatct tcttttggct tcaatgaatc ttttcctttg attactgctt ttctagttgg
    87901 atcaaacgga attaatcctt cttctacggc atctttcaac gacgctctaa tatggttatt
    87961 aaacttaata acggtagatt ttacatggtc tttagcatac ttatttaaaa agcgttgata
    88021 tcccactcta tctaaatcag aaatcaatac cgctggcata tatttcttta tgttcattaa
    88081 cgtatcttcg tattttctat aagtgatagg agatactgtt ccttctttat aaagttgcat
    88141 ccaatcctca aagtaatctg ataataatag atttttgcga tcccctttaa ggcctttgtt
    88201 caagttatat tctaattcgt tggcggcatc ttttgcttct ccttttgtct taaatcctga
    88261 ttttcttatc ttcgtgtatt ttccgtcatc ttttttgtat gaaatttcgt attgccaact
    88321 atttccacgt ttgactaatc ttgccataat tgaatcagct ctctttctct gatacaatag
    88381 gaactataaa gaagcccatt gtttgggttt tattattttt tgcacatcct ttttgcttgc
    88441 cggcgcaagg atgtgctatt tgtcgtttta atggtataat tggatgtgta ttagatcatc
    88501 caatcagagg tgtttattat gattaaagtg atttttaaaa gaataactga acttccagag
    88561 tttagattat ttgttgatta ttggtatgtc tggtttttta ttgttgcatt gctgataggt
    88621 ctagatgtca tattagaaag aaaacttaag taagagttga gttaataaat tgaattccta
    88681 aatgagatat tttgtagccc atattttgtt tgccgacatc acctaatact atggagctaa
    88741 tcgttttttc taacagacca aaatcagata atctatttaa gctaccatct attagctctt
    88801 gtgaatagcc agacatgctg atatttccag tgaaattttc gtttccacga ctcattaagc
    88861 atgctaacaa ttgaatctca atcgatgtta attgattcag tacaagaaga taatactcct
    88921 cgttatccca tgaagcgttg ttaattcgaa gtaaacaatt cttataaaga tttttataca
    88981 tatctatttt attttgtgcc tttgattttt ctacctcgtc atttatccct tcgaatattc
    89041 caatgagttg gtccttgttc tctaagttga aaacatcttc tggaatttga tcgcgaatgt
    89101 tttctaatga ttcgtttaaa tttttgtaga atttctcgat gcgtttgaaa cgcttctcgt
    89161 ttttagaccc aaaataaagt gactgcacaa cgccaccaat ataaggaatt gctccaatac
    89221 cgccttcaac tattaattca agatattctt ttttggttag tttttcattg ctattatcca
    89281 tagttacctc ctattttgtc atttccttta caagcattgt gactcctgat ggcactcttt
    89341 tgtataggtt ttattattgt gcacaatcta actcttagtg gggcgtggtt catttatttt
    89401 ttacttactt tactattaat catatcccat tgagcatagg atttttctat gttcgatcct
    89461 cttgaactaa tttctcctgt aaaagttact ttatcgccct ttgtaaaggt atcagcattg
    89521 tttaagtcct tcgcaaaaat tacatatgaa actttaggtg ttgcagaaat atcattccaa
    89581 cttttatcat cgtactcttt gtctgctata atggctattc tagtagtcat tgattcgata
    89641 acagttcctt caaaagtata tgttttacca ctaatatatt tagtaaaagc tgtattttgc
    89701 tcagtagctg gagtaataga ataataacta tctaagaaag caccccagtc atgattattt
    89761 gtctctaaaa attcttcaaa ttgagttagg cttttattgc tgctgttaga tgtagtagtt
    89821 tgagtatcag atgatgagct ttcctgtttg ttattgtcat ttgcctcgtt tgatgaggaa
    89881 agcgagaaac ctacggtagt aaaaataatc ccgataataa ggagaatcat aggtgttttc
    89941 ttatttgttt ttttgataaa tcttacaatt aacatgatta aacctataat aaataaaaca
    90001 ataccagtaa aaccaaatat accgtccatt aatagatcct ctttctcttt tgatattgaa
    90061 tattataaac caagcaattc ttttttctta aaatcgaatt cttcttgatt aataatccct
    90121 tcatctaaca actctttata ctttttaatt tcatctgcac ttgaaatttg tgtttctttg
    90181 gaattattat ctaatataga ttgttgcttc tctatatatt tttttaaatt ttccatttca
    90241 ttccaatatt tttttgtaaa cataacggtg ttttcatctt tcgtggccgc taatatacct
    90301 ccgcgacttt cgtttccgcc tagcaaggta aattgtatat aaccattact catacctggt
    90361 tttttttatt gtacggctga tatattttta aaggggatag ttttttctcc tttaatacct
    90421 tgattaacta aatttataaa tccttttcta ataattgaaa tagaatcatc agttacttta
    90481 atcaaggttt ttcctggaga ttttattaaa atctcttttt ccattttttc ttcctcattt
    90541 ctgtgatatg attttttttg tagaagctca tatatgagga aagagcccgt gttgcagcac
    90601 gggctttttt aatgttttgc tgaaatcggt ttttttaata attcttgatg tattcataca
    90661 tattgccttg cgtaagaata ttttttctta aaatagcatt ggcagacagc ataacaagtg
    90721 tatctgagct tattctgaat agaataatac tccataaatt tttcgagatt aaactgagat
    90781 tcatcagtca gttcgttctc aatatagata tttaataaaa ttagaatagc tattttatct
    90841 gcttctgttt caaattttga gtgaaaagtt gtagaagtat cgtacaaaac tgaaaattca
    90901 aaatgagagg cgctgaaatg tgcaagttca tgagataaat gaaaggcttc tgcagtttca
    90961 ccgtatagat tttcattcaa aaaaatgatt ctgggttttg gatagtagaa accaggctct
    91021 ttcatctcca tataaactac tttcaaattg tattcgctca acatttcttt caatttcaaa
    91081 tacatacaaa ccatcactcc aactattcat tttcctctaa agctttagca attgcaatcg
    91141 ctttacgcat tgtctcctta gatatttctt ttccatcaaa agaaaaaaca gtatcgtctt
    91201 ctgataaatc cacatgttta ggggtttctc tttcttctct acctagaagg tagtctacag
    91261 agacatcgaa atagtcagca atccgactta attcatctga attaggagta ttatttttcc
    91321 actttgctag ataaccgtta gaatatccaa aattaatttc taactgccgt atagatatct
    91381 ttttcctctt cgccaagtct tttattattt cataggtatt catcgataaa acaacctttc
    91441 taaatgttta caaaaaaagt ttagaaaaat acgcagaaat aatttgacta attctgagta
    91501 atgagctata ctatatcttg taaacagatt taacaactaa aaagacaaca aaaaacacta
    91561 ttgatatgta aatgcagacc gccaagaaag ctttaaaatc aatgttttta tgtcttattt
    91621 aattatgctc ttagtataga gtattactca gatactgtca attgaattta gaaaaaagtt
    91681 gttaaatttg tttacgaata taaaagaaag gaatgagaaa tatgaacaca ccgcaaattt
    91741 ttaatttcga acaaaacgaa gttcggacaa ttttagtaaa tgatgaacca tattttgtag
    91801 gcaaagacgt tgcgagtgtt ttgggttatt caaacactaa agatgctttg tcgcggcatg
    91861 tagatttgga agataagatg gggtcgcgaa tcacgacctc aggtcaatca agagagatga
    91921 caatcatcaa cgaatctggt ttgtacagtt taatcttaaa atcaaaactt ccctctgcca
    91981 aaaaatttaa acgttgggta acaagtgaag tgttgccagc aattagaaaa catggaggtt
    92041 atctaactcc agaaaaagta gaagaagctt tgcttaatcc agatacaatc attcaattag
    92101 caactcaact aaaagaagaa agaactggaa gattaatcgc agaacaaaag attgcagagt
    92161 acgaacctaa aatctcctat ttagatagca tattatcttc tacagattca gtaacaatta
    92221 gtcagattgc agcagattat gggatgtctc cacaacagat gaataaatta cttcataaac
    92281 taggtattca gaaaaaagtc ggtaaccaat ggttattgtg caaaaaacac atgaaccaag
    92341 gatacacaaa atctcataca actgagatcc cgaaagccga tggtggcact aaaattgtaa
    92401 tgaataccaa atggacacag aaagggcgtc tatttatcta cgaattacta aaaaaagaag
    92461 gatattaccc tcaaatggat ttagaggaaa ttggttagaa aggagtttta gtatgactga
    92521 cattgcagaa atcactcaac gagatagaga aaaaatcaaa gaatatgtcg aaagttcaaa
    92581 gttcttaact tacaccatgc ttgctgaaag atttggaatt agcaaaagct acttatcttt
    92641 aattttaaac ggtaaaaaga cttctgcaga agcaaacaga attatagatt cgattatcac
    92701 tatgtacgaa ttgtaaaggg aggaacagct aatgcaatat ctagaagcaa aaatcccaat
    92761 tccagaaggc tatgtaatta tctcccaagt ggattatgag gagttaaaaa aagctgatga
    92821 tactggtaga tggatgacgt tgccagaagt gctagaacgg attaacagaa aatatgattg
    92881 gtttacttct agagttttaa agaacccaag atatagaaat attatcgata tcgagaaaaa
    92941 caaaaatgga tttgtctatt atccagttga aggaagagac acatatctat ttttaagaag
    93001 taaaacactt gaatttttag aaacaaattt ttcggaaatc ttaaggaggc aagcggatgg
    93061 gaaaatttaa tagagcgcta gtgttcagcg caccgctaat catctacgct ttaggacttt
    93121 ggggaagcag gcaagcgttg ataggaacga ttgtttacat ggtttggatt tttatggggc
    93181 ttgatgaagc tgaagctgag tacagagcga aaaagccaac cgagggggct gactaaaaat
    93241 gaatagaaaa gagaaactag aatgtatatt actattactc agtttaattc tttcactaat
    93301 ttctctattg ggaagtttgt atttttgata tcaacaattt tgtggatttg ggttctattt
    93361 agagtaaata tcatttttgt ttggtgatta aggagtttat taaaattaat tttccctact
    93421 tcaaatgcaa ggtaacaaga tatagcactt ttaggaggaa tatttagcgg tacagcttca
    93481 gagtatattc tattatcatc gactgatcct cctttagcaa ttaatttctt aaatggagta
    93541 gctgaccatc tgcggtttaa gttagcggat gaaagttcta aattaactaa catagctggt
    93601 tctgttgaat agttagaaat gattactttt gtataaatca tatctgcagc gaagtaagaa
    93661 gcattaagtt caacttctag ttgagggcgc attcctttta tttttatccc tgttaaaaac
    93721 gtactcaata taaaacttat tattgacatc cattgaaaaa ttgttaagtg aaaaaaattc
    93781 aaaataaaca ccaccagttt ttaactaaat tataccaaaa aggagagaag aaataatgca
    93841 agaattagta attttgaaaa ataaagaagc tgtgactacg agcttacaag tggcagaaag
    93901 cttcgagaaa aaacatcaac atgttttaag agatattgat gcactaaaaa aagatgtgtc
    93961 caattttgga cagatgtttg tagaaggtaa tgaaccagat tcatatggca gaaatcgacg
    94021 agttttcttc attagtagag atggattttt cttgctggct atggggttta caggaaagaa
    94081 agctatctac ttcaaacaaa aatacattga agcattcaac gaaatggaag atgttattcg
    94141 caagaatact gttcctcaaa caattgaaga tatgatgatc tatcaattag aagaaatgaa
    94201 agatgttaaa aaagatgttt ccatgcttaa agatactatg cgaattagcg gacaacaaga
    94261 gtttgaaatt aagcaaaaag gaaatatgaa agttatggga gttctagggg gaaaagaaag
    94321 ccgagcttat gaagaaatca gcaaaaaagt attctcaaaa ttttggtctg aatttaaacg
    94381 taccttttca atcccaagat atggcgagtt acctcgtaag agattcgatg atgctgtttc
    94441 atttattgaa atgtggttgc cagaaactgc gatccgtatg gaaattgatc aactgaacag
    94501 acaacagaga ctttttggtg atgacaatga atagagctga agcgctaaga atagggacgg
    94561 caattgctaa ttgctggtgg aaatactata aaccaatcat cctaagccaa caacatattg
    94621 acaagcaaaa agcatggcaa caaataaaaa agtgactccg ccggcaagca aagagtcaca
    94681 aagaaaacac atcataagga gattttagca tatggaaaaa gaactttcca ctctagatca
    94741 atatttgatt gatcctgatt ggggcaagcc gaaaattgag gaaacaagtg gtcgaaaaat
    94801 cagacgaaat cttttgacga atgaagaact agcttgggat caagatgatt taggcaacca
    94861 tgtaactatt tgggatcatg tttatcttat ccatctatcg aagcattcga ataaacctga
    94921 atatatttac gtcatcgaag atggcttgat tgatgcgcta gaagagtacg acagagataa
    94981 cttgattgat atctcttatt acggaccagg taagaaatac attgctgaaa tggaggcaga
    95041 atttgatgag tgaaatcaaa gggacaacga actttgaaaa actttttagt cgtaagttaa
    95101 ataaaattct caagaaaaaa ggaaattttg attatttatc ttgggctcac gcgtgggaga
    95161 ttatgaaaaa gaatgatcca caggcaacgg taactattaa tgagtacaaa cactacaggg
    95221 ttgtttctgg aactcatcaa gactttcttg ttgaggaata taaacctttt cttatggatg
    95281 aaactgggac ttatgtatct gtctcagtaa cggttaaagg acacacggaa accgaattat
    95341 ttcctgtttt agattatcga aaccaaccag ttgttaaacc aaatgcaatg caaatcaata
    95401 actcattgaa gcgatgcttt gtgaaagcat tggctctaca cggactggga ttatatgtat
    95461 ttcaagggga agatattcca acaccaccta gaatcgatac aaagaaatta aacatgctag
    95521 agacgattct agaagctttc aatgagcaga tgggtaaaga tatgaccaaa accttaatcg
    95581 aatatgttaa tgagcagaca gataaattag ggctcttagc tgataacgtt gaaactattg
    95641 aacagttaag ctatgagcaa tgtgccttga tggagcgagc aatagcagct aagagaaaag
    95701 aattagataa gaagtgatat gagtgtttaa accattaatc gattcatatt cagcggttct
    95761 gaaaaagttc aaaggaaaag acataagcgc aaccatcaat gaggaagtga acattgatcg
    95821 actaaagacg atgtatgacg gctacgatgg tgatcgagtc attgaaattc gttttattga
    95881 tcctagacgt ttcaccgtac agcaacgaaa cttcatctat gcgctgatag gcgatatttt
    95941 tatcgataca ggcatgccaa cggacttctg gaaggaattc ttctactttc gttttgaagg
    96001 tgtcacaggg cgcaaaataa gcctgaaaga cgaatcgaat acaactgtga gtgatgccaa
    96061 tgtcttagca aatatcatct tagatttcat ctttgaacat catattcctt tcaaagaagg
    96121 ctatgagatt ttacctgcga atcaagaata ttacttctac aaatgcatca caaaaagagt
    96181 ctgctgcatc tgtggcaaaa caggagctga catcgatcac tttgacaaag cgctaggaag
    96241 acgaaagcgc aaagaagttg atcatgcaga gtacacattt gcagcactct gcagaatcca
    96301 tcacacagag aagcacaaaa taggtgtgat taatttcaaa aataaatatc aaatcaaagg
    96361 gatcaaatta aaccagaaaa caatcaaaaa gttaaatatt ggagggtaaa aatgacagaa
    96421 catcgaagtt attatgcgat tataccagcc aacgtaaggt acgacaaaag acttaaacca
    96481 aatactaagt tgttatacgg agagataacg gccttgtgta atgaaagagg cttttgttgg
    96541 gcaggcaatg agtactttgc agatttatat ggtgtgaata aagagaccat atcgcgatgg
    96601 gtaagtgatt tgattaagtt tggatacttg aatcgggaaa tcatttacaa agagggtacc
    96661 aatcaaataa tcaataggta cctacgaatt aatcaatacc ctattgacga aaaacgcaat
    96721 acccctattg acgaaaaagt caaagataat aatacatcta ttaataatac atttaataat
    96781 acaaaagaat atataagaga gttaccgcct tcgaaaaaat cgaaggctaa gcccgtccgt
    96841 cataaatacg gagagtataa aaatgttctt ttgtcagatg agcagatgga gaaactcaaa
    96901 acagaattcc ctaatgatta ccaagagcga atcgaacgac tgtcagagta ttgtgaatca
    96961 tctggtaaga cttataaaaa ctatttggca actattcgaa gttgggcaag gaaagaaaaa
    97021 agtgaaccta agaacgcaag cagtggatac aagcgcacag gaagacgaga gaagcttcct
    97081 gaatgggcaa tcgaccaaga agcctatctc aagaaaaaag cgctagaacg agctaataga
    97141 caatcaaaag caccattcta agaggtggaa aaatgaagat cgattatcta gaactaatta
    97201 atgaaatagc gaattataaa aagggcgagg aattagacgt cctgagagac gtatatgatc
    97261 aactcgaaga agctggaatt gaaggaatta agaatgatcg ttcgagttgg agtaagctca
    97321 gatactattt cgcactttat atcgatacaa cacaattaag aaatttagca tataccaaat
    97381 tactatttgt tgattgtgtt aaaggcttgc aaaaacatct taatgaactt gagcaggtgt
    97441 aatcagatgg acctaaagac atttacagca cagattgaac taatgcatca agaagcttta
    97501 agacaaagtg tatcgtacga agacaagtgg ctcaacacgt tccatggcgg acgtgagagc
    97561 gcacttgatc aagtactcaa attactgaaa ggagaatgtc gggatggata agaaagcggc
    97621 aatgcagcga attatcgaat tgacttattc agaagattgg caaaatgaca aagaagctgc
    97681 ttcagaagtg atgaggcttg gaagagcgat gtgggcagac aagagcaaca agccaagacc
    97741 acgaaaaatc gcaatttggc acggtgacaa acttctagtg atagggacag ctgaacagtt
    97801 agcaagtctc acaggcttgc acgagaaaat cgtgaggaaa agagcaaggt gtggctacac
    97861 agacgctaag aagagaacgt ttagatacgt ggaggaatca tcatgacaac agaagaagtg
    97921 attcaaatgc gtattcgaag ccttcagcgt gagattgacg atctggaacg aacaaaggca
    97981 gtgatggtca atgaaacggc gagaaaggca atcgatttgc acatagagaa tttaagaagg
    98041 gaaatccatc gattggagga atgagcgtgg ataagaaagc agcaatgaaa agaattgctg
    98101 aattaaccaa gtcagaatct tggcaagaag acaaagaaat agttgcagaa gtccaaaagc
    98161 tcggtaaatc aatgtggact gaaaagccca aacggagaac gccgagaaaa attgcaatct
    98221 ggcatgatga ccgaatttta gtaacaggta ctgctgaaca gttatctgaa attactggat
    98281 taagcaaaaa cattatctgg gatagagcta ggagcttatg gattgattca aaaggacgac
    98341 agtttaggta tgtggaggag agataatgga tctcattaca caatacagtg acatcatcct
    98401 caagaaaatc atgatgaaga ttcagaaaga taaaaaatca aaagaacgag ctgaattagt
    98461 taagttagaa atggctgaaa caggagcagg agtgcgaagt agcaggcatt ggaaagcagc
    98521 agcaaacatt gaattttatt acaacgaaat tcaaaaaggg ttcgatcaga tgcgtgagct
    98581 ggatcggcaa acaaattgga gcaagaaact tcatcaagat cgtttcaaat ttgtagagaa
    98641 gtataaagaa atattagaag agtatttgag gaggacagca aatgataaaa aaactcgttc
    98701 aattcagcat ggatttatat gatatcgaat caggagcaac actatctgtg gaatcggacc
    98761 atctaatcat aaattttggt ggaaagcgcc agattatttt gtgggtagtt gatgatgtac
    98821 tgtttccaga aattgttcat gatttcgaag aatcaaaagc ggttgagttt gaaatagtga
    98881 aaaaagtaat ggaattgatt gaaaaatacg aggaggacag cgaatgatac cgaagtttag
    98941 agcgtgggat aaacgaaaga acgtaatgag agatgtagcc gtcttgcatt ttactaaaaa
    99001 cggcaaaaca aactttattg aatattggat aaatcctacc gaattgaaat catatcacgt
    99061 gcgaaacatc gacctcatgc aatccacagg aatgaaagat aagaatggtg tggatatatt
    99121 tgaaggggat atagtattag tcagcgtgcg aaatggcttc gattacttag ataataaagt
    99181 ctgtattgtc aaaaattcaa tagattattc cggattagtt tgtgccactg ttgatgaaga
    99241 cttagagtat caaattttta acacagagct gtttgaagaa tacacgtacg aagtcaccgg
    99301 aaatatatac gagaatagcg agttattgga ggaacagcga tgaataaaca ggaagcaatc
    99361 aaaaagttag aaagtattaa agcgatagga aatgatgcaa tagctgcttg ctataacgag
    99421 agtataaatt caggtattac gttaatgaaa aaaatagacg aaccgcagaa accagttatt
    99481 ccacaactcg tggccggttg gcttgagaaa tctacggacc cttttacaaa agctgaaaaa
    99541 atagcgtatt taatcaaatc taaagatggt gattcatatt atttctgtga ttggtttgta
    99601 cgagatggca tagtgacgca agagcaagga gaagaattac tcgcttgggc aacaagacaa
    99661 tcatatgaaa cactattgag cctatacaac ggctacgagg ttgagaaaga gccgttatgg
    99721 gcaataaaga atgccgatgg aaactatctt actaaatgtg ctttatgggg aaaagatgga
    99781 gtaaattata gttttgaatg caatccatct catcgattgc ttttcactga taaagcaaca
    99841 gcggatgctg cagcattgtt ggtgaatgga acagtggaag aggtggtaga aagatgaaac
    99901 taaaagacgg attttacgct agcagtcacg gtatcggcgg tttaatgcta gatatgccga
    99961 caaagaaccc taaaacacgc aagaaatcaa aattcaaagt cggtgacatg gtccgctgtg
   100021 aagcagaaga gttcgtttat ccgttcagag gatacgttaa aaagatactg tcaaactcag
   100081 caatcattcg tattgaaaat acaatgaagt gtgatcgttc gactgcaaaa cacaagcatt
   100141 atttagctgt agcgagatta gttgacatgg aagtaatcaa gagcaaataa aaaaagccgg
   100201 atcgctccga ctgattcaat aaatccaaca catttattat atcacataaa ggagcggttt
   100261 gacttgatgc aattgttacg agaggtagat ttcaaacaga caagatgtaa tgcgagagat
   100321 gtgctgaaga actttcggcg tttggagcgg atggcaggtc gctctttgat agatattaag
   100381 tcgccgatta taacggatat gccgaaggca ccgaagcatg gcaataaggc agaagacgcg
   100441 attattcaga tgatggatat agaaacggag agagatgcga ttctagcggc tttgatggct
   100501 cttagtctga ttagtcgtca gatactctac tacagcttct gtgtgccaga tagcttctca
   100561 aactacagaa ttagccgtga agtgggttat tcagaaagaa gtatacaacg gatgaagtcg
   100621 gaagctctaa tagagtttgc agaagcatat aaacacggaa aaataattgc ttataaataa
   100681 tttggcggtt ttttggcgga atgatggcgg tttttagaca tttaccagtg ttattatggt
   100741 agtgtcgaaa gattaggaaa caggatcgac aaaataaacg aagggaggca atctccctca
   100801 tcgttttaaa cttcttctta gatagacagc aactaattat gaagaaaagg atgtgaattc
   100861 aactcctcct aaattcttca tcggttgctg tttattttga ttagacagca gcactcacaa
   100921 aactaaacat gttcttgtcg tctttcaact gctgctgtct attaatttat gtattggagg
   100981 gaagaaaaat gaaattatca gtagaaggca ctccagaaga aatacaagaa ttgctccaag
   101041 ctattggtag tagcaaggag caaaaggtaa gtgtttctaa ttcagatatt gacggtattt
   101101 gcaattgttt aaacagagaa aaaatatcta atccttacta agagaaaata tgttcgttaa
   101161 tgaatttcca attttcctct gtagctctac cttgataatt tttttgcagt tcacagatga
   101221 ataaggaatc gttattatca agtactgtat ttaatttttc acacatttga tttggattca
   101281 aattagtttt aactaaccaa aaagattttt gtagctttat atatgcccca aattctttta
   101341 ttatttcaaa tactttatca tacttttgcc ctgggttatt aagatcataa ctaatcatgt
   101401 agggtttata catgttgtct tgcacatcct tgttttatat ttcagcggac cactcgctga
   101461 taaataaaat tatacgctta gtatttattt tcacaatatt aatttatcgt aataactttt
   101521 gagtaaatag aacaaaaaac ctgcactagt ttccgctagt acaggcagtg actatatcgg
   101581 ttgataatct agcatatatt aaaaataatt gcaagaaaag aaatttatag tggtttatac
   101641 caaattatca aagcaatgtt attttgttgc tgtctattgt tttttaatta ttcacacgat
   101701 aactaaaggt gggtgaagag aaatgattcc attaataatt tcaatttttg cgctctgtct
   101761 taatgtctat atgattggat ttaaaaatgg gcaaaataaa aaatagtagc agccaagaat
   101821 aattttatag tgtcactgtg gcggaaaggg tagacgctaa gcatgtgtgc taggtcaatg
   101881 cttcggcaac catgcaaggt tcgattcctt gccagcgaca ttaaatgcct atgacggtta
   101941 cgactaccga aaaaagatcg ttaagaagct atacggtgct acgtacggca atgtagtaag
   102001 tgtgctatct gtacaccacc aagcttcggt cactgtggcg gaagtagaag acgcagcggt
   102061 aaatggcgag tagcctcgtg agagcctggt aagttctcgt gagtggtgca atcccactcc
   102121 agcgacttta agcaaccgag gcatcggcgg tttaaaaata taggggtgcg caatttcgta
   102181 cgcgttttgt gcatcgtgca agttactatt acatattaga tcactctttg agtggtcttt
   102241 ttatttttgc acaaaggagg aaacaacaat gtatagacca caatacttag aacagaagta
   102301 tgaagtaatc actgttcata aaggtaatgg cgaaaaagta tatgagtata gaagaccaat
   102361 aaagagcgat acatataaac gaaaggaaaa caatgaagtt attccattgt atggcaaaag
   102421 aatagctaag cattaaataa gattgcgaaa ggagacggaa catgaccgag gaattctata
   102481 gatggctatt acagttgaca agagaagatc gtttggttaa gttctatcag tctcctaaat
   102541 ggcgcaggct tagagagaaa gcgatgaaac gagatcacta tgaatgccaa gagtgtagaa
   102601 gactaggtaa gtatcataga gtagagaacg ttcatcatat aaaggaagtc aaggatagac
   102661 ctgacttagc tttagattta gataatctta tttgtttatg tgttgaacat cataatgaag
   102721 ttcatggcag atatcttaca gcattagata aacaagagaa gaagatagaa agctttgcta
   102781 acttcgatgc aagtgaaagg tggtaagtgc atgatcatca atgataatgg cagagagtat
   102841 gatacagaaa agattgaaga gtattcatct tatactcagg gattaattaa acgtttgata
   102901 tacgttcgct atgtaggtat tagggatctg ttatcagata actgttgtag taaatacaaa
   102961 gtgaatcaag taagagaagc gttgaataaa gataataacg tcgaaagaat aaaaaatgtt
   103021 tttggatata gtattgaaga gattaattat tacattgact tcgctgaagc tttcattccg
   103081 atggtgagat aaccccccct taaaataaat cgcaaatttt ttgggggtga tgaaacggag
   103141 ggggctgtca ggaaaagaga ttttttcgaa ctttatcatg aaaggagggc taaaatgttt
   103201 aaaaacgaat tgtctcaaaa tcgctacaga gaaaaattac gccgctcttt aataagccaa
   103261 ttggaaagtc agaaaacaaa tattgagcca ttcttagata atgttgatcg ttatatcagt
   103321 ttatgggaaa cggcgatatc actggaagaa gatatatccg agaacggcat tagactggag
   103381 aatggtaaaa agaatgaatc agtagcgttg cttgtttctg tcaacaaaca aatgggattg
   103441 atgttggata aacttgccat tactcctgaa ttggtaggtg aagcaaatga atcaattcct
   103501 gagttataag catattgaaa attggttcaa agctatagaa gaaggcacta tcaaggtatg
   103561 caaagagcaa ttattgctaa aaaattatct agaagaaaga gtctttacta gagaagatat
   103621 ttacttcgat aagcagatgg tagaggattc aatcaatata ccagcacaat actttccatt
   103681 cgaattaatt ccgtgggaaa aatttctaca atgttttatt tatggtgttc gatggaaaaa
   103741 agataaaaca ctagtgttca atagatatct ttcattaatg ggacgtggta atggtaaaac
   103801 tggttttgct tcttggaaca acttctttct actaaccgct aaacacggta ttaaaaatta
   103861 tgatattgat atctatgcca ataatgaaag ccaagcaaag actagttttg atgatgtatt
   103921 taaagtaatt aaagatcatc ctgatttaga taaaaaagta tttaaagcta cgaaggaagt
   103981 tattcaaaat atcgctacaa acagcaaact tcgttataac acggcaaatg ctagaacaaa
   104041 agatgggaag cgaccaggtg caaaccgctt tgatgaaatt cacgaaaatg aagattattc
   104101 aatgataaat gtggctactt ctggtggtgg taaaattcga gattatagag aattttatga
   104161 tacaactaat ggtcatgttc gtggtggtcc gcttgatgac attatagaag aatcaaaaat
   104221 gattctttct ggagaacttg gaattgacaa ggatggagca gaattttcta gtttgtttcc
   104281 atttatttgt cgcttggata acgataatga agttgatgat cccgacatgt gggaaaaagc
   104341 ttgtccaact attaattaca atgcagattt aaaacggaaa atgtttcaag aatactctca
   104401 aatgcaacgt aatgctggtt taagacttac gttcatgacc aaacgaatga acagacctat
   104461 ggaagataca cgatttgctg ttgcttcata tgatgatgtt ctgcatacga aagaaaaaga
   104521 atttcctgaa aaaatggatg aagtgatagg aacagtcgat tttgctgata gacgagattt
   104581 tgccagcgtt gggttgctag gaaaatacga taaagatgtt tattttacac aacatacttt
   104641 tatccacgaa tcagcccttc gattacaaaa catcaaacga gaggttatag atatttctat
   104701 agatcaagga aaatcacaga tcgttcatgg aaaaaatata gaagctgatt atattgtagg
   104761 ttggtttctt gaaatgagta ataaatatta tattaaaaaa atcgctatgg atatgtaccg
   104821 tgcaaaaata ttgaagcccg ctttagaaga agcaggtttt actgtggaaa ttgttcgaag
   104881 cggatctgtt acacatggta tgttaaaaga tctggttgat gaccttttta ttaatcaacg
   104941 tttatttttt ggtgacgatg cgattatgcg ttggtattgc atgaatgtat atgaagagca
   105001 tatttctaat ggaaatatac gctatgaaaa aatagaacct gaaactagaa aaacggatgg
   105061 ctttttttca ttccttcatg gtttgaattt tttagatgat atttatgatt ctgctcctgt
   105121 aacagtcaca aatagctcag tagaaaatac aggaactgga tttactcctc tagtattcta
   105181 acttgaaagg aggtgagaaa gtggggattt ttcaaaaggc ggtaggatac ttcacaaaaa
   105241 aagcaacggt tcctttagaa gaatactttt gtaaattgca agttgatttt gtgtatcgaa
   105301 aatttgcgat tgaaacttgt attgatttga ttgcaaatgc gatgagtaaa gcggaattca
   105361 agtcatatga agatggaaaa aataaaaaga atgatcttta ctataggctg aatgtagctc
   105421 ctaataagaa aaataatgca acagaattta gaaaaaaact gatcaggaga ttaatattct
   105481 acaatgaagt attgatcgtt tctccgtcta ataattctag cgaaatattt attgcggata
   105541 gttgggatgt cacagaatat gcattgaaag atgatgtgtt ttctcaagtg caaattaaca
   105601 acatagtcct tgatagagaa tttctagaaa gtgatgttat ctatataaaa tacgcagatc
   105661 aacaaattag gcaactagtc gatgcgtatt atcaagcgta tgggaaactc atttctagtg
   105721 ccatgaatgt ttacaagcgc tctaacgctc gtagatacgt actgaaaggg aatttattcc
   105781 gaccgcaaga caatacaaca caagatcaaa tcaataaaat gatgacatca caatttaagg
   105841 cttttatgga agctgataat gcaggtgcgg tatttcaatt acaaaatgag tacacattag
   105901 aagatttcag cggaaacttc caaagcaatt caagagatat aaaaaactta atagacgaca
   105961 tctttgagat gacagcagca gcgtttcacg ttccgaaaaa cctactaaag ggagacatga
   106021 gtgggttatc ggatcaagtg gacgcttttt taatgttcga aatcataccg attgctgaac
   106081 ttattcagga tgcgtttaac gctagtctct atgaagtaga agaatacttg tcagggaatt
   106141 ttgtacgtgt ggatacaact atgatcaaga ttactagctt caaagatttg gttgacgcta
   106201 ttgatgtagg cattagaaat ggagtattta caatcaacga aggaagagaa cgcgttggaa
   106261 atgatcgctc tgataaggcg atggcagatg aaatatttat aactaaaaac aatcaacaag
   106321 tatcgaaagg aggtgaggcg aatgacgaca atgaaaacat ttctagcagt aaagaatgaa
   106381 ggcgcagtac cgcaaatttt tattcaggga tttattggtt ctagttggtt ctttgaaggg
   106441 aatactgaca agggaatcaa aaatattttg gatagtctag gtgatcaaga agaaattgaa
   106501 gtagtaatta attcaaacgg tggagacgta tttcaaggga ttgctattgg gaacttactt
   106561 aagtcaaata aagcaaaagt taacgttgtg attaacggct tagccgctag tgctgcttca
   106621 attatcgcaa tggctggcga tactataaaa atctacaaca atgcacaatt gatgattcac
   106681 cgcgcttcca catacggaga aggaaatgtc gatgacttcc gtacgattgc tgaccaactg
   106741 gaatcaattg ataaatcggt aaaggcttca tataaaacac gattcaatgg cacagatgaa
   106801 gcattgcaag aacttcttga aaaagaatcg tttatggatg cagaaacagc tttgagttat
   106861 ggattggtcg atgaaattat cgatgcagaa aatagctcag gtactgaagc taaaaaagaa
   106921 caaagcgttg aagaaatttt gaatgacgtt aaagaaaaaa gagcagaaaa aattgctgca
   106981 tttacagcag cattaaataa aacatttgga caaggagatg taaaataatg acagttaaaa
   107041 atttaaaagg tgtaacagct gcaagcgacc aattgatgaa agcttttaaa gatggtaacg
   107101 aagaatcttt tagcgcagct atggtaagct tatctaagga aattcaggat aaaattttag
   107161 aagaagcaac agcaaaaaat caagatcaat tagtattaat gaaccgtggt cagcgtgtat
   107221 taactacaca agaaacaaaa ttctataacg aagtagtgaa aaacgaaggt tttgcagggg
   107281 tcgaagaatt agtgccagct actgtatttg aacgtgtatt tgaagattta gaacaatctc
   107341 atccactatt gcaaaaaatt acttttgtta acacaactgg tgtaacagaa tggattgtgt
   107401 cacgtggagt caatccagca tggtggggta aactttgcga agctgttaaa aaagttttag
   107461 ataatggctt tgatgtaatt aacatgaagc agttcaagct atcaggttat attcctgtat
   107521 gtaaggcaat gcttgattta ggtccagtat ggttagatcg ttatgtccgt actgttttag
   107581 tagaatcatt gagaattgca ttagaacaag caattgttga tggtactggt aaagatatgc
   107641 cagtcggaat gatgcgtgac atgagcaaac aaactagcgg agaatatgct gaaaaaaaag
   107701 cagaacctat tacagcttta gatgctgtaa ctatgggcgg tttgatggcg cgactatcaa
   107761 aattcaatat cgaaggtgtg aatgatccga tttatcgtaa tgtgaatcct tctgatgtgg
   107821 tcctaattgt gaatccaact gattactggt ctaaagtatt cccagctaag actgtactaa
   107881 ctgctaatgg agaatacgta caagtattgc cagtaccagt ttcagatttg cagtcaacgg
   107941 ctgtgccaga aggaaaagca gttattgggg tagcctcaga ttacttcatg ggtgtaggat
   108001 ctacactaaa aattgaagct tcagatgaat accattttgt tgaagacgaa cgcatttatc
   108061 tagctaaaca atatgcaaac ggtcaaccta aacgtaacga tagtttcatt gtattagata
   108121 ttagcgcttt gggaactact actacaacta caaaaccaac aaccacaaca actacaacac
   108181 aagcgtaggt gatcagaatg aagtatattc tttgtcagcc ggcaatcaat cggtttaaat
   108241 gggagcttga agtttgttta actaatctga agaaactagg aatcaaagat atcgtattgc
   108301 ttttcagcag acacgatgat cagattccta ttttttttga gaaggaatat ggtgttgaag
   108361 ttcatgtgta cgatgatctg cgggacgaca aagagtatat tccttcgatt aaaccatatt
   108421 tatggtggaa atatttagaa gaagatcatt cgcgtgagga cgaccgatat ttctatatcg
   108481 attcggatgt cattttcaat aaaagaatta atttgcgcaa attgccttct aaagatgatg
   108541 tttggtattg tagcgactgc tgtagttatc taagtcttga ttatattaga agctgtgaaa
   108601 acggagaaaa tattctaaaa gatatggcaa acattgtaaa tgttacagta gaatctttgg
   108661 aaactataaa cactaattca ggaggcgcac agtgggttat taaccgtcct aaagcgaatt
   108721 attggaaaaa ggtttatctg gattctaatc ggctatatcg ctaccttaga gggcaaaaaa
   108781 caaatataca aatctggaca gccgagatgt gggcacagct ttggaacatg atgtatttca
   108841 atattggtcc taaagttcac gaggaattag acttttgttt tgctactgat ccaatagaaa
   108901 aagttaaaga agtaaaaatc ttacacaatg ctggagtaac aacaaacgat gaagatttat
   108961 ttttcaaagg gagatacgtg acttctacgc cttttgatga agatttatca tttgtaaaca
   109021 agaaaaaatg ctcttacgca tatgttaaag caattaaggc ggtggttaga tgacgcctga
   109081 acaagtgact gaagaattgc taatagctgt gaaggataat atttacgtta cctggaacga
   109141 agaagatgag tcaattaaaa agatgatagc taaaaatgct gtttatcttc aaagtaaagt
   109201 gagtacaaca ctttcttttt ctcctgaaag cttagaatac ggattgctaa tcgaaagatg
   109261 tagatacgac tggaatcgtg ctttagatga gtttgaacaa aatttcgcta gtgagttatt
   109321 aggtttcatt caacattatg cgctacaaga atatattgca ggtgatgtga atggcgaata
   109381 atcgtagact cgaagaaaca ttcaacgatg gttggttaaa gattttgacg caaactacca
   109441 aaagaaatga actaggaaaa aagattggtg tagaagatac agaaatcact tctttaaaat
   109501 ttagaaatct ttccatgaga gatagtgata taacagctat ggatgcgatg ggatcgaaat
   109561 taactaagaa agtaaagact ccatttcatc caatcgccaa gaaatttaat aaagatcaat
   109621 attttatcgt aatcgatagt atgcgttaca acgttatcta tgccgattac gataattttt
   109681 atatctattt ttatcttgaa agtgtgggtg aatatggtga ttgataattc taaagaaaaa
   109741 gaacgtttaa ataagcaaat ttctgctatc aaaacttcct tagaagagca ttttggcctc
   109801 aaactctttc aagactccgt aggcgaggat gagctacctg atgattttaa ttacttcatt
   109861 ctcgaaacag gagaaataga aatgatcact gagccaaaat atagcgtggg tcaaaatcta
   109921 tatctaactt tctattcaga aaatagagaa gatttaacag gagactcact agatattatt
   109981 tcattgattc aaaatcgttc gattcgtttt cagagaatgg atcccaatca tttaaaacta
   110041 gagaaccaag atcgctatat cgatcaattg gtatttacgt ttagacgatt attgaagagt
   110101 gattgtcatg gctaaaaata gttgggagct aaaaataaat ggacatgatg aacttcttgt
   110161 gcggatggaa cgctattcaa gcgagagcga acgactgatt aacgaagcat tgaaatcaaa
   110221 aggttcggct attgcagtgg ataggattac agaaaaaatt cctgtttctg aagcagattt
   110281 aagaagaggg caccaacacg caaaaaatag tcgtccactt aagactcaat acattaattt
   110341 gggtttcatc attagaccta caagaaaatt tgagtattta aaatatcctg atttggggat
   110401 aggtacttct aaaagaaatc agccggacga atttatgaga agaggattag gtcttgcact
   110461 tgatccaatt acagaacttc tgattcgtca attcgataaa ttaaataaat agggggaaca
   110521 acaatggcta aaacaaaaac tgtagtaaca acgttcgata acgtaagtat caaacgaatt
   110581 gcttttaatt ttaagaacgc aggaaatgca atcgcaacga attgtaacgg acaattagat
   110641 ggcgaaacag aaatgcaaac ggtggttaaa aaatgtggag cgacagaagt aaaatcaaaa
   110701 tctaaaccaa tcaatatgac ggtaacaatt actgcacatg taccgatgga agtttatcga
   110761 cgtttcaatg ggttgaaaca agatggacgt attaaaccag gcatttactc ttacggtcct
   110821 gattccgtag gcgaagattt ctcacttgct gcagagatcg tggatgactt cgaagaaaat
   110881 agaaagttag ttggtatgtt agcatgcact tcgaatacag gattaacatt ctctattgaa
   110941 aatggtgcgg atgaagtagc tgcgttagaa ctagaaacaa aagttatgca agatgaattt
   111001 ggtaaatttt atcatgaagc aattgttgca gaacttgaag aagacttaac agatcaatgg
   111061 atgacgaatc tatctgctga tgtgattaaa aaaacttcag ttgtgacaac tacggccact
   111121 caatcacagt aaaaaaaacg gaggtagcga aatgaacgaa gattactcaa aaattgaact
   111181 aaacgatgga acaattttga atttagaacc taaactgaat atcaagaaat tattgatgat
   111241 caatagagat tttaacacag acgagtttgc aaaaatgact gtgggaaaag gatccatgga
   111301 tatttctgtt attcaaggtg caaaggctgt gtatattgct taccgccaag cgaacatgac
   111361 tgattatatt tcattcgatg aatttatcga taaatgggat tttgatatgg ctactgccag
   111421 ctatatttat caattgatga tgttcaaaca agcacgcgat gcttatcaaa aagaatttga
   111481 aaaagcaaat aaggaaaaaa agcttcaaaa gtaaaaattc caaagctctt agttgaaacg
   111541 tgggtcgatg tctattcgat gttgaccgac gttttttcta tgccttcaga tttggtttta
   111601 agcgatatct gtttagatga cattttgcaa atggcttaca acaagagtgc ttatgaagga
   111661 tggaaaaact atgcaataaa ccaatcccag aaaaactaaa gaaaggaggt aaaaaatggc
   111721 taaaaagaga acagaagcag aagtaacttt catagctaac gatgacggat tgaaatctac
   111781 gttaaaagaa atcagtgctg aattaactaa aaatagagca gaattaaaac tagaacaagc
   111841 tcaattacaa cagactggtt ctgaatcaga caagttagga agtaaattat cttctttaga
   111901 aaagcagtat gaattacaaa gtcaaaaagt tgaagtaact agccaacgtt tagccaatgc
   111961 gaaaaaatat tatggagaaa attccaccga agttaagaaa cttgaaagag aactgattaa
   112021 tcaacaaaca gcgcaacaac gtttgtcaaa cgaaattgat aaaacgagta atgcactagc
   112081 tcaagcaaaa ggcgaaatac agacgtacga gtctacaatg caacagttgg atagcgaaca
   112141 aaaaaatgtt caagctagtg cttctctgat tgaatccgaa tacaaaaaat ggcaagcaac
   112201 tgctggtcaa tcagcttctg aagccgagaa attagcgaaa gcccaagaat atgtttctca
   112261 acaatctgaa aatgcggaga aaacgataga tatcctaaga cgacagttag aagctacaca
   112321 gtctgaattt ggcgctacat ccacagaagc aatgcagatg gaagcgaagc ttaatgatgc
   112381 tgaacgtgaa tttgaagagt taggacaagc tgctaaaaat gtagatacaa ctaacttgga
   112441 cgatatcgga agcaaaatag atatgaataa tttaatggaa gcttctgacg ttttaagcga
   112501 cattggcgat aagcttacag aattagggaa acaagcagtg gactctgcta acagtgtagg
   112561 tagttcccag agtaagatac aagctaattt tggtttgtct aaacaagagg ctgaagaatt
   112621 aacgaatgta gccagagaca tttattataa aggttttgga gaatcgttag atcagtccac
   112681 agatgcattg attttggtaa agcgtaattt aggagattta aataatcaag atttacaaaa
   112741 tatcacggaa caagctatgg tcctagaaaa caccatgggc gctgatatgg atgaaacgtt
   112801 acgtggtgta aatggcttaa tggtcaattt cggcttgagt gctcaagatg caatggattt
   112861 aatggtttcg ggtactcaaa acggtttaga taaaacgcac gaattaggcg acaatatggc
   112921 agaatatagc caattatgga gtcaaatggg atattcagct gatgaaacgt tcggaatgct
   112981 tcaaaatggt ttagatgcgg gtgcttataa ccttgataaa gtcaatgact tagttaagga
   113041 aatgggaata tcgttaacag atggtcgatt tgagcaaaac atggatatgt ttagtgaaag
   113101 tactagaaaa gcttttgaag agtggaaaaa tggcggagga acacaaaaag acgttattaa
   113161 ttccatgatt caagatttta gcaatatgga tggtcaatac gaccaattaa ataaagcttc
   113221 gacaatttgg tctgcgcttg gcgaagataa tgcgatgaaa gttgtccaat ctttaactga
   113281 tgttaaccat acatttgatg atgttagtgg atctgcacaa aaaatgaatg aagattctac
   113341 tactccgttg caagagttga acgggaaaat agctgaatta aaggattcat tagctcctat
   113401 aggcaacaca atcatagatg cactcgaacc agtaattgat tttctaggaa agatggctga
   113461 tgcgtttaat aatcttccac aaccagtaca ggatttcatt gttgctatag gtggtcttac
   113521 agcagcattt ggcatactgt taccggcaat actagccgtg tcatatttat ttggtccgat
   113581 gatgcttata attggaggaa ttatagctgt tatagcaggc gttattgtgg caattaagaa
   113641 ctggggtgca attactgact ggtttagtgg tttatggaaa aaatttactg attggttggg
   113701 tgatacttgg gaaagtataa aagacggagc ctcatcagtt tgggatgggg ttaaagaaac
   113761 ctggtctgga tttgtagatt gggttcaaga tatttggcaa ggagtttctg attggtttgg
   113821 agagctatgg agcggattag ttgaaggagc ttccaacatc tggcaaggag tccaagaaac
   113881 ttggcaaaca ttcgttgatt gggtttcaaa tatttggaac ggagtcaaag aagtatggtc
   113941 gattatttgg gcagacattg taggaattgt tcaaatacca tggacattaa taacgtcatt
   114001 gattcaagct ggtattaata ttatcgtggg tatttttgat gtagctggac agtgattggg
   114061 cgcagcttgg caagctgttt ggacacctat ttctgatttc cttaaaaata cttgggatac
   114121 tatgacacaa tggataagta tcgcttggaa tgggattgta actacattcc atactatatt
   114181 tgatccagta gtggcatggt ggaatggtat atggacagct attagtacta cggcttcaaa
   114241 tatttggaat tcaattagtg caacagcttc tagtatttgg aacagtatca agaatacaat
   114301 cactagcttg gtacaagcag ctgctacagt aattcaaaat atttggtcaa ctgtatctag
   114361 ttggttaggt ggaatttgga attcaatcag ctctacagca tcaaatatct ggaatagtgt
   114421 gactagtagt ataagcaatg ctataaacgc agctaaaagt gccattcaaa gtgtttggaa
   114481 tagtatatct tcgtggatta gcggaatttg gaacggtatc aaaaatactg ctttgaatct
   114541 ttggaatgga attacaagca ctattagctc taaagtaaac gatggaaaaa atgcaatttc
   114601 aagcggttgg tccaatctaa caggtattgt ttccgacata ttcaataatg ttaaaagtac
   114661 aattgctaac atatgggaag gcatcaaaaa gactgttagc gctccaattg attggattag
   114721 agacaaaatc agtggcattt ttgataattt gaatatttct ataccacata ttccgttacc
   114781 ggaatttatc atggaaggca gctttaaccc gctaaaaggt caaatccccc atttgcgtgt
   114841 aaaatggcat gctaaaggag gtatctttac taaaccaact ttactaggtg gaatgaacgg
   114901 tgtcggtgaa gcaggacctg aagcagtttt acctttgaaa agatctgttt tgcaagaaat
   114961 tggtgatcgt atcttgagta gcacatcagt ttcatctagg gcacaaacga ttcaacctgt
   115021 gaataactac gaattcaatt tcacaattga tggtaacgca gatgaggtta ctatgaagca
   115081 aacaactcaa caaatcattg atagcattac aaaaattcaa aatgataatg cttcggcatg
   115141 gcgttaaaca ggagagtatt tctcctgttt ttttagtatt aaaaaggatg tgaaaaaatg
   115201 actgattgta tacattctat aatcgatgga tttcctgatt atttgcataa attggcttta
   115261 gctgaaagac caaccatacc ttctccaaaa agacagagag ttgaaacttc tgttttaggt
   115321 aggttaggtg gcttagtaca agattactcg tttgaagata tgtcgtttac attgcactat
   115381 aactatttag aggatgtgga agaccatcaa gcgttcaagc aatcgtttta tatcatgcgt
   115441 cattggttaa actatgcaaa gaaattagaa ttctctgacg atcccaacgt ctattacgtt
   115501 atccagacta tcgatattgg ggatgcagaa aacgatattg ttgaatgggg agagttcgat
   115561 gtaaatatta ctgcgaaacc attcgcaaga gttcaagaag atgtacctat aaccgtagat
   115621 aaaccacagt catttaactt gctgaataat agtttagaag aaagttttcc aaagattatc
   115681 atcactcctt cagctacttc atgccagttc atcttaaatg attatgtgtt tagtttcgaa
   115741 ggcttagtag caggaactga tgtagtcatt gatagtgatt tgatgctttg ctacgaagag
   115801 caatcggacg gagatatttt agatcggtcc aacaaaatga agaccatgca atatccgaca
   115861 ttgcaagtgg atatcaatca ttttaattgc actggtttga gcaaaataca aatttatcgt
   115921 aacgggttaa ggtaggtgaa atagatgatc gataatttaa taactattta cgataaaaat
   115981 gacgcgaata atttagctga acatttatat gatacgcaag gtttaggcgc tttgtcagac
   116041 tggttaacag ctactgttag caataaacta aacggagccg agatatttca gggtacttat
   116101 tcaataaacg gaactaatgc agatttgatt gtagacggac gtattattca gtgttatgta
   116161 gatgaaaatc gagcaaaaca gcgtctacgg atttattatg caaaaacttc tgtaataggc
   116221 aatacgatag aagtaaaagc tgaacctatt ttcaatgata taagaaaatc ggtgttgaat
   116281 aaatatgaca gcggaacaga aaagatcact gctactcagg catggcaaaa cgcaaaagtt
   116341 ttagcgaaac cagctatccc ttcgcagttt tctttctcgt cattagtaga tacgcttgct
   116401 aatgtgaaga tagaaaaggc gaatttttta gaattctttg gtggaaaaga gggatctatt
   116461 ctagatcgat ttcatggtga gtttctaaaa gataataaca cattacgtca tgaaaaaagt
   116521 ctaggtacgg atcataaaat caaagcgatt tatactaaaa acttaactgg tcttgactta
   116581 gagattgatg ctcaaagtgt tttagttgga gtttatccat tcattagcag ctcttcagaa
   116641 ggagaagacg agatcactct accagaagaa gttattttca cggattacgt ggatgattat
   116701 cctgctggat atgtttcttt tgttgatttt aaagacaaag cgactgatgt agccacatta
   116761 agggaagctg ctaaagactg gttgaaaaca aacatagata aacaaaaacc acaagtgagt
   116821 ggttcgattg aattagtacc attgaggcat caaagaggct atgaaaaatt tgttgatcta
   116881 gaaaaagttt cgatgggtga cggagtagat gtgtatcatc cacagttaaa agtgaatatg
   116941 tcagcgagaa ttgtggaata tacgtttaat gttctaacca attcatacga taaattagtt
   117001 gtaggaaacg tcaaaacaaa cttcttagaa aacacagaga ataatgtcag caatttgatt
   117061 aatgatgcca ttgatcaatt gaaaaacggt ggcgaaatca gtgatttact caatgatatt
   117121 gtagatcatc aaactgatat gattactggt caaaatggtg gttatgtttt attagatcct
   117181 aaagaagcgc ctagtcgtat tttgattatg gacacaccag ataagaatac cgcaaggaat
   117241 gttttacaaa tcaacaatgc tggtattggt ttttctaaaa ctggcattaa tggaacatat
   117301 gaaacggcat ggacgttaga tggcggattc aatgcctcgt ttattacggc tggtgaaata
   117361 gtaggaatta ctattagagg tactacatta attagtgatg gtgctgatta tagaacaagt
   117421 attgctaatg gcaaaatgac ttggtactca aaaaaagtta acaaagatat tatggagcta
   117481 gaagcacgtg attatgtaag tgctgatgcc ggtattgtat catacaccat gaaaactggt
   117541 ggtggtttca tgattagaaa tccacagggt aacttggttt ttagtacgtg ggataatggt
   117601 aataacagac cgtttctatc ttttggtgcg cccaatttca ggtatagcaa tgctagttat
   117661 gtaacttctg gcgacggtag ttctttaagc attaatggta gtgcgggtaa ctcatgggaa
   117721 tttaaggtag ctggtaggac tatgaaattt actagtgatg gtatgctaac gttaccaggt
   117781 tgtttttttg gttcatggga agatgggaaa cttgctaggt ttgaacaatc aacggtacaa
   117841 gtatataaag attttactgt tagaggtact aaaaactcaa ctgtaccaac agaacattat
   117901 ggacaacgac tattgaacgc ttatgaaact ccagaatatt atttcgctga ttatggggaa
   117961 gccgttacag gtgacaatgg taaagttcgt gttgatattg accccatgtt tgctgaaaca
   118021 gtaaatctaa gtcggtatat gacacatgtg acacctacag aactagtttt gtgtgctgtt
   118081 actcatgaag atattgacca tttcatcatt gaaactagta agccaaacgt attagttaga
   118141 tggaatttag tggcacaccg tctagggtat gaagatatta gattaaaaga ggatacagca
   118201 tatgatagca cagtgcttga ccaaaaacgt ttttaaaacg aagacaagga ggtatataaa
   118261 tggctagcag tttatataat ttggctttag atttcagcaa agaattaaac tacaccaaag
   118321 ctattatggc tcgtcaaggt gataaaggga ttacggtgac ggttaaaccg tttctaaatg
   118381 gcttgcagat ggatacgagt ggcggaacat ttactttaaa aggaacaaca ccatctaacc
   118441 gttacgtaga taatgttgca actagtgtaa ctagtgaaga agtcacgttt tctcttgatg
   118501 gcacatttat gagtgaagca ggatattata aacactgcta cgtagaatat agaaaagaca
   118561 atcaaatttt aacaacgcaa gatatcattt ttttctcact aggagtgtct gacatttcgc
   118621 aaggccaagc cgatgaatat gtttcgcaat tagaagagtt gattcgaaag tacaacgaaa
   118681 cttttgatgc ttttatggct gaaatcaaag gtagagtgga tagcttaaat caacagatta
   118741 ctgatttaac tggtcaagct aaaacgctac aagacaagtt agatgctctg aaagaagaaa
   118801 tttctaagtt aggtaactta caagtgatgt acagtaacag catcgacttc ggggactatg
   118861 attattcgga gaacccgaat ttaatgccat atattacaga accttgggtt ggacctttgt
   118921 taggtaatgg acatacagtt aaagatagtg ttaaaagggt tataactcat actaaaacta
   118981 gaacagctaa ttccggcgac atattatcgc ttggcttagg tattccgtgt acagcggaag
   119041 ctaataatag gtatcttata accactttac gaccatctac gacatacaca ttaagcgtta
   119101 ctatgagtgt cggttcagat tggactggag agaccaatac tataggggtt aggcttagat
   119161 atttaaatga acaaggtggt atagaactgc ccattaatgc attaatacca gcaaatgtag
   119221 aacgcgataa aatggtcact catacattta ctggtataac aaaagataat gtaactagca
   119281 taacaaactg ttatgttgaa atattctcac ttaatagtga atataaagga acggttagtg
   119341 tcagctatga cgttaagtta aaagcacact accctaactt actagatggc ccttactggt
   119401 taggtaaagt tcctttaggc gagaatattg ctgaccctac agttgtcttt ccacacaaaa
   119461 ctagtgaata tatggtttat ggtagacgca atacagaaaa ctatatcgct gaccaaacat
   119521 ataccatatc aatgaaagca actaaactaa cagttcaatc gtttgccgtg tatattgctg
   119581 ctggccgcgt taaagtagga gacatgaagc ctactgaggg gttagctaat acatgggagt
   119641 taacttttac tgtaactaag caacatatag atagtggggt aactaattat ctggaaatat
   119701 accaataccc gtcagctaca aaaggagctg ttcaaattga gtggctcaaa ctagaaaaag
   119761 gcaatactcg aaccccgaat attagtgagt ataaatatcg tggtactggt atgcgtgatt
   119821 caaacaatcc aaaagattat gtttgggatc tagcaccaga atatgtcgaa gataatttgg
   119881 ccacagatat taaaatttct gaaattactg gtaaagcaaa caattatacc gatgggaaag
   119941 tatcggagat taattcgcag ttgactgctt caattaatga agtagacacc acagctaagg
   120001 atgctcaaac aaaagcgaat gctaatgcga ctgctataga tgaattagac aataagatcg
   120061 atgaacgcat taatgataca gctactacca cattaacagt tacaaacggg aataccggat
   120121 cagcaaagct ttatcgtgaa ggaaaaacag tttctatata ttttgtggct ttaaacggaa
   120181 aaagcagtgg tggaaatgat tcaacgatac taacaattcc agaaggctat cggccaccaa
   120241 ttagttttga gcaactggtt ggctcgatag accgttctac tttgaacagt gctcagttat
   120301 ctattggtgc agatggagcc attaaatggc gaagaaactc aagttatgga tcggattata
   120361 cctttgcaat tacttacacg atttagaaaa gcgtgaatcg atatgaaggc agcatataga
   120421 ccaattgaac cttacggatt cgagcaaatc attgtgaatg atgaagaaca tttaccggaa
   120481 gaatgcacag aagtcgaacc accgattcca aattggaaac cgaaattcaa ttactgggag
   120541 ggaaataaat gaaaaacatt tggaaatatg gacgtactgg cggagagtac gcaggaaaag
   120601 tattggacga catgcttgta tccgttcctt acacagatca gccaccgctt gaaggaattc
   120661 gtgctgatgg cgaaccgtta acaatcgctg atcaaatgtt tgatcctaaa ttgaatcaat
   120721 ggattatttt agcgaacgca ctagatcaca acgatttaaa caatctcaaa gcaatgtatg
   120781 agtcgttaga aaatgagaac ggcgatttaa aacagatcaa tgccaaactc atgctaagcg
   120841 atgtagcaat taaacaggaa aatactgcat tgaaagaaaa agcggatagt ttagcacaaa
   120901 tcaattcaaa aatgatgctt gcttcgttac aaaatagcaa agacatttca gaaattaaag
   120961 agcaactaaa tccagcttca aagggaggtg agtagtatgt ttagttttag cgatgtgaaa
   121021 atgatgtatg attggggctg tttcactaac gaacaagtaa tggttttcgt tccgttgtgc
   121081 attactgaag aaaaagcaga taaaatcatt agcaaagaag agagcgcatc ttaattgatg
   121141 tgctttttat tttgattcaa ggagttgtca catgattaat ttaggggaat ggggagcgat
   121201 agcaggatca ataaccgcta tcgtttcttt gattttatta gtaataaaac caattactgc
   121261 atctttctcg aagattactg agactctttc aaaagtaagt cacaatttag atttgctgac
   121321 taaagattta gaatcgagca aatcagatcg attgatgatt catgaagaac taaagaaaca
   121381 cgatgaaaga ttagatacac atgcagaaaa attggtagaa cacacgcaac aaattaaaac
   121441 tttatttagg gagagaagaa aatgaataat aaaacgttcg aagtactaaa atggttcgca
   121501 ctggtaatta ttcccgcact agctactttc gtggggttag ttggtaaagc gctcaattgg
   121561 cagtacacag atatctgtgt tgtcatcatt actggttttg gcgcgttttt agggagtgtg
   121621 ttgggtgtat caaatcgaac ctacaaaatg ttctcggctg aaagcgaaga aggaggaaac
   121681 aaatgaaaaa gaaaattact attactgcga tgagcctgtt aacggctctt tttttattac
   121741 ccattaatac gtttgcttat actattaatg acgagtataa tttagcgccg aatcaaggag
   121801 actccagatt agcaattcct aacaaaatta ttttgcacga aactggaata gatgcaccag
   121861 caagaaacgt agccgccaac atgaaaaata attataacgg aagcaatcct tatactacag
   121921 atgttattgg tgacggtggg attgtttacc gtgtgggtga gcaaggatat gtttcgtggg
   121981 gagctggtaa cgctaatcct tatgcgcctg tacagattga attacagcgc acatatgata
   122041 aagcattgtt tgaaaaaaac tatcgagctt atattgaata tacaagagat agtgcaaaaa
   122101 aatatggaat tccattgact cttgatcaag gaacttcttt atttacaaaa ggaatcattt
   122161 ctcatttgtg ggtgacaaat tatgtttggg ggaaccacac agatccatat ggttacttat
   122221 cgcaaatggg agttagcaaa gaaaagcttg cttatgattt agctcatgga tttaccgatg
   122281 aaaatccaac aacttctgaa aacaagcctg tcattgatcc aacacgagct ggtgcagcta
   122341 atcctacgct gacagatgga acaaatcacg cccacattga tcagtttggg gaaatcgaaa
   122401 acgcaaactt gcatgtggct ggatggcaca ttgctaacta taaatacgag tatattttca
   122461 ttatggacta caatactggg aaagaattag ctcgagtaag agctgatgga atttatagac
   122521 cagatgtaaa ccaagcttat aatacttctg gaaatgttgg ttatcatgta tctttcaata
   122581 tgcgtaattt tcctagtaag aaagtctatg taatgatgcg ggcaacgaat gatccagagg
   122641 gaaacactaa aggcggtgcg caagatttcc atgacaaacg ttggtattta aatattccta
   122701 aacgataaaa atagctcctc gttgaggagc agtacataac tatattgaca actataaaaa
   122761 ttattcgata aaatagtgat gttatcgcat atcttcacta tcacccataa atagtcacac
   122821 tccaagctat gcgataacag gtttgttgcc acacattcta ctggttgatt gtttatggct
   122881 ttatgtggca acaaccagta cccttagctc agttggtcag agcagacggc tcataaccgt
   122941 ccggtcgtag gttcgagtcc tacagggtac attaacgtag ccatttgaat cgttctgtgt
   123001 tagaattttt ttgaagagta ttatacaagc taaagctttt cttcattgcc actcaaatga
   123061 gtggcttttt tatgtatcct tttatggatt aatgaaagga tgtttcacat agttatactt
   123121 ctgtatattt gaaaagtttt actttgattt ttaaatagaa agacatttgg gttaaattgt
   123181 gagataataa taaagaagag tttaaagcgc accccaaacc acttccccat aagtgtgtta
   123241 cgctttaaac tcttttatat ttgaagccat taaaaagcat accatatttt tgaaaaaaag
   123301 tgagaaaaaa ggcttacaat tggagtggta gttaattagt gacttatttt tgattttata
   123361 gcactgatac tataaaatat agatatcatc atattacaca atcttaatac taacttaaaa
   123421 aatatctcct ttcacaagta tggtgataaa attcgttccg ggctaccttt ttaggtagcc
   123481 tactttaatc tttatacctt tctggatcaa cgaaagtata ctttatatag tcataacgcc
   123541 gatgatcgct acgtgcgtcc ggcacgtcag tc
//
LOCUS       DACGLW010000002       104696 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.002, whole genome shotgun sequence.
ACCESSION   DACGLW010000002 DACGLW010000000
VERSION     DACGLW010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 104696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 104696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..104696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.002"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            <1..282
                     /locus_tag="IXT53_000145"
     CDS             <1..282
                     /locus_tag="IXT53_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HAQ4904008.1"
                     /translation="FEEARQAYEKQWNEIHQNYEYINAKSNFLSAFWNDGDERWCEEC
                     DDDLQQYKGLALLKNHQPITVESRKEFYETTNSSGKTKRCERPKQGAWC"
     gene            215..2287
                     /locus_tag="IXT53_000146"
     CDS             215..2287
                     /locus_tag="IXT53_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ4904009.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(2498..3445)
                     /locus_tag="IXT53_000147"
     CDS             complement(2498..3445)
                     /locus_tag="IXT53_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ4904010.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            3691..4155
                     /locus_tag="IXT53_000148"
     CDS             3691..4155
                     /locus_tag="IXT53_000148"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HAQ4904011.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(4217..5260)
                     /locus_tag="IXT53_000149"
     CDS             complement(4217..5260)
                     /locus_tag="IXT53_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HAQ4904012.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            5611..6474
                     /locus_tag="IXT53_000150"
     CDS             5611..6474
                     /locus_tag="IXT53_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ4904013.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(6603..8225)
                     /locus_tag="IXT53_000151"
     CDS             complement(6603..8225)
                     /locus_tag="IXT53_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ4904014.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            8474..8995
                     /locus_tag="IXT53_000152"
     CDS             8474..8995
                     /locus_tag="IXT53_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4904015.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(9181..10911)
                     /gene="spxB"
                     /locus_tag="IXT53_000153"
     CDS             complement(9181..10911)
                     /gene="spxB"
                     /locus_tag="IXT53_000153"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HAQ4904016.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            11076..11894
                     /locus_tag="IXT53_000154"
     CDS             11076..11894
                     /locus_tag="IXT53_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HAQ4904017.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKHHFAVFGVMSGFIIMMILDVALG"
     gene            complement(11997..12194)
                     /locus_tag="IXT53_000155"
     CDS             complement(11997..12194)
                     /locus_tag="IXT53_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4904018.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            12363..12941
                     /locus_tag="IXT53_000156"
     CDS             12363..12941
                     /locus_tag="IXT53_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAQ4904019.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            complement(13986..14489)
                     /locus_tag="IXT53_000157"
     CDS             complement(13986..14489)
                     /locus_tag="IXT53_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAQ4904020.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            14848..15873
                     /locus_tag="IXT53_000158"
     CDS             14848..15873
                     /locus_tag="IXT53_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ4904021.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            16196..17074
                     /locus_tag="IXT53_000159"
     CDS             16196..17074
                     /locus_tag="IXT53_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4904022.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            17550..17666
                     /locus_tag="IXT53_000160"
     CDS             17550..17666
                     /locus_tag="IXT53_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAQ4904023.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            18594..18932
                     /locus_tag="IXT53_000161"
     CDS             18594..18932
                     /locus_tag="IXT53_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4904024.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            19026..19256
                     /locus_tag="IXT53_000162"
     CDS             19026..19256
                     /locus_tag="IXT53_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4904025.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            19656..20027
                     /locus_tag="IXT53_000163"
     CDS             19656..20027
                     /locus_tag="IXT53_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HAQ4904026.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(20301..20993)
                     /locus_tag="IXT53_000164"
     CDS             complement(20301..20993)
                     /locus_tag="IXT53_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ4904027.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(20996..21781)
                     /locus_tag="IXT53_000165"
     CDS             complement(20996..21781)
                     /locus_tag="IXT53_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ4904028.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            22018..23418
                     /gene="lacG"
                     /locus_tag="IXT53_000166"
     CDS             22018..23418
                     /gene="lacG"
                     /locus_tag="IXT53_000166"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAQ4904029.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            23443..23760
                     /locus_tag="IXT53_000167"
     CDS             23443..23760
                     /locus_tag="IXT53_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ4904030.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            23773..25446
                     /locus_tag="IXT53_000168"
     CDS             23773..25446
                     /locus_tag="IXT53_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ4904031.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            25460..27091
                     /locus_tag="IXT53_000169"
     CDS             25460..27091
                     /locus_tag="IXT53_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinogalactan endo-1,4-beta-galactosidase"
                     /protein_id="HAQ4904032.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(27582..29147)
                     /gene="guaA"
                     /locus_tag="IXT53_000170"
     CDS             complement(27582..29147)
                     /gene="guaA"
                     /locus_tag="IXT53_000170"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HAQ4904033.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(29206..30129)
                     /locus_tag="IXT53_000171"
     CDS             complement(29206..30129)
                     /locus_tag="IXT53_000171"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HAQ4904034.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            30325..30930
                     /locus_tag="IXT53_000172"
     CDS             30325..30930
                     /locus_tag="IXT53_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ4904035.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            31238..32254
                     /locus_tag="IXT53_000173"
     CDS             31238..32254
                     /locus_tag="IXT53_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ4904036.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            32395..33696
                     /locus_tag="IXT53_000174"
     CDS             32395..33696
                     /locus_tag="IXT53_000174"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HAQ4904037.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            33742..34404
                     /gene="deoC"
                     /locus_tag="IXT53_000175"
     CDS             33742..34404
                     /gene="deoC"
                     /locus_tag="IXT53_000175"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HAQ4904038.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            34425..34823
                     /locus_tag="IXT53_000176"
     CDS             34425..34823
                     /locus_tag="IXT53_000176"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HAQ4904039.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            34889..35971
                     /locus_tag="IXT53_000177"
     CDS             34889..35971
                     /locus_tag="IXT53_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ4904040.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            36183..37745
                     /locus_tag="IXT53_000178"
     CDS             36183..37745
                     /locus_tag="IXT53_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4904041.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            37738..38871
                     /locus_tag="IXT53_000179"
     CDS             37738..38871
                     /locus_tag="IXT53_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ4904042.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            38871..39827
                     /locus_tag="IXT53_000180"
     CDS             38871..39827
                     /locus_tag="IXT53_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ4904043.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            39926..41092
                     /gene="deoB"
                     /locus_tag="IXT53_000181"
     CDS             39926..41092
                     /gene="deoB"
                     /locus_tag="IXT53_000181"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HAQ4904044.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            41116..41934
                     /locus_tag="IXT53_000182"
     CDS             41116..41934
                     /locus_tag="IXT53_000182"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAQ4904045.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            41951..42658
                     /gene="deoD"
                     /locus_tag="IXT53_000183"
     CDS             41951..42658
                     /gene="deoD"
                     /locus_tag="IXT53_000183"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HAQ4904046.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(42956..43642)
                     /gene="gpmA"
                     /locus_tag="IXT53_000184"
     CDS             complement(42956..43642)
                     /gene="gpmA"
                     /locus_tag="IXT53_000184"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAQ4904047.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(43814..44395)
                     /locus_tag="IXT53_000185"
     CDS             complement(43814..44395)
                     /locus_tag="IXT53_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ4904048.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(44528..45211)
                     /gene="rpiA"
                     /locus_tag="IXT53_000186"
     CDS             complement(44528..45211)
                     /gene="rpiA"
                     /locus_tag="IXT53_000186"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HAQ4904049.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            45530..45943
                     /gene="rpsL"
                     /locus_tag="IXT53_000187"
     CDS             45530..45943
                     /gene="rpsL"
                     /locus_tag="IXT53_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HAQ4904050.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            46027..46497
                     /gene="rpsG"
                     /locus_tag="IXT53_000188"
     CDS             46027..46497
                     /gene="rpsG"
                     /locus_tag="IXT53_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HAQ4904051.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            46591..48678
                     /gene="fusA"
                     /locus_tag="IXT53_000189"
     CDS             46591..48678
                     /gene="fusA"
                     /locus_tag="IXT53_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HAQ4904052.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            48850..50037
                     /gene="tuf"
                     /locus_tag="IXT53_000190"
     CDS             48850..50037
                     /gene="tuf"
                     /locus_tag="IXT53_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAQ4904053.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            50444..50752
                     /gene="rpsJ"
                     /locus_tag="IXT53_000191"
     CDS             50444..50752
                     /gene="rpsJ"
                     /locus_tag="IXT53_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HAQ4904054.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            50783..51412
                     /gene="rplC"
                     /locus_tag="IXT53_000192"
     CDS             50783..51412
                     /gene="rplC"
                     /locus_tag="IXT53_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HAQ4904055.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            51440..52063
                     /gene="rplD"
                     /locus_tag="IXT53_000193"
     CDS             51440..52063
                     /gene="rplD"
                     /locus_tag="IXT53_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HAQ4904056.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            52063..52353
                     /gene="rplW"
                     /locus_tag="IXT53_000194"
     CDS             52063..52353
                     /gene="rplW"
                     /locus_tag="IXT53_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HAQ4904057.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            52392..53225
                     /gene="rplB"
                     /locus_tag="IXT53_000195"
     CDS             52392..53225
                     /gene="rplB"
                     /locus_tag="IXT53_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010747214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HAQ4904058.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            53268..53546
                     /gene="rpsS"
                     /locus_tag="IXT53_000196"
     CDS             53268..53546
                     /gene="rpsS"
                     /locus_tag="IXT53_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HAQ4904059.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            53569..53916
                     /gene="rplV"
                     /locus_tag="IXT53_000197"
     CDS             53569..53916
                     /gene="rplV"
                     /locus_tag="IXT53_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HAQ4904060.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            53930..54586
                     /gene="rpsC"
                     /locus_tag="IXT53_000198"
     CDS             53930..54586
                     /gene="rpsC"
                     /locus_tag="IXT53_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HAQ4904061.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            54589..55023
                     /gene="rplP"
                     /locus_tag="IXT53_000199"
     CDS             54589..55023
                     /gene="rplP"
                     /locus_tag="IXT53_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HAQ4904062.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            55013..55201
                     /gene="rpmC"
                     /locus_tag="IXT53_000200"
     CDS             55013..55201
                     /gene="rpmC"
                     /locus_tag="IXT53_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HAQ4904063.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            55226..55492
                     /gene="rpsQ"
                     /locus_tag="IXT53_000201"
     CDS             55226..55492
                     /gene="rpsQ"
                     /locus_tag="IXT53_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HAQ4904064.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            55550..55918
                     /gene="rplN"
                     /locus_tag="IXT53_000202"
     CDS             55550..55918
                     /gene="rplN"
                     /locus_tag="IXT53_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HAQ4904065.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            55955..56263
                     /gene="rplX"
                     /locus_tag="IXT53_000203"
     CDS             55955..56263
                     /gene="rplX"
                     /locus_tag="IXT53_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HAQ4904066.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            56291..56830
                     /gene="rplE"
                     /locus_tag="IXT53_000204"
     CDS             56291..56830
                     /gene="rplE"
                     /locus_tag="IXT53_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HAQ4904067.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            56849..57034
                     /locus_tag="IXT53_000205"
     CDS             56849..57034
                     /locus_tag="IXT53_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HAQ4904068.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            57070..57468
                     /gene="rpsH"
                     /locus_tag="IXT53_000206"
     CDS             57070..57468
                     /gene="rpsH"
                     /locus_tag="IXT53_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HAQ4904069.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            57500..58036
                     /gene="rplF"
                     /locus_tag="IXT53_000207"
     CDS             57500..58036
                     /gene="rplF"
                     /locus_tag="IXT53_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HAQ4904070.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            58197..58553
                     /gene="rplR"
                     /locus_tag="IXT53_000208"
     CDS             58197..58553
                     /gene="rplR"
                     /locus_tag="IXT53_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HAQ4904071.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            58574..59074
                     /gene="rpsE"
                     /locus_tag="IXT53_000209"
     CDS             58574..59074
                     /gene="rpsE"
                     /locus_tag="IXT53_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HAQ4904072.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            59089..59268
                     /gene="rpmD"
                     /locus_tag="IXT53_000210"
     CDS             59089..59268
                     /gene="rpmD"
                     /locus_tag="IXT53_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HAQ4904073.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            59312..59752
                     /gene="rplO"
                     /locus_tag="IXT53_000211"
     CDS             59312..59752
                     /gene="rplO"
                     /locus_tag="IXT53_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HAQ4904074.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            59752..61047
                     /gene="secY"
                     /locus_tag="IXT53_000212"
     CDS             59752..61047
                     /gene="secY"
                     /locus_tag="IXT53_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HAQ4904075.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            61109..61756
                     /locus_tag="IXT53_000213"
     CDS             61109..61756
                     /locus_tag="IXT53_000213"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HAQ4904076.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            61947..62165
                     /gene="infA"
                     /locus_tag="IXT53_000214"
     CDS             61947..62165
                     /gene="infA"
                     /locus_tag="IXT53_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HAQ4904077.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            62198..62314
                     /gene="rpmJ"
                     /locus_tag="IXT53_000215"
     CDS             62198..62314
                     /gene="rpmJ"
                     /locus_tag="IXT53_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HAQ4904078.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            62332..62697
                     /gene="rpsM"
                     /locus_tag="IXT53_000216"
     CDS             62332..62697
                     /gene="rpsM"
                     /locus_tag="IXT53_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HAQ4904079.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            62725..63114
                     /gene="rpsK"
                     /locus_tag="IXT53_000217"
     CDS             62725..63114
                     /gene="rpsK"
                     /locus_tag="IXT53_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HAQ4904080.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            63194..64132
                     /locus_tag="IXT53_000218"
     CDS             63194..64132
                     /locus_tag="IXT53_000218"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HAQ4904081.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            64160..64540
                     /gene="rplQ"
                     /locus_tag="IXT53_000219"
     CDS             64160..64540
                     /gene="rplQ"
                     /locus_tag="IXT53_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HAQ4904082.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            64812..65651
                     /locus_tag="IXT53_000220"
     CDS             64812..65651
                     /locus_tag="IXT53_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ4904083.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            65627..66496
                     /locus_tag="IXT53_000221"
     CDS             65627..66496
                     /locus_tag="IXT53_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ4904084.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            66486..67283
                     /locus_tag="IXT53_000222"
     CDS             66486..67283
                     /locus_tag="IXT53_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAQ4904085.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            67486..68232
                     /gene="truA"
                     /locus_tag="IXT53_000223"
     CDS             67486..68232
                     /gene="truA"
                     /locus_tag="IXT53_000223"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAQ4904086.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(68565..71234)
                     /locus_tag="IXT53_000224"
     CDS             complement(68565..71234)
                     /locus_tag="IXT53_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HAQ4904087.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
     gene            complement(71472..71660)
                     /locus_tag="IXT53_000225"
     CDS             complement(71472..71660)
                     /locus_tag="IXT53_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HAQ4904088.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            71930..72292
                     /locus_tag="IXT53_000226"
     CDS             71930..72292
                     /locus_tag="IXT53_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ4904089.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            72344..74026
                     /locus_tag="IXT53_000227"
     CDS             72344..74026
                     /locus_tag="IXT53_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HAQ4904090.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            74143..76179
                     /gene="recG"
                     /locus_tag="IXT53_000228"
     CDS             74143..76179
                     /gene="recG"
                     /locus_tag="IXT53_000228"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HAQ4904091.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDERPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            76228..77229
                     /gene="plsX"
                     /locus_tag="IXT53_000229"
     CDS             76228..77229
                     /gene="plsX"
                     /locus_tag="IXT53_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HAQ4904092.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            77351..77593
                     /gene="acpP"
                     /locus_tag="IXT53_000230"
     CDS             77351..77593
                     /gene="acpP"
                     /locus_tag="IXT53_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAQ4904093.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            77861..78865
                     /locus_tag="IXT53_000231"
     CDS             77861..78865
                     /locus_tag="IXT53_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4904094.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            78866..79810
                     /locus_tag="IXT53_000232"
     CDS             78866..79810
                     /locus_tag="IXT53_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4904095.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            79810..80772
                     /locus_tag="IXT53_000233"
     CDS             79810..80772
                     /locus_tag="IXT53_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ4904096.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            80799..81713
                     /locus_tag="IXT53_000234"
     CDS             80799..81713
                     /locus_tag="IXT53_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ4904097.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            81739..83520
                     /locus_tag="IXT53_000235"
     CDS             81739..83520
                     /locus_tag="IXT53_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ4904098.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            83868..84554
                     /locus_tag="IXT53_000236"
     CDS             83868..84554
                     /locus_tag="IXT53_000236"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAQ4904099.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            84574..88155
                     /gene="smc"
                     /locus_tag="IXT53_000237"
     CDS             84574..88155
                     /gene="smc"
                     /locus_tag="IXT53_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HAQ4904100.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            88164..88973
                     /locus_tag="IXT53_000238"
     CDS             88164..88973
                     /locus_tag="IXT53_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ4904101.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            88984..89982
                     /gene="ftsY"
                     /locus_tag="IXT53_000239"
     CDS             88984..89982
                     /gene="ftsY"
                     /locus_tag="IXT53_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HAQ4904102.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            90169..92436
                     /gene="gshAB"
                     /locus_tag="IXT53_000240"
     CDS             90169..92436
                     /gene="gshAB"
                     /locus_tag="IXT53_000240"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HAQ4904103.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKKYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(92525..92977)
                     /locus_tag="IXT53_000241"
     CDS             complement(92525..92977)
                     /locus_tag="IXT53_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HAQ4904104.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            93127..94077
                     /locus_tag="IXT53_000242"
     CDS             93127..94077
                     /locus_tag="IXT53_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAQ4904105.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            94070..95029
                     /locus_tag="IXT53_000243"
     CDS             94070..95029
                     /locus_tag="IXT53_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HAQ4904106.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            95026..95781
                     /locus_tag="IXT53_000244"
     CDS             95026..95781
                     /locus_tag="IXT53_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ4904107.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            95804..96757
                     /locus_tag="IXT53_000245"
     CDS             95804..96757
                     /locus_tag="IXT53_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ4904108.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(97401..97697)
                     /locus_tag="IXT53_000246"
     CDS             complement(97401..97697)
                     /locus_tag="IXT53_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HAQ4904109.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            98052..98408
                     /locus_tag="IXT53_000247"
     CDS             98052..98408
                     /locus_tag="IXT53_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ4904110.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            98418..99317
                     /locus_tag="IXT53_000248"
     CDS             98418..99317
                     /locus_tag="IXT53_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HAQ4904111.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            99834..100565
                     /locus_tag="IXT53_000249"
     CDS             99834..100565
                     /locus_tag="IXT53_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAQ4904112.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            100710..102737
                     /locus_tag="IXT53_000250"
     CDS             100710..102737
                     /locus_tag="IXT53_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lectin"
                     /protein_id="HAQ4904113.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRINIPVENQRIGTFEGKLSWILADVPGN"
     gene            102845..103942
                     /locus_tag="IXT53_000251"
     CDS             102845..103942
                     /locus_tag="IXT53_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAQ4904114.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            104249..>104696
                     /locus_tag="IXT53_000252"
     CDS             104249..>104696
                     /locus_tag="IXT53_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ4904115.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRKL"
BASE COUNT        34016 a        18658 c        22188 g        29834 t
ORIGIN      
        1 ttcgaagaag cacgacaagc gtacgaaaaa caatggaacg aaatccatca aaattatgaa
       61 tatatcaatg caaaaagtaa ctttctcagc gccttttgga atgacggaga tgagcgctgg
      121 tgtgaagagt gtgacgatga tttacagcaa tacaagggac ttgcgttgtt gaaaaatcat
      181 cagcccatca cagtagaaag cagaaaggaa ttttatgaaa caactaattc tagcggaaaa
      241 accaagcgtt gcgaaagacc taagcaaggt gcttggtgct aatcaaaagc acaaaaatta
      301 ttatgaagga cccaaggtga tcgttacttg ggcccttggt catttattag gattgaaaat
      361 gccggaagat ctgaataagg aatggcagag ctggcaaatg gacacgttgc caatgatccc
      421 taaaaaacta ggaatcaaac cacttcctaa aacaggacat caattaaaag caatcaagca
      481 gctggctcag cgaaaagatg tctcagaagc agtcattgcg acagatgccg gaagagaagg
      541 ggaacttgtt gctcgctgga tcttagagta tgtgcatttc aataaaccag tgaagcggtt
      601 atggatctct tctcaaacag ataaagcaat caaaactgga ttcaagcaac taagacctgc
      661 gaaagaatat gatgcattgt atgaatctgc tcttgcccgg gcaaaagcgg actggctggt
      721 aggattgaat gtgaccagag ccttaactgt gaagtacgcg gataatttat cagcaggccg
      781 tgtgcagaca ccgacactag cattagtgag agaccaagaa aagaaaatcg agcagttccg
      841 tccacaaacg tatttcacga tcacgttgca agctggaaaa gaacaagcca aaatgcttca
      901 aaaaaatcaa tttgctttaa aaacacaaga agaagcacaa aaacttgttc aattgatcag
      961 tacaagcaaa ggaactgtca cagaagtcca agaaaaatta aaaacggaat ctgcgccatt
     1021 gccttatgat ttaacagaga tccaaagaga agccaatcaa cgctatggct attctgcaaa
     1081 gaaaacatta agtcttgtac aaagtttgta tgagacccat aagatcgtta cttatccgcg
     1141 tacagacagc aaatatctga caaatgacat gaaaagtacg atgaaagaac gattgcaagc
     1201 agtcagcgac tttgctcctg aggtcaagca gtatttgaaa aacggagcca tcgtccaaca
     1261 gacaaaagta ttccaagaca gtaaagtgac cgatcaccat gcacttttgc caactgaaaa
     1321 cagagcacgt tatgaaaaat taagtaatga agagcgaaaa atctatcaaa tgattgtctc
     1381 tcgtttttta ggactgtttg ctcaacccca taaagtcagc caaacaaaag ttacggttga
     1441 atttggtaaa gaacgattca tttttcggca aaatcgtgtc gtccaagcag gatggaaagg
     1501 cgaaactgaa actgagacgg aaacagtcaa atgggaaaaa ggaatgagaa tcaatcccga
     1561 tttcgccatc aaaaaagaac tatctgcacc gcctaaacca ttgtcagaag caagcttgct
     1621 tggtcaaatg gaaaaataca gtctgggaac cccagctacg cgtgcagaga ttatcgagaa
     1681 actaatcaaa tctgaactga tggaacgtac ccctcaagga ttgcaagtat ctcctaaagg
     1741 taagcaatta ttagtattgg taaatccctc tttagtaaca ccagatttga cagaaagctg
     1801 ggaaaaagac cttgaagcta ttgctgcagg aaagaaacaa gcaggaacct tcttgaatgg
     1861 aatcgaaaaa gaaacaaaac gcttggtgaa tgagatcaaa tcaagcaaac aagaatatca
     1921 agatttctcg attacccaaa agaaatgccc agaatgtggg gccaatctgc gcgagaaaaa
     1981 cacacgtgac ggcaaaatct atgtctgcac caattcagat tgcaactacc gcagaagaaa
     2041 agatccaaaa gtctctaatc atcgatgccc gcaatgtcac cggaaaatgg aaatcatcga
     2101 aggtaaaaac ggtgcttatt tccgttgtaa atacgatgga acgacagaaa aattgcttga
     2161 taaaaaagag cagaagaaga aaatgaccaa acatgaagaa cgacgcttgc tgaaaaaata
     2221 tgcacaagca gaagaaccag aagaaagccc attagcagca gcactaaaag cagcaatgaa
     2281 gaagtaataa gatatgaaag aggttgtgat agaagtggtc agcttcaaga aataagaagg
     2341 aatttacgaa aattgcttct caaatttttg tgaaatttcg gcttatttcc gaagaagcta
     2401 cttctggaac accatttatt cggaaaaaag gactgtgaca agacttttgt cacagtcctt
     2461 tttcatttaa aaaaatgttt acttcatttg acgtatatta ttggtgaggc ttcaattcat
     2521 ttggcttcac tttttctgtt tctcttatga tataaacagg acgctgtttc gtttctaaga
     2581 agattttccc aatatatttc ccgatgattc caagacataa caattggatt ccaccaatga
     2641 acaggaagat cgagaccatg gaaggccatc ctgatgtagg atcaccaaac aacaatgccc
     2701 gcaaaataat gaacaaaatt gcaataccag aaccgacaca agacaaagcg ccgataaatg
     2761 aagcaatcgt taacggtgca tccgagaagt tcacgatacc atcaatcgag tagttgaata
     2821 atttccagaa ggaccaagat gtttcgccag ccacacgttc gcggttttca taagaaatat
     2881 attctgtttt aaatccgacc cagctaaata atccttttga aaatcggttg tattctgtta
     2941 attctaggac ggcatccacc atttgacgag tcatcaagcg gaaatcacga gcgccatcga
     3001 ccatttctgt atcgctgatt ttattgatca actggtagaa ttttttcgca aagaaactgc
     3061 gtatcgcagg ctcgccttca cgagtcgaac gccgggtgcc gacacaatcg atatcagaag
     3121 tagtgatccg gttgatcatc tcaggaagca gttcgggcgg atcttgcaga tcaacatcca
     3181 tcacggtcac taaatctcct gtagaagctt gcaatccagc gtacagagcc gcttctttcc
     3241 caaaatttct ggaaaatgag acaaagcgga cataaggata agctgaggct agcttcttga
     3301 ttgctgctaa cgtaccgtct tttgatccgt cgtcaataaa caaatattcg aactcatggg
     3361 cacctttgaa tttttcaacc tcatcaaaaa aaagaggaac agttttttct tcattaaaac
     3421 atgggacaac aattgatact ttcatcgttt ctatcctttc aatcgtatgt ttatatatgg
     3481 tttacattca aaatttaata aaccttaaat aacaccttta aaatttgatt ttacgtaaaa
     3541 gaacaggtat tctttttgta aactaacgta tttagtttac catgtttcct cggtttatca
     3601 aacttttaag aacaattttt attagttggc aagtagtttt ggatagagaa aaaggaggaa
     3661 gttccagtct attgactagg agcaaacaaa atgaaaatac acacacctat cggcttttta
     3721 tggttagatg cagatgaatc cggattgact gctgtatctt ttcagccaat ttcagaaagc
     3781 gaatcagcga atcagcgaat tttaacgcgc acacaagaac agtttgaaga atattttgcg
     3841 ggaaaaagaa aagaatttga tgttccttta tctataaaga gaggtacgcc ttttcagcaa
     3901 aatgtctgga aagctttgtc gaccattcct tatggagaaa tccgaacgta taaagaagta
     3961 gccgttgctg ttgatagtcc gaaagctgtg cgagcgattg ggcaggcaaa tcgaaccaat
     4021 ccattgccaa tcgttattcc ttgtcatcga gtgatcgggc aaaatggaaa actgacagga
     4081 tatatgggaa acgcaataga tggggttgaa atcaaacgcc aattgttgtt gttagaagga
     4141 tatctgcaaa attaaaaaca gttgtgattc ggacattgtt ccatgcgaga atcacaactg
     4201 ttttttcatg ttcattctaa actttgatca gccaaataaa tagtaaagcc atcactgttt
     4261 ggagaagccc gacagctaat ccataagata agaagcgtgt accttctttc atgaattttg
     4321 caaagtctaa acgcagacca attgccgcta aagcagtgat ctcaaaccag ctgctgataa
     4381 aatgtgccga ttggtcaata actacaggta aagaaaacaa gctgttgacc aaacaaaaga
     4441 tcacaaaccc taatacatac caaggaagcg gtaattttcg ttttttctgc gtatcagttg
     4501 gcgtttcaaa cagagctggt tgtttcttgt gcatgaattt ttcaaacaga tagacaacaa
     4561 caacgagcat cataatacgt agaatcttaa atagcatggc gaattcgaca acagaattgt
     4621 caagtaagct tgccccagcg actacttgac caacagattg gagtgtacca cccaaaagtg
     4681 cacttttttc aatgagattt ccaccaaaca actgtaatcc aagaaaaggg agagtcaaca
     4741 tcatcacagt acccagtaga ttgactagtg tgatgatctg tcctttttct tcctctttcg
     4801 catggatgga aggggcgatt gaagcaatag cagaagaacc acaaacagcg tttcctccag
     4861 ccatcatcaa agaagaagta tcgctgaatt gcagtttttt accaatcaag taagcaccga
     4921 tgatagtgac tgacatttgc agtaacacaa aaacaattcc ttttacacca atttgagcaa
     4981 tcgtttgagc tgtgacagtt gcacctagta aaaaaacaga gaattctaat agtttgcttt
     5041 cagcaaattt tgtgccgatt gctagaacag gctgtttaaa aaaagtattt cctaataaaa
     5101 tacccaataa aatagcaata gtcgctccac ctaactgtgg cagataaata gcaagaagcc
     5161 tgctgaaggc agcgatggca aaagaaagca gtaaaccggg taagatttcg cggaccgctg
     5221 aatcctttac agattttatt ggatgtgaca atgatttcat ttgatcggtt cctttcgtca
     5281 ataaatacta aaagcatact cgaacatagt agcactaaac cagtattttg acaaaagaac
     5341 aagaggtcgt gacaaaagtc ttgtacgacc tcttgtttcg aataaacggt gttccagaag
     5401 tagcttctac ggaaataagc cgaaatttca caaaaatttg agaagcaatt ttcgtaaatt
     5461 ccttcttatt tcttgaagct gaccacttct gtcacaacct cttattttga ttagaacttg
     5521 cgaaaataaa ccggttaaag tgtgtctagc gcaagagatt tgctataata gacgataagg
     5581 gtaaaatgta acgagaaggg gttgaaaaat gtgtatcaaa ttgtaacaga ttcttgttgt
     5641 gatctgccat accatgtatt agcaaaagaa aaagttgatt ttttatcaat gaatctccaa
     5701 ttgaatgatc aagagttagt agatgacctc ggaaagacat tcaattatga ccgctacatg
     5761 aatcagctga aaaatggtgc gatgcctacg acatcacaaa tcaatgttgg ccgatattta
     5821 gaatttttcc gttcttatgc ggaaaaacag attcctgttt tatatgtggc tttttccagt
     5881 ggattaagcg gctcttacag tagtgcgctt caagcggttg aattgctgaa ggaagaatat
     5941 gaagaccctg agatctatgt ttttgataca aaagctgcaa gtattgggga agggctttta
     6001 gtattggaag ctgtaagatt gaaaaaagaa ggtaaaagca tcgaagaaac cctagagtgg
     6061 ctggaaagta attatttgaa agtccattca tgggtaacag tagacgatct gaaatatcta
     6121 gaacgaggcg gtaggatttc caaagcaagt gctactcttg gcagtctatt gagtgtaaag
     6181 cctattatta cggtggataa agaaggaagg ctgacgaatg ttgacaaagt tcgaggaaga
     6241 aacaagtcga tccagacaat cgtcaatgag acggtaaaaa caatcgttgc accacttgat
     6301 caaatcgttt atgtggctta cgctggggat ctagagagcg cggagaaggc aaaacggctt
     6361 ttggaagagc agataaagat gaaagatgtg aagctttatc ccttaggacc aacgatcgcc
     6421 agtcacacag gctatggctg tatcgcgatt ttttcgatgg gaatcagtcg ataaaagcag
     6481 taactcattc tgtaaaaaag cccagaaatt gattgtaaca aaaacagcga ctcttaaaag
     6541 atagatgagc atcctatctc ttagaggtcg ctgttttttt attttttaag ctaaataggc
     6601 cattatgccc ataactcgtt caattgtttt tgaatatctg cattttccaa aaattcatca
     6661 tatgtcgtat cagctcgatc gatcacacct ttatccgaaa cggcaatgat gcggtttgcc
     6721 aatgtttgga taaattgatg gtcatgtgac gcaaataaga ttgagccagt aaatgccatc
     6781 aagccgtcat tcaatgctgt gattgattct aagtctaaat ggttcgttgg atcatctaat
     6841 acaagtacgt tagctttcga aagcatcatt tttgaaagca tgcaacggac tttctctcca
     6901 ccagataaaa cattcactgg tttcaatact tcttcaccag aaaacaacat tcttcctaag
     6961 aagcttctta agaaggtgtt atcatcttct tctttacttg catattgacg caaccagtct
     7021 aaaatcgtta gatcagtatc gaattctttc gttgtatcct ttggtaaata agaattactt
     7081 gttgtcacac cccaacgtac tgttccagta tctggcgtaa tttcacccat gataactttg
     7141 aatagcgttg ttgttgttat atcattttct gcgacgaaag cgaccttgtc ttctttgctc
     7201 aaattaaaag atatatcatc taaaattttc tttccatcaa tcgttacact tacattttct
     7261 acctgcaata agtcgtttcc aatctcacgt tcaggtttga atccaacgaa tggataacgt
     7321 ctagaagaag gttggatatc gtctaaagta attttttcaa gcattttttt acgtgaagtt
     7381 gcttgttttg attttgatgc attcgcacta aagcgggcaa taaattcttg gagttctttg
     7441 atttgttctt cttttttggc atttgcgttt gcttgaagtt tagccgctag ttgactagat
     7501 tccaaccaaa aatcataatt tcctacataa agtttgattt taccaaaatc tagatccgcc
     7561 atatgtgtac atactttgtt caaaaagtga cggtcatggg atacaacgat cacagtattt
     7621 tcaaaattga tcaaaaattc ttccagccaa ttgatcgatt ggatatccag accgtttgtt
     7681 ggttcgtcta gcaaaagaac atcaggttta ccaaatagtg tttgtgctaa taacactttt
     7741 accttttgtc caccagttaa ttcactcatt tgctgatggt gcaattcttc agggatgttc
     7801 agaccttgca gtaaaacagc tgcttctggt tcagcttccc atccattaag ctcagcaaat
     7861 tctccttcta attcagctgc tcgaatacca tcttcgtcag tgaaatcctc tttcatgtaa
     7921 atcgcatctt tttctttcag cacttcatac agacgcttgt gtcccatgat gactgtttca
     7981 agtacagtat agtcttcata gtcgtaatgg ttctgtttca atacggccat ccgttcatct
     8041 ggtcccatag taacggaacc tgtggaaggc tgcagatccc ctgataacac tttaaggaac
     8101 gttgatttac ctgcaccatt tgcaccaatc aagccatagc agtttccggg ggtaaattta
     8161 atattcacat catcaaatag tttgcggtct gaaaagtgca aactcacatc atttacagta
     8221 atcaattttt ttcctcactt ttatcattat tagttttgac aagtcttata gaattatata
     8281 ggggaaaggt aaaagattca aggcaatcga acattctttg tgtttcacgt gaaacagaat
     8341 gaaaaaaata aaaataaaag cggagcgaaa atagctacta taggatttat tagtttgcaa
     8401 cagcataatt tttgacgtta tttataagag ggagtactat aaaactgtaa aagaaatgaa
     8461 agaaggattg aaaatggatg aaaaaagaac catcgactgg ggctcattga ttttgggaat
     8521 tttatttgta cttgtctcat tgttatcatt tcaagaccct gtcggaaatt tagttgcaat
     8581 tgtagtagtc tttgcgattt ttgcctttat taaagggata tttgaactgt ttgtacggaa
     8641 cagattaaaa gagctaacag gatataaagg aaagatgccc ttaataatcg ggatcatcga
     8701 tattctagtt ggcgtctttt tcttattcaa tattggtgca ggagtcgcag cattgccttt
     8761 tgtttttgca gtatggttta ttgctgattc agttttagcg ttatttactg ctgacttggc
     8821 aagaggagta agtgaaggat attattggtt tacgatcatc gtcaatattt taggaatttt
     8881 attaggaatt ttcttattgt ttaatccaat ttcttctgcg ttgacattaa gctttttagt
     8941 tggattctat tttatgttat ttggtatcac acatatcgtc tatgcattta gataagtaaa
     9001 atcaaacaaa taaaaaagcc ggcttccgat tacagtttta ctgttgtaat tggaagccgg
     9061 cttttttatc atggacaaat atgcacgagg tgatttttag gtgtcgagac aaatttatgc
     9121 aaaaatgtga gaagctatat acacaaattt tatcttaata ctcgaagcat agcgcagttg
     9181 tcacaacctc ttttcatata attcattcaa tactggcaaa tcatggactt catatttttc
     9241 tttgaatgct tggatttctt cttcagaata ttttcttgga tctaaatgca gatcttctac
     9301 tggcaatggg cgcttgttat tgatcttcac atcgattact acaggacgat ctgattttgc
     9361 agctgcatca aaggcaaatc gaagttgcga gtattctgtg actgtaaagc tatttgcccc
     9421 gagcgcttct ccaactttac cgaaatctgc atcttctaaa aatacaccaa atttcttttg
     9481 ttccgtatct tcttgttctg cttcgatgaa accaaaagaa tcattcgaaa agacaacatt
     9541 aataattgga agctgatatt tgacttgtgt aatgatatct tgcatgacca tagcaaaccc
     9601 gccatcacca cttaaggtaa atacttgttt ttctgggtag cttagttgtg cggcaattcc
     9661 tcctggcaca ccattaccca tagttgcgaa ccatccagat gtcgtatgtt tttgtttacc
     9721 gttcatattc aataaacgaa tagaatggat agtcgtgtta ccgacatctg tgacgaaaat
     9781 cgcatcgtct tcagcaatac gattgatttc tttatagaca gcctctggac gaatcggttc
     9841 ttcctctcga tcttcaaaag atttcaacca atttacccat ttttctttat tttcttgatt
     9901 tgctaggtac cagctatcag cagggcgagc atcccctaac tcgacaagtc tttgcaaagc
     9961 tgtgtttgca tcaccgagga tcgacaaaga tgtttcatgg cgtcgtccaa attttgacgc
    10021 atcgatatcg atttggataa attcagcagt tggattaaaa aatgcacgtc caaatgggaa
    10081 gtcgctaccg acaaataaga tcagatcggc ttctgccaat gcttcatttc cgggtttagt
    10141 agcgacacgt ccagcaaaac caagaaagtt ttcataatca tcaggaataa ttccttttgc
    10201 tagaacggaa gcgacaacag gaactgaaaa atgttctgag aattctttga tttgcggaaa
    10261 tccgtttcgc gttccttgac caacataaag aactggtttt tttgcttttt caagataagg
    10321 caaagcagct agcaaatctt tttcttctgg taatatcaca cttgttttgt gagtgtgagc
    10381 ggtggagaaa ggttgttcgt caatttcttc aaaaccaaga tcaacaggga tagtcacgat
    10441 agccacgcct ttatgctcat aggcggcttt aatggcttca tctactacat gcggaagact
    10501 ttgtggtgtc atgactgtac gattgtatac gctaacatct gcgaaaatag gattttcatt
    10561 tagttcctgg aaagagttat agttcatgga tgtagaagca acttgtccta agaacgctac
    10621 tagaggcaca tggtccatct gtgcatcata caaaccattg atcaaatgtg tcgcaccagg
    10681 tcctgcagag ccgaagacag cgccgacttt ccccgttaat tttgcatctg cagcggctgc
    10741 gagtgcgccg acttcttcat gacgaacttg aatgtagttg atttcggttt tttcttgata
    10801 tagcgcatcc ataattgaat tgaaagatcc tccgggaatg ccgtaaatat ggtcgatacc
    10861 ccagctttca agaactttta ccattgcgat tcctgcattg atttttgaca tggtgtttca
    10921 ctcctttagc aatgatgata cataaatttt ttgtatctag agtatagaac tttttatcga
    10981 aaatcccaac taattcgtct tttcacaaaa atgtagtcat gtttttggga atatgctaga
    11041 gtagtacata gatagattca agagagggga atggcatggt tgaatggcta ttgagacttg
    11101 aaccttggca gcaagcactt gctgggacag cttttactta ttttatgaca gctcttggtg
    11161 ccgggttggt ttttttcttt aaagaaatca aaaaagaagt gctaaacttg atgttaggat
    11221 ttgcttctgg tgtcatgatt gctgcaagtt tttggtcttt gttagatccg gctattacaa
    11281 aagcagaaga aaatggagat attgcttggc tagttgtaag tattgggttt ggtttaggcg
    11341 gtgtgttttt atatatggca gataaaacac tgccccatat gcattttgga ccacaacatg
    11401 aagcagaagg attgcctacc catttaaaac ggacgatctt gcttgttttt tctattacat
    11461 tgcataacat tcctgaaggt ttagccgtgg gggtagcatt cggtgcagca gcaactgcgg
    11521 ataatccgac agccgctata ttagccgctg tttcggtggc gctaggtatt ggtatccaaa
    11581 acttcccaga aggagcagct gtatcaatcc cgttgcgaca agaaggtctg agtcgtaaaa
    11641 aggcctttgt ttacggacaa gcttctggaa tcgttgaacc aattgctgga gtgattggag
    11701 cgctactagt aacgaaagtg gagctgctgc ttccttatgc acttgctttt gccgcaggag
    11761 caatgattta tgtcgtggtg gaagaattga tccctgaagc acagcagaca ttgtccagca
    11821 aacatcattt tgcagttttt ggcgtcatga gtggatttat cattatgatg attttggatg
    11881 tggcattagg ataaataaaa aaaatatata tgatatatca aatagggccg cgaacttata
    11941 tcgcgaccct atttttttag taagttatat cataatgaag tgaatggtat catacattaa
    12001 taatagtttt ttctatgtgt atttcctttg atccagcgaa caagccagat gataagtgcg
    12061 attgggatta gaactttgac cgcaaaccat aaaagcccgc ttaggacact gacaaaaaga
    12121 ctgatgacga agctagctgc aattagcact aaaagaacca tgaagatatt taagaacata
    12181 tttttttgac gcatacaaaa ccctcctctt gttcattctt tactatagaa aaagtataac
    12241 agggattgtt taaaaacacc tccaactgaa gactgatttt tacttcatct acttaaatga
    12301 tcaaacaatt gctttttctc tggtttatca tctaaaatag agcggaaaag gaggagaata
    12361 tcatgcctaa aaatcgtctt gaagcattta cagatgctgt aatcgcgatt atcatgacta
    12421 ttttagtttt agaacttcat gaacctaacg gagacacgtg ggaggcgctc gctgcattag
    12481 gttaccgatt ttttatctat attgtcagtt ttatcatttt agcgatttat tggaacaatc
    12541 atcatcattt atttcaagtt gtacataaga taaatggacg ggtcttatgg gcgaacagtt
    12601 tctttgtttt tgcgctttcg ctttttccgt ttgcaacttc atggatcagt gagtacatca
    12661 atagcctggc tccagaaatg acttatggtt ttgttacctt gaccgctaat attgcgtatt
    12721 atatcttggc aaaagaactt gtcaaagcaa ataaaggcca cgagaaagca ttgaaggcga
    12781 tgttgaataa ttacaagaaa tcgtatctat cgattggttt gaatgtgtta ggaattgtct
    12841 tagggtattt agtagcgcca gtagccgtgc tgatcgcaaa catcgtgatt ctagctgctt
    12901 ggatcatccc aagtcgtacc atcgaatcac aatatcggta aaatgatccg tgtttaaaat
    12961 ttaagagaaa aatatagaaa aaataaataa acgatcaaat atccgagtga tactagtatt
    13021 caaagaggga tgcagatgga tcaaaaaaga attcatgtag gactgattac tagttttgtc
    13081 acgatgctta tttatttgga tcaaggtcat ttgacttgga cagtagttgg tgtcgtagga
    13141 tttttgacat tattcacagc aataaaaaaa taaacatctt tatgtaatct gatgttctat
    13201 ctatttaaga aaagcatgtt tacagcagta tgattctgtt gtaaacgtgt ttttatttgt
    13261 ggaaaattta atttttagaa aagtgtatcg aaaaatagat agttagtttt tccacagtgg
    13321 ataacttcgt aagagataaa aataaaaaat tagtcaaaga agggcgtata gatttctttt
    13381 gataggagat atacaacaga aatgaataaa tattaacgtg ttttcaaaca gtataaaacg
    13441 gtgtggtttt tggaataaaa aaataacata tctcatttta cacttttgtt attttatact
    13501 taaaaaataa cgacacagaa aataccgtgt ctcagatttc tctactactt ctaaaaacta
    13561 ctgttttttt cctttgaaat gccttcaaaa agtaggatca ggcagaatat actggcagaa
    13621 tcaaggagaa gggagcctaa ataaggagaa aaaccataga aaatataaag taattactta
    13681 taaaacaagc tttttataga aaaataatat acctgagtag ggtataaatt aagacggaat
    13741 tcctttctag attgagatat tgactaggaa gaatgttaag attttaagcc tttaagctaa
    13801 aaaaaaaccc tttttgttaa ataaggggaa tatagcctgt cagtttcaaa ctgcaagtcg
    13861 tcctgctgca ataatatagt agattagaga tgaaaataga tttttttaaa ccaatgactg
    13921 aataaaaaga ccgtcataga tacacattgg aatgtgtcta ttacggtctt tttttacgaa
    13981 cgattttatt gaagacggac agttcgggtc aaaaataaaa aagctacgat cagaaataag
    14041 aataaaatgt atcccaaaat aatggctaga cggtctaact tatttgtcga tagatgtttg
    14101 tttagaagtt gataggccca taaacctatc agtcctcctg ttgtattagt tatcaaatca
    14161 gtgatgtccg ctacgccaat accgaatagg aattgaatca attctgccaa gaagcttaca
    14221 aggaaaataa tccctaattt tttagaaaaa gaaagtctct tgaaattgac attcaaaagc
    14281 accccaaaag gaaaaaagac aatcaagtta taagtggtct cacgtaatac cgtcgtattt
    14341 tcaaaagaaa aagtagaaaa agggacgaga ttgacattgc tgtaagaata agtgagaaat
    14401 tgtggaagat taaatgatag cttgaataaa atcaaccata atagaaagaa taaataaatc
    14461 cctaataaaa attttgatac ataccgcata gacatctcct ccatttaaaa agtataccat
    14521 cgaaaaccgc tgaaaaatca taaaaaagta tgaagtttaa ggaccaacgt agttatcgta
    14581 cgaaaattag aagctttttt ttgttgggaa tccggtttct ttaacaaagc taagatagcg
    14641 agttgcttgg aaaagttgct atcttttttc tgtcaaaacg actcggaatg ttcgtttttt
    14701 gttctaattt ctccaagttg tttacttttt gatactaagt ttatgaaaaa tcaagaaata
    14761 gttgttatac taggtaatgc tgacaaacgg gattggcatt tcatcttggt tcagcttttt
    14821 ttccaattac taggaaggag ggatagattg ttcaagactt attttcgttt caataaaata
    14881 caagataatc cttttcgtat catgaatctg atgctactta tctttattgt attatggtta
    14941 ggttacataa atcatgattt tttgatttca agtttttcat cattagggat ctttactttt
    15001 ttctattatc aaaacatacc gatgaaatat ttgctgaaac gtatgtttct gatcggttgc
    15061 ggactgttgg ttgctttcgt tttggggatc ttatcgactt atgttttatg gcttgaacca
    15121 tttgccgtag ctttggttgc tttttccagc agattcgtgt tgcgattatt ccatatttct
    15181 aaaccaggtg gcttgttttt tgcgatgctt tctgccatgg gaacgagcat gcagctgcca
    15241 atcgctcaat taccgatcgt ctcactttat ttttttatgg gtgtcgtttt tgccctgatt
    15301 gcggcagtga ttacaaaatt actggattca cggcctgagc agacaattga aaaagcaact
    15361 ttaaaagaaa gatttcacga agagccttta gtgatcattg attctgtttt ctacagcgct
    15421 gccttatttc tttccgttta tgtcagtcac ggcttgaatc tgcataatcc ttattggttg
    15481 accttgtcat gtgcatcgat tttgctagca gaaaacttgg atgcgatgaa gcatcgacaa
    15541 gtccaatatc tgattggatc gatgggtggg ttatgtgtgt cggcattctt gtcatttgtg
    15601 ccgtttacgc agttgcagac catttttctg atcacttttc tatatggaat cgcacaattt
    15661 ctagttgcta gaaattatgc agttgctaat atttttctta atccgatggc gttgatgcta
    15721 tctacactta ttcgtggagc atacttgatt tctttgatcg aatatcgttt tttagggatc
    15781 gtcataggaa gtttcatcgg tttaggtgtt gcgtgggtaa tgacggttgg tttgcaacat
    15841 tatttaacgg ttgttcgtca aaaattagaa tagaaggaca aatatatgtg ccaaaagagg
    15901 ttatgacaag atttttgtca caacctctaa ttcaaggtgt tcaaaagcca gattctcagc
    15961 attaagtcaa attcacacaa aaatgtggaa aggaactttc aaaaaaattt tatatttatt
    16021 ctatcaaaaa attttgttta tgatggtaac tgaataaact ttcctgagat ggtcaatgct
    16081 ttccttgaaa gtcttacaga tattctttct tcatagaaag tttaagtttt atagttagtg
    16141 tacaataaaa gtatgtttat accatatggt gtccggataa tcagtcattt aaacaatgag
    16201 gttcaaagat atgaaaaggg gaaatttgat gaagaaaata agcaatgaaa tccaaattct
    16261 ggcagaaact ttaactcttc ccaaagtcgt tatagagcaa atgattgtac aagcaaaaaa
    16321 catccttgat gaggtgtttg agcaatatag tcatgaacta atggatccta ataaagcaaa
    16381 ggcgactgca aagaaaatag acaaagtcta tgaagaggaa ccttttgcag cattagctat
    16441 ttatctatat gcagcttctc aaagttggaa aaaaatttat caaccattga agattccaag
    16501 aactatctat ttggcaacaa tgaatgcttt tactagattt attcatgaac atttccaagt
    16561 aacaggaagc tatcgctttg accgtggttt ttggatttgg cattacacta gtggcttgat
    16621 ctttcggatt ggtgaattgg agtacgaaat gacatatttt ccgaaagggt acacggatct
    16681 aagattagaa ggaaagcagt gcctttctct tcatattcct tccgatgcag atttatctca
    16741 gaataagata agagatagtt acgaaagtgc cattcgtttt tttgaacttt attttcctga
    16801 ttaccattac caagtgatgt acacagatac ttggttacta tcacccaatc ttactaaatg
    16861 gttgaaaaaa gagtcaaaaa tctgtctttt tgcagccgat tatcgattat tatcagttga
    16921 tgaacaggac gatagtggag tcccttggat ctttggacga gtagatgccc aaatacatga
    16981 ttatccagaa agtacgagtt tacagcgaca agcaaaagag caattgcttg caggcgagca
    17041 cattggctct ggcttaggta ttcttacttc ataagtttag tacttcgaaa tagtttaaaa
    17101 gagcgtgaga caaaagtaaa gaacactttt gtctcacgct ctaaacacgt ataaacggcg
    17161 gaagcagaag caactccttc ggaaataagc tgaaattcac aaaaatttga aaagcaattt
    17221 tcgtgaattt cttcttattt cttggagtta aacgcttctg tcccagcctc ttttttagtg
    17281 ttctatcgaa cacacagtac gttcaatagg taaactagct ataacaatag atattaaagt
    17341 taacggacaa gagggccttt tttaggtagc taaaacgtaa taatgaaaat ttatttccag
    17401 agataggagt caatcagcta gtctgatcga ctgttttaac aagttgactt tcattttaac
    17461 ttttgatacc atttaaaaca caaatcgtaa ttgtttcgat ttgtgttttt tagcatgact
    17521 acaatcaaag aagaaagtgg gtcgtccata tggaaaaaac agatatctca agtgcatacc
    17581 gccgcttgaa aagcccaaat atcaaaacac gaaaacgtgc gctgaagatc atcaaagatg
    17641 tgaaacggaa tagtgggaaa aggtaagaag aaggaaactt aattaaaaaa atagccgaaa
    17701 aacgtttttc ggctattttc aataatcggt ttttgtgtaa ggtttccgaa agaactgctg
    17761 ttgattttcc atttttaaca tatgataaat tggtagaatc tttcttttca aattgatacc
    17821 attccctaat tccagttata tagttaacta cagaaccaca aagcacgtac gtttataaaa
    17881 gtattatctg tagttatttt tcaattccct ttcaacctct tattttgacc agtagtattg
    17941 atcactttac cttttaacac ctttgttcgt ttttgaaaca tttttagtca agaatggaaa
    18001 cgtaactaca taatcaaagg ttttgcgaaa tatttcatga aatatttcat gtagaagcga
    18061 gcaatacggt tgcaaataac cattatcgtt taataagcag ttacgaaagc tcccaatatc
    18121 tctaacgttc agctcactcc atttcttttc ttcaaaaaaa tcagtgattc gtatctcttt
    18181 ctatttgtcg aaaaagttaa ctttaaaaag taacgccgaa aaaatatgca aaaaaataaa
    18241 aaagcagtta ctattgctag aataacatca aggaataata agttttggtt ttcagataat
    18301 agtcgataag ttgtgtattg gaattttaga tacagtaata aagtattttt catgaaattt
    18361 tgtgagaatc actattacaa gtatctttaa taaaattaat ggttaaggag gaacttaaag
    18421 tagaatgtaa atgaatagtt gtagtcagtc gattgtgata ctagactcaa agttgcagga
    18481 ataagttttt caatttgtga ttggaagata aaaaatgaaa aagaagggct tttttttata
    18541 ctttgttgat tgtagtgttt attttccctc tgtttagcca agtaacattt tctatgactc
    18601 aaccagtttc aagtgaggaa catgataacc tttttcgtgc tacagaagag tttaagaaat
    18661 atcaggaaaa tattgttccg gagactccat tagctagtga gccaattact gatattgatg
    18721 gaaaccaaat aggatggtat atacagtacg aagttatggt ggtaaataat gactcaatag
    18781 taaaaaatac agtaggctta gagctaacac cgattgaaaa ggtgagttca ttgttaacgt
    18841 atatttatac tgatgaaagt gacgagactg agatcatttt aacggattac tcaaaaattg
    18901 ctactgataa aaagtatata ttgtggatgt agacactagt gaaatacttt atgatataga
    18961 aattgatgaa aatttactag atgtagcaca agaaatcgat catcaaaagc aggaagctat
    19021 tcgatatgct ggtagaaaag ggaaaagaaa atatttatta cgtgaatgta gcaaaagtta
    19081 gagaagatga aaatgagtgg aaagaattca agagtcgcta ctcaattaac tcgactccga
    19141 catttacagt ttacagagaa ggatcgatag aaaaaactgt tttttggact aaagaaagtg
    19201 gaatgtcttt agctgaagta gaagagtttt tggattatgt tagtatgcaa cagtaaggga
    19261 gttcttatta tgaaaaattt tgacacatat tatgttattg ttttttcgat atctttgctg
    19321 ttaatattac taactttaat gcagtataaa gagtatccag agtattttat tttaactttt
    19381 gtatatttag taagtagcta cacacttcta tataaattca gaagttcttc tagagtttct
    19441 aaagtttgtt tcttaattag cttattgatt ctataatatt tttgatttat ataggaaatt
    19501 aaaaatatta tagaatcaac actcagagaa cgggttggtt tttaatagat ttaataagta
    19561 ctgataatga aatttctcct ggttgggaaa acgaagttgg ttaccctgta gaagaatttt
    19621 ttcaagatta ctttgcggag tatgccgata ggataatgag agattacgta acagataaaa
    19681 ctcaagaagt catcaatcaa tcccagtcat taaatgaatt atctgataag gcgaaagaag
    19741 aagcagttgc cattatttct tcagggatta gtgactatat tgatatgagt ggtgaaatca
    19801 attatggcga taaagacaaa attgtacgca tgataaaagc ttttatcaaa acttttgatg
    19861 tgaaggcaga tcttaagttt aatgatactt acctagtagg aaaattaatt aatatccttg
    19921 ggacagagaa agatacggaa acgtttattt cctctttgtc tatgttgttt acagataagc
    19981 atttttcaac tcagcttggt ggagaggaac tagtggaaat cttttaacca ttacgcccag
    20041 cattaataaa agtatatcaa aaaagtactc ctgatgattt ttaagattgg tttgagaaat
    20101 aatgtgaaaa cactattttt aatgttaatt atagatagag atgaacgcgt catatggtta
    20161 aaagaatgaa aaagtcaaag atacactgct atatttcact aaggatatag cagtgtatct
    20221 ttcatgtcgt ttcactggca gttttttata aaaggaggtg tgcatttcag ttgcttttct
    20281 ttggtagtta gttgaatcgt ttaagaatta acggatgtgg cagacaggtg ctctgtccaa
    20341 aaaccagtaa aatctttgat ggttccatct ttatttagct gtgcctgttc aaatactgtt
    20401 ttgtttttac cgaatggatc aattgcaata attgttccga tccccgcacg tttggcagca
    20461 agtaatcctg agtaagcatc ttcgataaca agacaatcag ccggagctaa gctcagtttg
    20521 tctgccgctt taagaaaaat atccggttgc ggtttcccag ggaaagagcc gtcgtcatag
    20581 acaatcttgt cccagtcaaa ccagcgggct aaatcgaaaa tatcaaaata aaaagcaaca
    20641 ttctctttga cagtagccgt ggcaatcgtt aatggaatct gttggtcagt cagctggtca
    20701 agagtcgctt caagcccttt tgtcaactga agctcgtttt cattttgcaa gcaaagcata
    20761 cgataatagc tttctttttc ttctgacaat acggcgatct cagaggcggt caaatgctct
    20821 gaaatgaaat gtttcaaaat ctccgtattc gtccgaccat gaatattttt taaaatatcc
    20881 tcgtctgata aaccatcttt tgtatgatca tgtatcatat gcagccaagc agcttcatgc
    20941 agatgagagt ccaaaaacat tgttccgttg aaatcaaata taatcccttt catagttacg
    21001 ctcctatagt ggtattttcg gttgaatcag caatttgcag atcgacatat tgtgcatagt
    21061 gctcttgaac ctcccgcgag atctggcgat ccgtaatgag cagatcgaaa tcatgcaaat
    21121 tataaaacac ataaaaatca tctcgattga attttttatt atcaacgagt agatatttct
    21181 tacgggaatt atttagagcg atctgctgtg cttctccttc ttcggtactg taagtagaga
    21241 tcgcttcgtt gtgaacgcca tttgaactga taaatgcttt tgtaaacttt agtcttctta
    21301 aattgtcgtt ggtgatcggt ccgacaaatg cgccggtatt tttccggaaa tcaccgccaa
    21361 tcagtagaat ttctggtcca tctgcttgag caagaatatc aaaaactgga taattatttg
    21421 taatgatccg aattttcttg ttcagtaaat gcgtcgccaa tagttcgatt gttgtacctg
    21481 gtcctaagaa aatcgtttct ccctcgtgaa tcaaggaggc tgctgtggca gcaatttctt
    21541 ttttttcttg aatctgcaga gtggcttttt cagtgtgaga aagttcttga tctaaagtgt
    21601 aggaaagact ttgagcccct ccatggacac gcagcagctt gcccgatttt tctaattcat
    21661 ctaaatctct gcgaacggtc atatcagaaa catctaattg attgatgatt tcattaacgg
    21721 taaggattcc ttttttattg accatttcta cgatagtaag caatctttct tttttcaaca
    21781 ttgtcttcac ctctaattta aggataatct atcatataaa cggacaacag tcaatctaat
    21841 tcgaacaaaa gtaaacattt agggtggttt ttatgtttgt tacaatagat tttattgctg
    21901 ttttctggtt tttatgatta tttgtgtttg tttttgttga caaatgtttt ggatgcgttt
    21961 acaatgcaat cagttagaaa gagcgaaaac tgattataga tttgaggaga gatgtacatg
    22021 cttaaattac cagaagattt tatttttggc ggagccacag cagcctatca ggttgaagga
    22081 gccactaagg aaggtggcaa aggcgctgtt gcttgggatg actttttaga ggaacaaggg
    22141 cgattcagtc ccgatccggc aagtgatttt tatcatcaat atgcaaagga cattgaattg
    22201 tgtgaacgtt ttggtgtaaa tggtctaaga ttatccattg cttggagccg catttttcct
    22261 gatggagctg ggaaaccaaa tccagaagga atcgcttttt atcatcgtgt gttcgaagaa
    22321 tgtaagaaaa gaaacgtaac gccgtttgtg acgttgcatc attttgatac ccctaaacgt
    22381 ttgtttgacc atggagattt tttgaatcga gaaacaatag aagcatttgt ttcctatgcc
    22441 attttttgtt ttcatgaatt caaagaagtc aaggtgtgga gtacctttaa tgaaatctat
    22501 ccagttgcaa ccaatcaata tttgttaggt gttttcccac caggaatcaa atatgatttt
    22561 acaaagatcg tcgcctgtct tcataatatg atggttgctc atgcccgtgt agtgaactat
    22621 ttcaaagaaa acgaacttcc gggtgaaatt ggtgtagttc actcattaga gacgaaatat
    22681 gcggcaacag acgcgccgga agataagcat gctgcctttt tagacgatgc attgtcgatc
    22741 cgtttcttat tagatgctac ctatttaggc tattattcaa ctgaaacctt gactgctctt
    22801 gatgagatat gtgaagcaaa tcaggccagc tatcattttc ctgaagaaga tttcgttgaa
    22861 ttgaaaaaag catccacgcg taatgactac ttagggatca atcactacca atgccatttt
    22921 gtcaaagctt acgatggaga aaatgcaatc catcataacg gtacgggtga aaaagggacc
    22981 tctgtttata aagtgaaggg aatcggagaa cgtatttata aagaaggaat tcctcgaact
    23041 gattgggact ggctgattta tccagaaggt ctatatgact tgttgctgcg aattaaaagc
    23101 gattacccac attacaacaa aatctatatc actgagaacg ggatggggta taaagatcag
    23161 tttgaagatg gcattattat ggatcagccg agaatcgact atttgagagt ctatttggaa
    23221 tccttaagca aggcgataac agccggggta aatgtgaagg ggtatttttt gtggtcgctt
    23281 atggatcttt tctcatggac aaatgggtat aacaaaaggt atggcttgtt ttatgttgat
    23341 tttgaaaccc agaagcgtta cccaaaagaa tcagcttact ggtacaagtt ggtcagtgaa
    23401 accaaaacga ttatttaata agtgataatg gaggaagaaa agatgaataa agaggaatta
    23461 caaatgcttg gttttgagat cgttgcatat tcaggcgacg ctcgcagtac attattgaaa
    23521 ttattgaaag aagtaagaca aggaaacttt gaacatgttg attcggcgtt gaaagaagcc
    23581 gacgaaaatt taacccttgc acataattcg caaacaaaaa ttttagcgga agaagcatct
    23641 gggaaagaga tggagatggg attcattttt atccatgggc aggatcattt aatgaccact
    23701 ttattgctga gagatctgat tcaagacttt attgtactgt acagacagaa tcaaggatag
    23761 gagaagaacg ctatggatgg aatcattaaa caaatcgaaa aagggaaacc attttttgaa
    23821 aaaatctcac gtaatagtta tttaggagcc gttcgtgacg gatttttaac agcgatgcca
    23881 gctattttat tttcaagcat tttcattttg attgctgcgg ttcctgagat ttttggttac
    23941 caatggcctg aatctgtctc cacatggcta tggaaagtct ataattattc gatgggtgtt
    24001 gtagggttac tcgtttcagc aaccactgct cgctgcttgg ctgattcgat gaatcgtcgc
    24061 ttaccgaaaa acaaagtaat caacaccacc tctgtgatgc tggcctctgt ggtaagtttc
    24121 ctgttgttaa gcgtctctcc gattgaaggt ggattttcaa cggaatatat ggggacaaag
    24181 ggactactgg cttcctttgt cgctgcattt ttaacagtta atttatataa gttttgtgtg
    24241 ctgaaagata ttaccattaa aatgcctaaa gaagttcctg gaacgatttc gcaaaccttc
    24301 cgagatattt ttcctttctc tttttcagta tttgcggccg ttttaattga tttaggcatt
    24361 cgacaaatct tccaaatctc atttgcagaa gcaatcatta cggtgctgca gccgctattt
    24421 acagcagctg acgggtactt aggaatcgct attatctggg gagcgatggc gctgttttgg
    24481 tttgtcggtg ttcatggacc gtcgatcgtg gagccggcaa ttgcggcaat tatctatgcc
    24541 aatgtggaaa ccaatctgca attgttcaat tccggtgaac aagcttctaa agtactgacg
    24601 gtgggtttag ggaattttgt aggaactatg ggaggaactg gggcaacatt ggttgttcct
    24661 tttatcttcc tactctttgc taagtctaag cagttgaagg cagttgggaa agcttccttt
    24721 attccagtaa gtttcgctgt caacgaacca ttgctatttg cggcacccat gattttgaac
    24781 ccttattttt ttattccatt tttgttaaca cctgttgtaa acgtttgcct gttcaagttt
    24841 tttgtcgatg tattgggcat gaacagcttt atgtacgttt tgccttgggc aactccagca
    24901 ccgatcggtt tggttctagg aacgggcatg ggtatcttag cttttgttct agcagcggtt
    24961 ctaatcgtcg ttgacttcct gatctatcta ccattttgca aagcatacga tgctgtctta
    25021 gtagagaaag aagcacaaaa agcagaggtg atcgcaaccg aggaaggtgc ttctgccgat
    25081 gtacgtgagg aagtacctgt ggcagcgagt caagttatca gcgatgcaaa taaagaaatc
    25141 aatgtgttgg tactttgtgc tggcgcaggg acaagtgcga tgttagctaa tgctttaaaa
    25201 aaaggtgcat ctgaatcgaa agcaccgatc agtgcaatgg cgggtgctta tggttctcat
    25261 tacgaaatgc tgcaggattt tgatatggtg gtcttagcac cgcaagtcaa ttcttactat
    25321 gatgatatca aagaagacac cgatcgctta ggaatcaagc tggttgcgac aaaaggagca
    25381 gaatatatcg gattaacgag agatcctgaa aaagctgttc agtttattct gaaccaattt
    25441 caataaagga ggagccataa tgaaaatggg aatttggttg cgtaaagctg ttttatttgc
    25501 agctgtgtta ggggctttgg gggctgtttc aaaaactgtt gaagcatctg ccagcgaatt
    25561 tattcgcgga gcagatattt cgatcctgtc agatatggaa aaatctggtg caaaatatta
    25621 tgaagacgat atttcaaaag atgccttaga gattttaaaa aataatggtg tcaactatgt
    25681 tcgactgcgt ttgtggcagg atccttataa tagtaatgga gaaagttatg gagcagggac
    25741 aaatgatctg gaaacgacga ttgaccttgc aaaacgagca aaaaatctgg gacttaaggt
    25801 gctgctggat tttcactaca gtgatttttg ggttgatcct gggaaacaga atcttcctaa
    25861 agcatggcaa ggtctgacct ttgaagaaat gaatacggca ttgtacgact atacaaagaa
    25921 cgttctgtcg gaaatgaagc agctggacgt ttatcctgat atggttcaga tcggcaatga
    25981 actgaatagc ggaatgctct ggccttatgg caagtcttgg ggagaaggcg ggggagaatt
    26041 tgatcgattg gctgcttttt tgaagtctgg tatccaagct gttcgagaca ctcaacctaa
    26101 aacaactcca gtaatgctcc acttagctga tggtggcgat acaggagcat ttacttggtg
    26161 gtttgatgaa atcacgagtc gaggtgtgtc atttgattta attggagtgt cgtattatcc
    26221 gtattggcac ggttcaatga atgaacttca aaccaatatg aatgcgatca gccaacggta
    26281 tggtaaagaa gtgatcgtag ttgaaacagc ttatgggcat acaacagcga atgccgatac
    26341 tatgccaaat gcttttggtg aagccgaagc agctgcgggg ggatatgaac caacaccgac
    26401 aggacaagca gagtatttgc tggatcttgc cgatcggatt caggcagtac cgaataatcg
    26461 cggggcgggc ttcttctatt gggaacctct ttggtacaac ggaaatgtga gttgggcaac
    26521 acaagcaggt atgaattatc tcagtgttca atccactatg ggaaacgaat gggacaacca
    26581 agcgatgttt gattttgctg gaaacgcctt gccaagcttg cgtgcgttta aacaagcagg
    26641 ggcgcaaata aatctagtta aaaatgcaag tttcgaaagc gatggaccaa cgaccagtcc
    26701 ttccaattgg caaacctggt ttcaacaagg aaccgctaag ggtacgatca aaacggaagc
    26761 gggcactttt tttggggact acaaactaac tttttggcag gatcaagcat acgaggcatc
    26821 agcttatcaa aatgttagtg gcataagcaa aggaacttac acattgagtg cctatgtaat
    26881 gtctggggga gagtttgata ctaatcagtt atatatcaaa aattatggtg gagcggaact
    26941 aaatactgcg gttgttaaaa caagtgaatg gacaaaaatt acaatcaaag acattccaat
    27001 tacaacaggt gtttgtgaaa ttggggttta tagcaaggct tcaggaaatg cttggacgaa
    27061 cgttgatttt gtttcattgg tgaaacaata gactgcggat cgtttcgatt caagatcgga
    27121 ggttcccttc agccacatcg ttagcgtaga aggcaggaat cgaacctgca cctcgccttt
    27181 gccaaaaata accagaaacg atttccagtc atttttacca aaacgagtaa agcaccagac
    27241 ttcatctaca ctttgtatta tgctaaagag agttcaacga tttcgtaaga agcaatgaaa
    27301 aaattgcaaa aagttgtgca gataacataa agtttgttta agaaagggta accttagaat
    27361 tccgtcgtga atatgatttg agcacttaga tggatagaaa aaagttctcg gaaaacagtt
    27421 cgtttttcga gaacttcttt ttatgataag aatgaaacaa attaacttat attatagtct
    27481 cttttggtag ggaaccatct tatttttaat gaaaatcaag cattttagct attgaagaat
    27541 gttgatttat agggattccc cctccatatt atgttggctc tttattccca ctccactgtt
    27601 gctggtggtt tagaagtgat gtcatacacg atacggttga cgtgggctac ttcgtttacg
    27661 atacgtacag agattttttg caagacttcc catgggatac gggcaaagtc agcggtcatt
    27721 ccgtcgattg aagttacagc acggataccg actgtgtagt cgtaagtacg tccgtcaccc
    27781 ataacaccga cagagcggat ccctggaaga acagtgaaat attgccagat atcacggtca
    27841 agtccagcag ctgcgatttc ttcacgcaag atagcatcag attcgcgtac gatttctagc
    27901 ttgtcttcag tgatttcgcc aagaacacga ataccaagac caggaccagg gaatggctga
    27961 cgccaaacga ttgaatcagg catgcctaac tgagtcccta attcacggac ttcgtctttg
    28021 aataatgtgt ttaatggttc gatcaattca aattgcatat cttctggcag tccacctaca
    28081 ttatggtgag atttgatcgt ctgtgctgtt tctgtaccag attcgataac gtcagtatac
    28141 aacgttcctt gtgctaggaa agagacgcct tcttcaccag caagttttgt tgcttcgtca
    28201 tcaaaaacat atacgaattc attcccaatg attttacgtt tttgttctgg gtcagataca
    28261 cctttcaact tgtctaagaa gcgatctctt gcattgactt tgatgatgtt caagccgaat
    28321 tttccgccta gactttccat tacttgttcc gcttcgcctt tgcgtagcag accgtggtca
    28381 acaaagatag atgtcaattg gtcaccgatc gctttttgta aaagaacccc aacgacacta
    28441 gaatcaacgc cacctgaaag accaagcaag acttttttgt caccaacttg ttcacggatt
    28501 ttttctactt gcatatcgat aaagttatcc attgtccagt taccttcaca gtgacaaaca
    28561 tcaaaggcaa agtggcgcag caattcgatt ccgtattcgg aatgacgtac ttctgcatgg
    28621 aattggatac cataaaaatt acgtgttgtg tcttggatag aagcaatcgg acaatctttg
    28681 cttgttccga ctgtttcaaa gccttcagga actttagtta ccaagtcacc atggctcatc
    28741 catactgttt gttgttttgg tgtttcttta aacaagacag cttgatcgct tgttacttct
    28801 aacaaggctt tcccatattc gcggttttta gcaggctcta cggaaccgcc aagtttgtac
    28861 gtgatcaatt gcataccata gcaaatccct aatacaggaa tacctaaatt gaagatctca
    28921 ggatcgatcc caaatgcatt ctcatcatat acactgtttg gtccgccaga aagaatgatt
    28981 ccctttggat tcatcgcttt tacttcttct gcagtaatgc ggtggctcaa taattcggaa
    29041 aagactccaa gatcacgaat acgtcttgtg atcaattggt tatactgact accgtagtct
    29101 aatacgataa tcttttcaac gttcgtcata tcggcaacgt ttgtcaaatt tattacccct
    29161 atccctttaa attttatctc aaaatagtca aactatttta aaatgctaat attttcgcat
    29221 gaaaatcgaa tcgatgatat gattttcagt tttatggaga attatatcgg ctcttcctcg
    29281 tgtaggcaag atatattcct gtaaattttt caaattcaca tttttccata catcttttgc
    29341 cattttaaag gcttcttcac gatctccgat agcaaaacga taataataat tctctggatt
    29401 ttggaaagcc gtatccagta acgcaccaaa acgatccaaa taccattttt cgatcagcac
    29461 gggatcggca tcgacataga cagaaaaatc aaaaaagtcg ctgacgtaga tttgctgatt
    29521 cgcaggtaat tgaagcgtat tgatcccctc aacgatcaag atatcaggct gctggatcaa
    29581 ttcgtacttg tctggaatga tatcatacac atcatgtgag taaacaggta cttttatctc
    29641 ttcctgacca gattttacac ggttcaagaa atcgatcagc atctccatat cataactttc
    29701 aggaaatcct ttacggtcca tgattccacg ctcttttaat atacgatttg gataaagaaa
    29761 tccgtcagtc gtgatcagct gaacgtttct tcgtttgaag gtacgagaca aaatcgtctg
    29821 cagcagtctg gctgttgtac tttttccaac ggctacactt ccggcgatcc cgataataaa
    29881 aggcggtact tctacgtatt tatgaagaaa taaaccttta ctcaagaaca aagattcaaa
    29941 ttctttcata taaagataaa tcagatgagc aagaggtaca tagatatctc gaacatcctg
    30001 catggaaatg cggtcattca aacttttgat attatctagt tcatcttgtg tcaaaggtgc
    30061 ttgcccatta cgataaaatt ctcgccattc ttctcttgcc acacggtagt agttcattgg
    30121 atcattcata ttagcctcct gcgttctccc atcaatggaa ctattttaac acaaaataaa
    30181 cgcaaacccc accacttaag agagattgcc gatctttttg tataatcttt ataatagaaa
    30241 gaaacggagt cttattcttg acttgcgcac ggatacataa aaggatagac tgttgtcatg
    30301 aaagaaatga gaaggatgga agacatgaca aatcattact attctgaacg cccagatacg
    30361 gcgcacgatt ttgaacaatg ggcttttgag ttaaaaggca gaacctttca atttgtaaca
    30421 gattctggtg tattttcacg tgaaacagtg gattatggtt cacgtgtcct gattgatgct
    30481 ttcgattggg aagagttacc tgatgaagga aagatcttag atgtgggatg cgggtatgga
    30541 ccgattgggt tagctttggc atttgcgaca cagcggtttg ttgaaatggt cgacattaat
    30601 gaacgagcgg taagtctagc gcaaggaaac gcaaagcgta accaaatcga acatgttgat
    30661 atccatcaat cgaatatcta tgaagcagtt catgaagaaa cctatgcagc tatcgtaagc
    30721 aatccgccga tccgtgctgg aaaaaaagtt gtacatgaaa ttttgacagg cgcatatcct
    30781 cggttgaaga agggcggtac actgacaatt gtgatccaga agaaacaagg agcgccgagt
    30841 gcgcaaaaga aaatggaaga aacatttggt aatgcagaaa tcgtcacaaa agataaagga
    30901 tattatatca tcaaaagtgt gaaagaataa ctagctgaaa aatagtggag cgacgaaaga
    30961 gtggaactgg gcgtgcatgt acgggtacgt atcagttctt tttttgttga gaagttttta
    31021 aaaacaaaga actatttttt cttcaaaaag aaatagtagg aacagagtcg ttgtttttta
    31081 cgataaaata aataagatac agaacttgta tagaaggctg ccaagtcggt agataaagga
    31141 ggtttttatc aaaaaacata ctttcctttt ttctctattc ttttcttggg ggttttggta
    31201 cactgatagg gaatttagat agaagggagc caataggatg aagctgacca tcagagatat
    31261 cgcagaaatg gcaggcgtat cagtaacaac tgtctcacag attttgaata acaaaggaag
    31321 cagatttagt gataagacaa gaaaaaaagt gttgtcgatc gtgaatgaac atcattataa
    31381 gcccgattat ttcgcttcaa atgtgatcaa tcgacattca aagaccatcg gaatgatcgt
    31441 ccctgatgtc actgatttct tcttctccaa actgatcgaa ggtgtagaga gctatttgaa
    31501 tcctttgggc tatgtgatca tgttatgcaa ttctcgtcat agccaagaaa atgagatcaa
    31561 gtatttacaa gagctttccc accgctcagt ggacggaatc ttgctggcca caccaaatat
    31621 tttacctgag aaatatgcat tagacagcgg tttttatcaa aaaatgccaa tcattttaat
    31681 cgatcgcgga ttgaatcgtc gggataacgg acggcttatc gtaaaggaat atgagggcgc
    31741 ctatcaagct gtttcgctgt ttattgaaaa tggccatacg aagatcggca tgctgaagga
    31801 aaccactgga tactatcaat tagaagaacg tttcaacgga tatcgtcatg cgctgaaaga
    31861 tcatggcctg ccttttaatg ggaaatttgt tgaacatgga gagttgactg tccaaggtgg
    31921 atatactgca tcaaaaaaat tactcaaaca taaagatatc acggctattt tttgcgggaa
    31981 tgatgccatg gcgatcggat gctatcaggc gatcgatgaa ttgggcaaaa aaatcccaga
    32041 agatatctca gtcataggat tcgatggatt aaagctgtca gaatacatga ttccgcaatt
    32101 gacgacagtg cagcagccaa gttttgatat tggattttat gctgcacgtt tcctgatcga
    32161 tacgatcgaa tttcctcaaa gaaaagttcc gaacaaagtg ttcgaaacaa aattaattat
    32221 tcgtgagagc gtaaaagtat tgacaaaggg ctaactcggc ggtatattgc ttatgtaagc
    32281 atttacaaag gctagttagt gcttttttta tttttagtaa accagtttac taaagtggtt
    32341 tacctttcct aaaaataatg tataataccg aatgaagaca aggaggatca ggcaatgcga
    32401 atggtggatc tgattgaaaa aaagagggat ggaaacgagc tgtcgaaaga ggaaatcgag
    32461 tacatcgtca cgaactatac gaatggaaag attccagatt atcaagtcag tgcattattg
    32521 atggcaattt tttatcaaga catgacaaat gaagaaatca cgaacttaac attggcgatt
    32581 gctaattctg gtgatgtcat cgatttgagt tctcttgaag gaataaaggt agataagcat
    32641 tcgacaggtg gcgttggtga cacgaccaca ttgattttag cccccctagt agctagtgtc
    32701 ggagtgacag tggctaaaat gtctggacgc ggtctaggtt acacaggtgg tacactagac
    32761 aagctagaag caattcccgg atttcagatc gagctttcgg atgaagaatt tgtacgcatt
    32821 gttaatgaaa gtaaagtagc ggtaattggt caatcaggaa acttggcacc ggcggacaaa
    32881 aaattgtatg ccttgcgtga cgtcacagcg accgttgatt ctcttccgct gattgccagt
    32941 tcgattatga gtaaaaaaat cgcggcagga gcagatgcaa tcgttttgga tgtgaccact
    33001 ggagacggtg ctttcatgaa gaacatcgaa gatgcaagac gtctggcaaa gaccatgacg
    33061 agcattggga aactggctaa ccgagaaaca gtggcggtta tctcagatat gtcagaacct
    33121 cttggcgaag ctatcggaaa tagtctagaa gttgtcgagg caatcgaaac attacaagga
    33181 aatggtccag aagatctagt agaaatgtgc tacgccttag gaagccaaat ggttgttctt
    33241 gctggcaaag ctaagacaac cgatgaagca agaacgttgc ttcaagaagc tctggaatca
    33301 ggtaaagcgt tagcaaaatt caaagaaatg atccaaaatc aagggggaga cccagcgatc
    33361 gtggaacatc cagaacgaat tttgactgca cgctacacaa tggagcttcc tgctaaacaa
    33421 agcggagttg tgtcaaaaat cgtggcaaat gaattaggaa ttgctgcaat gatgctaggt
    33481 gctgggcgta aaaccaaaga agaagatatt gatcacgctg ttggattgaa actgcataaa
    33541 aaaattggtg acactgtgac aaaaggtgag tcactattga cgatctatag taacgacgaa
    33601 gaaatcactt ctgtcattga attattatat aaaaatattg aaattggtga atcggcaatg
    33661 aagccaacgt tgattcatga catcatcaca gaataaagga aaaaagcgga ctttaccgtt
    33721 ttaaagaaaa ggggaaaaga aatggaacta aaccgaatga tcgaccatac tattttgaaa
    33781 gcagacgcat ctaaagaagc tgtgatgcaa attatcgaag aagcaaaaaa ataccatttt
    33841 tattctgtct gcatcaatcc agcatgggtt gcgctagcga aagaacaatt acaaggtgag
    33901 ccagtagccg tatgtacagt aatcgggttt cctctaggag caaacacacc cgagacaaaa
    33961 gcatttgaaa caacagatgc catcaataac ggtgcagatg aagtcgatat ggtcatcaat
    34021 atcggtgctt tgaaatcaaa ggatgatcaa caagtccaaa aagatatcga agcagttgtt
    34081 gaagcagcaa aagatcgtgc gcttgtcaaa gttattattg aaacatcgtt attagataga
    34141 gaagaaatca tccgagcttg cgaaatagca aaagctgcag gcgctgattt tgtcaaaaca
    34201 tcaactggtt tctcaacagg cggagcaaag gtagaggatg tccgtttgat gcgtgaaact
    34261 gtaggaccag aaatgggtgt caaagcttcc ggcggtatcc ataacgaaga agaagcaatg
    34321 gcaatgatcg aagctggtgc tacaagaatc ggaacaagcg caggagtagc aatcgtatct
    34381 ggttcaactg gtgaaggcta ttaatttttt caaaggagaa gctcatggca gaactcaaac
    34441 aagaatggat caacacagct attgaagcgt tagataaagc ctatgttcct tattcccatt
    34501 ttccggtagg cgcttgtctc gtaacggaat caggcaagat ttatcaaggc atcaacattg
    34561 agaatgcatc atttggatta acgaattgtg ccgagcgcac agcttttttc aaagcagtat
    34621 ctgaaggaga aaaaacattt acgcatctag tcgtagcggg gcacaccccc gatcctatct
    34681 ctccttgtgg tgcatgtcga caagtaatgg cagaattctg ttcccctgat atgccggtta
    34741 cgctagtagg agacaatggt gtgacgaagg caacgaccgt tcgtgcactg ctgccttatg
    34801 catttactga aaaagatcta taactcatgt tcattcagtc agctgactgt tgaaaaataa
    34861 aacaattatt ttaggaggct ttacagaaat gaaaaaagca aaattatttg gtttaggtgc
    34921 tgtcgcacta gcagcagggt tatttttagg tgcatgcggc aacaacggct ctactgattc
    34981 atccggtggc aaaagctcat cagacacaac aactgcagca ttgatcacag acacaggtgg
    35041 tgtagacgac cgttcattca accagtcagc ttgggaagga ctagaaaaat gggggaaaga
    35101 tcaaggactt tcacgaggaa atgatggctt ccaatatttc caatcttcaa atgaatcaga
    35161 ttacatccca aatattgacc aagcattgaa tgctggattc aaaacgattt ttggtattgg
    35221 ttataaacta aaaccagcga tccaagaaca agcaacaaac aatacaggta cgaactttgt
    35281 tattatcgat gatgtcatcg atggattaga taacgttgta tctgcaactt tcaaagacaa
    35341 cgaagcttct tatcttgcag gtgttgcagc agcttatacg actgaaacaa acgtagtcgg
    35401 ctttatcggc ggtgtaaaag gtgaagtaat cgatcgtttt gatgctggat tcaaagccgg
    35461 tgttgatgca ggtgcgaaag aattaggaaa agaaatcaaa gtattgaatc aatatgctgg
    35521 tgacttctct gctccagata aaggacgttc aattgctcaa ggtatgtatg cacaaaacgc
    35581 agatatcatc ttccatgctt caggcggtac aggtaacggt gtattccaag aagcaaaatc
    35641 attgaacgaa tcaggcgaca aaaaagtttg ggttatcggt gttgaccgcg accaatcaga
    35701 cgaaggcgaa tacacattaa acggcgaaaa gaaaaacttc acattgacat ctacactgaa
    35761 agcagttgga acagtagtag aagatttggc acaaaaatca gcggacggta aattccctgg
    35821 cggcgaacac actgtttatg gattgaaaga agacggtgtt ggtctaacag aaggacaact
    35881 ttctgacgaa gcgaaaaaag cagttgatga agcaaaagaa aaaatcattt caggtgacgt
    35941 aaaagttcca gaaactcctg aagaaaacta aaaccatttc tatagccttg ttagacacat
    36001 gtttgacaag gctatggttt gattagaaca agaattgttc atttatccaa gctgccttgt
    36061 gacagtttgg ataagggaac aactccctga agtaatgttt gtttcaaaag aagaacgctc
    36121 actaagcgtg aatcttagtc aatcgttgcc ctagatagaa atcgttagga tgtgaaaaaa
    36181 aggtggttca agaaaatatt gtcatagaaa tgcagaatat taccaaagaa tttggaacat
    36241 tcaaggcaaa tgacaacatc aatttacaag taaaggcagg agaaattcat gcgttgcttg
    36301 gagaaaacgg tgctggcaaa tctacattga tgaacgtgct ttccggatta ttagagccga
    36361 catcagggaa aattttgatg cgtgggaaag aagtaaagat cacaagtccg accaaagcca
    36421 accaattagg gattgggatg gtccatcagc actttatgct tgttgatgcc tttactgtaa
    36481 cagaaaacat cgtgttggga agcgagccta gtcgcgcagg gatgcttgac cataaaaaag
    36541 cacgaaaaga gatccaaaaa gtttctgaac agtatggatt gtcagtcaac ccagatgctt
    36601 acgttcgtga tatttcagtt ggaatggaac aacgggtaga aattttaaaa acactttacc
    36661 gaggagcaga tgtactgatt tttgatgagc cgacagctgt attgacccct caagaaattg
    36721 atgaattaat cgtgatcatg aaggaattag tcaaagaagg caagtcgatc attttgatta
    36781 cgcataagtt agatgaaatc aaagcagtag ctgaccgctg tacagttatc cgccgtggaa
    36841 aaggaatcgg tacagtcaac gttaaagacg taacctcaca acaattagcc gatatgatgg
    36901 ttggaagaac ggtttcattc aaaacgatga aaaaagaagc gaagcctcaa gaagtcgttt
    36961 tgtctattga aaatctagtg gtgaaagaaa atcgtggatt agaagccgtg aaaaacttga
    37021 acttagaggt tcgtgctggc gaagtacttg gtatcgctgg aatcgatgga aacgggcagt
    37081 cggagttgat ccaagctttg actggtttgc gaaaggcaga aagcggacat atcaagctaa
    37141 aaggggaaga catcactaat aaaaaacccc gaaaaatcac tgaacatggt gtaggacacg
    37201 tgccagaaga ccgtcataaa tacgggttag tcctagatat gacattatct gaaaatatcg
    37261 cactacaaac ctatcatcaa aaaccttaca gtaaaaacgg catgctgaat tattcagtaa
    37321 tgaatgaaca tgccagagaa ttgatcgaag aatatgatgt tcgaacaacg aatgaacttg
    37381 ttcccgcaaa agctttatca ggcggaaatc agcaaaaagc aattatcgct cgtgaagtcg
    37441 accgagatcc tgatctgttg attgttgcaa atccaactcg tggactggat gtaggtgcga
    37501 tcgaatttat tcataaacgt ctgatcgaac aaagggacaa atacaaagca gtgttactga
    37561 tcagtttcga attagaagaa attttaaatg tttcggatcg tattgctgtt atccatgaag
    37621 gagaaattgt cggtatcgtt gatccgaaag aaacatctga aaatgaatta ggtctattga
    37681 tggcgggcta taccttagaa gaagcacgaa aagagttaga aaagggggct ggcgcacatg
    37741 aatgatcgca gcgaaaaatt ccgtaattta ttagtgcctg tactttcagt tgttttagga
    37801 ttgatcttag gcgcgatatt gatggctgct tttggttttc agcccatcca aggttattct
    37861 tctatgctag atgcagccct tggttcgcaa agagggatag gtgaaacgtt aaggcaagca
    37921 acgccgttga tttttacagc attaggtttt tctgtagcta actcagctgg gtttttcaat
    37981 attgggcttt ctggacaagc actttgcggg tggatcgtca gtgtctgggt ggcacttgcg
    38041 tttccagatc tgccaaaagt tgttttgctt ccgttatgcg tgatcttagg tgccttagct
    38101 ggtgcgatgg cagcggctgt tccaggttta cttcgagcat tttttggaac aagtgaagtg
    38161 atcgtaacaa tcatgatgaa ctatatctta ttatacgtaa gcacatttgc tttacatgaa
    38221 gtgatgcctg aaagctatcg ctcaagtttg gactcttcca atatgatcag tcaaaatgcc
    38281 tcactgcggt taccatggtt aacacaagct tttggcggct ctcgcgtaaa ctcaggatta
    38341 tttttagcaa tcatcgcgct tattttcgtt tggctgctga tgaagaaaac gactttagga
    38401 tttgaaatcc gttctgttgg attgaatcct tttgcttctg agtatgcagg aatgagtagt
    38461 aaacgtacga tcgttttgtc gatggtcatt tctggcgctt tggcagggct tggcggtgtg
    38521 gttgaaggat taggaacgta tcaaaacttc tttgttcaaa cgacttcgct atctattgga
    38581 tttgacggaa tggctgtttc cttgcttggt tctggatcag caatcggtat tcttttatca
    38641 gcgatcttgt tcagtatttt gaagatcggt ggacttggca tgcagacagg agcaggtgtt
    38701 ccgtttgaga tcgtcaatgt atcgattgct ttgattatct tctttgtcgg gatcaacttc
    38761 ctgattcgtt tcgtcatggc taaagtcttc caagggaaaa aacaagaaga gatcgttgcg
    38821 acaatagaat cgaaaccaac gaatcaaaac catgaagggg gcgaactata atgtcgacta
    38881 tcgaattaat tgccttgatc cttacttcta cactggttta ttcaacaccg ctaattctaa
    38941 cgtcactagg cggaactttt tctgaacgaa gcgggatcgt aaacgtaggg ctagagggaa
    39001 tcatggtcat gggtgctttt agttccatcg ttttcaactt gacgatgagt ccatcacttg
    39061 gttctatgac accatggatc gctattttag tgggtggcgt cgtaggtatc ctattttctc
    39121 tgattcacgc agtagcaaca gtcagtttgc gtgctgacca tattatcagc gggacagtaa
    39181 ttaatctgat ggcacctgct ttaggtgttt tcttgatcaa agcatggtat ggtaaaggtc
    39241 aaactgataa catcacgcag aatctggggt atttttcttt cccaggatta gcagatattc
    39301 cggtcattgg accaattttc tttaaaaata cattcttacc tgcttattgt gctattttga
    39361 ttgctgtcct agcttgggtg atcatcttca agacgaaatt cggcttgcgc ctccgttcag
    39421 ttggtgaaaa tcctcaagct gccgacacat taggtatcaa tgtatatgct atgcgttatt
    39481 caggcgtact gatttctggc ttgttaggcg gtatgggcgg tgctgttttt gctcagtcga
    39541 tttccggtaa cttttcagct ggtacgatcg ttggacaagg gttcatctcc atggctgcta
    39601 tgatctttgg taagtggaat cctttagggg caatgggtgc ctcattgttc tttggttttg
    39661 ctcaaagctt gagtatcatt ggcgcacaat taccagttat ttcttcgatt ccgccagtat
    39721 tgctgcaaat tgctccatat ttgctgacaa tcattgtgct ggttgttttc ttagggaaag
    39781 cttctggtcc aaaagcaaac gggaagaact atatcaaatc aaaataatct tttagcgaat
    39841 aaaaagggtg ggttacggga agtgtttttc gtctcgccct ttttgtttta aaggcaataa
    39901 aaataaaaag aaagaaggaa taatcatgtt taaacgcgta catctgatcg tcatggattc
    39961 agtaggaatt ggagaagcac cagatgccga taagttcggt gatgttggtt ctgatacatt
    40021 aggacacatt gcgaaagaag caggattgaa cgttccacat ttagagcagc ttggtttagg
    40081 aacgatccgt cctcttgaag gtgtggaatc agtgaaagat catcaaggct atgcgacgaa
    40141 attagaagaa gtttctgtag ggaaagatac gatgacaggc cactgggaaa tcatgggatt
    40201 gaatatcaaa actccatttc gtgtgtttcc taatggattc cctgatgatc tgatcaaaca
    40261 aatcgaagaa ttttctggcc gtaaagtcgt atgtaacaaa ccttatagcg gaacagctgt
    40321 tattgacgat tacggtgaac accaaatgaa aacaggcgat ttaattgtat atacttctgc
    40381 cgatcctgtc cttcagatcg cggcacatga agaaatcatt cctttagaag aattatatcg
    40441 gatttgccaa ttcgttcgtg atataacaaa agatgatccg tatatgattg gccgaatcat
    40501 tgcacgtccg tacgtgggtg aaccaggaga cttcacacga acaagcaatc gccatgatta
    40561 tgcgttgaat ccattcggac acacagtcct tgattctttg aaagaagcag gaaaggacgt
    40621 gattgctgtt ggaaagatca acgatatttt caacggacaa ggaatcactg aatctgtccg
    40681 cacgaaaagc aatatggatg gcgttgacca gctgctgaaa gttatgaaaa acgatttttc
    40741 aggaatcagc tttaccaatt tagtagattt tgatgcatta tacggtcatc gtcgggatgt
    40801 agtcggctat gcgcatgcga ttgaagaatt tgacttacga attccagaat tgcttgaagc
    40861 gatggaagaa gacgacttgc taatgattac agccgatcac ggaaacgatc cgacgtttac
    40921 tggtaccgat catacgcgag aatatgtacc gttattggtt tacagtaaaa aaatgaaaga
    40981 acaaggggca ttgcctcaag gatattattc agatatttca gcaacgattg ctgagaactt
    41041 tagtgtttct tcgactgaaa atggcgaaag cttcttgaaa ttattaaaat aatcttttta
    41101 aataggagaa agcaaatgac caagttaagc gagatgttac aagaaacgac ccagtttatc
    41161 cttgataaag gagtaaaaga aattgatttt ggtttgattc taggttcagg attaggtgaa
    41221 ttagcagatg aaatcgaaga tgctgtaatg atttcttatg atgagatccc gttctttcct
    41281 gtttcgacag tagttgggca tgcaggaaag ctagtttatg ggtctctggc aggcaagaaa
    41341 gtgttagcca tgcaagggag attccactac tatgaaggtc attccatgca aacagtgacc
    41401 tatccagtac gtgtgatggc cggactaggg gcccattcag tcattgtgac taatgcatgt
    41461 ggtggtgtca acgaatcatt tgtaccagga gatttgatgc tgattaccga tcatatcaat
    41521 tttactggtg aaaatccttt gatcggtccg aatgaagatg caatgggccc tagatttcca
    41581 gatatgagtc aagcttatac gcctgactat caagcaacag caaaagcagc agcagaaaaa
    41641 ctaggtttgg atcttaaaga aggcgtatat atgggctatt ctggaccaac ttatgaaacg
    41701 cctgcggaga tccgaatgtc tcggacaata ggagccgatg cagtagggat gtcgactgtt
    41761 ccagaagtca tcgtagcagc tcacagcgga ttaaaagtgt taggaatctc ttgtattacc
    41821 aacttagcag caggtatgca agcgaatctg agccatgaag aagttgtaga aacaactcag
    41881 agagtcaaac aatcttttaa agcattggtt aaagaaacat tgacattatt gtaataggag
    41941 gaacaataaa atgagtgtgc atattgaagc gaaagaaggc gagatcgccg ataagatttt
    42001 acttccggga gatcctttgc gagctaaata tattgcggag acttttttgg aagatccagt
    42061 ttgttacaat caggtacgcg gtatgttagg ctttaccggc aaatataaag gagaacgtat
    42121 ttctgttcaa ggaacaggaa tgggaatgcc atcagccacc atctatgccc atgaattgat
    42181 ccagtcgtat ggtgtaaaga aattgattcg agtaggtaca tgcggtgcat tgtcaaaaga
    42241 cgtccatgtt cgtgatttag tactagcaca aggtgcagcg accagttctt cgatgatcga
    42301 aaaaaatttc caagcctttc attttcctcc aatcagtgat ttcaatctat tactgaaagc
    42361 atatgagata gctaaagaaa agggctatac ggttcatgta ggaaatgttc tttcggaaga
    42421 ttctttctac aaagatgatt tgacagagac gttccaatta gctgaacttg gcgttttagg
    42481 agtggaaatg gaagcagcgg ctctttatta cttaggcgcg aaataccatg tgcagacatt
    42541 aagtttgatg accgttagcg accatttgat caccggtgaa gaaacaacag ctgcagaacg
    42601 tcaatctacg ttcaatgaaa tgatcgaagt aggattggaa acagcgatag cagaataaga
    42661 aggaaatgaa aaagctgccc tgcattaagt aataagaaca tccaagcaaa aatagctcct
    42721 catgtttttg acttggctgg acttattatt gcaaatgtaa gcttttgaac accgtttacc
    42781 aagaggttgt gaatctgccg tttaactctg agtcacaagg aacaatttca aaaaacagct
    42841 tttcatgttt gatgaatatt gtgtcttgtg accgagaagt tggcaggaga aaaccgttta
    42901 tacgaaaaaa gggactgtga caaatcgtgt cacagtccct tttttatagt gttttttata
    42961 ggtagtattt tttagcaact gttaagtcat catttaattc ataaacaagt ggttgacctg
    43021 ttggaatttc aagatccatg atgtcttcgt cagagatacc ttcgatatgt ttagctaggg
    43081 cgcgtaatga gttaccatgt gcagctacta aaacagtttt gttgtctagt aatgctggag
    43141 caatttcatc ttgccagaat ggcaaagcgc gttctaatgt cactttcaag ttttctccac
    43201 caggaatatc gcgtttgtct aacattgcgt aacgacggtc atttgctgca gaaccttcgt
    43261 cagtttcttc cattaatgga ggaagagtat cgtaagaacg acgccagatg tgcacttgtt
    43321 cgtcgccgta tttttcagct gtttcttttt tgttcaaacc ttgtaattta ccatagtgac
    43381 gttcgtttaa gcgccatgat ttgatttgtg gtacccaaag ttgatctgaa tattctaaga
    43441 tcaagttaca tgttttgatt gcacgagtta aaacagaagt gtaagcaaca tcaaattcga
    43501 ttccagcttc tttgattttg cgtccgcctt cttttgcttc ttcgattcct tctggtgcta
    43561 gatttacatc agcccaacca gtaaattggt tcaatgcatt ccattcacta agaccgtgac
    43621 gagaaaaaac taattttggc atgtttagct ctccttttaa gataggttta tatttctgaa
    43681 attttcagtt ctgttttatt gtacaatttt ttggaagaga tttccatcga aaaattagat
    43741 aagattctat gagaaaacaa gtagagaata taaaaaaacg aacagtgccc acgcaactgt
    43801 tcgtttttag gtattagatg cataattgct cgatttcttt ttcaggtaaa ttgatatatt
    43861 tataacatgt tccaactgaa acaccgcatt ttgaagaaat aaattggatc gtttcttttt
    43921 tttcataata gagacgtcgg atttttttga tcgtttttgc atcgattttt ggtcttccgc
    43981 cgatttttcc ttttttacga gcttcatcta atccaactaa tgtacgttct ttgatcaatg
    44041 cgcactccat gtttgctagg ttttccatta attggaaata gatttttccc atttcgcgcg
    44101 tatcgattcc ttcatcaaga ctaatcaagt gcagcccgcg atctttgaat aattgcgtga
    44161 gttctgttaa ttgccgcgta gatttgccta atcgatgaag ttggcaaatg actagtgcat
    44221 cgccttgttg catgtcagcg atcactgact ccaaatgaac agcagtagaa tcattttgtt
    44281 ccacttcata tgtctcatgg aaaatttctt gacaaccaaa gtcagataat tgatcaacct
    44341 gagttgttaa atcaacgtca tttatggttg tgcgtgcata accaataatt ttcatggtga
    44401 ttcctttcta gcagtgattt tctaactgga tataacagta aaaatgtagc ataaagtacg
    44461 tcaaaaagat agttttttcg caaattttcc gctgtctttt tttattggat gatcaagcag
    44521 tttttcttta tttacgacgg attgcgtcaa gaatctcaac gcctttttct gagccgacaa
    44581 ctactttaga aaccatgttt aggaaaagcc catgttctac taccccaaca agttcatcta
    44641 aataggtagc taagctttct ggctgttcga tgacttctag atgcagatca atgatgtaat
    44701 gaccaccatc tgtggttaag acttctccgt tttcgtctgt acgcaagacg ggttggaatc
    44761 ttttttcttc aaaaatatga agtagctgtt gggaaccata agggatcact tcaactggga
    44821 gtgggaactt cccaagttta tcgactaatt tagaagaatc aacgatccaa atcgtttctt
    44881 ttgagtacgt agcgactatt ttctcgaaaa gcaaagcagc gccgccgcct ttgatccctt
    44941 gaaaatcagc actgatttca tcagcaccgt ctattgtcaa atcgactacg gggacttcat
    45001 caatactctt taacggtatt cctaattttt ccgcttgttc ttttgtcgca tttgaagtag
    45061 taactcctgt gatacgtaaa ccttcttcac gcatacggcg accaatctct tctaccatat
    45121 aataggcggt tgaaccggtc ccgagaccta cgatcatccc gtcctttacg aatttcgcgg
    45181 cttcaatgcc agctaattgt tttaaattca tctttgcgtc cacctttttt tagtttgagc
    45241 atcgtgcttt cctcactatt ttagcgtgat ttacggtaaa aagataccat tcttttttat
    45301 tccaataaag ctttttcaaa aaaaattgca tctcttgttg acatgttttt tcattcgtga
    45361 tattctaaca aaggtgcttt atctacaggt ttctgtgaag aaacgtgctg actgtttatc
    45421 aaagcatatt tgataaggat gcagacattg catttatttt ttatcacaaa aaaagaacca
    45481 cctggatgtg tggaactaga cgcaaatcgt gggaaggagg aaacaaggaa tgcctacaat
    45541 taaccaatta gtacgtaaac ctcgtaaatc aaaggtggaa aaatctaatt cacctgcttt
    45601 gaacaaagga tataacagct ttaagaaaac tcaaactaac gtaaactcac ctcaaaaacg
    45661 tggggtagca actcgtgtgg gtacaatgac acctaaaaaa ccgaactcag ctttacgtaa
    45721 atatgcacgt gttcgtttgt caaacttaat tgaagttact gcttatatcc cagggattgg
    45781 tcataactta caagaacaca gtgttgtatt attacgcggt ggtcgtgtaa aagacttacc
    45841 aggggtacgt taccatatcg ttcgcggtgc tttagatact gctggtgtta acgaccgtaa
    45901 acaaagccgt tctaaatacg gaactaaaaa acctaaagct taattttaat tattaaacaa
    45961 atgaaacttg cttctactat atagaagtaa ttactatgaa caatatttcg gaaggaggag
    46021 ttacggatgc cacgtaaagg tcctgttgca aaacgtgatg ttttaccaga tcctatttat
    46081 aactcaaaat tagtaactcg cttgatcaac cgtgtaatgg ttgacggaaa acgcgggatt
    46141 gcagctaaca ttatctataa tgcctttgac ttgatcaaag aatctacagg taacgatcca
    46201 ttagaagtgt ttgaacaagc aatgaaaaac gttcaacctg ttcttgaagt taaagcacgt
    46261 cgtgttgggg gttcaaacta ccaagtacca gttgaagttc gccctgaacg ccgcgttact
    46321 ctaggtcttc gttgggtagt aaactacgct cgcttgcgcg gtgaacacac aatggaagaa
    46381 cgcttagcaa aagaaatcat ggatgctgca aacaacacag gtgcttctgt taaaaaacgt
    46441 gaagacactc ataaaatggc tgatgccaac cgtgcgtttg ctcactatcg ctggtaagat
    46501 tctctctgtt tggtcggctt ttataagtcg acctcagata gtgtccttat ttttagcaac
    46561 tagatagtaa agagaggaga aacaaggaga atgaccagag aattttcttt acaaaacact
    46621 cgtaatatcg gtatcatggc ccacatcgat gctggtaaaa cgactgctac tgaacgtatc
    46681 ttgtactata ctggtaaaat ccataaaatt ggtgaaactc atgaaggagc ttcacaaatg
    46741 gactggatgg aacaagaaca agaacgtggt attacgatca cttctgctgc tactacagca
    46801 caatggaagg gtcaccgaat caacatcatc gacacaccag gacacgtaga cttcacagtt
    46861 gaagttgaac gttcattgcg tgtacttgat ggtgctgtaa ctgttcttga tgcacaatca
    46921 ggtgttgaac ctcaaactga aacagtttgg cgtcaagcaa caacttatgg tgtacctcgt
    46981 atcgtatttg caaacaaaat ggacaagatc ggtgctgact tcttatattc agtatcaact
    47041 ttacatgacc gtttgcaagc aaacgctcac ccaatccaat taccaattgg tgcggaagac
    47101 gacttcacag gaattatcga ccttgtaaca atgaaggctg aaatgtatac aaacgatctt
    47161 ggaacagaga tcgaagaaac agaaattcct gaagaatacc gcgaattagc tgaagaatgg
    47221 cgcgaaaaat tggttgaagc tgtagctgaa actgatgaag aacttacttt gaaatactta
    47281 gaaggtgaag aaatcactga agctgagtta aaagaaggta tccgccgcgc aacagttaac
    47341 gtagaattct acccagtact atgtggttca gcatttaaga acaaaggggt tcaattacta
    47401 ttggacgctg ttcttgatta cttgccatca ccgttagata tcccagctat caaagggatt
    47461 gaccctaaaa ctgatgaaga agtagaacgt cctgcagacg acagcgctcc tttctcagct
    47521 ttagcattta aagttatgac tgacccattc gtaggtcgtt taactttctt ccgtgtgtac
    47581 tctggtgttt tacaatcagg ttcatacgta caaaacgcaa caaaaggcaa acgtgaacgt
    47641 gttggtcgta tcctacagat gcatgccaac tctcgttcag aaatctctga agtttacgct
    47701 ggagatatcg ctgctgctgt tggtttgaaa gacacaacta ctggggacac actatgtgat
    47761 gaaaagagtc tagttattct tgaatccatg gaattcccag aaccagttat ccaagtcgct
    47821 attgaaccta aatcaaaagc tgaccaagat aaaatgggtg tggctttaca aaaactatct
    47881 gaagaagatc ctactttccg tgctgaaaca aacgtggaaa caggcgaaac aatcatcgct
    47941 ggtatgggtg aacttcactt agatatcatc gttgaccgta tgcgtcgtga attcaaagtt
    48001 gaagctaacg taggtgcgcc tcaagtatct taccgtgaaa ctttccgtgc tggcactcaa
    48061 gccgaaggta agtttgtacg tcagtctggt ggtaaaggtc aatacggtca cgtatggatt
    48121 gaatttacac caaatgaaga aggtgctggt ttcgaatttg aaaatgcgat cgtcggtggg
    48181 gttgttcctc gtgaatacat tccagctgtt gaaactggtt tgaaagatgc tatggaaaat
    48241 ggtgtcttag ctggctatcc attagtagat atcaaagcta aactttacga tggttcatac
    48301 catgatgtcg attcaaatga aactgccttc cgtgttgctg cttctatggc attacgtgca
    48361 gcagctaaga aagctaatcc agtaatcctt gaaccaatca tggctgtaga agttgttatc
    48421 cctgaagatt atttaggtga tgttatgggt cacgtaactg ctcgtcgtgg acgtgttgaa
    48481 ggtatggaag ctcgcgctgg cggccaacaa gttgttcgtg cgatggttcc acttgctgaa
    48541 atgtttggtt atgctacaac tcttcgttct gctactcaag gtcgtggtac atttacaatg
    48601 acatttgacc actacgaaga tgtaccaaaa tcagtacaag aagaaatcat taagaaaaac
    48661 ggcggaaaag ctgaataatt tcttaaaacc ggatagtcct gcaaagagat tatccaaaga
    48721 gactatattt ttaatatagt ttcgtgtaaa ataattatcg atgatatggg catgtggtgt
    48781 accttgtgta catcatatac ctatcagaca aataaaaaat atattgtatt tttaggagga
    48841 cattttaaaa tggcaaaaga aaaatttgac cgttctaaac cacacgttaa cattggtact
    48901 atcggacacg ttgaccatgg taaaactaca ttaacagctg caatcacaac tgtactatct
    48961 aagaaaaacg gcggccaagc tatggcttac gatcaaatcg atggtgctcc agaagaacgc
    49021 gaacgcggaa tcacaatctc aacagctcac gttgagtatg aaacagatac acgccactac
    49081 gctcacgttg actgcccagg acacgcggat tacgttaaaa acatgatcac tggtgctgct
    49141 caaatggacg gagctatctt ggtagtttct gctgctgacg gcccaatgcc tcaaactcgt
    49201 gaacacatcc tattgtctcg tcaagttggt gttccttaca tcgttgtatt cttgaacaaa
    49261 gtagacatgg ttgatgacga agaattacta gaattagttg aaatggaagt tcgtgaccta
    49321 ttaacagaat acgaattccc tggtgacgat gttcctgtag ttgctggatc agctttgaaa
    49381 gctctagaag gcgacgcttc atacgaagaa aaaattcttg aattaatggc tgcagttgac
    49441 gaatacatcc caactccaga acgtgacaac gacaaaccat tcatgatgcc agttgaagac
    49501 gtgttctcaa ttactggacg tggtactgtt gctacaggtc gtgttgaacg tggacaagtt
    49561 cgcgttggtg acgaagttga agttgttggt attgctgaag aaacttcaaa aacaacagtt
    49621 actggtgttg aaatgttccg taaattgtta gactacgctg aagctggaga caacattggt
    49681 gctttactac gtggtgttgc acgtgaagac atccaacgtg gacaagtttt agctaaacca
    49741 ggtacaatca cacctcatac aaaattctct gcagaagtat acgtgttgac aaaagaagaa
    49801 ggtggacgtc atactccatt cttcactaac taccgtccac aattctactt ccgtacaact
    49861 gacgtaacag gtgttgttga attaccagaa ggaactgaaa tggttatgcc tggcgacaac
    49921 gtaacaatgg aagttgaatt aatccaccca atcgctatcg aaaacggtac taaattctca
    49981 atccgtgaag gcggacgtac agttggtgcc ggtgtcgtta cagaaatcaa agcttaattc
    50041 acttaagttt taaagtaaag caacatttat tcggacgtaa tcgctcggaa cgaaggtctt
    50101 aggatctttg ttccgagctt ttttatttat tgcaggatca ggaaaaaaga caggagtatt
    50161 ataaatagaa gaaacacgaa ataaaagagt cagtattttc taaatgaaac tttatgaaaa
    50221 caaaatgttt tttacaagaa aaagccttgc tttgtgagtt tatatatatt ataatgacaa
    50281 aggtgcgttt actttagtga attgcacaga cgtaatcaga atgatttcgt cggctaagaa
    50341 acgagaggtt gcgacacgcc cggacgcttt gccacgaacg agcgtgacgg aaattttcgt
    50401 ggagctatgt ctacttttca aaataggcga aggagggaac aagatggcaa aacaaaaaat
    50461 tcgtatccgt ttaaaagcgt atgaacaccg tattttagat caatcagcgg ataaaatcgt
    50521 ggaaactgca aaaagaactg gagctagcgt gtctggtcca attccattac caactgaacg
    50581 cagtctttac acaatcatcc gtgcgactca taaatacaaa gattcacgcg aacaattcga
    50641 aatgcgtaca cacaaacgtc ttatcgacat tgtgaaccca acaccaaaaa ctgttgatgc
    50701 tttgatgaag ctagacttac catctggtgt aaacatcgaa atcaaattat aaaactaaaa
    50761 caaaaataat ggaggtgtac tcatgaccaa aggaatctta gggaaaaaag tgggaatgac
    50821 acaaatcttc actgaatctg gtgaattaat tccagtaact gttgtagaag ctactccaaa
    50881 cgtagtacta caagtgaaaa caatggaaac ggatggctac gaagctattc aagttggtta
    50941 ccaagatatg cgtgaagttt tatcaaacaa acctgcgaaa ggtcatgttg caaaagcaaa
    51001 cacggctcct aagcgcttca ttcgtgaatt caaaaatgtt gagctaggag aatatgaagt
    51061 aggaaaagaa attacagtag atgttttcca agcaggagac attattgatg taacaggtac
    51121 tacgaaaggt aaaggattcc aaggggttat caaacgccac ggacaaagcc gcggaccaat
    51181 ggctcacggt tctcgttacc atcgtcgtcc tgggtcaatg ggtccagttg cacctaaccg
    51241 tgtatttaaa aacaaacgtc ttgctggccg tatgggtggc aaccgcgtaa ctatccaaaa
    51301 cttggaagtt gtacgtgtcg atgcagaaag aaatgttatc ttgatcaaag gtaatgttcc
    51361 tggagctaaa aaatctttaa ttactatcaa atcagctgtg aaagctaaat aattgtagga
    51421 agaaaggagg aactaaggaa tgccgaatgt agcattattc aaacaagatg gaagccaaaa
    51481 cggtgaaatc acacttaacg aagaaatctt cggaattgaa cctaatgaaa gtgttgttta
    51541 cgatgcaatc gtaatgcaac gtgcgtcatt aagacaagga acacacgcgg ttaaaaaccg
    51601 tagcgctgtt cgcggcggcg gacgtaaacc atggcgtcaa aaaggaacag gtcgtgcacg
    51661 tcaaggatct atccgctcac cacaatggcg tggaggtggc gtagtctttg gaccaacacc
    51721 acgttcttac agctacaaac ttcctaaaaa agttcgtcgt ttagcaatga aatctgtatt
    51781 gtcagaaaaa gttgctgaaa acaacttggt agcaatcgaa ggcttgaact tcgacgcacc
    51841 aaaaacaaaa gaattcaaac aagttcttgc taacttatct atcgactcta aagtattggt
    51901 tgttctagaa aaaggtaacg actttgctgc tttatcagca cgtaacttac caaacgtttc
    51961 tgtagtaact tctgataatg tgagtgtgct agacgttgta tcaaatacaa aagtgttagc
    52021 aacacaaact gctcttactc aaattgagga ggtgcttgca taatgaactt actagacgta
    52081 atcaaacgcc cagtgatcac tgaaaaatca atgcttgcca tggatgacaa gaaatacact
    52141 tttgaagtgg acactcgcgc aaacaaaact ttagtaaaac aagcggtaga agctgctttt
    52201 gacgttaaag tcaaaaacgt gaacatcgtg aacgtacgtg cgaaattcaa acgtatgggc
    52261 aaatatgcag gatatacaaa aaaacgtcgc aaagcgatcg ttacattgac tgaagattca
    52321 aaagaaatcc aattattcga agctgctgaa taagcactaa tagataactc attaaattag
    52381 gagggaaaaa cgtggcgatt aaaaagtaca aacctacctc aaatggtcgt cgtaacatga
    52441 caggttctga ttttgctgaa atcacaacat caacacctga aaaatcattg ttacaaccat
    52501 tgaaaaacca tgctggacgt aacaacaacg gccgcatcac tgtacgtcat caaggtggcg
    52561 gacacaaacg ccaatatcgc gtgatcgact tcaaacgtaa caaagataac gttgttgcaa
    52621 ctgttcaaac gatcgaatat gatccaaacc gttcagctaa catcgcgtta gtacactatg
    52681 aagatggagt gaaagcttac atcttagcac ctaaaggact agaagtgggt atgaaacttg
    52741 tttcaggtcc agaagcagat atcaaagtcg gaaatgctct tccattagaa aacattccag
    52801 ttggtactgt tatccacaac attgaaatga aacctggtaa aggtggacaa ttgatccgtt
    52861 ctgctggtac aagtgctcaa gtacttggta aagaaggcaa atacgtattg atccgcttga
    52921 actctggtga agttcgtatg atcttagctg catgccgtgc tacaatcggt tctgtaggta
    52981 acgaacaaca cgaattaatc aacatcggta aagctggacg ctctcgttgg atgcgtaaac
    53041 gcccaactgt acgtggtagc gtaatgaacc ctaatgatca cccacacggt ggtggtgaag
    53101 gtaaagctcc tatcggacgt aaagcaccag tttctccttg gggtcaacca gctcttggtt
    53161 acaaaactcg taacaaaaaa gctaaatcag acaaacttat tgttcgtcgt cgcaaaaata
    53221 aataattact ttgaccatgt gtcgcacatt ttgagaggag gttcaccatg ggtcgtagtt
    53281 taaagaaagg accttttgtc gatgagcatt taatgaaaaa agttgaagct caagcagggg
    53341 ttgaaaagaa aaaagttatc aaaacttggt cacgccgttc aacagttttc ccaagttttg
    53401 ttggatatac catcgcagtt tatgatggac gtaaacatgt accagtttac atccaagaag
    53461 acatggtagg acacaaatta ggtgaatttg cacctacaag aacttatcgt ggacatgctg
    53521 ctgacgataa gaaaacgaaa cgctaatttg aggggaggat aaatccaaat ggcagaacaa
    53581 atcacatcag ctaaagcaac tgcaaaaaca gttcgcactt cacctcgaaa agcccgttta
    53641 gtaattgacc ttatcagagg taaaagtgtc gcagatgcaa tttcaatctt gaaattcaca
    53701 ccaaacaaat ctgctggaat cattgaaaaa gtattgatgt cagcagttgc gaatgcagaa
    53761 aataactttg acttagatgt tgaaaacttg gtagtaagcg aagcttttgt taacgaagga
    53821 cctacaatga aacgtttccg tcctcgtgca aaaggatcag cttctccaat caacaaacgt
    53881 acaagtcata tcacggtagt cgtatcagaa aaataaggag ggataatcag tgggtcaaaa
    53941 agtacatcca attggaatgc gtgtaggcat catccgcgac tgggatgcta aatggtatgc
    54001 tgaaaaagag tatgctgagt tcttacacga agatttaaga atccgtaaat ttatttcaac
    54061 taaacttgct gatgctgctg tatcaacagt tgaaatcgaa cgcgcaacag gtcgcgtaaa
    54121 catttcaatt cacacagcta aaccaggtat ggttattggt aaaggcggtt ctgaagtcga
    54181 aagcctaaga aaagaattaa acaaattaac tggtaaaaga atccatatca atatcgtgga
    54241 aatcaaaaaa ccagatctag atgcaaaatt agtaggtgaa ggaatcgcac gtcaattaga
    54301 aaaccgtgtt gctttccgtc gcgctcaaaa acaagccatc caacgctcaa tgcgtgctgg
    54361 cgctcaaggg atcaaaactc aagtatctgg tcgtctaaac ggagcagata tcgctcgttc
    54421 agaaggatac tcagaaggaa ctgttcctct tcatacttta cgtgcggaca ttgattacgc
    54481 atgggaagaa gcagatacaa catacggaaa actaggagtt aaagtgtgga tctatcgtgg
    54541 agaaattctt ccaactaaaa aaaacactga gaaaggaggg aaataatcat gttagtacct
    54601 aaacgtgtaa aacaccgtcg tgaattccgc ggaaaaatgc gcggggaagc taaaggcgga
    54661 aaagaagtag cattcggtga atacggtttg caagctgttg attcacattg gatcacaaac
    54721 cgccaaatcg aagctgctcg tatcgcaatg actcgttaca tgaaacgtgg tgggaaagta
    54781 tggattaaaa ttttccctca caaatcttat actgccaaag caattggggt acgtatgggt
    54841 tctggtaaag gggcacctga aggatgggtt gcaccagtaa aacgtggtaa aatcatgttt
    54901 gaaatcgcag gcgttcctga agaagtagct cgtgaagcgt tacgtctagc ttctcacaaa
    54961 ttaccaatga aaactaagat cgtaaaacgt gaggaaatgg gtggtgaatc gaatgaaggt
    55021 taaagaaatc agagaattaa ccactgccga aatgcttgat caagaaaaac aattaaaaga
    55081 agaattgttt aatcttagat tccaattagc aacaggtcaa ttagaaaaca ctgcacgtat
    55141 taaagaagta cgtaaatcga ttgcacgcat caaaactgtg ttgcgtgaac aagctaagta
    55201 attatggaag gaggccatta agcgtatgac tgaaggaaga aatcaacgca aagtttatca
    55261 aggtcgcgtg gtttcagaca aaatggataa aacaattacc gttgctgtcg aaacaaagaa
    55321 aaaccaccct atctatggca aacgcatgaa ctattctaag aaatacaaag tgcatgatga
    55381 aaacaacaca gcaaaagttg gcgacatcgt gagaattatg gaaactcgtc cattatcagc
    55441 tacaaaacgt ttccgtttgt tggaagtagt cgaagaagca gttattatct aataacacga
    55501 gattttccta tatagattga atgcaaaatc tgaaaggagg atacacaacg tgatccaagc
    55561 agaaagtcgt ttaaaagtcg ctgataactc aggcgcacgt gaaattttga cgatcaaagt
    55621 ccttggtgga tctggtcgta aaactgctaa catcggtgat gtgatcgttg ctacggttaa
    55681 acaagcaacg ccaggtgggg ttgtcaaaaa aggtgacgtc gttaaagccg ttatcgttcg
    55741 aacaaaatca ggagctcgtc gtacagacgg ttcatatatt aaatttgatg aaaatgctgc
    55801 agtgattatt cgtgatgata aaagccctcg tggaacacgt atctttgggc cagttgcacg
    55861 tgaactacgt gaaaacaact tcatgaagat cgtttcacta gcaccagaag ttttataatc
    55921 ttgagacact tatcaaagga ggtgcgaaac agttatgttt gttaaaaaag gcgataaagt
    55981 taaagttatc actggaaaag acaaaaacaa agaaggcgtt gtactagcag cgttcccgaa
    56041 aaaagataaa gttattgtcg aaggtgtaaa tgtcgttaaa aaacaccaaa aaccttctca
    56101 agctgctcct caaggtggaa ttgttgagat ggaagcgcca attcatgttt ctaacgtgat
    56161 ggtggttgac tctactggtg tagctggccg agttggctac aaagaagtag acggcaaaaa
    56221 agtccgtgtt tctaaaaaaa ccggtgaagt tttagataaa taaaagttta aggaaggagg
    56281 gtcttactga atgaaccgcc taaaagaaaa atatcttaaa gaagtaactc catcattgat
    56341 ggaaaaattt aactatagct ctgttatgca aacacctaaa gttgaaaaaa tcgtcatcaa
    56401 catgggtgtt ggtgatgcgg tatcaaacgc taaaaactta gataaagcag ttgaagaatt
    56461 aactttgatc actggtcaaa aaccaatgat tacaaaagct aaaaaatcaa tcgctggatt
    56521 ccgtttacgt gaaggaatgc caattggtgc aaaagttaca ttgcgcggag aaagaatgta
    56581 cgaattctta gacaaattgg tttctgtttc actaccacgt gtacgtgact tccatggtgt
    56641 aagtaaaaaa gcctttgacg gtcgtggaaa ctatacttta ggtattaaag aacaattgat
    56701 cttcccagaa gtagattatg atttagtaga taaagtacgt ggtatggaca tcgttattgt
    56761 aacaacagca aactctgatg aagaatctcg tgagttgttg acacaattag gcatgccatt
    56821 ccaaaaataa ttaaaggagg cgaactacgt ggctaaaaaa tcaatgattg ctaaaaacaa
    56881 acgtcctgca aaattttcaa cacaagctta tactcgttgc gaacgttgcg gacgtccaca
    56941 ttcagtttat cgtaagttcc atctttgccg tatttgcttc cgcgaacttg cctataaagg
    57001 tcaaattccc ggcgtgaaga aagctagctg gtaaaaagta aattcgcata agaaggaggt
    57061 aaaagatcaa tggtcatgac agatccaatt gcagattttc taactcgtat ccgtaacgca
    57121 aacatggtta aacatgagtc tttagaagta cctgcatcaa aaattaaacg tgacatcgct
    57181 gaaatcttga aacgtgaagg tttcgttcgt gatgttgaat acatcgaaga tgacaaacaa
    57241 ggcgtgatcc gtgttttcct aaaatttggt aaaaacggcg aacgtgttat cacaaactta
    57301 aaacgtatct ctaaaccagg tttgcgtgct tatgtcaaag ctgacgaagt accaaaagta
    57361 ttgaacggtt taggtatcgc aatcatttcg acttctgaag gtgttgtcac tgataaagaa
    57421 gctcgtgaaa aaaatatcgg cggcgaagtt atcgcttacg tatggtaatc aagtagaatc
    57481 tacaaggagg tgtctttaag tgagccgtat cggaaataaa gtcgttgtta tccctgaagg
    57541 agtaacagtt actcaagacg gaaacaacgt tacagttaaa ggaccaaaag gtgaattgac
    57601 tcgtactttt tcagcagata tcaaaatgaa tatcgaagga aacgaagtaa ctttcactcg
    57661 tccaaacgac agtaaagaaa tgaaaacaat tcatggaaca actcgtgcga acttcaacaa
    57721 catggttgta ggtgtcagcg aaggtttcca aaaagcgtta gaactaatcg gggttgggta
    57781 ccgtgcacaa ttacaaggat caaaacttgt attgaacgtt ggttactcac atccagtaga
    57841 aatcacacca ccagctggag taactgttga agtaccagct aacacacaag taattgttaa
    57901 aggtgcgaac aaagaagaag ttggcgaatt agctgcaaac attcgtggaa ctcgtcctcc
    57961 agaaccttat aaaggtaaag gtattcgtta tgttggcgaa tatgtacgcc gtaaagaagg
    58021 taaaactggt aaataatcgt ttagaaaaat tgcatagaag ccagcagcag gaaaaaggca
    58081 tttggctgaa gtttaccttc aaaaaaatac atttttactt gtgccgcagc ttctattttt
    58141 cgaaacgtat ttactgcttg aaatagtagt acaataaaaa taaagaggtg acaattgtga
    58201 ttacaaaacc agataagaac aaaacacgtc aaaagagaca tcgtcgtgta cgtaacaata
    58261 tctctggtac tgctgagcgc ccacgcttga acgtttttcg ttctaacaaa aacatctacg
    58321 ctcaaattat tgatgacgta gcgggtgtaa cgctagcaag tgcctctacc ttggataaag
    58381 aaatttcagg tggaacaaaa acagaagcag cacaagctgt cggtaaacta gttgctgaac
    58441 gtgcagctga aaaaggcatt aaagtagtag tctttgaccg tggtggatac ctttaccatg
    58501 gccgtgtgca agctttagct gaagctgctc gcgaaaatgg actagaattt tagaagaagg
    58561 aggaatacca ttcatggttt atatcgatcc aaaacatttg gaattagaag accgcgttgt
    58621 tgcaattaac cgtgtaacaa aagttgttaa aggtggacgt cgtctacgtt ttgctgcttt
    58681 agttgttgtc ggtgacaaaa acggacacgt agggtttggt acaggtaaag ctcaagaagt
    58741 tcctgaagca atccgtaaag caattgaaga tgcgaagaaa aacttagttg aagtacctat
    58801 ggttggttca acaatcccac acgaagtaat cggtgctttt agtggtggta gaatcctaat
    58861 gaaacctgct gtagaaggtt ctggggttgc tgctggtgga ccagttcgtg ccgtattaga
    58921 attagctggg gtagcagata tcacttctaa atctttaggc tcaaatacac caatcaacgt
    58981 tgttcgcgca acagttgaag gtttgaaaca attgaaacgt gcagaagaag ttgcagaact
    59041 tcgcggcaaa tcagttgaag aattaatcgg ataaggagga caacaataat ggctgaatta
    59101 aaagttactt taaaacgcag tattatcgga cgtcctcaaa accaacgcga tacagttaaa
    59161 gcgttaggtc ttacaaagat taacagttca gtggttaagc cttctaatga agcaattaaa
    59221 ggcatgatca atactgtttc tcacctagtg gacgttgaag aaatttaatt tagaaaaatc
    59281 ataactagaa cattaaggag gtgccaaacc tatgaaactt catgaattaa aacctgctga
    59341 aggttcacgc caagtacgta accgtgttgg tcgtggtact tcatctggta acggaaaaac
    59401 tgccggacgc ggacaaaaag gacaaaaagc tcgttcaggc ggcggtgtgc gtttaggatt
    59461 tgaaggggga caaacaccat tgttccgtcg tttaccaaaa cgaggattca caaacgtgaa
    59521 ccgtaaagac tatgcagtca ttaatcttga tgtattgaac cgctttgaag acggagctga
    59581 agtaactcct gttgctttag ttgaagctgg aatcgtgaaa aacgaaaaag ctggaatcaa
    59641 agtattagct aatggggaac taaacaagaa attaactgtg aaagcagcaa aattctctaa
    59701 agcagctcaa gaagcaattg aagcggctgg tggatcaatc gaggtgatct aatgtttaga
    59761 ctattaaaag atgcttttaa agtaaaagac attagatcga aaatcctatt tactgtattt
    59821 gtgttgtttg tatttcgcct aggtgcacat attactgtac caggcgtaaa tgctgcttca
    59881 ttgcaaaacc tttcttcttt gccatttttc aacatgttga acttggtaag tggtagtgcg
    59941 atgcaaaact tttccatttt ctcaatgggg gtttcaccat acattactgc ttctatcgtc
    60001 attcagcttc ttcaaatgga catcgtgcct cgatttgttg aatggtcgaa acaaggggaa
    60061 gttggtcgta aaaaactgaa tcaagcgact cgatatctga cattagtatt ggcatttatc
    60121 caatctgtcg gtatcactgc tggattcaat caatacgctc aattaggttt cgtgaataat
    60181 cctaacgtag caacttacgt aacgatcggt ttgattctaa cagccggtac gatgtttgtt
    60241 acttggctag gagaacagat cacagaaaaa ggtatcggaa atggcgtatc aatgatcatt
    60301 tttgcaggta tcatttctag attgcctgaa agtatcagag aaatttatga agattacttt
    60361 ataaatattg atccttcaga catatggaaa tctgctattt ttgtggccat tttactagta
    60421 gcggtcttgt tgatcgtgac attcgtaaca tttttccaac aagctgaacg caagatcccg
    60481 atccaatata cgaaacgcgt agctggcgcg cctacaagca gctatctgcc gttgaaagta
    60541 aatgcagctg gagttattcc agttatcttt gctagttcgt ttattgctac gccaaatgct
    60601 gttttacaag cattgagcgg tagattcggt tctgaaaact ggtacaaagt gatgatggag
    60661 atctttaact ataatacagc acctggtgct acgatttata cgattctgat cgttgccttc
    60721 actttcttct acgcctttgt tcaagtaaac cctgagaaat tagcggaaaa cttgcaaaag
    60781 caaggaagtt atataccaag cgtacgccct ggtaagggga ctgaagagta tgtctcaaga
    60841 ttactgatgc gtttaagtac agttggtgca gtcttccttg gtttagtggc attgcttcca
    60901 atcattgctc aaatggtttg ggatcttcca caatcaatcg gattaggcgg tacaagctta
    60961 ctgatcgtta tcggtgtagc tttagaaact gcgaaacagt tagaaggctt aatgctgaaa
    61021 cgtaaatatg ttggatttat tgagtaagaa agcgtgttga ggtttcccct caacacacgt
    61081 tctcacacat ttattaggag ggaaaacaat gaacctcatt ttaatggggc tgccaggcgc
    61141 aggtaaagga acacaagctg aaaaaatcat tgatgcttat ggtattccgc acatctcaac
    61201 aggagatatg ttccgcgcag cgatgcaaaa cgaaacagct cttggcctgg aagcaaagtc
    61261 ttatatggat aaaggtgcat tagttcctga tgaagtaaca aatggaatcg tgaaagagcg
    61321 actagctgaa ccagatacag agaaaggctt cttattagat ggctttccac gtacgctaga
    61381 tcaagcaaaa gctctggacg caatgctaaa agatttgaat aaaaaaattg atgctgtcat
    61441 cgatatccat gttggtgaag aaatcttggt cgaacgtcta gccggccgat ttatctgcag
    61501 caattgtgga gcaacttacc ataaaatttt caaccctaca aaagttgaag acacatgtga
    61561 tcgttgcggt gggcatgaat tctatcaaag agaagatgat aaacctgaga cggttaaaaa
    61621 tcgtctggct atcaacatca aaaatagtga accaatcttg gcttattata aagaacaagg
    61681 tttgctaaac actattgatg gtgctcgtga aatcgatgct gtatttgcag atgtacaaaa
    61741 aatcattgaa aaatagcacg attgcccgta gccgacttgc attatgcagt tactcttgtc
    61801 aagttcaata aattatgata gaatatgtca gtccgacttc tagagaatcc tctagatgat
    61861 ccattttgag aattttgagg tgggaactgc agtttccgcc attttatcgt gtgaaaatgc
    61921 gaaacaagta caaggaggta ctgtgcgtgg caaaagaaga tatgattgaa gtcgaaggta
    61981 cagtcgtcga aactttgccg aatgcaatgt ttaaagttga actagaaaac ggacaccaag
    62041 ttcttgctac tgtttcaggt aaaattcgta tgcattacat ccgtatctta cctggtgaca
    62101 aagtaacggt tgaattgtct ccatacgatc ttaaccgtgg ccgtattact tatcgcttta
    62161 aataattgta ctccgtaaaa ctacaggagg tattatcatg aaagtaagac catcagtaaa
    62221 accaatgtgc gaacattgta aagtaattcg tcgtaaagga cgcgttatgg tgatttgccc
    62281 agcaaatcca aaacataaac aacgtcaagg ataattggag gtgctataaa tatggctcgt
    62341 attgcaggag tagatattcc tcgtgataaa cgtgtagtag tttcacttac ttatatctat
    62401 ggtattggta acactactgc taaaaagatc ttagctgatg ctggtgtatc tgaagatgtt
    62461 cgcgttcgtg aattaacgaa tgaacaaaca gatgctatcc gtgcagaagt agataaatta
    62521 aaagttgaag gggatcttcg tcgtgaagtg aacttgaaca tcaaacgttt gatggaaatc
    62581 ggttcttacc gtggaatccg tcatcgccgt ggattgcctg ttcgtggaca aaacacgaaa
    62641 aacaatgcac gtactcgtaa aggcccagct cgcgctatcg ctggcaagaa aaaataattc
    62701 ttaagtgaag gaggttaaaa cttcatggca gcaaaaaaag tgaatcgtaa acgccgtgtg
    62761 aagaagaata tagaagcagg tattgcacat atccattcta cattcaataa cactatcatc
    62821 atgatcactg atactcacgg aaatgcatta gcgtggtcat cagctggttc attaggtttc
    62881 aaaggaagca aaaaatcaac tccttttgcc gctcaaatgg cagcagaaac tgctacaaaa
    62941 gcagcaatgg aacacggatt aaagactgtt gaagtaacag ttaaaggacc tggttctgga
    63001 cgtgaagcag caattcgttc attacaagct acaggtttag aagtgactgc aattcgtgac
    63061 gtgactccag ttcctcataa tggtgcccgc cctccaaaac gccgtcgtgt ttaatgagtg
    63121 ccgctcattt tgagttttat cagtaacgtc attgaacaag acacttttcg ttttgaaagg
    63181 ggtaaagata agaatgattg agttcgaaaa accaagaatc gcaaaaattg atgaagaaaa
    63241 agattatggc aagttcatcg ttgaacctct tgaaagaggg tatgggacta ctttagggaa
    63301 ttccctacgt cgtattttat tatcttcttt gccaggtgct gccatcacta atattcaaat
    63361 cgatggtgta ctgcatgaat tctctaccat caaaggtgtt agagaagacg tcactcaaat
    63421 catcttgaac attaaaggct tagcgctgaa aatgtatggc caagaagaaa aaacccttga
    63481 aatcgatatt accggacctg ctacagtaac tgctggcgat attatcgttg atagtgatgt
    63541 agaaattctt aacaaagata tgtacatctg tagtgtctct gaaggagcta cgttccatgc
    63601 tcgcttaaca gtgaaacctg gacgtggtta tgttcaagca gatgaaaata aaaaagaaga
    63661 tatgccaatt ggcgttcttc cagtcgattc aatctacaca cctgttcgtc gtgtgaacta
    63721 tcaagttgag aacacacgtg ttggacaccg tgaagatttt gataaattga cgatggagat
    63781 ttggacagat ggttcaattg aaccgttgga agcaatgagt ttgtctgcga aaatcatgac
    63841 tgaacatttg gatatttttg tgaacttaac tgatgaagca aaaaatgctg aaatcatggt
    63901 cgaaaaagaa gaaacacaaa aagaaaaaat gttggaaatg acgatcgaag agttagactt
    63961 gtctgttcgc tcttacaatt gtttgaaacg tgcaggaatc aatactgtac aagaacttac
    64021 aaataaatct gagcctgaaa tgatcaaagt ccgtaactta ggacgtaaat cattagaaga
    64081 agttaaagca aaattgcacg acttaggttt aggtttacgt aaagaagatt aaaacctttt
    64141 acgaaggagg gaaaccaacg tgagttatcg taaactagga cgcacatcta gccaacgtaa
    64201 agcgatgttg cgtgatttaa caactgattt attaatcaac gaacgtattg tgactactga
    64261 agctcgtgcg aaagaagttc gctcaactgc agaaaaaatg atcactttag gtaaacgcgg
    64321 agatttgcat gcacgtcgtc aagctgcggc tttcgtacgt aatgaagttg ccagcgtgcg
    64381 tgaagaaaat gaggacatcg tcattgaatc agctttacaa aaactgttca acgatattgc
    64441 acctcgttac gcagaacgtc aaggcggata cacacgcatt ttgaagacag aaccaagacg
    64501 cggtgatggt gcaccaatgg ttgtcttaga acttgtctaa aatcacacta ataacatcac
    64561 tttatagatg attcttttag agtgttatga tgttggaaga tatttttccg agtctagctc
    64621 agcgctgctc ctcaagcgaa tatcgcttgg ggagtaagca ctcatcataa agtgtttttt
    64681 tataccttca attacctgtc atgacagata tgtttgaagg cataaaaaaa cattttaagc
    64741 gagaaataat tgaaaatttt gtcttttttt gatagtgtta gtaggaactt taggatgaag
    64801 aaggagtaat catggagcca attattgaat tagaaaagat aaattataaa taccagccag
    64861 atgatcttcg ccccgcattg aaagatgtct cctttacaat tgataaaggt gagtggatcg
    64921 ctattattgg acacaatggt tcaggaaaat cgacacttgc caaaacgatc aatggattgc
    64981 ttctgccaga atcagggatc gtgaaagtgg gaaatcaaat attggatgaa gaaaatattt
    65041 ggacgatccg acagatggtt ggaatggttt ttcaaaatcc agacaatcag tttgttggtt
    65101 cgacagtaga agatgacgtt gcttttggtc ttgaaaatca agggattccc agagaagaaa
    65161 tgctagtgcg tgtgaaagat gcacttgaga aagttagaat ggctgagttt gcttcacgcg
    65221 agcctgctcg tttatcagga gggcaaaaac aacgtgttgc cattgccggc gtggttgcct
    65281 tacgacctga tatcattatt ttagatgaag caaccagtat gctagatcca gaaggtcgtg
    65341 aagaagtgat ctctacgatc aagaagatca aagaagaaag tcaattgacc gttatatcta
    65401 tcactcatga tattgatgaa gcagctaatg ccaatcggat actagtgatg agacaaggcg
    65461 aacttgtccg tgaaggtaca ccaaaagaaa ttttttctgc tggtcctgaa ctgatcgact
    65521 taggtctaga tctaccattt cctgaaaaac tgaagagcgc actgaaagaa cgtggggttg
    65581 atgtgcccag tgaatatatg acagaagaaa ggatggtgga ttggttatgg acatccgttt
    65641 tgaacaagta gacttcactt atcagccgaa tacaccattt gaacaaagag ccttatttga
    65701 catcaatatg acgatcaaag aaaacagtta tactgcatta gtagggcata ccggaagcgg
    65761 gaaatcaacc ttacttcagc atttgaatgc gcttgttaag ccaacaagcg gtacagtcca
    65821 tattggagaa cgagatattc agccggatac ggataataag aatttaaagc ccatccgaaa
    65881 aaaagttggc atcgtctttc agtttccaga agcacagcta ttcgaagaaa cggtagcaaa
    65941 agacattgct ttcggtccta aaaactttgg tgtcagcgaa gaagaagcac tagtcctagc
    66001 aaaagaaaca ttagaacaag ttgggctgga tgaaagctat ttggaacgtt cgccatttga
    66061 actttcagga gggcaaatgc gtagagtagc aatcgctggt gtgcttgcca tgaggccgga
    66121 agtacttgta ttggatgaac caacggcagg acttgatccc cagggcagaa aagaaatgat
    66181 ggagatgttt tggcgtcttc ataaggaaca gcagatcact attgtattag ttacgcattt
    66241 aatggatgat gtagccaatt ttgcagatta tgtctatgtg ttggaaaagg gacgaatcgt
    66301 taatagcggg gagccgcaag aggtctttca aaacattgaa tggctaaaag aaaaacaact
    66361 aggcgtacct actgccaccg agtttgcaga agaattaatg gctaaaggga tgaactttgc
    66421 taccttgcct ttgacagctg aagaattagc agacgctatc gtccattatg ctggaggaaa
    66481 tgcccatgat gaataaattg atatttggtc gttatatgcc tggagattcc ttgatccatc
    66541 gattggatcc ccgagctaaa ttattagcca gtttttattt tatcggaatc atttttttag
    66601 caaataattg ggaaacgtac cttttgttgg catttttcac attactttct gtcttcttgt
    66661 caaaagtaga tttgaaattt tttatccgag gcattcgccc attgatctgg ctaatcctct
    66721 tcactgtagc tttgcaggtc ttgtttacaa gtggcggaga aacttattgg agttggggtg
    66781 ttttccatat cactgaattt ggtgtgatga acggcctctt tattttctgt cgattcgtgt
    66841 tgatcatttt tatgtcgact ctactcacgc ttactacacc gcctttggaa ttatcggatg
    66901 cgattgagta cttgcttcgt ccattgaaag ccgtacggtt cccagtccac gaaatttcat
    66961 tgatgctgtc gattgctctt cgttttgtgc ctacattgat ggacgaaaca gaaaagatca
    67021 tgaacgctca gcgcgctcgc ggtgttgatt ttggtgaagg aaacctagtt cagaaaatga
    67081 aagcagtcat tcctttgctg attcctttat ttgtcagcag cttcaaccgt gcggaagatc
    67141 ttgcgactgc tatggaagca agaggctaca aaggtgggga aggaagaacg aaatatcgtg
    67201 tattgcattg gcataataga gacacgatgg ttatcattgc ttttgtatta ttaactgtgc
    67261 tactgatctt tttaagagga taaacgttca gtgggccgct atctattctt ttcttgctga
    67321 tctgtttgca agaaaggatg caagcagtgt atgacaagaa atattagggt ctgtggcaga
    67381 ctaaggtcac aggcctcttt cttttatctg aaaagaaaga acgaaaaaat tttatagaaa
    67441 aatcattatt ccagataacc ttttaggaac aaaggagaga aaaacatggt acgttacaaa
    67501 gcaatcattg cgtacgacgg aacaaatttt aatggattcc aaaaacagcc aaatgggcgt
    67561 actgttcaag aagaagtaga aaaaacatta caaaaaatgg caaatggaaa agaaatcacg
    67621 gtctttggtt cgggaaggac agatgccgga gtacatgcga tcgggcaagt aatccatttt
    67681 gattatccag aggagcgtcc gttagaacga atgcgtttcg cgctagatac ccagtcacca
    67741 gaggatattg ctgtaagaca agtagaaatt gtttcagaag acttccacgc aagatatttg
    67801 gtcaaagaaa agacgtatca atttcgagtg gatataggga aaccacgcag tccttttcga
    67861 cgccattatg caagttattt tccttaccca cttgatttgg aaaaaattca gcgggcgctt
    67921 cctgatttat tagggactca cgatttcacc tctttttgtg catcaggaag ctcgatagag
    67981 gataaaatac gtacgatcta cgaggctaag atggaggtaa atgaaacaaa agacgaattg
    68041 ttgtttacat ttaggggaaa tggcttcttg tataagatga tacggatctt agtaggaacg
    68101 cttttgaaaa ttggcaatgg ccgattgcct gaagatagta tcccagatat tattgcgaaa
    68161 aaagatcgta atgcagcagg acctacagca caccctgaag gactttattt gtatgaagtg
    68221 aaatatgaat aagaggttat gatagaagtg gtcagcttca agaaataaga aggtatttac
    68281 gaaaattact tctcaaaatt ttgcaaaatt tcggcttatt ttcgaagaag ctacttctgg
    68341 aacaccgttt aagaaagagg tcgtgagcct gtcatttaac tctgagtcac aaagagcaat
    68401 ttcaaaaaac agcttctcat ctttgatgaa tatcgtgact tgtggacgag gcgttgataa
    68461 gtgaatcatc tttttgttac ctattttttc tgtaaccaaa aaacgatgga acgttgtttt
    68521 gattgccctt ggtgcggaca gtctgaacag gttccatcgt ttctttattt tctacgtctt
    68581 cttctgtgtg tgtttcgtgg ttgttcttct attgctggat caatcgtgcc ataaaagggg
    68641 cgattgcggt caaggtacca gacataaaga ataaacaatg caatacatag gacaaaaagg
    68701 attgcaccta tcgtaaatcc ttcaggcaag taactgaaag tgacagtatg tttacctgct
    68761 ggaactttta tcatcaagaa tgcgtctttg aaattgctga ccttgactgg tttcccatca
    68821 attttagctt tccatccttt gtcataagga atagttgtca ccaacatgga atcttcagcg
    68881 gtatttacag tcccagaagc ttttctgcca gtggcttcca tttcgacacc tttttctttg
    68941 atctgattca ttgcggcttg ataggctaca acatctagac caacgacttt aggctccatg
    69001 aaacttattt ctttcgtccc ataaaaactt gcagtaaatg tcactgttgt ctcttgagga
    69061 taatagccaa gatcatagta ttgaccagaa atgttgattt gcgtttttcg cgatgtacca
    69121 ttgacagtga tcgtcgcact agaactttct aattgcgcaa aattggttgg gaacaaactt
    69181 aagtaagctt gtgtatttgc aggcacatgg acttcccaag tgagctcttt tggctggtta
    69241 tctgctgttt ctgagtaagt gacgccagtc gttgtctttg tgatcgtcgt attcaactga
    69301 ttcttcaaag taggtgtata gaatgtaaaa tactgttcat ttagaccaga caatgtattg
    69361 atcaagtttg tctgactggt caaattatct gtcactggct gctcaacatc attaacggac
    69421 aacggtgcag taaatgctag tggcaatgca ttgacattct gatacagtgt atagtcgccg
    69481 ctttgaccag cttggcggaa gccatattta ctgaacgtgc tttcgttttt agaaatgtta
    69541 tatttgattc ctgtaaaccc atccatcagc aaagtattat tgttataacg gatattcaaa
    69601 tttgtccctc ttgaacggaa acctaattta tccaggtaag aggaagaatt acgatttctt
    69661 atagaagaaa acaaactgat accactgtaa ccatagttga tcgaatcgtt tgcagatact
    69721 ggatccagat tttccaagcg gtaaaacgag tcggaatctt tttttgtttc atttacaaga
    69781 gctttcaagt caggataagg atcagtatat aaactgcggg aagcataatt ccaatcatca
    69841 agaatgccat ggatcatact gttcgtattg accagtgctt cagcagacat gatcaataat
    69901 aacaatatac tcagatgatt gaatgaaagt tttttatgct gataaaatcc cacagcaaaa
    69961 gcataaatcc ctaagaaaac cactgtcaat acaaaggaag tcatcgtcac ataggtgtag
    70021 cttccttcag gtttgatgcc ccatgccacc gcaaagagag cagctacgac caaaatgatt
    70081 cctaacaaca gcccacgatc gtcttgttct atcttttccc atccttttcc tgcaagcagg
    70141 agcactaaga aagagaacag ataggcataa cggaataaaa acatattcgg tgcatgcata
    70201 ccatgccaga acaaattcaa tggaacaaga taaaaacttg cgataagtat ggcgaaaaga
    70261 ctgccgtaca aaaatttatt tttcaacggt actttccgac tggcaaaata aaacagacag
    70321 aggactaatg gtagcagacc tgcgtaaacg aacggaatcg atccgtattt tgtcgtgtca
    70381 taaacaccaa tcatattttt catgattaga tccagataag aagtcgcttc tgttttcaac
    70441 gtagtgatct gactcagtgc ctcgccattt gaccgaagat ccagcactgc tggcaataca
    70501 atgatcattg aagcgccgcc tgccagtaaa gaagtgatac cataaggaac aatcgttttt
    70561 ttatattctg accagtttgt caatagacgg gcgataaaat ataagaaaga aaatacgcca
    70621 atcataaaac ccatataaaa actggataaa aacaagagta aataactgat aaataaaagt
    70681 ttcggccgtt taagatccat aacacgatga atccccaaaa tcactaatgg aagataaaca
    70741 aatgcatcca gccacatgat gatctccgaa tgtgcagtga taaatgagat caatgcgtaa
    70801 ctgacgctaa gtgcaacatg cccccagcga ggaatcttga acgtacgtgc aagaaaccaa
    70861 aaagccaatc cggcacagcc gaccttaagc aaagtcagaa catacagtgc atctggcatc
    70921 atctggtttg gaaaaaagaa caccagtggc gtgaagatcc cgcctagata gtaagaaatt
    70981 aaagataaat aattcaatcc tagagaagca ttccatgtat aaaaaatact ttgctttcca
    71041 agaagaacat ttcggaagct ggcatgaaaa ttagaaaact gtgaaaaggc atcacttgcc
    71101 aaaatacttc tgctgcttcc aggatagatt ccgatactta gataaacaag tgctaaaatc
    71161 aaaaacggaa tcaggaaact agctgctgcg tagcgtccgt tttctctaaa gaatgacttt
    71221 aaattttttt tcatatttat tttccaccaa atttctatga agttgtattc agtttatcat
    71281 atccttgttt ttctctgctt aaaaagagtc tagttttcac aaaaaaataa aatttagaat
    71341 aaataacttc ataatttgtt cgtataacaa aaataaacgc aaaaaaaggg taggaatcaa
    71401 aagtcccacc cttaaaaatt tttattaaat aaaaaaccgt tcactatctg aacggtccaa
    71461 gtatcattca attaaacgcg ttcaactttt cctgatttca aagcacgagt tgacacccaa
    71521 acttttttag gttttccgtc gaccataacg cgaacttttt gtaagttagg tttaacagta
    71581 cgttttgttg agttcatagc gtgtgaacgg ttgtttccac tttttgtctt gcgacctgta
    71641 aagtaacaaa cttttgccat ggtaatttcc tcctttgcct tgatttggag caatagaata
    71701 attcagctca taccagacta acatatcata tagaatggga atttgcaagt acattttcat
    71761 agatacaatt gacaattgag aagaataatt gtaaattaaa caaggttata cctgaaattt
    71821 tgcaaggttt ttgagtactt tgggtaactg ccttttattt caagttgcgc tatgataaaa
    71881 tatgatagaa gaaaaatggg agttttttac ccgttaagga ggctcattca tggctgtaaa
    71941 aattcaaacg tcagctggaa ccattgaaat taccaatgat gtaattgcaa cagttgttgg
    72001 tggcgccgcg acagatgtgt tcggtgttgt aggaatggcc agcaaaaatc aaattaaaga
    72061 taatattact gagattttgc gtaaagaaaa ttactcaaga ggagtagttg tccgccaaga
    72121 agataatggt attgctgtag atgtttatac gattatcagc tatggtacga aaatctctga
    72181 agtttcccgc aacgtccaag aaaaagtgaa atacaatcta gaggcattat taggcgtaac
    72241 tgccaattca gttaatgtct ttgtacaagg tgttcgtgta ctgccagact aggcaggaga
    72301 ctggctggta actcagcagt cgatctgaag gaggattatc taggtgagtg taacagaaat
    72361 aagtgcaagc cagttccagg aaatggtcca ggcaggtgcg aatcgtctgc aagtaaatgc
    72421 agaatacgtc aattcattaa atgtattccc tgtgccagat ggcgatacgg gaacaaatat
    72481 gaatctatct atgactagtg gagctaaggc agtagtagat tcaacctcag aaaaagtagg
    72541 agaattagca gcaatgcttt caaaaggatt gctgatgggt gcgcgcggaa attccggcgt
    72601 tattttgtct caattgtttc gtggattttc taagcaaatt ccagatgttg tctcattaaa
    72661 tgcttctgat cttgctgcag ccttcactca tggagttgaa actgcgtata aagcagtaat
    72721 gaaaccagta gaaggaacga ttttgacagt agctagagaa gcagcgaaag ctggagaaaa
    72781 gaaagcaaaa agtactgatg atgtcgttga agtaatgaca gctgttgtaa aaggagcaaa
    72841 gcgtgcttta gctaaaactc cagacttgct tccagtccta aaagaagtcg gtgtcgtaga
    72901 cagtggaggg caaggactgc tgtttgttta tgagggcttt ttaagtgcat tgaatggaga
    72961 attccaagcg gatgaaacat atgaaccttc tccagcagaa atggatgata tggtcaatgc
    73021 agaacatcat cgcagtgtcc aaggtcaaat ggcgacagaa gatatccatt ttggttactg
    73081 tacagaaatc atggtaaaaa tcggagaagg acctacagtc gatagtacat tcgattacga
    73141 agaattccgt aactatttag atggtatagg agattcttta cttgtggtca atgatgacga
    73201 gatcatcaaa gtccacgtgc atacagaaaa cccaggagaa gtcttgaatt atggacaaaa
    73261 attcggttca ttagtcaaaa tcaaagtcga caacatgcgc ttgcagcatg agacgatttt
    73321 ggaacacgat gaagaagtga acgagtttgc gaaaaaagaa ataccacgta tcccgtatgg
    73381 tatcgtagcg atcgctgctg gtaaaggcgt ccaagagctg tttgaaagtt taggtgctaa
    73441 ttatgttatc agtggtggac aaacaatgaa tccaagtacg gaagacattg ttaaagcgat
    73501 tcaagaagta aacgcagata aagtgattgt cttgccaaac aataagaaca tcttcatggc
    73561 agctgatcaa gcagcagaag tagcagatat cccagtagca gttgttcctt caagaacgat
    73621 ttcccaagga ttgacagcta tgctttcatt caatgatcaa gcttctttag aagaaaacaa
    73681 agaaacgatg acagaaatgt tgtctagcgt tgtgagcggt caagtcactc acgcgatcag
    73741 agatacctca attgacggtg tagagatcaa agaaggagac tttttgggca tgatcgatgg
    73801 aaaaatcctg atctctaacc cagatatcac agcaacagcg ttaagtacac tagaacaaat
    73861 gatcaatgag gacacagaga tcgtaacgat cttggcaggt gatgaaggaa cagttgaaca
    73921 agcaaatgaa tttgctgaga aactaacaga aaaacatcct gatttggaaa tcgagatcca
    73981 ccaaggagat cagcctgtct atccttatct attttctgct gaataatttc tgtgtcagaa
    74041 tatctttcta acacagtatt cattcataaa aggccttcgg ttagtgatca ggtatgatcg
    74101 ctgaccaagt cctttttttg ttaaaggagg gaggaaaaat ttatgcgttc tttagatgat
    74161 ccaatcacta tgctggcagg agtcggagaa aaaagagcag ctagtcttgc cagcttgggc
    74221 atccaaacga ttgaagactt actgacttat tatccttttc gctacgaaga tattcaggaa
    74281 agaaatctaa acgagatcca ggatcaagaa aaggttactt tgaaaggaat cgtagtctcg
    74341 cctcctgtga tgaatcgatt tggttataaa aaaagccgat tgcaatttcg tatgatgcaa
    74401 gaacatgatg tgttcaacgt ttcttttttt aatcaacctt atctaaaaga aaaagtcatt
    74461 ttatcggagg aaatcgccgt atacggtaaa tgggatgcaa agcgtaaagc cttgaacggg
    74521 atgaagattt taggttctca aacaatggat gacttttcgc cgatatatca cgtcaacaaa
    74581 tcaattcgtc aaacgacatt agttgatcta atcaaaaaag ggtttcaaga atacggcaca
    74641 ttgattgaag aaaatctgcc aaacaacctt gtggagaaat atcgactgct tgaccgagct
    74701 tctgctgtaa gaagtatgca ttttccaaaa gaccacgaag aaaaccatca agccaaacga
    74761 cgtgtcgttt ttgaagagtt ctttttattt caaatgcgtc ttcaaggatt gaaaaaagca
    74821 gaaaaagcag aaacgaatgg tttggagatc ctttatgatg tccaaaaatt gaaacaattt
    74881 actcaaaagc taccttttga actgacaaat gcacaaaaac gtgtaacgaa tgaaatctgt
    74941 cgggatcttc gcagtccgca gcatatgcag cgacttttgc agggagatgt aggtagcgga
    75001 aaaacagtgg ttgccgcgat tgcgttatac gctaccgtta cagcaggttt ccaaggtgcg
    75061 ttgatggtgc ctacagaaat cttggcacaa caacatatgg aaagcttgca acagctgttt
    75121 gatcccaatg aagtaaccat cgcattgatt acggggtcaa cgaaagcaaa agaacgacgt
    75181 gagctccttg aaaaactaga gcaaggggaa attaacgtcg tgatcggcac acatgcattg
    75241 atccaagatg gagtcgaatt tcagaattta ggtctagtta ttacggatga gcagcaccgt
    75301 tttggcgtga accagcggaa agtacttcgt gaaaaaggct ggcgaccaga tgtcttattt
    75361 atgacagcga ctccgattcc tagaacatta gctattacag catatggtga gatggatgta
    75421 tctatcattg atgaacgtcc agctggacgt atcccgatcg aaacacgctg ggtaagaaca
    75481 cctcaattgg acagtgtact ggattggacg aaaaaagaac tggcaagagg acatcaaatg
    75541 tatgtcatct gtccgctgat tgaagaatca gaagcattag atgtaaaaaa tgcagtggaa
    75601 atctatgaaa aacttcgtga actatttgca ccaaaatatg aagttggctt gttacatggt
    75661 aaaatgaaga atcaggaaaa agaagtaatc atggaagcct tcaaagacaa ccagatgcag
    75721 atattagttt cgactactgt aattgaagta ggggtaaacg tgccgaacgc gaccgtcatg
    75781 ctgatcatgg atgcggatcg atttggattg gcacagctgc accaacttcg tggacgtgtt
    75841 ggacgcggaa gtgatgcttc ctactgtatc ttagttgcta atcctaaaaa tgaactaggt
    75901 gtggaacgga tgaaaatcat gacagagacc accaatggtt ttgtactaag cgaaaaagat
    75961 ctggaattac gtggtcctgg tgaagtattt ggcttccgtc aatctggtct gcctcaattt
    76021 gcgattgctg acttggtgac ggatggaaat gttttggagg tagccagaga agaggctgca
    76081 gccctttggc aaataaagga ttggcagatg cttcctgagt atcagccttt agcgcaaagc
    76141 ctccaaatag aaaatcagga taatcgttat ttcgattagc agactgtgct atactttata
    76201 ctgatgacgg aaaaaggagt ggaaacaatg aaaattgccg tagatgcaat gggcggagat
    76261 cacgcacctc aagcaattgt agaaggcgtg gctttagcgc agcaagattt tccagaagta
    76321 gaattcttac tttacggaaa agaagcagaa atcaagaaat acttaacaaa tgaaaaaaac
    76381 atcacgatta tccatacaga cgaaaaaatc aatagtgatg atgagccagt aaaagcaatt
    76441 cgacgaaaaa agactgcatc aatggtcctt gctgcacaag cagtgaaaaa cggtgaagca
    76501 gatgctgtat tttcagcagg gaatacaggc gcgctactag cagcagggtt gttcatcgta
    76561 ggacgtatca aaaatatcga acgccctggc ttgatgtcta ccttgccagt agtagggaaa
    76621 gaaggtgccg gtttcgatat gctggatctt ggcgctaacg cagaaaacaa accagaacat
    76681 ttgttgcaat acggtatttt aggttctttt tatgcacgta aagtccgagg tatcggacgt
    76741 cctcgtgtcg cactcttaaa caacggaaca gaagcaacaa aaggtagtga agtgacgaaa
    76801 aaagcctatg aactccttca gaatgaaaca agcttgaatt ttattggaaa cgtagaagct
    76861 cgtgagctac tcaatggtgt ggctgatgta gtggtcacag atggatttac aggcaacgct
    76921 gttttgaaat ctattgaagg aacagcaatg aatatgatga atctattaaa atctgctatt
    76981 ctaaatgaag ggatcaaagg aaaaatgggc gctatgcttt taaaagacgg cttacgttct
    77041 ttgaaagcag aaatggatta ttccaaacat ggcggtgccg ttttgtttgg cttgaaagca
    77101 ccagtcatta aaactcatgg agcaacaggc cccgatgctg ttcgctatac gatccgccaa
    77161 atccacacaa tgcttgagac agatgtagtt ggacaattag tcgaacaatt tgaaaaaagc
    77221 gaagaatagt atttggatat ctgaagcgat ccgttccatt gaaaaaaagg tggacattcc
    77281 tgaaaaatgc cagtaaaatt agccatggtg taggtaattg acaagtgtat gattcttgga
    77341 ggtgaaaaag ttgactcgcg aagaagtatt taacaaagta gcgaaaatta tttcaaatca
    77401 ttttgagata gataccgata aagtgacgga tgaattgaac atcaaagatg atttaaaagc
    77461 tgattcaatt agtattatgg aattcgtttt agagcttgaa gatgaattcg gtacggaaat
    77521 ttcagatgaa gacgctgaac aaattgaaac agtcggtggc gcagtagatt atatcagcag
    77581 ccatcttaaa taatttcaaa taaaagcaaa gaaggataaa aagctgcatg cagcttttta
    77641 tccttctttt tttaatcttt ttgacacgta aaagtcattt ttctgataat tctggcgata
    77701 aacagaaatt atgttgcaaa gtaaagcggt tttctatata attattaata attcattaca
    77761 caaagataag aataaccttt gttataaaac gatttattct gtgtaatgtc cttttttatt
    77821 aagaaaaact tccatttagg aagcagagag gggtattcga ttgtccgata accaattatt
    77881 agaagttcag aacctacata caggattccg actcaaagat gactattacg atgcagtaga
    77941 tgacgtttca tttactttag acaaaaacga gattttagca atcgttgggg aatctgggtg
    78001 cgggaaaagt accttggcta ctacgattat gggattactt gatcctaata acacaaaaat
    78061 tacaggtgag attttatata acgatctgaa tttaatcgac ctgaacgaaa cgctctataa
    78121 taaaatccgc ggaaatgata tcggaatgat tttccaagat ccgttgtctg cattaaatcc
    78181 tctcatgaga atcgaagacc agatcaaaga aggactttcc tatcacacaa agatgaatgc
    78241 agaacagcgt caagctcgtg cactagaatt gctagaacaa gtagggattc ctaatcctgc
    78301 gagagttgga cgccaatatc cccatgaact atcaggcggt atgagacaac gtgtcatcat
    78361 cgcaatcgca atcgcctgca aacctccaat cgtcattgca gatgaaccga cgacagccct
    78421 agatgtaacc atccaagcac agattcttga tttactgaaa gaccttcagg aagaaacaaa
    78481 aacagggatc atcttgatca ctcatgactt aggagtcgta gcagaaatgg ctgatcgagt
    78541 ggcagtgatg tatggcggac agttcgttga agtagcaggt gtaaaagaac tgtttgatca
    78601 tcctaagcat ccttatacac gctctttact gaactctata ccacaagaag gaaatcatga
    78661 tgcagaactc catgtgatcg aaggtgtcgt tccttctctg aaaaacatgc ctcgtaaagg
    78721 ttgtcggttt gcaccacgta ttccatggat accagcaagc gatcatgaag aagagcctat
    78781 ccttcatgaa gtcgcaccga accacttggt acgttgctcc tgctataagc atttccactt
    78841 tagagacgaa aaaggagagg tgtagatgac agaattaatc acaatcaaag acctgaaagt
    78901 tcattaccct attcgcagtg gattcttcaa tcgagtaacg gatcatgtat atgctgtaga
    78961 tggtgtggat tttattatcg aaaaaggaaa gacatatggt ttagtgggag agtctggctc
    79021 tggaaaatct actacgggaa aagccgttgt gggattagaa aaggtaacag ccggacaaat
    79081 catgtatgaa ggaaaagatg taacgaagcg aagcaaccgc aaaaaaatgg gttataacaa
    79141 ggatgtgcag atgatcttcc aagattcaat gtccagtttg aatcctaaaa agcgagtgtt
    79201 ggatatcatt gctgaaccca tccgaaattt tgaacggtta agcgatcaag aagaaaagaa
    79261 aaaggtaaaa ggactgctag atatcgttgg gatgccggaa gatgcgctat ataaatatcc
    79321 tcatgaattt tcaggcggac aacgtcaacg tctaggagta gcccgtgctg ttgctaccaa
    79381 tccaaaactg atcgtagccg atgaacctgt atctgctctg gatctatctg ttcaagcgca
    79441 agtattgaat ttcatgaaac gaatccagca agaatacggc ctcagctatt tattcatttc
    79501 acatgattta ggtgtagtaa aacacatgtg cgacaatatc gcaattatgt acaaaggtcg
    79561 atttgtagaa atcggtacaa gagaagacat ttataatgac ccgcgacata tctatacgaa
    79621 acgtctgctt tcagctattc cgcaaatcga tgtagaaaat agggaaagcc acaaagaaaa
    79681 tcgacgaaga gtagaacaag agtatatcca aaaccaaaaa gaatactatg atgcgactgg
    79741 tcgagtctac gatttgcgta cgatcacaac tacacacaaa gtggcgttga aagatggagg
    79801 tgcaagctaa tgtggaaaac gattctgcga cgtgttttat tgatgatccc gcaagttatt
    79861 attttaagtg ttctgatctt cttattagcc caagcgatgc cgggggatcc atttaccggt
    79921 ttgatcaatc caaatcagga tccggcagtg atcgaacaga tgcgacaagc agctggcttg
    79981 aatgacccat ggtatgtcca gtacttccgt tggatcggaa atgctttgca aggcgatttt
    80041 ggtcaaagtt tcttatacaa acgctctgtt gctgacttga tcggcgaacg gatcatcaac
    80101 actttatatc tatcgatctt gacagttgtc cttacttatt tgattgctgt gcctctgggg
    80161 atgcttgctg gccggtatca aaattcaatc ttggataaga ttgtcgtaat ctacaacttc
    80221 tttagttttg ctgttccttt atttatcttc ggcttgatca tgctatttgt ttttggttat
    80281 cgtttaggct ggttcccaac aagtggatca caaacactag gtttgcaagg tggatttatc
    80341 actcaatgga tagaccgttt gaaatatatt ctgttaccat caattaccca agcatttttg
    80401 gcgactgccg tcacaatcca atatctgcga agtgaagtaa tcgattcgaa atcgctcgat
    80461 tttgtcagaa cagcccgttc caaaggggta ccgacgaata aagtattcag tcgacatatt
    80521 ttccgaaatg cagcgttgcc gatggcttct caaatggggt atgaaatcac ttcgttgatt
    80581 gctggttctg tagttatcga aaagattttt ggttatccgg gagtcggaaa actatttatt
    80641 gattccatcg gccaacggga ctataccgtg atcacatcct tagtattgat tttaggaatc
    80701 gcgacgctag tggggacatt actttcagat atcatcatga gtatcgttga cccgcgtatt
    80761 cggattcaat aaaaccaatc agaaaggaga atggatcgat gagtgaaaaa aacaatacag
    80821 cagttcaaga aagtattcct cctatgggga tacgaatgat tgctcgagaa tttaaaaaag
    80881 ataaaattgc tattttttca ttaggtttgc tagtggtttt attattagtg atctttatcg
    80941 gtgcgatgct gaccgatcaa gacaaagtga tgtatgttag tatcttcgac aaatacgctg
    81001 caccaggaac agtttcgaca caaggacaaa agttcattct gggagcagat gaaggcggac
    81061 gtgatgtatt aggacaattg atcataggtg ctagaaactc tgttacgatc ggatttgcga
    81121 tcacgatcct tacttcgatc atcggggttg gattagggat catctctggc tactacggag
    81181 ggatcattga caacatcttg atgcggatcg ttgattttat catgattcta ccgatcatgc
    81241 tgatcattat cgtcttcgta tcgattattt ccagatacaa tgtctggtcc tttatattca
    81301 tcatgagtgc attttattgg gtagctaaag cccgattatt ccgaagcaag acattatctg
    81361 aagcaagaag agattatgtc agtgcctcaa aaacaatggg aaccaatgat tttaaaatca
    81421 tgttccgcga aatcatgccg aatctaagtt cattaatcat tactaatttg acgatgaact
    81481 ttgcggcaaa catcggtatc gagacaactt tgactttctt aggtttcggc ttgccggcaa
    81541 atgtacctag tttaggtaca ttgatcggtt atgcaagtaa cggagaagtc ttagtgaatc
    81601 ggcaatggat ctggttaccc gcatcaatcc ttattttagt tttgatgttg agtatcaatt
    81661 atgttggaca agcattcaag cgttctgcag atgcgcgcca acggttaggc tagtatagaa
    81721 gcgaaggggg aaataggaat gaagaaaacg atttttggat ttgcagcgct gatgtctgct
    81781 gtcgttcttg cagcatgcgg gggcggaggt acagatacct ctggaaacaa ctctggaggc
    81841 aacggcaata acaaagagga attaactttt ccaattgcaa caaaaaataa tgatgatgca
    81901 atcgaagacg gcgaattaga agctgcggtc gcaacggaca gtcagtttaa aggattgttc
    81961 cagtgggagt tttaccaaga ttcatatgat gcagctttta tggctccttc tcatgaaccg
    82021 ctatttgcaa acgatgacga tttcagaatc acagacgacg gagctgcaac gcttgatcta
    82081 gatgaagata cgaaaaaagc gacaatcaaa gtaaaagaca acgtcaaatg gtcagatggt
    82141 gaagatgtaa cggcggacga tgtgattttc ccttacgaaa tcattgggaa taaagattac
    82201 actggtatcc gttatgacga cactttccgt aacatcgtag gtatggaaga atatcatgat
    82261 ggaagtgccg acacaatctc cggaatcaaa aaaatagacg acaaaacagt tgaaatcgaa
    82321 tataaagaag taaacccaag catgcttcaa tcgggtggtg gtatctggtc atatgcagca
    82381 ccaaaacata cgttagaagg tgtagcaatc aaagatatgg aatcaagcga tcaagtacgt
    82441 aaaaatccag tgacttttgg cccttattac atgagtaata tcgttgcagg agaatcagta
    82501 gaatacttgc caaacgagca ctactggaat ggtacgccta aattgaaaaa gatcacgatg
    82561 aaatctgtac cgatggcaag tgcaactgaa gcattgaact ctaaattata tgatatgatt
    82621 ttccaaatgc caacagatac ttacgatacc tataaagaaa ttgctggcta tacaaactta
    82681 ggccgtgaac aaacttctta tacttatcta ggattcaagt taggtaaatg gaatgcagat
    82741 acaaagagtg tggaatacaa tccagattca aaaatggctg ataaatcatt acgtcaagca
    82801 atgggctatg cattagataa taatgcagta ggtgaacgtt tctatgcagg tttgagaagc
    82861 aatgcgactt cattgattcc tccagtattc cagtcatacc atgatgatga gatgaaagga
    82921 ttcacacaag atctagacaa agcgaaaaaa ctattagatg atgctggcta caaagatgtg
    82981 gatgacgatg gtttaagaga agacaaagat ggaaataaac taacaatcaa ctttgcttcg
    83041 atgtccggtg gggaaacagc gcaaccattg gcagattact atatccaaca atggaaagaa
    83101 attggtttgg atgtccaata cacaacaggt cgtctgatcg aattcaattc tttctatgat
    83161 aaattagaaa acgatgatcc tgaaattgat gtttaccaag ctgcttggaa tacaggaact
    83221 gatccaaatc caagtggact atggggacca aatgctgcgt tcaactacac tcgttatgaa
    83281 tcagaagaaa atacaaaatt gatcaacgat atcagttcaa gcaaagcatt tgatgaagag
    83341 taccaaaaag aagcatatca aaaatggcaa caatatgcat ttgaagaagc atttgcgatt
    83401 ccaacgttgt tccgtaatga agtattgcca gtcaacgacc gtgtcaaaga ctgggattgg
    83461 tcttatgatg taccaaaccc atgggcaaat gtttctgtta cagcagattc acgttcataa
    83521 tcactaaaaa tgaccgaaaa agggcgatgt cctttttcgg ttttttgata tttttctgtt
    83581 tatttctcta atattcaggt caaggcgctg atttcatgac ttctccaata aacggtgttc
    83641 aaagtccagt ttctcggtat taagtcaaat ttatgcaaaa atatggaaag ccattttcac
    83701 aaaatttttt cttcatactc gaaactaaac gaatttttca caacttctcg tcaatcacta
    83761 ggattattaa agctttactt accttttgac cgaaccattt caaatttgac aaaaaaccag
    83821 tataataagg aagtatcgaa aaaatatcca taaaaggaat gtggaaaatg gacaaccagc
    83881 tgacaaaaga actaaaagaa aactataaca tcgtttttca cgatctaaat ttgcttgaac
    83941 aagcttttac ccattcatcc tatgtgaatg agcatcgcaa tctgcaatta tcggataatg
    84001 agcgacttga atttttagga gatgccgttt tagaactgat ggtttctcag tatttattcc
    84061 gtttatatcc tcatttgcca gaaggaaaat tgacaaaaac acgagctgcg atcgttcgcg
    84121 aagacagctt gtcgaaattt gccaaagaat gtcactttga tcaatatgtt ttgttgggca
    84181 agggagaaga gaattcagga ggacgtacgc gaccagcatt gctttgcgac ctattcgaag
    84241 cttttttagg cgcgttatat cttgaccagg gattcgacac gacccatgca ttcatcgaaa
    84301 aagtgatttt tcctaaagtc aaagcaggtg ctttttcaca tgagatggat cataaaacaa
    84361 aactacaaga agtcttgcaa aaatcaggtg atgtatcaat cgaataccga ttgatcaatg
    84421 aagaaggtcc agctcatgaa cgagtatttt ggatcgaagt ttatgtagat gatcaattga
    84481 ttggcactgg acaagggaaa tcgaaaaaat tagcagaaca agctgcggcg gaaaatgcat
    84541 tggcagcact gtaaaagtaa gtgagggaaa gctttgtatt tgaaacgaat tgaaatagcc
    84601 ggttttaaat catttgcaga caagacagtc attgattttg aaaatagcgt cacagcagtg
    84661 gttggaccaa atggcagcgg aaaaagcaat attacagagg ctatccgctg ggtattagga
    84721 gaacaatctg ctaaaagtct gcgaggcgga aaaatgccgg atatcatctt tgccggttcc
    84781 gatacccgta agcagctgaa tattgcagaa gtcacagtga ttttagacaa tagtgatcat
    84841 tatctgccat tggagtacaa tgagatcagt gtaacccgcc gttatcgacg gactggggaa
    84901 agcgagtttt tcatcaataa acaaagctgc agattaaaag atatccaaga attgttctta
    84961 gattctggat taggaaaaga atcgttctca atcatttctc aaggaaaagt agaagcgatt
    85021 ttcagtagta aaccggaaga ccgtcgtggg atctttgaag aagcagctgg tgtcttgaaa
    85081 tacaagcagc ggaaaaagaa agcagaacag aagctttttg aaacagaaga caacctcagc
    85141 cgcgtccaag acatcatcca cgaattggaa gaacagttaa ctcctttggc cgcccagagt
    85201 gaggcagcaa aggagttttt acggctaaaa gaaacactga ctcaaaccga tgtctctttg
    85261 atggttgctg aaatcaagac agcaaaaaaa gattgggaca acaaacaagc acagcttgcg
    85321 aaattcaatc tagaattagg caaactatct gagtcgatcc aagaacaaga atccattttg
    85381 gcgaagcagc gtaaagaaaa tgcgcaagcc gatcgtctga tcgaaaaaaa tcagcaagta
    85441 ttattagatc tttcggaaaa actgaagcag acagaaggac aaaaagatgt tttgcaggag
    85501 cggacaaagc atacacaaaa aagctcgcaa gaatatcaaa cttcattagc agaagcacaa
    85561 aaaaaggtta aacattttga aaagcttcaa gaaagcttga tgaaagcagc agcagaaaaa
    85621 gaaacagaga tccaaaaagc tgaagcaaat ctcataaaaa cacagcaaga attagaaaaa
    85681 taccaaaaat caacgaaaga attattggca gaattaagag atcaatatgt tgatttgatg
    85741 caagaacaag cagctgtagg aaacgaactg aagtatctag aacgtcaata tatacaagaa
    85801 acagcaaaaa gcaagcagac gttagccaag caatcggaag tagaagcatc agtagaccga
    85861 ttgatcttac aaaaagaaga attgacacaa aagcaggcgc agctaaaatc atctttaact
    85921 gaaacaaaag agaagttaga aatgatccaa caaaacggga aaaagttcca agaagcatta
    85981 gcaaaagaac agcctaaaat gtatcaatta atgaatcaag tccagcagct acgtgctcgt
    86041 aagaaaagcc ttcaagaaat ccaagaaaac tattttggtt tttaccaagg ggttcgattg
    86101 gtcttgcagc ataaacagca gctttcgggt attgtcggag cagtggctga actgatcgat
    86161 gttcctgcag atttcacgtt agctatcgaa gcagctttag gtggggcagc ccagcatgtc
    86221 atcgttgaaa atgaaaaaga tgctcggcaa gccatcacgt atttgaaaca gcaaagagga
    86281 ggaagagcaa cattccttcc actgacgaca atcaaaccaa gacaattacc ggctcatata
    86341 ttgacacaag cagcagctgt agaagggttt atagggattg ccagtgaaca agtcagttat
    86401 cccgatcaaa tccaaactgt ggttcaaaac ctgctaggaa cgatcctttt agccaaagac
    86461 ttaactagtg ccaatgcaat cgcgcaaacg atccgctatc aatatcgagt ggtttcctta
    86521 gaaggtgatg tgatgaatgc cggaggatcc atgacaggtg gagcgaataa acgaggaaac
    86581 caaggcagtt tgtttgtaca aaatcaagaa ttgaaacagt tgactagtga atttgaagaa
    86641 gcagataaac aactccaagc acaagaaaaa aaagttcagg agctgcaaca agaaacagct
    86701 cgtttagccg aagaacaaga agttttgcgt acacgaggcg aacaattacg ttttgaagaa
    86761 caagaagcga caaatcagct acaaaacatc ataaatgaac ttgaacgttt tgaaaaagaa
    86821 aagcaaatct caacttttga gacacgtgaa ctgcagcagt ttatcgaaga ttacgaaaag
    86881 caaacgaacg aattgaaaga caaacaaaca gatcttgaat cgcaacgaca aaaaatcgac
    86941 gaagaaatca aatcgttaag tcaagaaagc gaccagatgg aagccagaag agcacaagta
    87001 caaagtcaaa aagcacaaga acaagcagat cttgctgtct tgaaagagca gtttaatcat
    87061 ctgcagatcc agctccgcgg tgcccgagtc caaaaagcag aggcaaccga acgtcaagaa
    87121 gcgatagaaa agcagttggc gacattgaca gcagactttt ctgatcatga agtcacagaa
    87181 gaaagtttgg aaaaacaaat taacgaatta tctgctcagc gagaaacatt aaaagccgag
    87241 ttggcaaaag ccaaagagca gcgagataga aggcaaaagg agatcgataa gcttgaagca
    87301 gtcttggcag agcgtaatcg tgagcaaaaa gcacgactgt cggaacaatc caaattagaa
    87361 gtccaaaaag accgagctga aatgatttta gataatcatt tgacctatct gcagtcagaa
    87421 tatcagctga catttgagaa agcttcccaa gactatcaag aaacaaccga tatcgaagac
    87481 agtagaacaa aggtttcatc cttaaaagaa cagattgaaa aactgggacc agtaaactta
    87541 aattcaatcg aacaatacga acaagtcagt gaaagacata cattcttagc aacacagcga
    87601 gatgatctgt tagcagcgaa aaaccaattg tttgaaacga tggatgagat ggatgatgaa
    87661 gtgcggacaa gattcaaaga agtatttgaa gctatccgtc aagaattcaa aattgtcttt
    87721 ccaaatatgt ttggcggcgg taaagctgaa ttagtactga cggaaccaac tgatttgctg
    87781 aaaacaggta ttgagattga agtacaacct cctggcaaaa aactgcaaag tctaagtttg
    87841 ctttctggag gagaacgagc acttacagcc atcgcgttgc tcttttccat tatccgtgtc
    87901 tgtcctgttc cgttttgtat cttagatgag gtagaagcag cgttagatga agccaacgtg
    87961 aaacgttttg gtcgttactt gagcgatttc caagatgaca cacaatttat cgtagtcaca
    88021 caccgcaaag gaacgatggt cgcagcagat gtactgtacg gagtgacgat gcaggaatcc
    88081 ggtgtatcaa aaatcgtttc tgtacggatg gaagatatca atgaagaggc gaaaatcaac
    88141 gaaacgaaag tgtaggaacg aaaatgatca aattagtagc aatcgatttg gacggtacat
    88201 tattaaatga acaaaagctg atttcagatg agaataaaca agcattagca caagcaaaac
    88261 agcaaggggt caagatcgtt ctatgtacag gacgcccctt agctgcgatg gcgcattatc
    88321 tgcaagaatt aggactggta gatgaaggtg actacagcat cactttcaac ggcggtttgg
    88381 tacaaaaaaa tgatactggt gaaatcatgg aaaagaaagt gatggaagta tcagatatcc
    88441 aacgcttata caaactggct cagcaattgg atctgccact agatgttttg tctgacaatg
    88501 tggttttaca gctgcctagt gccccaagga agcaatcctt gtacaatgtg ttgaataagc
    88561 tgctgcaatt ccaaccagcg gatataagcg atattacaga tgaatttgtg cttaataagg
    88621 cagtaatcgc ttatgaccaa gaagaattag atcctaaaat caaagaaata ccagcagaat
    88681 tccatgaaat gtatgagatc atcaaaacaa gaagtatgct tttggaattt atgccaaaag
    88741 gtgttacaaa agcttatggt atttctttat tggcaaaaga tcttggtttg aaacaagaag
    88801 aaatcatggc gatcggtgat gaggaaaatg atttaccgat gatccaatat gctggactag
    88861 gcgtagcgat ggagaatgct gtgcctttcg tcaaagaagc agcagatttc gtgacagcca
    88921 gcaatgaaga aaatggcgta gccaaagtga tccaaaaatt tgtattagag tagggggagt
    88981 caaatgggat tttttgataa aatcaagcgt gcttttactg gcgaagagaa aacagaagaa
    89041 gttgaagtac aggagaagta cgacaagggt ttagaaaaga cgcgtaaaac atttggtgaa
    89101 cggatgaatg agttgtttgc caattttcga acagtcgatg aagacttttt tgaagaatta
    89161 gaagaaacac ttatcggtgc agatgtaggt tttgaaacag cgatacgaat caccgaagca
    89221 ttacgtcaag aagtgaaact cagaaatgcg aaaaaatcgg cagaagtcca aaatgcgatc
    89281 atcgagaaac ttgtggatct ctatgaagcg gaaggaatca atgaagtaaa tgaactaaat
    89341 atccaagaag acggactaac agttattttg tttgtcggtg ttaatggtgt agggaaaaca
    89401 acaagtattg gtaaacttgc ccatcagttc aaaaacgaag gtaaaaaagt attgatggca
    89461 gcagcagata ccttccgcgc tggagcaatc gaccagttag ttgtctgggg cgaacgcgct
    89521 ggtgttgaag ttgttcgtgg caatgcagga ggcgatccag cagctgtcgt attcgatgct
    89581 ttggaaagag caaagacaga gcaggcagat gttttactag ttgatactgc aggccgactg
    89641 caaaataaag tgaacttgat gaacgaatta gaaaaaataa aacgagtaat ccagcgggaa
    89701 ttaccagaag caccccaaga aacacttctt gtattagatg caaccactgg tcaaaacgcg
    89761 atggttcaag caaaacaatt caaagaaaca acagatgtga ccggtcttgt tttgacaaaa
    89821 ttagatggta cagcaaaggg cggtatcgta cttgctatcc gtaatgaatt gcatttacca
    89881 gtgaaattgg tcggtcttgg tgaaggaatc gatgacttgg agccgtttga tccaaatgat
    89941 tttgtcgttg gattgttcaa aggattacta aaagaagaat aacccatgtc taaaaagcag
    90001 gagctgtgac aaaagttgca gctccttgtt tgaatgattg tttttaacat agctgtgttt
    90061 ttgatgcctt catttttata cttatttgta agcggttctc aaacaatttc gttctttttc
    90121 ttagactttt tgattataat tagattcata tgaattggag gtcacttaat gatgaatttt
    90181 aagcaattat tattgcatgt caatgcgcgc ccttttatcg accaagctcg atttggtata
    90241 gaacgggaag gacagcgggt tgatcttgca ggaaatctag caaaaaccga tcatccagca
    90301 atctttggcg atcgatccta tcacccctat atccaaacag attttagcga aacacaaaca
    90361 gagatgatca cccctgttac cgattctatt cccgaattat ttcagtatct cgctgctgtt
    90421 tatgatgtga ctgctcgttc tataccgaaa gaagagatga tctggccatt aagtatgcca
    90481 cctgccttac cggaaaaaga cgaagagatc attattgcaa aattaaaaaa tttcgaagat
    90541 gtcttgtatc gacgttattt agcaaaaaaa tacgggaagc gaaaacagat ggtaagcggt
    90601 attcatttca attttgaatt tggcgatgag ttgctaagaa cattgttcag ccatcaggaa
    90661 gaatttcaag atttttccga attcaaaaca gaactttatt tgaaaacagc gagaaacttt
    90721 atgcgctatc gctggatgat cacttattta ttcggtgctt caccaatgag tgagaagaac
    90781 tactttttag atgaatcaca tccgcaagaa cctgttcgca gtattcgcaa cagtgcgctg
    90841 ggctatacga atcatccaaa tgtgaaagtt tcctatgctt ctatgaaaca gtatttagca
    90901 gatatcgagc gaatgatcga agaagggaaa ctttcggagg agaaagaatt ttatacaccg
    90961 cttcgtttcc gaggcggaaa gaaagtcgca gatttagcaa caacaggtgt tcgttatatt
    91021 gaattgcgga atatcgattt gaatccttat gcaagattgg gtatcaatcc agagcaagtt
    91081 cggttcttac aattattcct gatgtacatg ttatggacag aagaaaaaga agactgtgac
    91141 caatgggtag cagaaggtac gactcgaaat aacaaagtag cactagaaca accatcagat
    91201 caaacagaat ttcatcaaga aggtagagaa attcttgaag gaatgaagca aatgctggtt
    91261 gaattggatt ggttggattc tctttattta gtagaggaag cattgactca aatggatcat
    91321 cctgaacaaa cgttagcagc gaaactctat caagaagcac agctatcgag tcagcaagaa
    91381 gttgctgtgg cattgggaca tcaatattat aaagaaagcc atgaacgtcc ctatcagttg
    91441 gccggttttc gcgaaatgga actttctact cagatattta tgttcgatgc catccaaaaa
    91501 ggtgtccaag tcaaagtatt agatgagtcg gatcaatttt tgcgtctgca attccaagac
    91561 catgtagaat atgtgaaaaa tgcaaacatg acaagtaagg acagctatat cgtcccactt
    91621 atcatggaaa acaaaacagt aacgaaaaaa gtcttaaaag aagctgggtt ccgggtacca
    91681 ggaggcgcag aattttcatc catggaagaa gcggtaaaag cttatccgag atttgccgag
    91741 caggcatttg tgatcaaacc taaatcaacc aattacgggt taggtatcac gattttcaag
    91801 gaaggagcta gtctggaaga ttatcaagca ggtttagcca ttgcttttcg tgaagacagt
    91861 tcggttttag tagaagaatt tatgccggga acggaatatc gtttctttgt gatcgacgga
    91921 gaagtccaag ccatcatgtt gcgagtccct gctaatgtta ttggtgacag tatccgaaca
    91981 gtgaaagaac ttgttgaaga aaaaaatagt gaccctttgc gtggaacgaa tcatcgtgca
    92041 cctttagaat tgatccaatt aggtgaatta gaacaattga tgttgaaaga acaagggttg
    92101 acgatcgaat cagttcccca agccaatcaa attgtttatc taagggagaa ctcaaatatc
    92161 agtacaggtg gtgattcgat cgatatgact gatgaatttt ctgaagctta caaaaaaatc
    92221 gccgtttctg cggtagaagc actaggagct aagattagtg gtatcgattt gattatacca
    92281 gataaagaaa tcgatcctac aactgataaa aaagcatatg ggatcatcga agcaaatttc
    92341 aatccagcca tgcatatgca tgtttatcct tttgcaggaa aaggcagaag attgacgatg
    92401 aatgtattga aattgttata tcctgaagta ttttaaaaga aaaagctttt gacagattgt
    92461 cctatttgat gtcaatctga tcaaaagctt tttttgcatg taaaaattta tgtgtttttt
    92521 cgttttaaaa taaacgatcc cagaagcttt ttttcttttt tggtttttct ttttcagctg
    92581 gcgaagagtt ttcttctggg tatttttttt cgatatcaat cacaggttca tttactgctt
    92641 cttttgctgt taagagcaat ccgaattcct caggatttgc agtgacgtaa tcgttgacta
    92701 cagtgaaacg gctattgttt ttcgttgcta gctggatata ttggttttgg acagaaaggg
    92761 gtagagcccc attcaagagt atggaagcat ctggatgttt tttgagttct tttaaaaagt
    92821 tttctttgtt ttttggttgg atcatttcag caacagtcat actaagatag cagcgttccc
    92881 tgaatgtccc taaatattta tgctgttctt cagggttcac taaaggtgta ccataaagtc
    92941 ccttatccaa atggcttttt acatcatctt gtgccataaa aaataccctc cttcttctct
    93001 ttgtctttct tacgattata gcatattccc aataatacct aaaacatatg aaaaagcggt
    93061 tgcttttgtt caacgattca cttaaaataa gaatgaaaat cattctcaat tagaaaggtg
    93121 gattggatgc gctacggacg actagtagca ttattagtta ttttaagtag tatttcattg
    93181 ttcatcggag tatcgaatgt acaattgtca gaactgctcc agttagatgg gcaaagctta
    93241 caaatcttgc tagtgactcg tttgccacga acgatttgtt tgatccttgt gggagcgacc
    93301 agtagtattt gtgggttgat catgcaacat ttgacccaaa ataaatttgt ttcaccaacg
    93361 actgcgggca cgatggatag tgcgcgttta ggtattttag tggctatgat tttcctacct
    93421 ggtgcatcct tgattgtccg atccttgaca gcattttgct ttgcttttgc aggaacattg
    93481 ctttttttat cccttacgcg attgttcctc cagaaaaatc aattgatgct tccgttgatt
    93541 ggagtcatgt tagggaatat cattggttca gttgctactt tttttgctta tcaattccag
    93601 ttggtacaaa atatgacttc atggcttcaa gggaattttg cgactgtgat acgaggaaat
    93661 tatgagttat tgtatgctgt tgttccattg ttgctcttgg tttggctatt tgcttaccga
    93721 tttacgatca ttggtctggg cgaatcgttt gcagtaaatc tgggaataga ttaccagaag
    93781 atgcagtttt taggcattgg cttagtatca ttggctagtg cagtcatgtt gatcatggta
    93841 ggaagcattc cttttatcgg agtgatcatt ccgaatcttg tctctagaat atatggagat
    93901 caagtgcaca aaacaattgg cgtaactgct ttagcaggta gtttgttctt agtattttgc
    93961 gatcttgtcg ctcgcgtgtt gatttttcct tatgaaatac cagtcagtgt gatcgttgga
    94021 atcatcggtg gggctatctt cctttatctt ctgataaggg gacgacgata tgcttaaaaa
    94081 aaagccatct atttttattg gtatgcttgt tcttgtttta tttgttacag gaatgtactt
    94141 gacgatccat acatatggaa actggcattt tgcatgtgca ttgaggagta agaaaatttt
    94201 tgcatttatc cttgtctcgc tagctgtcag cagtgccaca atcagctttc agacaattac
    94261 gcataaccag tttttgactc cttcagtatt gggacttgag tcgttatatg tccttgtcca
    94321 aacggtcctt ttcttcttta tcggaggagt gacgatgctt cagcagacag gtacagtttt
    94381 tttcttattg aacgttgggc tgatggtcgg gctgagtcta ttgttatttt tctttctttt
    94441 gaaaaaaatg agagcgaatc tttttttatt actgatgtca gggatgattt taggtacgct
    94501 ttttggtagt gtcagtacct ttttacaagt catcatggat ccgaatgaat atgatttgct
    94561 tcaaggcaga ttatttgcaa gctttggcaa tatccagagt cagtatcttt tgcctgcttc
    94621 tttgatcatt atcggctgct ggtacctgtt atttcgatat cgacatgtgc tagacgtttt
    94681 ccatctcgga aatgaacaag cagtaaacct cgggatagac acagatcgtt ttgaattttt
    94741 gattttatgt tgtgtcagtc ttttgacagg gacatcaacg gctcttgtgg gtccagttac
    94801 ttttttaggg tttattgtcg caaatatcag ttatcgtgtc ttttctactt atcgtcatgg
    94861 tcagctattt atcggaagct ttctggtagc tatgctgttt ctgacaggag gacagctcgc
    94921 agtagagcag attttcggct ggaatacaac agtgagtgtc gtcgttgaat ttgtcggagg
    94981 aatttatttt gtcacaaaat tgattcgcga aaggaagagt atcctatgat tgatttacaa
    95041 aaggtcaata aactgtacca tggaaaatca gtggttaaag atgtacagtt aacgattcct
    95101 gaaaagaagt tgactgcttt tatcggtcca aatggtgctg gaaagtcaac ggtgttgtcg
    95161 atgatcagtc gtttgatacc aaaagataca ggtgaaatct atttagacca taacgaagta
    95221 aaggcttgga aatccaatga attagccaaa aaattatcga tcctacgaca atcgaatgaa
    95281 ataaatgtcc aaatcaccgt gaaagaatta gtagaatttg gacgatttcc ttatacgaaa
    95341 ggaaaattga cgacaaaaga cgaggaaatc gttcaggaat cactggaaca ccttgggtta
    95401 gaagaaatag cggatcaatc gattgacact ctttcaggcg gtcaactcca acgtgcgtat
    95461 attgcaatgg tgctagcgca agacacggat tatattttac tggatgaacc tttgaacaat
    95521 ctagatatga attttgcggt tcaaatcatg cagattttaa aaaatttagt acaagaaatc
    95581 gggaaaacaa ttgttattgt tcttcatgat atcaactttg ctgcaagtta tgcagacgaa
    95641 attgttgcaa tgaaagatgg acagttattt acgcaaggaa cgacccaaga aatgataaaa
    95701 aaagatgtat tggatgaatt atacgaaatg gacatacgga tttgtgagat tgaaggccgc
    95761 agattctgtc tttatttcta aaactaacat taggagagaa gagatgaaaa aattagtagt
    95821 aggtatcttg attctagcat catttggttt agcagcatgt ggaaatagca acaacaccag
    95881 tcaggcagac acaaaaaaaa gcagcacaca aactgaaact acattgacga taacagatag
    95941 taatggagac caaatcgagg tgccaaataa tccagaaaaa gtcgtcgtat tcgacaatgg
    96001 ttctttagat acgatggatg ctcttggtgt tggagataag gtagttggag cagctacaag
    96061 cagccttcct gagtatcttt catcctataa aaaagtagaa tcagcaggtg gaataaaaga
    96121 accagactta gaaaaaatta atcagcttca gccagatcta atcatcattt ctggccgaca
    96181 aagagatttt caaaaagatc taagtcaaat tgctccgacc attttccttt ctttagatgc
    96241 gaaaaatcca tgggaatctt tccagcaaaa tgtcactgct ttaggggaga tctttggtaa
    96301 gcaagaagaa gctaaaactc aattagagga attatctagt gcaatcgatc agacgaaaaa
    96361 gaaagcagaa gctactgaca aaaaagcact agtcacactt gtgaatgaag ggcaattatc
    96421 agcctacgga tctggttcta gattcggttt catccatgat ttatttggct ttgaacaagc
    96481 agatgatcaa atcgaagcat ccactcacgg tcaaagtgtt tcttatgaat atgtgctgga
    96541 aaaaaatccg gatatcttat ttgtagtaga cagaacaaaa gctattggtg gagacgacag
    96601 caaagatgat atttctgcca atgaactggt agcacaaacg aatgctggta aaaatcagca
    96661 gatcatctcc cttgaaccag atgtctggta tttaagcggg ggcggtttag aatcaatgaa
    96721 actgatgatc gaagatgtga atcaagcatt taaatagcat aaataacaga ggattaaact
    96781 ctcatactat gtaacaataa tgtttcttac attcttaaat agcaagagcc tatcctcaaa
    96841 gaagttatca aaaaccatcc gtaaaatcgt attgatgatt ttacggatgg tttttattta
    96901 tttgagaaat cttttttcaa cgtgaattca ttatctacat catacgggtt gattctagaa
    96961 tggggagtgg caaaaaatag tgtgatccat tttttcagca tcaagatcgc ctcctttgat
    97021 ttgaatcaaa ggtacaaagc gaaacttaga taaaacttaa atttagatat ttttattttg
    97081 taccgatctc ttctttaaac gatgttccat ctgctgcttc tcggcaataa gtccactaga
    97141 ctagaaaata tgagaagcta ttttctgtcc agcgttctta ttactcaaaa caaatcgcag
    97201 ttgtcacaat ctcttctaaa cgatgttcca tctgctgctt ctcggcaata agcccactag
    97261 actagaaaat atgagaagct attttctgtc cagcgttctt attactcaaa gcaaatcgca
    97321 gttgtcacaa tctcttctaa acaatgttca atcagctgac cttcggtatt agctcaaagc
    97381 actgatttca caacctctat ttaggcatgt gtctcagcaa gcggttgata gttgatgatc
    97441 tgcatttttc gataaaaatc ggcgggaagc ttactccacc gttttagtaa ttgatagaaa
    97501 taactctctt ctgctttgat tttatactcg cttaatatca atccaaaact attcgttttt
    97561 tttgaagcta attttttata aatatccaat gctttggttt tatcttcatt gacaaagagt
    97621 gtttttactt caatataaga accctcgata ttttgtgttt cttcgataaa atagatgttt
    97681 tttatatatt ccttcatagt gattcctcca aagaagtctt ttatttgata agtttactct
    97741 accgaagagt tgttaataat ccgtgaacaa aaagtaattc tttttccaat agttgttaaa
    97801 ggttttttat tttttatagt attttttgtt atttaagaaa ggaataaggt taaacaaata
    97861 acaggttgct ttttgggtct ttttccgttc aagaaactga acgaagaaaa gggaaagttg
    97921 tctcttttgt gataagagaa atcataataa acgaatgact tactttccaa taaattgaga
    97981 acagtcagaa ccagacaaat tagttgcatc tgatgaataa agtgattatt ctatgacatg
    98041 aggtgataca gatgaaatta gaagtgacaa aagaagcaca agaagtattg aagaataaat
    98101 tagaagcaga cgatcaactt ttattagata tcgaaaacgg tgacggtcca tttgcagaaa
    98161 gccaagtcag ttgccaattg gatacggcat ttcgattgat cattgtcaaa aaagatacac
    98221 aaacagatct aagtctttat tctgtagtgg ctgatacacc agttggacca atcaaaatca
    98281 aagacagtgc aatactttac atggatgatc catccacact tgctttagaa ccaacttata
    98341 atagtttaca attaaaagga ccaagcggct tgagaaaagg caacttgcaa gttgttcgaa
    98401 aagactagag ggataaaatg ggaaaattgg cgatcatcag cgacctacat gtggacatca
    98461 ataaattcgg cgaatcagag ctgatgcagc tttggcaagt tttgcaaaag cacaagatca
    98521 ctcggcttca tctagccgga gatacggcta ataaagtaga ccgttgccta gctgttgtcg
    98581 aatttttcgt caataaagga ttatctacga catttcattt cggcaatcac gagttggcag
    98641 atgtgcccaa tgaacagatg atggaccatt ttccagaccc acatttttta aatgaacggt
    98701 atattgcttt aaatgagcag accgttcttt tagggatgaa tggctggtac gactatcagt
    98761 tttccgaatt gaaagattac gaggcaatcg tacgaatgaa gcgtttgtat tggtatgatc
    98821 ggttcatcca gcgtgggaaa acagatctcg aggtcaatgc agaaatgctt gcagctgcaa
    98881 aaaatttgct agaccagctg gaagcaaaaa aacttgaggt gattttatcc actcatttcg
    98941 ttcctaaacg ggcatttatc atgtatcaat ctgctcctta tgagcgttgg aacaaattaa
    99001 atgcattttt aggatcagaa tcattcggag aattacttga ccgttattca aatatcaaac
    99061 aagtagtttt tggccatacg catcgtcgct ttgaagataa atccatcggt cacacgattt
    99121 acagctgcag accttttggt tattattatg aatggcagct gacccgagat tttgtcttga
    99181 aagaacagtt gctagacagc tatgatccta tgaaattacg tgtcctcttg cgtgcccatc
    99241 acccagcttt tcaggcctat caaacacaac atttgcaaga ggaatttgaa caagcgatga
    99301 caatcgttcc gtattgagac agcattagta atgtgtaaaa gaaaatacct tgtaaaacat
    99361 aaggataagt aagagcagta gatccattta tcgtggatat gctgcttttt ttgcggaaaa
    99421 taatcagcga atgtttattt gatcggatga tttgtaactc gcagagtact agatgcaagc
    99481 gtgtattctt tttttgaacg ataatcacaa aaaaaaatat aatatacatt ttttatttaa
    99541 ttataattaa tattgattga agtctaggca gtcaagtcac attttgatag ataggagggg
    99601 gacttacgaa ttattttaaa ggctatgaca gctgtatttc tagattgtgt ttgcatcaag
    99661 ttgcaataaa aactagaaat caaatcgggc tgataatcat ttataactaa tcgatacata
    99721 ggaccaaata taggtctatg ttcttttatt tcataagtat cgtaaaacag aattttgcta
    99781 tggattaatc caatcgactg atatgtttag taaataaaga aagaggaatg tcaatgatga
    99841 aaaaagaaat cgtattaaca gcagcaatga tgttattcag catggtagca agtacgactt
    99901 ttgtttctgc aactgaagta tacccaaagg aatataacac agaaggaacg attacatttg
    99961 aagcaggaga tgagggtgta actcctcctg ttgatccaga gaatcctgat cccaataaac
   100021 ctgtcgatcc atcagatcct ccaagccctg gaacaggagg ggcattatcg attgattatg
   100081 gatcaaaatt caaatttggg actcaaaaaa tttctactgc ggataaaacg tattatgcag
   100141 ctgctgatgt gatgaacgat gggtctagaa aaccgacata tgtccaagtg acagatagga
   100201 gaagtacctt atctgggtgg aaattaagtg tctctcaacc agaacagttt aaaactgcca
   100261 gtggggatga actagttgga gcacagctga aatttacgaa agggcaggct gtttctttgg
   100321 ttgatccaac atatacgccg caaacggtga attctgaatt aacgttgaca ccgggaggaa
   100381 acaatacgtt agcaattaat gctaaatcag gaacaggtgt ggggacttgg gtctatcgat
   100441 ttggtgcaaa tgaaaatgaa aatcaagatg cggtacagct ttcggtaccc ggtaaatctg
   100501 tcaagttagc gcaacaatat tccactaaat tagtttggac attagaagat acacctaaca
   100561 actagtttac tgaaaagaac tgttaaaaat aaatacgtag tcatattgtc ttgcgaggta
   100621 atcgagcaaa aattgtcaaa ggctaggcta aatttgtttg gatacgaata gttcatctag
   100681 gaatttaacg atcaggagga aaagatatat tgaaaaagag gaggatcgtg ctcattttaa
   100741 gcagtctctt agtgactaat atctttccga tcatagggta ctctttggaa ttgaatcagt
   100801 tggagcatgg aaatagaaga gacgaagaaa gcaaactcac ttcgacattc gacagtttaa
   100861 tagaatccaa ccagacacaa gaaagcagtg aggatttaag tatttcgagt agtggagaga
   100921 ccactgacaa cgtagaagct gagggttcgg acaaacaagg actagaagat gcaccaattt
   100981 ctgcggttga gctgcctgac gatcccaata ttatttctat cgataaaatt tttcaaacac
   101041 ctatcggtac tagcacgtcc atattggaaa atgggaagtt actgcagttg aacccagcag
   101101 aaaggtctca aagaggagct atttggtcaa ataagaagat tagtttactc tcggatttta
   101161 catttaaatc ttatttatac ttaggaaatc aacgagatag agcgggagat ggcatgacct
   101221 ttacattgac tggcgatcct agaatggcta gtaatcccag tcaagttatt ggatcgccag
   101281 gtatgggaat tggctcatat tctactcgtt ctggccaacc ttacattgcg aatgcgctct
   101341 cgattgagtt tgatacgtat aaaaatagtg gttcttctga tcgaatggat cgagagataa
   101401 ctgctgataa tagagggcat ggacatgtcg cttttgtcac gcctaaacca aataataata
   101461 attattctgg agagcatact ggcgtgacgg tagctcctac atttctttct aatggcacat
   101521 ggcgcttgtt aataattaaa tgggacgcgg agcaaagaag tcttacttat gatttggaag
   101581 gtgtcggtcg ctcttctcgg attatacctg atctcattag gcagtttggt tctacagaag
   101641 taaattgggg gtttacttct tcaacaggta ctttttttca agaaaatgct gtcgctgtca
   101701 cgcgacttcc atcgaatgtc acatcaacag ctgatgtaaa agtcaacgat agtgattatg
   101761 gggccatcgc tgaagctgga ggaaacgata agataactat cagaaataca ttgaaggtcg
   101821 agggggattt tcttgaatat aagaaaatgc agactagtat cgacctccca caagaattaa
   101881 ctattcccga gcatatcttg atcaatggaa atcaaattga tgccaaagat atcttgataa
   101941 ccggaaatcg agtcacgcta aatctgacag attattttat tgctggagat acgttaataa
   102001 tcgaattgga gacaactttg caggaagtaa ttcctgaaaa agtactcact atgcattttg
   102061 aataccttga aaacgggaat ttgattgagc aatcgaatga aataaaccta aagatcgcaa
   102121 agctgaagga gatagatatc catgtttatt ataaagataa agacagtcag attgatattg
   102181 ctgaaatgaa aacaatcact gggaaaatag gagagaagta taaagaaact cctattgata
   102241 ttgaagggta tgtttttgac tcggattcag cgaatacaga aggcatcttc tccgaaaata
   102301 cagaggatat ctatttttat tacagaaaag gcaaacttta tctctcaact gctcctcaat
   102361 atgtcaattt tggaaaacat aaaatatcca gtagaccatt aagtgaattc ggacagtttt
   102421 caggtgggtt gaaaattata gatgaacggg cttttggaag ttggaaatta caattgaaac
   102481 aagagaaagt tcttacaaat ggagattttg agcttccaga agctctttcc tttgttacct
   102541 ctgaaaatac aacggttata gggacatccg ctgttacgat tttcgaaagt gatcaaaagg
   102601 gtgaaagcga tttgtcctct cttttagatt ctacagctca tcgtggaatc agaataaata
   102661 ttcctgtaga aaaccaaaga atcggaacct ttgaggggaa actatcttgg atacttgcag
   102721 atgtcccagg aaattaacag gaagagaaga atataacaaa attattgagt ttcaagaagg
   102781 gcacaggtac cataggctct tgtgtcccac agagacaaga ttttgttata gtcttctctt
   102841 acatatgatt aaaggagcga ttttcttgag accaaaacaa caattattta taacactcct
   102901 agttatttta ggggcacttt ttaacaatat atcagtgtca gctagtgcat ttaatttttc
   102961 agtgacgccg ataacttcag agaatcaaat cgacaagaga aaaacttact tcgatcttca
   103021 acttgatccg gatcaggaag tagaagtaaa agcggaattg agaaatgata cagaaaaaga
   103081 agtgaaaatc gatatttctg tgaatagcgc aaccaccaat tcaaatgtga tggttgaata
   103141 cggaaaaaac gagattgaaa aagacatgag cctgatcttt gatctggtag attatgtgag
   103201 ctatccacag atagtgacgc taaaacctaa aagtgtccaa accgttgctt ttcatgtcag
   103261 gatgccgaat gagcgatttg atggtgtatt ggctggtggg atcacattcc aagaaatcca
   103321 gacggaaaag gaccaaacag aaactaagga tcaaagctta tcgattgaga atgaatacac
   103381 ctatattatt gctttgttga tgcaacagac actgaacgaa gttgctccga accttactct
   103441 tcatgaagtt aaaccagatc aaatcaatgc aagaaatgtc atcttagcaa acgttcaaaa
   103501 tgatcaaaaa acctatatca atcaagtagt tattgaaaca aaaattacaa aaaaaggaca
   103561 ttctgaagtt ttatatcaag aggaaaaaga aggattgcag attgcgccga atacaaattt
   103621 ttcttttcca actgctttaa atggtcaacc gctaacccca ggtgaatatc accttacaat
   103681 gactgtttta ggaaatgaaa atgaaaatgg aaaattttcg agaaaaaaag gaaatactac
   103741 gatcaactat accaatcaat gggtatttga aaaagatttt acaatagacg gaaaagtagc
   103801 caaggaattg aacacgacgg atgttacgat taaaaaagac aataattgga tttatatctt
   103861 agtagcttca cttttcttat tgtttatttt attatgcata cttttgtttg ttttgagaaa
   103921 aaaagaagag aaagaaaagt aaggttaaga taacaaatag taaattttta ttattgtagg
   103981 atggttgggc tctttttata acaatataaa atataccatt ttattaataa aaatgcatac
   104041 tagtaaatga tataattcta ataacaggtt tgatcgcgag aaaggccaaa taaaacgctc
   104101 tccaaaatgt taaaaaaaag acaggacgat gtatattata caaacaagaa tcgctgacaa
   104161 cagtgactcg gatccaggaa atcagtttta tccccagcta gttggtgaat gcatggaaag
   104221 taatatacgt agaaagagct gatgactaat gaattatgag ttttttttaa gcaaagaaga
   104281 tcacagaagt tattcattac ttaaatattt agaagcaagc tcaaatttat cagagtcaat
   104341 atcgaatgtt caagaagagt tgtcgctatc gacatttctc ttgaaaaaaa cgatcgataa
   104401 gctgaaacat gatttacaaa agttcgatct agagaaaaat ttgcgagtaa ccgtcagtga
   104461 attggatata tgtctggaaa ttaatggaaa atgttctagc aaagctctgt tatctcgtta
   104521 tataacggat tctttatcta tgaaaatggt attggctttc tttcaggaaa aatatatctc
   104581 agtagaaaat tttgctaaac agcatcacgt gagttattct gtggcttata aagtattaca
   104641 aagactaaaa agaaatctaa aaaaatatca aatttttttt gaaaagagaa agttga
//
LOCUS       DACGLW010000003       101336 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.003, whole genome shotgun sequence.
ACCESSION   DACGLW010000003 DACGLW010000000
VERSION     DACGLW010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 101336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 101336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..101336
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.003"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            complement(75..1442)
                     /gene="nhaC"
                     /locus_tag="IXT53_000253"
     CDS             complement(75..1442)
                     /gene="nhaC"
                     /locus_tag="IXT53_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAQ4904116.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(1551..2972)
                     /locus_tag="IXT53_000254"
     CDS             complement(1551..2972)
                     /locus_tag="IXT53_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ4904117.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(3215..5092)
                     /gene="tdc"
                     /locus_tag="IXT53_000255"
     CDS             complement(3215..5092)
                     /gene="tdc"
                     /locus_tag="IXT53_000255"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAQ4904118.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(5383..6639)
                     /locus_tag="IXT53_000256"
     CDS             complement(5383..6639)
                     /locus_tag="IXT53_000256"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HAQ4904119.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     gene            7219..8520
                     /locus_tag="IXT53_000257"
     CDS             7219..8520
                     /locus_tag="IXT53_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAQ4904120.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(8589..9188)
                     /locus_tag="IXT53_000258"
     CDS             complement(8589..9188)
                     /locus_tag="IXT53_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ4904121.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            9443..10324
                     /locus_tag="IXT53_000259"
     CDS             9443..10324
                     /locus_tag="IXT53_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4904122.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            10337..11947
                     /locus_tag="IXT53_000260"
     CDS             10337..11947
                     /locus_tag="IXT53_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HAQ4904123.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAKGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(12024..12683)
                     /locus_tag="IXT53_000261"
     CDS             complement(12024..12683)
                     /locus_tag="IXT53_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ4904124.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(12966..13631)
                     /locus_tag="IXT53_000262"
     CDS             complement(12966..13631)
                     /locus_tag="IXT53_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4904125.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(13778..14383)
                     /gene="dhaL"
                     /locus_tag="IXT53_000263"
     CDS             complement(13778..14383)
                     /gene="dhaL"
                     /locus_tag="IXT53_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HAQ4904126.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(14411..15394)
                     /gene="dhaK"
                     /locus_tag="IXT53_000264"
     CDS             complement(14411..15394)
                     /gene="dhaK"
                     /locus_tag="IXT53_000264"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HAQ4904127.1"
                     /translation="MKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRVVAKNQKAEQ
                     VGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEADSGKGVLLIV
                     KNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAGTVLVHKIVG
                     DAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDEIEYGVGIHG
                     EPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQFVFMNDVLA
                     LLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTISW"
     gene            complement(15396..15791)
                     /gene="dhaM"
                     /locus_tag="IXT53_000265"
     CDS             complement(15396..15791)
                     /gene="dhaM"
                     /locus_tag="IXT53_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HAQ4904128.1"
                     /translation="MKKSILLISHSQKVTDGIKEMIEQMQKSEEVQIFSLGGINGEIG
                     SDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEEEQQQRYHLVDAPLVEGA
                     FAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            15945..16265
                     /locus_tag="IXT53_000266"
     CDS             15945..16265
                     /locus_tag="IXT53_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HAQ4904129.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(16377..17246)
                     /locus_tag="IXT53_000267"
     CDS             complement(16377..17246)
                     /locus_tag="IXT53_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ4904130.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(17342..18646)
                     /locus_tag="IXT53_000268"
     CDS             complement(17342..18646)
                     /locus_tag="IXT53_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ4904131.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(18643..19152)
                     /locus_tag="IXT53_000269"
     CDS             complement(18643..19152)
                     /locus_tag="IXT53_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4904132.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(19327..21462)
                     /locus_tag="IXT53_000270"
     CDS             complement(19327..21462)
                     /locus_tag="IXT53_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ4904133.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(21545..23200)
                     /locus_tag="IXT53_000271"
     CDS             complement(21545..23200)
                     /locus_tag="IXT53_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ4904134.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(23202..23516)
                     /locus_tag="IXT53_000272"
     CDS             complement(23202..23516)
                     /locus_tag="IXT53_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ4904135.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            23686..23946
                     /locus_tag="IXT53_000273"
     CDS             23686..23946
                     /locus_tag="IXT53_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HAQ4904136.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            23960..24274
                     /locus_tag="IXT53_000274"
     CDS             23960..24274
                     /locus_tag="IXT53_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur reduction protein DsrE"
                     /protein_id="HAQ4904137.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(25004..25558)
                     /locus_tag="IXT53_000275"
     CDS             complement(25004..25558)
                     /locus_tag="IXT53_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HAQ4904138.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(25684..27613)
                     /locus_tag="IXT53_000276"
                     /pseudo
     CDS             complement(25684..27613)
                     /locus_tag="IXT53_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(27919..28635)
                     /locus_tag="IXT53_000277"
     CDS             complement(27919..28635)
                     /locus_tag="IXT53_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAQ4904139.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(28636..30459)
                     /gene="glpO"
                     /locus_tag="IXT53_000278"
     CDS             complement(28636..30459)
                     /gene="glpO"
                     /locus_tag="IXT53_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HAQ4904140.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(30473..31969)
                     /gene="glpK"
                     /locus_tag="IXT53_000279"
     CDS             complement(30473..31969)
                     /gene="glpK"
                     /locus_tag="IXT53_000279"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HAQ4904141.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(32105..33565)
                     /locus_tag="IXT53_000280"
     CDS             complement(32105..33565)
                     /locus_tag="IXT53_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ4904142.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(33555..34850)
                     /locus_tag="IXT53_000281"
     CDS             complement(33555..34850)
                     /locus_tag="IXT53_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ4904143.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(35182..35859)
                     /locus_tag="IXT53_000282"
     CDS             complement(35182..35859)
                     /locus_tag="IXT53_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HAQ4904144.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(35889..36980)
                     /locus_tag="IXT53_000283"
     CDS             complement(35889..36980)
                     /locus_tag="IXT53_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HAQ4904145.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(37081..37515)
                     /locus_tag="IXT53_000284"
     CDS             complement(37081..37515)
                     /locus_tag="IXT53_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ4904146.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(38099..39556)
                     /gene="ascB"
                     /locus_tag="IXT53_000285"
     CDS             complement(38099..39556)
                     /gene="ascB"
                     /locus_tag="IXT53_000285"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ4904147.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(39553..41448)
                     /locus_tag="IXT53_000286"
     CDS             complement(39553..41448)
                     /locus_tag="IXT53_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ4904148.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(41506..43281)
                     /locus_tag="IXT53_000287"
     CDS             complement(41506..43281)
                     /locus_tag="IXT53_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ4904149.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(43331..44827)
                     /locus_tag="IXT53_000288"
     CDS             complement(43331..44827)
                     /locus_tag="IXT53_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ4904150.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(45021..46643)
                     /locus_tag="IXT53_000289"
     CDS             complement(45021..46643)
                     /locus_tag="IXT53_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAQ4904151.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(46971..48284)
                     /locus_tag="IXT53_000290"
     CDS             complement(46971..48284)
                     /locus_tag="IXT53_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAQ4904152.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(48529..48753)
                     /locus_tag="IXT53_000291"
     CDS             complement(48529..48753)
                     /locus_tag="IXT53_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4904153.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(48773..49141)
                     /locus_tag="IXT53_000292"
     CDS             complement(48773..49141)
                     /locus_tag="IXT53_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ4904154.1"
                     /translation="MIQKTRVMLYVNDVEMICRFFVEKIGAEISETIELPEEFKSIVL
                     SISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETIYENMEEPGEITDNNGILT
                     FNFSDPEGNYFVIGKAESSD"
     gene            complement(49337..50047)
                     /locus_tag="IXT53_000293"
     CDS             complement(49337..50047)
                     /locus_tag="IXT53_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4904155.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(50065..50562)
                     /locus_tag="IXT53_000294"
     CDS             complement(50065..50562)
                     /locus_tag="IXT53_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ4904156.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(50599..51051)
                     /locus_tag="IXT53_000295"
     CDS             complement(50599..51051)
                     /locus_tag="IXT53_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003331879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ4904157.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(51186..51791)
                     /locus_tag="IXT53_000296"
     CDS             complement(51186..51791)
                     /locus_tag="IXT53_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAQ4904158.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(52241..52861)
                     /locus_tag="IXT53_000297"
     CDS             complement(52241..52861)
                     /locus_tag="IXT53_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ4904159.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(52863..53732)
                     /locus_tag="IXT53_000298"
     CDS             complement(52863..53732)
                     /locus_tag="IXT53_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HAQ4904160.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(53755..54162)
                     /locus_tag="IXT53_000299"
     CDS             complement(53755..54162)
                     /locus_tag="IXT53_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAQ4904161.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(54181..55470)
                     /locus_tag="IXT53_000300"
     CDS             complement(54181..55470)
                     /locus_tag="IXT53_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ4904162.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFNNATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(55497..55799)
                     /locus_tag="IXT53_000301"
     CDS             complement(55497..55799)
                     /locus_tag="IXT53_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ4904163.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(55824..57917)
                     /locus_tag="IXT53_000302"
     CDS             complement(55824..57917)
                     /locus_tag="IXT53_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ4904164.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(58406..59170)
                     /locus_tag="IXT53_000303"
     CDS             complement(58406..59170)
                     /locus_tag="IXT53_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HAQ4904165.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(59281..60150)
                     /gene="mdcB"
                     /locus_tag="IXT53_000304"
     CDS             complement(59281..60150)
                     /gene="mdcB"
                     /locus_tag="IXT53_000304"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HAQ4904166.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(60175..61251)
                     /locus_tag="IXT53_000305"
     CDS             complement(60175..61251)
                     /locus_tag="IXT53_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HAQ4904167.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(61275..61868)
                     /locus_tag="IXT53_000306"
     CDS             complement(61275..61868)
                     /locus_tag="IXT53_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HAQ4904168.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(61872..63548)
                     /gene="mdcD"
                     /locus_tag="IXT53_000307"
     CDS             complement(61872..63548)
                     /gene="mdcD"
                     /locus_tag="IXT53_000307"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HAQ4904169.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(63538..63837)
                     /locus_tag="IXT53_000308"
     CDS             complement(63538..63837)
                     /locus_tag="IXT53_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HAQ4904170.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(63939..65585)
                     /gene="mdcA"
                     /locus_tag="IXT53_000309"
     CDS             complement(63939..65585)
                     /gene="mdcA"
                     /locus_tag="IXT53_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HAQ4904171.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(65592..66503)
                     /locus_tag="IXT53_000310"
     CDS             complement(65592..66503)
                     /locus_tag="IXT53_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAQ4904172.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            66623..67480
                     /locus_tag="IXT53_000311"
     CDS             66623..67480
                     /locus_tag="IXT53_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ4904173.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(67667..68029)
                     /locus_tag="IXT53_000312"
     CDS             complement(67667..68029)
                     /locus_tag="IXT53_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4904174.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            68375..69814
                     /locus_tag="IXT53_000313"
     CDS             68375..69814
                     /locus_tag="IXT53_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ4904175.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(69821..72373)
                     /locus_tag="IXT53_000314"
     CDS             complement(69821..72373)
                     /locus_tag="IXT53_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HAQ4904176.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMGFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(72487..73818)
                     /locus_tag="IXT53_000315"
     CDS             complement(72487..73818)
                     /locus_tag="IXT53_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ4904177.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(73784..75262)
                     /locus_tag="IXT53_000316"
     CDS             complement(73784..75262)
                     /locus_tag="IXT53_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ4904178.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(75567..77402)
                     /gene="tdc"
                     /locus_tag="IXT53_000317"
     CDS             complement(75567..77402)
                     /gene="tdc"
                     /locus_tag="IXT53_000317"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HAQ4904179.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            77872..79299
                     /locus_tag="IXT53_000318"
     CDS             77872..79299
                     /locus_tag="IXT53_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ4904180.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(79391..79756)
                     /locus_tag="IXT53_000319"
     CDS             complement(79391..79756)
                     /locus_tag="IXT53_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAQ4904181.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(79753..80118)
                     /locus_tag="IXT53_000320"
     CDS             complement(79753..80118)
                     /locus_tag="IXT53_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HAQ4904182.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(80310..80558)
                     /locus_tag="IXT53_000321"
     CDS             complement(80310..80558)
                     /locus_tag="IXT53_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4904183.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            80557..80685
                     /locus_tag="IXT53_000322"
     CDS             80557..80685
                     /locus_tag="IXT53_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HAQ4904184.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            80742..82436
                     /locus_tag="IXT53_000323"
     CDS             80742..82436
                     /locus_tag="IXT53_000323"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HAQ4904185.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            82440..82559
                     /locus_tag="IXT53_000324"
                     /pseudo
     CDS             82440..82559
                     /locus_tag="IXT53_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            82641..82832
                     /locus_tag="IXT53_000325"
     CDS             82641..82832
                     /locus_tag="IXT53_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4904186.1"
                     /translation="MDLLPQMQAFEHHLPRGYEKAALHQVIRAAKLKQLLMFLTWLDL
                     LPQMQAFEHRLPRGYEKGV"
     gene            83210..84343
                     /locus_tag="IXT53_000326"
     CDS             83210..84343
                     /locus_tag="IXT53_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HAQ4904187.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            84374..84694
                     /locus_tag="IXT53_000327"
     CDS             84374..84694
                     /locus_tag="IXT53_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4904188.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(84723..85187)
                     /locus_tag="IXT53_000328"
     CDS             complement(84723..85187)
                     /locus_tag="IXT53_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ4904189.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(85318..86571)
                     /locus_tag="IXT53_000329"
     CDS             complement(85318..86571)
                     /locus_tag="IXT53_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ4904190.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(86600..86917)
                     /locus_tag="IXT53_000330"
     CDS             complement(86600..86917)
                     /locus_tag="IXT53_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ4904191.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(86943..87263)
                     /locus_tag="IXT53_000331"
     CDS             complement(86943..87263)
                     /locus_tag="IXT53_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ4904192.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(87727..89142)
                     /locus_tag="IXT53_000332"
     CDS             complement(87727..89142)
                     /locus_tag="IXT53_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ4904193.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(89159..90475)
                     /locus_tag="IXT53_000333"
     CDS             complement(89159..90475)
                     /locus_tag="IXT53_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ4904194.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(90625..91323)
                     /locus_tag="IXT53_000334"
     CDS             complement(90625..91323)
                     /locus_tag="IXT53_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HAQ4904195.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(91612..92605)
                     /locus_tag="IXT53_000335"
                     /pseudo
     CDS             complement(91612..92605)
                     /locus_tag="IXT53_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328323.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
     gene            complement(92686..93519)
                     /gene="msrA"
                     /locus_tag="IXT53_000336"
     CDS             complement(92686..93519)
                     /gene="msrA"
                     /locus_tag="IXT53_000336"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ4904196.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDTRENISVKEQ
                     LSKLEVTLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQDERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(93753..94496)
                     /gene="truA"
                     /locus_tag="IXT53_000337"
     CDS             complement(93753..94496)
                     /gene="truA"
                     /locus_tag="IXT53_000337"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HAQ4904197.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYRS"
     gene            complement(94687..95322)
                     /locus_tag="IXT53_000338"
     CDS             complement(94687..95322)
                     /locus_tag="IXT53_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAQ4904198.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(95469..96710)
                     /gene="sstT"
                     /locus_tag="IXT53_000339"
     CDS             complement(95469..96710)
                     /gene="sstT"
                     /locus_tag="IXT53_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HAQ4904199.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEPT
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            complement(97031..97708)
                     /locus_tag="IXT53_000340"
     CDS             complement(97031..97708)
                     /locus_tag="IXT53_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HAQ4904200.1"
                     /translation="MGLAFLLAGCTNGNESKKESTKETAKATIATTTTTSTESKPTAA
                     STETADTSTSSESSKTTETTQTSTSSSSVEASSSESSEQVEQTLWNADKSAQLEAFMA
                     TWGQTMGQQYKSYTNQMSVDLYGLKVPQVILNGEWKMAIGGVPVSAEWSESGTGQADY
                     QITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFNETANNELKNG
                     FNQIVYG"
     gene            complement(97959..98501)
                     /locus_tag="IXT53_000341"
     CDS             complement(97959..98501)
                     /locus_tag="IXT53_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ4904201.1"
                     /translation="MNIVEEKFAQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLKQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(98506..98733)
                     /locus_tag="IXT53_000342"
     CDS             complement(98506..98733)
                     /locus_tag="IXT53_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper resistance protein CopZ"
                     /protein_id="HAQ4904202.1"
                     /translation="MKKMIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSTKVKEA"
     gene            complement(98812..100692)
                     /locus_tag="IXT53_000343"
     CDS             complement(98812..100692)
                     /locus_tag="IXT53_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAQ4904203.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIIIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDNVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDQFSQYYTPTVLLIALLVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQRGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAMLKQAGAKHLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLH
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSSFGSLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLIGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            100978..101313
                     /locus_tag="IXT53_000344"
     CDS             100978..101313
                     /locus_tag="IXT53_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ4904204.1"
                     /translation="MGVEAVDKYLYLLAGNKIQKSLMDFIQELECTFHKKFTHSILLK
                     LLIHTACLIERTLINGHELKIISEDDTRPSHETIFHVKKAFKNIETEFGITVSYDECF
                     FIYDIIASK"
BASE COUNT        29623 a        21342 c        17319 g        33052 t
ORIGIN      
        1 aaaagcaatt ttcttgagtt tcttcttatt tcttggagtt aaacgcttct gtcccagcct
       61 cttttttcct tccttcaatt ttttacacgt cgacgattca ataaaaaacc gcctaatatt
      121 gtaaagacag gtgcaagaat cgagaaaaag gcgaatggca agtaatgcaa cgcgctaact
      181 ttcatcgttc ccataatgaa tgtgccgctg actccccacg gtacgagaga attgacggtc
      241 gcacctgaat ccgctagtgt tcgtgtcaaa taggtttttg ataagccaac gtcatcaaaa
      301 gaacgcttga atgtttctcc tggaagaatg atggacagat actgttctcc aacgatcaga
      361 ttgattccga tacagctcaa ggcagtaaac ccgactaaac gagaaggtcg gtccatcttt
      421 tcttttaatt cttgaacgat ggtatcaacg atcagatatt tgatcagtaa cccacctaat
      481 gccaatgcaa gtaaaatcaa actagcagaa cctaacatac tcatgatccc gcctcgagaa
      541 agcagttcat ctagctttgg atcaggtgta tttgcgacat aaccattcat caagatgtct
      601 gctgcttttc cgaccgtcaa acttttctca tggataaaag aaagaacgag agcagctgtc
      661 gatcccgcta gtaatgtagg gatagccgga atttttttga ttgccccccc aaataaaata
      721 actagtggaa tcaatgtcca aggagagatc caaaagctcg ctttcatgtt ttggatcatt
      781 tcattgatac tagctagatc ggctgaatga ctgttatgtc ctaatatcgt ataaaaaatc
      841 aacgaaagga cgaatgctgg gaaatccgta taacgcatac cagcgatatg ttcgaataga
      901 ttaactcctc caatacccgc tgccaagttg gtcgtatctg ataaaggcga gcagttattc
      961 cctaaaaagg caccggaaac gactgcgcct gtagtcattg catcattgaa tccaagtaaa
     1021 tgaccgatcc ccaaaaaagc aattccaatc gtcgaaatcg ttgtaaatga actgccgaca
     1081 gtcactccga caattgcaca aacaagaaat gctgtcggta aaaagaaacg cacagaaaca
     1141 agtttaaatc cataaaccat aatcgtaggg atggttccag ataaaatcca tgaagcaact
     1201 aagacaccaa tcaaaagaaa aatcaggatt ggaatgatcc ctggagtgat tcctttgatg
     1261 atcccgtcat ggatctcatc ccatgtgaac tttttaaatc tgccataaaa caacaaacac
     1321 atgaaagctg caaggatcgg gatatgtggc gagagctgga atccgatgat cagtactcct
     1381 aggagtgcca acaacacgat caatacagcg atactttctt ttaaagaaat atttttattc
     1441 atcgtcgatc tcctatttca aatgaaaagg ccctgccatg aaaaaactat tcacttggca
     1501 ggacctccta ctaaatcagc gacaaaacgt cggctgaaag acttattttt ttatttttct
     1561 tctaaacttt tttctacctc aatcccccaa gaatctttct tgaagtgatt gatgatcaat
     1621 ggaataacga acatgaccac tgtgatagct gctactaata atgcatcact caatgtcgct
     1681 gtacctaatg tagcaaatac gacaacgatg atagagaaga tcagcatgca gctgactacc
     1741 caagctaatc cgtttccgtt tttaccaatt cggtaaggac gttcgaattc tggatgtttc
     1801 ttacgcattt taagcaatga aacagcaatc aaaatgtaca cgatacaata aataacagtt
     1861 gtagcatttg taagtgtcaa gaatacagca cttacgtcat ccattacacc ataaagcaaa
     1921 gcaaataatg aaataacgat tgattgtgtt agaacgacat ttctagatac gccgtatttg
     1981 ttttctttat ggaatccgaa ttttggtgga aggaatcctt cacgagctac ttggatgatt
     2041 gtttttgatg gtccagtaac ccaagctgat aattgtaaaa gcacaccaat aaataccatg
     2101 aagctgaaga tatttccgat cacttctggt aagcctagga tttgacagtc aatcaagata
     2161 ggctgcgtga tatttgctaa ttccattttt ccatctggta cgacatttgc tacaagcatc
     2221 gcattgatga cattcaataa aactaatccg atcaaagcga taaatacgcc ttttgtataa
     2281 ttttttgttg cgtctttcaa acgaggcatg taaacagaac tcatttcgat cccaacaaaa
     2341 ataaatgtga taccagctaa gtatttcaat gaatcaatat gttccaagtc agggaaagct
     2401 tttgaccatg aaaagtcacc tagatagccg tttgagacta gtccgacttt gaaggctgcc
     2461 aaaacaccta aaacgaacat gatgactact gggatatata cccctagcca tacaccgatg
     2521 ttcccgccga cttttgccat atcgaatttc aagttcaaga tcgtgatgat ccagtagata
     2581 actaaaatac aacctaaaac gaaccagtga ttattgccaa gttctacatt accaaatgtg
     2641 tttcctaaca atggtcctaa agtagaagcg accattacca tacctgggaa catttgcacc
     2701 cataatagcc atgcaacgac aaatccccat ttgctgccca gtgctgtttt tacccataac
     2761 tgaggaccgc cttcttgagg aagcatcgtg gaaagctcac cggaaaccat tgatatcgga
     2821 ccggcaaaaa acaaaactgc aaaaattgaa taaaaaatca atgtccaacc gactgcggct
     2881 aacgtaggaa tactccgaac cgttgcacac agtgccattg tcagaccgat aaaggtccca
     2941 agacccattt tacttacttc agatttactc accgttgaca ctctccttga ttttttggat
     3001 aaaataacgg tgtctttgaa gtagtttcag caataaacga aacaattcag cgaatatttg
     3061 aggggattct ttgagtttat tgcttgaagg gtttcttcaa agacaacctc aaaacaaagg
     3121 gatcgaagag cttaggtctc cccttgctct tcagatccaa acatgcgtca gaaacagtta
     3181 gtattcaagc tgttttctaa catgattacc ttgcttattt tgcttcgctt gccaataatt
     3241 ggtctgcgta gattttttcc aatttttctt gaagagctgc tttgattttt tcagcatatt
     3301 cttcaaagtg ttcttctttg ttcatgtatg gtgtcattac gctggcacgc aatacagtta
     3361 cttttccggc acggttccat tcttcatctg agaatcctag ttggttcaca aattgtaatg
     3421 ggctgttgcc atagtctggg atagcaaagt cagtatgtga agttaagaac tcattaccgt
     3481 aaatgcttcc tttcacgtat gaagaataat catatacgtc atggttcaat ttattcattg
     3541 caactaagtc atcgttgcct ttttctttga atacatagtc taccatattg aaatctggat
     3601 aagttaatgg atgaacttcg atttctttgt ctcctacttt aaatgacaag tcattcaaga
     3661 agttgtagaa acgatgtgca ccttcgattg atgcacccat taatttacca tatcctgtga
     3721 cattcaaagg taagacatgg tgtgctgccc aaacgctggc tgctgttgcc cctgcttttg
     3781 aaccttccaa gatataagcc ccaagtaatg ctgggatatc tgcgcctttt tcaaatacat
     3841 atgtcgcaaa gtaagagata acgtctctca tgcggatatc tttgatgacg ataccaccgg
     3901 cagaatatgg aacatagccc attttatgtg ggtcaatcgt tacagattct gcttcttcga
     3961 ttgctttata tgcactgtgt acttcttcta agatataatc tttgttttct gtaaagacat
     4021 tgtatttgta atgaacatct tttaaatctt caaatggaat gaagttgttg tcttcgtcta
     4081 agaaaatcgc acggccgtat ccgccataag cagcatctac gtgtaagtag aagtaaatac
     4141 cgtctttttc taatacgcga cgcaatgcaa cgattttatc gataccgtcg attgctcctt
     4201 cttctgttga tcctacaaca ccgactacac ctaggattgg tgttttttca gctgctagac
     4261 cacgaacgat tttttcaagt tcgttgatgt ccatgcgata gttgtggtca actggcactg
     4321 gaatcacttg gtctaaaccg ataccgatga tgtcggcagc ttttagccat gaatagtgtt
     4381 tagtttgtgg aaccaaccat tttcctaatt tttctaaatg ttttccgcta cgtgctgagt
     4441 gagctttgat ttcgtcgatt ttttctggga cgctgtctaa cagattcatg atttcttctg
     4501 ttgacaagtt cattaattcc caatcgcttt ttccagcaac tagttcaggt gtcacttctt
     4561 tcattgcaag cggcaatgat ttgatgttgc gggcatacca aagtccttct aagtttgcta
     4621 aagaaccatc agcaacgatg tggccccagc catctttata gctcattaat tttgcaaatt
     4681 ccattcctac ttcttcttcc atttggcttg ttgcaggaga agattcatag gcaacgttat
     4741 taccattcca tagcatagca aaattgtacg ctagtaaaga tggcatcaat gtttcagagt
     4801 tcatatgacc ccagtaacgt ccggcattat gccaaggaac ggaatgggta cgcatccgcg
     4861 ctgaaatttc tgatagaaca tcctttgttt tattcacagt gtgttcaaaa ctagcactgc
     4921 ttttttcttc tggcgtaata ataggcatgt cttgaggcat gtagttttga cgccagccta
     4981 aatgttcatc caccaattca ttcaacaaag ctttatagat ttggccgttc tcagctttgt
     5041 ccccaatgaa tagggcattt aggtttagat ctttcgacaa tgattcactc atagtgaaac
     5101 cctcctaaaa aaatatttaa attttgtcga gccctacatt acacctgttc ttctattctg
     5161 taaactaagt tttggcctga tttttaatca tattttaata tgtatcaatt atccgaattg
     5221 ttcacacttt tgaaataatt aaaaaaaacg agggaattta caaatgtaaa ctccctcgca
     5281 aagattgtat taacgacaac cagcgttcta ttttaggatg ggtttcaaaa ttggaatgtt
     5341 gtaatagtat ttcccttttt caaggtaaaa acttactaaa ttctaatcaa ttactttggc
     5401 caaaaagtaa tttttcttac cttttcgaat aaccacaaac ttaccatcga attcatcgga
     5461 tggatttatg acaaaattta aatcggtaac gcggtcaccg ttaatactta tagctccgtt
     5521 tgaaacatct tcacgtgctt gacgtttaga tggttcaatt tttgtggaaa caagcagttc
     5581 cacgatattt tcaggtgtgc ttgaaatttc aacgttcggc attttaccga atccttgcgc
     5641 gatttcttcc gcatttagtt ctttaatatt tccagagaac aatgcttgcg ttattttttg
     5701 cgcttctttc aagtcttctt cactatgcac aaatcttgta acttcttctg ccaaacggcg
     5761 ttgtgcttca cgtttctcag gttctgtctc tacttttttc gctaagtctt cgatttcttc
     5821 ttgagacaag aaagtaaaga atttcaagta tttcactaca tcgcgatcat cttgattcaa
     5881 ccagaattgg tagaactcaa atggtgaagt tttcttcgga tcaagccaaa cggcaccgcc
     5941 tgcagttttt ccgaatttcg ttccgtcagc cttcagcatc aatgggatcg ttaaaccgaa
     6001 cgcttctgct tctggtcctt ctaattttcg gattaggtct aatccagctg tgatatttcc
     6061 ccattggtct gctccaccga tttgcaattg cacatcgtat gttttatgca gatgcaaaaa
     6121 gtcgatagat tgcaggattt ggtaagtaaa ctctgtaaat gagattccta cttccaaacg
     6181 gctggcaacg atatcttttg ctaacatcgt gttgatgttg aagtttttac cgtagtctct
     6241 caaaaattca agtaaagaga tttttgacaa ccagtcgtag ttgttcacaa atgtgatatt
     6301 tgcatcttta ccaaacaatt ttctcatttg attggaaagt gcgtccacgt tatgttgcac
     6361 ttgttccatc gtttgcaaca cacgttctgt tttgcggccg cttgggtcgc cgatcgttcc
     6421 tgttccgccg ccaatcagaa tatatggatg atggcctgcc aattggaatc ttttcatcat
     6481 cataaatgga atcaaatgtc cgatatgcat cgaatcacca gttggatcaa cgccgcaata
     6541 aagtgaaatg ctcttttctt caacgagctc gcgaagtctt tcttcgtttg tttgctgatt
     6601 aatggcgtcg cgccaagtta attcatcaat aatattcatt ttactgacct ccgtgtagtt
     6661 tttttatgaa agcaggaacc tgctagcatg tgtaattaat tatttcttca ggataggata
     6721 ggaaataaaa aaagtccctt ggcatataag ccaagggacg aaatctcgcg gtaccaccca
     6781 atttgtatgt ctagatatcc aacatacctc tcgaacattt aacgctgtcg cgtggcaatc
     6841 actgccaaag actcctgaaa tgtaattcgc tttaaagcat gtactggttt gcagcgacca
     6901 ccagctttct gaaacattgg ctttaacaac tacttcgttt cgatcttcgt cttgttatta
     6961 ttaatatgtg tctatcatag agcgcaactt tttctttgtc aacagaaaaa ggatattttt
     7021 tttaaattcc tcattttttc ttttttgaat ccgctctaat tccgaacatt ttattttcaa
     7081 ttattgttaa aagaaataac tttttctgta ttttttctgc aatttatcga gaagtcactt
     7141 tactttgcct cttattcttt cttcgttaca ataaggaatg ggacaagtaa aaaaatgaaa
     7201 aagcaaggga cgaggtatat ggaaaaaaag caaaaaatta ttttaggagc cacttctgct
     7261 ttaactatcg ttattttaat tctaagtttg ttttttgtca tccgatcatc tacaaaaaaa
     7321 gaaccaatca ttcctgaaaa aaaaataaca gaaaaagcaa aagaaccgac agatgataag
     7381 caacaactag ttactcaagc aaaaaatctg gctcaaagct acgattatga ccaagcgatt
     7441 gcgttattgg aaaaagatag taagaacgga tctactgaaa cacaacagct tcttcaaaca
     7501 ctaaaaaagg agaaagaaca actgatcgac tggtctgatc ctactcagat ctctcatgtc
     7561 tttttccaca gcttgatcgt ggacccgcaa aaagccttcc actctcaaca ggcacaagga
     7621 tacaaagatt atatggtgac tgttgaagaa ttcaaccgtt cgatcgaaca actttatcaa
     7681 aatgattttg ttttagttaa tttgaatgat ttgatccaaa aagacgaaca aggaaatctt
     7741 tcctttaacg ggcttgcgct acctgaagga aaaaaaccat tggttctttc acaagatgat
     7801 gtcagctatt atgaatatat ggataattcc ggttttccta gccgattggt cgtatcaaat
     7861 aacaatgaaa tcaaaaatgt ctataaagag aaagataaag aagaaatcgg tgactttgac
     7921 atggttcctc tgatcgatgc ctttgtcaag aaacatcctg atttttctta tcgaggcgca
     7981 aaaggtacct tggccctgac aggttacaac ggagttttag gttatcgaac atcaaaatct
     8041 caatatggag acaatgaaaa aacaaaaaaa gagattaaag aagctaaaaa agtggcggaa
     8101 cagttgaaga aagacggctg gagctttgct tcccatacat ggggtcactt gaatatgact
     8161 caagcttcac tagaagatgt aaaaaaagac aatgaattgt ggcagcaaga agttgcacct
     8221 atcctaggaa aaacaaatat tctgatttat ccatttggtg cagacatcag cgactggcag
     8281 ccttattcag cagaaaatca aaaatttgct tacttgaaga gtcaaggttt cgatattttc
     8341 tgtaatgtcg atgcttctac accagcttgg ggacaattag gtacggattt ctatcgaaat
     8401 gctcggatca atatcgatgg cattcgtttt caatctgatt tagacggaaa aaatcctatt
     8461 cttgatccat tcatcaatgt ccatgatgtc tatgacaaag aagcacgcga atctgcttga
     8521 ttttacgtca tttatgaacc aaaaaagccg aactgacatt ttatcgtcag tttcggcttt
     8581 tttatgcttt atttcttctt cggtgcgatt cccttgatca ggacttctgt ccattctgct
     8641 gtatatggaa gcagttttgc gtatatctcc ggcttagaaa ttccttctac cattgcttga
     8701 aaatagagga tcaagaactc atccgaatat ttcaaatcca cttttccttc tttccgccct
     8761 cgatggaaaa gatcaagcat gatactgaaa ctttcttgcg tatacgcatc catcgcgtca
     8821 gaaaattctt ttttttcttt ttttgtatag tatgtcatca aatcgatata aaattgtttg
     8881 ttcactcgat tgagataacg gattttgttt tcactcatgg caatcagtgt ttcttcaaat
     8941 ggaagatttt ccgcaatcaa cgctttcgct tcttgcccta aatcattcat aaatgcaaaa
     9001 aatacttcat aaatcagccc ttccttgctg ccaaaatatt taaaaatcgt cgctttactt
     9061 acctgagcgt tctcagctac cttatccatt gtcactccag cggttcctat gtctgtgttc
     9121 atcaagtcaa aagctgcttg taaaatctct ttctttttct cttgtgctct cttttcaaaa
     9181 ccgttcattt gtagagcctc ccgtaatttt aaacttttat aatatatata gttcaaattt
     9241 attctgaggt ttattataac tatctattga gaaagtatca agctagcaat tccttgttta
     9301 tgaattcatt gacagaaatt tataaaaaga atattatgta ctaaaataat aaaaaaaagt
     9361 tttataaaaa ggtataaaac ctttttatta aactatttaa tcttgtttag tatatatttt
     9421 tagaaaaagg agtggggttt ttatggcaga aatcgtcaaa gtcacacatt tgaaaaagag
     9481 ctttggtcgt ttcgaagctt taaaagatgt ctctttcacc gtacattcag gtgaagtcgt
     9541 tggatttatc ggaccaaatg gttctggaaa atccacgacg attcggacac ttttagggat
     9601 tataaaaaaa gacggcggaa cggcaacgat ttttgggcaa gatgtatggg aagatagttt
     9661 atctatccat gaacgtcttt cttatgttcc aggcgatatc tctttgtggg gaaatttgac
     9721 tggaggagcg atcatcgatc tcttcatgaa gcttcatgga aaagggagtg ctcaaaaaaa
     9781 agatgaactg attcaacgtt tctctcttga tcctaagaaa aaagcaaaaa actattcaaa
     9841 aggaaatcgc caaaaagtgg gcttgattgc tgctttggct gttgattcgg atctctattt
     9901 atttgatgag cctacttctg gtttagatcc tttgatggaa acgattttcc aagaagaagt
     9961 tgaaaaattg aaagaacagg gcaaatccat cctactttct tctcatattc tcagtgaagt
    10021 cgagcgacta gcggataaag tcgtgatcat acgcgaagga acactcgatg aagaaggaac
    10081 actcgatgaa ttgcgtcatt tgactcgttc gaccatcact atagaaacag aagcagagaa
    10141 tagtcactta gcacaattga acggtgtcca tgactttgtg caaaaaggtc acctcgctca
    10201 atttgctgct gataacgaag ctttcgatgt gattttagct gaagcgtcca aattaggtgt
    10261 gaaaaaattt gaatctattc caccgactct tgaggattta ttcatgagac attatgaagg
    10321 atgaggagga agcgaaatga ctgataaatt ttcacgctgg tccacccttt tttctcaaaa
    10381 ccttagccgt gactggaaaa agatcctgat atggctagtc ggtgtcactg ctttttctgg
    10441 tggttttgtc cctgcgtttg aagaattgac ttccggacaa ggattagctg gattatacga
    10501 aaccttgcaa aacccggcaa tgattgcaat ggtcggacca actccagctt cgaatgctgc
    10561 tgagtatact ttaggagcga tgtatgcaaa tgaaatgttg cttttttgca gtttgtttgc
    10621 tgctgtgatc tctgttttac acgtggtgag ccaaacgaga aaaaaagaag aacttggtct
    10681 gattgaactt attcgttcct ttcgagtcgg acgtcaagca aactctttag ctgtcatcgc
    10741 tgaaagtatt ctgatcaatt tgcttttagc aatatcgatc agctgtacga tgattttttt
    10801 ccaagcggat tcgatcactg caaaaggtgc ttggcttttt gggctatctg taggtagtat
    10861 ggggattttg gggagtgccg ttgcactgtt tgctgcacaa ctgatgccaa gttcagccag
    10921 ttcgaatggt ctgtctttag cgtttttagg tggactttac cttctacgag caataacaga
    10981 tgtagctgat tcctcccttt cgatgtggaa tcctttaggc tggtcttatt tgacctatcc
    11041 atttacaact aacaactgga ttcctttgct ttatttgttt ttgttcagtc taatcttgat
    11101 aatcagttca tttgttttag aggaacatcg agatttgaac accggctata tccccgaacg
    11161 tgaaggaaaa ccgtatgcta aacgttctct tttatccttg cgtggtttat tattctatct
    11221 aaataaaggg atgacaatca gttggcttat cacttttggt ttgatggggg cggcatacgg
    11281 ctctatctac gggaatatgc aacagttttt agaaagtaac gacttaatga aacagatgtt
    11341 tatccaatct ggcgtctcaa ttgaaaaaaa ctttacaagc acgatcatgg tagtcttgat
    11401 ttgtctagct gctattttac caattgctag catcaatcgt ttatttacag aagaaaccca
    11461 cggacgtatg aatcagctgt tcgcaacaaa aatttctagg aaccaacttt attggaccaa
    11521 tatgttacta gctattctat ttgctatatt ggcaatcctg attagttctg gtgcactagg
    11581 gctttctgct ttggcttcga tgaaggatgc gaaagacatg gatctattga tgtttcttaa
    11641 agccgggttc aatttcctgc ctgccgtttt agtgtttatc ggaattagtg gctttctatt
    11701 cggatggctt cctcgaatcg gtaaacttgc ttatatctac ttagcttact cctttgcact
    11761 caattacttt ggcggaattt tggatttacc agaatggttt tccaaaacag ctgtccctag
    11821 ctgggttcca cggatgccaa acgattcttt tgatctttcc gtgttcctta cgttatcatg
    11881 tatcagcctt gcactgttcg tccttggttc tattggttat aggcgaagag acttactaga
    11941 aggataataa aaaagcatgt acaatttcat gctagcctcc aaatctgggt caaaaatcca
    12001 acatttggag gtcatttttt tatttacaag tcttctaact gctctttatc aatgatccag
    12061 acttctcgat tctttctttt tacataacca ttctcctcta ataatttaaa ttttctagat
    12121 aaagtttcgg gggtcgtacc gataaatgca gcaagttctt tcattttcat tggcaggaaa
    12181 aagtgatcct tccctgtcgc tttagctaag tcaagcagat agacagcgac tcgttcttct
    12241 actttttcca tcatcaaaaa agttgtctgc tgctgtactt ttgttaattt ctcaacgttg
    12301 attgctaaaa gtttcagact aagggcggga taatgcagta agagttccga gaaatctttt
    12361 tgtctcaata tgcaaatttc cgttttttct agtgcttcgc caaataaaga ctcatttgga
    12421 acgcctagca actggctctc tccctcatat ccgcccggct ctattactcg taaaagctgt
    12481 tcctttccgc tagcagataa ttgatacact ttcatactgc cggaggccac gatcactaat
    12541 tgaggttctc cattcggcgc aagaatctgt tctccttttt ccacatagtg atgtttgaca
    12601 agtgcgtgta ctttttcttg atcttgctgt actaagtgat tgaacaaagg aacaaatgaa
    12661 acacataaat gatcatgact cataatctct cctccttcta aacagctatt tctaataaag
    12721 aaacataact tcccttattc caactgataa ttttctttta tcatagcata aacgtcaagc
    12781 gaaccagata ccagcaaata gagtggattt ctttttgtcc cttgctttat tttccttctt
    12841 ttgagacctt aagtaattga ttcctatcaa ttttttaaat atttactttt tgaataatca
    12901 aaaacaaacc ttcactcgca agccgagagt aaggtttgtt tttcgcattg cttatttcgt
    12961 gttgtctatt gaaatttttc cgcacctagc ttttgatata gtggtgctag ttcttgtaag
    13021 gtttcagcgg ttatttgctc aacgtgttcg atttcttgcg gatagaagaa tagtttaccc
    13081 atttgaaagg ttcggtctct catcgctttt ttcaactctt cttgtttctt tgtatcggac
    13141 aaatagtcag acaaagctaa gtggtcgtca agttcatgtt ctggttgcgg ccatatgtaa
    13201 taatccttga tatcgactgt tttgctccac tctttcagta tcgcaagcaa ctggttatac
    13261 gctgcaatgc taccatatcg ttcttcgctt ttataatgtt ggtacatcaa atagatttgg
    13321 tattgttttt gattcaacaa aactgctaga caggcattct cgtcttgacg agattcgctt
    13381 cggtacgcgc tccaaaaatg actgcgtaaa ttccagccat tcgtccagct ttcgatttta
    13441 ggtttgctca tgccgtacat atcaggcaaa agtgcagctg tctggagctg aatctctttc
    13501 catttttgcc agtgttcttt atattctttc ttgatttgtt caatcttatc tgttggatgg
    13561 ctctctttga gctgtttgaa tgcgtagatt ggtcgatcaa acaactgata atctgatttt
    13621 ccaagttcca ttgtttcgcc tctttccgtt atcttttgta ttctacctga attttgcgat
    13681 atggacaaat agaggttgtg aaaaagctgt tctgcatcaa gtcataagaa cagccaaaca
    13741 aaaaactgcg atgagcatca ccgcagtttt attgaactta aggttgattg atcacatcta
    13801 aaagcgttga aaataaaatg gcactagaag cagctcctgg gtcgatatgt ccgattgcac
    13861 gctctcctag ataggaagct cgtccttttt tagctaccaa gactttggtg tgttcttttg
    13921 cctggtcaat gatttctttt gttaaattcc ctgccttcaa tgcctctgtg actggcagcc
    13981 aaacatctaa catcgtttta tcgtcagctt ctgcctttcc tcggctttga atacccgctg
    14041 ttccctgctc gatcacttcg cctaattttt cttgagaatc aatagaatcg acctcttttg
    14101 tcgctttcat catgttcatg aatgcagatc cgtataacgg accagaagcc ccaccgactt
    14161 tcgatatcat cgccatagag aatactttga atgtatcgct gattgtttca ggatgatcgg
    14221 cttgaaaagc ttcttcataa gctgaaaccc ctcttcccat gttatttcca tggtctccat
    14281 cgccaatcgg ggtatctaga tcactgagat attgtttgtt ctgattgatt gcctctgcaa
    14341 attgcgtgag ccattcttgg atttcttgaa cggttagttt catattatca cccttttatc
    14401 ttgagatttt ttaccatgaa atcgtctgta cattgctttc taaagaatct ttccattgtg
    14461 tctccgtcaa gtcgatcagt gttagagaaa gaccttgcat atctagtgag gtcataaaat
    14521 taccgacttt tcggaaaact acattgacgc ctctttcttc caacagagct aaaacatcat
    14581 tcatgaacac gaactgttcc atcaaaggcg tcccgcccat tccgttgact agtacacctg
    14641 cttcttttgg taaattcgag taatcatcta aaatcttagt aacaagttcc tttgccagta
    14701 ctttagaagg ctgcatcttt tctcgacgat agcctggctc accgtggata ccaacaccat
    14761 actcgatctc atcttctcct agttcgaatc ctggtttccc tacttctggt acagttgctg
    14821 ctcgaagcgc tagaccgatc gtcttagttg cctgtacgac cttttctccc agttctttca
    14881 actcagctag ggaagcaccg tttcttgctg catcgcccac gattttgtgt acaaggacag
    14941 tacctgcaac tccgcgtttt ccagcagtat acgtactatt ttcaacggcg atatcatcat
    15001 ctacgatcac actttctact tcaatatcgt ccatagcagc taattccttt gccatatcga
    15061 agttcaatgc atcccctgta taattcttaa cgatcagcaa aactcctttt ccactatcgg
    15121 cttccttaat agccgtctga atctgatctg gagttggaga agtaaacaca tcaccaagca
    15181 ccgctgcact taacatccct tcccctacaa acccggcatg ggatggttca tgtccgcttc
    15241 ctccaccaga aaccaaacca acttgttccg ctttctgatt cttggctaca acacgggaag
    15301 attctacacg atgaacaagt tctggatagc tttttactaa tccttccagc atttcttcga
    15361 caatattgct tggatcattc atgatttttt tcaatttatt cccatccttt ctttccggca
    15421 ttttgtgctt ctgttaagat ttgctgagta tcatctgtta taccggcagt gatggctgct
    15481 gcaaaagctc cttccactaa aggcgcatcc accagatgat aacgttgctg ctgctcttct
    15541 tccaacatgt cttttgccag ttccgagttc aataccgcgc taccgatatc cgcaaaaaca
    15601 aaataactag ctgcatcgct tgattcattg actgcttcta cgattttcat cgggtctgat
    15661 ccgatctcgc catttattcc gccgagagaa aaaatttgca cctcttcgga tttttgcatc
    15721 tgctcaatca tttctttaat accatctgtt actttttgac tatgagaaat aagcaaaata
    15781 ctttttttca caagaatctt cctttctttt acatagttgc taacgattgg tttcgctttc
    15841 attaatgaac aattcaagta tataataaat ttctaatttt gggaaaatat gtgtagtgct
    15901 tcttgaagtt tgttatactt taattaaaag agaggagtga tcgaatgatt acaacaacaa
    15961 cgaacagtgt agagggaaaa gccgtagtag cttataaagg gatcgttttt ggtgaggtca
    16021 ttactgggat ccatgcattt aaagacttgg aggcggggtt gcgaaatatc tttggcggcc
    16081 gctcaaaaag ctatgaaaac gaactaatgg gtgcaagaga agaagcactt gccgaaatgg
    16141 ccgaacgtgc aaaagcatta ggagctgatg cgatcattgg cgtaaaaatg gactatgaag
    16201 tattaggcag cgacaatggc atgctgatgg tcacatgtag tggaacagct gtctccacga
    16261 gataagtagg ggacatctgt tttaataact aaagagagat gggacatgta gtggcactca
    16321 agaattagat ttttcagtct aacttcgagg tactctacct tcccatcctc tcttttttat
    16381 ttctttttgc ggatgattcc gtaagctgct gcgactaaca cacccgcccc tgctaaggaa
    16441 taactgacag aagctgtttt ttcatttgtt tttggcaaag tctgacttgt tgatttggat
    16501 ggctctgtcg tttgctgaac tgtttctgtc gtgcttccag aggtcactga tttttctcct
    16561 gccgaatgca aaatatcttt tattcgttgg aaactagctg ctaagtcagc tttttttgct
    16621 tcaaaatcat tagcaactgt ttgtgcttcg ctgatctttc cgtggattgc ttgactggcc
    16681 gctgtgatat ccgcttgagt ttgacttagc tttccagaag ctgtttgagc aacctgattt
    16741 aattgctgat tgaattcttt tgcttgttgg atcttcccgt caagatctgt ttggacagct
    16801 tccgcttttg ctttcgcttc ggcaagcttt ttctcaagtt gtgcacggtc atttcccact
    16861 ttatttttaa gatcagctaa atcttgatcg attgctgctt ggtcttcttt caatttgttt
    16921 tttgcagctt ctaatgcttc ttcgagttct tttcttttat tttccaagtc ttgcaaatga
    16981 tcgatttggt cagaagaggt agaactattt attgatgtat cttctgaaga tgaaatcgtt
    17041 tctcctgaat tatctgtcgg tacattggaa gtcatttctg tttccgaggc accgttttca
    17101 gcaccagaag agcttatggt tgttgaaggt tctgtttgag caggttcaga agtttccgca
    17161 aaagctgtct gtcccaataa gaaaatactg gaaacagcaa ttggccagat taacggcttc
    17221 attctttttt tcatcacttt tcccatcctt actacttttt aactaatttc ttattataag
    17281 caaaaatgtt tggaagaacc ataaacacaa caggctgttt gtctaaaaaa cggtgcatct
    17341 tttatttttt tgtacgttcc gggcttttcc atgtcccgct tccataacct aaaatcgttt
    17401 tgactgctgg aatgaaagtc gtcacgatcg ttatcgcgtt cactatccag aacaaaagca
    17461 tatatagtgg tgcgaaaaga aggtatttca attttcttcg tcgatcatct acaatcagcg
    17521 aagctagcaa ctggaaaaat cctgcgacca tttcaaagca gatgaacaca aaactgaatg
    17581 taaaagtaat ataaatctgt tcgtagtttc cttgatatac ataatgcccg attaatccag
    17641 caaaaagaac tacggaaata caaaagaata ttgaccagac aatactcaat gtttgatcaa
    17701 tgaagatgat cgttcttcca atatgtttaa aaggatggag catcactttt ttgaaattcg
    17761 tcagccagac ttctgttcca ccttttgccc aacgttttct gtgtcgatat agcatcttca
    17821 acgtcacagg tacttccatg aaaaagataa tctcaggagc aaaaacagat aaccagtcat
    17881 ttaactgatg atcccaagca atactgatgt cttctgtcgc tcgatcttgt ctgaatccgc
    17941 ccacatcgat caaggcttct tttcgataca tcgtatttgc tccactgtac gcataaagac
    18001 caccgaaaac agctgactgt gttcgtttaa tgatgcccac gatacttgag aattcaaccg
    18061 tttgagattt tgcaatcaat ttggtacgat tttggacatc catatttgca gtgaccgcag
    18121 caatatgtct tgcaccaggg cgaatgaaat aattcacata cctaatcagt gcatctggct
    18181 ccggtaccgt gtctgcatca ttgcttaaaa tcaatttccc ttttgcaaag gcaagtccga
    18241 tattaaacgc gtgggctttt cctttatttt tttcgatccg aaccacacga agattagcgt
    18301 attttttcat cagtctagct aatatttctg gtgtttggtc cgttgaacca tcgtctgtca
    18361 ctaatacctc atagttgtga taattcaatt tcgtcatcaa atactcgatt gtatattcga
    18421 tcacaatttc ttcattatgg gctggcacca tgatcgtgat gaacggttcg acactcaagg
    18481 gaacatctac ccattctttt tgcttatgtt tgtataaaaa cacataacac cagacaccaa
    18541 taaaccatac aaatccgcct aaaatcggat aagaaacgag aaaaaaatac ataatagaca
    18601 aacaagtatc taagaataaa ttacctgtat gggtgattga gatcatttca gttccacccc
    18661 attttcttta tacaaatctg caatgaatgt cgtatctaga tttttctctt cggagacaga
    18721 ataaaaacgg accgtttctc tctcttcttt tgttccaaaa cgttccgtat acacttcttc
    18781 aagtaattct tgtctttttt tcaatttttc atcttcatat tcaaaagatt tatttacttt
    18841 tttgtataga tttcgattgt tccacctagt caatacaaat gaggtaatca ggaaactgaa
    18901 aaaaatcaat acaaaaaagc cgattaagaa atacagcatt ttcttttctg ataaaaaagc
    18961 gtacaaggaa aaatccttgg ctctttctgg tctcgttaat gggaaaaaga cccatataaa
    19021 aggaagaaca acgatcatcc atcctaaaag agccactaac gtttgccaaa tctttaacca
    19081 gtaattacct tttgaaaaat acttatcttt caccaaaatt gatgcaactt cttctcttgt
    19141 tacttttttc atatcctcac ctcacgtttt tttgttacga cgtaggagtt taccatacat
    19201 ttcatttctt ttcacataaa gtgttcctaa atttttccta agaaaaagaa caaaacattt
    19261 ctcatcagca aatgaaaaat attttgttct ttttatcagt tgttcttttt attttttatc
    19321 tcttaattat gttgctcttt ccaatacgac aatgcttttc attcctctag atatcgccgt
    19381 gtacatttgt ctgcgtcttc tttgctcatc tccatcgttt tcaaatggat ataagattac
    19441 atgatcaaat tccagtcctt ttgctagact caaaggaatg atttggcagt tttctaatcc
    19501 atatgaattt tttttaatcg attcttgtag cgtttgggtt tcctcttctg tttgggtgat
    19561 aactgccgct ttttctcctt ttagagaaac caaaatctca gctagtattt tcaacaattc
    19621 ttcagaggat cgaaaagagc ggtattcggg ttcttttcct tgcggtcgga tcgatacgat
    19681 gtcgatcttc tcttgatcca cagcataggt tttgaaaagc tctgtgattg ctccgctaga
    19741 acggtaacta tttctaagat cataccgatg gatcggcaga tggtagtcgt cgaaacaacg
    19801 cataatatca gcaaaagtag tggaagaatt aaagattgcc tgattctcgt cacctactaa
    19861 agtaaagtga gtttttggaa ataattcgat caatagacct aattgtgcca gggtataatc
    19921 ttgtacttca tcaatcaata gatagcgaaa tgctggtaca tttacctttt caacaagtaa
    19981 atgacgaata gctaatataa tgactgcttc atcaacagta cgtgtgggtt gccaagcata
    20041 agctgagcca tatcgttttt cataaattct ttcaaacaac tgatgctcat ccacccatgc
    20101 catttcttcg atttgtctgg ttatttttcg atattttttt tgtaaaagtt tccttgcata
    20161 agctacaata ctttgttccg aatcatcaga aatcaattta ccgaataatt ccaactgcct
    20221 atcttctgtc aacgagagta cctgattctg cacagcagga ctttttgctt gtttcaaaag
    20281 atgattcttc cattcgctca tcagtgcctt tttcgttgct tgaagcctat cgatcaattt
    20341 aggatgagca ggaactttct catatatttt ttcgatcgtt tttttagaaa ggactgtttt
    20401 tccattttta gtaagtccac ggataaaatt cggatgatct aaaaacaaag gatctgattg
    20461 ttttagatta tcgataaact ttttgctacg caaacgttct gtctgctccg atacattttc
    20521 tcccgtgata cgtttgaaat aagcctcttc accttctatt tcctctgctg aaagctgtga
    20581 aaccagctgt atgatggtta gattcagtgg atttctttca cctaatgatg gtaggacatt
    20641 cgaaatatag tcgataaacc gatgattagg tgatagaatc agcaagtcat cggatgtcat
    20701 cgtatctcga tataaataga gcagatacgc aatacgctgc atgatagcag aggttttacc
    20761 acttcctgct acgccattta ccaagatatt ttgacttttc acatcccgaa tgattgtgtt
    20821 ttgttccgat tggatcgtag cagtgatagc cttcatttca ttggtctcat cttggcctaa
    20881 gacttctaat aatacgtcat cttgaatagc tacagacgta tcaaaaaact tgaccaaacg
    20941 atctctttcc aaaagaaatt gtcgtctttc tttaataacc gtatcgattt tattttgacg
    21001 aaccgtataa gaagatggac caatcatgtt attgtaaaaa agttcggcaa ttggtgaacg
    21061 ccaatcataa acaagatttt catctgcatc atctgtgaaa tttgctgtac cgatataaaa
    21121 ggattctttt ggctcattat ccagaaagtc tacaacgact tttccaaagt agggactttt
    21181 caataaacgt tcgatctttg ctagctgatt ttctgcgttt ttctgtttga tattcatctg
    21241 atcgatttct ctatttttca tttcaatcat cgaaaaagta tccaggttat ctaagtaact
    21301 gtcaaaattc aatcgaagct cgccagccat ttctttcaac gactggtttc cttctatttt
    21361 tgccgtatcc atcgattccc gcagttcgtt ttttttatca attagctttt gatacacgtt
    21421 cttcagatat gcttcttcta actgtttttg tgtgttgacc atacacattc tcctcttttt
    21481 ttaaagtggt ggaaccgatt agtttacctc ttttaagagt cgaatgcaag ctttttaacg
    21541 agtatcattt gttaggatat atcagttctt caggtcggac agtttgatat aaagaaaatg
    21601 caccatcaag gttttttacg tggtagccag actgcttcaa gattctttcc gctagatagc
    21661 tacgtaatcc gctatgacag ctgacgatat attcttgttg gctgtctaat tcatttaatc
    21721 ggtcccgaag ttcattcaaa gggatatgtt tggcttcctt gaatttgccc ttttccaatt
    21781 cttctgctgt tcgcacatct aataaaattt ttccttttgc tagttctgtc ggcaattcat
    21841 gccattgtac ggtttcacta agcccttctg caatgttcat tgctgcatat cctagcatgt
    21901 ttactggatc tttggctgag ccaaatggcg gtgcatacgt caattctaat tccggtaaat
    21961 cgaagatcgt caaatgccct ttgattgctg ttgctaaaat atcgatccgt ttatccacac
    22021 cttttgcccc aacaccttgt gcaccataaa tctcgccagt ctctggatgg aagatcaact
    22081 tcagtaaaat atctgttgct tctggataat agcttgcatg atcttttcct gatacatgaa
    22141 tgacttcaaa agacagttcg ttttctttgg ctgttctttc acttaaaccg gtggatgcgg
    22201 cagtcattcc aaacgttcgt ataattgctg ttccaatacc gccttgattt cttcgagaaa
    22261 ttccggaaat cgtgtccgca acttgtctac cttgacgatt ggctggtgaa gcaagagaaa
    22321 taagtgcatc ttttccagtg atttgctgtt ttacaacgat agcatccccc accgcataaa
    22381 tatcaggaat attcgtttga taacgttcgt ctactaggat acccccgcgt atgcctaatt
    22441 cgattccagc ggtcactgct agctggttat caggacgaac accaatggat aaaataacta
    22501 aatcagcagg gatggtacgc ccatcatcca gtcgcaatcg tttcccgtgg tcttcaaatc
    22561 cagcgacagc atgagaagta atcacttcta ctccttgatg agacaattct ttttctataa
    22621 aagctgccat ttcttcatcc agtgttggaa gaatatgtgg tgcttgttcg atcaccatga
    22681 cttctaaacc tcttcttttt aggttttctg ccatttccaa tccgatgaat cctgcaccaa
    22741 cgatcacggc acgctttggc tgtttttcta atgcatgcat cactttatcg atatctggta
    22801 catttctaat agaaaaaaca ttgtcggctt ctgttatccc tggaatcgat ggaacaactg
    22861 gttttgcacc aggagataaa accaatgcat cgtactgttc aatgtgtttt tgaccctcat
    22921 gtacgacttc taccgttttg ttttcgggaa agatggctgt cacttcatga tttggacgca
    22981 catctaaaca aaaacgagcg gataatgatt ctggcgtttg gacaagaaga ttttcccgtt
    23041 cactgatttc tcccgaaaga taataaggca agccgcaatt tgcaaaagaa acatacggtc
    23101 ctttttcaaa aacaacgatt tctgcatctt ccattaatcg gcgaagccgt gtagcagcag
    23161 acattccacc tgctacacct ccgacaataa cgattttcat cttatttccc tcctactgtt
    23221 cgatcaaacc attgattcat gccaccacgt acattcacta catcataacc agatttcttt
    23281 aattctttag ctgcttgttt actcctcatc cctgattgac aaatcacata gacggtcttt
    23341 tctttgtctc catcatatct gtttatactt tgtaatggaa catttttagc tccttttata
    23401 tgcccgcccc ggtattctgc aggtgttcta acatcaagta acgctggatt ttccaaaagc
    23461 ttttccttta attctgggac tgttatcgat tttattgatt gaaatagcaa attcattttc
    23521 tgcacctcat ttacattttt ataaatacct catggggtat attaaacagg agctttatta
    23581 atatgtcaac cagaatgact cttcattttt ttcactactt tttatatttg cataaaatac
    23641 cgataagggg tattatctaa ttataaaaat agaggagaat gtgttatgga gattgataat
    23701 aaaaatatcc tgaaccgttt gaaacgtaca gagggacaaa ttcgtggtat ccaaaaaatg
    23761 atcgaagacg aaaaagaatg tatggatgtc atcactcaat taagcgctgt tcgttctagt
    23821 attgatcgcg taatgggcat gatcgttgca gataatctga aaaattgctt tgagaaacct
    23881 gatagtaatc cagaagaaca agcggctaaa ctagaacaag cgatcaagat gattattaaa
    23941 aagtaaagga atgatttcaa tgaaagtcgt tttccatatt gatgaattag aaaaatggtc
    24001 agaaacaggg aaaaacgtaa aaaacctgat aaaagcttct ccagaaacga cgattgtcgt
    24061 atctgtaaat ggcatcgcga ttaccggtta tctagactct gccaatgcag aattcttaga
    24121 tttgcaagga gtcgtttttc acgcttgcgc caatgctatg cgtgctaatc atatttcaga
    24181 aagttcttta cctgaacaag taatcgttgt accagcaggt gtccttgatt tagttgaact
    24241 gcaatcccaa ggttatgctt atatcaagcc ttagtcagta gaggttgcga cagaagtgat
    24301 cagcttcaag aaatttcggc ttatttttcg aagaagctac ttctggaaaa ccgtgaatca
    24361 aaggttgtga aaacgctgtc ctgcatcaag taataagaac agccaaacaa aaatagcttc
    24421 tcatattttt cgtttggttg ggcttattac cgcagatgca agcttttgaa caccgtttaa
    24481 ggaagaggtt gtgaaatcag cggtttgacc taagtattag agaaaaaaac gggaaaatag
    24541 ctcctcatat tttgccgttt tttgagctaa taccgaaggt cagctgattg aacaccgttt
    24601 aaggaagaga ttgtgaaaaa gtcgcttagt ttcgagtatt aagaaaaatt ttgtgaaaat
    24661 ggatttctac atttttgcat aaatttggct taataccgag aaactggctt ttgaataccg
    24721 tttaaggaag aggttgtaaa atcagcggtt tgacctgagt attagagaaa caaacggaaa
    24781 aatagctgct catattttgc cgttttttga gctaataccg aaggtcagct gattgaacac
    24841 cgtttaccaa gaggttgtga gcctaccatt taactccgaa tcacaagaaa taatttcaaa
    24901 aacaacttct cctgtttttg gttcggttgg cttattatcg agaagtagct actgggagtc
    24961 tatttatcaa aaaaaggaat gcgacatagg tcacacccct tttttatttt ctttgaaaca
    25021 tctttcggta ctcgacagca tacaagacaa aagattgtaa aagaccgtaa gtgatgacgg
    25081 caaaaatgac tctcacccac agcgttggta attctgggaa aagcagcaaa aggatcatgc
    25141 caacatagat gacagcagtc gctactttgc cataccactt cgctccactt aatttgatat
    25201 ttttagatag cccgattaat ccagctacaa acataaagcc ttcttttatc aagaatagac
    25261 caaacaacag ccaaagccac ggtcgatacc atgccataga caaaatcaga aaaagttgtg
    25321 tcaatttatc tgcaaacgga tcaagcacct tccctaagtc tgttacctga ttatatctcc
    25381 gagcaatgac cccgtctaga taatcagaca aacctgagac aacaatgacc gctcctgcta
    25441 tataaaatga ctgtctcagc acaaaataaa gataaatcgg cagcaaaaaa atacggaata
    25501 tcgataaaag attcggaatc gtttgtattt ctttacgcca gtctgtgctt aatttcattt
    25561 tatcgcccta cctcctcagc actcttttct ttttatttta gcttacacgg ggaataaaac
    25621 caatgatttc actgttttta tttttcttta cagacactga tattatgagc taatttgaga
    25681 taatcacttg ctgttttcat cgtttactct tctttcttcc tgtcgtttgc gcatttcttt
    25741 tactagtact ttatgagctt tttttgatcc tcgctctagg tcacgctgcc gtttacgtgt
    25801 tttataatcg ataaacagac tgtctaaatc gtaacctgtc gggtacagat cttctgctga
    25861 acgcaccaga cgaacacgct gacgaggcac gtggattatt tttttgtcta aatatacatc
    25921 aatcgtatct tcacctatat cttcataaac taacgcttct ttttggtatt cattgacaaa
    25981 tatccgatca ccttttgcaa agagctgacg ttctttttta ttctccttcc tatttgtaac
    26041 tgctgtctta ggaagaaatt cttttttctg tgtccgatag gctttttccg ccaagtaacg
    26101 ctctgcttga tggatcaatt tcatcgacat atccatttta tgagcaatcc acaaagcttg
    26161 gctttctcct gtttcttcca acccgcaact gatatttagg acgaagtgct tcccgatcga
    26221 aagccatagc agccggcaca aaatcttcat gatctccggc gaatttcttg atttctccat
    26281 aatgggtcgt ggcaatgatc aatgcccctt gctcatacat agattccatg attgcgatcg
    26341 ctagtgcagc cccttcattt ggctctgttc cactgccaat ctcatctaaa agtaccaacg
    26401 tattcctttt tatctttttc aaaatagcag cgatgttttg catatgacca gaaaatgtac
    26461 tcaacgcatt ttctaaattt tggtggtcac cgacatcgac gaaaatctca tcaaagacag
    26521 caagctctgt cccttctttt gccggaactt gcataccgaa catcgccatc aaagtcaata
    26581 aaccgactgt cttcaacacg actgttttgc ctccggcatt tgctccggtg ataatcaagc
    26641 ctcgatagtc tttaccgatt tcaacatcca atggtaccgc atgctcaggt aaaaatggat
    26701 gtctgccttg cttgataatg atatgttccg atttattgat tctaggtgtg cttccattga
    26761 tttctcggct aaattttgcc cgagcaaaga tgatatctaa caccgtgatc gtttcgatca
    26821 ataaatcgat cacttcttcg ttttctgcga tagcaccagt caatgccgct aggacttgat
    26881 attcttcact gatttcttca gcttttaaca actgatagtg ttcattcaat ttagaaatca
    26941 cagttggttc aataaaaacc gtcgttcttt tatttgattc atcgataatc gttccatcta
    27001 ttttattttt atagctggct ttgataggaa tcgtatagta ttcgcctttt tgaacaatca
    27061 ttgcttcttg gatcatttct ttgtttttcg gatgacgcaa gaatttcagt aattttgact
    27121 ggatttcttt ttcgattagt tgtaattgtt tgcgtacttt acgcaaatta cgtgacgcgt
    27181 cgtcactgac tttattattt tttattttct ggtcaatcaa ctcttctaca tttatcaaat
    27241 caggaagatg tttgctataa gcaaacaaca agggtgtctg gtattgattc ttatcaaaaa
    27301 actttgtgat catccgactg cttctaagaa aatccgcata ttcgatcaaa tcggttggct
    27361 gcagaatcaa cccttttttg acttgttctg tcagtgcatc gatcctcggc aaccccataa
    27421 atggaacatg ctgactgctt tctaagatca agcgtgcttc tcttgtttct tcttgacgat
    27481 ccaagacagt ttgtaggttt gtcgagaccg tcatatcgct gattcgtttt ttgctatagt
    27541 ttccaattgc tcttgcttgg atttcctttt tgatccgatc aaattgtaat tgactaatca
    27601 tttcgttgtc catattctct ccttttcttg aagagatacc tgtcccaaaa taaataaggg
    27661 cctagagata aataaaagga tagaacacag cttaaaaaag tcatgtccta tccttattag
    27721 aatacaaaga tattgttttc ctgacaggta ccgtttcata agcacaccaa ataaactttg
    27781 tttattggct attcgactta ctgattagat accagctaca ctcacaaaac ataccccatt
    27841 cttctaggtc ttatttattt tccacttaac tatagcaaga cacattttgg ttgtcaaact
    27901 ttagtatgtt ttatagattt acaccattaa tgaatagaca cctaccgcga taattgctcc
    27961 gatcattgga cctgcgattg gtacccatga atatgcccaa tctgaagttc ctttattagc
    28021 tattggcaat accgcatgtg ctaatcgagg accaaagtcc cgagcagggt tgattgcata
    28081 tcctgttggc cctcctaatg aaagaccgat ggctaagatc aatccaccta cagcgaatac
    28141 gttcgttcca ggtgcaaatt cgttttgtcc aaaagctagc aacccaagaa ctaatacaaa
    28201 tgtaccaatt aattctgtga tcacatttgc tggataattc cgtatagcag gacctgttgc
    28261 aaacgttcct aaaatcgcgc tttcatcttt cgttgcattc caatgtggca aataagaaag
    28321 ccaaacaaga atggcccctg caaaagctcc taaaacttgc gcaatgatga aaggaacgac
    28381 aagattccaa ctaattgccc ctgtcatcgc catagctaga gagactgcag gattcaaatg
    28441 agctgggccc atgaatccag aaatatagac agccattgtt acagcgagtc cccaaccaaa
    28501 tgcgatgaca atccagccag aagcctgtgc tttacttttg tttaaattca cagccgcaca
    28561 cacaccatct ccaagcaaaa ctaggatcag tgtcccaata aactccccca tgatttgagt
    28621 catatcactg ttcatttatt tctccccctt tagttcccgc agatctgatt ctgcaatcaa
    28681 tgcttctaaa cgtttagtct gttgcgcctt ctcctcttct gtccattcaa aatatccagc
    28741 aattgcatcg atcacaggtt gcttgatctc atcaagctga tctcgttcaa acaacagatg
    28801 atttgtacga cgaatgagat aatcgcctgg ggccaaaacc atttcttctt ctaaaccata
    28861 acgtaaccga gctgactcag cgagactcaa accaggataa ggtgccattt cttttgctaa
    28921 ttcaaagata cgtcgggcat tagtcccgta aaaatcagcg atataagtag catcttcttc
    28981 ctctaaaccg gctgctactc cttcttttgc taattctgtc actgtttctt ctaatttagt
    29041 tggatcgaag tttccacctg aaatctgata ttttttagag tcgatttctt tcgtctctat
    29101 tccgtattct tcttttaata attggcgtat caatcgcaaa gctccttctg ccatcttacg
    29161 gtaatcggtg attttcccac cagataaagt cagcaaacca tctggttctc tttctaaaga
    29221 actacctctg gaaatcgtag aaggtgcttt ttcatcacgg ctgttttcca aatggttcaa
    29281 cacgtcttct acttcagcac gagaaacttt gttttcctta tattcactta ctgtatcgac
    29341 aactttattg aagctcttgt ctgaaatcga tccattatct ccaccattgt aatcagaacc
    29401 agaattacca atcaacagtg gacgaagtcc tgcccagctt gcttcgatat cattcaatgt
    29461 gatatttgct tctggatagc gatgattgat cacatccaat agataatcta catcttcttg
    29521 tgtgactttc ggatccgtaa aatctccttg gtaatccgta tctgtcgtac caaagtaagt
    29581 cttgttttct cttggaatag caaaaaccat ccgcccatct tgctttcctg tatcgaagta
    29641 tgtgggttgc ggtacaggca gttttttcgc atctacaact aaatggatcc ctttagttgg
    29701 acgcatttta ggagagactg gacgcgtaaa attcaagttt cgtactttat ctacccaagg
    29761 accactcgta ttgatcacta attttgcttt gatttcgatc acttcatctg tcagcaggtc
    29821 acgagctttg acgccaacga tttgatcgcc atcatataaa aagccaaccg ctttcatttt
    29881 acttactaga taagccccat cttcggcagc ctttttgata ttatcaatca ctaaacgggc
    29941 atcattgttg cggaaatcta gatagacacc cgcgcctttt agcccttctt ttttcaaaaa
    30001 cggttctctt tccaatactt cttctggtgt cagcgtataa ttctcatatt tggttcctgt
    30061 cacatttgcc agtttgtcat aaaggtccat tgctactttg accgagaaca tattgaaggt
    30121 tgttgctcct tcgccctcat agattggcaa aagcattgga tctggttttg ggatgtgtgg
    30181 ggcaatactt tgtacgactg cacgttcacc aactgtgtca gctactactt ccacatcaaa
    30241 tgtcttcaga taacgaatac cgccatgaac aagtttggtc gagcgagagg aagtcccttc
    30301 tgcaaaatcc tgcatttcga tcaatcctgt tttgattcct gatgctgctg actggactgc
    30361 cacaccggca ccagtgattc cgccaccgat gatcaacaga tccaattctt cttgctgcat
    30421 tttttgaatg ctatcttgtc ttgtcttgtt tgaaaacata tggatttcct ccttattcaa
    30481 atgcttgggt agcttttact gcttttttcc aaccgttgta aagtttttct cggcgctctt
    30541 cctccatttg aacttcaaat agcttgccag cttcataaaa ttcgcggatc tcgtctgtat
    30601 ctttccaata accgacagct aatcctgcta aaaatgctgc ccctagcgcc gttgtttcca
    30661 agttttctgc tcgttggatc ggtacattca agatatctgt ttggaattgc atcagatatt
    30721 cattgtttgc tgcaccgcca tctactttca atacaggaat cttgattcca gtgtcatctt
    30781 gcatcgtatc gatgatatct ctcacttgat aagcaatcga ctgtaaagta gctttaatga
    30841 tatcttcttt tgtcgtacca cgtgtcaagc caaacatagc ccctcgtgct gcctgatccc
    30901 aataaggagc acctagccca acgaatgcag gaacaacata aacctcatct tcgcttgtag
    30961 agcgtttcgc tgcgtcttct gaatcgctag ctttttgcaa catttgaagt ccatctctca
    31021 gccattgaat cgaagaacca gccacaaaaa tacttccttc taaagcatag taaacctttc
    31081 catttatgcc gtaaccaatt gtcgtcaata aattattttt ggataattgc ggtttttcac
    31141 cggtattcat gacaataaat gaacctgtac cataagtatt cttgaccatg cctggttcga
    31201 atgccatttg tccgaataat gcggcttgct ggtctcctgc catccctgaa attggtgctt
    31261 cgcttccata aaaatgataa ccttgcgtat aaccataaac ttctgagtta gaagtgactt
    31321 ttggtaacat cactcgagga atatttagta aatctaaaat ctcctgatcc caatctagct
    31381 gatggatatt gaacaacatc gtccgactgg cattcgagta atcagtaaca tgtgcttgtc
    31441 catcggtcag tttccatact aaccaagaat cgatcgttcc aaacatcaat tcgccttttt
    31501 cagctcgttc ttgcgcgcct tctacatgat caagaatcca gcgtatcttc gttgctgaga
    31561 aataggcatc gatcactaac cctgtttttt cgtggaaaaa ttcttgatag ccttcatttt
    31621 ttaagttatc tgcaattcca gctgactgtc tggattgcca aacaactgca tgatagattg
    31681 gacgtcctgt atctttttcc catattactg ttgtttcacg ctggttcgtt atgccaatcc
    31741 cagcaatctg atttggtttg atccctgatt cgatgaacgc tccggcaata actgactgta
    31801 cagaattcca gatttcattt gcatcatgtt ccacccaact ttcttgcggg aaatattgcg
    31861 tgaactcttt ttgagaactg ccgatgtgtc ttcctttctt gtcaaagata attgctcgtg
    31921 agctagttgt accttggtca atcgccatga tatacgttga ttctgtcatt gtgattccct
    31981 ccattatatg caaacgcttt ctttaactac tcttatcata ctactgtttt aaaaaaagaa
    32041 actgtcaaaa ttttattctt tttttctttt tttgacagtt ttttattatg aagctatttt
    32101 ttaatcaatt cttttctttt ttcagcgtct ctattcttct tttcaatcga ataatgtcgt
    32161 aaggtgacgc aaattctgac agataatatt cacactgttt tttgtttctc tggcaaaatg
    32221 tgatcaccaa atcatattct ttaccttctt cataacttcc aatctcaata ccgccgattc
    32281 ccctcagttc tctgatcaga tattgttgaa aagcaatacg ataaatcggc agactttcta
    32341 aatcgattcc aatcactact ggttttgcaa tataaaaatc gatcatcaaa agaacattgg
    32401 catactggat catcaggtaa ctaagctcac tatcttcagc tcttttcctc ggcaattgct
    32461 gcaccgctgt ttcctgtaat ttcgccagta actgtgttaa ggaatgcccg agcattttct
    32521 gctgctgttc caaaagccga tcacgctcat agacttcgat ctttccaaca aagaaaaacc
    32581 attcattgtt gatctgggaa atttggtagc ctactgcctt ttcttctttg attttcaaac
    32641 gacgaggacg ataatgccat aacatcgttt cccgaatgta agtatctaaa acagcggttg
    32701 gtaacttttt gctccgtgtc aaatcatact gataaaatat ttcttcgtct actatcgaaa
    32761 aagaaacaaa aaagctgtaa aacatcattg tttcgtatac attcagttct gctgctgttc
    32821 gactaagatg catcacgatg attggatcga ttgcttgata aagccggtcc gactgataga
    32881 gtaatttctt ttctttcaaa gttgcgtacg tacttttttc attcaatagt ctttttttcg
    32941 taatcccgag ccagcaagca atctgtgctt ctgctgatgc cgtaaacgtt gtttctaata
    33001 cacggtccaa tcctagaatg aacggtcttt tttctggtgt attttgcaaa aaaagcggtc
    33061 gctgatcttc cgggatatat tggaatagtt cataataaaa atatcgtatc tgtagctctt
    33121 ctccgtataa ttgaccgttt ttgatttgta atccaaattc ctctaagagc tggttcaatt
    33181 ctttgatctt tcgaaaaaca gaagactcac tgatattaaa cgctgtcgct aatcgaacga
    33241 tggatacttg ctgatgttct aataaataga cgagcatctt aaacttcaag ctttcctgca
    33301 ataaaaacaa tagaattttt tccaaactta catcttcgtc caacacaagc agcgcctgat
    33361 actcatttcg ctgtacttct actttttttt ccatcattcg gccaatcaat tcaatatctt
    33421 ccaaatattg atcaaacgag gcttttgaca gttggacatg ctctctcaaa ccattgatag
    33481 aggctgttcc cccttgcaga acaaggtttc gcaaaatcag gatctgtaac tgctcttttc
    33541 tttctaataa tgcttcaatc ttcataggca gtattcgcca cctttgacca caccatatct
    33601 ggcacacggt attctggctg gaaaaatagc tcatgttctt ctaactgttc aaacgtccat
    33661 ccttcttcta ctgcaattgt cagttggtca agaaggtgga acatctcttt tttgctttgg
    33721 agctgtgctc caagaatctt tttttcagaa tcaaatagaa atttgagttt aactttgctt
    33781 tgatttattg ctgtaagctt gtagtctacg tatcctgttt caattttttg gggataaaaa
    33841 atgctctctt cttctgtcaa acctacgctt cctaagaaat agccaaacca ctgattgccg
    33901 actggacgat aggttccctg atcctttgtc tgtgagccac taattgtctt gctgacattt
    33961 cgtgctgtac ggacagcgtt atttactaga gaaacataca ttttttcatt tgttggacga
    34021 aaagttactt ggatcgcatc gccaatcgca taaacgtctt tttgagacgt ttgcaagtag
    34081 tcatctaccc agatcgttcc atcatcattc aatgttaatt gttcttccca catctgattg
    34141 tcgggacgag aatgattagc cagcaaaaca agatcatagt tctcctttct tttttccgtc
    34201 tctacaatag cggtttcatt ttcggtcaaa gacacaactt gttcttctaa aaaaagtgaa
    34261 agcccttgat ttttcatttc ctttactaac ggttctgtca tttcaggatc tagataacga
    34321 aaaagaattg actttcggct ttcgtaaaga tggatttgtt tttttcttga tgccaaccct
    34381 tcagctattt cgattcctgc ttgtcctgca ccaataattg caattttctt tgcattcatg
    34441 atcttttgct gaagagccgt tacgttttgt atctttttaa ctgggtgaat aagcgttgat
    34501 tctgcagaaa tatttcgaaa cttttgattt gaaccagtcg ctagaatcaa acgatcaaaa
    34561 tccaattttt ttcctcctgc taaaacgact tttttttctt tcaatcctat tactgtcttt
    34621 tctgtgtaaa catggattcg atagcggtca gtcagttctt ctttcgtgat ccaagaaagt
    34681 tgtttttcac tggtaacctt tccttttaat atcaaattga acccgctggg aatccatcct
    34741 aacttcgatt gcttttcaat caaatatatt tcacttgttg gcatcaattg gcgaagataa
    34801 atagccgcat gaatccctgc aaacgacccg ccgacaatta caatgttcat ctgcattcct
    34861 ctttcttata aggtgttcta ctgctaactt ctcaatcaga aaacacagta ttcataactc
    34921 gctttcaacc ttatgataca tagcgatgca gcagaatcaa ccctctccat cataccgtgt
    34981 tctaggtctg cttctcaata atgtactagt ggacctaatt gaagagtatc atttctgaag
    35041 agatgtttca aaaaatgtac tcttataaaa gaaaaaaact cgccttttac gtacctctgt
    35101 ccaaatagga aacagaaaag aacgaaaaga cgagttttag cgtgtgtaat gtaatagtta
    35161 gaatagacac atcatttatt tttaattctt taatgcttca acaactgctt cgcctaattg
    35221 atgagcagat tgaggatttt gccctgtaag aagccgccca tcaatttgaa ccacttctga
    35281 aaaagctgct ccagactgat agtttgctcc tttttctttc aaagcatctt ctgctaagaa
    35341 tggtacttca ttcgttgtac catttgcttc ctcttcttca ttactgaacc cagtcactgt
    35401 acgtccagct aaaatagatt ttccagactc atctgtaact ggcaacaatc ctacaacacc
    35461 gtgacaaaca gctgagacca gcccttcatt tcgatagatt tgtgaagcga ctttcccaag
    35521 atctattgct tctggaaaat cccacattgt tccgtggcca ccagcataat aaaccgctgc
    35581 ataatcattt gcatccacat catcaggacg taaggtattt cccaaggccc gatttctgaa
    35641 atcttcatct tcataatagg cccaatcgat ttcatccata ttcaaaacac tttgcggttc
    35701 taaaggtaca tatccgcctt taggactgac aaaatctgca tcgatcccat tttttactaa
    35761 tacatcataa aaatgagtca gttcacttaa ccacaaacct gtaggacgct cgatcttttc
    35821 gtacttttct acatttgtta atacgatcaa tactttcatt ttcccatcct ctttctataa
    35881 aaattgcgtt attgataacg tgctttcaag aaattatata atgggcttgg caaataaagc
    35941 tctttatatg cttcttcaaa aggtttcgtt acaaattcat cctcacggtt ttccaataat
    36001 ttctctgccc agtcaggatc gacgatcatt tgctgtccaa ttgctgcaag ttctgcatat
    36061 ttcaatacct gctctacatc ttcgcgattg cggatcccac cgacaccaac taaagggatc
    36121 tttccttgaa tcgtgttatg gaccacttca agaatcgatt tatcgttgta tttatcagaa
    36181 cgagctattc gatcatacat atttaatgaa acatgcagat aatctagttt tgactcacgt
    36241 aatcgttcaa gaagccagat cgtgtcttca aaacggatac ctggtgtttc atattcttct
    36301 ggtgagaaac gataccctac gatgaatggt tttttagcat attgttcaac cgctgagaaa
    36361 accgttgtta ataattcttc aataaaacga taacgatttt ccaagctacc gccccattga
    36421 tcctctcgtc tattcgaatg cggagagaaa aattgttgga tcaaatacgt atttgctcca
    36481 tgcaactcga ttccatcaaa tcctgattca atcgctctac gtgtggcttt gccaaatgct
    36541 tggattgttt caacgatttc ttcttcactc attgctcgtg gtgtttccgc atcaggacgt
    36601 tttgctgcaa ctgcacttgc agatacaggc tgttctccag ataaagttgc tcgacttgtc
    36661 attcgtccac cgtggaaaat ctgaataatc gcttttgcgc cttctccttg gattgctttc
    36721 gctagctcac ttaatcgagg aatcatttca tcatgagcga tgctgagttc acctggccag
    36781 ccttttccga tatcttggac atttgcagca ccagtaataa cagcaccaag acctttagaa
    36841 cgttgtttat aataagtgat ctcatcttcg gtgatcactc cattgaaaaa gctgagttgt
    36901 gtcgtcatag gcgacagcat caatcggttc ttcaattgaa ctcctgaagg taaagtaatt
    36961 gattgatcaa attgtgacat tgtcattcct cccataattc gctctgatta acctatcgag
    37021 aatcatctat cattaaacca ctcgattagt tgtacatgca actaatttgc ttaaaaaatt
    37081 ttattttttt tgtaacttat ctgttgcatc attcattgtc tgggctaatt ctttacttgc
    37141 ttcatttccc aaaactgctt tgtatgactg atgaagttct tcccagctac catcgacttc
    37201 cttagcaaat tggcgtcctt gttctgtaag ataaactaat gacaagcgac cttcgctaac
    37261 tgttttgacg tatcctttat attctaattt ttcaacaaaa cgagcaatcg ttgatggggc
    37321 ggaatagagc atttgtgcaa tatctttttg tggtattcca tctttttctt ttaatatcaa
    37381 taaaataaaa ccataagttg gtgccagacc tgtccttcta aacgcttcat cagctaattt
    37441 attcaacatg cgatctagct ttttcacgct aaagaaaaga cattcatcta acgctttttc
    37501 aattggcttt tccatttcat cccctccatt ggcgtacata tcatagtatg atttttattt
    37561 tttgtcaaaa aacacggaat aatgtccgcc tcctctgtct agatttgaaa aaaagaagta
    37621 gccatcttca aagaatttcg gcttgttttc gacaaaactc cttctggaac accgtgaatc
    37681 agaggttgtg aaaaagtcgt ttagttttga gtatgaagaa aaattttgtg aaaatggctt
    37741 tccacatttt tgcataaatt tggcttaata ccgaaaaact ggcttttgaa caccgtttat
    37801 atcagaggtt gtgaaaaagc cgtcctgcat caagtaataa gaacagccaa acaaaaatag
    37861 ttcctcatat tttgtcgttt tttgagctaa tgccgatggt cagctgattg tacaccgttt
    37921 attgagaggt cgtgagcctg ccgttgaact ccgagtcaca aggagcaatt tcaaaaaaca
    37981 gcttctcatg ttttatgact atggtgactt gtgatcgagg agttggcagg agaatactgt
    38041 ttattcgtaa agaaggggct gtgacaagac ttttgtcaca gtcccttatt tccttttatt
    38101 ataggatttc gccatttgat tggatgactt gtctgtacca atcaaatgat tttttcttta
    38161 accgtttctt tgtgccattc ccttcgtcat cttgatcaac atagatatat ccataacgtt
    38221 ttgacatttg tgaagtagaa gcgctgacga tatcaatgca gccccacgac gtataaccaa
    38281 ataaatctac tccatctttg atagcttcct tcatctgctc aatatgctgc ttcaaatagt
    38341 caatgcggta atcgtcttga acggttccat cttcttctac tttgtctata gctccgagtc
    38401 cgttttcagc tatgaacagt ggtttttgat acctatcata taattgattc aaagcaattc
    38461 tcaaaccctt aggatcaatc tgccagcccc attcacttga tggaagatac ggatttttaa
    38521 ttcctccagt tattaagttt ccttggaccc cttcccaatt ttctggatga gcactgactg
    38581 cactagacat gtagtagcta aaagaaacaa aatcaacagg gtaggctgct aagatttctt
    38641 catcacctac ttctattttt aagttgacac ctttttcttc aaacattcgt tgcgtatacg
    38701 ctggataata tcctcttgct tgaacatcac taaagaacaa agaagttctt tgctgctcaa
    38761 ttgttttttg aatatcatcc ggatcacaag aatacggaac aagaagctga tagctgatca
    38821 tacaaccgac ttgtgaacct ttgataattt catgacaagc ttttgtagct aaagcacttg
    38881 ctataaactg atggtgtgca ccttggtatt tcgcttgttc caaatgggga tggttttctt
    38941 caattactcc tacgctaaca aaagggtgat gttttacaca gttgatctca ttgaaagtca
    39001 tccaatattt tactttattt ttatagcgtt tgaatactgt ttcagcgaaa cgaacgtaaa
    39061 actgaataag ctttcgatta ggccatccac catattctgt aaccaaatat aacggcattt
    39121 cataatggga aagagaaacg actggctcaa tcccatattt tagacattcg tcaaacactt
    39181 gatcataaaa aaataatccc gcttcattgg gtgtttcttc atcaccatga ggaaaaattc
    39241 ttgtccatgc gatagacatc cgatagcatt taaaacccat ttcagcaaat aaacggatgt
    39301 cttctttaaa gtgatgatag aaatcatttc cgtgtctttt aggataataa tacttgctgt
    39361 tgggatcctg cgccgctttt acttgctgat gcgtcatcat atgccattga tctgtccatt
    39421 tttctttagg taaatcgctg tcgaatgtat acacatcaga cacagataat cctttttcat
    39481 ctacatccca tgcaccttca cattgattgg ccgctgtcgc tccgccccat aaaaatcctt
    39541 ctggaaattt tttcatatcg atccccctaa acttgctagt tttcttgttt gattattttt
    39601 cttatgacta gtaatgatat ttttgaatct actactattt gtaacaataa ccattgtggt
    39661 cggatcatat ccctctttga ttaattcttg caaatctact tccatcaata aatcgccttt
    39721 acgtactttt tttccatcag atactgcact ttgaaagtat ttgccattta aattcactgt
    39781 atcaataccg atatggatca gtatctctgc tccttcttca ctgactaccc caattgcatg
    39841 tttcgttgga aaaactgttg aaacttcccc gtttacagaa ctgtagattt ttccgtccgt
    39901 tggaatgatc gcaaaccctt ctcctaaaga tccactagca aatgttggat cgtttacttc
    39961 agataaatca attatttcac ctgctgcagg agtacttaac actatatcct ttgaggattc
    40021 ttcttcgatt tcatccttgt agccgaaaag aaaagttagc atacaggaac tgatcatcgc
    40081 taagaaataa ctagcaacta gccaaacaaa tccttcacca atataaatcg ggaaagacaa
    40141 aatacttctt gtagcaactg ccaaagcacc ggctcctgcg gcaccagcta ctccaccaaa
    40201 tatagctcca catatacaag caatgtaaaa tggtcgttta tatttaagag tcacaccata
    40261 aacagcaggt tcagtgattc caaataatgc tgtgataaat gcagataaag ataaactttt
    40321 tactttctta tttttcgttt tcaaaaatac cccaagtact gctcctgctt gcgcagctac
    40381 ggcagtcata catgctggac ctagcgtatc acgaccgtag aaagaaagat tgttggtcat
    40441 tactggtaca agtccccaat gtaacccaaa aatcactaac acttgccaaa atccgccgat
    40501 aacagcacca gataaaacag ggctaaagtt ataaacagct aaatatccag ctgcaatcag
    40561 ctgacttgca taatctgcaa ttggacctac aactaataaa ataattggga ccatcacgat
    40621 gatacaaatg aatggtgtca ataaatttcg aatactttcg tgcaagcttt tgcgaatctt
    40681 aggctcaata taagaaagaa cccatacagc taaaattgct ggaataaccg tacttgtata
    40741 ccttgctaga atcacgggca tccctagaaa tcgcaaggtt atgctatcac tatatgcact
    40801 gacaatcgta ggataaacga gagctgctgc aataaccata gcgatgaaac gatctgtatt
    40861 gaactttttt gcagcagtat atgccaaaaa tataggcaag aaatagaaaa atgcatcagc
    40921 tgctgcattc aaaatgatat aagttcctga ttcagtagtt aataaattca aactcgtaca
    40981 taaggcgagt actcctttga tcaaaccact tcctgccaag acgcctaaag tcggggcaaa
    41041 aacagcggcg atcatgtcta taaaagagtt taaaatgcct tttttctcag ttcctctctt
    41101 tccactagat gcatcatctt ctagtttcat ttgtttggat aatacgtcat acacttcatt
    41161 tactgcattt cctactacga cttgaaacat tcctccagct tcgactacag tgatcacacc
    41221 ttctagttgc tctaccttct ccttatctgc cttcttatta tcttttaggg taaagcgtaa
    41281 ccttgttgca caatggtaaa cattgctgac attttcttcg cctccgacaa atcggacaat
    41341 atctttcgct aattgtgaat agtccatttt tacttccccc ttgttaatta atgccgtagc
    41401 aatccttctt acaagataag tatagttctt agaaaacggt ttctccattc aattagtacc
    41461 ccttctaata gcgaacgaaa ctgtacaata gtgtgtatgg tttacttagc cgagcaaatc
    41521 actcactgct tgaaagaaaa caagtatatc gtttctctta tagtcaaagg agacaaaaaa
    41581 aacacgcttg atcttccttt gctgatgtat aagtggtatc tcaagctgat actctgtgat
    41641 ttttgtttct acttggcttt tgataagaca gcacaacaaa gtctcggcat ttaacggaag
    41701 cataactagg tcagaactag gagagattag ttgctgaatg gaatctatct ttttatggat
    41761 ttttatttga ttttcttctt tgtagtaatg atcaagcagt tttgttttct ttttttcttc
    41821 aactatggtc agcttttcac gcaaaatctg ataaatctcc caagattcaa actcatctag
    41881 aacactaggt agataaatca aatcatccca ctcaaaaata agctcttgct cagtagtcaa
    41941 aattgcatca tattccgtta ttttgtttga ttgatttttg aaaagataca ctggttctat
    42001 ccggcattct ttgatccaca tgttcatact ttgttgaatc tgttttttta gtgtattcaa
    42061 aatactaatt ggctggttac taacaatcag tatctcccct tgttttcgaa aaggattcaa
    42121 cgcttcggca agatacaaaa caaaataagc gctttcagct aaattgggac gtaattgaaa
    42181 atgtattggg aagaaacgcc gaattaaata aatttctaaa ggataacggg agatgatctt
    42241 ttgtacaaaa tgattcatgg tattatgtcc ggcatccatc ctcgttttca tttgtttgat
    42301 atgggttagc aaaaattctg tttcatggaa caataacgaa acaggaagat ctaaaaaacg
    42361 aataatcgtt tgctcaaatg ctcttagatt cttctctatt tctttgtttt tttcatcatt
    42421 aatcatcaaa taatttaact ctaatagtcg attttcaatt gcttgttttt cttgattgga
    42481 aaaagaatag ggtagttctg atgataactt ttttgtgagg gtctctacca tcggaataaa
    42541 taatgtatac ctgtttgttt tttctaaaag ttcttctctt cgaagaatgg tcaaaacgag
    42601 ataccgagaa aatcgtatga agtcctctcc tgaaacaata taattatatg atataagaac
    42661 ctcttttaat gtcttttgga cagtcggcat gatattggaa aaaattgtac tgatttgttg
    42721 aggaaacctt gcgtgttgga tcaccgtttc tgttccaaca agagataaga aaattctttt
    42781 tgaattttct tcagcaatga tttttagtcc ttttgtcgac gaaacttcca gttctaattt
    42841 aggattgcgc tcgatccaac gcaaaatcgt ttttatttgt aatgatacgg ctgtttttga
    42901 aagaaagaaa atgtctgcta tcttttgcat actgacatac cctttttgaa acaataagta
    42961 cgtcacaata gttgaagctc ggaaatccat gctggaatag attttatgtt tcgtcacctc
    43021 ttgtatgatt tgctgtttag tttcagattc aattgattct aaaaaataac ctgaacgttt
    43081 atttgactgg ataatgattt tcttctcgta gatagcaagc atatttttga tttcagtttg
    43141 gatcgttttc ttgttggtac ctaaagcttc agctaattct tcgcttgtta tccaatgatt
    43201 cttatttctt aatactacaa gaatttggag ttgtcgttta tttaagtcca tttgcttaca
    43261 cctcctcttt gttataaaca tacaggaaaa acctctcaaa tgacaatagt cacttgcaga
    43321 ggtttaaaaa tcatttgaag aaagatctat attcaaggtt tttaccatta gactggataa
    43381 tttgtttata atattcaaaa ctatcttttt tcaatctcct aaagcttccg ctcccatcat
    43441 tttgtctatc cacataaata aagccatatc gcttcctcat ttcccctgtt cctgccgata
    43501 cgatatcgat tggtccccac cataagtaac caatgatttg gcaaccatct tttattgcct
    43561 cagcaatttc ctgaagatgt atttttagat actcacaacg ttttggatcg tggattcgat
    43621 tcaattcatc tggtgcttca tctagtccaa tgccattttc aactatgaat aatggtaatt
    43681 cataacggtc tgtcaattca ttcatgacat aacgaaaccc tagaggatca attggccaac
    43741 tccaaggttc gggagatttt tccttcaaaa agggattatc gaaaccattt gctccgccag
    43801 tatctacgcg catttctgct tcttttttat agacagcgct acgataataa ctaaatgcaa
    43861 tataatctgc cgtgttagct tgcaagcact gttcgtctcc atctaacatt actggtgcac
    43921 agtcttcttc cttccatgtc ctgtaaatat aagctggata atgccctcgg cacatgacat
    43981 cggagaaaaa ccatgaattg cgtttaaatt gcagtgaacc aaatacattc tctggatcac
    44041 agtcatatgc ataagtcgct aatgcagaaa agctacacat aatacccatc ttagcatttg
    44101 gatcgatttt ctttaataat tgagtagcac gggcgttcgc tagaaacata tgatgacagg
    44161 cttgataaat cacttttttt gttagtgtag caatgggttc aagtttatct ctagcaggga
    44221 atgtaaccaa tccagcagct gcataaggca tacgaaaagc attattgatc tcattaaagg
    44281 tcatccaata tttcactttc ccttggtagc gttcaaagac ggtctgcgca taaaattcaa
    44341 aaaagtcgat taattttcga ttttgccacc ccccatactc tcctaccaat gctaaggggg
    44401 tttcataatg actaagagta attactggct cgatatcata tcgatttaat gtattgatta
    44461 gtctgtcata aaatacgaga ccagcctcat ttggcttctg ttcgtctcct cttggaaata
    44521 ttcgtgccca tgagatgctt gttcgaaaag cttttaaacc taactccttc agtaaacgaa
    44581 tatcctcttc aaatcgatga taaaaatcaa tcgcttcatg agaaagataa tggagtgatt
    44641 cgtctaaagc aatctcccaa atcttttttt cttcattcca ctgaatacta ggatgctggt
    44701 tcatgatccc gataagcgta tctgtcacat taggtaattt gccgtcttcc aaccatgcac
    44761 cttcacattg attagcagct actgctccgc cccataaaaa attttctgga aatatttcct
    44821 gatccataac tatctctcct cgctatcaac aatttcttac ttgaagtata gctcgattca
    44881 tcaaaacaag atattctaac tatttcggca gaagagaaga aaaaaattga atgctactct
    44941 tcatttttaa tatttatata taacaaatac ataagacgat ccccgaaaga aaacactcgt
    45001 taaccatcta agcaaacgat ttattttact tccaacacaa atgcttcata aggtcgtaaa
    45061 tcaactttgc cgtctggtaa ctgtttcaga ggataatttg acacaataac ccttaaagct
    45121 tcacgtggaa tagtgaacgt ctgcttttca ctggacatat tacaaataac gagccattgc
    45181 tggtcttcat atatgcgaga ataggcaaag acatgctcag gcgtcttttc cactaattca
    45241 tagtctcccc agacgatgat gtcatgtttt ctacgcaaat cgatcaattt cttgtacata
    45301 taaaaaacag aatcttgatc tgctaacgct tcttctgcat tgatttcttt ataatttgga
    45361 ttgactcgaa gccatggaat tccagtagta aacccagcat gttcacgggc gttccattgc
    45421 ataggcgtcc tagcattgtc tcttcctttc gccttgattg attccaagat ttcctctttt
    45481 gtaaatccag aagaaatcct ttcgttatac atattgattg tctcaatatc ttttgcttcc
    45541 gcgatatcat cgattgggca attcgtcatg cctaactctt ctccttgata gatataaggg
    45601 gtgcctttca tcatatggag caagatagca aacatcttgg cactttcttt tcgatacaca
    45661 tcgtcatttc cccaacgaga aacaattcgc ggaagatcgt gattattcca aaataaacta
    45721 ttccatcctt catcacctaa agatgtttgc catttggata atactttttt cagttcagag
    45781 atactcaagg gtttcaaatc ccatttgtct ttgccttctt gctgatctaa tcctatgtgt
    45841 tcgaattgga agaccatgga taattcattt cttttaggat tggaataaag tttagcgatt
    45901 tcggtagttg ctccccatgt ttcgccaaca gtcatcagat ttttgtcacc aaaagttgct
    45961 tgattcattt cctgaagata ctcatgcagt ttaggtccat tacttgtgat ttctttatcc
    46021 ggttctttgc caatcatatc gatcacgtcc atacgaaagc ccccaatacc tttatcgatc
    46081 cagaaattca tcatttgata aatctcttgg cgaacctgtt tatttttcca attcaaatca
    46141 ggttgacgct tgctatacaa gtgcagaaaa tattgccccg ttttttcatc caactgccaa
    46201 gcagaaccac tgaatattga gcgcaatcta ttaggctctt ttccattgac cggatcacgc
    46261 caaatataat agtcccgata aggattttct ttatttttac gggcctcaat gaaccacgga
    46321 tgttcatcag aagtatgatt caccaccaga tccatgatga tacggatatt tcttttcttt
    46381 gcttcttcaa tcagttcttc catttcttcc atcgtaccga attctgccat gattgcttga
    46441 taattactga tatcataccc attgtcatca ttcggagact cgtatactgg agaaagccaa
    46501 attgcatcaa tacctaaata cgccagataa tccaacctgg aaataatccc ccgcaaatct
    46561 ccaataccat cttgatttga atcttgaaaa cttcttggat agatttgata aacaactgaa
    46621 tggtgccacc atttttgttc catgattttc ctcctataaa tggatgtttc tattttttgc
    46681 ttaatcattt attttagtct ctctcactaa aaattgtaac gctttcaaat agctattgca
    46741 ccttgttatc ggtaacagaa taaaacaaaa aaacagctga ggaaattccc tcaactgttt
    46801 tttgaaagga ttgatttatg cagggaagaa tgctgatttc acaacttctt tataaacggt
    46861 gttcactcag ctgtcccttg gtattagctc aaaaaacggc aaaatataag aaactatttt
    46921 ttcgtttttt tctctaatac tcaggccaga acgctgattt cacaacctct ttatttcaca
    46981 ttcaatatcc acatgaatcc cacaaataca aagaacagac catacatgat tggagatact
    47041 tcttttcctc ttttagctgc taacatggta attggataga agataaaacc taatgcaatc
    47101 ccatctgaaa tactataagt caaaggcatg ccgatcaaga tcaagaatga agggatcgca
    47161 atctctaagt ttccccattc gatttgtcgc aaagattgtg ccatcaatac tcctaccata
    47221 atcaatgctg gtgcagttac ttgtgaagta acaactgata gtaatggaga aaagaacaag
    47281 cctaacaaga agaaaatccc tgtagtaata gctgtgatac ctgaacgtcc tccaacggcg
    47341 ataccagctg atgattctac aaatgcacca acaggagaag taccaaataa ggaacccgca
    47401 agcatagctg ttgaatcaga agctaaagct ttccctacac gaggcatctt gttgtctttc
    47461 atgaaacctg cttggtttgc taatccaact aaagttcctg ctgtatcaaa gaaagtcaca
    47521 agcaagaatg tcagcactac gacccacatt tgaagagaat tgatgtcccc tacatgtttt
    47581 aatgcaacta aaaatgttgg tttcaaactt ggtgctgcgg caacaatttc agaaggcatt
    47641 ggaatcaaac ctgtgaccag acctaaaatc gttgtagctg ccatcccgat gaagatgcca
    47701 cctggtacac gccgaacaag caagatagca gttacgacta atccaaaaat cgtcaaccaa
    47761 gtagaaccaa cgttgagtga tcctaatcct actaatgtag attcattcga aacgatgatc
    47821 ccaccttcgc tcagaccaag gaaagcgata aatagaccga taccgccaga aatcgcatat
    47881 ttcaaatctg ctgggatcgc atcaatgatc atttcccgca gcttgaagat tgtgatcaaa
    47941 ataaaaatca aagaagcaac gaatacgccg gctaatgctg tttcccatgg aatccccatt
    48001 cctaaacaaa cagaataagc aaaaaaagca ttgatcccaa gagcaggtgc cgttccaatt
    48061 ggataacggg ctaatacccc cattaaaaca cagccgatcg cactggctaa tgcagtcgca
    48121 gtaaacactg ctccttcatc cattccagaa gcacctaata cagttggatt tacaaataag
    48181 atataagcca ttgagataaa tgttgtaaat cctgccagta cctcacgttt taccgtggtg
    48241 ttcaattttt ctatttcgaa gtaggaaagt attttttcct tcatcttcca tccccctaaa
    48301 tccgaacatt ataaataaag tccgttaaaa tttcgattta ttaataaaac aacgtgtttt
    48361 atcatatact aaaacaaaac ataagtaaac aaaaaattcg gattttaagc aaaatccgaa
    48421 cgttccatct gattaaatcc ctttatctca aagttcatga acattcactt tggattacaa
    48481 gaatttcttt attgtgttaa taaagtttct tcattctagt ctcaaaaatc aatagtgtga
    48541 tttgatttta ccatttcgtt tctgttcttc tttgatgaat tcttttgtat cgacaggatt
    48601 cagattattt agtccgccaa aacctgtatc gaccattttt ccccagcctt ttgtcttctt
    48661 ttttcctgat ttgagattct ttgctcgaag cttctcacta tgttttgctc gccgatactt
    48721 caaataacct tctaatcttc ccattagatc cactgttttt cctccttttt attcaatcag
    48781 acgactcagc ttttcctata acaaaataat tgccttcagg gtcagaaaaa ttgaaagtca
    48841 agatgccatt gttatctgtg atttctcctg gttcttccat gttctcgtag atagtttcaa
    48901 attcatccgt aaaaaatact aaagaaggcg gtggtcctaa tacctctggt gaaaacttct
    48961 gcacaaatac ttttgggaat acgcctaatt ctaattcttt tgagatgctg agtacaatac
    49021 tcttaaattc ttctggcaat tcgattgttt cagaaatttc tgcaccaatt ttttctacaa
    49081 agaatcggca aatcatctct acgtcattga cataaagcat cacacgtgtt ttttgtatca
    49141 tcatttgacc cttctttcta tttgattttc tttcctgtct ataaactgat tccattatat
    49201 cacgaaagcg caaacacctt gtgtttgata ctattcaaga aaaataaagc aatagtttct
    49261 aggattgctt tcctccacaa acttttttta caaaaaaaaa acggcaatga atgccgctct
    49321 tcttatttcg attcttttat aattctgctt ctgattcatc tttggttagt gtgtgtctaa
    49381 cactagttac gccaaatgtt tgatcgattt tttccttcaa cgcttcgatt gcctgtgcgc
    49441 cacgtgccgc ttgttcagca ttgatggatt caatgaccag caccgctgct tgtgtttctt
    49501 ccgtcagcat cgttctttgg gcttcacgcc agttgagaca gcgacagatc gctccttgtt
    49561 catcatagta gatgatttct tccggaagtg ccggagcatc ggaatctgca cctaaaggca
    49621 ggaatgattc gccaccttgg gcttttccta aatgcagatt tcctgcgatc ttttcgatat
    49681 cttctccgcc gcaaggtacg ccaaaagaca aggaaatact attatagata tctacaagcg
    49741 gattgattgg gaaaaattcc cgttcttgat ttgcacgttt cagtaaagct tcaatagaag
    49801 aacgtgcacc ttttttagtc ttgaatgtgg taaatgcctg acgccactga gcaatgactt
    49861 cgttttggct gaatgtttct tctgtaaggt aatttcttgc ttcttttgct gcctcattca
    49921 ataattgatg atacgtttct tcactttctt ttgttacgtg gttatcgata ccatttacgg
    49981 ataagatttc gatggttgct tcaggaaata cttcccagaa attggtatcc actattactt
    50041 ttttcatctg ttcacacctc ttgattagaa taattgcttg atgaatagat agtctgtttg
    50101 gggatcatct cccatataaa aaacatgttc gctgtatttg ataaatcctt ttttacgata
    50161 aaaagcgagc gcaacatcgt tgtgttccca aacaccgagc caaatggctg ttttttgctt
    50221 ttcttttgct ttttcaatag ctgtatctag taaaaatgta ccgatacctt gatgtttaaa
    50281 tttttttcgt acatagattc gctcgatttc taaaaaatga tcagccattt cctcagattg
    50341 actcgttcct gtatttagtt tcaggtaacc cgcaggctct tgatcgatac tcactaggaa
    50401 aaaatcggtt tctttatctt ttagctcttc tgtaagtttt tctagtgcat aagcttgtgt
    50461 caagtaatca gctaaatgct ccggtgaatt atcttttcca aatgtatctg taaatgtttc
    50521 gatactaatt tgttgtagta agtctacatc tgaaggcgtc atttttcgaa tttcgtattt
    50581 attcacggct tcttcctttc aatagatcct tttattgcct tttttgacaa aattccaatc
    50641 ttcgctaaca ttcttgcata ctttatttaa aagatattcc aattgttttg cttcttcgtc
    50701 agaaagacca tttaatgcga ctgcattgga gtagttgttt tctcgaatga tgaaaggata
    50761 gatttctgct ccttttttag tcggatagag tttcttgatt tttttatttt ctttatcttc
    50821 caaacgttcg atcatcccat tggattctaa tttcttgatt gctcgggcag tcgttgtacg
    50881 atctactttg atcatttctg ctagtttttc ttggatgatt ccaggatttt cgcaaatacg
    50941 tactaaataa agatactgtc ctcttgttaa atcgacttct ttgaattcga tattgctaat
    51001 agaatctagc gctcgggcaa tcattccgat gtctcgaagg atttcagcca tttcatccca
    51061 cctttatttt gtttgtaaga ctagttcacc tctttttgtt gcaaatgcaa ctattttcaa
    51121 taaaaaaatc aagttctttt ttaataacta gtaaagtata ttcttttctt cttttacgaa
    51181 aaagctcaat cacaatagat ctggtttttg ttttctaaaa taagtaactg tttcttcgtt
    51241 gttcgctttt ctgatagctt tggcaactct tctatactaa aaaatcccat gttggaagtt
    51301 tcattattct caataaactt ggcctcttca ccgtggatcg cacaagcaaa aacaagttta
    51361 taatactgaa aaagctgtgg aatatctttt tgtttattcg tatcaaaaac tgctcgcaac
    51421 tgagtagttt ctactgttaa tcctgtttct tcatagactt ctttacgcac attttcttca
    51481 ggagacaatc ctatttcagc aaatccacca ggaagtgacc attctttcgt ccgtttatct
    51541 tcgattagta ggaccttgcc atcttttttg atataggcac gaacatctac ttttggtgtt
    51601 ggatgtcctt ctccaatcgt tgtgagcgct tttatttctt cgaaagtttc ttttcctatt
    51661 tcactgatca gttccaatga aatcgtccgg atctcctcaa aacgctcttt atcaaataca
    51721 tcttttccat aatatagacc agcttgagaa agtgcgagta gtcttttgta ttgagccaac
    51781 ttcttgttca ttctcttccc ttctttttta gcaaatgtat gactatgtaa tcaagtatag
    51841 aagactggtt taattacggc aaataaatat aatgaaatct gcaataaata aaagaatgtt
    51901 aaaaaactcg agaacaaaag acattcgact tctctggggc tgtgaataaa tggcgttcaa
    51961 aagcttgcat ctgcggtaat aagccccacc aaacgaaaaa tatgacgagc tattcttgtc
    52021 tggctgttct tattaattga ggcaggacag ctttttcaca acctccccac taaacggtgt
    52081 tctcctacca actcctcggt cacaagtcac aatattcatc aaacatgaga agctgttttt
    52141 ttgaaattgc tccttgtgac tcggagctga acgacaggct cacgacctct cggtaaacag
    52201 cgttcaaaag ccaatttttc gatattaagc catttctttg ttacttattg tcattgattt
    52261 ctttttctaa tttatccaat agtggagtgt aatccacttg agtatagtct gttacatcaa
    52321 attcatatag tgttcctaac tggaatttgt catattttcg gtcggcgatg atctttttga
    52381 atgcttcttt cgtaatatga ttggtccctt tcgttcgatc ttccaatacg tcaccatagt
    52441 gataatgatc catgatatag ctcaaatgac gatctgattc acgatcacgt ttgtaccgac
    52501 gttgccataa cacttcaaag tctgctgtta ggcgaatcgt gatcacctga tatccgtatc
    52561 tatctgctaa ttcttggagt cgtgcttttt gcttatcact aaaaggatac tcggacacaa
    52621 tcactttctt tcctgcttcc atatacaaat caagtgcttt gtaatagaaa ggataaactt
    52681 tcgtcctttc ttgttccgcc cgttcttcca aattgttaaa cccgatggat tctgcatagt
    52741 attccttgat ttcatcgagc gtgatggcat acatatctgg aaatcgttcc cgtagtttat
    52801 tcataagata tgtttttcct gtaccaggac taccagccag aagaatgaaa tactttttca
    52861 tctcatttat ttcctaagtt aattggactt tccaagttca taaacagtcc aatcttatgc
    52921 ttgacaattt cttttactgc ttcatttgct ggaatcacat tatggcgaag agacttgttg
    52981 atttctgttt cactaaaggt ttctttagct actttcgtcc agttgactaa aagctccgtt
    53041 ccaacattga atttgcggat cccgttattg atcaattcag gataatcttc ttctttcaca
    53101 cctgttcctc catggataac tagggggaca tcaactactg catcgatttc tttcagcaag
    53161 ggaatgttta cttctgtttt tgatttgaat tgtccatgat tcgtaccgat agcaatagct
    53221 aatgcatcca cgcctgtagc ttctacaaat ttcactgcgt cttccggtct tgtatatacc
    53281 ttgtcatttt cagcgacatg gattccttct tctgtaccgc cgattgttcc aagttctcct
    53341 tctacggaaa caccgtgcgc atgggcatat tctactactg cttttgtctt caggatattt
    53401 tctttgaaag gcagatggga cccatcatac atgacagaag tatatccgct gtcgattgct
    53461 tctttgatat catcaaagtc tcgggcgtgg tctaagtgca agactgcatc aaccatttct
    53521 tcttctgcaa tagctgtcat tacattgact aacacatgca tgccgatata tttagcggta
    53581 tcgacacttg tttgaataat gatcggcgaa cccatttctt ttgctgcgcc tagcatagca
    53641 ggaagcattt ctaggttatg cgtattaaaa gctccaactg taaaattcaa ctcttctgct
    53701 gtttttgtta cttctttcaa tgttgtatac atcttttatt gccaccttat ctttttattt
    53761 gtttcctttg atcgttactt gttttgcaat ttgtcgcatc tcatccattt tgcccatgta
    53821 gtaatcgatc ccttgatcat caccagcacg agcagcatct gcacgcttct ggttatagag
    53881 gctcatcagt tttttatacc catctccgat cgtctgttta ttttccaaac tcttttctaa
    53941 atattggatg gcttgatcgt tcacatacaa ttcagaaaat ttaagtccaa cttgttcata
    54001 aagtttcgct aatttatgct tatcttgggt agtggaaatc tcttgtttca attgtgtaat
    54061 ctctgattga atcttttcgg tcgcttctgg cgatagggct ttttcttctt ttgccagaac
    54121 ttttttcggt tctttttctt ttttcttaaa aaatccaaac atcattttcc ctactttcaa
    54181 ttaaaatgct ttcaaaactg ggcttaagat ccaggcatat aagagtgctg cggctactgt
    54241 atctacatcg gttgctgtag cattgttgaa tcccatcgaa ttaaagatcg ttactagcaa
    54301 agcgggcaat aatgtaataa agaaaccatg agcaataccg ccaatgatcg ctcctcggcg
    54361 tccgccaact gcgttgccaa atatccctgc tgtccctccg gcaaagaaat tggttagcat
    54421 accaggtaaa atcatcgcta agccgaacat gggcaaagta aacatggcaa taatcgtacc
    54481 aatcgttgtc gtaatgaaac ctaaaatgac agcatttggg ctataaggaa agaaaactgg
    54541 acaatctaag gcagggatcg catcaggaac taatttcata gctatccctc gaaatgccgg
    54601 cacgatttca cctaacagta gacgtacacc agctagcaat acatacacac ccactacgaa
    54661 ttggatggct tgtaaaaagg caaacatgat atagttttga tctcctgata attcagaagc
    54721 gaatggttcg cctgcaaata aaacagtgat gatatatagc gggaccatca cgaccatgac
    54781 agacaaataa gtatcttgca gaaattcgaa atgtgtcggc aacttgatat cttcgatcga
    54841 ctttttattt tcgcctttct caccaaaaag cttagcaaca ccggcttcaa ataagtaccc
    54901 gatcgtacaa aaatgaccta aagcaatatc atttgatcca gtgattttac gaatgatcgg
    54961 ttgcgctact gccggcatag caatggcgaa acaagctccg ataaaaccac cgaccaagat
    55021 caatacgatc cctcgcagac cggcaaaata gccaaagact gtcgtcatcg ttgccatcca
    55081 taaaattgct tgaccagtta aaaaaatgta cttccatttt gtgaaacgag ccaaaataat
    55141 attgaataca aaaatggcta agaacgtcag tgcaatatct ctacctaaac ccaaatcatt
    55201 cattgcttga ccattgatag attcaataga agggatgatc ccttgcatat gaaatccttc
    55261 agtaaagatt ttcccgaaat aagttaaact tccaacgatg atgctcgaac cagcactcaa
    55321 cacttgaaat cctaggagtg tctttaacgt gccagagatg acttgtcctg ctgatttctt
    55381 ctgcagtagt agacccaaca tagcaatcaa tgcaatggtg atcgatgcct gagtcaaaat
    55441 attttcgatg ataaaattga taacgcccat gtttttaccc tcttttcttt ttgtatttag
    55501 ataactccct tatcttgcag aactggggtg attttttctt caatttcatt ttttgaaaca
    55561 atatttttca aatagatcat cgccgtttta ggatcaactg cgaacttttc gaactgcgaa
    55621 cgaaaatttt ctgctgtgat gatcacatct ggtttcatgg aaactgcgga agaaatatca
    55681 caatggtcca gtttggcttc tactcctaat ttgttcaaaa catcctcagc agacatctgt
    55741 gctgcaaaac tacttcctaa acctgcaccg catacgaata aaatgttcaa tttgttcatc
    55801 tttgttctcc cccgtatcat ttattatttc tcaactactg aaaaaagcag ttgtttgaaa
    55861 tcttcgatat ttgtgcattc aatcaatcga tccaacttct cttcgttgtt gatcaattca
    55921 attagttctt tcatgatgtt cagatgcgag taggagtcta ctgctgccaa acaaaaaata
    55981 atctttactg gatccatttc aggattaccg aaatcgattg gttgctcaat cgtagctacg
    56041 ctgactccta atttattgac cccatcttct ggtcgggcat gggctagtgc catgtgtttg
    56101 ccaatcacga tatacggtcc gtaatcattc actgcacgaa tcatcgcttc gacatagcgc
    56161 tcttcgacaa tgttttcttt aaccaatggt ctagcagcta gttcaatcga ttcctcccaa
    56221 gtagtggctt tttctttgat catgatattg ctgtccgtca aaatatcttt taacataggc
    56281 tggatctccc ttttgttgat ttctaaattg tttgtttcga ataatttttc taacttgcca
    56341 tagatgattc gatctactcg gccaccactt tctttcatga catctattat ttgagtgaat
    56401 aactccgtcg attcatgact gttaggctct aaccgctgta gttcttcatt tcttttcaaa
    56461 aagtcttcta taaccaagat attcgtctca gttaaaatcg gatcgacaac taaaagcggc
    56521 ttatttcgat aattcaattg aaaggtgctg aaaaccagat tgacatccaa tttatctact
    56581 aattcgattt cttttgaact taaaactgca gtaatctcga tctgaggaaa cttttctttc
    56641 agattctctg ctaacagatt tccagtagcc atcccatggt tacagacaac gactgattta
    56701 taaacataat tcaagtctcg cttgattaca ctggcgatcg tcgaaaagtg gatgacaaga
    56761 aaagctatct cgtcctccag tatcttgcca ccaattactt cttcgatcgt aggaacaaaa
    56821 tgagtgactg cgcggtagat tgggaaataa tttgttcgga tgttttcttt caatggattc
    56881 atgacttgga tatgattctt gattcttaca atcagtgcag ccatgtgctt atagagattt
    56941 tcgaataaag cttcttcctt tagatggaaa ggtacgtgtg tctcgttctc tacaaattgg
    57001 atcaattgga tcgttagtaa ttgagcttgt acccactcga ttgaattgga gatatctcta
    57061 gcattgaacg tctcaatagt gaaacgaaga taatttcttt ctacttgcgg gacattgtcc
    57121 aattgaaatt cctcgatcat gtggtcaata aatgcagaaa agctactgtc ttttatttct
    57181 gtatttttcc aattaatggc agagataaat tcatgtttcc tgattcgctg gatccagatc
    57241 aatgtaaaaa gcaggatttg ttttttataa atatcatctt cttgtttcaa caacttattg
    57301 gaatagatct cctgtatttt tttgatttcc accaaaggaa aataatggag aaagattttt
    57361 tgagccggtg ttaattgtgc atcttgattc ataaagaagt cggtcattcc cagattctga
    57421 ttgataaaat catagatcat cgtacggata gatcgttctg aacctttata aaccagtcct
    57481 tgctttccat aactgacgat ctctatccca taactgacca gtgttgctcg taaccgtctc
    57541 atatcttcat ctaatgtact tttagagatt tgaaattctt cttctttttt gtttagatgg
    57601 atcgggattc ggctgtaagc aacggctaaa actagatcaa aaatacgttc gcgccgattc
    57661 aaaaagccgt ctttcacatc ttttatttca gtttctaatc gagcttgctt ttccttttcc
    57721 agcttcaaca aaaaaccttt gttgcgaatt gtacggatct ccggaagctt tttactgctc
    57781 aaccagatat tcgcttcaag aacttcctga cggatcgtcc gctgactgac attgaactca
    57841 cttgctagat gttttgcttt tactggatat tgggctgtca tgattctgtg aagtatttca
    57901 attgttcttg ttttcataga aatgatctcc ttctgatttc aagtatatgg ttccgcttac
    57961 ataataaaaa gtagatattt ctgccacttg aagtggaaag aaagtaagca aagcaaacaa
    58021 aaaataagcc atatgctttt tcatctcctc aacgaaaaag acatacgcct attattacaa
    58081 aaattttgcc catctataca aaaaagaggt tgtgaaaaag ctgtcctgca tcaagtaata
    58141 agaacaacta aactaaaata gtgcttccta tttttcgttt ggttgttctt attatcgcag
    58201 atgcaagctt ttaaacacca tttagtggag aggttgtgac agagatgttc agccttgagc
    58261 gataagaagc gattttgaaa aatcgcttcc catattttac aaaatcgtgc cttattgccg
    58321 ataggctatc tctggcacac cgtttattca gaaaaaggag ctgcgacaag gcttttgtca
    58381 caactccgtt cataaatatt tcctgtcaat ccagcataat cggctgaata aaatcggctt
    58441 gccatctttt ttctttctca gaaaaatcaa aataccatat ctgagaaaaa tactgtcgtt
    58501 gtgtcttact tcctgagatc aatcgcccat cttttcgatc gactgaataa tcacagcatc
    58561 tgaaatcaat tcggatggca aaactatttt cgtggatctg tcgataattt cctacttcct
    58621 gaatagttat ttttttagta tgatttctta caccgtctgc tttgtttttt tgcagaactt
    58681 tctgatgttc ggcaaacagc ttgtctgtat aacagcttct ggctttttca ggagtttctt
    58741 cttcccaagc ggattggatc ttcatgaata tcttttggat ttccagaaac tgtgctttct
    58801 tttctttagc cgttcctgac atgcgggaca atatctccgc tttcagcctt ttcttttgtc
    58861 ttttattctt gacgatagaa aaagcagaaa cccctcctac tgacaaaaca gctaatgcaa
    58921 gaaatcccgt ttgctcataa ctagaaggac ctgtacctgg accataatac ccataagaat
    58981 ctctgccgta agaacgactg ccaaaataac tgcccccata tgaacgacca ccaaatgaac
    59041 ctctgcccga acttcgaccg ctagatgtac ctccaccatg accacccgcg acagcgaatc
    59101 cttgggtcgg cagagcaaac aaacataggc ccactaacaa aatgattgct aaataattga
    59161 tttttttcat tgtcttcctc gttatctttt attacaaaaa actattttta tgatagcctt
    59221 gctaaaagga agagtcaata accagatgca taaactgttt ttttagcaat tttgtcttat
    59281 ttagtcattg agtaacatcg gacaaatgta tgtgtcggca gtccataatt cctctatctt
    59341 cagcatgaac aagcttgcag acaagagatc tgcgcttcct ccaggagaga tttggttttc
    59401 ctgacaaaag tgcaacagtg ctgtaaatgg acgatttggt aaagcacttt gactagcagc
    59461 atgagcaagt ttttgcattt ccactaacgt tttctgatca ctgcgatgca atacgcatgt
    59521 gtcatctagc gaactcatca aaactaacaa catatgcatt tgtttctgta catatgaatc
    59581 attatctagc tccttagctg cttgaattgc aagcctgata tgcggatacc ctaatatcgc
    59641 ttcttcatat gcaccattta cgccatattt ttgcttcacc ctatgaccat gagtggtttt
    59701 tttatgacga tattttgtat caggaaaagc agcaagcttg ctgacatcat caagtatttt
    59761 cgataaagaa aaaggctgtt ccatactgat tttttgtgca atgacactga tcaatagccc
    59821 cattgcccaa atcgctcctt tatgggtatt gatgccattt gtagcaatca gcattgcctt
    59881 ttccgctttt cgcccaatct cagcaattct ttctcttaac tcctgatcaa tagcatgatg
    59941 ccatgctatt tgagccattt cttcaaaaaa aggagttagc gtcttagcag acttaagaaa
    60001 taaagcatag tccatatcgg aatgtgcacc cgcatccagt gcatccacta atccaggttt
    60061 tggacttaat gttacttctg cgatcaatgc ttcctctacc aacgcagcga gttgctgagt
    60121 cactgctttt tttcgttcgt catttatcat gacactcact ccttcttcct actattactt
    60181 gctgaaacct tttttgatag ctacccaaat gatatgaagt aaccagctta aagcaaatgc
    60241 acaacttgcc gtcattagat aagctacaag aatcatgact cctgaaggta actgcagcca
    60301 actatcttta aacaataata acagtacttg gaaaacaaat acattggcta aatacgtacg
    60361 ataagcataa acagacaacc atttgaacaa tggcaatttt ggcgaatcgt ttacaatcat
    60421 tttggaagcg cctatgaaga cagcaaagat gatgactaaa ctgtataaac tttggatcgt
    60481 attcaagtaa ggtgcattcg caaagctcaa gtctcctgga tgtgcgagca atgaacggac
    60541 agaaaagaac gcgataacta aggcaacagg taagaaaaga taacgtactc tgtccaaata
    60601 ttgatagact ttttcgtgat aaacaaatgc ggctacccca taaatcatga aactagcaac
    60661 aaaccgatct aataagtacc agctgtcatg ataaggccca gtcaatactt gtgtttgata
    60721 gaacagatac catgcgacat ataaaatcgt tgaaccaatc aatactggcc atacatattt
    60781 tttattagca aatactttgt aaccgagcca aacaaaaaac ggcatgaaca attggatctg
    60841 cagcatcata acggtatacc ataaatgagg cgctccatca cctgtgacat atttcagtaa
    60901 aaagcttgca gtatccgtat acggagtttg ttgttggact gctggaaata gccaaagata
    60961 agccgtcgtc catagaagat acggaacgac tagttcaaaa aacttttctt tcaaaaattc
    61021 ccaataagac aaatgctgac ttgtttttac catattataa acaattgcaa aaataaacat
    61081 tggtgctgtg tatttaacga tgacgtaaaa ggtaccaaga aaagctgtat ttgttgcgct
    61141 gtggctgttt gccaaagcaa agctcaatat cgtttgcatc ataacagccg ttacagctga
    61201 tactttctgg aagtcactta cgttgatttt gatttttttg ttttcaaaca tctctttctc
    61261 tctctttttc tttattacca aagttctttg gttaatttag gtccttcgac tgttttcaat
    61321 agaatttttt tctctggctc tctcaaatac tcttttagtg aaaaaccgcc ttgccgagtc
    61381 actacttggg tatcgaattg ctcaaaaaaa gcaggatgcg attgaattac ctgaattggc
    61441 aattcaatcg gtgaatttgc gtacagcagc aaatcaaaat cactggtttc ttttactgcc
    61501 ggaatcccag tcgataattc aaatcctaag ctgccgccga ttccccattt acatcctgat
    61561 aatatgtttc tagcagcatg aaattgctga taaacaggat aatccatgat tttctcttgt
    61621 ctatacgatt cttgttcgat gatgtcccac ggatgaatga cttgtttgac ggcatgtttt
    61681 gagatttctg ttgaccatct ctgattgcgg gcaggtccac gtaaaccaat cggtagtatt
    61741 tctgcgtgat ccgtcgacgc acgacgaact gttccgaaca tcttttcagt tagaacgacc
    61801 cattcaggca aggtgaaccc ttctatttca tgtttatcaa aaaggacaat atcatgtgct
    61861 tgaatcattt tttatgcatc ccattcttcg tccattttgg cacggacttt atttgtttcg
    61921 acacgtccga atgttttggc tgtttcgttt gtgtatcgat ttgtcagcat cgtgtcattt
    61981 gtattgcgga catcttgaat ggcttcattg attgcttcga tgactttgct gacattttgt
    62041 tctgaagcag cttggtcggt gatatcttct aggaaatgat agatcgctcc tagttttgta
    62101 taattattga tatcgtaagc gatagctgga actttttcag cagctgcttc gatttcagca
    62161 accgaacgtt ttgtcacacg tgcagcactt tctttagaca tcgcttggac ggtaatttct
    62221 ttattgttta aagcaatcaa gcggcttgat tgtaatccat gtgctaaaaa tgcacctgaa
    62281 atagcatttc ctgcaatcaa tccaataact ggatgtccag cttgacggag ttttgcataa
    62341 gcagcagcac tattagccaa agccacatgg ataccgatca attcttcgtt atagccgtat
    62401 gcttggcttg gtacatcgat cacaacgatg attggccgtt tcactttttt gtctttatct
    62461 tcttcgtaaa cgtgatccaa aatttgtccc atgcggaaac cttcaactaa accaacttca
    62521 ccgtttcgta cacggtacat cgggttttta ggatcaggta cgattgatgt cacgatggct
    62581 tcttctatga atttacttga tttaccataa tacacagtac taatagaaga agttggattt
    62641 ttcatgccag ttagtgccgc aaaccaatcg taaccaacac ttgctgcagc tggttcattt
    62701 ccttctgcta cttctggtaa gtcaattgat tttgcttgat ggttagcaat tgcttcattg
    62761 taatcgtcaa tggccattgg ttttgaaaga tctaaagaat caagtaatga taaataaaat
    62821 gctgagcttt cactgcgatg cgtgtctttt cgttcgtcca ttgctgcgat gacagcagct
    62881 ttgattgctt ctacactatc agtcactaat tcatcaatga tttttgattc tacccgttga
    62941 cgagatccta atgtattcca aatcaagtct ttgtcactag aatcaaattc tcgaacgcct
    63001 gcttcttgtt caataacttc tggtccattc aaaccgactc tcgctttttt tgttgcgatt
    63061 aaataactca ttaatgctga agtaatcgac ataccgccaa atgatcctac acgtcctgga
    63121 atcaaaccga tgactggtac atattgtttt agagcaatga ttacatttcc gatttctgaa
    63181 atagataaca gtccgtagtt tgcttcttgc aaacgaacac cacctgtatc tagtacaatg
    63241 actggaaaga tctcatttcc ttttttattt tcttctaaca catgttcaag tgcagcaacg
    63301 atttttgcac cggaaacttc accgataccg ccaccttgga attttccttc catagcgatt
    63361 actactgctt gtttgccatt gatcgtgcca cggctgacaa cgatcccgtc atcactttca
    63421 gggacgattc cttgtgctgg taaacttggc gcgatgattt gatcgaatgg atcaatcaat
    63481 tcacgtccat tgtcaagtag agcgttcact cgttctctgg cgtttaattc tacaaaacta
    63541 tttttcatct gaaagttcct ccattgcttg cgtcaatcgt aggtacacaa ctcctggtgt
    63601 agcaccgaag tcattgatca cgatatctgc tgaaattgga taacgatcaa aaaatctcgt
    63661 taatacattg ttccatactt cgccgaagcc atcgcttcct gtacaaattt ttacagtaga
    63721 ttctgttttt tctgttggaa acatcaagat ttccaaatct cctgaaccga ccactcctac
    63781 atggattttc ttattgatcg gctgtttcgt tggatattta taagctaatt gttccatgga
    63841 tctctcccct ttttgtaaaa cagagcatac acgttctgct tattcgtctt tcttcttcca
    63901 tctgggtaaa tggcgacaaa aggtttttct cgccaatttt atgaccaagt tctgaatttc
    63961 attggtggtt catacaggcc atctgaccat tcgatcaaat catcgatggt ttgtgcagct
    64021 aataatgaac gtttcgcttc actacgtttg atgcctagat cttctggtaa tttcacgatg
    64081 ccggctgcac gcaagctatc tagtgattct ttcgtgctag tcattccaag aggtgtaaca
    64141 ccgccaattg ctgcaattgc tgcttgtcgt tcttcttttg tttttgcttt gtacaaataa
    64201 gcaattcctt cttctgtcac gatatgtgta gtatcttcgc tataaatcat cactggtgca
    64261 ttggctaagc ctgcttgttt ttgaacagct agtgcatcta attcttcgac aaagactggt
    64321 tttttatttg aaccatatgt ttcaaccatt tgaacaacta atttttggcc tttccctaat
    64381 ggatcatcat tgtcgcgaag tgattgccat gctggtgtag aatgacgacg accgccagga
    64441 ttatgtccca tgttaggcgc accgccaaaa ccagataaac gtccttttgt tacggtagaa
    64501 gagtttcctt cgtaatctaa ttgcaaggtt gaaccgataa acatatcgat ggcatattgt
    64561 cctgcagctt gtgataatgt tcggttactg cgcatcgtgc cgtctttacc gacaaaaaag
    64621 acatctgggc gagcagcaat atatttttcc ataccaactt cgccaccaaa actatggatg
    64681 ctttcaaccc aacctgattc gatggctgga atcatcgttg gatgtgggtt caatacccag
    64741 ttcttcgcga ttttcccttt taaccctagt gattcaccat aagttggcag taacaattcg
    64801 atagctgctg tattgaatcc aataccgtga ttcaaagatt gtacgccgtg ttctgcgtag
    64861 attcctttga ttgccatcat tgccattaga atttgcaatt ctgtgatgtt ttgaggatct
    64921 cgtgtgaata aagcttctaa ttgataaggt ttatctgctt tcacgacaac gtctacccag
    64981 tcaccaggga tatctacacg tggtaaagaa tcgacaactt cgttaacttg aacgatcacg
    65041 ataccatctt tgaaagctgc agcttcgatg attgttggtg tttcttccgt attgaaacct
    65101 gtgtacaagt tgccgtcttt atctgcttta tctgcagcta ctaaaacaac atttggaatc
    65161 aaatcgatga ataagcgtcc ataaagttcc aaataagtat ggatatcacc taatttcatt
    65221 gtgccgtctt caatcatttg ggcgacacgc aagctttgtg caccagcata agagaaatcg
    65281 atcttgcttg cgattccttt ttcaaaaata ttcaaatgtt ctggtcgtga gatactagac
    65341 atgatcaaat gcagatcatt cactttttct gcatctactt tttcaagact ttctgaaaga
    65401 aaacttgctt gtttttgatt gtcgccttct aatacaactt tgtcgccggg agcaatcagt
    65461 ttttccatta cttctacgat tttttcagtt tctacatact tgccattgag caagttgctg
    65521 attttttcca atcgctgcgc ttttttttcg cgtttagaag cccagttgcg gtttggtttt
    65581 tccataatta gtcatctcca tttttgatat agaaacgtgg cgtcatcaat accatgttgg
    65641 tctagattga tcgtacggat ccctttatct acaccaaatt ttgtatgaat cagctttgtc
    65701 aaagtattgc cggggggaaa ttcaatcgtc agatccattc ctaattcttt tgccaagtcc
    65761 atcatttggt tccactgcac agggaaagct aggttttcca acaaatcttt acgaatctct
    65821 tctgcgtcct gagtggctct tccatccgta ttttttatat acaaacatct agcatcagat
    65881 agatggacag actgcatata cggcttgaat ttcttgaccg tatcattcat caagagacag
    65941 tgagatggca cagggacttt cagcagcttc gctgtttgcg cttggtgccg tttggcttct
    66001 gccaacgtaa cggataaacc ttcgatactc ccagataacg tatgttgcat cggacaattt
    66061 tcatttgaca gataaactgg atggtcagga tcaaatgttt ttgctacaat ttcctcagct
    66121 tcgcttctcg tcacacctac tactacgccc attgcataac cgcttggata agcttctttc
    66181 attaagactg cgcggtgata gactagttcc gctgcattct caaatctcaa agatcgacaa
    66241 gcaacagctg cacaaaaagc accaatcgaa tgtcctgctg caatatcagg ctgaataccg
    66301 tttttttcca tttcatctaa ataaaatact gcttttgtca gtaatgctag ctggataaaa
    66361 accgtatctt gatagttttc ttctttatca actaaccgat accccgtcac attttctaaa
    66421 cgttccatgt acatttcagg gatatccgct aacatcccta acttctgact gccttgtcca
    66481 ggaaataaga aacctgtact catgagcctc gcctcctcag cacttacttt ttccactgct
    66541 atcttagtca atttctgcta taataaaaaa tgccaaaaaa agaaaaaacc ataacaaaaa
    66601 ggtatggatg ggatggataa ttatgaaatt gcttcatctg cagtatttta aaaaagtatt
    66661 cgaaacaaaa aatgttaccc aagcagccaa agaattattt atttctcaac ctgccttgag
    66721 ccgtgcgatc aaacatttgg aaaacgaatt aggtgtccct ttgttttacc atagcggcag
    66781 aaacattgag acgaccgttt atgcagaaga attttatcca tatgcagtca agacattaga
    66841 cacattagaa gaaggaatca aattattgtc ttcggtcaat gagaagatcg ttacttccgt
    66901 catattgtat ttagaggtag cctctgtttc tattccgaac ttggtccgaa tttttatgga
    66961 aaagcatcct gatatccaat taacgatcat gcaacatcat ttaccagatg atattaccga
    67021 tcctgtactg tacatcactt cggagatgaa gcctggcatg accaatattc ctatcatgaa
    67081 agaacctgtt tatgtcgcca ttcctaagaa acatcctctt gctaaaaaag aacatctgca
    67141 attagaagat atccaacgca cccccatctt gatgctgtca aagagcaacg ccttccgtca
    67201 tacactggat gcagcacttg aagcgaaaag tatcgaattg acgatcagtt cgacgacaga
    67261 tgatcctgcg acattacgtt ctattgtcaa gcaaggatta gctgttagct ttttcccaaa
    67321 agtttcttgg tcttacgata aaaatgatcc tttcgtcttg cgggaaatca aagatttgcc
    67381 cttgacacga acaatttatt tatcttctag cttcacagaa gatcatcctt tgatcaaaat
    67441 gattgcaaaa acgatgcggg aattttattt aggaaaataa aaaaaaaaga gctgggacaa
    67501 aattcttgtc gcagctcttt tttcgaacaa acggtgttcc agaagtaatt tcttagaaaa
    67561 taagccgaaa tttcccaaaa atttgagaag caattttcgt aaattccttc ttatttcttg
    67621 aagctaaaca tttctgtcac aacctcttct ctattgtcct agacattcat tgcgttgatt
    67681 taaaacttcc atcaatcaaa tctgctagtt catctagctt ttctatttta cgtagatcaa
    67741 tagagatcca atgttcttta ttcatgtgat aggctggaaa atacccctct tttcctcgca
    67801 aaattccgcc aagttcagga tcgaccttca aatcaagaat attgatgatc ctatggtcac
    67861 ttgcatcatc atagattttt tcttctggaa cagacatcag gaccccaaac cattttttgc
    67921 ttttttgatg tctgaatacg caataatcag gaaatttttt aaaaatatat tctggagaca
    67981 aattgtatgt ttcattgatg tgatcgatta gttctttttt cgttagcatc tgcttccatc
    68041 tctttcttat tgattcgctt tattcaaaga taccacgtaa gaatagaaaa atgaatacaa
    68101 gtagttgcta atcaaatact tcgatatttt tcttttttat caactcttaa atgttcaatc
    68161 cgcctgtgtt ggtcttgcta ccatctacta ttaaaatgtt tattattgtt tgcccaccta
    68221 ttttttgatt ttagtggtat catttttcaa gttcatgatg aaattcttat catcagtaaa
    68281 tgctccagaa aaataaacaa tgattgaaaa atcagttgaa acttgaccac cgtttatttt
    68341 ctccgtattc ttagaataga agggagtctg attgatgtat tcactaatga aaaaaatcat
    68401 taccgaaaaa gatattcgac gtcatgtttc tttagtagaa caattgctga atcatacaaa
    68461 gataaccgta aatgaattag ctgaaatcat agggacaacc gaacgaacga ttttttctga
    68521 ccttcaatct attcgctctc agctgcccga aggctgggac atcatcagcg atcaagcagg
    68581 aatcagtttg caaaaccagc aaaatttatt aacaaatgac ctttgggaga tttttttcaa
    68641 acaatcagtc agtgtcgaac tgctgaaaaa tctgcttttt acaaaaaaag ttgctgtccc
    68701 tgattttcta gccgaccatg ggctttctta tgggacattg aaaagacatg tgactaaaat
    68761 caatcagcgt ttagcaagtt acgacttgca gatcgatttg acgaaatata cagcttgtat
    68821 tcttggaaaa gagcgtgcga ttcgtacttt ttatcatcga ttgctgattc ctttcactca
    68881 taacaactat tttttcgagg attattcgat ccatgaatcc cattattttc aatttttaag
    68941 aaacctcagt caaactgaac tagcggttga aacagaagag attttcggaa catgttggtt
    69001 ctttatcaat acgatccgta taaaagcgaa ttgccgatta gattcttcta tccataccaa
    69061 cagcacactc tcctccctgt atgactctgc attgaaaaaa ttgtatttaa cggaaggtat
    69121 ttatcttaaa gacactgagc tttcttttgc ttctttctgc tttctggaaa gttggaatta
    69181 caacaataac tatggacaag aaaccgctcg ctgcctgcat cattctccat ttttagaggt
    69241 cctcgaaacg tttgtagagg aactagcttc agagctgtct cttgatcaac taaaaaaaac
    69301 atctctgact gataacttgg ctctcctcat attaaaatat catgaatcgc cgatattgat
    69361 tgaagcattg gaccgtcaat accatcgttt tttagaaaca tatgatcagc aatatgcaca
    69421 cttgtatcaa cagaaagaga atttactgaa cgatctaaaa aaagaaatag aaatcaatga
    69481 accaaattac tttctccaac tcttgtctct actgatccaa caaacgattt tttctatcaa
    69541 accgaatttg ttcaatgtct attttatttt ccaaggagaa ccttcatgga aggcgttctt
    69601 gcagcaggaa ttaaaagatt acttaggaaa acgtgtctgc ttcttaccta tcgaactaac
    69661 cgatctttcc actatttctt ttgaaaaaac agatatcttg atttccaatt tccctctaga
    69721 ttctctagat atccctattg tatacatttc ttctattcca acgaaaaatg agctcaatcg
    69781 tttgactgaa ctcactttca aaactttttt atagtaacgg ctagcgactg tattctctag
    69841 ctgttttttc ttttttgcga aacagtttgt tcatgatccc tttttctatt tctacaacta
    69901 agaatagaag aatcacattt gctccaatca ataactgttg tgtcatcgtt aaacttactg
    69961 taccgatcag ctgctggccg actggtagaa aaataaccat cccttgcaat aaagcaagga
    70021 tacctaatga aagaaaaagc gctttgtttt gcaaaagacc ttggttcaat gacggcttgg
    70081 acaactctct gcaattgatc atgtaaacag cttgagctaa gacgatattt tgcagaagaa
    70141 tggtttgctg taagctttgc ccttcaaact gcatagccaa taaataggca ggaaccatga
    70201 tcagtaggga gacatagatg atccgaaaca aatgataacc agataggatt ccttgctttg
    70261 gatctcttgg tgggcgcttc atgatttctg gatctggcga ctcgaaccct agtgcataag
    70321 ataaagtgat tgtcgttacc atattgaccc ataaaatctg tactggggac aaaggtagcg
    70381 ggcgattcag cataagtgcc caaatcaaga tcaatccttg agctaatgca gttggcaaga
    70441 agaaattgat ggttttttgc aaattatcca aaatccgccg gccctctttt acggcttttg
    70501 caatcgtatg gaaattatca tctcctaaaa ccatatcagc tgcctgtttg cttacttcac
    70561 tacctttgat tcccatagca attcctacat cggatctttt caaagcaggt gcatcattta
    70621 cgccgtctcc ggtcattccg acgatttctc catttttctg caaagcttta acaattcgca
    70681 gtttatgttc tggtgtcgtt cgagcaaaga catcgactat tggcacttgc tgaatcaact
    70741 cttcatcaga cataagatcc aaatcgatcc cttctaaaac tttggcagta tgttttaagc
    70801 caatttgttc cgcaatcgct tttgctgtgt ctttgtgatc ccctgtaatc attttcactg
    70861 agatccctgc ttgttggcat tcctttacag ctttgactgc actttctttt ggcggatcga
    70921 tcaatccggc gatccctgca aaagttaatt gggttaaagt ctcatgcgtg agttcctgtt
    70981 tcgaatctac ttttttataa gcaaatccta aaacacgctg accttttctc gcaaattctg
    71041 cagctgtctg actccatgct tcttgttctg cagaagaaag agttgattta gcaaataata
    71101 cttctggtgc acctttgacg taaatcaccg ctcctttttc ctccgcatgc atcgttgcca
    71161 tatatttgta ttcagaactg aacggaatct ttgcttctat ttttttcaaa ggtagattgg
    71221 ctgtttctgt gtgtcgaatc agcgcaactt ctgtcggatt gccacttaaa ttttctgttt
    71281 tctgttcttc ttttgttttt aattcctgac agtttttcat aatggacaac acgcaggctt
    71341 cttcttttgc agctacatcc acaactgtca tttcattttt tgtcaatgtt cctgtcttat
    71401 ctgaacagat cactgtcata gaacctaatg tttctaccga aggaagtcct ttgatgatcg
    71461 ccttttcttt tgccatttca tgtactccaa cggatagaat catggtcaaa acagctggca
    71521 atccttcggg taccattgac accaccaaag caatcgtagc cgaaagaaga atatgccaat
    71581 ccataccata tcgaaatgtc gtaaaaataa ctaagaatag gctcaaacaa agaatgcctt
    71641 gaaaaatttg tttgtttaat cggttgattt ttttaatcag aggcgtcgtt tgttgttcga
    71701 ccgattgcaa tgcttgattg attttaccga tttctgtcgt gtttcctgtt tctacaacga
    71761 caccgactgc agaaccagat tgtacaagcg ttcctgaaaa acccatattt ttctggtcgc
    71821 cgatcgattc ttcttctgtc aaaacggcat gacttttttc agatgcttct gattctcccg
    71881 tcaaaatcga ttcttccact aataaatcat gtgcttcgat taatcggata tctgctggaa
    71941 cgacatctcc ggcacgtaag acaatgacat ctccttttac cagttctttc gcagggattt
    72001 tttcttgcat cccgtttgat agtactatcg cttcttgtcc catcagctgc ttcaaaccat
    72061 tcaatgattc ttccgctttt ctttcttgcc agtaactaac gagtccattg acgatcacta
    72121 cgaataaaat aatacttcct tcaatatatt cactagaagc aaatttcaaa aaagaggcaa
    72181 tgatcaacac gatcatcagc agatcagtaa aatgctttgc tatttttttc catcgttttg
    72241 tctgctgcct tgcttttatt tcattatatc cttgatcagt caggcgtttc tgtcgttctt
    72301 cttgcgacaa gccttctgtt gatgtttgat agtttttcaa tacatcttct accgtcaatc
    72361 tgtaccagtt catcctatcc cctcctgttt aattgatact gttattatac gcagggaagt
    72421 tttttcaggc gtatcgatta tttcaggggg gtctgaaata attcgttttg caaaatgtat
    72481 ctgggcctaa ctgatatttc tattgcttat tcttttatat acaaaattta agaatacaaa
    72541 ccagataacc aataaaaaag gaataaagcg tgtcgtgctt tcgaataaca agctgaaagc
    72601 aattgaagta aaaaacacca acaaagcaag atctttatat ctttgtgtag tgttccgttt
    72661 cttttcttta ataaattgaa catgtgcaca caagatcgaa gaccacacaa atagaaaaca
    72721 actcgttgtc atgcctgcca aaagctgaaa aaacaatcga ctatcagcta caaaaaaatg
    72781 tagacaaaga ccggcaaaaa ataacattcc tgtaattaaa ataccgtgta aaggtacgcc
    72841 tttcttagaa agcgcacaaa accaatgggg caaacttttt tcttttgctg tttgatataa
    72901 aatcctgcta gtactgtaga ctgcactatt tgctgaggac aaagaagcaa ccaaaatcat
    72961 tagattgaca attgctgctc cgccttggca atggatcact tgcagcatag tcactaaagg
    73021 actttctttt aaatctaaac aatgccatgg aacaacaaat aaaatcgaca aaatcgttcc
    73081 tatatagaaa aagagaatac gccaaggcag acagttgatt gcttttttca aattcttttg
    73141 cggatcactt gtctcgccaa cggttaagcc gatcatttca attccagcaa aactgaataa
    73201 aaccatagga aatgagacta ccattccagc aaagccaaac ggtagtaatt catcgatctg
    73261 tccaaaattt tctttgattc cgatagactg ggtatgaaaa tctgagacac tcaatcctat
    73321 acccaataag ataaaaagga aaatccccaa gatcttgatg attgagaaaa aagattccaa
    73381 ttcaccaaac cacttgactg caacaaggtt gactgccatt aagatcatta gtgcgctacc
    73441 tccgcttatc aaagctggta catgtacaaa ccagaaatgg atatacaatc ctattgctgt
    73501 taattccgca agtccagctc caagccagca aagccaataa gtccaattga caaaaaatgc
    73561 ccactttttg cctaaataat agtcaacgaa atcactgata gagttaaatg tactatttgc
    73621 taaaaataaa tcacccatgg cccgcatcaa accataaata gccaaaccgc atatcaaata
    73681 aacgagaata acagctggcc ctgctgatgc aattgccttt gctgaaccta aaaataatcc
    73741 tgttccaatt gcgccaccta atgcgattaa ctggatattt cgattagtta gagtccgttt
    73801 cattgccggt ttttccatca agttcacctt tttctttttc aagcagcatg gcgacttcct
    73861 gttcccaaga aggtttcttt attttttcaa tacagagtgg tactacaaac aaaatgacac
    73921 tgatggctgt gactgcaatt agattgatcc atgttccagc tttcatcgtc agtccgacag
    73981 aaaatccgat tgccgcaaat aaaacaataa cgacagtcca aactccaaga ttgctctttt
    74041 tctctttttt tccaattcta aatggtcgat tcaactttgc atgtgtataa cgcaatctca
    74101 aaactgcgat ccccatgatg acatagacga tacagtagat aacagtagtt gaatttacga
    74161 gcatcaaaaa tgcttgattg acgccaggaa tcagcaaata aaaaagtgca aagattgaga
    74221 taatgacaga ctgagtcaaa ataacggatt tggatacgtc aaattgattc gtcttccaaa
    74281 aattgaattt tggtggatac gcaccttttc tagcactttc tgtcactgtc tttgctggtc
    74341 ctgaggccca agcagataat tggactgcta caccaatgaa gacaagtaaa ctaaacagat
    74401 tcacaataag atgtggcaaa ccaagaattt ggcaataaat cgaaatagat tgcgcgatat
    74461 tattcaattc catttgtcct tttggtacga catttgctac aactaaagca ttcaaaatat
    74521 tgacgaaaaa tataaaaatt aacgcggcaa atactccgcg aatatacgtt ttaactggat
    74581 tttccaatcg agtgatatac acagaggaca tttcgatacc agtaaaaata aacatgatcg
    74641 gcgcaaaata aacaaatgag gatgcagtcg tagtatctgg tattaatttg tcccatgaaa
    74701 aactgcctaa tgttccggtt ggtacgatcc caactttgat ccacgcagca aaccctaata
    74761 agagcatcat cgtcaaaggc acgtacaatc ctaaccaaac accgactttt ccgccgattt
    74821 ttgccatatc aaatttcaaa ttcaaaaaag tgatgatcca ataaacgact aaaatgacaa
    74881 ctaatgtaaa tttgctgttc aaaccaagcg gcacattatc gataatatta ccaaacaacg
    74941 gagcgagtgc tgaagcgacc atcaccatcc caggaaacat ttggacccat aacagccatg
    75001 agacaacgaa cccccatttt tttcctagag cattcgatac ccaaagttct ggaccacctt
    75061 tttggggaaa cattccagca aattcaccag agatcaatga gattggaaaa gcgtacaata
    75121 atactgcaac cagcatgtaa aaaatcatcg tccagctcgt tgctgctaca tctggaatat
    75181 ttcgtacact tgctacaagg gcgcaggtca ttccgataaa ggtcatggtc gtgatttgct
    75241 ttgatgaacc agtatctttc atctttattc cttctttcaa tagcgacata tgtttttgtt
    75301 ttcttttatt tttgttcaag aacagaaatg acaaggtttc atcgattcag tacaaaaccc
    75361 agtatcaccg aaacatccat tgaacgatct tagtgaactg ggtttgatac atgaaacgaa
    75421 cgatccaact tatagaacgt tttataaaaa agaatatctt ggcgaggaat atatgaaaaa
    75481 cagggggaaa tacaattgtt ttgatgaagc aaataattgt ctgttctcca tatgtctctt
    75541 gttattcttt cacacaaaaa tcagttctat tggatgattt cgtttagttt cttttccatt
    75601 gctttggcga ttttttttgt ataaaaattg aaaattcgat catcattcaa ataaggagtc
    75661 atgatagccg cacgtagcag agtgattttc tgttcttttt tccattcttc tttcgttaga
    75721 cccattcttt caacaaaacg aatcggagaa tcaccgtaat cttcttgtgt aaatgtagta
    75781 tgagaagtaa taaaccgttc accataaaca tcgccatcca tataagaaga ccgatcgaac
    75841 attttttcat ttaattcatt gatagcgttc aaatctgtac agccttgttc tttgaatacc
    75901 caattgacca tattaaaatc aggatgattc aatggataga cttcgattgt ttttccttta
    75961 acagtaaaag tcagatgatc gagaaattct ctgaatcttt gagctgcctc gattgaagcc
    76021 ccaatcaatt gaccatatcc ggtcacattc aatggcaata cacgatgtgc tgtccagact
    76081 gcggcggcgg ttgctccagc ttttgatcct tccaaaatat aagctcctaa catatctgga
    76141 gcctttactg atttttcaaa cacatatggc gcgaaataag aaatgatatt tctcatattt
    76201 ttatgtttga ttacgattcc tccagcagca taagggacat agcccatctt atgaggatct
    76261 atcgtgacag aatccgcttc tgaaatagct ctgaaccctt catatacctc tttgtcaatc
    76321 gttacgcagt ggtggaaaac atgatgttct tcaaagaatt cagctaagga agcatacggc
    76381 acaaattctc ctgcttcgtt tagaaacagt gaacgcgcat agccgccata tgcggcatca
    76441 acatgtaaat agaaatagat cccttcatct tttaaccttt ctcttaattg gacgatttta
    76501 tctacactgt ctacttgacc ttcttctgtc gttcctacga cagcaaccac acctagaatt
    76561 gggatttttt gtccagctaa ttcacgaatt gttttttcca aagcattaat atccattcga
    76621 taatcctctt gaactgggat agcaaccatc tgatctaagc cgactccgca aatatctaac
    76681 gccttcatcc aagaatagtg ttttgtttga gggaccagcc atttccctaa tctttggata
    76741 ttttttccgc tacgggaaga agctgctttg acctcatcca tttcttcgtc cgtaaagctt
    76801 tcggtcattt ccaaaatttc ttcaacagat aaattcaaaa gttcccactc agacattttt
    76861 ttcacttttt caggcagcac ttccttgaca gctaaaggaa tcgatttgat gcaacgggcg
    76921 taccataatc cttcaagatt agcaatagaa ccatctgctg caatgtgtcc ccatccgtct
    76981 gtcatattga ataatgaagc aaaatcctgt cctacttctg cttccatttg cgaagttgcc
    77041 atagaagatt ccaatgcaac attatttggg ttccacagca tcgcatagtt gtaagctaat
    77101 aaagcaggca tcaatgtttc agcattcatt tgtccccagt aacggcctgc tgaatgccat
    77161 gggatcgatc ctgcgcgtaa tcgctgactg acttcatcca agacctctat catatggtcg
    77221 cgggtggcta gataatctgg agtgagctta tctccttcgc taattgcagg catatctgaa
    77281 ggaatataat tttctctcca tccaagatgt tcatctacca tcttgttcaa caacatttta
    77341 taaactggtc cattttcagc tttgtcgcct ataaagacgg ccttgatatc catatctttc
    77401 atttttcttc cctccatatt ttgttggtca atgtcttgca gatacatgtg tttatctgta
    77461 acacctcttc actatagcgc caaaaatgag aatttttttt ttataacatc aaatccacgt
    77521 agatttttac aaacaaaaat aaataaagtc ggatttttct gataattcaa cgcaaaaaag
    77581 cgaacaattt ttcaataaat cagtcttcta aaatagataa cctaaaaaaa gcgaacaaac
    77641 aaataaaatg actgtccttc attcactaaa catattttta tttttttata ttgttaaaaa
    77701 ttcgcaattt attttttaat agaattatct gttttccaaa ttttataaca taaaaaacct
    77761 tttttaacgg catttatatg ctttttcaga aattaactga tgatttcaaa aagaaaacat
    77821 gtcataatat ttcccatatt gtgcaaaaaa atacaaggcg gaggctctac tatgattgaa
    77881 gaatatattg agaaaaatat tttaagacag ctgtttttat gtgggcaatt ctatgtaaat
    77941 aaagaagtca atctagaaaa gctctccaat ctcctgcatg tttgcaaaac cactttactc
    78001 aatgatatca ataacataaa aaaagaattt gaagaacaga tcgcttatac acatcgggaa
    78061 aaggaccgtt acactttata tttttcagaa catatacctc gatgcaagat catgcagcaa
    78121 ttgtctcaaa attcgttgtt tttaaaaact tgtctgcttt atttggaaga agatgaacct
    78181 gattatcttc aattaactga atgtgaattc atctctgtca gcaaagctta ttcattaaaa
    78241 aaacaagtat tagcttattt taatgactgc gggatcgaaa tcgaccgcta ctcccctcga
    78301 ttcactgaaa tggaaagacg tctgctgctt cttaatgtgt cgtatcgttt aggcggtttt
    78361 aattcttggg agctacctga atcgtttttt gaacgtgccg atcgtttcat tgaatccgta
    78421 acggaaaata gtggcagatt ttatgataaa gaaaataaag aaatcttatc gattggtttt
    78481 gctatcagtt tcctacgcca gcaagtgtgt gctgtgacaa tagattcaaa atttatcgaa
    78541 gaaatcaaaa aacgtcctat ttacaattat gtcgaatcag catgggaaaa cactgatttc
    78601 caaacctatt ataaaaaaga agaattcgcc tttattttga cactattcaa tctttgtaat
    78661 tatggtttcc attcttatca gttgattgcg gaagactttc aacagctaca ccaagtgttt
    78721 attgacaata ctcccgaaat aaaagaacta gttgctacct ttgaatctca cttcaatcaa
    78781 gaattattcg gaaaccaacc gttcgaacgt gcgctgattc atctgatgcg ttcggcttgg
    78841 gataactatc aattattcat gccagaaaaa ttttatcttt taaatgagga acagacaaat
    78901 ttatataaag aagtccagac aattttttcc agctggtcca gccagttacc ttatgatcta
    78961 cggttgaatc ctaattgcat gcgggctttt gtgatcgaat tgagcggaat tctccgtctg
    79021 acaaaagaac atctgactat ctatatcgtc acaaattcgg atgttcatta tctgatttat
    79081 cgggaagcct tggaagctgt aactactttc gattttcaag ttgcaccaac aatttattca
    79141 agtatatctg atataaagaa gtatgcgcag cagtcctcaa accgggtatt atgtgaacgt
    79201 acattatata caccagatgc ggtccaatat gaaaatatta ttcctatttc gatcaatacg
    79261 atcgatcgag ctatcatttc agctgtgcaa aacaaataag gggctgtgac aaaaatcatg
    79321 tcacagcctc ttatttgttg aagatgccca tttctgtaac aatctcttga tacacgtcag
    79381 gctcacaacc tcacttattt tggacatcta cgggtccgat ctctttattt aaaaaataag
    79441 aaatgatggt tcggataact accaacgccg ctagttggaa aatatcctgc aaggtaggtt
    79501 tgataatcga ttcgatgatc ccagctgaaa caagaatttc caaacttaga agtatatagc
    79561 ctcccaacat ctttttaata gcattgttct cgctgactcg gtcgctttgc acttttgtat
    79621 ggacaaataa atatttcaaa aaatccttga ttgatagaca aacaccccaa accaataaac
    79681 agatagcaaa gatatcaagt cccagtacaa gtttttcgaa atatggaaga agaaactgca
    79741 tcaactctgt cgtcatccta cctctcctct tctttgtttt tctttgtctt ctatttcctg
    79801 attcaagaaa taagaaatga ctgttcgaat gataataata agtgcaagct tcaaaatatc
    79861 tgtccaagtc ggcttgataa ctgactcgat gatatcagca acgactagta cttccaagct
    79921 taacaaaata taacttgcta gcagttttct tgtatctgca ttacgagtat tacgttctgc
    79981 atagtttttc ttgacatgag accaatcaaa aagtttgaat agaacaagaa cgatcccgac
    80041 aattaagata ccgatagaac aaatatttaa aatagcaatg atccattcta gaaattcaat
    80101 taaatttgag cgaaacatat acgctaccta ctttctttta atttgttcct attcttttat
    80161 tgtaaaagaa agttaccttg ttcgtctatt taaaagttgt tctaatgata acggttcgct
    80221 taaatttttc tttttcactc tcccattttt ctccctccat ccataaatcg tggaagtatc
    80281 caaaacaacg tagcaagtta aaataaggct tagtccgtca tcagcttttc aaagaagcac
    80341 tgtctcggtg ctttttttgt acccaaattt ctaaaaatag aaccatacac ctcctcaaat
    80401 cttttcttct tacctaccct atttttattt acaggaaaat cagacacttc ttcttttttg
    80461 tcaaaatttc ctttttcttc tcgtcctatc atttggcatt ctaataaaaa cgaactatcc
    80521 ttggatatag aaagagaaaa caaggaggcg tttttcatga gtaaaaaatt aatcaaagtc
    80581 ggcatcggtt taggtttgct tgcccttggc gctgcatatc taggaaaaaa gacaggacta
    80641 ttcgaagatg acagtcattt atatgatgaa ttcgaatcaa tctagcatcc tatttagttg
    80701 aatcactacg gacatacttg gaagacattg gaggaatcac aatgagaaaa agtaaagcaa
    80761 taatgattgg tgccggtatt gccaatatgg cagcggctgt ttatttgatt caagaaggca
    80821 aatggaaagg ggatcagatc accttttatt cattggatga tcacggttca aatgatggtg
    80881 caccgaccga cactgtagtg gatgaatatt ggaataaaaa ccatccatta gaaaaccaaa
    80941 aaggctatat tgctcgaggc ggtcgcatgc ttaattaccg tacctatgta gatttgatgg
    81001 atctgttaag tcgaatacct tctgttactg agccagggat gactgctgaa gaagatacga
    81061 gagattttga tgcaaaacac cgaacctttg acaaagcacg tttgatggaa ggtggcaaag
    81121 gaatcattga tggcggaaaa ctgggattca ataataaaga ccgtattctt ctgactaaac
    81181 tgattgctat gcctgattca gaagaagaaa aactagacaa tatcactatc gaagaatatt
    81241 tcaaagatga tccacacttt tttgaaacaa acttctggtt tatgtgggaa acaacatttg
    81301 ctttcagaac acgtagttct gctcaagaat tgcgtcgcta tatgcatcaa atgatttatg
    81361 aattcactca aatcgaacat ctagtgggcg tcaatcgaac acgttacaat caatacgaaa
    81421 gtattatgct tcctttgatc gagtacttga aagaacaagg atgcaaaatc attttgaacc
    81481 gccgtgtcac tgactgggaa ttcaaagaaa caccgatgca agatgaaatc actgtgactg
    81541 gtttgaaaat taccaataca ctgactcaag aagaagaata cgttgtggtc gatgaagata
    81601 cagcagttat tttcactaat gggtccatta cagattcagc tactttagga gattacgaaa
    81661 caccagctgt agaaaatatg gattatggtg cagcttctag tctctggaaa aaagccagtg
    81721 aacgtttcta caacttggga aatcctgaca aattctttgc tgatcgagat gcaagcgaat
    81781 gggtcagctt taccctgacg acgaaaaacc atttgttgtt gaatgaaatc acccgtatca
    81841 cgactcaaga accaggaaat gccttgaatt catttatttc aaccacaccg atcacgccgt
    81901 taggccaaaa agatgtcaat atgtctatcg tcgtgcatca tcaaccgcat tttacgtctc
    81961 agaaaccaaa tgaaacagtt atttggggtt atttccttta tccaagacga cgtggcgaat
    82021 ttgtggataa acagtatatt aaaatgaatg gaaaagaaat gctagaagaa ctgattggac
    82081 aactttcaaa agtagatcct ggaccagtga atattcgtga aaaagaaaca gaaattttcg
    82141 acagcgtaat aaataatatc cctgtctata tgccttatgc ttccgcgttg ttcaataatc
    82201 gtgccaaaag cgatcgacca aaagtcattc ctaaacattc gaccaatctg gcctttacag
    82261 gagaatttgt cgagcagcct tatcagatga tctttactga acaaagtgct gtacgttctg
    82321 gagaaatcgc tgctttccat tttgcgggta ttcctatgtc ccgtcttgtc aaaacaccgc
    82381 gttacgacaa agatattcct actctagctc gtgccgcaaa aaaaatgttt gaataatcaa
    82441 gaggttgtga aaaagctgcc ctgcatcaag taataagaac aaccaggcta aaacagcttc
    82501 ttatgttttt gatttggttg gacttattac cgcagatgca agcttttgaa caccgtttac
    82561 caagaggttg tgaaaaagct gccctgcatc aagtaataag aacacccaag ctaaaactgc
    82621 ttcttatgtt tttgacttgg ttggacttat taccgcagat gcaagctttt gaacaccatt
    82681 taccaagagg ttatgaaaaa gctgccctgc atcaagtaat aagagcagcc aagctaaaac
    82741 agcttcttat gtttttgact tggttagact tattaccgca gatgcaagct tttgaacacc
    82801 gtttaccaag aggttatgaa aaaggcgttt agtttcgagt atgaagaaaa attttgtgaa
    82861 aatggttcct cacgtttttg cataaatttg acttaatacc gagaaactga tcccttctgt
    82921 cataacctct ttttatttat gtctgtttct ttttgaaaac aactattttt tcaacgttta
    82981 ctctcttgcc aatccaacat catctaacgg atacaactat ttcaatgatt accattacac
    83041 taaaaaatcg gaaacacacc tctccgaaca caaatacata agactataga atgtttcatt
    83101 taagaaaaaa agcgtttcat tatcaaaaag gtaagatctc ctttaaaatg aaattatctg
    83161 tagtactaat aatgcagaca attttatgaa tctgaaagga gcatctccaa tgaaagcagt
    83221 cacttggcaa ggaaaacaga aaatggaaat cagaaacgta gatgatccaa aaatattaga
    83281 accaacagat gcaatcatcc gtatcacagc aacagccatt tgcggctccg atctgcatct
    83341 ttaccatcat ggtgaagcag ttttggaaaa agggtatgtt gtaggacacg aaccaatggg
    83401 aatcgttgaa gaagtgggat ctcaagttca aacattgaaa aaagggcaac gtgtggttat
    83461 cccttttaat attagttgtg gtcattgtca tttctgcctg aatcacatgg aaagccaatg
    83521 tgatgaatca aatccagatc agttatatgg cgggcttttc ggatttggca agttgaacgg
    83581 taatcattgg ggtggtcaag cggaatatct aagagttcct tatgcagact ctacttcatt
    83641 tgttgtacca gataatgatc ttcctgatga aaaagtttta tttctatcag atattttgcc
    83701 cacagcttat tggagcgttg aaaacgcagg agtaaaaaaa ggcgatacag ttgtgattct
    83761 aggttctggt ccagtcggtc tattcgctca aaaattcgct gttatggctg gagcagagcg
    83821 cgtaatcgca gtagatccag tacagcaccg tctagataaa gcggctaatt acaatggggt
    83881 tgaaacttat ttcttagaag acacggcaca agctggaaca gaattgtatg aactggctaa
    83941 aggtggcgca gatgtcatta tcgattgtgt aggaatggac ggattagagc ctgtcaaaga
    84001 aaaagcgaaa aatttagtaa gcttacaaag cggcacaatc tctccattac agatggcatc
    84061 gcaagctgtc aaaaaatttg gaacagtcca agtaactggt gtttatatga ctccagcttc
    84121 ttcttacccg ctgcaagaat tttttatgcg ggatatcgct gtcaagcatg gacaggcacc
    84181 aattatccat ctaatgccaa aaatttacgc aatgattgca gaaggactat tcgatcctac
    84241 acaaatcatt actcattcgc tgccactatc agaagcagca caagcatatg agatctttga
    84301 caaaaaagaa gataaaaata taaaagttgt tttgaaacct tgaattcagt aaaaaataca
    84361 ggtgattttc tttatgccca aaatcaaacg aaatacccat gagatcgaaa taggaaaaga
    84421 agaagatatc gaaatacgag gacgtcgctt ccgcttctat ttccaaaatc gttacacatt
    84481 gatctcactg gctgtcgatt tattaaccgg gattttctat atttttggaa gccttgcttc
    84541 cttaactaaa atccccgatc gatatggcac ctatttttat ttggctggtg ctattttctt
    84601 gacgattcgt cctattctca gaatcgtaag gaacattttt atttacgacg aaaaaaaagc
    84661 aatgaagaga acaagcaaaa aaaaagaggc atgagaattt ttcaaatctc atgcctcttt
    84721 tttcacccta tcgtatcact ttgatacaga cgagcggtcg tcccactttc ttccttgacc
    84781 attgtcaaaa atgtccactc gtatcgttca taaaacgtgg tatgatctgt aattaagtaa
    84841 agtttttcgt acccaagttg atgcatttct tttctgattt ccgctaatat ccgcttggct
    84901 atcccctgct tacgcttttc cggttcaaca aacagggcac aaagatttgg tgtcagatca
    84961 gggcggttat ggaagtcgtt ttcaatgacg cccgctcctc cgacgatggt ttctttttca
    85021 tctaagacca cataccattg aggaatactt tccttattag cgattccagt acgaatactt
    85081 tcctcataca caacagcagg aactccccat tttgatgaaa accaattcgc gctttgtttt
    85141 actaattctg ggtgttctct taatttgatc cagttatatg taaccaatgt ttaaacctcc
    85201 gactaaaata aaacgtttac aaaaattata caagtcttta tgaaaattag caatgacaac
    85261 aatagtgatg taaaaaaaaa acggaaaaca atcacttgtt ctccgttttt tgattaatca
    85321 agcatctaaa gcttcttttc ttctttcttg gatcgtagct accttgacga acggtagata
    85381 gatcaaaata gaaagaacca aattgacagc ggctagtaca ccacctgcaa tactttttgt
    85441 tgccaataca ccgccaataa ttggtggagt tacccatgga ggcataacgg tagctgctgg
    85501 cactaaacct gtagaagtcg caaagtaagc aacactaact aagaccattg gtgtcaaaat
    85561 aaatggaatg aacatgatcg ggttcaacac gataggtaac ccaaacatcg ttggttcatt
    85621 aatattgaag acacctggcg caatcgacaa gtttgttaca accatatatg gtttgttttt
    85681 acgtcctact aaataaatag ctaacagtaa acctaaagtt gcacctgttc ctcctaagtt
    85741 gacaaagctg tcaaagaaag gtttgttcac gataaacggt gcgctatgcc cagctgcgat
    85801 tgcattaacg tttgcttcaa tggctggcgc attgattgtt tgcatcaaag gatcgaccat
    85861 attcgctccg tgtaaaccaa agaaccataa gaatggtgtg ataaatgcaa gcagtaaggc
    85921 agatggataa gagtttgcaa gtcccataaa tggttcttgg accactgtat agaaagaacc
    85981 tacgatatca tcaacaccga atgcagcaaa aattgcagca actaaagcaa acgcagagat
    86041 agtgatcatt gctggtagta atgaagcaaa tgcttttgca actgccggtg gcacaccatc
    86101 aggcattttg atgatcaatt tttcatttcc gcttaaacgt acaaagattt ctgttgaaat
    86161 aatcccaaca ataatagcaa taaacaaacc gtttgagctc atacctaatg ctccgcctaa
    86221 agcgaagaaa gagcctaaag aaatcacacc tgcggaaaca ccgtcttttc ggtagccgtt
    86281 agctaaatga taagcaagca agaaagcaat caagacagaa agaatggcaa atgtaccgtt
    86341 ccaagcagca gccccgaatc ctttccatga ttcaccacca aaaattgagt tcatcaattc
    86401 ttggaacccg ggaatcggta agttattgat cattactgct aatgctccta agatcattaa
    86461 cggcatactc accataaatg agtcccgaat cgcaactaaa tgccgttgtg caccaatttt
    86521 tgatgctaca ggtataaaat gtttctccat aaatgcaaca aatttatcca taaatcttcc
    86581 ctcttttctt tactgtagat tacgcgttca atcgacgata cagatcaatg atctcttttg
    86641 ctaaatctgt aaaagctata gatgtcatca gatgatcttg actgtggacc gtcaaaagcg
    86701 tgacttcaac gtgatttcct tgtgcttctt gtgtcagcat ctctgtttga gagtgatgtg
    86761 cttggactaa agattcctct gcatctttga ttttttcatc tgctaaagtg aaatcacctt
    86821 ttttcgcagc agcaatcgct tccattgcat cgctttttgc atttccgcca aacatgatca
    86881 gacccatgat ggcttctaga tttttttgat cttccattta ttgtatcctc cgataatttt
    86941 tattatttgt ttattagttt ttctgcttgg tcgagtactt tttcaccatt catcatgcca
    87001 tagtcagcca tattgattac atctaaagga atgcctttag gtgctagctt ttcagcaaat
    87061 tgattttgca taaagcgtac ttgcggtcct aaaagtaaaa cgtctacatt tttagattct
    87121 agttgattat ccgcatctga agcagataca gcaaagatgt ctgcttccat tccttttgct
    87181 tctgctgcct tttgcatttt tgtgactagg agacttgtac tcattccagc tgaacataca
    87241 agcatgattg ttttcttttc cattttaaac acgttccttt ctatacttac acttgtatat
    87301 aagcaagtag cgtgccaact ttaaaagcgc tttaaaaagt tgatatcatc acatttaagg
    87361 tctataccaa gttgcataca cactaaaaaa gaaagcgcac actaaacaca caaattaatt
    87421 tttattacct tttttctttt aacgtgcaca aaaaaagttg tgacacaaaa cttgtcacaa
    87481 cttttttcaa ataaacggta ttcaaaagct tgcatctgcg gtaataagtc caaccaagtt
    87541 aaaaacataa gatgttgttt ttactttgct attcttatta cttgatgcag gacagctttt
    87601 tcacaacctc tcaataaacg gtgttcaatc agctgaccct gggtattagc tcaaaacgct
    87661 ggattcacaa cctcttttat ttcaaataac cagtaaatac aatgaacatc tacaaaaaac
    87721 ttctatttat ttatagattt cgtcaggcaa atccaattct ctcttatcaa tcaaagtctg
    87781 ataccacgaa gcagattttt tcaatgttct gcttcgttca tcgttcaatt cgattcgaac
    87841 taatccatat cgattcttaa atgcattcat tggtgacaca cagtcagtga atgcccataa
    87901 catatagcct tcgcaatttg cgccttcttc tgttgctttg atcaagtaat gcaaatgctg
    87961 tccgataaat tcgatacggt aatcatcttg cacgattcct ttctcatcca tatactgttc
    88021 ttcatattct cttcccatac cgttttctgt tatcagccaa gggatattat gataattttc
    88081 ttttagatac atggctatat catacatgat tttcggatag atttcccatc ccctggaatt
    88141 attcattttc ttcccaggta aatcaaagac ttcataatat cgttcaggat ggaaaggtgt
    88201 ctcttcattc cattgataac gaggggcgcg aacgcgttta ggataatatt gattgattcc
    88261 aaggaaatct actgtatgat gctgaatcgt ttctaaatcc tctgttgcag gatcgaaata
    88321 aatcgattgt ttttgacaca actccaggaa ctcttctgtg tagtatcctt ttaccatcgg
    88381 atcaaaaaat actcgattat aaaacaaatc atacatataa gcagcttttt ggtcttctgg
    88441 agaattggat cgagcataga ccatttcagg atttaaaata cagccaatcc ttccaccata
    88501 atttccgctg cggtaaatct ctactgcttt agccgttgct aaaactttat gataattcca
    88561 catcatccac ttttttgtgt tttgttcatg cggccagcga acagcatcca aatagcaacg
    88621 cgtttgggga acgattggtt cgttgaatgt aaaccagttt tttaccctat caccgtaacg
    88681 atcaaaggca atttgagcat acttagcata aaaatcaact acttttcttg aagaccagcc
    88741 atcatatttt tccaaaagaa aagctgggac ttcataatgt tccaaacaaa tcatcggttc
    88801 tacgtctgct tcgatcaaag agtcgatcat ttcacttata tgctgagcat attcttcatc
    88861 tactatacag ttctcataat cagtaaaaaa acgagcccaa ttgatagaag tccgataatg
    88921 tgtcagatgg atggatttca tcagatccac atcctgctgg tattgttcca taaaattagt
    88981 tgctacggct gggccatacc cattatgcca aacaaaatgt tcatttttat accagctatc
    89041 tataaacgaa tcctgtcctt ctttttttcc tttccaacct tccgtttgcc aagcagaaga
    89101 tgcagcacca agaataaaat tttttggaat agtaattttc atgatttcct cctgtttttt
    89161 agatcccatc attttccaca cgatttgcta cttttacaaa tggcagatag atcaaaatag
    89221 aaacaataat acaaacaagt tgcgtaataa ctgcaccgat atttcctgct gttgccagat
    89281 aagcattgac gatcggcggc attggccaag ggaccattac aactgctttc gccgcaaatc
    89341 ctaaagcggt tgcaaaataa ccaatcgttc cagtgataag aggcgttaag ataaacggaa
    89401 tagctaaaat tggattcagc atgatcggca taccaaaaat aactggttca ttgatgttga
    89461 agatcccagg agcaagggat aacttggtga tttgtttcat atcttcccgt tttccaacta
    89521 acaatactgc aatcagtaaa ccgatcgtga ctccagaccc gcccatactc atatacatat
    89581 cccaaaatgg catagtaata atgttcggta cttcttttcc agcttcaaaa gcttccgtat
    89641 ttacagcaat tgccgctaaa agcaacggtt cccgtatcgg cttcaccatt tgatttccat
    89701 ggataccaat cacccaaaag acttgcgcaa taaacatcaa tagcaaaata cccggtaacc
    89761 cttgtacgac accttctaat gggcgttgta cgacattgta aatgatatca taaaggtaca
    89821 ttcctgtaag ttctttgatg ataaatcccg cagccgcgat acccgataca gtcagaatcg
    89881 tcgggaataa agcagaaaaa ctagcagaaa cattcgttgg cacggtatca ggcattctca
    89941 gctgtagttt cttctgtttc ctcaaccagc aataaagttc aatagaaagg atggagacca
    90001 acattcccaa aaataaccct ttcgtatctg tatattgttt agccaatacg ttttctacta
    90061 taacagtctg attttcattc actaattcat aaacagttgg attaacagat aaataagaca
    90121 tcactgcgat gataccaggg aatacaccat ccactttatt cagcttggct aattcgatcc
    90181 ctatcagaaa aacagcatag atggtcaaga aacttagtgt gacatagctg atggcagatg
    90241 cgataggctt taattcttct aaaaaacgca agaaacttat ctgtgctaag ccattttccg
    90301 agtcaaagac cattgctgaa aacaatgtgc caaaagcacc cgtaataatt accggaatca
    90361 atgcagcaaa tgcgtttttt atcaccatga tataccttaa attattgatt ctcgttgcca
    90421 cgttccctaa aacattggct aatttatcca taaaactcat tccagatccc ctcattcctg
    90481 cttcttctcc ttgacgtcat ctctttttgt ctattagtca gcgcttacat ttcgatgaac
    90541 agaaactatt tactcctctt ttttaccaaa tataaaacaa aaattcatct gatgtattta
    90601 tcataaaata catcagatga actgtcaatt agtttctttt tctcctatta ttttctgtaa
    90661 ttctctccga tttattgcca tatggacagt cattgcatca aacgcagcaa gtgcattccg
    90721 cctttcgatg gcctgaacga tctctgtatg aatatcgatc gtctcgattt tcagatgctg
    90781 gatatcataa tttttattaa acaaagcaat tgattcatta atgattggga taatatgcag
    90841 caaggctacg tttccgctag cttctgcaat catcgtatga aaatcgatgt ctaattgaac
    90901 atgttcttgt gtgccttctc gaaaagaagt ttcaatcttt tgcgttaatt ttttcatttg
    90961 gacgatttga ttcttcgttg catattccgc tgccaaacct gccatcttag gctccaataa
    91021 atatctcagt tcaaataagt ctcgtatcat ccgatcctga tcttcaatca aagaaaaacc
    91081 gagtgggtca ttggcaatcc ctcgcttatc actgacaaat gtccccgctc cttgccgaac
    91141 ttcaaggata ttcctggaag ctagagcccg cacagcttct cgcaccgtgc ttcgccccac
    91201 ctctaaataa tcggacaatt catattcatt cggcagttta tctcctactt gcaactgcat
    91261 ttcctttaaa tatctaagga tcttgtcagt agttacttct actagggttt tattttcttc
    91321 caaaccacat acctcctttt catctgatgt attttttcgt aattattata acacgtttga
    91381 atcatctgat gattttttgt atacaaaatt gattaatccg ttcatagaca tacaaacgaa
    91441 gaagatattt accataaaaa agactaggat aaaattcact cctagccttc gatcattttt
    91501 attttgaacg acaaaaaata tgatgttcaa taacagacga tcttcattcg attcttgatg
    91561 tcatcttttg ttttttttaa tatatggata aaaagtttga ttacatcgtt tctattctag
    91621 aatttggtcg attctcaatt cttcttctgg tgagaaatct aaattatcca tcattcgaac
    91681 actttcttgt aactgactta accgacttgc tccgaccagt acacttgtaa cagatttttg
    91741 tcgcaaattc catgcaaggg ccatttgcgc taatgattgt cctcgtgata cggcgattgt
    91801 ttgcagtgct ttgatttttt ccagagttga tccaacttgt tcttctgaaa ggaatggtat
    91861 ttccttacga tgtgctctag aatcttcagg aataccatgt agataacgat ttgtcagcaa
    91921 cccttgagca agcggactga acgtgattgc ccctagccct tcttcttcta atactggaga
    91981 tagatcgtct tctagtagtg cacgagaaaa catattgtag cgcatctgat gaataatgaa
    92041 cggtgcttcc tttcttttta aaatttcagt catttttttg tatcttctcc attatagttg
    92101 gatataccaa tatataatgc ttttccttgt cttacaagtt gcatcaacgc ttctgctgtt
    92161 tcttccaaag gtgtatcagg gtctgggcga tgatgataga agatgtcaac ataatctagc
    92221 cccattcttt ggaaactttg gtcacaactt gatatgatgg atttacgaga gccccattcc
    92281 ccatatggcc cttcccacat atgataacct gctttgctag aaataatcag ttcatcacgg
    92341 taggcttgaa aatcttctct aaaaatacgc ccaaagtttt tctctgcagc tccagcaggg
    92401 cctccataat tattcgcaag gtcgaaatgg gtaattccca tgtcgaatgc gcctcttaaa
    92461 atctctcttt gattatggat aggatcataa tcaccaaaat tttgccataa tccgagagga
    92521 gagataaaat cggtaatttt aaacctgaat gaccggattt acgatattcc attttttcat
    92581 aacgtgcact atctgcaata tacaattttt ctcctcctca tcaaatcttt ttaaaacgct
    92641 ttcttgtttt ctatttttag tttactacat tttaaagaaa aggagttaat caaaatgatt
    92701 ttttcgtata tttttttgcc atttgttttt tactcgttga tagatcagaa attgattacg
    92761 tgcttgctgg atttttttat agcgtttcgg ttgttttttg taaaattgct gatgatagtt
    92821 ttctgctggc caaaacgtac cggctggttc gatttttgtg acgattggtt ggtggaaagt
    92881 tcctgaactg tctagtttct gttttgattg ttccgctaat tctttttgtc gttcgtcttg
    92941 ataaaaaatc actggacgat attgcttccc tctatcttga aattgtccaa atgtatctgt
    93001 gggatcagta atttgccaat agattgtcag caactcgcta taacttattt ctcttgtgtc
    93061 ataaatgatc tccacagctt ccacatgacc tgtgtaacca ccacttactt gatcataatt
    93121 cggtttttca acatgtccgc ccgtgtatcc tgacaaaaca gataggatcc cttttttcga
    93181 ttcaaacggc tcgaccatac accagaaaca tccccctgca aatactgcac gttcttgata
    93241 gtcagcatct tgcatggcat gcttggaaaa gtcatatttt tgctccttat caaatccttc
    93301 cgtaatcaat aaataaaagt ccattacatc aggcgtcaga ttcattcgga tagctaatgg
    93361 acgtagtgtt acttctagtt ttgataactg ttctttaacg gatatatttt ctcttgtgtc
    93421 attctttgtc tgaatcaata cttttcgttc ccaatctcgt gtattcggat tcaaaatcag
    93481 attataaagt gtttgaatga atatttgttc ctcatccata gattctcctc ctttccgaag
    93541 aagacaagct agtgacttta gctgtaattt atcataaagt gaggaaataa tcgattgaat
    93601 cgtatttatt caaagaagat aagcagcttc agatgatata agatgatctt tatcttaaaa
    93661 agcatactct atggtttggt ttaactaaaa aacgacctct gaaaaaaggg atgttctcca
    93721 attctcagaa gtcgagtaaa gatgattctt ttttatgagc gataataaac ttcatccaaa
    93781 aacagacctt gtgcagggac ggtttctcct gcatgttgcc gcactttgct ttcaaatatt
    93841 tcatcgattg tttctatcgg catggcacct gtgccgattt ctagtaatgt ccccatgata
    93901 atccgcacca ttttatataa gaatccgtct ccgacaaaag taaaatgaag catttctccc
    93961 tttcgttcga tagataattc atcaatcgta cgcactgtgg atttttttga ttttttcaat
    94021 gcagaaaaac caatgaaatc atgtgtaccc actaattttt cgcaagctgc attcagcttg
    94081 tccatatcca actcttgagg gaaataaaag ctgtggtttc gctcaaatgc tgaaggaatc
    94141 acgttgttcc aaacataata gctgtatttt ttgcccaccg cattatatct tgaatggaag
    94201 cgctcaggca tttcttcgat tcttttgatg acaatatccc gcggaaggta acgattcata
    94261 aagtcgatca tttctgccag tccaatttct gtgtttgttt taaaatttgc tacttgtcct
    94321 cgcgcatggg tgccagcatc tgttcttcct gatccaataa tttctatctc ttcatttgtc
    94381 atctgatgca atactttttc gatttttcct tgaatggttt tatcgtcatc tccaagtctt
    94441 tgccatcctg aatatctttt tccgtcatat tcgattgtca acttgatgtt tctcatattt
    94501 tagtggctcc tctgtcttcc atttggtctt ctctctacct ttttaagatt gtagcacaaa
    94561 atatagctcc ttgcttctct aagacagaag ctatacaaga aaactgtgga aatatatatt
    94621 tcagaaaaac aaaaaacagc caccggatga agtatccgct gactgttcaa tttgacggct
    94681 caagttttac tgtttgagat gcaagatact caaccaggca atgggcacat aattaaagaa
    94741 aaaactgatt ctttggcaaa aacctatttt attcagtaat ggccaagaat ttatagaagg
    94801 aattcttgcc agtccataaa tcgccgcaaa aagcaaactg atcacagaca gtccaaggta
    94861 taagtggctt acgtccattt gcccattttt tcgatagatc aagaaaagaa gaactggaaa
    94921 caataaaaaa ccactgtaac ctaaacctga tccgatatta tggacccatg tcgagacatt
    94981 ccacgaagat tcatgggtat tgatactgaa caaacctgta aagatacaat cacctatacc
    95041 atagaacgcg atggctgctg taactagggt agctaacaca cgagatgtac ttacaaatgc
    95101 agcatacaat gcaggcaatg acaggatgaa aaagatccct gaaaccacag accaaataag
    95161 aaacgctttt cttacaggac tggaaacctc tccaaataca ctgatcacgc tcttcatttg
    95221 atctaattca ggataataca atcctaatac gtacggagtc aaaaaatcac tcagcacgcc
    95281 taataataaa aagaaaaaac caaatttacg taaaaaatcc atgatacgcc tccaataaca
    95341 attacctatg cttttaattg ttattataaa cgactttatc ttgatttaac agaaacctct
    95401 atttacacga acatctatat aactcattaa aaactcgaat cattttaaaa atactaaata
    95461 tctaacgttc aacgttcact taatccatct aaacaatctt tcaatttgat tttttcccca
    95521 cttcttttac gcgcgcctag ctctcctatt gcagtaaaca acagatcact tgacgaattt
    95581 aaggcagttt ccattgaatc ctggataaca ccgataataa atccaatacc aacgatttgc
    95641 atcgcgacat cattagagat accaaacaaa ctacatgcta atggaatcaa taacagtgaa
    95701 ccgcccgcaa cacctgaagc tccacaagca gctaaagcag acaaaataca taaaaggact
    95761 ttcaaaacaa atggtgcttc aatccctaaa gaatgtacag cagtcaacgt catcaaggaa
    95821 atggtaatcg cggcaccgcc catattgatc gtagcaccta acgggatcga tatggaataa
    95881 gactcttttg tcaatttcaa tctttcagcc aacatcatgt ttatcgggat atttgcggca
    95941 gaacttcggg taaagaaagc gggaagtgcg ctttctttca aacaaaagaa aatcaatgga
    96001 tagggatttt ctttaataaa caaaaacgtg aggaatgggt acacaactaa tgcgacaaac
    96061 agcatcgtac cgatcaaaac aagtaaaagc tgcagatact ctgccaatcc agcgattcca
    96121 gtcgtagcaa ttgattgata aaccaatcct aaaatcccta tcggagcaaa tgcaataatc
    96181 acttgtacga cttgtgacaa cgctatagat atctgatcca ccaactcttt tgtcggttct
    96241 gaacgtgctc gaaaagctag tcccaacaat acagcccaga ataaaacgcc aaggtaattc
    96301 gcatcaataa gagattgaac aggattttgt gtgacattcg tcaacatagt agataaaaca
    96361 ctttccaaac tttgcggtgc tttttcagat actgtttctt gtaaagtaat aggaacagta
    96421 aaaattgaac ttacaatgac tgctacaagt gcagaaagca atgttcccac taaatataag
    96481 atcaaaatag gcttcacgaa tgtcttattc ccaattttat gcttggcaat tgacgccatc
    96541 gtcaagaaaa aaaccagaat aggtgcgatt gccttcaagc aactgacaaa caacgtcccc
    96601 aatattgaaa taaatgtcca cgtggggaaa aagataccaa acaatattcc gactaaaata
    96661 ccaacaacaa tacgttgaac taacgacata tccaaaaact tctctaacaa atgaccaccc
    96721 tttcacaatt ttaacaatca ttgtatcatt ttatctaaag aaactaaagg tgcaattttc
    96781 taaaaattct aataatacaa ttggcaattc atgctcttgc aacattcaat tttagtattg
    96841 gagggtttat tcaatctcta aagaagtcga gagaaaacta tttatcgtta ttattatagt
    96901 tatccaagta aaacgtaact aagatgaagg acgaaactag aaatttcatc cttcatctta
    96961 attcttagac atagcaatca attaatattt tccactgagt ctcttctttc tctcactctc
    97021 actggtttac ttacccataa acgatttgat taaagccatt tttcaattca ttattcgctg
    97081 tctcgttaaa gtaaagataa ttatcgggat tcccctgatt ttgttgtgtc accaatactt
    97141 ttggctgatt ttgctgaaac ccgaatagat agacgtgctt tttcagatat ggttctgtct
    97201 ctgcatcgga atagacggct gtgatttgat agtctgcttg acctgtccca ctttctgacc
    97261 attctgcaga aacaggcact ccaccaatag ccatcttcca ctccccgttc aaaataactt
    97321 gagggacttt aagaccatat aagtccacac tcatttgatt cgtatagctt ttgtattgtt
    97381 gtcccattgt ttggccccat gtagccataa atgcctctaa ttgtgcggat ttgtctgcat
    97441 tccacagtgt ttgttcgact tgttcgctgc tttctgaaga tgaagcctct acagaagaac
    97501 ttgaagtaga tgtttgagtt gtttcagtcg ttttggaaga ctctgacgaa gtagaggtat
    97561 ctgctgtttc cgtacttgct gcagttggct tgctctctgt gctagttgtt gtagtagttg
    97621 caattgttgc tttcgctgtt tcttttgttg attctttttt cgattcattc ccgttggtac
    97681 aaccagctaa caaaaaagct agaccaattg ttactaaaat ccttttttta aattaaaaaa
    97741 ctcctttcga ttactcccac ttctttattc tatcaaataa gcaatgtgat tttattgatt
    97801 ttttgtattt tctcatccat cttttaaacc ttttaaaaac taagaagcac ccgctgatgt
    97861 tgtctgtaat acatcagcgg gtgcttcttt tattttaatc ttcgcgattt tccaatttac
    97921 ctcacgcttt atatttgtat ttgaagaaac atagactttt agtcatcatc gtcttcctca
    97981 tccaaatctt gtcggacagt ttttccttgg agtctttgta cttggtagat gatttcttta
    98041 ttgaaactta aaagttcaac aagcaattgt ttcaagccat atttttcttc tttttcagct
    98101 aatttgattg cccgtgtgat aaagacattt tggaaatcaa aatcttttgc aaagttttcc
    98161 aacattgttg aggctggtaa atatttgttt tgaccatgtt cgtaaatttt tccataacgc
    98221 atgacttctt ctaaagtaga cggaacgatt tcattttcaa tcactaactc atgagagatt
    98281 ttattgaacc atctcgcttc ttgatcagca tattctgaaa aaagggtact taattcttgc
    98341 gaagatgtac ccaaaacata caattgtgct tggatcaatt tcatttcttc taatttaaaa
    98401 tttgccaata catgccccac catggcgcca gcagttggtt tatgatgatc gatatctgct
    98461 tgttttgttt cttgagcaaa tttttcttcc actatattca ctgatttacg cctcttttac
    98521 ttttgttgat tggacttcat aacctaattt ttccacaact gcagctaatt gttcagggtg
    98581 gacttcatct ggatcaaagt ttgctttgat tttgctcgca ttaaacaaaa ctttcgtatt
    98641 gcttacccca ggctgttgag aaaccgcatg ttctattttt tgcatacagc ttggacaagc
    98701 tagtgtatct aattgcataa tcattttttt catcgtagtt cttccttcct ttttcttgat
    98761 agtcttattc tagattacag cttccataaa aaaattgatt cttatcaagt tttacgattt
    98821 ttcttctttg ctttcgtgtc caaaatacaa taaacgcatc gcgttaaaga taacgatcaa
    98881 gatactagct tcgtgaacga acatcccact agccatgtaa ataaacccgg caaataagcc
    98941 gatgagtaaa aataagacga caccaatggc aatcactaca ttttcacggg tattcaaaac
    99001 agttttctta gccaaacggt aagcatgaac caacgatcca aaactagatt gcatcaagac
    99061 aacgtcactc gtttcgatag cgacatccgt tcctgatccc atagcaatcc cgatatcagc
    99121 cgcagccaaa gagggagaat cattgattcc atctccaatg aaagcaacgt gcaatccttg
    99181 ttcttggtat tttttcacaa attgaacctt ttcatctggc aataattcag catggacttc
    99241 atcaatgcct aacatgtcag ctacataatc agcagtcatc tgattatcac ctgtcagcat
    99301 cactaaatgt tttgctcctg cttgtttcaa catagctaat tgttcagcga cttctggtcg
    99361 aatcacatct gacacaccaa tgatttgagt gatttttttg tcgattgcta caataatgac
    99421 tgagctgcct ctttgttgta attgatgtaa atcttgtact tgctttggtg tgagagaaag
    99481 tttgtgagcg gcaatcaatt tggtgttgcc cgcacaaact gtatgaccat caatctcagc
    99541 tgtgattcct tgtcccttga tcgtctgatt atctttgact gctaattgtt gaaaatcaat
    99601 gtctttctcc tgagcataat ggatcacggc gcgccccaga ggatgatcgg ataacgtttc
    99661 taatcgagcc actaaagcta agctgtcccc ttcatcctct gtatagttct tgatagtgga
    99721 tacagcagta gttcctttgg tcaatgtacc ggttttatca aagacaaaag tatcgatttt
    99781 ggaaaaagta tccattgctt caccgccttt gactaagatg ccccgtttcg ctccattacc
    99841 gattcctgct acattcgaaa caggcgcacc aatgactaaa gcgccaggac aaccaagaac
    99901 caaaacagtg atggctaaac gaaaatcacg actgatcaga ccaacaagca acgcaataag
    99961 taaaacggtt ggagtataat actgagaaaa ttgatcaatg aatttttccg catgtgattt
   100021 ggtatcttgt gcttcttcga ctaattctat aattttggca aaggtcgtat catctccgac
   100081 cttagttgct tcgatcgtca cgtagccatc tgctaaaatc gttccagaaa atacgttatc
   100141 tccactgatt ttgacgactt gtcgtgattc acccgtcact gcggcttcat ccgcataccc
   100201 tttcccgtca atgactcgtc cgtccacagg aatagctcca ccagttttta ctaagacgat
   100261 atcgccttct tccacgtcat cgatttccac ttcttcggtc gttccatctt cttggacaac
   100321 gctggcagtc gatggggcca tctcggtcaa agccttgatt gattcccgcg ttttggtcaa
   100381 tgttttttgt tccagataac taccaaacaa aaacaagaat gtaacgatag ctgactcctt
   100441 gtattctccg atcataaagg caccaagcac agcaatagta acgagtaaat caattgagat
   100501 aactttgact tttactgcct gatacgcatg gattataata ggtatgaccg caataataga
   100561 agctatggct agagcaatgt tatagaacaa gacaaatcca gtaaatttcc cgataaaacc
   100621 aaaaataatc aacacagctg ataaggcggt gatatgattt cgatgctctt ggatccactt
   100681 ttgtaatttc atctttctcc ctcttttcct tttcattctt acgagactaa gtatagatac
   100741 ttatccatca taaaaaattg accactatca agtttattca aaaaggcggt tttcttttgg
   100801 tatactgaat tcctacagtc attaaacgat catatagaaa aaggaagcgc cgaaacgctt
   100861 ccttaggtag ttctattttt ttattcaaag ataagatgct ttgtcatctt acgactgaag
   100921 aacaacaatc aaaaaagggc agcagccact cattcacaag aaatatcgtt ttttgatatg
   100981 ggagttgaag cagtggacaa atatctctac ttattagctg ggaataagat ccaaaaaagc
   101041 ttaatggatt ttatccaaga attggaatgt actttccaca aaaaattcac tcattccatt
   101101 cttttaaaac ttttgattca tacagcgtgt ctaattgagc gtacattgat aaacggtcat
   101161 gaacttaaaa ttatatcaga agatgacaca agaccttctc atgaaacaat ttttcatgta
   101221 aaaaaggcct tcaaaaatat tgaaacagaa tttgggatca ctgtttctta tgatgaatgt
   101281 ttctttatct atgatattat cgcatctaaa taatcttttt ttatgtaaac gagtat
//
LOCUS       DACGLW010000004        74353 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.004, whole genome shotgun sequence.
ACCESSION   DACGLW010000004 DACGLW010000000
VERSION     DACGLW010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 74353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 74353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..74353
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.004"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            complement(179..586)
                     /locus_tag="IXT53_000345"
     CDS             complement(179..586)
                     /locus_tag="IXT53_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAQ4904205.1"
                     /translation="MKFLRKAGVKIGDVIIIGILIIPSFIPLFVFSIEKENETTEDAI
                     KYAIVQIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVR
                     TGWISKPGQTSICLPHKLVISIEKKESKDYYIY"
     gene            complement(775..1602)
                     /locus_tag="IXT53_000346"
     CDS             complement(775..1602)
                     /locus_tag="IXT53_000346"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HAQ4904206.1"
                     /translation="MPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGISKPIPII
                     QAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVVEEEQDI
                     RGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVDLSKILN
                     TNLFNIDETSYSMGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFAHLTDKN
                     FPGNITRAKGFLWFAQHSDFSTIFETSGSHKSIESLNYWVASLPKIECQMIIQKNPN"
     gene            1911..2129
                     /locus_tag="IXT53_000347"
     CDS             1911..2129
                     /locus_tag="IXT53_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ4904207.1"
                     /translation="MSQYCHLTILKRERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTNHLPKRKKIMLIEEKNADVITF"
     gene            2150..2785
                     /locus_tag="IXT53_000348"
     CDS             2150..2785
                     /locus_tag="IXT53_000348"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ4904208.1"
                     /translation="MVRHLFVDFQWSPEKISNRLKHEHMDFSISFITIYRAICNDLFD
                     TEPLSHGNKGLIRSLHHRGKTRHTKNHVESRGKICLTHSIHGHPKSADDRSDFGHWKA
                     DIVAGKTSGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEVLNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(2978..3217)
                     /locus_tag="IXT53_000349"
     CDS             complement(2978..3217)
                     /locus_tag="IXT53_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4904209.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYQFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(3241..4764)
                     /locus_tag="IXT53_000350"
     CDS             complement(3241..4764)
                     /locus_tag="IXT53_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAQ4904210.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLIQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKLNKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNEMHNWPTFYPASLSEHEIAQEMMAH"
     gene            complement(4782..4904)
                     /locus_tag="IXT53_000351"
     CDS             complement(4782..4904)
                     /locus_tag="IXT53_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HAQ4904211.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKQNKMKKLA"
     gene            complement(4934..5917)
                     /locus_tag="IXT53_000352"
     CDS             complement(4934..5917)
                     /locus_tag="IXT53_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HAQ4904212.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVIADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLEKFNHPLVFESDSSENH
                     YNGKHYHQHHQEHEFKSILLTSDQDIKKDFLFQLIDRLILTNQGSLYRFKGLIALQDQ
                     DFIVAIQGVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(5941..6090)
                     /gene="rpmG"
                     /locus_tag="IXT53_000353"
     CDS             complement(5941..6090)
                     /gene="rpmG"
                     /locus_tag="IXT53_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ4904213.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(6111..6488)
                     /locus_tag="IXT53_000354"
     CDS             complement(6111..6488)
                     /locus_tag="IXT53_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4904214.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(6520..6699)
                     /gene="rpmF"
                     /locus_tag="IXT53_000355"
     CDS             complement(6520..6699)
                     /gene="rpmF"
                     /locus_tag="IXT53_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAQ4904215.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(6714..6983)
                     /gene="rpsN"
                     /locus_tag="IXT53_000356"
     CDS             complement(6714..6983)
                     /gene="rpsN"
                     /locus_tag="IXT53_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAQ4904216.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            complement(7506..9278)
                     /locus_tag="IXT53_000357"
     CDS             complement(7506..9278)
                     /locus_tag="IXT53_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002841847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4904217.1"
                     /translation="MRMQMIGGLTMKSFYKNKFALTNQGAEDLVKASISSFFVYCINM
                     VPAFIIMMLIDELVLENVKPPWLYFTVSFVTLLFMYWLLDREYENLYNSTYKESAHLR
                     MQIADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVM
                     LLVGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEI
                     NSFNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLL
                     TGEVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSP
                     LKSFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDY
                     DEGCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAA
                     RLANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLD
                     AENEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKIVDQGTHSELYRRSE
                     IYKNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(9232..10986)
                     /locus_tag="IXT53_000358"
     CDS             complement(9232..10986)
                     /locus_tag="IXT53_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4904218.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLK
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLMKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(11096..11665)
                     /locus_tag="IXT53_000359"
     CDS             complement(11096..11665)
                     /locus_tag="IXT53_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HAQ4904219.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(11683..13107)
                     /locus_tag="IXT53_000360"
     CDS             complement(11683..13107)
                     /locus_tag="IXT53_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ4904220.1"
                     /translation="MKELFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSG
                     KSTLLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTN
                     TTSELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLS
                     LNSKLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVK
                     NRTVKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDIN
                     FDVKVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQF
                     FTESVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSK
                     TNLFLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGG
                     IKKDIKLRVEDSTLIEQIFNDITK"
     gene            complement(13109..13777)
                     /locus_tag="IXT53_000361"
     CDS             complement(13109..13777)
                     /locus_tag="IXT53_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HAQ4904221.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFGTSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(13908..14492)
                     /locus_tag="IXT53_000362"
     CDS             complement(13908..14492)
                     /locus_tag="IXT53_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ4904222.1"
                     /translation="MESGLSHFEKVGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLV
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(14827..15057)
                     /locus_tag="IXT53_000363"
     CDS             complement(14827..15057)
                     /locus_tag="IXT53_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HAQ4904223.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLTKLRYEDYLDIGFQLTDKGKKFIDDYKKRF"
     gene            complement(15072..16022)
                     /locus_tag="IXT53_000364"
     CDS             complement(15072..16022)
                     /locus_tag="IXT53_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAQ4904224.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSTNSKDKDTVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(16019..16861)
                     /locus_tag="IXT53_000365"
     CDS             complement(16019..16861)
                     /locus_tag="IXT53_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ4904225.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVTVVLFFKELLIT
                     SFDPLMAKAMGMNVSFYHYLLMILLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGACSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERKL"
     gene            complement(16994..17719)
                     /locus_tag="IXT53_000366"
     CDS             complement(16994..17719)
                     /locus_tag="IXT53_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ4904226.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKELKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGGTA"
     gene            complement(18180..18494)
                     /locus_tag="IXT53_000367"
     CDS             complement(18180..18494)
                     /locus_tag="IXT53_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAQ4904227.1"
                     /translation="MHKDLLIICETGISASLLVSKMLETIRAKNLSYEIDYATIRNVE
                     EKLNYKDYDVLLLTPQVARSEEKVKEIIKKEKCTAQIVYIKQEDFRYMNIENIIASIE
                     ED"
     gene            complement(18510..20105)
                     /locus_tag="IXT53_000368"
     CDS             complement(18510..20105)
                     /locus_tag="IXT53_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HAQ4904228.1"
                     /translation="MNLFSIISFLVIVICVWLFAYSRSRSVDTSGSEGFFMGGRSLTA
                     LPIAGTIIMTNLSTEQIVGQNGQSYHAGMEVMAWEVTATIAIVALAVIFLPKYFKYGI
                     NTISDFIEIRYDTVTKRIISILFIVTYMTSFLPVVLYSGSLVFNKIFHIDELLGVQPI
                     VAIIIVAMAIGIVGILYLLIGGLSLSANSDSIYGVGLLACGLLVPILGLMKLGDGSFV
                     GGIEHVVVNTPWLLNSVGSIDSAVVPWPTLFTGMLFNNLYFWCTNQMIVQKALSGKNL
                     AEAQKGAFLVGVFKILGALFLVFPGIVARNIFGDSLMSNPDNAYPFLVSEVLPKMIFG
                     IFAAVIFGAILSSFAGALNATSTLFSLDFYKPLINKKANDKQIARAGKTVTVVVGIIS
                     IIVAPFISFAPAGLYQFVQEFNGLYNMPLLVIILFAFYSKKATAFGAKTTIAVHIVLY
                     ALSKMLFQNIHFLYVLTALFVLDIIILMVVSKLKPDGEFVLNSFDTKVDIEPWKYTKV
                     TAIALVVIVILTYVAFSPIGFAG"
     gene            complement(20128..21018)
                     /gene="iolE"
                     /locus_tag="IXT53_000369"
     CDS             complement(20128..21018)
                     /gene="iolE"
                     /locus_tag="IXT53_000369"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015076872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HAQ4904229.1"
                     /translation="MTKQIQLAIAPIAWTNDDMPELGSENTFEQCISEMALAGFTGTE
                     IGNKYPKDPEVLEQYLNIRGLKVASAWFSAFLTTKPFEETAEAFKAHRDFLHAMGAKV
                     IVVAEQGHSIQGIMTAPVFDDKPVFTDEEWKKLAEGLEKLGDLAHEKDMEIVYHHHMG
                     TGVQTTEEIDRLMEMTDPDKVKLLFDTGHLVFSGENPIAVYNRHQDRIKHIHFKDIRQ
                     ETAEEVEKEKDSFLNGVKKGVFTVPGDGMIDFTPIWTAIHASDYEGWIVVEAEQDPAK
                     ANPFEYAVKARSYIREVTGL"
     gene            complement(21035..22060)
                     /gene="iolG"
                     /locus_tag="IXT53_000370"
     CDS             complement(21035..22060)
                     /gene="iolG"
                     /locus_tag="IXT53_000370"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HAQ4904230.1"
                     /translation="MNNIRVGIVGLGRLGKVHAQNLATGVSCCELTAACSLVETELAF
                     AQKKLGVKHTFSSYEEMVKSSVIDAVFIVSPSGFHCDQVRLAMENGKHVFTEKPLGLE
                     VEDIKKTQKVIEEHPEQVFMLGFMRRYDESYQYAKKMVEDGEIGEITLIRCYGIDPSE
                     GLESFVKFAMNNDSGGLFLDMAIHDIDLVRWFTGQEVDQVWAIGKNAAYPELDQAGEL
                     ETGAAMMSLSDKTIALLVAGRNAAHGYHVETEIMGTKGMLRVAQAPEKNLVTIMNDQG
                     IIRPCSQNFPERFREAFIREAQEFVSCIQEKRQPAVNAEDGLQSTKIALACQESFDTK
                     SLVQLKK"
     gene            complement(22084..23091)
                     /locus_tag="IXT53_000371"
     CDS             complement(22084..23091)
                     /locus_tag="IXT53_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ4904231.1"
                     /translation="MRVKVGVIGTGAIGIEHIDRINQKTQGAYVTAVSDINVKAAKEV
                     AKRVDAKFFETGEALIASEDVDVVVVTSWDPTHEQYVLEAIKHGKYVFCEKPLAMDAA
                     GCRRIVDAEVAYGKKLVQVGFMRRYDRGYIELKEAIESKQYGEALMMHCAHRNPSADE
                     NYTTPMAISNTAIHEIDVLRWLLNEDYESVQMILPKKTSNSHADLHDPQLLIFQTKSG
                     VTIDLEIFVNCRFGYDIKCDVVFETAEVGMTDPAYTRIKSEEKNYTPVSPDWQTRFLD
                     AYDYEFKLWIDSIKNDAVVGPTAWDGYVAAITMMACHESRETKTKVMIQMDERPALYN
                     E"
     gene            complement(23204..24364)
                     /locus_tag="IXT53_000372"
     CDS             complement(23204..24364)
                     /locus_tag="IXT53_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HAQ4904232.1"
                     /translation="MNIQLKMPANLRIGGGTIQFLMEESEQLGNKPLIISDTVMEQTG
                     HLQKIKELLGKTDDQLAEYTDIQAEPTQENVYDALKLFQKHQCDHIIAFGGGSCLDTA
                     KVVAVLAANQLSLEAIHARNFDQLQAGIPLIAVSTTAGTGSEVTDVAVITNTKTAVKM
                     MMKDLVFMPQISIVDSDFTKTVPPKVTAATGVDALCHALESYVSVKANEVTQLFSLEA
                     MKKILNTLSQAYENSDDTEAREQMSLASTEAGIAFSNASVTLIHGMSRPVGALFHIPH
                     GISNAMLLPVFFEFSKDAIQTELATIARFIYPAAEDLSLEEAADYLIKKIQELCEQLS
                     IPTIRDYGIDQQAFMAALDKMAADALTSGSPQNNKKVPSHSQIKELYVQAYG"
     gene            complement(24440..25894)
                     /locus_tag="IXT53_000373"
     CDS             complement(24440..25894)
                     /locus_tag="IXT53_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010825391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HAQ4904233.1"
                     /translation="MTRFLRNYIDGRWVASRSTEFEEVMNPATEEALANVPLSSKAET
                     AEAVKAADAAFQTWKKVPVPVRGRYLFKLHQLLTDHKSELARLITLENGKSLSESLGE
                     VQRGIENVEHAAGITNLMMGDSLSTAATDIEITNYRYPIGVIGGITPFNFPMMVPFWI
                     FPMALAAGNTVVLKPSEKTPLLMEKIVELVEKAGFPSGVFNVVYGAHDVVNTLLQDPL
                     VKGISFVGSKNVGEYVYREGTKNLKRVQALTGAKNHVVVLKDANIDRAVKDLIGGAFG
                     SAGERCMAASVIVLEEEIADQFVEKFKAAAKAIKIGNGLDEGVFLGPVIRKDAQQRTF
                     DYIQKGVDEGAKLVLDGRENIPEKGYFVGPTIFDNVTMDMKIWQDEIFAPVASIVRVK
                     DLMAAVELTNESEFANGSCLYTNNASAIRYFRENVDAGMLGVNLGVPAPMAWFAFSGW
                     KHSFFGDLHANGKDSVEFYTHRKVVTARYAQSEI"
     gene            complement(25958..26782)
                     /gene="iolB"
                     /locus_tag="IXT53_000374"
     CDS             complement(25958..26782)
                     /gene="iolB"
                     /locus_tag="IXT53_000374"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HAQ4904234.1"
                     /translation="MEKTLKHQPLNKELAPGVKQIQNIRQSNSALSYIEFQILELEKG
                     ASYKETGSQQEVCIVILTGKIKVKAADTWYEDLGTRESVFEKIPTDSIYLPAEHSFEL
                     VATTDNVRLALCYSPAKKALPISVIRAEDNTVEERGLYQNKRLVHNILPDNVDTASSL
                     LVVEVYTEGGNFSSYPPHKHDQENLPHESFLEETYYHELDPEQGFVFQRVYTEDRSLD
                     ETMTVEHQNVVEVPKGYHPVGVPDGYTSYYLNVMAGPERIWKFHNEKKHEWILQRK"
     gene            complement(26821..28737)
                     /gene="iolD"
                     /locus_tag="IXT53_000375"
     CDS             complement(26821..28737)
                     /gene="iolD"
                     /locus_tag="IXT53_000375"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002397168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HAQ4904235.1"
                     /translation="MEKTVCLTTAQALLKFLNQQYISIDGKETPYVEGIFHVYGHGNV
                     LGIGQALELEPGHLKSYSGKNEQGMAHAATAFARQNLRKKIFAVSTSVGPGAANLLTA
                     AGTAFANNIPVLFLPADTFATRQPDPVLQQLEHASSVAFSTNDAFQAVSRYWDRINRP
                     EQLMSALLRAFEVMTNPATMGPATICLPQDVEAEAYAYPEEFFKKRVHYIDRRIPTAR
                     ELAEASQRIKASKRPVIIVGGGCKYSEAGEILEQISRDCQIPLVETHAGKSTVSWDFK
                     NNLGGMGILGTSAANKAVQQADLIIGIGTRYTDFTSASKSIFNFEQAKMMNINVSRQQ
                     AAKLEAFQVVGDAKATLEQLAPMIEGYQTAYGEKIDELKEEWISERNRLKAAHFKRKD
                     FTPEIKDHFSQEILNEYADALATNLTQTEVFIHLNDFVDEDAIVIGSAGSLPGDMQRL
                     WNPVKENTYHLEYGYSCMGYEIAGAMGVRMARPEQEVYALVGDGSFLMLHSELVSSLQ
                     YDKKINIVLFDNSGFGCINNLQMDNGGASQGTEFRNTKDEIMNIDYAKVAEGYGAKTY
                     RVSSIAELQAAVEDAKKQTRSTLIEIKVLPKTMTEGYDGSWWNVGISEVSGKAAMQEA
                     YKAKQAKLATIRKY"
     gene            complement(28748..29782)
                     /gene="iolC"
                     /locus_tag="IXT53_000376"
     CDS             complement(28748..29782)
                     /gene="iolC"
                     /locus_tag="IXT53_000376"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018593001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HAQ4904236.1"
                     /translation="MSYIKFDQSKEFDLLLLGRVAIDFNPAYNDQVKEEFKPLKKVHM
                     FEKFVGGSPANIAVGVTKHGMKAGFIGTVSDDQFGEYVIDYFNEQGIDTSQISKAQHG
                     EKLGLTFTEMLSSKESNILMYRNQAADLQLSVEDIDEDYLKRSKALLISGTALAESPS
                     REAVIKAVMLAKRNNVKIIFDIDYRAYNWKNNDDISIYYSMIAKESDIIMGSREEFDL
                     TEKLIQPNMSDNESAAFWHSCGAEIVVIKHGMKGSTAYTADQQQFSIKPFPVEARKGF
                     GGGDGYGSGFLYGLFEGWEIIDCLEFGSAEASMMVKSNNCSDSLPTTQEVKDFITLEK
                     EIYGEMIARI"
     gene            complement(29939..30955)
                     /locus_tag="IXT53_000377"
     CDS             complement(29939..30955)
                     /locus_tag="IXT53_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016633586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ4904237.1"
                     /translation="MAGTIKEIAEKAGVSRGTVDRALNDRGRINPEVAERIRAIAKEM
                     NYVPKKKKVIKETTLKLGIVTQLAKSSFMIPIRSGLESIIGDLKKRNIDCFLEEVDGV
                     DEAAQLQALDRLVELGVKGIAIMPVDSDGIREKINQLTDQGILIITFNSDILGTNRQC
                     FVGMDNFKSGKTAAGLLGMLTKGKGKVLAITGYFGNSVNSLRVAGFVEELKESFPELE
                     LTGVQSSFDSSAEVEKILSNTLENYSNLDGVVVFSGGQAGIARALEKIDESKRPFVII
                     YDLTKNNIEMLKNDQVDFLIDQDGFVQGYRSLLMLANQLQNNKTVTKDALFTDIIIKT
                     KYNI"
     gene            complement(31057..31254)
                     /locus_tag="IXT53_000378"
     CDS             complement(31057..31254)
                     /locus_tag="IXT53_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4904238.1"
                     /translation="MKFICLSCGEKESIPMDVVKFLDDADIANDPNNPPQFTCEKCGK
                     EMYPEYYRNALGIEFKISDVQ"
     gene            complement(31226..32545)
                     /locus_tag="IXT53_000379"
     CDS             complement(31226..32545)
                     /locus_tag="IXT53_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011674482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HAQ4904239.1"
                     /translation="MKANILEAIFFDEHHNWDNFVEKYGRKIRLVVKREVNKFRYCGD
                     IQKGYRLFVCEGCHQTKFVPLKCRGKFCPTCATGESQRWSAVVANDMFKVTHRHVIFT
                     IDEGLREIFMMENYRDSLLKGLMDEAAKIILAYFEKRKVKAGIVATIHTFGSKLEFNP
                     HVHMVVTMGGLTRDGSWEEYDYLPFSMLRKYWKNAVLKLIRRTLGEWDKQRVQSRLQA
                     AYKNNGEGFYVNAPKRSRTNLKSLLQYISRYMKRGPIALSRILMYDGDTVMFNYMDKR
                     TNTKETMTMSIDEFIAALIRHIQNKNFKTIRRYGIYSRRIKTLMKKVMKEYQKRVKKL
                     FINLNEALRPKSWEERITEEFGENPLECSCCGEYYEFVGMSVRKNGRLVIKYAKDNHA
                     KRFMREENRKIDQKEFRQQYEEEKEKTFKAIRWDWERQREIYMSKLW"
     gene            complement(33286..34266)
                     /locus_tag="IXT53_000380"
     CDS             complement(33286..34266)
                     /locus_tag="IXT53_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAQ4904240.1"
                     /translation="MLFLKKEDIIKSFSMREAIDADKKALSLYSAGKASVPLRTNIDV
                     PKSNGQSLYMPAYVEGGEGALGVKIVSVYPENIKKNLPSVPATMIVLDPETGMVSACL
                     DGTYLTQLRTGAVQGAATELLAREDAKIGALIGTGGQAQSQLEAMLTVRKLEEVRIFD
                     IDFERASQFAEKMMQQFSVTMRPTKTSQECVEGADIITSVTTSKRATFSAEWVKKGAH
                     INGVGAYTPEMCEIPREIIKAADVVIFDTMDGVLKEAGDFISPLQDGYIQRDSYHGEL
                     GQLINEELIGRTSGEQITIFKTVGSAVLDVVVATEIVKKAKENNLGKLIF"
     gene            complement(34279..35157)
                     /locus_tag="IXT53_000381"
     CDS             complement(34279..35157)
                     /locus_tag="IXT53_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HAQ4904241.1"
                     /translation="MIRNILLTLIVAINLYFIIYFLRDLFKNKQNFKEEPGDMRLLPF
                     TSFIIFFLSTFGVSDFAIGTVLYPKLKWVSMKKLPGTLNTQCVVPVAVMALSYITAIN
                     VGIKTLAVCIICQIIGAYLGPRFVVKLPEKTIKLFVGIGLVIAALLIFAGQMNWIPSN
                     GTASELYGGKLILAGFLLFVYGALNNIGIGSYALTMVTVYLLGLNPVAAFPIMMGACT
                     FSVPIGSVQFIKFNEYSRKITLFTSTFGVLGVLVAVFLVKSLDTYILKWIVILVLLYS
                     AYTMLGSQLKKVTAAN"
     gene            complement(35173..35973)
                     /locus_tag="IXT53_000382"
     CDS             complement(35173..35973)
                     /locus_tag="IXT53_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HAQ4904242.1"
                     /translation="METIIQIMKKYDFSEMETRVYTTLLEKGNLTGYEVSKISGVPRS
                     KVYNILEKLLKKNLIVVNKSEPKLYHAISANEFLEKLEKSVKNDLSFLTKNLGMIKEK
                     DEEEMLWKVDGIEYVLDKVEHLVKNAKESLLIQVWHENFTDSLLKALQQAEKRVDKFV
                     LILFSSTHEYDLPLEKYYIHGFETDKLADFGARWINIVADEQEVVFGTINEELQSTDV
                     TWTKNHAMVNLAKEYVKHDAYTLKVIAESPEELKAKYGEGFEGIRKIY"
     gene            37365..37691
                     /locus_tag="IXT53_000383"
     CDS             37365..37691
                     /locus_tag="IXT53_000383"
                     /inference="COORDINATES: protein motif:HMM:NF013698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ4904243.1"
                     /translation="MFKIFRKNCCGLDVHKTWIYACIGITDANGRTVYKQARFSSFTK
                     GLNELAEWLAKYNCKDVCLESSGKFWIPVFNILEKTCFVTLAHPKYTKPQKGNKTDRK
                     DAKMDL"
     gene            37699..38607
                     /locus_tag="IXT53_000384"
     CDS             37699..38607
                     /locus_tag="IXT53_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ4904244.1"
                     /translation="MCDMIKPSFIPGLEIRQLRDLMRYRTKLTNMKTSEKNRALNCLT
                     VSNLKLDDVFSDVFGKSSTSIIQYLLEHPGESFDVRPFIHARCKTPVEEIQAAVEGAI
                     SVPQAIKLRQCLNHINEIEKHREEINLEIQALAEPFSPVLELLRTIPGLDTQPITAIA
                     ILSEIGPDMSVFPTSKHFISWAGCCPRNDRSARRTKSTRISRAGQSLKPLLVQIANAL
                     LRSKEHPEFKNRYRRIKANRGHKKAIIAICKMLLTAIWNVLSKVEPYSAKGYLEPKPA
                     KEEKVLTQSQALDLLRKRGYTIIDSA"
     gene            39236..39487
                     /locus_tag="IXT53_000385"
     CDS             39236..39487
                     /locus_tag="IXT53_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HAQ4904245.1"
                     /translation="MKSNQKSGFYTFIKAISSVLECDCIIGPYSMLIKMGFPSTESLD
                     SFINDLSKFGRTNTQIVFSTLVSLMVYQFWNVYHALLLK"
     gene            40029..40187
                     /locus_tag="IXT53_000386"
     CDS             40029..40187
                     /locus_tag="IXT53_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ4904246.1"
                     /translation="MNKKQACEFLGIDNNTLDKWIRVYNFPVIKIDGVVRFDVEDIKD
                     FMNTFKKN"
     gene            40259..41485
                     /locus_tag="IXT53_000387"
     CDS             40259..41485
                     /locus_tag="IXT53_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ4904247.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFREITGIASEKYQKVFPTRQLAIKA
                     EREMKKKIETVLREENANSLELKGKIKFKDFYESKWLPRYELGQTTRSNRAPSNVTIS
                     NTKDIFRLHILPMFGEYAMNYLNSNTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILCYIEFNRTTKIIQCITAPKKMALEEKRIREGNQALSSEELTDWIEAVNNDLNNHLL
                     TLHDYTLFMLTLYLGDRKSETYALQWKYIDFNKNTVRLKYALDKYQRKTHTKGWKDTV
                     IQAPEVVMTLLREWKSAQAEQLLKLKIIQTPDQYLFTYSKPSGEVNCPVHADYLNYRI
                     NAIKKRHPELAQLSPHKLRHTYATIAREGGANMNQISNALTHSDISTTKIYVNTPDVV
                     DKSVFEAFQKGLNKCD"
     gene            complement(41574..41966)
                     /gene="rpsI"
                     /locus_tag="IXT53_000388"
     CDS             complement(41574..41966)
                     /gene="rpsI"
                     /locus_tag="IXT53_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAQ4904248.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(41982..42425)
                     /gene="rplM"
                     /locus_tag="IXT53_000389"
     CDS             complement(41982..42425)
                     /gene="rplM"
                     /locus_tag="IXT53_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HAQ4904249.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(42944..43411)
                     /locus_tag="IXT53_000390"
     CDS             complement(42944..43411)
                     /locus_tag="IXT53_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HAQ4904250.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(43531..45069)
                     /locus_tag="IXT53_000391"
     CDS             complement(43531..45069)
                     /locus_tag="IXT53_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HAQ4904251.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            45149..45835
                     /locus_tag="IXT53_000392"
     CDS             45149..45835
                     /locus_tag="IXT53_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HAQ4904252.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(46036..46563)
                     /gene="msrA"
                     /locus_tag="IXT53_000393"
     CDS             complement(46036..46563)
                     /gene="msrA"
                     /locus_tag="IXT53_000393"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ4904253.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(46647..47324)
                     /gene="pgmB"
                     /locus_tag="IXT53_000394"
     CDS             complement(46647..47324)
                     /gene="pgmB"
                     /locus_tag="IXT53_000394"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAQ4904254.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(47321..48449)
                     /locus_tag="IXT53_000395"
                     /pseudo
     CDS             complement(47321..48449)
                     /locus_tag="IXT53_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
     gene            complement(48449..50632)
                     /locus_tag="IXT53_000396"
     CDS             complement(48449..50632)
                     /locus_tag="IXT53_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAQ4904255.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(50629..51654)
                     /locus_tag="IXT53_000397"
     CDS             complement(50629..51654)
                     /locus_tag="IXT53_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ4904256.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTANRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(51699..52526)
                     /locus_tag="IXT53_000398"
     CDS             complement(51699..52526)
                     /locus_tag="IXT53_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ4904257.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(52523..53590)
                     /locus_tag="IXT53_000399"
     CDS             complement(52523..53590)
                     /locus_tag="IXT53_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HAQ4904258.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(53616..54452)
                     /locus_tag="IXT53_000400"
     CDS             complement(53616..54452)
                     /locus_tag="IXT53_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ4904259.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(54449..55318)
                     /locus_tag="IXT53_000401"
     CDS             complement(54449..55318)
                     /locus_tag="IXT53_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ4904260.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(55388..56671)
                     /locus_tag="IXT53_000402"
     CDS             complement(55388..56671)
                     /locus_tag="IXT53_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ4904261.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(56685..58370)
                     /locus_tag="IXT53_000403"
     CDS             complement(56685..58370)
                     /locus_tag="IXT53_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HAQ4904262.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            58645..59670
                     /locus_tag="IXT53_000404"
     CDS             58645..59670
                     /locus_tag="IXT53_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ4904263.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(60265..63918)
                     /gene="rpoC"
                     /locus_tag="IXT53_000405"
     CDS             complement(60265..63918)
                     /gene="rpoC"
                     /locus_tag="IXT53_000405"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HAQ4904264.1"
                     /translation="MIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYRTLKPERE
                     GLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTEREYREKRE
                     QYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLDILEAFRA
                     SGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLKRLLDLNA
                     PGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGK
                     RVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKNAKRKIER
                     QEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVCEAYNADF
                     DGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTMEEEG
                     REGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMITTVGKII
                     FNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKKKNLGNII
                     AEVFKRFKVKETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIENAHKQVET
                     ITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSGARGNISN
                     FTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTRKEVRHPE
                     TNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNLATGSDVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNPKGQAVIT
                     EVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGAPLTEGSI
                     DPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRVMDPGDTD
                     ILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAASFQETTRV
                     LTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVYSISDIEA
                     QMAAEDAMNNQTK"
     gene            complement(63997..67614)
                     /gene="rpoB"
                     /locus_tag="IXT53_000406"
     CDS             complement(63997..67614)
                     /gene="rpoB"
                     /locus_tag="IXT53_000406"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAQ4904265.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLFALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            68342..69310
                     /locus_tag="IXT53_000407"
     CDS             68342..69310
                     /locus_tag="IXT53_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ4904266.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            69328..70233
                     /locus_tag="IXT53_000408"
     CDS             69328..70233
                     /locus_tag="IXT53_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ4904267.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGSAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            70230..71036
                     /locus_tag="IXT53_000409"
     CDS             70230..71036
                     /locus_tag="IXT53_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ4904268.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            71193..72161
                     /locus_tag="IXT53_000410"
     CDS             71193..72161
                     /locus_tag="IXT53_000410"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HAQ4904269.1"
                     /translation="MTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKAIKELEKA
                     GYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMMTSESTMKDAKLAAAQGD
                     SVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTIIAASACVK
                     AIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLNFSVPQNH
                     FPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKSFVLGKTV
                     SFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(72478..73002)
                     /locus_tag="IXT53_000411"
     CDS             complement(72478..73002)
                     /locus_tag="IXT53_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HAQ4904270.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            73289..>74353
                     /gene="scm"
                     /locus_tag="IXT53_000412"
     CDS             73289..>74353
                     /gene="scm"
                     /locus_tag="IXT53_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAQ4904271.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSS"
BASE COUNT        21745 a        15713 c        12801 g        24094 t
ORIGIN      
        1 aaataagtaa tctaaccgac tgttaactaa accattgcag gtcccgttaa agatacttgt
       61 gcctgttgtc accgcttctt tttcttgtcc acaagcgctt aacacactta ataaagcccc
      121 tgctattatt aataataata tctttttctt attaaaaacc tcctcatcta tttccagatt
      181 agtaaatgta gtaatctttg ctttcttttt tctcaatact aataactaac ttatggggta
      241 aacaaatact cgtttgaccg ggttttgaga tccatccagt tctaaccgcg atttgatcag
      301 gactattatc ttctttaact cgaattcttc catctttaat ctcaacaata ttatattgtc
      361 ccttcgcagg atgataattc acgagtttat gattgatctt atccaaatcg aagcgatcca
      421 tttccttgcc atcaatttga acgatggcat atttgatagc atcttcagtt gtttcattct
      481 ccttttctat agagaataca aataagggaa taaaagaggg aataatcaaa atgccaataa
      541 ttatgacatc gcctatcttg acaccagctt ttcttaaaaa tttcattaac gatatctggc
      601 taaacatatt agaatttttc catggaaatg gtgaaatttt tcggaaatta tctgcattaa
      661 gagaatcagt taaacaaccc tcaagttgtt ccttaataca ttgttgatcc atcttcatac
      721 caataaaaac tagtttatta atgcgttcac caaacaacgg atgccaggct tccttcaatt
      781 cgggtttttt tgtataatca tttgacactc aattttaggt aaactagcta cccaataatt
      841 taatgattca atacttttat ggctacccga tgtttcaaag atcgtcgaaa aatcactatg
      901 ttgggcaaac caaagaaacc cttttgctct tgtaatattg ccaggaaaat ttttatccgt
      961 aagatgggca aatttttgtg cgtcaaggag taaccgattt cgatagataa aacttgagat
     1021 accatattct tctgtttcag gaatatgatt ttgatagcca agctctaact ctttgaacca
     1081 gcccattgaa taggaagtct catctatatt aaaaagattt gtatttagaa ttttcgataa
     1141 gtctacttca cctttaattg tcctaattat tttggcattg gactgtaact tagcaattag
     1201 accgttaatt ttgtaaatcg tcttggttga cactaagtca ctattattta aaagtaatat
     1261 gttacaacac tcgaattgat caattaacaa gccacgaata tcctgttctt cttcaactac
     1321 attaccttca gcttttcttt cctgaatctt ttcacccgtt tcgaaattat cccaaaaacg
     1381 ttttacatcc actactataa taatggcacc aatctctaat cgttgggtca aatcaatgtc
     1441 taaagggttt ttagctaata taattgcttg aataattgga attggtttgc tgattcctga
     1501 agcttcaata acaatttgat tcaagtcaga aaattgcact aattgatcta gttcaacaat
     1561 caaatcttcg cgtaatgtac aacaaatgca tccattcggc atcgaaacaa gtttttctcc
     1621 agttctttta aaacctttct tgtcgactaa taccccatca acatttactt cccctatgtc
     1681 atttacaatg acagcaattt cctttccgtg atcaccgtac agaatatgat ttagaagtat
     1741 cgtttttcct gaaccaagat agccagttaa gattgtaata ggtatttttt catagggaac
     1801 ctctttctat accttgaatt tacactttaa gtgcaacaca taaaaatgag tcataatcga
     1861 tatccaacta gtataagttt gtcgagaaca ctcctagaag gaaaataatt atgagccagt
     1921 attgtcatct tactatactc aaacgagaaa gaatctttct gcttcacgaa gaaggatttt
     1981 ctattagtat aattggcaac gacattcagc atagcccgtt tactatttta catgaactga
     2041 gaagaaacaa gaaaaaaact aatcaccttc caaagcggaa aaaaattatg cttatcgaag
     2101 aaaaaaatgc ggacgttatc acattctaaa tgatccgtga gtttggtcaa ttgttcgtca
     2161 tttatttgtc gattttcagt ggtcgccaga gaaaatctcg aatcgtttga agcatgaaca
     2221 tatggacttt tcaattagtt ttatcacgat ctaccgtgca atttgcaatg atcttttcga
     2281 tacagaaccc ctctcacacg gcaataaagg cctgatccgt tcattgcacc atcgaggaaa
     2341 aacaagacat acaaaaaatc atgtcgagtc tcgtgggaag atttgcctta ctcattcgat
     2401 ccatggccat ccaaaatccg cagatgatag aagtgatttt ggtcattgga aagccgatat
     2461 tgtagctggc aaaactagtg gtacttgttt agtgactttg acagacagaa aatcatgctt
     2521 ccttttagca ggaaagaagg tccccagaaa gaaagccgct tatgtgagcg agaaaatgat
     2581 tgaactttta agcagtattc caaatgaggc ggtcaaaacg atcactccag atagaggaaa
     2641 agaattctcc aatcatttcg cagttacaga agtattgaat aatatgccct tttattttcc
     2701 agatcctcat gcccttggca acaatgaaca aattaaaata cgaatagact cattcgagaa
     2761 tacttgccaa aatcgaaaga gatagctacg gtagataact caacgataaa tcagatgatt
     2821 gaaaaactga ataacagacc tagaaagtgt ttagattgga gaacacctta tgaagtcttc
     2881 tttgataaag tgttgcgctt gatttgacaa ttcaagaaat aataaaaagc agatcaaatc
     2941 caaataaggg aaaaatgatc tactctggta ttatcaatta attggccaag ctattatgcc
     3001 gttttgctaa acgtctactt cttccagtat tgaccttgat aaaacaacaa tcaggatccc
     3061 gttttaatac tctatgaact gtgtcaatac taacatgtaa agttgcagca atatcagcta
     3121 atgtactccc atttaatctc atttgctttg caaattgata acgccgataa gcatcaatat
     3181 tcttggaatc catagacaat aatacttttc ttaccatgta tttcctccaa agattggatt
     3241 ttaatgggcc atcatttctt gagcaatctc atgctcagat aatgaagctg gatagaaagt
     3301 aggccaatta tgcatttcat tataaagttt ttcttgactt tctccaccaa agaaaatatg
     3361 gaaatgctct gccttacttg gtgcaattcc atgatcgcta aattgaatat atttaaaacg
     3421 accagcacct gcatcatcag tttcaaaatt aaaacgaaca ccacggtttc ctttttcata
     3481 attcaaaatt ttgtatccaa catatttgta tgtgtattga tgatgttcac cgttgactac
     3541 aaaatcaatg gtgttgtctt taatgttgat agtatcaata tctgtcttat agccagtagt
     3601 ataataatct ttgtactcgg cagctgtcat gtctttattg agttttgctt tgtaatcgaa
     3661 aacttcatcc aaaattccat cttttaacaa cggataaaca gattgccatt cgcctgtata
     3721 atcactcaat tcgcgatcct tcacatcggc atctttaaag tacccattag caactgtctt
     3781 ttcatccttc gcttcttttt ctggaactac ctcttcacca gcaaccatag ttgttttctc
     3841 taacgctttt agattgtctt ccattactga aacataatct tcaccattat ccatttgttc
     3901 ttttgtaaga ctttcaagag gattcaatac ttccaatttt atcccagctt cattggccaa
     3961 agtttttgcg attttatcat tagcattctt ttcaaagtaa atataattga tcttattttt
     4021 tttaacatac tcttttaatt cagctaatcg accagaagat ggctcttctt caggacttag
     4081 tccagcaata ggaacttgta tcaatccata atccaaagct aaatatccaa aggctgcatg
     4141 ttgtgtcaca aaactttttt gtttcgcttc tttaagacta gtggtatagt cagcatccaa
     4201 ccgtttcagt ttagtcaaat acttttcagc atttgtctca aatacttttg cttttttcgg
     4261 atacaattta actaactgat ccttgatatt tgaaacttcc ttaattgcca ttttgggtga
     4321 aacccacgta tgaggatcta gttcatgatg gtgatcttct tctccgtgat catgatcttc
     4381 ttcttcacta cctggcaata aaatcatgtc ttttgttcct tctactacat taggcccagc
     4441 tttcttccaa ctttctttag cttttggtac ccatgattcc atgttttcgt tatgatatac
     4501 aaaaacatca gcatcatgag cttttgccat atccttagct gagggctcat agtcatgagg
     4561 ttctgatccc gctggaacca ttaaatccac gttcgcttca tcgccgacga tgtttttggt
     4621 gaaatcatac attggataga atgttgttac aatttctaat tttccatctt tatcattcga
     4681 tacttttgca tcattagtgc tggagcaagc tcccaataat cctagagctg ctatcccaat
     4741 aagtcctaca ctgattttct tcattctttt tccttcctta attatgctaa tttcttcatt
     4801 ttattttgtt tatgttcttt gataattttt aatgcccttt ttctagtttt gatattcggg
     4861 cttttaagtc gtcgatatgc actagatata tccgttttct ccattagaaa ttacccctaa
     4921 tcaatactta tcattatttg ctttccgcca gtaatttatc aaaactctgt ttgatccttt
     4981 cttcatccaa catttttcca attagaataa tagtggtttg gtctttttgt cgagtggtgt
     5041 attgaaaatt aacctgttcg tttacccctt gtatggcaac tataaaatct tgatcttgca
     5101 aagcaatcaa tcctttaaaa cgatacaggc tcccttggtt ggttaaaatc agccggtcaa
     5161 ttaattggaa taaaaaatct ttttttatgt cttgatctga agttaagaga attgatttaa
     5221 actcatgttc ttgatgatgt tgatgatagt gtttgccatt atagtgattt tctgatgaat
     5281 cactttcaaa aactaaggga tgattgaatt tttccaaatt ataaaaatcc tttttttgaa
     5341 tcgtttcact atgtgtgaac attcgaatat cggataaagg gttaatactt ttgatttctc
     5401 tctgtaattt ttttagtgca gataaattca agttttgttt tctagacaca aaaatgcgat
     5461 ctgctatgac aagttgctta agggcttgtg gttctctttc aataagttca aaactgtcac
     5521 tatcaataac agtcaataaa ctatcgatat atactcttcc tcttagttga ggcgctgtta
     5581 aaagtgtttg aatgattggt tggggatctg ctatacctgt agtttcaatg atgatttgtg
     5641 aaattggttg ttgatgttct tctattgttt ccagtaaaga aatcaatatt gctaataaat
     5701 cactgcgaag actgcaacac atacatccat tatttagttg taacacccgt tcctcgctat
     5761 gaactaataa tttgtgatca atcccaactt caccaaactc attttcaatt ataataattt
     5821 cttccctggg gtagggtgag acttgcaagg cttgattgat tagcgttgtt tttccagccc
     5881 ctaaaaaccc agaaacgatt gtaactggta tgcccatttt gtcacctttt taccacttag
     5941 ctatttcact tctttgaaaa ttgcccgtct gcgcaatttt ggcgaatact tttttaattc
     6001 taaccgttca gggttattcc gtttattttt gctggtgaga tacagacgtt ctccagtttc
     6061 gacacattcc aaaataatat tttgacgcat aaatcttcct ccttcaatac ttatcgatct
     6121 tcaagatatg cttgaatttg ttgtgcatct tcaaaagtta actcgccaga ttccaattgt
     6181 gaaactaaat tttctttcat atcaacaggt atctcccaat aaccatcaat acattgaaat
     6241 aactcctcgt catactcttc aaaaattgga tagattctta aaaaatcatc aaatgataag
     6301 ccttggtcct catagtaatc tttgactaat ccattttcaa ttgcctgttg ggtcggtaaa
     6361 cccgtttcag taataattcc tctctctacc atcgaacgat aaaaagtgca tttatctagc
     6421 gattgctggg tcgtttcaag ataatacttt aatccttctt caactaaagg tgggtaaata
     6481 atttccattt tgattccctc ctaaaagtta tatattaaac tattttgaag ctcctttaat
     6541 tactttcttt ccattatagt acccttttaa agatacatga tggctacgtc tataatcacc
     6601 attcgcttcg tcaaatgaaa tttctggttt tactacagtt tgatgagttc ttcttaaccg
     6661 tttcttcgcc ttagatgttt ttcttgctgg tactgccatg atatttcctc cttttaccaa
     6721 cttgcttttt ttactccagg aatcaaccct tgatgggcaa gttctctaaa cttaatacgg
     6781 gacataccga actttcgcat ataccctctt ggacgaccat ccgtctgatc acgaagcctc
     6841 agacgattcg gatttgaatc tcttggcaat ttagcgagag cggtgcgatc tcctgcggct
     6901 tttaattcct gacgtttatc tgcgtacttt tcaatcatcg ccatttgttt ctttgcttta
     6961 gcaattttcg acttttttgc catatacctt tatactcctt ttattttgat ttagacatct
     7021 caaatcgtaa tcgttacgtt ttgacactga tactttacca acaagagttg ctcttgtcaa
     7081 caataaataa catgaaataa aactcatatg aatagataac tatttgaaaa cgaatttatc
     7141 ggtacataga gataatcaaa tcatttaaaa gaatatgcag tttaagctct gaaaaataag
     7201 aattcacttt tattagtcca atcaaacaca aataccgttt taagtagctt ttcctctact
     7261 taaaacggca atatgaaaga aactataact ttttattcaa tcttttatgt ttaactggtt
     7321 tatcgccaat tttttaaaac tttgattatt ccttatatta taagaataga cattaagtga
     7381 actaattcaa tcatactgat tctttcgtgt attcaaaatt agatgctgtt taataaaatt
     7441 attctttttg aaaaagaatt ataactatat cccaaaaatt gtaggttaat ttaaagccta
     7501 tttcatcatc gtgattgagc ttctttttca tagacgaatt tctcagatag tttagttttc
     7561 tttatgagat ttttgtatat ttctgacctt cgatacaact cactatgagt accttgatca
     7621 actatttttc cttgatccat cactacaatt ttatttacct tttcaataga cttcaagcga
     7681 tgcgatataa ttagtactgt cttatcttgt attaaacgat ttaaactttc ctgaatacgt
     7741 ttttcatttt ccgcatctaa actggcagta atttcatcta aaattaaaat tggcgcattt
     7801 ttcaaaaaag cccgtgctat ggataggcgt tgtcgctctc ctccagaaag tgaacttccg
     7861 ttttctccga ccaatgtatg ataaccgtca ggtagttttt caataaagtc ttcgcagtta
     7921 gctaaacgag ctgctttttt cacttcctca tctgacgctt gactatttcc aatacgtata
     7981 ttttccaaaa tacttgtatt aaacaaagtt acctcttgaa aaacaatagc gattttgcta
     8041 aacaaagaag ctggagatac acgtttaata tcatacccat caattaaaat acagccttca
     8101 tcataatcat acagtctaga cactaatttc aagatactag ttttcccgga accactagca
     8161 cctactaacg cagtcacttc tccttgttta gccgtaaaag agatatgatc taaaattggg
     8221 gtattgttat catatgagaa agagacatct cgcaactcaa tgtcaaaaga tttcagagga
     8281 ctatctgatc cctcttggat actggtttcc ttcatagctc taattcgctg tattttagga
     8341 gctaaataaa atatttcaag cactgcttct ttcatagaat cgaaggaatc ctttattttt
     8401 attgcggcta gtaagtaacc aacgacgtag agtatagtca cttcacccgt taacagtaag
     8461 tgaacaccta ctagaatgac tactgctaaa ctaacatatg agaaaataga ggataaggcc
     8521 atgactgaaa aagtacttaa ttctaccttt aaatgaatcc tttcactctc ttccattttt
     8581 ttgaaaagtc tgtcttgaac ttttttagat agattaaagc tattaatctc ttgctgcaat
     8641 tctatagttt cttgaaattc ttccgagttt tctctcaaag tatcgtaata tttagtattg
     8701 gctttcgttt gggatttctt tgataacaag attaagacaa aactaaataa cgttggcaaa
     8761 ataacagcta atcccatttt gacattacca accaaaagca tcactgaaat aaaagggaaa
     8821 aacagagcca taccgccggc tttaggtatt gcatgactca tcgcatgctc aataccttca
     8881 acgtcagaca tgatagtttg agataaatct gataaattat gttttgaaaa ataggataat
     8941 ggtaaatttg acaaatcgtc tgcaatttgc attcttaaat gcgcactttc tttataagta
     9001 ctattatata agttttcata ctccctatct aacaaccaat acatgaaaag caaagtaacg
     9061 aacgatactg taaaataaag ccaaggcggc tttacatttt ctaaaactaa ttcgtctatc
     9121 agcatcatta taatgaatgc tggtaccata ttaatgcagt atacaaaaaa gctagaaata
     9181 gatgctttaa ccaaatcttc tgcgccctga ttagttaaag cgaatttgtt tttataaaaa
     9241 cttttcattg tcaagcctcc aatcatttgc attctcataa ttagtgacca attgtttata
     9301 cacttgatgt ttagccatta attcattatg atttccacgt tctactattt tcccttctga
     9361 caagacaaga atttcatcta cagatttaat gctggataat cgatgagcta tcataataac
     9421 tgttttttct ttcatcagat gttgaaaagc cttttgtagt tcatactcat tatctggatc
     9481 aatagatgca ctggcttcat ccataataac aatctttgca tcttttaaaa ttgctctggc
     9541 aatagctata cgttgttttt ctcctcctga cagataaact cctttactac caattaaggt
     9601 atgctctctt tcgggaaatt tttctaaaat cgattcgcaa ccggctaatc ttagcgcgtc
     9661 aaatacttta cttgctggcg cttgaggatt tgctatagaa acgttatcgt aaatactctg
     9721 attgaatagt ttcgtatctt gaaagacaaa agcaattgtg ttaataattg tgtcttttga
     9781 atagtgattt aaaggcttac caccaatttt aatctgtccc ttatccacat tgtagaaacc
     9841 tgaaattagt ttagctatcg tactctttcc gcttcctgat tttcctacta atgcataaat
     9901 tcttttttgt tctaaaacta agttgaaatt ttctaaaaca cactcttctt tgtatgaaaa
     9961 gctgacattt tcaaatgcaa tgtcacaatt tttaatctgt gtctcgttcc catattttag
    10021 ttggtcttgc ttcatttcta aataaaggga gtctagtgtt tcgactgcat atcttgcttc
    10081 aaacaagtgc atacttacat acattattct catgaaagaa acaaaaataa ttccacttaa
    10141 aaaaaacaac atcaacaagt caacagctaa tatcttggga gtggtaagag aagttataaa
    10201 aaaggttata ggaactatca aaatgcaaac tatgccaaaa aatagccatt gaaaaagaac
    10261 aaaaggtgtt ttcccactca aagaatactt atatgcaaac ttagcataat cgttaattgc
    10321 tttattcaag gctttaaatg atgttacttt agcaccaaat atctttataa cttgaattcc
    10381 tcggatatat tctaccgttt cagagcttaa cacatctaac gattcttgat atttttgaat
    10441 aaaatttgta ttctctgtca ttttctttaa caagaataca ccaattacta acatggcgat
    10501 tagaaccaat ccaattttaa aattaatgaa aaagcctaag gcaactgata aaacaggtgt
    10561 tactatggct tgagcactat ctggaatcaa gtgagcaatt gccatatgtg ttttagccgc
    10621 gttgtcatca attgtctttc gaatatatcc cgaagaattt aaatcaaaaa aacgaaaact
    10681 tgcttctgtt aaaccgctaa ttcccttttt tctcaaatta gtctctaagc gaaacccaag
    10741 gatatgagaa aataagccag aaaaaatata aaataaagct cctaaagtta accaagcaac
    10801 agtttgaacc gcataactct tagctaaata ttcatctcct acaataatta gagacttaag
    10861 aaatttgtaa atactgtaat atccatagac tgttaatacg acagaaaaat cagaaaataa
    10921 agtggctaac catccgtagt atttcatttt agggacataa ctaaataatt ttttgtatac
    10981 tttcattttt tcctcccccg cattcaattt cttctcttat tcgtttacaa ctgttaatgc
    11041 tttccgtaat agcgtaaatt agagatgtac aaacagatag aaggtaaact acgttctatt
    11101 ttttgtattt ttttaagact tttcttccaa tcaatacacc aatcatagat aaacttgcta
    11161 caactaacac tgaaatgatt ccccatatac ctaccgcata gtggatcatt gcatccattg
    11221 ttgcagcaga ttgtccttta tctatccaat cttgacgaaa acgatctgta aagaaccata
    11281 taggaatcca ctgtccggca gcactgccta attgcattag agcccaagac gcaacttgag
    11341 gaattgctcg atcatatcca aatacagata gaataatatc agctaagatc gccatactaa
    11401 tcgaactgat aaaccaaggt aagtacgccc cgcccattaa cgtaaaaaat aacaataaaa
    11461 caattgtgta aatagtaaaa attccttttc tagggtagtt atagctaata taaacaaata
    11521 tgattccaga taataatcca aaaatccctg gggatatgct gtgaccaaac gccccaccaa
    11581 tactactaac cattattgaa aaaaagtaaa ttactaggga aataactgta gctaaaccaa
    11641 taaaaatata atcttttgtt ttcatatata ttctcctcaa ttctatttcg ttatgtcatt
    11701 aaaaatctgt tctatcaaag ttgaatcttc tactctaagt tttatatctt tttttattcc
    11761 accatttttt aaataaataa ttctattgca tgttttataa agaaattccg gatcatgagt
    11821 ggctataact actgcaccct tttcttttaa gtctttaata aggtcagcaa tcctttccat
    11881 attaatataa tccaacccac ttgtaggttc atcaaataga aataaattag tttttgacaa
    11941 tgaagctata gaaaccaata atctttgttt ttctccaccg gataaatcaa acggtaattg
    12001 atctttcatt ttataaagtt ccattttttt taaaattttt tcttgctgat ctttactaac
    12061 tatttcattc cccagggcta actcgctcct cactgattct gtgaaaaatt ggtaatccat
    12121 ttcttgcatt actaaaaacg gcgatgacaa atttttttta ttttcgatta tctttccttt
    12181 ggtagggcga ataatcttca tgagtattct tagtaaggtt gttttcccca caccgttttt
    12241 ccctaccaaa ccaataacat ctccaacttt gacatcaaaa tttatgtctt ttagaatatt
    12301 ttcataagta atttcttcaa cttttaatac atctgcactt accttttcga tatagggaac
    12361 ttcaatcgaa ctattaaaaa tgtcaaattt ccttaatgcg ttattgtttt cagtttttaa
    12421 caactgaata gttgtagttt ttacagttct atttttaaca aatagaactt gatcaattaa
    12481 ttctttcaaa tagtaaaaac gatgttcagc tataacaata gtaaatcctt tattttttaa
    12541 atcacaaagt aagtcagcaa gtttcttagt cgctttgaag tccaaatttg aagacggttc
    12601 atccaatata attaatttag agtttagcga taaactacat ccaatagaaa ttttctgccg
    12661 ttcaccactt gatagcgaaa aaatgttttt atctaatata tttgaaagat ctaataattc
    12721 agctaattca ttgattcttt tatccatatg ctcttttgta aatccaaagt tttccatcga
    12781 aaatactaac tcactcgttg tatttgttgt aaagaattgt cctctaggat cttgacttac
    12841 gtagccaata ttttttgata tactttcttg tgttaaggat tgtagcgagt tattttcaaa
    12901 taaaatttcc ccaaccaatt ttccgtcata aagtcttgga ataagtccat tcattaactt
    12961 taatagtgtg gatttcccac tcccagaaga accagtaatc aatgtaagac tacctttttt
    13021 tattgaaaaa ttaaggtcaa caagcgcttg ttccttaaca tccttatatg aaaaagaaac
    13081 attgttaaat tcaaacaatt ctttcacctc aaatcaaaat acttaagaaa aaaatagtaa
    13141 cggaaaacag gatgatatag tctaataagc tcatgtttgt tttatagtaa gaacttcttt
    13201 tcacaggaga ttctatcccc tttgttattg cattagcggt catttcttca gcaatcatag
    13261 ttgcacgtat aagaacggga actaacagaa attccaaata ttgcagaggc tgaaaaaaag
    13321 acaagcgaat gtttctcatt tttaaagatt ctttaatcat tcttatttca gaacgataag
    13381 tcggaaagaa tctgatcact actgtaatac ttaacattat ttttttcggt aaatgtattt
    13441 tttctaaaga ggataataat tcattgctag tatagacact aaccaacaac catgagatca
    13501 ttacgaccgg caacattcga aatacaatat ataaggatgt cccaaaaaat atagtaaaaa
    13561 actctatctt gatagcatca ataattttca ttactaaaaa cagaactgaa taaatcaaga
    13621 tgaaataaat acatgtcttt attttacgaa acatcaatac taaaactgta gcaataatga
    13681 aacaccccac ttgaaatata tttccctttc ctaaaaaaat aatgaccgga aaaaaaatat
    13741 ttaaagtaat caaggttaac ggattgattt ttgacactct ttcacctcca aaaaataaga
    13801 tcgcactgtt atcttaagct gataaaaatg caccgttatt cagataacag tgcgatctta
    13861 tcaaaaagca taactgagaa caattctcaa tgcaagcttt tttttaatta actcagtaat
    13921 tcaatccacc caggaacaga aaattgatac aatcttctgg ttaatagcat agtcttttct
    13981 tttgaatagc tatgcttcaa tatatcatag aatgtagcgt agaatgagta aatgtagaag
    14041 tggatttctt cttcattaaa gactaaattt tgattgatca ttccgatagt ctttaactta
    14101 tctagacaac agactgtctt ttttgtcaca aatgatgtaa ttttttcaac aatatcttga
    14161 tatttggttc cagctgattg gaataaaacc aaatcgaata tacgtttatt ctcaaaaata
    14221 taattaataa atggatctaa ttcattgaag ttctctctcc aacttttctc tatatctata
    14281 ttttctaagt ttaagaggta tttggtaaac tcttcattat acatattaat aagcgcatta
    14341 atatgcggct gaataatttc ttcaaataac gcttccttag aagtaaaatg tttataaaaa
    14401 gcacccgtag ttacgcctgc ggtcaagcac agttgcctta tcttagtttg ctgaaatcct
    14461 actttttcaa aatggcttag tccactttct attaattttt ggtgagttgt aatataatct
    14521 ctggtttcta tgggtacttc ctcctaataa gatgaattaa ctatattatc ttacacagga
    14581 cttgaagttt caatcgttaa cgtctattac tgaactcatt ccttagagct aaactaatga
    14641 gaaagagcaa aataactgat acaaaaagca atcctacaag aattaataga ctcatctaaa
    14701 tcattcacct tttctaaaat aatccgagaa agatttaaaa aaattatatc cagctattag
    14761 gataagtcct atcataatca ctccaatagc cattttctta tcctccctaa aaataaagcc
    14821 ttttacttaa aacctttttt tgtaatcgtc aataaatttt ttccctttat cagttaattg
    14881 aaatcctata tctaaatagt cctcatatct aagtttagtc agcatctcac ttacagatgc
    14941 tggactaaca cttagaactt ttgctaaatc attatttttt aatttctttt ttctttttga
    15001 taattcctga attgctagta aataaattag tatagtagtt gttttaattt gtaccatacc
    15061 taccctccaa attatttcga aaggccttca gcaattttat ccaggttcca tttcatcatc
    15121 gcatagtaac tatcaccatc ttgtcctttt tctgcaattg aatcagtaaa aatcgttgaa
    15181 tagataggaa tattggtatc ttttgatact gttttcattg gtctatcgtc cacactgctt
    15241 tctacgaata aggagggaac ttttgttgtg cgtaattttt caactaagtg ttttatttga
    15301 tctggtgttc cttcttcttc agtgttgatt tcccaaatgt aagcagaagg cacattatac
    15361 gctttcgaga aatatttaaa gcatccttca cttgtaacaa tcattttttt atcattcgga
    15421 attgaagcaa atttagattt agcttctttg tctagagaat ccaatttttc aatatactta
    15481 tctagatttt ctttatagaa ttttttatta tcaggatctt tttccgctaa ctgtttttca
    15541 atatttttag cgtaaataat accgttttct aaatttaacc aagcatgggg atcttccttc
    15601 cctttctcac tctgaccctc taagtaaata acatctatgc catcacttgc tgcaaaatag
    15661 tctttgtttt cctctttgtt cgcatttttt actaattttg taaaccaagc atttcctcca
    15721 gtttccaagt taacaccgtt ataaaaaatc aaatctgctt ttgaagtttt ttgaacatct
    15781 tcaggcaaag gttcatattc gtggggatct ttcccaatag gtacgatact gtgtaaatca
    15841 attttatctt ttgctatatt ttcagtaata tctgctaaaa tcgaattagt aactactacc
    15901 tttaattttt cgttcgaagc cactgtatct ttgtctttac tatttgttga acatgccccg
    15961 aatataaaaa gagcaatcaa aatccccaga cttcccagta ctgctttcca ttttctcatc
    16021 ataactttct ctccttcttt cttaaaaatt tttgttttgg tgagattaga aaactaatta
    16081 caaataagaa tgccgctgtt aaaacaatac ttgatcctgc tgctatgtta aaactataac
    16141 caatgaacaa acctagcaat gatgagcacg ctccgactgt cgccgaaata aatatcatag
    16201 tttttaaact gtttgcatat aaatatgctg tagcagctgg tgtaattaac attgcaacaa
    16261 ttaatatagt tcccacactt tgcatagctg ttactgctac taaagttaat agaatcatta
    16321 atagataatg ataaaaactg acattcatcc ccattgcttt tgccattaat ggatcaaaag
    16381 aagtgattaa taattcttta aaaaatagaa caactgtaac caacactaaa acagaaacgc
    16441 caattgtaat ccacttatca atatcttgta cagctaaaat atttccaaat aagatatgaa
    16501 ataaatccgt tgagctattt gcaactccta taaggataac tcctaatgct aaaaaagaac
    16561 tgaaagtaat accaattgct gtatctcctt ttattacact attgcttttt ataaaagtaa
    16621 taatgataga agctaataga ccaaatacaa tcgcaccaat aaaaaaatta atacctaaaa
    16681 tataagaaat tgctactcct ggcaaaacag catgagaaat agcatcaccc ataagagaca
    16741 ttcctcttaa aataataaaa caacctactg ttccagaaac aatccctata actacagatg
    16801 taattagagc attctgtaaa aagtgaaagt catacagacc attaataaaa ttttgaagca
    16861 tattattccc cttgtatatt agagttaggg ttttaacctt ccggttttga ttggataacc
    16921 ctatgaacca tcgttatttt aatttggtat taactcattt cttttgagat aatatattta
    16981 ttggattgag aggctaagcc gttcccccct ctccaataaa tatagtatct ccataggctt
    17041 tttttaaatt ctcttcatta aatacagatt ctacacttcc gtgagcaatt agatttcgat
    17101 tcaaaagaat aatattatca aaatacttct ttaccttgct taaatcatga tgcacaatta
    17161 gcactgtttt tccctgatgt ttcaattctt ttaacgtatc cataattatt cgttcactaa
    17221 ctaagtctat ccctacaaat ggttcatcta aaaatatgaa atccgcattt tgagcaagac
    17281 atcttgctaa aagtacacgt tgaaattgtc ctccagataa ttctcctatt tgatgattag
    17341 aatattttcc catatcgacc ttttccaaag cctttgaaac tctttgccat tctgcatttt
    17401 taattctttg aaaaactttc attccggcat atgttcccat agatacacat tcttttactt
    17461 ttattggaaa agtaaaatca atatcagctt tttgctctac atatgcaatc ttcaccagtt
    17521 ttttattgat atcttcatta ttaatggaga cctctccctg atgagggata atatttaaca
    17581 ttgctttaag caatgtagat tttccagcac catttggacc aataatacct gttaaagcac
    17641 cttcttgtat ttttaaagaa atctcgttca atgctaaaaa atcgttataa caaacattaa
    17701 gctttttcac ttctaacata actttctcct tctaaatgat aatgattatc acgatcatat
    17761 ggtatacaaa aattatattt tagtcaaccc taaattttat aatttagatt tttttctgtt
    17821 gttgaagatt aggatgtcaa aattatttga aactactcgt atagtaaata gtaagtaatt
    17881 ttcaaaacac gaagaaaaac aaaaagaaaa aaggaaacgc cgaagcattt cctttcagtg
    17941 atttaatgag aacaacacga tttgacttag agtctcaaaa agtcgaatag gtacactcat
    18001 ttaagtgtct ccattcgact ttttggggat gagctggcta ttgaggtccg cttaccctat
    18061 tgaataaata caagattggg tatttctaat aagtccgccc ttcatattgg ggtgatcggg
    18121 gcgaacatgc catcttttca tataccacct cctcgacact caataatcaa agattctcgt
    18181 taatcttctt cgatgcttgc aatgatgttt tcaatattca tataacgaaa atcttcttgt
    18241 ttgatataaa cgatctgagc ggtacatttt tcctttttga tgatttcttt tactttttcc
    18301 tcagaacgtg cgacctgcgg cgttaaaagt aaaacgtcat agtctttata attcagtttt
    18361 tcttcaacgt ttcggatcgt tgcataatct atctcatacg atagattttt ggctctgatc
    18421 gtttcaagca tttttgatac gagcagcgac gcactgattc cggtctcgca aataatcaat
    18481 agatccttat gcataatacc ctcctgtttt tagcctgcaa agccaattgg tgaaaatgcc
    18541 acataagtta aaataacaat gactaccaat gctatagcag tcaccttggt gtatttccaa
    18601 ggctcgatat cgacctttgt atcgaatgag ttcaacacaa attctccatc aggtttcaat
    18661 ttggaaacca ccatcaaaat gataatgtcc agaacgaata atgccgtcaa aacataaagg
    18721 aaatggatat tttggaaaag catttttgat agtgcataca agacgatatg cacggcaatc
    18781 gtcgtttttg ccccaaaagc agttgctttt tttgaataaa aggcaaagag aatgatcacg
    18841 agcagcggca tgttgtatag tccattgaat tcttgaacga actgatacaa tccagccggt
    18901 gcaaaagaaa tgaatggcgc tacgataatg gagataatcc ccacaacgac cgttactgtt
    18961 ttaccagcac gagcgatttg cttatcattt gctttcttat tgataagcgg tttataaaaa
    19021 tccagtgaaa acaaggtcga tgtcgcgttc aaagctcccg caaatgatga taagatcgcg
    19081 ccaaagataa cagcggcgaa aataccaaag atcattttag gcaatacttc actgactaaa
    19141 aaaggatacg cgttatctgg atttgacatt aaactatcgc caaaaatatt gcgagcgacg
    19201 atccctggaa agacaaggaa taacgcacct aagattttga agacgccgac taggaaagcg
    19261 cccttttgtg cctcagctaa attcttgccg gacaatgctt tttggacgat catttgattg
    19321 gtacaccaga aatacagatt gttaaacagc atccctgtaa atagtgtcgg ccatggaaca
    19381 acagccgagt cgatcgatcc cacagagtta agcagccatg gcgtgtttac cacaacgtgt
    19441 tcgatcccgc caacaaagct gccgtcacca agcttcatta agcctaaaat tgggaccagc
    19501 agaccacaag ccagcagacc gactccataa atggagtcac tattggcact taacgacaag
    19561 ccgccgatca gcagatagag gatgccgaca ataccaattg ccatcgctac aattatgatc
    19621 gcgacgatcg gctggacgcc cagcagctca tcaatatgga agatcttatt gaacaccagc
    19681 gatccagaat aaagaaccac gggtaagaaa gaagtcatat aggtcacaat aaataagatc
    19741 gaaataattc gtttggtaac cgtatcataa cgaatctcga tgaaatctga gattgtattg
    19801 atgccgtatt taaaatattt tggcaagaat atcacagcca aagctacgat cgcaatcgta
    19861 gcagttactt cccaggccat tacttccatc cctgcatgat aactttgacc attttgtccg
    19921 actatttgtt cggtggacag gttcgtcatg atgatcgtgc cggcgattgg caaagcagtt
    19981 aagctccgcc ctcccatgaa gaacccttct gatccagaag tatcgacact tctggatcta
    20041 gaataggcaa ataaccatac acagatgaca atcactagga aactgattat agaaaaaaga
    20101 ttcatcgtct agctcctttt caatagatta aagaccggtg acctctcgaa tgtagcttct
    20161 ggctttgact gcatattcaa atggattggc tttagccggg tcctgttctg cttcaactac
    20221 gatccagcct tcatagtcgc tggcatggat tgctgtccag attggagtga aatcaatcat
    20281 cccatcacca ggaaccgtga agacgccttt cttcacgccg tttaagaagc tgtctttttc
    20341 cttttctact tcttcagccg tttcttgacg aatatctttg aaatggatgt gtttgatacg
    20401 atcttgatga cggttgtaaa ccgcgattgg attttcacca gaaaagacca aatgtcccgt
    20461 atcgaacaat aatttaactt tgtctggatc agtcatttcc attaaacgat cgatttcttc
    20521 cgtcgtttga acgccggtac ccatgtgatg atggtaaacg atctccatat ccttttcgtg
    20581 ggccaaatcc cctaattttt ctaatccttc cgccagtttt ttccactctt catcagtgaa
    20641 aactggttta tcatcaaaga ctggcgctgt cataatccct tggatactat gaccctgctc
    20701 agctactacg atgactttag cgcccatcgc atgtaagaaa tcccgatgtg ctttgaaggc
    20761 ttcggcagtt tcttcaaaag gtttagttgt cagaaacgca ctgaaccatg cactggcaac
    20821 ctttaagccg cgaatattca aatattgttc caacacctct ggatcttttg gatatttgtt
    20881 gccaatctct gttccggtga acccagcaag ggccatttca ctgatacatt gttcaaaggt
    20941 attttcacta ccaagctcgg gcatatcatc attggtccac gcgattggtg cgatggctaa
    21001 ttgaatttgt ttggtcatat tctcacactc ctgcttattt ttttagttgt actaaactct
    21061 tcgtatcgaa tgattcttga caagccaacg cgatcttagt tgattgcaat ccatcttctg
    21121 cattaaccgc tggttgacgt ttttcttgaa tacatgagac aaattcttgg gcttcacgaa
    21181 tgaaggcttc tctgaatcgt tctgggaagt tttgtgaaca tggacggatg atcccttgat
    21241 cgttcatgat cgtcacaaga tttttctcag gcgcttgcgc tacccgcagc attcctttgg
    21301 ttcccatgat ctctgtttcg acatgatagc catgggctgc gtttctgccg gcaactagta
    21361 aagctattgt tttatcgctc aggctcatca ttgcggctcc agtttcaagc tcaccagcct
    21421 gatccaattc tggatacgcc gcatttttcc caatcgccca cacttgatcg acttcttggc
    21481 cggtaaacca gcgaaccaaa tcaatatcat ggatcgccat atctaagaac aagccgccgc
    21541 tgtcgttatt catggcaaat ttgacaaagc tttccaagcc ttcgctcgga tcgatcccat
    21601 aacaacgaat cagtgtgatt tcgccgattt cgccatcttc taccattttc ttcgcatact
    21661 gatacgactc atcatagcgg cgcatgaagc ctaacatgaa gacctgttca ggatgctctt
    21721 cgatgacttt ttgggtcttt ttaatatctt caacttcaag gcctaatggt ttttcagtaa
    21781 agacatgctt gccattttcc atcgccaagc ggacttggtc acagtgaaag cctgatggtg
    21841 aaacaataaa gactgcatcg atcacggaac ttttgaccat ttcttcatag ctggaaaaag
    21901 tatgcttgac gcctaatttc ttctgtgcga atgctaattc tgtttcaact aaactgcacg
    21961 cagccgtaag ctcacaacaa ctgacacctg ttgctaaatt ttgtgcatgt acttttccta
    22021 atcttcctaa accgacgatg cctactctaa tgttgttcat gaaatacctc cgttttctga
    22081 gaattactca ttgtatagtg ctggtcgttc atccatttga atcattactt ttgtttttgt
    22141 ttcacgagac tcatgacaag ccatcatggt gatcgctgct acatacccat cccaagcggt
    22201 aggaccgaca accgcatcat ttttgatcga atcgatccac agcttgaatt cataatcata
    22261 ggcatccaaa aagcgggtct gccaatctgg tgaaacaggt gtgtaatttt tttcttccga
    22321 tttaattcgt gtataggctg ggtccgtcat tcctacttct gccgtttcga aaaccacatc
    22381 acatttaata tcgtagccaa agcggcaatt cacaaagatt tccaaatcaa tcgttacacc
    22441 ggattttgtt tgaaagatca gcagctgcgg atcatgcaaa tcggcatggg agttgctggt
    22501 ttttttcggc aagatcattt gaacagattc ataatcttca tttagcaacc agcgaagcac
    22561 atcaatctca tgaatcgcgg tgttgctgat cgccatcggt gttgtgtagt tttcatctgc
    22621 gctaggattt ctatgggcac aatgcatcat taatgcttct ccgtattgct tcgattcgat
    22681 tgcttctttc agttcaatgt agccgcggtc atagcggcgc atgaagccaa cctgaaccaa
    22741 tttcttccca taagcaactt ccgcatcaac gatccggcgg cagcctgctg catccatcgc
    22801 taatggcttt tcgcagaaaa catatttccc atgcttgatt gcttctaaca catactgctc
    22861 atgggtcggg tcccaagaag tcacaacaac gacatccaca tcttcagaag cgatcaatgc
    22921 ttctccggtt tcaaagaatt tagcatcgac acgtttggca acctctttag cagcttttac
    22981 attgatatct gagactgctg tcacataagc cccttgtgtt ttctgattga tcctgtcgat
    23041 atgctcgatg ccgattgcac ctgttccgat aacgcctact tttactctca attacttatc
    23101 cccctgttct gttgtttaag cacacatagt atgcgcttac aaaattattc ctgtgtagct
    23161 tttattctta aaaggtagag ccctctcttt gctccgcctt ttactaccca taagcttgta
    23221 cgtataattc cttaatttga ctatgactag gcaccttttt attattttgc ggacttccgc
    23281 tggtcaacgc atccgcggcc attttgtcta aggctgccat aaacgcttgc tgatcaatac
    23341 cataatctcg aatcgttggg atcgacagct gctcacagag ctcttggatt tttttaatca
    23401 gataatccgc agcctcttcc agcgataaat cttcggctgc cggataaata aatctggcga
    23461 tcgtcgctaa ctcagtctga atagcatcct ttgagaattc aaaaaagacc ggcaataaca
    23521 tcgcatttga tatcccatga ggaatgtgaa aaagtgcccc caccggtcga ctcattccat
    23581 gaatcaatgt gactgacgca ttcgaaaagg caatccccgc ctccgttgaa gctaagctca
    23641 tttgttctct ggcttcagtg tcgtcgctat tttcatatgc ctgagaaagc gtgttcaaaa
    23701 ttttcttcat cgcttccagt gaaaatagtt gcgttacttc attggctttg actgaaacat
    23761 aagattccaa agcgtgacat agcgcatcga ctcccgttgc cgcggttact tttggtggta
    23821 cagttttcgt aaaatcagag tcaacgattg agatctgtgg catgaaaacg agatccttca
    23881 tcatcatttt gactgctgtt tttgtattgg taataactgc cacatcggtt acttccgaac
    23941 cagttccagc agttgttgac actgcaatca gcggaatgcc cgcttgcagc tgatcaaaat
    24001 ttcttgcatg aatcgcttcg agacttaatt gattagcagc caaaacggct accaccttgg
    24061 ctgtatccag acagctgccg ccaccaaaag caatgatatg atcacattga tgcttctgaa
    24121 aaagcttcag cgcatcataa acattttctt gcgtcggttc ggcttgaatg tctgtatact
    24181 ccgctaattg atcatctgtt tttcccaaca gctccttgat cttttgaaga tgcccggtct
    24241 gttccataac cgtatcacta atgattaacg gcttgttgcc tagttgttct gattcttcca
    24301 taagaaattg gattgttccg ccgccaattc ttaaattggc aggcattttc agttgaatat
    24361 tcacagaaaa aacctctctc tggcttatag cctataacag aatcctcgaa gctctttaac
    24421 agagcttcga agcaaatctt taaatctcac tttgcgcata acgtgctgta acaactttcc
    24481 gatgggtata aaactcaaca ctgtctttgc cattggcatg caaatcacca aagaaagagt
    24541 gcttccagcc tgaaaaggca aaccaagcca tcggtgcagg caccccaaga ttgaccccca
    24601 gcatcccagc atcaacattt tctctgaagt agcgaatcgc tgatgcgttg ttggtataca
    24661 gacacgaacc attcgcaaat tcagattcat tcgtcagctc aacggctgcc atcaaatctt
    24721 ttacccgcac gattgatgcg accggggcaa aaatctcatc ctgccagatc ttcatatcca
    24781 tcgtgacatt atcaaaaatc gttggtccaa caaaatagcc tttttctgga atgttttcgc
    24841 gaccatctag taccagtttc gctccttcat cgaccccttt ttggatatag tcaaaggtcc
    24901 gctgctgtgc atcttttcga atcactggtc ctaaaaagac cccttcgtcc aagccattgc
    24961 cgatcttgat cgcttttgcc gcagccttaa atttttcaac aaactgatcc gcgatttcct
    25021 cttccaaaac aatgacggag gctgccatgc agcgttcacc agcagaacca aaagctccgc
    25081 caataaggtc cttgactgcc cgatcaatat tggcatcttt cagcacaaca acatgatttt
    25141 tcgctccagt caacgcttgt acccgtttta gattttttgt tccttcacgg taaacgtatt
    25201 caccgacatt ttttgatcct acaaagctga tgccttttac cagtggatct tgcagcaatg
    25261 tgttgacaac atcgtgggca ccatagacaa cattaaaaac accgctagga aagcctgctt
    25321 tttcaaccag ctcaacaatt ttctccatca acaatggtgt tttttctgaa ggcttcagca
    25381 ccaccgtatt tcctgccgcc agagccatcg ggaagatcca gaagggcacc atcattggaa
    25441 aattaaacgg tgtgatccct ccgattactc cgattgggta acgataattc gtgatctcaa
    25501 tatcagtcgc cgctgttgat aaagaatctc ccatcattaa attggtgatt cccgctgcgt
    25561 gctccacatt ttcgattcct cgttgaactt ctcccagtga ctcggacaat gatttcccat
    25621 tttccagcgt gatcaaacgc gccaattcac ttttatgatc tgttaaaagc tgatgcagct
    25681 tgaacagata acgtccgcga acaggaacag gaactttttt ccaagtttga aacgcagcat
    25741 cggcagcttt aactgcttcg gctgtctctg ctttgctaga caacggtaca ttcgccagcg
    25801 cttcttctgt agccggattc attacttctt caaactctgt tgagcgacta gcgacccaac
    25861 gaccatcaat ataatttctt aaaaatcttg tcatttattt tcctcctaaa aacctaacta
    25921 aacctaacta aactttatgt atctaacagc ttaataacta cttgcgctgc aagatccatt
    25981 cgtgtttctt ttcattgtgg aatttccaaa ttcgctcagg tcccgccatc acatttaaat
    26041 agtaggaagt atacccgtca ggtaccccaa ctggatgata gcccttagga acttcgacga
    26101 cattttgatg ctctaccgtc atcgtttcat ctagtgaacg atcttccgtg tagacccgct
    26161 ggaaaacaaa tccctgctct ggatcaagct catggtaata agtttcctct aaaaaagact
    26221 catgtggcag attctcttga tcgtgcttat gaggaggata gctggaaaaa ttaccgccct
    26281 cggtataaac ttcgacgacc agcaggctgc tggcagtatc gacattatcc ggtaaaatgt
    26341 tgtgaacaag tcgtttattc tgatagagtc cacgttcttc gacggtatta tcctccgcgc
    26401 gaatcacact tatcggcaaa gcttttttcg ctggtgaata gcaaagcgcc aatcggacat
    26461 tgtctgtagt tgccacaagt tcaaacgaat gctccgccgg aagataaata ctgtcggttg
    26521 gaattttttc aaaaacagat tctcgtgttc ctaaatcttc ataccaagtg tctgctgctt
    26581 taaccttaat ttttccagtc aaaatgacga tacagacctc ctgttgactg ccggtttcct
    26641 tgtaagaagc tcccttttcc agctccaaaa tctgaaattc gatataactc aaagcagaat
    26701 tagactgccg aatattttgg atttgtttta ctccaggtgc cagttctttg tttaatggtt
    26761 gatgcttcaa tgtcttttcc aatcaatcca ctccttattt tcaatcacaa aaagcaatat
    26821 ttaatacttt ctgatagtgg caagtttcgc ttgtttggct ttgtaagcct cctgcatggc
    26881 tgctttaccc gatacctctg agataccgac gttccaccag ctgccgtcgt agccttctgt
    26941 catggtttta ggaagtacct ttatctcaat caacgtcgat cgcgtttgct tcttcgcatc
    27001 ctcgaccgct gcttgtaatt ctgcaatcga gcttacacga tacgtttttg caccatagcc
    27061 ttcagcaact ttggcgtaat cgatgttcat gatctcgtcc ttggtatttc tgaattcggt
    27121 tccttggcta gctccgccat tatccatctg taaattattg atacagccga aaccagaatt
    27181 atcgaaaagt acaatgttga tttttttatc atattgcaaa ctagaaacca gctcgctatg
    27241 caacatcaag aaactgccat cgcctactaa ggcatagacc tcttgttctg gtcgagccat
    27301 tcttacgccc atcgcaccag cgatttcata gcccatacaa gaataaccgt attccaaatg
    27361 ataggtattc tccttcacag ggttccataa gcgctgcata tcccccggca atgaaccggc
    27421 tgaaccgatc acgatcgcat cctcatcaac aaaatcattt aaatgaatga agacctctgt
    27481 ttgcgtcaaa ttcgtagcca acgcatcagc atattcattc aagatttctt gagaaaaatg
    27541 atctttgatt tctggcgtaa aatctttacg cttaaaatgc gcggctttta accgatttcg
    27601 ctcactgatc cattcttcct tcagttcatc gatcttctcg ccataggcgg tttggtaacc
    27661 ttcgatcatt ggggcaagct gctccaaggt cgctttcgca tcaccaacca cttgaaaggc
    27721 ttccagctta gcggcctgtt gtctgcttac gttaatattc atcattttcg cttgttcaaa
    27781 attaaaaata cttttagaag ctgaggtaaa atcagtgtag cgtgtgccga tccctatgat
    27841 cagatccgct tgctgcacag ctttattagc agccgaagtt cccaaaatcc ccattccgcc
    27901 gagattgttt ttaaaatccc acgaaacagt cgatttacct gcgtgcgttt caactagcgg
    27961 aatctgacaa tctcttgaga tttgctcaag aatctcacca gcctccgaat atttgcagcc
    28021 gcctcctacg atgatgacag gtcgtttact ggcctttatt cgttgactcg cttcagcaag
    28081 ctcacgagcg gtcggaatcc tgcgatcaat ataatgtact ctttttttga aaaattcctc
    28141 cggataggca taggcttctg cctccacgtc ctgcggcaaa caaatcgttg ccggacccat
    28201 cgttgccggg ttcgtcatga cttcaaaggc ccgcaataac gcggacatca actgctctgg
    28261 tcgattgatg cggtcccagt agcgagaaac cgcttgaaat gcatcatttg tcgaaaaggc
    28321 tacactgctt gcatgttcca gctgctgcaa gaccggatca ggctgacgtg tagcaaacgt
    28381 atccgccggt aaaaaaagta cggggatatt atttgcgaat gccgtacccg ctgctgttaa
    28441 cagattggcc gcgcccggtc caacagatgt tgatacggca aaaattttct tgcgtaaatt
    28501 ctgtctggca aaggcagtag ccgcatgggc cattccctgc tcattttttc cgctataact
    28561 tttcaaatgt ccgggctcta attccagcgc ctgaccgatc cccaaaacat ttccgtgacc
    28621 atagacatga aagatacctt caacataagg cgtttctttg ccgtcgatgc tgatgtattg
    28681 ttgattcaaa aattttagta atgcttgtgc tgttgttaaa caaaccgtct tttccatttt
    28741 tttcacctta aatccgagcg atcatttcgc cgtatatttc tttctccaat gtaataaaat
    28801 cttttacctc ctgagtcgtc ggtaaagaat ctgaacagtt attgcttttg accatcattg
    28861 atgcttccgc tgagccaaac tctaaacaat cgatgatctc ccagccttcg aataaaccgt
    28921 ataagaaacc cgagccgtaa ccatctccgc cgccaaagcc tttgcgtgct tccaccggaa
    28981 acggcttgat tgaaaactgc tgctgatcgg ccgtataagc agtcgatcct ttcattccat
    29041 gtttgattac gacaatctca gcaccacaac tgtgccaaaa agcagcgctt tcattatctg
    29101 acatgttagg ctggatcaac ttctcagtta gatcgaactc ttctcttgaa cccatgataa
    29161 tgtctgattc cttagcaatc atcgaatagt aaattgaaat atcatcgttg ttcttccagt
    29221 tataagcacg gtaatcaata tcaaaaataa ttttgacatt attccttttc gccagcatca
    29281 ctgccttgat tacggcttct ctggacgggc tctctgctag tgcagttcct gagattaata
    29341 gtgcttttga ccgtttcaag tagtcctcat caatatcctc aacgctaagc tgcaggtctg
    29401 cggcttgatt tcgatacatc aaaatattac tttcctttga tgagagcatt tcggtaaaag
    29461 tcagccccag cttttcacca tgctgcgctt tgctgatctg agacgtatcg atgccttgtt
    29521 cattgaaata atcgatcaca tattcgccaa actgatcgtc cgagactgtt ccgataaaac
    29581 cagctttcat gccatgttta gtaacaccca cagcaatatt ggcaggagat ccgcccacaa
    29641 atttttcaaa catatggacc tttttcaatg gtttaaactc ttctttgact tgatcattgt
    29701 aggcaggatt aaaatcaata gcgactcttc ctaaaagcag caagtcaaat tcttttgatt
    29761 ggtcaaactt tatataactc aatatatcca cctctttatc cgttcttcta tttctgaggc
    29821 aagtatagca cgccaaaaca aagcgtgcaa tatttatttt agaacgcaat attgcgtgct
    29881 gtttgaaaat gacggcaaaa aaaacgtgaa tcgccttaca tcacgattca cgcctagatt
    29941 aaatattgta ttttgtctta ataattatat cagtaaatag cgcatctttt gtaaccgttt
    30001 tattattttg cagctgattc gctaacatca gtaatgaacg atagccttga acaaatccat
    30061 cttgatcgat tagaaaatca acctgatcgt ttttcagcat ctcgatattg ttcttagtca
    30121 aatcataaat gattacaaag ggccgcttgc tttcatcgat tttttccaac gctcgggcaa
    30181 ttcccgcttg tccgccagaa aagacgacta cgccatctaa gtttgaataa ttctccaagg
    30241 tgttgcttaa aatcttttca acctcagcac tcgaatcaaa gctgctctga acacccgtca
    30301 gctccagttc aggaaacgac tctttcaatt cttcaacaaa gcctgccacc cgtaagctgt
    30361 tgacgctatt tccaaagtag ccggtaatcg ctagaacctt gccctttcct tttgtcagca
    30421 tacctaacaa tcccgcagcc gtttttccgc ttttgaagtt gtccatccca acaaaacact
    30481 gtcgattcgt accaagaata tccgaattaa aggtaatgat gaggatgcct tgatcggtca
    30541 attgattaat tttttctctg ataccatcac tatctactgg catgatcgcg atccctttta
    30601 ctccaagctc caccaatcga tccaacgcct gcagctgggc cgcttcatcg accccgtcta
    30661 cctcttctaa aaaacaatca atgtttcgct tttttaaatc cccgataata gattccagac
    30721 ctgatcggat cggaatcata aaagaagatt tcgccaattg agtgacaatc cccagcttga
    30781 gcgttgtctc ttttatgacc ttctttttct ttggaacata gttcatttcc ttggcgattg
    30841 ctctgattct ttctgcaacc tcaggattga tccttcctcg atcgttcaat gcccgatcca
    30901 ctgtccccct tgacactcct gctttctcag ctatttcttt aattgtaccc gccatcttgc
    30961 ctctcctttt ttcctattgt gtttatttta tcacaaaact taagaaaacc accggagaat
    31021 gaattctccg gtggactttt tgtatgatta accttcttat tgaacatcac tgatcttaaa
    31081 ctctattcca agggcgtttc tataatattc tggatacatc tccttcccac atttttcaca
    31141 ggtaaattgg ggaggattgt tcggatcatt tgcaatatct gcatcatcta aaaattttac
    31201 tacatccatt gggattgatt ctttttcacc acaacttaga catataaatt tcacgttggc
    31261 gttcccaatc ccagcgaatt gctttaaaag ttttttcttt ttcttcttca tactgttgcc
    31321 tgaattcttt ttggtcaatt tttcgattct cctctcgcat aaaccgcttt gcatggttat
    31381 ctttcgcata tttaataact aaccgtccat ttttgcgcac actcatccca acgaattcat
    31441 agtactcacc gcaacaagaa cattctaagg gattttcacc aaattcttcg gttattcttt
    31501 cttcccagga ttttggtctt aaggcctcat tcagattgat aaacagcttt tttactcgtt
    31561 tctgatactc cttcatgacc tttttcatta atgttttgat tctacgacta tagattccat
    31621 agcgacgaat cgttttgaaa ttcttatttt gaatatggcg aatcagtgca gctatgaatt
    31681 cgtctatact catggtcatc gtctctttag tattcgttcg tttgtccatg tagttgaaca
    31741 tgacagtgtc gccatcatac attaagattc ggcttaatgc gatcgggcca cgcttcatat
    31801 aacgactgat gtattggagt agtgacttta gatttgtcct gctcctttta ggagcattta
    31861 cgtaaaaccc ttctccattg ttcttgtacg cagcttgtaa tcttgattga actctttgct
    31921 tgtcccactc acctaacgtt cgtctaatta gtttcaatac agcatttttc cagtattttc
    31981 ttaacataga aaaaggtaaa taatcgtatt cttcccaact tccatctctt gttaagccac
    32041 ccatagtaac aaccatatga acatgcggat taaattctag ttttgatcca aaggtgtgga
    32101 ttgtagctac tatacctgcc ttcacctttc ttttctcaaa ataggcaaga ataatttttg
    32161 cggcttcatc cattagtcct ttcagtaagg aatctcgata gttttccatc ataaagattt
    32221 cccgtaaccc ctcatcgatc gtaaagatta cgtgccgatg tgtcacttta aacatatcat
    32281 tcgccacaac cgctgaccac ctttggcttt cacctgttgc acacgttgga cagaactttc
    32341 cacgacattt caagggaaca aactttgttt gatgacatcc ctcgcagaca aacagtcggt
    32401 atcctttttg aatatctcca caatatctaa acttgtttac ttcccgtttg acgactagcc
    32461 gaatctttcg cccgtatttc tcaacaaagt tatcccaatt atggtgttcg tcaaaaaaga
    32521 ttgcttcgag aatattcgct ttcatagtaa tattttactt gttcaaaaac aaaatgaaaa
    32581 gggctaaaag actggtagtg cagccttttt agcccttaac tttttatact ttctttactg
    32641 tctaaaaaat atgcggcgca cagaacgcgc cgcatatcgg actaagtttt attcaaattt
    32701 gtcttcgcca acacaatttg acgaagagcc ttttctttca ggaagacttc ggtaattttt
    32761 aggagttcta cctattcttt taaccgttta ttctcttttt ctaagcgtgt aatttcattt
    32821 ctgcgaatgg atttcatgac ttcctctacc gagaaaagta cgttatccat atttttcaac
    32881 ttcttgaact catcaataca cgctattaga gcgagcttct agctatagac taacttcttt
    32941 aacagaaaac ccttcttccg ttaccagttt tacagcggta attttaaata tttcatcaaa
    33001 tgaacgtctt ggcataatcc agatcttcgc tttctcttaa tttggtgtca attttagtat
    33061 aacccgtcca acttattttt taactaagtg cccggttttt cttgacaact ccacccaaaa
    33121 gttagatttt ttggtctaag ttttagggtc gcttcattac tagaattcaa caagagatgt
    33181 tttttatttt atttaaatta tggggtcagt ttccatttga ttacaaactt ctttgttttc
    33241 tttttttaaa cgcaataata aaggttttaa tttcagcctt tttaattaga aaatcaattt
    33301 tcccaaatta ttttctttag ctttcttaac gatttctgtc gcaactacca catctaatac
    33361 agccgaacca actgttttga agatcgtgat ttgttctcca cttgtccgac cgatcaattc
    33421 ttcattgatc agttggccta gttccccatg atagctgtct ctttgaatat acccatcttg
    33481 cagcggtgaa atgaagtccc cagcttcttt tagaacaccg tccatggtat caaaaataac
    33541 aacatctgcg gctttgatta tttctcgtgg aatttcacac atttccggtg tgtaagcacc
    33601 gactccattg atgtgcgcac cttttttgac ccactcagca gagaatgtcg cacgttttga
    33661 agtggtcaca cttgtaatga tgtctgctcc ttcaacgcat tcttgactgg tttttgttgg
    33721 gcgcatcgtt acagaaaatt gttgcatcat tttttcagca aattggctgg cacgctcaaa
    33781 atcaatatca aaaatccgaa cttcttctaa tttacgaacg gtcaacattg cttctaattg
    33841 tgactgtgct tgtccgccgg ttccaatcag cgcgccaatt ttagcatctt ctcttgcaag
    33901 aagttctgtt gctgctcctt gaacagcgcc tgttcttaat tgtgtcaaat aagtaccatc
    33961 caaacaagcg gataccatac ccgtttctgg atccaatacg atcatcgttg ctggtacact
    34021 tggtaagttt ttcttgatgt tttcaggata cacggaaacg attttgacac ctagcgcgcc
    34081 ttcgccacct tctacataag caggcatata taagctttgt ccgttgcttt ttggcacatc
    34141 gatattggtg cgaagaggca cacttgcctt tcctgctgaa tacaatgaca gtgctttttt
    34201 gtccgcatcg atggcttctc tcattgaaaa acttttgata atatcttctt ttttaagaaa
    34261 taacatttga atcgctcctt aattagctgc tgtaactttt tttaattgac tgcccaacat
    34321 cgtatatgca ctgtatagta agaccaaaat cacgatccat ttcaggatat aggtatctaa
    34381 agatttgact aggaatacag cgaccaatac ccccaataca ccgaaggttg aagtaaaaag
    34441 tgtgattttt ctgctgtatt cattaaattt aatgaattga acactgccga tcggtactga
    34501 gaaggtacaa gcccccatca taataggaaa ggctgcaact ggattcaagc ctagcaagta
    34561 gacagttacc atcgttaacg cataagaacc aatgccgata ttgttcaatg caccatagac
    34621 aaagagtaaa aatcctgcca agattaattt tccgccatac aattcactgg ctgttccgtt
    34681 tgatggaatc cagttcattt gtcctgcaaa aatcaacaat gcagcaatga ccaatcctat
    34741 gccgacaaac agtttgatcg ttttctctgg taacttcacg acaaatcttg gtcctaaata
    34801 tgcaccgata atttggcaga taatacaaac agctagtgtc ttgatcccta cgttgatggc
    34861 agtgatgtaa gacaatgcca tgacggctac cggtactaca cattgggtat tcaacgttcc
    34921 gggtagtttt ttcatgctga cccatttcaa ctttggataa aggacggttc cgatagcaaa
    34981 gtcggaaacg ccaaatgttg aaagaaagaa gatgataaat gacgtgaagg gcaacagacg
    35041 catatcccct ggttcttctt tgaaattttg tttgtttttg aaaaggtctc ttagaaagta
    35101 aataataaaa tatagattga ttgcgacaat tagtgtcagc aaaatattcc gaatcataaa
    35161 aaattcctcc tactagtaaa tctttctgat tccctcaaag ccctcaccat attttgcttt
    35221 aagttcttca ggagattctg caatcacttt tagtgtatat gcatcatgtt tgacatattc
    35281 ttttgctaga ttgaccatgg cgtggttttt tgtccaagtg acatcggttg attgcagttc
    35341 ttcgttaatg gtaccaaaca cgacttcttg ctcatcggcg acgatgttga tccatcttgc
    35401 tccaaagtca gcaagtttgt ctgtttcaaa accatgaata taatactttt ctaagggaag
    35461 atcatattca tgggtagaac tgaataaaat cagtacaaac ttatcaactc ttttttcagc
    35521 ttgctgcaat gcctttagta gtgaatccgt gaaattctca tgccaaactt gaatgagcaa
    35581 actttctttt gcgtttttca ccaaatgctc tactttatcc agaacatact cgataccatc
    35641 aactttccac agcatttctt cttcatcttt ttctttgatc atccctagat tcttggtaag
    35701 aaaagaaaga tcatttttaa cacttttttc taatttctct aaaaactcat ttgccgagat
    35761 ggcatgataa agtttaggtt ctgatttatt aacgacaatc aaattctttt ttaatagttt
    35821 ttccaaaata ttatatactt tcgagcgtgg cacacctgag attttactta cttcatatcc
    35881 tgtaagatta cctttttcta atagtgtggt atacactctt gtctccatct ctgaaaaatc
    35941 atattttttc atgatttgaa taatcgtttc cattgattaa tcctcacttt atttttagta
    36001 gctactacgg taacaactat aaattctttt tgattatctg tcaagagtta gttcaataac
    36061 acacatataa taattagctt aaaaacaatc tttttattaa aaagaaacat tataatatga
    36121 atgtttgtga aaaaataata caacttagtc attctttaca acgatttata gaaaaacaac
    36181 ctctttaaat ataaaaggta cgggttactg attacttgta aatctttacg ttccagtttt
    36241 attctttcta aaaaatatac tcacttcttt tcttagactt ctttttttga ttctcctctt
    36301 tatggtcttg tccttttttg ccttcaaaat atttaaataa acccctttca aaagcatttc
    36361 taggttttgg acaataagga atgacttttt tcttcatata aatacctcat ttaactattt
    36421 gatatcatca cattatcaaa aaaaataggt tttttattaa aactatcttg acctttcggg
    36481 agatttcact gtgatttatt tttattccca tacttttttc gttggtcact ttcgtatacc
    36541 taatctaagc gatcatcggc gacggtgctt ttacaacgaa tggcaggaca aaacaagggg
    36601 cggaaaaact tttttgccta tttcataaaa aactttttcc tacagcaatc ccttgttttg
    36661 tcctaccaca cgttgtgaac ccaaagcacg ccgacgaacg cttagctata cgaaaatgac
    36721 ctccaaaaac aaaaaagtga ggtgaataga aacaaaaaga aattgaaatc tcccaaaatg
    36781 tcgtccgcct cggagacaac ttgctgctga aaatggttgg ttaattcatt taagtattaa
    36841 ataagataaa tcatcaaatt aatcggaaaa atagcatgat tggccttatc aaaaataaac
    36901 tgtaaaaact tatatttcta tgagtggtgg aacaatgcta ataccaaatt aatttatcga
    36961 ttacccacct gcacaaacca aagatagcat tattaggaaa cgaatcaatc agcgaatcca
    37021 gcattataat gaggatacta aaaataaagt tttcataatt ataaatccgc cttcctacga
    37081 aaggcaccaa tgaggtgatg aaaaccttag agcgctttct ttcttttttt attccgtgtt
    37141 accctttcct tgcaggtccc tggtacacta cagaacccga ggaggtgagt ggcggggctg
    37201 tatagcggca accccgcata attatatggt gtcggtcatc cgttaaggca tcaatgaatg
    37261 gcgcaaaccc gtagcccgta aacttatctc ttccgaagtt gactcgattt cgccggatga
    37321 ccagtcccta ccaaacctgt actatttttt tacaggaggt tgctatgttc aaaatctttc
    37381 gtaaaaattg ttgtggcttg gatgtccaca aaacgtggat ctatgcgtgt atcggaatca
    37441 cagatgcgaa tggtcgtaca gtgtataagc aagctcgctt ctcttccttt acaaaaggtc
    37501 tcaatgaatt agctgaatgg cttgctaaat acaactgtaa ggatgtgtgt ttggaatctt
    37561 ccggtaaatt ttggattcct gtttttaata ttcttgaaaa aacttgtttt gtcacactgg
    37621 ctcatccgaa gtacaccaaa ccccagaaag gaaataaaac agatcgcaaa gatgcaaaaa
    37681 tggatttgtg atctctttat gtgcgacatg atcaaaccca gttttattcc tggtcttgaa
    37741 atccgccagc ttcgtgatct aatgcgttat cggacgaaac tgaccaacat gaaaacaagt
    37801 gaaaagaatc gtgctttaaa ctgcttaacc gtttctaatc tgaaactaga tgatgttttt
    37861 agtgatgtgt tcggaaaatc atccacttct atcatccaat acttgttaga acatcccggt
    37921 gaatcatttg atgttcgacc atttatccat gcccgttgta aaactcccgt tgaagaaatc
    37981 caagcggcag ttgagggggc gatttctgta cctcaagcga ttaagcttcg ccaatgcctc
    38041 aaccatatta acgagattga gaagcatcga gaagagatca atctagagat tcaagctctt
    38101 gcagaaccct tctcccctgt tttggaacta cttcgtacca taccgggctt agacactcag
    38161 ccaataacag cgattgcgat tctttcagaa ataggaccgg atatgtccgt gtttccaact
    38221 tcgaaacact ttatttcttg ggctggatgc tgtccaagaa acgatcgcag tgcccgacga
    38281 acaaaatcaa cacgtatctc tcgtgcaggg cagtcgttga aaccgctact tgtacagatt
    38341 gccaatgctc ttctcagatc caaagaacat ccagaattta aaaatcgcta tcgtagaatc
    38401 aaagcgaatc gtggacacaa gaaagctatc attgctattt gtaagatgct cctgaccgct
    38461 atctggaatg tcttatcaaa ggtggaaccc tattctgcta aaggatatct ggagccaaaa
    38521 cctgcgaaag aagagaaagt tctgacgcaa tcccaagctc tggacctttt aagaaaacgc
    38581 ggatatacca ttatagatag tgcttaaacc aaagttacgt tgattcttcc taaatagact
    38641 cgttgtcttc catttcaaaa attttgaaaa cggtaggctt atttagtgta ccgttcttca
    38701 ggtatcttca aatagattta ttttcaaact tatttccttt cttcttttac tctcctatag
    38761 cttagcattt aagtgggcac tcgtgtcacc ttgctagata gctatgaatg ccgtaataag
    38821 cctcctagta tataggcaga ctgccaatta taactgattc aatccacatt atgataaaat
    38881 ccaccttttc gtttcatatg tgttgaaata tcacatatgt caaaagtgca cgagtctttt
    38941 cattcagtgc tttgtaaata ctcaaaatta ttccttttca ctttaaaaaa aagataatta
    39001 gtacaaaatc agaaatgagg aaagtattag gatggactag aaaggagttg ttgaatttgt
    39061 tgcaacagat tggtcattta tcaattaaaa attaggtgaa gttttattca tcactgcagc
    39121 tgctgtttcc cagagaataa aacgtctaga agaaactggt cagttactgt attatcaagc
    39181 agcaattgac tacgaacaag cttgattact aaccaaagcg tttatccacc tgttgattaa
    39241 atcaaatcaa aaatcaggtt tttatacgtt cataaaagca atctccagtg tactcgaatg
    39301 tgactgtatc ataggtcctt attcgatgtt aatcaaaatg gggtttcctt ctaccgaatc
    39361 attggacagt tttatcaatg atttatcaaa gtttggacga accaacaccc aaatcgtttt
    39421 ctcgactctt gtttccttaa tggtttatca gttctggaat gtttatcatg cgcttttatt
    39481 aaaatagaaa agagcttttt tcatgcgaca aagcagtgat catttattgg cacatgcaat
    39541 ctttgtggat ccattttttg aaaatacgat atttagtttt gacccaaaaa tctttaaata
    39601 cgtatttgat aatttatctt aacaaccaga taattttatt cagcaccgta ttatctatat
    39661 tgaattctcg gaatcaagat aaattttttt ccgtttttgt ttctttttta atcttcttct
    39721 tgttattaat agtgattagg atgatattcc tatattgatc tttgataatt agatataatt
    39781 tttgaataac gttccctgct attcgagcta gaaatacaag tacgccatga cctagttagc
    39841 gacaactact tagagatttc ttttgttctg ggacaacaaa atgccatgac aatagcagta
    39901 aataatgata cttccgtaag gctcgccgta aactggggag aggtgaaatt cctatgtgct
    39961 tacccaaagc ggttcaaaaa ttttaaacta ttattttagg agatgatttt ataaaatcga
    40021 aagcctactt gaataaaaaa caagcctgtg aatttctagg aattgataat aatactttag
    40081 ataagtggat tcgtgtatat aattttccag taataaaaat tgatggcgta gttcgctttg
    40141 acgtagagga tatcaaagat ttcatgaata cattcaaaaa aaattgaaat tttcttctcg
    40201 tcacatatac tataaatagc gaaatgtact gccttacttt ttaaaggagt gaggaagttt
    40261 gcctacaaag ctttcaaatg ggaaatataa aactaatctg aggtatccaa aaaaattcag
    40321 agaaatcact ggaattgcat cagagaagta tcaaaaagta tttccaacta ggcaattagc
    40381 tattaaggcc gaaagggaaa tgaaaaagaa gattgaaaca gttttaagag aggagaatgc
    40441 aaactcacta gaattgaaag gaaaaataaa atttaaagat ttttatgaat caaaatggct
    40501 tcctagatat gaactaggtc aaactactag gtcaaacagg gctccaagta atgtaacaat
    40561 tagtaatact aaggatatat tccgattgca catccttcca atgtttggtg aatatgcaat
    40621 gaattatctg aattcaaata ctgagataat atcagatgaa ttaacaaaga aatcgaaaga
    40681 atacgcaaat ataaaaatta tcaaaggcta tgttcgctct atgtttgata ttgccgaaat
    40741 actatgttat atcgaattca accgaactac caaaattatt caatgtatta ccgcgcctaa
    40801 gaaaatggct ttagaagaaa aaaggataag ggaagggaat caagcattat catcagaaga
    40861 gctgactgat tggatagaag cagtaaacaa tgacttaaat aatcacttgc ttacacttca
    40921 cgattacaca cttttcatgc ttaccttata tctaggtgat agaaaaagtg agacttatgc
    40981 tctgcaatgg aaatatattg attttaataa aaatacggtt cgtctcaaat atgctttaga
    41041 taaatatcaa agaaagacac atacaaaagg ttggaaagac accgttattc aggcaccaga
    41101 agttgtgatg acattattaa gagaatggaa atcagctcag gctgaacaat tgttgaaact
    41161 aaaaattata caaacacctg accagtacct ctttacctat tccaaacctt ctggagaagt
    41221 aaattgtcct gttcacgctg actacttaaa ctatcgaatc aatgcaataa aaaaaaggca
    41281 tcctgaattg gctcaattat ctccacacaa acttcgtcat acctatgcaa ctatagctag
    41341 ggaaggtgga gcaaatatga atcaaatctc taatgcttta acgcactcgg atatttctac
    41401 tacaaaaata tacgtgaaca cacctgacgt agttgataaa tctgtttttg aagcatttca
    41461 aaaaggattg aataagtgtg attaacatgt gttctcaaat gtgttcttat tataaaaaaa
    41521 gacactttcc aaataattga aaagtgtcct gaaatattga tataaaaaga atattaacgt
    41581 tttgagaatt gtgaagcttt acgagctttt ttaagacctg gttttttacg ttcgaccata
    41641 cgtgcgtcac gagtaagtaa gccagcacgt ttaagagcta aacggaagtc aggatcaact
    41701 tctagcaatg cacgagcgat accatgacgg atagctcctg attgtccagc gtatcctccg
    41761 ccgtttacgt taacaaatac gtcatatgcg ccttttgttt cagtaacagc gaaaggttga
    41821 ttgattactt cacgcaagtc agcatgtgga atgtattctt caacatcttt tttatttaca
    41881 gtaattttgc cagttcctgg tactagacgt acgcgagcaa ctgcgttttt acgacggcct
    41941 gtgccgatat attgaacttg tgccaatgaa attccctcct attagattaa gtttgtgatg
    42001 tctaaaactt ctggttgttg tgctgcatgt ggatgttctg ctccaccgta tacgttcaat
    42061 tttgtaaatt gtttgcggcc taaagtgttt ttaggaagca taccttttac agaagtttcg
    42121 atcaaacgac gagaattttt agcacgcaat tcgccagcag tgattgattt caatcctcct
    42181 gggtagttgc tgtgacggta ataaattttg tcagtcgctt ttttacctgt taatttcact
    42241 ttgtctgcat tgatgacgat gacgaaatcg ccagtatcca aatgaggtgt gaaagttggt
    42301 ttatttttac cacgtagtac agatgcaaca actgttgaaa gacgtcccaa tggaacatca
    42361 gttgcgtcta ctacatacca tttacgttct acttcgcctg gtttggccat atatgttgta
    42421 cgcacgttat tttcctccaa tttaagttct gatgtttgac atacacttcg agtttccggg
    42481 gctctttgtg gggcaaacaa taccatttaa catattaccc gctaatctag tcaatgtcaa
    42541 cgtttttctg cttgattgaa aaatttatat gaaactttac ttcttttctt tcttttatat
    42601 aaaagggtcg tttcctgtca aaatgaaaaa caaaaagagg ctgtgacaaa agtcttgtca
    42661 caacccctta ttttgaataa acggtgttct cctgccaact cctcggtcac aagtcacaat
    42721 atccataaaa cataagaagc tgttttttaa aattgtttct tgtgactcgg agctgaacgg
    42781 caggcttaca acctcttggt aaacggtgtt caatcagctg accctcggta ttagctcaaa
    42841 aaacggcaaa atatgaggaa ctattttgac gtttgtttct ctaatactca ggtcaaaacg
    42901 ctgatttcac aacctctttt tcggtcaagt tcaacaacag tttttacgcg tcgatctctg
    42961 gcgctttgcc caaatgagct tggatcatcc agatacgttt ttctgtggct gttttgaatt
    43021 cgataaagat atcttgtgtt ggatcgtcgc cttcttctcc agaaacttcg attccttttt
    43081 ggtaaagatc tgctagataa cgatagcctg caacaagtgt ttccaaacgt tctggaatag
    43141 tacgatccca gcggccaatt tcatcaggga tttttgtgtt atctgcaaat tcacgcaatg
    43201 tagaataagg tgaaccgtct aatgtgatca agcgttcgga tacttcatct aactgagcgt
    43261 tcaaatcatc catgaactca tccatcattg ggtgcaatgt aaggaaacct ggtcctctca
    43321 tgtaccaatg tgtttggtga acaacaacag acatttggct tagatctgca actaattgat
    43381 ttaatacttc tttggtttgt tggaatttca tctttcttgc ctcctaacat tttcttacac
    43441 ttaaaataat agtagacttt caatgaaaag tacaacaaaa tcatttacct gagaacgatt
    43501 ttcagttcgc ttttttatcc gtaaagccaa tcatttttca gaatcagcta attcttctcg
    43561 cagttgagca aattgctgat aagtctgtgc taaatgcttt tgtgtcgaaa caaataatgg
    43621 atagattttc tgatattttt tatgatcagt ttcttgtact ccaacttgct ctttcaattc
    43681 tatcaattcc tgtgcttcac tgatactttg gatctttccg atgcttctcc agcctaaaat
    43741 cacagctcct aatgcggatg cttctgtgac agtagggaaa acaagcgggt aacccagtac
    43801 atcagccatc atctgacgaa aaatcggaga ttgagcaaac ccgcctgtag cacggatctc
    43861 tgtgagctga ttttcctggc aaactccttt taaaatccga taaaggttga gacagatccc
    43921 ttctagaatc gccttcatca tttctttttc tgtatgttgc cgctgtaaac cgataaaact
    43981 gccaaacgca tctgcgtccc aaagcggtgc tcgctcacca agtaaatagg gatgaaaaat
    44041 cagaccattg gcacccgctg gcacaaaagc gatagccgaa agcagtgcat catatggatt
    44101 ttctcctttt tctttagcag attgaacgac ttcctgtaaa aatgtatgcc ctgcccaatc
    44161 aaagatgcca gcaccattgc tagtcgctcc tcccactacc caatgtttct gatccagaac
    44221 ataacaaaac gtctccattt ctggatggaa ctgaggctga gtagtaataa aacgaagtgc
    44281 tccgctcgtt cctacggtca aagcggcgat cgtatcacca aaagcaccta accctagatt
    44341 agaaagcggc ccgtctgccc ctcccaaaac aaatgatata tcatttggca aacctagttc
    44401 tgctgctgtt tttgatggta gacctgttac ttgataggtg gaaggaaaga cttgcggcaa
    44461 ttgctctatt cgaatgttca agtagtcaag cacttcttct gcccattgga attcatggat
    44521 atcaaaatat ccagttcctg aagctgttcc gtagtcgctg atcagttcgc caaacaatct
    44581 ataaaagata tagtctttga taccgacaaa ataagcagtt tttttgaata attcaggttg
    44641 gctgttttgc agccacttga ttttgttcaa tggtgtcatt ggatgaatcg gcgtcccagt
    44701 ctgccgataa aattctttgc caagcacggt atatttcagt ttttctgctt cgtccgctgc
    44761 tcggttatct gcccaagtga tcattcgaga taacggtgtt ttctcttcat ccaacaatat
    44821 caggccatgc atcgcagagg agaaggagac aaattgaatg attgtgtctg aaggcaactg
    44881 tttaattact tcttttataa cggtgcagac accttgaaag atcaattcct gatcttgttc
    44941 tgccatccct tgtgtttctt ggatcaaagg gtagcgaaaa taagcggatg ctgcttcttg
    45001 ggcttcatct gtgaaagcaa ctgctttggt ttgtgtggtt ccgatatcta atccgattga
    45061 aacgttcatt ttttcacctc aaattttatg tatgtacaaa aatttagttt aagcttgcgt
    45121 atcttggttt tgaaaagggg gagatggcgt gttactttat tttatcattg gttgctgtat
    45181 cggatcattt ttatgtttaa ccgcccagcg gattccgtta ggtcactcta ttatttatcc
    45241 tagatctcat tgtgtcaaat gctgtcgttc gctttcttgg tatgaattga ttccgattct
    45301 ctcgatcatc gtccaacgat ttcgctgccg ctattgcagg tgtcggttgc ccttctacta
    45361 tttgttggca gaagccttat gtggtggtct ttttgcctgg ttctttactt tttcttccgc
    45421 aagaaatttt tcgacgttca tatggatgct ttcggctttg ctattttcgt tgatggattt
    45481 attttacttc atggtccatt ctcacacgct attctgttct tgggcaattt tatggatttt
    45541 ttggttgcag gccggcgtct tccaatggca aagtgtgctg ttaacgttta tcgtaggcgg
    45601 tgtgtgcctt cgctatggtc aatcctatct cggagctggc gatactctgt tgatgctttc
    45661 ttggagtggt ggcttgtctc tagaagagtt gcttcagatc ttgtttcttg ctagtctgtt
    45721 tggaatatcc gttttttctg tttactttat ctgtcataaa aaaaagttgg aaaagctgcc
    45781 tttcattcct tttttgagca gtgcgttgct gattgtcctt catcttaggt attgaaaaaa
    45841 ggttgtgaca aacgacttgt cacaacctct gatatatagt gttctcttac caaatccttg
    45901 tgacttggag ctaggcgaca ggttcacaac ctctccacta aatggtgttc aatcagctga
    45961 ccctcagtat tagctcaaaa tgctggtttt acaacctctg atatacggtg ttccataagt
    46021 agctttttcg gcttcttatt tcttgacgcc ccaatgtcga cgaaggaaac ctgcacgtcc
    46081 agaaccttct cgataaagat tgtaatggag cttgtttttc ttgtaataat cctgatggta
    46141 atcttctgca ggataaaatt cgcttcttgg ttcgattttt gtcacgattg gcttatcgaa
    46201 tcgcccgctg tcttgcaatg cttgttttga acgctctgca atatttcgtt cttcttcatc
    46261 attatagtag atcactggac gataagaatc accgcgatca gcgaactgtc ctaatgcgtc
    46321 agtcggatcg gtttgctgcc aatagatttc caccaactga tcataagaaa taacttctgg
    46381 atcatattcg atctctactg cttctgtatg ccctgttgtg cctgttgtta cttgttcata
    46441 agttggattt ggtacatgcc cgccagtata accggatgtc acagaaatga tccctggctg
    46501 ctcttcaaat ggtttgacca tacaccaaaa gcatccgcct gcgaaaactg ctttttttgt
    46561 catataaaat cccccttttt tctcatcata ccttgatttc caaaaggagg ataacgtttt
    46621 gcttgtgaca gaaaaaacgg aaactattat atacgtgata cggcttctga taaatgccaa
    46681 aacgaacgta cttgatcaaa aacggttaaa tcaggaatca catattcagc agaaggaaga
    46741 tttatgtttt cacctattcc caatacatgc atgttaccag ccaaagctgc ttgacaacca
    46801 gcttctgaat cttcaatgac caagcatgca tttggcggta cattcaattg ctgtgctcct
    46861 ttgagaaaaa cttctggatc aggttttgct tttgatactt gcgtgccgtc aataaggaca
    46921 tcaaaataac ttgtcaaatt cgttttttct aaaatgaggc gtgcattttt gcttgctgat
    46981 cccaatccga tcttgatgtt tgtttttttc aagtactcaa gaacagctat tgcaccaggc
    47041 aaaacggatg tttcatccat tttttgaatc gcttgaagat atagattgtt tttttgctca
    47101 gcaaaggctg atttctcttt ttctgagaat ttctgttcca tttttccaaa tgacagcaat
    47161 cgctctagtg actcggttct ggatattcct ttcagttgct cgttttcttt ctcagtgaat
    47221 ggaatggaca gctggttagc taaaaccagc cacgcttcgt aatgataacg agctgtgtct
    47281 acaagaacgc catctaaatc gaatagtgct gctttgattt tcatcgatta cctccttgaa
    47341 aatgaacgac ttgaagtaaa gatgcgatgt ttttagctgt gcgatgaaga ttatcagtgg
    47401 cttgctgtaa agcttcttgt aaaggcacag ctgcaggaag gatcggaaaa acggccgaaa
    47461 tgccaacatc tgctgctttt tcgcttcctt taccaacact tccgcaaata gctatcacag
    47521 gaacgccctc tggtactctt ctagcaatcc caactggcgc ttttcctttt agactttgtc
    47581 catctagacg tccttcgcct acgattacga ggtctgcttg ctgtacccgt atcgaactcc
    47641 agtaaatcca atacgtagtc gatgccagag actattttcc ctgaagcaaa tgctaacagt
    47701 cctgcaccca ttccaccacc agcactactg cccgatgcct cgattacttg aggggcaaat
    47761 ctcatataaa aatcatgcat ggcttggtca gccactgcta attcattttc tttcaagcct
    47821 ttttgtggtc cgaaaacaaa agaagcgcct tcttttccgc ataaaagatt agtaacatca
    47881 gtaatgacag tgattttggc tttaatcagc tcaataggca cgttcgtttt atcaactgta
    47941 gcaattttac ctagattttc tcccagtgct tctacttttt gattatcttg atcgtaaaaa
    48001 gcataaccta gaccttctgc catgccgatg ccgccatcgt tggtactact tccgccaacg
    48061 ccgatgatga tgtgtcgaac accttgttgc aacaattgtc gtatcagttc tcctactcct
    48121 cgcgtggata acctaagtgg ctgttgttgg tttagtggga catctttcaa tccgacgatt
    48181 tctgccattt caaaaatcgc agtttgaccg tctgaagcgt actggatttc tttgtttttg
    48241 ccaaatgcat tagtaacgac tgctgtttgc cgcttcaacc ttaaactgtc tgctaatgat
    48301 gaaaccgttc cttctcctcc atcgcctata ggtagcagat catattcagc ctgaacaaaa
    48361 acttcttgaa aacctgattg aatggcttgt gctgcctcta aggctgtcag actttctttg
    48421 aaagaatctg gcgcaatcag aatacgcatt attttcccct ccgttccaag atcagttctt
    48481 tttccagctg gtattggtct ccattgacaa aaataggtaa tgtttctttc gtctcttttt
    48541 ttattactat ttgttttttt gtcaaaacaa atagcaacct gtgatttttc caaataaaag
    48601 gaaaagaaat tttttcccat gctgaaggaa gtcttggaga aatagaaaga gttccattcg
    48661 ttactgtaat acctgaaaaa ccaaatataa ctgctagcca tagtgctcct aatgaagcag
    48721 catgaattcc gtcatctgat gaatgcggat ctggtcctaa atcaatcagg cacgcttctt
    48781 ggaataaatg atacgcccat tccatttctt ggatacggca agcctctatg gcgtgaatcg
    48841 ctttactcaa cgatgagtcg tggattgttt tgttttcata gtaatgcaaa ttcttagaaa
    48901 cgattggttg agaaaaatac tcaggtaata agtataacag catgacaaga tctgcttgct
    48961 tgctgacttg gagttcattt acttcgctac gggcataatc aagtaaaata gactggctgc
    49021 caggacgttc tttgtaagtg gtcaaatcaa taactggctt actgagatac gtgtcatctt
    49081 gcggaatcag ctgttctttg ttaggcactg gcaaatataa cttatctaaa aaattttgac
    49141 attctttcaa aaaaagcgga tcgccagctt gatatttaac agctaaggcc acattataat
    49201 gcgccagata gttcgtatat gcattattgt cgatatattc tgtgtattca tctggaccaa
    49261 tgactctgtg tatctctaaa tgatcatcga cttttacagc tctgctcagc caaaaagctg
    49321 ctgtttcatg aatgagcgtt tttccctttg tttctaaaaa cgattgatcc tctgttgctt
    49381 ggacatactc ttgaacggca taagcaaggt ctgcactgat atgatgttct gcggcagctg
    49441 aagcaacttc ttgtcttgtt ccggttttga tattcaatgc agcaaatttg ggtgtctctt
    49501 cctgaccagt gtgtgcgctt tcccatggga aaagtgcacc ttgatagtga taatctcttg
    49561 ctttttgccg tgcgccttct aatcgatcat aacggtacag cagcaaattc tttgctactt
    49621 caggaaactg atataaaaag aaaggcagga taaaaatttc cgtatcccag aaaacatgcc
    49681 ctttataacc ttcgccagtt agtcccttcg cccctacact aacacttgga tcattgcggg
    49741 gagtcataat ctgtaaatgg taagcagcga agtccatcgc taactgatcg aaagaagaat
    49801 cgccttggat cgttatccga tgctgatgcc aaaaatctgt ccaataagac atcgactgct
    49861 ctaaaatttc tgtataacta acgcttttct gggcttgggc aatacttttc ttaaacgggt
    49921 ctgtttgctg aagagaggta gagagcacga tgatcttctc cagcgtaaag gacgtatttt
    49981 ttctgatctt ttctgaaaag gaagcggtga tccggcgatt ttttgcttgg aatacacctg
    50041 ctttattgaa tttagctgca atcccaatat caattttact ttctgttgtc tggtaggatg
    50101 cactgatcaa atcgtcacca aatactcgca gttctttttc aaccaactgc tgtgtaccaa
    50161 aattggtttg ctgtccatcg atacccgttt tcacatctac ttggcagtct tcggtcaacg
    50221 gcttgatgac cactttcagg gctgcctgat ggagattatt ttgatcagca acacgacaag
    50281 taatgacttc cacttttttt ccttgagcag tttcaatgag cgcatagcgt ttaagctctc
    50341 ccatttctaa atctagtgcc cgctcgtatg caagaacttt catcgtctca agagaaaaat
    50401 gttcaccgtc gatttttatt tcccatctga tgacatctgg gagattcact aactcggctg
    50461 attctgctcc taaaggtcgg ttatatacac cagctgcaaa cattcccctt acttgattcc
    50521 gatagtcttc ctcctggctt cctctgatac caaaaaatcc atttccaatg gtcatctgac
    50581 tcgcatactt attgatatac tctaaatcaa actgtttttc ttctactttc ataaggtaat
    50641 aacttctcct gtttctgccg aacgatataa attttccacc agattctgga tgacatatcc
    50701 ttgccagcca tctgcaatca tctgagtctg gtcaccgagc gcccgattca caaaatgagc
    50761 catacttagt tcatgtgggt cattaactgg cgtggtcttt tgtaaggaaa ccagctttcc
    50821 atctacatct tcgaatagtt cgcctggata cagattagca cctgcctgat cgccacaaaa
    50881 agccacattc agtttgtctt ctctgatatt caaagcaaac gaagtattca gttggatgat
    50941 cttgccgttt tctaattcca gtgtgccaaa aattgcatct tctactgtga atttgcttgg
    51001 atcccatttc ccgaatttcc ctgaattttt tttcgtacca attttttgga agctgtacgc
    51061 ccgaacgctt tttactttag gaaatccagt aatatacatc gctgtatcaa gcatgtgaat
    51121 gccataatcg atcaaaggcc cgcctccttg aagttctttg tcgataaacg ttccccagcc
    51181 tggaacacca gaccgacgaa gcgcattcgc ttctacgtaa taaatatccc cgagaacatc
    51241 aagatgattc ttgatcattg ttgcttcgtc agaaaaacga tgctggaatc cataagccaa
    51301 aatctgcccg tttttatcgg ctgtctctgc catctttctt gcttgatcag ctgtcatagc
    51361 tggtggtttc tcgcacatca ctgaagcatt agcttcgagg gcagccaata catgttcgaa
    51421 gtgaaagcga ttggctgtac agacactcac gatatcggga gaaatctcat tcagcatttg
    51481 tgtaactgat gtatacgcat gagaaaatcc tgtacgtcgg caaaagtctt ctgctcgctg
    51541 gctgactgga tcaaccacag cggccaattc taagacatct gatctttttt gataataagc
    51601 agcatgtact ttttctgcta cttgtccagc gcctatgatt gctactttct tcatttattt
    51661 ctctctcctt tgcttattta cttattgtta ttctttctct attctttctc taaatcattt
    51721 aaatgacgcg ctgtttccag acagtttttc aagtattgca atgattcctg ataaattttc
    51781 acttcatttt ctcctcgcaa acgacattca tataccatat aaccttcata ctcattttga
    51841 agtagcgttt gaaaatggcg agtaaaatca attgcgccag atcctggttg gtagcgatga
    51901 ttatctgcta aatggatatg acccacatac tctttttgcg tagtcaatgc ttttgaaata
    51961 tcatcttctt caatattcat atgataaaaa tcagcgatga tctgcacatg tttcaattga
    52021 ttagagtcaa tgtaatgacg cgcatccgct aacaaattga tcatatggtc ttggtatcgg
    52081 ttcaaaggct ccaaataaat aaccgtacct gtttcttcgg ctactttatc taaataacga
    52141 agtgattcgc tgactgcttt atagtctcct tctttgcttc ggggtgaaac catcggcggt
    52201 aatcgataag tgaacattcc ccaagctgct gggataacga ttcctttgcc tccaacctcg
    52261 cttagtgcac gcaaaatctc agcaatttga cgtacaccat taagacgccg ttcttcgata
    52321 aagtcaccga tccacccatc gtagccgtta caagctgtca ctactgggat ccctgtttct
    52381 ttgattgcct gtttgactgt gtcgatattt tctattaaca acttaccgtc gatttcgtat
    52441 tcttcaaatc cacagtcttt gataaatcta aatttttcta aaatgtctct tgggaaaaat
    52501 gcttgatctt gcgtaccgaa tttcattgtt ctcctcctcg gacttgaacg cccattttga
    52561 tactttcttc tgggtgctgg tctacataac gcatgtaagc ttctgcgctt tctgcaaatg
    52621 gcacaactgg atcaatgatt tcttcacaat ccaagtaacc gttcatcaat aattcccaac
    52681 atgtgtcctc gattcgttta cggttccatc ttgggtaatc tgggtttggt tcactagatg
    52741 ctcgagcaaa gacgattttt gcattattga aatgtgcttc gcgaccaaga ttaaacccag
    52801 ctggaaaagg tttagcaaag gcaacataag aaatgatccc gccatagccg atccctctta
    52861 aagaagcttg taatgcggca gcactgccac tcgtttcaat aactgaatct actccacgtt
    52921 tatctgtcaa ttcctttaga cgaaaaccga catctgcttt cataggatct agcgtgtaat
    52981 cggcaccgta tttttctgct aattctctac gctgcgctat gggatcgatc ccaacgatgg
    53041 tagtagcacc tgctttttta gctaattgaa tcgcaatttg cccgatagcg cctaaaccga
    53101 agattgctac agcatcacct gctcgaacat gtgcttcccg gataccacct aaagcgaatt
    53161 gagcaggatc gtaacatact gcatttttcc attgatccaa ccgatcaagt ttacgtagtt
    53221 tataattatc aacaccgttt gtaatcagcg tttcacgaat gggtccataa gtgcagacta
    53281 actctccttt ttgatactct gtcaccaaac tgcccgtttc ttcgatcgta ccgacaatca
    53341 tatttcctaa ttgaaattcg ccaaatacca caccgcgttg gcttccttct tctcttggca
    53401 agaataggcg ccattcttct gaaaattctt catcgataaa tggacttaat cctctaaagt
    53461 cgacaatttc tgttcctggt tttggagtag cgaaaattac tcggatacgt acttcgttct
    53521 tttcgatctc acggtcttcg taatctttga atccggctat tcttggttct aatgcaacta
    53581 attttttcat cttttctttc ctctttcttt tctttttatc cttttacgcc gccttcgact
    53641 aaatcacctt taataaatcg ttcagataac gcatacataa tgatgactgg taaagcggtc
    53701 accaatgaag cggccatcat tcgtccccaa atgtagtctg gtgtactgaa taatgtgttc
    53761 aaaccgattg gtaaagtaaa tttttcggag gtcgacagga aaatcgaagc aaataaataa
    53821 tcattccacg aaaccataaa gcaataaacg aagacagaaa tgattccaga aatagccaat
    53881 ggtataatga tccgaataat gatctggatc cggctaagac catcgatcat tgctgcttct
    53941 tcaatatcag tcggaatcgt gtcaaaataa ctttttagca tgaatatcgc agtaggaagt
    54001 gtttgaacga ccatcgtgat aatcagtgct gtattggtat catacaaacc tagaccggaa
    54061 atgattttaa ataatggaac aacgatcaaa ataccggaaa acatataaac ggtgtaaaag
    54121 ctagcattga ttttcgagcg agctttgaag cgtagttttg acaaagcgta tgccccgctg
    54181 atcccaatca ctactgcagt tacggaggct atcaaggaga tcactaaact atttttgaaa
    54241 tacatgatga atggaaaaat ctcagcattg aaaatatcct gataatgttc aaaagtccat
    54301 tctttcggaa aaatagtttg tgtcgttgca attgcttctt gtgaatcctt gaatgatgtc
    54361 atcagcatga tcaaaaatgg aaacaaattg acagctaata gcaacaaaat cgtcgcgtaa
    54421 aataagattt tcctgctctt tttatttttc atttcaagtc caccttcttt cgtgagagca
    54481 agacgaccgc tgcaataatg atgaacaata gaatcgatat agctgctgct tttccaagat
    54541 cattgaacgc aaaggctgtc tgatacaaat aaacgcctaa gatattgact ttattcgtca
    54601 gtagatagac atctgtaaac atatagaaca tccagataga tcgtaaaaca atcacagttg
    54661 ctagtactgg tttgattgcc ggcagtgtca cgatcttgaa cttctgccaa gcatttgcac
    54721 cgtccatttc agctgcttca taaagcgttt tgtcgactgt ttgcaaaata gccaaaaatg
    54781 aaataaacgc atacggatag tatctccaga tagcaaacaa aacgaccata aagaagctag
    54841 caactggctg gtcgaaccac agcggtgctt cgttgaaaag ctgcaaccta tctactaaca
    54901 gatcattgac gattccataa ctattattga acatatattt ccaagcaaaa atcaaagaaa
    54961 tagatggcgt aacataactt aataaaatca gtgaacgcac cgttttccta aatctgaact
    55021 tgcggttaag caaaatagct acggctaatc ctaaagctgt actgcctgct acaactaaga
    55081 atgtgtaagc aactgttaaa cctaaagaac gataaaattc actatcactt aaaatttcaa
    55141 gataattgct taatccgata aaaacggcgt ctgagttagg attcagcggc atatcgaaaa
    55201 aactgatctg aatattggaa agcattggaa tcagcactaa aacagctaat aaaagcatgc
    55261 ttggtgctaa aagtcccatg gctaatttta agtcagaatg tttccttgga cgattcattg
    55321 aaacctcccc ctgataatct gccaatagct atctactttt ttcgaacacc attggcagaa
    55381 cttcttgtta tttcgtattt ttttgtgcgt cagctaaagc ttgatcgaca tcttcgtttc
    55441 cgacagttac ttggttcact gcttttccga tcacacctga gcttgtgata tctcccattt
    55501 tcgtgaaatt cttttccccg actagtccaa agacttgtac ttcatcaaaa gcactagcaa
    55561 tctcagatgg cagctcgcca aaagacttga tgacttcatt ttctttgtaa ccatccagtt
    55621 cgactacttg tttattcact ggttgagcac caccaggaga catcaagacc cacttttcca
    55681 tgtttttcgg ttgagataag aaagctgtga atgcttcagc agcctctttt tgtttctcgt
    55741 ctagaccaga agaaatcgtc aatccagata ctgtgccata gacagcttga tctttttgtg
    55801 ttgggatagc aaaaccgata ttttctgagt tccccgcttc ataaacagaa ggcaaaatgt
    55861 aagttgaata gattgccatt ggtgctgttc cattcatgaa agcatctttg atttctgtca
    55921 catcattaga gcctggcatc gtgtattggg ataagttttg ataaaacgac aatgcttctc
    55981 tcatcttttc cgtatcgatc gttacctcgc cttcatcatc caaaacattt gctccatttg
    56041 acaaagcaaa ttgagagaac gcttgctcac tcattgttcc ttcagcagta ggcatagcaa
    56101 ttccatattg tttattctct ttatctgtga aatgttgagc aatttttaaa atatcatccc
    56161 aattttctgg ttctgaaaaa ccagcttctg ataatttttc tttgtcatac caaatacctt
    56221 gaacccagcc gctaattggt gcagcaatat agctttttcc atcttctgaa cgaacaaggt
    56281 ttttggctcc gtcataataa ttatcttctc cagcttcctc gataacgttt tggactgccg
    56341 tctgatcaat cagttcatct ttatccatca ctttagcaaa atcctgactg acttccatta
    56401 cttctggtaa tttaccggaa cgagctaacg taacgatttt tgtattgaac gctgattctt
    56461 ctaccggcac ttgcttgatt ttgatgtctg gattttcttt ttcaaaatca gcaaccagtt
    56521 cattgatcac acttaatcgt tcctgttcga cagaagagtg catgaattga atcgttgtct
    56581 gttgtgattc gttagagctt tgactttcac cacaagcagt aagaagaaat ccagtagcta
    56641 gcagacaggt agtaacaata atttttttca tttttttcca cctcttattt tattattttc
    56701 agccacatat attcatacgg ctggaatatc aactttcgtt cttgactgca atggataatt
    56761 tgtccagaaa acaggtctag gtaatctcct tttggtaaaa gataacctgc tgattgattg
    56821 gaaaaattat ggataactaa gactttctct tccttcgctt cgcgaataaa actgataaaa
    56881 gaatctgctg agtccagtaa gtaaatcaaa ctattcggat ggaaggcttt ttcttttttt
    56941 cgaatagata gcatctgggt caattcacca tatactgctg ttcttaatgg atctgtctgc
    57001 aactctttct caatatctgc taacaaaaat ttttgccgat tgatttttcg attctctcct
    57061 gttttttcta cgcctttgta atcatttcga cttcccagca cactttgaat ataaattgcc
    57121 ggcacgcctg gtaacgttac caaaaatgaa tgagcagcta aaaagcgttt gcttctccat
    57181 tccgtcgtat ctgttggaga acttaacgca tctagatacg tagcgtttat ctcatatgga
    57241 ctggtcgttc catctgggtt tttcttgtag tttactcggg caccctcttt cttcagctgt
    57301 tccactaatt caaggatcgt tttttccggc agaatacctc gtaccggatt caatccgata
    57361 ccgtcatggg aggctaaaaa attaaaaaaa gttgtttttt tcgatgcgaa atcgatggtt
    57421 tttaaccatt ctttcaactt acttgtatta cccgaacgaa tcgcttgaag ggtcaaaggt
    57481 ggtagcgaaa actgatagac catctgtgct tcattctcgc cattcccaaa ataactgata
    57541 ttatctttgt gtggcacatt tgtctcagtg atcaataccg ttcctggagc tgcttcatca
    57601 gcgattgcac ggaataattg gatgatttta tgcgtttctg gcaggtgtat acaagaagtt
    57661 cctagttctt tccataaaaa tcccacagcg tccaagcgaa taaatgaagc gcctttttct
    57721 aagtaaaaca aaagaatatc gatcattttc aataaaactt ctggattttt aaaattcaag
    57781 tctacttgat cttcgctaaa agtcgtccat acgtgttttt ctttcccgtt tttgtcaatg
    57841 aacgttgtaa gtaacgggct ggtccttggt ctagtcacgc tgcttgtatc tgtatccagt
    57901 ggcaaatcaa taaaaaaatc tttgtacgta tcattcaagt ttaaatattc ctgaaaccaa
    57961 gtactttcgg cagacatatg attgcagaca aagtcgaaca ttaaccgtgt ttcgcttctc
    58021 atttcttcta tatcttccca tgtgcccaaa cggtcgttga ttgcctgata atttacaacg
    58081 gagaaaccat catcagatgt atatgggaaa aatggcaaga aatgtacaac tctaaatgta
    58141 tctgccaaat acttttgata aaatttctta aatgtacgca aagttggttg ctgctcttca
    58201 aaaaattgat ccccataagt aatcaagaaa atatcttctt cgtcccaatg cagtttgcct
    58261 gcctggcctt tcttctgctg ataaacactg actttttgtt caatcataga ggcggttttc
    58321 tcagacaaat ctgagtcgcg ataaatcgct tgtaaaagtt ttgtatacat cggattcccc
    58381 tttctttttt gggagcgttc ccaacgattt gatcatatta tggaaaacgc ttactgtcaa
    58441 tagcattttt ttaaataatt ttgatataca caaaaataca aataagaaag cttatgtatc
    58501 aataattaat agcttcttaa aaaaatatgg tctctcatga tcatgcaagc tattcctaaa
    58561 ttttcaaaat catagacaaa aaagaatgtc tattgtacta tggaattatt ctaaaacgct
    58621 cccaaaaagg aggtgcatat aaaattgaaa cctactattt atgacattgc gaaaaaagct
    58681 aatgtctcaa aatcaactgt ctccagagta ttaaataacc agccaaatat ctctgataaa
    58741 gcaaaagcaa gtgtacttca agcaatcaaa gaattgaatt atgaaccgag caaaattgca
    58801 agaggactgt caggaaatgg ttttgacgcg attctagcat tatctcatcg tacttctcat
    58861 acgacctatg gcaatccttt tttttcagat gttattcaat ccatttctgc cgtttctgaa
    58921 caaaataact tcgacttgat tctacaaacg gcaaaaacac cagaagaaga gctagaaaaa
    58981 tgcgcgaaaa agatccaaga aaaaatgatc cgaggaatta ttattttaag ctctacgatg
    59041 gatgaaacac ttctttttga attggatcaa tacaatattc ctattgtggt aatcggaaaa
    59101 gtcggaaacg actacacgaa catctactca gtggacacgg ataatttcca ggatagttac
    59161 gatttggtgg aaacactgat caagaatgga caccgtcata tcggctgtct ccatgctccg
    59221 acagaagttt atgtatccaa tgatcgtctc aatggttacc gtcaatgctt atttgatcac
    59281 cacctagata tccgtaatga atggattata gacggcggct ttcgcttaga agatagcatg
    59341 gcagcaatga atgatttaat gaagctacga gaaagaccaa ctgcgatttt ttctaccgat
    59401 gcattaaaat cattgagtat ttacaaagtg gcagaacaat ctggaaagca gatacctgat
    59461 gatctttcga tcgcaggatt cagtgatccc ttattgtcct ccctattttc gccacagttg
    59521 actcggatag acattccgac aaatcagtta ggaatcaaag ccacagaact tttattccaa
    59581 ctaatccacc accaataccc tgaagaaaaa agaatcattc tttcaaccga gattactctc
    59641 acagattcga tccaacaaca agaaatgtag aacagaatta taaaagaaca gaaacactga
    59701 ggataagtca gttactctgt tcttttttca atcactcatc cctcgttacc ccctcccggt
    59761 attttagcga atttttctta ttttccatct gttttagata gctgtggata actataatgt
    59821 ttgtgttaaa ctattttttc cacagggaag aaaaaactat ttttaaccac gttgtgtata
    59881 agtttttata gttcgttttt ttaaacacat ttacccttat ttcaatccat gttatttggg
    59941 gatacttatc tctaaaacat aaatagaata acagacatgt ctactggttt tgttttattc
    60001 gaggaattat tcaataatag ctagcaaaat attaaaaata accgctctat cttcccatca
    60061 atttattttg cattcaaaaa atgatctatc tttacttttg ttgattgact tctcttggaa
    60121 gatgatcgtt ccgatacctt aattggatac gaaccgatgt tgttaagatc ctttcccact
    60181 tttcttctag ttagacaaat tattttagac aaaccaaaaa agaggttgtg acaataattt
    60241 gtcacaacct cttttttaaa atatctattt tgtttgattg ttcattgcgt cttctgcagc
    60301 catttgcgct tcaatatctg aaatactgta gacattttcg cttgctacac cgacttcttt
    60361 tggttccatg tcacgataac gttgcatacc agtaccagct gggatgatct tacctataat
    60421 aacattttct ttcaaaccaa gaagaggatc tttcttaccg cggatcgcag catccgtcaa
    60481 gacacgagtt gtttcttgga atgatgcagc agataagaaa ctatttgttt ctaatgatgc
    60541 ttttgtgata cctagtaaga caggtcgaga agttgcagga acgcctcctg agactagcgt
    60601 attgtagttt tgatctttaa agtctgcgat gtctaataat gtacctggta agatatctgt
    60661 atcacctgga tccattacac gaactttacg cagcatttga cgaaccatta cttcgatatg
    60721 cttgtcgccg atttctaccc cttgcatacg gtaaacacgt tgtacttcac gaagcaagta
    60781 gttttcaaca gataatacat cgcgaacttg caacaattgt tttggatcga ttgatccttc
    60841 tgtcaatggt gcaccacggt ggatcatatc gccttctgct actttcatac gtgctgtata
    60901 aggaacagta tacgtacgtg tatctgtttt tcctttgatt gttacttctt tggtacgtgt
    60961 tgcaggatct tctgagatat cgatgacatc ccctgttact tctgtgatca ctgcttgccc
    61021 tttcggatta cgtgcttcaa agatttcttg gacacgagga agaccttgag tgatatcgtc
    61081 tccggcaacc ccacctgtat ggaatgtacg catcgtcaac tgagtaccag gttcaccgat
    61141 tgattgagcg gcgatcgtac caactgcttc tccgacttca acgtcagagc cagttgcaag
    61201 gttgcgtccg taacaatgtt tgcagacacc atgttttgta ttacatgtga agacagaacg
    61261 gattgtcact gtttcgacac ctgcatcaac gatttgtttt gcgatgtctt cggaaatcaa
    61321 ttggtttcct gcaatgatca attcatttgt ttcaggatga cgtacttctt tacgggtgta
    61381 acgtcccact aaacgatctt ctaatgattc gatgatttca tttccctcgc ggatggcttg
    61441 gatttcaaga cctcggtcag taccgcaatc ttcttcacgg atgattacat cttgggccac
    61501 gtcaaccaga cgacgagtca agtaacctga atcggcagtc ttcaaggctg tatcggtcat
    61561 ccctttacga gccccgtgag ttgagataaa catttccaag acggatagtc cttcacggaa
    61621 gttcgaaatg attggcaatt ccatgattcg accattagga gcggccatca aaccacgcat
    61681 tccggcaagc tgtgtaaagt tggaaatgtt accacgagct ccagaatcag acatcatgaa
    61741 gattggattt ttcgcttcca aaccttccat cagtttttgt tggatgctgt cttttgcagc
    61801 attccaaaca gcaatgacac gttcataacg ttcgtcatct gtgatcagac cacgacggaa
    61861 ttgttttgta atagtttcaa cttgtttatg ggcattttca atgatttctt gtttttcatt
    61921 cagtacactg atgtcagcga tccctactgt gataccggca tgtgtcgaat gtttgtaccc
    61981 taagtctttc atacggtcaa gcatcttaga agtttctttt actttgaatc gtttgaagac
    62041 ttctgcgatg atgtttccta agtttttctt cttgaacggt aatactaatt cttgctcttt
    62101 gatatgtgca ggaatatcag taccagcttc aacaaagtat ttgtccggtg tttggacggt
    62161 caagttgaag tctgttggtt cattcagata agggaattca ggaggcatga tctcgttgaa
    62221 gatgatctta ccaactgttg tgatcatcgt acgttctttt tgccattcag taaatggttt
    62281 ttcacctaat gagttagggt tcacaccgat acgtgaatgt aaatgaacat atccgttgcg
    62341 ccatgcgata acggcttcgt tttgatctct gaagaccatt ccttcgcctt cgcgtccttc
    62401 ttcttccatt gtcaagtagt agttacctag aaccatatct tgagaaggag taacaactgg
    62461 tttaccatct ttagggttca agatgttttg agcagctaac atcaacatac gtgcttctgc
    62521 ttgtgcttct tcattcaatg gaacgtggac agccatttgg tctccatcga aatcggcatt
    62581 ataagcttca catacaagtg gatgcaaacg gattgcgcgt ccttgtacta aaactggttc
    62641 gaatgcttgg ataccaagtc ggtgaagcgt aggtgcccgg ttcagcaata ctgggtgttc
    62701 cttgattacg tcttctaaaa catcccatac ttcgtcttct tgacgttcga ttttacgttt
    62761 cgcatttttg atgtttgaag ctagttcgcg ttgaacaagc tcacgcatca cgaatggttt
    62821 gaataattca atggccattt cttttggaag tccacattgg tacatcttca agaaaggtcc
    62881 aacaacgata acggaacgac ctgagtagtc gacacgttta cctagcaagt tttgacggaa
    62941 acgaccttgt ttccctttca acatatgaga aagagatttc aatgggcggt tacctggtcc
    63001 agtaactgga cgtccgcgac gaccgttatc gatcaatgca tctactgctt cttgcagcat
    63061 acgtttttca ttttgaacga tgattccagg tgcattcaag tctaacaaac gtttcaaacg
    63121 gttattacgg ttgattacac gacggtaaag atcattcaag tcacttgttg caaaacgtcc
    63181 accttctaat tgaaccattg ggcgtaaatc tggtggaata acagggataa cgtccataac
    63241 catccagctt ggttgatttc ctgaagcgcg gaatgcttct aaaatatcca aacggcggat
    63301 tgcacgtgta cgtttttgtc cttgtgcaga tttcaattct tctttcaatt cggctgcttc
    63361 tgcatctaga tcaacattat caagtaattg tttaatcgct tctgcaccca tagcagcttg
    63421 gaattcttgt ccatattgtt cacgtttttc gcgatattcg cgttctgtca acaattgttt
    63481 tttctctaaa ttcgtatttc ctggttcgat gactacatat gaagcaaaat aaatgatttc
    63541 ttccaaagcg cgtgggctca tgtctaatac aagacccata cgtgaaggaa tccctttaaa
    63601 gtaccagata tgtgaaacag gagctgctaa ttcaatatgt cccatgcgtt cacggcgaac
    63661 tttcgaacga gtaacttcca ctccgcatcg atcacaaacg attcctttat aacggatacg
    63721 tttgtatttt ccgcaggcac attcccagtc ttttgtagga ccaaaaatcc gctcacagaa
    63781 caacccttcg cgttcaggtt ttaaggtacg atagttaatc gtctcaggtt tttttacttc
    63841 accgtaagac cagcttctga ttttttcggg agaagccaaa cctatttgca tactttcgaa
    63901 tttatttaca tcgatcaaaa ggggttccct ccatttcatt taatggcaaa ccgcaagacg
    63961 gctgatcttc gaccgccttg cagctcatag accggattag tctaaattat cttctgcggt
    64021 ttcaaagttt tggatgacat cgtctttttc atcttccact tgttcagcag ctttcttttc
    64081 taattctttg gcagtttgct gttcggcaaa ttttgtcaaa gcatcaacag tgatcaaatc
    64141 gtcgtcttca tcgtccatgt cgcgaagttc gatctctgtt tcttcgatat ccaagacacg
    64201 catgtccaga cctagtgatt gcaattcttt caccaatacg cggaatgatt ccggtacacc
    64261 tggttttgga attggttcac ccttgacgat tgcttcgtat gttttcacac gtccgactac
    64321 gtcatctgat ttgtatgtca agatttcttg taaagtataa gccgcaccgt aagcttccag
    64381 tgcccatact tccatttcac caaaacgttg tccaccaaat tgagctttac ctcccaatgg
    64441 ttgttgtgta accaatgagt aaggtccgat tgaacgtgca tgcaatttgt catcgaccat
    64501 gtgagcaagt ttgatcatgt acatcacacc gacagacaca cgtccatcaa atggttcacc
    64561 tgtacgtccg tcgtacagaa cagttttcgc atcgctggcc ataccggctt cacgaactgt
    64621 ttcccatacg tcttcatcac ttgcaccatc aaatactggc gttgctacgt ggatacctaa
    64681 ctgacgtgca gccataccca agtgtaattc caatacttgc ccgatgttca tacgtgaagg
    64741 cacccctaaa ggattcagca tgatatcgat tggtgttcca tcaggtagga atggcatatc
    64801 ttcttccggc ataatacggg atacaacccc tttatttccg tgacgaccgg ccattttatc
    64861 cccttcatgg attttacgtt tttgaacgat ataaacacga actaacatgt ttacgcctgg
    64921 tgacaattca tctccagctt cacgagtaaa gattttcaca tcgtgaacga taccgccgcc
    64981 gccatgtggt acacgtaatg atgtatcgcg gacttcacga gctttttcgc caaagatcgc
    65041 atgcaataga cgttcttctg cagataattc tgttacccct tttggcgtga ctttccctac
    65101 aagcaaatcg ccatcttgga cttctgcacc aatacggatg atacccattt cgtctaaatc
    65161 ttttaatgcg tcttccccga cattagggat ttcgcgtgtg atttcttcag gtcctaattt
    65221 tgtatcgcgg gcttctgatt catattcttc aatatggata gaagtgtata cgtcatcttt
    65281 taccaaacga cggctcatga tgatcgcatc ttcatagttg tacccttccc aagtcatgaa
    65341 accaactaaa acgttttggc ctagagccat ttcgccttgt tccatagatg gtccgtctgc
    65401 cagagtatcc cctttttcga ctttctcacc caaatgaacg attggacgtt ggttgtagct
    65461 tgttccggag tttgaacgac ggaattttgt tacatcgtat ttatctaatg caccgttgtc
    65521 gcggcgtacg cggatttcag aagcatcaac gtattcaacg acaccatcat gtttacataa
    65581 caaagcagct cctgagtcat gcgctgattt gtattccata ccagtaccaa cccatggtgc
    65641 ttgaggattg atcaacggca ctgcttgacg ctgcatgttg gcacccatca aggcacggtt
    65701 ggagtcatcg ttttccaaga aaggaataca tgctgtcgca acggcaacta cttgtttagg
    65761 tgatacgtcc atgtagtcta ctttatcaat agatacttct aagttttcac tttgagcacg
    65821 agccataaca acgtcttgag caaatgttcc atcttcattc aatgggctgt ttgcttgggc
    65881 tacgatgtaa tggtcttcga tatcagctgt caagtagtcg atttggtcag taacacgacc
    65941 tgtttcacgg tctacacgac gataaggtgt ttcgataaat ccgaatttat ttactttcgc
    66001 ataactggac aaactattga tcaacccgat gtttgggcct tcaggagttt cgatcggaca
    66061 catacggcca tagtgggagt agtgaacgtc acgaacttca taaccggcgc ggtcacgagt
    66121 caatccgcca ggtcctaagg cgaaaagacg acgtttgtgt gtcaattcgc caagagggtt
    66181 tgtttgatcc atgaactgtg acaactgaga agaaccaaag aattctttga tacttgccac
    66241 aactggacgg atattgatca attgttgagg tgtcaatgtt tcagtatctt ggatcgacat
    66301 tctttcacga accacacgtt ccatacgtgc tagcccgata cggaattggt tttgtaaaag
    66361 ttcaccgact gaacggatac gacgatttcc taagtggtcg atatcatcga cattgccgat
    66421 accttccatc aagttcaaga aatagctcat tgaagcaatg atgtcggctg gacgaaccgt
    66481 ttttacagct gcttctggat agccgtttcc gatcacgttt acttcacgtt cagggtctct
    66541 tggtgaaaat actttgatga cttggacagt cattggatct gtgaccacac cgtcttctga
    66601 tggatagtaa gtcactgaat ttaggccatt atcaatgaaa ggagctagtg tttccatgac
    66661 ttggtgagtc agaactgttc ctttttcaac gatgatctcg cctgtttctg gatcaactaa
    66721 tgtttcagct aatgtcagat tcaatagacg tgttttcaaa tctaattttt tgtttacttt
    66781 gtaacgacca acgtttgcta agtcgtaacg ttttggatcg aagaaacgag cattcagcaa
    66841 gttacgtgag ctgtctgctg tttttggttc gcctggacgc aaacgttcgt aaacgtcttt
    66901 caaaccttct tctgttcttg agtcgcttgc gtttttatgc aagtcttttt cgactgtatt
    66961 gcgtaatgtt tcgctgtcgc cgaagatttc aaagatcgta tcatcggaac caaatcctaa
    67021 cgcacgaact aatacggtta aaggaatctt acgtgtacgg tcgatacgta cataagagat
    67081 gtcttttgca tcagtttcca tttctagcca agctccacgg ttagggataa cagtagaacc
    67141 gaatccttct tttccgtttt tgtcgacttt tccatggaaa tagacacctg gtgaacggac
    67201 taactgagaa acgattactc gctctgcccc gttgatgatg aatgttcctt gttctgtcat
    67261 taatgggaaa tcaccgaaga atacttcttg tgctttgatt tcgccagtct cgcggtttgt
    67321 caaacgcaat gttacatgta atggtgcaga gtagtttgca tcatgcgcac gagcttctgc
    67381 tactgtatac tttggttctt tcaattcata atctacaaat tccaaagata gattgccatt
    67441 gaagtcatcg attggcaaaa tgtcttcaaa catttctcta agtccctcat ctaagaacca
    67501 ttggtaagag tctgtttgaa tttcgatcaa atttggtaat tccaacactt cactgattct
    67561 tgcgaaactt ctacgttcgc gatgttttcc gtattttact acgtgtccag ccaagctctt
    67621 cacccctcta aaatgtcatt caaaaagact acccgatcaa ttgttacaat aatatttcaa
    67681 atattatttt taggtaacaa cagacatttt ttgcggcttt taggcaaaaa aaaaccaaaa
    67741 atagaacatt ctcttgaaac cttacttcgt gagcttctac ttttgtttcc ttatattaaa
    67801 gccgaaacgg acaatatttc gtttctgcct tttcttcatc agatagtttt tgcaacttca
    67861 tatactacac ttattttcta taaaagtcaa cacttcctgg cgtttcttct gctttttcgc
    67921 aacattttct taatgttttc aattttatgt tttcaattat ctgacaattc ttaattgaca
    67981 gaccttttta tctcggttat actttagaaa attcattttg tggaggaact tctatgcgaa
    68041 ccaacatcaa ccaaatcgca ccaacacaac tgaataactt ttactttagc tactttagat
    68101 aggtttgtat tcatcatgat ggatacaaac gttaaacgac agtttgtatc tatgatggct
    68161 aaagaacttt gtattgcagc aaaaagccac gtagacaaaa tgctaagtgg ctgagcagtg
    68221 aaaatacaaa gtaaaagtat caagcattct actttttcaa tttattttcc gacttttcaa
    68281 tccatttgga cattttgtct gaatggattt tttgtttccc aaaattaagg aggaaaaaga
    68341 aatgaaaaaa aatataatca tcggattagg cgctgccctg ctactagctt taggaggttg
    68401 cgcacaaaaa gacaattccg cagaggcatc agatggacaa aaaacaattg gcgtattgca
    68461 acttgtacaa cataactcgt tagatgctgc ctataaagga ttcaaagaag gattagcaga
    68521 agaagggtat aaagaaggcg ataatctaac aatcgattat caaaatgccc aaaacaatca
    68581 agacaacctg aaaagtatga gtgagaaact cgtcaaagaa gatcctgatt tgctacttgg
    68641 aatcgccact ccagcagcac aatctttatt aaatgaaaca accactatcc cgatgactgt
    68701 cacagcggtc acagacttgg aacaagccca tttagtcaca tccaatgaaa aacctggcgg
    68761 caatgtgact ggcacgacag atatggtgcc aatcgaaaaa caaatcggct tactgttaaa
    68821 tatcgtgcca gacgccaaaa caatcggcat catgtataac aacggtgaag caaactctaa
    68881 gatccaagct gatttggcag aaaaagaatt gaaaaaagcg ggcgtcaaag tcaaagtact
    68941 gacagcaaat tccacaaatg atgtccaaca agtgacgaaa agtctagcta aagatgtaga
    69001 tggaatctat attcctactg ataacacgtt cgcaagtgcg gcaagtgttg tcggtgaagt
    69061 cgcaaaagaa acaaaaacac ctgtcgttgc tggcgcagtt gatcaagtca aagacggcgg
    69121 tctagcaaca gtaggaatcg actacgaaag cttgggcaaa caaacaggga tcatggcagc
    69181 aaaaatctta gacggcaaga atccaagtga acttcctgtc gaaacagcga atgatttgga
    69241 actgtatgtc aataaagaca tggccaaagc attaggcatc aatccagaca gcatcaaaga
    69301 agctgaataa aacacaggag gattctcatg acaaatttgc tctctctttt cttatcatct
    69361 gcatcgcaag gggtcttatg ggccctactt gcgattggtg tatacgtcac tttccgtatc
    69421 ttggacattg ccgacttgac tgctgaagga agctttcctt taggtgcagg gatcgcggca
    69481 gtaagtatta caaatggcta tcatccattg acagcttgtc ttctaggctt tttagggagt
    69541 gcggcagcag ggcttgtttc cggactgctt cacacaaaat tgaaaattcc cgcccttctt
    69601 gcagggatca tcaccatgac tggtttatat tctgtcacat cccgtgtgat gggtgcgccg
    69661 aacatctctt tattgggtca gccaacgatc tttcattggg tcgagtcatt cggcataaca
    69721 aaagacaacg ctgtactgat cgtcggtctg ctttttgctt tgatcgttgt atgtttactt
    69781 gttttgttct tacgtacaga aacaggactt gctattcgtg caactggcga taatctggca
    69841 atgagcgaag caaacgggat caacacagat aatatgaaga tcatcggtta tatgatttct
    69901 aatggatgca ttgctctttc tggctccttg cttgcgcaaa acaacggctt tgctgattta
    69961 aattctggta ttggaacgat cgtaattgga cttgcttcta ttatcatcgc agaagttttg
    70021 ttacgtaacc agccactatt tctccgtctc ttgactgtcg ttttaggtgc ggtcatttat
    70081 cgttttattt tagcgttagt cttcgaactg aatgttgaac caagtgattc gaaacttgct
    70141 tctgctttcg tgctagtgat ctgtctttct ttcccgcaga tccagcaaaa attccattta
    70201 cctcaaaaaa agaaaggcgg tagtgtcaaa tgaatgacgt cttaacattg gaaaatctcc
    70261 atcatacctt tgaaaaagga acgatcaacg aaaatcatgt cctgaaaggc attaatcttt
    70321 ccttagatcc cggcgacttc gtgacgatca tcggtggaaa tggggctggt aaatctactt
    70381 tgctgaacag catcgccggt acattttctg tcgatcaggg tcaaatttta ttgaatggaa
    70441 aaaatatcac gaaaaaatct gttgtcgaac gttcgaaatc catcagtcgc gtattccaag
    70501 atcctaaact agggacagcc gttcgtttaa cggtagaaga aaatcttgct cttgctatga
    70561 aacgaggaaa aaaacgcggc ttcttccgtg gtgtcaaacc acaagaccga acatttttca
    70621 aagaccagct ggttcggcta aatttggggt tggaaaatcg tttatcctca gaaatcggtt
    70681 tgctgtctgg aggacaacgt caagctatta cgttattgat ggccacatta cagcgtccag
    70741 aactgatctt attagatgaa cacactgcag cgcttgaccc tgctacttcc aaaacggtga
    70801 tggaattgac agaaaagctg atcactgagc agaatctcac tgcttttatg gtcactcatg
    70861 acatggaaga cgcgatccgc tatgggagtc gactgatcat gctgcatcaa ggaaggatcg
    70921 tcgtagatat ccagcaagag gaaaaacagc agctcacagt gccccaatta ttagcgttgt
    70981 tcaaacaaaa cagcggcacg gcattaaaag acgatcaagt acttttatct tcataatcag
    71041 aaagaggatg ggacaaaaaa tgaacgccca ccctcttctt ttttctcttt ccttctttcg
    71101 tttttgcttt atgtatcaac cgttgtttct tcgaaaacta ttttgttctc tccttcttcc
    71161 tgatagaatg gatgggaaag gagcttgatg ccatgacaac gaaagacaag gtacttcaat
    71221 tattgaaaaa gacaccagat tttctttccg gagaaaagtt ggcacagcaa ctggaagttt
    71281 cccggacaag tatttggaaa gccatcaaag aattggaaaa ggcaggctat cgttttgagc
    71341 acgggcctac agggtatcgt tacctgccct ctgacgtact agatgcaaaa gaaatccagc
    71401 aagagctacg gtcgtttatt cctgatttga aagtacaaac gatgatgact tctgaatcta
    71461 cgatgaaaga tgccaaactg gcagcagctc aaggagattc tgtttcgaag ttatttgttg
    71521 ctgatacaca agaacaagct cgtggacgtt ttggacggcc ttttttcgct gaacctggac
    71581 gaggaatcta catgagtctc ttgctccatc cagacaaacg gtttgatgaa ttgcctcaat
    71641 atacgatcat cgcagccagt gcctgtgtga aagccattga atctgtaacc ggtcgttctg
    71701 cttcgatcaa atgggtcaat gatatctaca tggatggaaa aaaagtctgt gggattttat
    71761 ctgaagcagt cagtgatatg gaaagcggca ggatcagtca tatcatcatt gggatcgggc
    71821 ttaatttttc tgttcctcaa aatcattttc cagctgatct ccaagaaaaa gccatctctg
    71881 tctttcccga cggacaagct agtgtcacaa ggaatcagct aatcagcaaa atctggcaag
    71941 aattgttcac ttatctttct cacccagaaa aagcgttgga cttctataaa gaaaaatcat
    72001 tcgtattggg aaaaaccgtg tcgttcaatc aaaaaggaac gacttatgtc ggtcttgcaa
    72061 aagaaatcgg tgatcatggc gaactgatcg tccaaacaaa tgaacagaag atgagtttgt
    72121 cttctggcga aatcagtctc acatctatcc gtacgcaata attatcagat ttcacaaaag
    72181 gacacctgat ctcatacagt gtcctttttt ctgaattctc ttcaaaagac tctccttatt
    72241 ctatcagctg acagtttctc tatcctctag aaaaaggtat tctaaaaata tctataaagc
    72301 atggtacact ttctttaaat gaatcaatgc aaaaaacaag agagtcatca gtttacacga
    72361 tcaaaagaga tagcaggccg cttccttttt caatcttata gatagaccat gggtaacgat
    72421 aaaaacaaaa aagaacttgc gacttatttt gccgtaagtt cttttttagc tagtgcctta
    72481 atctcttaat cccaacaaac gtctcgcata gatcacctga ccagagtgca tagcagcatc
    72541 atcaataata gaaacgagac gcattcctcg tgtgactggt ggtgtccagt tctcgtcaat
    72601 tacttcatct agctcggaag gttccagcct tcttaagtaa gaaatagcaa aatcagcagc
    72661 tcctcgcaag tatcctagca gatcttctat ttgattagct tggatctgct ttgcttgttc
    72721 cagactatgc ttccaatctt gttcgttatc tgctatcgtc atcggaaacc ggctttccca
    72781 gtcttgacta tgccaaattg gagaagttcc tgctaaatca gcaatctgga aatctaattc
    72841 tcgagcggta tgccaagcca gccaagtcat tgatttgatc tgcggggctc tatcttccac
    72901 tggaaaacga ttggcctcct caatagagac gccttcaaac aaacgttcaa accgatcgta
    72961 tgcccgatcc acactctcgc atagcatatc taaataatcc atttattttc ctccttttta
    73021 tttatcctac ttactttttc ctaattctag gatacctact tatatgttac tacacaccct
    73081 tattttttac actttaacag tcaccatcat caatagtttg gagattgaaa gaaaaagata
    73141 gtgtcgaact atcaattgaa caagaagcaa caatatgaac gtttcattaa tattctttaa
    73201 ataaacaatg atttttcaaa cgaacagttt aagactagga gaaaaagagt ataataggaa
    73261 aatgtttgaa aggaaaggaa ggggaaaaat gaaaaaaagg gcaagtttac tagtcctagt
    73321 tgctttgttg ttcaatatct tttcgtatgg tgtggccgtt tcagcggaaa catacattcc
    73381 taaagaccct gggaatattt ataatgatct aactggtaac tatggcttgt taggaattgc
    73441 tagtcaattc cacgtatttg ctaaaaataa gacaactgtg aatgcacata cagacggaaa
    73501 tatagcaaca catgaacttg atgcaaaaaa taattttggt acagagcttc atgaaggttt
    73561 attgaaccaa gaaataaatt atatacaaaa aactgattct ttcgctaatt cttcaggcat
    73621 acctacaggt agcacaagaa tgaataaatt tgtcgttggc agtaacgtgg atgtagcact
    73681 tcaagatggt catcctacag tcaatggatc caggcttgat cacttaactc cagatgattt
    73741 ttataaagat cgtgctgggc atgaatatat tgatttcgag caggaatttg ctaaattgaa
    73801 tgtagcagca aatgatcttg caacgatcac tccagctaaa acatatactg ctgcagattt
    73861 tccagacatg aacaatcgga caatcgattt aactgattta tcagataccg ggtttttatt
    73921 ggtaaatatc gatgcagaag tgttaacgat gaatacgcca cttcaaatca ttaatccaaa
    73981 tgatcaagta gttgtattta atgtcatcaa cagttcttct gcattgaacg tacagtcacc
    74041 aatcaaatac aatgatcgtt caaatcatga aacagaagat ttttcggatg cgaatatctc
    74101 ttggaatttt ggaaatgaga tgactgattt gacaatcagt gctccttttc aaggaacaat
    74161 ccttgcgcct aatgctacta tccgcattac tcaaaaccaa gacggaacga ttataggaac
    74221 aaatgtgatt ttagatgcag ctaccaatcg ttgggatcct aacgaaatat ttatcagcga
    74281 caatggtaca gatacgacag acacaagcga cagtacagat acatctacca caagtgatag
    74341 ctcagattca tca
//
LOCUS       DACGLW010000005        61577 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.005, whole genome shotgun sequence.
ACCESSION   DACGLW010000005 DACGLW010000000
VERSION     DACGLW010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 61577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61577
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.005"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            complement(59..187)
                     /locus_tag="IXT53_000413"
     CDS             complement(59..187)
                     /locus_tag="IXT53_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HAQ4904272.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="IXT53_000414"
     CDS             complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="IXT53_000414"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAQ4904273.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(1674..2729)
                     /locus_tag="IXT53_000415"
     CDS             complement(1674..2729)
                     /locus_tag="IXT53_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAQ4904274.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="IXT53_000416"
     CDS             complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="IXT53_000416"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HAQ4904275.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="IXT53_000417"
     CDS             complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="IXT53_000417"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HAQ4904276.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="IXT53_000418"
     CDS             complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="IXT53_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HAQ4904277.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="IXT53_000419"
     CDS             complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="IXT53_000419"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HAQ4904278.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="IXT53_000420"
     CDS             complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="IXT53_000420"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HAQ4904279.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            10491..12050
                     /locus_tag="IXT53_000421"
     CDS             10491..12050
                     /locus_tag="IXT53_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HAQ4904280.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(12161..14092)
                     /locus_tag="IXT53_000422"
     CDS             complement(12161..14092)
                     /locus_tag="IXT53_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ4904281.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="IXT53_000423"
     CDS             complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="IXT53_000423"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAQ4904282.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(15021..15773)
                     /locus_tag="IXT53_000424"
     CDS             complement(15021..15773)
                     /locus_tag="IXT53_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ4904283.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(15932..16141)
                     /locus_tag="IXT53_000425"
     CDS             complement(15932..16141)
                     /locus_tag="IXT53_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4904284.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(16152..17480)
                     /locus_tag="IXT53_000426"
     CDS             complement(16152..17480)
                     /locus_tag="IXT53_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4904285.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            complement(17845..18432)
                     /locus_tag="IXT53_000427"
     CDS             complement(17845..18432)
                     /locus_tag="IXT53_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HAQ4904286.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(18515..19765)
                     /gene="clpX"
                     /locus_tag="IXT53_000428"
     CDS             complement(18515..19765)
                     /gene="clpX"
                     /locus_tag="IXT53_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HAQ4904287.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(20012..21301)
                     /locus_tag="IXT53_000429"
     CDS             complement(20012..21301)
                     /locus_tag="IXT53_000429"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HAQ4904288.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(21455..22387)
                     /locus_tag="IXT53_000430"
     CDS             complement(21455..22387)
                     /locus_tag="IXT53_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4904289.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(22544..23203)
                     /locus_tag="IXT53_000431"
     CDS             complement(22544..23203)
                     /locus_tag="IXT53_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HAQ4904290.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(23206..24231)
                     /gene="sppA"
                     /locus_tag="IXT53_000432"
     CDS             complement(23206..24231)
                     /gene="sppA"
                     /locus_tag="IXT53_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HAQ4904291.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(24244..24534)
                     /locus_tag="IXT53_000433"
     CDS             complement(24244..24534)
                     /locus_tag="IXT53_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4904292.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            24944..26749
                     /gene="glmS"
                     /locus_tag="IXT53_000434"
     CDS             24944..26749
                     /gene="glmS"
                     /locus_tag="IXT53_000434"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HAQ4904293.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(26821..28176)
                     /locus_tag="IXT53_000435"
     CDS             complement(26821..28176)
                     /locus_tag="IXT53_000435"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HAQ4904294.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(28200..29369)
                     /locus_tag="IXT53_000436"
     CDS             complement(28200..29369)
                     /locus_tag="IXT53_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4904295.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(29366..30253)
                     /locus_tag="IXT53_000437"
     CDS             complement(29366..30253)
                     /locus_tag="IXT53_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HAQ4904296.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(30442..34122)
                     /gene="nifJ"
                     /locus_tag="IXT53_000438"
     CDS             complement(30442..34122)
                     /gene="nifJ"
                     /locus_tag="IXT53_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HAQ4904297.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARAERRKKMEQATKEKKEK"
     gene            34320..34841
                     /locus_tag="IXT53_000439"
     CDS             34320..34841
                     /locus_tag="IXT53_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAQ4904298.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLSCPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            complement(35540..36105)
                     /locus_tag="IXT53_000440"
                     /pseudo
     CDS             complement(35540..36105)
                     /locus_tag="IXT53_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(36284..36940)
                     /locus_tag="IXT53_000441"
     CDS             complement(36284..36940)
                     /locus_tag="IXT53_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4904299.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRDILHDKELLEQFGPLDQNTINEEELLNQYNIRLVNWEISEPIVNNFGD
                     "
     gene            complement(37508..38443)
                     /gene="whiA"
                     /locus_tag="IXT53_000442"
     CDS             complement(37508..38443)
                     /gene="whiA"
                     /locus_tag="IXT53_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HAQ4904300.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(38477..39475)
                     /locus_tag="IXT53_000443"
     CDS             complement(38477..39475)
                     /locus_tag="IXT53_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HAQ4904301.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(39472..40356)
                     /gene="rapZ"
                     /locus_tag="IXT53_000444"
     CDS             complement(39472..40356)
                     /gene="rapZ"
                     /locus_tag="IXT53_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HAQ4904302.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(40491..41222)
                     /locus_tag="IXT53_000445"
     CDS             complement(40491..41222)
                     /locus_tag="IXT53_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HAQ4904303.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            complement(41226..42491)
                     /locus_tag="IXT53_000446"
     CDS             complement(41226..42491)
                     /locus_tag="IXT53_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HAQ4904304.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(42754..45570)
                     /gene="uvrA"
                     /locus_tag="IXT53_000447"
     CDS             complement(42754..45570)
                     /gene="uvrA"
                     /locus_tag="IXT53_000447"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HAQ4904305.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(45580..47574)
                     /gene="uvrB"
                     /locus_tag="IXT53_000448"
     CDS             complement(45580..47574)
                     /gene="uvrB"
                     /locus_tag="IXT53_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HAQ4904306.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQVRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKNAAKALDFETAANVRDMILELKASK"
     gene            47833..49335
                     /locus_tag="IXT53_000449"
     CDS             47833..49335
                     /locus_tag="IXT53_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ4904307.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            49337..50074
                     /locus_tag="IXT53_000450"
     CDS             49337..50074
                     /locus_tag="IXT53_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ4904308.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(50442..51608)
                     /gene="dnaJ"
                     /locus_tag="IXT53_000451"
     CDS             complement(50442..51608)
                     /gene="dnaJ"
                     /locus_tag="IXT53_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HAQ4904309.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(51759..53588)
                     /gene="dnaK"
                     /locus_tag="IXT53_000452"
     CDS             complement(51759..53588)
                     /gene="dnaK"
                     /locus_tag="IXT53_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HAQ4904310.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            complement(53639..54202)
                     /gene="grpE"
                     /locus_tag="IXT53_000453"
     CDS             complement(53639..54202)
                     /gene="grpE"
                     /locus_tag="IXT53_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HAQ4904311.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            complement(54296..55339)
                     /gene="hrcA"
                     /locus_tag="IXT53_000454"
     CDS             complement(54296..55339)
                     /gene="hrcA"
                     /locus_tag="IXT53_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HAQ4904312.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEEKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            complement(55574..56740)
                     /locus_tag="IXT53_000455"
     CDS             complement(55574..56740)
                     /locus_tag="IXT53_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HAQ4904313.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMRLMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYRDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            complement(56756..56983)
                     /locus_tag="IXT53_000456"
     CDS             complement(56756..56983)
                     /locus_tag="IXT53_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HAQ4904314.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            complement(57370..57626)
                     /locus_tag="IXT53_000457"
                     /pseudo
     CDS             complement(57370..57626)
                     /locus_tag="IXT53_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320124.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            complement(57969..58992)
                     /locus_tag="IXT53_000458"
                     /pseudo
     CDS             complement(57969..58992)
                     /locus_tag="IXT53_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325763.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            complement(59630..60304)
                     /locus_tag="IXT53_000459"
                     /pseudo
     CDS             complement(59630..60304)
                     /locus_tag="IXT53_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313658.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            60827..61417
                     /locus_tag="IXT53_000460"
     CDS             60827..61417
                     /locus_tag="IXT53_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HAQ4904315.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQKEGWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKKIRLYKLGFYDYYKKYGDPFS"
BASE COUNT        17091 a        13214 c        10655 g        20617 t
ORIGIN      
        1 taaaaagact aagaaacaat aacaaaagtt aacacttctt agtctttctt tagcacgtct
       61 atttattaaa gattttaggg ccttttctca tgtttttacg atcattctta ggtgtttcca
      121 tttgtttgga tcggcctcca ccttgtttct ttttgatcaa ttctttcatt ttttcttttt
      181 ggtccatttt tattctcctt taacgaatga agcgactaat tcgatatgat gggtttgcgg
      241 gaaaagatct acaggctgaa ctttcttcac ggcataacct gcttctgcaa ataacttagc
      301 atctcttgca aaagttgccg gattacatga gatatagacg attcgttctg gtgacatttc
      361 gattgctgat tcaataaaat gatgatctaa tccttttcgt ggagggtcaa ctacgatcac
      421 agatggtttg atcccttttt cttgccattt tttcataact ttttctgctt ttccgacttc
      481 ataatgaaca ttttcgattt tattatttaa agcattgaac tgtgcatctt caattgcttc
      541 tggaataact tccatcccat acaccttatc cactctgtct gctaaagaca agccaatcgt
      601 acctattcct gaataagcgt cgatcacaat gtctttttta tctaactcgg caaactcgat
      661 ggctgtttgg tacagatact ctgcttgttg agtatttact tgataaaatg actttgctga
      721 gattcgatag gtttttccta atagctgatc ttcgatatag tctctgccat ataaaacttt
      781 ttctttgtct cctaaaatca catttgtttt ttcttcattg atattttgga tgaccgaaac
      841 cacttctggc aattcttcat gaatagcttc agcaatttcc ttggatttaa agaatttttc
      901 tttacgagta actaaaacga ccatcatttc atgggaataa tgaccacgac ggataatgat
      961 gtgacgaagc tgtccttcat ttttttcttc attatatcct cgtacttgga accgttgcaa
     1021 gatatctctg actactttga ttgcttgatc gatgacagga tcttggatat aaaaatcttc
     1081 gatagggact agatcatggc tattcttttt gtaaaaaccg gtggctaact gtccatcgat
     1141 acggcgaaca gggatctgtg ctttattacg gtagccgaat ggttctgcca ttccgaccgt
     1201 tggaagtaca ggaacttctg gcatcttagc aatcttcgcc attacatgct ctacttgttt
     1261 ttgtttgaag acaagctgtt gttcatactt caaatgggct aaaggtgcga tacctatgcg
     1321 aagtaatgca ttgttttcta caggctgacg atcaggtgat tgtgtaataa atgtttcgac
     1381 ttttccatat ccaaacttat tgcctgcttt tactactagt atttctactt cttctccagg
     1441 gagtgcattt tcgataaata atggataacc atcgattttt gctaccccca ttccttcata
     1501 actcaagtcg acgatttcga ctgtatatcg ttcgtttttc tttactattt gctgttctgt
     1561 catattttgc tcctttaagc ctatttttca atcctactga actctactat aaatcagcta
     1621 attacacaac taaaaccggc actgacctta ctacgttcag caccggcttt tgattaattc
     1681 ttttgattag gtgtttcgtc tgctattttt ccgtcaccgt cgatatcttc atccgttaaa
     1741 cgttcgactt cttttacgaa ttctttgctg acaacctcta attcttcttc atctgtacct
     1801 gtgattgctt catcaggaat ctgatcggta ttggcaaaga actcaatgtg ttgatgaaga
     1861 ttttggaaat gcatcggtgc ttccccgccg tattcaccat ctaagttgat cattactggt
     1921 cgattgatat catcttctgt ttcaacgaac aaactgcttg ttttggtata gattattctt
     1981 ggatcatcga catgtcgacc gccattcagc atgagtgctg caatatgcaa gatctcaaag
     2041 atatttgctg tcttcacaat gattaaagaa aacttcccat catccaattt ggcatctggc
     2101 gcgatctgtt cgaatcctcc aacagagttc gtcagcccta ggaaaaacat cgaagcattt
     2161 ccgatatatt ccccttcatc atacttcaag cgcattttga ttggctttac tcgagggagc
     2221 atctctgctc ctttcgcaag ataagcaaga taaccaaaaa tactctttag ttctgatgga
     2281 acttcataag tcaattctgt caaatagcca cctgcagcga tattgatgaa gtaactgtta
     2341 tcagaaaggc cgatgtccat ttttacggtt tggtgttttt ttatcacttc tgctgctgct
     2401 ttaatattat ctctaggaat ttttaatgct cgagcatagt cgttcgttgt accagctggg
     2461 atgatggcca ttttaggacg gtgttcaagc ggagcgattc cattaaccac ttcgttgatt
     2521 gtgccatcgc caccagctgc cactactaga tcaaaaccta gttctgcgac tctacatgct
     2581 tcattcttgg ctgaatctgg ttctggtgta gttgcatagg cgctggcttc aaatcctgct
     2641 tcttctaatg cctgtaggat atccgccaaa ttttttttca ataattcttt tcccgaggtg
     2701 gggttataaa ttacgcgtgc ttttttcatt ttcagcctct atcgtttcgc taattcatct
     2761 ttaagtagct tattcacgac accaggattt gcttgtcctt tagttgcttt catgatttga
     2821 ccgactagga agccaaccgc acgatctttt ccatttttga aatcatcgac agattgttgg
     2881 ttgttatcca aaatttcatt aatgattggc agtagtttgt tcggatcaga taattgcacc
     2941 caaccatttt tttcaactac ttcttgggct tctccgccat ttgtgatcaa ttcacggaag
     3001 acttttttgg cgatttttga gctgatcgtt ccatcggcga tcaaacgaat cattcctgct
     3061 aagttctcag gtgtcaattt tgtctcagct aattccagtt tctcactgtt caagtaagca
     3121 gaaacttcac ccatcaacca gttagatgct tgcttggcat ctgcaccatt ttccaaaaca
     3181 gcttcaaaaa aatcagacat ttcttttgta agtgtcaata ccattgcgtc atactctgga
     3241 agtccaaact cacggatata gcgttcgcga cgagaagctg gcatctcagg caaactagct
     3301 tttacttttt gaatccattc gtcatcgatc acgaatttcg ggatatcagg ctctgggaag
     3361 taacgataat cactagaccc ttctttgaca cgcatcaaaa ctgttttatt cgttgtttcg
     3421 tcaaaacggc gtgtttcttg ttggatttct cctcctgaaa gaagaacttt tgcttgacgt
     3481 ttttcttcaa aagctaaacc ttttttcacg aaagataaag agttcaagtt tttcaattct
     3541 gctttcgtac cgaactcttc ttgtccataa gggcgaagag aaatattggc atcacagcgc
     3601 atagagcctt cttccatttt cacatcgctg acttctgtaa attggataat tgatcgcaat
     3661 gcttctagat aagcgtatgc ttcttctggt gaacgcatat cagcttcaga aacgatttcg
     3721 atcaaaggcg tgccttgtcg gttcaaatca acgtaagaat aaccgccaat accatgcatg
     3781 ttttttccag catcttcttc taaatggaca cgttcgatcc ggattttttt ctttttacct
     3841 tctacttcga tttcgatcca tccgtcatga ccgattggtt gatcaaattg ggaaatttga
     3901 tatgcttttg gattatctgg gtaaaagtaa tttttccggt caaaatgagt atctttagag
     3961 atttcacagt ttaatgccaa agctgctttc atcccgaatt ccagcgcagc tttattcatt
     4021 actggcagca cgcctggata accccagtcg atcacgtttg tattgctgtt tggttctgca
     4081 ccaaaatgag ctggcgctgg agagaaaatt tttgagttgg tttttaactc gacgtggact
     4141 tctagtccaa tgactgtttc aaaattcatt cgtctttccc tcctaaaata gctggttgtt
     4201 gtttatggaa ttctgttgct tgttcaaacg catatgccgc ttgatacatc gttttttcat
     4261 caaaataatt accgatgatc tgcaatccaa ctggtaatcc ttctgataaa cctgccggta
     4321 cagacatacc tggaagtcca gccaagttga cagggatcgt taagatgtca ctcatgtaca
     4381 ttgtgattgg atcgttaatg ttctcaccga ttccgaatgc aactgttggt gaagatggcc
     4441 cgatgatcaa gtcataatct gcaaagactt tttcaaaatc tcgtttgatc aatgtacgga
     4501 cttgtcccgc ttttttgaag taagcgtcat aataaccagc acttaaggag aatgtaccta
     4561 acatgatacg gcgttttact tcttcaccga atccttctga acgtgagttg acatatacat
     4621 catctaatga tttcacattt tctgagcgat aaccgtagcg gataccatcg aatcgttgca
     4681 agttggaact tgcttctgat gaggcaatga tataataaac agccacaccg tattttgaat
     4741 gaggcaagct gacttcttcc acaacagcac ctaatgaacg gaatgtttct gctgctttca
     4801 atacagcttc ttttacgcca ggggccacac cttcgcctaa gtattcttta ggtaaagcaa
     4861 tcttcattcc tttgatgtcg cctgtcagtc cctctgcaaa atcaggaact gaaacgcctg
     4921 aagaagtacc gtctttttcg tcataaccgc tgatagctgt caatgctaat gcattatctt
     4981 tcaccgtacg ggtcattggt ccgatttggt caagagatga agcaaaggca atcaaaccaa
     5041 aacgtgagac acggccataa gtaggtttca tccctacgat gccgttaaaa gctgctggtt
     5101 ggcggatact tcccccagta tcactaccta aagaaactgg aatttgtcca gctgcaactg
     5161 ctgctgcaga accaccagaa gagccaccag gtacttttgt tgggtcccaa gcattttttg
     5221 ttttcttgaa ataagaagtt tctgtacttc cacccatagc aaattcatcc atgtttagct
     5281 taccaacagg gatcatatct gcttgataga ttttatccat gaccgttgca tcataaattg
     5341 gttcaaaatt gtaaaggatc ttagatgcag cggttgtcaa gatatctttt gttacgatat
     5401 tatccttgat accaattgga ataccagcaa ggacgttgct ttcatcaatc cctttttcat
     5461 ctaaagcttt tgcctgttcc aatgcttttt cttcgttcaa tgtaatgaat gcttcgacct
     5521 caggctctgt ttctttgatt cgttcaaatg ttgcttttgt caaatcttgt acagtgattt
     5581 ctttcgatac aagcatatga tgcagttctt ctagagaacg attgtgtaat tctgtcatta
     5641 tgcgccagcc tccccattat cgatgattgc gggtactttg ataaagccgt cttctgtttc
     5701 cggcacattt ttcaataatt catcgcggtt ccatccagct tgcgcccgat cttgtcgcat
     5761 cacgtttact gtttcaacca cattcgacgt aaatggaaca ccttctgtat ctacttcttc
     5821 cagcatttca accatatcga tgattttacc taattggtcg gtaaaacttg caagttcttc
     5881 atctgcgaac gcaagttttg ctaatttggc aacgtgtttc acttgttctt cactaatcgc
     5941 catactaccc cttctttcta aacgagttaa aaactttgct ctctatttta acacaagagt
     6001 gccgtgtctg aaattgttta ttgaaatttg tctgaaaaaa ctcatttttc attcttctac
     6061 tgagcgactt ttagccaatg catctgccat ttgatcttct gtccaaactt cgattcctaa
     6121 ttcttgtgct ttggtcagct tgctgccagc atcttctcct gctaccacga tatccgtttt
     6181 tttcgatacg ctaccagtca ctttcccgcc aagattttcg atcgtctctt ttgcttcttc
     6241 acgagtaaat cgtgttagtt taccagtaag gactactgtt ttttctttaa aaggagactc
     6301 gacttcttgc aattgggcat ttcgaagccc tttgtattcg aaattgacgc ctgcttgttt
     6361 taattcattc atcaattcat ggacttcttc gttactgaaa taagtgacta cactgtcggc
     6421 aatgatatca ccgatcgtat caagcgcaat aatcgactca tagtcacttt tactcaatgt
     6481 ttctagatca ccgaaatgtt ctgctaaaat ttttgctgct ttcgctccga catgtcggat
     6541 tcctaacccg aaaatcaacc gctcgaccga attatctttg ctgttgtcga ttgctgtcaa
     6601 aatattattt gctgattttt cttttatttt gtccaacgtc aataactctt cttctgtcaa
     6661 tttgtaaaga tcagcaacat ctgctactag ttttttatca tacatctgtt ctagtacgcg
     6721 cggccccaac ccatcgatat tcatcgcatt tcttgaaaca aagtggttca aaccttcttt
     6781 catttgagca gggcatttag gattgatgca ccgtaaggct acttcttcat ccaaatggac
     6841 aagttcactt ccacaaactg gacaatgtgt aggcaattgg tattcttcgc tgtctttagg
     6901 tcgtttatct aacacgactt gcgaaacttc tgggatgatg tctcccgctt tataaatcag
     6961 aactgtatct ttcaagcgga tatccttcaa cttaatataa tcaccgttat gcaggctggc
     7021 acggcttact gtcgttcctg ctactcggac tggtgtcatg atagctgttg gcgttactac
     7081 ccctgttcga ccgactgtcc attcgatgtt ttcgattagc gtttcaactt cttctggcgg
     7141 aaacttgtaa gctgctgccc atctcggtgc tttgacagta aaaccaagtt gatcttgtag
     7201 cgagaattca ttcactttga tgacgattcc atcgatttca tagggcaaat ccacacgttt
     7261 gtcatgatat tcttcaatgt atgaccacac ttcatcaatc gaatgacata gacgtttttc
     7321 ccgattcgtg tggaatccaa ttttttctag ctcttctagc gcatcatact gggtttttgc
     7381 ttgcatagga ccgaaatcag ctactgtgta taaaaaagtg tccagatttc ttttcgctgt
     7441 gattttagaa tctaactgac gcaaagaacc agctgcggcg ttccgaggat tcgcaaagat
     7501 atcttttccc tctgcttctc tgtcttggtt caattggaca aatgaccgtt ttggcatgaa
     7561 acattcgcct cttacttcga tgttcattgg ttcttttaat ttgattggga ccgaccggac
     7621 agtttttagg ttttcagtga tgttttctcc tacagttcca tccccgcgag tcgcacctct
     7681 cacgaaattt ccatcttcat aacgcaaaga aacggaaagt ccatctattt ttagttcaca
     7741 gcaatagtca actacacgtc ccactgcttt ttgtacacgt tgatcaaaag ctatcagctc
     7801 ttctttgctg aaaacgtcgt tcaaactgta aagcggaata tcatgagtga ctttttcaaa
     7861 accttctaat acttttccgc ctactcgttg agtgggagaa tcagacgtga tcaaatcagg
     7921 atattgttcc tcaattgcta ctaattctgc gtattcttta tcataaacat agtcttccac
     7981 tgttggttta tcttctacgt agtattcgcg agaccattgg ttcagtcttg tacgaagttc
     8041 ttctgctcgt ttagcagctt cgtcaatcgt catttcatgt tccatcattt accctccact
     8101 actaagcttt ttcaattggc gcaaaggcag ccaaaagacg ttttactcct tgacttggga
     8161 aagccacatc tagttctaga tccttcgacg tcccgcttac tcggacgact gttcctagac
     8221 cccatttttt gtgtttgact ttatctccag gtttccagct gttattttcc cctccagtag
     8281 cacttttaga gggaacaacc gctgtctctg gctgtttata gctatatgct gtttttttag
     8341 cgaatacagg tgttttttta ggcgtaggac gcatgccttc gatttccagt aactcctgat
     8401 cgatttcttc tacaaaacga gaaggtcggt tatattgcgt ccgtccatat aatgttctgg
     8461 agaaagcatt tgttaaatac aacgcttctt ctgctcgcgt gatccccaca tatgcgagac
     8521 ggcgttcttc ttccagttcg ctttcttcca tcaatgctcg agataaagga aaaaccccct
     8581 cttctagacc aatcaggaat acaactggga attctaatcc tttggctgca tgcaaagtca
     8641 tcaatgtcac ttgggaagca tcttcttcta aattgtcaat atcggaaaca agtgccagat
     8701 catttaagaa gaccgttagt ttttcttctg gtgcatcagc gtcttcttca ttttgctgtt
     8761 cgaattgttt gtcgaattct tgtgtgaccg ttaaaaattc ctccaagttt tcgatgcgtg
     8821 cttgtgcttc taatgtattt tggattttta agtcttctag atatccgcta cgatccaaaa
     8881 cctctttcgt caattctgtc actgtcaagt atgggatcat ctgagtgact tcttggatca
     8941 tctctccaaa tgcacctaat tgctgtcctg cttttccacc gatatttgcc agatccacat
     9001 tttgcgctgc ttcaagcagt ggccattcat gaagagaagc aaaactgcgt agtttttcga
     9061 tagatcctgg tccgatgcct cgtttgggcg aattaacgat acgctcaaaa ctgattgaat
     9121 cttgaggatt tgccagcaca ttcaaatacg ctaaaatatc tttgatttct ttacgatcgt
     9181 agaacttgtg ccctccaacc attttataag gaatatttgc tttcaacaga gtttcttcca
     9241 ttacacgaga ttgtgcgttc gtccggtaca agatggcaaa gtcgccgtaa ttccgatgat
     9301 tgcttcgaat ctcttcttgc atccgatcta cgataaaacg ggtttcgtct cgttcattat
     9361 ctgcacggta gtaagtgatc ttgtttcctt ctttgttttc tgtccacaag ttttttggtt
     9421 ttcgattgct attgttttca atgacttggt ttgctgcgct aaggatgttt ttcgtggagc
     9481 gatagttttg ttctagcaaa attactgcag catctggata atctttttca aaatccaaga
     9541 tgttctgcat atctgcaccg cgccagccgt aaatactttg atctgcatct cccacgacgc
     9601 acaggttcct aaatctacct gccaataaat tgactagcgt atactgtgcg tgatttgtat
     9661 cttggtactc atctacatga atataatgga atttgttttg ataatatgtg agtgaatcag
     9721 gatgttcttc aaataatcga atcgtattca tgatcaggtc gtcaaagtcc atacattgat
     9781 tatattgaag ctctttttgg taagcagcat aacattttgc cgcaatctct tcgtaataac
     9841 tgccttgcat ttttgcaaaa tcctgagggg tctgcaggct gttttttgct tgagagattg
     9901 ttcctaatat acttcgagga tcatattttt tcggatcaat gttcagttct tttagtatcc
     9961 gtttcatcaa ggtcagctgt tcagaagaat cgatgattgt aaagttccgg ttatacccga
    10021 taaaatcaac atctcttctt aaaatacgca cacacatcga gtgaaaagtc gatacccaga
    10081 catcttcgcc tccagatgcg agaatcgcat tgacacgttc tttcatttct tttgcagcct
    10141 tattggtaaa agtaatcgct aggatgttcc aaggattgac ttctttttcc tcaatcaaat
    10201 aggcaatgcg gtgtgtcagc actctcgtct ttccacttcc tgcacctgcc attagcagta
    10261 atggtccgtc agtgtgaaga acagcttctt tctgacgggg gttcatcccg tttaataatt
    10321 cagctttgga tgacattaac aacatccttt cttttatcaa tcttgttaat tatagcattt
    10381 tcttggcttt cattaaacta gctagttcaa aatcatttgt ttctttttat ggatttttcc
    10441 ttgtcaattc gttgatttcg cgctaaatta aaagaaagcg aggcgtttga atggaaaaaa
    10501 aaggaaaaat caagaaatta gataagactc agatatattt tattctaaaa ggaatatttg
    10561 ttggactgct gacaggaatc gtcgttagcc tgtttcgatt atctatcgaa aagctaagcg
    10621 atttagttcg aagtatctat gaaatgagtc gggaacagcc gatttactta attggcctag
    10681 ctatttgctg catccttgca gcatttttcg tgggttatct ggtcaaaaat gaaccggata
    10741 tcaagggaag cgggattcca caagtagagg gacagcttcg cggggaatta tcaatgaact
    10801 ggttctctgt tttatggaaa aagttcattg gcggtgtact ttcagttggt gcgggccttt
    10861 ttctaggtag agaaggacct tctatacagc ttggcgcatc cattggacaa ggagctggac
    10921 aattgtttcg gagtccatct tcagaagaaa agattttaat ttctagcggt gcaagtagcg
    10981 gacttgcagc agcattcaat gctcctatcg caggattact ctttgtttta gaagaaatcc
    11041 atcactcctt ttctccactt gtttggctga catcatttgc ttcagcaatc actgctaact
    11101 tcgtctctct ttactttttc ggcttgagac ctgttttata tctaggtgac cttccttctt
    11161 tgccgttgaa atattatggt tccttagtga tcttaggagt acttctaggc atcttaggtt
    11221 ttatttatca gaaggtcttg ctggctttgc caaaatttta caaccgattg cctttaccag
    11281 cttattttca cgggttcgtt cctttcttgc tgattttgcc aattggttat ttatggccta
    11341 gtactctagg cggaggcaat caaatcgttt tagcttttgg acatgcttcc ttgccaatca
    11401 ttatgattat cggtttgttt gttcttcgat tcgttttttc catgatttct tacggtggtg
    11461 gtcttccagg ggggattttc cttccgatat tgacattggg ggctttaatc ggtagctttt
    11521 atggaaacat tatggttgat ttcgggatgg atccaatcta catcaaggat tttgtcgtta
    11581 ttgccatggc tggttatttc acagcgattg gaaaagcacc gctgacagca attattttag
    11641 tgacagaaat ggttggctct cttaatcatc tgatgccttt aggattggct gctcttgttg
    11701 cttatatcgt caatgacctt ctcggcggta atccaattta cgaatcatta ttggaacgtc
    11761 tacttggaaa tactcagacc gcctcactaa aaggtgcaag gacaactttt gattttccag
    11821 taacggcaga gagttcttta gacggattaa tggtacgaga cttcaattgg cctaaaaata
    11881 tgctgctaat ctctatacgc agaggaaatc aggaaatctt gactcacggt gatacgatca
    11941 tgaatgtagg agatattttg gttatcttga ctgatgaagc aaacttgcca agaatcaagc
    12001 aggaaatcga ggctaaatct tctatcacaa atttgaagca atcattttaa cgaacatgga
    12061 aaaactattt tacaacaact attcatctac actacttgat tctatatgat tctataaaaa
    12121 aagaagagac tgggaaaaac cagcctcttc ttttttattt ctatgcttct gctgctgcag
    12181 gagcttttct caataaacca taaatcacac cagatacgac tgctccgata gcaataaaca
    12241 ccaaatacat gaccgggtga ctcagtaaga atacaacgaa aatcccgcca tgaggagcca
    12301 gtaatttgat cccgaaagct ccgactaatc cgccagttaa agcagaacca ataataaaac
    12361 ttggaatggc acgaatcggg tcagctgctg caaatggaat cgatccttcc gtgacaaacg
    12421 atagtcccat cacgatgttc gtcaaaccag catcttgatc tgttttgctg aatttattct
    12481 taaacaaacg tgtagcaacg aaaattgcca atggaggtac cattcctcca gccataacag
    12541 aagccatcac gacacttccg ccagttgcaa cagtcgtagc taaagttgct gtaccaaaca
    12601 cataagcggc tttgttgatt ggaccgccta aatctgccgc catcattcca gctaataatg
    12661 caccaagtaa agcagcattc gaaccactca tacttgtcaa gaaaccgttc aatgcatcat
    12721 tgattgcttt catagggaca ttgaccagca acatcaagaa accagtaatc agtaagccaa
    12781 acactggata aaagaggatc gttttaattc catccaatga ttttggtaag ttcttgaata
    12841 atttacgtaa aaatacgatg acataaccag ctaagaatcc accaatcagt gcacctaaga
    12901 atcctgcacc gcctgtatta gctaatgcac cagcagcaaa tccaacgatc aaaccagggc
    12961 ggtcaccgat acttgaagca ataaatcctg ctaataccgg cagcatgaaa ccaaatgcag
    13021 cttgtccgat ttggttgaac cagctagcag cttgattgta attccctaga tttgataatt
    13081 gatcatgtgg cacgccaata aattggtcga tcatgaaaga aagcgcaata gcgattcctc
    13141 caccaataac gaaaggtaac atgtgtgata cgccgttcat caaatctttg taaattcttt
    13201 gtccgatagt accatccgca tttccttctt ctgaactgct ttcttttcca tcaccatgga
    13261 atactggtgc agtaccacta agggctagat tgatcagctc ttctggttta cggataccat
    13321 cactgacagg acgattgatc agttcttttc cgtcaaagcg attcatctct acttttttgt
    13381 ctgctgcaac gattacacct tctgcacggg cgatgtcttc agcagtcaat cggtttttga
    13441 ttccttctga accgttcgtt tcgactttga tctccacacc catttctttc gcttttttct
    13501 tcagagcatc ttctgccatg taagtgtgag cgatccctgt tggacaagct gtaacggcaa
    13561 caacgaattt tctttctgga tgagcagaag cttctgcagc ttgtgtttct tgttctgctt
    13621 tttcttctgc ttctttttgc tcttcagctg tttggaacaa gtcttggact tcttctggtg
    13681 tatttgcttc ttttaatttt cctacgaaat ctgcatcaat cagcaatcga gatagtgctg
    13741 ctaatgcttg caaatgtgtg tcgtttgcgc cttctggagc tgcgatcatg aaaaacagat
    13801 aagttggctg tccgtctaag gcttcataat ctactccttt ttggcttttt gcgaataaaa
    13861 ctgttgcttc tttaactgca ctgtttttcg cgtgaggcat ggcgattcca tcaccaagac
    13921 ctgtagaagt ttgtgcttcg cgggctagaa ttccttcttt atatgtttcg atatcggaaa
    13981 tccggccggc atcaaacatt ttttggacca tttcatcgat tgcgcctttt ttatcagtcg
    14041 cttgcaaatc catgatcatc gcttctttga ccattaaatc cttgatattc atgattatcc
    14101 tccttgaaac taatcaattt ttgtgatttc aacttctggc agcaactcat caatcaattc
    14161 gcgagctgcc aagtcatcag aaaaagctgt tgcgcttccg caagcaactc cccatttaaa
    14221 ggcttcgatt ggatctttcg tacggctgta agccccaata aagccggcaa tcattgaatc
    14281 ccctgcacct actgaatttt tcaatgggcg aactaataca ttcgttttat aagttccttg
    14341 ttccgtgaag agcaaagcac cgtctccagc cattgagatc agcacattct gtgccccttc
    14401 agctagcaat tttttaccat acggaatgat ttcttctaca gattggaagg ttgtattgta
    14461 aagctcagct aattcgtgat tgtttggttt gatcaacaat ggtttcttaa caagtgcatt
    14521 ttttaggtca tcacctgttg tatcaatcac gaaatctgca cctttttctc gaatgatatc
    14581 aatcagttct tgataaaatc cttttcgcag gctagccggc gcgcttcctg acaatacgac
    14641 gatatctcct tgtcctactg ttcctaatgc ttgcttcaac tcatcgattt cttcatctga
    14701 aatagatggg cccaaaccgt taatttcagt ttcagtatct gattttagtt tgatattgat
    14761 tcttgtatct tcttttacgg ttgtgaacaa agactggatt tgttcttttt caagccactt
    14821 cgaaataaat tcgccagtga accctcctaa aaaccctagt gctgtagatt gagaatcgat
    14881 ccgtttcaaa atacgggaaa cattgatccc ttttcctcca ggtaacttga agtcttcctt
    14941 cattcggttc aaatcgccta gttttaaccc atctacgcgt acgatataat caattgatgg
    15001 attaagtgtt actgtgtaaa tcattgtatt gcctccttaa ttgttgtctt ttcacggaat
    15061 tcattaacga attctaatgg acaacgctcg gttatgatcg tggctgcttc caaaggtgca
    15121 acagaggtga aggacaactg gttgaatttg gtatgatcaa tcaatacaaa tgtctcttcg
    15181 gaatttttca tggctaaacg tttaacagct gcctcttctg ggtccggagt cgtgaatcct
    15241 tgttcaagat gggcaccgtt cattcccata aatgctttat taaaatgaaa attttttagt
    15301 tgttctatcg cattagatcc taaaacagca ttagttgcca gctttatcgt tccacctaaa
    15361 ataatggtag caagctccaa atcaactaaa tttgccgcat gttgaacgga atttgtcact
    15421 actgtgatct gttttccttg cagaaaagga atcatttcta acgtagtaga gccagcgtct
    15481 aaataaatga catcttcgtc attaatggtt tcagctgccg atttggcgat ttgctgtttt
    15541 tcgtgaacgt ttttgagtga tttttcactc atattctgct caaaattcaa taattgctct
    15601 ttcttagctc cgccatgaac tctttctaga aacccactat tctccagctc ttgtaaatca
    15661 cgtcttatcg ttgattcaga agcattaaaa aggtttacaa gatcttgtga tttcacgatt
    15721 ttctgctgtt caagcaagtc taggattttt tgtctacgtt cctctgtaag cattttcacg
    15781 atcacctcaa tacaaataat agcatatttt ttttctactc gcaatataaa tcagtcaaaa
    15841 acgttcataa aaattataaa tcagtcaaaa aaaaataaaa aaccagtcaa aaagactggt
    15901 tttttatttt tttgtctatc atcaagccgt tttatcgttt tttattttcg cttcttgttt
    15961 ttttcatata aacagaagca ggttttcttt ccttatgaag aagataataa taaagtccca
    16021 tcccgccaaa catgaccaaa aaaccgatga ctgatcgaat aggaaaatta ggaacaaaaa
    16081 agagacagac ggataataat gatgccggaa ccattattcg ataatagagt tgtttatcca
    16141 ttttcttcac ctcatatttt ttgtaaagta atcaattgtt ctaattctcg gtaatctaaa
    16201 aattgcacac gcagatgctg ctcacctttc ttgacatcga attggatact tcgccgcttt
    16261 ttctttattc gtttgaaggt aacatcagag agtctatgtt tgatttcctt taacggtaat
    16321 tctgacaggg attctgaagg aacatagatt cttaaggata aaagatactc ttgatatttt
    16381 tcatctgtta cctctacttc tagatattcc ctaatataat aacaataagc ttcgattcca
    16441 ttatccaaag caacagtcgg atcgataact ttatagcaca tcatgcgcga gcgattctcg
    16501 tcaacccaag gaaaatacgc ctgagcattt agtgttccgc gaacacgcct ttctttataa
    16561 tctccttgtt gaatatcata actttttgtt acaaaaaatg aagcattctt tttaggaaca
    16621 ggttgcattt gtgcattctg atactggaca gattggatcg tatggtcagt gtaaatgtat
    16681 tcaacgaatg gcggtttcaa taaaccgaca ccattgtccc aagcaagatg atcaaaccca
    16741 agctggtccc taaaatagga ttgaaacatt tgattgtttg gcttttgcca gttaaagggg
    16801 tctcgataaa gctggataca tatgacttcg ttttctaatt ccttcacttt ttctacttgt
    16861 tggatgtcga gaaaaatcgg tttagggatg atttgatgat aatagcgact ataatacatt
    16921 tcccaacacg aagtgaaaca taaaccttgg taaaaaagat cgtatcctgg aaattggttg
    16981 caatcgacta ctacttcccc gttttgttgt ttcattttag gtaaatgttc gatttcatcc
    17041 tttttctcaa acaatagccg ttgagagatc tgttttgtat tgtgatagat aaactcgcta
    17101 taagctcgca tataggcgaa caccccatga gcaatcattt ttttctgaat gaagtctcga
    17161 atcaaaaatt gcttttcttt gaaaacagag atagacaata cattttttat caataattga
    17221 ttatcttcca agcgaaaact acattcgttc tcgtcatttc ctacaactaa ccaactttcg
    17281 tctcttaccg atgctagcat tattttttct tgaagctttc gaagatcata cggatccgct
    17341 tgttgtgtga aagagcggat aaatgttggc tggatcaaca gacgtgtcaa aatagcccaa
    17401 tcgcttattt cctgtttttt ttcttcttca tttaagtctg tgagctggat agtcatcaat
    17461 aatttatctt cgccaatcat tttctcacct ctttaaaatc tttttataat ttaattataa
    17521 ccaacgataa agaaaaacta aaactttatg tcttagaaac caatttggac gattacatga
    17581 tagactaagt aaaaagaaac aaagaggtgt ttacaatggg gttgaaattt gaacgtacag
    17641 ataatttaga taaacttttc gaatcatttg ctgtcgatcc ggaaaaaaca gttgcaccaa
    17701 aagaagagat cgatcgattt ctaaaaccac agccacaaaa tgaaaagaaa aaagacaaga
    17761 aacaaaagta agttaagctc aaaaaaagac ttaccagcca aacttttatg aaagagtttg
    17821 actggtaagt cttttttatt ctttttatga ttcaattgct tgttcgattg ctgcccacgc
    17881 ttcttctttt ccctgttttg taacggaaga aaagagaata aacgtgtcat tgcgatcaaa
    17941 atccaacgct tttttgattt cgctctcatg cttgttccat ttaccacgag ggatcttatc
    18001 tgccttagtg gcaacaacaa ttacagggat atcataatac ttcaaaaatt gatacatctg
    18061 gatatcttct tgagaaggct tatgacgcaa atctactaaa gaaacgaccg cacgtagctg
    18121 gtctctagtc gttaaatatg tctcgatcat ctttccccat tttgctcgct cagttttgga
    18181 tacttttgca tacccatatc ccggaacatc gacaaaatgt aatgcgttct cgatcaaata
    18241 aaaattcaat gtctgggtct ttcctggctt accggaagta cgggctaaat tctttcgatc
    18301 aatcaatgtg ttgataaaag aagatttccc tacatttgat cgtcctgcca gagctatttc
    18361 tggcaattgt gtatctggat attgttttgg tgccactgca cttatgataa tttcagcttg
    18421 atggactttc atggtctctc tccttaacaa ggtgatgaat aaagcaagcg gctgtcgcaa
    18481 aagccaaaat gacttttacg gcagcctctt ttcatcaacc cgcttttttc tctgaatctt
    18541 tatagattac tgttggctca ccagacaatt cagctgcttc ttttgtgata atcacttttt
    18601 cgatgttttc atttgaaggg atatcaaaca tcacatccat catgatttct tcaatgatgg
    18661 aacgaagtcc tcgagcaccg gtattgcgtt cgattgcttt tttcgcaatg gctctcaacg
    18721 cttcaggttc gaattcaagt tcggtattat ctagagaaag aagtttttga tattgtttga
    18781 ctagcgcatt tttcggctca gtcaagatcc gtaccagatc atctgttgtc aatttctcta
    18841 aagctgccat aacaggtaat cgtccgataa actcagggat caatccgaat tttagtaaat
    18901 cttcaggaat gatatgctgc atgacacttt cgtcttcaga aagtttttgg ttttgtgtac
    18961 caaatccaat cgtcttttcg cccatacgat tcttcacaat cgtttcgatg ccatcaaatg
    19021 caccacctac gatgaacaat atgttcgtcg tatcaatctg aataaattct tgatgtggat
    19081 gtttacgtcc tccctgaggt ggcacgcttg caacagttcc ttctagaatt ttcagcaagg
    19141 cttgttggac accttcgccg gaaacgtcac gagtaataga tacattttcg cttttacgcg
    19201 caattttatc gatctcatca atatagatga tccctttttc tgcacgttcc acattgtaat
    19261 cagcagattg aagcaattta agcaaaatgt tttctacatc ttcccccaca taaccagctt
    19321 ctgtcaaact tgtcgcatct gcgattgcaa aaggtacatt caatgttttt gctaatgttt
    19381 gtgctaaaaa tgttttacca gaccctgtag gtccgatcaa gcagatattg cttttttgca
    19441 gttcaacatc atctgtgttt tcttgtgctt gctgattgac acgtttatag tgattgtata
    19501 cggcaacagc taacgagcgt tttgcgcgat cttgtccaat cacgtagtta ttcaatactt
    19561 caagaatttc tagtggttta gggacttcag tgaattcgcg tacagcttct tcataaaatt
    19621 cttcatcaat gatttcttta cataggtcga tacattcatt acagatgtag acccctggac
    19681 cagctacgat ctttttcact tcttcttggg ttttcccgca aaaagaacag cgtacggtct
    19741 cattgctact tggattgtca tacatggtct tcacccctta acttgcaaaa agtatgcaag
    19801 aaactatcaa agactttaca gtcttttact atcataacat agtttagtaa aaagaaaaag
    19861 tgtttaacaa aggatcaact gacctatacc tttgcatttt ttactcttaa caaaaaataa
    19921 ggaactgtga caaaatcgtg tcacagtccc ttatttcgaa taaacaattt tctagaagta
    19981 gcttcttcag aaatgattta ttacttgatt tctattcttt cgcttctgtc gctgtacctg
    20041 tgatcaattc gattgctttt ttcattgtca catcatgttc cagcatatct tctgtcaaca
    20101 cgcgtttgat ttgttcgatt ggcatgttgt atgtttctga taattcttgg atttctgcat
    20161 tgatttcttc ttctgttgct tgaagatttt cagcagctgc aattgcttcg atcacaagat
    20221 ttgttcttgt gcgcatttct gcttcacctt cgaattgttt atgcaagtct tcttctgtag
    20281 aaccagttaa ttgatagtac atttcaggag agataccttg acgttgcatg ttgttcaaga
    20341 attcgtccat tgaacgatgt acttcatcat gaaccatcac gtgtggcaat tcaacgattt
    20401 ctgcgttttc aacagccata cggatcgctg cttcatcttt tgcatcgtcc gctgcttttt
    20461 cttttgtttc tactaattct ttgcgatatt tttctttcaa ttcgtctaat gttgatactt
    20521 cgtcatcaac atcttttgcg aattcgtcat ctaattcagg cacttctttt gcttttactt
    20581 catggattgt cactttgaag actgcttctt ttccagcaag atcttctgct tgataatctt
    20641 ctgggaaagt tacatttaca tctagacttt cacctgattt atgaccaact aattgatctt
    20701 caaaacctgg aatgaatgag cctgaaccta attcaagtga gtaattttcg ccttttccgc
    20761 catcaaacgc ttcgccatct acaaagcctt cgaagtcgat tactactgta tcgccatttt
    20821 cagctgcagc atcttctttg ataactaatt cagcttgtgc ttcttgttca cgtttgatac
    20881 gtgcatccac gtcagcatct gttacttcac ggtcttgttt ttcaacaact agatctttat
    20941 attgtcctaa ttttacttct ggtttcactg ttacttcagc agtgattacc cagtcttgac
    21001 ctttttccat gctttcaaca tcgatttttg gttgagaaac aggatcgatg ccagcttctt
    21061 tgacagcagc ttcataagct tctggtaata cagcattcaa tgcatcttca tataaagctt
    21121 cttcaccgta catacggttg aatacttgac gagaaacttt ccctttacgg aatccaggta
    21181 cgttcaagct ttttttcact ttattaaacg cagttgttaa tcctttttgg atttcgtctt
    21241 gcgcaatcgt aaattttaaa acaccatcat tcgtgccttt tttttcaaat gttgcagtca
    21301 tttaattccc tccgagttta gaaattcatt ttatacatca aaaacgtttc atgcatacct
    21361 ttaaatagta cactaaccct ttcaaattgt aaacatattg aatggataga atccttgttt
    21421 ttcataacat ttttatcttt ttcactgata aaatctaatg gaagaagatc atttgctgca
    21481 aaattttttc tctttttttt aaaaaatctt gtttttcatc ttctgtacct tcatacatcg
    21541 tttttcctgt acacgcctct acagtcagtt gaaaccagcg cttcgggtct tcgatgtatg
    21601 tatcatagac aggataaaca attgcagatt gcaacgtgaa ttcttcattt aacacggaac
    21661 taagcagcgc atcttcatta ccgtaacgat ctgaaaatac acataattgc tggtaacttg
    21721 cctgctgctg cggctttggc agatcttttg gtatgacttt gactaacttt tcttcaatcg
    21781 tcaaatagaa aacaggatgg cattcgttca attgcctaag tgtctccagt aattttgccc
    21841 ttgctaaaat atggacctct ggcgatacaa taagtttact cgctgtttgg ataaactcat
    21901 gataaggaag ctgttcgatc tcatcaatag acgcaagctg acgtgcaggt tccaacgaag
    21961 gaagttcctc cgatttattg tggagtgcct tgatcttctg cctctccatt tgtccggata
    22021 attcttctgc aaattggatt ttttcaatga cccgctgctc gaaagcttca taaaaatctg
    22081 tacttgctat caattttcta gcatataaaa aatcttgtgt actgattaac aagtcaaaat
    22141 aaaattcagc aaactcctca ttttgaagat actgtttttc atgcaagagt gcttgcttca
    22201 atgcttcttt tttctcgtcc aactcgtaaa gacaaaaaac aagcaggcta ttagcctcaa
    22261 agctttcttt caactcgtaa gctcgttgaa ataattcttt tgcctgctga tattgctggt
    22321 ttgctagtgc ttgttgtgct actcgcatta aattctcata tttttctgga aaatcaatcg
    22381 gcttcacgca gtgtcctcct cttgctcatg ttcagctcat gttcaatagt agcaaaacaa
    22441 aaaaagcatg acaattaaaa ataactgaag caccctctaa aagctagatt ttagatctga
    22501 cttttagaag gtgctttcaa ctgttactgc tttttattgc tcatcagctg ttgacttcct
    22561 ggttgaatgc tttgatcaga ttgatcgtaa caacacttgt attagtgaaa aaatcgccta
    22621 gatgttgctt tttctgatta aagattgctg gaagataccc taacatcagc aaaggaaatt
    22681 ttagaatgaa ccggcaagca ccttctctaa ctaaaacggt tgaccagcta agttcttctt
    22741 catcaaagct gatgactcga ataccaaaga tcattttccc aatcgtttga ccatgattga
    22801 atttcgtcaa taaaataaaa tacgctagat aaataagtaa ggctgtgaat ccgtagatac
    22861 tcagataaga atctgaaaaa ttaagccaac ctagattttt ggcaatacca agtgttaaac
    22921 gcgtaatagc tccgatacac aaaagatcga ccaagaaagc aaacattcgg ataaaaaaac
    22981 ctgcatagaa atacttagga aaatcatttg cttttggcgg ttcatttctt gtgtccgtat
    23041 actttttcca tttacgttgt ttctttttga tttcttcggg cgtcaaaggc tctctttcaa
    23101 aagagctcag taccttttgg gaaaagttgg agtcaggtcc tacagcaggc tgctgttgct
    23161 tatctttttc ttgttcctta ttttcatttg aaagctctga catgcttact cacctccgta
    23221 ataatacatt gcttttggtg attccgctgt ccctagattc tcaagaacgg ataagatctg
    23281 actagtctca gaaggcttca atccctggaa ttctgctagt ttacttccta accatgtact
    23341 agcaaaccca gatggcgtgg aagaatattc tactagttcc gcatcttcaa gctgttgttc
    23401 ctttcgcata gcttttaaag catcttcagg aaatcccaat tcatcgacta aacctacttc
    23461 ttttgcttgg acaccatcat aaatccttcc atcagcaatt tttttgactt cttcttcaga
    23521 tttattccgt ccctcgctga cgattgctac aaaacgacta tatgcgctgt cgatatatgc
    23581 ttgaagcacc gcttcttctt cttttgtctc tggtcgcgtt cctgaaccca tatctttcaa
    23641 cgctccgctt ttaactgttg tatcttccac acccagtttt tctagaagtc ctgaataatt
    23701 caatcctgac atgatcacac caattgagcc ggtcactgtt tcttctgttg caaagatttt
    23761 atctgcttga gcagaaatat agtagccacc gctagctgcc atgtttttca tgctgacata
    23821 cattgggata tcatgttctt tacgaatcgt atccaatttc ttagcgattt ccgcactttc
    23881 gtagacaccg ccaccaggag aatttacttc taaaaagata cctttgactg ttggatcttc
    23941 ttcaatcgcc ttcagttgtt ccataaaatt tttatgattg taaccttcag aagcaaataa
    24001 acctgaagac cctcccgcag caatcgttcc atctactgat aattttacga ttttttgcgt
    24061 actgtctccc tcttccaaca catttgaagc tagttcttca tccccatata accaactgtt
    24121 caatccctca atttgatcgt tttcctgagg cttagtcaaa ctagctgaaa ctaacgaaac
    24181 cacaaataat cctgcagcaa tcagaactgc tgcccaacgt ctcttattca ttctttctcc
    24241 tttctaatac aagtttttta tgacgatcac agtttctgac tgctcagaag tgtcgaaccg
    24301 ctctgctttt tcttcccgcg catacccgga aaacgactct tttgtcaatt catcttctac
    24361 cgtaaactca cgagcttcag caacttccaa ggcatcaggt gcgatttctt ggatgctatc
    24421 ccagccagta tctaaaaata ttttcgtatc atctgataca ccagctgatg cttcgaattt
    24481 ggacaattgt ttttttaaca ttcccagtgt catgatctca atttttgaat ccatttttac
    24541 tcctcctcta tccgattact ttctattgta ccgaactcct gaaaaagatg atagataaaa
    24601 acccattatt ttttgacata aaaagagcat ttgacaaagc atttgatata taccaattta
    24661 ttttttattc tttttttgtt gacaagaaca ttcgcaaaag gttatgattt taaatgtttc
    24721 cagataaaaa acaaaagagg aggatagtta taccttttag gatagcgcca ggtctgtgta
    24781 aacagatgac gaggagaggg tttatcgaaa gattcggcgg atggccctag ggactgcact
    24841 ctacagaaaa tccacaaaaa atatctgcaa agataaaaca aaaggatttt cctgcagctg
    24901 gaagcattca tacgaattca ttatagaaag taggatgttt tttatgtgcg gaattgttgg
    24961 aatgattgga ttaaaaaatg taacaccagg attgattgac ggtctagaaa aattagaata
    25021 ccgtggttat gattcagctg gtatctttgt ctcagatggc acaactgatt atctggtcaa
    25081 agcacaaggc agaattcaaa atttgaaaaa caaaataaat attgatacta caggaaatat
    25141 aggtattggt catacacgct gggcaactca cgggcaacct tctgaagaaa acgcccatcc
    25201 acatacttcc caaagtggac gcttcgtgct cgttcacaat ggcgtaatcg aaaactttga
    25261 agaacttaaa attgcttatt tagcaaatga ccactttatt ggtgagaccg atactgagat
    25321 tatcgctcat ctaattgaaa ctttcgcgaa ggatactaca acgcaagagg cgtttttaaa
    25381 agctttgcgg gtcatcaaag gttcttacgc tttcgctttg attgatcgga cagcacctga
    25441 tgttatttac gtcgctaaaa ataaaagtcc actattaatc ggactcggcg atggattcaa
    25501 tgtgattgcc agcgatgcaa tggctatgct tgctcatacg aaggaattcg ttgagattga
    25561 agatgaagag atggttaccg tcacatcaga aaaagttatt attcaaaatt ttgcaggtga
    25621 catcgtagaa cgttcttcat tcgaagcaca agtagattct tcagatatcg agaaaggtac
    25681 ctatcctttt tatatgctga aagaaatcga cgaacagcct attatcatgc gtcgtatcgc
    25741 tcaaaaatac gtaacagaag acaatcgtgt ctctcttgat caaaagctgg tcgatacatt
    25801 gtctgatagc gatcgaatct atatcgttgc ttgcggaact agttaccatg cggggttagc
    25861 aggtaaacag actctggaaa aattgacaca gatccctgtt gaggttcatt tagccagtga
    25921 atttggctat aacacacctc ttctttcaaa aaaaccattc tttattttct tgagtcaaag
    25981 tggcgaaaca gcagatagcc gccaagttct ggtaaaaatc aaccgtctcg gctacccttc
    26041 acttacgatt acaaatgttg ctggttctac tctttcgcgg gaagcatcct ttacactgct
    26101 attgcatgct ggacccgaaa tcgcagtagc atcaacgaaa gcttatactg cacaaatagc
    26161 tgtactaact ttattagcca aagctatcgg tgataaaaaa gaatttgaaa cttctcttgt
    26221 attcgatatc actcatgaat tgagtttagt tgctaatgcg atggaatcgg tcatcgccca
    26281 aaaagattat ttagaagagt tggcagaagt atatctcagc gatacaagaa atgcctttta
    26341 cattggacgt ggcgctgact acaacgtttc tcttgaagct gctttgaaat tgaaagaaat
    26401 cagttatatc caggcagaag gttttgccgc tggcgaattg aaacatggaa cgattgcttt
    26461 gatcgaagaa ggaacaccag tgattggaat catcagtgaa gaagtgactg gtgcgcatac
    26521 tcgaggaaac ctgaaagaag tcgaaagccg tggcgctaaa acactagtta ttgtctcaga
    26581 gggattagaa aaggaaagtg accagctggt gctaccaact gtccaccctt atctaactac
    26641 tctaaccaca gtcgtaccga ctcaattact tgcatattac gcaacgctat tacggggatt
    26701 cgacgttgac aaaccacgta atttagcgaa atcagtgaca gtagaataat aaaagaacaa
    26761 ggaccagtgt cagacatctg acactggtcc ttgttctttt attttttatg aaaatatctc
    26821 ttaatccagt ccgatttctt ctttgactac tgccgcgatt ttatcgacat agtaattaac
    26881 tttctcatca gtaggcgctt ctgccatcac tcgaagtaat ggttctgtac cggaaggacg
    26941 aactaggatt cttccttctg atcccatttc agcttctgcc tcttcaatca cttttttgat
    27001 tgccggaaca tccatcgcac cgtttttatc acttactcgg atattcacta atttttgcgg
    27061 atagattgtt acctctgctg caagctctga taatttctta cctgtctgtt tcatgatatt
    27121 cagtaactga attcctgaaa gcatcccatc tccagttgtg ttaaaatcta agaaaatcat
    27181 gtgcccagac tgctcgccgc caaagttata atcgtttttg cgcatttctt ctacaacgta
    27241 acgatcacca acttgtgtaa cgacatcttt caaaccgatt ccttccaccg ctttgtggaa
    27301 tcctaagtta ctcatgacag tcgtaacgat cgtatctttt ttcaatcgat ttttttctgc
    27361 caagtattta gcacagataa acatgatttt atcaccgtcg acaatgtttc caagttcatc
    27421 caccgcaata atacgatcgc catcgccatc gaaagctagt ccagcatctg ctcctttttc
    27481 aacgaccatc tcagctaaac gttctggatg ggtagagcct acgccatcat tgatatttaa
    27541 cccgtctgga tttgtgccca tggtataaaa atctgtttca agatcggcaa acaaacgatt
    27601 aacacttgtt gaagttgctc cgtttgctgc atcgacacaa atggtcaaac cggaaaggtc
    27661 accgttgatc gtttgtacaa ggaattgaga atatttcaat aagccttctg gaaattcttc
    27721 tacagttccc aatccttcag ctgatggacg aggcaattca tccacttcag catctaataa
    27781 tgcttcaatt tctagctctt ggtcatctac tagtttaaaa ccatctgcac cgaaaaattt
    27841 gattccatta tcttgtgctg gattatgaga agcagaaatc atgacccctg cgcttgcttt
    27901 ttgcaaacgt gtcagataag caacacctgg tgtagagatc acacctagct ggaacacttc
    27961 gatacccacc gataaaagtc ctgaaatcag cgcttcttct aacatttgac ctgagattct
    28021 tgtatcccgc cctactaaca cgcgtgggcg ttttccttct tcttcatgct ggcttaggac
    28081 ataacctccg caccgtccca gcttaaatgc aagttctggc gtaagttcct tgttggcttc
    28141 accgcgtacg ccgtctgttc caaaatattt acccatagtt gtaaaattac tcctttaact
    28201 taattttctg aggaatttgt tctgctcatt tgtgttgatt cgcttgtttc tgatgtttct
    28261 tctgtacttg tggtcgagct gcttgtccca gattccgaac tggaagaaat ggttgttgtc
    28321 gatggttccg ttcctgaatc agcagatgaa atacttctac tcgtacttgt attttcttgt
    28381 gtccgactgc tcgtcgaagc agagctactg cttgacgttt gcaacacggg tgtaatctcc
    28441 acactaactg attcgggctt gatacttaac ccctcttcta ctggtatttc aattttacga
    28501 gttgttcgat gattgatacc tgagacattg atcggtacag caatactgtc accgatcgat
    28561 gcaagcaaat cgctagagcc gcttacttct gcttccagtt ttgaaaaact gaaatcatag
    28621 ctttctaccc cggcagctgg tgttccttgc tgtatcccat aaagtcgtac cgacttgctt
    28681 ggcttgatcg aatcagctct cactgttact tgcactggat cagagacgat acttaatact
    28741 tcccctgctg cattcagcgc ctgtattggg acctcactat taatcccatc atctgtgacc
    28801 atcgaagcgt ccacatcagc tactactcgt ttgatttctg ccaatgtttt gtcacctgta
    28861 gtgatagaga cttttttagg actaacggaa gtatcatcaa gttcatatcc agatggtgtg
    28921 aacgttgaag gaatcaacgt ttccacatcg aaatctttta ttaccttttt ctcaaccgtt
    28981 actgtaatag tcgatggttc gatctttgct gtgacagcac tgctcaaatt ttgaattttt
    29041 agcggaacct catgtgttcc ttcgcccagc ttagtgatat ctgctgtgac gcggaacatc
    29101 cgtgtatctt cgtttgcctc tgcattcaac tgaactcggt ttgcactgga aagtttgaca
    29161 gtcactgcat tttcatatcc atggatatag tagcgctttg aatcataaga aatcttgatt
    29221 ggcaccccag taactgtttg attatacgct tcatttccag aaagcgtatt tggaacggac
    29281 tggccatttg cattgaagta cagcgcaata ctgaacagca atgcaataaa actgtaaaga
    29341 atgcttgtcc gtttctcttt tgagatcatt tgcgatcccc tcccttagtc ataccgtcta
    29401 ggaaagattg aatcacgttt ttcttgtttt ttgttttctc ttcagcaatc agttctctac
    29461 gcaaaattgc caaatattct tgctgggata agccagccat cagttcatta tctaatgtaa
    29521 tgctgacatc cccagtttct tcagagacaa cgatggtaat cgcgtcgctg acttcgctta
    29581 taccgacagc tgcccggtgt cgagtcccga attcttttgg aatcaacata ctttctgata
    29641 gaggaagata ggcgcttgct actgcgatct ttccttcttt tacaataact gcaccgtcat
    29701 gcagcggtgt attaggaata aagatattga tcagcagctc tccagtgata tctgcatcta
    29761 atgcaattcc tgtctcaata tactcatcta atccagtatg ccgttcgata gtaattagcg
    29821 caccgatttt tcgcttggac atatattgga tggctttgtc aaaagacaag atcatcttct
    29881 catcttcctg ctgttcgctt tttgattgtt tgaaaagcga actgcgtcca agatgttcca
    29941 gtcctctccg gatttccggc tgaaaaatca cgatagcagc tatcaccccg tacgtaatga
    30001 cttggttcat cagccaagac aaggtgtgca gaccaatgat ttccgctacg attcggataa
    30061 caataaaaat agctacacct ttcagaagct gaatcgcttt tgtgcctcgc agcagttgga
    30121 tcagtttgta tacaagatac cagacaacca gaatatcaag tatgttcacc aaaaaattca
    30181 gagagaacaa gtcaccggaa aacatttgct gccaatagtc caaattaaaa agttccccga
    30241 tttgaaatga cataaaaagc ctctcttctc ttttgagatt tcctctctag tatatcatat
    30301 gttttaattt tagaggctga tctgctcgta tttttcttga gaaaaacgca taattttcag
    30361 gaaaattgca agtatgcgtt ttatttttct ctgcaataaa tatttttaaa cttcagagaa
    30421 ttattcatta tattttttct gttatttttc ctttttttct tttgtagctt gttccatttt
    30481 tttacgtcgt tctgctctgg cagctcttct ttcttctgct tcaaggtccg tcgttttttc
    30541 ttttctagtc ttaacgggta catcgataga ttcctctggt tcaagtcgtt tcttgatttt
    30601 ttctaattct cccgtcagtg cagcatagtt gtaaaaacgt gttttagcgt cttggatagt
    30661 cttttcaaat aaagcttttg caagatttgg atttgcgttc tctagcgagg aaaagcgaac
    30721 ttgtttcctc atgaaatcag gcatagcaga gaaatcaggt tttttgtagt ccaaagtcat
    30781 tgggttcttg tttttttcga ccattgcagg gttatatcga tataatgacc aatagccaga
    30841 agaaacagct tcttttgctt ctttaagcga ttctttcatt ccaccaagca aaccatgtgt
    30901 gatacaaggc gtataggcaa tgattaaaga aggtccagga tacttttctg cttcttcaaa
    30961 agcctttatc gtctgcattt gattggcacc tgaagcaatt tgcgccacat aaacatttcc
    31021 atatgtcatc gccatcattc ctagatcttt ctttgaagca tactttccac tagccgaaaa
    31081 tttagcgatt gccgaagctg gtgttgcttt tgaggtttga ccgccagtat tggaatagac
    31141 ttcgttatcc aaaacaagaa tattcacgtc tgctccgctg gcaagtacat gatcgatccc
    31201 gccaaaacca atgtcgtaag cccatccgtc tccaccaatc atccattggc tgttttttac
    31261 gaatagatcc tttctcttgt atatatcctc cagttcaggg acaattgatc gttcgctttc
    31321 gatcgcagct tgcagttttg ctgctctttg ctgcgttcct tcgctctcaa acatgtgtac
    31381 caaccagtct tccatcaatt ctcgtagttc ttctgatact tttggcaata cttctttgat
    31441 tgaacttgcc aaagcctcgc gacgagtctg tgccgctaga agcataccat aaccaaattc
    31501 agcattatct tccaataaag aatttgacca tgcaggccct tgaccacatt cattggtcgt
    31561 ataaggagaa gcaggagaag caccgcccca aatagaagaa cagcctgtcg cattcgcgat
    31621 catcattcga tcaccgaaca tttgtgtcaa taattttaca taaggtgttt cgccacagcc
    31681 tgagcaagcg cccgagaatt caagaagcgg ttgattgaat tgtgtagaca agacagtatt
    31741 ctttcctttg actggatttg ctttttgtct taatgtcatt gcaaaagccc aattcacagc
    31801 ttgttctttc tgttcttcat aaggcttcat cactaaagct ttttcttttg cgggacaagc
    31861 ctctacacac agaccacaac ctgtacagtc ttccactgaa acttggatac ggtattttac
    31921 gccgtctgcg cctcgcattt ctctcacgat aaatccttct ggcgcttctt ccatttcctc
    31981 ttcatctgct aaaaatggac ggatggcagc atgaggacaa acaaaggcac attcattaca
    32041 catcgtacag cgatcactaa tccactcagg tacttctaaa gcgacgcccc gcttttccat
    32101 agaagccgtt cctaaaggca tttcgccagt tgtcattcca tttgctacaa gtgtaccaac
    32161 agagagttga tttccttcct gtgcattcac tggttcaagt atttcttgta tataaccagt
    32221 gttcctttta ttgatttttt caggcatctc caacgttctc caatggtctg ggatcgcgac
    32281 ttgatgaagc agttcggtcg tccgatcgat tgcttgtatg tttttttcta cgatagccat
    32341 tgatttatgt ccatagcttt tcagggcttc ttcttttagc agcggaagca cctcatcaaa
    32401 aggaatgatg tcagctaatt taaaaaaagc cgtttccatt gccgtattga tccggcgtcc
    32461 caaaccgact tcttgggcca atctcatggc attgatcgta tagaatctaa tgttattttc
    32521 agccaagtaa cgttttaatt ttaagggcag atgcgtttct aattgttcgt cgctccaaag
    32581 agtattcaat aaaaaaatgc cacctggctt caatcctttg accaagtcat agctatgcag
    32641 gtaagctggt gtgtgacagg caacaaggtc cgcatgctcg acaagatacg ctgaacgtat
    32701 cggcgtatct ccaaaacgaa gatgagagac agtcagaccg ccagattttt tcgaatcata
    32761 ataaaaatag ccttgcgcat atttatcggt gtgatctcca ataatcttga tagctgattt
    32821 atttgctcca actgttccgt cagatccaaa tccccaaaac ttggcctgga atgtttgcgg
    32881 ttctgtcaaa tccagacttt cctttggttc caaagattga taagtcacat catccacgat
    32941 ccctatggta aaccgttttt tgcgtatgct tgggtctttt ttcaattcat caaaaactgc
    33001 tgagatttgg tcgggagtca catctttaga ccccaatccg tagcgaccac caataacagc
    33061 cgggcgaagt tcagaatcat ataacgcact ttgaacgtct aataagagag gttctccacc
    33121 tgcccctggt tctttacttc gatcaagaac agcaattgtt tttactgtat gaggcaactt
    33181 ttgcacgaat gtttccactg gaaaaggacg ataaagatgg atattcaaaa aacctgtttt
    33241 ccgtccgttt gcattcaagt aatcaatcgt ttgttcaatc gtctgtgcaa ctgatcccat
    33301 agaaacaatg acttcttccg catcgtctgc tccgtaataa gtcaccaagt cataagaagt
    33361 tcctcgtaat cggttgatct ctttcatata tttttgaacg atttcaggta atttatcata
    33421 ataggggttg atcgtttccc gttgttgaaa atggatgtct ggattctggt tcgtaccact
    33481 aacagtcgga tgattgggat tcatccctcg tttacgaaat gaagctaatt tctcctgatt
    33541 gatcagagga gctaattctt catattccaa aacttcgatt ttttgaatct cgtgactcgt
    33601 acggaatcca tcaaagaaat tcaaaaaagg tacttctcct tcaatagaag caagatgggc
    33661 aacaggtgct aaatccatca cttcttgtac actgctttca gctaacatag caaatcctgt
    33721 ttgtcttgca gccatcacgt ctccgtgatc tccgaaaata ttcaacgcat ttgtcgtcac
    33781 tgcccgacta gcaacatgga aaacacttgg cagtagttcc ccagcaatct tgtacatatt
    33841 cggtatcatc aaaagcagcc cttgagaagc ggtatatgta gttgtcagtg caccagtttt
    33901 caatgatcca tggaccacac cagctgctcc tgcttcggat tgcatttcta ctacttttac
    33961 tggttgaccg aatacatttt cttttcctgc tgctgaccat tgatctacta attcagccat
    34021 ggtcgaactg ggtgttattg gatatattgc agcaatctcg gtaaatgcat aagaaatata
    34081 tgcggctgcc gtattgccat ccattgtttt catttttcgc atgattcatc atccttgttc
    34141 atattctctc ttattatctg ttccattata acgtaaaaca ttccattttt tcttttattt
    34201 ataaaaatat ttcacatatg tctatttgaa ttggtcatct aatttttaga caaacagtct
    34261 aaatcgttgc ctttttttgt cagacatgta taaactataa gcgaaagagg tgttttaaca
    34321 tggatatatc agtgattgat gcaacgaaaa caaatacaca aaaaggaatc ctttatggtt
    34381 catcgaatgc tcctaaaaaa atggtcgaat tcatcaacct atcttgtccg tattgccgac
    34441 aatggtttga agaatcttat gagctattag aagaagcagt ccaaagcggg caattgcagc
    34501 gagtgatcaa attgttcgat aaagaaaaag aaagcttgca gcgaggaaac gtgatgcacc
    34561 ggtatctcac aatcagcgat ggtcaaaaag cgatcaaaga gatcaaacaa atattcgata
    34621 ctcaagacga gtggaaacat ctttctctcc aagaagtagc ggattttgca gtagataaac
    34681 taaagctgac agaacaaaaa gacgaacagc tgagccaagc tgtcatcaac gaagctgagc
    34741 aagcacatat tcgatttgtt ccgactgtca tcttgggtaa agaaatcttt gatgaatcta
    34801 tttcaataga ggaattaaaa gaattgatcc aaacaaaata aaaaaataag gagcgtagga
    34861 caaaagtaaa gaacactttt gtctcacgct ctaaacacct ataaacggcg gaagcagaag
    34921 caactccttc ttatttcttg gagttaaacg cttctgtccc agcctcctta ttccgaataa
    34981 atggtgtcca aaagcttgca tctgccagat tatctcattt cttatgacaa gctgagcaaa
    35041 ttatctccac caaataattt attcatattt ttgacttgcg catactaact aattcttaaa
    35101 ttttcataca aaatttctca aattccctta acgagtaata tttatgtacg gtgattcaca
    35161 ctagtatccc aatcactttt taagttatcc cgatttcaat tctacttcaa aaatagttgc
    35221 ttttatgtat gctgttagtt gattagatgg tcgtttgttg agctaggtcg tttattttaa
    35281 acaagttgtt tctttagctt atacctatct cgtctaccaa acgcaagatg gcaaacacaa
    35341 atgcatacgc atctgtattt gccactttga ttcgtataaa gctaacaact cctaactcct
    35401 ctcatttaat agttgctctc ttttttattc agatacattt ttgctgatca cagcctagta
    35461 tttcaatggt tttgaacact aaaccaaatt gttccattac aacattcact ggctgatgaa
    35521 atggattaat agatttcgat tattcaaagc gattttctat tggtggattg tgttcccaac
    35581 taacaagacg gattccaaag tcattatata actcttcaag agttaaccct ttcctgttcc
    35641 caatagatgt atctctccca cgctcgatat aaaatatgga gtttcgaatg ccaagacctg
    35701 gcaaatcttt ccttgttaca gttacactgc tactaacttc cccatataaa atccagctcc
    35761 tccaaaatga taatatattt ttcgaccatc atctaactca tgtaacaata attgttgtgc
    35821 cctcttgtca ctcgccaaat gcaaacgcca cgtgcgatac catgccatgc tacctgcatc
    35881 tgcagaaaat tcatcaaata atgcaacaat tgtatcttct atcacaatcc tttccccatc
    35941 tacttcatat tctaatctaa aatcaaactc ccctcgggta atcgcaggtc ttggtggctt
    36001 aggacccaat attattctac ttaaaaccgc attcgttccc agatgaaaca cagctataac
    36061 aacaataatg attaatattt tgtttctatt tgataaattt ctcattattc gcaaatcacc
    36121 tctacattct cacatactag ttcgtaatac tcctttaata tcaaaatatc tactgatcgt
    36181 aataatattc gctatgaatt cctactactc gtataagaaa ggccaaatca tcagctttta
    36241 cttgctgatg atttggcttg tttctattta tcgtaattgt tttctaatca ccaaaattgt
    36301 taacaatcgg ttcagatatt tcccaattca ctagccttat attatattga tttaacaatt
    36361 cttcttcatt tattgtattt tgatcaagcg gaccaaattg ttctaataat tctttgtcat
    36421 gtaaaatatc ccttctagta atgcctcttt ccctttcaat gaaagaagca ctaggaaaag
    36481 tatgattata accattcaac ctatctccca tgtaataatt agcaggtcct ggaaaataaa
    36541 atatttgctt attatcacta acttctaaca gcaacactaa atcttctccg ctggctaaac
    36601 gacttgtcca tctacgatat ctccctatgc cttcgttcat tccaaatcca tcaaattcaa
    36661 ctattaacgt gtcttctatc acatgtaatt cttcattcaa ttcatactca agtctaaaag
    36721 gaaattctcc atatgtaatc tctggcaatg gtggtgctgg agttctccaa ataaatacaa
    36781 taactgtgaa tagaaaaaat ccaacgatcg atataaatgt tatgagacct gctcgaagta
    36841 aaacccgata taataaaaat gctgtatttc gctttgctat atctagaaaa ataaaaagta
    36901 ctgcaatcat aatgatagta aaagttgtaa aaaaacgcat ttttgccctc cctttataaa
    36961 catcgattct caaattctaa accaaatctt ggtagttcaa gttcaggtat aacgtattcc
    37021 aaccatacat ctcaattact atttatgaac ttatgattat cactgaataa atcactccat
    37081 ccccttgtta aaacttctac attaattaca cctggttgtt ttcgactagc taatccaatg
    37141 aacttatcaa ccatccttat ccaccaacca cgagaatctt gaccagaagt aagaagagca
    37201 gttctcaagg atgatcgagc agttccttcc agttttttaa ttaatcaggc cagttataga
    37261 taaatacttg atcttatcta tatcaggact tctatattaa gcaaaaattt atgtacaaaa
    37321 ctaaaatctt cattcagttg tctccccgct ttttatacga ttgtattaaa aacaatagaa
    37381 gcctttccaa attatgttac tccagtaaag atggaattat agtttgacgg aagaaaagca
    37441 agtataactt ttaaaaaaaa ctgatacatt cgcatctcct gcttcatgta tcagtttttt
    37501 ctttggatta aactgcttct tcacgcagtt tttccgcaaa ttcatttatt ttgcgaattc
    37561 ggtgattaat tcccgatttt gatatcgcac ctgagggaat catttcccct aattctttca
    37621 agctgacctc tggatgttga agccgaagtt ctgcgatttc ctgaagtttt tcaggaaggg
    37681 catgtagacc gacctttgct tcgatcaatt cgatgttttc gatttgttta gaagccgcat
    37741 caatcgtctt gttcagattt gctgtctcac aattgactag acgattgacc gaatttctca
    37801 tatcccgtac gattcgaaca tcttcaaatc ggagcattga gttggttgca ccaatcaatg
    37861 ttaagaaatc agcaattttt tctgctcctt ttaaataaga aatatagcca ttgcgccgtt
    37921 caagcgttcg agcatgtaga ccgtaataat ttaacatatc gcaaatgtct tggttatgct
    37981 gttcataaat agaaaatatt tccaaatgat aacggctcgt ttccggatta ttgactgaac
    38041 ctgaggccat aaaagcacca cgtaagtaag aacgcatttt ttgcgaattt cccataatct
    38101 cgtccgaaac gtgtccattg aacatcatac catccatgat atcaagatcc atcaacacat
    38161 cttttgtgcc ttgcttcaac cggacgatat agacattgtt cttttttaat ttcatttttt
    38221 tacgaacgag tagctcactt cgtacctgat aatgatcttt caaaagagaa tagatacgtc
    38281 tggcaattgc agcattttct gtttggacgt tcaaaataaa ctgctggttc acaataccta
    38341 aagagccgtt cattcgcagc aaagcagcta attccgcttt cgcatgttct cgatgaactt
    38401 ccaaaccagt tagttctttc ttcacatctg cagcaaatga catcctattc tcctcctttc
    38461 tccaacaata aattgcttaa tattttgaac cgaaaactaa acggaacaat tcttctacta
    38521 ctttgtctcc atcgtggaag acaccaccat ctttcaattc caaaaagtcg gtggaaatca
    38581 ctcgacatcc ttcatttctc aatccgttga agtcgtgtgc gacttgtact agatactcat
    38641 catagatctc tgggtccata taatcttcag gcactttttc cgtattgacc aatactgtat
    38701 cgataaaagg ctgattcaaa tgcttgtgta aaacacgtat atgatcggca tctgtgaaat
    38761 gttccgtttc ccctttttgg gtcatgatat tacagatata gacggtttca gcagttgtct
    38821 tgagcattgc ttcgccgatt tctggtatca ctaaatttgg aaggatactg gtgaataaac
    38881 ttccgggtcc caaaacgacc atgtcggcgt ccataattgc tgaaacgact tttcgagccg
    38941 ctttggcttc acttttatca tttgtgttgc agacatacac acggtcaatg gttttacggt
    39001 cgatcgcgat tttagattct cctactgcag tcgttccgtc tttaaagaca gcatgcaagg
    39061 tcaaaggagt ttctgaagaa ggataaacat gtccatctac atgcatcatt tttgctaata
    39121 attgaatcgc ctcataagtg cttccccgca tctcggaaac tgcggcaatg atcagattac
    39181 cgatcgtatg attggctaaa aactgatctt cctttttgaa acggtattgg aaaatatctt
    39241 catagaattg tggcatatcc gataacgcca ctaagacatt tctcaagtca ccaggaggag
    39301 ccatagcgat cgattctctg attgcaccgc tgcttccccc atcatctgca acagtcacta
    39361 ctgctgtgat atctgctcct tgatttcgca aacttttcag gataactggc agaccagtgc
    39421 caccgccgac cactacgatc tttggttttc ttatccggta ggttttcatt ttcacgaccg
    39481 gttcaccgtc tctttccttt tatccttgtc ccgatgtgtc acattgactt tatagttttt
    39541 ccctaaagca tttgccaaac gttcagtcaa tgccacagaa cggtgttgac cgcctgtaca
    39601 gccgattgca actgtcaagc tgctttttcc ttctttaatg tatccaggca atacagtttc
    39661 cagaagatcc aaaaatttat ggtagaatga ttgcgtttca tcaaaggaca tcacataatc
    39721 atagactggc tgatcttttc cggtcaatgg tcttagttcc gggatataat gagggttggg
    39781 caaaaaacgc acatccatca caatatccga atcgatcggc aacccgtatt taaaaccaaa
    39841 tgatacaaat tcgatccgaa atccttggtc tccctttgat ttgaatgact cattgatttt
    39901 ttcacgtaat tgtcgtggtg tcaatgtcgt cgtatcaatg atcacggaag cttgggcttt
    39961 caagtcgtct agtatcgccc gttctttacg tatcccttca gtgatcaaac cgtccatcgc
    40021 cattggatgt gctcgtcttg tttctttgta gcgagccact agttcttcgt ctgaagcatc
    40081 taaaaataag atagaagtgt cgatgaagtt cgtattttcg atttcgatca gcatgttttg
    40141 gatttcttca aagaaggaac gagaacgtaa atccactcct aaagccattt ttgtgacttt
    40201 gcctgattct ttgattaatt cccagaattt tggaatcaat cgaggcggca tattatcgat
    40261 acaaaaatag cccatatctt caaaactttg gatagctact gttttccctg caccgctcat
    40321 acctgttatg atgactaatt gcaaattatc tgtcataatt acgaactcct ctcacattgt
    40381 gcatagtaaa attatatcat tccgggcgta tcattaccag ttttcttttt cttttacaaa
    40441 aagatttgga ccaaatatct tttaggatat ttgccccaaa tccaattttt ttacttttcc
    40501 gatgctgtat ccaatgcctt gcttctttca gctagcgctc gttcaatcgt ccgatctgct
    40561 aaaatagatt gggcatcgat cactggataa tggttatctt cagcaaattc atgcatcaat
    40621 gacaattctg tgcaacctaa gataactttt tcacagttta ggcgctctac cgcttcttct
    40681 aagatttcgt aatatagctc ttgattcaaa taatccgatt ccttgatctc atgatagatc
    40741 agataattga ttttttcttg aagaacagta tcaggaatca ctgtttcgaa tccaagattt
    40801 ttgacttcgc gttcatagat tccagctttc atacttcctt ctgttcctag aatcgctact
    40861 ctccctgtcg tatgctgacg gacaagttca tttgctgctt ctcttggcat atgcaagata
    40921 ggaatatccg ttgccgcttg taactcttca aaaaagtagt gtgcggtgtt acaagtcaac
    40981 acaatgaagt ttggtcgcaa taaattttgt ttctcaatat catccagcag aaacggcatt
    41041 ggattttctt ctgatcgatc taaaatataa gctgtacgat caggaactgt tgcatgattg
    41101 aataagacat aattcaaata ttcttgatct tttgtagctt tcgttcggtg gttgatcaag
    41161 cgaacaaaac tttctgtcgc catcgtgccc attccgccta aaatactgaa aaaattctcc
    41221 atgaactagc cttctttcac atgaaaatac tttttgaatc ttggaatata gttatggaac
    41281 atatatttca tcaacaacca gcgcttgatt gatcggtctt tttcataaaa tacagtagta
    41341 ccgtaacgat tttctttgat catttgctca gctagttttt tgtcttcatt ttcccgagca
    41401 tattctaaaa agattttttt gggcacaccc atccataatc ttgctttatc tgattgattc
    41461 gttccatagg tcgtttcaac aaagggtttg ttaaacactc gatcttctgt gacgaaacga
    41521 gccagattca agccgttcaa tgtcacaaag aaactgctac gaccttgtct caaattgatt
    41581 tcaaataatt tatattctcc atctctagga tcatacttca tatcgaaatt ggcaaagcct
    41641 gtatattcga ttttttccaa aaatgcctta atggtttgat agattttttc gttataatct
    41701 ggcatgatca caacgtaatt accaatcgat gctggtgtag gatcttctaa cagtggatga
    41761 ccaagacaca tcatccgaac ttgatgatct tcatctacat acgcatttag cacacgcata
    41821 ttactatcat cacccgggat aaaatcttgt acaatcattt cacttttata accggcttcg
    41881 tatatccggc ctaaaattaa atcgaactct tctctagtct ctaaaataaa agcttttttt
    41941 cttccttcaa attggacaga aagatactcc acactgtttg caggtttcaa agcaactggg
    42001 aattcaaaag gcaattcctg ttccaaatgt ccatttacta acatttcttc tctgacgatc
    42061 aatgttttag gatacggaag atcatattct tcacaaactt cataaaagct tactttattg
    42121 atcagtcttt caaataaaga ataatcaata tatggacaga taaaatattc tgacagctct
    42181 tgtttatgtt gggagatcaa ttctgcatat ccatcaccac aagcaatcaa aagatatttt
    42241 ttactcttat cagaatatct ttcttttgcc aaacgaagca tcgtctcgat aaacacaggg
    42301 tctttatcaa atcctggaat aacttctaca ttgacaattt tactgtagcg ggtaggggct
    42361 aactgatccg aagcatatgc ttggcagatt ttgccgtatg cttcattaaa agaacgtgcc
    42421 ataccataaa cgttcatgtc gcttcctaat aatataggga taaattcttc attttcaata
    42481 ctgttcatca ttatctctca cttactcata atctcttgtt tttcttttcc aactacacaa
    42541 ggaatatcat accataaaga aaaaagcaat caaaggaaac tcctccgatt gctcaattct
    42601 tttttgaacg gattgaactt ataggaagtt tttgtctata ccaatcagtg tagaaagaac
    42661 taccatgtaa tgattctaga acactgatat taataaaaca agcaaaaatt cattttttta
    42721 tgttttttta actatctcag ctatctatcc tctttatacc attatttttt tcaaatagtg
    42781 acctgtataa ctttctttta cgttaatgat ttcttctggt gttccagtcg caaggatcgt
    42841 tcctccacct tctccacctt ctggtcctaa atcaatgata taatctgctg ttttgattac
    42901 atctagattg tgttcaatca ctaaaactgt gttaccagca tctactaatc tttgtaatac
    42961 atgcaacaag cgggcgatgt catcgctatg aagtcctgtc gttggttcat ctagtatata
    43021 gaaattcttt ccattagaga ttttgtgcaa ttcactggca agtttcatcc gttgtgcctc
    43081 accaccggac aatgtcgttg ctggttgccc catcgtcaca taacctaagc caacatcaac
    43141 aatcgtttgc agtttgcgat gaatctttgg aatgtgcttg aagaattcta cagcatcttc
    43201 tactgtcatt tccaaaatat cagcaatgct ttttcctttg taatgcactt ctaatgtttc
    43261 agagttatat cgtttgccat gacatacttc acaaggaaca tagacatcag gcaaaaagtg
    43321 catttcgatc ttaataattc catccccgcg acaagcttca caacgaccgc cttttacgtt
    43381 gaaactaaaa cgccctttct tataaccccg catttttgcc tcgttcgttt gagcaaataa
    43441 cccacggata tcatcaaata cacttgtata agtcgctgga ttacttctcg gcgtccggcc
    43501 gattgggctt tgatcgatat cgatgatctt ttcgatactt tcgtagccgg aaattgtctt
    43561 gaatttacct ggcttagcag aattcttatt taatttttgc gctaacgatt tctttaagat
    43621 actattgacc aatgtactct ttccagaacc agacacacct gtgactgcta caaattctcc
    43681 taatggaaat tcaacactaa tatttttcag attgttttct gaagcaccag tgatcttgat
    43741 tgctttccca ttacctttac ggcgttcttt tggtacaggg attgattttt tccctgaaag
    43801 atatttccct gtcaaagaat tttcatcatt agctacttct tcaggagtgc ctgcagccac
    43861 gatttgtccg ccttgttctc cggccccagg tccgacgtca atcagataat ctgctgccca
    43921 catcgtatct tcatcatgtt cgactacaac gagggtgttg ccaagatcac gcattttctt
    43981 taatgagcca atcagtcgat cgttgtcgcg ttgatgcaat ccgatcgatg gttcatctaa
    44041 aatatacaaa actccagaaa gattcgatcc aatttgtgta gccaatctga ttcgctgcgc
    44101 ttctccccca gataatgttc cggaagcgcg actcaaagtt agatagtcta gtcccacatt
    44161 ttccaagaat gaaagtctgt catttacttc ttttaaaatc ggtttggcaa ttactttctc
    44221 ttgttcagat aacgttaagt ttgagaagaa atctactgct ttttgaatag acaactcatt
    44281 tgtttgcccg atattcgttc cattgatttg aacagataga gcttggggat tcaatcgata
    44341 acctttacat gtctgacaag ttaattctgt catataaaga cgcatttgct cacgcgtgaa
    44401 atcactgttc gtatcatgat aacgacgctc gatattcttc aatacccctt cgaaagtcgt
    44461 ttctacatcg cgaacgccac caaaatcatt ttcataatgg aaatggaaaa gatcatctgt
    44521 cccattcaag atgatatcct gatgctcttc cggtaattct tcgaatggtg tatctaaatc
    44581 gataccgaaa ctttcacaag cctgcgctaa catttgagga taatactgag agctgattgg
    44641 attccatgga acgatagcac cttgactaag tgtcttcgtt ctatctggaa tcactaaatc
    44701 ttgatcgact tctaatttga cacctaaacc atcacagtct ggacaagctc caaaaggcgc
    44761 attgaatgag aacagtcttg gttctagttc gccaacagta aatccgcaat aaggacaggc
    44821 atagtgttcg ctaaacaaca tttcttcttc accgatcacg tcaaccagag catagccgtc
    44881 tgccaatcgc agagccgcct ctaaagaatc aaatagacga gaacgaatcc cttctttcac
    44941 aatgatccgg tcgatgacga tcgcgatatc atgttttttg tttttctcca gctcaggcac
    45001 ttcacttaaa tcatagatct caccatcgac tcgaacgcgg acgtatccct ctttttggat
    45061 tcgttcgaat atttttttat gttgtccttt tttctttacc acgatcggtg ccaacacttg
    45121 gatctttgtg cgctcaggca aagccattac ttgatctgcc atttgatcta ctgattgact
    45181 ggtaatctcg atatgatcgt tgggacaaat cggatgtcct acacgtgcaa acaacaagcg
    45241 taaatagtca ttgatttccg tcacagtacc tactgtcgac cgaggatttt tacttgttgt
    45301 tttctgatca atagaaatcg ccggactcaa tccgtcgatg ctatctacgt ccggcttatc
    45361 catttgtcct aaaaactgac gagcataagc agaaaggctc tctacgtatc tccgctgccc
    45421 ttcagcgtac agcgtatcaa atgctaggga actttttcca gaaccggaca aacctgtaac
    45481 gaccacgaat ttatctcgtg gaatcgttac atctatattt ttcaagttat gggcacgtgc
    45541 accgtgaatc gtaattttat cgttagccat gttgtcctcc tactttgaag cttttaattc
    45601 taaaatcata tctcgcacat tagcggctgt ttcaaaatcc aatgctttcg ccgcattttt
    45661 catttctttt tctaatttca tcaataacgt atctttttct tctttcgtca tttcttcata
    45721 cgagacttgt accgcttctt ttgtatcatc ttccgattct ttcgtaatag aaatcaaatc
    45781 tcggatttct ttaatgatcg tttttggaac gatcccatgt tcttcattgt acttctgctg
    45841 aatcgtacgg cgtcttgatg tttcatccat cgctagacgc atagaatctg ttactttgtc
    45901 tgcgtacatg attactttac cttcttcatt ccgagcagct cgtccaatcg tttggactaa
    45961 ggaacgttca ctacgtaaaa agccttcttt atccgcatcc aagatagcaa caagggaaac
    46021 ttcaggtacg tctaatcctt cacgaagcaa attgattcca actaacacat caaattcacc
    46081 taatcgaaga tctcgaatga tttctgttcg ttccagcgtt ttgatatcac tatgcaaata
    46141 tttgactttg atccctaatt ctttgaaata atctgtgagg tcttctgcca tttttttcgt
    46201 caatgtcgtt acaaacaccc gctggtcttt ttcgactcgt tcatttattt cgcctactag
    46261 atcatcgatt tgtcccatga ttggacgaac ttcgatcact gggtcaagca gccctgtcgg
    46321 acggatgatt tgttggataa cggtatctgt ttgttcatgt tcatatggtc ctggcgtcgc
    46381 agagacataa atgatctgat tgacatgttt ttcaaactct tctaaccgaa gtggccgatt
    46441 gtctaaagca gatggcaaac ggaaaccgta atctactagc atctgttttc tcgctcggtc
    46501 accattatac attcctctga cttgaggcat ggttacgtgc gattcgtccg ccacgatcaa
    46561 aaaatcttca ggaaagaaat ccaatagcgt ataaggcggt tcaccttctt tacgtccgtc
    46621 catatgccga gaataatttt caattcccga cgtgtagccc atttctaaca tcatttcaat
    46681 atcgtaattg gttcgctgtt ctaaacgttg agcttccaga agtttgtttt catttcgcaa
    46741 gacagttaaa cgctgttcta attccgcttt gatattcgct acggcatgct ccatatgttc
    46801 atcattcgtc acaaagtgag tcgccgggaa gattgccacg tgttccgttt ctcctaaaac
    46861 ttcacctgtc agtgcattga cttctcggat acgttcgacc tcatctccaa aaaactccac
    46921 acgtaaggca cgttcatctc ttgaagccgg aaaaatctcc acgacatcac cgcgcactcg
    46981 aaaacgaccg cgctgaaaat ctatatcatt tctttcgaat tggatactga ctaagtcacg
    47041 aatcaactga ttgcgatcta gttctgctcc ctgtcgaatc gaaactactt gtttttggta
    47101 ctcaaaaggc gaacctaatc caaaaataca tgaaacggat gcaatgacga tcacatcatt
    47161 tctttctaat aatgaactag tagccgaatg tcgtaatttg tcgatctcat catttacact
    47221 tgaatctttt tcgatatacg tatcgcttga aggcacataa gcttcaggct gataataatc
    47281 ataataagaa acaaaatatt caacagcatt gttagggaaa aattccttga attccccgta
    47341 cagctgacca gccaatgttt tgttatgggc aataattagt gttggtttat tcactttctc
    47401 aataagattg gaaatcgtat atgttttccc tgttccagtc gcacctaata agatttgtgc
    47461 ttttttcccg ccgactacgc catccactaa ttggttaatc gcttcaggct gatcaccagc
    47521 cggttggtat ttcgagacaa gctcaaagtg tcgggaggta tctctctcaa tcattcttcg
    47581 tcctcctttt cattcattcc ttttcatttt acgaccaata aaaagaaaag gaaagcaaag
    47641 gcattcctta ctaaaaaatt ataccacacc gaacatactt tcgccacttt tttatcttat
    47701 tatgcatgtt acgttttata acataaaatg ttagaaactc gcattttttt cattttttcg
    47761 agcttacagt cttgtctgat tcagcacttt tcgcctataa taataaaata ttggaaattt
    47821 aggaggttga ttatgaaaag aaagcattta tttcactcat ttgttttctt aatgacagga
    47881 cttgtgacac ttgcgtttgg acatgttgca caagctgaca acactacaac ggcagagtca
    47941 agcacaagcg agacaatgca aaaaatagag cccaaaaaag acgtatacgt aattgccagt
    48001 gactctgctt ttgctccatt tgaatttcaa aacgcaaagg gcgagtacga aggcatcgat
    48061 gtaaaattga tggaaaacat cgccaaactg caaggattta acatccgaat ggactaccgt
    48121 ggtttctctg ctgctttgca agctttggaa tcaggacaag cagacggaat gattgccgga
    48181 atgactgtaa cagatgaacg aaagaaaagc tatgattttt ctgattctta ctttgatagc
    48241 gggatccaat tagctgtcaa aaaaggagat acttcgatca agtcttatga agatttgaaa
    48301 ggaaaaacag tcggagcaaa agttggtacc gaaagtgctg aatttttaag cgaccatgaa
    48361 tctgaatatg gctattctat caaatatttt gatgctgctg atcaattgta cgacgggtta
    48421 aaagttggcg caatcgatgc aatgatggat gattatccgg taatcggtta tgcgatcaaa
    48481 caaggacaag aattggaaac accgatcaaa aaagaatctg gcggaaaata tgcatttgcc
    48541 gttaaaaaag gacaaaatcc tgagcttctt aatatgttca atgaaggatt gaaagaatta
    48601 aaaagaacag gcgaatacga taagattatt aaagaatatg tagcggatgg ttcagaaact
    48661 tccagtgagt cttccactgc tgatgaatca acatttcttg gacttatcca aaacaactat
    48721 caagcattgt tgaaaggact ttggagaaca attctcctaa ctttgatctc ctttgctttg
    48781 gctatagtaa tcggtgtgat ttttggatta ttcagtgtag caccaatacg cgctcttcgc
    48841 acaattgcta gtatttatgt cgatatcatc cgaggaattc caatgatggt tcttgcgttc
    48901 tttattttct ttgggttgcc tggtatcgtt ggatttacga ttcctgactt ccttgctggt
    48961 gttatcacct tgactcttaa cgcaagtgct tatatcgcag agattgttcg aggcgggatc
    49021 aacgccgttc ctgtcggaca aatggaagct tccagaagtt taggtctttc ttataatcgg
    49081 acaatgcaaa aaatcatttt gccgcaagcg atacgcatca tgatcccatc attcgtcaat
    49141 cagttcgtta tctcattaaa agacacaacg atcatttccg caatcggtgt agtcgaattg
    49201 ctgcaaactg gtaagatcat tgttgcacgt acaacacaaa gtacgtatgt ttacttgatc
    49261 attgcgatca tgtacttgat tttgatcaca gcattgacaa aattagcaaa agttttagaa
    49321 aagaaggtga agtaagatgg cagaaaaaat cttagttgag catttagtca aaaaatacgg
    49381 cgataacaca gtattaaacg acatcaatgt gtccatcaac gaaggagatg tcgtctgtat
    49441 cattggccct tcaggttcag gaaaaagtac atttttgcgt tgcttgaatc gtttagaaga
    49501 accttctgac ggtgaaatta ttattgacgg cgcccacttg atggataaaa gcacagatat
    49561 caacttggtg agacaacata tcggaatggt attccagcac tttaacctat tccctcatct
    49621 ttctattttg gaaaacatca ttttagcacc gatggacctg aagaaagaat ctagagaaga
    49681 agcagagaaa aaagcaatca aattgctgca gacagttggt ctagaagata aaaaagatat
    49741 gtatcctgaa atgctttccg gtggacaaaa gcagcgtgtg gctattgctc gtgctctagc
    49801 gatgaaccct gacatcatgc tttttgatga gcctacatct gcactcgacc ccgaaatggt
    49861 cggtgatgtc ttgaacgtca tgaaaaagtt agcagaacaa ggtatgacga tggttatcgt
    49921 tacgcacgaa atgggcttcg ctaaagaagt ggccaatcgt gtcatgttca ttgacgacgg
    49981 aaacttttta gaagacggaa cgcctcaaca aatctttgaa aatccgcaaa atgaacggac
    50041 aaaagatttc ttgaataaag tattaaatat ttaataaaaa ccaaaaatgc ccggtcgctt
    50101 catgacgacc gggcattttt agtttcttgt tttttcactt aactgtacca gccgcatcgc
    50161 ttgttctcct gcacaaacaa gcaatcgctc tacctgttcc atcgcataag aaagtaccat
    50221 cggttcattg acgatatcaa gtacatagtc gataccacct tcagaaacta tgatgattat
    50281 cttattctaa agctggcttt tttgttggta caaacatgac aactacaaaa aaaccaggcg
    50341 aacgtatgat tgttcgtctg gtttttaatt attaatgact tagcgttttt actagaaata
    50401 gaaaatttgg gcaagtctac tttatatttt tactagaaaa actacttccg ctttttcccg
    50461 ccaaaagcat ctttaaactt atcgaagaaa ccgtcttctt gctgttcttc aactttgatg
    50521 ccgccagctt cagcgaatgc acgtaatgct tctctttgtt gttcatttag atttttcggt
    50581 gtgatcagtt ttactttgac atgttggtcg cctgttgcac caccacgtaa cttcggagcg
    50641 ccttttccac gtaaacggaa gttcgtacct gtctgtgttc cagcagggat cttcaatttc
    50701 acatctccat ggacagttgg tacttggact tcatccccca aagctgcttg tacaaaactt
    50761 aaaggcaatt catagtaaat ttcagaacct tcacgatcaa agatatcact ttcttctaca
    50821 cgaaaaacca catacaaatc accaaatggt ccgccgtttt ctccagcttc tccttgacca
    50881 gctaaacgca tttgttgtcc ttcttctaca ccagctggta cattcacttt aactgaatgt
    50941 gctttcttct catgtcctga gccatgacag gttggacaag gttctttgat ttctttccct
    51001 gtgccatgac aaacatcaca agtttgacgg ctcatcactc gtccaagtgg cgtttggcgt
    51061 tctacattga tcgtacctgc accatgacat ttatgacaag tagttggttg tgtgcctggt
    51121 tttgcaccgt ttccaccaca agtatgacag atttcttccc gattatactt gatttctttt
    51181 tctacaccaa aaatcgcttc ttcaaaagac aagtcgaccg tatattgcaa atcagcacct
    51241 tgacgaggcg catttggatc gactgaccga ccgcctcctc caaagaaaga gtcgaagata
    51301 tcttcaaaac caccgaagcc gccaccagag aagccgccga atccaccaaa tccgccacca
    51361 gctccaccag caccgtaatt tggatctgtg cctgcatgcc cgtattggtc gtatgcagcg
    51421 cgtttttgcg ggtcacttaa aatctcatat gcttctgata cttctttgaa tttttcttcc
    51481 gcatcaggtt ctttattgat gtcaggatgg tattttttcg aaagcttacg ataagctttc
    51541 tttatttcat cttctgatgc tccttttgag agccctaaga cttcataata atcacgtttt
    51601 gttgccatag gctttcctcc tattggttct catcattttc acgtcgcttg ttatcatatc
    51661 atattttgtt tgaaacaaaa accgatactt caaactattt ttcaaaagaa aaagccaaag
    51721 tagcaacttt ggccttctct cgaaattcag tgaactgatt atttatcgtc gccatcgact
    51781 tcttcaaaat cagcatcaac gacatcatct gctccgcctt gagctgcttc tggattttct
    51841 tgagcttgtt gttgtgcagc ttgttcgtat agtttaacag tcaagttttg aacgatttcg
    51901 ttcaatgcat cgcgtttagt tttcatttcc tcgatgttgt ttgcttcaac agcggctttt
    51961 aattcgtcac gagctgtttc tgcttttttc acttcttctt cgtccacttt gccttctaat
    52021 tctttcaatg ttttgtccac agagaatagc aatgcgtcta catcattacg taggtcaact
    52081 tcctctttac gagctttatc tgcttcagcg tttgcttctg catctttcac catacgttcg
    52141 atttcttcgt ctgttagacc tgaagaagat ttgatcgtga ttttttgttc tttttgtgta
    52201 cctagatctt tcgcagatac atttacaatc ccgtttttat caatatcgaa tgttacttcg
    52261 atttgtggaa taccacgagg agctgctgga atatctgtca attggaatct tcctagagtt
    52321 ttgttgtcag cagccattgg gcgttcacct tgtaatacat ggatgtctac tgctggttga
    52381 ttatctgccg cagttgagaa tacttgtgat ttacttgttg gaatcgttgt attgcggtca
    52441 atcaatttag tgaacactcc acccattgtt tcgataccta atgacaatgg tgtaacgtca
    52501 agtaaaacaa catctttcac atcaccagtg atcacgccac cttggatagc agcacccata
    52561 gcaactactt catcagggtt aactgatttg tttggttctt ttcctgtttc tttacgtact
    52621 gcttctacaa ctgcagggat acgagtagat ccaccgacta aaataacttc gtcgatttct
    52681 gattgtgaca gacctgcatc ttttaacgct tgacgtactg gtacttttgt acgttctact
    52741 aagtcagctg ttaattcatc aaattttgca cgtgtcaatg tcatttccaa atgcaatggt
    52801 ccagattctc ctgctgtgat aaatggcaag ctgatttgtg tgcttgatac gccagataaa
    52861 tcttttttcg ctttttcagc agcgtctttc aagcgttgta aagccatttt gtcttgagac
    52921 aagtcaatgc tgttttcttt tttgaattct gctaccatgt agtcgatgat tttgttatca
    52981 aagtcgtccc cacctagatg gttgtcgcca gctgttgaca atacatcaaa gacaccgtct
    53041 cctagttcaa ggatagatac gtcaaaagta ccgccaccaa ggtcgaatac taagattttt
    53101 tcgtcgcgat ctgttttatc taaaccatag gcaagagcag ctgcagttgg ttcattgacg
    53161 atacgttcta cttctagacc agcgatctta ccagcatctt ttgttgcttg acgttgtgca
    53221 tcattaaagt aagctggcac agtgataact gctttttcga ctttttcacc taaatagtct
    53281 tctgcgaatc ctttgatata ttgaaggatc attgctgaga tttcttgtgg tgtatatgat
    53341 ttaccttcta catctacttt gtaacctgct tcaccgatat ggcgtttgat tgatgaaatc
    53401 gtatttgggt ttgtcacagc ttgacgtttt gcaacttccc ctacttggat ttctccattt
    53461 ttgaatgata caacagaagg tgttgtacgg tttccttctg ggttagcgat gatttttgct
    53521 tcgtttcctt ccaatactgc tactgcagag ttagtcgtac ctaagtcaat accgataatt
    53581 ttactcataa ttaacgtctc cttctttaat tgaaagtgta agttttatat ccgcacaatt
    53641 attgtgcaac aatgaccata gttggtctta acacgcgatc atgtaatttg tagccttctt
    53701 gtagtacttc tacaatcgta tcggctggtg tatcttcagt cgctggaaca gtttgtactg
    53761 cttgatgaag attaggatca aaggcttctc ctttagcagg gattttttca attccttctt
    53821 cttccagtgc gttacgcaaa ctttccaaga ccatttccac gccttttttc aaacctgccc
    53881 cttgatcatc tgacacttct gtggctaatg cacgttccag gttgtcaatt gatggaagta
    53941 attttttggc taaatcttgt gaacggtatt tttgtaattg ctcacgctca ttttttcctc
    54001 gatttgccat atttgcgatt tcagcacgag cccgcaagta tttatcttcc atttcttcta
    54061 actcagcttt taatttcgct gtttcgaatt cttccgcttc agcttctgaa atacccgaat
    54121 cttctgcagc ttcgacatcg atttccggtt ctggctgagc atctgtcatt tctttatcta
    54181 gttcttcttg attttctttc acgatacagt cttcctttca ttattcagtc agctgtttga
    54241 ttgctcttac taagaaagag agaacgatca gctgcaaata gcttcgttat tcacattacg
    54301 agtttgacaa gtcaagcgag cggtagtaat cagcaagttt agctgctagt tctcttcgat
    54361 aaacatcgac tagaccgaac atccgagaat aaggcatact agtaggccct aacaaggcta
    54421 ttgtacctct tccatgacca tctatctcgt aacttgcttg aatcatactc atattctgca
    54481 ataaatcatt gcctaattca gcaccaatct tgatatgaat attgctatcc atcggagtga
    54541 tcatttgtgt aagtccgtct gagtctttca taaaagaaaa catcgatttg aactgatcaa
    54601 gatcttgacg gggctcataa tccaagagat tcatttgtcc actgacataa accttttctt
    54661 caaaaacatg accaagaacg gtgtcaaata actgagacat cccttctgtc gtctggaaat
    54721 atttatgaag aatcatcgga atctccgtac gaagtttatg gtacaccgtc atcagaggat
    54781 ctccgactag acggtcgttt acaatctgta ccattttttc tagttcctga cttcccatat
    54841 attcgggaag agtgaacact tggctttcta cattccccat atctgttacg acgatagcaa
    54901 taatctgtcg attattcaag ggtacgatac gaaagccagt cagtcggcgg tcttttattt
    54961 caggtcccaa tgaaaaagtc gtatagcttg tcagctcaga taatatctca gcagattgct
    55021 taataatctc attgatttca tgaaattctc tgcctagaga ctgacgaatc agcagcaaat
    55081 catcttctgc tacgcgagca ggttttaaca aatgatccac gtaataacga tagcctaaag
    55141 cagatggaat ccgcccagaa gaagagtgag tcttcaacag caatccttca tcttctaatg
    55201 ctttcatatc attgcgaatc gttgctgagc tggccttgac tccctcagac attaatgttt
    55261 tggaaccaac gggcacgcca ctactcgtat agtgttggat gatcagacgt aaaatgtcgt
    55321 tttgtcgctt tgttaacatc ttcctcacct ctcattagca ctcatatatt ctaagtgcta
    55381 attcacttat aaatatagca agaagccctt ctcctgtcaa gagaaagact ccatttttta
    55441 gcactctaaa cacaagagtg ctaattctaa ataattgatg taaatccagt tatatcaata
    55501 cttcaatacc tcattgtcaa actttctaaa ctttttcttt tcaagtttcg ataaaaaaca
    55561 aatattcttc aaactatttt tcaaataaaa agcgctcaaa cacattgttc cccaaaaaag
    55621 tccctcttgg tgtcaaataa acacggtctt cttcattaac aagtaaccct tcttgctcta
    55681 attcccttag cacatcaccg tacaccgatt caattgtttt ttgatagcga tcatagaaat
    55741 gttttttagt aatccctgtt ttttttctta gtcctaagaa catttcttct tctatttgat
    55801 tttttcgtgt caatttttct tcttccaata ccgggcgttc atttgctcgt aatgggcgta
    55861 aatagtgttg gattggtcct ttgttgcggt aacgaatatc tccaagataa ccgctagcac
    55921 cagctccaaa cccaaaatag tggtcattgt tccagtacat gaggttatgt tgagattcat
    55981 gtccggagag cccaaagtta cttatttcat attggtgacg tccatgcttt tccattgctt
    56041 caatcgtctc ttcaaacatt ctggtttccg tttcctgatc aggcagttcg agtcttcctt
    56101 gtcgtaccca gttcatgaac attgttttgt tttcgaggat caatgagtac aaagaatagt
    56161 gaggcagatc caaagccaaa gctttttcca aagtatcccg atatccttct agtgtttgtc
    56221 ccggtaatgc ataaatcaaa tcgatgctta cattggtaaa attttctgct tctaaaaatt
    56281 gcattgtttg atacacatct tctgcagtat gtttacgtcc aatttttttc aataagcgat
    56341 tgtcaaatgt ctgcactccc atggataaac gattgacgcc atagtttttc atcacttgaa
    56401 gtttttcacg ggtcaaatca ccaggattcg cttctacagt aaactcatta ccattttgaa
    56461 aaggcaggat ctctctagca cctgataata gtcgatcaag ttgtttagca gataaagaag
    56521 ttggtgttcc gcctcctaca taaagtgttt ccaaattcgc tgtaggatga ctttccagca
    56581 ttaatctcat ttcattcagc agcatttcca catactcatc tactggctgc ccttctaaaa
    56641 aaactttatt aaagtcacag taatagcaga tatgctcgca aaatgggata tgaatgtacg
    56701 cagaaacctt gttttctgcg ggatttattg tttctatcat tgaaattctt tctccttacg
    56761 tcgaaaagtc gtgcctgttt tcgaccttct taaaatcgcg ttcaagcacc aactcattga
    56821 aataagccgc atatttagaa tcaaggaaac ctagctctaa aaatgcttgc ttattgtcat
    56881 cggagctaca cggtgctttt acagcctctc gataaacggt aatcgcaaag ctgaccttcg
    56941 gcattaagtc aaaatctgca aaaatatgaa aagctatttt cacagattcc tccttaagtg
    57001 ctcagaccac aacgctttgc ttacaacctc tcatatacgc atactaaacg tatttggcga
    57061 acaaatccac taaaaaatcc aagaatcgac ttcttggatg atttttttac actcatgatt
    57121 cttcctaaac aattttgtcc ttattttatt cataaaaaaa tctagtcaaa tctctcactt
    57181 caagagtttt aactagattc caattattat ttttttgtaa aaataaacag tctcttaatc
    57241 atagacattt cttcggtgcc ctacttgaat agcgataaca acgaattgat tatcttctat
    57301 ctctaccaat acacgtattt gccaatcaga gctgcttctg aatttcgggc gtgaatttta
    57361 aacggcaggt cattccagcc caccaaattc attaaggatt cttccatgaa tactgttgtt
    57421 ttcccacttc ttatccaatg tttatgggca atttcagccg tttgacgatt gtattcatct
    57481 tctagttgtt ccatcgaata gttttttaga agttcagata aagtaatccc ataaaattca
    57541 gccatataag aaagccattc ttttttggcg tcacttacac gaaccgtaat catagcccta
    57601 taattgcctc tcttaaagaa tctcatgcat ttcctgatgt gattttagaa aaatttactt
    57661 gctaaaaaac tgtgaaaatg taggagctag catccttgta gtaaatgatc gagctcttta
    57721 ttatacatta aaaaagttca gaaattttga tgaagtagcc aactactttt tcatgagtaa
    57781 agcaacacta gaaaaagaat cctccagtac ttagaattta ctggaggatg tacttttgaa
    57841 gaagcctata ggttatttaa aaaatattac tacaggaagg aacgagaatt acttcgttac
    57901 ttataataga acttagtctt atatagaagg catagctcac attagcttat ttgtcctata
    57961 aaaataaatt acggtatttt agaaaagcta ttttctcttt tttcaacgat cttcagcaat
    58021 ttttctttat cgtcaggtaa ctcaagttca tgaattctag catgacaatt gggacataac
    58081 gcgatacagt tttcaatcac atcttttcca ccttgcgaca aatattctat atgatgacaa
    58141 tgtaaataag gtacaccgtc ttttataaat ggcccttttt cttggcaaag ttgacaaata
    58201 ccttttgcaa gtcgtttaac atattcagat acgaagggat tacgtttata tatttttgtt
    58261 tttgagaagc aaaactcctc ttcgttaaca tattttgcag ccattttttt attataatta
    58321 ctaacttttc ttgcttcttc ctctaattta gcattagata aattacgaat atgtctagtt
    58381 tgattttctt cctggttata tatctttatc ttcaatcaat tgctcttgat ttattaaagc
    58441 taatggaaat ttatatactt ttctgctgtt gcccgaacta tctttttcat taacagtata
    58501 tatttgtcct gctaaatata cttctcccag atatgtgtgt tcttttggtt taaatgtttc
    58561 aaataaatga atctttacaa aattactttt tgaattggct aaaactttat tttgattctt
    58621 atccaatact tgatcccctt ttagccccat accggtatat tgtaaaatac catttaacca
    58681 cttatcttta taaacattac ttcctgtttg atcactaaac agtacaagag tattagtttg
    58741 atgagatctt ctcattccac cctgtttgga acatttaaac ttctctgaaa tttctttgtt
    58801 agtatatact ctaccaactt ctaactcacc taatatgttt ttagaccaat tttctcgcca
    58861 tgtaataatc ttatcaagat taaacatttc tccctctttt ggcatgccat ttctaattaa
    58921 attagctaat acaccagtag aaatatctaa tttttttagt aattctttct tagtaatcaa
    58981 tgttggattc ataacaatgc agctcctaat gatatcaata taattgcatt agataacttg
    59041 aaaaatcgaa aaaaactcat tttgtgtcaa gttatctgta atatataaaa atatcattat
    59101 tatacctcat ctatgtgaat ttttactatt cttaccttag catattcttt ataggtatat
    59161 cagagctaat caaactaaaa aatacccgaa gtcttcttat gtaaaaacac cggttattac
    59221 ttaaatatga cttaggtaaa acaatgagtg atccaattat aaaattttat ggcgaataat
    59281 ttagtaagcg ctacatattg aatgatttag aaattttgaa aaagtagcta gttcctttta
    59341 taccagaaaa gtaaacctaa taaaaaacac aactaatttt tagctgtgtt ttttaagtta
    59401 caataattat ctatttaata gtcttaattc attaaaataa aaaactcaac tttacaagga
    59461 tatggtttaa aaaatttatt tatccaaccc aactagtttt cgctgcttac gatttgcttc
    59521 atctgctacg taattcatta ctgttttaaa ttctttcgct gaggcgtttt ctgataaatc
    59581 agcgatactt ttattatact tcttcacgat ttgttgtact tgttctttct cagtcatgta
    59641 attcctcctc caactctttt gaagtatatt gtattggtag tactaattga atattatctg
    59701 ctgcataatg cacttcagaa atgtcgtttt gataatcctt aatcattatg tgaaggacta
    59761 cttcaggatg tctatttttg actttttcaa tattctctct ggacttaaaa aacgcattga
    59821 taaacatttc ttttggcaca tgacgtcctt cagctagttc acgttttcga gtgaaatccc
    59881 aggcaacttt gggatcttgg taaacataga aaatcgtaac gcgaaagtta tttttaaggg
    59941 ctcgaataat atttttctct gcactaagga tagcaaaagt atcctctagt atgaaagaat
    60001 agccatgttc cagtacatat tttaatgatt gttcaactaa ccaagaagaa gctttctgaa
    60061 aaacacttga gttctttcca ttgtattctg gaaattgtgc tctaaaatag tccgcatcaa
    60121 tcacaacatg gttgtcataa ttttaagcta aacctagtgc aacctctgtt ttacctgctc
    60181 ctgggcttcc agccatgaat accgcatctt tttcatcatc agggattttt cccttgatga
    60241 ttgtttcaat aaatttatct ttattgaccc tagcgtaatc taaatacttg tctttaggtt
    60301 ccaagattgc accttctttc cttcttctat attcatacta attatacgat atttatataa
    60361 aaaatataac ggtcatttta accatattaa catttagtca cagtaatagc agatatgctc
    60421 gcaaaatgag atatgaatgt acgcagaatc cttgttttct acggcattcg cttttttttc
    60481 attggactag ttatatattt ctagggttgt tttcatgtct gagtgcccga tatgatttat
    60541 ttttcgtttc tataattact ttttgccttc cagtttcttt taagattgta ctcaagcaag
    60601 tgggaattgg ttcttttgtc atccatgtat gcaaaatgtt ataatgctgg taaacaaaaa
    60661 tgattacctg tcatttttat ttagttcgta tttaattaag ttgactgtct gtatactctg
    60721 tctaaaactt aagaattgtt gttttcgctg tcgctatcca aatttaatga atgtctgtat
    60781 gttaaaactg ttgtccttgt catagatagt aaaggagagc ttgaaaatga aaactgcgat
    60841 tacgtttcta ttagaggaat atgccgattg ggaaggtgct tatattacta gcacactaaa
    60901 ccaaaaagag ggatggcaaa acaaaactgc ttctatccaa tcagaggtca cttccattgg
    60961 aggattaaaa acaaaaatcg attacttaat cgaacaaata cctgaaaaag ttgacctttt
    61021 tattatgatc ggtggcaatt catgggacat aaaaaatcat catttatatc aaataatcga
    61081 taaattgtta aaaacagacg taccagtagc tgctatttgt ggggcagttg attatttagc
    61141 tcagagcgga ttattgcaag gttacaaaca caccggaaat gcgcaatttc cttggttaga
    61201 atcggaaaat tatcagaata aagaagattt tatagaacaa caagctgtga aagatcgtaa
    61261 tttgattact gcgaatggaa cagctcctat agaattcaca gacttgattc ttcaagcagt
    61321 ccattttgaa acagaagaaa taagagaaaa aaagattcgt ctatacaaac ttggattcta
    61381 tgattactat aaaaagtacg gagatccttt ctcctaatca ttctcttcaa ttaaatactc
    61441 cttatcaata gtatgacaag taattttact gatggttatt tgtcacactt tttctgacat
    61501 ttatcaaaga agatacactc tatttatatt catagatgga atggcaacag tttttttgac
    61561 aaattttata aggtgca
//
LOCUS       DACGLW010000006        56389 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.006, whole genome shotgun sequence.
ACCESSION   DACGLW010000006 DACGLW010000000
VERSION     DACGLW010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 56389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56389
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.006"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            330..1175
                     /locus_tag="IXT53_000461"
     CDS             330..1175
                     /locus_tag="IXT53_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ4904316.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1456..2676
                     /locus_tag="IXT53_000462"
     CDS             1456..2676
                     /locus_tag="IXT53_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HAQ4904317.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2673..5393
                     /locus_tag="IXT53_000463"
     CDS             2673..5393
                     /locus_tag="IXT53_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ4904318.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5377..6321
                     /locus_tag="IXT53_000464"
     CDS             5377..6321
                     /locus_tag="IXT53_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ4904319.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(6390..7400)
                     /locus_tag="IXT53_000465"
     CDS             complement(6390..7400)
                     /locus_tag="IXT53_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ4904320.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(7580..7924)
                     /locus_tag="IXT53_000466"
     CDS             complement(7580..7924)
                     /locus_tag="IXT53_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ4904321.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7917..8351)
                     /locus_tag="IXT53_000467"
     CDS             complement(7917..8351)
                     /locus_tag="IXT53_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HAQ4904322.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            8489..9226
                     /locus_tag="IXT53_000468"
     CDS             8489..9226
                     /locus_tag="IXT53_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4904323.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            9219..10430
                     /locus_tag="IXT53_000469"
     CDS             9219..10430
                     /locus_tag="IXT53_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ4904324.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            10637..11416
                     /locus_tag="IXT53_000470"
     CDS             10637..11416
                     /locus_tag="IXT53_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HAQ4904325.1"
                     /translation="MEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAAL
                     SREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLKT
                     MLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHGD
                     VNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDEM
                     LEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            11486..12148
                     /gene="trmB"
                     /locus_tag="IXT53_000471"
     CDS             11486..12148
                     /gene="trmB"
                     /locus_tag="IXT53_000471"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HAQ4904326.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(12246..14000)
                     /gene="ade"
                     /locus_tag="IXT53_000472"
     CDS             complement(12246..14000)
                     /gene="ade"
                     /locus_tag="IXT53_000472"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HAQ4904327.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            14132..15061
                     /gene="cpaF"
                     /locus_tag="IXT53_000473"
     CDS             14132..15061
                     /gene="cpaF"
                     /locus_tag="IXT53_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component"
                     /protein_id="HAQ4904328.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            15018..16067
                     /locus_tag="IXT53_000474"
     CDS             15018..16067
                     /locus_tag="IXT53_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HAQ4904329.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            16064..16342
                     /locus_tag="IXT53_000475"
     CDS             16064..16342
                     /locus_tag="IXT53_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HAQ4904330.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            16317..16787
                     /locus_tag="IXT53_000476"
     CDS             16317..16787
                     /locus_tag="IXT53_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HAQ4904331.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16804..17106
                     /locus_tag="IXT53_000477"
     CDS             16804..17106
                     /locus_tag="IXT53_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4904332.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            17123..17494
                     /locus_tag="IXT53_000478"
     CDS             17123..17494
                     /locus_tag="IXT53_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HAQ4904333.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            17466..17825
                     /locus_tag="IXT53_000479"
     CDS             17466..17825
                     /locus_tag="IXT53_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4904334.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17997..19004
                     /locus_tag="IXT53_000480"
     CDS             17997..19004
                     /locus_tag="IXT53_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ4904335.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            19029..20213
                     /locus_tag="IXT53_000481"
     CDS             19029..20213
                     /locus_tag="IXT53_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HAQ4904336.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(20650..22233)
                     /locus_tag="IXT53_000482"
     CDS             complement(20650..22233)
                     /locus_tag="IXT53_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ4904337.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22811..22954)
                     /locus_tag="IXT53_000483"
     CDS             complement(22811..22954)
                     /locus_tag="IXT53_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HAQ4904338.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(23177..23656)
                     /locus_tag="IXT53_000484"
     CDS             complement(23177..23656)
                     /locus_tag="IXT53_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ4904339.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(23729..24013)
                     /locus_tag="IXT53_000485"
     CDS             complement(23729..24013)
                     /locus_tag="IXT53_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4904340.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(24091..24285)
                     /gene="ssrS"
                     /locus_tag="IXT53_000486"
     ncRNA           complement(24091..24285)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="IXT53_000486"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24357..25643)
                     /locus_tag="IXT53_000487"
     CDS             complement(24357..25643)
                     /locus_tag="IXT53_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HAQ4904341.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25781..26452
                     /locus_tag="IXT53_000488"
     CDS             25781..26452
                     /locus_tag="IXT53_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HAQ4904342.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            26418..26906
                     /locus_tag="IXT53_000489"
     CDS             26418..26906
                     /locus_tag="IXT53_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HAQ4904343.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26919..27866
                     /gene="prmA"
                     /locus_tag="IXT53_000490"
     CDS             26919..27866
                     /gene="prmA"
                     /locus_tag="IXT53_000490"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HAQ4904344.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27867..28631
                     /locus_tag="IXT53_000491"
     CDS             27867..28631
                     /locus_tag="IXT53_000491"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HAQ4904345.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28864..31077
                     /locus_tag="IXT53_000492"
     CDS             28864..31077
                     /locus_tag="IXT53_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HAQ4904346.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            31088..31534
                     /locus_tag="IXT53_000493"
     CDS             31088..31534
                     /locus_tag="IXT53_000493"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HAQ4904347.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            31705..32964
                     /locus_tag="IXT53_000494"
     CDS             31705..32964
                     /locus_tag="IXT53_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HAQ4904348.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(33053..34000)
                     /locus_tag="IXT53_000495"
     CDS             complement(33053..34000)
                     /locus_tag="IXT53_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAQ4904349.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            34394..35686
                     /locus_tag="IXT53_000496"
     CDS             34394..35686
                     /locus_tag="IXT53_000496"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HAQ4904350.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            35701..37470
                     /gene="aspS"
                     /locus_tag="IXT53_000497"
     CDS             35701..37470
                     /gene="aspS"
                     /locus_tag="IXT53_000497"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HAQ4904351.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            37579..38454
                     /locus_tag="IXT53_000498"
     CDS             37579..38454
                     /locus_tag="IXT53_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ4904352.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            38720..40603
                     /locus_tag="IXT53_000499"
     CDS             38720..40603
                     /locus_tag="IXT53_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HAQ4904353.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(40678..40962)
                     /locus_tag="IXT53_000500"
     CDS             complement(40678..40962)
                     /locus_tag="IXT53_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAQ4904354.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40952..41686)
                     /locus_tag="IXT53_000501"
     CDS             complement(40952..41686)
                     /locus_tag="IXT53_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HAQ4904355.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41815..42438
                     /locus_tag="IXT53_000502"
     CDS             41815..42438
                     /locus_tag="IXT53_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HAQ4904356.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            42630..43751
                     /locus_tag="IXT53_000503"
     CDS             42630..43751
                     /locus_tag="IXT53_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HAQ4904357.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            43758..46886
                     /locus_tag="IXT53_000504"
     CDS             43758..46886
                     /locus_tag="IXT53_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HAQ4904358.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46905..50099
                     /locus_tag="IXT53_000505"
     CDS             46905..50099
                     /locus_tag="IXT53_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ4904359.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(50245..50952)
                     /locus_tag="IXT53_000506"
     CDS             complement(50245..50952)
                     /locus_tag="IXT53_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ4904360.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
     gene            51717..52115
                     /gene="spxA"
                     /locus_tag="IXT53_000507"
     CDS             51717..52115
                     /gene="spxA"
                     /locus_tag="IXT53_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HAQ4904361.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
     gene            52447..53100
                     /locus_tag="IXT53_000508"
     CDS             52447..53100
                     /locus_tag="IXT53_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HAQ4904362.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            53326..54324
                     /locus_tag="IXT53_000509"
     CDS             53326..54324
                     /locus_tag="IXT53_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HAQ4904363.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            54500..56308
                     /gene="pepF"
                     /locus_tag="IXT53_000510"
     CDS             54500..56308
                     /gene="pepF"
                     /locus_tag="IXT53_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HAQ4904364.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
BASE COUNT        18554 a         9728 c        11687 g        16420 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tctttttttg tttaaatctt ttgctaagtt
       61 gaactaatag agtcttttat gaatatcgaa gttagttttt cacctaactt cgttttttat
      121 agttggttat tataaaatag taatatagct gatttggatt caattcatat ttatttataa
      181 atcaaaaata cagagaatag tatataaatg tcctttccta ttcttaaaat gatgtttgta
      241 cttacttaaa ttaggtataa ttagtatatg ttttgtgaga aatttagact tacaataagc
      301 agtgaattta taatcaacgt ataggagaca tggatgaaat gaaactaatt ggtatagatt
      361 tagacggtac tcttttgaat tctgaccaga gcattagtca aaaaaatgca agaacaatga
      421 aacatttacc ggaagactgt tttccattca tttgttctgg aagagaagta gaagacatta
      481 ataatattct caaaaaatct ggtttaagga taccagcagt agggttaaat ggttctgttg
      541 gatttgatgg agaaaaaaag atatttgagt cttccttcga taaaaatgat gtaaaaaaaa
      601 ttgattctgt cctatcgaaa tttccaacca aaatatatac aaatctcgga agttatgaat
      661 ctcagaatta taaaaataaa atgaaaaaaa tttttcacga ggtagggagt gaattttcta
      721 tcaaagagct aaactatgag cttgagtatg aaaaatctat ttgttcaatc ccttataata
      781 atattgagga aattatagaa aaggatgcta tcagcattta caaatttttt atttttattc
      841 ctaatcgtca attgaaagct aatatattta accatttgaa aaagatgcta aatgtgtctg
      901 ttactgaatc ggcacctgta aatattgaaa tcgtacctca aaatgtatct aaaggtgtgg
      961 tgtttgatcg tttggaatca atctacaatt taaagaaacc tttgagaatc gcgatcggag
     1021 atagtttaaa tgacttatct atgtttgaaa gtgcagatat tagttttgca atgggaaata
     1081 gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt ttcaaatgat aaagacggag
     1141 tatctcaagc atttgaaaga attcttacca tataaactaa ttaaggtagt atagaacata
     1201 gaattctaaa tacggaaaaa tggagcataa attccagtac aaatagtact tcttcagtgt
     1261 caaacgaagc gaatataaca tagatattta ggattgtgac aaaaatcatg tcacaatctt
     1321 tttttcgaat aaacttattt tttgaagtcg gccatttatc gtaatttctt tgcataatac
     1381 gaacaaattt tcggttattg atgaaaaaaa acattgaaaa tggtatgata aaagaaaaaa
     1441 aggaggaaat ttttaatggt gaaatttatc catgcggcag atttacatat ggatcggtca
     1501 tttgaaggac tgacaactct tgataaaact gtacaggaaa aactattgaa gacgaatctg
     1561 accgttttat caaatatcat tgaacaggca ataataaata acgttgattt tgttttactt
     1621 gcaggagata attttcatca aaatcgtcct tcattaaaaa tacagaaaca tttttcagaa
     1681 cagatgaaac ggttagaaaa gaaccatata cctgtattta taatattcgg aaatcatgat
     1741 ttctatcaaa aagagcggta ttggttctcc tttcctaaaa atgtccattt atttacatcc
     1801 gaaacggtag agacaaaaaa aataacgata aaaagtggag aaactgtctc tctcagtgga
     1861 tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga tggaatttcc ttctagagaa
     1921 ctcacagatt accatattgg gttgtaccac ggggaacctg gcgtctctca aaaaggaaac
     1981 tatgctcctt tccaaccctc aaagatgcaa gaaaagggat acgattattg ggctttaggg
     2041 catatccatg tgccaacggt cttaaatgaa aagcaaacaa ttgtttatcc aggtgcgcct
     2101 caaggtcata ctaaaaagga acaagcttct acatctattc tactagttga attaagtaaa
     2161 ggaagttgcc aaatacatcc gattaaagta gcagaggtct attggaagac aaaggaaata
     2221 tcattgagga aagcaagaac gacaaaagag gtcattgttc acattcgtca gcagctttca
     2281 aaagtagagg atggatttag tttaattgag ataaaattaa aagattatga ccatctcaat
     2341 acagatgttt tggaacggat acaatcagga gagctattag actatttgtt agaggaattc
     2401 tcagatagga taaatgattt ctttatttgg cgtatttctc tcatagagaa tactttttat
     2461 acgaagaccc cacttgctgc ttctgctaaa ttgatggaac agttatttca atactatcag
     2521 acacctcaag atttccaaca aattttaaat gaagtatata gtcatcaaga agcggctaga
     2581 atattgagcg aactgccgga atatcaagaa gaaacacttg aaaaagccaa gcagctgctt
     2641 aatcaagatt ttttatttga ggaggatcag gaatgaagat tttaaggatc gagattgctg
     2701 cttttggaaa atggcgtcaa aaaagttttg acttttattc aggcaatcag ctgatctatg
     2761 ggggaaacga agcaggaaaa tcaacgattt atcaatttat tcaagcaata ttatttggtt
     2821 tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa agacggctca gcctatggcg
     2881 gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc tgtcgagcgc tacaagcagc
     2941 aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt gggaagtgat gagttactag
     3001 aaaaactgat cgcacctttg acgaaagagt tattccagca ggtatttact ttccagcaag
     3061 agcagttgat ggaactagat catttgagag aaaaagagct acatgatgcg ctgatttctt
     3121 taggcatcac gggcagttct caagtattcc aaaagcggca agaatattat cagaaagcac
     3181 aacaattatt caagaaaaaa ggccaaaaat tactaatcaa tcaaaaatta aatgaatgga
     3241 aagagctgca agaaaaaatt catcaaaaac aagaacagga agcttatttt ggcaagttga
     3301 tccaacaaga acaggaatac gagtcgcaac agcagcagtt attggcacaa ttaaaagagg
     3361 cgaacaaaca attttcatta gccagacagc aagaacttaa tttttcttta tatgaagaat
     3421 ggcaatctct caaaaaaaac agcactacag gtcttcctga tgagcaactt cttttagatc
     3481 tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg tttggaagaa ttagaagcaa
     3541 cactggaaaa gcatagcggg atggatcagc agtcagccag atattatttt tatttagagc
     3601 aggagcagga actaaaagat ctccagcaac ttcaatttac agcggaacaa ttaaacgaag
     3661 aatggcaaag attacaaatt gagactagag aaacaaattc agaattgaag attttggaac
     3721 aaaggtggca ttggcagaaa aatccgttac cggaagaatt tttatatgaa gaagaatgga
     3781 agcatcttct ggctgaagaa agaaggattg ctgatctaga aagccagatt tctgtgcgtc
     3841 tccaaatagc taaagaacag caagagatga ttgaagcaga agtgaatgac ttggaagaga
     3901 cctatccagc attattacaa aatcagtcag agatagcacc agtctcttca tctaaaccgt
     3961 atatgctgat ggctggcgga gcaataatca ttgcaggtat atttgtacca atgcccttga
     4021 aagtgatcct tttactaatt ggtgcaggca tgattggcta tttcttctac aaaaggcaac
     4081 caaaaattaa aactaaaaat gatttctctg agataaaagg aatgtggcaa gaaaaactag
     4141 gacaacttga tctagcctta gctcaattag ctgaagcaga agaaaaacaa aagagaactg
     4201 gagaagaaaa acagattctt cagcaagaag tatatcgttt tgcggatcat cggcacttag
     4261 gaaagatgga tacgctcaaa ctcatgaaag aatatggaca ggaagtcata gattatcaga
     4321 aattatatgg cgtatttgag cggaaacata aaagagaaca ggaaatacaa gaaaaactac
     4381 gacaaatcga ccaaaaattt gagtttgtta aagaatggtt accgatacaa gataagacaa
     4441 tttcagaaaa aatggtattg ctttcccgtt ttatacaaga gatggaacag atgaaatttg
     4501 ccagaagcta tcagcaaaat actttattga agcaagaaat caatcaattg aaaaagaaac
     4561 aaaaagtatg gatacaagaa tacagcgaac aattgactca agcaggctta tcttatcctt
     4621 cggaagtacc tgtcttttta caaaaagcca aacaagccga acagcagatg aaaagataca
     4681 atgaattatc ggaaatgctt actccactat ttcctgaaga gctgacaaaa gagaaattat
     4741 ctgtaagact gcgccaactg aaagaaaagc aatcccaact ccagcaagaa gaaaaagaag
     4801 caagtgaaaa tcggcaacga ctccaacttc aagtagaaga gttgcaaaga gacggcacat
     4861 tggacgaact ttatcaacaa gctagccggc aaaaagcaaa attacaggaa ttactggagg
     4921 aatggggagc acttgaaatc gccgggacat tcttaggtga tctttcaaca gagatgagtg
     4981 aaagacaatt accccagttg ttaagagcag caggtgatta tttaggtatt ttaacaaaag
     5041 gaaattatca gaagatatta ctgaaaaatg atacactgac tttaacagac ggatatacag
     5101 aatttcccat ctatgagtta tctacaggta cgaaagatca gctgattatg gcgatgagat
     5161 ttgcttacct ctcattagaa gctaaccgtt cgatatgccc gatcatcatt gatgatggct
     5221 ggcttcatta tgatcatcag cgaaagtatc aattagctga acttttagct aaatttggag
     5281 aaaagtatca aataatttgt ttttcttctg ataaagaaat ggtaagctat taccatgaga
     5341 tgaaccaaat cgtaaaacaa ttagaaggag caaataatga aaaaaattcg tgatttagta
     5401 atcgatgagc agtttgaaca atttgtcctg atcaaaagtg cagatgtaag aatcgctaaa
     5461 aacggaaaaa aatttattgc ttttactttc caagatactt caggtactat cgatggtaaa
     5521 tactgggatg cttcggagga ggaaatcaat aaatttacag ccggcacagt cgtttttcta
     5581 aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa agattcttca tatgcgctta
     5641 acaagggtag atgagccagg agagcctagt ttatacatgg aaagagcccc tttgaagaaa
     5701 gaagaaatgg aagaagaaat caatcaattg ttgtttgaga tcaccaatgc gcattggaat
     5761 cgtattgttc gctatctact taataaatat caaaaacaat tttttgagtt tccggcagca
     5821 aaaagaaacc atcatgcatt tgccagtgga ttagcttatc atactttgac aatgcttcac
     5881 ttagggaaat caatcgccga tgaatatcct gagctgaatc gagcattgtt gtatgctggt
     5941 atcattttgc atgatttagg aaaggtgatt gaactttccg gtcctatgtc gacagaatat
     6001 acgcttgcag gaaatttgat cggtcatctg gtattgattg acgaagaaat aactaaagcc
     6061 tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc tcgtattacg tcatatggtt
     6121 ttagcacacc atggcttatt agaatatggc tcacctgtac gtccaagaat aatggaagca
     6181 gaaatattac atcaaatcga caatatcgat gcttcgatgc agatgatgtt gacttcgatt
     6241 cgtcaaacag aaccaggaaa atacaccgat cggattttcg gtatggacaa ccgaagtttc
     6301 tatgtaccaa aagatttata gcaaaaattt atttttttca ttcaaaacaa aaaaagcgag
     6361 acataagtct tcgctttttt tgttttcttt tattttgatg aatcagttgt ttcttcagtt
     6421 gaagattctt ttgttgaatc agttgatttt gtgtcagaac tctttgttga agctgtagtt
     6481 tcacttgagt ctttcgtact tgatgaagtt gttgtgaatg cactcaacac attttcaaag
     6541 gcatcatctt tgatttttac atttgcatct tttaattctt caccgattac tttagttgtg
     6601 aatgcattgt cacttagttt agtttcagta gcgatatctt ttagttgatc tttgtattta
     6661 tccatgtcat tgcctttatt ttggtttttc accattttga caacgtagta ttctgtcgca
     6721 taagaagtag gatttgttgt agtaataaca tcggagatct caccgtcttt caatttgaaa
     6781 gctgcttctt ttacttctgc aggaatagtt gttgtcgttg agtcgaattt aactttaccg
     6841 ccatcttctt ttgtctctgt atcagttgat ttgtctttgg ctaatttaga gaaatcatct
     6901 ccatcatccg ctgatttttt cacatctttt gcttcatctt cacttgataa tttgatgatt
     6961 tgtgcttcta cttctggatg gaaagatttc caagcagttt tcaaatcgtc atctgtaata
     7021 tctacgtgag ctttcaagcc agcttcaaaa gctaaattgt tacgaatata ttctttataa
     7081 gtatctttcg tatatccagc agcttctaat tgtgattcaa aagtatcacc taatgatttc
     7141 gcttgtttgt cgtattctgc atccacttgc ttatcggtta ctttatcgcc atatttgtta
     7201 ttgaatactt tataaatgat catccgttgt actaaggatt ggttcgatga ttcaagtttt
     7261 gcttcatcat agaaatcaga tacagtgatc gttccgcctt tcatcgttgc gatatcttta
     7321 ttagtatcgc ctgaacaagc tgctagagtt aaaacagcca acgcactagt agctgccaaa
     7381 ataattgatt tttttttcat taaaatttgc actcctgtaa ttagttatta tttttatata
     7441 ttaactatac catatcccat ttcctttaaa aacgttgtcg tggtagtttc aaacaaaatt
     7501 caaaatttca tcacattcct aggaaatacg caaacttttc tcacttatat agttaaatct
     7561 gttgagaaaa taatcattat tatatttgtt caggatactc tggcagatca gcttttagtt
     7621 tttctacttg ttcattgatt tgtgtgatac gtggttcggc ttggaattta aaatcttcga
     7681 tatctttttt gattgcttct tggaaagcag gaatcacaga ttcagctgtt tcttttgttt
     7741 cgatcaatgc gcttttaaaa tgattcaggc tgttatttaa atcatttgtc agagttgttg
     7801 cttcttccaa ttcttcgatc agtttctttc ttgtttcatt cccgctcctg ggcgccaata
     7861 acagacctcc gacagtgcca gcagcagctc caaataacag tcctttgaaa aagtttttaa
     7921 tcatcgcagg acacctgctt tacgattgtt tcttgaatag cttttagttc ttccgatcca
     7981 taactatctt gatgattgac aaagtgaata gaaaaatcat cctctttgct gtagcgaggg
     8041 actaaatgga tatgtgaatg aaaaacagat tgataggcta gttcacggtt gttattcaag
     8101 atattaagac ctttcatttc tggaaaagct ttttccaatg cttgagcaat cttaggcaca
     8161 cgcgaaaaaa cctctcctgc aagttcggga tcatattcaa aaatatctgc tacatgacgt
     8221 ttaggcacaa ctaatgtgtg tcctttcgtt gcttgagaga tgtctaaaaa agcatagacc
     8281 ttgtcgtctt catatatttt atagcttggg atttcttgat tgatgatttt gcaaaaaata
     8341 cagttttcca tataaaacat cctttctata aagaataaat acttcagctt aactttacca
     8401 taagttaaag ctattgtaag gaaaaaaaca aaataccgtc attttcttcg tggtataata
     8461 aataaaaata aatggcggag gaaaaagaat gagtttaatt atcgatcatg tcacaggggg
     8521 gtacggacat ctccctgtgt taaaagatat cagtttcgat gtacagcctg gagagatagt
     8581 agggttgatt ggactgaacg gagcagggaa aagtacgacc atcaagcaca ttatcggtct
     8641 gctggaagct tcaaaaggaa cgatagaaat caatcatcta tccttaaaga aaaatacgga
     8701 agaatatcgc aaacaaattg ggtatattcc agagactcct tctttatatg aagaattgac
     8761 attgagagaa catatcgaag tcactgcgat ggcctacgat atacctatgg atatagcaat
     8821 gaaaagagca gaaaagttgt tggagacttt tcggttatct aatcgtcttg actggttccc
     8881 agctaatttt tcaaagggaa tgaaacagaa agtgatgatc ttatgcgcct ttttgatcga
     8941 gccttcttta tatatcatcg acgaaccatt tttaggcttg gatccactag ctatcaatgc
     9001 attgcttgaa ttgatggtag aaatgaaaaa agaaggagcc ggtattttga tgtctacgca
     9061 tattttagct actgcagaaa aatattgtga tcgttttgtg gtcttacata acggagagat
     9121 acgagcagac ggttctcttg agatgttgag aaaagaattt cagctgccgg attcttcttt
     9181 ggacgaaatc tatattgctt taacaaagga gcaggcacat gagtagtttt tttcaaaaac
     9241 gtttagcaag acatcaaaag aaaatgatga aatatatgcg ttatgtattg aacgatcatt
     9301 ttgttttagt ttgtcttttc ttgattggcg gtgtgggatt atattattct aattggttga
     9361 aaaccctgca gccaccgttt gcaattggag ggctcataat ctctgtattt tggatgctct
     9421 gtttattcgt gggtaagatg gctacttttg cagaggcagc agatattgtt tttttacttc
     9481 ctaaagaaaa agaaatgcaa acctatttgg aacgagcttt tcgctattct tgcatttttc
     9541 cgttgatctt tttgatattg gcatgcggat tggccatgcc attagtggtg gttagtacgg
     9601 agcaatcctt ttcgatgttt ttcgtctatc tgatcatatt atggagtttg aaattcagtc
     9661 atctgcaaat caagagagca gaattgtttc gactagatca acaagttatt cgttattgga
     9721 aattaggctg gtttttttct tctctgatct ttttgtttct atcattttat tggctaatat
     9781 gggcaggaat agcaggtgca ttagtacagg caagtctata ttactggttt ttgtggaagc
     9841 aacaagaaaa aggattggat tgggagaaaa tggtgcatgt agaagaacat aggttgcatc
     9901 agatctatca atttatcaat ttattcacag atgttcctga aattacggca aagatcaaaa
     9961 gaagacgcta tttggatcct atattgactc atattaaaag agaatcaaaa aacacttatc
    10021 tttacttgtt tgcaagaaga ttggcaagag gttctgattt tagtggtctt tatttccgat
    10081 tagtaattat aggtgggtta ttgattgccg gagtagtcga cttttatttt agtctgatta
    10141 tcggtttctt atttttatat ttgatcggtt tccaactgtt acctttagcg aaccagttcc
    10201 gctatatggt tctcgttcag ctttatccaa tccataagaa tcagaagaag caggcgattc
    10261 agtatctact cacatgggta ttaattatcg cgtcggttat ttttggtttg attgctgcag
    10321 tcgtattaga tgggcagcag cgatgggttc cgctagcggc ttatttgatt gttactatgc
    10381 tctttgttaa actatatgta ccaactcgtt tgaagaagat gtcagactga aaacaaagct
    10441 gtgtagggta aaaatgagaa tgaaaaatag agcgttgaca gtaagaatgc tgttcgttct
    10501 attttttttg agaaaagctg gttttttgaa tggcaattaa atgaaaaaga acgtatatgt
    10561 gcttgacgtg tagtgttcag gcgactagaa taataagtac tgcaaaacaa agagtaagag
    10621 gacgggagaa tgcatgatgg agtttcagtt agaccaagag tggcggttac gtcccatcaa
    10681 aggcgacaca ggaaaaacat acataggcat gcgtgatgag gacaaagtct ttattaaacg
    10741 aaacacgact ccgatgttgg ctgctctttc aagggaagga attacaccaa aacttgtttg
    10801 gacaaaacga acggggaatg gcgatacttt gacagcacaa gagtggctgg aaggtcgctt
    10861 gctgactcct aatgagattg gtcaaagaaa cgacgtgatt gatgttttgt accatttaca
    10921 tcattcaaat tctcttaaaa ccatgctgac ccgtatcggt ggtcgtgtca tgacgccgga
    10981 gaagatgctg caaaaatatg aaaaagaatt accggaagaa ctccagaaaa atcaatttct
    11041 gcaaattgtc catcgttttc ttcgtgagca gatcccgtca atagataagg aaaacttatc
    11101 agtagtacac ggagatgtaa accatcgtaa ttggttagta tgtaaaaatt atttatatct
    11161 agtcgattgg gattcaatca tgtttgcaga ccctgcggtg gatattgcaa cgattttagg
    11221 aaattatgtg ccactgtcta gctggaatca gtggctgatc agttatggca tacgcccgac
    11281 tgacgaaatg cttgaaaaat ttcattggta tgcggccatg aatttgctcc aagaaatcac
    11341 cagatattgc aaacgtgaag atcatcgacg gatgaacgaa gaaattttac aattaaaacg
    11401 aattttcagt ggctgaggtt tggcccaaac tctgcggaga tttggccaga cctttttaat
    11461 tagaggaaag gaatggagat aaataatgcg tgtccgaaat cgtccgggag cagcggaaat
    11521 gttagcggct cacccgaatt ttgtaattag tgatccaaca ttgtggaagg gaaaatggaa
    11581 tgaattgttt gaaaacgatc atccaatcca cattgagatt ggtatgggga aaggccagtt
    11641 catcactgga atggcaaaag cccatcctga aatcaattat attggagtag aaatgcaggt
    11701 cagtgttgtt tcgattgcac tagacaagtt gatcgaacaa ccgcttccta acctgaaact
    11761 tctacatgtg gatggatcag cgttgacaga gtatttcgct gacagcgaag tagatcaaat
    11821 ctatctgaat ttttccgatc catggccaaa aaaacgtcat gaaaaacggc gattgacata
    11881 taagactttt cttgctgtag atgaacagat tcttcgtcca aatggagaaa tccatttcaa
    11941 aacagataat caaggattgt ttgagtattc tctggcaagt ttttctcaat atggcatgat
    12001 cctcaaacag gtatggttag atttacatca aagtcaattt gaaggcaata ttatgacgga
    12061 gtatgaagaa aaattttctt caaaaggtca acgtatttat cgagtagaag ctcaatttca
    12121 agataagacc aaaaagaaaa gtctgtgaaa ctaactttct gctttcataa aaacattaat
    12181 atccataaat aaatcccaga aaatgaacat tacagatgta atgtattttc tgggatttat
    12241 ttgatttaaa atgtatttat ttcatcggtt tttccacaag gtggaatcaa ttggaagcga
    12301 gaaaatgaaa ataatccttt gtcggtgatc ttcaattcgg gtatgacaga taatgttaag
    12361 aaagaaagtg tcaaaaaagg atcaaaggtt ttagctgctc ctagttgatg tgctttagtt
    12421 attaattcat tcaactgttc atagacgatc gaaaaaggtt cctgtgtcat gattccgcct
    12481 ataggaagag aaacacaagc tatttcctgc tgtttgttga caacggtcat cccgccacct
    12541 ttttgaatca aaagattagc agcatacagc atatcctcgt cgtttgtccc aacgacaact
    12601 agattatgcg agtcatgggc aatcgttgtc gctatcgctc cttgtttcag ctgaaatcct
    12661 ttcactatgc ctaatccaat gtttcccgtt tgatggtgac gctcgatcac cgcaatcttt
    12721 aattggtctt tttcgattga aggaatgaat cgttcgcctt gtcggtccac tttttctacc
    12781 aaatctttag tcactagact attcgggata atttcgatca catgggcttg tgaagaagaa
    12841 agaagcagct ctaacgaatg tggatttaat ggttgagcgt ttagttttgg cagacaacac
    12901 gtatatgaat ttgaatgatc agcaaataaa tggtcttgga gttgcccaga ttgaacaact
    12961 aatgttccct tatagaaaac ttcatgaatt ggcagcttgg taagatcgtc tgttaagaag
    13021 aaatcagctt gatacccagg ggcaatcgca cctagtgttt tcaaatcaaa acattctgct
    13081 gcattcaatg ttgccatctg aatggcttgt aatggatcga ttccaagtgc aatagcttga
    13141 cggacgatat gatcgattga tccttctttt actaaatcat tgatcagttt atcatcggtg
    13201 acaaagaagc atcttcttga attttcaggt gtgatcgccg gtagtagcga tgccagatct
    13261 tttgcaacgg tcccttctcg aaccattaaa tacataccaa gatccaaacg ttctttagct
    13321 tcttttgcag ttgtagcttc atgatccgtc cgaatacctg ctgcaggata aacatttaac
    13381 tcttccatgc cgataccagc tgcatgcccg tcaatttttt tcttatgctg gtgcatcaat
    13441 tggatctttt ctaggatgtc tgtttcatta tttgctactg cttgatagtt catgacttca
    13501 gctaagccta aaacttcggg tttttgtaaa aaaggggcta atttttttgc atttaaattt
    13561 gcaccattgg tctcaaaagg ggttacaggt acacaagaag gtagcataaa aaagatattc
    13621 atgagtgttt gtcttgcatc ttctatcata aactcaagac ctgtactccc agcaatgttt
    13681 cctatttcat ggggatctgt cactacagta gtaactccat gaagcaagga tacttttgaa
    13741 aattcgcttg gtgataaaag tgagctttcg atatgcacat ggccatcgat gaatcctggc
    13801 actagatatt gcccattagc attatgtacg ctttttcctt cgtattcacc gattcctacg
    13861 atcattcctt cgcagattgc tacatctttt tccatgattt ctttcgtaaa gacgttgacg
    13921 atcttagcat gcttgatcac taggtcagca ggcttttttt tacttgctac ttcgatatat
    13981 ttccttaatg ttgtttccaa tggagtgcct cccaagataa atttttataa gtataacata
    14041 aattcggttt ttgccaatat ttttttgtaa attcatttta tttgaaacaa aaagatcaaa
    14101 aagacgtatg ttttttgtgg aggggagaat aatggaaata aaacgattat cggatcaaat
    14161 gataagtgaa agttttgcca aacatgccca agacgtatat atattgccag cttcagaagt
    14221 attttacatc tattttcgct atggaaaaaa acgaacattc tggaagaaag tctcttcggc
    14281 agatggacag caactgatct caagatttaa gtatttggga cagatggata tcggagaaaa
    14341 aagaaaagct cagttaggtg catttactta ttctttagaa aaacaggaag ttcgtctgcg
    14401 gttgtcttct gtaggagatt accttcaacg agagagttta gtgattcggc tattatacgg
    14461 tacttttgaa acaccccatc gttgctttag ttctcgagat tttgcaacta ttttacagaa
    14521 aaccgtcaga cgtggattat accttttcag cggaccagtc ggatcaggca agacttctct
    14581 aatgtatcgg ttggctttgg aagaaaaatt gcaagtgatt actatcgaag atcctgtaga
    14641 gatagaagaa ccgttttttc tgcagctaca gatcaatccg aaaatagcac agacttatga
    14701 tacacttttg agactcgcat tacggcatcg tccagacttg ctaatcattg gcgagataag
    14761 agatgaatca actgcacatg ctgcggtaag agcagcacta acagggcacc gggtttttgc
    14821 aaccgtacat gcacgcaatc ttgaaggtac attggtcaga atccatgaac tgacaaagaa
    14881 aaaagaagag ctatctgaat gtttagctgg tgtcatttat caagaacttt tggaagatgc
    14941 caatcaccat ccttctgttt tatggagtta tcaatttatg gaggaagaat cagtaaaaaa
    15001 atcatgggag gaaagttatg aagaagctca ggaaaatatt tggcagtcag aactattcta
    15061 aaggattaac aagaagccag caaaaccagt ttgcacaaac catgggagaa ctgcttttat
    15121 ctggttttag tctgcaagaa tccctttatt tattactcac gatccgttcg tttccacctg
    15181 ttattttggc aaatatccaa catcagctaa aggaagggca agcgtttagt gaggtacttt
    15241 cgcaaatagg atatggagca gagcaactgg tacagatcga gttagctgaa cgtcatggga
    15301 atctgccgac aactttgttg gaaattgcca aacagtttcg gcttgttgaa ggatttcgca
    15361 atgatttgaa aaagctgctt gcatatccga tgctattgct cacctttttg ctaggtatct
    15421 tgtttagtat gcggttgttc gttttgcctc agcttattgg ttcaggaatg gtcatgcaag
    15481 agcattgggg tattcagctg atacaagtct ttcattggta tgttctacta ttcatcctag
    15541 cggttactct attggcttta ctagtaaggt atcgtctgat gaagttgtct cctgttgatc
    15601 agagtgaatg gataagcaga caattattga ttggaccgat atattccttg tatcaatcgg
    15661 cttatttgtc actagagatt ggaaagcttt ttcatgaagg tttcgaatta aggcaaatca
    15721 tagtctgctt aaaggaaacg aaacaagaaa gtctgattca gcatcttgcc tctaagttga
    15781 ttcaaggatt ggaggcaggt atgtcattgg ctgagcagtt ccagaattat cgttttctat
    15841 cagaagaatt cagtaaaatt gttttacagg gagaggcaaa gggaagttta ggtaaagaac
    15901 tgatttttta cagcgaatgg acaagaaaac agcttttcaa acgtcttcat cactggatgc
    15961 attttattca gccaatcatt tttttgggcg tagcggcttt gattttatta gtttatgccg
    16021 ctgttttatt acctgtttac ggaagcatag aggaggtttt gccatgaaaa agagaaaaat
    16081 atcttattat agcggtttta cattgatcga aatgctaatc gtcttactga tcatcagtgt
    16141 attagtgttg ttgttcgtcc ctaatttgtc gcgttatagg aatcatgtag accaagaaag
    16201 tcgtgaagcg atcatccagt tggtagacac acaaaaagag ctctatgcgc ttcaaaataa
    16261 tggaagagtt cctactgtgg aggaattgtt gaatgaaggc tatatcaaac gagaacatgc
    16321 agaaatctat cagcggcctt gaaggtaagg ttttagcatc aggatatacg ttgtttgaat
    16381 cactcttact gattttatta cttacaacga tcctttcttt tccagttctc gcattttctg
    16441 catggaagaa tgaattggca gtctatcaat ttttcggcca atttgaaaaa cgaatctatt
    16501 ctacgcaaaa aatcgctatt gtgaatcaga tccaaacagg cttttattgg aatagtgaag
    16561 agaatcaaat catttttcat gttccgaatc cagataaaat aaattgggaa gtattagata
    16621 ttcctgatga gattaccttg aaacgccatg cttctattac ttttgctgct agaaccggga
    16681 atgaaagttc actaaaagct tatcaatttt attgggaaga aaaagagcgg acgattacgt
    16741 atcaattcca gatagggagt ggtcgctata caaaaagaat tgaataaaac aggatttgtt
    16801 ttgttggagt cccttacagc gcttatgatc gcttctgtct gtgtctactt agtgagcttt
    16861 ggccagctgt ttttgcttca ggcagaaaag aaaagtgggg aggaactgct atttattcga
    16921 atgatttatg aagatatcaa gacgcagcgg atgtatcaca aaagaccaga gcgaagggaa
    16981 gaaatgatcc catacgatat ccaaattgtc gataataatg gagtaatcga aaaagtgttt
    17041 gtcaatatgg agaaagaagc gatcagtatt gaaaaaacaa gtaattccag catttacgct
    17101 gattgaatgt ctaattggac tgatgatatt gagcagtcta ttgctcacat tcaatctttt
    17161 gattcagcaa accatccaag taagtgaatt tttgcagcgt aaagatcaaa aagagtggct
    17221 aatattttta gcacaattag aagaagaact caagagcagt cacaatgtct ctgtgaaaaa
    17281 tcagcaactg cactacacga tcgaggataa acagtatatc atcgaaagat atcaggcaat
    17341 gatacgtaaa agaagaactt ccggcggaca tcaacctatg ttaacaagta tcactgagct
    17401 tcaattattg gaaaaagaac acacaatttc tttttatgta cactttgaaa atggagaaga
    17461 tggatatgcc atatggacaa aaaacgatca gtagaaaatg ttataagggc tctattttat
    17521 tttctgtctt gcttttattt cttttatgta cgtttttatt gatggcagca ttagaaagct
    17581 accgtttgtc aatggatctt aatatacgaa tcaaaaatta ttatatagca aaaataatga
    17641 agcagatggc tttatcggaa cagtctacgc ttgccgaaag ttcggaagga gttttttact
    17701 acacaactgg atctgtaaca tatcagtggg tacaacagtc cctattctta gaggtggaag
    17761 tctcaccgtt tatctttcgg ttcacagaag aagtaaaaaa tgatacagat actagtacag
    17821 aatgatggaa tgttcgtgaa aacgccctct tttctgttat aacactgttt ttaataaaaa
    17881 aatgtttgcc gtttcacaaa taaaaaacag tgaaaacgat tgaaattttg tcaattgttc
    17941 gatagaatta aacaggtgaa aaaattgtta taatatatct agaaatgagg tgcaccatgt
    18001 taccagaaaa aatggaacaa gggtttaatc aaaacctgga agctattcag cttttacaga
    18061 atgcattggg aacttccttt ttagaagcct atgtcgaaaa tgcagaaaat ttaatagatg
    18121 attatcaagt acgtgtggtg gatggagtgc cgacaaaaga gacaacgcaa cggatcacag
    18181 ctctttatga agagttgaag aaactttctt tcgagccaga agaatggcgc agactgtctc
    18241 aattactttt attgaagggc agccagacag aacatttgca ggcgaaccat caactcactc
    18301 ctgatagtat cggtttttta tttgtttttc ttattgaaca actatacaca aataaaaaag
    18361 caccggttaa gattctggat attgcggcag gaatgggcaa cttgttatta acagttcttt
    18421 taaacctatc gaatgcgggg taccaaacag aaggaattgg tgtggatatt gatgatacat
    18481 tgttggctgt cgcagcttct acaagtgatt tgacacaagc aaatgttcaa tactttcatc
    18541 aagacgggtt gcaggaatta ttaatagatc ctgtcgattt tgcaatcagc gatttgccaa
    18601 tcggttatta tccgaatgac gaaaaagcaa aagagttttt aacaagtacc gaagaaggcc
    18661 atagttatgc acatcattta ttgctagagc aatcaatgaa atatgtgaag ccggatggtt
    18721 ttggtttgtt tttgatgccg agtggatttt tagaaacgga tcaaagtgaa gaaatcaaaa
    18781 aatggtttaa agaagaaggt tatcttcaag gaatgattca attgccagat gaattgttcc
    18841 gtaataaaca atcgcaaaaa agtattttga tcttacagaa aaaaggacca caagcacaac
    18901 aagtaaaaga agtcttgctt gtgaaacttg cttcattaaa agaaccagag aaggtgacgg
    18961 aatttttcaa cgagtttaaa aactggaaat catctgctct gtaatcattc aacgagaagg
    19021 agagtcatat gtctaaaaca attgcaatca atgctgggag ttcaagtttg aaatggcaat
    19081 tatatcaaat gccagaagaa accgtagttg cgaaagggat cgtcgaacgt atcggtttac
    19141 aagattctat ttttaccatc aagtacggcg ataatcaaaa attcgaacaa atcatggata
    19201 tcgaaaacca cgaagtagca gtaaaaatgc tgttagatca gttgattgaa ctgcaaattt
    19261 taagctctta tgatgaaatc accggagtag ggcaccgtgt tgttcacgga ggagaaactt
    19321 atggggattc agtagtgatc gatgagaaag taatggaccg cattgctgaa ttagcagaat
    19381 ttgctccttt gcataatcct gcaaacttaa tggggatcaa agcgtttaaa aagattttac
    19441 ctgacatttt gagtgtggct gtattcgata catctttcca ctcaacaatg ccaaagcaaa
    19501 attatttata tagcttaccg gtgtcttatt atgaagattt caaagtaaga aaatatggct
    19561 tccatggcac aagtcatcgt tatgtgtcgg aacgtgcggc aaaattattg aacaaaccga
    19621 tcgaagaact aaaaatcatc acttgccact taggaaatgg tgtatcgatc acagcagtcg
    19681 atggaggtaa atccatggat acttctatgg gattcacacc attagctggg gtgacaatgg
    19741 gtacacgttc aggtgatatc gatccagctg ttctcccata tttaatggaa aaattagaga
    19801 ttgatattga tgaaatgatc aatgttttga acaagaaatc tggtctgcta ggcctaacag
    19861 gattgtctag tgatatgcgt gatcttgaaa agaactatga aaaagaacat attcgtttag
    19921 cttatgacat tttcgtggac cgcatccgaa aatacatcgg cggttatgtg acaacgatga
    19981 atggtgttga tgcaattgtc tttactgctg gtatcggcga gaatgacgga catgtacgta
    20041 gtgaaatcat caaaggtatg tcttggtttg gttgtgaaat cgatccagcg ttgaatgaat
    20101 tgcgtggtga agaagtagat atctctacaa aagaatcaaa agtaagagta ttagtgatcc
    20161 caacagatga ggaactaatg attgcccgtg atgtggagcg tctgcgcggc taagccaaag
    20221 cattcgttag gataaaagat gaaacgcatg caagagggaa ttcttgcatg tgtttttttt
    20281 gcggagaaga ccgcaaaatg gtaatttaac ttcaatttcc cttctttttt acaggatctt
    20341 cttagggaat tttgcttgga aaagtggtca aaagggaaaa gactggcatt ttatttattg
    20401 attttcagga aaatcccaat attttatttt cattcatctt tataagggac attacatatg
    20461 taaaaagaaa aaacattttt agatcagcct cgtgggctgt cgactttatt cttcgcttta
    20521 tgtttagcta gaaaaccgat ccgaaacatt cattgatcga ataataaaaa cttgattttc
    20581 cacctgtact ttgcctgaat cgtgaaagca caggtatgaa aatcaagttt tttgttgcga
    20641 aaaaacgtcc tagtcaatca gttggccgag cggctggcgg ctgatctgct cctgatcaat
    20701 aacgagtgct tcttggaagt gttgctgcaa aaagtagcgg tcatggctga ctgctaggag
    20761 cgttccttga tagtcttgca gcatttcttc aatttcttct cgagcatagc tgtcgaggtg
    20821 attggttggt tcatccaaaa tcaggacgtt gattttttct aaaaagagtt tcagcaacgc
    20881 caaacggatt ttttcaccgc cggataaatc tttgatccgt ttacccacat cgctttgata
    20941 aaagccactt ttggctaatt gccgtcgagc gacttcttcg tttccagtca atgtgatggc
    21001 gtaagccagc aaacggctat cgggatcggc aaactgcagc tgctgcggca aataaccgat
    21061 ttgcaagcta ctacctaatt tgatggtgcc accgtaaggc ttttcctcac cgagtaataa
    21121 tttgagtaag gttgattttc ctgcaccatt gtcgccgatg atggctaaat gatctccacg
    21181 atagactgca aaactgctgt ctttgaatag tacttgatca gcgtactgct ggtgcaagtt
    21241 ttgaatgatg aaaacttctt tgccagattt tccaccatta gacaagcttt gcagccgatt
    21301 tttcggggga ggtggcggtt tgacgagtgt cagtttcgcc aggcggcgtt ccagttcttt
    21361 ggcttttttg aagaaggact cattgtctcc ttcatgtgcc cactgacgaa aacgacgaat
    21421 catgactttc aaccgctgga cttctttttg ctgcagttcg taattttttg tcagctcagc
    21481 caagcgagct tctttcaaga ctcgaaaacg gctgtagttt ccgggatact caatcaatgc
    21541 accttcttcc agttcccatg tttttgtggt gacttgatcg agaaattgcc gatcatgaga
    21601 aacgaccaga accgattttt ttgtattctg taaatagttt tccaaccagc gaatactgtt
    21661 aagatctaaa tgattggtcg gttcatcgag taaaagtaaa tcggcatcgg ctgctaaaat
    21721 tttggctaaa gcgactttaa ctcgttcgcc tccgctaagt tctgttaacc gtgcttcctg
    21781 acggtgtgga atccctaagc cttgcaagat cgctgcaatc cggtcttcta atacgtaacc
    21841 gcctagttct tcaaagcgat gctgcagctc accgtaacgg gccagacact tctctaagtt
    21901 tgtatctggt tctgtcatca atgcttcgta gcgactcatt tgctgtttga ggtcctgtat
    21961 ctcagcggca gaatggagca agtagtggtt gacggtcgct tcggaaactg taagttcttg
    22021 ttcgaccgtc ccgatcttta agcctttttt gcgactgata acaccgctgt cgacaccttc
    22081 tctgccggtc aagatttgta aaaaggttga tttgccagtt ccgttgatac cgactaaggc
    22141 aattttttca ccagcttcaa tttgacagga taaatcctca aataatggat tcccgccgat
    22201 tgttttttta atagaaattg cttgtatgat catgatctct ctccttgaga ccactcaaaa
    22261 aggccatggt tatccatggc ctgtgttatt taaactcaga ttatcatgag aaaaatacct
    22321 tgagatggcc attgatttta cgtgaattca actgttttgg acaaacttgt cccgtcaaca
    22381 gcaactgtaa aaccaattgc atggctaaat gcgatatggt atcgttggta attttcatta
    22441 cttgttattt tagtcatgcg catcatagta ttttccctcc ttcgtgtagt tgtcttttcc
    22501 agtgtacctg aaagtgtcag aaagttcaac tagcgctgac atttacttaa tatttatcga
    22561 tgtgtaattc tgtcagagag ctttttttat ctgactgagg gtaatgtctc gtggaccaca
    22621 tcattgtatt ctgagctaaa ccagcgttca ggcgaaatca tatggtgttt gataaaaaac
    22681 tttgtctaca tgacttcctt ttgccatttg cttaataccg ttgcattaaa cagctcttct
    22741 ctttggaaat ctcaccaaca tcgcgttcag gagtatcgtt gtggttgcaa tggcaagcgg
    22801 tagcctttca tcaattcttg aatgatttac cgaatgtagc acttgccttc tcgcggtacc
    22861 cgaacccgaa ataaaatttt agtttggcga taaaatcggt atccgccatc gatacttcat
    22921 attttttcgt atcagattca tcggaaactt ccacattgac gaaccaataa gctttggcat
    22981 gcttaggtcg ttttaaacca caccagggcg actaatcgcc ctggtgttca ctttttttta
    23041 ttttacttga actcattgac tgttttccct ttattttaaa cattataaag accgatgagt
    23101 gcttgccgcg tcttttgttt ttatcacggt tttttgtgca aaaaaacctg ccgatagttg
    23161 gcaggtcgtc tacgtgttaa gctttggtgt ggtctttagc tggcaattgg ttttctaaat
    23221 cagttcggac aacaagcaca tcgcaagctg cgtttctaat aacatattca gaaacagaac
    23281 caataaataa acgttctaca gcattcaatc ctgtggctcc taacataatc aaatctactt
    23341 gattatcttc tgggatttct ctggcaataa tttgttttgg tgagccatat tctacgacag
    23401 aggttacatt attcaagcca gctttcttcg cattgctttc ataatctgcc aatgtttgct
    23461 ttgccatttc agtagcttgt tctgcaagca ttccatcaaa ggatgaaacg gtttggaaag
    23521 aacgtgtgtc aatgacatgt gctaatagaa gttctccgtt atttcggata gctacattga
    23581 ccgccttttt aaaagctaat tcagcttcat cagaaccatc tacggcaacc atgatttttt
    23641 tgtattgttg taacataagc attcactcct caagtcaatc gtattgtact cttttattgt
    23701 agaacacttt agggaaaaag agaattattt actgctttgg ttcagcaatt ttttttgcaa
    23761 atcggatact gaaggtggca gaaacaataa ttacgatcag taaaaagacc aaggcaataa
    23821 agcgttgatt gaaaaaagct gttaaaatac ccattacgcc taaaacagca tatgcagctc
    23881 cgtaaaattg taggaatcct tgatttttat catttttgtt gatcaataca aaaaagatat
    23941 cctgtttatt aaataaataa tagctaacga ataataatac taaagtggcg ataatcatta
    24001 aaatcctaat catgaacaaa tcctccaaaa taagcaacaa atagaaaaag caatttgcca
    24061 agagcaaatt gctttattct ttaaaagatg gtaatctccg ttaatgccgt tgtttgtcct
    24121 gtagtattga tcccgcgaat tcaagcaggt gggtcccatg gtcgtagctt cgaactacca
    24181 aatgataagg catgttacca gcagtgacac aagacgggtc ccaagatatc aataaaaatg
    24241 ttcggtcaac acacaaagag gacaattcga acatcacaga tttcccacct ctatagtagc
    24301 atatgaattt aaaaagagca atgagagagc tcgcagaaac agaaccgttt tcttgtctag
    24361 ttttttggag gatgcttttt ccactctttt atacggtaat actgttgacc cagagcctgt
    24421 tcatatttcc ctgtatcttt tggctgataa tactgagcat tcttcagttt atctggtaaa
    24481 tattgctgat tgacccaagc ctgatcaaaa tgatgaggat actgataacc cacgccacgg
    24541 ttgagtgatt tcgcaccttt gtaatggcta tctctcaaat gatcaggaac atctccagct
    24601 ttgccctctc gaatatcagc aagtgctgca tctaatgcca tgtaggcaga attggatttt
    24661 ggcgacaagc aaagatcgac gacgacgtct gctagtggga tacgtgcctc aggcaatccc
    24721 aatttttcag cagctaatac agcgttcact gtacgagccg cagcagcagg attacctaaa
    24781 cctatatctt cataaccgat aaccatcaga cgccggcaaa tacttgccag atcacctgcc
    24841 tcaactaaac gggcgagata atgaagtgca gcatccacat cacttccacg aatcgatttt
    24901 tgaaaagccg aaataacgtc ataatgagca tctccatttt tgtcatgggt aagagctttt
    24961 ctttggacac attcttcaat gatcgacagt gtaagatgaa tccttccttc tttatctgaa
    25021 ggagtcgata gtgtagccag ttccagccca ttcaatgcac tccgcaaatc gccgttcgtt
    25081 gcacgcgaaa ggtgaagaag agcatcttcg tctaactgga ttgcttgttg tccaaggccg
    25141 cgttctttat cccttaaagc atgttcgact gccaactgga tgtcttgttc tgttaatggt
    25201 ttgacttcaa agatctgtgt acggctgcgg atcgctggat tgatggtaat ataaggattc
    25261 tcagtagtcg ctccgatcaa gatgattcgg ccactttcta aatgaggaag cagaaaatcc
    25321 tgttttgttt tatctaatcg gtgtacttca tccaataaga ggatcactgt accactcatt
    25381 tttgcttctt ccgctacaac ttgtagatcc ttttttgaat ctgtagcggc gtttagcatg
    25441 cgaaatgcat aattagtaga gccggcgatc gcacttgcga tactcgtttt tccagtacca
    25501 ggaggtccat atagaatcat ggaagagagc atgcgagcat cgaccatgcg gcgtatgatt
    25561 tttccttccc cgactagatg ctgctgacca acgacttcat cgatcgttcg aggtctcatg
    25621 cgataagcca atggctgttg cataggattt tcctccttct tgtttttacg tcccttaatt
    25681 atatcatatt tagcaagatg cctagaaaga acagccttct gttgttttgt tttcatagct
    25741 tatccaacta atattgtgta tgataaagta ggtgagaaaa atgacggata aacaaaagat
    25801 agatgaattt ttttggaata aaacgacgcc agaagtggca cgggatttat tggggatgta
    25861 tttggagcat gacacaccag aaggaagatt agctggatat attgtagacg cggaagcata
    25921 cctaggtcca gaagacgagg ccgcccatag ctatggttta agaagaacac cgcgtgtgcg
    25981 ggcaatgtat gaaaaaccag gtacgatcta cctttacacg atgcatactc atcggatttt
    26041 gaacatcatt actcagccag aagggattcc tcaaggagta atgatacgtg caatcgaacc
    26101 cgccgcaatg attgatcaaa tgagtaaaaa tcgtggcgga aaaactggtc cagatatcag
    26161 caacggtccg ggaaaattag tggaagcatt agcaattcct caagaacttt atggtcagtc
    26221 tatagcagat agttcgttac gcttagtttt tgagaaaaaa aagacaccaa aaaagatcaa
    26281 tgctttgcct agaatcggta tcccaaacaa aggtgtatgg acagaaaagc cgttgcgttt
    26341 tgttgtgtcg ggaaatcctt atatctcttt acaaagaaaa aaccaaatcg agaaagattg
    26401 gggttggagg aaagaaaatg aaaaagaagg atctattaac atatttagat gaaataattg
    26461 aaaaacaagt gacagactat gatgtagctt tagattggga tacaaaaaat catacgatcg
    26521 agatcgttgt tcgcttgttt gcagaaaata aagcacaatt tgagattgat gatgcagaag
    26581 ggatcgtttc gcaagaacca attatcgaat ttgaagacgg cgtacttctt tttaatcctc
    26641 agaaatctgt ttttgatgaa cgcaattatc tagcagtaat cccttatgaa ggaaaaaaag
    26701 ggttggcaaa atcagtagct gattctttag tggaatatct aaatgaagta ttagctcaag
    26761 ggcaaagcga tttgcttgat tttttggatg aagacgatga cgaagctgta ttcgagcttc
    26821 actgggataa tcagcaacta gcagaattag tagaagtaaa acagcaaaat gaagcagata
    26881 cttatttacc gtacccaagt tattaaggag tgacagatat gaaatggaat gaagtaaaag
    26941 tagaaactga aagtgaagca gtcgaagcag ttgctaatat tttgatggag gcaggggcga
    27001 gtggtgtcgc gattgaagat tctttagatg ttgaacattt tcagtcggat caatatggtg
    27061 agattttaga taaaaatgat tttacatcgc tgaaagaagg cgcactagtc atggcttatt
    27121 ttccggaaac gactttctta ccggaaatct tgccttttat caaagatcgt atcacacaat
    27181 tacctgagtt tggtctagct attggaaaaa ataaagtaac agttagtgaa gtagaggaaa
    27241 gcaactgggc aacagcctgg aagaaatact atcatccggt gcgtattacg cgaatgctga
    27301 cggttgcgcc aagctgggaa aactaccaga cagaagatcc tctagaaaaa attatcacac
    27361 tagatcctgg tatggcattt ggcacaggca ctcatcccac gacttccttg acattgacag
    27421 cactagaaac agtcttgcgt ggtggtgaaa cattgctaga tgtgggaact gggtcgggcg
    27481 tgctaagtat tgcagcaaaa tatcttggtg caaaagatgt ctatgccttt gatttagacg
    27541 aagtagccgt tcgttcggcg aaagaaaata tggatatgaa tgaaatagcc aaagatgtcc
    27601 atgtatcagc taacgatctt ttgacaggaa tcgagattga aagtgatgta atcgttgcga
    27661 acattttagc agatatcatc ttattgatga tcccagatgc ttggcgttta ctgaagcaaa
    27721 caggcacgct gatcgtttca ggaatcattg aagagaagaa gcaaatggtt ctggatgcca
    27781 tgttagaaca agggtttatc gtggatcaaa ttttccagca aaaagactgg tatgcaatta
    27841 ttttgaaaaa accagaggtg gaatagatgc agcggtattt tttaaaagaa tcatatgaat
    27901 caaaagacta ttttgtttta gaaggggaaa actatcacca cattgtacgg gttatgagga
    27961 tggaacccga ggatgaagtt tttttggcat tcaatgatca aatagccatt gttgcagaaa
    28021 tcacggagat tactgaagag gctgtttatc tgaaagaaat taaaaaagaa gaaaatcaaa
    28081 aggaactgcc aagttcgatt accatagcga gtggctatcc taaaggggaa aagctggatt
    28141 ggatcgttca gaaatccact gaattaggag cgcaagcttt tcttggtttc ccggcatcag
    28201 cctctgtcgt taaatgggat gcaaaaaaac gtatgaaacg acaacaacgg cttgaaaaga
    28261 ttgctcaaga ggcagcggaa caatcccatc ggcagatcgt gccatcagta gagttattgg
    28321 aaacgaaaca agatttattt gagcggctag atgattatga cgtggtcttg gttgcttatg
    28381 aggaatcagc aaagcaaggc gaaaaatcaa atgcagccaa aattcttagt gaaacaaaac
    28441 cagatagtcg aatcttagct gtctttggac cagaaggtgg attatcccgc gatgaagtcg
    28501 attattttgt agaacacaaa gggatacttt gtggattagg accaagaatc cttcgtacag
    28561 aaacagcacc tctttattta ttaagtgctg ctagctatca atgggaatta atgaatcctt
    28621 ctttcagcta acgttcttca ttaataaaag agtatcgaca gaaagaattg aaaaaatagt
    28681 caacaatttg tataatagaa acaattattt atacaatgga tacgaaaaat cgtttgaaaa
    28741 agcttttaaa aagcgaacat ttccgtaaaa aaacgtataa ctgcacggat tttgggacaa
    28801 ttatcaaaca gatatttgtc ccgattcccg ttttaccgaa aaaagaaagg gggatcgatt
    28861 cctgtgacaa aagaagaaat tttaacggga cccggtgtca taaagctcgt tagtcaatac
    28921 atgggtcccg aacatgtagc atttgttgag aaagcatgcg aatacgcgac tgctgcacat
    28981 gacggacagt ttagaaaatc aggcgaaccc tacattattc atcctatcca agtcgcaggt
    29041 atattagcag atttaaaaat ggatccccat acagtggcta caggcttctt acatgatgtt
    29101 gttgaagata cagaaatcac tttagaagat ctgagagaag aatttggcga tgacgttgct
    29161 atgttagtgg acggcgtaac caaattaggg aaaataaaat ataaatccca cgaagagcag
    29221 ctagcagaaa accaccgaaa gatgctgcta gcaatggctc aagatttacg agttatcatg
    29281 gtcaaattag ctgatagact acataacatg cgtacgttga agcacctgcg agaagataag
    29341 caaagaagga tcgctcagga aactttagaa atctatgcgc ctcttgcaca tcgtctaggg
    29401 atcagccgga tcaaatggga attagaagat acagcacttc gttatctaaa tccaaaacag
    29461 tattaccgta tcgtccactt gatgcagacg aagagagaag aacgtgaaaa atacgtaagt
    29521 ggtactgttg aagatattcg aatagcgaca gaagagctgg ggatttttgc agaaatctat
    29581 ggacggccaa aacacattta ttcaatctat cgtaaaatga aagatcagaa aaagcagttc
    29641 aacgaaattt atgacttgct agctattcga gtgatcgtag attcaatcaa ggattgttat
    29701 gctgtattag gagcaatcca cacaaaatgg aaaccaatgc ccggcagatt taaggattat
    29761 attgccatgc caaaagctaa tatgtaccaa tctttgcata ctaccgtgat cggtccggca
    29821 ggaaatccgg tagaaattca aatccgaaca caggaaatgc atgaaatcgc tgaattcggg
    29881 gttgctgcac actgggccta taaagaagga aaaaatgaaa aagtagaacc agatggtatg
    29941 acgaaacaat taagctggtt ccatgagata ctcgaacttc aagacgaaag ctatgatgct
    30001 tctgaattta tggaaggcgt aaaaggagat atctttagtg ataaagtcta cgtcttcaca
    30061 ccaaaaggag acgttactga gttaccaaaa ggatccggac cattggactt tgcatacagt
    30121 atccatacag atatcggtaa caaaaccact ggtgcaaaag taaatggcaa aatggtgcag
    30181 cttgattaca aattgaaaaa cggagatatc attgagatca tgacttctcc aaattcattt
    30241 ggcccaagtc gcgactggtt gaaattagtt gctactagca aagcaagaaa taagatcaaa
    30301 cgtttcttca aagcccaaga tcgagaagaa aatgtgatca aaggccacga atccgtggtc
    30361 aaatgtatta cagatctagg atttacgcct aaagatattt tgacgaagaa caaactgcaa
    30421 gaagcactcg atcgttttaa ttatcaaaca gaagatgatc tctatgcagc tgtagggtat
    30481 ggagaagtta gccccttgac gatggccaat cgtctgactg aaaaagaacg taaagaacaa
    30541 aaaatcgagc agcaaaagca agaagcagaa gaaatcatga atcagccgaa aaaagaacct
    30601 gacaaaatga aagtacgtca tgaaggtggc gttgtcattc aaggggtaga aaacttacta
    30661 attcgtatca gtcgctgctg taatccgatt cctggtgatg atatcgttgg ttatatcact
    30721 aaaggcagag ggatatccat tcatcgtcga gattgtccga atgttcagcc tgacaaacca
    30781 aatgtagcag aacgtttgat tgaagtcgaa tgggaagata catcgaatac acgaaaagag
    30841 tatgatgcag atttggaaat ttacggctat aatcgttcag gcttattgaa tgatgtactt
    30901 caaacagtca atgcgctaac gaaaaatctc aacagcgttg aagcacggac gaataaagat
    30961 aaaatggcga cgatccattt gacggttggt atccagaatt tatcccacct aaagagtatc
    31021 gtggataaaa tcaaagcagt acctgatgtc tacagtgtac gccggacgaa tggataggag
    31081 gacagatatg agagcagttg ttcaacgggt aagtaaagct gctgtgtcag tcgatcagca
    31141 agaaattgga aaaattggct acggcctact tgttttatta ggcattcatg aaaccgatac
    31201 gcaaaaagat gtggactatc tggtaaaaaa aattgcacaa atgcgtattt ttgaagatga
    31261 acaaggaaaa atgaatatta gtgtagaaga tgtgggagga caaattttaa gtatctctca
    31321 attcacactg tttgcggata cgaaaaaagg aaatcgacca agctttgtgg aagccgcacg
    31381 accagaaaca gctattcctt tatacgaatc attcaatgaa ggaatcagaa gtcgcggcat
    31441 cacagtcgct accggacaat tcggagcaga tatgtcggtt tctctcgtca atgatggacc
    31501 tgtaacgatc attattgatt ctaaaaacaa ataaagtaat ctggttttca ctagtaaaaa
    31561 ctatgaaaca cgtcgcttaa gttagactga taaactctaa cttaagtggc ttttttgtct
    31621 gcaaaaagcg cttcttttgt cagcagtcgg aaaaaaagtt aagcttaaat tatacaagtt
    31681 attttttata ggaggttgct gaagatgaaa gaagtaccag tagtagatat caaaatcaca
    31741 gatttaaaaa aattgtatca aaaaggatac aacatgttgg aggagttacg ccatgaagcg
    31801 gacgctcccg tcgtcaaagc aaagattttc aataaagaag ctatcacgat ctacggatca
    31861 tcagcagcaa aagtatttta tgatccaagg aatttcaaac gaaaaggtgc tatgccaaaa
    31921 ttagtattga agacactttt tggtcaaggt ggagtacaga ctttggatgg agctgctcat
    31981 caccatcgga aaaatatttt tatggatcta atgacaccgg aaagaatgga agattatcat
    32041 cgtatcttag ataagaatct gacacaagca cttgaagcac agcatggtca atttgaacta
    32101 tttgatttaa gtaagatggt tttctttaca tctatttgcg agtgggcagg tatcaattta
    32161 tcagcgattt ctaaagatga ggtcgaaaaa ttagcagaat atcaaatctc tatgatcagc
    32221 ggaacgttta cttctccgat tgatcacata aaaggtgtcg aaaatcgcaa aaaatcagaa
    32281 aaatgggcgc aaggcttgat tgaagaagct cgacaaaatc ctgtggctgg aaaagaaaat
    32341 gtggcattat atgcttttgc caatgccaca gatttagatg gtcagctttt acctctggaa
    32401 gtggcagctg ttgaattatt gaacatcatc cgtccaacgg ttgctctaac tgtgtgggcg
    32461 gcattgatgg gacatgcact tttcagtaga ccagatttgt atcagcaatt aaaaaatgac
    32521 ttttcgacgt tacaagatcc gtttattcaa gaaatgagac gctattatcc tttcttccca
    32581 atgctacctg cgatttcgct gaaagaagta gaagtagatg gttatcgtat accagaaggc
    32641 agttgggtta ttttagattt atatggaaca gaccatgatg aacgaacagt tgaagcgcca
    32701 gattccttta tgatcaaacg gtatgtaggg aaagcaaaag atatttccta taaagaagaa
    32761 tatgagatga ttgcacaagg cgggggaaat tttcgacaaa tgcatcgctg tgcaggagaa
    32821 tggatcactc ttcatagtct acgtgtgttt tccgatcagc tagtaaataa gtttgagttc
    32881 tctgtacctg aacaagattg gacgattcca ttcaatcagt ttccgactta tccgaatagc
    32941 cgagcgctac tttacaagaa ttagattagc ataaacttat cgaaaaaaag gaagatcaaa
    33001 cagtgctgat cttccttttt tcatattact ataaattgat aaggtcaatt gatcaaaatc
    33061 cacgaaatgc ttcatcaatg gtttgataat cttcattgga taaatgcaaa tctaaagctt
    33121 ggacattgtc agatacttgc tctggacgct tagctccagg tataactaca gacactcgtg
    33181 gattcttcat gtaccaagct aaaacaagtt gcgcaggagt ggcttggtat cttttagcaa
    33241 tggggcgaag tacatcgaca gcggttaaaa tttctctgaa acgttctcct tggaaatctg
    33301 gatttctttt tcgaggatct ccttcaccaa attgtttttc ctcaccaagt tcatattttc
    33361 ctgtcagcaa accggaagca agaggaaaat agggaacaaa actgattttg tttttttcaa
    33421 gataagggaa taattctttt tcagcctgcc gatggatcaa actgtattta tcttctacta
    33481 catcgacata tccatctgca ttcgcttctt tcaattgttc taacgtgaag tttgacacac
    33541 cgactgctcg gatttttcct gcttctttta attcgtgcaa tgtagcaact gattcatttt
    33601 ttggtgtgct ttcatccgga aagtggatat aaaaaatgtc gatgtaatca gtttgcagac
    33661 gttttagtgc atcttccacc gattgtttga gaaactcagg agaattatca aatgttcgcc
    33721 ctttatttgg tacatgagca gcttttgtag cgatgacgac acgtgagcga tcatattctt
    33781 tttccttcag tacctcgcca atcagttctt cggaacggcc gtttccgtaa gcataagcgg
    33841 tatcgatcaa attgatcccg ctattcaaag ctgtacgaac tacttgtttt cctgtctcat
    33901 cttccaaacc gtcaaaaaga ttatgtcctc caacggcatt tgctcctaat ccaagctgtt
    33961 ctgcgtagac ttgtgaatgt ccgattctta tttctgccat agctgattcc tcctttttgt
    34021 ctagtctaaa acaaaatacc aaaaagctga actaaaacgt ttttgtgagg ggaaatagtc
    34081 agatatctct tgactttgag actggttttt agtaggttat aagtaatatc gaatgtcaat
    34141 gaaagagcga gtaagttttt gaaactttac aagagaggat catcgaggct gaaaatgatc
    34201 taaagtttca ttagcctaaa gacactctgg agatggacga gtgaaaagcc gtcccgcgta
    34261 cgagcgttat tcgtaagagg aaagagaaat ctttcaaaat taggtggtac cgtgtatgaa
    34321 tttcattcac ccttgttcat gatgaacaag ggtgattttt ttatttacat agctaagaaa
    34381 gggagagaaa actatgagtt tccaaagacc aaaaggaaca aatgatattt tgccaggcga
    34441 atcagaaaaa tggcagtttg tcgaagaaac agcacgttta ttattcagag attatcagta
    34501 taatgaaatg cgtacaccga tttttgaaca ctatgaagtg atatcccgaa gtgtagggga
    34561 tacaacggat atcgtttcaa aagagatgta tgatttttat gacaaagggg aacgccatgt
    34621 gacgcttcga ccagaaggta cagcaccaat cgttcgttct tttgttgagc ataaattgtt
    34681 cggcccagag tatgcgaaac cgttcaaaac tttttatatg ggaccaatgt ttcgctacga
    34741 acgccctcaa aaaggacggt tacgtcaatt ccatcaaatc ggagtagaag catttggatc
    34801 agaaaaccct gcaactgatg tggagagcat ggtgatggca cttgatttct tcaaacagtt
    34861 ggggatcacg caaatccggc tagtcatcaa ttcattaggt gataaagaaa cgcgtcaagc
    34921 ctatcgtcaa gcattgatcg actatctttt accttttgaa tcagagctga gtgaagactc
    34981 taaacgccgc cttcatgaaa atccattacg cgtattggac agtaaagaca aacgagatca
    35041 aaaatttgtg gcagaagctc catctatctt agattatcta agcgagcctg ctcaaaaaca
    35101 ttttgatacc gttgtttcca tgctggatgc attggctgtt ccttatgaaa tcgacagtaa
    35161 tatggttcgc ggactagatt attacacaca tacgattttt gaaatcatga gtgacgcacc
    35221 aaaaatgggg gcacaagcaa cgatttgcgc aggcggacgt tatgacaatc tagtggaaga
    35281 actaggtggt ccttcaacac ctggcttcgg atttgcaatg ggtatcgagc gattactctt
    35341 gactatggag gcagaggaag tagttatccc tgcatttaat gagttggatg cttacgtggt
    35401 cgcactcgga gacgaaacaa atatcgaagc attgaaagtg gtccaagcga ttcgaaactt
    35461 tggtttttca gctgaccgcg acttcatgaa ccgtaaagca aaagcgcaat ttaagacagc
    35521 agataaagcg aatgccaaat tagtattggt gatcggtgaa gatgagcttg ctagtcaaac
    35581 gattaatgtc aaagcaatgg caagccgaaa agaaaaaagt tttccattag aagagatata
    35641 tgaacatttt gatgaagtct atgatgaaat gactttattc gagtaggagg aaaaaaataa
    35701 atggcacaaa gaacgatcta ttgcggatta gtttccgaac aacaattaga acaaacagtt
    35761 acattaaaag gatgggtaca aaaaagacga gaccttgggg gagtaatctt tatcgaccta
    35821 cgtgatcgtg aaggaatcgt tcaaatcgtc tttaatcctg aaatcaacaa agaagcatgg
    35881 gaaatcgcgg ataaatgtcg cagtgaatac gtgatcgaag tgactgggga agttagaaga
    35941 cgcggagaaa atgcagtaaa tcctaaaatg gccactggtg aattcgaagt gattgcaagt
    36001 gaaatcgtga ttttgaatca agcaaaaacc ccgccatttc aaattgaaga tgatcaagca
    36061 atcagtgacg aaatacgttt gaaatatcgt tatttggatc tgcgtcgtcc aaaaatgact
    36121 aaaaacatca aattaagaaa tgcgacaaca aaagcgattc gtcattattt agatgataat
    36181 gactttctcg atatcgaaac accttaccta gggaaatcta caccagaagg cgcaagagac
    36241 tacctagttc cttcacgtgt ccatgctgga catttctatg cgttgccgca atctcctcaa
    36301 ttgtttaaac aattgctgat gaatgctgga ttcgaccgtt attatcaaat cgttcgttgt
    36361 ttccgcgatg aagatttacg tggagaccgt cagcctgaat ttacgcaagt agatattgaa
    36421 acaacgttct taacagcaga agaaatccaa acttacacag agggattgat tgcgaaggta
    36481 atgaaagaag ttcgcggtat cgaagtcaca ttgccatttc ctcgtatgac ctatgatgaa
    36541 gcaatggctc gctacggcag cgacaaaccg gatacacgtt ttgatatgga attgatcgat
    36601 ctaagcgata cagtcaaaga agtagaattc aaagtcttcc aaatggctct tgaaaacggc
    36661 ggtgtcgtga aagcattgaa tgctaaaggc gctgcagatc gctactcacg aaaagacatg
    36721 gatcagttag gtcaatatgt aggacaattc ggagctaagg ggcttgcatg gctgaaagta
    36781 gaagaagacg gattgaaagg tccaattgct aaattcatgg gagaagcaac agaagcaatc
    36841 attaaagcaa cagatgcaaa accaggtgac ttgctgatgt ttggcgcaga taaaagtgaa
    36901 atcgtcgctg ctgcattagg tgctatccgg acacgtttag gaaaagaatt aggactaatt
    36961 gatgaatcga aattcaattt cttatgggta actgattggc cacaatttga attttcagaa
    37021 gaagaaggtc gatatgtctc tgctcaccat ccattcacga tgccaaaaga agaagatatc
    37081 ccgttattgg ctgaagaccc tgcaaaagtc tatgcagaag cttatgatat cgtcttgaac
    37141 ggttatgaac taggcggagg ttctatccgt attcatactc gtgagcttca agaaaaagta
    37201 ctagaaactc taggctttac aaaagaacaa atggaagaac aatttggttt cttgttaaca
    37261 gctttagatt acggattccc tccacatggc ggtattgcat taggattgga ccgttttgca
    37321 atgttattag ccggagaaga taatattcgt gaagtcattg cctttcctaa aaacggaaaa
    37381 gcagcagatg tgatgagtga tgcaccaagt atggtttcgc ctttacaatt gttcgaattg
    37441 aatattgatg taacgaatat agaagaatag ccgataaaaa ggtaaaaagg atttgtcgaa
    37501 tacgcttctt ttgacaacct tgatttaatt gaaaaacact ttataatgaa gccataacat
    37561 ataaagcggg gcttttccat gaaaaaaaca ttcgaaaatt gggcttatca tgattacacg
    37621 accaagcttt cggtatctat cgtttatgcg ttgatcgcat cagtcgcatt gaatttcttt
    37681 tatcagccag gacatattta ttcaagtggt attactggtc tagctcagat tttgacgact
    37741 ttgtcaaaat cactgatagg attcgaagta ccggtatcaa caactttgta tttactgaat
    37801 atacctttgt ttgtattggc gtggttaaaa atcgggaaga agtttacaat tttcactttt
    37861 atcacagtca ctttgacctc cctttgtatc catattttac cggaaacagt cctatctacc
    37921 gatccaatca tctgtgcgtt gtttgggggg gctgtgatgg gactaggtat tgggctagcg
    37981 ttgagaaacg gcctatcatc gggcggtcta gatatcgtga gtatcacgat tcgtaaaaaa
    38041 acaggaaagt cgattggttc tatttcgatc tacttcaatg cattgatcgt ttttgttgcc
    38101 ggaattctat ttggctggca atatatgttt tacagtacgt tgtctatttt cgtcagtgga
    38161 aaagtaactg atgtcgttta tactaaacaa aagaagatgc aggtaatgat cgtaacgaaa
    38221 catccagaaa aagtagtaaa tgaaatccaa aactgtatgc gtagagggat cacgatcatc
    38281 aatgaagcag aaggtgctta taagcatgat aagcagacag tattgatgac gatcgttaca
    38341 cgatacgaac tgcctctact tagagaagca atgaaagctt ctgatccaca agcttttgta
    38401 agtattgcag aaaacgtcca gatacttggt cgtttctatg aggatggcat ttagatcgag
    38461 tttgacaaaa attcatgaaa tactgctgta gacagacgtt cgtttgtctg cagttttttt
    38521 ctaccatagt agaatcagca aagaaaaact gcttttttat ttgaaatttt cttaaagttg
    38581 aaaaaaagca tgtttatttt tttaaattta tgggatgata gaaaaaaata gtagagaaag
    38641 gacacgattc tacttgctat tttcacttaa tacccgtata ttacgaatga aaaaagttta
    38701 tgaaaagttg gaatattgaa tggaaaaaca aaaaaggtta aaaaacagcc gctacatcac
    38761 tgggtttgat gggctccgtt cattagctgt ggtgggtgtt atcttctatc accttctgcc
    38821 aactagtatg aaaggcggct atttgggcgt tccgattttt ttcgtcgtat ctggttattt
    38881 gatcacggat ctgctgcggc aggaatggga acaaaatgga aaaatcaata tttggcaatt
    38941 ttatgtcaga agaatgaaga gactctatcc ggcacttgtt tttatgcttg tgacagcttc
    39001 ggcttatatc actttgtttc agcgagggct gttaaacaat cttcgaggag ttgtggtcag
    39061 cagcttactg tatgtcaaca actggtggca aatcaataac ggtttatctt atttcgatcg
    39121 atttgctaat gagtcaccgt ttacgcatat ctggtcttta gcagtagaag gtcaaaacta
    39181 tttggtttgg ccgatcttgt ttgtattgtt gatggtattt gttcgtaaaa gaaaatggat
    39241 cttttatact gtgttaggta catccttcct ctccgccatc ttgatgatga tcttgtatac
    39301 accaggaggc gatccaaccc gtgtttatta cggtaccgac actcgtctgt tttctatatg
    39361 gttgggaagt gcgcttgcgt ttgtttggcc aagtacgcgt ttaaagaaaa acatcccgaa
    39421 acaagcgaaa aatattctga atatcgctgg aggaatctcg cttttggcac tgattctttg
    39481 cttcttcttt ttagatgatc attacaattt catctattac ggaggaatgc tcctagtgag
    39541 tctgttatgc gtagtgctag ttgcagtgac agcacacccc ggagcaagtt tgaatcgatg
    39601 gctgacaaat ccagtattta cttggattgg caaacgaagc tatggcattt atctgtatca
    39661 gtttccagtt atgatttttt atgaagcaaa ggtgactgat attgccgatc atgttttgtt
    39721 gcatacttta gtggaaatca cattgattct aatcatcagt gaactttctt atcgatatgt
    39781 ggagcgtccg ctagcacgtt ttgattatgg tcgtacattc caagtgatga aaggatggtt
    39841 cagcaaaccg atcatcagta agagcaaacc atgggttgtt ccaggcacat tacttgtagc
    39901 aattgcactc gttggtttcg ttacggcacc gaaaaacgct gtaacagccg atcaaaaaca
    39961 actgcaggca aaaatcgctg aaagtaaaaa attagcagaa gaaaccagaa acaattcaga
    40021 aaacaccact agttcggttg accaagcgat ccttgataag tacgagttgt ccaaggcaca
    40081 agggaataag gcacaaaaat tagaactaac ggcatttggt gattcagtta tgttggatgc
    40141 tgcaacggat ctacaagaac tttatccaaa agtagtggta gatggagatg tcggacgaca
    40201 attatatgcc agtgtaccgt acattgaaaa attaaaggaa caagatttgt tgaaagacac
    40261 tgttctgatc ggactaggaa cgaatggctc attctcggaa gctcagtttg atagtgtgat
    40321 ggatgcaatc ggtgatcgaa aagtatactg ggtcaatgtc cgagtaccga cgaagcgctg
    40381 gcaaaatgac gtgaatgcga tgcttgaaca gatgacgaaa aaatacgaca atatgacgct
    40441 gattgattgg tacagctaca gtaatgacca tagtgactgg ttttatgatg atcaagtcca
    40501 tccaaatcct gaagggatga cacattacat tcacttagtc agcgaagcct tgctaggagg
    40561 aagttcaagt acgaacagca gtgagacaaa cgtgacagaa tgatagctac aaaaaacttc
    40621 aggcaaaaac atttgttttt gcctgaagtt tttttatatt taccgtgttt ttaacattca
    40681 tatagattca ggaagcaaaa cggttgagtg tgtctgtaaa taacgttctt tttgttttcg
    40741 ggtgagtttt ttgaatgcag cttctgcttt tgttgcttcg cttcttgaag aaaatacttc
    40801 tgcatggatc atcttaacag gtcttctttt ttgaagacga gtatatttag caccagtacc
    40861 attgttatgt tcctctaatc gtctagaaag atcggttgta tatccaccat aaaatgtctg
    40921 atctttacag aataatacgt aaaaataatg ttcatttgcc atagagcatc tcacgaatct
    40981 ctgcagtgta ttctccatcc atattgtaag tgatcaatgg tggagcgact ttgaatccgt
    41041 ctaatttgcc ttctttgatt ccttcaacta acaaaatatt tgcctctttt ccaggttttg
    41101 ggtaaacaaa ttggatcctt ttaggtgcaa ttcgatagtc ttccatgacc tgaatgattt
    41161 ctaagaaccg atcaggacga tggaccatag taaagcgtcc atttgttttt agtagcttac
    41221 ttgaaatatc gatcacttct tctaacgtcg tatgaatctc atgacgggcg attgccaggt
    41281 gtttattagg gttcgtttta tttgttggca gccctttgaa ataaggtgga ttacagacga
    41341 ctaagtcaca agaattatgt cgaacgacag ataagctgtc tttcagatcg atggtgtgga
    41401 cagtcatttg ttcatttaaa tggttaagcg cgatactacg tcttgccatg tctgctagac
    41461 gaggttgaag ttcgatagcg tcgatagccg cctgggttcg tttacttaaa aaaagtccaa
    41521 ctgcgccatt tcctgcacaa aggtcaacaa tcttcccttt tttaggtaca cgggaaaagt
    41581 gagcaagcag cacagcatct aaagaaaaag aaaacacttt ggaactttgg ataatcttga
    41641 tatcttctgc gtataattgg tcgattcgtt cgtcttcgtg aagcatggat tccctccaaa
    41701 atgaaaaaaa tagtcgtctg tattatacta tttttattaa aaagaaccaa ggtggcttgt
    41761 caaacctcta ttttttatgc atactatgac cataagaagg gaaaggaaga gactatgttt
    41821 tatcgattta tgcgcggggt agttcgagta ctattagcct tagttaatgg aaatgcccgc
    41881 tatgagaata aagaagtttt accgcaagat gagaattaca tactagttgc tccccatcgt
    41941 acttggtggg acccattata tctggcggta gctgcagcac ccaaggaatt cagctttatg
    42001 gcaaaagaag aactgttcaa aaatccagtt ctgcgattta tattaactcg atccaatgct
    42061 tttccagtta aaagagaaaa tccaggacca agcgtcatta aaacacctgt aaagattttg
    42121 aaatcgacaa atttgagttt gatcatgttt cccagtggga caagacatgc tacagaactt
    42181 aaaggcggaa tggcattgat ttccagaatg tctaaagcga aaatcgtgcc agctgtatat
    42241 caaggtccgt tgacacttgg ggatctgttc aagcgtcaga gagtgacagt gcgtttcggc
    42301 gaaccaatcg acttatcaga tatcaagaaa acggataaag aaggtatggc ggagatcgaa
    42361 cgacgaacac aagcagcttt tgacaaattg gacaaagaag tgaaccctga tttcaaatat
    42421 gaagtcaaaa aaaaataaaa tgataagttt atgcgtgggc ggtcttttga atctactgtc
    42481 ctgttcagtt cagacttttt caatgaaaac gagggagaga cgtcaagtct gccctctttt
    42541 tgcgttccaa tatgtaaaat agaagacagg tgtcatgtaa tggatcaaag tgttataata
    42601 attagaagga cattttttga ggtgattgaa tgcgtttttt acatacagcc gattggcata
    42661 tcggaaaaaa actacaaggt tatgacttga ttgaagacca gcgtcatgta ctatcttcta
    42721 tactagatat cgcgaaagaa gaaaaagtag aggcaatcgt gattgccgga gacttatatg
    42781 ataggagtgt cccagctgtt gatgccgtag aattatacaa tgaattgatg gtcgattgga
    42841 atttaaagga aaagatcccg atttttgcta tatcaggaaa tcacgatagc agtacgagac
    42901 tttcagcggg tacgccatgg ttcagttatt cgaactattt tttgcgaaca cagctagcag
    42961 aagcatttga accagtcgag tttaaaaacg tacaattttt ctttcttcct tactttgaac
    43021 caattgcagc gagattatat tttgaagatg acaatatccg aacgatcaag caagcggtag
    43081 ccaaggtgat agataaaatg caggagctat ttgattcaga aaaaaaacaa gtattgatta
    43141 gccatttttt tgtaatggga agtgtaaaat ctgattcgga aacgaaaatc gaagtaggcg
    43201 ggttggattc gatccctggt gaactactta caccgtttga ttatgtagca ctaggacatt
    43261 tgcataatca agctgctttg agacaggata atgctcgcta tagcggttcc ccattgaaat
    43321 tctctttatc tgagatcaac caaaaaaaag gcgtatggat cgttgacctg acaacaggcc
    43381 tggagttgaa ttttagggag atcgaacccc tgcatgatat tgaagaaata acagcgagtt
    43441 tcaaagaatt gctggatcct gccttttatc agtccgtcaa tcgggagaat tacatctata
    43501 tctatctcac tgatagaacg atcatcccga atatgatgaa ccagcttcgg caaatatatc
    43561 ctcgtatttt ggggattgaa cgggcaaacg gtcgagcaga acaaacaaag cgcaaacgaa
    43621 aagtactaga aatgaaagat cctcaaaaat tagcagaagc attttttagt gaaatgacgg
    43681 aagagcagct aactgcaaac caagaaaaat ggctcggaga aacgttgcaa gaaatcatgg
    43741 aaatggaatg atcaagaatg aaaccgaaaa aattacaact taaaaatttt ggtcctttta
    43801 tcaatgagac cgtagatttt tcacgtttga cagaagcacc gctttttttg atcagcggga
    43861 agacaggagc tggcaaaacg acgatttttg atgggatcac cttcgcactt tttggtgaaa
    43921 cttccggaag acttcgctct ggaaaagaga tgcgttcttt gtttgctaca ccagacgaag
    43981 aaacaagcgt gactttttct tttgaacatc agcagatgag ctatgaaatc gaacgaaaac
    44041 cagaacaagt tttagcaaaa cgaaaaggaa acggaacaaa aaaacaggca gccaaagtaa
    44101 cgttgaccat ttttgatggg aaaggaaaag aaataaaaca aatcacaaaa cgtacagaag
    44161 cagatcagtt gatcagagaa ctgatgaatc tcgatgcgaa acagttttct caaatcgttt
    44221 tactacccca aggagaattc cgtaattttt tggtttcatc aagcagtgat aaagagactg
    44281 ttttacgtca tttattcggt actcaatttt tccaacagtt caatgagcga ttaaaagaaa
    44341 aagcaaaaca acagcaacaa aaattggatc atttagagca agagctgcat cttctgcaaa
    44401 aaaggtttgt tttagtcaaa gaagaggaac cagcagcagg gtttgaagcc gttttagccc
    44461 aatggacaaa ccatcaggca atactcgaaa aacaaatcac ggaagaaaaa gaagctctaa
    44521 cggaacttca ggaaaaacaa aagcaactag aaaattccta ctatcgtttt gaaagcttga
    44581 aaaaaagtta tgaagaaaaa acgatattaa taaacaagca aaaggaatta gctgctcaaa
    44641 aagacgaaat agaggaaaag aaacgatgga ttgggtatta tgaatttacg caacgattag
    44701 tcgaaccaat ccagcgccta caagaaataa aagttgaaaa aatagaactg tcacaaaaaa
    44761 gtagtgagca aacaaagaaa ctagcgatga ctacgcagaa ttatgaagag tggcaaaaaa
    44821 atgaagcaaa ccgcgtacgc cgacaaacaa aaatcaaaga agctgctcaa aaactgcaaa
    44881 acaaacagat gatggtgcca attgtcgaat ccttacgtag taaaaaacaa gacgcagaac
    44941 aattaacaat ttcattggat aaagaagaat tgaatgccga gaagctgtat caaaatctcg
    45001 ctatctatca ggaaagaact cgaattcttc aagaaaattt actggatcag gaaaaactgc
    45061 aagaagatcg tcttgctttt ggacagttaa aatatctaga tgaaaagcag caagaaatag
    45121 aaaaacaaaa agcaaaagac actgggtcgc tcgcacagat aagagaagaa ctcaaaaaag
    45181 caactgaaga aataacaaaa aatagtcagc tattgaaaga acagacagaa caagcagaac
    45241 agctgaaaag ccaatgggca aagcagcaaa ttgctcgtct tcagcttttt ttgcgtccag
    45301 gggagccatg tcttgtttgt ggatcaacgg aacaccccat acaacaatta aaacacgaac
    45361 agccaaataa ggaagatatc ttaaaagtag aacaacaact ggcagaagct gagtctgctg
    45421 tagaaagaac tcagcaaatc attgccaaaa gtgaagcaca tgaaaataat cttcaacaac
    45481 aattatcaga tttagaacaa aaaatcatgg caacgaatat acaattggct gaacaagaag
    45541 aacacttgca acaggctttg aaaaaggcat ttcctgagat aacagaacaa aaaacaaaaa
    45601 caactcttgt agtaatcgaa caatctttag atagacgaaa aagcaagatg gccaatgcaa
    45661 gaatagagct ggaagaaata agacaacaag cagaaaagct cgaacaacaa ttagctgtct
    45721 acaaacagca gcagacagaa aaaaataatc agctccagca actgcaagga gaaatcaaaa
    45781 gtttagaaca acaattgcaa gatacggata gtaactgttt agaagaggag atacgacgac
    45841 ttgaaaaaca gttagaagaa gactcgaatc aactagctgt tgagcagaaa gcaggagtgc
    45901 aattgttgca agatcttcat acgcttcgga cacagcttga tcttcaacat gaacaaatag
    45961 aaaaaataga tgaaaaaata gaaaaggaac aagaaagaat agctaaaaag ttagaaagcc
    46021 agtcgtattt ttcaacaatt gaagaaatct gtgcatttgc acaacagcaa gcacaatata
    46081 aagaaaacca acgtacagtt acttcttatc atgaagagca attagtgaca gctgaccggc
    46141 tgcgtcaatt agaaaatatc cagctaactg agacattccc agatacagaa gagatcagcc
    46201 aacagttaga agaaataaga cgtatgatcg aaataaagca agaaaagata tacggtttac
    46261 aagaacaaaa aattagtaat caaaaaatct acgatgaatg tctttcgatt tatcacacaa
    46321 gccagacgaa attagatgag cttacgcaat tacagcagct ttcgcaaaca ttcaacggag
    46381 aaaatcctaa gaagactagt ttggaacggt atgtgcttca ggtttatctt caagaagtgt
    46441 tacaagtagc aaatgatcat ctccaacgtt taacgaaaaa tcgttatcaa tttgagctag
    46501 cggataccat tggcagttat cgagggaaaa ccggattaga aatcaatatc tatgatgatc
    46561 aggcaggtat gtctcgaagt gcgcataccc tgtctggcgg tgagagtttt attgcagcac
    46621 tttctttagc attagctctt gccgatgtga tccagacaca agctggtggt gtagcaatcg
    46681 atgcattgtt tattgatgaa ggatttgggt cattagatga agaagctttg gaaatggcaa
    46741 tggaagcttt agaaacgatc gaaagtgaag gaagaatgat cggcatcatt agtcatgtaa
    46801 gagaattgaa agaacgagta ttacaacaaa taagaattga tacagaaggt agcggacaaa
    46861 gcaaaatacg ctatcaactt ggataaaaag ggagattggt aactatgaaa tggagtattc
    46921 cagaaaaagt cattgcacgt ggacgtcaat acttgcaaga aggccgagtg ttatcggttg
    46981 tccctgacca agaagaaaac gtgtggcatg cagaagtctt gggcagtgaa ttgtatatgg
    47041 ttgatttgga tggtacagca aaagagatag attactgcca atgtccctat tgggaggaac
    47101 ataaatattg taagcataca gttgctgtgg aactttattt acggtcgaaa aatctatctc
    47161 gtatcatgaa aaaagaccag ccaatgctag aaccggtaaa agcaacaagt gagggagaaa
    47221 tcattgcaaa aggtttttct cgtctaaaaa cacaaaacaa tcctcaagcg gtgcaacctt
    47281 tgattattga ataccaagta gaaacgattg agactaatca gtatcaccca gagctttcta
    47341 ttttggcact ttacttgcgt atcggttcat tggcatcacc gaaaaaaaca tatatcgtaa
    47401 aaaacatcta tgatttttta caagcttaca cagaaaaaga aacttacact gttaataaac
    47461 agtaccagtt tcgtctagat aaacgggcgt ttcaaaaaga agatcaaaaa atactcacgc
    47521 atcttgcagg aagtgctcag acgcatcaat tattaggtac gaacggctta caagtcaaag
    47581 gaaaactaga taaaagatat ctgcttctcc cagtagaaca agcccgcttc ctgttagagc
    47641 agatgaatca gactacacgt ttgcatctgc agataaatga acaaaacgat caaggaatac
    47701 aattttccga tcaaaacaaa cctttatcat tcaaagtgga aaaatttgga gaagattatt
    47761 tgctgcaagc attgaatgat tttgattttt tctttatgca ttatcagtgg ggagttatcc
    47821 aagggacatt ttattctctg acgaaatatc aacaaacggt ttatcaaact tggaaacaat
    47881 tgattcgccg attggagaaa ccagaagtag tgttcaagaa aaaagaatta ccgtctttgt
    47941 ttaaagagat tatccctctg ctgtcagaga tcggcgacgt agctattgcc cctgcagtag
    48001 aagaggaagt agcagatatc cccgtcgaat ttattttctt tttccgaaaa gcaaaaggaa
    48061 aaatccaagc tcgagtcgat ttcgtctatg gcgaagtcat ctattcgact gacccgaagc
    48121 atgaggtctc gacagaacag gcttttcagg tgttacgtga cacagtagaa gagaaacgag
    48181 tacttgatct attcagaatg tatcgctacc agaaaaatga aactggttat gaacgtccat
    48241 tgccaagcgg ggaagaacta tatgcctttt tccgcacaga actatctgtc ttccgtcagt
    48301 ttggtgaagt cagacttgga agaaaattgc gagagttgta tttagatgcg cataaacatc
    48361 aaccgaaaat cgaagtaacc gagaccggtt catggctcga tattcgattc gatgtgacag
    48421 ggatagagga gcaagaaatc gatcatgtcc tgcaaagctt gctgagaaat gatgcttttt
    48481 atacattaga aaatggtcag gttctttctt ttgattcaga agaatttcaa caaacaagcc
    48541 aagtgttgca acaattacgg gaatcgatac gtacagaaga aggaacgatc catgtaccta
    48601 aaaatcaagg attgatcatt caaaatcaat tggaaaaaag caatgctact tttagtgaat
    48661 cgttccaaac gatggtacaa gatctcattc atccagaaag ataccaggca cagcttccca
    48721 aaggattgaa tgcaacgatg agagattatc aaaagcaagg ttttcgatgg ttgaagatgt
    48781 taggtcatta tcagtttggc ggtattttag ccgatgaaat gggacttggg aaaacactac
    48841 agacaattgc ctttttactt tcggaaaaag aagaacggaa aagcttctct gctttgatcg
    48901 tagcgcctgc gagcttaatt tacaactggc aggcagaggt gagaaaattc gccccttctt
    48961 taagtatcca agtaatcaat gggaataaaa aagaacgtga agaattactg gctaaagaca
    49021 cagatatccg agtgacttct tatgccagct taagacagga tctagctaat tatcagtcgc
    49081 aaaaaattga ttatttgatt cttgatgagg cacaaatggt caaaaatagc agtacgaaaa
    49141 ctgcacaagc attgagagaa ttagctgtac ctcagcggtt tgcactgagt gggacaccaa
    49201 tcgagaataa tttagaagaa ttgtggtcat tgtttgcaac gattatgcca ggatttttcc
    49261 cgactaaaac aaaattcagg gaacttgccg cagaagaaat tgcgcaaatg attcgaccgt
    49321 ttattttacg gcgagataaa caaactgtgt tgaaagactt gccggaaaaa acagagatga
    49381 atctatattc tgcacttaca gaagaacaaa aaactgttta tttagcttac ttgcgtcaaa
    49441 tgcgtgaaga aatcacatca atggattctg aagcattcaa gaaaaaccgg atcgggatac
    49501 ttgctggtct gacaagactg cgacaaattt gctgcgatcc tcgtctgttt attgaagatt
    49561 accaaggcgg ttccggcaag ctcgaacaag taaaagatct actggtagct gccaaagaga
    49621 acaaacgaag agttttgctg ttttctcaat ttacagggat gctgactatc ttgcaagaag
    49681 agttagccga gctagggatc tcgacatttt atttaagagg cagtacgaaa ccacaagatc
    49741 ggttaagtat ggtagacgca tttaatgctg gcgaaaaaga tgtttttctg atttccttaa
    49801 aggctggggg aactggattg aatctcacgg gagcagatac ggtcatcttg tatgaccttt
    49861 ggtggaatcc tgccattgaa gaacaagctg ctggacgtgc acatcggatc ggacaaaaaa
    49921 atgtggtaga agtgtggcgt atgatctctg aaggaacgat tgaggaacgc atggattcac
    49981 tccagcaaga aaaacgcgaa ctgttccaaa aagtgatcca aggtaatgaa gaacagttga
    50041 cgaaactgac tgaagaagac atccgcatga ttttaagtgt aggagaaatc gaggagtaag
    50101 agactcgaaa aagcgttcag taatgggtaa gaaagccttt ctaaagaagc gattgtttat
    50161 ttgatttaaa aaatagcaag gaggctggga ttccatttta tcccagcttc cttgctatca
    50221 tttttttatt tctattttta aaggttatac aaagattgtc ttcattgtct ctaacagctg
    50281 atcaaaagaa gaaatctcga tatcgcaaat tggagtctgt tcaattttgc gttctcgatg
    50341 gttgaaccaa agcgcttgcc agttagcact ttttgcacct agaacatcat tgtcgtaatt
    50401 atcgccaacg tataaggtct cttctggaag catatgaaat tctttctcag ctagctgaaa
    50461 aatctctttt tctggttttt ggaaacctgt tgcttgagaa atgagcatat tgcgggaagg
    50521 aatccaattg ttcaattgaa gttgcttcag tttttttgtc tgatggtcag tcggcccatt
    50581 tgtgataatt cctaatggga cattcttttc cttcaagaaa tcgagggttt ttttgacctc
    50641 ctcatgcaag cagatattat caagttcttc ctcgtaaatt ttttgaaata agaggccatc
    50701 ttcttccgta atatgagggt agtctaaatc ttttagtgat tggctgatcc gatgtgctcg
    50761 catatattct agtgtccaat ctcctgccat tacttttggg aagttttcat cactatgatg
    50821 gcggaaccgg atatatagtg gatgcatatc tgcatctgaa acaagcggaa cgactctttt
    50881 gaccgcgttt cgaaatggct gctgctgatc atacatcgta tcatcaacat caaacacaac
    50941 tgatttaatc aaaattttac acgcctttcg gttttcattg attttttcac aaatccttga
    51001 ctattgtaac agaaaaaatt gtctaaaaaa gctgataaat aaaggttttt cttactatat
    51061 ttctatttcc ttgacagaat aaggttttta tatttcagtt tttttcacta aaaatcgtga
    51121 aacacgtcaa taagtttgat gaacaaatta atcattatgg tagaagtcga ttgatttatt
    51181 ttaatgcaat ctctgtggaa ttttcgaaag atttattttt ttaatacagt ctcaatataa
    51241 ctattcaaac ataaaataac gttatttgat ttatttgttt atagtaaatt ttatttagta
    51301 atcattgctt taaaatacta cgcttatata acagagaaaa taagcgttat aataaagaac
    51361 agtattattt aaaatgacag aaaacattac tattaaagaa ataaaaaaca taaaaaatct
    51421 taagaaatcg gtttcattta gcttttgaga taaaataata gtacagtttt atacgctgtt
    51481 tcatatgttt agaaaatttg aattagattt gaaaactcta atttttaaat gataaaaaaa
    51541 gcgagaatgc tggttttact gtatattaac cttagataac tttcattttt ttaaaaaaat
    51601 tatgaaaaaa aggtagctat ttaaaagttg attatgtata atagaaacag atgggagatt
    51661 attatgatct tctgttacaa aaaagtgatt cttatcacga aaaggagtgg tgcacgttgt
    51721 taacattata tacttcccca agttgtactt cctgcagaaa ggctcgtgcg tggttgcaag
    51781 aacatcagat tccatttgtt gaacgtaata ttttttcaga accattgaac agttctgaat
    51841 taaaagcgat tttacaaatg acagaagacg gaacagaaga aatcatctcg acacgttcca
    51901 aagtattcca aaaactgaac atggatttag atgatctgcc gttacaagaa ttattggaac
    51961 ttgtgcaaaa caatcctgga ttgcttcgcc gtccaatcat gattgatgat aaacgtctgc
    52021 aagttggttt taatgaagat gaaattcgtc ggttcctacc gcgcgatgta cgacaattag
    52081 aattacgtca agcacaattg atggctggat tatagaagga taatacgaaa cttggaaaag
    52141 actattacaa agatagtttt tccaagtttt tttgttttta caaaaagtat tcttgattag
    52201 atgtataaaa aggtagtaga agatgatgat tgcacgaaaa atagagaggg aaaattagct
    52261 gaaaagtcgg ctatatagta ttcgtctgcc aaaaatgtta tagttaaata agatagcttg
    52321 aaaggttgaa aaatccgctg gttttattaa attcgcttaa cagcgagatt ttttttcttt
    52381 acatttaagc tgatttcgct acaatgaatc tatattaaac attgtggcaa agtgaggtgt
    52441 agcaaaatgg aaatggaaca tattaatgaa aatacgatcc gtgtactaat tggtcatgaa
    52501 gatttagagg aacggggcgt ttcatttttg gatttacttg gcaatcacaa agaaatcgaa
    52561 aatttctttt atagtatttt ggaagaagtt gatatcgaag aggagttccg aggaagcgaa
    52621 gctgtcactt tccaagtttt gccaaaagga gacggattag aactgtttat cagtaaaaat
    52681 cttccggatg aagaaatttc tcaatcagaa gattctactg ttgattcaga agatgtgagt
    52741 gactttcttc gtcagcagat tattggcagc gatccggagg aagaattatc tggaagcgcc
    52801 ctagaagaac aggaaagaca agcaactttt gcttttgaag attttgaaca agtgatccaa
    52861 cttgcaacag atgtcgaact agagtctgct tggactgatt tgtatttatt cgaagagcgc
    52921 tattacctag tcgttcactt ctggatggaa aatctcaacc aagcggatgt agagaatcag
    52981 attgctcgaa ttttggagtt tgctgaaaag agcgatcgta caccagaaac attgaatgaa
    53041 tatggacaat gtctgatggc gcgtaatgct atcgagcgaa cgaaatttta ttttgtataa
    53101 atcagcatta tagtaagaga ctgtgacaaa aattgtgtcg cagtcttttt ttcgtataaa
    53161 tgtgttcaaa agcttgcatc tgcggtaaca agcccaacca aacgaaaaat attgggaact
    53221 atttttgttt agctgttctt attacttgat gcagaatagc tttttcacgc cctctttttt
    53281 cgtgtaaata aattcttaga cgtaaataag ttcagaggga gaaaaatgct gcttgcaatg
    53341 aatgaatata ataagtgctg ttttgctaaa catgcagaaa aaggaaagaa atattattgt
    53401 cgtggctgtc gaaaaagagt gattttgaaa aagggaaaga aaaaatgtgc tcattttgcc
    53461 catcaaaaat cagataactg ttcggtcttt agtgaaagag aatcaaaaga gcacttacag
    53521 ttaaaggaat gcttcatgga ttggctggga cagtcagtag aaccagtatt tttggaagcc
    53581 tatttgccga ggttgcggca aaggccggat attttgttgg cgaatctagc aatagaaatc
    53641 caatgttcta gactgtctca ccagagattt attgaaagaa cccaaaatta tttgaataac
    53701 agttaccaag tatggtggat acttggtaat tcttttcttg gacaatcgca attctcattg
    53761 atagaaaaaa gttgctgcta ctataataga aaaagaggag tgcattgctg gaaagctgat
    53821 ctcaagaaac aaaagcttta tttgtatcat catattacag agactgtatc agggcatatt
    53881 tccttttgca gtagctgctg gactttttct agtagggacc ttaaagaaat attcactagt
    53941 aacgaaatta aaatgaacca aatgaaaaaa atagaaaaag tgagtgaaga tgggaagaaa
    54001 tggctgacac gccaacttat tcacaagcaa aagaatacag tatccataca agaacaatgc
    54061 tacttgcgtc ataaacatct tttgcatcta tcatcgtgga tctatcaaaa aagtcggttt
    54121 ttcttttatt tacaagaaca ggtttttctt tatcgaatgt tgtacgaaga agccttggaa
    54181 aaccagaaag ttcctgattt caactcatgg ctttgccaaa taaaagaaca taaaagggaa
    54241 tggctttttc ctatgattga tgaagagatg gtctatcagc agttttttaa cgaatgcata
    54301 cacttgtctt ctttgaaaaa atagtaagta taaaaatagg ctaggaatcg gttttctatg
    54361 taaaacaaga tttagttatg ctaaaattga tggaagcaga cacatgaatg agtagaaaag
    54421 ttagatgctg tgacatgtgg atgttagact atgatctatt ttgatagata gagctaaggg
    54481 aagaaaagga gtggatttca tggaagtaaa gcagttgcca aaacgagaag aattgcctga
    54541 aaatttaact tgggacttga ccaagatctt ttcaagcgac caagagtttg atgagaaata
    54601 tttggaatta tcagaagagt taaaacaatc tgaaaaacac aaaggaacac ttgatcaagg
    54661 cgcttctcaa tttttaaatg cgattgaatt cgtattgagg gtttatcgcc aaactgaagt
    54721 catttatgta tatgcgcacc ttaaaaacga tcaagacact ggaaatacag attaccaagc
    54781 gctttatgca agagcaagca gtctgttttc gaaagttagt gaagccgttt cctggtttga
    54841 accagaaata ttgcaattgt cagatgacca gatttggcaa tatttcaaag aagaaccaaa
    54901 attggaagtc tatcgccatt atatccagca aatagtagat aatcgagccc atgtcttatc
    54961 tgctgagcag gaatctcttc ttgctggagc aggtgaaatc tttgatgctt caagtgatac
    55021 atttgctgtt ttgaataatg cagatctagt ttttccaacg attgaaggag aaaatggtga
    55081 aatagtccaa ttatctcatg gcgtgtatgg tcagttgcta gaaagcacgg atcgaagggt
    55141 gcgcgaagca gcatttaagg ggttgtacag tgtttacgaa caatttagaa atacatttgc
    55201 ttctacttta ggcacacata taaaaggaca taattttaaa gcgaaagtcc gtaattacag
    55261 ctctgccaga gaagcgtctt tgagcaataa tcatattcct gaaagtgtat acgatacttt
    55321 ggtagacgtg gtaaacaagc atttgccttt gttacatcga tacatggaat tacggaaacg
    55381 tttattagaa gtggaaaaac tgcacatgta tgatctttat acaccggtct taggggaagc
    55441 tccaattacc tttacgtacg aagaagcaaa agaaaaagct ttagaagcac tgaaaccaat
    55501 gggtgaagaa tacatggcca tcgtagaaaa agcattctct gaacgttgga tcgatgttgt
    55561 cgaaaataaa gggaaacgaa gcggtgctta ttcttcggga agctatgaca caaatccata
    55621 tattttattg aattggcatg atacgctgga tcagctattt acgcttgtcc acgaaatggg
    55681 acatagtgtt catagttatt tcactcgttc gaaccagcct tatgtgtacg gcgactactc
    55741 catcttttta gcagaaattg cttcgacaac gaatgaaaat atcctaacgg agtatttatt
    55801 ggaaacagaa aaagaccctc gtgtacgggc ttatgtactc aaccattact tggatgggtt
    55861 taaaggaaca gttttccgcc agacacaatt cgctgaattc gagcatttca tgcatacgga
    55921 agatgaaaaa ggtgtgccat tgactagtga atacctaagt gatagttatg gtaaattgaa
    55981 tgcaaaatat tatggtccag cagtcgaaga agacccagaa attaaattcg aatggtcacg
    56041 gataccgcat ttttattata attactatgt tttccaatac tcgactggct tttctgctgc
    56101 ttcagcactg gcgaagaaaa tactaaacca ggaaccagag gcactagaga actacttggc
    56161 ttacctaaaa gcaggtaaca gcgattatcc tgtggaagta atgaaaaaag caggagttga
    56221 tatgacacag gctgcatata ttgaagatgc aatgtcgatg tttgaacaac gtctaaatga
    56281 attagaagaa ttgatcgatc gtttataagg ctttctattt ttgattttct cgaatagaaa
    56341 cgaaaaaaat acgtcggctc tcttgtaagg gagtcgacgt attttttta
//
LOCUS       DACGLW010000007        55830 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.007, whole genome shotgun sequence.
ACCESSION   DACGLW010000007 DACGLW010000000
VERSION     DACGLW010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 55830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55830
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.007"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            <1..178
                     /locus_tag="IXT53_000511"
     CDS             <1..178
                     /locus_tag="IXT53_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAQ4904365.1"
                     /translation="KTVETVFSSLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRA
                     QRRFEGTLRYSLGY"
     gene            complement(313..828)
                     /locus_tag="IXT53_000512"
     CDS             complement(313..828)
                     /locus_tag="IXT53_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-keto-6-deoxy-D-glucose epimerase"
                     /protein_id="HAQ4904366.1"
                     /translation="MVVVMVKVFDIIKFSDFRGYTAVALDNETLFSIGIEFHIKQINQ
                     GFSHNKYTMRGLHYQSGEHAQGKIVMCLHGEIMNIALDIRQGSSTYGCVYVNTLSESN
                     GKCAYIPRGYAHGYLTMEDNTLVQWCVDNDFNSNAAKCISYESVDIEWPVSRQCFVIS
                     EKDKNVERLSF"
     gene            complement(1010..1621)
                     /locus_tag="IXT53_000513"
     CDS             complement(1010..1621)
                     /locus_tag="IXT53_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAQ4904367.1"
                     /translation="MIDIISAITVNVLTALYQPFLFAVLTAYLFMYWYLFIVDNSRDP
                     KNLKDSVLIWLRYFKASSKFRRLFFLAFYFTLILFKTLLNRNMWMNPLSNVIGQWSLY
                     IIDNATGESRLTTECVENTMLFIPFCMLLLWWRDIKCDIIRTIYVGIKYVFLFSLSIE
                     FTQLFFRLGTFQLSDLFYNTLGGLIGALLYWLFYRLNKYIDLK"
     gene            complement(1647..3098)
                     /locus_tag="IXT53_000514"
     CDS             complement(1647..3098)
                     /locus_tag="IXT53_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAQ4904368.1"
                     /translation="MTSNNLIVKTLSRYKNTPLPVKAALWFMVCGFLQRGVSMITTPI
                     FTRVLSTEQYGLYSTYLSWESLLLMITSLSLFKAMMNLYVKYDNQEHILSTVSGLTLV
                     LTIFWFVIAIFIKDILAEILGLTPVLVVCMFSYFIFQAEIQCWSLHKRYIYDYKTLLV
                     ATLAVTVGSSVVGCLAVLFISPTAEARALMNVLVTGVVGVAVYISVFRRCKDFKDNEV
                     WNFAVVFCVSLMPHYLSEFILNGSDKIMINYLCGSSDVAIYSVAYSIGALINLVTNSV
                     NSTFAPYQYQKIKSGEYDLLSKRANEVLGLVAAMLFALMLFSKEIVLIFGGQKYIDSV
                     NLIVPICIGIYFNYMFQLFARVQEYFEKKLTVVIPSVLCAIMNIVLNYVFIKLYGYQA
                     VAYTTVFSYMFFCVIHYIFYRKVCKELLDGKNIYDVRGILLISAITIAGGILISIINK
                     FDFIKYLIIAIACVAAIIFRKRLLLLVRRIMSK"
     gene            complement(3122..3853)
                     /locus_tag="IXT53_000515"
     CDS             complement(3122..3853)
                     /locus_tag="IXT53_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4904369.1"
                     /translation="MEKKMTVIITMAGLGSRFRKIGYAVPKYMINAKGRTLFDWAMDS
                     LVGYNKNVDKYIFVVRQEDNASAFIKEHCAYFGIENVEVIEIDYMTDGQATTCMLAIP
                     YCNQDNEIMVYNIDTYVESNEMKYEDICGDGHIPCFHADGDHWSFAKLNDRNEVIEVR
                     EKVRISDNCTLGAYYFSSAKLYKALYEEYYADSSNMEKNEKYIAPLYNFMIKKGLKVT
                     ISIVDAKKVHVLGTPEELNVFLNQE"
     gene            complement(3871..4266)
                     /locus_tag="IXT53_000516"
     CDS             complement(3871..4266)
                     /locus_tag="IXT53_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HAQ4904370.1"
                     /translation="MYSEYSFVFDIDGTLCPIKKKDERYEDLVPYENMVEKLRFYKEN
                     GAKITLFTSRNVNSYKGNLGMINANTAKVLLAWLDKWDIPYDEILYGKPWPGHKGFYV
                     DDRTVRPDEFLRCSVDELNEICNSSKKRF"
     gene            complement(4368..6005)
                     /locus_tag="IXT53_000517"
     CDS             complement(4368..6005)
                     /locus_tag="IXT53_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HAQ4904371.1"
                     /translation="MKVLIPAAKVVEKELQSIGMLPAIIYPINKKIVFDYLYNQYNAI
                     CDSLDIICYEKADKVHRRLKNYHDSRIHICDLPELGDLGQTVYFGLSDEDEPVLINFA
                     DTIVMESMSDIEMDTFYYSEDFVSSTWTYFKINEGIITDIHDKEEAYVSDKREKLFIG
                     VFMFSSSKLLKSCLREAVESRKQGGINSFYRALQLYSERCPLKAVRTEKWFDIGHIDK
                     YYNTKLEVKAREFNHISIDKDRGILKKTSDDKDKFIGEIKWYLKLPADIEYVRPRIFD
                     YSTSYVAPYVDMEYYAYHTVHELYLYGDLKYNQWHDLFSRIKFICSDFKRYTLKDEKI
                     RESLEDMYLRKTMQRFQKLSEDKRFSAFFEKQIQINGRKFISLTGVIELLKNLIPSML
                     YDVNEFCIIHGDLCFANMMVDSNCSFVKVIDPRGKFGKYDIYGDSRYELAKLFHSLDG
                     KYDYIIKDLFTVEYDTECAVIDYTINTRVRDYDLFELFKSVFSDEIKDDFDKIVLIEA
                     LLFLSMIPLHGESFDHQIVMLATGMELLARVSNKVIL"
     gene            complement(6040..7374)
                     /gene="gmhB"
                     /locus_tag="IXT53_000518"
     CDS             complement(6040..7374)
                     /gene="gmhB"
                     /locus_tag="IXT53_000518"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HAQ4904372.1"
                     /translation="MKTVIMAGGRGSRISELFPDIPKPMIPIDGIPVLERELCSLRDQ
                     GFNDIILTVSYLHEKIEEHFGDGSKLGIKIKYFIEKTPLGNAGALYFLLDELGDEPFL
                     LLNADAVFDVDFRQMIRFHVENNADVTLFTHPNSHPYDSGLIISDDNKKVLQWLAKED
                     QRPEYYKNRVNAGLHVINPKVLRENPLMEIVNKSIVEGGGLPYKIDLDRMILKPLANT
                     GRMYCYDSPEYVKDMGTPDRYRAVIKDFQKGIIVGKNLSNPQKAIFLDRDGTINKYVG
                     FLRNAEEFELIDGVSEAIKIINSLGFLCIVITNQPVIARGEVTVDELNSIHNKLETML
                     GHAGGFLNGIYYCPHHPHKGYAGEVIELKIDCNCRKPKPGLLLEASKDFNINLSQSFM
                     VGDSESDVIAGKNAGCKTVLITEDITLPANYGQDYSVCSLLEFVKNHLCTMQ"
     gene            complement(7416..8525)
                     /locus_tag="IXT53_000519"
     CDS             complement(7416..8525)
                     /locus_tag="IXT53_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4904373.1"
                     /translation="MNKKEILYNILKKYENLPYVFRNMFGHNISKLVSWKLTADCATV
                     EENSEVYAAFQLRSKDMSDVPVMGLVNCCKDVAIVMQGPLCLKDDFSYNTLAYYKKCY
                     PNALIILSTWKDEDIKALKRIEDLGVIVICSEKPEKPGHLNINYQVGNTLPGIVRAKQ
                     LGAKYVCKTRTDQRIYHPNAMAFFCSLLEQYPVNNEDDWKLIQNQRLLLLSMPYGDMF
                     FPYCLSDFLYFGDVDDMINLFSIPSDVREKGAVSRGVSRREISENNLAPEVQLLRSYI
                     SRMGGNEECSIRAYWEFVKNHVITINKNQIDLYWHKYVGRYSNNTIYGTYYIDDSQDA
                     LHCYNFDFINWLNLYTGKYEYKAEYEKYMDFVWEE"
     gene            complement(8550..9785)
                     /locus_tag="IXT53_000520"
     CDS             complement(8550..9785)
                     /locus_tag="IXT53_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4904374.1"
                     /translation="MQISKWKIIQFLMVFLMIISSSILKPLGGWLGETMNVRLVTVIL
                     AIVCLLIASLPAFRKNRYLNFCTLLICVVIIAAYIYSDKTIWVKSRESMSLYYVLLAY
                     PLFCTLINQAWNLDSMLNTICGVTFLSYILRTSISLIQKFSGVLLYENIYRECAPENW
                     YRGGFLRINPPCFTIIIIPIALYMIERQVVARRKVKYYLLIASALAYSAVIHGSRSVL
                     VYQIIVLGCYILFKKGSSKRKIAMWVLAGLLVVIFINSTMFSTFVETFTNSTGEYAGS
                     AESRFASVWFFIPKFLQSPLFGTGILTGEEFTFILPGGVRGHLSDIGFFYTITQYGVG
                     ILIFDILFIIKGFKTALLASKVGMTGYQYLAFGITLNVLLTGINIDWFYPIFTPAIPV
                     CLAVIEYIYQECRSVANIE"
     gene            complement(9816..10946)
                     /locus_tag="IXT53_000521"
     CDS             complement(9816..10946)
                     /locus_tag="IXT53_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ4904375.1"
                     /translation="MIGYKVSIMKVLMCESQMPENWYSFDLSKELNQYVDLTLLCKRD
                     VPPLDPKIKSKHILYHKSTNKVFSALVFLKSLFLIWKEIRFGDYDIVHLQLMKSLKYE
                     GGLYVLCKRHYKKLIMTVHNVLPHEVREKDRQEHFNIYSNCDGFIVHNRVTEQHLYAE
                     FPELNAKSYVCPHAIYGDVKHISRAYDKDKFEFILFGQIRPYKGIDVLLNAITELPMD
                     VRKKVHFTIAGQQHPKQDSTDYKKMIEDLKVSDCVLYKPERISDEEMDSMFRNADAAI
                     FPYKEIYGSGALLMAYTYCLPTIVSNVPAFMEETEEGKIGLLFENLNSKSLADTIVKY
                     VNLSPAEKSKIEDDIYELVNRKYTWAASAKLTYEAYKQILNE"
     gene            complement(10933..11706)
                     /locus_tag="IXT53_000522"
     CDS             complement(10933..11706)
                     /locus_tag="IXT53_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ4904376.1"
                     /translation="MLYFVIPLMSAKAANNWEMVSTLFNRTLWSCYNQTDPDFKIIVA
                     CHEIPKLTHEYDDRVEFIQVSEKDAPIPTNQDEKMIDKGYKTHALAMRLRELGGGFAM
                     MVDADDLVSSHLAEYVNSHPQENGFYVKTGYVYFVGDDYMKVLPKFSSGSACIVKYAV
                     EDLPEAYPEVMTANCDDNPWIIRKRHGGVVPACEEQGRPLKPLPFKAAVYVLGTGDNH
                     SLYGKTTKYQTKMREILEMFIAKRKIKGKLKAEFSVDWL"
     gene            complement(11720..12175)
                     /locus_tag="IXT53_000523"
     CDS             complement(11720..12175)
                     /locus_tag="IXT53_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HAQ4904377.1"
                     /translation="MNAYKLYKVERWFYLHKMEFFAKIVKALIYVLHNSSISYQTEIG
                     DNCKFLYGGIGCVIGKDTKIGNHVIIGTNVLTGGRSNKPGMPTIGNNVYIATGAKILG
                     NITIGDNVIVGANAVVINDVEPNCSVGGVPARVLKKDIDVNDYCSLNRY"
     gene            complement(12374..12607)
                     /locus_tag="IXT53_000524"
     CDS             complement(12374..12607)
                     /locus_tag="IXT53_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4904378.1"
                     /translation="MINDMEVTKLMALRNRYALNIVDNCTRKIAKILGCCIGKGAQIG
                     NSVEFVHNSVGTVIHSDTILEDGVKVYQNVTCG"
     gene            complement(12597..13703)
                     /locus_tag="IXT53_000525"
     CDS             complement(12597..13703)
                     /locus_tag="IXT53_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ4904379.1"
                     /translation="MKILIVVTMTNNFGKKGSYNSQEIGLARGLAELGHEVIVYKSVK
                     SDEEVGTEQLSNTAMIHYIKCRYVGSNGFLSTKNFDKNADVLIQFADLQLSVPHVYSW
                     TVKNGIKYIPYNGITRSQSGNKYIKTIIEILAHRNYKIYKKNGCVAKNDRVSEKLVQL
                     GVNKVKLAPVCVDFEQLHKNYEAIDFTTLREKWNIPRNVRVILYVGRFEKDKRPLDMI
                     SLFNKLDEHNYLLMVGTGALLEQTKQQINLLGLQNRVKIIERIPNKDIWELYCLSDVF
                     VNLNYTEIWGMVLLEAMYYRLGIIAVNAPGPQNIILDNRYGMLAKSDDEILTFLKDGR
                     FTNDIIDAAYQRVTTEFRWSKSGEIMLSMLEGDK"
     gene            complement(13721..14482)
                     /locus_tag="IXT53_000526"
     CDS             complement(13721..14482)
                     /locus_tag="IXT53_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HAQ4904380.1"
                     /translation="MEKNMYKRKRDFTVCGIVGVCATTKNEFVREVFSMPTSPVSATV
                     DLVGVPAIISAKENPKIAEMYSQATMAAIDGMPIVKIGRRKGFTCERCAAPDIMGMVF
                     EEGVRQGKTHYFYGGKDNEVLNKLRANLERDYPGIKIVGMYSPPFRPLNEEEDSKICE
                     EINNLNPDFLWVGIGAPKQEMWMWEHQKKIHNTVMFGVGAGFNFFAGTLEKAPEWMEK
                     ASLEWLFRLVKEPRRLWRRYILGGIKYIYYTIESK"
     gene            complement(14558..15556)
                     /locus_tag="IXT53_000527"
     CDS             complement(14558..15556)
                     /locus_tag="IXT53_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAQ4904381.1"
                     /translation="MKKTIMVTGGCGMIGSNLVKRLVKEGCWDVYVADNLWRGKLEYL
                     NDEDGHPVIDLDTHFFNADLTDYEQAKKVIGTTEYVVHLADVVAGIDYVFKNQGELFR
                     INNLINSNVFDCCRKAGKEKIKGVIYVGTVCSFPLTRQNTLNPEPLREEELFPALPES
                     AYGWSKLMGQLEMGYLEKETGIPCCILMLHNVYGTPTDFGERSQVIPALIRKAINNPE
                     EPFNVWGSGEQGRAFIHVNDIVNALVLALDKGWGHGHIQIGPSHCTSIKEIAYKIIEI
                     SGKDIKPFFDTSKPEGDKARCADYSKAKEILDWEPTVSLEEGLRESYNWIEKQIKG"
     gene            complement(15575..16228)
                     /locus_tag="IXT53_000528"
     CDS             complement(15575..16228)
                     /locus_tag="IXT53_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ4904382.1"
                     /translation="MLAEKLNKHIDLLIKRYPTLETIKQDIIDGYLIMEECYLNGGKL
                     LVAGNGGSAADSEHIAGELMKRFKTPRPVSKEFADKLVAIDPVRGPELAKNLECGLTA
                     IPLVAHEALSTAYINDVDGLGVFAQQLFGFGRSGDVFLGISTSGNSKNIMSATVVARA
                     LGIKVIGLTGAKGGELATVADVCVKAPEVETYMIQEYHLPIYHCWCLMLEDKFFGNE"
     gene            complement(16238..17248)
                     /locus_tag="IXT53_000529"
     CDS             complement(16238..17248)
                     /locus_tag="IXT53_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HAQ4904383.1"
                     /translation="MIITKTPFRMSFFGGGTDMEDYFKENGGAVLSTTFDKYCYVNVR
                     HLPRFFDYSAELSYSRTERVTSIEDIEHPAIRNAMKMLDMHEIRLTYEADLPARSGLG
                     TSSSFAVGMLNAFYALEGKYADKKKLADEAIYLERVLCNEAGGWQDQIAASYGGFNRI
                     NFNVDGYEVLPVIISPERKKQLNNNLMMFFTGFTRFSSDVQKANNVSGTEDKRVRLKK
                     MYELVDEAEAILTDKNRNLDDFGRQLDVTWRLKKGTGGAISTGSIDELYEKGMAAGAL
                     GGKLLGAGGGGFLVFYVQPEKQDAVRWAMRDLMYIPFEFENGGTRVIHYTPETYIEKQ
                     NG"
     gene            complement(17267..18517)
                     /locus_tag="IXT53_000530"
     CDS             complement(17267..18517)
                     /locus_tag="IXT53_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HAQ4904384.1"
                     /translation="MKKQYNIAVAGTGYVGLSIATLLAQHHKVTAVDIIPEKVELINN
                     RKSPIQDDYIEKYLAEKELNLTATLDAKAAYQSADFVVIAAPTNYNSRTQHFDTSAVE
                     AVIRLVMEVNPDAIMVIKSTIPVGYTASVRVKTGSKNIIFSPEFLRESKALYDNLYPS
                     RIIVGTDMDDERLVEDAHTFATLLQEGAIKENVDTLFMGFTEAEAVKLFANTYLALRV
                     SYFNELDTYAEMKGLNTQQIINGVCLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYA
                     DVPENLIEAIVESNRTRKDFIADRVLQMAGYYNYAEANQYDAGKEKPVVIGVYRLTMK
                     SNSDNFRQSSIQGVMKRVKAKGATVVIYEPTLKDGETFFGSKVVNDLEQFKAMCDSVI
                     ANRYDACLDDIKEKVYTRDIFQRD"
     gene            complement(19863..20036)
                     /locus_tag="IXT53_000531"
     CDS             complement(19863..20036)
                     /locus_tag="IXT53_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1972 domain-containing protein"
                     /protein_id="HAQ4904385.1"
                     /translation="MNRYVHGTVQKKNRKINGKLLVNPNGYEWLRAKGSYPVPKYCNY
                     SETLMEWYQLIIT"
     gene            complement(20379..21068)
                     /locus_tag="IXT53_000532"
     CDS             complement(20379..21068)
                     /locus_tag="IXT53_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAQ4904386.1"
                     /translation="MESEDLLSRISEVHPNQVWLDKEKVEKRYIYLFFKRMIDIVGSI
                     IGLILASPLMFYVAYRIRKEEPGSPIFFCHTRVGKNGKTFKMYKFRSMCLDAEEKLEE
                     LLDQNEIEGAMFKMKEDPRVTKIGKIIRAKSIDELPQLWNVLKGDMSLIGPRPPLERE
                     VAEYTRYDLQRLYVKPGCSGLWQVSDRNNVSFHKMVELDLEYISHRSLMYDLRLILKT
                     FYVIVKPNGAY"
     gene            complement(21113..21877)
                     /locus_tag="IXT53_000533"
     CDS             complement(21113..21877)
                     /locus_tag="IXT53_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HAQ4904387.1"
                     /translation="MIDLHCHILPGIDDGAENIDASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIDRDEILFTDLD
                     DTYLLIEFPTLEVPLYAEQLFFELLQLGKTPVIVHPERNAHFRKDPNHMLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKMMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKDFG
                     KEKVVQMKQVAKDLINGDEISYPVYEEVKKKKFGLF"
     gene            complement(21905..22603)
                     /locus_tag="IXT53_000534"
     CDS             complement(21905..22603)
                     /locus_tag="IXT53_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HAQ4904388.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVENLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENMSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            complement(22616..23395)
                     /locus_tag="IXT53_000535"
     CDS             complement(22616..23395)
                     /locus_tag="IXT53_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HAQ4904389.1"
                     /translation="MEETISLKEIFETLRKHVITILISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     GQIKDAISVNQSQNSQMFSIQATSTNAVTAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLVIGLVLGMMVGVGLAFLFELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAPTQPKATNDKTAPSRRSRSRV"
     gene            complement(23411..24355)
                     /locus_tag="IXT53_000536"
     CDS             complement(23411..24355)
                     /locus_tag="IXT53_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ4904390.1"
                     /translation="MAKRRRRSRGKKIFLIILAILVVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGHDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFTIGKITLNGDQALSYSRMRYEDPNGDYGRQER
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMSFDEMKKIALDYRDAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            complement(24396..25175)
                     /locus_tag="IXT53_000537"
     CDS             complement(24396..25175)
                     /locus_tag="IXT53_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4904391.1"
                     /translation="MEKPFVKLLATVAVGTGAIVICLFGYHFNNQRQHHQRINYAESA
                     ITNQKDTVTSLSKEVDKLYSTKEKIFLNPEITEETVSNLSHKLSSIKLSADDYDIKES
                     ELPKEAAAIQEEKKAVLTQLEDAESKLKIQTAVNDLFTKNVSNWQQAVDDVVIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADTQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTALSLIEQIRNEKIRAAYASKAETVAQQMGYSNTSY"
     gene            complement(25560..27635)
                     /locus_tag="IXT53_000538"
     CDS             complement(25560..27635)
                     /locus_tag="IXT53_000538"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HAQ4904392.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKESKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIDAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAQLEKQSEVDPELFENDAEKELHKAVEA
                     MKASFADQTIHENYDQLVALRPLIENYFDQTMVMADEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(27637..28554)
                     /gene="glyQ"
                     /locus_tag="IXT53_000539"
     CDS             complement(27637..28554)
                     /gene="glyQ"
                     /locus_tag="IXT53_000539"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HAQ4904393.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENVKEAE"
     gene            complement(28940..29752)
                     /gene="recO"
                     /locus_tag="IXT53_000540"
     CDS             complement(28940..29752)
                     /gene="recO"
                     /locus_tag="IXT53_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HAQ4904394.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRHIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQLMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKYGGVICERHWSMDDHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRELIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTSEKDMSEKDLPKKEE"
     gene            complement(29895..30794)
                     /locus_tag="IXT53_000541"
     CDS             complement(29895..30794)
                     /locus_tag="IXT53_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HAQ4904395.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(30809..31210)
                     /locus_tag="IXT53_000542"
     CDS             complement(30809..31210)
                     /locus_tag="IXT53_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HAQ4904396.1"
                     /translation="MPMDLKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFG
                     ILALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKD
                     MAAGAVLVTACFSVIVGLILFVPKIYHLLFN"
     gene            complement(31188..31664)
                     /gene="ybeY"
                     /locus_tag="IXT53_000543"
     CDS             complement(31188..31664)
                     /gene="ybeY"
                     /locus_tag="IXT53_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HAQ4904397.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLELPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISTER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(31681..33873)
                     /locus_tag="IXT53_000544"
     CDS             complement(31681..33873)
                     /locus_tag="IXT53_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HAQ4904398.1"
                     /translation="MLNKTLQKLQKKLGKAALPFLLFLFFIIVCGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNDKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSVV
                     LMKFFQLFQTEQAAYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTVILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIIYQGMSFGD
                     ATTWLTLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICRQHHGTTLMRYFYVKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKSEVEKIKEEQEE"
     gene            complement(33897..34868)
                     /locus_tag="IXT53_000545"
     CDS             complement(33897..34868)
                     /locus_tag="IXT53_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HAQ4904399.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            35574..36953
                     /gene="brnQ"
                     /locus_tag="IXT53_000546"
     CDS             35574..36953
                     /gene="brnQ"
                     /locus_tag="IXT53_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAQ4904400.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            complement(37109..37555)
                     /locus_tag="IXT53_000547"
     CDS             complement(37109..37555)
                     /locus_tag="IXT53_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HAQ4904401.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(37582..37758)
                     /locus_tag="IXT53_000548"
     CDS             complement(37582..37758)
                     /locus_tag="IXT53_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HAQ4904402.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            complement(37920..38348)
                     /locus_tag="IXT53_000549"
     CDS             complement(37920..38348)
                     /locus_tag="IXT53_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ4904403.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            38440..39300
                     /locus_tag="IXT53_000550"
     CDS             38440..39300
                     /locus_tag="IXT53_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HAQ4904404.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLMQALQDALKEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            complement(39394..39687)
                     /locus_tag="IXT53_000551"
     CDS             complement(39394..39687)
                     /locus_tag="IXT53_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAQ4904405.1"
                     /translation="MPDTQKTKVEKVSQLFKMLSDPTRLKILLYLKDGEQNVTAITQA
                     VEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHVLDILNQTLKHVEHQ"
     gene            complement(39760..41487)
                     /locus_tag="IXT53_000552"
     CDS             complement(39760..41487)
                     /locus_tag="IXT53_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAQ4904406.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLIDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDDEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAMRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     AIIKKMTEE"
     gene            complement(41701..42627)
                     /gene="trxB"
                     /locus_tag="IXT53_000553"
     CDS             complement(41701..42627)
                     /gene="trxB"
                     /locus_tag="IXT53_000553"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HAQ4904407.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            complement(42772..44205)
                     /gene="ascB"
                     /locus_tag="IXT53_000554"
     CDS             complement(42772..44205)
                     /gene="ascB"
                     /locus_tag="IXT53_000554"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ4904408.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFPV
                     ITGKKKMFEFDKDHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     NEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFASV
                     KNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDTVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            complement(44205..45608)
                     /locus_tag="IXT53_000555"
     CDS             complement(44205..45608)
                     /locus_tag="IXT53_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HAQ4904409.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMATMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     DLIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            45838..46740
                     /locus_tag="IXT53_000556"
     CDS             45838..46740
                     /locus_tag="IXT53_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ4904410.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            complement(46781..48667)
                     /locus_tag="IXT53_000557"
     CDS             complement(46781..48667)
                     /locus_tag="IXT53_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ4904411.1"
                     /translation="MGKYQELARKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAAGGMIRGLTAFLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEAGETDDRQKRSGKEIVKTPIQGQIAPLKAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSETGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEKEGYSLETPVIVTNYMDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(48764..49615)
                     /locus_tag="IXT53_000558"
     CDS             complement(48764..49615)
                     /locus_tag="IXT53_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ4904412.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQNNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            complement(49950..51734)
                     /locus_tag="IXT53_000559"
     CDS             complement(49950..51734)
                     /locus_tag="IXT53_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4904413.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGVYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFVAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            complement(51734..53485)
                     /locus_tag="IXT53_000560"
     CDS             complement(51734..53485)
                     /locus_tag="IXT53_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ4904414.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQQLEAKIEGGEA"
     gene            complement(53627..53851)
                     /locus_tag="IXT53_000561"
     CDS             complement(53627..53851)
                     /locus_tag="IXT53_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HAQ4904415.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            54102..55688
                     /locus_tag="IXT53_000562"
     CDS             54102..55688
                     /locus_tag="IXT53_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HAQ4904416.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFVVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADQIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQETIEEIE"
BASE COUNT        16305 a        12026 c         9178 g        18321 t
ORIGIN      
        1 aaaaacagtt gagactgttt tttcttcctt agaaaaatta ggttgtcaaa atttcaactc
       61 acgttctgtt aaagggctag agagtagatt tgaaagcata ttactggctt acagtgttct
      121 attaagtcga gcacaacgac gttttgaagg aactttgaga tattctttag gatattaaaa
      181 ttaactagca ccacgggtat atctatatct aaatttagtt ttatgtattc ctcatagaaa
      241 taaccgagca tttgctcggt tattgttctt cttattttcc agtatccttc ttttctccac
      301 agctcatttt aatcaaaagc ttaatctttc cacatttttg tctttctcac ttataacgaa
      361 acactgccga ctgaccggcc attcaatatc tacgctttca taacttatac atttggcagc
      421 attagaattg aaatcattat ctacgcacca ctgaactagc gtattgtcct ccatagtaag
      481 atagccatga gcatatccac gtgggatata cgcacattta ccattgcttt cactcaatgt
      541 gttgacatac acacatccat aagtggaaga tccttgtcga atatcaagtg cgatattcat
      601 aatttcacca tgtaggcaca tcactatctt tccttgagca tgctctccac tctgataatg
      661 tagccctcgc atagtatatt tattatgact aaatccttga tttatttgct taatgtgaaa
      721 ctcaatacca atcgagaaaa gagtctcatt atccaatgca acagcagtat atcctctgaa
      781 atccgaaaac ttaattatat caaacacttt taccataact accaccatgt tatatatttt
      841 accaattttt taattcgctc gacctcattt aaagcaataa aattccctac ctcttgaaga
      901 tataacaaca atgttttagc aaaaatagat ggcatgtaaa gtattcttga tgagattaca
      961 caatcctgtc tgcagaaaaa tattaaccat attaaaattc aaagcagttc tattttaagt
     1021 ctatatactt atttaatcgg tagaatagcc aatatagcaa agcaccaatt aagcccccta
     1081 gggtgttata aaacaaatcc gatagttgaa atgtaccaag cctaaaaaac agttgtgtaa
     1141 attctatact taatgagaat aaaaacacat acttaatccc aacatagatg gtgcgtataa
     1201 tatcacattt aatgtcacgc caccataaca ataacatgca aaaagggata aacagcatcg
     1261 tgttctccac gcattctgtt gtcaatctgc tctctcctgt agcattatct ataatatata
     1321 atgaccactg tccaataacg tttgaaagcg ggttcatcca catatttcga ttaagcaagg
     1381 ttttaaataa tatcaaagta aaataaaacg caagaaaaaa gagtctgcga aatttactag
     1441 atgctttaaa atatctcaac cagattaata cagaatcttt taagttttta ggatctcgag
     1501 agttatcaac tataaataga taccagtaca taaacagata cgctgttaat acggcaaata
     1561 agaacggctg atataatgct gttaatacat ttaccgttat agcagaaata atatctatca
     1621 ttctctatct ctctatgagc aaatgtttat ttgctcataa ttctccttac taaaagtagc
     1681 aatcgttttc taaatataat tgcagcgaca caagcaattg ctataattaa atattttatg
     1741 aaatcaaatt tattaattat tgaaattagg atgccaccag ctatcgtgat tgcagaaatc
     1801 aaaagtattc ctctgacatc atatatgttt tttccatcca atagttcttt gcaaactttt
     1861 ctataaaaaa tataatggat gacgcagaaa aacatatatg agaatacagt tgtgtatgct
     1921 accgcctgat acccatataa ctttataaaa acataattta gtacaatatt cataattgca
     1981 cacaaaaccg atggaataac gacagtgagt ttcttttcaa agtattcctg tactctagca
     2041 aacagctgaa acatatagtt gaaataaata ccgatacata taggtacaat taaattaaca
     2101 ctgtcgatat atttctgccc accaaatatc aacacaattt ctttactaaa tagcattaat
     2161 gcaaaaagca tagccgctac caaaccaaga acttcattag ctctttttga tagtaaatca
     2221 tattctcctg acttgatttt ttgatactga taaggtgcaa aagtggaatt gacagaattt
     2281 gtaacaaggt taattagagc accaatagaa tatgccacgc tgtaaatagc aacatctgac
     2341 gaaccacata agtaatttat cataatttta tcagaaccat tcagaataaa ttcagataaa
     2401 tagtgtggca tcaacgatac acaaaatact actgcgaaat tccacacttc attatcctta
     2461 aaatccttac atcttctaaa taccgatata tacacagcca cgccaacaac accagtcact
     2521 aggacattca ttaaggctct cgcctcagcg gtaggagata taaacagaac cgctaaacag
     2581 ccaactacag atgagccaac agtaactgca agggtagcta ctaataaagt cttataatca
     2641 tatatatatc gtttgtgtaa agaccaacat tgtatttctg cctgaaatat aaaatatgag
     2701 aacatacaga caactagaac tggtgtcaat cctaaaattt ctgccaatat atccttaata
     2761 aaaatagcaa taacaaacca aaaaatagtc agtactaaag tcaatccgga tactgtcgaa
     2821 agaatatgtt cttgattatc atattttaca taaagattca tcatggcttt gaacaaacta
     2881 agcgatgtaa tcatgagcaa taaggattcc cacgatagat aagtgctgta taaaccgtat
     2941 tgttctgtag acaaaactct agtaaaaatt ggggttgtaa tcattgaaac ccctctctgt
     3001 aaaaaaccac ataccataaa ccataatgca gccttaacag gaagtggagt attcttatat
     3061 ctgcttaatg tttttacaat tagattatta ctggtcactg ctattttctc ctaaacacta
     3121 tttattcttg attcaaaaac acattgagct cttctggagt accaagcaca tgaacctttt
     3181 tcgcatctac tatgctaata gtcaccttta accctttttt tatcataaag ttatataatg
     3241 gtgctatata tttttcattc ttctccatat tagaggagtc tgcataatac tcttcatata
     3301 aagctttata cagctttgcc gatgagaaat aatacgcacc aagagtacaa ttatcagaaa
     3361 tgcgaacttt ttcgcggact tctattactt catttcgatc gttaagcttt gcaaaactcc
     3421 aatgatcgcc atcagcatga aagcatggaa tatgtccatc tccacaaata tcttcgtatt
     3481 tcatctcatt tgattccacg tatgtatcaa tattgtaaac cataatctca ttgtcttgat
     3541 tgcaataagg aatagccaac atacatgtag ttgcttgacc atctgtcata tagtctattt
     3601 caatcacttc tacgttttca ataccaaagt aagcacaatg ctcctttata aaagctgaag
     3661 cgttgtcttc ctgacgaaca acaaaaatat acttatcaac atttttattg tatccaacca
     3721 aactatccat ggcccaatca aataaagttc ttcctttggc attaatcata tatttaggaa
     3781 ccgcataacc aatttttcga aaacgacttc ccaaacctgc catcgtaatg ataactgtca
     3841 tctttttttc catagaacta acctccttat ttagaatctt ttttttgaac tgttgcagat
     3901 ttcatttaat tcatctacag agcatctcaa aaactcatca ggtctaaccg ttcgatcatc
     3961 tacatagaag cctttatgcc ccggccaagg cttaccatac aggatttcat catacggaat
     4021 gtcccattta tccaaccacg ccaagagcac ttttgcagta ttagcattta tcattcccaa
     4081 atttccttta taggaattaa cattgcgtga ggtgaacaat gtaattttag caccattctc
     4141 cttatagaat ctcagttttt caaccatatt ctcatacggc accaaatcct catatcgctc
     4201 atccttcttc tttatcggac acaaagtgcc atcgatatca aacacaaaag aatattcact
     4261 atacattttt ctcttccctt ctattgtatt aattaatata atcgtgcctt aatgcctaca
     4321 ttgctaaaac agtattgtaa ccgttattca ttaaggcaca atttatatta gagtataact
     4381 ttattactta ctcttgcaag aagttccata cctgtagcaa gcatcactat ttggtgatca
     4441 aaactttcac cgtgtaatgg aatcatacta agaaatagca acgcttctat caatacaatc
     4501 ttatcaaaat cgtccttaat ctcgtccgaa aagaccgact taaacaattc aaataaatcg
     4561 taatctctta cacgcgtatt aattgtataa tctatcactg cacactcggt atcatattct
     4621 acagtaaata gatccttgat gatataatca tatttaccat ctaaactatg aaatagtttt
     4681 gctaattcat atctgctgtc cccataaata tcatattttc caaattttcc cctaggatca
     4741 ataaccttaa caaaggaaca attggaatct accatcatat tagcaaaaca caaatctcca
     4801 tgtataatac aaaattcatt tacatcatag agcatacttg ggataagatt ctttagaagt
     4861 tcaattacac cggttaaaga aataaactta cgaccattaa tttgaatttg tttctcaaaa
     4921 aaagctgaaa atcttttatc ttcagacaat ttttgaaatc tctgcatggt ttttcgcaaa
     4981 tacatatctt ccaatgactc tctaatcttt tcatctttta aggtgtatcg cttaaaatcg
     5041 ctacagataa actttatacg gctaaacaga tcatgccact ggttatattt taaatcacca
     5101 tataagtata actcatgaac cgtatgatat gcataatatt ccatatcaac atagggagct
     5161 acgtaagatg tagaataatc aaaaattcta ggtcttacat attcaatatc agcaggaagt
     5221 tttaaatacc actttatttc tccaataaat ttatccttgt catcgctagt tttcttcaat
     5281 atccctcgat ctttatctat agagatatga ttaaactctc gtgctttaac ttccaacttt
     5341 gtattgtaat atttatcgat gtgtccaata tcaaaccact tttctgttct tacggccttt
     5401 agcggacaac gttcgctgta tagctgtaaa gcccgataaa atgagtttat tcccccctgt
     5461 ttccgactct caactgcctc tcttaagcaa ctcttcagaa gttttgacga agaaaacata
     5521 aatacgccaa taaataattt ttcacgttta tcggatacgt aagcttcctc tttatcgtgg
     5581 atatcagtaa ttattccttc attaatttta aagtatgtcc aagtactcga cacaaaatct
     5641 tcagaataat agaatgtatc catttctata tcagacatac tctccataac aattgtatct
     5701 gcgaagttaa ttagaacagg ctcatcttca tctgataacc caaaatacac ggtttgccct
     5761 agatccccta attcaggaag gtcacatata tgaattctgg aatcatgata gtttttcagt
     5821 cttctatgaa ccttatccgc tttttcataa cagataatat ccaatgaatc gcagattgca
     5881 ttatactgat tatacaaata atcaaagaca atttttttat taattggata gataattgct
     5941 ggtaacattc ctatactctg caattctttt tcaacaactt ttgctgctgg aattaatacc
     6001 ttcatctaat tactcctttt acaagtttca ctaaaccaat tattgcatag tgcataaatg
     6061 atttttaaca aattctaaca atgaacagac actatagtct tggccataat tagcaggcaa
     6121 ggtgatgtct tctgtaatca gtactgtctt acaaccagca ttttttccag caattacatc
     6181 actttcacta tctccaacca taaatgattg acttaggttt atgttaaaat cctttgatgc
     6241 ttctaataac agtccaggtt ttggcttgcg acaattgcaa tcaattttta attctataac
     6301 ctctcctgca tatcctttat ggggatggtg cggacagtaa taaataccgt ttaaaaatcc
     6361 accagcatgc cctagcattg tttcaagctt attgtgaata ctattaagct catcaacagt
     6421 aacttcacct cttgcaatta caggctgatt tgttataaca atacacagaa aacctagaga
     6481 attaataatt ttaattgcct ccgatactcc atcaattaac tcaaattctt ctgcatttct
     6541 caaaaaacca acgtatttat taatcgttcc atctctatct aaaaagattg ctttttgtgg
     6601 attggataga tttttaccaa caataattcc cttttgaaag tccttaatta cagctctata
     6661 tcggtccggt gttcccatat ctttaacata ctctggagaa tcgtagcaat acatacgccc
     6721 tgtgttagcc aatggtttca gaatcattct atctaaatca attttgtatg gtagcccccc
     6781 accttcaact attgatttat ttacaatttc cattaatgga ttttctctca acaccttcgg
     6841 atttataaca tgaagacctg catttactcg gtttttataa tattcaggtc tttgatcttc
     6901 cttcgctaac cattgaagca ctttcttatt gtcatcggat ataattaatc cactatcata
     6961 cggatgtgag ttaggatgcg taaacaaagt aacatctgcg ttattctcaa catgaaatct
     7021 aatcatctgc ctgaaatcaa catcaaatac tgcatcagca ttcaaaagta aaaacggttc
     7081 atcacccagt tcatccaaca aaaaatacaa tgcacctgca ttgccaagtg gagttttttc
     7141 aataaaatat ttgattttaa ttcctaattt tgaaccatct ccgaaatgct cctctatttt
     7201 ttcatgcaaa taggaaacag tcaaaataat atcattaaag ccttgatctc taagcgaaca
     7261 caattctcgt tctaaaactg gaatgccatc tataggaatc attggcttag gaatatccgg
     7321 aaacaattcc gaaattcggc ttcctcgtcc gcccgccata attactgttt tcataatatg
     7381 cccttcccta aagtactttt tactataaaa gttaattact cttcccaaac aaagtccata
     7441 tatttttcat attctgcttt gtattcgtat tttccagtgt acaaattcaa ccaatttata
     7501 aaatcaaaat tatagcagtg aagtgcatct tggctatcat caatataata cgtaccataa
     7561 atagtattat ttgaataacg acctacatat ttatgccagt acaaatcaat ttgatttttg
     7621 ttaatagtaa taacatggtt ttttacaaac tcccaatatg ccctaataga gcactcctca
     7681 ttgcccccca ttcgtgaaat atagcttcga agaagctgaa cttctggagc taaattattt
     7741 tcagatatct cccttcttga gactcctcta gacacagctc ccttttctct cacatcacta
     7801 ggaatcgaga agaggtttat catatcatca acatcaccaa aatacaggaa gtctgacaaa
     7861 caatacggga aaaacatgtc tccatacggc attgatagta acagtaatcg ctgattttgt
     7921 attaatttcc aatcatcttc gttattaaca ggatactgtt caagtaaact gcagaaaaac
     7981 gccattgcat ttggatggta tattctttga tctgttctag ttttacaaac atactttgca
     8041 ccaagctgct ttgctctgac tattccaggc aaagtgtttc caacttgata attaatattt
     8101 aagtgtccag gcttttcagg tttctcacta caaataacta taactccaag atcctctatc
     8161 cttttcaatg ccttaatatc ttcgtccttc catgtcgaaa gaataattaa agcatttgga
     8221 taacatttct tatagtaggc cagagtatta taagagaaat catcctttaa acatagtggc
     8281 ccctgcatta caattgctac atctttgcaa caattaacta aacccatcac tggaacatca
     8341 gacatgtcct ttgagcgcaa ttgaaacgct gcataaactt cagaattttc ttctactgtt
     8401 gcacaatcag cagtcaattt ccaagatacg agtttactta tattatgacc aaacatgttc
     8461 ctaaacacat atggtaaatt ttcgtacttc ttcaatatgt tatataaaat ctctttctta
     8521 ttcatgagat gcactcctat taatatttcc tattctatgt tagccacact tctacattct
     8581 tgatagatgt attctataac cgctaagcat acaggaatag ctggagtaaa tatcggataa
     8641 aaccagtcta tattaatccc tgttagcaag acatttaatg taattccaaa tgctaaatat
     8701 tgatagccag tcatacccac ttttgatgca agtaatgctg ttttgaaccc ttttataata
     8761 aacagaatat caaatataag tatacctaca ccatattgag taattgtata gaaaaatcca
     8821 atatcagata ggtgtcctct aactcctcct ggtaatataa acgtaaattc ctcaccagta
     8881 agaataccag taccgaacaa aggcgattgc aaaaatttag gaataaaaaa ccaaactgaa
     8941 gcaaatcgag attcagcaga tcctgcatat tcacctgtac tgtttgtgaa agtttcaaca
     9001 aatgttgaga acatggtcga attaataaat atgactacaa gcaacccagc taaaacccac
     9061 attgcaattt ttcttttcga agaacctttc ttaaaaagga tatagcaacc aagtacaata
     9121 atttgataga caaggacaga tctagaacca tgaataacag cagaatatgc cagtgcgcta
     9181 gcaatcagta aataatattt tacttttctt ctagctacaa cttgtcgctc aatcatatac
     9241 aaagcaatcg gtattattat aattgtaaaa catggtggat taattcttaa aaagcctcca
     9301 cgataccaat tttccggggc acactcacga tatatgtttt catagagaag tactcctgaa
     9361 aatttctgaa taagtgaaat agatgttcta agaatgtatg aaagaaatgt tacaccacat
     9421 atcgtattta gcattgaatc caaattccac gcttggttaa ttaaagtaca gaacaatgga
     9481 tacgccaata atacatagta taaactcata ctttccctag atttaaccca tattgtctta
     9541 tctgagtaga tgtaagctgc tattataaca acacaaatca gtaaagtgca gaaatttaaa
     9601 tatctatttt ttctaaatgc tggtaatgag gctattaaca aacatactat tgctagaatg
     9661 actgtcacaa gtctaacgtt catcgtttct cccaaccagc cgcccaaagg cttcaatatg
     9721 cttgatgaga taatcattag aaagaccatt agaaactgta tgattttcca tttactaatc
     9781 tgcatgttaa ttattattcc tttctaattg ttttactact cattcaaaat ttgtttgtat
     9841 gcctcgtatg tgagttttgc agatgccgcc caggtatact tgcggtttac aagctcatat
     9901 atatcatcct ctattttgct tttctcagct gggctaaggt tcacatactt tacaattgtg
     9961 tctgctaggc tcttagaatt caaattttcg aaaagtaacc ctatctttcc ttcctcggtt
    10021 tcttccataa aagcaggtac attactaaca atagttggta agcaataagt gtatgccata
    10081 agaagcgctc cacttccata gatttcttta tatggaaata ttgcggcatc tgcatttctg
    10141 aacatactgt ccatttcttc atcagaaatt ctctctggct tatatagaac acaatctgaa
    10201 acctttaaat cttcaatcat ttttttatag tcagtactat cctgtttagg atgttgttga
    10261 cctgctatgg taaaatgaac tttctttctt acatccattg gaagttctgt gattgcatta
    10321 agcagtacat caattccctt atatggacga atttgtccaa acaagataaa ttcaaactta
    10381 tctttatcat acgctctaga tatatgttta acatctccat agatagcatg tggacaaaca
    10441 tatgactttg catttaattc tgggaattca gcatataaat gctgttctgt tactcgattg
    10501 tgaacaatga aaccatcaca atttgaatat atattaaagt gctcttggcg atccttttca
    10561 cgaacctcat gtggtaacac attatgaact gtcataatta attttttata gtgtctctta
    10621 cataaaacgt acaatccgcc ttcatatttg agacttttca tcaattgcaa atgaacaata
    10681 tcataatcac caaatcttat ttccttccaa attaagaaca agcttttcag aaaaacaagt
    10741 gccgaaaaca ctttatttgt acttttatga tacaaaatat gtttgctctt aattttagga
    10801 tctaaaggag gaacatcacg cttacacagc aatgttaaat caacatattg attaagttct
    10861 ttagaaagat caaacgaata ccaattctct ggcatttggc tttcacacat caataccttc
    10921 ataatactca ccttataacc aatcaacaga aaattctgct ttcagttttc ctttaatttt
    10981 acgttttgct ataaacattt ccagtatctc tcgcattttc gtctgatatt ttgtcgtttt
    11041 accataaagc gagtgattgt cccctgttcc taatacatat actgctgctt tgaatggaag
    11101 aggttttaat ggacgccctt gctcttcaca tgctggaaca acgcccccat gtcgttttct
    11161 aatgatccat ggattatcat cacaatttgc tgtcataact tccggatatg cctcaggcag
    11221 gtcttccact gcatatttta ctatacacgc acttcctgat gagaactttg gtagaacctt
    11281 catataatcg tctccaacaa agtacacgta tcccgttttg acataaaatc cattttcctg
    11341 gggatgggaa ttcacatatt ccgccaaatg agaacttaca agatcatctg cgtcaaccat
    11401 catagcgaaa ccgccgccca actctcgcaa acgcattgcg agagcatgtg ttttataacc
    11461 cttatcaatc attttttcgt cttgatttgt gggaataggg gcatcctttt cgctaacttg
    11521 aataaattca actctgtcat catattcatg cgtgagttta ggaatttcat ggcaagcaac
    11581 aatgattttg aaatcaggat cagtctgatt ataacacgac cataatgttc ggttaaataa
    11641 ggtactaacc atctcccaat tgtttgctgc cttcgcactc attaaaggaa taacaaaata
    11701 caacatacaa tctctccttt tagtaacgat tcaaactaca ataatcattt acatcaatat
    11761 cttttttcaa tactctagcc ggaacgcctc ctaccgaaca attaggttca acatcattaa
    11821 tgaccacagc attagcccct actattacat tgtcccctat tgtgatatta cctaaaattt
    11881 ttgcgccagt agcgatatat acattgtttc caattgtagg cataccaggc ttattggatc
    11941 taccaccagt cagtacattc gttccgatta taacgtgatt acctattttt gtatcttttc
    12001 caattacaca acctatgcca ccgtataaga acttacaatt atctccaatt tctgtttgat
    12061 atgaaattga gctattatga agtacataaa ttagtgcctt tacaatcttg gcaaaaaact
    12121 ccattttgtg taagtaaaac catcgttcta ctttgtataa tttatatgca ttcatgtttt
    12181 atctccccat ctttttagca ggtacccccc acacttcgta gggtggaata gacctcaata
    12241 ccactgtatt tgcagcaact acagcacctt ccccaacgca taattctgtg ttagatttac
    12301 aaagaatttt agcacccaca cataaaacag cgttcttttt tactcgaatg atgatacgtt
    12361 cattactatc tgctcaacca cacgtcacat tctgatagac tttaacacca tcttcaagga
    12421 ttgtgtcaga atgaatcaca gtgcctacag aattatgaac aaattcaaca gagttcccta
    12481 tctgtgcacc tttaccaata caacaaccaa gaattttggc tatttttctc gtgcaattat
    12541 caactatgtt taaagcatat cgattcctca atgccattaa tttcgttact tccatatcat
    12601 ttatcacctt ccaacatgct aagcataatt tctcccgact ttgaccatct aaattctgta
    12661 gttacacgct gatatgctgc atctatgata tcgttggtaa atcgtccgtc ttttaagaat
    12721 gtcaaaattt catcgtcaga tttagccaac attccatatc tattatccaa aataatattc
    12781 tgtgggccag gtgcattaac agcaataatc ccgaggcgat agtacattgc ctctagcaaa
    12841 accatacccc aaatttcagt atagtttaga tttacaaata catccgataa gcaatataac
    12901 tcccaaatat ctttgttagg aatacgctca attattttta ctctattctg cagtccaagt
    12961 aaatttatct gctgcttcgt ttgctcgagt aaagcaccag ttcctaccat cagcaaataa
    13021 ttatgctcat caagtttatt aaataaacta atcatatcta acggtctttt atccttttca
    13081 aaccgtccta catataaaat aacacgaaca ttgcgaggaa tattccattt ttctctgaga
    13141 gtagtaaaat ctattgcttc gtaattctta tgtaactgtt caaaatccac acaaacagga
    13201 gcaagtttca ctttatttac tcctaattgt actaattttt cagaaactcg atcatttttg
    13261 gcaacacacc catttttctt ataaatttta taatttcgat gcgccaatat ttcaataatt
    13321 gtctttatat atttattgcc actttgactt cgcgttatcc cattataagg tatatatttt
    13381 attccatttt ttacagtcca tgagtataca tgaggtactg atagttgaag gtccgcaaat
    13441 tgtattagaa catccgcatt cttatcaaaa ttctttgttg ataagaatcc atttgatcct
    13501 acataacgac acttgatata gtgaatcatc gccgtattgc ttaattgctc tgttccaact
    13561 tcctcatcac tttttacact tttatataca ataacttcgt ggcctaattc agctaaccct
    13621 cgagctaatc ctatttcctg agaattatag gaccccttct taccaaagtt attcgtcatt
    13681 gtaactacta taagtatttt catctatttt tattgccccc ctacttagat tcaatagtat
    13741 agtagatata ctttatacct cccaagatat accgcctcca tagtctacga ggttccttta
    13801 ccagtctaaa taaccactct agactagctt tttccatcca ttcaggagct ttttccaaag
    13861 tgcccgcaaa aaagttaaag ccggcgccta caccgaacat tactgtgtta tggatcttct
    13921 tctggtgttc ccacatccac atttcttgct taggcgcacc aataccaacc cacagaaaat
    13981 ctggatttaa gttgttgatt tcctcgcaaa tcttgctatc ctcttcttca tttaacggac
    14041 gaaatggtgg ggaatacatt ccaacaattt tgattcccgg gtaatctctt tctaggtttg
    14101 cacgaagctt atttaacact tcgttatctt ttccaccata gaaataatgt gtttttccct
    14161 gtctaacgcc ttcttcaaaa accattccca taatatctgg tgcagcacat cgttcacagg
    14221 taaatccttt gcgccgtcca attttaacaa taggcatacc atctattgca gccattgtag
    14281 cctggctata catttcagca attttgggat tttcctttgc cgaaataatg gccggcactc
    14341 ccacgagatc aacagtcgca cttaccggag atgttggcat ggaaaaaact tcccttacaa
    14401 actcgttctt tgtagtagca caaacaccta ctattccaca cacagtgaaa tcacgttttc
    14461 ttttatacat attcttttcc atctcagtac atccttactc atatgttctt cctccgcatc
    14521 ctctaattac acggaggaag aacagcttat atacttatta acccttaatc tgcttttcaa
    14581 tccaattgta ggattcacga agaccctctt ccaaagaaac agtaggctcc caatccaaaa
    14641 tctctttcgc cttagaataa tctgcgcaac gagccttatc accttcaggc ttagaagtat
    14701 caaagaaagg cttgatgtct ttgccagaaa tttcaatgat cttatatgca atttccttga
    14761 tggaagtaca gtgagaaggg ccaatctgaa tatgaccatg accccaaccc ttatccaggg
    14821 cgagcaccaa tgcatttacg atatcattga catgaatgaa agcacgtccc tgctctccgc
    14881 taccccacac attaaaaggc tcttcaggat tattgattgc tttacgaatc agagcaggaa
    14941 taacctgact acgttcgcca aaatcagtag gtgtgccata aacattatgg agcatcagga
    15001 tacagcacgg aattccggtt tctttttcca aataccccat ctccaactgg cccatcagtt
    15061 tgctccagcc ataagcagac tcaggaagag cagggaacaa ctcctcttca cggagaggct
    15121 caggattcaa agtattctgt ctggtcagag ggaaggagca tacggtacca acatagataa
    15181 cacccttgat tttctccttg ccagccttac ggcaacaatc aaaaacatta gaattgatca
    15241 gattattgat tcggaacaat tcgccctggt tcttaaacac atagtcaatg ccagccacaa
    15301 catcagccag atgaactaca tactcagtcg taccaataac ctttttagcc tgctcataat
    15361 cagtcagatc agcattgaag aaatgagtat ccaggtcgat gacaggatgc ccatcttcgt
    15421 cgttcaggta ctccaacttt cctctccaca gattatcagc tacataaaca tcccagcagc
    15481 cttccttcac cagtctctta acaagattgg agccaatcat accgcaacca ccggtaacca
    15541 taattgtctt tttcatttaa cattcctcca aattttattc gtttccaaag aatttatctt
    15601 ccagcatcaa acaccaacaa tgatatatcg gcaagtgata ctcttgaatc atgtatgttt
    15661 caacttcagg agccttaaca caaacatctg ctaccgtggc aagttcccca ccctttgcgc
    15721 ccgtcaagcc aataactttt ataccgagag ccctagcaac aacagttgcg ctcataatat
    15781 tcttgctatt tccggacgtc gaaataccaa ggaatacgtc accagatcta ccaaagccaa
    15841 acagctgctg tgcgaagaca cccaaaccat cgacatcatt aatataagca gtagacaatg
    15901 cttcgtgtgc gaccaaagga attgccgtta aaccacactc gagatttttg gctaattccg
    15961 gacctcttac agggtcaatt gcaactaact tatcggcaaa ttccttactt acaggacgtg
    16021 gcgtcttaaa ccgcttcatc aactcgccag caatatgttc cgagtcagcg gctgatcctc
    16081 cgtttcctgc cacaagaagt ttaccaccat tcaaatagca ttcctccata attaggtatc
    16141 catcgataat gtcctgttta atcgtctcaa gtgtaggata tcgtttaatc aataagtcaa
    16201 tatgtttatt aagtttttcc gctaacattg ttactcctta tccattctgt ttttcaatat
    16261 aagtttccgg cgtgtagtga ataactctag ttccaccatt ttcaaattca aacggaatat
    16321 acatcaaatc acgcatagcc caacgaactg catcctgttt ctcaggttgt acatagaaaa
    16381 ccaagaaacc tccaccacct gcaccgagca actttccgcc taaagcacct gcagccatac
    16441 ccttttcata tagttcatca atgcttcctg tactaattgc tccaccagta ccttttttca
    16501 atctccaagt aacatcaagc tgtctaccga aatcatctaa atttctgttc ttatcagtaa
    16561 gaattgcctc agcttcatct acaagctcat acatcttttt cagtcttact ctcttatcct
    16621 ctgtgccact gacgttgtta gccttttgaa catcagaaga aaatctagtg aatccggtaa
    16681 agaacatcat cagattgttg ttaagctgct ttttacgttc aggcgaaata atgacaggca
    16741 gcacctcata accatccaca ttgaaattaa ttcgattgaa cccaccataa gatgcggcaa
    16801 tctgatcctg ccatccaccg gcttcattac acagaactct ttcaagataa atggcttcat
    16861 cagcaagttt cttcttgtca gcatacttac cctccagtgc ataaaaagca ttcaacatac
    16921 caactgcaaa agaactggat gtacccagac cagagcgagc aggtaaatca gcttcgtatg
    16981 taagccgaat ttcatgcata tcgagcattt tcatcgcatt acgaatcgca ggatgctcaa
    17041 tatcttcaat gctagttact ctctcagtac gagaataaga taactcagcg ctgtaatcaa
    17101 aaaatctagg cagatgtctc acattcacat aacagtactt atcaaaagta gtagaaagaa
    17161 ctgcaccgcc attctctttg aaataatcct ccatatccgt tccaccgcca aaaaaagaca
    17221 tacgaaacgg tgtttttgta ataatcataa atactctctc ctaatcttag tctctctgga
    17281 agatatcacg ggtatatact ttttccttaa tgtcatcaag gcaagcatcg tagcgatttg
    17341 ctataacaga atcacacatt gccttaaact gttccaaatc attcacaacc ttactgccaa
    17401 agaaagtctc tccatctttc aaggtcggct cataaatcac aacagttgca cctttagctt
    17461 ttacacgctt cataacgccc tggatagaac tctggcggaa gttatcagaa ttggatttca
    17521 tggtcaaacg gtaaacacca atcacaacag gcttttcttt gccagcatca tactgatttg
    17581 cttctgcgta attgtaataa ccagccatct gaagtacacg atccgcaata aagtccttac
    17641 gagtacggtt agattcaacg attgcctcga tcagattttc aggaacatct gcgtagttgg
    17701 ccagaagctg cttggtatcc ttcggcaggc aatagccacc gtaaccaaag gaagggttgt
    17761 tgtaatgagt accaatacga ggatcaaggc aaactccatt aatgatctgc tgagtgttca
    17821 agcctttcat ctctgcataa gtgtccagct cattgaagta gctgacacga agagccagat
    17881 atgtattggc aaacagctta acagcttctg cttcagtaaa gcccatgaac aatgtgtcca
    17941 cattctcttt gattgcgcct tcctgtagca gtgtagcaaa agtgtgtgca tcctcaacca
    18001 aacgctcatc atccatatcg gtacccacaa taatacgcga aggatacaga ttgtcataca
    18061 gtgccttact ttcacgcagg aactcagggc tgaaaataat attcttgcta ccagtcttta
    18121 cacgaacaga agcggtgtat ccaacaggaa tggtggactt aataaccata atggcatcag
    18181 gatttacttc catgaccagc ctaataacag cctcaacagc ggatgtgtca aaatgttgag
    18241 tgcggctatt gtagttagta ggagccgcaa taacaacaaa atccgcagac tggtatgctg
    18301 cctttgcatc caatgttgcg gtcagattca gttctttctc agccaagtac ttttcaatat
    18361 aatcatcctg gattggagat ttacggttat taatcagttc aaccttctct ggaatgatat
    18421 ccaccgctgt aaccttatgg tgctgtgcca acagagtggc aatactcaaa ccaacataac
    18481 cggtgccggc gactgcaatg ttatactgtt tcttcataat atttcctctt tccaccactg
    18541 ctgtggtcac tactttctta acaaaatgct gtttgtgaac agtaacctgc tatttatata
    18601 aaacagcccg aaagctgtgt tcattcatac ttaaaacagc cagctcctac gccggcaaat
    18661 tcaatttttc ataaatcccc tacccgttta gggttatatt gcaagtttga gtcattttta
    18721 ttgacctcgt ttggggttat aatacatttt cgcattgaaa tgtacctaca ttttccactc
    18781 aaccagtcac caaaaagttt ataaattcac ttattaccct ctattcttac tccaaaggta
    18841 tgtttaacat cgctttattg acctattttc accccaaatg gaagtttgtt tggtacatta
    18901 ttggtctatt cccaccccaa actgtctatt ttgagcgagt ttttgttttt tatcattgca
    18961 cattttcaag gctcaaattc taagcggatt tactggcagg gagacattgg taaatacttg
    19021 aacaatttca cggctttcat catcacatcc tacgataata attctaattg gataaagatt
    19081 atcattaata atctactttc acgtaaaaat tctgaactaa ataaaatcta ttgataatat
    19141 ttttttcttc cactttttgt atatcctaca ggtatagtgg atttaattat ccccaatgcc
    19201 tctgaagtaa cagacatcac taattcaata acacattcac taccatagtt tgtaggaaca
    19261 aaaatgacaa caaaatctgc ttccttatgt gatgtttttc catctgtcca aattttatta
    19321 ccaatgaaat ctagtttaaa atccctctta gaaactagtt cactctatat ttttattcag
    19381 tttcattagt tctgtttttt tgtcttcagt caccatatac atatatatct taattatcta
    19441 tccatttcat cctacgcatt cttgcgtaaa tacaattcac tctgttgagc tgatataaat
    19501 cattggtggt acaaagataa aatgataact gtttctatac ctccaaaaat tccagtaaat
    19561 atatctatag atagtttcaa aaaaaattgt cttagtttca taatatttaa gatagtggta
    19621 catactttat aacaacgtac ttttccaaat attattttcc gttatgaaca tccaatatat
    19681 tacaaagttt aaaagtttag atgaactata atcttcattt ctgcacagtg gataataaat
    19741 tcttatagat aaaaaatagc ctcttctaaa gtcacaaata ccttactccc acattttaag
    19801 taaacactct gcagcattct ctttgttata aaatgataga tattcctcaa ttttatctct
    19861 tattatgtta ttatcagttg ataccattcc attaatgttt cagaataatt acaatatttt
    19921 gggaccggat aactcccttt tgctcgtaac cactcatatc cgttaggatt cactaaaagt
    19981 ttaccgttta tttttcgatt ctttttttga accgtcccat gaacataccg attcaacaag
    20041 ctaaattata aaaattggtg tgctgtcatt attttttgga aaatttatta atggctatca
    20101 atatactttt gtatcatact aaatagctcg tcagttccta tatcggatat accaatatta
    20161 aagcatttag atcattatat ttaagagtgg tatcatccca aaatttcgaa tattttcttt
    20221 tatataagaa taatgatact gaattgcccc attcaccttt ctttttttag attttctaca
    20281 agtgattcaa accctccata attacagtta ttacttttga aacgataata tgaacatgtg
    20341 taatcttttt tattaacaat ccatcattct atccctaact aatatgctcc attgggcttg
    20401 acaataacat aaaacgtttt taaaatcaac ctcaagtcgt acattaaact tcgatgacta
    20461 atatactcta aatccaactc aaccatttta tggaaactaa cattatttct atcactaact
    20521 tgccacaaac ctgaacaacc aggttttaca tacaagcgtt gaagatcata tctcgtgtac
    20581 tcagctactt ccctctccaa aggtggccgt ggaccaatta gactcatatc gccttttaaa
    20641 acattccata gctgtggcaa ttcatcaatg cttttggcac ggataatttt acctatttta
    20701 gtgacacggg gatcttcttt catcttgaac attgcacctt ctatttcatt ttgatcaagt
    20761 aattcttcca atttttcttc agcatctaaa cacatcgaac ggaatttata cattttgaat
    20821 gtttttccat ttttaccaac acgtgtatgg caaaaaaata tcggacttcc tggctcttct
    20881 tttctgattc gataagctac atagaacatc aaaggagaag ctaaaatcaa accaataatg
    20941 cttcctacaa tatcaatcat tcttttaaaa aataaataga tatatcgttt ttcaactttt
    21001 tctttatcca accaaacttg attaggatgt acttcactaa tacgagataa taaatcttca
    21061 ctttccatac tttccctctc tcctcatttc ctttttatct acgaataaag aactaaaata
    21121 aaccaaactt cttctttttt acctcttcat agactggata actgatttca tccccgttga
    21181 tcagatcctt tgcgacttgt ttcatctgta ctactttttc cttaccaaag tccttagcaa
    21241 tctgctcata gcactctttt aagtaaaagg tacgcttaga gaccccatgg gcatcagaag
    21301 cagccatctg cacaagatta tgtttgacca tcattttggc tgttttctgg atgtcttttc
    21361 caaatacacc gacataactt ggtgcggtga gttgggccaa gcatcccata tctaggaatg
    21421 gcagcatatg attcgggtct ttacgaaaat gcgcgttgcg ttctggatgg acgatcactg
    21481 gtgtctttcc tagttgtaac aactcaaaaa agagctgttc tgcatataac ggcacttcca
    21541 gtgtcggaaa ttcgattaga agatatgtat catctaaatc tgtaaataaa atttcatctc
    21601 ggtcgatgtc ttcgatcagt gttccggtaa tacgtacttc ttgtccttcc aataacgtca
    21661 aaggcaattg tcttttctct aattctgctt gtaacgaagc gactagagaa atgacttgtg
    21721 atttttcatt gctgtatttt ccattattat gatggggtgt gcataaaata tgtgtgatcc
    21781 cttgttgaat cgctttttca gccatggcga tactggcatc tatgttttct gctccgtcat
    21841 cgattcctgg caggatatgg caatgtagat cgatcatttc tcttgtctcc tctcttttta
    21901 tttcttaatt cccgtagtaa tagccagcat ctttgctgtg ttctgcccca ttgtatacaa
    21961 caccaaggac tcgcgcttgt accatatcca gcaattcttt tgctttagtc aatgattctt
    22021 tccgtgacat attttcacgt acgaccaata aggtgccatc cgttttggaa gacatgatct
    22081 gtgcatctgt tacggcaact actggcggca tgtcgaagat cactacatca aatagttgtc
    22141 ttgcttctgc taaaatctga tccattcttg gcgaacttag tagttctgat ggatttggtg
    22201 gtttaggtcc acttggcaga atcgacaaat tctcgactgg tgttcgttgg atctcatctg
    22261 ctacacttcc tgatgaacta agaacggtac ttaatccgct tgcgttgttc aacttgaacg
    22321 ttttatagat gacgggctta cgtaagtctg catcgactag cagcacccgt tgaccagatt
    22381 tggcaaaaac aacggcaatg tttgctgcag tggtcgattt cccttctcca ggtcctgatg
    22441 aagtcacaac aatcgtttta atttgctgac cagctgcgga ggcaaactgg atattcgtcc
    22501 gaatcgtacg atattgttca gcgattggtg aagaggggtt tgtgagtgtg accaaactga
    22561 ctgcatgagt ttgggctgct ttttgtttct gtgttcgtgc catttttctt tcctcctaga
    22621 ctcttgagcg gcttctgcgt gatggcgctg ttttatcgtt tgtagctttt ggttgtgtcg
    22681 gagctggtcc ttttttacgg ataccagcat tcaattcttt cgctgacatt tctggtacag
    22741 tccctaagat cgggaagccc aatgtatccg tcacatattt ttcatctttt actgtccggt
    22801 cgagtaattc aaataagaaa gctagaccta cgcctaccat cattcctaac actaagccaa
    22861 tcactagatt caatttatta tttggcgaaa caggtgtcgt attggcaact gcacctgaga
    22921 tgatcgagat tttatcgaca ttcatcacgt ctttggcgtt ctcttggaat acttgtgacg
    22981 ttgtattagc gatttgttga gccgtcactg catttgtgct tgttgcttgg atcgagaaca
    23041 tttgtgaatt ttgtgactga ttgacggaga tggcatcttt gatttgtcca gcagtcattt
    23101 ttacattgtc ttctgtttct aaacgatctt tgacttcatt tagtactaga tcactgacga
    23161 tcatatcctt atacgtattg atcatttgca ggttcgtatt gacatcattg acatttgcag
    23221 tatctgattg cggcagtgtc acgatcaatt gggcttgtga gctgtatttt ggcgtcaaga
    23281 tgaaaaacgt cacgatgcct gagatggcca agccagcaaa cattgagatg aggattgtta
    23341 tcacgtgttt gcgtaatgtt tcaaaaattt ctttcaagct gattgtctct tccatttttt
    23401 ctccactttc ctatttattc tctagattca tttgatcttt taattctttt ttcacacgat
    23461 caagttcttg atcgctgact ttttggtatg agatattgtc ttgcatgaag ccttctcctt
    23521 gcagctgatc ttgcttgatc gtaccaaatg catcccgata atctaaagca atctttttca
    23581 tttcatcaaa gctcatgtct gttttcatat tcgactctag agcattcaaa acggattgat
    23641 agttgcttac accgttcaga ctcaacactt ttttcgctac accttctacg atctttcttt
    23701 gacgttcttg tctgccgtag tcaccatttg gatcttcata acgcatacga gaataagaca
    23761 atgcctgatc cccgttcaat gtgattttac cgatcgtaaa atcatagcca tcttgtgaga
    23821 aggtgaggtc attattgact tcgataccac caactgcatc aacgagttct tttagacctt
    23881 tcatattgat tgaaacatag tgatcgattg ggatgtctac atatttttgt acggtatcca
    23941 tcgccatggc agctccgccg aatgcgtaag cgtggttgat cttgtctttt gtgtcatgtc
    24001 caacgatgtc gacatacgta tcacgaggaa tgctgacaat cgttgtctgt ttgtcttcag
    24061 gactgactgt ggcaaccatc atcgtatctg atcttccttg atcgacccga cctaaatctc
    24121 ccgtatcaat ccccatcaat agaacagaga aagattcttg tttgtccaga tcaactggtt
    24181 ttgttcgttc tccttgggtt cgttccactg attcgtaagt tttttcaata gaatgtgaga
    24241 gatctagata taattttgct cctactccta cgattgctaa tactaatacg acaagaattg
    24301 ctaaaataat tagaaatatc tttttccctc ttgatcttct tcttcgtttt gccatagttt
    24361 cctccgtttg ctagccttgc ttgaattctc gcttattaat agctcgtatt gctatagccc
    24421 atttgctgag cgactgtttc tgcttttgac gcgtatgcgg ctcttatttt ttcattccgg
    24481 atctgttcga tgagactgag tgcggtcaaa tattgatcgt aagtagcagt agctgttact
    24541 tgaccgtctt tcagcatcgt atcgaatagc tgatctagtt gcgtgacttg cgcgatctga
    24601 gtatcagcga atcctagata ttgcataaca accgttttcc aaggactatc tttaaaaaag
    24661 ctcatatttt cacgtacacg agcgacatct gcacttgtca ccttttcttt gatgacgaca
    24721 tcgtctacag cttgctgcca gtttgataca tttttagtaa ataaatcgtt gacagctgtc
    24781 tggatcttca gttttgattc tgcatcctct aactgcgtta agactgcttt cttttcttct
    24841 tggatcgcag cagcttcttt aggtaattct gattccttga tgtcataatc atctgccgaa
    24901 agtttgatag aagatagttt atgtgacaaa tttgaaactg tttcttctgt tatttcagga
    24961 tttaggaaaa ttttttcttt tgtactgtac agcttatcta cttcttttga taaagaggtc
    25021 acggtgtctt tttgattggt gatggcagat tctgcgtaat tgatccgttg atgatgttgc
    25081 cgctgattat tgaaatggta tccaaacaag caaatcacaa tagcgcctgt gcctaccgcc
    25141 acagtagcca ataacttgac gaacggcttt tccatagatt cactcctaaa tattgatgat
    25201 agataaataa agatgtgatt cattcccatc agaatgagcg ctttttattt taaaagcata
    25261 attaaactaa tatattcata gaaatattta taaacaggtt ttccctatat aatcatacca
    25321 caaaaagttt tcttgactag ggaaattcct aatttaaaag taaataaaac agtaacaaga
    25381 tcatgattaa tcgttttcat ttttatttcc tctcttttca tgagcatttt gaaaattcga
    25441 acaaagaata aaataacaaa aattattaaa atagaattaa taacagctat ttatatttat
    25501 aacaaaaaaa cagagacaca gcacgtttcg tgcatgctct ctgtttttca aacaaaagac
    25561 tattttgtga tgatttgatc caagcttgct aatgacaaag ccatggcagc tatttgacta
    25621 agttgtgtta gtcggttctt acgtactgct ggttcatcag ccatgaccat cgtttgatca
    25681 aaataatttt cgatcagtgg acgaagagcg acaagctggt cgtagttttc gtggatcgtt
    25741 tggtcagcaa aacttgcttt cattgcttct actgctttgt gtaattcttt ctctgcgtcg
    25801 ttttcgaaca gctcaggatc tacttctgat tgtttttcaa gctgcgcttt tttcgctaag
    25861 ttcacgacac gtgtcaaagc ttccattgaa ggtttgaagt cttcatctgc aagatgttgt
    25921 ttgaacaatt gtgctgctgc aaatactttt gtcagatctt tttgttccgc attcaaaaca
    25981 gcatcgatga catcatgacg aatattcttc gttgtcaata attggcgcag acggcctttg
    26041 ataaagtcga tcacttgttg ttgcccttct gataaaccaa tgccgaaacg atccgtatct
    26101 ttactgataa catcgctaat cgtttcttgc agaacagaca aaggaaaggc ccattttttc
    26161 gattcgatga tccgaacgac accgtatgct tggcgacgta aggcataagg atcgtttgaa
    26221 ccgcttggga tcatcccaac agcaaagaaa gaaaagatgc tatctaattt atctgcaatc
    26281 gctaaaacag aacctactgt agactgaggt agctctcctt cactggatat aggcatgtaa
    26341 tgctcacgaa tcgctgtcgc tacagcaggt ttctcgcctt tcagcagagc atatttttcg
    26401 cccatgatcc cttgcagttc aggaaattcc cctaccatgt tggttactag atcaaattta
    26461 tagatttcag cagctcgttg cagatccatc aattcttcct cagatagtcc tacttgttcg
    26521 ccaatgatac gggcaatgac ttgtacccgt tgcattttct catagatact accgattttt
    26581 tcatggaaag taacagtttt tagtttttca acgtattggt cgattgaagc tttcttgtct
    26641 tcattataaa agaattccgc atcttctaaa cgagccgtca atactttttg attgcctcga
    26701 accacatttt ccaattttac ccgatcacca ttacgagcag aaataaaatg aggaagcagc
    26761 ataccttgat cattccgtac ctcgaaataa cgttgatgtt ctttcataga agtgactagt
    26821 acttcttcag gaacagaaag atatttttct tcgaagtcgc cagaaaagac agttggatat
    26881 tcgactaaat tgttgacttc ttctagtaaa tcttcgtcta gatcaataac ccaatgattt
    26941 ttttcggcta attcattggc ttgtgtaacg atcatttctt gtcttttttg cggagacgca
    27001 atgacgaatt gttcattcaa tttttcttca tattcttttg catgcgcaaa tgtcacatct
    27061 tcgcctaaga aacgatgacc tcgagaggct ctgcctgttt tgacatccaa gatttcgaat
    27121 gggattactt cgtcatctaa caatgccacg atccagtgaa tagggcggat atattcaaaa
    27181 tcgtattctg cccaatgcat cgttacaggg aaagtcaggc ttttaatgac atctgctaaa
    27241 cctgtcaata cttctttgga ttccttacca tgagtgtatt tcgttacata gacatattcc
    27301 acacctttta attctctaaa agtgatagcg tctgtcgtca gaccttgtcc gcggacaaat
    27361 ccttccgctg ctttcgtcca gtttccatct gcgtccaaag caattttttt ggctggtcct
    27421 ttgacttctt cttcactgtc cgcttgtcgt tctggaatgt ttgttacttt gattgctaaa
    27481 cggcgcggtg tggaaaatgt ttcgatcgta tcataagtca attggttttc atctaaaaat
    27541 tttgctgttt tttcttctaa ttgtttcatg cttggtgtga caacatgagc aggcatttct
    27601 tccagcccga tttcaagtaa taagtctttt gccatcttat tcagcctcct tcacgttttc
    27661 tggatttgct gtgtttttat tcaacaaagg gaatcctaat ttttcgcgtt ctgccacaaa
    27721 gattttggct acagcacgag ccatgttgcg gatgcgagaa agataaccgg cacgctctgt
    27781 tacggatact gcaccgcggg cgtctaataa gttgaatgta tggctacatt tcaagatata
    27841 atcatacgcc ggatgaacaa gattttcatc gatacagcgt ttagcttctt tttcaaaacg
    27901 atcaaagttg cttagtagca agtcttggtc gcttacttca aaagaatatt tagaatgttc
    27961 atattctggt tgtttgaaga tctcgccata tttcacgcca tcagcccatt ctaaatcata
    28021 gacactttca acttcttgga tataagaagc aagacgttct aatccataag tgatctcaga
    28081 agttactggt ttacaaggca gtccaccaac ttgttggaag tacgtgaatt gtgtgatttc
    28141 cattccgtca agccatactt cccaacccac accggcacag cccatggatg ggttctccca
    28201 gttgtcttca acaaaacgga tgtcgtgttc tagtggatcg attcctaaaa ggcgcaagct
    28261 ttctaaataa agctcttgga tattctcagg tgaaggtttc atcacgactt ggaattgatg
    28321 atgctgatac aaacggtttg ggttttcgcc ataacgtcca tcagctggtc ggcgagatgg
    28381 ctctacgtaa gcagcattcc atggttctgg tccgattgcc cgtaaaaagg tatatgggct
    28441 catcgtacct gctccttttt ctgtgtcata cgcttgcatc agcatgcagc cgtttgatga
    28501 ccaaaatttt tgtaatgtta aaatcatatc ttggacggtc aattttttac tcatctgttt
    28561 tgcccctttc aattttaaag cgttgctgtc aaataagaat acaggaaaga aaacgacaaa
    28621 aaatccctat gtctgattac agacataggg acgaataatt cgcggttcca ccctacttcc
    28681 gaataaagat tcggcagctt catttatcgt actcgcaagt gcctcttcaa aaatcgtttg
    28741 ggtccggctc ccactctccc gaactcgcta aaccacgtgt tttttactga tcttggtcaa
    28801 tgtacgtatt cattttgcag ttttcttgtt tctataatag atcctctcag tgaacgatct
    28861 atttgatcta cggttaataa tagacggaac tccctgtgtt tgtcaaatgg ttttgatttt
    28921 ttgttgtatg tttcatttac tattcttctt ttttaggtag atctttttca gacatgtctt
    28981 tctctgacgt ctttaatacg tgtgcccaag acttcatttg atcaatgaac tttttgcttt
    29041 tcaaatggat accgacatat tcatcataca gctggtcgat gagttcacgg attgcttgtt
    29101 tggtttcttc tttcagatgg attccactga ttttatcata agaaatagct gaaaacatcc
    29161 gaacgaaata aacagctcga ggatctgcat gataacggtg atcatccatc gaccagtgtc
    29221 tttcgcatat cacaccacca tatttggaag aatagtcaaa ttttcctttt gtttccccac
    29281 atacgacgca gtgtgtccag attggtgcaa ttccgaagcg ttgcagtatt tggacttcaa
    29341 aaatattcgt gatgatttct gcatcatctc cttggtccat cagttgcagt gcttgttgcg
    29401 taaactggaa caaatgcggg tcgtactgat gatcttcgat tgccgcatct actagattca
    29461 ataaatatgt gccatagccg ctcaaaaaga tgtcttcttg gatgtgtcga aacgcgtgga
    29521 catctttgac actgcttaaa aatgacaaac cttcacttcg aaaattcccg atataaactg
    29581 cttcagtaaa agggagcaaa gccggagcta agggctgatt ttgccgatgg atccctttga
    29641 tataaaacat cattttgcca gaagattccg taaagatttt gactaattta tctttttctt
    29701 tgtaattacg gctagataaa atcaagcctt ttgattctgc aagctggctc atattctccc
    29761 tcctaaaaaa taaatctgga aaagcagatt ctgccgatcc agatcattta tgttgcgatc
    29821 ttccgcagct tttctcaaca cgcggatttt tatccagttt atttctatac ctaacttaca
    29881 agaagctcag aatatcaata ttcgtctttt cggtagccga agtcttgcag atagactttt
    29941 ttgtctcgcc agtctttttg aactttgacc caaagttcta gaaagacttt gtctcctaaa
    30001 agattttcga tatcttttct cgctttcgtt cctacttgct tcagcatttt tccgcctttg
    30061 ccgataatga tccctttttg gctatctctt tcgacaataa tcgtagcctg gatatggatt
    30121 ttatcattct cgtttcgctt catcgaatca acaacgactg cgacagaatg aggcacttcg
    30181 tctcgtgtta aaaacaagac tttctcacgg atcaattcag aaacgatgaa gtattcagga
    30241 tgatcagtca cttgatcatc tggaaaatat tgtgggccct ctggcatttc actgactaat
    30301 acatccatca aacgttcgac gttgtttcct tctgtagcag aaattggaat cacttgtgca
    30361 aacggcatct gcgttgaata atcttcgatg attcccaaca actcgtctgg atggatttta
    30421 tcgattttat tgataatcag ataaacagga gaatgcacgt tttttagacg ttcaataata
    30481 aaatcgtctc cccgaccgcg tttttgatca gcgctgatca taaataagac cgcgtccact
    30541 tctttcaacg cactgtaagc tgcttctacc ataaaatctc ctaaacgatg ctttggttta
    30601 tggattcctg gtgtgtcaat aaaaatcaac tgagcttctg gtgtagtata tactccttgg
    30661 attttatttc tagttgtctg cgccttatca ctcatgatgg caattttttg acccacgatt
    30721 cgattcaata atgtggattt tcctacattc gggcggccga cgattgcgac aaaccctgat
    30781 ttatgttctg gcatatacgt tctccttttt aattaaacaa caaatgatat attttaggga
    30841 caaataaaat caacccaacg atgacggaaa aacaagcggt cactaaaact gctccagctg
    30901 ccatatcttt gatttttttc ccaatcggat gaaaatgaaa atccgtaaac atgtccacga
    30961 cattttcaaa aacagtattg atgatctcta ctatccatac gagaaaaaca gctaaaagca
    31021 gccataacca ttctatccga tcaagctgga aaatcacgcc cataaacaaa gcgaggattc
    31081 cgaacgcaac atgtttcctc atgttgcgtt cttcctcaaa aacggttttg actccttgga
    31141 cggcaaattc cacggacatc aggaaatgtt tgtttttctc tacgttttta tcttttaagt
    31201 ccataggcat ctaagatctc tttctgtaaa ccaaacattt ctttctcatc ctctggtgtc
    31261 atatggtcat acccgttgat atgcaagaat ccatgaaccg ctaaaaagcc aagttcccga
    31321 tcatacgaat ggccgtaagc ttctgcttgc tctgcagcac gttcagtaga gatcatgatg
    31381 tctcccagta cacgaggcaa tggattctct ccatcttcaa aaatcactgg gatttcgtct
    31441 tcgccctctt cttccaaggc aaaactgatg acgtctgtcg gtgcatcttt tccacggtaa
    31501 tcacgattga ttacttggat cgctgcattg tccatgaaag taacagacat ttctgtttct
    31561 tctggcaact ctaaatatga tgctgcaaat tgcagcaagt cgtcgatttc tttcattttc
    31621 tcttctgaga cttggtttgt ttcatccatg aaagtaatat ccattataca tcgcttcttt
    31681 ctattcttct tgttcttctt ttatcttctc aacttccgat ttcattttag gatatttgat
    31741 ccgagaatgg aaggtactgc ttaaaccgct gatcaatgat ttttctactt tacggatttc
    31801 ttttaacgtc aatccactgt cgtccaattg tccatcggaa atacgctctt caataagatt
    31861 atggacaaaa ctttcgattt tttcgctcgt tggatggtcc attgctcgta ctgccgcttc
    31921 acaactgtca gctatattga cgattcctgc ttcacggctt tgaggttttg gtccaggata
    31981 acggaattgc tcttccgtga cttccgggtt gcgttccttt gccttaacgt agaaataacg
    32041 catcagcgtg gttccatgat gctgtctgca aatatcgatc accatttgcg gcatattata
    32101 atcttccaat attttggcac catctgtcac atgtccaaag ataatatgtt tactgtcttc
    32161 tggcaataag aaattgtgcg gattttccgc tcctgaaggc aagttctcaa cgaaaaagct
    32221 ggcgtgtttg atcttgccga tatcatgata atagcaagct actcgagtta gtaaagaacg
    32281 tccaccgatt tctgctacag cattagcact tagattggct accatcatgc tatggtgata
    32341 cgtgcctggt gcttcttcca gcaactgttt caataaagga tgattaggat tgctgagttc
    32401 atttaatacg atcacactat catcattgac catcaattca atataggggt gcagacctat
    32461 cgtcagcaag aatgagaaca gtgcacctgt gaatccgcat actagcgtta accaagtcgt
    32521 tgcatcccca aagctcattc cttggtagat aatcaaaatg acatccatca tcacaggaaa
    32581 aataatgacc cacatagcag cggataaccc ttgttctgag atccgtttgc gtctgacgat
    32641 cgttgccatg agaccagaaa atacatagga catcaaaata actgtcagaa agttcgtgcc
    32701 aatcgaatca taaaaaatga aaagtgcgga tactgcttga aaaataccag ccatgatccc
    32761 tgctcttcga ttcaagaaaa agcttaaaac aagtggagca aaagccgcgg ggtaaaacag
    32821 cggaatataa gcagcttgtt ctgtttggaa gagttggaaa aatttcatta gtacgacgct
    32881 tactgacatt gccgtcacgt agaaaagtac gtattttgtt ctttctcctg cattttggaa
    32941 ttgaagtgaa ttataaatca gtacaccgat ttgaagcaaa acagctaaaa ccatggcaat
    33001 gagtgggaaa atagaagtcg tttgattcgt caatcctagt aattccaatt ttttcatcgc
    33061 tgaagcatca atttgatttc cttcgcgaac aataatttct ccttggaaga tcatcactgg
    33121 ttgtacagct tctcgtgcag attgttttaa ttcttctgtc tttttctcat tcaatacatc
    33181 attaactaca atgccttgat ccactaagta gcgaatcatt tgttgctgct ctgttgtgac
    33241 attcaaatat tgaatctgat cttctgcttc ttgtttaaaa gcttccaatt cgctttcacg
    33301 aacttgttta ctcatctgtt cgtcaatgag cttcaaactt tcatccctaa ccgtgtgaat
    33361 ttccgtttca gtcatttcaa atatcgtcga ataaaaattg ttcggcagtt tttggtaaaa
    33421 cgcaacgttt tctgcattca cgttttcaaa atttttcttc agtgtagcaa tccgctcttc
    33481 tgtagtaggt ttcgggatat tttcatcttc cttcgcctta tctaccttgt cattataaga
    33541 tttgttgatg gtatcattcg tcttatcgat caattcgaat aattgcttga tccgattgtt
    33601 ctggtcatct gccagatctt tttgataagt atattctgga gtcactgctt ctgctgccag
    33661 ttttcttctt tgttcggttt cttccgtgtt ctcaatcgtt ttattcgcac ggatactctc
    33721 ttctgctact tggccttctc tgaaatcatt ggccttttgt ttgacactac taaatgtaac
    33781 gccgcagaca atgataaaaa acaagaacag aaggaaaggc aatgctgcct ttccgagttt
    33841 tttttgtagt ttctgcagtg ttttattcaa catgtgctta tcctcctaag caaagcttat
    33901 tcttgatgaa gatccgcctt ttcataagct ttgatgattt cggcaactac agggtgccga
    33961 acaacgtcac ttgcttcaaa attaacaaaa tcgatttttt tgatttgctt taatgttcgt
    34021 tctgcatgaa caagaccact tgtcgtccca cgaggcaagt ctatctgact tgtatcccca
    34081 ttgacaatca tttttgattg ataaccgaga cgggtcaaga acattttcat ctgagcgact
    34141 gtcgtgtttt gtgcttcgtc caagataacg aatgcatcat ccaatgtccg cccccgcatg
    34201 taagctaaag gtgcgatttc gatcacgcct cgttccatca atcggttcgt atgatccaat
    34261 ccaaaaattt ggtataaagc atcataaaca ggccgcagat aaggatccac tttttctttc
    34321 agatccccgg gtagaaagcc taggttttcc cctgcctcca cagcgggccg cgtcaaaatg
    34381 attttttgca cttcgccttt ttttagggca gcaattgcta atactacggc aagaaacgtt
    34441 ttccctgttc cggctggccc tacaccaaat acgatgtcat gtttggctac cgattcaacg
    34501 tatttctttt gaccgctgtt ttttacccgt ataggttttc cattacggtc tttgacgatc
    34561 tcttcctcat acatctcaac aaaataatct aatgtacctt tttgccccat cttcaatgcc
    34621 gtcacaacat ctggcgtact tacgtggatt cctcgtttga tcaattcctg taatgtccga
    34681 atgactgagg cagccaagga aatctgggat tgctcaccaa tgatctgaat gatttctcca
    34741 cgtgtatgga tggttgtttc cgttgtttcc tcgatcagtt ttatatgctt gtcgtgtgca
    34801 cctaaaagca tgctgacatc atcagcagct gtcaatctaa tctctaaaga actagactct
    34861 tccgtcaaaa atagacatcc cctttttttc gattcattct ttcctatttt accatactca
    34921 gaagaaacta tatagcaaat aagaaaaggt tgtaaaagag tcgtttaacc atacgtacca
    34981 agaaacgtta aaaaatagcc gtttccattc gctaaaggtc tcgttttgat aaaaagaagc
    35041 tgagaatgta agttatatga aagtggagaa caaaatattc cgtttcattc agcaggtatt
    35101 ctctaggtat ttttcttgtt tcaaaaaaag aaaacaaaat agttaaaata ttcttgcatt
    35161 tcaaaaaaaa caggtgtatt attacgtcaa caaataaaca tacagcaaag cgtcgaggaa
    35221 aacagtaggc tattcaacga tttattccca gtgagtcatg taagtgagaa atgacaataa
    35281 atcgaataga cgaacatcat ttccgagtca atgtcgaaaa cgtagatcat tgtggctgcc
    35341 tccattatca ggctagcgga taactttatc aaaaagcgtc cgtgaatgaa gaagcttttt
    35401 taagcgaaaa aaggtggtac cgttctagaa tctagagccc ttttcccaag tggaaaaggg
    35461 ctttttttat attttaaggt ggtggttttc atggaatctg atattagtgg cattactaat
    35521 tgctgcaacg tttatatcgt caatcaaaat gataattatt ggaggaaaaa tttatggaag
    35581 aaacaaaaac ttattcatgg aaacaactat tgatggtcag ttcacttatc ttcgggatgt
    35641 tcttcgggtc aggtaatcta atctttcctg tccacttagg tcaaatggct ggcgcaaact
    35701 gggtcagtgc tggcgtagga tttgccatct cgggcgctct tttcccatta ctagctatct
    35761 tagctgtagt agtgacaaaa agtgatggtc tgtatgactt agctaaacct gtcggaaaat
    35821 ggtatgctgc cttgttctta atccttgttc atttgacgat cggaccattt ttcggtacac
    35881 cacggacagc tgctactgct tttgaaatgg ctgctaaacc atttttgcct gaaaaatata
    35941 attcattagg tatgttttta ttctctgctc ttttcttttt acttgcttat ttccttactg
    36001 tccatcccac acggttggtc aaatacgtcg gaaagtatct aaactccttg tttcttgcat
    36061 tgttagcaat catttttgtc gtttctttat tccacccaat gggaaatcta aatcaatctg
    36121 ctgcagatac ttaccaaaca aacgctttaa caaatggtgt cctagaaggt tataacacgg
    36181 ttgatgcagt agccttgctt gccttaagtg tcacttttgt ccatgcagtc cgtgaccttg
    36241 gtttcaaaga taaaaaagtc acagaattaa cagcaaaagc tggtagcctg agcgttgcgc
    36301 tagaagtcat gatctatttt ggtcttgttc ttctcggtgc gatgagtttg aataagatgt
    36361 ctttatcaga aaatggcgga actgcacttt ctcagatcac taatttttat ttaggtaact
    36421 tcggcgctag cttcttagga gtgatggtca cattaggtgt cttcactacc gctatgggac
    36481 tcgttgtttc ttttgcgcaa gatttccata aactgttccc taaagtcagt tatatgacat
    36541 ggctgcggtt aacgactttc gtctcttttg tcgttgcaaa tgctggttta gacaatatca
    36601 tccaatggtc tttaccagta ttgatgcttt tgtatccatt gtcgcttgcg ttgatcttgc
    36661 tttccttaac agcaaaattt ttccaaaaga caccatttgt ctatcaagta acgatgttgt
    36721 ttgctgctgt tcctgccgta ttggatatgc tggcaaattc gcctgcttta gtgagtcaac
    36781 aaagagttgt agcaagcatg ctcgaatttt atcatcatca cgttcctttt gctgcattag
    36841 gacttggctg gatggtccca acgttacttg gttacgctgg cagcctactg ttttattacg
    36901 cctatcgcct atcagggtat aaacaagaag caaatgaact accagaagaa taaccaaatc
    36961 aaacacaatt taacataaga aatattaaac aggaaaaaac cagctagatg aaagaatcct
    37021 attctttcgt ctagctattt ttttgttctc ttttttacgc gcacaaaaaa actgcggaca
    37081 atgattgtcc gcagtttaaa tgataaaatt aagattgtaa tagttctttc acaatggcat
    37141 ttacttggtt tccatctgct tttcctttga ctttaggcat gatcgcaccc atgactttgc
    37201 caaattcttt cattgatgaa gcaccagttt gtgtgattgc ttcttgaaca agttgtcgaa
    37261 tttcttcgtc ggaaagttgt tccggcatgt atttttcgac tatcacaatt tcacttttta
    37321 ctttttcagc taaatcgtca cgtcctgctt cttcaaactc atgcaaagaa tcacgtcgct
    37381 gtttcatttc gcgagacaac accgttaatt cttcttcttc tgtcaagtcg tgaccttctt
    37441 taatctgctc gttttgtata gatgacttga tcattcggat gacttgtaaa gattccttat
    37501 ctttcgcttt catcgctgtt ttcatgtcat cgtttaatct acttagtagt gacacactac
    37561 caactccaat tcatcacaga actagaattt tttgcgtttt ctagctgctt cagatttttt
    37621 cttgcgtctt acacttggtt tttcgtagaa ttcacgttta cgagattctt gtaaagtacc
    37681 cgcttttgac acggaacgtt tgaagcgacg aagagcatcg tcaagtgatt cgtttttgcg
    37741 aacgactgtt tttgacatgt taattccctc cctccgagct taaaaagtaa ccgtaattgt
    37801 tattgaaata tatctcaaaa aacaagactg tcctgtaaat attatatcta atgctgtttt
    37861 gaccgtcaag gaaatcttgc ttttttttaa aattatcaaa tgttatttta tttaaatcct
    37921 tattgacaat tttcacatct tccgaaaatc tcgaatctat gcccttcgat cgtacatcca
    37981 ccgagctgtt cttcgaaata attcattgga cacatatgga tttctttcgt tgttccgcac
    38041 accgtacaga taaagtgatg gtgatggccg atttcccctt gacagcaatg aaaacgaaac
    38101 ttcatctctc catttaaatc ggtttcttcc agtaaatcga tttctgcaaa atcatggaga
    38161 tttcgataaa tcgtatcgta acttactcct ttgaattcct gattcatata gtcaaaaact
    38221 tctttagcgg atacgtagcg attgcgcttc actaaataag ttaacaatgc ttctcttttt
    38281 ttagtatatt taaaaccgtt atttttcaat gtttctagtg attgttcaat caatgtctgt
    38341 tttttcatga cttattcctc caaatcaaaa tgattacgaa gtacacattc tagagtatgg
    38401 tataataaac ataaaaaaaa tgcaagtgag gttttatcca tgtcttccga ttctacagaa
    38461 atcgttactt tttttgtcat ctctgactct gctggagaaa ctgcgacaaa attagcgcaa
    38521 gccactatgg cccaatatcc taccgttgag ttcaatttat tccgcagaac atttgtgaca
    38581 gatgagaaaa cattgatgca ggcactgcaa gatgcactaa aagaaaaagc ccttgtcctg
    38641 cacacattga tcaatcaaga attgatcgag atgacgcgtt ctttttgtga agaacatcaa
    38701 ttatttagtt ttgatgtgat gactccgccg atatcagaaa tcgagcgctt aactggtatc
    38761 aagcccattc ggcaacctgg cgcccttcat ttattgaatg agaactattt caaacggatc
    38821 aaagcaatgg aatttgctgt aaaatacgat gacgggaaag atcctcgtgg gtttctagaa
    38881 gcagatgtcg ttttgctagg cgtatccaga acttccaaaa cgcctttgag cttgttttta
    38941 gctaacaaaa atttaaaggt tgccaatcta cctttgatcc cacaagcaca tattcccaaa
    39001 cagctttggg agatcgatcc taaaaaaatc gtgggactga cgaataaccc agatatttta
    39061 aacaatatcc gtaaagaacg gatgcgctct tacggattaa atcctgatac tgcttattcc
    39121 gatatcgaaa aaatacgagc tgaactcgat tttgcgaatg atctttatga aaaattgggc
    39181 tgcgtagtaa tcaatgtcgc ttctttatct atcgaagaaa cagcttcact tattttgaat
    39241 gctttagacc tagaagatca tagttattat ggaactgaaa ccaccgaaga aacagactaa
    39301 cagtttttct tgtctttttc ttaaaaaaac tggactgtgt cagcgaagaa gattcgttga
    39361 tacagtccag tttgtctcta tttgcaccta tcgttattga tgttccacat gtttgagcgt
    39421 ctggttcaaa atatccagta cgtgagaatc atcaagacta tacaaaatcg cttttccctc
    39481 acgatgggac ttgacgacat ggttttctct taataaacgc agttgatgcg atacagcaga
    39541 ttgttccatt tctactgctt gggtgattgc tgtcacattt tgttccccgt ctttcagata
    39601 cagcagaatt ttgagacgag tggggtcact caacattttg aatagttggc tgactttctc
    39661 tacttttgtt ttttgcgtgt ctggcaatcc attcacctcc aacttgaaaa aagactgact
    39721 tctaaagttt accacttttt ccatcagtct caagtttttt tattcttctg tcattttttt
    39781 gataatcgct tcataagcgg ctactttttc agctgcgtct tctgaagagt ttccttttac
    39841 accgatataa aatttgattt tcggctcagt tcctgaagga cggatagcga tccagccatt
    39901 ttcttccagc gtatacttca atacgtcaga tggcggtgtc gttagttttt ctacgtttcc
    39961 ttcacggtct gttctcgtca actctttgaa gtcttctgtt tggatgactt ttgctccggc
    40021 aaattctgtc ggtgcttctt cacggaattt ttccatcaaa gcagagattt ttgcagaacc
    40081 ttcttgtccg ctcattgtca cagaaatcgt tttttctgcg tagtagccat atgtttcaaa
    40141 aatcgtttgc aagccgtcat acaacgtttt gccttgtttt ttatagtaag ctgctacttc
    40201 tgctaataag agcaatgctt gaatcgcgtc tttgtcgcga acgaaaggtt taactaaata
    40261 accgtaactt tcttcgaatc cgaacataaa agtatgggaa tgatcttctt caaattgttg
    40321 aattttttca gcgatgaatt tgaatcctgt caatacattg aacatggtag ccccatagtg
    40381 ttccgcaatt gctgtaggca attcgctaga aacgattgat ttcaataccg ctgcattttc
    40441 aggtaaagtg cctcgcgatt ggtgtgcttc caaaatatat tgagcaaaca gggctccgat
    40501 ttggttccct gtcaatactt cataggagcc atttggcaaa cgaacggctg cgcctaaacg
    40561 gtctgcatca ggatctgttg caatcagtaa gtcagcgcct tctttctcgc caagtcgcat
    40621 cgcatactcg aaagcagagt gttcttctgg attcggtgat ttgactgtag tgaaatccgg
    40681 gtctgcgaca gcttgttctg gaactagtac gaattgttcg aatccagctt gtttcaacgc
    40741 acgttcgccc aacatttttc ctgttccgtg aagtggtgtg tacacaagtt tcaaatcttt
    40801 acccattgtt tccaccaatt catgatcaat cgttactgct ttgatttctt ctagataagc
    40861 ggcatccact tcttctccga tgatcgtgat caatccgcta tgttcagctt cgtcgtcaga
    40921 taatacttca acttttaacg gattgctgac ttcacggaca tatttcgtca atgcatctgc
    40981 gtctgcagga ggcatttgtc ccccatcttc tccgtagact ttatatccgt tgtaagctgc
    41041 tggattgtgg gatgcagtta ccatgattcc cgcaaacgtc ttcaaatgac ggactgcaaa
    41101 agaaagctct ggtgttggac gtaaactttc gaatacataa gaagggatat tgtgatgtgc
    41161 taatgttttg gctgcttcca tggcaaattc tggtgattga tggcgtgagt cataagcgat
    41221 agcgacacct cggcgttttg tttcttcacc ttgtgtatcc atgaaacgtg caagtccttc
    41281 tgttgcttgt cttaccgtat agatgttcat tcgattgatc ccaggtccta agattccccg
    41341 catgcctgca gtaccgaatt ccaacggtgc ataaaaagca tcttcaagtt tttcaggatc
    41401 ttcacccaga tcaattaatt gtttttttaa gttttcttct aaattttctt catgtaccca
    41461 ttgttcatac acttgttccc aagacatgaa aataacacct ctttctgaaa tatggttctt
    41521 aataagtata gcattcactt attagatatg aaagtacgag gtctaaaaaa cgcttaaatc
    41581 aagaaaggat tcttttatca agcacggttg caagtattcg cataaatcgg attcagtaga
    41641 tttcacgcga aaaaaaagag taaaagaaca attgaaaccg ttcttccact ctttttaaac
    41701 ttatgcagtt gtttcttcta aagattccaa gtatttatac acttgttgtc cagcgatccc
    41761 accatcacca acagcagtag tgatttgtcg caaatctttt tcacgaacat ctccgatggc
    41821 aaaaacgcct ggtatattcg tcttcatttc gttgtctgtt ggaatccagc cagcttcgtt
    41881 cgtcaaccct gcttctctaa atggtgcagt taatggatcc agtccaacat agatgaatac
    41941 tccgtctgct gccaaatcaa actcattgcc ggttttgaca ttacgtgctt tcacacctgt
    42001 cactaccatt tcattcccaa cgatttcgtc aacgactgta tcccacataa aggaaatttt
    42061 ttcattcgca aatgcacggt cttggatgat cttttgtgca cgtaattcat ctctacggtg
    42121 gacgatgact acttcagaag caaattgtgt caaatagatg gcttcttcta ctgctgagtc
    42181 tcctcctccg atcactacta gacgtttgtt acggaaaaac gcaccatcac aaactgcaca
    42241 gtaagaaacg ccacgtccag caaattcttc ctcgccaggt acgcctaatt tacggtggat
    42301 acagcctgtt gcgataatga ctgtttttcc ttcgtaagtt ttatcttcac aaataacttc
    42361 tttgtggctg ccgtgatcct tgatatcttg cacgattcca taagcatttt ctgtcccaaa
    42421 tcttgaaaca ccttcataca tcttataagc tagatcagga cccatgattg aatcgaaacc
    42481 aggatagttt tcgatttctg ccgtattgtt catttggccg ccaggggcgc cttgttcgat
    42541 catcaatacg gataagtttg aacgtgaagc atataaagca gcagtcatcc cagcaggacc
    42601 tgcgccaata acgattacat catacatatg tagattctcc tttacttaat caagtaagca
    42661 tactttacag ccaatgcgaa aaaaagtcta ttgttctgct tgcgtttctc gaatagcttt
    42721 attatactcg aaaaatccgc tgaaaaacaa tagactttta caaatcattg atcaatcatt
    42781 cgaaagattt tctccatttg aatcaattac ttttttatac cagttgaatg atttcttctt
    42841 cgaacgggct aatgtgccat ttccttcgtt gtcacgatcg acataaatga atccgtacct
    42901 tttcttcatc tcacctgtac cagctgatac taaatcaata catccccaag tcgtatagcc
    42961 taatagatcg actccatcta attctactgt atctttcatt gcctggatat gtgctgccag
    43021 atagtcgatt cgatagtcat cttccacata accattctca tcgggcgtat cgactgcacc
    43081 taagccgttt tctacaataa atagtggttt ttgataacga tcataaatat cattcatcgt
    43141 tattctcaag cctaatggat caatttgcca accccattca cttgcttcca gatatggatt
    43201 tttgacagaa gcaaagatat ttcctgcagt ctgttccaag agttcgttgt cagtagtcga
    43261 aactcgagaa gaataataag aaaaggagat aaaatcaaca gtatgttctt tcagcaatgc
    43321 ggcatcccca ggttccattt tgatctgaat gccatttcgt tccatttctt tcaatgcata
    43381 agcgggatat tctcctcgtg actggacatc aatgaagaaa taattttctc gatctgattt
    43441 tctagaagcc caaacatctt caggattgca agtgtatgga taatatgctc ctgctgccag
    43501 catacagcct actttgttct ccggatctac ttcatgagca atcttcgttg cgatggcact
    43561 tgcaaccaac tcatgatgtg ccgcttgata tttgatttgt tcttcatttt ctccttcttc
    43621 aaaatacagc cctgcgccca tgaatggtgc atgaaggatc atattgatct cattgaatgt
    43681 caaccaatat ttgacaagtc ctttatagcg attgaagatg actcggcaca gattttcata
    43741 aaaaccaacg agttctctag aacgccacgc accatattct ttgatcaaat gcatcggaca
    43801 atcaaaatgt gtaatcgtca caagcggctc gatcccgtac ttttgacatt ctttaaagac
    43861 atcctcataa aatttcaaac cagattcatt tggctcattt tcatcgccca ttggaaaaat
    43921 acgactccaa gcaatggaca tccgataagt cttgaacccc atttctgcaa acaaagcaat
    43981 atcttcctta taatgatgat acatatcgat cgcaattttt gctggataaa aatgatcttt
    44041 atcaaattca aacattttct tttttccagt aatgacagga aaacggtctt ctccgattgg
    44101 cacaacatct acgttcgcta acccacgtcc gccttcatca taaccaccct cgcattgatt
    44161 ggctgctgta gcaccgcccc ataaaaaatc ttttctgaat cccatcatac ttcctccatt
    44221 acatgtgtat tgcttgacaa ttctcttgcc agttcatctt ggacttcgat tccttctgct
    44281 tttaaccggc tgattgtttc cttgaattgc ggtaaatact gctgatgtgc aataaacaac
    44341 tcatccatga tccgttttgc tgtctttcct gatcgtataa gcggattgat cgtaaacgct
    44401 tgtaaagctg ttccataatc accagtcacg gctgcttcga tcgtcagcaa ttccatattc
    44461 ttcattactt gcaaccagcc tctttcagca gcaggaaggg aaccaaacgc tacatttcgt
    44521 gcgccttgtg cgccaacata ggcagtgact tctactacac aatctgccgg cagatcagga
    44581 acagctccat cattctttgt agacaccacg atctgtgttt ctttatttcc atagatcgaa
    44641 gcaatcgttt cacaggcagc atctgaataa taagcaccac ctcgtttttg caattgttca
    44701 ggtttatgat ccaattctgg atctttgtat aattcaaaca gctctgcttc tgtttctttc
    44761 acttgttgcg ctcttgtacc aatcgtttga tattcttcta aagcatgttc caacatttct
    44821 tcttcacggt agtaatagcg gtggtagcca caagggatca tgttcatttg ttttaattgt
    44881 tctttgaaaa acgggatgtc aaagatattt ttcggcaatc cattgtcttc atcgtataaa
    44941 cgatcaatca aatctgctgt gcggtcattt cctttattat ctgctacttt gtgccaatgg
    45001 aagtgattta gaccggcaaa cttgtagatc agctcttctt ttttcttgcc aagcattgct
    45061 ggttctgtca tcgtcgccat tactggcaca ttgcataatc cgatcacttt atcccatttg
    45121 ccatatctga taactgcttc tgtcaccatc ccgcttgggt tcgcgaaatt gatcagccaa
    45181 gcatttggac ataaacgctt catgtcttca acgattgcca aaaccactgg aacggtacga
    45241 aaagctttga atatcccgcc agcaccattt gtttcttgcc caatcatccc ataataacta
    45301 gggatccgct cgtctttgat tcgtgcatct aattgaccga cacgaaactg agtcgtaaca
    45361 aaatccgcat ctttcaatgc ttcttcccga tccaaagttg catagatctt gacttcatac
    45421 ggagaagcat cccacatacg ctgagacatc tgactgacga tctcaagctt ttcttttcct
    45481 tcttctacat ctacgagcca gatttctcgt atcggtaatt cctcatatct tttgataaat
    45541 ccttccatca actcgggggt gtaactgctg cctccaccaa tcgtagcgat tttgattcct
    45601 tttgccatgt tctcactcct ttgcttttta cacttttatt atcttttttg taaacaaaac
    45661 aaacaataga aattcgtttc tttccagtaa catttacaaa agttagtgtt ttctactgga
    45721 tttttgtaaa tatcttgtca aaaaatgact ttattaagct atataacata aatccgttta
    45781 caaatcattt tcaaaaaagt tataatttac gtaaaaaaac tagaagaagg tatctacatg
    45841 ttattacgtg aaaaaatcaa agaaatctcc ttttctcctt cagaaaatga agtgattcag
    45901 tttctcttaa gcgattctcc agcacttggc gaaatgacca tccaagagat tgctgagcga
    45961 tgttatgtcc acccgtccac actcatccgt gtagcaaaaa aaatgggttt tgatggctgg
    46021 gtcagttttc gtaatgcttt tttagctgaa acagcctatc ttgaagggaa ttttcaagac
    46081 gtagatgcga atcttccttt ttccaccaca gacggtatta tgacgatcgc aaataaaatg
    46141 gcttctttag aacagatgac gattcaagat acactttctc tcattcatca tgaccaactg
    46201 caaaaagcca aacaactaat gcttcaagca aaaaaaatcg tgattttcgc cagtaactcg
    46261 aatactttga tttctcaaga tttcatgctg aaaatgaagc gcatcaaaat ggatgtcacg
    46321 attgtcacca cttttggtga atcgttctat gaagcgtaca attgcacgag tgatatgtgc
    46381 gggattctga tttcttatac gggtgaaaac aatgtgatcc ttcgtaccgc agaaatattg
    46441 aaacaaaatc atgttccaat tttagcgttg accagtatcg gtgattcaac gctatcctca
    46501 atgagtacag ccgctttgcg acttacgaca cgcgaacgac tgtattcaaa aattggtaat
    46561 tttacgatca atacatcgat ttgttatctt ctagacgtgt tatatagttg tgtttttgca
    46621 gaaaattatc aacagaattt agtccatcta atttctgtag gacaaaaatt tgataaacgc
    46681 cctatcagct cagccatcat gaaagagcct ttcaattttg aaaataaaga agtggactaa
    46741 aaagaatagt taagtctatt ccattctttc acctaaccgt tcaggtcagt gcggtgataa
    46801 gtgcatcgtc tggcccgacc gtctcccctt gattttccaa aatatccaaa taatccatgt
    46861 aattggtgac gattacaggt gtttctaaac tgtatccttc cttttcgatt ttctttttgt
    46921 cgaagcggag caacagatcg ccttgtttca ctctttctcc ttgctgcacc aagctttcaa
    46981 aatatttccc ttccaactga acagtatcca atccgacatg aatcagcaat tccagacctg
    47041 tttcagaaac cattccgatt gcatgtttcg tggggaacag ggtcatgact gttccgtcaa
    47101 aaggggcacg tacttctcct ttttcagggt agatcaagat tcctcgacct agtgttcctt
    47161 gtgcaaaagc tgcatccttt gctgctttca gtggagcgat ttgtccttgt atcggtgttt
    47221 ttacaatttc tttacctgat cttttctgtc tgtcatctgt ttcacctgct tcctccttcc
    47281 aaaagaaaaa agtcagcgca aaggatacca ccatcgcaat ggcgatccca atcaatacat
    47341 taatcacatc tgttaaatca ccatctggtc taatatagtt gggaattgta aagatgccta
    47401 gtccaccttg attccaagaa gttaaattca ttaacccact atacgcgccc cctaacgcac
    47461 caccgacaca agtaaaaata aatatttttt tacgtggtaa tgtaaaccca taaatagctg
    47521 gctctgtaat ccccacaata ccagaaacaa tggctggtat tgcaattgct ttgtcttttg
    47581 gatttctaag cttaaagtag agtgcggtaa gtgctgcagt ttgtccaaaa gacactactg
    47641 caacaggtgt cataatattt tgccaaccat tcacagaaaa ttgcatcaat cccattggta
    47701 cgattgccca atggagacca aacatcacca gaatttgcca gaagaagcct acgattgctc
    47761 cataaataac tggcgaaagt gctaaaagtg tttcaaatcc agccattaaa atatcagttg
    47821 cgaatgtgaa tactggtcca acgagtaaaa aacctaaagg cattgtaata agaacggtga
    47881 agaatggaac gaagaaattc gcgatcatct caggtactaa ctttttaatt ggtttctgaa
    47941 tttggctagc taacaacaca ataaagataa tgggaatgac agaagaagcg tagttacgcg
    48001 caatccaggg aatgccgaat actgtttgat aaataggtgc ttcaaaaata gtgccagaaa
    48061 atatggtcgt tagtggtgct tctgctgcac cactcaaagc ttccagcgac aagctaggat
    48121 tcattaatgc tgcgccaagc aacatcccta cgaattcatt tactttaaat ttacgggcag
    48181 ctgttaaacc gataataaca ggcataaatt ggaatacaga atcaccgata ttatcaaaca
    48241 taacaaatgt gccagaaccg cggtcaaacg cgcctaagaa gacaaggaat gctgtcagtc
    48301 cacgaatcat cccgccagca gctaagatag caaggaatgg ttggaaacag cccgatattc
    48361 catcaataag catatcaaac ggtctcattt tttctgttga aacagcctca tcaaaagtaa
    48421 ggtttcctgc ttttataacc tcttcaaaca cgactggcac atgattcccg atgacaactt
    48481 gatactgccc tcccgctcgc attaccgtta caataccgtc catatttttc aagatctcat
    48541 catttgcttt ttcttcgttt tttaatttga aacgaagcct tgtgatacaa tgtgtcaaac
    48601 cattgatatt ttccttccca ccgacatttt ctacgatttt tctcgctaat tcttggtatt
    48661 ttcccatttc tattccctcc aatatattag tggaaggttt agccaactga atgtcactac
    48721 ccttatttca tacgttcttg ttaatttatc ctcctttttt tatttattta caacgattct
    48781 catgcactaa acgcgcagta tggatcgtta aatacatttt ttcgtccgtg gacatctggt
    48841 agttgaacgt ctcggctaaa aagtctgcta ttttctcaac gcaaccaaac gtatccttat
    48901 attttttttg aataacctga aacaattcct cagaaacttc tcccacttct gatttttctc
    48961 catctaggac gcgtgtcaga aaaaaacgta agtgtgtgac gaatcgaaaa aaatggactg
    49021 aatcttcatc aaatgtaatc ttatagtaat agcggacgat atttagaata tcctgtattg
    49081 ttatagtaaa caggtaggta ttgttttgtt ggctttgaat gccttgtgca ttgactaaat
    49141 gcaatgcgat aaatccggct tcatcttctg ataacttgat gtggtattta tccgagattt
    49201 tcttcactgt ttttttcccg atggcatact ctttaggaaa tagttgtttg atatcataca
    49261 gcaatacatt tggtacaaat attcccattt tgtgacgttc gatagccgta tacaaatgat
    49321 ccgtcaatgc aatatacaca gaatcattca actggacttg tagagcctct tttgtatatt
    49381 caatgatttc acaagaaatt tctaagtact caagaggaat cgcttgtgcc aattcttgga
    49441 actgttgtga aagcccgtca tttgtcaact gaaatttttt gtcgatgcat gagtcaaaaa
    49501 tcgggtcacc cactttcttt ttgaatgcca aaccttttcc catcacgatc atttcctgat
    49561 ttctttcatc cctggttatt acgacgttgt tattcagtac tttctcaatc agcatgtttt
    49621 gctcctttcc atgatatata gggctgtatt caaaacaaaa agaaaaaccc aaaaagccgt
    49681 ccatggaacc atgggagact catcaggttt agcttgcatc aggcaatcac tatccaagtt
    49741 gtatcgtttt ctttatatta tcgaaagcaa acgcttttgt caacgctttc ttagtggcta
    49801 ttaggcactt ttgactagct gtcgtcgtct tttttaaatc gggtgactac gccactaacg
    49861 ctcacagatg aagaatctgg gaagctctgt tttattttca ctctagcgct taggacaaaa
    49921 tgctactatg ctactgtcac aacctctttt tacacaccac tgctttgcag tttgtacata
    49981 tctgcataca aaccattttg cgctagaagt gtttcatgat tcccacgctc tactatatga
    50041 cctttgtcta aaactaaaat caattctgca tcccgaatcg tggacagccg atgggcaatc
    50101 gcaatcgttg tccgtccttg tcgcattttt gccaatcctt cttggatcaa tccttcggtt
    50161 tcagtatcga tatttgctgt tgcttcatcc aatactagta ttttaggatc tgtcacgatt
    50221 gttcgcgcaa aagtgatcag ctgtcgttgt ccactagaat agctggcacc gtgttcgatc
    50281 acttttgagt gatatccttt tggtaattca tcgatgaact tgtttgcttg aacaaactca
    50341 gctgcatggc gaatctgttc gtctgtgata gctggattca tcatccggat atttcctgca
    50401 atatctccat aaaacaagaa ggcatcttgc aagaccaatc ccattttctt acgtaactct
    50461 tccattggat attctcgaat gtcatgatca tcaatcaaga tttggccttc ataaaattcg
    50521 taaaaccgca tcaatacgtt gatgatcgag ctttttccgc ttccggtgtg ccccactaat
    50581 gcaacggtct cacccggatt agcgacaaaa gagatattgt tcaatacatt gtgctctcca
    50641 tcataagaaa agcttacatt gcgaaactcg atcttacctg aagtaatcgc ttgatctgct
    50701 ccaggatttt gttgcggtgc atattcttct gtatccatga ttttcaaaat acgactaccg
    50761 gcaaccatcc catctgtaaa ggtgctcaaa aagtccatca tttgagacat tggattaaaa
    50821 aatccctgga cataggtcgt aaatgcatag atcagtccaa cttctacggg agaatgcaac
    50881 gcatcgatcc caaacaaagt caacgccaac gcaatcgcca atgcatagag aaaattgatg
    50941 atcggactta acagaatcga attcgttttg atcatcgcat agcgcgtgtc taaatactcg
    51001 ttgttcactt gttcaaattc tttagctaaa cgagcttctt ggcgaaactg ctggataata
    51061 cgcatgccgg aaatcgattc attcaatttc gtgttcagct gacttaattt ttcccgcata
    51121 tttcggtaaa tacgagaact gaatttctgg taataccaga tgacgattcc taaaatcggc
    51181 aagaacacca aagtccacaa ggcaatttct ccattgattt gaaacatggc aacgaaagaa
    51241 gaaacgactg caaagatccc agtcaatacc atcaaaaaga cataccaaaa ttcaaatagc
    51301 gtttctgtgt cattcgtcac tcgagaaacg gtcgatcctg ccggtgtctg gtcaaaataa
    51361 cgcataccta gtgtatgcag cttttcaaat aatcttaccc gaacatactg ataggtcttc
    51421 aatgaagcca tggaatacaa ataccattgg aaaaaccaaa tgatactttt gactatgact
    51481 ccaaacaagt acacaccagc aaaaaacaaa ataacttgtg tcgtagctga ctgtttagcc
    51541 agatgatcat ccataaaagt ttggatgatc cttggcaata ggacattgat cacagacagg
    51601 gcaaacgcaa ataagatagc aacgatgaac gttcttctaa aaggtttggc gaacttcatc
    51661 agtcgtttga tgatcttctt ctgttcttta agtggaatcg attttgtcca ttccgattga
    51721 taattttcat ccatcaggcc tcgcctcctt ctatcttcgc ttctaattgc tgtttttccc
    51781 acattcgttc ataccagcca cctaatgcaa gcagctcgtg gtgggttcct cgttcaatga
    51841 ttttcccttg atcgacaaca atgatttctt ttgcatgcat cacgctgctt aaacggtgag
    51901 ctgcaatgat ggttgttttc tcttttctta tttcttttaa attatgcaag atagcttctt
    51961 ctgttttggc atctactgca gaaagtgcat catctaagat cagcaattca ggatttttga
    52021 tcaatgctcg agcaatcgaa atcctttgtt tctgtccgcc agataaagag actccacgtt
    52081 ctccaactaa ggtatcataa ccttttggca attccttgat ttcattgttg ataaatgcta
    52141 aagaagccgc atgctctact tcttcttctg aaagttttgg attagaaaaa cggatattat
    52201 ctcgaatggt catcgaaaac aggaagtgat cctgaggcac ataaccgatt gatcctaata
    52261 aagcattcaa tgaatattct ttgatcgaac gtccgccaaa ttggattttc ccttgataat
    52321 catcgtattc acgcatcagt aatttgatga ttgtcgtttt tcccgcacca gttttcccaa
    52381 caatacctaa tgtttctcct tcttggatcg caaatttgat ttgttctaat gctggtgatt
    52441 cttctttcgg atagttaaaa gaggaaatag ctacattcag ctcgcctttt gccggggtct
    52501 gcactgcacc cagttgttcc acgatatgcg tcttttcatg caaaagttca ttaacgcgat
    52561 cataagaagc atttcctctt tccaatacgt tgaacaatcg gccaatcgca aacatcggcc
    52621 aaacaagcat accgatataa ctaataaatg agaccatctg cccaatcgag atcgtgcctt
    52681 ccaagacata ctttccacct aaaataattg ttgcaacata agacagaccg ataatcaagg
    52741 taatgaatgg atcaaataag gagtctaaaa aattgactcg tttgttttta ataattgcat
    52801 cttcaatctt ttcttcaaaa tccgcgagat cttctttttc ttgtccaaat gttttaatga
    52861 ctttgatccc agtgatactt tcttgtgtct tatcattgat actagaaaaa gcttcttgcg
    52921 aatcacgaaa agcatcgtgg agttttgacc ccagcactcg tgaagtaacg gctagcaaag
    52981 gcagaggaat cagcgcaatc agtgttaagc gccaatcaat aaacaaaatc atcgcaatga
    53041 tcgttgttcc gcccgtaatc actgaatctg caaaggtcaa gattccagct cctgctacat
    53101 tctggattgc attcaaatca ttcgttgcat gagccatcaa atctccagtc cgatattttt
    53161 ggtaaaagag attgtccatt tctgtaaaat gtttgaatag ctgctgccga agttctttct
    53221 ccaaccgagc tgcacttccc caaatattcg tccgccagat ataacgaaaa agatattgta
    53281 agaaagctgc aaggatcaat agtaagatcc accaagaaat gatctttatt gataaattct
    53341 tatcggcgat ctcatcaatg atgatcccaa tcacttttgg aggaactaac tgtaccaagg
    53401 ccactgcgaa tagggagaaa atcccaatta aataatgttt cttttcctga cgaaaaaacc
    53461 aacctaattt tctaaaaatc gacattttac tcctcctttg tctttatcaa gagcatagga
    53521 ttgtctgtcc ttgtcttttt tgctgaatag tctgaatagt ctaacaaatt acgaaataaa
    53581 aaacggactg aaaaaatcag cccgcaaaaa gtctttgggt cagaacctat tttttccctt
    53641 ggttcttcat ttgctgcatc atctggcgaa ttttcttctc agatggtttt tgccccattg
    53701 acatcatcat tgatcgcaac atgtcctcat taacgggagg atttttttct aaatagtctt
    53761 tcatatattt acgcgctagg aaaaagccac ctactgcgcc gattagcaaa gcaataatag
    53821 caataagtac gacaatacct gttgatacca tataaatctc tcctttctat ttccgaattg
    53881 ctctcaatga tttattttac taaagtttat tgaaaaaaga aaggcgtatt ctttagtttc
    53941 atgcaaaaaa tagtggtttt tttagagaag aacaaaattt ctttcattaa ccagcaagac
    54001 acgccacgaa aaaaaattag gcataccgaa tctttttctt taattttttc tttctgaggt
    54061 gtagaatgta atcgtagata aataggaaag gtggtttttt cttgaataat caatccaaca
    54121 aacaatttca taagcatcga cttattgaac atacaaatgg tttatcttta gctgaagtaa
    54181 atggaacagt ggatgtacca aagggaaaaa gtttttttaa aacacttctt gcttactctg
    54241 gaccaggtgc attagtagca gttggttata tggatccagg taactggtcc acttcgatca
    54301 ctggtggaca aaatttccaa tatttattga tgtcagttat tttgatgtct agtctaattg
    54361 ctatgctttt acagtatatg gcagcaaaat taggaatcgt gacccaaatg gatttggccc
    54421 aagcaatccg agcacggaca agtaagacat tgggtgtcat tttatggatc ttgactgaat
    54481 tagccattat ggcaacagac atcgcagaag tcattggtgc agcaatcgcc ctctacttac
    54541 tttttgatat tccattggta attgccgtat ttatcactgt gttagatgtt ttcgtccttt
    54601 tattgctgac aaaagtcggg tttcgtaaaa tcgaagcttt ggttgtctgc ttgattttcg
    54661 tcattttatt cgttttcgtc tatcaagttg gcttgtctaa ccctgattgg cacagtttat
    54721 ttgctggctt tatccctaac agtcatacat tctcagactc acctgtcata ggtggtgaaa
    54781 ctccgctgac tggagcgtta gggattattg gtgcaacagt catgccgcat aacttatatc
    54841 ttcattcagc agtttctcaa actcggaaga tcgatcacca agacgaagaa gatgtagcac
    54901 aagcggtacg cttttctact tgggactcta atatccagtt gtcaatggct tttttagtca
    54961 atgcactgct attgatcatg ggtgtcgctg tattcaaaac cggttctgtc caagatcctt
    55021 ccttttttgg tctgtatgaa gctttatctg atccatcagc tatgagcaat ggtatcttga
    55081 ttgctgtagc aaaatctggt atcttgtcta ctttatttgc tgtagcactt cttgcttctg
    55141 gccagaactc tactattact ggtacgttga ccggtcaagt catcatggaa ggattcatcc
    55201 atatgcgtat cccgatttgg ctaagaagac tagtcacacg attgctttct gtgattcctg
    55261 tattgatttg tgttttgttt acaagtacaa aaggaacgat cgaagaacat accgctttga
    55321 ataacttgat gaatgaatcc caagtttttc tggcttttgc attgccattc tcgatgattc
    55381 ctttactgat gatgaccaat agccaagcgg agatggggag ccgattcaaa aattcatttg
    55441 ttgtaaaact tcttggctgg ttctctgtga ttagtctgac ttacttgaac ttacgaggat
    55501 taccaggtca aatcgaaagt ttcttcggcg agcaagccac ctcagctcaa ctagcattgg
    55561 ctgatcaaat cgcctatgta atcattgttc ttgtccttct cctgttggca tggacgattg
    55621 gtgatttata taaaggaaac aaaaaatatg aacagcgttt gcaagaaacg atagaagaaa
    55681 tcgaatagaa actgattctt ttacacaaag gaggttgtga cagaagtggg cagcttcaag
    55741 aaataagaag gaatttaaga aaattgcttt tcaaattttt gtgaaatttc ggcttatttc
    55801 cgaagaagtt acttctggaa caccgtgtat
//
LOCUS       DACGLW010000008        50485 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.008, whole genome shotgun sequence.
ACCESSION   DACGLW010000008 DACGLW010000000
VERSION     DACGLW010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 50485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.008"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            complement(183..896)
                     /locus_tag="IXT53_000563"
     CDS             complement(183..896)
                     /locus_tag="IXT53_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAQ4904417.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            complement(964..1290)
                     /locus_tag="IXT53_000564"
     CDS             complement(964..1290)
                     /locus_tag="IXT53_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAQ4904418.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            complement(1413..1640)
                     /locus_tag="IXT53_000565"
     CDS             complement(1413..1640)
                     /locus_tag="IXT53_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HAQ4904419.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            complement(1962..3215)
                     /locus_tag="IXT53_000566"
     CDS             complement(1962..3215)
                     /locus_tag="IXT53_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ4904420.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            3276..4016
                     /locus_tag="IXT53_000567"
     CDS             3276..4016
                     /locus_tag="IXT53_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ4904421.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            complement(4102..4860)
                     /locus_tag="IXT53_000568"
     CDS             complement(4102..4860)
                     /locus_tag="IXT53_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAQ4904422.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(4857..5885)
                     /locus_tag="IXT53_000569"
                     /pseudo
     CDS             complement(4857..5885)
                     /locus_tag="IXT53_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
     gene            complement(5905..9072)
                     /locus_tag="IXT53_000570"
     CDS             complement(5905..9072)
                     /locus_tag="IXT53_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4904423.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            complement(9296..10180)
                     /locus_tag="IXT53_000571"
     CDS             complement(9296..10180)
                     /locus_tag="IXT53_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAQ4904424.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(10275..10712)
                     /locus_tag="IXT53_000572"
     CDS             complement(10275..10712)
                     /locus_tag="IXT53_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4904425.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            complement(10929..11840)
                     /locus_tag="IXT53_000573"
     CDS             complement(10929..11840)
                     /locus_tag="IXT53_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ4904426.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            12270..13460
                     /locus_tag="IXT53_000574"
     CDS             12270..13460
                     /locus_tag="IXT53_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HAQ4904427.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQ
                     LIEN"
     gene            complement(13641..14069)
                     /locus_tag="IXT53_000575"
     CDS             complement(13641..14069)
                     /locus_tag="IXT53_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4904428.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            complement(14097..14735)
                     /locus_tag="IXT53_000576"
     CDS             complement(14097..14735)
                     /locus_tag="IXT53_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4904429.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(14739..16775)
                     /locus_tag="IXT53_000577"
     CDS             complement(14739..16775)
                     /locus_tag="IXT53_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HAQ4904430.1"
                     /translation="MIIKKKFTVLLTIIVTGIMSGFALVSINKQVLYRTNEFFHQTDT
                     YTPIRLPQHFIGQEEYEQLIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLE
                     MTLYYQGDVKSSHEKVPYSNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERVISLF
                     TDRFNQAFHTDYATNIFEDFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLL
                     MNKEISLLLNNDFSLHQVLWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYV
                     FLFSGIYGISLISLLLVEKIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFS
                     SSQLAVSHEPPKEIAGEDYHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSI
                     LVNVSDYLNEENEQFGRGIKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRH
                     TDLKNDLAQITEYYQEISEKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSM
                     YLERNILSGEIYPPLKIKIGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLS
                     RSFHYLIQIFLLTFFSFFLLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNL
                     IKWGTTSVIFLFLIEREPKYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            16901..17566
                     /locus_tag="IXT53_000578"
     CDS             16901..17566
                     /locus_tag="IXT53_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ4904431.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDF
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            complement(19501..22650)
                     /locus_tag="IXT53_000579"
     CDS             complement(19501..22650)
                     /locus_tag="IXT53_000579"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HAQ4904432.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSSEQINRMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDN
                     AYTKFDFRNGRPTKSAILTTSSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTM
                     EDPDFPRIAITYSLEENSRDSSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNR
                     LARKRAEFKQFGKQIDLAIVVDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTY
                     PDKTKGLIVTFRKPATMEKNVEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKE
                     VSSMESIDEHTPLEIRIEYVKAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEE
                     QIGVYETVKGSLIEEEPEKEGEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSD
                     AREEIEKALAKLNKTEGVKEVYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHV
                     FSNEWFVSEKELHASNLQYLPGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMK
                     RAWRKMLDDELIPLENELR"
     gene            complement(22663..23862)
                     /locus_tag="IXT53_000580"
     CDS             complement(22663..23862)
                     /locus_tag="IXT53_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HAQ4904433.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLIDLVVRLNKSTNSSRLPKVEF
                     EDIVAGEGRLNKDVSQKFDNRKGIEFLPNDILYGKLRPYLKNWLKATFTGVAIGDFWV
                     FRAKKSDSDFIYSLIQADRYQKAANDTSGTKMPRSDWKKVSDTVFYIPNDLKEQQKIG
                     IFFKKLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWEERKLGE
                     LGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIERKKWKPI
                     KDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFETINLPR
                     YSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLLKETKKS
                     FLQKMFV"
     gene            complement(23852..25447)
                     /locus_tag="IXT53_000581"
     CDS             complement(23852..25447)
                     /locus_tag="IXT53_000581"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HAQ4904434.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAIAPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEMWLQGALEVFKHEQ"
     gene            complement(26031..26738)
                     /locus_tag="IXT53_000582"
     CDS             complement(26031..26738)
                     /locus_tag="IXT53_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4904435.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENG"
     gene            complement(26803..27639)
                     /locus_tag="IXT53_000583"
     CDS             complement(26803..27639)
                     /locus_tag="IXT53_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ4904436.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(27657..29066)
                     /locus_tag="IXT53_000584"
     CDS             complement(27657..29066)
                     /locus_tag="IXT53_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ4904437.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(29080..30903)
                     /locus_tag="IXT53_000585"
     CDS             complement(29080..30903)
                     /locus_tag="IXT53_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ4904438.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(31746..32072)
                     /locus_tag="IXT53_000586"
     CDS             complement(31746..32072)
                     /locus_tag="IXT53_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4904439.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(32118..32480)
                     /locus_tag="IXT53_000587"
     CDS             complement(32118..32480)
                     /locus_tag="IXT53_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HAQ4904440.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(32517..32732)
                     /locus_tag="IXT53_000588"
     CDS             complement(32517..32732)
                     /locus_tag="IXT53_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ4904441.1"
                     /translation="MKKVNNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            32859..33194
                     /locus_tag="IXT53_000589"
     CDS             32859..33194
                     /locus_tag="IXT53_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ4904442.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(33270..35267)
                     /locus_tag="IXT53_000590"
     CDS             complement(33270..35267)
                     /locus_tag="IXT53_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4904443.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(35365..35640)
                     /locus_tag="IXT53_000591"
     CDS             complement(35365..35640)
                     /locus_tag="IXT53_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ4904444.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            35872..36606
                     /locus_tag="IXT53_000592"
     CDS             35872..36606
                     /locus_tag="IXT53_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ4904445.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            36645..37787
                     /locus_tag="IXT53_000593"
     CDS             36645..37787
                     /locus_tag="IXT53_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ4904446.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(37882..39273)
                     /gene="sufB"
                     /locus_tag="IXT53_000594"
     CDS             complement(37882..39273)
                     /gene="sufB"
                     /locus_tag="IXT53_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HAQ4904447.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(39278..39748)
                     /locus_tag="IXT53_000595"
     CDS             complement(39278..39748)
                     /locus_tag="IXT53_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HAQ4904448.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(39735..40970)
                     /locus_tag="IXT53_000596"
     CDS             complement(39735..40970)
                     /locus_tag="IXT53_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ4904449.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(40967..42253)
                     /gene="sufD"
                     /locus_tag="IXT53_000597"
     CDS             complement(40967..42253)
                     /gene="sufD"
                     /locus_tag="IXT53_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HAQ4904450.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(42266..43039)
                     /gene="sufC"
                     /locus_tag="IXT53_000598"
     CDS             complement(42266..43039)
                     /gene="sufC"
                     /locus_tag="IXT53_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HAQ4904451.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(43236..44084)
                     /locus_tag="IXT53_000599"
     CDS             complement(43236..44084)
                     /locus_tag="IXT53_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ4904452.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(44108..44794)
                     /locus_tag="IXT53_000600"
     CDS             complement(44108..44794)
                     /locus_tag="IXT53_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ4904453.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(44787..45821)
                     /locus_tag="IXT53_000601"
     CDS             complement(44787..45821)
                     /locus_tag="IXT53_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ4904454.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            complement(46216..46554)
                     /locus_tag="IXT53_000602"
     CDS             complement(46216..46554)
                     /locus_tag="IXT53_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HAQ4904455.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(46556..46912)
                     /locus_tag="IXT53_000603"
     CDS             complement(46556..46912)
                     /locus_tag="IXT53_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HAQ4904456.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(46918..48105)
                     /locus_tag="IXT53_000604"
     CDS             complement(46918..48105)
                     /locus_tag="IXT53_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ4904457.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(48296..49141)
                     /locus_tag="IXT53_000605"
     CDS             complement(48296..49141)
                     /locus_tag="IXT53_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ4904458.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            49375..50340
                     /locus_tag="IXT53_000606"
     CDS             49375..50340
                     /locus_tag="IXT53_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HAQ4904459.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
BASE COUNT        14795 a        10369 c         8441 g        16880 t
ORIGIN      
        1 ttttatttat gcaagccttt tcatataaaa aataaaaaaa ctggaacaca agtctatcta
       61 gaaaacaacc aaatcagaga tgcacctctt tgcgtttctc tttttttcag caaattggtt
      121 tttccttatc gattcatatc ctttggctgt ttttaatctg taacgactta tgtcccagta
      181 tcctaaaatg tttttgtctc tagattgaaa gcagatctca tatcatctgt cgcatctttt
      241 atcgatgtaa cactttccag ttctccttgt acaactagtc gcgtgacggc gtccatgtcg
      301 ccacaagtaa ttagtgtgac aatttctttt ccctcgactt cattcagtaa ttgggtatct
      361 gtaggagcaa ctttttcttt aaaaaatgtt ttgtatgtat acacattttc taaatctgtc
      421 aaatagatct tggcacccat ctctaaattt tctagcggtg taaacagcaa ttcagggttc
      481 gttgcacgat ggcttgctaa ggcataattt ccttttccca tttcttgatc tggagataat
      541 gttcctgctc cgtaaagtag tgacgtatta tccaagcctt tgaaaaccgg taaattgatt
      601 gaaactgaag ggactgcgat actagcaatc actggtagat ctgttcctct catttgcgac
      661 ctcatgaccc cttctgtcgt cattggttct accgcatcaa agtcaaaact gacatcttta
      721 tcgttattct ttttcagatc ttcttttgtt acattcgcta tagcatattt atctcctgtc
      781 tctctgacaa aataatcctt gatctgttca ttgaaaatca aagctagtcc tactaataac
      841 aagaggaaca agaggatatt gactagccat ctttttgctt ttcctttttt tttcattttc
      901 tctccctcca gactcatttt actgtttttc ttttgctctt tccaacagcc gaatgacaca
      961 tttctacaac tctgtgtatt tttttcgtac acctgcgctt ttttctacag gatatttcaa
     1021 attatttttg atcaattttt cttcaatgat ctcatctaga tccaggtgaa ggttgtctgc
     1081 catcatgtat gaatagatca atacatctgc cagttcttct ttgattcgtt ctaattgtgt
     1141 ttcttttact tcttccggcg ttttccattg aaaaagttct aacaattcag atgcttccaa
     1201 agaaatagag atagccaaat ctttttcgtt atgaaattgt cgccaattac gttcatctct
     1261 aaactggttg actttttcca tactattcat ttgcttgcct tcttccaatc acttcttact
     1321 tgattaatca taccattttt ttacataaaa aatagatgat gcttttaaaa aaactcctgt
     1381 tttcttttat ttaaaataaa tgtcatagaa aatcaataat ttaggtagcc accatgtgta
     1441 tgcagtagcg tgtcttcttc ccttgaggct ttttgttttt cgatacgttc ttcttcccaa
     1501 acagacaacg ctactagaat ctctcttaat gattttcctt cttcggtgag cgagtactct
     1561 actttaggcg ggacttggtt ataggctttt cgatggatac tttcatcttg ttctaactct
     1621 cttaactgct gggtcagcat tttttttgtg atcgtaggga tttgcttgct tctccacgcc
     1681 taaaataagc gatattgctc ttccctttat ctggtgtaag caaagcagtt agcattttga
     1741 cgatcgtggg ttccgctttt cacttcttat ctacttagta tcatgaagta tactttcgta
     1801 ctaatactaa gtagcggaag tgtcaactac tttgtttaac tacatagcaa aagaaacaga
     1861 agggtgacaa cggtcatcac gcttctgttt cttttgaata tgctgtgttg ttgtctctat
     1921 ctccgtgtag actttcctca aacttatcct ttgacagtaa gtcagtaggc tgatttgctc
     1981 tttctagatg ccagatacat ttctacaaaa aaatagtgat tctgttttga aaattcgatt
     2041 tttagttctg cattttcgta aacatccaac atttttttcg cactgtatag acctaacccg
     2101 ccattttttt tagaggtttt tcctcttttg accatctccg atagcgtcaa attcgttgga
     2161 tttgccttgt tcgctatact aaaacgtgat ccctcttttt cttccagcag attgatcgta
     2221 atcggactct gatcggctac acgatgatcg aatgcgttag aaataaaaat agataaacag
     2281 cgaataaaaa caaccagatc aatcgggatt gttttcactt cgacaggaat aactacttgg
     2341 atcgggattt tctccgattc tgctttttct agaaatttta aaaagacacc cctgacagct
     2401 ggggatacaa tatttgttag ctgttgtatt tgcggttttt caaaaatagc tgcagaatga
     2461 tcttctaata tttctaaaaa ttccaacgtt tcttttttat tttcctgaag agtaattttt
     2521 aatgaaaaca gtagactttt ataatcatgt ttgaattcct ctagatgatg gtaatattct
     2581 tcatttattt gatacagttg aagcgattcc tgatgttgtc tttcttttac ttggaaactg
     2641 atcaacataa tggaaaaaaa caaagaaaaa aaactgatga ctatcgacaa aagagataga
     2701 agcagataat tagatgagcg gaagtgaaca cttcccaata ccaagtaaat cacgccccag
     2761 cttaataaaa tacaaatacc tccaaaatag tgttcagttg gataagtaaa aagcaagttg
     2821 agaaatctcg tctgtttgtc tatacttttt actaatagat agaaaataaa tgcgataagt
     2881 agtgcaaaaa aacttgctgt tccaagatgt ctctttccta aataatccat cagatcaaaa
     2941 accagaaaaa agctgagtcc agtgatagca aaaaaaagat tactgatcaa acagctgata
     3001 aacaggtttt tcgtagtaaa ataaaaaaga aacatcaata ataagccaac taggtaagga
     3061 aagctcactt tagggatagg aagtaaaagt gtcaccacca tcaaagctat acaaccaaag
     3121 ctgtttttta ctagcagttg tttcgaaaag aataaataga tacctaaatc taaaatcaaa
     3181 gtaaaggtca aataaagtgt cactgttgcg accatcttat tcctccattg ttttgtttca
     3241 aaatgtacac atattttttt aataggagcc tgcatatgaa aaagtatttt atcatagaag
     3301 ataaccagtt tcacagcgaa tggatcatca gagaattggt caatcatcat atcgagaata
     3361 tcgtgcagct cacttctatc tctcaaatca atcagttgat ccagttgggc actattgata
     3421 atcacgatgt tttctttatc gatatcgact tgaagcaatc actcacaggt attgatgtcg
     3481 ccgaacaaat acgtaaaaaa aatgcacata gtaatattgt ttttttcacc gcttatacga
     3541 attttgcagt agagatcatc aatcggaaaa tccttccgat tggatatatc agaaaagaca
     3601 ggaatttatc tgaacaaatt cgtcaagtgc tagaacagat caattggaaa gaacagcaga
     3661 tcgtaaatgt cgataaaatc tttattgatc aaaaaggtgg aagtctcgtc ctgtatcctc
     3721 gggaaatttt atatattgaa agtatcaaag gtttgaaaaa ccaagttatg gtcaaaacaa
     3781 ctacagaaga taaattagtg aatacttctt taaaacaatt gaagacagag ttagaaaagt
     3841 atgcttactt tttactttta aaatcttact gtatcaactt atcgcaattg cagaaaattg
     3901 attctgctca ttatgtgtta gaatttttca acggtgactc cttactcgtt ggtcgaaaga
     3961 tctttgaaaa gacaaaggaa cgtttccatg acttccaaaa aactgcttcg agttgatgaa
     4021 taagagctga gacgtaagca tagcagattt cagggagtgg gccttgaatc ggctccactc
     4081 cctgaaatcg ttgatatgat tttacgcttc ctcttctgta ggagcatctg ttaacgacca
     4141 tgtgaatgtt gaaacgtagt tgtctacagc ataattggat acaggaacaa ataattccac
     4201 tgattgtctc ccttgttcca cattctctcc taatgaataa atccatgtgc caatcccttc
     4261 atttgctcct gcagttacta gatctactgc ctgatctgct gggatttcga ttccttcagc
     4321 gccagttcca gaaacggtag atggcattcc actgctatta gttgcgctat ttcgaatcac
     4381 ggcgtttttg aatttcacct ttgaaccagt tagtttattc gtatcagatg ttgtaccaat
     4441 aaattctgtt cgtttgactg ataatttcca accggcataa tttccgcttt tatccgttac
     4501 ttgtacgaaa ttctgtactt gtgtttgtgc tccagtactg tccgttccgc tttgtaatgc
     4561 agcatatact gtttttgctg tacctaactt gattgttcca aaatcaagat ctggagcttg
     4621 gtcaatcgtc agtaatccgg atgttccctg atttggttcg tcaggtgtaa aaggagtatc
     4681 aggatcattt ggatcaacag gaggaactgg aacaacgtct tcaggatttg cagcatctgg
     4741 tttggtcaca gaaatcgttc cttctgtagt ggctgagctc ttattctcag caaaaacact
     4801 ttgtgtacat aataatagac ttccagctag aacggtactt aatgcaattg tttttttcat
     4861 ttctttttcc ttctttcttt taaaataaat aatccagcaa ataagccaat aagaatgact
     4921 ccaacaagta tcagccatcg ccagtcaaat gtttctggct gattttttaa atcaatatcc
     4981 ttttcattca attggtccgc tgcatcatca gttatttcaa aatcggtatg gagattccag
     5041 cgtttttctt tccaagcacc cttcacaacc gcttgataat gtcccggagc taattttttt
     5101 ccttctaatg ggacaagtaa atcaaaattt gtattgggtg ctacatctat tttttctttt
     5161 tttattgaaa ggtaaggctc ttttgtttct tttttgtaga tttcaaccga tgtagtcaag
     5221 ccgaacatat aagaagcatt tggattttga agattcgcct taatcacatt tcttccatcg
     5281 ctttgatccg cataagcttt ttttaatttc aaatctgggg aagactcttt ttcgctattt
     5341 caaatcaaga ttgctttcac ttgagaaaac tgattgacga caccaactgt acttttgtta
     5401 gaaacgttct cttttttttc ttgaaacgtg atccctcctg cgaccacacc agccagctct
     5461 ttttctggcg ctttcagtga aagtgtctca gtgaccgcac tttgaggagg aacagttatt
     5521 tctcccactg tttttgctaa gtttttcata tcataactta atgtttcatc aaatttactt
     5581 tttgattctc catattcgat cacaccgttt gaattcgtcg tagcggagtg gatcaatgtc
     5641 tcaactgtta ctgatttatc tgttcggtta aagaccgtgt aggggatttc ctgtgttttc
     5701 ccaggctcca tccttacatt tatatatgtt ttgttcttat caatctgatt agccggtaat
     5761 tcaaaatcta ccgcaaaatt gaattcggag gcatggacag gtgtctgcca aaggatgaat
     5821 ccaaggagaa ggatcataag taaagagcag ttgtttcgat gacttttatt ccatattcga
     5881 ttcatcaatc atcctccttg atctttattc tgtcggcgca tctgctaatg tccatgttat
     5941 ctctgctgag taattaccag ctatctgatt ttctttggtt aagacaagtt taaacgcccg
     6001 accgttattt aaatattctg aataatctgc ttgcacattc gttgaatttt gtcctctgac
     6061 aatttccagt tcttcttcat tcactaattg atactcctct cctttcttga aatataggac
     6121 atcctgcata gaaaatcctg catcatttgt gaaaggctga gataatttta tcgttaagat
     6181 ccacggattt ttcgtttctc gctgatcata aatccataat ttttcctgta tatccatttg
     6241 gccaaatgct tcttgtgtca aaccagttgc tttgatctga ccaaaatcga gtttggaagg
     6301 ggcaagaaat gagagaccac cttctactac gatcttcgta gcaggtgcaa aaggcggggc
     6361 atcatgataa acaactggcg tatcacttgg attgtgattc ccaatttcat cgttggtact
     6421 tggcttgttc tgcacaccct tagcagtagc ttctcctgct tggtcgttta atacaatttc
     6481 taatacatct cctgcaaaaa gttgattttc tgggatagaa agaacaaatt cccctgtatc
     6541 atcaatagtt gtttctgaca aaacagcttg attttttttc aatgaaacca aggctcctgc
     6601 ttcacctgta cctgtgatcg tgcgagaagc tgcattgatc aatgtggtac ttaaattagc
     6661 aggttcagga ggaatcacat cgactgtcgt tttggcagag tccagttctg tcacttcttg
     6721 gccacctctt acttttttag cagcaacaac ttgcacttga tcctccgttt ttaatctctc
     6781 atcattgggt acttgaactt cgaacaagcc gccttgttcg ccttctcccc atgggttata
     6841 tcctgcccct tcattttcat ctgtttgtcc ttgcgtgaga gcagtaattt ctacagatga
     6901 tttcgtcgta tctgcgtaag taatacgtaa agtcacttct acttcgcctg tccaagcatc
     6961 tcttggcttt gcctcgacac cttcgggaac aacgacatgc ccatagattt ttttatctgc
     7021 attcgttggc actcttaagg attcaatcat cgggagctga ttattggcac tgattcgatt
     7081 ataatttttc attccggcac ctgtctgagc aaattcattt gttaaacctg cactagggct
     7141 tgatacgaaa ttgcctaagt cggtcccatt gatcgataag tctgtcatat cagaaaaata
     7201 atccgggtct ttttcaaagt tatcgaatgt tgcgcctgtt tttcgccata aagcgatttc
     7261 tgaatgggcg atctttaaag aagaatctcc atttccttgg aatacccagc cgcctatgcc
     7321 attggtctcg cttccaatcc gtttgttttg gaaatcaaaa taggtaggag attctaagtc
     7381 cacattcaac acatccccac tgatgatgcc ggtcttatct tcccctgttt tcccgaccat
     7441 gactaaataa gttttttcac ctgccgtaaa atttaaatca atatctccag ttgtatctac
     7501 agttgctccg gaatctgcgc gtatatcgat atttgatttt tctccagtga ctgtaaaatc
     7561 agctgaacct ggactttcta gaaaaccaga agcgtcataa aattggatcg cttgatttcc
     7621 ttgattgagt ctgttcacgt aagatttgcc atcacctgta tttcttaccc tcaatgagcc
     7681 tccattggtt actgtcagct tttgcgtacc attctcaaaa cgaactgcgc tggaacgccc
     7741 actctgctta tcgatgtcga taactgatcc ggctccttct acgatcaaag aaaggccttt
     7801 tcctacaaaa cgcatcccat tgttcccggg ttgatcgtca tctccagcca ttttgaccga
     7861 gatttgtcca ccacttgata catggatttg actagaatct ccttgcaagc gaaatccgcc
     7921 cgtagttgct gtattgatgg tgattttccc accattcgat acagtgactt tcgatcctga
     7981 acctctcatt tggatgattc cgcgattgtt gtcattggta atattgatct ttgtatttac
     8041 cgaaaattcg cttcccgaac cactgacttc aaacatgaca ttaccaggta gcgtatcgac
     8101 tgcctctcct tcatcgctaa cgccatctgc ccgatcataa tcaagagaga cctttgaacc
     8161 gcttaagata tggattcctg gcgctgtttt tccactacca gtctcatctc ctgaatctag
     8221 aaaaccgtag aatttcccta gatacggtcc ataaagttga ctaccattga tcacattcaa
     8281 ttgatagctt ctacaaaaat aggctttacc cggagcggca ttcagtcctt ccttcactgg
     8341 ttggacatcc aacacaccgg aaacaccatc taatgtgaca gttgcacgag gagcgtaaat
     8401 cagtcctaat ttggacccat catacaaatc aagtgtcccg gtaaatttta tttctcccgt
     8461 aaaatgatat ctgcttcccc aaaaatcggt catataagca atgataatgg catttccatc
     8521 gatatttggt gtcaatccac ctggatgtgc agctgttcct ttaaaattct ggaactcgat
     8581 cgactgaggt atctcattga tataaatatg gtgtgtgccg aaattgtgag cgaatccacc
     8641 aaaatcaata acgatatctc gtttgatctc taattccctg tctaaagtca aaggctgaat
     8701 cagtcggatc ttagttactg attcgtctgc gagaagctgc gccagttctt ctgttgtggc
     8761 agcttcgctg tacccatccg ctgaaacagc tcttgccagt gccgcttcac tgctatcaga
     8821 cgtttgttca gctacttctg aggtctcact tgtcttattt tcttcttgcc ttttattagt
     8881 agaagcctca ctttgagatt ctgtagtttc agaagtaatc gattcttctg aaaaagtagt
     8941 cttgtcaaaa ctttgattct ctgattgcgt tgattcgttt gttttctcta catcggcaaa
     9001 aacggatgtc accatcaaga gagggaagac aaacaaaatc gacgcaatca gcttttttac
     9061 acattgattc attctccacc atcctctctg acgttttgac aattttattt tatcgtttta
     9121 atctatttcc aattgtactt tcatatttaa aatatgtatt tttatggaaa aatttttcta
     9181 ttaggtgtaa atttcttttt tgtacaaaaa aaaggctgtg acaaaagtct tgtcacagtc
     9241 ccttatcccg aacaagcggt gttctcccgc caactcctcg gtcacaacct ccattttaac
     9301 aataaacctg taaatgtgag ggcaatactt tgattgtcac aggtaattta tccccttcat
     9361 ctccatcaac attcgtcgtc aattcttcct catcagctaa tgacacaact aattgctcac
     9421 aagtaaggta ggctagattt ttcgtagatt catctactcc ttttaatagg tctggcaatg
     9481 ttttaagcgt atcgatcaaa gaagaatctt ttaaataaac caaatggagc ttaccatcat
     9541 ccacttttgc atttggaagt aatgtttcaa aaccgccgac tgagttagtt agtcctatca
     9601 gcaaggtact gctctcaatt tcttccttct ttccatcaac ttctacgtga aacgagtagg
     9661 attgtgtgga agctaactgt ttgattcctg acatgaaata agctagtttt ccaaatttcg
     9721 tcttcttctc cgcatctaca tcattgattg attcagggat agagccaatt gcgacgacat
     9781 tcataaaata gtcgtcattt atttttccaa tatccaatgg cttgactgat tcaatcgaaa
     9841 aatgattgat cgcttcctca ggttctagcg gtatccctaa tgctcgagcc aagtcattca
     9901 ccgtacccaa tgggaaaaat ccgaaatttg gccgatgttc ttgttcagcg attccactga
     9961 ttccttcgtt gactgttcca tctccgccca tgacaaacac actatgatac ccttccgtag
    10021 ccgcttcgcg agtaaagttt tttgcatccc ctgctttttt tgtatgcaag acgaccactt
    10081 catcaaacac cgattccaat tttgctatcg ctaattgctc aaattccttt gcttgttcac
    10141 cgcctgaact ggggtttacc actaataaaa cttttttcat cactttcttt cccttctgat
    10201 tgatttcttt aatataacga tagcataaag aaaaaaatta ggctgtgatg aagtactgtt
    10261 catgaaacat tcatttattt tttgacaaga taaaacatcc atactatgaa actgaggaaa
    10321 attaaaacaa tgatatagga aataaaagaa aatgataggc attgtatacc taataaagta
    10381 aaaatcttag gtggcagata aaaacacaaa aatagcaaga gaatgtaata gcgtttcttc
    10441 cactttttgt tatcagacct tatagcgatc agaggaatac caaatgcata actgaaaaac
    10501 tcttgatgta ctgttaatat gtcacggcct tgaataataa atgcgacaat ccccgctata
    10561 attagtgtgc ctaacaccaa aacggaaaat tgatgaatat attttatata caaatgatcc
    10621 attgtacttc ctgccttttt atttgtgaga aaaacaaatc cgtatacgtg aataacttct
    10681 agaaataaaa gaaatttgca aaaccactcc atttatctac ttctacgtgc attttttgat
    10741 attgttttct ttatttaaga ttatacagaa aaatatctag ggtcgctgga ttctaagtaa
    10801 aaaaaatttt taaaataaaa agaactaaat caagtagtaa ttaaactact gttgatttag
    10861 ttctaataaa aagatttaga tgaacagttt tattgattta aatagttcat attttttatt
    10921 tataccgctt atttactact tgctgtacta gaatctgaag tgaatgtact caaaacattt
    10981 tcaaagtctt tgtctttgat ttttacatta gcgtctttca atacttcgcc aattacttta
    11041 ttctggaagg tactgtcact taattttgtg tctgttgcaa tttctttcaa ttcttttttg
    11101 tatttatcca tgtcgttgcc tttgttttgg tttttcacca ttttaaccac ataatattcc
    11161 gtagtataag ttgaagcatt ggtagaagtg atcacatcag aaacttgacc atctttcaac
    11221 ttaaaggctg cttctttgac ttctgccgga accgttgtag tagtggaatc aaatttcact
    11281 tttccgccat cttcttttgt cgtagtatca gttgacttgt ctttggctaa tttagagaaa
    11341 tcgtctccct tatcagcagc tttcttcaca tcttttgcat catcttcact tgctacttgg
    11401 ataatttgag cttccacttc tggatggaaa gattcccaag ccgtttttaa atcttcatcg
    11461 gtcaatttga tatgtttctt tagaccttct tgaaaggcta gactttgttt gatactgtct
    11521 ttgaatgttt gttctgtgta acctgcagat ttcaattgtg aatcaaatga atcacccaat
    11581 tgtttctttg tttgatcata ttgagaatcg atttctttgg ttgatacgtc atctccatat
    11641 ttatcttcaa atacttgata aacgatcaac tgttgcacag cggaatctgc tccttgcatt
    11701 tttttcaagc tatccattac ttcttgttcg ctgattgtac cgcctttata actaacggct
    11761 gttttttcac cggcattcgt acagcctgac aataagccag tgcctagtac gatggcaagt
    11821 gctaaaaatc tttttttcat ttatcaattc tcctactttt cgttttttgc gaattgctaa
    11881 aaacaaaggt gttcaagatg aaaatcatca agaacacctt tactaatctg ctcgatacgt
    11941 cctgaataga tataaggcta cacaatccag aaagttcgct cagcagaata aatgtcaagt
    12001 agcacatcac accttaatat actacttctt ttgttaccgt ttttcaagtc tatcttgcgt
    12061 tccgtattta acgtaactaa ttcttagaac aaatgttatc gattttgttt ttcttcttcc
    12121 gctgctggga tattcaaatc agacactttt atgttcgatt ttccagacat tttttgagag
    12181 tgtaaaatat tttgtgtaaa tagaaaaaag gaagtccctt ctgtagaata gagttaccac
    12241 aacacattca cagaagagag gacttcccta tgaacgattt tactacagaa attctaaaga
    12301 ctctagcgaa caaaggcgat ttgaatgaat tattccgtgt ccatttggag aaagcagtca
    12361 atacgcttct caaaacggag ttaacggctt tcctagatta cgaaaagtat gatcgcattg
    12421 gttttaacac gggtaattct cgtaacggct cctatgaccg tacggtcaag accgagtatg
    12481 gggaacttca tctccagatt ccgcgcgacc gcaatggtga gttcaagcaa cagactgttc
    12541 ctgcttatag acggacgaat gacacgttag aggagaccgt cattcacctc ttccgaaaag
    12601 gtattaccat gtcggaaatc gcagacttga ttgagaaaat gtatgggcat tactacacgc
    12661 cccaaaccat gtccaatata acaaaatcat ttacagaaga ggtaacggcg tttaatgggc
    12721 gggagcttca tgaccgttat gctgctattt atatggacgc aacgtatatt ccgttaaagc
    12781 ggaaaaccgt cgccaaggaa gctattcata tcgcagttgg cattcgcccg gacggatcaa
    12841 aggaagtatt gagctatgcg attgcaccga ctgaatccat cacgatttgg gaggaaattt
    12901 tattggacct tcaagagcgc ggtttgaaaa atgtcctcct gttcatcacg gatggcttaa
    12961 aggggatggt aggagcgatt agtcggttct atcccaaagc tcgttttcaa cattgttgtg
    13021 tacacgtttc ccgtaatatc agtcataaag tgcgtgtcga tgatcgtaag gaagtctgtg
    13081 atgattttaa aatggtgtat caagcctcat ctaaagaggt ggcgttggaa gcacgtggta
    13141 cttttgcgga aaaatggaaa accagctatc caaaagtggt tgaatcgatt ctttcgaacg
    13201 atcacttgct cactttctat gatttcccct tggccatacg caagagtatt tattctacga
    13261 acttgattga atcctttaat aaacaaatca agaaatacag ccaccgcaag gaacagttcc
    13321 aaaatgaaga gtcgatggaa cgtttcttag tctcgtcttt tgatacttac aaccaaaaat
    13381 tcctaggtcg cagtcataaa ggtttccaac aggccgaagg cgagctcgaa caaatgctaa
    13441 gccaactgat tgagaattag aaacaatctg cagtagggaa gaatcattta cacaaaatta
    13501 ttgacgctcc ccattttttt gttgttttta ttatgatata tttttataca actaaaaatg
    13561 aatatattaa aactaccaaa aataactttt aacgctgttt cattatatgt tttttctatt
    13621 ttatataaac ttttggatac ttaataccaa ccggttggat tgtaccaaca tgagaatgtg
    13681 tcagttctat ttcctgtcgc acttgcatag gcccatactc cttttgttgc atttgctgat
    13741 cgaatagagt ttccaacttt cgcttttgca ccgtgacgat tatagacacg actatagtgg
    13801 ttagaatgac ctgtttttct cgtacaagtt gtatcactat aataaaccca tcttccagag
    13861 ctatgattaa ttgctgttct agaaatttcc cggtctactg gtaaatttga cttaataata
    13921 ttacctttag catcaaaatg aatctctcct ggctttaaag atttgtaata gatttcaccg
    13981 tgaactgaat ttgcttccac ttcagatgac atagcgtttc ctccaacaaa acctatccct
    14041 agtgcacaaa taataactgc ttttttcata aaacgacatc ctttcctttt tgatttttaa
    14101 agcatgatct tcttgtttcc tattttggtc agatatggat catgcgtgac tacgataact
    14161 aacgtacctt gggaagcttg ttgcgataag atttcaacta ctttatcctt atttctttta
    14221 tccaatgacc cagtcggttc atcggctaaa atgattttgc tattcttcaa taagactctc
    14281 gcacaggcga ttctttgctt ctcaccacca gataatgtgt agataggcgt acttagcttc
    14341 ttgtttatat ttactttttt caatgcctca tactttgctg ttcgcttatc tgaaacattt
    14401 aaattcgaaa acttcaatcc aatatctaaa ttaaaatcga ttgtttcata ttcaatcaac
    14461 ccaaaatctt gaaaaagaaa agatatggta tttcttttta tcaagcgctt ctctctccca
    14521 gttaattgtt tcgtctcttg atcgaacaaa tagaattgtc ctgaactgaa ctgatcattc
    14581 aatcctaata tattcaataa cgtactttta cctgctccac tttctccaac aacagtcaca
    14641 agatccccag catccgcagt gaaacgatcc aaatgaagaa tcgttttgtt tcgaatgatt
    14701 tttttcgcac cttttatcac tatcaacggc tccaattgtt aacctccatt catcagcaat
    14761 aactgacttt tcttttctgt agacaaaata aaaacaactg aaagaataaa atcaatgaag
    14821 ctaaagaaaa aaatattaaa aagatattta ggctctcttt ctataagaaa aaggaagatt
    14881 acacttgtcg ttccccattt gatgaggttc atcatgaaga aatctttata cgtttcaaag
    14941 agcgaataac cacttgaacg aaagatagcg attttccgac aattgtagac aaaatatatg
    15001 cataacatga catagctcaa caagaagaaa gaaaagaaag tgagtaaaaa tatctgtatc
    15061 aagtaatgaa atgatctgct taacactttg acagctatct cttcagtctg agtatacgga
    15121 aatgataact gcaagttatc acgtaactga ctttcttcaa tcagctcaaa taacctttct
    15181 ttcgacaaag agcctatctt gatctttaac ggtggataga tttctccgct gagaatattt
    15241 ctttccaaat acatactgtt tttcaatgtc agtatttcaa caaccggata atgttcgatc
    15301 caggggatag aaggaataaa tgtataaatg ggttgattgt tttttataaa tagaaatttc
    15361 ggttcttttt cactgatttc ttgataatat tctgttattt gcgccaggtc attttttaaa
    15421 tctgtatgtc tatatcgttc agggatcaat ataacagggt gcgtttcttt ttcggttatt
    15481 tggatcaacc ttccagagac atcaatcaaa ggaaatttct taagataatt cagatttatt
    15541 tttatccctc tgccaaactg ttcattttct tcattcaagt aatctgatac attgactaat
    15601 atacttccgt ttttattcaa agtttcatat aattcctgat ctgctgctga tgcaaaacga
    15661 ttggaataaa taaaatccac gtggtttttc ccgatggtta ctggataaaa tacatgatag
    15721 tcctcaccag cgatttcttt tggtggttca tgggaaacag ctaactgtga agaagaaaaa
    15781 acgatagttg ctaattcgac attggcaacg gtcaaaagaa gagaaaacaa caatccaaat
    15841 gtaaaaagaa tgtattggat atagttgaac gcttctctta acgaagcgat tttttcaact
    15901 aataaaagag aaatcaaaga gataccatat attccgctga acaagaaaac gtacatctgg
    15961 aacaaataaa gaaattgctc cttataatac ataaaaataa aaccaccaat gattcctgca
    16021 attatgaatg taataatgat tttctttttc cccaataaac accacaatac ttgatgtaat
    16081 gaaaagtcat tattcaacaa taaactgatt tctttattca tcaataagaa aaaggatgat
    16141 aacaaaagga ataaaagaat caatccaata gttaaataca ctcgataatt atatacgtct
    16201 gtatttgaaa taaccgaata cgaatagcta ttttcaaagt cttcaaaaat attagtggca
    16261 taatcagtgt gaaatgcttg attgaatcga tcagtgaata gtgaaatgac ccgttcatag
    16321 gcttcttttt ctctcgtcat caagaaaaat tcaccttcaa aatcttttat tttgatcgat
    16381 tcttcaatgg acgttacgcg aaaggaggta ttagaatagg gtaccttctc gtgagaagat
    16441 ttgacatccc cttgataata caatgtcatt tcaagctttg gctgtatctt gaagttccaa
    16501 gatgtatcta cttcattgga ttcatttact gtttttttca tataagaaag ctcagtttct
    16561 gatgagacat tttcaaatac catgattagc tgctcatatt cttcttgccc aataaaatgt
    16621 tgtggcaatc gaatcggagt ataagtatct gtttgatgga aaaattcatt tgtccgatag
    16681 agtacctgtt tatttatact aaccaaggca aaaccactca tgataccggt cactatgatt
    16741 gtgagtaaaa ctgtgaactt ttttttgatt atcaccactc tcccttactt aaatgattct
    16801 ttgataaaat cataccgttt ttcatcaaaa aaacgtatct atcaagtgta taggtcatgt
    16861 agaataaatc tatatactgt agaatcgaaa ggagaagaag atgaacaccg gagaaaaaat
    16921 acgaacttac cgcagaaatt gtaagcttac gcaaaaagaa ttaggcgaaa aaattggtgt
    16981 ttccgataaa acgatttcta gttgggaaaa ttctcgtaca atgccagact ttgaaatgct
    17041 gtcactttta catcatactt taggtctgcc gattgatttt ccttctattg aatcagctac
    17101 cgaatcaata gaaccaacat tgccagatga acctcttgtt aacaagaaat cttattcatc
    17161 aaaacaaccc ttctcactca aaaagacact atctttcttt ctgctcgtct tttgtttggt
    17221 ctatcttttt cttatcaatt atcaatacaa aaatgaaacg atcgatcgat tcaatccttt
    17281 tttgaaacca caaatcggct ataccacact ccctgtagga tacgtggaca attccatcga
    17341 acgttgggta tgtaatgata tctttggcca tggtacatgg ctgtcttttc acgctggaga
    17401 ggtagaaaaa gagaaaaacc atgctgtaat caaacacaaa ggaaaacacg tcatgaacgt
    17461 tcgcctgctc acttgggaag ccattccctc agattaccaa gaaatcctca gccaagacta
    17521 tgacgacgcc ttaaaaagaa gccctcagga gccagatagg cattaagtag gagatgaacg
    17581 tagagataga aaaaatcaat gcacatgatc ttatccaaaa aacaggggta caaattaatt
    17641 gaaaaagcct tgagatgaaa gttcctaaat aaaaaagttc agaatgatag gtatttctgg
    17701 cttatagtat aatatttacg ccacagcctc ccaatgattc tttttggaat cataatcaag
    17761 gatggacatt cacaaaaata ttttgctcat tcgatccgaa tagattaacg accaatctag
    17821 taagtaatat agattggtac gtagtcaagc tgcacataat ttagatcaat tttagttctt
    17881 ttttatattt ctatgatctg ctcatttact tttatatgat ggcttacagt aacataaata
    17941 aatatgataa tcgctggtag tttatccaag agtactcaat aaaatacaaa atggattgtt
    18001 caagcgccta tcataatgat aaaatgaaac aaataaacga tacaaggagg aagagacttt
    18061 gagtaatggc agaacaattt tttcattgta aaaattgttc agttgttaat ctatgggatt
    18121 tcggtagcat gggcattttt ttatagagga atcacaccaa cctggccgtt ttatctcttg
    18181 ttagtgggaa tattagtagg aaattttgcc cctcttcact atcgtacgaa cttttttcca
    18241 actgattctg gcctttttct taaaacaaag acgcgaaaat tagaaaacat tattgaaatg
    18301 acaattacct gtataatcat tttcttagtt atattcctaa acgtcttcta ataagaatct
    18361 cttaaaatta gcttgttaca gttagttacc tagtttatat aacacagtta tcttatcttc
    18421 cttatcaaca tcttttgcca actctttgtc ttaaattcga aacatataag cagagctacg
    18481 aacaccaaga ctgtttcggc tctgtttttt gcatcaaatt cactcttata tagctgataa
    18541 tctcggctct ttaaaagaaa ttattttcgc ttcattttat tataaaaagg ctaggaacct
    18601 tgatgttatc aggttcctag cctttattta agtattaccc cacagaccct tccatataga
    18661 tttttaaatt acacactagt ttaaactaaa acaaacaagg aaaaacttac catttaatca
    18721 aaaaattagc tcatatgaat atattagaat gtaaattaga ctggtcaaaa tctggtcaaa
    18781 aaagaggacg aatacaggta gtcctctttt ttgttatttt gatattctaa atcatttttt
    18841 attggattat tcccttgttc gatatagacc caaattgatc tatcaataat tatcaaactg
    18901 agtcatatag agacaaaaat ttcaaaaatg acctgttaaa tggtcatcga atatcattta
    18961 aaatcaatac gacggagtca gacacggagt caaaacaagc ccgctgagag taaaaaatat
    19021 acagtcccat aggtaaagtg ccacaggact gtatattttt ttatttgaga ttacctgaaa
    19081 tgattccatt taatttttat ctcatttttt cctactagtt agtatcaaat actctcctat
    19141 tttctcaata aactattttg ggttacatgg tatctgaaac agctaaaacc ttgtcattat
    19201 agcaaaaaat cactttatct ccgtttcatt tcagatacat ttcacttaag cggattccaa
    19261 aaaaacaaaa gaaaaaggaa cccctagtta gcctatgctt tttattggtt cctctcaaaa
    19321 cacggtcata taacaacggg gtggggtaaa tgaatgaaaa aggtggagga aacattattc
    19381 aatagctgtc ttaaatactt cgttataaaa tgtgaacaaa taaaagacat ctatccctat
    19441 tttctacact tccacttgac taccatcttt gaatcgtaca attttcttaa ctcttctcgt
    19501 ttacctcaat tcattctcaa gtggaatcaa ctcatcatct aacattttac gccatgctcg
    19561 cttcatttct tgtgggtatt taaatggctt cgcttctgga tgtttggctt tatatccttc
    19621 gtaatcctta ctgttgataa ttgctttcat attaggaatt ggatcttcac ctggtagata
    19681 ttgcagatta gaagcatgta attctttctc agaaacaaac cactcattgg aaaagacatg
    19741 aatcaaatca tcactcgctt gtgtaaaata gcgacgttta atgacaaaga tatcttcatt
    19801 actatctaaa gtccccacat caaatgcatt taaaatttgt cggtaaactt ctttgactcc
    19861 ttctgtttta ttcaatttag ccaaagcttt ttcaatttct tctcgtgcat ctgaactatt
    19921 cgcagcataa gttcctagta attgatcaat ataagttgaa tcaatatcat acaacttagt
    19981 ggtattttca ctataaaatt cgatcgttga aaaatcttct ccttcttttt ctggttcttc
    20041 ctctattaat gaacccttca cggtctcgta cacgccaatt tgttcttcca aaagttgaat
    20101 ttgattgttc aaagttggag atttcaccat gtcatcattg taatcgtcat aagtcactaa
    20161 cgattcataa gcacgattca attcttgaaa tgctttgacg tactcgatac ggatttcaag
    20221 tggcgtatgt tcatcaatgg attccatgga tgaaacctct tttaacttac catatgcttg
    20281 tttgaatctt tttttggatt cctgataact tggataaatc aaacctgatt cttcttttgc
    20341 ttttgaatac agttttgtcg catcctcgac attcttctcc attgtcgctg gcttacgaaa
    20401 cgtcacaatc aatccttttg tcttatcagg atacgtacga ttcgtacgtg aaaaagcttg
    20461 gatcaatcct gcatactcta gattacgatc cacaaatagg gtttgaatcg taggggcgtc
    20521 aaaacctgtt aatagacgat ccactacaat cgctaaatcg atttgtttcc caaattgttt
    20581 gaattccgct ctttttcgtg ccaaacgatt attgatgtct ccgttataac gctcaatgtc
    20641 tgctaatgac caggaagtat cataatactg attatattcc tcaataattt tttgcatttc
    20701 ttcttgttgg gcacttgaat ctcggctatt ttcttctaat gaataggtaa tagcgattcg
    20761 tggaaaatca ggatcctcca tcgttcgtcc ttcacgaatg agctgattgg gaaactcttt
    20821 tgttaaccaa tcaggctctt tcgtcatctc tttgatggcc cgataatact ttttggccat
    20881 gtcaatggaa ctcgtggtta agattgcaga tttcgttggc ctgccatttc gaaaatcaaa
    20941 ttttgtatag gcattatctg gacggaacat tttatgaatg actttttgga tatgttcatc
    21001 tgcttcaaaa acagctgggt ccaaataact ttcttttttg acaggttcca tttgatcaat
    21061 catacgattg atctgttctg atgaatactc tgcatatgtt tctacttctt ttaatttccg
    21121 atagatttta ttctctagtg aagtcggctc cacggtgttt tcatgctcta cttgaaatcc
    21181 taagacagag ccatcttcta gcgcattctt aatggtatag gtatgcaaga cctctccata
    21241 ctgatcatgg gttgtacgtg ccaattgccc tttggcttgt ttccgattct caggaaaaat
    21301 cggtgtacct gtaaagccaa accaagtgga tttcggaaac atttttttga tttcttccat
    21361 attttcagca cttaacgctc gatgacactc atctactaca aacacgatat gttggcccat
    21421 taatttttgg aagcgattgg ttcctttttt ctcttcttgt ttctttgcat atttcaacgc
    21481 cgcatctagt ttttgacggg ttgtaatgat tacaacattt gcattggcat ctgccaacag
    21541 ggtttcactt aattctttgg cacttcccgt tccaacgatc aagctattcg ctttggcgtt
    21601 cccggaagaa atcccagtat taaattccga agcaaatttt gtaaactctg tggtggtttg
    21661 attatctaaa tctttgcggt ccactaacat gatcgttcga tcgatccccg attttcgtgc
    21721 taaaagctta gtagatacaa aactagtcaa cgtctttcct gaaccagtcg catgccacac
    21781 ataccctgat tgatgtttat ttgcggcaat aaacaaggct tgaatcgcat gtacttgata
    21841 aggatgtaac accatgaggg ttttattatc ttgatcttca ctgacgattg tataatccgc
    21901 aatcaaacga tgagcatccg ggatatttaa cacttgttta caaaactcat ataagttttc
    21961 tactttttca ttgtctttcg ttcgccaact aaataaaaac ttagggtgca agtctttcgg
    22021 taaggcattt gcaaaatacc gagtggtttg ttcattggaa accacaaaca actgtaaggt
    22081 cgaaaagata ttgttccgaa acataccttc ttctgcatat tttttgattt ggttaaatgc
    22141 ctgataaaca ccatcttttg cagttacttg tttcagctcg atttggacaa tcggtaatcc
    22201 attgattaat aaggtgacgt caaaacgtcg atcccgtgct tctatgttac ttccacgttt
    22261 tgcaatttga tggaccactt cataagtaga gattcctcca ccaatgtctt gattggaata
    22321 aaggataagt gagacagaac ccaattgcga atcttcccgt tcaatcgtga tccgagccat
    22381 cccattttca ccttttaacc atttcgcagc atcaaatggg gttttcgttc gcagcagtaa
    22441 ttccgttttg atcgtttcaa attctttatc ggtcaaagga aaatcattca gttctgcttg
    22501 attatttgag ataatttttt ggcgtaaatt gacccaaagg tcttcttctg attttagatc
    22561 cggacgatac gtccactgat tatgcccttc tccaagtaca cgaatcaact gttcttcgac
    22621 ttctgcttcc tcacggtgac taattttcat tcttcttccc tcctagacaa acatcttttg
    22681 taagaagctt tttttcgttt cttttagtaa atctaactta cgttgatgaa gagcgatcgt
    22741 atcatctagc tgtttgaaga aagtaccgat tttttgttgt tcatccttta caggaacctt
    22801 aacactgata ttctcaatat cagaactgtt ataagaaggc aatgccccta cttgggagta
    22861 tcttggcaaa tttatagttt caaacaatat tttaccaaaa tagatatccc aagtattatt
    22921 aaataaataa gccatggtat tgttatctat taagaaaggg cttgtgacca aacgttttct
    22981 attaagcatt attgctgcgc caactttagc aaatataatc gctggcacat cttttattgg
    23041 cttccatttt ttacgctcaa tttgctcatt agatacataa tgatttgcat ttctcatttc
    23101 atactcatta ccaatgttat tcatgtcgga aactttaaaa aatggagtac cttcagaacc
    23161 accttgctcg gcatcaggaa atccaattcc cgattgagtt ttacctaatt ctcccaactt
    23221 acgctcttcc caatcttccg taaaaccagg aaaccggatt tctggcactt ttgctccgtt
    23281 ttcagggaac attttttgta agaatccttt tttcgtttcc ttcagcaaat ctaacttacg
    23341 ttgatgaaga gcgatagtgt cgtcgagttt tttgaagaat atgccgattt tttgttgttc
    23401 ttttaaatca ttgggaatat agaatactgt atctgaaact tttttccagt ctgaacgagg
    23461 catttttgtt ccagatgtat catttgcagc cttttgatat ctatcagctt gaataagaga
    23521 ataaataaag tccgagtcgc tcttcttagc tctaaaaacc cagaaatccc caatcgctac
    23581 gcctgtaaaa gttgccttga gccaattttt taaatatgga cgtaactttc catatagaat
    23641 atcatttggt aaaaattcaa tcccttttct attatcaaat ttctggctca catctttatt
    23701 cagtctacct tctcctgcca cgatatcttc aaattcaact tttggaagtc tactggagtt
    23761 tgtactttta ttaagtctaa caactaaatc aattaactta cgctcttccc aatcttccgt
    23821 aaatcctggg aaacggattt ctggtcgtgt atcattgctc atgtttgaac acctccagcg
    23881 caccttgtaa ccacatttcg ttttcaggtg ttgtttgcag ggaagagata gcttctaaaa
    23941 gttctttttc tagcacttgc tgttcttgtc gtatcttctt gatgtcattt ccgacatgaa
    24001 ccatatcaat tggttcttct tcttcgaaag tatctacata gcgtgggata tttaagttat
    24061 agtcattttc tttgatttca tcaaaggttg ccaaatgagc gtatttctcg acatcttttc
    24121 tttcagcgta ggtttcgagg attttttgaa tgttttcttc cgatagttta ttttgatttt
    24181 tccctttaac aaattcacga ctagcatcaa tgaataatac atctcgagtt tgacgattct
    24241 tcttcaacac aatcaccgtc gttggaatcg atgtcccaaa gaacaagttc gcaggcatac
    24301 cgatcacagc ataaatactg ccatcttcta gtaatttttg acggatgacc ccttctgcag
    24361 cgccacggaa taaaactccg tgtggtaaga cgatggccat ggtacctgtt tccttcaaat
    24421 gatagaaccc atgaagaaga aacgcaaaat ctgctttgga ttttggcgct aatttcccat
    24481 aacgattaaa ccgagaatca tctaagaaag tcgtatctgc tgaccaatta gccgaataag
    24541 gtgggttcat tactactgca tcaaatgtgt aaggttcatc tgttggccag tccttattca
    24601 acgtatcccc attgcggaga ttcatttctt cggcgtcgac accatgtaaa atcaagttca
    24661 tttttgctag attgtatgtc gttgtattta gttcttgccc atgatattta acgttatctg
    24721 ggtgtgttaa gtagttacgt acgtttaaca tcaatgaacc tgaacccatc gttggatcaa
    24781 aaacactaaa gaatcgtcgt tccttttgat cgagcgttac gatttgcgcc atcatatcag
    24841 aaaccatatg aggtgtgtaa aattctccgg ctttcttccc tgcttctgaa gcaaattgac
    24901 tgatcagaaa ctcatacgca tccccgatca cgtccccatc gtgttctaaa acatctacat
    24961 cattcaattt cttgatgact tcggtaatcg tcacatttcg ttgttgctca tctgttccta
    25021 gtttttttga ttggagatcc acatcatcaa ataacccatt aaattgattg taggtagatg
    25081 ccaattgcac aaaggctttg tttagatcgt tcaattggaa cgttgcttgt tttgcttgat
    25141 ccgctaagac gttgaaaaga tactctggtg caattgcata tcccaaaata tcaacgattg
    25201 tggcaatcag atcttctttt gattcttcat ctgaaagtaa ttcacgatac aacattgttt
    25261 gtttgcttac tgtgtcatat tcctctaagg attcatctgc taaaagaacc acttgttcca
    25321 acaatcgatc ggataaatat ttatagaaaa tcagtcccaa taaataattt ttatattctg
    25381 acgcatccat tttactacgg agattatctg ccgcactaaa taatttttga tttaattctg
    25441 cacccaaaaa gatacactcc tatctcttat ttaaagttgc tatataccct cttattttag
    25501 cataccccct tttataaaaa gcaaaaaaaa aagaaagaaa cttattcttg ctaataaact
    25561 acgcgaaagg agtctaacgt tgtttgatat aacttagact gatcgttaac ttatttttta
    25621 tatttctaca tagttatctt tagaaccatt tcagatacat atgaaacaat tacatatgat
    25681 tttttcataa aaaaacagta ctgtctagta gtataaaata tacaaatttc ttaaaaattt
    25741 tttcactaaa atcactacct gaaacagtct aaaacactta tatatcaacg tttaatgaaa
    25801 ctatctccgt ttcatttcag atacaaataa atatagtcgg gctccaaatc cgaaaaaaaa
    25861 tacccccagt agcttatcct aagctactgg agatgtataa gtacggtcat ataacaacgg
    25921 ggtagggtaa ataaatgaaa aagatggaag aatcattatt caataacttt tttaaatact
    25981 tcgttgtaat cagttacctg tccgttctta aataccacag aataatcttt ttacccattt
    26041 tcaagtaacc gaagtggaat gtctttacca acttgctctg tcactgcatt actgatacct
    26101 gttagattag ctaataaatc tgataaagat tgagcatctg tagaaatctc tgtatcagta
    26161 tttatcgtga ttcctttatc ttcatttaca agcttccaag taggtggtat agctgtgtat
    26221 gtttcgatga atgaatcaat tctttcatct atcgtcttcc cagtatctac ttctgtctca
    26281 ttactcttag atgaatcgat tctttcatcc atcgtctgct ccgtaactgc ttctgtctca
    26341 ttattctcac cggaaactct cttcacaccc aaacttatat attgtccatc tttagtgtgc
    26401 atagttaaaa ttcccacgct tttactaata aattcactat aaccagttct ttcaatagag
    26461 acttcaatgt atttcacact atcatactca aaagaaaaat agacatgtaa aagcattttg
    26521 ttgtttacat ctacataata atcctttgac tctacttgag accttcctat acggaataca
    26581 gctttactgt ctgatatctc tataccagca tccttattat taatctgcca atctccaagt
    26641 agctcttctg gcaagctagt agtttcttgt gaacacttta ccacttcact ggtagaggtc
    26701 gaaacattca cctccgcact gttagaagaa ttttccaagg aatcaggagc cttagcacaa
    26761 ccaacaagtc tttatatatt gtgcagccga tattttcttc attcagtaca cccttgaaat
    26821 atgcagtgtt agataaagca gttcgtcctg atttgctgag gcacccatct cttcttgaac
    26881 gtactccttg attttcaagg ccgcccgata ctctttcgga aaacgctgct tcactgaagc
    26941 cagcaaaaaa tcatccttct ccggcaactc ttccttctta atcattcggg caataaaaaa
    27001 ctgtaaatgc gtcacaaatc ttaagtaaag catcgaatct ttatcaacct ttttttcggg
    27061 aaacagatat cctagcatag ccaagatatc ctttagtgct cttaccgaga gcatggtttc
    27121 ttccatattt gcttgttcac tttgggcatt tacaatgtga aacgcaatgt ttccagcctc
    27181 ctcttctggc aactgaacat tcattttttt gttgatcaat tggatagcat agcagccgat
    27241 ctcaaattct tttgggtaat acttttgaat ttcaatcagc aggcgatttt gaaaaacaat
    27301 gcctttttga ctgcgttcaa tggcatgctt caagtgatca accaaggtaa aaaatagact
    27361 tttattcagc ttaaagtcaa acttggcgtt ggcataatcg ataattcgct tgacgcaaat
    27421 aattagatca tccggagcag cctcaaacag tttaatgtaa tcctttttta gagcatcaga
    27481 aagtgaatct aatttaaata ctttttggat tttgtcagcc ggaaccaaat cgcctttctt
    27541 tagttgaaat ccaatccctt tccccatcaa aattacttct ttgccttgct tatctaagca
    27601 aagaattaaa ctgttgttta acgttttaat tactctcatt agattcaccg ttctctctaa
    27661 gctaagccat ttcttgcgat tactttttga taccaaaaga agctttcttt ttttattcgt
    27721 tttaattctt taagattctc atcggttcgg tcaacatata taaaaccata acgcttattg
    27781 aagccttgat gggtactaac aacatcaatc gctgaccaag gtaaataacc aaaaacatct
    27841 acaccatctg ttaaagctgc ttgaatagcc agaatatgtt tggctagata atcaattcga
    27901 tcctggtcat taattcgtct atcttcctca actatgtcgg gtgccccata gccattttca
    27961 gtaattaaaa taggtaaatc atagcgatcg tacaatttcc tcaatgttaa tcgtagtcca
    28021 gtagggtcaa ctacccagcc atatggcgtt ttatctacat aaggattttc agctgcccgg
    28081 tagacacctt cttctcccaa catgatctgt tgatcccccg ctctagcgga aacatctgat
    28141 ccgtcatttt tgctggcagc gatggtggca gttgaataat aattaatcgc aataaaatcg
    28201 ggtttaccgg aggataaaat tgcctgatcc tctgcctcaa ttttaggata taatccacga
    28261 tcattcaaat agttttcaaa caaataattg tattttcccc gaactgctac atctaagaaa
    28321 gaccagcctc tcaatgtttc ccaattatgg gctgcaattg catcttctgg actggaggta
    28381 gcttgataca tagccgttaa attgattgct ggaccgattt ttcctttaag attcaactga
    28441 tgatacaagt gaattactct agcttgagct aataaaacat ggtgattaat ttgaaagagt
    28501 tctttttttg aaggcagctc tccacttggt ggcaaaccaa tcgctccagg atgaaggatc
    28561 atcgtgtttt gctcattaat tgtcagccaa tagttcacct tgtcaccata ctcagtaagc
    28621 aatacctgta catagtccac aaaataatcg atagttgctc gatttaacca accgccctgc
    28681 tcctctagaa aataaggtaa atcgaaatgg tacatggtga caatcggttc gattccctga
    28741 ttcaggcatt catcaattac ttgatgataa aaagaaattc ccttttgatt ggttttatta
    28801 ttttcaatat ccataattcg cgtccaagcg atggaaaagc gaaaagcttt taagcctaat
    28861 tctttaaaca gctgtatatc ttctttatag tgatggtaaa aatccgaggc aacttcaaaa
    28921 ttagctaccc cttctgtatg ctgatacttg tcaataatag acggttgttt cccatcctcc
    28981 tgataggcac cctctgcctg gtaggcacta atggaggcac cccataaaaa cttctctgga
    29041 aatgctgaaa gctgtttgta ttgcacaaaa atcctctcct taactaattt gcatcaatgg
    29101 ttcgtgttcc tttaagcttc cgcttgcttc actaattcct gcgtaatttt cactgttggt
    29161 cactaccaca atcactgtat catcgtagcc agcgtttcta atggcagcga aatcggcgtt
    29221 gattaataat tgtcctgctg acacctgatc tcctgctttt acaaatactt caaagggttc
    29281 cccttttaaa ttcacggtgt cgatccccac atggatcaat aattcaatac cttctttaga
    29341 acgtagacca atcgcatgtt tagattctaa aatagtaatg acttctccct caacaggtga
    29401 ataaatgctg ttttcttctg gatgaatggc tacgcccttt cccaatagtc ccttagaaaa
    29461 gacagcatcg ttcacttcag aaataggaca cacggttcct tttaccggat tttcaacaat
    29521 gactgcttcg gctattgccg tttgctctga ttcatcagaa gtatcgtcaa atccaattag
    29581 ataagcaatg ataaaggttg caacaaaggc gattacacac gtaataattg cgtgaacaat
    29641 attcattgga ttttctccaa taaaggctgg taaggctgct aagccagggg aaacaaaggc
    29701 atatctgact aaaccgctca agccagcata caaaccagca attgcaccac caatcatgga
    29761 agcaactaat ggttttttca gcttcaaggt tactccatag agtgaaggct cagtaattcc
    29821 cattaaagca gtgatcccag tggacacagc tagctgcttc aactctttgt ttttagtttt
    29881 cgcggctaca gcaaaggtag cacctgattg agcaatatta gaagccagca ttcctggccc
    29941 attgatcatt tcagaacccg tactgctcaa ttgcatagta gcgattggcg tcattgccca
    30001 agctgtaccc gtaaccacca aaaatggctg caaaccaccc atcaaaaatg gaattaacca
    30061 agctgctttg ccgttaatcc attcagcccc cattgctata agatcgttca gcaacgttcc
    30121 aaacggtcca atgacaacca acgctaaagg ccccataatt aaaaatacca gcatcggctt
    30181 agtaaagaat ttaataaacg aaggcgaaat tcgatccgca aagggctcta cataggacat
    30241 gatccaaaca gtaaagataa tcggtaaaac tgatcccgca taatctgcag ctaataccgg
    30301 aattcctaga aaagaaacgg catcgccagt agaaaaaagc tgtgaaatat ttggatgaag
    30361 caaaatgcca gcaacagtaa tcgccaaaat tgggttgaca tcaaacttta tggctgcccc
    30421 ataggctaat agtactggca taaaataaaa ggctgcatca gcaatagtat tcaaaatgat
    30481 gtaagtttga ctactgtctg atagtacacc agtcaaagtc aagacggcca aaatggcttt
    30541 taccatcccg gcacctgtta tagctggaat catcggtgta aaggtggtag aaatgacact
    30601 aatcaatctt gaaataatac tttcttgatt gtctgaagag gggccctttt taattgaagt
    30661 tcccacttcc ttttgaattt ccttgaaggc tgtttggact tcattgccaa tcaccacttg
    30721 gtattgtcca ccgctatcca ccacactaat tacaccaagt gttttttcta aggctttcgt
    30781 atcggctttc cttttatctt tgagttcaaa acgcagtctg gtggcacaat gggttaagct
    30841 gaccacattt tcttttcccc caacctgttg caatacattt cttcctactt ctctgtaatc
    30901 cattattctc tctccttaaa gcagtaaaaa agaccaagtc aaaccacgcc ccaatcaaca
    30961 ttgaagtgtt agtttgtctt ggtcttgcct gcataatcag taacatgcca ataacgttat
    31021 tttgctgtaa tccaataata gcacattatg atagcgcaat caacagggaa tttaattttt
    31081 tatcttttcg tttgaccgta tagggtaata taacagttag atcacatcaa ctagggtata
    31141 tctgcgtata cttactgcta aaataagtat atctaatatt aactatacca gtaaaaccat
    31201 tcctacataa ccagcgggaa attcccaaaa tcgtgaaagc atcaaaaatg catataacgg
    31261 atcacacaac ttcaacaatt ctgctcttgg gaaaaattga tgaatttggt gagtaaagga
    31321 tggaataaac agcattccaa caatataggc aacaatagtg atacttgttt ttttggtaaa
    31381 aatcagtaag caatagccaa tttcaatcat tccgagatat aaaggtatca atagaatgat
    31441 ttgggctagt attcttaaaa attaactagc aaatgtttaa actgatttat ttctaatcta
    31501 catgtagtaa gtatttcaat tccaccaatt atcaataaaa ataccaaaca tatccataat
    31561 aattttttct ttttcatttc ttttatattg ttataacagt gaataacata aaaattcccg
    31621 acaatcattg taatattttt ttgtcacctg tattagtatt taatttatag gaaactataa
    31681 aaactcccta gtgaatttac acctttggga gttcctgttt ggacggtatg cataatacat
    31741 ttaaatcaat ttaacattaa cacctcagca tatggtatct ccacatatat acggtctttc
    31801 tttggatagt actcaatatt tatgttgata gcacttccat tgtctaatat agctttagcc
    31861 cggtatgtgt tatctccatt ttcatgagta atcgtcacat catcacatga tcgatttaag
    31921 ttgtactgtt cttgtaaaat ctgtgttacc aatggttttg atgacttctc taatccagat
    31981 gtacctgtca tttttgaaaa aataatacct ataatagcca agacaacaat acctataatg
    32041 acaaacttct tgttcatatt tactttattc aactttttat ctcctatctc caaaataatt
    32101 tgttcttatc cctcttatca gtacattgac atccctaaaa gtacaccaaa gattgctcca
    32161 gctacccctg ctatcatcaa aatcgtaccc attttctcat ctttttctcg atataagtag
    32221 cccataataa cagcaacagc accaaataca acaggaataa atgctaacga tactaccata
    32281 gaaatccaac caataatcaa atacagcttc cctgtctctt gtccatgtac gatttgttcc
    32341 ttcatttcat taattgtctg ctcagtactt ttctttactg tgtctgttcc ggttgtctca
    32401 tctgtttctt gtagtgctcc acaaaactgg caatacttac tttcatctgt tatttctttg
    32461 ccacattttc tacaatacat gtcttctcct cctaaaatca ttcaattgct tattagttaa
    32521 ctgtttactt tattaaatac atcagaaaac agctcattca ttggtacact taatatctca
    32581 gaaagcatct tcatttgata gggtttagga atcgttctcc ctgtttgcca cgaacttaca
    32641 cattgctgtg taacattcat tttttttgct aactccgttt gagaaagtcc tttttctttt
    32701 ctgtatcggc tgagattatt tacttttttc atgattctcc cctcctgttt tattatatac
    32761 aacaatatga tgtatttcaa ctcataaaat ataaaaaact tcaaatacca ttacaaatac
    32821 aacaacttgt tgtactatac tgagatgggg gtgtattaat gaaacagaaa caatatgcaa
    32881 aggtcattag agaaaagcga aaagctctcg ggtggacaca aaaagaatta gctgaaaaaa
    32941 tcttctctac acaacaggca gtagcaagat gggaaaattc tgtaacagag cctaacctcg
    33001 atagcttaac agctctttct agagccctag gaacccctgt aagccatttt ctagacaatg
    33061 ttgtcgtaga ttatgaggaa gagtttttgg ctctctatcg ttctcttagc actgaagatg
    33121 cagttcgaac aattgactat atgaagctat taaaaaggca agaaaatgaa cgcaatcagc
    33181 tattgaaaga ttgaaaaata aataacccac cgaactttaa cggtgggtgt attgagatga
    33241 aataagatta agagctatgg ataacctgtc tagtttaata caattgggtc ttttctcttc
    33301 cttactctcc gtccatcatc tcttgttaca cttgtaaatt gatacagagg aactatccca
    33361 gctcctgata atatcaattc ttttattgtt tcatctatcc ctgccccttt cgctaaatat
    33421 attaaaaaac tactacttgc tttcacattg tatagtttct taatatcctt agaagctcgt
    33481 tcaagagtga atccttgttt aacaaagtag cctctgtttt ttattaaagc atttttcacg
    33541 ctgttatccc caacattacg taggcgttca caaacagtgt taataagttc tttctctgtc
    33601 attagatatg tcccttgcca cgataagccc tgtaaaactt catgtgcttc atctaaaatc
    33661 aattgttttt ccttgctgag ttctgatcgg gaattttgag gaaatactct tttagcctct
    33721 ttttcgttaa aacatatctt aacccattca taagaaaaat attgatatgt aaaatgaatt
    33781 ttttccagaa gtggaaggat atcttcattc aatttttgaa tcacagaagt tgcattaaaa
    33841 acgtctaatc gatagacatt gcttctctta tgattaaagt ttcctaacgc tttctcttcc
    33901 tttaacctat ttaggtattc gagcatcttc aaaggaacat cttcatcatc taattttttt
    33961 atcaatccta taaaagtcat aagtgatagt aacttattac atttatctat gtcataagtt
    34021 ttaccatagt aagaactcat ttttttagct aattgttccc ccgacaaaat acaagtcaca
    34081 ctttccttac tatcatcaaa aaaattaaca tcagaaagca agacatcata gacacgtcgc
    34141 atttcctcta gataattcct tattgtcctg aaaagtttgt ctttcattga ttcaagagtg
    34201 tcaaaaagta cttttgattc ctctttcaat tttcgaaaat gtgtaggata caagtctagg
    34261 ttctctaata gccaattaaa tgtttctacc cgactgtctt tctttaaaat tagcatcaca
    34321 actccaataa aattttcact gatatcacaa ttactggaat gacaataaaa aatggaatgc
    34381 ccagttttac tggtaaaaat actagcagag ggattatgat cgttgtgaaa cagacatctt
    34441 aagtttttac ttgacttaag taatttattc atatcaatgg ttaacaatct ttcataaatc
    34501 tcattaaaag acatgtcgga ggaatcaaac aggcagtctt taaaccactg tttcatttct
    34561 tctttgtcgt cattttttat caacttaaca aaatcggttt cattgactag ttcatactct
    34621 ctccctgaat cactttgaca atttttagaa catgtcatgg taaaatcaat cggaagctca
    34681 gtaatatcta acatgttgtc ttcattaatt attatcactg catctgactt atatcctcca
    34741 aaaaacattc tattcgaagc atttacattg tcatcacacc cggggatgtt ctttttcaag
    34801 tattcgtaaa cagcaaccat tttaccatag tcagtaattg cttcttttaa tagaaagaca
    34861 atcttgtatt tttctagatt ttcagagtac cgaatacttt tttgtatcgc aaacgcagaa
    34921 ttatgaacta aattgataga taaaaccttt tctatgctta agtatctact atcatcttta
    34981 ctcaaatcta ctaccttccc cttctcatct ttaacagtgt tatcgacatc cagaataatt
    35041 actctggttt ccacaaaact ctccttgtca ttgcctttat tctttgtctt gcttggaata
    35101 aatgattgtg atgaatttag tatctctaca aactcatctc ttgtaacttc tctatagtta
    35161 tctaaacacc tatctcttac atcagttttt tctttttttg taactggctt ctctaataat
    35221 ttttcttgat ctacataaat tggaatatta atcattgatg tttccatttg tgtttcctcc
    35281 ttataaaatt ctgttaaaga ctaaaacata cagataataa aaacaaactc tttattacta
    35341 tatgttttag tcttatttgt ttactcaacc tttataccgt tccatgaaac tatcaatatc
    35401 agatttttta aatctaatga ccgtatcaat ttttataaca ggtaacccgt attgagaaac
    35461 atattttcta atcgttccta ctgatacacc tagatattca cttgcctgct gatagtccat
    35521 atactctaga gtgactccag aaataatgtc ttctagcata ggctttagca ctgtaattat
    35581 ctctttcact tgttgttcaa cttgtataga aacaatgcta tcaatgattt gtagagacat
    35641 atttccccct cctttcgtga ttgcttgaac aaattttcta agaattatct tcgtattttt
    35701 tgctcaaaat aaaagtattt tgttattaac cgattaatca aacaaaaaat gattaccagt
    35761 tcatcgacat acacataata cctcgataaa aaaggggtgt caacagcttt gtaaaattta
    35821 tttacaacac cccttttttg aggtatacta attttaggag gtgtggtcaa tatgataaaa
    35881 cgtccaccaa taaattattt agaacgaaaa aagatactcg gaacaaaaat aaaagctata
    35941 agaaagagta aaaaattaac acaaccagca tttggtctta tgattaacaa cggtcaatta
    36001 attgacaaaa aaactatcta cgaatgggag aagggaacct atttaccaat accagagcga
    36061 ttatcccgaa ttgctgattt gggaaatatg agtatagagg agttagtttg tggcaatgta
    36121 gaagaatata ttttgggaat tattctttat agagatagca ttgtactaga tggtataaca
    36181 tttccagata aaaacctgtt ccagcatctt cgtcaacagt tcccaccagt acactctaat
    36241 ttagatacat ggctggatag atattccaaa ttagaaccag agatgcaaga atttattgct
    36301 aacaagactt gtaataaagt taaaaatgaa aaaattagtc ttttcaatat cttaaaaata
    36361 gaagaattat tcatcaatgc tattgtagaa gaatttgaca ataacattct tttcctcact
    36421 agcagtatag aagaactact agaaagaatg gtagatgaat ggctacctat tcaattgaaa
    36481 gacatgtctt atcccgaaga agctgtgaga gaaattactg ataatataaa caagctagaa
    36541 caaacgattt catcaattgg gaaaaaatac actaagaaaa aaatgaaggg aggtgatacc
    36601 atctagtggt aagtactcct tgtaaactaa gaggagatga tttaatggca aaaatatcaa
    36661 agtacaaaaa gaaaaacggt caagtggcgt ggatgtataa aggacacatt gccaccgatc
    36721 cacgaactgg tgaaaaaata aatactacga gaagaggttt tagtagcaaa caagaagctc
    36781 aaaaagatta tgacgagtac agacatcaaa tattatacgg tgttaaaaag aaagcacctg
    36841 atatgacatt tgaagacctg tataatgaat ggataactca tcagcgtacc agtgttaaag
    36901 cttcaacaat cgcaatctca gtaagatacg caaataatca aatacttcca gcatttggta
    36961 agcttaaact atcgaatatc tctgtcccat attgtcaaaa agtggtagat gaatggcaca
    37021 gtaaatatga aagttacgat tatatgagaa aacagactgc acaaatttta cgctacgggg
    37081 ttgcaatgca atatattgac aataatccta tggaaaaaac acttcttcca cgaaaaaaag
    37141 agtatgagaa aaatcgtaaa ttttactcaa aagaggagct taataattta ctggatgctt
    37201 ttaaagattt tggtaacatg aaacaatacg ccttcttcag attattggca tacacaggta
    37261 tgagaaagag tgaagtactc gctttacagt ggaaagacat agacacgttt aataaagaac
    37321 tacatgtcaa taaaactctt gctgtagatg aatttggtaa agttataatt caatctccga
    37381 aaacaagagc ttctagacga gttatctctt tagatactga gactctttct atcctcaaca
    37441 actggaaatt acagcaaaaa gaagagtatt taaaacttgg atacaatact agctctaaag
    37501 agcaacatgt ttttacaaca gttaaaaaca ctctatacat tccaaacact gttaatgatt
    37561 ggttaagata tattttgaaa aaatataact tgcctcgtat tacacctcat gggtttagac
    37621 atacacatgc tagtttatta ttagaagcag gagaatctgt caaggtagtt caacaacgat
    37681 tagggcatga aaatagtaaa gtgacattag acatctatgc acatattaca aataatgcac
    37741 ctaagaaaac aggtcaagac tttgctgata tgatggctca tcagtaaaag agctggtcaa
    37801 aatctggtca tttctagtat acaggcaaaa caaaaaagct agaaccctta tatatcaagg
    37861 gttctagctc ctataacagg tttacccaac actgccttcc atctcataac tgatcaatcg
    37921 attgagttcg actgcatatt ccattggaag ttctttggtg aatggttcaa caaagcccat
    37981 gacgatcatt tcagttgctt cagattcggt caggcctcgg ctcatcagat agtagagttg
    38041 ttcttctgag atttttgaga ctttggcttc gtgttctaaa gagacttggc tgttgtggat
    38101 ctcattgaat gggatcgtat cggatttcga ccattcgtcc atgatgatcg tgtcacattc
    38161 gatatgagag atcgaacccg cactgttttt ggcaaaggtt acttgtcctc gatagtttac
    38221 ttctccgccg tcttttgaaa ttgatttaga gacgatagag ctagatgtat ttggtgcatt
    38281 gtggatcatt tttgcacccg tatcttggat ttggtttgct cctgcaaagg cgatggagag
    38341 catggtgcct cgtgcacctt ttccatctaa gtaaacactt gggtatttca tcgttgtttt
    38401 tgcacctagg tttccgtcga tccattcaac ggttgcgcct tcgtatgctt ttgctcgttt
    38461 ggttactaag ttgtacacat tgtctgacca gttttggatc gttgtgtaac ggcaatacgc
    38521 atcttttctt gtaaagattt ctacgatggc ggcatgcagg ctgttacttg aataagtagg
    38581 tgccgtacaa ccttcgacat agtggatgct tgctccttcg tcaacaatga tcagcgtacg
    38641 ttcgaattgc cccgtgtttt ccgcattgat ccggaagtac gtttgtaatg gaacatcgac
    38701 tttgacgcct tttggcacgt aaataaaggt cccacctgac catacggctg aattcagcgc
    38761 tgctaatttg ttgtcggttg gcggtacaag ttttgaaaag tactctttga ataattcagg
    38821 gtattctttc aaagcagagt cagtgtcagt aaagacgatt cctaactttt cgaattcatc
    38881 tttcatatta tggtacacga cttctgattc atattgtgcg gaagcaccgg ctagataagc
    38941 tcgttcagct tctggtatac caatcttttc aaatgtatct ttgatttttt ctggtacgtc
    39001 ttcccagtca cgagcgggtt tgtcacttgg tttttggtaa tatttgatgg catcgaaatc
    39061 gatgtctgaa aggtcggggc cccatgtttg cattggcatt ttgttaaatg tttctagaga
    39121 tttcagacgg aattcaagca tccattctgg ttcgcctttg attcttgaca tctcacggat
    39181 cacttcttct gtcaagcctt ctcctgtact gaaaaccggt tcgacatcat cgtgaaaccc
    39241 gaatttatat tcttctaatt ctggtacgcc catgttctca ctcctcttcc ttgtggtaat
    39301 gatggaaagc tgcggtacca tcgttttcca ttgcttttcc tagtgctttc caggcaagtg
    39361 tcgcacattt gatccgagcg ggaaatttag ctacaccacc aagcatagca gcatcgccta
    39421 attggtcggg atctttgact tcttttcctt ggacaagctg tagaaagtct acagttaaat
    39481 gttctgcttc ttctattgat tttccgatga cggcgtctgt catcatcgag gcacttgccg
    39541 tactgatgga gcagccactg ccgtcaaagg cgatattttt gatgattccg tcttttacat
    39601 cgacttgcaa ttcgatcaca tctccacatg tggggttgtt caattcgatc ttgcttgtcg
    39661 agtcagtcaa gcttccacga tgatgcggat gggaagaatg atccaagatc acttgacggt
    39721 ataaattgtc taatttagat agtcccatgt tggaaaaact cctttgttgc catgattgct
    39781 tcaactaaac ggtcggcgtc ttgcgttgtg ttgtagaaat aaaagctcgc gcgagcagtc
    39841 gctgatactt caaggtactt caatagcggt tgcgcacaat gatgacctgc tctgacggct
    39901 accccttcca tgtctaaagc tgtcgcgaca tcatggggat gtaacccatc caaattgaat
    39961 gcaacgactc ctgtccgaaa attcgggtct tgtggaccat aaacagtcaa tccatcaatt
    40021 gcttgtaatt ttggcaacac ataagcggct agttcttctt catatcgatg gatattgtcc
    40081 ataccgagtt tttgcaaata atcgattgca tggcctaatg cgatagcgcc ggcgatattt
    40141 ggtgtccctg cttcgaattt ccaaggtagt tctttccatg tactgtcgta taattcgaca
    40201 aaatcgatca tttcgccacc aaattcaaca ggttccattt cttctaacca ttttcgttta
    40261 ccatataaaa caccaatacc tgttggtccg cacattttgt gtccgctgaa agcaaagaaa
    40321 tcacagtcta gtgcttggac atcaatggcc atgtgcgggg cagattgagc accatctact
    40381 acgatgaccc cgccgttttc atggacaagt tcagctaatt cttgaacagg attgaagaca
    40441 cctaagacat tggatacatg agcaatggag acgattttgg ttttgtccgt aatctttttc
    40501 gctgcatcct ccatatccag gaagccttct tctgttagtt cgatatattt caagacagca
    40561 cctgttcgtt ttgccaactg ctgccacggg ataacattag aatgatgttc catgtaagag
    40621 agcactatct cgtctcctgc tttgatatag cggtcgccga aggttctggc tacccaattc
    40681 aagcttgtcg tcgtaccacg agtaaacagc acttctgctg tttcttttgc atggatgaac
    40741 gcccgtactt tttctcttgc ttgttcataa tcatgggtcg cgcgttctgc taacgtatgg
    40801 acaccgcgat gaacattggc gttatcgtgt tcgtaatagt acgctaatgc ctggagtact
    40861 tgttttggct tttgggtcgt cgctgcatta tccagataaa ctaaaggttc atcattgacg
    40921 tcttgaaata agataggaaa atcattgcgt agctttttag catcgatcat ccattcaact
    40981 tcccttcgat tacttcgact aattctttcc gaacttgttc tacagggatt gctgttaaga
    41041 cagaaccgag gaatcctcga atcactaagc gctctgcttc ttctttatgc aatcctctac
    41101 tcatcagata gtacatttct tctggatcaa ctcgtcctac actggcagca tgtccagcag
    41161 ttacttcgtt ttcatcgatc aaaagaatag gattcgcatc accacgtgct ttatcagaaa
    41221 gcatcaagac acgactttct tgttgggcat ctgcaccttt tgcccctttc aagatgtggc
    41281 cgataccatt aaaggtcaat gttccttttt ctcggatcac tccgtgttgc aaaatatgcc
    41341 cgatcgtatg aggggcttta ttggttactc gtgtatcgat tccttggatt tgtctgcccg
    41401 agctgattgc cacgattttg acttctgcgt gtgcaccttc tcctgctaaa tctgagtcaa
    41461 agtcagcgat cacatgaccg tcattcatga caccaattgc ccagtcgaca gaagcatctc
    41521 gtaaaatgtg cccacggcga ttcatataag aagtaatatt ttctcctaat tgatctacgg
    41581 ctgaatattt gatttttgaa ccagctttag cgatgacttc cacgatgata ttacctgatg
    41641 attttgctac ttgttctttg gttgtctgga aacgttccag ataagaaaat tcactatgtt
    41701 catctgccac gatcaacaca tgcttgaaga agtgctcatc ggatgcgcca tcttgaatga
    41761 ataaggactc gagtggttca tcgatcacga catttttagg cacatacagg aaaataccgc
    41821 tgttcataaa agctacatgg agtgcagtca attggtcttc gttcatgttt actgcttttt
    41881 tcatataata ttcttggact agttctggat attcgatcat tgcagtgaac aagtcggtaa
    41941 aaatgactcc tttttctgct aattccacag gcagttgttc aaaaatcgtc catgaacctt
    42001 gttggacaag cacaggattg tctttcattg catcaaatgc cggaatcgtg cctgtttcac
    42061 tgatggtttg tgtttctttc acatcaaata acgaccagcg atggaatttc acccgatcga
    42121 tatgcggcaa tggcagttca tctgctttcg ctagtgcgtc ttgacggaag gttctcatcc
    42181 aatcaggttc gcctttactt aaagaaaaat catttaccgc gtcaagataa tcgagccagt
    42241 ttttttcttt cattcttgct cccccttacg cttcttcttc tttatagtcg attcctaatt
    42301 ctttactgat accagcgtag ccttctgctt ccagacgttt agctaggtca gcattccctg
    42361 tcatgactac tcggccttcc atcatgatgt gaacgacatc aggtgtgata tagttcaata
    42421 atcgttgata gtgggtgatg atcaatgcac cgaaattgtc tccgcgcatt tcatttactc
    42481 ctttagcaac gactttcaaa gcatcgatat ccagacctga atcgatttcg tctaaaattg
    42541 caaatgtagg ttctaacatc aacaattgca ggatctcatt ccgtttcttt tctccgccag
    42601 aaaaaccttc gttcagataa cgttcagcca tttcttctgg catatttagt aaagccattt
    42661 tttcatctaa ctttttcaaa aatgccataa cagaaatttt gtcgttttca tcacgtttcg
    42721 cattgatcgc tgcacgcatg aattccgcat tcgtgatacc tggtatctca cttggatatt
    42781 gcatagctaa aaataagcca agtcgcgcac gttcatctac ttccaagtct aaaacatttt
    42841 tcccgtcaaa caaaatttcg ccttctgtta cttcatagtt tggattgccc atgatagcgg
    42901 cagataaggt agattttcct gtgccgttag gtcccatgat ggcatggatt tctccggttt
    42961 tcattgttag gtttacgcct ttaaggattt ctttttcctc gatcgacaca tgtagatttt
    43021 ttatttctaa gacagacatg cattataccc ccaaattctc tttcgttctc actcatttta
    43081 gactaattga gaatgaagcg caatgacctt ccttactaaa atcaaatatc ttttatttat
    43141 tattaaacaa ttttgatttc taaattagaa taggtataaa aaagatgaga aataagccat
    43201 tacagactta tttctcatct tttttgttcc ataccttatt ttgcatcagc ctttactggt
    43261 ttaacagagc cgccccattt gtctgtgatc cattcttgga cttctttgcg gtgaagcact
    43321 tcaatcagtt ttttgatttt gtcactgtct tcatcacctt tacgagtcgc aataatattc
    43381 acgtaaggag agttgtcctt ttcaagcaat acggcatctt ctaatggatt caaccctgcg
    43441 ccgtaagcga agtttgcgtt gattgcaact aggtcacctt catcattttg gtacgtagaa
    43501 gctaaaagtg atgggtcgat cgtatggttg aacttcaagt tcttagggtt ttcttcaata
    43561 tcatcgaatg ttgctgtttc cagatctact ccatctttga ctttgactaa gccagcatct
    43621 tgtagaattg tgatgatgcg tccccagtct gattcagaat ttgaagtaat gattgttgca
    43681 ccatctttta attcggaaac atctttgact tttttagagt acaagcccat tggttcgatg
    43741 tggacagcac cggcattgac aaagtcatat cctttttctt tgatttcttt gttcaaataa
    43801 ggaatatgct ggaagtaatt ggcatcaatg tcgccttctt ccaaagattg gttcggtaat
    43861 acatagtcat cgaatttgac gatttcaaga tcgattcctt catctttcaa taatggtttc
    43921 acatgttcca aaatctctgc gtgcggtgtc ggtgaagcgc cgactttcaa tgtcgtgtcg
    43981 tctgctttat ttgctgaatc tttgctgtcg ctgttgttac cgcatgctgc taatcctaca
    44041 gtcagcaaaa gcgctgctgc aaatccaaaa attttctttt tcatatccta catcccccta
    44101 tatcctatta tcttttgtct gttttcttag ttaagaaatc accgattcct tgaacgataa
    44161 atacgattag taaaataatt aaagtcgcaa cgaatgtcac tgttagatta ccacgctggt
    44221 agccttcctg ccaagcaaga cctccaagtc cacctgcgcc gatcgcgccg gccatcgcag
    44281 taaatccgat catcgagatc gttgtaactg ttaatccaga aatcaatgcg ggaatacttt
    44341 caggtatcaa gaccttccag atgatttccc agtaacttgc tcccattgca tctgctgctt
    44401 ccagtacacc agggctgact tcacgaaaag caatctctac tagacgggca taaaatggtg
    44461 ctgcggataa aacaagagct ggaatggctg cttgcgcacc tagcatagtg cctacgattg
    44521 ctttagtaaa tggaatgatc aaaaccatca ggatcataaa tggcgttgac cggaaaatat
    44581 tactgatgat cgatacgatt ccataaatga ttcgagcata cggtttgttt ttgcgtccaa
    44641 tagagtaaag gatcaatccc aaaactaatc cgatgatcgt cactaagacc attgagatta
    44701 atgtcatata tagtgtatca aggatagcct gattcatcgt ttccgggttg atttgtttaa
    44761 aatctagata tgtttctgaa aagcttttat ccacgatgga tcacctcaat tcctactcct
    44821 tgttctttga tgaacacttc tgcttgatct aattctttgg tttcaccagt catcaagaca
    44881 gtcaatgaac caaaggaacc ttctttcact tgtttgatct ttccatacac aacgttgaca
    44941 tcgatgttat agcgtcgaat cgcttgggaa atgactggct cgttggcatt tctttcgcta
    45001 aaggtcaaag ttgccaaacg gccgcttgga ttttctttga tgagttcagc taatagttct
    45061 gtcgaatcag cttctggttc gatgtcttgt tgtacaaagc gtttggttac ttcttttttc
    45121 ggttgtctga agacagtcaa tacgtctcct tcttctacga ctttccccaa ctccatcaca
    45181 gcgactttgt tgcagatttt gcggatcaca ttcatttcat gtgtgatcaa gacgatggtc
    45241 aaattcaatt tcttattgat cgctaacaat aagtctaata cttcttctgt tgtttgcggg
    45301 tccaatgcgc tagtagcctc atcacaaagc aggatctcag gatcatttgc caatgctcgt
    45361 gcgataccga cgcgttgttt ctgtccgccg gaaagttgtg aaggatatgc ttgtccgcgt
    45421 ccttctaacc cgactaaacg caacagttct tctgcccgtt ctttacgttt gctctttggt
    45481 acgccagcta attccagcgg cagctggata ttttcaagta cagttcttga ccataacagg
    45541 ttgaaatgct ggaagatcat accgattttc tttcggaatg tacgtaattc tttgtttttt
    45601 aattttgtaa tatcttgatc gtttacgatc acttcgccgc tcgtgggaag ctcaagtcca
    45661 ttcaggagac ggactaatgt actttttcct gcgccagaat agccgacgat tccataaata
    45721 tccccttttt caacgtttag tgaaacgtca ttgacggctt ggatgtttcc gtttttccca
    45781 ttgaatgtct ttttgacatt cgttagttgg attaatgaca tgccattcac atctttctct
    45841 tatgattttt caaacaaaaa agccttcgcc ctcttgcctt aagcaaaagg acgaaagctt
    45901 gaacttccgt gttaccacct tgatttaccg ttacttcgca ataacagcct cttccagtac
    45961 ttggacttag cttctttccg tatactgtcg cgctgtaacg ggcactcccg tagtaagttt
    46021 gcttacgctc gcttactctg ctctaagacc atcttccatc tcattttcgt tacccatttt
    46081 cagcaaacag ggctctcttc aaacgtccgt gacatgtact cttcttttca aagctttctc
    46141 gtttcatata ggattgctta tgattatagc aactggaaga atcagtgtca acttttttca
    46201 agtgattttc tgattttata attcgtcaaa ttcttttgga tcaacatctt tgaaacttaa
    46261 gatccacgca ttcatttcat ctgaatcatt caagatattg atatcttcac cgattttatc
    46321 attaacagaa gaaactgtac ccgttagcgg gctggcaaat tcattgaccg ctttttccgc
    46381 ttcaacttcg atcagaggtt catctttttt tagcgattgt cctgcttttg gcagattggc
    46441 aaacgtgatg tctccaagtt cttcttgcgc ttcatttgtc aaaccaacac agtattcttt
    46501 tccattaaat aagatccaaa ggttgtcctt tttcttcaaa tgcttgcctg ccattttata
    46561 tcagttctcc ttcataaatt ttttcattga atccaattgc aacaagctgg tcctctcgta
    46621 agatcagtgg tcgcttgatc agcataccat ctgtcgataa gacttggctc gcttttgaag
    46681 ccgtaaactc gcctacttga tctttcagtc caagttcccg atatttcatc ccgcttgtgt
    46741 tgaacaggcg acggattggt acttgactcg tttctagcca agtctcaatc gttgaggcct
    46801 tcggtacttc tttgatcata tcaacggttt gataactaac gccatgctgg tctaaccacg
    46861 ctttggcttt tttgcatgtt gaacatttcg ggtaccagta aaaggtgtac aattttctta
    46921 cctccttgaa gatcttgcag gtgcttcatt ttggtatctc atcgataata tcaacccgat
    46981 accgatcatg ttccctaaaa tcgaggatcc tccctgacta atgaaaggca atgggatccc
    47041 tgttaaagga agtaatccga tatttgctcc aatattctca aagacatgga acagcagcat
    47101 catgatcaaa ccgcttgcga tataggcgta aaattcatta ttcgtatcga aacacacccg
    47161 aatcatacga tagattaaaa taaaatacaa gaaaatgacg aatgcaccac cgataaagcc
    47221 gaagttttca ccgataacgg taaaaatcat atcggactca cgaacaggca catagatatt
    47281 gcttacgttg aatcctttac caaacaagcc accggtacca attgccaaaa gtccttgggc
    47341 cggctgataa ctcattcctg acgtatcatg gaaagggtcc agccatgaat caatacgtgc
    47401 aaactgataa gatttgaatc caactttata aagcaagtct cttccgcctt cagtcgtaac
    47461 caagaagatc gttcctgctc caacgactac tgccaagatt actacaggtg cgatgatgcg
    47521 ccaagagatt ccagacatca ggaaaatccc gccaaagatc gcaagaaaga caagcatcgt
    47581 tccaaagtca tcttgggcca tgatcaatgc taaaaccggt attgtcacga tcaagagctt
    47641 cccaatcagc caaaagtccg atttcatcgt ccgttctttt acttgcgtat tatgtttagt
    47701 tacgatcagt gccatcatca agatgtacgc aatcttcatc aactctgctg gctggaaagt
    47761 aaacgcaccg aatcgaaacc agtttttaga ccctgttgaa gcagctaaat tctgatcata
    47821 gaatttgagc aagagtccca ttaccactaa ccccaaacca taaaggtaag gagtcagttt
    47881 ccagatccat ttggaattca aatgcatgat gacaacgatt gctgccgctc caactaaata
    47941 ccagacaact tgcatcccta cgcctttcaa gactgctgga ttgttcggat catggctaag
    48001 tgctacatat aatgagagca gaccaatcaa acataacaga aagactggta aaatcacgcc
    48061 ataatcaata cgattatcgt tcgaagcttt taattttttt tccatgtttc atcccttttc
    48121 ttaaaaacca tactgctgat tattatagcg gtccagttag ataatggaaa gtctttccat
    48181 agacatttgg gaaaaattaa ctttccgttt gtgcagttgc ttgagcaaat gcagtacaaa
    48241 agcctactgc ttatctttag tgtatcatag aacttttttt gatacatgca attatctacc
    48301 agctgagatt ctcctcgctg tgagaagttt ctgtgctttc attgtatcga aggatctctt
    48361 cggcaatctc tagcagctga tcagaaacca gattggttcg ttggatgaaa taaaaatatc
    48421 gatggaaatt caacggagtc attggcacat tttctggaac agagcgctta gacaaaatcg
    48481 agcgaccgat tccttccttc agcaacgcaa caattacatc gttacttttc acgatcatct
    48541 tcttatcttg gatattcatc tcttcaaaat aacgctgcgt gtaatgaaaa acaccagagt
    48601 ttgcttctct tacaagccac aatttgcttt ttgggtcccc agcgatgact agctggtctt
    48661 ccatcaaaga gtatctttgt acgccggaag tctctaatgg cttttcgata aaaccaaaat
    48721 cggattcatg tttcgctact tgttctaatg attcatgaga attcgtgatt tcgatctcaa
    48781 aatgcacttt aggaaacctt ttgatcagat gtttcatcaa ctgaggcaaa agatagacag
    48841 caaaggtgtg ggaagcaacg atccgacata cttcttcttc attttgttca ttgagcaatt
    48901 tttgctgagt ttcttcccat tcttctaaca aaaccagtgt ccgttcatat aataagtcgg
    48961 cttgcttagt tggaagcatc tcttttcttc cgttacgttg aaaaagatcg atattcaatt
    49021 cccgctccaa ctgtttgatt tgattcgaga ctgctggttg agataaaaac agtacttctg
    49081 ctgctctgga aaaatttctt gtttcatata ctgctcgaaa agtcgttaat aacttaaaca
    49141 tggcaccact tccataactt tttatgataa ttactataaa aactatttga tttaaatata
    49201 caaaaaacca tctataatag aaatgtacaa caaaacagtt gctgatccaa ctagtaactt
    49261 agatgaagac aatcagctgt ctgcttgctc gtaatcatac attcctattc attttacaat
    49321 gtattcgttc atcttggtac taaaatctat taaataaaaa ggagaatatt atatatgaag
    49381 aactttttga aaattattcc gatcccgatc tgcgggttga tccttgggtt agcttccctt
    49441 ggaaatctgt ttaaagacta tcaccatgtt gctttaggca atctaactgg cggtatctct
    49501 atgattctta tgatattcat ttttgttaaa ttgtttatct tattcgaaca cacgaaacaa
    49561 acgttgcatg atccaatcat cgcctccgtt tcaccaactt ttacgatgtc actcatggtt
    49621 atttgtactt attttgtttc atatgaaagt attgcaccaa ttgtcaaatt catttggctg
    49681 tgcgctgtta tcttccaagt gatccttgtc ttttatttca attatcacca tgttatcaaa
    49741 gctgatcttt caattgaagc aatttatcca agctggttta tcatttacgt tggatttggg
    49801 gtcatcactg taactgccgg gaacttcttc ccacttattg gaaaaatttt cttttggatt
    49861 tcattgatct gctatgcgtt cttattgccg atcatcatca aacgtgtctt tttcgtaaga
    49921 aatatggctc atccgacacg tccgctgatt acgatcgttg cagcacctgg ttctctttgc
    49981 ctaacaggtt atcttaaaaa ctttgctgaa cctaattttt atcttgtcgt agcattgttc
    50041 gctttatctc aagtattgta tttcgttgtg ctgtctatgc ttccacgttt gttcaagctt
    50101 cgtttttatc caagctatgc agcctttaca ttcccgcttg ttatctctgc aacagctata
    50161 tttactactg ttcattactt caacagttta ggtatctata gtagcgtatt ggatttctta
    50221 aaagttgcgg aatgtctgtt tgcaagtgta gttgtcttct acgttcttgc tcattacgta
    50281 cgtttcttaa ttaaagaaca tagcaaacat ggaaaatttg tttctaaaaa aatcgcttaa
    50341 taatcgattc aataggttgt gaaaaagctg ttctgcatca agtaataaga acggccaaac
    50401 aaaaacagct gttcatgttt ttgtactggt tgggcttatt accgcagatg caagcttttg
    50461 aacaccgttt accaagaggt cgtaa
//
LOCUS       DACGLW010000009        49640 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.009, whole genome shotgun sequence.
ACCESSION   DACGLW010000009 DACGLW010000000
VERSION     DACGLW010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49640
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.009"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            <1..198
                     /locus_tag="IXT53_000607"
     CDS             <1..198
                     /locus_tag="IXT53_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4904460.1"
                     /translation="NWFLSNAQSLVLMAIVVIGIYLGFKREFSKLIGFLVIALIAVGL
                     VFNAGGVKDVLLELFNRIIGA"
     gene            281..523
                     /locus_tag="IXT53_000608"
     CDS             281..523
                     /locus_tag="IXT53_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009896970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4904461.1"
                     /translation="MVQKDIFTDMQAKIGCPYLSDLPYYKRTVWFEMKRLCLSDYPKK
                     QLEDFSRYVFGVPYAVIQEALTREDVMKHGRNACAD"
     gene            498..995
                     /locus_tag="IXT53_000609"
     CDS             498..995
                     /locus_tag="IXT53_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAQ4904462.1"
                     /translation="MEEMRVQIEAREPTDGEIRAFWFTLPIDTEQVEELLGIDVDTDY
                     YYITGKELPFADEVQEDTTVEKLNDLYSTYENLPSDLKEDYAELMCYYSNLDELHRYR
                     NDIIHYSWCKNMTDVARHILNNDPAFTSLSESVIRYFDFEAYGQYLDDNGRFVETDHG
                     IYELP"
     gene            1013..1516
                     /locus_tag="IXT53_000610"
     CDS             1013..1516
                     /locus_tag="IXT53_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAQ4904463.1"
                     /translation="MVDDMRVYIANLGKYNEGELVGDWFSFPIDEEDVAERIGLNSYY
                     EEYAVHDTDNFPIEIGEYISIQELNEMYEMICELPDYITDALDEFVSHYGSLEKVYEH
                     KDDIYFYPDCEDMTDIAYYFIDELQVLGEIPLPLQNYIDYEAYGRDLSIEGTFIETSR
                     GICEIPY"
     gene            1680..2069
                     /locus_tag="IXT53_000611"
     CDS             1680..2069
                     /locus_tag="IXT53_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009274602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ4904464.1"
                     /translation="MKKIKSYTGIWNVEKVLYAINDFTLPFPVTFTQITWFVITEFAV
                     ILLGDLPPLSLIEGAFLKYFGIPVALTWFMSQKTFDGKKPYSFLKSQITFALRPKVTY
                     AGKAVKLEKEVFSEPVTAVRSVMYVPD"
     gene            2056..4503
                     /locus_tag="IXT53_000612"
     CDS             2056..4503
                     /locus_tag="IXT53_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ4904465.1"
                     /translation="MFPIKYIDNNLVWNKDNEVFAYYELIPYNYSFLSAEQKFIVHDS
                     FRQLIAQSREGKIHALQIATESSVRSIQEQSKKLVTGRLRDVAIQKIDEQTEALVSMI
                     GDNQVDYRFFIGFKLIVTEEKVSLDSIKKSAFLTFKEFLNEVNHTLMNDFISMPDDEI
                     NRYMKMEKLLENKISRRFKFRRLDKNDFGYLIEHIYGRDGVAYEDYEYSLPKKKLKKA
                     TLIKQYDLIRPTRCLIEESQRYIRLEHEDSESFVSYFTVNAIVGELDFPSSEIFYFQQ
                     QQFTFPVDTSMNVEIVGNRKALSTVRNKKKELKDLDNHAYQSGSETSSNVVDALDSVD
                     ELETDLDQSKESMYKLSYVIRVSAPDLDELKRRCDEVKDFYDDLNVKLVRPAGDMLGL
                     HSEFLPASKRYINDYVQYVKSDFLAGLGFGATQQLGENTGIYIGYSVDTGRNVYLQPS
                     LASQGIKGTVTNALASAFVGSLGGGKSFCNNLIVYYSVLFGGQAVILDPKSERGNWKE
                     TLPEIAHEINIVNLTSDKENAGLLDPFVIMKNVKDAESLAIDILTFLTGISSRDGEKF
                     PVLRKAVRAVTQSDQRGLLHVIDELRREDTAIARNIADHIDSFTDYDFAHLLFSDGSV
                     SNAISLDNQLNIIQVADLVLPDKDTTFEEYTTIELLSVAMLIVISTFALDFIHSDRSI
                     FKIVDLDEAWAFLNVAQGETLSNKLVRAGRAMQAGVYFVTQSSGDVSKESLKNNIGLK
                     FAFRSTDLGEVKQTLEFFGIDKNDENNQKRLRDLENGQCLLQDLYGRVGVVQIHPVFE
                     ELLHAFDTRPPVKSEVE"
     gene            4503..6686
                     /locus_tag="IXT53_000613"
     CDS             4503..6686
                     /locus_tag="IXT53_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ4904466.1"
                     /translation="MIDRIKGLLTRKKLFRFFKIAVIVVLTTIALLSLLGTVAHASGL
                     VDDTVNADNLYSQYPLENYQLDFYVDNSWGWLPWNWLDGIGKSVQYGLYCITNFIWTI
                     SLYLSNATGYVVQQAYKLDFINDMADSIGKSIQTLAGVTENGFGSTGFYVGFLLLIIL
                     IVGIYVAYTGLIKRETSKALHAVINFVVVFIVSASFIAYAPDYIKKINDFSSDISTAS
                     LDLGTKIMLPDSDSQGKDSVDLIRDSLFSIQVQQPWLLLQFGNSDIEEIGADRVEALV
                     SVSPSADDGATREDVVKTEIEDNDNDNLTIPQVINRLGMVFFLLIFNLGITIFVFLLT
                     GMMIFSQILFIIFAMFLPISFLLSMIPSYENMAKQAIVRVFNTIMTRAGITLIVTVAF
                     SISSMFYNISTDYPFFMIAFLQIVCFAGIYMKLGDLMSMFSLNAGDSQSMGRRIFRRP
                     YLFMRHRARRMENRLVRAVGKGSMVGAGTGAMAGSVLSSAGSKQSHANTTRKRDNTSF
                     GSRAGSAVGAVLDTKNKVKDKATAVKENIKDMPTQTAYAVHSAKEKAKSSVSDFKRGI
                     VQGQETRQSERADKLQQHRQNIADKRMELQKAQEAKQAGRKTDGSATTGATRPHDRPV
                     TASPASKTDTGKAQEIKRSVTTDKVHTEPTTPQPVKERPLASGTEKKTSVPVSQPQGQ
                     RQTVRRTVEQTSKTERTLSSQQKQTNYQKNQQTVKKTTTKKGRRK"
     gene            6683..7690
                     /locus_tag="IXT53_000614"
     CDS             6683..7690
                     /locus_tag="IXT53_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009243519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAQ4904467.1"
                     /translation="MKLRHLAIIGSLFPFLIAIVLFFGVLISAEDDDGGGGSSSWVTG
                     MNLSAEVLKHQPMVEKYAKEYGISEYVPYLLAIIQVESGGTAEDVMQSSESMGLPPNS
                     LDTESSIKQGCKYFASLLSSAENQGIEDINVVVQSYNYGGGYIDYVAKNGKKHSFTLA
                     ENFARDKSGGKKVTYTNPIAVARNGGWRYGYGNMFYVELVSQYLTVSQVSGELAQKIM
                     NEALKYQGWDYVYGGSNPNTSFDCSGLVQWCYGKAGINLPRTAQAQYDATQHIPLSQA
                     QAGDLVFFHSTYNAGTYVTHVGIYVGNNQMYHAGDPIGYADLTSSYWQQHLIGAGRIK
                     Q"
     gene            7706..8599
                     /locus_tag="IXT53_000615"
     CDS             7706..8599
                     /locus_tag="IXT53_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008393312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ4904468.1"
                     /translation="MFKKKEKPIKEKKVRTMKVGTHKKSVIALWVVLIASVSFGVYKN
                     FTAIDQHTTHEVETIQLRLNDTNGIENFVKNFAKSYYTWSNTKEAIEARAQAISAYLT
                     QELQDLNVDTVRTDIPTSSTVTDVLIWDVEQSGENTFTVAYEVDQQIKEGDQTRNVSE
                     TYTVTVHVDADGDMVIIQNPTLAPAMEKSDYEPKAQEADNSVDAGTINDATAFLETFF
                     KLYPTATDKELAYYVEGNALEPITGDYLYSELVNPVFTADGDNVKVSVAVKFIDNQTK
                     ATQVSQYELTLHKDSNWKIIE"
     gene            8708..9604
                     /locus_tag="IXT53_000616"
     CDS             8708..9604
                     /locus_tag="IXT53_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HAQ4904469.1"
                     /translation="MQESRLFRIIYHLMENGKSTAPELAEKLEVSVRTIYRDIDIISS
                     AGVPIYVTTGRNGGIQIADSFVLDRLLLSDKEKEDIITALKSVSIVDNHNSDTLSKLS
                     AIFNTKSENWLEVDFSRWGNKSQDNAMFQKVKEAIISHKMLCIVYVNTRGEVIERVIC
                     PLKMVYKAKSWYIKAFCMNKSDFRIFKLTRIIQARNMGKIFNPMEFPQEEKKEIKANC
                     ENVILRFPQEMAYRIYDEFEVDEINQDDNGDFIISATMPIDEWLIGYLLSFGSKVCII
                     EPKYLKKIVYNEAKKICEQNKP"
     gene            9669..10418
                     /locus_tag="IXT53_000617"
     CDS             9669..10418
                     /locus_tag="IXT53_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004610017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ4904470.1"
                     /translation="MNETFMNLTLNNLDTEHICCAISDKKHQIGVSVKKQWLKERIEE
                     GHVFRKLDKKGKVFIEYAPIETAWVPVSGENYLYIYCLWVSGSFKGKGYAKSLLEYCI
                     HDAKETGKSGVCVLSSKKKKPFLSDKKFFLKYGFEVADTAGNDYELLSLSFDGNKPCF
                     SENAKKMTIDQKELTIYYGLQCPYIPNCIEQVQEYCNKNNIPLNLIAVDTLEKAKNVP
                     CIFNNWAVFYNGDFVANHLLNETFLKKKFQL"
     gene            10430..11311
                     /locus_tag="IXT53_000618"
     CDS             10430..11311
                     /locus_tag="IXT53_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005339226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAQ4904471.1"
                     /translation="MHLKKVKGILSSSNGMNLYRGCTHGCIYCDSRSKCYHMEHAFED
                     VEVKENAIVLLENALKRKRLKCMLSTGSMTDPYIPLEDEFSSVRKALSLAYKYGFGFT
                     LITKSNRVLKDLDLLKAINDKTKCVVQMTLTTYDEDLCKKIEPNVSTTKERVEALKKL
                     HDAGIPTVVWLCPILPFINDTEENIKGILNYCIEAKVYGVICFGMGLTLREGNREYFY
                     SQLDRLFPYLKDKYIQTYGMQYQINSPNNATLMKLFHQICEDNGIVHDNKIIFEYLSK
                     FEEKSKGIQLKLFDDIL"
     gene            11364..11771
                     /locus_tag="IXT53_000619"
     CDS             11364..11771
                     /locus_tag="IXT53_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008788676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HAQ4904472.1"
                     /translation="MLVAEFTDKEEHLFNQLVEWLSNTLCTAPVSLSKKYNGLDVNYF
                     TRTVTDDKGDKIHLTAKEFDLLYFLSSHKGQVFTKEQIYENVWGYDNVPDASNLSSFI
                     RKLRLKIEPYPDNPRYIITVWGVGYKFSGEEKP"
     gene            complement(11811..12173)
                     /locus_tag="IXT53_000620"
     CDS             complement(11811..12173)
                     /locus_tag="IXT53_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ4904473.1"
                     /translation="MEIDYKAIGQRIKIARIKKGITQETVADLIDITPAHMSNVETGK
                     TKVSLPTLIEIANALSVSVDVLLCDNVLASQNIFKGEAKEIFEDCDEYETRFLVDLLK
                     CAKTAMRRDKNVRNQFNR"
     gene            12324..12548
                     /locus_tag="IXT53_000621"
     CDS             12324..12548
                     /locus_tag="IXT53_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4904474.1"
                     /translation="MMKFSKRDSRKMIKEMARLHGLSVSEVREQIQGKIIDVMNSDDP
                     EQQAEFKRLFGNSTPTPEEFICVTSRQLKF"
     gene            12568..12933
                     /locus_tag="IXT53_000622"
     CDS             12568..12933
                     /locus_tag="IXT53_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015535453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4904475.1"
                     /translation="MGQVEKQKEVFLSVCGQHDYDLLTGKEAMTQTDFERITYITMVL
                     GYTTYTQELISEHLDLACNEAERTDREIDILQDYPAYYDDENVYEQRDKWLADFIGQV
                     PPAKQDDIRQLIKENTEII"
     gene            13374..13802
                     /locus_tag="IXT53_000623"
     CDS             13374..13802
                     /locus_tag="IXT53_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-24"
                     /protein_id="HAQ4904476.1"
                     /translation="MKRPTSPEDVQKVFDCYCKKILKNEAINIQKHYQRMNDLQISFS
                     ELTPEQLAELSTYDDYSTDYDLYKVMGYDVKIKNELLSEALQELSEEERFILLLYGLG
                     FNDGEIADLLKIVRRTVNYKRQRGFEKVRKRMEEKQHEEE"
     gene            13789..14037
                     /locus_tag="IXT53_000624"
     CDS             13789..14037
                     /locus_tag="IXT53_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ4904477.1"
                     /translation="MKKNKAVHKGNGLLPYPVIVSASQGDILAMNVVLCHYEPYIARL
                     SMRTSIDEYGNPHTSVDEDMRSRLEAKLIEQVLNFKVA"
     gene            complement(14123..14347)
                     /locus_tag="IXT53_000625"
     CDS             complement(14123..14347)
                     /locus_tag="IXT53_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009274596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ4904478.1"
                     /translation="MSKTTSGTYHYPVNTGHGGATFPKVALGSLRGSLALFWKGHGVQ
                     SPWLAVSQTYLSALFSCQRPQRLIRLSKPI"
     gene            14437..14643
                     /locus_tag="IXT53_000626"
     CDS             14437..14643
                     /locus_tag="IXT53_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HAQ4904479.1"
                     /translation="MTNNTDVPIWEKYTLTIEEASKYFRIGEKKLRKLAEENLDAGWV
                     IVNGNRVQIKRKQFEKIIDTLDEI"
     gene            14723..15919
                     /locus_tag="IXT53_000627"
     CDS             14723..15919
                     /locus_tag="IXT53_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015535449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ4904480.1"
                     /translation="MSNVKRKDNKGRNLRLGESQRKDGRYVYKYTDIYGNPQFKYAWK
                     LVPTDKTPAGKREDLSLREKEKQIKKDLADGINTIGGKMTVCQLYAKKNSQRKNVKRN
                     TEKGRQYLMKILENDPLGARSIDTVKLSDGKEWAMRMYENGFSYKTISNYKRSLKASF
                     YMAIEDDCIRKNPFSFKLSDVLEDNSEPKVILTPEQEEKMLAFMEQDKTYSKYYDEVV
                     ILLETGLRISEFCGLTVHIDMANRIINVDHQLLKDSEIGYYIETPKTKKGERQLPMTE
                     RAYQAFKRVLQNRGKAEPIIIGGYSNFLFLNQKGFPKVAGNYESMLRGLVKKYNKTHK
                     DQLPNITPHSFRHTYCTNMANKGMNPNTLQYIMGHANITMTLGYYAHGTLNSAKAELE
                     RLALAS"
     gene            complement(16320..16754)
                     /locus_tag="IXT53_000628"
     CDS             complement(16320..16754)
                     /locus_tag="IXT53_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4904481.1"
                     /translation="MQTQEAIKTFILKKKSNTKLDIFLFLSEHRFFITTQYLADHFHM
                     SESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESV
                     SYQILTSLFSCQTNSIISFSQQTNYSASYLYTKMKKINAFLA"
     gene            16902..18043
                     /locus_tag="IXT53_000629"
                     /pseudo
     CDS             16902..18043
                     /locus_tag="IXT53_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(18169..19248)
                     /locus_tag="IXT53_000630"
     CDS             complement(18169..19248)
                     /locus_tag="IXT53_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ4904482.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(19351..19791)
                     /locus_tag="IXT53_000631"
     CDS             complement(19351..19791)
                     /locus_tag="IXT53_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ4904483.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(19848..20258)
                     /locus_tag="IXT53_000632"
     CDS             complement(19848..20258)
                     /locus_tag="IXT53_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAQ4904484.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(20438..20872)
                     /locus_tag="IXT53_000633"
     CDS             complement(20438..20872)
                     /locus_tag="IXT53_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ4904485.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(20972..21820)
                     /locus_tag="IXT53_000634"
     CDS             complement(20972..21820)
                     /locus_tag="IXT53_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ4904486.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            22209..23975
                     /locus_tag="IXT53_000635"
     CDS             22209..23975
                     /locus_tag="IXT53_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HAQ4904487.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            24077..25336
                     /locus_tag="IXT53_000636"
     CDS             24077..25336
                     /locus_tag="IXT53_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ4904488.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            25483..26784
                     /locus_tag="IXT53_000637"
     CDS             25483..26784
                     /locus_tag="IXT53_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ4904489.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            26787..27638
                     /locus_tag="IXT53_000638"
     CDS             26787..27638
                     /locus_tag="IXT53_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ4904490.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            27651..28466
                     /locus_tag="IXT53_000639"
     CDS             27651..28466
                     /locus_tag="IXT53_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HAQ4904491.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            28617..29783
                     /locus_tag="IXT53_000640"
     CDS             28617..29783
                     /locus_tag="IXT53_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ4904492.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPTQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            30013..30660
                     /locus_tag="IXT53_000641"
     CDS             30013..30660
                     /locus_tag="IXT53_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HAQ4904493.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            30744..31688
                     /locus_tag="IXT53_000642"
     CDS             30744..31688
                     /locus_tag="IXT53_000642"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HAQ4904494.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            31823..33274
                     /locus_tag="IXT53_000643"
     CDS             31823..33274
                     /locus_tag="IXT53_000643"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HAQ4904495.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            33295..34131
                     /locus_tag="IXT53_000644"
     CDS             33295..34131
                     /locus_tag="IXT53_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAQ4904496.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            34131..34790
                     /locus_tag="IXT53_000645"
     CDS             34131..34790
                     /locus_tag="IXT53_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ4904497.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            34916..35605
                     /locus_tag="IXT53_000646"
     CDS             34916..35605
                     /locus_tag="IXT53_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4904498.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            35611..36567
                     /locus_tag="IXT53_000647"
     CDS             35611..36567
                     /locus_tag="IXT53_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HAQ4904499.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHRIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            36611..37123
                     /locus_tag="IXT53_000648"
     CDS             36611..37123
                     /locus_tag="IXT53_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ4904500.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(37379..37927)
                     /locus_tag="IXT53_000649"
     CDS             complement(37379..37927)
                     /locus_tag="IXT53_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ4904501.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(38016..39197)
                     /locus_tag="IXT53_000650"
     CDS             complement(38016..39197)
                     /locus_tag="IXT53_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HAQ4904502.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(39433..40941)
                     /locus_tag="IXT53_000651"
     CDS             complement(39433..40941)
                     /locus_tag="IXT53_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HAQ4904503.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            41100..41291
                     /locus_tag="IXT53_000652"
     CDS             41100..41291
                     /locus_tag="IXT53_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4904504.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            41494..42411
                     /locus_tag="IXT53_000653"
     CDS             41494..42411
                     /locus_tag="IXT53_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HAQ4904505.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            42404..43477
                     /locus_tag="IXT53_000654"
     CDS             42404..43477
                     /locus_tag="IXT53_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4904506.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            43625..44143
                     /locus_tag="IXT53_000655"
     CDS             43625..44143
                     /locus_tag="IXT53_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4904507.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            44124..44516
                     /locus_tag="IXT53_000656"
     CDS             44124..44516
                     /locus_tag="IXT53_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HAQ4904508.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            44592..45527
                     /locus_tag="IXT53_000657"
     CDS             44592..45527
                     /locus_tag="IXT53_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HAQ4904509.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            45660..46439
                     /locus_tag="IXT53_000658"
     CDS             45660..46439
                     /locus_tag="IXT53_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HAQ4904510.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            46432..46749
                     /locus_tag="IXT53_000659"
     CDS             46432..46749
                     /locus_tag="IXT53_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAQ4904511.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            46993..48603
                     /locus_tag="IXT53_000660"
     CDS             46993..48603
                     /locus_tag="IXT53_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ4904512.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            48760..49608
                     /locus_tag="IXT53_000661"
     CDS             48760..49608
                     /locus_tag="IXT53_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ4904513.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT        16052 a         9173 c        10274 g        14141 t
ORIGIN      
        1 aactggtttt taagcaacgc ccagagctta gtgcttatgg caatcgtggt aatcggtatc
       61 tacttaggat ttaagagaga gttttctaag ttaatcggct tccttgtgat tgctttaatc
      121 gctgtcgggc tggtattcaa tgccggcggt gtcaaggacg tgctgttaga gctgttcaac
      181 cgtatcattg gggcgtaaca atcagcagtt tgcggtcacg caaacttacc tgtcccccta
      241 tgggagttcg ggcatagggg gaatgaaagg gggtgacgga atggtacaaa aagacatttt
      301 tacagatatg caggcaaaaa tcgggtgtcc gtatctctct gacctgccct attacaagcg
      361 tacagtctgg tttgagatga aacgcctttg cctgtctgac taccctaaga aacagttaga
      421 agatttttca cgctatgtat tcggtgtgcc ttatgccgtc atacaggaag cgttgacaag
      481 agaggacgtg atgaaacatg gaagaaatgc gtgtgcagat tgaagccaga gaaccgacag
      541 acggagaaat ccgagccttt tggttcaccc tgcctatcga cacagaacaa gtggaagaac
      601 tgctgggaat tgatgtagac acagactatt actatatcac aggcaaggag cttccctttg
      661 ctgatgaagt gcaggaagat accaccgtgg aaaagctcaa tgacctgtat tctacttatg
      721 aaaatttacc ctctgacttg aaagaggatt atgcggagct tatgtgctat tactctaatc
      781 ttgatgaatt gcacagatac agaaatgaca ttattcatta ttcgtggtgt aagaacatga
      841 cggacgttgc ccgccacata ctgaacaacg acccagcgtt cacttccctt agtgagagcg
      901 ttatccgcta tttcgatttt gaagcctacg ggcagtacct tgacgacaac ggacgctttg
      961 tggaaaccga ccacggtatc tatgagcttc cgtagaaaag aggtgtgtgc ctgtggttga
     1021 cgacatgagg gtgtatatcg ctaatcttgg caagtacaac gagggcgaac ttgtagggga
     1081 ttggttctct ttccctatcg acgaggaaga tgttgcggag cgtatcgggc tgaacagcta
     1141 ctatgaggaa tatgctgtcc atgataccga caactttcct attgaaatcg gggagtatat
     1201 ctccattcaa gaactcaatg agatgtacga gatgatttgt gagcttcccg actatatcac
     1261 cgacgctctg gacgagtttg tttcccatta tggaagtctg gaaaaagtat acgaacacaa
     1321 ggacgacatt tatttctatc cagactgtga ggacatgacc gatattgcct attacttcat
     1381 tgacgagtta caggtattgg gtgaaattcc gctaccgttg cagaactata tcgactatga
     1441 agcatacggc agggacttat ccatagaggg gacattcatt gaaacaagcc gagggatatg
     1501 cgagatacca tattagagct ggcagatcag cgacagtaaa ggtcgctact gacagctttt
     1561 tgtattttac ggggacggcc agaagcgggc tgtcccttaa tcattgaaag aaatcccaca
     1621 aggggatacc tctatgccgt gaggaatacg gcagggataa tgaaaggagc tgaaacgaat
     1681 tgaagaagat taagagctac acgggtatct ggaacgtgga gaaagtctta tacgcaatca
     1741 atgactttac tctcccgttt cccgttacct ttacccagat aacgtggttt gtcatcacgg
     1801 agtttgccgt cattctctta ggggacttac cgccgttatc gcttatcgag ggggcgtttc
     1861 tgaaatactt cggtattccc gtcgccctca catggttcat gagccaaaag acctttgacg
     1921 gcaagaagcc ttacagcttc ttgaaatcgc agattacatt tgccctgcga ccaaaagtga
     1981 cctatgcagg aaaagccgtg aagctggaaa aggaagtttt cagtgaacct gtcacggcgg
     2041 ttaggagtgt gatgtatgtt cccgattaaa tatatcgaca acaaccttgt ctggaacaag
     2101 gacaatgagg tattcgccta ctatgagctg ataccgtata actattcttt tctgtcagcg
     2161 gaacagaaat ttatcgtcca tgacagcttc cgtcagctta tcgcacagtc ccgtgaggga
     2221 aagatacacg ccttgcagat agcgacggaa agctccgtaa gaagcataca ggaacagtca
     2281 aaaaagctgg tgacggggcg acttcgtgat gtggcgatac agaagataga tgaacagaca
     2341 gaagcgttag taagcatgat tggggacaat caagtggact accgcttttt tatcggcttc
     2401 aagctcattg tgacagagga aaaagtgagc cttgacagca ttaagaaatc ggcgtttctg
     2461 acgttcaaag agttcttgaa tgaggtcaac catacgctga tgaacgactt tatctccatg
     2521 ccagatgatg aaatcaaccg ctacatgaaa atggaaaaac tgctggaaaa taaaatctcc
     2581 cgccgtttca agttccgacg cttggataag aacgacttcg ggtatcttat cgagcatatc
     2641 tacggcaggg acggcgtggc gtatgaggac tacgagtatt cgcttccaaa gaagaagctg
     2701 aaaaaggcaa cgctcatcaa gcagtacgac cttatccgtc ctacccgctg tctgatagag
     2761 gaaagccagc gatacataag gctggaacat gaggacagcg aaagctttgt gtcctacttt
     2821 accgtcaatg ccattgtggg cgagctggat tttccgtcca gtgagatatt ttacttccag
     2881 caacagcagt tcacctttcc cgtggacact tctatgaatg tagaaatcgt cggcaaccgc
     2941 aaggcacttt ccacggtgag aaataaaaag aaagagctga aagacttgga caatcacgcc
     3001 tatcagtctg gaagcgaaac aagctcaaac gtggtggacg cattagacag cgtggacgag
     3061 ctggaaaccg acctcgacca gagcaaggaa agtatgtata aactttctta cgttatccgt
     3121 gtgtctgctc ccgacctcga cgagctgaaa cgacgctgtg atgaggtcaa ggacttctac
     3181 gacgacctca atgtaaagtt agtccgaccc gctggggata tgctggggct tcactctgaa
     3241 tttctccctg ccagcaagcg ttacatcaat gactatgtac agtatgtgaa atcggacttt
     3301 ctggcggggc ttggctttgg ggcgacacaa cagcttggcg agaatacagg tatctatatc
     3361 ggctattccg tggacacggg aagaaacgtc tatttacagc cctctcttgc ttcacagggt
     3421 atcaagggga cagtcaccaa tgctttagct tctgcctttg tcggttcgct gggcggtgga
     3481 aagtcattct gcaacaatct gattgtctat tattccgttc tctttggggg acaggctgtc
     3541 attctcgacc ctaaaagcga gcgtggcaac tggaaagaaa cgctcccaga gatagcccat
     3601 gaaatcaata tcgtcaatct taccagcgat aaggaaaatg ctgggcttct tgacccgttc
     3661 gtcattatga agaatgtaaa agacgctgaa agtctggcga tagacattct cacattcctt
     3721 acggggattt cctctaggga cggcgagaag ttccctgtcc ttagaaaagc ggtgcgtgcc
     3781 gtgacccaga gcgatcagcg aggacttctc catgtgatag acgagctacg cagagaggac
     3841 acggcgattg cccgcaatat tgccgaccat atcgacagct ttacggacta tgactttgca
     3901 catctgctgt tttccgacgg ttcagtgtct aacgctatca gcttggacaa ccagctcaat
     3961 atcatacagg ttgccgacct tgttctgcca gataaagaca cgacctttga ggaatacacg
     4021 accatagaac tgctgtccgt ggctatgctg attgtcattt ccacctttgc ccttgacttt
     4081 atccacagcg acagaagcat ttttaaaatc gtggatttag atgaagcgtg ggcgttcctc
     4141 aatgttgcac agggagaaac cctctcgaac aagctggtac gagctggacg tgctatgcag
     4201 gcaggcgtgt acttcgtcac gcaatcctct ggggacgtgt ccaaagaaag cctaaagaac
     4261 aatatcggct tgaagtttgc ctttagaagt accgacttag gcgaggtaaa gcagaccctt
     4321 gaatttttcg gtatcgacaa gaacgacgag aacaaccaga aaaggcttcg tgacttggag
     4381 aacggacaat gcctgcttca agacttgtac gggcgtgtgg gcgtggtgca gatacaccct
     4441 gtctttgaag aattactgca cgcctttgat accagaccgc ccgtgaaaag cgaggtggag
     4501 tgatgataga taggataaag gggcttctga caagaaagaa gctctttcgt ttttttaaga
     4561 tagctgtgat tgtggtactg acgaccattg ccctgctctc ccttttaggg acggtggcac
     4621 acgcttctgg gctggtagat gatacggtca atgcggacaa cctgtattca cagtaccctt
     4681 tggagaatta ccagcttgac ttctatgtgg acaatagctg gggctggctt ccgtggaact
     4741 ggctggacgg tatcgggaaa tccgtacaat acgggcttta ctgtatcacc aactttatct
     4801 ggactatcag cctgtatcta agcaacgcca cggggtacgt cgtccagcag gcgtataagc
     4861 tggacttcat caacgacatg gcagacagta tcggaaagag catacagaca ttagcgggcg
     4921 taacggaaaa tggctttgga agtacgggct tctatgtggg cttcctgctt ctcattatcc
     4981 ttatcgtggg tatctatgtt gcctacacgg gacttatcaa gcgagaaacc agcaaggcac
     5041 tccacgctgt tatcaacttc gttgtggtgt ttatcgtgtc cgcttccttt atcgcctacg
     5101 ctcccgacta tatcaagaag ataaacgatt tttccagtga tataagcacc gcttctctgg
     5161 atttgggaac aaaaatcatg ctccccgaca gcgacagtca aggaaaagac agcgtggact
     5221 tgataaggga cagcctgttc tccatacagg tacaacagcc gtggctactt ctgcaatttg
     5281 gcaacagcga tatagaggaa atcggggctg accgtgtgga agctctggta tcggtcagtc
     5341 cgtcagcaga cgacggggcg acccgtgagg acgtggtgaa aacggaaatc gaggataacg
     5401 acaatgacaa tctgacgata ccgcaggtca tcaaccgtct gggaatggtg ttcttcctgc
     5461 tcatcttcaa tctggggata acgatattcg tattcctgct cacaggcatg atgatattct
     5521 cccagatact atttatcatt tttgctatgt tcctgcctat cagcttcctg ctctccatga
     5581 tacccagcta tgagaatatg gcgaaacagg caatcgtgcg agtgttcaat acgattatga
     5641 caagggcggg gataacgctc attgtcacgg tggctttcag tatttccagt atgttctaca
     5701 acatatctac cgattacccg ttttttatga ttgcgttctt acagatagtc tgctttgcgg
     5761 gtatctacat gaagctgggc gaccttatga gtatgttctc tcttaacgct ggggacagcc
     5821 agagcatggg acgcaggata ttccgtcgcc cgtatctgtt catgcgacac agggctaggc
     5881 gtatggaaaa cagattggta agagctgtcg gaaaaggaag tatggtaggt gctggaacgg
     5941 gagcaatggc tggttctgtc ttgtccagtg caggcagtaa acagtcccat gccaatacga
     6001 cccgtaagcg tgacaatacg tcctttggaa gccgagctgg ttcagcggta ggtgcggtgc
     6061 ttgatacgaa aaacaaggtc aaagacaagg caacggctgt caaggagaat atcaaggata
     6121 tgccgaccca gactgcctat gctgtacact ccgcaaagga aaaggcaaaa tccagtgttt
     6181 ccgacttcaa gcgtggcatt gtgcaggggc aggaaaccag acaaagcgag cgtgctgaca
     6241 agctccaaca gcatagacag aatatcgctg ataagcgtat ggaacttcaa aaggcacagg
     6301 aagcaaagca ggctggaaga aaaacggacg gatcagcgac aacaggagct acccgtcccc
     6361 atgatagacc tgtcaccgct tctcctgctt caaagacaga cacaggaaaa gcacaggaaa
     6421 tcaagcgttc tgtcacgacc gacaaggtac ataccgagcc gaccacgcca cagcctgtca
     6481 aggagcgtcc gcttgcttct ggtacagaaa agaaaacttc tgtccctgta tcacagccac
     6541 agggacagcg acagaccgta agacgtaccg tggagcagac aagcaagaca gagcgtactc
     6601 tttccagcca gcagaaacag acaaattacc agaaaaatca acagaccgtg aagaaaacta
     6661 ccacaaagaa aggacgtagg aaatgaaact aagacatctc gccattatcg gcagtctgtt
     6721 tccattcctc attgcgatag tgcttttctt tggcgtgctt attagtgccg aggacgacga
     6781 cgggggcggt ggttcttcct catgggtaac gggcatgaac ctgtccgcag aggtcttgaa
     6841 acatcagcct atggtggaga aatacgcaaa ggaatacggc atatccgagt acgtccccta
     6901 tctgctggca atcatacagg tggaaagtgg cgggacagcc gaggatgtta tgcagagttc
     6961 cgagagcatg ggcttaccgc ctaactccct tgatacggaa agctcaatca agcaggggtg
     7021 caagtatttt gcgtccctgc tttcttcagc agaaaatcaa ggcatagagg atataaacgt
     7081 cgtggtgcag tcctataact atggcggtgg ctatatcgac tatgtggcta agaacggaaa
     7141 aaagcacagc ttcacgctgg cagagaattt cgcccgtgat aaatctggcg ggaagaaagt
     7201 cacctacaca aaccctatcg ctgtcgcacg caatggtggc tggcgttacg ggtacgggaa
     7261 tatgttctat gtggagcttg tcagccagta tcttactgtc agtcaagtat cgggcgagct
     7321 ggcacagaag ataatgaatg aagctctgaa atatcaaggc tgggactatg tgtacggcgg
     7381 gagcaatcct aacacttctt ttgactgttc ggggctggtt caatggtgct atggcaaggc
     7441 gggtatcaat ctgccgagaa cggcacaggc acagtatgac gctacccagc atatcccgct
     7501 ttcacaggca caggctgggg atttggtgtt cttccacagt acctacaatg cgggaacgta
     7561 tgtaacccac gtcggtatct atgtgggaaa taaccagatg taccacgctg gcgacccgat
     7621 aggctacgct gaccttacaa gctcctactg gcaacagcac cttatcggag ctggcagaat
     7681 taagcaatag aaaggaatgg aaatcatgtt caagaagaaa gaaaaaccga taaaggaaaa
     7741 gaaagtccgt actatgaaag tggggacgca taagaagtcc gtcattgccc tctgggtggt
     7801 gcttatcgca agcgtgagct ttggtgtgta taagaatttt acggctatcg accagcacac
     7861 cacccatgaa gtagaaacca tacagctccg cttgaatgac accaacggca tagaaaactt
     7921 tgtcaagaac tttgcgaagt cctattacac atggagcaac accaaagaag caatcgaagc
     7981 aagggcacag gctatcagtg cttatctgac acaagaattg caggatttga atgttgatac
     8041 tgtgagaaca gatataccga ccagctccac ggtcacggac gtacttatct gggacgtgga
     8101 acagtctggg gagaacactt ttactgttgc ctacgaagta gatcagcaga taaaagaggg
     8161 cgaccagaca aggaatgtat cagaaaccta taccgtgacc gttcatgtag acgctgacgg
     8221 ggatatggtg attatccaga acccgaccct cgctcctgct atggaaaaat ccgattatga
     8281 accaaaggca caggaagccg acaacagtgt ggacgctggc acgataaatg acgctacggc
     8341 atttctggaa acattcttca agctgtaccc taccgcaacg gacaaggagc ttgcctacta
     8401 tgtagaggga aatgcccttg aaccgataac cggggactac ctctattccg agcttgtcaa
     8461 tcctgtattc acggcagacg gcgacaatgt gaaagtgagc gttgcggtga aattcattga
     8521 caatcagaca aaagcgacac aggtatcaca gtatgagctg acgcttcata aggatagcaa
     8581 ctggaagatt atagagtgac gataagtaaa gactcgctaa tttcatatct tttggcgagt
     8641 ctttacctga taaacttgac atatatatgt caggcttata ttatgaaaaa gaaatggagt
     8701 gatttaaatg caagagagta gattatttag aattatttat catttaatgg aaaacggaaa
     8761 atctaccgct ccagagttgg cagaaaaatt agaagtatca gtaagaacaa tttataggga
     8821 tattgatatt attagtagtg cgggtgtgcc tatatatgtg acaacgggaa gaaacggtgg
     8881 catacagata gcagatagtt ttgttttaga taggttgctt ttatctgata aagaaaaaga
     8941 agatataata acggcattaa aaagtgtatc tattgtggat aatcataaca gtgatacatt
     9001 gtcaaaacta tcagcgattt ttaatacaaa aagtgaaaac tggcttgagg ttgacttttc
     9061 acgctggggg aataaatcgc aagataatgc tatgtttcaa aaggtaaagg aagcaatcat
     9121 atcacacaaa atgctttgta ttgtttatgt gaatactcgt ggagaagtga ttgaaagagt
     9181 tatctgtcca cttaaaatgg tgtataaagc gaaaagctgg tatataaaag ccttttgtat
     9241 gaataagtcc gatttccgta tctttaaatt gacacgtatt atacaagcaa ggaatatggg
     9301 gaaaattttt aatcctatgg aatttccaca agaagaaaaa aaggaaataa aagcaaattg
     9361 tgaaaatgtt atactgcgtt ttcctcaaga aatggcttat cggatttatg atgaatttga
     9421 agttgatgaa atcaatcaag acgataatgg tgattttata atttctgcta caatgcctat
     9481 agatgaatgg ctgattggtt atctgctttc ttttggttca aaggtttgta tcatagaacc
     9541 aaagtattta aaaaaaattg tctacaatga agcaaaaaaa atatgcgaac aaaataaacc
     9601 atgacatatg ttgtcaaggt tattgtgata taatacagat gaaaaagtat taaaggggtg
     9661 aactatttat gaatgaaaca tttatgaatt taacgctgaa taatctggac acagaacata
     9721 tatgttgtgc aatttcggat aaaaaacatc aaattggagt atcagtaaaa aaacaatggc
     9781 ttaaggaacg aattgaagaa ggtcatgttt tccgtaaact tgataaaaaa ggaaaagtat
     9841 ttattgaata tgctccgata gaaacagcat gggttcctgt ttctggagaa aactatctgt
     9901 atatttactg cctttgggta tctggctctt ttaagggaaa aggatatgca aaatctttac
     9961 ttgaatattg tattcatgac gcaaaggaaa ctgggaaatc tggtgtatgc gttctcagct
    10021 caaaaaagaa aaagcctttt ttatctgata agaagttttt tctgaaatat ggttttgaag
    10081 tggcagatac ggctgggaat gattatgagt tattatcatt gtcttttgat gggaataagc
    10141 cttgtttttc tgaaaacgca aaaaaaatga caatagacca aaaggagctg acaatctatt
    10201 atggattaca gtgtccatat attcctaatt gcattgaaca agtacaggaa tactgtaata
    10261 aaaataatat accattaaat ttaattgctg tagatacttt agaaaaagct aaaaatgttc
    10321 cttgcatttt caataattgg gcggtatttt ataacggtga ttttgtggct aatcatcttt
    10381 tgaacgaaac ttttttgaaa aagaaatttc aattataaag gtatagttta tgcacttaaa
    10441 aaaggtgaaa gggattttat cttcatcaaa tggaatgaac ttatatcgag gttgtactca
    10501 cggctgtata tattgtgact caaggagcaa atgttaccac atggaacatg cttttgaaga
    10561 tgttgaagtc aaagaaaatg ctattgtcct tttagaaaat gctttaaaaa ggaaacgatt
    10621 aaaatgtatg ttgagcactg gttctatgac agatccttat attccgcttg aagatgaatt
    10681 tagtagtgtc agaaaagcat tatcgttagc atataagtac ggttttggct ttactctgat
    10741 aacaaaatct aatcgtgtat taaaggattt agatttatta aaggctatca atgataaaac
    10801 aaaatgtgta gtacaaatga cattgacgac ttatgatgag gatttatgca aaaaaattga
    10861 accaaatgtc agcacaacaa aagaaagagt tgaagctcta aaaaaactac atgatgctgg
    10921 tattcccaca gttgtctggc tttgccctat tcttcctttc atcaatgata cggaagaaaa
    10981 tatcaaagga attttaaatt attgtattga agcaaaagtt tatggtgtta tctgttttgg
    11041 aatgggacta acgcttcgtg agggaaatag agaatatttt tatagccagt tagacagatt
    11101 gtttccgtat ttgaaagata aatatataca aacttatggt atgcagtatc agataaacag
    11161 tccaaataat gcgacattga tgaagctttt tcatcaaata tgtgaagata acgggatagt
    11221 acatgataac aaaattatct ttgaatattt aagcaaattt gaagagaaaa gcaaaggtat
    11281 tcagttaaag ttatttgatg acatattata gaataattgt tcttatgaaa tttatttatg
    11341 aaagttgttg atattatggg aaaatattag ttgctgaatt tacagataaa gaagaacatc
    11401 tttttaatca gttagtagaa tggctgtcta atactttgtg tactgcacct gtttctctaa
    11461 gcaagaaata taacggttta gacgtaaatt attttactcg taccgttact gacgataaag
    11521 gagataaaat acatctgact gccaaagaat ttgacctgct atatttcctt tccagccaca
    11581 aaggacaggt atttacaaaa gaacagattt acgaaaatgt gtggggatat gacaatgtac
    11641 cagacgcaag caacctttcc tcattcatca gaaagttaag actgaaaata gaaccatacc
    11701 cagacaatcc acgatatatc atcactgtct ggggcgtggg atataaattc agcggagaag
    11761 aaaaaccgta gctgtatttc acaattcatt gtgatctgca cgctacggtt ttatctattg
    11821 aactgatttc ttacgttctt atccctacgc atggctgttt tggcacattt cagcaggtca
    11881 actaaaaatc gggtttcgta ttcgtcgcaa tcctcaaaaa tctcttttgc ttctcccttg
    11941 aaaatattct gtgaagcaag tacattatca cataggagaa catcaactga tacggaaagg
    12001 gcattggcaa tttcaatcaa tgtcggcagg ctgactttcg ttttccctgt ctccacgttg
    12061 ctcatgtggg ctggggtaat atctataagg tctgcgacgg tttcctgtgt tatgcctttc
    12121 ttaattcgtg ctatcttgat acgctgtccg attgctttat agtctatttc cacagtttcc
    12181 ctctcctttc ttgcttttca ttattgtaac tattcacaga gtgtaatata atagtctgta
    12241 tagatgaaac atttatgtac tatatttttg gcgaattttt tgaaatatat catcaatttt
    12301 gagaaaagag gtgttctgac cgtatgatga aattcagtaa aagggatagc aggaaaatga
    12361 taaaagaaat ggctcgtctg catggacttt ctgtttcaga agtccgtgaa cagatacagg
    12421 ggaaaatcat agacgtaatg aacagtgacg acccagaaca gcaggcagag tttaaacgac
    12481 tgttcggcaa cagtacgcct acaccagagg aatttatctg tgttaccagc aggcagttaa
    12541 aattttaatg atttaggagt ggctattatg ggacaagtgg agaagcaaaa ggaagtattt
    12601 ctgtctgtat gcggacagca tgactacgac ttactgacgg gcaaggaagc aatgacacag
    12661 acggattttg aacgtatcac ttatattact atggttctgg gctatactac ctatacgcag
    12721 gaattgatta gtgaacattt agacttggcg tgcaatgaag cggaacggac tgacagggaa
    12781 attgatattc ttcaagacta tccagcctat tatgatgatg aaaacgtcta tgaacagaga
    12841 gataaatggc tggcagactt catcggtcaa gtaccgccag caaagcagga cgatattcgc
    12901 cagctcatta aagaaaatac agagattatc tgatacacag ggttatcgca atcacggcat
    12961 ttatgctggg tgtgatagcc ttttttagtt cctgctaaaa aacaagaact tggacggaat
    13021 taggacggat ttatctttta tgcttattct gttgaaattt gccttatttt ggcaaggcgg
    13081 tttcttcacg ggaggattac gaatgacagg agtgcataaa agaaaatcat caaaaaaatt
    13141 ttaatccgtg ttaagggagt ttacagctat ttccgtgtag aatttcctca atatttgtcg
    13201 attatattcg ctaaagctca acgaggttat caaaacttct tgggcttttt tgctttttct
    13261 ggatttcttt ttcaaacttt gccagttcat atcctctcat ttgtattaat gacggaaagg
    13321 gggaaggaac ggttacaatc accgcctttt gacaagcgaa aggaggtgaa aatgtgaaaa
    13381 gacccacttc tccggaagat gttcagaaag tctttgactg ttactgcaag aagattttga
    13441 agaatgaagc aatcaatatt cagaagcact accagcgtat gaacgatttg cagatttcct
    13501 tttcagaact cacgccagaa caacttgcgg aactctcgac ttatgatgat tattccacgg
    13561 actatgacct ttacaaagtc atgggctacg acgtgaaaat caagaatgag ctgttaagtg
    13621 aagcattgca ggaactttct gaggaagaac gctttatctt gttgctctac ggtttaggct
    13681 tcaacgacgg cgagattgcc gacctgctga aaattgtacg ccgtacagtg aactataagc
    13741 gtcaaagagg atttgaaaaa gtcagaaaaa gaatggagga aaaacagcat gaagaagaat
    13801 aaagcggtac ataaaggcaa tggcttattg ccttaccctg ttatcgtttc cgcttcacag
    13861 ggcgatattc tggctatgaa tgttgtgtta tgccattacg agccatacat tgcccgcttg
    13921 tccatgcgaa caagcattga cgaatacggc aatcctcaca ccagcgttga cgaggatatg
    13981 cgtagccgtc tggaagcaaa actgattgag caggtgctta atttcaaagt agcataaacc
    14041 aacgtcctat tacagagtga cgcattatcc cctttcctgc gtcctctctg gggcttgtat
    14101 atctatcaaa gtctatatga cctcatatgg gctttgacag gcgtataagc cgttgcggtc
    14161 tttgacaact gaataaagca gacagatacg tttgtgatac agcgagccac ggggactgta
    14221 cgccatgacc tttccaaaag agagctagcg acccacgcag tgatccgaga gcgacctttg
    14281 gaaaagtcgc cccgccatga cctgtattca caggataatg atacgtccca gaggtggttt
    14341 tgctcatagg aatgagattg gttgagatac cagcggagct tttccagagc cgtctgtctg
    14401 cttccaaaag acacacagta aagggggtgc ttatacatga cgaacaatac tgatgtgcct
    14461 atctgggaaa agtacacgct gacgattgag gaagcgtcaa aatacttccg tatcggggaa
    14521 aagaaattgc gtaagttggc agaggaaaat ctcgacgctg gctgggtgat tgtgaatgga
    14581 aaccgtgtgc agattaagcg aaaacagttt gaaaaaatca ttgatacatt ggacgaaatc
    14641 taatgtcagc gagccgtaga tatgatataa taaagtataa cgtatcaacg gctcattcct
    14701 tgacggaaag gagcttcgca ctatgtctaa tgtaaagaga aaagacaata agggacgcaa
    14761 tttacgactt ggagagagcc agagaaaaga cggaagatac gtttacaaat ataccgatat
    14821 atacgggaac ccacagttta agtatgcttg gaaacttgtt ccgacagaca agacacctgc
    14881 agggaaacgt gaagatttgt cgttgagaga aaaagaaaag cagattaaga aagaccttgc
    14941 cgacggtatc aataccatag gcgggaaaat gacagtctgc cagctctacg caaagaagaa
    15001 cagccagaga aaaaatgtga aacgcaacac agaaaaaggt cgccagtacc ttatgaaaat
    15061 tctggaaaac gaccctctgg gtgcaaggag cattgataca gtgaagctat cagacggtaa
    15121 ggaatgggct atgcgaatgt atgagaatgg cttttcctac aagacgatta gcaattacaa
    15181 gcgttcactg aaagcgtcat tctatatggc gattgaggac gactgtatca gaaaaaatcc
    15241 tttcagcttc aaattgagtg acgttctgga agataattca gagcctaaag tcattctcac
    15301 gccagagcag gaagaaaaaa tgcttgcctt tatggaacag gacaagacgt acagcaagta
    15361 ttatgatgaa gtggttattc ttctggaaac ggggcttcgt atttctgaat tttgcggtct
    15421 gacagtccat attgatatgg cgaaccgtat catcaatgta gaccaccagc tattgaaaga
    15481 cagcgaaatc ggctactaca ttgaaacacc taagaccaaa aaaggagaac gtcagcttcc
    15541 aatgacagaa agagcctatc aagcctttaa gcgagtgtta cagaacagag gaaaggcaga
    15601 gcctatcatc ataggcggtt acagcaattt cctgttccta aaccaaaagg gcttccctaa
    15661 agtggctgga aactatgaga gtatgttgcg tgggcttgtg aagaaataca acaagacaca
    15721 taaagaccag ctacccaaca tcacaccaca ctcattcaga catacctact gtacgaacat
    15781 ggcaaacaaa ggaatgaacc cgaacaccct gcaatatatc atgggacacg caaacatcac
    15841 catgacatta gggtactacg cacacggtac tttgaattca gcaaaagcag aacttgaaag
    15901 gctggctctt gcttcttaaa tcggtgattg tactactcat ttactacttt tggttgcatt
    15961 ttcatgctgg taaatgccag cttatgcaga gtatcttcca aaaggaaaat gccgataaag
    16021 ccctaaaata caggctatac cgacatttac cagcttatga aaagataatc agaaaattag
    16081 tgagttttta atagaaaata taatttatct atcataccgc tatttagtcg gtcaagtact
    16141 tcttttttaa aaaaagtatt catcataaaa gccactactt gcggatcttc atcataaaat
    16201 tctgtagcag tacttgaaaa gatgttccaa taaatatcta atgctgcata ttgaatttgt
    16261 acctcttttc cagaaaaaga ctttctgcca ttattagaaa aggagataga caaaccgtac
    16321 tatgctaaaa atgcattaat ctttttcatt ttcgtataca aataagaggc actatagttt
    16381 gtttgctgac tgaaactgat aatcgaattt gtctgacagc tgaacaaaga agtcaagatc
    16441 tgataactga ctgattcgtt gcagtatctt ccaaatagac gataatagca gtaagccgga
    16501 tcaattcctt caaaattcaa ctttaaaaaa ggtttttgct tatcaatatg cagttctgtt
    16561 agattgagac gctcaaagtc ttgttctaat tctttgatgt atagtaaaaa atttgattca
    16621 gacatatgga aatgatctgc taaatattgt gtagtaataa agaaacgatg ttcagatagg
    16681 aaaagaaaaa tatctaattt tgtattgctt tttttcttca agataaacgt tttaatcgct
    16741 tcttgagttt gcatgaaaga ggctcctttt tacttaaatt gttaaaactt gaaaatatta
    16801 ctgttttttt cataaattta gacaaaaaaa cagtaaacat ataggtagaa tctctactat
    16861 cttatttctt gcatacaaaa aaatcaagga gtgagagagt tatgagatat tcaaccatct
    16921 taatatcaac atttgcagcc ttaagcttat cgcttactat aaatcagaca gtccacacag
    16981 aggagacaga aacagccaaa tggacagaaa ccaagtgggc caacgtcaat cgaattacct
    17041 ttcaggatga attttcatcc ttcaattcag gagtcgcaaa attaagaaaa ggacaaacag
    17101 cgaccatcaa agcaaaaatt gactattcgg gagacttagc acctatacta caatcagctg
    17161 ttacatttga gaatgttgat cccactttgt cgattggcta tgacgagaac actttgacta
    17221 tccaacacaa gcaagcgata tttaccttca ccgttacatt aaatcaggac ttgttaaaac
    17281 cagcgctttt tactgtcaag gtatcagatg gactaccgaa cgatcatcat ttaacgccgt
    17341 atagtcagcg gcagacaatt gagcaagatt cagccatcga ctcaggagga gatcttgttg
    17401 aagaaccaac agataaacca gaaaatgaaa ataagccaga agtaccgcct acagaaaatc
    17461 cagacggaga acagaaaccg gaaatagaac ctggagaaga acccgataca gaaactcaac
    17521 cagagccaga caataaatca aaacctgaaa taacccctgg agaaaaacca gatgtaactc
    17581 cagaaccaga tacggattct ggaaaccaaa cagtaccgga aacaaatcca gacacagata
    17641 atgaaactga aaatccagaa aaaccagagg tagatccaga agaaaaacca gatgtgactc
    17701 cagaaccaga tacggacgca agagaccaag gaataccaga aaaaataaac aaaaaaacaa
    17761 ttcaagagga tggaaaaaaa gagagtaaaa aatcaaatct agcaattcta aaaataaatg
    17821 aagagcagct gaataaaaaa tcacgaatat ttgatagcgc ccaatcagct gaaaccttaa
    17881 aaagtagcaa ggatacaacc tttgctgtcc tgaaacgaaa aataaacagc taccaaaatc
    17941 cggagagtca caaaacaaag ttatactgtg gagcggaatc attctattaa gtatagcaac
    18001 aatgctttct gcaaaaagat ttaaacaaaa cagatctctc taacatttga ataatgtctg
    18061 tgaacgcatt tttcaaataa aaagaaatat ccagaaaatg accccaaaat accagattga
    18121 aatctgatat ttggggtcgt tcatacttta tctttcttca tatttatttt aagggatatc
    18181 tgtttctttt ggaagatggt tcgggatctt tttcactttc aatacatcta aaaagaaggt
    18241 aaagatcgga atgccgacaa tcaagcccca aacgccaaag aaccgttcgc taacaagtaa
    18301 gaccacaaat gtataaaaaa tcggcagttc tgttttgctg gacatcaact tcggattcag
    18361 cacataagac tcaattagat ggacaatcaa aatcgtaatc aggatataaa caacatctcg
    18421 gatgcctcct tgggaataag ctatgaaaga tagaggaata caagaaatga taacacctgc
    18481 tactgggatc agacttaaaa tgaaaatcat gatcgctaaa ctcagcagct gatgaaagcc
    18541 aaatacagcc aaggcgattg tagtgagcaa cgtatttaaa agagcgacaa caaattgagc
    18601 ttctaatacc aaaccaaatg tattgacaaa cttatcggca aaatagtaaa tatcttgaaa
    18661 gaaccaagaa aattcacttt ttaaaaacaa tcttgagaat acaattgttt ttttcttttc
    18721 aatcataaaa aagaaactta agatgaatga catagcaaac gccactgcca tttttcctat
    18781 atcctgtaaa tagccaagcg caatcgaagc gccattttga agctgttcaa aaaaatcgtt
    18841 tcgctcaata taagtatgga tatattgtaa aacaggatct gcatcttttg gctgatgctc
    18901 gtaaaaatta ataacggact ggatcatgtc aaatgtttga tgaataagca tcgggatata
    18961 ctttgtgaat gccagataga taaggtaaac gatcagtgta taaatcatga tagctaaaac
    19021 acctgtaggg attcggacaa atcgctgaat gatttgaaca agatgaatca tcaaataagt
    19081 aaagataaat gtgagcaaaa tggttgtaat catacttcta actaagaaaa gaacaaaaac
    19141 gacaaataat aaaacagtga gacggcgtat tttttcattc gccaaaaaac gctgataaac
    19201 ggtcaaatcg acaacctcca aataacaaaa aataaacacg ttattcatta tatgatggaa
    19261 tatgaaaata agaaagagca aaaagaaaaa ccgctaaatg acgttatctt atgagttggt
    19321 tttcctcata aaatcgttca tctagcagtt tcagcccttt ttctggtctt gatgaaaatt
    19381 atctagcatt tttcgggtca gatgttgcag tgtatccaat tcttcctgtt caagtggaag
    19441 ataagacttg atcttttgtg gaataccttc tgcttgtttt tgcaacgcag caccttgagc
    19501 agtcagcgta acatttacga ttctttcatc acccgtttca cgctgacgat taatataatt
    19561 tttttctgct aactttttta gtaagggagt cagcgtacca gaatccagac acaaatcatt
    19621 tcctagctct ttcactgaag tctttttctt ttcccataaa actaacatca caagatactg
    19681 ggtataggta agattcagcg gcttgaggag gggagtgtag tgtctaatga tttctttaga
    19741 ggcggcatat aaaggaaaac atacttgatt aactaaatat agatcttcca tttttccacc
    19801 tccgctgttt tactatagtg tatcatagcg gatttacaca aaaaagtcta ttcactaact
    19861 gctttcagag taacttctat atttccagct gtagcttttg aatacggaca cactttatgc
    19921 gctgcctcta ataattcttg cgtttcttcc agagaaagac cttcgataga tccttcaatc
    19981 tctacaccta agacaaagct cgtttcactt tctgaataaa gggaaacttg tgcactgaca
    20041 gtagaagccc cagagatatt tttttgctgt ttgatcaatt ctaatgcact gttaaaacat
    20101 gcactgaaac cagctgcaaa caattgttca gggttggttg cattttcgac gcgtttacca
    20161 ggagctgcaa tattcaattc gaatgtacga tcgggattat gcacttctcc agagcgtccg
    20221 cctgtgttga tcattttcgt agaatacatt tttttcataa aattaccacc tttaattata
    20281 ttttatacaa ttgaatttta cacaatataa ttgtggaaag caaagaaatg gattggcata
    20341 agataaataa gagaaagcac atgaaacaga cgcgctttct cgaatagcaa acatatacaa
    20401 aaaatatcag agacaaaaaa taatctcatc ataaacatta catatatttc tcaaaaagtt
    20461 gttttctttc ttcaaaaatt acttcacgat ggacctgatg aagcatccat aaataaccaa
    20521 aaggatctct gaacattgag ttagtcacac caaagtccat catttctgtc atttcttgaa
    20581 tctctacaca gccatttcgt aatgctttat tatgtgtgtc ctcgatatca gagacgatga
    20641 tattgaacca gatcgtattt ggatgttcag cagtaggagc agtcaaacca agagacgtgt
    20701 tttcatccag catatggaat ctcgtaccat acaaggtaaa aatgacttca ttattgcctc
    20761 tagggtaatc tgttgcctca atcagttgta tttcatcaaa gattgtttga tacagttcta
    20821 gtgctttcaa actatcattt aatacaaaat cgatttccgt tcctttatgc ataaagatct
    20881 cctctccttt tcagtcgaac agttactaga ttcttttatt ataccagcta aagaaaacgc
    20941 attcaaaaaa atagcggatt tacttaccaa ctcattcgat tggatgataa gtaaatccgc
    21001 gtcccattac ttctgctacg ctgctgacaa tgacaaaggc acttggatcg atttcttcta
    21061 tcaatggttt cattcgggaa aattcatgat cagtcacaac gatcaataaa acctcttttg
    21121 actttcgatt atacccgcct tttgcctcta gcagggtaag gccacgtcca atctcagcac
    21181 ttaagcgaag gcggatgtct tccaaataat gttcactcat gaccataaca gatttctttc
    21241 gattgatacc tgtttcgata taagtcatga caattgaagt gattgcaata gataaaatag
    21301 cataaagaaa ctcttctatt ccaaaaataa acagattaag agaaacaacg aatgcgtctg
    21361 taaaaaataa gccaatcgaa gtattcaagt ggaaatattt tttaaaaatg aggggaggga
    21421 tcgtcgtacc gccgctagaa gagttgtttt tatataaaat cgcaactcct aaagcaaaaa
    21481 tcgcacttcc aaaaatgaca gacaacaacc gatctgaagt aatcattgtt tctggaacta
    21541 ttgcaatgat taaaggaaaa accaagcttc cgaaaagtac tttaaaaaag ggttttttac
    21601 ctaaaaatag aagagctagt accaacataa cgatattcaa cactaaaata actatagctc
    21661 ggtcaaagcc taaagcagct tccagtaata ttcctagacc gctgacacct ccagcagcaa
    21721 tatgatgagg ccctaaaaac atattgatag cgactcctaa tatgaagagg gcaaaaataa
    21781 tcttaatgta tgtccatata aattgtctcg tttctttcat ttattcactt ccttaattca
    21841 ttaataatac tcttatctta ttataactta cttttcacct taagttaact ttaattaata
    21901 aaaaaattag gctagtctaa attattatgt ttcattcttc tagaatatac ttctacgcca
    21961 aaaactgtta aaaaactttt actttagaaa attggagaaa ttgatcgctt aagataaatt
    22021 caggacacta aatagtacag gaagtagact aaaactttta aaagttttaa gtctatctat
    22081 ttggcaagtt taaaaaataa aaattcggta tcaaaaaact gttaccgata acatttttga
    22141 aagcgttgtc ttttgcttct ctatcactta tagtgatgac gtcatattaa aacacaagga
    22201 ggaaatcaat ggatacagct gcaatttatc atcgaccgga aagtgaatat gcatttctat
    22261 atacgaatac aagatttcgt attcggcttc gtactaggaa aaatgatatc aaaaaagtat
    22321 atgtgctatg tggagatcca tataccataa caactgaaaa atggtatcaa aaacaacgac
    22381 caatgaaaaa atgtctaagt accaatgttc atgattattg ggaaattgaa gtgacgagcg
    22441 aaacaagtcg cttacagtat gcgttccatg ttgttgggga agataacacg gactgttttt
    22501 acggtgatca aggaattttt ccttatcgag aaaatgtgat tactgaacca aatttttatt
    22561 ttcgaatacc ttattttcat caaatcgatc gattcactat tccagaatgg gtaaaagaaa
    22621 cagtatggta tcaaattttt cccgaaagat ttgcaaacgg ggacaaagca aatgaccccg
    22681 aacacacttt accttgggga agtaaagatc cagaccgaga agactttttc gggggagatt
    22741 tgcaaggtgt gatggatcat ttggattact tgacggacct tggcatcaat ggcatctatt
    22801 tttgtcccat atttaaagcc acatccaatc ataaatacga tacgattgat tattatgagg
    22861 tggatcccgc ttttggcgat aaaaaacttt taaaaaactt gattgatgaa gcacataaaa
    22921 gagggatacg tatcatgttc gatgctgtat tcaatcatat gggtgtccat tcaccacagt
    22981 ggcaagacgt tctcaaaaac ggagaaaaat cgatctataa agactggttc cacatccgtt
    23041 ttttcccagt agattcttat cagatgactg acttaccaga aactgctgaa aatatcccat
    23101 atgatacttt tgcttttact ccattcatgc cgaaactaaa tacagcaaat ccagaagtgc
    23161 aaaaatattt gctggatatc gcaacttatt ggataaaaga attcgatatt gatggatggc
    23221 gtcttgatgt tgctaatgaa gtcgatcatc atttttggaa aaaattcaga gaagcagtta
    23281 cagaaatcaa accagacatc tatatccttg gagagatttg gcattcctct caagcttggc
    23341 tgcaagggga tgaattccat gcagtgatga attatgcatt tacagattcg attaaagatt
    23401 attttgcaaa aaagaaaatc acagcttcac aaatggtcag tggaatgaat catcagcaaa
    23461 tgctttacag agatcaggtg aacgaaggaa cattcaactt attagattct catgatactg
    23521 ccaggatctt gaccctttgc caaggtaaca aagaattgat gaagtctgtg atggccttta
    23581 tgtttcttca aaaagggtct ccttgtatct attacgggac agaaataggg atgactggtg
    23641 aagatgaccc ggattgccgc aaatgtatga tatgggagaa ggaggaacaa gatctagagc
    23701 tattcggttt tataaaagaa cttgtttctc taaggaaaca gttgtctaag attatttctg
    23761 aaggttctac tcagtggcta atagtaaatg atcgtgaaga taagctctat ttcacacgga
    23821 aactagaggg acagatcatc tatgtttact ttaatcagtc aaaagaacct tgggtagtag
    23881 aacaggaaaa tgaggtgatt cttagtcaaa attgtcagtt gttagaagac gggaaagcag
    23941 agatacgaca atacggattt ttgatcatgt gttgaaaact gttaccggta acaacaagaa
    24001 agcgtttgca aatgaagatt tgtgcccatt ataataaggg tgtaacagca cgttgaatac
    24061 aaaggaggaa atgaagatga aaaaaagaag tgtgaaacag ttgggtttag ggttaatctc
    24121 tttaggaatc ttaggcggat tagcggcttg tggcaatggt aactcttcag cagaagggga
    24181 agccgatgaa ggaaaaacgt taacagtttc cgttgattct ggatataaag attatataaa
    24241 cgaaataaaa aacactttcg aaaaagaaaa cgatgtgaaa atcaagctag tagaaaagga
    24301 catgttcgat cagttggaat cattagcgtt agatgggcct gctggaaagg gacctgacgt
    24361 tatgatggca gcatatgacc ggatcggtgc gttaggacag caaggacatc tggcggaagt
    24421 aaaactggga aatgaagcag cttacgatga aacagacaaa gcccaagtga cttatgatgg
    24481 aaaaatctac ggagaacctg cagtaatcga gacccttgtt ttgtactata acaaagattt
    24541 agtcgataca gcaccatcaa cgtttaaaga tttggagaat ctatccaaag acagtcgttt
    24601 tgcttttgag tcagaagcag gaaaaaatac aggattttta gctaaatgga ctgactttta
    24661 ttattcctat ggtttaattg caggttacgg tggctatgta tttggtgatg atggcacaaa
    24721 tccttcagat atcggtttga ataatgcagg tgctgtagaa ggaatctctt atgcaacaga
    24781 ttggttcaaa aatgtttggc caaagggaat gcaagacata aaaagtgcag gagacttcgc
    24841 cagccagcaa ttcatgtcca ataaaactgc agcaatcatt gacggtccat ggcaagcgca
    24901 aacatacaaa gaaaacaata tcaattatgg tgtagccaaa attcctactc taaataatgg
    24961 acagccttat caaccgtttg gcggaggaaa aggatgggta gtcagcaatt atgctaaaaa
    25021 caaagacctc tctcaaaaat ggcttgatta tgtaacgaat caggaaaatc aagaaaaatt
    25081 ctttgaaatg gtaaatgaaa tccctgcaaa ccaacaagct agggaaacag caaaggctaa
    25141 aaacgatgaa ttaacaacag ctgtgattga acaatatgaa acagctcaag cgatgccaaa
    25201 catcccagaa atgggcgaag tttggacagg tgcagaaaac ttgatgttcg atgcagcatc
    25261 aggttcaaaa acaccaaaag aatccgcaga tgaagcagta aaaacaatca gtgaagccat
    25321 agaacaaaaa tactgattaa aatgataacc gtccattttt ataaaaaatg gaacaggtgc
    25381 ttcagataaa agtggcaaga gcgagatgga aaattatctt gctcttgttg ccattatctt
    25441 aaaagcagtt tgcggtttag caagtttaga gggggcattc agatgcagca agataaaaca
    25501 agcaatatca acgaaaaaaa aatcacgctt tgttcactta ttccagggat ggggcagttt
    25561 gtaaatggcc aagcttttaa aggtatcgtt tttttcgcca tattcgtcct attcattggt
    25621 cagatgcttc tgggcggatt tcatgcattg gtaggattgg ttactttggg aagtatacca
    25681 atggaagatc attcattatt tattttgatt gaaggcactt tgcaattcat cattacaatc
    25741 attttcttga ttttttacgg tttgaatctt ttagacgctt atcgagtagc gaagatgcgt
    25801 caagagggca aaaaaatcaa tcggacgatg aaggaagtca tacataatat aggtgatgaa
    25861 ggttttcctt atctattgac acttcccgct tatcttctca tgatttttac gatcattttc
    25921 ccagtcctag ttacattgtt cactgcattt actaattatg acttcaatca tattccacca
    25981 gccagtttaa ttgattgggt aggggctaaa aactttttta gtatcttctt tttaagctca
    26041 tatcgtaata ctttttttgc agtcttttcg tggaccgtga tttggacttt atgtgcaaca
    26101 actttgcaaa tcacccttgg tattctcaca gcggttgttg ctcaccagaa atttattaaa
    26161 ttcaaacgta ttttcggtat cattttctta ttaccatggg cggtcccagc ttttattact
    26221 atcatgagtt tttcgaatat attcaacgat agcgtaggtg ccatcaatac tcaagtgatt
    26281 cctttcataa accagcttcc ttttgtagat atcagtgcaa tctcttggaa aacggacccg
    26341 aactggacga aagtagcaat tatcttagtg cagggctggt taggatttcc ctatatctat
    26401 gtgatggtta caggaatctt acaatcgatt tcagaagact tatatgaagc agcaaaaatc
    26461 gacggagcaa atgcatggca gcgcttttca aacatcacgc ttcctgctgt cttcctagtg
    26521 gcagcgccaa catttattac acagtatacg gggaatttca ataatttctc catgatctat
    26581 ctgtttaaca atggcggtcc cggaagcgta ggcggaggag ctggttctac tgatattctt
    26641 atttcctgga tctataaact tacgactgga acttctccgc agtattctat ggcttcagca
    26701 atcacgttga ttatctccgt catcgttatc tgtgtgtcat tgctggtatt taaaaagaca
    26761 aatgcgttca atatggggga ttaacaatga aaaaatttca ttcgagtgct cgattccaaa
    26821 aacgactttc tcactttttt acttactttt ttcttttaat actgtcagtc attatcatct
    26881 atccgctttt gatcacagca agtactgcct tcaagtcagg aaatattgca gcttttagtt
    26941 tagatctttc aagtgactgg actttaggaa actttactcg gctattcagt gaaacacttt
    27001 acgggacttg gtataaaaat acattgatca tttctgtcct aacaatgatc gtacaagtga
    27061 ctgtagtaac tttggcgggc tatacttata gtcgctatcg ttttatcggc cgcaaatcaa
    27121 gtttgatgtt ctttttagtg attcagatgg taccgactat ggcagcattg actgcctttt
    27181 atgtcatggc tttcttatta gaagcgctcg ataagtactg gttcttgaca atgatctata
    27241 taggcggtgg aattccaatg aatacttggc tgatgaaagg atactttgac actgtaccaa
    27301 tagatttaga tgaatctgct aaacttgatg gggcagggca tttcaggatt tttttccaga
    27361 ttgtattacc gcttgtcaaa ccgatgctcg ccgttcaagc attatgggct tttatgggac
    27421 cttttgggga tttcatgtta gctaagttcc tgcttcgaac acctgaaaat cagactgttg
    27481 ctgtcggctt acaaacattt atttctgatg tgacaaacca aagagtctct ctttttgctg
    27541 ctggggcaat tttgattgca ctaccgattt gtctattatt cttcttttta caaaagaact
    27601 ttgttaccgg cctgacatct ggcggaacaa aaggatagga gcagagtatc atgcaaacat
    27661 ttccgttaaa ctatttttcc gcattacgta cgccaactca agtgtttgcg ggacgcaaac
    27721 tattatcatg gcctaaattt ttcttgatat ttgtattttt agtttccttg atggtcatgc
    27781 ctgtgactct tttttatgcc aatcagatcc aagcgattcc attagaacaa tttctatccg
    27841 ttcattcgct gatcgatgaa caaggaaccc aaaaattttc tgaacttgaa ttatctgaaa
    27901 ctgggttaca agcatcgcaa caaacgatag ctgtgacccc tgaaattttg gtaggtgtgt
    27961 ctttatctga gaaacaacaa tccgatcatg gaacttttat tgattttgag aaggaacaat
    28021 gggtgatcca acaaaaggat aaatctggta tacgccgcta tacgatgaat tatagcccat
    28081 ctttccaacc agattctgta cgtacgcccg aagatttcca acgattttta gaaagagaat
    28141 tttatgccag taatcgtcca acaatcattt tatcttatag tttaagtctt ggtcttgtcc
    28201 tatttgtcat gacgagtctc attttattcg gggcttcctt tttcttgtgg atgacaagaa
    28261 aaagccaact ttcatccatt catacattca aagagagtgc aaatttgatg ctgaatgtta
    28321 tgggaatagg ttccatggca gcagcagttg tagggtttat ccactttgac tttatcttga
    28381 tgctaagtgt ccaaacaacg ataactgtct tgctgctttt atgggttttt gtcaaaacaa
    28441 gattcaaaga taagcgtgtc gaatgacaag tttaagcgat ttatgcgaca atgaatgtga
    28501 aaaaaatgaa aaataaagta gtttttgaaa aatttctgaa aaaattaaac ttcttagttg
    28561 attcttttct tttttagtat taacgtaata gggtagtttt tttaaggagg aaaatcatgt
    28621 tagagaaaac tttatacaat caattatttt cacgttcttt ctcgctccct gtcgaagtaa
    28681 attattggga cggtacaacg aaacaatacg gagatacgga cactcctacc caaatcaaaa
    28741 tcaatatcca tgaagaaatc ccaatgaaag agattacaag taacgcatct ttggcactag
    28801 gagaagccta tatggatcat cgaatcgaga tcgagggaag tatccaagca ttgatcaacg
    28861 atgcttatca gcaagcaaac agctttttac gaggaaaaga ttatctaaaa tggctgccaa
    28921 agaaagaaaa acatacgaaa caaaaaaaca aagaagatat ccatgcgcat tatgacttag
    28981 gtaacgactt ttataaactt tggctagatc caactttgac ttattcttgt gcttatttca
    29041 atacacctga agatacgttg gaacaagcac aaatcaataa agtccatcat atcttagaca
    29101 aactctttat caaagaaggc gaaacgttgc ttgatatcgg ttgtggctgg ggaacattga
    29161 tgttcactgc taccaaagag tataatgtca aggcaactgg gatcacacta agtgaagaac
    29221 aatacgattt gattaccaaa agaataaaag aagaacattt agaagataaa tgccgtgtat
    29281 tattaatgga ttaccgcgaa ctaaaaggcg aaacgtttga tcatatcaca agtgtaggta
    29341 tgttcgaaca tgtaggttct gaaaatcttg aaggttattt caaagtggta aaagatttat
    29401 tgaaaccaaa aggaacagct ttgatccatg gaatcagccg acaacaaggc ggtgcgacaa
    29461 atgcttggat caataaatat attttccctg gcggttatgt ccctggtgtt actgaattag
    29521 ttgataatat tacaaaaaac gatttgcaac tgattgattt ggaaagctta cgtcgtgact
    29581 accaattgac attagaacat tggacaaaga attttcatca agtccaagat caagtcacga
    29641 aagaaatggg tgattccttc tatcgaatgt gggatctata tttacaagct tgtgcagctt
    29701 catttgaatc aagtaatatc gatgtgattc aatatttgct tgttcaacct ggaaacaatg
    29761 acatcccaat gcatcgtgca taattgaggc aaaagtactg tttaaagact atttaaaaag
    29821 aagctaagtt ttatgatgct ccagaagtga acttttttgt ctaacttcta ggattgtctc
    29881 taaagcttag tttctttttc tttatatttt cataataaaa gtttggttct ttaattttta
    29941 aacatgacag aaaatttttt cttcgttata cttgtaatag aatactattt tataacaaag
    30001 gggatcgaat caatgaaaaa aactgttatt tatactagtt tatttgcagc tttgttttta
    30061 gctgcttgtc aatcaaatac caatcaaggc acatctggga atacgtcttc cagtagcgaa
    30121 cagcgctctt cacaggtatc ggactcctca acaaaagcaa gcacatcttc gtctagccag
    30181 acaaaaagtt ctagtgaagc aagtactcag tcagatatca aagtgtctgc agctcgtgcg
    30241 atcgaattgt tccaagataa atacccagat actgcaatta ctagtttgga attagatagt
    30301 gattggggat cttacttcta caagatagaa ggtgtcgatg atcaaaaaga atacgaagca
    30361 agaattgatg ctcaaaatga aaaaatggag acagagaatc cagaaactct agatcgagat
    30421 gaacaaaatg gtcaaaagaa aaaagaagat ggtctggatg ttgataaatt gatcacgatt
    30481 gaggaagcag caaaaatcgc cgtcgacaaa gtaggaagcg gtacggcaac agattgggat
    30541 ttggataaag atttaggtac tacttattgg gaagtcaaag taaaagacgg caatcaaagt
    30601 acatcagtca agatcaatag ccaaacaggc gaagtactgt caactgaaca agatgattaa
    30661 gaatttcttc ggaaaacaag agttgatttt tcttttagtc atgatacact agacaaaata
    30721 acttgtttga aggagcgatt tacttgagtt tagaaacgac ctttgccaat catatctttg
    30781 ccaatccttt gatgaatgca tctggtgtcc actgcatgac tacccaggaa ttagatgaac
    30841 tggctcattc agaagcagga gcatttatca ctaagagctg tacgatcaat gaacgaaaag
    30901 gaaatcccga accacgctat tttgatgttc ctttagggag tatcaattct atgggacttc
    30961 caaacttagg tttttcttat tatctagagt atgctcttgc gtatgaaaaa gtgcaggaaa
    31021 accaaaatca accgttattt ttttccattg ccgggatgag tgtccaggaa aacttggaaa
    31081 tgcttgagaa aatagaaaaa agtgggttta atgggatcac tgaattaaat ttatcttgtc
    31141 ccaatgtgcc tggaaaacca caactagctt acgattttga ggccacttat gaaacactga
    31201 aagaagtatt cagcatcttt tctaagccgc taggaatcaa attgcctcct tattttgatt
    31261 ttgctcattt tgatcagatg gctgatatct tgaatcagtt tccattgacc tatgtcaatg
    31321 ctatcaacag tgtgggcaat ggtttgtata tcgatacaga acaagaagca gtagtcatca
    31381 aaccaaaaga agggttcggc ggcattggcg gagagtatat caagccgact gctttggcga
    31441 atgttcgcgc tttttataca cgtttgaaac cagaaatcca aatcattggt acaggtggga
    31501 ttcggacagg acaagatgca tttgaacatc tgctatgtgg cgcaagcatg ttacagattg
    31561 gtaccgagct tcataaagaa gggccggaaa ttttttctag aatcataaaa gaattaacgc
    31621 agattatgtc agaaaaagga tatacgtcaa ttgacgaatt taaaggcaaa ttacgcacca
    31681 tttcatagca aagagacgaa ggtcatgatt gatcttcgtc tttttttata aaaaacactc
    31741 gtctgttcat tcctgtgcaa gcgtactcat tgtatgttag acgaacttgt gggaaaataa
    31801 cagaggcaaa aaggaggata atatgaatga tgcaacagaa ttagcaaaag agcttaaaga
    31861 aaaagaattt agttttcaag aactagttac ggatatacag aaaaaaataa agaagcaaaa
    31921 tcatgaattg aatgcgtttg tcgcgcttga accattcgag agtaattccc acgagctatt
    31981 gattccccag tcagaaaaag ggccattggc tggtatccca tttcctttaa aaatgctcgg
    32041 tcaagaaaaa aagggatggc tagctacttc aggatctcgt ttatttgaaa cacaccgagc
    32101 ttctcataca tctaattatg tgcggcaagc agaagcgata ggtctagttc cttttggaca
    32161 aaccaatgcg ccagagtttg gtttcaaaaa tattacagac ccagtcatct atggcccagc
    32221 aagaaaccct tggaaccttg aatgtacacc gggcggatca agtggaggag ctgctgctgc
    32281 agtttcatca ggaatcgttc ctttagctgg tgctagtgat ggcggtggtt ctattagaat
    32341 acccgcttcg ttttgcggat tgattgggct gaaaccttcg cgagggacga tgccagttgg
    32401 tccatatgct tggagaggat ggcaaggagc tgctattgat ttcggcttga cagtttctat
    32461 gagagacacg gaggctttat tttatgggat gagaagtatc cattccggtg cgccatatca
    32521 agtatcgcca gttgagtggc aaacacatcc aagaaaaaaa cggttgaaga tagctttttg
    32581 tgttgattca ccgatccact cacagattag tgaagaggca gtacttgctg taaaaaaagc
    32641 tgtagatttc cttggaaaag aagggcatga tttgacagag attccttacc cactagatgg
    32701 tcgtaaactg atcacgtctt attatcagat gaatggcgca gaaacagcag caatgatccg
    32761 ctcgattgaa acaggcatcc agcgagctgt cagaaaagaa gaacttgaac ccatttcttg
    32821 gactttgttc caatatggtc agaagctctc tgccgctact tacatcgatt ctctgcatgt
    32881 atgggatgaa gcagcaattg tcatggaaaa cctacatcaa gaatacgatc tattcctttc
    32941 accaacgact gcatttaccg cccctaaaat cagcgaagat ttgcaaagtg atgcgataag
    33001 agcacagatg gaacacatca gtgattgttc tgaagtagaa ggtttagacg ttgtttcttc
    33061 catgtttgag gagagcttga agatcacacc ttatacacag ttggctaatc tgactggaca
    33121 accagcaatc agcttgccta ctcatttgac aaaagaaaac atgcctctag gaatccaatt
    33181 tatggctgcc agaggaagag aagatttgct atttcaaatc ggctatctat ttgaagcaaa
    33241 tcatcagttc catttaccaa atccatggaa atagcagaaa caaggagaag aagaatgaag
    33301 aaaaaaagat tgtttgctct aataactagt gcctttttta ttttaagcgc ttgcggtaca
    33361 gctgccgcta atcaggaaga taacagctgg agtaatgtag aaaaaaagaa agaattagta
    33421 gtagcgactt ctggcacact gtttcccgca tcttattata atgatcaaaa tgaattagtt
    33481 ggttatgatg tagatttggc aaaagaggtg gctaaacggt taaatgtcaa aatctccttt
    33541 aaagaataca acgtggatgg acaaatcact tctgtttcaa aaagagaatc agattttgct
    33601 gcgaacgatt ttggtatcac aaaagaaaga gaaaaaaaat tctccctttc agagactttc
    33661 aaatattcat ttgacagtat gatcgtacga aaaaaagatg attcaggaat ccactcactt
    33721 gaagatctta aagggaaaaa agcagctggc gaacccaata ctagctatat gcgtttagcg
    33781 gaaaaacacg gagctaaatt agtcacatat gacaatgcca cgaatgatca gtatttaagc
    33841 gatgtagcaa atggtcgaac agatgttatt ctaaatgatt attatctgca gaaaatggcc
    33901 gtatctgctt taccagatat ccctgtaaaa atactggaga acgtttattt caatgaaaac
    33961 gaaagtggtt ttttattgaa agaaggaaat gatgctctga aaaagaaaat cgatggcgta
    34021 ttaaaagaga tgaaagaaga tgggacgaca aagaaaatat ctgaaaaata ttttcaaact
    34081 gatgtttcag tcaaaccgaa agaaacgatg gctgagtcgg tgagtgagtg atgtactttc
    34141 ctctaattga tttttccctc atgtggactt cacttccttt cgtactgcaa ggcttggtat
    34201 acacattagg gattggcttc gtgtcgtttc ttttaggaaa tctggtaggg ttgcttttga
    34261 cagttttagg tcttttggat tggctgcctc tgaatgtatt tattcgtttt tatttatcgt
    34321 tctttagagg cattcccgca ctagtcctgc tgtttctgtt atattttggg ttaccttatc
    34381 aattaagtgc cttaacggct tcagtcatct gctttacgat taccagcagt gcatttattg
    34441 gtgaaatcta tcgaggatca ctagcaggcg tttcttcagg tcagtgggac gctggtttag
    34501 cttctggttt ttctttcttt ggggtattga aatatatcgt tttgccgcaa gcttttcgaa
    34561 tctcagtacc ggcactcagt aacgtagcta tggatctagt gaaaggtact tcgttagcag
    34621 caacaatcac agtgccagaa atatttcaaa aagcaaagat cataggcggt cggacattcg
    34681 attatttttc tctctatatt ttagttgcac tgatttattg ggtcatttgt atcgggatcg
    34741 aaaaagtgca acagtattta gaaaaacgtt ctatgcatca gtcaatctga aaaatagaaa
    34801 tggtgctagc gtggctggca ccatttctat tttttgtttc ttgtatattc tgcctagtat
    34861 tttaaaacca tttcatgtaa aatgtaaatc gaaaatatgt tcggaggtag aaaacatgaa
    34921 gaccccgcta accatcgaaa tagaatcctc gttataccaa tattgcatcg agcaaggcgg
    34981 cttggttgta gaagaagtca cgatgcctga tgaccaagga attgtagata cgcttagctg
    35041 cttgacaaaa tttgatggaa gccgagaatg gcgatgctat gagctgaaag tgacaaaagc
    35101 agattttcgc tctacagcca aactgtcatt tgttggacat tataattatt ttgttttacc
    35161 aagtaaacta tatgaagaag taaaaactga aatccctaca ggaataggtg ttttagtgta
    35221 tcgtccgtac acggttgaag aggagatgcc tgcctctggt acatttctga ttgcgaaaaa
    35281 agcagcaaag aaagagcttg ctgtaccgga agaagctttg acaaaccgct tcatggcttc
    35341 tttattcagg gaagtaagaa aagcaaagca aatggagcga ggcgtaaaat attttccaac
    35401 agatcaattg tacaaagaat tgaaaagacg ttcagaaaat caagatccat tttctaacga
    35461 acgatattat gaacggtttt tagaggaaat agctagtcag gcaatcaatg agttgcaaga
    35521 agaagttatc gcgcttcagc atgattatga atatttgagg caacaacagc agatccgtcg
    35581 gttgcctaca gagccattgg agtgaaaaat atgtactatc cttatttaag aggaaaacaa
    35641 tttgacctat tagcattaaa agaagcactg tcgcgaggat tgctctccaa taaaatccaa
    35701 ccagtgatcg aaccagtacg tgattccgct acgcttaaaa atgtgatcga attatttcaa
    35761 aaaaagcacc atcgaatcgc tgtgatccaa aatccccaag taggacaatt caaattattc
    35821 gatcagcatg tgcacagttg gacagtcaaa gaaaatagct ctgtcgtccc tgcacagatc
    35881 ttaacgccag aaaatctcag tgaagtactg gattcacctc cagcgtttct tgttttcgat
    35941 ggacagcact acccaaaaga tacagaggtg tggaaacagt tggcaggagt tgattcgaaa
    36001 tttttgatac cagatactag tcgctttcgc atctggctgc ccgaaaacaa aattgtgatc
    36061 cgtgactctt ttcaaacgag aaagcatgtt gaaagttatg cagataaaac agatgacttt
    36121 tttagtgatg actatctttt tttccacgca gacggatata ttggattttc tgatttcacg
    36181 atcgaaggta gccgttattt cgataaaggg ttccctagcc gagcgcttgc tatccatcta
    36241 acgtatattg atgcttacgg caatatacgc gtgaaacatt ttgtctctga cagtaacgac
    36301 tctgctaaag accaagcatt gaagtttctt gaagcaggag ataaaatgaa aaaatggatc
    36361 atgcgtcacc atcatcaact cttgattact tcaggaatga ttgaattatt ggctctttac
    36421 cagcaacaaa aattccctgg actgggagtg ttgaaaaagt ggtcacttat gcatcatttg
    36481 gaacttatta gtcacctgct agatcatcca aaagattggc ttaaatatta ttcaaaagtt
    36541 caggaactat atgaagtgat ccaatgatta gataatgtaa atgaaaaagg aatagaaaag
    36601 aggaaactgg atgtatattc ggcaaacaag gaaagaagaa gtagaagaag tggtagaaat
    36661 cattaatgcc gcagcggatt tcattgcttc gcaaggctct cctcaatggc aaaacgggca
    36721 gaaaccaaca aaagaaacga ttttgaacga tattgaaaga aaagaaagct atgtattaat
    36781 agtggaagat caaattgctg gaacagctgc attagtctca agcgtggatc ccgtttacac
    36841 aaaaatagat ggtagttgga aagaatcaac tggtccttat ttatcgattc atcgtgtcgc
    36901 tttagcagaa agtactcgag gaaaaaaact agcaaaaccg ttcttagaac atttgttgac
    36961 gattgcagcc agcaaaaata tccaagatat ccgtatcgat acttatccag ctaatgaacc
    37021 gatggaaaag accatctatt ctgctggatt cacttatcaa gggatgattg aatttccttt
    37081 tgaacacggc gaaagaaaag catatcaaat aatcatcaat tgactataaa atcatctgag
    37141 caaagataaa gacattaaca ctactgaaaa aaacaatcat atgatagcca agaggttgta
    37201 acagaagtgg ccagcttcaa aaaacaagaa agaatttccg aaaatgcttc tcaaattttt
    37261 gtggaatttt agcgtatttt acgaaatagc tgctttctga aactcccttc atcgagaggt
    37321 cgtgacatag cttttgtcac gacctctcgg cttatcctac gagtattatt cgatacattt
    37381 atatcaataa tccttttttt attttatctg cagcttcttg gaacgctcca tcgtttgaca
    37441 cgactaaaag tgaggggata gctttcaaac gtttcaactg tgctaaatct tctacataaa
    37501 ccgccagtct ggctgaaatc tgttcagatg aatctccacg ttttttcagg cgcgattgca
    37561 atgtttcttt agagatcgta ataaagatag gacggactac cgtcccgaaa tactcatata
    37621 aatgccaaaa accatttgct gtcaaaacgg cgtaacaatt tcctactgtc agtttctttt
    37681 taatttctgc tttactggaa ccatatttat gttgagcata ttggtcccac tctgcaaact
    37741 catttccctt gatcatctga ttgaatgtat tgtcagaaac aaaataataa tcctcttgat
    37801 ctttttcccc aggtcttggt gatcgattcg tgtaagtaat gatcttttgt tccggctgaa
    37861 aaatagcttc tgcgagtgaa gtttttccag aaccagaagg accgataaat acatagcaat
    37921 aattcattgt catttctcct ttgatgattg aacaagtgaa aaagctttgg aaaatatttc
    37981 caaagctttt aaaagtaact ttgctttttt gtatattata ccagcaagcc agtcacaata
    38041 gcacttaaaa agctgactaa agtcgctgtt agcaaaattt tcaatccgtg ttttgcgaca
    38101 agatttcctt tttcttcatt taatcccttc atcgcaccag aaatgatccc gatagaagag
    38161 aagttggcaa atgaaacgag gaagacagat ataattgctg ttgtacgctc agtaaaatgg
    38221 aaattaccat ttgctaattc tgtcatagca acaaattcat ttgtaaccag tttagtcgcc
    38281 atgatactac cggcatccac tgcttctttc catgggattc cgctgatgaa ggcaagaggg
    38341 gcaaagatgt aacctaggat ctcttggaaa gtgatgccga atatcccgtg gaagatagca
    38401 ttgatcatgg caatcaaagc aacaaaaccg ataagcatag cagcaaccgt gatcgccaca
    38461 tggaaaccat caagaatgta ttctcccaac atttggaaaa atgtctgttc tttgttttct
    38521 tcaatgatca gctcatcttc tttttcttct aatacatagg gatttacaat tgaagccaga
    38581 atataaccgc caaataagtt caaaacaagg gcagtgatca catatttaga atcgatcagt
    38641 gccatataag atccgacgat cgacatagaa actgtagaca tagccgatac actcatggtg
    38701 aacaaacgtt tctcagatag gaagggtaat tcctttttta tggaaataaa aacttccgat
    38761 tgtcctaaaa tagcagaagc tactccatta taagattcca atttgcccat cccgttgatt
    38821 ttacttaatc ctagaccaac agctttcata aataatggaa gaatcttgat atagcgtaga
    38881 ataccgataa tagcagaaat aaatacaatt ggcatcaata cggaaagaaa gaaaggtgca
    38941 cttcctttat ttgctacacc ggcaaaaaca aagtcaacac cttgcccagc aaaagcaaga
    39001 agatgatcaa acatttttgc caatacagaa acaaccgctg taccaaaagt tgtacgtaat
    39061 aatacgaaac caagaacaag ctgtaaaacg aacatcacaa ttagtggttt caatttgatt
    39121 gcttttttgt tactgctgat caaaaaagca agccctaatg tgacaaataa accaataata
    39181 cctaataaat aactcaaata atcactccta ttccaaattc cgttttattt tacctgtttt
    39241 gcaaataaaa tactaatctt tagtgaaatt ttcattactt ttttagagtg aaacgcattc
    39301 attttccatt agaaaataaa gcgaacaaaa taaaaggttt aaaaagtgta acgtcttagt
    39361 aaagtctata aaaaaacgaa actatcgatt aagctaatag tttcgttttc aacatttata
    39421 agtaaacttt ttctattggt aatatagatt ttcggaaggg tagacaggtg cgcaggtaat
    39481 atcgattcct agttttttca atgtttgttc atcattttta ttgaccatca cagtagagtg
    39541 ggcttgtgct ccctcaagtt cagctagttt atcataagca acttgagcga ttggattcgt
    39601 tactgcactg attgataaag cgatcaagac ttcattgatg cttagtgcat ccaaagagga
    39661 atgaagtgtc tttgatttca atccttgtat tgtttcaaga actaatggtg acaataaagg
    39721 aatgtcatct gcgatatggg ctaaatgttt tactgcattt aagataactg ccgctgacgc
    39781 atccatcaat gaacttgtac gcccggtaat gatgacttca tctggtaatt cgattgcaat
    39841 cacagcagaa ggatcttttg taggactttg cgcttgaatc ttatctgcat agtcatgggc
    39901 agggactacc acgtttcggt cttcaggacg taaaccgact tcttccataa tcagtttgat
    39961 tcgattaact gcttcttcgt cgatcaatcc ttttttgaag tcgcattctg tttggaaata
    40021 acgacggatg atttcttgtt tagaggcttc ttggacaacg gtatcatcca cgatcccaaa
    40081 tcctacacga ttcacaccca tgtcagtagg agattgataa acagattctt taccagtaat
    40141 tttttcaata atacgtttga ttacaggaaa tgtctcaaca tcccgattat agttcacagc
    40201 tactttattg taggcatcaa agtggaagga atcgatcata ttgacatctt ttagatcgac
    40261 tgtggctgct tcataagcaa tgttcagcgg atgtttcaac ggaacattcc atactgggaa
    40321 agtttcgaac ttggagtaac cagctgcatg tcctttttgg ctttcatgat acagttggtt
    40381 caaggaagta gcgagctttc cacttcctgg gccaggagct gtcactacaa cgattggttt
    40441 agttgtttca atatactcat tttttccaaa accatcttct gagacgattt tttctacgtt
    40501 gactggatag tcttcgattt ctgagtgttt atagaccttg atgtttcgtc tttctagttt
    40561 attgataaag actttcgtgg caggctgtcc actatagcga gtgataacga cactgttgat
    40621 taataagcca taaccacgta attcatcgat taaccgcaag atatccatat cataagtaat
    40681 cccataatca ccacggatct tattacgttc aatatctcca gcatagacgc aaatcagtat
    40741 ttcagcttga tctttcaatt tttgcagcaa tttgattttc gcatcttctt caaatcctgg
    40801 aagtacacgt ttcgcatgct tgtcgtcaac tagcttccca ccaaattcta aatataattt
    40861 gtcatagtgg ttcactcgtt ccaaaatata cgctgactgc tcttcaatat atttttctga
    40921 atcaaaacca atttttttca ctaggttccc tccttaagta ctaaagcatt ttttcatttt
    40981 tttgaggatt cgtcaactgg aagagcgaaa ttcctcaaaa aaacaaagaa aatggtttaa
    41041 accaacttgt ctagatagtt attcattggt atgatagttt ttgataagga aggtgctaca
    41101 tgaaaaaaag acagaaaaag aaaaacgcat ataaacacta tatccgttcg atttttaccg
    41161 gttatgaaag aatgcttgaa gatcccgaat tggaacaatt gacgtttact tatctaaacg
    41221 aagaaacgca gctaactcgt gatgagcatc agcgcattca tttcacaaca agagatctgc
    41281 ctagtaaata aaaaataaga taaaggtcat cggattcgat tctgtcctat cagatggaaa
    41341 gactagttga tcgaaaaaaa ggctgtacca aattttggtg cagcctgttt tatggagcat
    41401 tgcccgctct tttttataac aattttttac agaaaatggt actattgaag tattaataag
    41461 tgaaaaacag atgagtaaga ggaggcaagt agtatgaaag agccagatac aaatgcaaat
    41521 tatgagccag acgaactaga ggagacgaat gcggatcagg atacaggaca agaatcgtct
    41581 tctttttacg atatcaaaga aaaagcaagc atgaatcttt cccaattatt agaagactta
    41641 caggcttttg aaaaagctgt ccgcgaagaa gatatccctg agatctaccg tttatacaat
    41701 ggccgattga atgaagaatt aaaaagaagc tcaaatgaaa atcacgagat cgatcaattg
    41761 ctgatgcgta agatccatga tcgtttttta caggaatttc cttttatgga acaagtagaa
    41821 aacatctcgc caacgatgag ctattacaaa atcggcactt attaccatga tcggcccacg
    41881 gtcggtattg atgcaagtct gccagaaatt tttgttttgc ctaagatcga tgaagagtgg
    41941 gagaaatatt cccaaccgga gtcaacggac tatgacaaaa aaatcaatga gctagacgca
    42001 aaggtgatca ctgctcagac agaaattgaa cgtttagatg aacaaataaa agaaatcaat
    42061 cgacaaatga ctgatttgaa cgacaataaa ggattcttga atcgtaaaaa aatcgaggaa
    42121 gaaattcaag aattagagaa gaagaagcaa gtactatcta atgaaaaatt aggctggctt
    42181 ccttatattg agacgcctga aacaatccag cagcaaaaag aagcattgaa gcaagaagca
    42241 agagcagacc agcttcgtgc agccatcgta gaaaaagaac aaaggcaaat cagacgctat
    42301 ttcggtagca aagagggatt cggtcaagca atccatgaat tcttgatgaa ttatcttggc
    42361 aatgagaatc ctaataatca atctaatgaa ggaggttccg agtatgaatg aacacgatga
    42421 agcagaatgg acaaaaaatg aagaacaaac agaagatcat gaagaaacga acacttcatt
    42481 ggaagaacaa acaagtaatc ctctggatga cacagaagaa atccctatgc tagaggaaac
    42541 aaaaatacac gaaaaagaaa aagtaactcc gacgaatacg aatactgcag aaaacaccca
    42601 tgcttacgaa ataactgaaa atcccgggta tttagaagga aaagagcaag atcatcaaga
    42661 tatcgtcagt ttgattgaaa cagcggaagc agaattagcg gatttacgta tgagaaaaga
    42721 tttgatcgaa tcagattttc cacaattatt agaatcatat gatcgtttta tattgaatga
    42781 agaaccaatc tccacgatcg gaaagacaag cctgatcgaa tacttcacgt atgaattact
    42841 gaaaccgttg aatgatattg gtctggaacg gtcaaattca gcttatgaca catggaaaac
    42901 aagacatttt tcgatcaatt atacgttgaa tggacaaaac gaattagtct tttcctttgt
    42961 attaccgacc atcgatcaac gataccaagt ggaatcgatg agtctcctaa acgtatcacc
    43021 agagacaatg gaaatcaatg tccaagatga ccgggtgctt tctttgatcc gacactggtc
    43081 agtggatcga gtattctctg cagggcagat cacgatattc aatcacaaat tgaatcaatt
    43141 attagcccat gcaagaacac ttggttttac cgttaatcag acattactag acaatacgaa
    43201 gccacttcgt ttgaatcttc agagcgaatt tgaattgacg aatgaagtat tggatgatat
    43261 ttttatcgtg gcaatgaaaa atccatccta cgatatggaa aagatcaacg aaactactta
    43321 tcaagtacga ttagataaag gacagtcttt aacgataaca acaaacgaaa aagatcaaac
    43381 agtcttgtcg atctcatctg gagactaccc acgatctgtc attgatttct ttatcaatta
    43441 cgaattcttg gtcccattaa tggtgagaaa gtcctgattg gataagttgg attaccagat
    43501 gtcattcaga taattctgtt ccaaaggact tgcggcagtt atccagaata attgtcacaa
    43561 gtcttttctg tatgtactaa atggacgtga ataacttgct gatgaaggag ggatttcatt
    43621 aggaatgtcc acaaataaaa tagcacagct tagcttattg tctgctgctt gtgtagcagg
    43681 taggatcgct tttcaattta ttccgaattt ccagccaatg actgctattt ttttatttat
    43741 agtactttac ttaagcttaa aagatgcgtt aattgtcatg agtctttcta ttgcgatcag
    43801 cagtttttat tttggtgttg gaccatgggt catcggccaa tggatcagct atactgttgt
    43861 tctgagtttg ttttcaatcg tctgcttgag aaaaacagtg caaaggaatc tatggttaaa
    43921 gggagtctgt ttttttctgg cgggcatggt ttacggaata ggtatgaccg tttttgatac
    43981 gctgttatat cgtttgcctc aaccttggat ctattacctt caaggggtgt cgtttgattt
    44041 gatgcacagt ataggcaatg ttgtgttctt tcttgttttt ctgccagtcg tcaaacgttt
    44101 ttacaatcat tcaggaggaa aaaatgaaaa aaataattta tagtttgctt gcaatgagtg
    44161 tcagtatact cattttgaca agttgcagta caacctatac acaatcggct tccaaccaaa
    44221 aggaggaagt aaaaacatcg caaaaagcaa caatcacgtt acaagaaaat ggtcaaaact
    44281 tttcggaaaa agaagtgttc ttcaaaaaag gcgatgattt actgagtatt ttgaaacgaa
    44341 attttgaagt caaagaagat aacggcttca tcacttcgct tgaagggcac tcacaggatg
    44401 agaaaaatca actctattgg atgtttactg tggacgggaa aagtgctact actggtgcca
    44461 aagagatcaa attgaatgat ggacaatcgg tcattttcaa tttgtctaaa ctataagttt
    44521 cataaaaaac tgttaaaggg cgaatattgc cataattcag tgagttcagt tgatgaaaga
    44581 aggaaaataa catggaaaat tttaaaaaaa gcggaatgtt ttccgtaatt gctgctctag
    44641 gggctaatat tctagttgct atcagcaaat ttgtcggcta tgcaatcagt ggcagtgccg
    44701 ccatgctaaa cgaaagcatc cacagtatcg ttgattgcag caatcagatt ttgctgttat
    44761 tcggtgataa acaagccaat aaaggacaga gcgagttgca tcaatttggt gaaggacgag
    44821 caaaatactt tttcagtaca atcgtcgcaa tgatgctttt cttcggcggt ggggcacttg
    44881 gggtgatgga agcttttgaa aagttgacgc atccatccca tgaagtgggt aatccgatca
    44941 ttgttatagc aatactgtta tttggtttgg taatcgaagg aagttcgtta cgcgtggcaa
    45001 tgaaagaaat caaagaattg aatactgaaa aactttccac gatgcgcttt ttgcgcgaaa
    45061 gtcgacacag tgaaatactg attattttta cggaagactt gtgtgctgta atcggtttgg
    45121 tcttggcttt agcaggaaca atattgactt atgcaaccgg gattgctgca tttgatgcga
    45181 tcagcggatt gctgatcggt ttcttgctga tgggagccgc cattttccta gcaaaagaat
    45241 tttatagtct gatcatcggt gaaagcgtga cagcaagcga tttatcaaaa atcaagttag
    45301 cttttgaacg tcctgagatc agtcggctga tcgatataaa aacagttcat ttaagtccaa
    45361 cggagatttt agttgcagca aaagcagata tcgttggtaa caaagagaat gaagcttact
    45421 cggtcatcaa tgatattgaa aaaaatatgc gtcagtctct tcctgacaag aagatgtata
    45481 tatacattga aacagataaa tatgacccaa attattctcg taaataaaac gtttattcat
    45541 gaattgaagc aaaaatgaga aaaatataat cttgttttta aataaaacgg ctacaattta
    45601 gcgaatttat gttacaatga gtccgctgtt tatttgttgt aattaaagga gatattttaa
    45661 tgaacaaaaa gaaaagtgcg ccatggcttc aagctttgca tgcagtgatg ccactttgta
    45721 taagctatat accagtagga ttagcttgcg gagtattgtt gcaaaaagtt ggatttgatc
    45781 ctctcttatc aggactgtta tcgattctgg tcttttccgg tggcgcgcag tttttagttg
    45841 catctatgct tacgacgcaa gcatcatttg caacgacatt attgatggtt ttctttttag
    45901 aattacgcta tacattatta ggagcaagtc ttgccggttt tatgaaaaaa gaaaagcgga
    45961 cgtttttggc tgtcttttct caatcactga atgatgaaaa ctatgcggtg aactacttaa
    46021 agttttcaac tgacccttcc tggaataaaa gaaaggcttt atatgtcaat tggttctcaa
    46081 tgactgcatg ggccggtagc aatatgatcg gtaatttttt cggctcggtg attcgagtag
    46141 atgctgacct tgtccatttt gctttgacgg ccatgttcat tttcatgttt atcatgcaga
    46201 tgaaaagcgc actactgatc tttacaggtt tattctcagg cgtattaggg gtcgtattca
    46261 tggtattatt ccaaaatact ttcggattga ttgcagcaac agtcattgct tcatttgccg
    46321 gatttatgct ggagaaggca ttcaaaaaag aaaaagctaa gaaactaaga gaccgcggta
    46381 aagatgtcaa tgagccgctc ttcaacttcc cggaacagga ggccaatcac catgactaaa
    46441 ctatatttgc tacttttagt tgcttgtctg ttcatcgtgg catatatccc gcgcttgttc
    46501 ccgatgcttt attttactca tcgaaaagtg ccatcctggt tcagtgactg gatgaaatat
    46561 gtaccggtgg cattatttgc ggcgttagct tttaaagatg ttttcattac tcatgaacat
    46621 ctggatattg cttggaatat caaaatagct gcaatgattc tagtagcagg cgtggcttac
    46681 aaaacacgtt cgatggcgtt gtcagttttg acaggacttg cgtctgtctt tttgctgtct
    46741 atgctttaaa ctgttttatt ttattaaaaa cagagattgc gacataagtt gttcaaactg
    46801 atctatccga actgcatttg caacagtcaa ttctggatgc aagtttattc gtagggataa
    46861 aaaagattag tgttggctta tgatgcaagc tcttttttat tttaggaatt agagagggat
    46921 ttgtccttct atgatttttt gctataatag ggaagatgtt tattttatga aagaatagtt
    46981 gagggaattt aaatgaataa aaaattgatg caaggaacat tttggctaac ctttgctaat
    47041 cttctatgta aagttttagg ggtagtctat ttgattccct ggctaagtat gatggggaac
    47101 aatcaagatg gtatgcttgc tacgacattg tataatgttg gctatcttcc ttatggattg
    47161 ttcctgatgc ttggtacagt cggatttcca aatgcgattg caaaaaaagt agctgtagcc
    47221 acaaaagaag gagataacgc tgcttgtcgg ctgattttta gaagtacgat caatatcatg
    47281 tttgtcatcg gtatcgcctc tgctgcactg atgtatttat ttgctccttt actagccgga
    47341 atcagtccga ttgctaacgt taataatggg atactagcta tcagaagttt atgcccatct
    47401 ttgattgcta tcccaattct tagtgcaatg agaggatatt tccaaggaaa aaatgatctg
    47461 cggccttatg gaacttcttt gattatcgaa caggctgtgc gagtgatcgt tatcttggct
    47521 ggtacttatt acttgcgcgt attgactgac ggaaccatat tagaagctgt attgatcagt
    47581 acagtcgctt ctttcttcgg tgggttagct gcgattatcc atatgtatat cgtcggacgc
    47641 agaaaagact tcttcgaact taaagatttt ttgatttcca gccgttattt cgaaagagaa
    47701 aaccgatctg cctctgtctc tatcattcgg gaaacactac cgtttatttt tgtcggctca
    47761 gtcattacta tcgttcaaat gatcgaccaa gtaacgatga aaccactgct tcactttttc
    47821 cgtccagaga tcgcagatca gcaactggaa ttcttattca gccgtgcttc tgtcaatcca
    47881 aataaattga cattgatcct gatctccatg gtaggaacag tagctataag cagcttgcca
    47941 atattaagca cgatgaagaa aaaagatcgg atacaaatcg aaaaaacagt aggagatagt
    48001 ttttcgattg cgttattgat tcttcttcct tcattgactg gaatgtcttt attagctgga
    48061 ccactataca cattgttttt tggctatgat cctgaaagtg tcggatattt ccaaatggct
    48121 ttgctagctt ctttgttttt ctctttattc acgatcctat ctacgatgct tcaatctttg
    48181 aatcaccatc gttttgcgat ccgattaact gtagaagcaa ttatcctgaa agtcatcttc
    48241 caagtaatcg gattaggatt agttggcgga tacgggatga gcttatcaaa tggcttagca
    48301 ttcggtattg tctttgttcg cggctatcac tttatgtgca aagaataccg aatagcacct
    48361 ttagcaaaga tcagcaattt tttcttaaaa actttccgct ccaccctgat tatgcttgca
    48421 gtatgttttg ccgttttctt tttgctgaat cagcgattgt caatggaatc aaaaagctat
    48481 gcaatgattt attgtgtggc tgtaggaagt attggcggct tagtattttt attttctcaa
    48541 ttcggaaaaa atgggtttcg aatgttgaaa aatgttaaac atcatagcag aagcaaagaa
    48601 taggttaact aatcgttttt tgctgtcact tgaaaaacgg caactcgtat gattacgctt
    48661 gtcgtttttc tattttccaa ttttagtttc atatctaatc ttacttatga tatagttata
    48721 aggtctggaa aaattctttg aaacgaggaa attaaacaca tgaaacttgc agtagttaca
    48781 gatagttcgg catatctgcc ggaacgtatc aagaaccatc ccgacttatt cgttattcca
    48841 atccctttga ttatggatgg gaaaatttat aacgaaggaa tcgatatcga agcggatgaa
    48901 tattatggac ttttaaaaaa cagtaaagat tttcctacta cttcacaacc tgtcgtaggg
    48961 gaagttttgg gtttatatga ggaattgaaa gagaaagggt atgacacgat tttaagtatc
    49021 catctttctt cagggatttc tggctttgtc aacacattat ttgcgatgaa agatgacatc
    49081 agcggagtaa cggtcattcc ttatgactct aagatcacta gtatgccgat gggacacatg
    49141 gtagaagctg ccttagactt aaatgatgaa ggtcagtccc ttgatgaaat acttgcacat
    49201 atcgatcgaa tccgagataa tacctatgct tatctgattg tagatgattt gaacaattta
    49261 gtacgaggag gacgtttgac aaatggtgca gcgctgattg gcagcttatt aaaaatcaaa
    49321 ccgatcctga cattttctga agggaaaatt gttttattcg aaaaaatccg ttcaagtaaa
    49381 aaagcatttg ctcgagctga agaaattatt ggtagaagaa ataacgaaat acaaccgcca
    49441 gtcaaacttt acgtgataca tgcaaataat ttagaagtag cgattgcgga gaaagagaaa
    49501 ttgcagaaaa aatatccaaa tgcagaaatc gaaattggtc atttcggacc agtcattggt
    49561 acacatttag gtgaaaaagc catcggcatt gccatttcag cgcaataatt ccttttattc
    49621 aaaaaacata ccaaaaatat
//
LOCUS       DACGLW010000010        45989 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.010, whole genome shotgun sequence.
ACCESSION   DACGLW010000010 DACGLW010000000
VERSION     DACGLW010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45989
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.010"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            complement(<1..60)
                     /locus_tag="IXT53_000662"
     CDS             complement(<1..60)
                     /locus_tag="IXT53_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HAQ4904514.1"
                     /translation="MYQVITMFGDNEPWWFFEDW"
     gene            complement(189..749)
                     /locus_tag="IXT53_000663"
     CDS             complement(189..749)
                     /locus_tag="IXT53_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ4904515.1"
                     /translation="MKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDIKAPSLY
                     NHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSNQAVYEL
                     LISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYLSLRFLG
                     YFTKEPTIDPEDSYFWMINNFIATLP"
     gene            899..2191
                     /locus_tag="IXT53_000664"
     CDS             899..2191
                     /locus_tag="IXT53_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ4904516.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            complement(2251..3369)
                     /locus_tag="IXT53_000665"
     CDS             complement(2251..3369)
                     /locus_tag="IXT53_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HAQ4904517.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(3383..4804)
                     /gene="arcD"
                     /locus_tag="IXT53_000666"
     CDS             complement(3383..4804)
                     /gene="arcD"
                     /locus_tag="IXT53_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HAQ4904518.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            complement(5050..6171)
                     /gene="mnmA"
                     /locus_tag="IXT53_000667"
     CDS             complement(5050..6171)
                     /gene="mnmA"
                     /locus_tag="IXT53_000667"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HAQ4904519.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            6575..7174
                     /locus_tag="IXT53_000668"
     CDS             6575..7174
                     /locus_tag="IXT53_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphatase"
                     /protein_id="HAQ4904520.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(7312..8598)
                     /gene="efmA"
                     /locus_tag="IXT53_000669"
     CDS             complement(7312..8598)
                     /gene="efmA"
                     /locus_tag="IXT53_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HAQ4904521.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(8869..9213)
                     /locus_tag="IXT53_000670"
     CDS             complement(8869..9213)
                     /locus_tag="IXT53_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HAQ4904522.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(9350..10486)
                     /locus_tag="IXT53_000671"
     CDS             complement(9350..10486)
                     /locus_tag="IXT53_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ4904523.1"
                     /translation="MKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGRHAH
                     EKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIEHPA
                     VLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLMPIK
                     EIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKRDGV
                     NIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLILDTL
                     EANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNIEPS
                     HVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            complement(10675..11649)
                     /locus_tag="IXT53_000672"
     CDS             complement(10675..11649)
                     /locus_tag="IXT53_000672"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAQ4904524.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(11729..12604)
                     /locus_tag="IXT53_000673"
     CDS             complement(11729..12604)
                     /locus_tag="IXT53_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HAQ4904525.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            12734..14641
                     /locus_tag="IXT53_000674"
     CDS             12734..14641
                     /locus_tag="IXT53_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HAQ4904526.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(15182..18460)
                     /locus_tag="IXT53_000675"
     CDS             complement(15182..18460)
                     /locus_tag="IXT53_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HAQ4904527.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(18731..19276)
                     /locus_tag="IXT53_000676"
     CDS             complement(18731..19276)
                     /locus_tag="IXT53_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ4904528.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(19288..19980)
                     /locus_tag="IXT53_000677"
     CDS             complement(19288..19980)
                     /locus_tag="IXT53_000677"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HAQ4904529.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(19999..20283)
                     /locus_tag="IXT53_000678"
     CDS             complement(19999..20283)
                     /locus_tag="IXT53_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4904530.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(20296..20859)
                     /locus_tag="IXT53_000679"
     CDS             complement(20296..20859)
                     /locus_tag="IXT53_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HAQ4904531.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            21087..21749
                     /locus_tag="IXT53_000680"
     CDS             21087..21749
                     /locus_tag="IXT53_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     protein"
                     /protein_id="HAQ4904532.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            21873..23069
                     /locus_tag="IXT53_000681"
     CDS             21873..23069
                     /locus_tag="IXT53_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HAQ4904533.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(23170..24546)
                     /locus_tag="IXT53_000682"
     CDS             complement(23170..24546)
                     /locus_tag="IXT53_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ4904534.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(24583..25449)
                     /locus_tag="IXT53_000683"
     CDS             complement(24583..25449)
                     /locus_tag="IXT53_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HAQ4904535.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKILDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(25538..26563)
                     /locus_tag="IXT53_000684"
     CDS             complement(25538..26563)
                     /locus_tag="IXT53_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ4904536.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(26581..27255)
                     /locus_tag="IXT53_000685"
     CDS             complement(26581..27255)
                     /locus_tag="IXT53_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ4904537.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(27439..28332)
                     /locus_tag="IXT53_000686"
     CDS             complement(27439..28332)
                     /locus_tag="IXT53_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ4904538.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(28406..30103)
                     /locus_tag="IXT53_000687"
     CDS             complement(28406..30103)
                     /locus_tag="IXT53_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ4904539.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(30096..31409)
                     /locus_tag="IXT53_000688"
     CDS             complement(30096..31409)
                     /locus_tag="IXT53_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ4904540.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(31409..34927)
                     /locus_tag="IXT53_000689"
     CDS             complement(31409..34927)
                     /locus_tag="IXT53_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HAQ4904541.1"
                     /translation="MDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEK
                     KEISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMV
                     NFYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETL
                     YKDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDV
                     LFRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGS
                     SNNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAI
                     GRNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVS
                     PFGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYK
                     FSPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSS
                     SIRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFET
                     KMPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEY
                     TSGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNI
                     NKLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDG
                     KYVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRL
                     YDNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTL
                     DGAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQP
                     LHLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIID
                     GVLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPND
                     SSLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTL
                     KVEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYIL
                     LAAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(35220..35678)
                     /locus_tag="IXT53_000690"
     CDS             complement(35220..35678)
                     /locus_tag="IXT53_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ4904542.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(35976..36494)
                     /locus_tag="IXT53_000691"
     CDS             complement(35976..36494)
                     /locus_tag="IXT53_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAQ4904543.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(36496..37113)
                     /locus_tag="IXT53_000692"
     CDS             complement(36496..37113)
                     /locus_tag="IXT53_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAQ4904544.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(37110..37964)
                     /locus_tag="IXT53_000693"
     CDS             complement(37110..37964)
                     /locus_tag="IXT53_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ4904545.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(37961..38872)
                     /locus_tag="IXT53_000694"
     CDS             complement(37961..38872)
                     /locus_tag="IXT53_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ4904546.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(38913..40247)
                     /locus_tag="IXT53_000695"
     CDS             complement(38913..40247)
                     /locus_tag="IXT53_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ4904547.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            40455..41285
                     /locus_tag="IXT53_000696"
     CDS             40455..41285
                     /locus_tag="IXT53_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HAQ4904548.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(41529..41778)
                     /locus_tag="IXT53_000697"
                     /pseudo
     CDS             complement(41529..41778)
                     /locus_tag="IXT53_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(41969..42163)
                     /locus_tag="IXT53_000698"
     CDS             complement(41969..42163)
                     /locus_tag="IXT53_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4904549.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(42668..42853)
                     /locus_tag="IXT53_000699"
     CDS             complement(42668..42853)
                     /locus_tag="IXT53_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4904550.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(43703..44044)
                     /locus_tag="IXT53_000700"
     CDS             complement(43703..44044)
                     /locus_tag="IXT53_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ4904551.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(44044..44274)
                     /locus_tag="IXT53_000701"
     CDS             complement(44044..44274)
                     /locus_tag="IXT53_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ4904552.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            44561..44899
                     /locus_tag="IXT53_000702"
     CDS             44561..44899
                     /locus_tag="IXT53_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4904553.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(45104..45343)
                     /locus_tag="IXT53_000703"
     CDS             complement(45104..45343)
                     /locus_tag="IXT53_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HAQ4904554.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(45319..45666)
                     /locus_tag="IXT53_000704"
     CDS             complement(45319..45666)
                     /locus_tag="IXT53_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF159 family protein"
                     /protein_id="HAQ4904555.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
BASE COUNT        13571 a         9791 c         8009 g        14618 t
ORIGIN      
        1 ccagtcttca aaaaaccacc aaggctcatt gtccccgaac atggtaatta cttgatacat
       61 actcgtgctc cttctttcag cttttctaga ctcatcttag tcacttttca agaaagttgc
      121 aagaattttc tcttataaaa gggtcattta atgaacatct taaaattgtt acaaatgaat
      181 ctgattgttt aaggcaaagt agcgataaag ttattgatca tccaaaaata gctgtcttct
      241 ggatcgattg ttggttcttt tgtaaagtat cctaaaaaac gtaaggaaag ataaccgtgg
      301 atcatactac gaagttccct gcttcgatga actttttctt ctttcgttaa ttgaaaagcc
      361 tcaaatatct gcaaaatgat ctgattcgtt tcatggattc catctaataa aacagcttca
      421 ttggtatgcg gtacactgat cagtaactca tacactgctt gatttgaaaa agcaaaggat
      481 cgataagttt ctgcatagat ctgcagtgct tcctgaccac tttttcccac aagagcccgt
      541 cgcagctggt cattaagttc ctgcagaagt ttcgctgtta gcgctgtttt gacatcacga
      601 atatttttga aatggttata cagcgaggga gccttgatat ccaagcgttt ggctaaatgc
      661 tgaaaggtta tttgctgaac atccatctct tctgctagtt ctcgaaagca gtcgatgatt
      721 ttctcttgag acagatttct ttttttcatt tccatgtttc ctctcataaa ataaatcctt
      781 cactctttca gtttaccacg aaaaagaaag agaaaaaaga gactttcatt gataaactac
      841 atattgtagt ttataataaa gagctgtaga tgaaaatttt cagaaagaag gagtctaatt
      901 ggctgcaaga ataaaggaaa aattatttag gaaaagagaa acttgggaac aaaacttgtt
      961 tgtcttatgg tttggaacat ttatggctgg tatcggattt agtttagtca tgccttttat
     1021 gtcactttac atagatacgt taggtaatta tagtacgtca caattgaact tttggagcgg
     1081 attgactttt tcatctactt tcttggtgac tacgcttatc tctccttggt ggggaaaatt
     1141 agccgaccaa aaaggaagaa aattgatgct gttgcgtgct tcattaggaa tggcaatcgt
     1201 catttctttg atgggttgcg tgactagtgt gtatcaatta gtcgcattgc gtctgcttca
     1261 agggattttt tctggttata tcagtaatgc cactgccctt gtcgcaactg ggacgccaaa
     1321 agaaaaaagt ggtcaagttc ttggaacctt agcaactggt tctgttacag gaacattact
     1381 tggaccgctt cttggcggga tctctgcttc agcgtttggc tatcgcccta cctttttcat
     1441 tacagggatc atcttatttt tcgtcttctt gttgagtctg tttttcgtcc atgaaaaatt
     1501 tgtcccagtg aaaaaagaaa acatggtttc tgccaaacag attttcaaag acttgaaata
     1561 tccacacgtc gtcatcggta tgttcgtcac aacgatgatc atccaagcat ccaacaattc
     1621 cattagtcca atcatcagtc tgtacattcg ccaattgctg catggacatg ggaatgttac
     1681 gttgatcagc ggaatcattg cttcgatacc tgggattgcc actttgatag ccgctccaag
     1741 atttggtcgc ttaggtgaca agattgggag tgaaagaata cttgcgattg gtttgggttt
     1801 tgcgattctg gtttatatcc caatggcatt cgtcacgaat gtttggcaat tagctgcctt
     1861 acgttttttg attggtatct ctgatgcttg tctgctccct gctgtccaag cattgatcac
     1921 aaaatattcg cctcatcaag ctgctggacg gatcttcagt tataaccaat ctttccaagc
     1981 aactggtaac gtagtagggc ctatgatcgg ctccagtgtt tccagtgcat ttggttatcg
     2041 tggcgtcttt ctttcgactt ctcttcttgt tttggtcaac ttcttgcttg tacgacacaa
     2101 tacacaagag atccatcagc aagaaactgc agaagaaaaa gcttcaggag tctccatagc
     2161 ttcagggcac tctccttctc atagacaata agcgtaaatg aaaaacactg cttgaacagt
     2221 tgtcacagac tgtccttgca gtgtttttgt ttaggcctgt tttttcaaga actcagataa
     2281 gaggaataat cccttttcca aggtttcttt tttacaacag taacctagtc gggcatgctt
     2341 cggtaaatca aaggcacttc ctggtactaa tagtactcct gtttcttcta ataaattgat
     2401 acaaaatgtc tggtcatctt cttcgatggc taatttaatg aaagaagtag agacgtaatt
     2461 gggagcgaca agttcaactt tcggttcatt ggctacccat tgttttaaaa tcacaaggtt
     2521 ctctgtaatg atctttttgt ttcgcgccaa tattttttct ttattcttca atgcatgtac
     2581 agcaagatca tctgacagga ctccgccgca aatcatcgtg tagtctcgat actttcgaaa
     2641 aacttctgcc aactcaggtc cagtagctgt ccagccaata cgaatacctg gaatggagta
     2701 ggttttggat agggaattgg ttgctatacc tttttcatag acatctacga tcgacaagaa
     2761 ttcaccttta tccgttaaag gcgcatatac ttcatctatc aatacatatg catctactgt
     2821 acgagcaatt tctgcgattt tttctaaagt tttccgatct agcaatgtgc ctgtaggatt
     2881 gtttgcacta ttcaaacaga tgatttttgt ttcaggtttg actaaggctt ctagctgttc
     2941 taaatcaaac tgccaatctt cttcttcctt caaatgaaca aagtcaacag cggcacctaa
     3001 agatttgggg atgtcatata attgctgata agtaggaagc atcgaaacaa catggtctcc
     3061 tgcttccact aacgcgtata gagctaaaag attagcacca gtcgctccat tcgtttgtaa
     3121 gatgttttcc ggatcgacgg tttgataaag agaagcgact tcttgtttga aaacatctga
     3181 tccttcgatc cagccatagt ccaatggttc gtttttgcgt ttttcgaaaa atttacttac
     3241 gctagtccca tctaagccaa cgagttcgtc taatgtcaat gcttcgatcg agctttgtgc
     3301 aatatcatat accgctttct tttcccattt atttagccat tcttctacac cgaaatttgc
     3361 gattttcatg tttctttccc ccttatatct tgatccagcc tacagcaagc aacccgattg
     3421 aaagcagtgc ggccacacaa atcagcccaa tcattacttt ttcttttccc gtaatttgat
     3481 gtttggtttc acgacaagcg atccagtaga agcaaaaacc aggaagatag gcaatactac
     3541 ataaaaggac ataagaaaat cctgctaaaa tgatgcccat caattcaaaa actacagcaa
     3601 tcccaccgat cactacttgt tttttgattc cttcttttaa agaaaactgg acttgataga
     3661 tcccgacaag tagataacaa atcaaaattg ccgctgtaca taaagaatag gcaaattgat
     3721 aggcataatc agtaactaag aaagtcaata agaatagatt agtcaagatt gcagtaacaa
     3781 ctaacgaata agttggggca ttttttttat tcgtttttcc ccaacctttc ggcaacagac
     3841 cgtctcgagc catcaacatc gttgtttctg ctgggagcat cgtccaagac aaccaagaac
     3901 caatgatcga aataatcaag ccgatattga tcagcgctgc tccccaattt cccacgattc
     3961 cttttaaaat atttgccata gcaggttgtg aaatagttgc taactcttct tgagataaga
     4021 cgccgtatgg caagatcgaa gcaaggatat agataaacaa taaacaaagc agaccaatga
     4081 tcgatgcttg ctgggcttct gaacgtttct tcgcgcggtt ggctaaaaca cttgcgcctt
     4141 cgatccctac aaagacccac atcataacca tcaaacaacc tttaatctgc aaccagatcg
     4201 ttccttgctg ccctgttcca ttcactagat tatcagatac gttaccccaa aaatcagcag
     4261 tgaatatatt cactttaaat gcgataaata caaatattaa aaataggaat aatggcacta
     4321 atttacagat cgtcacgatt gtattgatga cgctcgctcc ttcaactcca ttgtttacta
     4381 ataaagtcag accccaaata aaaatacttg caaataaaat agaaggaaca ttttgtccac
     4441 ctttgaatat cgggaaaaat tctcccatgg cgctcatcag catcgttgca aaagcaacat
     4501 ttcccagcca cgcagacaac cagtaacccc aaccactgat gaagccacct aatttaccaa
     4561 atgaagcttc cgcgtaaccg aaaataccac catctagatc tggacgcttt tctcctaaat
     4621 gattcagcga taataccaaa gctaaaatac caatcccaac gattatccat gaaaggatcg
     4681 ctggccctgg agctgcagaa ctagccagat cacttgtgat cccaaacaca ccgcttccga
     4741 ttgacgaact gacaaccaga gcaaccagtc cccatttgct aatacccgtc gatttctttt
     4801 ccatttccat caccctcact tataaaattg aacaaatcac ttgcaaaaga ttcccttcta
     4861 ttttgcaact gctcttgctt aacaaaaagc aactttattc atattattgg atattatgaa
     4921 ctttttacac aagtatttca agtataaaat gaataaaaat taattttatt gcaaaaaaac
     4981 agctatgcta tccaaaagaa aaggtgtttc ttttgttagc catagctgtt ttgattcatt
     5041 taatatcgct taaacgtatt gtaaaacttt tgtttcttga tatgcgtggt cgatcaaacc
     5101 accacccaga cattccattc catcataaaa tacaactgct tgtccaggag tgattgcacg
     5161 tacaggttcg tcgaatacga cttctgcacg attatcatcc aataagcgaa ctgtcacagg
     5221 aacgtcttgc tgacgataac ggaatttcgc tgtacacttg aattcttttg gcattggttc
     5281 attcgtcgta aagtggattt cacttgcatc caagcttgtg gcatacaatg caggatgatg
     5341 gaacccttgc cccacatata atgtgtttgt cgctagatct tttcctacaa caaaccaggg
     5401 ttcttgcgaa tctcctccgc caccgatccc tagtccttga cgttgaccaa tcgtataata
     5461 catcaagcca gcatgttgtc ctttgacttc accatcaaga gtgaccatat ttcctttttt
     5521 tgctggcaga tagttgctca aaaattgttt gaagttcttt tcaccaataa agcagattcc
     5581 cgttgaatct tttttcttag cagtcgcaag gcctgcgcgt tcagcaattg cccgtacttc
     5641 tgatttttcc atgccgccta gagggaacat cgtttttgaa agctgttctt gtgataattg
     5701 gctaaggaag taagtttgat ctttattatt gtctactcca cgaagcatgt ggaccacgcc
     5761 gttttcatcg cgagtgactt gcgcataatg tccagtagct acataatcgg cgccaagatc
     5821 cattgcataa tccaagaatg ctttgaattt gatttctttg ttgcacatca cgtctgggtt
     5881 aggtgtacgt cctgcgcggt attcggctag gaaatattca aatacacgat cccaatattc
     5941 tttttcaaaa ttcacagagt aataaggaat cccgatttga gcagctactt tcgctacatc
     6001 cttataatct tccgtggccg tgcatacacc gttttcgtcc gtgtcatccc aatttttcat
     6061 gaatattccg atcacgtcgt atccttgttc ctttaacaaa agtgctgtta cagatgagtc
     6121 gacaccgccg ctcatcccta cgacaacgcg tgttttgctg ttgtctgtca tgatatcacc
     6181 atcatttcaa ttagattctg aaacatctac gagttgaagg tcgcaacttt tcagtatttt
     6241 acattatacg tcatatttct gaaaatttca tcccttaatt atcaagtcgt aaaaatactg
     6301 ttctactgga cctattcagt acacaaacat gatagtcaac caaaaaatca ctcatctttt
     6361 gagcattcga tataaaaaat tggcatactc ataaaaaggg ccagcctttc ggcaataagg
     6421 aataatttta taaattgtga aaaactattt ttcaaaattg cttcttatcg cttaaagctg
     6481 cacgtctcat ttacaacctt ttctttttag atgttatgag ttgctcagat aagtgatttt
     6541 gtttactcta agaaaaaagg gaggcaaaag atacatgaat tactttatca gtgatatgca
     6601 ctttttccat gaacgcttat taggaaaaaa cgactttgct ccgcggctct ttgcaaatgt
     6661 ggatgaaatg aaccaacagt tgatcactag ctggaatgca gtaataaaag aaaccgacca
     6721 tgtttatcat ctaggcgacc ttgctatgca tccgcaatat gaaaaagcaa atgcagaaat
     6781 tttggaactg gtcactcaac tgcaaggaac aatccacttg atcaaaggga accatgacag
     6841 tcgcgctttt ttccgttatt tagaaaaaaa tgatcctagg cttactgacg gaagtcccaa
     6901 attttatttt tatgatgtag gtgccatcgt taaattcgac catcaacaat attacttgac
     6961 tcattatccg atgttgctcg ggcctaacga cactatccgc aatcttcatg gacatatcca
     7021 tcattacagc gtacctatcg agaacgatgt caatgtaggc gtagacgcac cggaaagaga
     7081 actcttaaag gaaaaactac catttggcac tccgttatct gagcaacaaa tcaaagaaat
     7141 ctatgaagca aagaggaagt acgataggtc gtgaaaaaac tgccctatat caagtattaa
     7201 acaaataaag ataattactc atatttttca tttgagtagg cttattgcca caggaacatt
     7261 aggatcagaa atggagttgt aacaagactt ctgtcacaac tccattccat actattcgtc
     7321 tttttgctca tggtgttctt ttaccagttt tttctgtaat tctttatcat aatttcgggc
     7381 agctgacgtt aaaaataaaa cgattccgca taataaaatc ccgattccgg aaaacaagaa
     7441 cattttttct acccctaaac gatcagctaa tggtcctgca aataataatc caattggacc
     7501 tgccaggttc atcaaagagt tgaagttccc taagacacga cctaaaacat tcgattcata
     7561 actctgctgg atcattgcca tcagcagtgt attgaaatat ggcgttgcac aacctgctcc
     7621 tgcattcaag ataagaaagt acaagaaccc ttgactatcg tttggaacca atccgcttgc
     7681 accgatcgtc aggcccataa tgatgtaggc tataataata ggtttcattc ggtctttcca
     7741 tgttcctata aaactgatca gtgcaccgcc taagagcatt ccgactgcgt agactacttc
     7801 cactgctccc gcctgtccaa cggaaccacc aaagtaatct agcgtcatca atggatacag
     7861 actgattgct ggcatgaaca acagcatcac aaatgcacca ttgatcgtta tgtaccataa
     7921 ccctttatta tccatcaatt gctgcagccc aaattttgca tctttaaaaa tagtaagcgg
     7981 aacttccaat atttcaggag aaacttttgg gatcttcaca aaaatcaata agccgacacc
     8041 aaaaactgcc cccaatacat ctaataaaat aaggtaattg acaggaacga ctgaaaacaa
     8101 agccgcacct agagctggcg caatgatgaa attcgctgat tggaccattc ctaactgtcc
     8161 attcgtcctt gttatgtgag aagatggaac gattgtcggc ataatcgact ggatcgttgg
     8221 catttggaat gtttgtgcga ccgaacgcat aaacaaagac acaaaaacta gccaaatcgg
     8281 gaaggattct gaaatcgttc cgactactgc taaaataagc gcgaaaatcg ctacaataat
     8341 atccgtgaca atcaaaagag cttttttatc ccagcgatcc actagcggtc ctacaaatgg
     8401 gcttagtaaa accatgggaa tcattcctag taaagtagca aaactcaaaa ttgtcgccga
     8461 accggtttct cttgtcaaat accagatgat cgcatattgg acgaccatac tagtgattcc
     8521 tgacaaaaac tgtcccgata aaaagaacaa ataatttctc ttccaattcg ttaaaacgac
     8581 cgactgttca ttttccataa acatccctct tttctttttc attttgtata gcgtttgcac
     8641 aacaatcatt atacgggatt cttttttcaa aaaatacctt tttccctaaa taacaatcgt
     8701 ttcaatttaa atttctttac ttagaaacaa taaatcagca aaatattaaa aataacaaca
     8761 tgtgttaaat ttaaaaataa aaaagacaac taatttgcgt cgtccttaca aagttagtca
     8821 tctaacaata ggccgtttca aaactagttg tctttttata tccacagatc attcgacttt
     8881 ttcgaataac ccacggctaa tcatcccatc catcagaaaa taaaatttat cttggctcat
     8941 tttctgtttt ggatctttga aaatattgac tgctttcaat ccatttgctg tagtgatcga
     9001 cattttcaat gtttgaatat cttttgatac agtgattttt aatttgaatc cttctttgct
     9061 ttgcggcgtg gcatctaatg gtcggatcaa ttgataattg cctccgtttg tttctgtaaa
     9121 tccattatcg cgcaatgtat atctttttgc atcaggatgc aaacgatagc taattggtgc
     9181 gcccagtacc gttgctgttg tttcaaatgc catgattttc acctcgataa aattagtttc
     9241 tctttattat aaaagtttat cctgattttt actaggtttt tcagttaagt tccctaaaaa
     9301 atgaaggcgt tctctcagac atctgttcat ataagaatga cttcactagt tattttttga
     9361 cagcacgaac taattgatca aggaaatact ggatgtcgtc ttcggtattt cccaaaccaa
     9421 agctgatacg gatggattct tttagtatcg gtgtatcttc tccatacatt gcttctaata
     9481 cgtgtgaagg ctcgatattt cctgctgtac aagctgaacc agtagaaata gcgaccccat
     9541 taagatcaag cttcatcagt agcaaatcat ttgcttcgcc aattaagcga agattcaaca
     9601 catgagggag tttattcgtt aaatcaccat ttacttgata agggatttcg tttgcttcta
     9661 atgtatcaag aatcaattgc tggaatcctt tatatttgtt gttacgtgct tcacgttctt
     9721 caggtgacag cagttcaact gcttttgcca taccccagat ccctgctaaa ttttctgtac
     9781 ctgcacgccg cttttcttcc tgctccccgc ctaaaagcaa tgcagggata tttactccgt
     9841 ctcgtttata taagaaaccg acgccttttg gcccattgat tttatgagca gagatactta
     9901 ataaatcgat tcctaattct tctggatgga tcgtttggtt tccgtacgct tgtaccgcat
     9961 ctgtatggaa ataagctgga tgatccgcta aaagttcacc gatttctttg attggcatca
    10021 aatttccgat ttcattgttc ccgtacatca cagagaccaa gatcgtatct tctcgtaacg
    10081 cttttttgac ctcttcgatc gacagattcc ctttttcatc tacaggcaga tatgtaacgt
    10141 caaacccctt tgtctctaaa tatttcatgg tattaagaac agcaggatgt tcgatagctg
    10201 tagtgattag atgtctgcct tctttttgac gcgagaacgc tgtttctaag atagctgtat
    10261 tatccccttc tgttccccca ctgttaaaga tgatctcgtg aggttttgct tgcagactgt
    10321 ctgcaatgat ttgtcttgct gtttccaatt tttcatgcgc gtggcgacca aatccatgta
    10381 tgcttgaagg atttccaaac gtgtcttgca tgattgccgt catcgcttca attacttgtg
    10441 gatgcatggg ggtagttgcc gcatgatcca gataaatagt tttcacttca ttcatccctt
    10501 ccaaattatt gctaggcaca ttctagcata aaatagcgaa caaaccaaag aagagctcat
    10561 ttttgtctag aggacgtttc atgggcatag aaaaaaggag ctgtgacaaa agtcttgtcg
    10621 cagctccttg aagctgctca cttctgtcac aacttctttc cctctttttt ttgtttattc
    10681 gaattctttt tcttccagac ggaataacgg actcatcggt gtattttcat ggatgcgttt
    10741 gaccgcttca cccattaatt ctgaacatgt aacgattgtt aagttttctg gatgacgatt
    10801 ttcttctgtg aaaacagaat cagtgataca aatatctttg attggtgcat tgtctaaaat
    10861 acttttcgca tgatcggaca ataatccatg tgacgcacag gcatatactt cttttgcgcc
    10921 gttttctttt aatacatttg ctgctcgtgc aagcgttgct cctgtgttca agatatcatc
    10981 taccatgata catgtctttc cttgaacatc accgatcaca taaccaccct cagttgaagc
    11041 atcatctgca tgatctacga tggctaatgt gctgttcaaa tattctgaaa gactacgtgc
    11101 acgctgaaca ccgctgtttt ttggcgaaac aactacgatg tcatctcccg tcagacctaa
    11161 ttgacggtaa taatgagcaa acaacggcat cgtaaataaa ttatctacag gaatatcaaa
    11221 gaatccttgt acttgaaccg tgtgcagatc taaagtcagt actcgagttg cccctgcttc
    11281 ttcgatcaag ttagcgatca atttcgcagt gatcggttca tgaggtttcg ccgtgcggtc
    11341 ttgcctagca tacccatagt aaggcaatac gacattgatc gttttcgcac ttgctcgctt
    11401 cattgcatcg atcatgatca gtagttccat atagtaatca ttgacaggtg cattcgttga
    11461 ttgaacgata tacacatgat cccctcgaat actttcttcg atattgatgg aaatctcgcc
    11521 atcactaaat tgcttcacga cgcttttacc taattctgtt ccgaacactt tcgcgatttt
    11581 ttcagctaat ggacggttgc cattcaaact aaaaatcttc aacgtgtcat cctgatactt
    11641 attactcatg tagtcctccc caaaacttct ctattcttcg atagtttaac atatttttcc
    11701 aagattttct gttaatttca gcaaattctt aaggaagtga aactgttccg ttactccaac
    11761 tggcaggaat gactttatga taatcgcccc agttgtcgtg tactttgtaa taacgttctc
    11821 ctgtttcagt ttcaaaataa gcgtacgctg tcacccaatg attgccatat gtactgccaa
    11881 gtactttcaa aataccaatc atcacaggtt tgccttcacg tatacgtttt gtagctcgct
    11941 gccatgaacc tagaagagtt gctcgtgcac gctcatgatt gccattcttc tttaaaaaac
    12001 tagaaatccc tgtactaacc tgaaaaggaa tcgtaggaag tcctagtggt tggatctggc
    12061 tacgcaatgc cttcgtcaaa accaacgaat cagccgtatt tttcttgcga atctcatttg
    12121 gaagcatcca ccccttacga tagtcttgat aataggcaag gagaactgct gccgcgtaag
    12181 taccacaaag atagcctgag ggggtagccc atttcttata cctcaaaaaa cgttcttctg
    12241 gcaatcccac ccagcaattt gcgagttcct ttcctgacct tttttcttcg atcaaaacgg
    12301 gaatatcttc ttttctgttg atccatctag ataactcgat tggctcttca ttgatcagct
    12361 tccatttttt acaagaaaga ccctcttgat aagtatattt tgctggagaa tattctaccg
    12421 gatgtcctga ttgatcgata atcacataac tttgatcttc aaaaaccaga gctaaaaaaa
    12481 gcaatttact ttttcttgac tgcagctgct gagtgctggc taatttttta atttttttat
    12541 ctactttgaa atataaaggt ttatccacta cttgattcag ttgggatcgc ctcctttttt
    12601 tcattataag tgaaggattg aaaaaaacaa atcagtttga ttgttcgaat tttcaatttt
    12661 ttatgttcta tttgttaatt ccttcatttt gtaagataat gggcacacga aacaaaaaag
    12721 gagcgatcga tgaatgaaca aagaacgatt gaaacaagat ttctatgaag cagtcaatga
    12781 agaatggcaa aaaacagcaa aaatccctgc agataaacca gctacaggcg gtttccaaga
    12841 tttagttgac gggatcgacc aacttttgat gggagaaaca gacgatctgc ttgcccatcc
    12901 agaaaaagtg tcttcagaaa aaatgaaaca tttccttgca tattatcaac tggcaaacga
    12961 ttatgaccga agaaatgagt tgcatgctca accgcttcaa cctataatcg aaaaagttga
    13021 atcattgcag tctttcgacg aattgaataa gcaatttcca gaatggctgt tggatacatt
    13081 accattgcca tttaatctgg atgtagatgc agatatgaag aatgcgcaaa cgaacgcctt
    13141 ctttgcttat ccgccttctt tattccttcc agataagacg tattatgcat cagaccatcc
    13201 caacggtgca caactaatag gcgttttctt tgatatggct gttcagttat tagagcttgc
    13261 cggcaaagaa aaaagtcagg cagaagcaat cacggaacaa gcagtgcaat ttgataaatt
    13321 gattgctcct catgtaaaaa gtgcagaaga aaacgcagat tacagcaaaa tgtataatcc
    13381 gcaatctttt gatacattta ttaaatacac agataaactg gacttgaaaa aacttgtcac
    13441 gggtttgatt gggacaactc cagataaagt gatcgtaact gatccagtgt acttcgatca
    13501 tttgaatgac atcctgacag aagctcattt tccgttattc aaaagctgga tgatcattaa
    13561 aacagtacgt tctcttagtg gttatctatc agaagaattc cgccaagtca gcggaatcta
    13621 ttctcgcgta ttgtctggaa cagacgaagc aatgcctcag aaaaaagcag cgtattacct
    13681 tgcctctgga caattcgatc aagtagttgg cgattactac ggaaaaaaat attttggcga
    13741 agctgctaaa aaagacgtag aacaaatggt gaaaaaaatg atcgccgttt ataaaaaacg
    13801 tctagaagaa aatgactggc ttggggatgc aacaaaagaa aaagccgtca taaaattaaa
    13861 taacttaggt atccaagtag gctaccccga taagatccca gcattattcg accagttcaa
    13921 aacagtacct gcagatcagg gtggtacttt gctttcaaat actcttgttt tcagcaaact
    13981 cacattaaaa gatcgtttca gcaaatggaa caaaccagtg gatcgttcag aatgggaaat
    14041 gagtgctgac acggtcaatg cttattatca tccatttcgg aatgtgatcg tctttccggc
    14101 agcgatcctt caagcaccat tttacagtct agaacaatca agctctgcta attatggtgg
    14161 aatcggagct gttatcgctc atgagatttc ccatgcattt gataataacg gcgctttatt
    14221 tgacgaatat ggcaacttga acaactggtg gacagaagaa gatcttacac aatttcaaaa
    14281 caaagcacaa gcaatgatcg aagaattcga tggtctgcct tttgctgacg gaaaagtaaa
    14341 cggaaaatta accgtttcgg aaaatattgc tgatgcaggc ggtctaagct gtgcattgga
    14401 agcagcaaaa acggaatcag atctttctat cgaggaattc tttgtcaatt gggcaacgat
    14461 ttggcgtaca aaagcaaaaa aagaatacca acaactttta ctgcaaatcg atgtccatgc
    14521 ccctgctaaa ctccgagcaa acgtgcaacc aaaaaacctt gacgaattct atgaaacatt
    14581 tggtatcacg gaacaagacg ctatgtatct agcgccagaa aaaagagtgc gaatctggta
    14641 agaggttgtg ccagaagtgg tcagcttcaa gaaataagaa ggaatttggg aaaatcgctt
    14701 ctcaaatttt tgtgacattt cggcttattt ttcgaagaag ttacttctgg aataccatgt
    14761 attagaggtt gtgaaaaagc tgcttagttt caagtagtaa gaaaaatttt gtgaaaatgg
    14821 ctttccacat ttttgcataa atttgactta ctaccgagaa actggctttt gaacaccgtt
    14881 tatcgagagg ttgtgaaatc agcgtcctga cctgagtatt agagaaaaaa acggaaaaat
    14941 agttcctcat attttgccgt ttgttgagct aataccgagg gtcagctgat tgaacaccgt
    15001 ttagtggaga gattgtgaaa aagctgtcct gcatcaagta ataagaacag ccaatcaaaa
    15061 gcagcaagga gaacctcatc tataaaaaga tagaaggaag tgagacatct attggtctca
    15121 tttccttcta tttttacttt cctcagtcaa ttgttttaca tcataaaagg agaaattcca
    15181 cttatttttt tcgctgtttc atgacataaa caactacacc gattaacaaa attcctccag
    15241 ctaatgctag caacgggtta ttaaattctc ccgtttttgg gagtccttga gtgccatttt
    15301 tttcactttc ttttgctgtc ctatccggat gagaaggttt tccatttggt tttccatttg
    15361 gtgattcatt tcctggattt tctctttctg gtttgtcatt tcccgggggc acatcggcag
    15421 tttctcgcac agtgatcgct ttttgctggg taacagattc tgtatgagca gaccattttt
    15481 ccccatcaaa tatttccgaa cgataagtga cttctagcgt atattctcct tttgaaggtg
    15541 ctgaaaaaat agcggtatga ttagaaaatt gagcacttga ctgtgtttca acgatttgga
    15601 tcttttctgg gaaataacgc atatcttcaa agacaggcgt tccttgattt ggtgtgaaag
    15661 ttgacacaaa aactgtaaaa ggcttcagtt caatgatatt tccttctgca ttctccgcca
    15721 caccatttgc ttcttgtata ataatcggat tattttgttc atttggtttt gtctgatttg
    15781 gttcttgtac gattgcatac tctggatatt ccgaagacgg tgaggtttct ccgtcaaaaa
    15841 tagcacctac agataatgga agaccatcca ctctttcctt ttctgtttga cgattccccg
    15901 gtttaatctg catcagtcca tgaacatgga tacttccatt ttcatagcgc gtaacaggct
    15961 gctgaccttc tttcgaacat gtatttagag actcgatcca atcttttgat atggtatgtc
    16021 caagagtatt ttctgattgt ttctctcgtt cattccaata gtagttcaac gagtggtaga
    16081 tttgatcttc gttttgattc aatgggcgga tctgctctct catctcaaga ttctctgttc
    16141 ggaaagataa gataccagaa gcatggtagt ctgtgtttcc tgctgaagag gtataaagag
    16201 ccacattata tgaaccgtta ttaaaacttg tactgctatt gacgataata tctgttccgc
    16261 tattggaatc gattcctttc gcaagatttt cgaaagacaa actattgatc aattcatgag
    16321 ggcccgttaa agaagaaccg cccattttga atccattacc gttaccttct ccctcaattc
    16381 ctggtaccca gccgtttcga taagcaactg agtttttgat gattactttt ccgatcgaac
    16441 ctgtttcatt tttagcaaaa aggtcccaac cgtcatctgc attatgatac gcgatacaac
    16501 cgtcaaatac atttccttct cctattgtca gtttggccgc aaatccatcg gcatcttcaa
    16561 atcctggatc ggcattttca aaagatgtac agttcttgac taagttatgc gcgggccatg
    16621 cttcgaaagg atcatttgag ctaccgctga tttggattcc tgtatttcca ttccgataag
    16681 catgaatctc ttcgatcaaa ttatgatttc cagaaatctg taatcctttt tgcatagctg
    16741 cactatttgt aatgtcaaac cctcgtaaac cccagtagtg actgaacagt gtcatcccat
    16801 ttccagttcc tttaaaatca agtacaggac gcttgttttc tggatttttc tctgcaatca
    16861 actgaatcgg gttttctctt gtcccgttaa cgccttttgg aatcgtcaat cctctttcga
    16921 aggaataagt tccaggcgcc atgacgattg tctgcccagc atatgcgtat ctcaatgctt
    16981 gtgtcagaga taatggattt tgtctgcttc cgtttcctgc atctgaccct tctggtgtta
    17041 cataaactgt cgaatattta ggtttacggt agtccactgt atgaagaatc tcttttgttt
    17101 cataactagt catctctacg tattcttctg gcggatagtt tttatctggc gtaaaactga
    17161 aagtaaattc gttcgttcct tccatcagtt ttgtctgata atcaaattcc cgattcgcag
    17221 tgatagccaa gttttttgcg ttcttaagca cctctcctgt tgctttattt ttaattgtca
    17281 ataatccgtc agcatttgct ctaaaggaca tagtatgatc ttggtttcct gaatgattac
    17341 tagaggtcac agtgtaaatc ggatcaatca agcgagtagg tctttcttgc ccaggaggat
    17401 ctgtcgcagg atctgagtaa gcaacttcga ttccttctac cttgatcgtc atattccttg
    17461 ctgcagcaaa tcctgcataa ataaccgatt catctgaagc gtataatttt tcccagtcat
    17521 acatgatgtc agaagccatt tcttctcctg tttcaggatc gtaataatat gcctctaaac
    17581 cagtattcgt ttttttcatc tctacgtcca gtttagtaat cgagttcgtg gccggaaaca
    17641 gattcccatg accgtttccg aatatattat aagaacctgt ttctttttcc aagaaccctg
    17701 ctgaagtctc taaaggtgtt gtttccactg agacactccc aggagctggc gcagcttgcg
    17761 gatcttcaga tgatattcct gtgattgccc gtgtccccaa tccaagtcgc atcgtatatt
    17821 tgctgccgct cgtgttgacc acttcttgtc tatccgagtc ccagacatat tcgatccgac
    17881 tgcctagtat ggcaaatgaa tttgaataaa atgcctgccc aaacaaatga ttttttggta
    17941 ctgcgtctct taccattaaa gcaaatcctt cttgggcatt cgtataggtc caagattcta
    18001 tttcgatcgt cgcccgcatc ctaaagttga tgtctttggg gattgcttga taataatagg
    18061 tcattccatc tgatccgctt gactggaatt ttccgccgtt attcgtggaa gagagccgca
    18121 cgccgtcttc taatggttca atggcatacc tactagcact tgtgctttct ccgaaaacaa
    18181 cccctttcca ctcttcattt gcttcttcag attgcttttt tgtcttttca ttttctgatt
    18241 ctttcatcat aggatcattt ccttcttccg tatccatatc ttcactagtt cttggtattt
    18301 cctccattgt tgacatccct tcaatgttca cgtgtgcatt ttcttcgttc acagactgtt
    18361 gttcgttagc agcaacttca acgacagaaa ttaaaaccgg ggtactgagc ataaggagat
    18421 tcatcattag cgcactgtat tttatacgtt tttttaacaa gtgtattcct cccgtaacaa
    18481 attagaaagc gtttacttaa agtattacct aatttatttt tactgtcaat aaaaaacaaa
    18541 attataataa gtgtatttta taaaaagaaa aaatataact taagttgttg ataataaaga
    18601 gatttattaa cgtaaaaaga ataagttttt ttaaaataac gtataaaaaa gagcataaaa
    18661 caaagtcgtt tgttttatgc tcttctttta ttattttgtg ctttttattt tatcaaatca
    18721 ctttgttttt ttataaaatt tctgcgccta gtgtggactc aaaatgtctc aatgcccatt
    18781 catgtccaac gggatcgaaa cttgccacgg catctttgaa aatatggagt ttgtatccta
    18841 gattatacgc atcgactgct gtatgcaaca cacagatatc tgtacatacg ccggtcaaat
    18901 aaatatctgt gatctggcgt tctcgcaaac ggatgtctag atctgttcca ctaaaagcgg
    18961 aataatgccg tttgtctatc caataaacat tttcctcttg ctcatagtct tggtataaac
    19021 tttttaaaga gccgtacaat tcccgtccac ccgttcctat cacattatgc ggaggaaata
    19081 agcggttctc cggatgaaag gaatcttttg gatcatgcgc atcaatggca aaaacgacaa
    19141 aatccttttg atcaataaac ttctttgtat gagcagtcag ctttttttca attacttgtc
    19201 ctgctgtccc agtagtcagc tttccatcct ctgcaacaaa atcatatgtg tagtcaatcg
    19261 agatcaatgc gttcatctta gtcctcctta tacgagatgt ttgacaaatt cgatcaccat
    19321 ttctgctgat cgtttgcctg cgtcttcgat aaattcgtca aagctttgtg tcgccgagtg
    19381 atctgctgtg tcgctcattg cacgtacgat caaaaatggg atattgaact gacgagctgt
    19441 ttgtccaaca gcggctcctt ccatttcgca agctaatgct tcagggaaat tcgttaagat
    19501 ttctttgatt ttatctggcg agtcgacaaa tgtatcacct gtaacgatca atccttcttt
    19561 tgcgttcaaa ttcgttgctt ttgctgcttt aaccatttca ctgcgcaaat atgtgcttgc
    19621 ttcataatat agaggcattc ctggtaattg tcctgggttg tagccaaatc ctgttgcatc
    19681 tgcatcaaaa taagctactt tgtctgatat aacgatgtca cctacttgta agccttcacc
    19741 gattccgcca gctgaacctg tattgatcac catgttcacg ccgtattgtt ggatcaacag
    19801 acttgtggta atagatgcta agacttttcc tataccggaa cgtacgacga tcacttcgtg
    19861 gttacctaat gaacctgaaa taaataaagc gcctgcgcgt tcccatgata atggttcgct
    19921 taggttctct ctcaaaattt ttacttcttc ttccattgcg ccgataatac cgattttcat
    19981 ctttttaatt ccttctttct atataaatgc gattgctaaa aaaaccacac ataacattag
    20041 gataacaata ataagagatt tcatcaaaaa agagttccat ttggtcgtct tttcccgttc
    20101 gctgatcctt gttttcgtga ttggtttatt ctttttgctt tctttacgat aaaagctggc
    20161 aatctttttt tcttcttgct gataagctgt ttctgctttt cgctgttttt ttaatgactc
    20221 gcttgcttgt gcttgttgtc gcttgcgaag ttcgcttcgc gtaataagcg gtcctttggc
    20281 cataagccga tccctctact ttcctttggt caatagttcc cagtattgaa tcgcataaat
    20341 cgtctttgca tcacaaatca actgatcttt catcgcttgt tgcgcctctt caagagttag
    20401 atgatacaac tccagtactt cgtcttcatc ttgtgctaaa ggattctcga ccttctctac
    20461 cccttgtgca tgataaatat gaaggacttc atttgcaaaa cctggtgaaa gatacatcga
    20521 agtaagatgg ctcagcgact tcgcacgata gccagtttct tcttctaatt ctctagctgc
    20581 tgtcatttcg ggattttgtc cttctcctgg atcgattttt ccagctggaa tctccaaaat
    20641 cactttttct aaaggttttc gaaattgttt gacaagaagc agacgatctt gttcgtcaaa
    20701 agcaatgatc ccgacaccgc caggatgaaa taccagttct ctcttcgaag ttcctccatc
    20761 tggcaaacga acttcgtcta cagcaacatc aataattttt ccgctataga tttctttgcg
    20821 actgattgtt ttttcttcaa attgctcatt gttcagcata tcttctcctg ctttctttgt
    20881 aatcataact gctttttttt catttttcaa tttttctcaa aaactcctta ttagtttacc
    20941 atagataaac cttagatttt ctctaaatat agaaaatcct tctttagtcc gatactcttt
    21001 atagtagtcc gtatgctaaa ataagtatgt aatcgaataa cgaaaagaat tcaatactca
    21061 acgataaatg acatggaggg tttattatga taaaacgaaa ttggccgtgg cttctagcat
    21121 tggtcttgct atttctcttt gtccgaaaca atagtttttc ttttgttcta ggacttttat
    21181 tgataggtgt agttttagtt ttgtggggac tctttggttc gcgcaggaaa aagggaaaac
    21241 aagaagaaat tcctgcactg actgacgaac ttgaaactca ctatgaaaaa agcggtatga
    21301 cagcgaatga aatttcattt tttcgacaaa caatgaatca aacaaaaaat gagatcaatc
    21361 aattgcaaat gaatatgcag cagacagcca aacttaaagc aatcgatctc cgccatgatc
    21421 cagtcaaagc agcgaaagct ttattcaaag aattggtgaa agaaccaaac cgactgcatg
    21481 aagcaagtca gtttctttat acgcatctgc cgaatctagt tgatttgaca aataagtata
    21541 atgagatcaa tgaccatgag atcaaaaaca aacagaccta tgaaaaacta gaagaaagcg
    21601 cgcagatcat cgaccagctg tcagctttaa tcgcacaaga ttatcaaaaa ttcgttgctg
    21661 aggatctaga tgatatcgac gtagagatat ctgttgcgaa acagagctta aagcgtgata
    21721 ataaatatga accagaacaa aaggattaac tcttttgttc ataaaagaag actcggaaca
    21781 ttcagatgat gtctggctgt ttcgagtact ttttctaaag tagagacatt catgtaagaa
    21841 tcgagaacag attcaacaag gaggaatttt ttatggatca taaaccgact gaaacaaaag
    21901 atgttacacc agttaacgat acacttgaag atttattgaa caacccgttt tcaactccaa
    21961 cagatacgct gaccacttca caacaagcgg agattgatca gctccaagag caacagacag
    22021 ctgctcgttt aattgacaaa ctaccggcgg aacgccaaga acaagctaaa cagctagcag
    22081 caaaaatcga tgctaatgat gcacagtctg tcatcagcta cggatcggct gcccaagcaa
    22141 aattaagtga attctcacaa tcgatgttga atcatgtgca agcacaagat attggtcctg
    22201 taggagattc cttaactgaa ctgatgtatc gtctccaaga agccaatcct gacgaactgc
    22261 gtgccggaga agggaacatc ttccagcgtg tcttcgggaa ggtcaaacaa tcgatctatg
    22321 aagtgacagc aaaatatcaa aagattggtg cacaaattga caagatttca gtgaaattag
    22381 ataaagaaaa agacggtctg ctaaaagata atctgatgtt agaacagctt taccagaaaa
    22441 acaaagacta ctttgatgca ctgaatattt atatcgctgc tggagaattg aaaatggaag
    22501 aacttcaaac aacgatcatt ccagaagcga tgaaaagagc agaagaaact ggcgatcaga
    22561 tggatgttca gattgccaat gattacacgc aattcctaga tcgtctggat aaacgtacgc
    22621 atgatcttcg cttagctaga cagatcacga tccagcaagc accacaaatc cgtttgatcc
    22681 aaaacacaaa tcaggccttg gctgaaaaga tccaagcatc tatcgctaca gccattccgc
    22741 tgtggaaaaa ccaagtcgtg atcgcattga ccttgcttcg tcaaaaagat gcagtaactg
    22801 ctcaacgcca agtctctgaa acaacaaacg acttattgaa gaaaaattca gaaatgctga
    22861 aaatctcagc catcgaaaca gcgaaagaaa atgaacgtgg aatcgtggat atcgagaccc
    22921 ttcaaaccac tcaaaatgat ttgatcgaaa caatccaaga gacattgcgt atccaaaaag
    22981 aaggaaaaga aaaacgtcgt catgcagaag tagagcttgg acacatggaa gaagatctga
    23041 aacaaaaact gttggattta acccaataga tcacctttcg cttgttacta aaacaaacgc
    23101 ccaacaacag tttattacaa ctgttgatgg gcgtttttct tatctttctt ctaacttctc
    23161 cattggagtc tatttctcat tcatcttgct gtgtccatag ccatagccaa aataaatgat
    23221 cagtccaata accattacga taccgaacac gatccatgtc acagtttgca gacgaagcat
    23281 caatacaata caagtcaaga tggagacgat tggcaatagc ggaacgaatg gcactttgaa
    23341 ttctccagct tttggtttgc caaacatttt tctcaacttc agtaagccta gactaacaag
    23401 catcaaatag gcgagcgcca tgatattcgt tagttccgct aatacattca atgggaaaaa
    23461 tccggcaaag aatacagctg ccacaccagc ggcataagtc gcttttttcg gtgtacgatg
    23521 cttttgatcg atttctgtca taaacttagg aagtaagcca tccttgctga cagcaaacag
    23581 taaacgagca agtgaataca tcatcgagat cgttaccgtc agtagcgtta aaattgcccc
    23641 tacagcgatc accgcgccca ctgttccatg tcctacatac ctcattgcaa aagccactgg
    23701 gtcattgact cctagttcag tatacggaac gattcctgtc aagatcagcg tcacgatgat
    23761 gtaaaggaca gtagcaatca gtatcgaacc aatgattcct cttggcacat ctttttgcgg
    23821 gtttttgact tcctcggctg ccattgcaac ggcatcaaat cccaagaaag caaaaaacac
    23881 aagagctgca ccatctagca caccggaaaa accaaaaggc atgaatggct gccagttatc
    23941 aggtttaacg aaaaatattc ctacaatgat aaacaacgcg atgataccaa atttgacata
    24001 taccatggca ttattcaaac gtaatgcttt cttggcttca agcgacacga tgtaagtgac
    24061 gaatatcact acgagcaccg caatcaagtc aatgtatgta ccattttccg gattgtaccc
    24121 gccagttaat gcttgtggaa gttcggcccc taaacttttc aaaaaacctt gcatatagcc
    24181 agaccatccg gaagcaacag aggaaaccgc tagtaaaaat tccccgatca acaaccaccc
    24241 tgtcagccag gcagcaaatt caccaaatac aacatacaga taggcatatg gcccaccaat
    24301 cactggcact cgcgaagcaa attctgcgaa acttagtcca gacaaaatga caacgattgc
    24361 agctaagaca aatgaaagtg acagtgctgg accagcattt tgattggccg caacccctgt
    24421 caccacaaat attcccgtac cgatgatcgc accaattcca agcatgatca aatcagacgt
    24481 ttttagttct tttttcatct cactcgtatg attgagattg atctctttct tacgaaacaa
    24541 atccattgaa gttcctcccc attttaggtt ggaaaacagc gattaaactg ttttcatata
    24601 ccctaaaagg ataccaccta aaatgactaa aaggcaacca acaataacat aaatgagctc
    24661 ttttttcgtt tttctttcgc caaggatgaa aatcccacct aaagtagaga taatgatccc
    24721 catttgtgaa agtgagaagc caacagctaa accaagtgat ttgactgtta gaagcatgca
    24781 gatgtttcct aagccccata ataatccaca gatcatgttt ttccacacaa actgatcgac
    24841 tttaacttta cggaaagcaa agaagcttgc gcctaagatc attccgatac tttgaggtaa
    24901 gataattgac atcgcatcca aattcgccca gttgacgatg atggtatata caccataacc
    24961 aatcgtagag aagatcaatg ctctaaaacc cttaccaaaa tcaagtattt tattatctgt
    25021 atctattttt ccttcgtcat cttgtcgggc agtcaaatac gcaccgctta caagaagagc
    25081 taaagcaata ataccgtata cataatcttt cgtttgtgtc cattcatgga agaaaacggc
    25141 tcccgcaatc gtattcaaaa ttaattgaaa accagtagac aatggtagcc ctacagaaac
    25201 acccatgtac ttcattccat ggaactgtcc attttgtcca acactccaag ctaagccgga
    25261 taaaaatccg atcaagaaaa tccaaggggt gattacagga ctaacaacga aaaagacaac
    25321 taaagaaaac agaaatgcac cgatcgtcat tcctagtgtt tgttgattgg catcgccacc
    25381 aattttacca cttactaaac caatactacc ccatgccaac atagggacta atgctattaa
    25441 aatttccata accaatctcc ttttatttca catgataaga aacttatcag cgaaaacgtt
    25501 aacaacacaa gcactttttt tcgtttctaa cgattttcta acgtttttca ttataaaaat
    25561 ttaagattgg gaaaaataaa gtcattgttg tcccttttcc ttcagttgat tcagccgtca
    25621 aatggatacc taatttttcg gccagatttt ttgctaaata aagtcctaat cctgttgaat
    25681 gtgttttgct aagtcgacca tttctcccag taaatccctt atcaaagatc cgtttcaagt
    25741 cttccgctgg gataccgatc cctgaatctt taactgaaag gctgactcct tgcgcttcct
    25801 tttctatgct cacaatgatg tttccgtgat ccggcgtata tttgatggca ttactaagca
    25861 attggttgaa gataaaagcg atccatttag catcggtcaa aacagtatga tcctcaccaa
    25921 cgatctcgta gcgaatattt ttttggataa aataatttgc ctgactgcgt atcacaggct
    25981 gaatgattcc ttttagtcca tattcttgga tgaggtagtc ccgcgagaaa ctgtcgagtc
    26041 ttgcataata caaaacttgt tctacgtatt catcgatttt tgataattca ttttctagta
    26101 atgtatactt ttgatcgtca atatcgaact cgatcgaacg aactagcaat ttagaagcgg
    26161 aaagtggcac tttgatttca tggacccatg agtcaatata atctttttgg tcttgttgat
    26221 tactgattgc tgattgcatt acttcttggt gttcacggat cagctgattg atatattctt
    26281 ctaccagttt ttcttcttct gtcctcccac cactcaaata attttgcaaa ggggaagttt
    26341 tttgtatgtc ggctaatttt ccccaccatc ttctcttgct caagtaacca cccagcagat
    26401 aaaaaatcag aaagacgccc tcaatcaaag ctaaataagc aattgtgctc aaatcaactt
    26461 tgatgtttgg cgccagccac atcacaaaga tcgtcaatgc aataaaaaac agccatccca
    26521 agatcaatgt ccactgatct ttcaaatatt taaaaaaatt catcttaacc gctcatcctt
    26581 tcagggaacg atatatccct gtcctacttt tgtttcgata tatccttgga tacctgcctg
    26641 ttcgattttt cggcgtaacc gattgatgtt gactgtcaat gtattgtcat ctacaaatcg
    26701 ttcgtcatcc cacagtgctc gcagcaattt ttcccgactt aagatctttc cattgtgctt
    26761 catcaaaata aataatagac gatattcatt tttgctcaaa tcaatgatct ctccgttgat
    26821 ttccatactt ccgttgtcaa cattcaacgt aatgccattg tggctcatca tttcactaga
    26881 taattctgtg tagttgtaag aacggcgaag taatgcattg atttttgcaa tcagtacatc
    26941 gatttggaaa tgttttgtca caaaatcatc tgcccccatg ttcatagcca tgatcatatc
    27001 catattcgta ttcctgctgg agataaaaat gatcggtact ttcgatactt cacgtatttt
    27061 ttgacaccaa taatagccat ccaaaacggg aagattaata tctagtaata caagctgtgg
    27121 ttcttctctt tcaaagtctg agaacacttg attgaaatcg gtcgttccaa aggtttcaaa
    27181 ttgccatttc tcaagttcct ccatgatcaa ttgtcgaatg atctcttcgt cttcgactac
    27241 catgattttt gccataaaaa atccctccat ctttcttctt gctactacaa tatcaaagaa
    27301 gcccttgtga aacaagttgg cttacctgga aaatgtcatt ttagcaaacg ttaaaaaaaa
    27361 ctggaaatat gcgatttaat cgcgtttgtc tcttccaagc agcttaaatt tattatgtta
    27421 ttcttaataa ttggtctatt atctgttatt tttctttcgt tgtttttgtc ttttaaattt
    27481 tcgataacac caataaccga aaagcgttag cactagcaat atacctagaa ggagtataag
    27541 taatcgataa agatggtatt tttttgtctg ttctatcttt gaagagactt cttctgtaaa
    27601 gggaactcgt cttcctgtca ctagtaatcg gtgagtgttc acggtgtaag gtgtacaagt
    27661 gataagtgtg actagatcct gcttttctct aattgctaat gtatttgttt ttgttggaag
    27721 gacaacaatt ttctcaacga cttcataagc gagcatccga ccatacactt caagatagaa
    27781 tcggtcatct tgctccattt cttcaagatc cgtaaagagt tttttctcag caataccgct
    27841 atgggcggta atcacggaat gtgtactctc tcctccagtt ggaaaagagg tgccttgcag
    27901 gactgtcgct cctctatcta acaaaagatc attcgtttca tcaaataaag gcaagctaac
    27961 attgattttc ggaatataga tggctccgat catatgttgt tcgtaatatt cttttcctgg
    28021 attttggaca tttctaaccg cttgctcaaa aggatctttg acttgtccca ttcctgggat
    28081 tactgatgta tgagcaagcg tctgattttt cttttcatgc actgctaatt gttgctcatg
    28141 tttttcttct ttatcgcgtt caagctgctt ttgataattc tcgatccttc tttgatccaa
    28201 ataattgctg agagcatctg ctaaaaatgg atatgtaaaa actgcagctc cggcaagaaa
    28261 catcactgcc ataaataatt gtaataaaag actgatttgg gtttgaggtc tattacctat
    28321 tttctttttc attttcttca ccttcttatt taaaaaaact gcagcaacct ttataaaacc
    28381 gattgctgca gcttattttt gttgcttata cttcagactt cattttagat ttacggtacc
    28441 agctgtacgc tccaatcatc aaacttattc cgattagtaa gaatgccaag agcccgttcc
    28501 caccagtagc aggaagtgac ccgcgtttca tattcggtac tctttctggc cctggatttt
    28561 ctactcctac gatctgaatt tcttcattat agcttccata agagatgata aattcagtaa
    28621 aggctgcctc acctgttgga agtacataac cttctggcgc catggtctct ttcatttgat
    28681 aggttccata atctaaccct tgaacctcaa agtatccatt tccatctgcg acaaattctg
    28741 ttgcttgtcc gcttgttgcc cactcaattc tatctgctcc tgattcataa acagctaaag
    28801 gcgactcccc tttataaaat attgcgtatt cagaacggtc ttcattccac aattcgaatc
    28861 ttgcatcttt aagcaattca ctgcttgatg catcatattt ttcaaacttt cttccacctg
    28921 ttgtgacttt ctctggcggc tctactcctt cttcataatc aaattcatca tttcgtccga
    28981 tttcaataac cgctgtgttg ttatggaaat catccgcttc tagttctgtg gaaaccttca
    29041 aattatacgt gatcgttaaa tctcctccac gtgcatctgc taatctacct aaagtatcta
    29101 ctttggctac tccattttct tctgtcaaaa tcgtcacgac aaacccgttg cttactactt
    29161 ctactatata atcttgtcct tttgttaaaa tcgtctcgcc ggctcgcact tctatttcct
    29221 gatcataata agttagacca actgtaggcg tatctgtgat tttgtaattt ttgataacag
    29281 cgcttccgtt ttcaaggaca gaatcgatga aataaggaat aggtgctgtg atcgtgtaag
    29341 aagccgtttt tcctctttct agatcaatat aagaaatcgt ttcttgttct ccagcttcat
    29401 taataatcgt tacttgtcta aggtcgcttt cctctacatt catctgttta tctgcttctc
    29461 tgatttcgtt tttcggatac aaatgcacat ctgtattgta tgtgtctttc catgtagaac
    29521 catcccacac gccatccacc atatcaggca tcatcactgg catcataaca accattggag
    29581 acgcgattct ggaaatatga gctggcgaat aagtttctaa gaaaagatag actttatctc
    29641 tagaagaagg tgcttcttcc gtcacttgga cggtagagaa taccgcttct ccagcttcat
    29701 tcgttgtttg tttgtcaata cttgtcagtc gatattttaa aaaccaactt tctgtgtgcc
    29761 gtttttgaat ccattcaatc gcttcggcag aattaagacc gtcttctcgt tccggtgtta
    29821 aaggattatc tcgaatcaaa tcgtaatata cttccgttac gtcaaacaaa tcaaaatcga
    29881 ctcctggaat attttcttca ccaaaatccg tagaaaccaa tccgctattc ggtcgttctt
    29941 ccggaacaga tgagaaacct tttttgtgta atgtaatcgt cacttcactt ggtttttgtg
    30001 cgacttcttc ggctttgact agttttccgt tcccgaaaat ccctattccc gcagtaacga
    30061 gtaacagtac actcattccc gtgacaatca tttttttatt catttttttc tccccgttct
    30121 ttttttatta ataaaacagc acctaacaat aaaagaattc ctagtaaaga aatcaacgtt
    30181 cttgcttcat tcgtttgcgg gaatacggac cattttcctg attgacctgg attgcttgga
    30241 ccactcggct ctttcggttt gtccgttgtt tcttttacgg tctcgttata aacacgtggt
    30301 gttgcggatt cataggcaga gactggaact ttcccggatt ctaattcagc catctcctgt
    30361 ccgttaacta ctacatattg tggttggcct tttgcatcca cccaaaattc agggatgatg
    30421 acctcgattc tcttacttgc ttcatccatc tcgtaacctt ccacaccttt taattcttca
    30481 aaataatagg tgccagaagg caagaaacgt tgtcctgtcg taacaaggcc ctctttatcc
    30541 gaaatgaatt tgcttacatt tttgtcattc aaaggatcat tgtctgctgg tttgatccat
    30601 tgatttttca agtcattagc aggagccata tctaaataat atttcccccc ttctatcatc
    30661 tgatatagag caaaaacagc accttctaat ggttttcctt tttcagttgt tcctctttga
    30721 cgtccatact tgaagaagta tggatctctt agataaccga catttttagg atagatatga
    30781 atctcttgca gttcctcggt tgattctgtc gggtgataaa ttggcaaaat agcagcaatt
    30841 gggatagctg tctgatcgat atcaacatcc aatccaactt ctggatcaat cgattcttcg
    30901 aaaatcaaat aaactgcatc ccgatcttgg tttttcttag gtgctttgat acgtgcaatc
    30961 ccgtctccta atccgccaac cgtatctttc ccagttgtta ccgaagtccc tatttggaca
    31021 agcctttctt ctgtgattag ctgacggata ttgtttctgt tcgtttcagc gatttgctgg
    31081 acgaattctt taggagtcat gttttcagca gctttttttt catgataata atcggtcatg
    31141 tcgtaaatgg caaatgtcgc tccatttagt ggcaccgtct gtgaaatgat atctgctgtt
    31201 tcccctgctt cctcagagat agccaagcca tcattttcat ggtattcaaa ttgatcgtta
    31261 taatcgatat ctcgaatcat ccgtttatgc aacacgaatt ccacgacatc ttcccctgat
    31321 tcttctgccg acatcgcatg tggaagaagg actgctgcaa gcggtagtaa aacaagtaat
    31381 tgaagcatca aacacaacag ctttttcatt accttgctcc ctccttttta gattgtctga
    31441 caaacaaacc ataggctgct cctgcaagcg ccatgaagaa tgcagcaagc aggatataga
    31501 ataatcgtcc ggatcctcct gtagcaggca acggattttc aggatcgttc ccgacactaa
    31561 gctgtaatct gattcgattt gtctccaaaa gttcatattc taactcttgc tgtagatcag
    31621 gcgtctctgc ttcttcgttt ggataatacg aaatctcaac ttttagtgta cccgcttctt
    31681 ttcctggaac tacccgatca ttttctatgt caggttcttc tcgataacta atttctaaaa
    31741 taaagtaacc tggaagtttt ctatagctgt caggtgactt cacttccttg atccgataga
    31801 ccccttccaa caagtctgaa gcattgaata gggaagaatc gtttggatct ggtgccaagc
    31861 ctccctcgat gatttcatac tctcctgcac tattttgttt ctcaattgaa aattctgcgc
    31921 cttcaagacg tcggctattc atactgtcaa ttttcaaaag ttccaaatcg attggcgcta
    31981 gttcatcttc taaggttact tgtagtacac catcgatgat ttcatagcct tccggcaagg
    32041 gagcagtttc gtcttcgatg accgtatctt caatgataaa cttaccttct tcattcagtg
    32101 caaactggaa cacttgatca gatagccgat acccatttgg tgcgacagat tccgagacct
    32161 catattcacc aatagacaga tgcagaggtt ggtcgtaatt taaatcgaca tacttttcta
    32221 ctcctacggc acgagaatag aagtatccga tcccaggcaa acgtttcgat tcttctggtt
    32281 ctaacggatt accagtgtat gctcgataca gcgggaagga agaatccccc actttcctga
    32341 tatcgaaacc tgcaccatct aatgtatggc cttcatggtc taattttcta actcggatct
    32401 cccgataatc aaacgtattg taagcagtga atgctaccca taaacgatcc tcttcatctg
    32461 ttgtatacgt gagatattgc caattttctt cacctgtttg cttgagttgg atagtcccta
    32521 tagcattatc gtaaagtcgc aagatccatg tgtcattttc atcaggttct ttgaaacctt
    32581 ctgggttctg gctctcaatg attttgtatt ctgcatagtt ggtagtttct tcactatcct
    32641 tataaacaaa tgaatctggc tctccgattg gatgaataac tttctcacca tccacgatta
    32701 catatttccc gtcttcatct actgaataga acgtgagtct ccctttctct tcctctactt
    32761 cgttcacacc agaaagccct ttcgctactg gatatccgat ttccagtccg ttaccatctg
    32821 tttcataaag ttcaaactct gcgccattga ttggcatatt actgccttca attagtttat
    32881 tgatattcat gtggattggc ttgaattcat ttcgcatttc cagtttgaca tacaactcat
    32941 catactctgt gccttctatg ttaggctctt gtacaacaga aagcttgtga tattctcctt
    33001 cataatagga attcggatct ttaccttccg gtccgccgac tctcgaacca gatgtatatt
    33061 catctacaag ctcgtagtga cctaacatct catcttcgtt ggctgcatat tttttgattt
    33121 ggaattgata cgtctcatct actaactgat aacgtggtgg tccagcgatc tctctgatcc
    33181 aaaatatgtc gctgactgct ccagtttcat ctcctaaaat cggcatcttc gtttcaaaga
    33241 tcgctattcc gtccgtggta gttttacttg tttcgattgg ttcggcttcc ccactctctg
    33301 gattttggtc atataatgca aattcgactc cagccaacgg ctcgccattt tgacccttct
    33361 tgctgacttg gaaattacgg cctttatgtt cgtaacgaat ggaagaagat gggaaataca
    33421 tccgtccagt agtgatatca ttctcataat cctccaggtt ctgaatatag gccatattgt
    33481 tcactgtgca aaaatttccg tcttgagctc ccgcatcaag ttttacttgg tacttgatcc
    33541 tgaccatttc cccttcacct aaagaaatcg gagaaaattt ataagcttgg ttttcccgtc
    33601 cgacatacgt tgtcggagtc gccactactt ctggtggggc ttgtccatga cgttcttcat
    33661 cccatatttc tggctcttgg tacttcatgc cgttttctcc tggatctaga tggtaaaact
    33721 gaatatctgc cgatctgcca gctccaaatg gtgagacatt aaaaccagtt tcatcataaa
    33781 gtgtggcttg ctggatcgtt ctcttgaagg tagaagagat atcctccaag gctgtaacaa
    33841 tgtcattctt gttgttcgca ttgtaatatc gcggtgttac gccatcttct tctaaatcag
    33901 caatattttt caatacattt cgtccaatag catctagtcg ttgtctcccg ctaacgtttc
    33961 catcgattcc taaaccaatg gagaagatac ttggcgggtt ctcttgatac ttgtttttca
    34021 gccaatgatg ataagcaact gtgttgatcg cagatgactt ttcttgcgaa gtaggactgc
    34081 cattcgtatt gttattagaa gaaccatcac caacccagtg agtcgcttta ttatctcctt
    34141 cttttacctc tccataacga tagtgtacat ttacttcatc ttctggacgg ttaaagtcag
    34201 atgcataggt aactggatga gtaaaccctg cattagaagt attcccagaa aactcctcaa
    34261 aatttctgaa caaaacgtca ttatcttcag caatcttatc ttccatcaca gtccagttgc
    34321 tccaagctcc attagtgctc gtacgataac ttgactgaat cggtgcataa ctgaaggtcg
    34381 gtgtcccatc tcccacaacg attaggattt tttctggatt tctattttct ccgccattgt
    34441 ctttatacaa tgtttcatag ccatttttaa gccctagtgt aagcggagtt cctccgattg
    34501 gtgtacgatt taatgctgta ttctcacttc ccgtcaaacg attgatatca tttgtcaatg
    34561 gaaagatgtc cgaactgatt tgctcatggt tattgatagc tgtactatta tggtaaaaat
    34621 tcaccatacc gattcgaata tccagactcg ggtcagagag cagatcttta ctcatttcgt
    34681 ttactgcctc aatcaacgca gcatttttag tttgactatt ggtcccagcg gttaattcac
    34741 tcatactcgc tgacttatct agaacaaaaa cgatatcgat tggactgatt tcctttttct
    34801 cgcccccgat gacatctaga tagacattgt aaagggttgt tgcatcttca ggatgtagat
    34861 cagatgctgt gctgcttggc agaacaggtt ctacccattt tttgatgtac gccccgttat
    34921 aatccaagta actgttttca aaattccgtt catttcccca aagctgaacg gtttgagctg
    34981 gaacgtttct atcttcttcg cctgcagttg atccattatt agtagcatag tcgtaatttc
    35041 gccagttcgc tcccccgttt gtataatcac tatcatcgat caagttctct ccgtcatatg
    35101 gcgagccttt gtctccttga tagcgttcat tgctttgtgt ccaatttcct aacacttttg
    35161 tatagtcatt cgttgtaaac ttcatctatt ctttcatcaa ccaccacttg aagaggtagt
    35221 tacccttcgt tcacatcggg gatcttttct gtttcaaaaa tcaatgtgcc ggtttctgct
    35281 tcttttgaat aaacttttcc gacataccat tcttgaccat cgatcgtctc gacttttagg
    35341 tcttctccat cttcatcata ggaggataga cctgttccac gaacgttttt tacggtacca
    35401 atcccggcgg ctgccgcatc gattgctaaa cgtattctac cttcattggc actttccttt
    35461 ttttcaaaag ccagatgcca ttcaagggtt tcttcttttt caatatactt cccttcaatt
    35521 tttgcatagt cctcatctaa gaatacctca tcctcctttg tttctgttga ctcagcagct
    35581 acatccaggg tcgtcatatg atagactgac tggaaatatg gtccaaatag caaactgaca
    35641 gttactaact gcaaaagctt caattttaac cctttcacat ccttatactc ctttcaaaaa
    35701 tatttgtctt ctttcacaac aatcaacaaa acaatgccca tctaattata atttaattta
    35761 attttaattt taaatcaaat gatatccttt ttagttaaaa aacaccaatt atacaacatt
    35821 tattattaaa attaaattat aaaattttat aacgttctca tttaacaaaa cttaagttat
    35881 taagacaaat gaataaacaa ctacgctttc ctcgaaacaa aaaaataaag cacaaatgcg
    35941 catttacgca ctcatgcttt attcgtacaa ttaattcaga taacggcagc gcataggatg
    36001 ccaataacac ttcctaaaat agctcctcct aaaatgtcag taggataatg taaaagtaaa
    36061 aaaatcctcg aataagcgat caaacaagca aataaaaagg ctaatattcc tgcaactgga
    36121 cttaattcga acaacgcagc tactgataaa aaggctagcg ctgtatgtcc tgaaggaaaa
    36181 gaagaagtct tcggtttact tgtgtaaagg acaagccgac gatttttttc ataaggtctt
    36241 ttccgtcttg tcgttttttt gataaaaagc aaaccaacaa aaaaatagat caaactgccg
    36301 ataagaatgg ctagaccata ttctctagta tcaggtatca agactaaaac agcagcaagg
    36361 agcaaccaaa taaaaccacc attagagata ttactgataa atttcaaaaa attgattttt
    36421 cgactatccg ttttccgttg cttggcgaac ttgttgataa tactatcatc gattttagca
    36481 atcaaacgca acatgttatt cctccttttt aagaaccttt tcttgcgtac gatggataaa
    36541 aagcaggact tgaaaataaa acaagaaact gaaacctaat aaagcaaaat agattggtcc
    36601 tgcaaaccag aatgcgacaa gaaggatcaa cgtgcctgcc aaatagaaaa gcgcgctttt
    36661 gaaatcatta aaaaacaaga aaaatgcagt aatccaagaa gcttcttttt gttccagttg
    36721 aagcaagatt ttgtaactaa agagaagcgc gcttaataga agcaaaaaca acgtagtata
    36781 catcaatgta tagccaattc ccccacccca atagcggaaa atctgataat cgatcaatag
    36841 aagaagaaaa taaacacttg ctaaactatc ttgctttcca taaagagaga agttctcacg
    36901 aaactttttc cagaatagtg aaccgagctt tttctcttca ttttttttgt aatgataaat
    36961 catatacaga actgttcgac ttgcagttcc taatgtgaaa acaggcaaga cgcacaggaa
    37021 ccatagtagc tgtaaatgga taagacgtgg aatccattca atgatttcta agaaataatc
    37081 attggctttt ttaccaatcg atcgattcat catccttttg aacctcctgt caaatccatc
    37141 ccttcaatga aataacgttg tgcaaaaaag aacagcaaaa tcgtcgggat caagacaagt
    37201 gttgctcctg ccatcagata attccattgt gtcgttgctt ggttctgaaa aacttgcaag
    37261 ccaatctgta acgtgtattt ttcttgatct tggatatata acagaggacc taagaaatca
    37321 ttccaagctg cgttgaagga attgatcgca atggtaatca aggcaggttt cgtcaatgga
    37381 agcatcaaat gactccaaat atacaaatga ttcgctccgt cgatttcagc agcttcgatc
    37441 aattcattat tgatcgataa gtaaaattga cgcatcagaa atatattgaa cgcattgccg
    37501 aagaacgatg gaacgatcag gggcaaataa gtacctaccc agccgatctt ggagaataat
    37561 acatattgag ggatcatcgt tacaaacccc gggatcatca tcgtagaaag aaccaaagca
    37621 aacatcagct ttttcccagg aaaatcaagc ttcgcaaatc cataagcaat aaaagaattt
    37681 gaaaggacat taccgatcac tactagcaca gtgatgaata atgtatttct tgcatagcgc
    37741 caaaaaggaa aggcggcaat cgtatccaga tagttttccc atacgatttt ttctgggaaa
    37801 aaggtcggag gataacgaac gatttcttcc attggtttca atgaagtaaa caccatccaa
    37861 agcaatggaa tgatcaaaat gattcctatt actgtcatga ggacataact tagtattttt
    37921 tttgtttgcc gattcacact ggtctttttg aaataatcca tcatttgccg tcacctccag
    37981 aataatagac ccacttaggt gcaagcttca aattgacaaa cgtcagaacc aacacgatca
    38041 gaaacaggat cattgacata gccatcgcat aacccatatc aaaagctacg aacgccttgt
    38101 tccacatatg cagattataa aaaagcaaag aatttgcagg tccgcctgtt ccatttttcg
    38161 tcatgacata agcttcttga aaaatttgga aagaaccgat cgtagaggtg atcacatcgt
    38221 aaaaaataat aggagtgatt aatggcaaag taatactttt gaactttccc caaacgcctg
    38281 ctccatcgat ttctgctgat tcatataaag atctaggaac attttgtaat gtagctaagt
    38341 aaagtagcat tgctcctcca gaactccata ttttcatcaa aatcaacgca ggtttcgtcc
    38401 attgtggatc aaacagccaa gctggtccag ggatatggaa ccagcccaga aatgtgttga
    38461 tcaatcctgc agatggagaa agcagctgca tccataagat gtagactgct actcctgata
    38521 agactgccgg taaatagaaa gctgtacgga atacactgat tcctttgatt ttttgattga
    38581 gtactgtggc taaaaacaca ctgaaaatcg tcgtcagcgg tacattgaag atgacgaaat
    38641 aaacggtgtt gtataacgat ttccaaaata aatcatcgtg aacgaacatt cttttgaaat
    38701 ttgctagacc aatgaaattc atcttggatg tcaaattata atcggtgaaa ctaccaataa
    38761 ctgaaaaaag cataggccct agcataaata ccaagaaccc gataataaaa ggcgagataa
    38821 ataaataggc ccatcttgct tcttttttct ttcggataga taaacgcttc aaattttctt
    38881 gcctcctctg tacctagaca gtcaaaaatg gattattgat tttgcttgac taagttttct
    38941 actgcttttt gtgcatctgc caatccttct tttggtgatt ttgttcctaa aagaatctgg
    39001 tcaatctgtt cattcactaa tgtcgtgtaa tccggagcat ctaaaggaac tggtgtgatc
    39061 accgtatttt taaagttttc gtccgccatt tgatagatca tttttccttt atccgttagg
    39121 ttgtcattat tcactaaatt ctcattggct gtcttgctcg ccatgatatc aaaacttttt
    39181 tcgccgaatt tttcttgtac ttctggagaa gaaagatatt tgatgaattc ataagaggcc
    39241 tcaggatctt ttgctccgta tggtacttcc aagacgaatc cgccacccca cgaccaatgg
    39301 cctgaacctt cttctttttc tgggagctgc atcacgccaa aatcaaaatc atccggtgca
    39361 ttttccatca aactagagta ataattgata ttttgggcac gcatggctac taatccggaa
    39421 ataaatggat cagatacacc agacccaaat tcggcttcta atttattgat cgtatttttc
    39481 ccataataat cttgccagtc ttggatccac tccaaggctt ccactttatt tttagtatca
    39541 atcttgactt tttcatcttt gtcaaaccaa ctagtcccgt tatctgcgtt tagcgcccat
    39601 acatcagccc ctaaattcca taatggataa aaaccgatgc gtgtaaattc ttttccttct
    39661 tttttatcta gtttacgtgc tgtttcttct aattcatccc aagttgtcgg tagatcttgt
    39721 tcactgattc cagcttcttt aaataacttt ttattataaa aaatgacttg tgtatctgta
    39781 ttgaatggga tcgcataagg atcaccttca tattctactg tatcccaaag ctgcggataa
    39841 aaatccttag aagtgtcttt ttctacatat ttggagagat tagttgcttg ctgtgcttct
    39901 gctctttggg caacggaatt tatgtcctgt acaataacat cgggtgcatt tccagctgtt
    39961 attgcagata aagatttagt ccagatgtca ccccatggct gatacttata atttacatgg
    40021 atctcgtttt gtgattgatt gaaatcctca atgatttctt cgatagcttc ttttcgtgca
    40081 ccggatcccc aaaaggacca gaaatcaata ttttttcttc catcacttgt tttttcagaa
    40141 gaatttgctg cacttgaact ggcatcgctt gcattacttg atccgcatcc tcctaataaa
    40201 aacaatgctg aacttgccag caaccctgta aacaataatt ttttcatatt ttacctccaa
    40261 attttaatgt aaccgatgcc ggttttaata taacgatatc attttgttct gaaaataaca
    40321 atttataagg tatgattcta aaaaaattat taatgatacc aatactttat ataattcgcg
    40381 agcgctttct ttgaattttt taatctttcg tagcagaatg agaatgtatt aaaaatagat
    40441 taggaggaat tttgatgttt agacaccaga aagaattgca gtttgaagca aaaccagatc
    40501 gtccgaatcc attaatcgcg aaatatctgc aggagctgat cggcggccaa tatggcgaga
    40561 tgagtgtagc tatgcagtac ctgttccaag gctggagctg ccgaggagaa gaaaaatata
    40621 aagatatgct gatggacatt gcaacagaag aaatcggcca cgtggaaatg ttggcgacaa
    40681 tggttgctcg tttattagag ggtgcgcctg ttgaagatca agaagcagcg atgcaaaaag
    40741 atccaacaat cggtgccatt atctctggaa tgaatccaca gcacgcgatc gtagctggct
    40801 taggacctcg tcctgctgac agcgtgggca atccatggag cggtgcctat atcattgcga
    40861 gtggcaactt gttagcagac tttagagcta atttaaatgc cgaatcacaa ggaagacttc
    40921 aagtagcacg tatttacgaa atgatcgatg atcgtggcgt aaaagatatg ctgtctgttc
    40981 tccttgcaag agatagctat catcaaaatc tatgggaatg tgcagtgaaa gaattagaag
    41041 aaaaagaagg ttcaatctta gtcccaagta ctttcccgcg tgacaaagaa cgaaaagaca
    41101 ttgcttatga cttctataac ttctcagagg gagaccaaag tagtgaaggt tcttgggcta
    41161 gtggcaaagc actggatggc gaaggcgaat atcaatggtt gaaagaacct aagatcgaag
    41221 gcgctgcacc aaaattagaa ccagtaacac caaaaatgta tgggacacct ccattcaaga
    41281 aataaatatc tacccgtaga aacacccgtt ttttgttcca cacgctttgt gagcaaaact
    41341 gactaaagtc attaatcata accacccttc aaaagggtgg ttttctctag agctataagc
    41401 tcaaatcacc ggccagcgcc taaagacgct ggctttcata ttttatgttc aagccatatt
    41461 gcctttgcca gttttgccca cctcttaaaa ggtttatgta ctactcctta gcccttaaaa
    41521 ggatcgtctt actctcgtac actcagctta tctaacgcac tatcatgttt ttcttgttct
    41581 tgaatgtact ttttgatggt cgcttcattt tgtcctactt ttgtcacata gtatccttcc
    41641 acccaaaagt gtcgataccc aaatttatat tttagatttg cacaatctct tgatgatctc
    41701 ctgaatattg gctcgatatt gattataaat tatttttctt ctatactttg gtgtgaatac
    41761 aatgtggtat ttacacatct actttgtacg ggctaaacta tttcccttta ttgctccacc
    41821 cgcatagcaa gtggttttgt atttcaatcg ctcggcgcgc tcaattaggt ctaaagacaa
    41881 taaatctaaa aacccgctgc ttcactgaca gaaacaacgg gttttcagaa aaaaatgaaa
    41941 tgaaaaagat ctcttacggc aatctatttt atttttttga ttttttataa atggaaaatg
    42001 cttcaagaag tccaagaaat aaagccgtat aaaaacaaga acctatgaaa tctttattta
    42061 ttagatattc aacagaaatc ccgataacac tagccaacaa tatatttatc agaagtgtaa
    42121 aataaggatg ctttttatag aatagaacta gtgttgtttt catagactct cctttcttca
    42181 tatttccaat taatcacaaa ttcacggcat tactagaaaa attaaagggg cgcagaagtg
    42241 gaaaaatact cttcgacttt ccctacaaac caggccatct tgtgtttaat aactatacca
    42301 aatctaaggt acctctaaat ctaaattcca tttgtggcgg tgacctccaa tttttaactc
    42361 ttctatctcc catccagtat acataacctt gatacttcga ccacttatat atcgaacaca
    42421 caaataccta tccattgcag gagtactacc taacttagtt gctacttgca tctaaataac
    42481 agctgaaaag ataggactta ctcttagtaa agtatctgaa ctattaattt ttaaactatc
    42541 atttttatcc tcaactacat gcacatctgt tgcaacttca ctttctgtct catccagata
    42601 aatgattaag gaatcgggaa ttgcaatatc ttcagctgac gctggaatag ttgagctaaa
    42661 ttagtcatca tatctttatt ataaaataac caacagcaaa aaatacaatg aaaaacaaac
    42721 ttggaactaa tgtatcatta ataaatgttg aaaaattcag caactcatta ctgtatgatt
    42781 ttagcagccc aaccaacata taccctataa aagctaataa gatggttagg aatcctttag
    42841 aatttttact cattcataga cacttcctta aataactttt attatagaag gtattgtaaa
    42901 taataatatt gacaaaaagt gactcaaaat aaaaaaaaac ttacattata ctggaatgaa
    42961 ctgtattgat agtgtaccta aaagctctac tgacataaga tcgtgtaatc ggtaaagtaa
    43021 ttttattaat tatacgggat gtaatcaatt agagaataaa ctaatcataa aaggtacttt
    43081 tacattcata attgtcgctt tgagattcta ctttggttca ctatctgacc caattgcaca
    43141 ttttcgatca ttacaagtat tggtgtcgtt agaacaacta aataaaaact agcggtagat
    43201 ttttcagaat ctatgctaat ttctgtatgg atgttccagt gatggcaaat ttctcttttc
    43261 attgtttttc ttgtcgcatg atatgccttt ttgtgttatt cagttaaact atagaaattc
    43321 tttcctagac agatcttgat cccaaatttt acgtttttga gcatcgatat agtttaaaat
    43381 gacattactc atatctaaag agtttagatt ttttaggact aaaactgtca gttgttcttt
    43441 ttcttctttc tgttaaagta ttgtcgcgac ttttcgtata cgttaagata tgtcgtgaaa
    43501 aaacaagttt tgtgacacaa aaaaacgaat gacttgagga aatcacctca atcattcgtt
    43561 taaaatgcta tatattcttt agtttaagat acggaaatta atgccatcga ccacactagt
    43621 agaatgaacg acatttactt ggcctttttt taatttcgtt atgtttagca tataaaaata
    43681 ttatagagtt tacttacaat gcttaaaaca tatactgtat aatctgatca attttagcca
    43741 tgtcttgcaa tggtaagttt tctatttttt tgagttttcg ttccttaaaa tcaagagatt
    43801 ttaattgtgt aataattact tgtcctttta tatctagctc ttcaggtaat gagtaacgtg
    43861 tcggtaaatt ctttatagtg gaagtaattg gacatatgac cgaaaacatt gttttacggt
    43921 taaattcata acgagagacg actagtcctg gtcgtctttt ttgaatttct tttcctacgg
    43981 agggatcaaa atcaatccat acaatatcac cttttttagg aatataaatt ctttcttctg
    44041 acactataaa atttctctcc cttcaggagt taaatcttcc cattcgtctt tctcataata
    44101 ctcggcctct tccccaccac taaatggatc ttcaattttt ggaactaacg taattgttcc
    44161 atcatcagaa tacacaacga tatattcttg gttttctgaa ggtttctttc cgttatcaga
    44221 aggtaaagtt accactacag aacttccttg taatctagat tttgtaacaa gcattatcat
    44281 cactccctta ataaaagtat actatataac actaagtaac gcaaaattcc cctttgtaaa
    44341 ctatctagaa aattgttttg tttaaagtat atcttattgg atataaatag aaataaattg
    44401 tagccagttc attttttctt taactaaaaa aataatccaa gaaacattga acctatggat
    44461 ccaaaagcta cccaaatact atagctaacc ccccgctacc ttcaattttg cagataaaac
    44521 gctttgggag aaaatttcaa agacaacagg ccatcctatc atgcccgatt ataaacaaaa
    44581 agctggctat tccttttgct ctgacaaatt tcaattggga aagttcacga aactagcagg
    44641 tgaagaagtt tcaactgttc ggatcaaaga atgcccgctt caaatagaaa cattggttca
    44701 acgaatttca actagacaag actttgctat tgtagaacgt gagatcgtag ctatttttgt
    44761 agaagaaggg attttacatg atccaactca tatcaatgtg gataaatggc aacctcttat
    44821 ctataaattc agagaatata caactgccaa ccagtcgtta actttgaatt ttagattcca
    44881 agaattcaaa gagtcataat gataaacatc gttaggtata aataaaaaag actcgcagca
    44941 cgagtaaaaa acaaatagaa gctatacatt agaaaggtcg actgtgaccc tatagatgca
    45001 gtatatgcag cttatgagta tttcttctgt tctctttgtt tttgggatct gtgctgaagt
    45061 ctttttcgtt ttttgccgct ctttctttga aaaatcgatt atgttatacc ttctccatta
    45121 ctaattcagg catctgctga gtaatgattt ttcgcgcaaa agaaatatct gttacccaag
    45181 gttcgatttg ttcttcagtc aagacaagcg gcattcggtc atggattggt gcgactgtgc
    45241 ggttaggttc agtagtcatt atgattgatt ctgccatttt ttcatcctga tggctacgat
    45301 aaaaaccgcc aatatacact attttttcct tatttgaaaa cataaatttg tctttttctt
    45361 cattccactc attgggaaaa cacaacgatt ttgcaaaaat ggttgatgaa aggtcttttt
    45421 ttcttctact gtctctgcac gggcattaat catcaattgt ccttttttaa atccggtaaa
    45481 tccccacttc atgatttccg gagtgaggat gttttcttcc tcttttctta acgtcacaac
    45541 aagatttgac ggaaaaactt ctccagctgc taattctgct gcctgcttcg gttgtttcgt
    45601 ctgggcttct cgataataat tctgaaggag ctctgactct agatcaaaaa aataacgacc
    45661 acacatccta atccctccca atcttttttt ctatcataca ctgcaacaag aagttcgcac
    45721 caacaaaacg ctttcaattt ttgtagaact aaaaaagttg tgacaagtga caaactctcc
    45781 agtgaatggt attctcctgc caactcctcg gtcacaagtc acaatattca acaaacatga
    45841 gaagctgctt tttgaaattg ttccttgtga ctcggagtga aacggcaggc ttacgacctc
    45901 tgggtaaacg gtgttcaaaa gccagtttct cggtattaag ccaaatttat gcaaaaatgt
    45961 agaaagctat tttcacaaaa tttttctta
//
LOCUS       DACGLW010000011        45748 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.011, whole genome shotgun sequence.
ACCESSION   DACGLW010000011 DACGLW010000000
VERSION     DACGLW010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45748)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45748)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45748
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.011"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            101..544
                     /locus_tag="IXT53_000705"
     CDS             101..544
                     /locus_tag="IXT53_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ4904556.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(550..1734)
                     /locus_tag="IXT53_000706"
     CDS             complement(550..1734)
                     /locus_tag="IXT53_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HAQ4904557.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            2002..2700
                     /locus_tag="IXT53_000707"
     CDS             2002..2700
                     /locus_tag="IXT53_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HAQ4904558.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2707..3384
                     /gene="nth"
                     /locus_tag="IXT53_000708"
     CDS             2707..3384
                     /gene="nth"
                     /locus_tag="IXT53_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HAQ4904559.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(3510..4043)
                     /locus_tag="IXT53_000709"
     CDS             complement(3510..4043)
                     /locus_tag="IXT53_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4904560.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(4340..6721)
                     /locus_tag="IXT53_000710"
     CDS             complement(4340..6721)
                     /locus_tag="IXT53_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAQ4904561.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(6711..7358)
                     /gene="recU"
                     /locus_tag="IXT53_000711"
     CDS             complement(6711..7358)
                     /gene="recU"
                     /locus_tag="IXT53_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HAQ4904562.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            7422..7961
                     /locus_tag="IXT53_000712"
     CDS             7422..7961
                     /locus_tag="IXT53_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HAQ4904563.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            8051..8470
                     /gene="gpsB"
                     /locus_tag="IXT53_000713"
     CDS             8051..8470
                     /gene="gpsB"
                     /locus_tag="IXT53_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HAQ4904564.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            8492..8859
                     /gene="rnpB"
                     /locus_tag="IXT53_000714"
     ncRNA           8492..8859
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="IXT53_000714"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            9068..10234
                     /locus_tag="IXT53_000715"
     CDS             9068..10234
                     /locus_tag="IXT53_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HAQ4904565.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            10280..11776
                     /locus_tag="IXT53_000716"
     CDS             10280..11776
                     /locus_tag="IXT53_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HAQ4904566.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            11857..12540
                     /locus_tag="IXT53_000717"
     CDS             11857..12540
                     /locus_tag="IXT53_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ4904567.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            13185..14375
                     /locus_tag="IXT53_000718"
     CDS             13185..14375
                     /locus_tag="IXT53_000718"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HAQ4904568.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            14477..15430
                     /locus_tag="IXT53_000719"
     CDS             14477..15430
                     /locus_tag="IXT53_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HAQ4904569.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            15499..16848
                     /locus_tag="IXT53_000720"
     CDS             15499..16848
                     /locus_tag="IXT53_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ4904570.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVIAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(17031..17354)
                     /locus_tag="IXT53_000721"
     CDS             complement(17031..17354)
                     /locus_tag="IXT53_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4904571.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            17483..18646
                     /locus_tag="IXT53_000722"
     CDS             17483..18646
                     /locus_tag="IXT53_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4904572.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            18716..20065
                     /locus_tag="IXT53_000723"
     CDS             18716..20065
                     /locus_tag="IXT53_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ4904573.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(20110..20541)
                     /locus_tag="IXT53_000724"
     CDS             complement(20110..20541)
                     /locus_tag="IXT53_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ4904574.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            20929..23571
                     /gene="alaS"
                     /locus_tag="IXT53_000725"
     CDS             20929..23571
                     /gene="alaS"
                     /locus_tag="IXT53_000725"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HAQ4904575.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            23771..24367
                     /locus_tag="IXT53_000726"
     CDS             23771..24367
                     /locus_tag="IXT53_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ4904576.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            24489..25637
                     /locus_tag="IXT53_000727"
     CDS             24489..25637
                     /locus_tag="IXT53_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAQ4904577.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(25675..26088)
                     /locus_tag="IXT53_000728"
     CDS             complement(25675..26088)
                     /locus_tag="IXT53_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HAQ4904578.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            26167..26598
                     /locus_tag="IXT53_000729"
     CDS             26167..26598
                     /locus_tag="IXT53_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HAQ4904579.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(26697..26930)
                     /locus_tag="IXT53_000730"
     CDS             complement(26697..26930)
                     /locus_tag="IXT53_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HAQ4904580.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            27113..28783
                     /locus_tag="IXT53_000731"
     CDS             27113..28783
                     /locus_tag="IXT53_000731"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HAQ4904581.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            28930..29316
                     /locus_tag="IXT53_000732"
     CDS             28930..29316
                     /locus_tag="IXT53_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4904582.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            29303..30001
                     /locus_tag="IXT53_000733"
     CDS             29303..30001
                     /locus_tag="IXT53_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ4904583.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            30006..30470
                     /locus_tag="IXT53_000734"
     CDS             30006..30470
                     /locus_tag="IXT53_000734"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HAQ4904584.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            30467..31378
                     /locus_tag="IXT53_000735"
     CDS             30467..31378
                     /locus_tag="IXT53_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ4904585.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            31613..32152
                     /gene="pyrR"
                     /locus_tag="IXT53_000736"
     CDS             31613..32152
                     /gene="pyrR"
                     /locus_tag="IXT53_000736"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015694870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HAQ4904586.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            32166..33449
                     /locus_tag="IXT53_000737"
     CDS             32166..33449
                     /locus_tag="IXT53_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HAQ4904587.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            33462..34388
                     /locus_tag="IXT53_000738"
     CDS             33462..34388
                     /locus_tag="IXT53_000738"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HAQ4904588.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            34407..35687
                     /locus_tag="IXT53_000739"
     CDS             34407..35687
                     /locus_tag="IXT53_000739"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HAQ4904589.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            35692..36768
                     /gene="carA"
                     /locus_tag="IXT53_000740"
     CDS             35692..36768
                     /gene="carA"
                     /locus_tag="IXT53_000740"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HAQ4904590.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            36773..39958
                     /gene="carB"
                     /locus_tag="IXT53_000741"
     CDS             36773..39958
                     /gene="carB"
                     /locus_tag="IXT53_000741"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HAQ4904591.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            40012..40788
                     /locus_tag="IXT53_000742"
     CDS             40012..40788
                     /locus_tag="IXT53_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HAQ4904592.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            40785..41717
                     /locus_tag="IXT53_000743"
     CDS             40785..41717
                     /locus_tag="IXT53_000743"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HAQ4904593.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            41714..42430
                     /gene="pyrF"
                     /locus_tag="IXT53_000744"
     CDS             41714..42430
                     /gene="pyrF"
                     /locus_tag="IXT53_000744"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HAQ4904594.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            42437..43066
                     /locus_tag="IXT53_000745"
     CDS             42437..43066
                     /locus_tag="IXT53_000745"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HAQ4904595.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQI"
     gene            43054..43368
                     /locus_tag="IXT53_000746"
     CDS             43054..43368
                     /locus_tag="IXT53_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4904596.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            43406..44047
                     /locus_tag="IXT53_000747"
     CDS             43406..44047
                     /locus_tag="IXT53_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ4904597.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            44078..44782
                     /locus_tag="IXT53_000748"
     CDS             44078..44782
                     /locus_tag="IXT53_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HAQ4904598.1"
                     /translation="MKKNMDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKG
                     EETKIEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSE
                     HIINGKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVN
                     PDVFFPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPI
                     QPLNGREIYYFEEFEQ"
     gene            44779..45681
                     /locus_tag="IXT53_000749"
     CDS             44779..45681
                     /locus_tag="IXT53_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ4904599.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
BASE COUNT        15005 a         7997 c         9883 g        12863 t
ORIGIN      
        1 tttttcttgg tttcagattg ggttttccta caaaatattt ttatctttgt tgaataggaa
       61 acaaaaaaac aatataatga aagaaaaaga aggtgtagaa atggataaaa gacaactgct
      121 aatagaagaa tatctggatt tagctaatga gattattcat cgtaataaac cagaaatgga
      181 aaaagaattt gcaggattta ctctaaatga actagaagtg attgaacata tcgggaaaat
      241 ccctgatcct aacgtgacaa aattagcgaa tgctagttac atgactagag gagccatcag
      301 taagctaaca aaaaaactga tagctagagg gatcataaac acttatcaaa gcgaagagaa
      361 taaaaaagaa atctattttc aattgacgcc aatcggacaa aaaataagga atagacacga
      421 gcaactccat caaaaatggg tacagcgaga tgctactgta tttgaaaata tgtcaaaaga
      481 agaatttgac acagtatttc gttttatcgg tcgatacaga gaatttatac gaagtagcaa
      541 atgaagctac tattttttat tgtagtgata ataggcaaaa atcataagca aaaatccgaa
      601 taccgtcgga agaaatggtg ctgcagcata aagctgtcct gcgattagcg ggccgatgac
      661 gcgcgataga gattgtatac tttgggtacc gcccattaca agaccttgct ggttatctga
      721 gacagaattg gatagttggc cattgaatac aggagtaaaa atagaatcac cgaaaccata
      781 acagataatc ccaaataaaa acaaaatcgg cagtagaaga attccagata atgtgatgaa
      841 tagataggcg aggatttcac tgaacatccc tattcttgtg atctgctgtt ctgaaaattt
      901 tttgagcaat cgaggcataa tgaacaattg cgagacagta tccaagattc caatcaaaga
      961 gaagcttaaa ccaaccaacc cagccttcca ctgaaaggcg tcaatcgaaa attgagaaaa
     1021 gatagattga agagagccag ctgccaacca aacagcaaaa cctgctgtca ataaaggaat
     1081 aacggatgaa atcttgaata attgttgtaa ttggtgaaaa ggtcttacat gggagaaact
     1141 taaatctact gaacgttttt ttattggaag gctttctagc ataaatgtgt agccataaac
     1201 ggcatttaag aaagtaaaga gtgctccaat aaatatagga acagaattcc ccaactcagc
     1261 taatagaccc cctataatag gacccagcgt tgttcctata ccgactaaag cacccatcca
     1321 tccaaaaact ttggttcttt cattaggttc cgtgatgtcg gcaaagtaag catataaggt
     1381 ggagatttct cctgccgtca atccttcaat gatacgacca aggaacaaca tccatatcga
     1441 atttcctaaa ccaaaaagca aatatccgat acttgatcct aataaactga taataaggat
     1501 cggatgacgc ccaaaatgat cacttaagct accgagaatg ggggcagata agaaggcagc
     1561 tagagcataa acagacatga gcaaagtgat atagaaagct tgctgatgta tggagtgaga
     1621 ataaggcagc gttaaaaacg gaagtacagg actgacgata gtaaatccta atccagtgaa
     1681 taagacagag acgtatccaa aaactttaga atgtttattt gtgaaactgt acatgtaaaa
     1741 actcctttat gcaataatgt taacttggaa acaaaaatag tgtatagtaa ttttgtttcc
     1801 ttgtcaacat tctaaggaac agcgaaatct acttaattta tttattccga aaagtgaaga
     1861 tacgcatcaa aaacatgaac aaacaaaaat caagtagacg acattgtaaa tctgaccgat
     1921 cagtgaagta aatagaaaag aaacctgttc ttgcttccca tgtttttttt acgtatactg
     1981 tgattgagcg gaggagaaat catgttgaat ttagaaaatt acttaagcgc tggacaaaca
     2041 gtcatatcta atcttttact taaaaattat cataggatcg gtctacaatc tgaagaattt
     2101 ttatttgttc tacagttgca tatggcacag cttgaaggag atactttccc tgatttgcag
     2161 atgattgcta tggatatggg aatcaaacaa gatcagattt ttcaaatact ggatcgtctc
     2221 gtaacaaatg gatttatcaa gatagaaacg acgattgtca acggtaaaaa agcagatcga
     2281 tataatcttt accccatcta tgatctatta ggtgagtatt taaaaacaca agaaaaaaaa
     2341 tacgaagaaa aaacacaaga aaaagaaatc cagtcagtgt atcaattatt tgaacaagaa
     2401 tttggacgcc cgctttcttc gatcgagttc cagcggatcg gacaatggct agaagaagac
     2461 cattatcagc cggaaatttt gaagctagca ctgcgggaag ccgtattaaa tcaggcttat
     2521 agttttaatt acgttgaccg cattttgcta tcgtgggaaa gaaaaaatct acgtacaaaa
     2581 caacaagtgg aagaagacca aaaacgaaga aaacagcagc ttcttcaaag agaagtagaa
     2641 cagtcagctc aacaagaaga attgccgaaa gtctcactga aaaattggtt ggaggagtag
     2701 cggaatatgt taagcaaaca aaaaacaatg gaagctctag aaacgatgta tggaatgttt
     2761 cccgaagcac atggagaact caagcacaat aatccatttg agttattgat tgcagtgatc
     2821 ttaagtgccc aggcgacaga tgtatcagtg aataaagcaa cgccagattt gtttgcctct
     2881 tttccgacac ccgatgcgtt agcagaagct tcaatcgatg aaatcattct taaaataaaa
     2941 acgattggtt tgtatcgaaa taaagcaaaa aacatcaaag cgtgtgcaca gcagctgatt
     3001 gaacgtttcg atgggcaagt gccaacgtca agagaagaat tgatgtctct tcctggtgta
     3061 ggcagaaaga cagcaaatgt cgtattgggg gatgcattcg ggataccagc aatcgcagta
     3121 gatacccacg tggaacgtgt ctcaaaacgc ctgaggatct gcaaactaga tgctacggtc
     3181 atggaagtag aagaaacact gatgcggaaa gtaccacaag aattatgggt aaagacacat
     3241 catacactga ttttttttgg tagatatcat tgcacggcaa gaaatcccaa gtgcgaggta
     3301 tgtcctttgc tatcaatatg tcaagatggg aaaaatcgga tgcgtctaaa agaaaaggcg
     3361 ctaaaaaaga aaccacgcct gtagacgtgg tttctttaat tgcaaataaa tgtatgaatt
     3421 atcttgttat tatggagaaa agctgtttcg tttatctttt ttgcatgaaa aagtaaatgt
     3481 acatactcag tagctaggat ggtaacttat tacaatttat tgcgataaaa ataaaagttg
     3541 ttatttttat tgggaaaata cccgatgtag aaaataatgc cttccaatat acctataaaa
     3601 taagtccagt attccaaagg attatataaa taagtgacgt atctatctaa aggtgtaata
     3661 aaaatgataa gtgataataa aaaaatacca tttataacta aacataaact aatggatttg
     3721 ggcggtttca ggctcatcag aaaaatatag attctggtga acaacaacca taaaaaagtg
     3781 agaatcattg catgagagct tctttccata aattttcctt taagagtagt gcttaaaagt
     3841 acagaaaaaa taataaatgc aaggatagga gcaattaaag taaatttatt taataataaa
     3901 caaaattttt cagctttttt attcttggaa taacgaggta ttgttttcga taaaaaaaga
     3961 tggtgcagac aaattatgat aattaagcat actatcagat aactcaaaat gaagccttct
     4021 ttcaaaaaat tattcaaaac catatattgt attttttatt tgataataac aatgggatta
     4081 ttaagaagtg gcactaaata aaagactagt gtgtatttag ccgatttaat tataaacaag
     4141 taaatggata aagtatatgt aatgataaaa gaaaaatgga ttttctaatt tttaagctaa
     4201 atgttagatc acacatgtat atgctggata gtcttataaa aaatcatgta atacagcttg
     4261 gtacatgatt tttatttgta gctgtttttt acatagacac aaaaaagaaa ccacgcttgt
     4321 agacgtggtt tcttttttac tatctgtttc cagaagctga ggtgctgctg gatggtgtat
     4381 ggtcatttgc agcatccgct ccgctagttg ctggctcact ggaagaagca ggaggagcag
     4441 atgaagacgg tgtcgagctt tcttcggatg aagaagaagg aaccgttgtt gaagacgctg
     4501 gctcgcttgt ttctgctgtc gtgctttctt ttgaggactc agcagttgat tcgctttgac
     4561 tagtagagct agagctttga gtcgttgtct ctgttgttga gcttccagga gtctggacat
     4621 agctggaaga tggaatagtt gttgatggtg taattgcatt cgatctagct gtatattgat
     4681 ctttgtagta cagttcaccg cccacacgaa ttaatccgct tggcatctcc cagtctgtat
     4741 ttgtcacgtt agctgaaaca tactgcataa gctcgcgata aacatcagag gctacgtgtg
     4801 acgattctga agtgacaggt gtcatttttt tattgtaacc tgtccacacg gaaatcgaat
     4861 aatttggcgt atagcccgca aataggatat ctggatacac cccagaagaa ataccaagtt
     4921 ttgcatactc gtcatctgtg taattggatg ttccggtctt accggcttga tataacccag
     4981 cgatttgcgc attcgtacca gttccttcag tgatcgtatc ttttaaaatg tctgtgatca
     5041 tataagctgt ttctggagac atggctgttt ttccatctgg ctcataagtt tcttctgttc
     5101 cgtcttggaa gacgatttta ttaacatatt gaggtttgta ataagtccct ccattggcga
     5161 aagccgcata ggctgctgcc atttttaagg aagaagctcc gtattttgtc ccatcttgtt
     5221 cttccgtatt actagagatc gcattcgatt gatggatcgt actgtattcg atcccaagat
     5281 tttttaagaa agaagccact ttatctgaac cgacttcatt gaataatttg actgctggaa
     5341 cattacggga taagtaaagt gcttggcgta aagtaatggt gcccatatat tgattatccc
     5401 agtttcctac aggagtcgaa gtcccttcat agttatacgg tgcgtcagta atagtttttc
     5461 cagtagaata tttcaaatat tcaaaagctg gtccgtaatc tgtgacaggt ttcatcgtag
     5521 aaccaaaatc acgggaagta ttcactgcta agttgttgcc taaagttacg tcttctgcga
     5581 tgtgacgtcc gccaatctgg gcttttactt tcccggtatt ggtgtcgata agggtggagg
     5641 caacttgcat ctcgtcgtct gggtaagaga catactgatc cgtgttgacg atatcatata
     5701 gtttcttttg ggcgtccaga tccaaattcg tataaatctc taaaccgtct gtgtagacat
     5761 tcttatctgt tttttcttgt acttcattga tgacttcttt gacataatta tcgacgatct
     5821 tagtattgtc atcggattga gttagttctt gcagaccatc cgttaccggt acattaacag
     5881 cttgatcata ttctgtttga gagatttttt cgttttgaag catcgtatac aaaacagtat
     5941 ctcgtctttt cttcgcctga tctggataaa cataaggatc ataggcggaa ggggcttgag
     6001 gcatcccagc aagcaatgct gtttgcggta aagaaagttc tgataatttc ttaccaaagt
     6061 acatctcaga agctgtctcc atcccgtaca agccgtttga catatacact ttatttacgt
     6121 agtaggtcaa gatctcttgt ttcgattttt tttgttccaa tcttacagcc atccacgctt
     6181 cttgggcctt gcgtttcaga gtctgatcct cggcactggt ggagaagaag gaaagtttga
     6241 tcaattgctg tgtcaaagta cttccgcctt gcagtccgcc agaggtaaag ttagagaggg
     6301 cagaaccgat gatccgaata gggtcaaccc cgatgtgttt ataaaaacgg cgatcttcta
     6361 ccgacacgat cgcatcttct aatgttttcg gtaattcatt cgcagaaatt ttttcacgct
     6421 tttctgcgcc taaatcttcg aatagttcgc catcttgtgt atatagtttg gaagatacgg
     6481 tagcgtctaa ttttttatcc gtcaattcgg gcgcatcttt tgcgtaatac cagaaaagtc
     6541 ctacacctgc taaaaagagg atacagaaaa atgagagtat tcctaaaagg atcttgataa
     6601 ataatccttt tttatgtgtt ccagaggact ttccagaacc tttgccagaa ttcttcttca
     6661 cactcttttt aggagtaggc tgtttctgtc gtctagagct tctagtttgt tcatttgcca
     6721 ttcttgcttt ctccttcaat agataatgtt gataaataga agtcaaccgc gtccaaataa
     6781 ggaatccgcg gagaaatacc agttttgatc tctattccta gttcttcgat caaagataaa
     6841 ggtagtgatt tgcgtccttt ttccttttgg gcgtcccagt aagcaatcaa atgaacacta
     6901 ttcaaataaa aacagcgctt caaagacgaa aaccataaca agacaaagca gaccccattt
     6961 tgttcgaggc atgctttcat atgttcgatt tgatgggcat ggaagttttt aaagggaaaa
     7021 gatgttttgt tcttggtttc ttttgcttca aagtccaagt aaaagcctcg atagacgcca
     7081 ttatagtctg tagttgaggc ttgggtaaag taggcttcct tgatcacagc agcacttctc
     7141 ttaggatagt ctactttcac gatttggata ggggtaggct ttttatggat aaccgccaac
     7201 ccgcgggaaa gataatattt attactttca ttgattgctt cttcaaagcg catcccccga
     7261 ttgccgaatt cagtgatctg atttcttttt tcttgaattt tttcgacggg agaggcattc
     7321 ttataataag gttggccatt tggatagcgc aataccatct ttaatcacac tcctaggcca
     7381 cattatagca aaaaagcgga tagaaagaaa aggaatctga gatggataag tcaaaagtaa
     7441 tctatgtgtc tgggtatcga agtttcgaac tcggtgtttt taaagaaaat gatcctaaaa
     7501 tccaagttgt taaaaatgtt ttaaaaaaag aactccatca gttagcagaa gaagggctgg
     7561 aatgggtact tgtcagtggg aatttgggcg tcgaactatg ggctgtgcaa atagtagcgg
     7621 aattgaaaga agaatatccg gaactatctc ttggtatttt gtatcctttt tctgattttg
     7681 gtaataactg gaatgaaggg aaccaagaaa aaaaacaatt agcagaacaa ttagcagatt
     7741 atgtcgaagc tgttagccat caaccctacc aatcacctag tcagctaaag aatcacactc
     7801 gttttatact tgagcataca gtgggctgtc tgctgatcta tgatgaagaa ttccctggaa
     7861 aaacgaagta tttttttgaa gatgcacaga attaccaagc agatcatccc tacgaaattc
     7921 ggctgatcag tatggacgat cttcaaaatt ttagtgaata atcgatattg tgtttgattt
     7981 atcaactttt ttcctataca atgaaaaagg tagaaatgtt attacgtaaa tgaaaactag
     8041 aggtgtaaat atggcgaatt tggtttatag tcctaaagac attttacaac aagaattcaa
     8101 aacaaaaatg cgcggctatg atcccgttga agttgatgaa ttcttagaca atatcatcaa
     8161 agattatgaa acttacagca aagaactcct tgctttacaa gaagaaaacg atcgattgag
     8221 tgcaaaagta gctcaattat ctaaaactca aggagcagct caaacacgcg tgcaacaaac
     8281 agaggcacca aagagcgcag cagtgaccaa ttttgatatc ttaaaacgct tgtcaaatct
     8341 tgaacgtgaa gtatttggta aaaagcttga tcaacaagca agtgcagtca aaccagcaca
     8401 acctaatcca aataactaca caaatgcaga cacaagctta gatgataatg aaaaaacacg
     8461 tcaattttaa tttttgatta atgaatttgc ggttttcggg taatcgcggt ctgacttgat
     8521 caggctgagg aaagtccatg ctcgcacagg ctgtgatgcc tgtagtgttc gtgcttagcg
     8581 aaaccataag ctaaggtact ctttggagta acggcaggaa aacaagctaa ggcttcggct
     8641 atgcttaagt atccttgaaa gtgccacagt gacgaagcgg ctggggaaac ccatccggtg
     8701 gaacgcggta aacccctcga gcgagcaacc caaacaatgg taggggcgct ttttgtctgg
     8761 aagaagaaca ggacaaaaag gcagtataac tgcagataga tggttaccgc tttgtttttt
     8821 ctccctggaa aagcaaagta cagaacatgg cttatagaaa accgcaaaca tcagggaatt
     8881 aggtcgtgac aaaagtaatg cgttataaga aacaagaaaa acatttgaaa gctatttttc
     8941 aatacttcag atgaatcgtt cttattacaa aacaaacttt tgtatcaccg gttattcgga
     9001 atttgagtaa aagtgtgggg taggactgat gtcccacctc ctttttttat aaataaggag
     9061 attgtgtatg gaaaaaaaca ggaagttcaa cctcgtcgca actgcggcaa gcgggctaga
     9121 agcactggtt ggtaaagaat taagagatct tggaatcgaa tgccaagttg agaacggacg
     9181 tgcacgtttt gaaggaacaa tggaaacgat cgctacagct aatttatggt tacgtacagc
     9241 agatcgagta aaaatcgtgg ttggggaatt cgatgcgtat acgtttgatg aattgtttga
     9301 aaacgtaaaa gcattgcctt gggaagattt tcttccacta gatgcagctt ttcctgtagc
     9361 tggaaaatcg atcaagtcga aactatatag cacacctgat tgccaagcga tcacaaaaaa
     9421 agcaatcgtt gaacgtctcc gtacctatta tcaccgtcca gcttctgtac cactgacgga
     9481 aactggcgca cattttcagt tggaagtagc tttgctgaaa gaccatgtga tggtaactct
     9541 ggatacgaca ggaccaagtt tattcaaacg tggttatcgc ttagaaaaag gtggagcacc
     9601 attgaaagaa aacatggcag cagcacttat tatgttgaca aattggcgaa aagaccgtcc
     9661 attttatgat cctgtatgtg gttcaggtac gctttgtatt gaagcagcct taataggcca
     9721 taatatcgca ccaggattca atcgtgagtt tgcttgcgaa tcttgggatt ggttcagtca
     9781 agaaatcatg gaaaatgttc gatcagcagc cgaagaaaaa gcagattatg atattgaatt
     9841 agatattact ggttcagata tcaatggacg gatgattgaa attgccaaag ccaatgcaga
     9901 agaaatcgga ttaggctctt cgattacctt taagcaacaa gcagtcaaag acttcaaaac
     9961 ggataaagaa tacggggtca tcgtggctaa tccgccttac ggagaacgtc taggagaaga
    10021 agaaactgtt cggaaattat ataaagaaat gggcgatgta ttccgtccat taaaaacatg
    10081 gagtaaatat atcttgacta gcgacttagc gtttgaggaa ttttacggac aaaaagcaac
    10141 caaaaaaaga aaattgtaca atggtgcatt gcgtacggat ctattccagt attggggaac
    10201 tcgtccgcct agaaaaaaag agaaagacga ataatcttac taattgattc attcaattgg
    10261 atgtttggag gaatagggaa tgaaagaaaa agagttttta caagaagtaa aagaaatcaa
    10321 tcttctacaa caagctttag gaatcctcga ttgggataca caaacaggta tgcctgaaaa
    10381 agctagtgca taccgaagtg aagtagacag ctatctttac agcatttatt tttcaaaaaa
    10441 aatcggtcca aaaatcaaag aagccattcg ctatttttct gaacatccgg aagaattgtc
    10501 ggaagttgga actgccgttt ttgccaaagt aaaagaagag tatgatttgg aacatcgcgt
    10561 accggaagat cttatgagaa aatatacagc tgccgtttct tcagcacacg gacaatggca
    10621 aaaagctaga gaaacacaac aattttctga ttttcaacac gcattgaatc aaaatattga
    10681 attaacaaaa cagttgatac cttactggag aaaagatgaa aagacgaatt atgatgtttt
    10741 gctgaatcaa tacgaacccg ggatgactgt cgaaatcctt gataaggtat ttgatcagct
    10801 taaagaagga attctttcga tccgccgtac gctccaagaa aaagggactg aacctgaaac
    10861 agacttttta aatcggagaa tgacgaaaaa acaacagaag agatttgtag tgaaagtaat
    10921 cgaacaactt ggttatgatt ttagtcgtgg tcgtttagat gacactgtcc acccatttat
    10981 gactggaatt aatccgaatg atgcacgaat cacgacacga tggaacgaaa cagatttcaa
    11041 aatggctgtt tttggagtga tccacgaggc tggtcatggt atgtatgaac aagacatcga
    11101 tgataaatat gcgtatacac cggtttatga aggtacatca atgggtatcc atgaatcaca
    11161 atcgttgttt aacgagatca ttattggaag tagtcgcagc ttctggaaaa agcaatatcc
    11221 tttctttcaa gaatgtgcag aaggaacatt cgatgatatt ccttttgaca aattttatcg
    11281 ttcattgaac catacaaaat caagtttggt tcgtatcgag gcagacagtt tgacttaccc
    11341 tctccatatc atcattcgtt atgaaatcga aaaaatgatt ttcaatgaag gggtagatgt
    11401 tgctgaacta cctgaaatat ggaatcaaaa atatgaagag tatctgggaa tacgaccaac
    11461 aaatgatgtg gaaggcatcc tacaagacgt tcattggtca ggtgcaagct ttggttattt
    11521 tccctcttat gcattagggt acatgtatgc ggcacaatta cttcattctt tgcagcaaga
    11581 aatagatgtg gaaaatgttc tagcgtcaga cgattattct ccaatcaaaa actggatgtc
    11641 agagcatatc catcaatatg gtgcatctag aaaaccaaat caattaatca tggatgcaac
    11701 acatgaaccg cttaatccgc aatacctgat cgactatatg aaagacctgt attttaatgt
    11761 atatcaggta aaataaaata caatttacaa agatagctga aattcatcta atctatagat
    11821 gttttcagct atttacttaa gaaagaagtg gaacgaatgg ataaattaca agtgatcgct
    11881 gattatagta tggaccaatt agcgcatgat caaacaggac atgggagcga tcataccaaa
    11941 agagttgtca agttagctga aaaaattttg gagacggaac cgcaggcaga ccgctttgtg
    12001 acgcttgcag cagcttacct acatgatacg attgatgata aagtcgttaa aaatgagaac
    12061 gaggcaaagc aacagcttcg tgtattcctt cgctctttgc caatagtaga ggaacagatc
    12121 tcgatgattt tttccattat cgaaaatatg tccttttcta aaaatctctc tgaagcggcc
    12181 gaactatctc tggaaggtaa gatcgttcaa gacgctgatc gtatcgaagc attaggagca
    12241 atcggcattt tgcgtactgc ttatttcggc ggtggacatg ggcacccgat ttttgatcct
    12301 gaactgtatc cgcaaacatt caaggacaaa gaaaattacc gaaaaggcac gacagtgatc
    12361 aatcattttt atgaaaaact gttcttgctg tctgataaaa tgaatactga ttacggatat
    12421 gaagaagcca aacgaagaga aaccttcatg cgtgaattct tagaagaatt ttttctggaa
    12481 tggtctattg acgatgaaaa ttttactcca gacaattgga tcaaaaataa caaaaaatga
    12541 tggaggttgt gacagaagtg gtcagctcca agatatttcg gatcattttc cgtaaaagtt
    12601 acttctggaa cactgtttac caagaggtcg tgagcctgcc gttcagctcc gagtcacaag
    12661 gaacaatttc aaaaaatagc ttctcgtgtt tgatgaatat tgtaacttgt gattcggaat
    12721 tggcaggaga acaccgttta ttcggaagaa taggactgtg acaagacttt tgtcacagtc
    12781 cttttttcga attcaacaag taaaactttt aagaaatatt cagagagtaa aattaaaaat
    12841 tgctgataaa cgaagatgga aattttatgt atttttattg gtctatgcaa taaataaatt
    12901 aaatattcag aatacaattg attttaacga aagggcttct atcaatggtt gtgttgtatt
    12961 tatctgccca ggtagagtaa aatagatcat tatctaaaag gtaattgttc atactatata
    13021 agtcatttga aagtaaaaag atactgtaaa taaaaaatgt ggttaaataa ttctcataat
    13081 cagtgtaagc gttatcaaaa atatagcata cttgctttgt agatgaatga cacgatatat
    13141 gtttggtatg ttctgcaaga cagatgaata tggaggtgga gacaatgtct tccaaagaac
    13201 taacaatgta tttagagaaa gaattggatc aactgaaaga aaaggggtta tacaatacga
    13261 tcgacatact agaaagtgaa aatggtgctt gtatcatcgt agacggaaaa aagatgatca
    13321 atctagcatc caacaattat cttggatttg ccaatcgcga agaattgaaa aaagcatgta
    13381 ttgaagcaac cgaaacttat ggtgtaggag caggagcagt ccgaacgatc aatggttcgc
    13441 tgaaaatcca tcagcaatta gaagaaaaaa tcgcagaatt caaagggaca gaagcagcaa
    13501 tcgcctttca atctggattc aactgcaata tgggcgctat ctctgcagta atgacaaaag
    13561 aagatgctat cttatcggat gaattaaacc atgcatctat tattgacggt tgccgcttat
    13621 ccggagcaaa aatcatccga attaaacatc aagatatgaa agaccttgag aaaaaagcaa
    13681 aggaagcgat agaaagtaag aaatataaaa aaatcatgta tatcacagat ggtgttttct
    13741 ccatggatgg agacattgca cgattgccgg aaattattcc gattgttgaa aaatatggac
    13801 tgatcaccta cgtcgatgat gcgcacggct caggcgtaac tggaaaaggt gcaggaaccg
    13861 tcaagcattt tggattaagc gacaaaatcg atatgcaaat gggcacgtta tcgaaagcta
    13921 taggtgttgt tgggggttat gtagctggat cgaaaacact aatcgactgg ttgaaagcta
    13981 gaagtcggcc attcctattt tctacttcgc taactccagg tgcagctgca gctgcattgg
    14041 cttcgattac tttgatgcaa gaacatccgg aacttgtcga aaaagtttgg gaaaatgcta
    14101 attattttaa agaagaattg aaaaaagtag gctataacat cggtatgtct gaaacaccta
    14161 ttactccagt cattttggga gacgaaaaag tgactcagac tttctctaag aagctgatcg
    14221 aacatgggat ttatgctaaa ccaatcgttt atccaactgt gcctcttggg acaggcagga
    14281 tcagaaatat gccgactgcg gaacatacga gggaaatgct tgatgaagca gtagcagttt
    14341 atcaaaaaat aggaaaagaa atggaaatta tttaactaaa tttagttttt tcgtctattt
    14401 aaaattagag aacatgttca cgcatatatt gttaatcatc tgaagatagt agtaaaaaag
    14461 atcagggagg atcatcatga aaaaagtatt ggtgacagga tgtttaggac aaattggttc
    14521 agagttgatc acacggctac gtcaagaact aggcagtgaa aatgtcattg cgacagatat
    14581 acgcatgcct gaagtcattg agaaaaacga tatctttgag atcctagatg taatggatta
    14641 cgaaaaaatg cgcagcatag cagaacaata tcaagtggac acattgattc atttggctgc
    14701 tcttttgtca gctgttgcag aagcaaaacc acaattagcc tggaatttga acgtgactgg
    14761 gctggtcaat gcattggaag tcgctagaga attccagctg aaatttttta cgccaagttc
    14821 aatcggcgcg tttggtccaa atactccaaa agatcatacc ccacaagata ctcttcagcg
    14881 tcctacaacc atgtatgggg taacgaaggt agctggagaa ctactatgtg attattatta
    14941 tgtgaaatat ggcgtagata ctcgtggtgt ccgtttccct ggattgattt cttatgcaac
    15001 cttgccagga ggcggtacga cagactacgc agtcgatatt tattattcag caatcaaaga
    15061 aggaaagtac actagtttca ttgatcaagg tacatcaatg gatatgatgt atatgccaga
    15121 tgcgatcgat gccattataa aactcatgaa tgcagatcct gatcgattgg ttcatcgaaa
    15181 tgccttcaat atatcggcga tgtcttttga cccggaagag atcaaagcat ctattcaaaa
    15241 aatcatacca gattttgaaa tggattatga tgtggatcct gtacgtcagt caatagcaga
    15301 atcttggcca aattcactag atgtttcttg tgcgcaagag gaatggggct tttcgccgaa
    15361 atacgactta gatgtgatga caaaggatat gttggaaaaa ctgaaagaaa aattggtaga
    15421 gacggtataa aacaagaacc acatccaaaa agttttttat acttttggga ttggctatgg
    15481 tatgaaaggg gacaagggat ggaagaatat caagccacgc cagtaaaaga agtaaaaata
    15541 gaaaatgaat taaaacgtac gatgggattt tttactgctt tatcgactgt tatgggcaca
    15601 gtgatcggtg caggggtatt ttttaaagcc gcaagcgtag cagaagttac aggatccgcc
    15661 agtttgcata tgttttcttg gtttctcggt ggtatgattt ctgtctgtgc aggactaaca
    15721 ggagctgaac tggcagctgc tattccagaa acaggaggga tgattaaata tatcgaacgg
    15781 atttacggaa atacagcagc ttttttattg ggctgggcgc aagtcgtgat ttattttcca
    15841 gcaaatgtag cagccttgtc gatcattttc ggtacacagt tcgtcaatct atttggtctt
    15901 tcccagtcaa tgattgttcc cgtagctgtt acagctgctg tatcgatcct gttgatcaat
    15961 tttttaggtt ctaaagctgg aggggctttt cagtcgatta cgcttgtctg caagcttatt
    16021 cctttgtttg tgatcgtcat tttcggtttg ttccgacaag aaggagtaga cttccaactg
    16081 tttccgatcc aagcaggaga aaatctttca tttttctcag ccttgggagc gggcttgcta
    16141 gcaacgatgt tcgcatacga cggttggata cacgtaggaa atatttcggg agaattgaaa
    16201 aagccggcga aagatttgcc aaaagcaatt tctttaggaa tcatcggaat catgatcgtt
    16261 tatttattag taaatgcggt gttcttaaga accgcatcta tcgacggagt cgcaggaaac
    16321 agcaatgcgg caagtgacgt tgcaaaaatg atttttggcg gcttcggtgg aagactggtc
    16381 acagttggta tcttgatttc tgtttatgga accatcaatg gctataccct tactggaatg
    16441 cgtctccctt atgttattgc aaaagaaaac aacctgcctt ttagcaagct ctttgctaaa
    16501 cttcatgata aaactaaagt tcctgtagcc gcaggtatct tagagctagt catcgctatt
    16561 ggcatgatga tgatcggtgg ttttgataca ctaacggata tgctgatctt tgtcatttgg
    16621 attttttata cgatggtctt tgttggtgtc attcttcttc gtaaaaaaga acctgatttg
    16681 tttcgaccat acaaagttcc aatgtatccg tttattccac tagtagcaat cattggaggg
    16741 acttttatcg tatcaagcac gcttatcact cagacctttt tagcttcgat ggggatagta
    16801 ttgacacttg ctggtatccc gatttatctc tatttaaaac gtagatagct aaaacattcg
    16861 aattttatta gaactgcact tctctgccaa acaaaatatt ttatttaaat gaggagtacg
    16921 tggaagaaat gtcaaaagta ctcgttaatt ttgatcacgt aaatgtcgtt ttgtctacga
    16981 tgaaatagca aagtaaaaga cagaaatcgg gctcaaccga tttctgtctt ttactttgat
    17041 ttgcgtaact gaactattcg gcgtacgccg cgataaatat ttaataaaac caagcaacca
    17101 gaaatgaaaa tgggaagcca aatcagatag acagggtagc caagcacttt agacaaagca
    17161 tgtggagtca ttttatccat ttcaagaagt gcttgctgcc aagtctgaag attgaagact
    17221 agcatgaaac ccgaaataat caggagaata aatcctaaat aatgaattgt agttcttttt
    17281 cttacataag cgatcgtagc ataaaggaac agtaaaaaaa taggtacgcc aaaaacaaag
    17341 tacatattca gcatctcgac accccctttg tgtttatctt actttacttt gaaagaaatg
    17401 agaaataata gcctcgttac ttgcttgaaa cagcaaagga ctgctaagat aagagagata
    17461 caaagaatca aggaggagtc atatggcaag ttatatcgaa ccgattcgtt ttgcgctgat
    17521 tgtttttcca tttttagcct tagcgatctc atctgttttt tttatttatg aatatcgaaa
    17581 gtatggaaca tttctagtaa caagggcagt tattttgtat tctttcgttt tttatttatt
    17641 gtgtgcgtat tttttggtga tcttaccact tccggcaaag gagacggttg cccaaatgac
    17701 agggccaaag atggagctgc atttatttgc ttcatggcaa catttcagag acaacacggt
    17761 attggtcttg tctgaaccta gtacctacct gccggcaatg aagcagagtg tatttttgga
    17821 accagctttt aatgttttat tactcatacc atttggcatt tatttaagat attatttccg
    17881 gctttctttt atgaaaacga tgattgctag tttctgcctt tctctatttt ttgaattgac
    17941 gcagctaagt ggactttatt ttatctaccc aagaccatac cgcttatttg atgtgaatga
    18001 tttagtgaca aatacattgg gtggaatcat tggttttgtc gttacgccgt tatttacatt
    18061 tatgcttcct acaagaagac gaatggatga actttcttat gaaaaaggaa agactgtctc
    18121 attgactcga cgcttggtcg cttatttgat cgactggaca tttttatctc ttattaattt
    18181 ggtagcggcg attttcttta aaattgcgtt cgattcaggg gcagttactt ctcatatctg
    18241 gtggctttta ttagaagtag gggtatactt tatgattgta ccgtttataa ccaatggatg
    18301 gacacttggc aaaaaaattg tgcgtatccg tttggtggaa gaagaccgcg aacgaatttc
    18361 tttaaaagca ttgatgatcc gctacgggta tctgtacttt cttttttatg gtttaagctg
    18421 gttggggcag ttgcttcagc aaatcatgaa ttcgacaacg aatacggtgt tgcagatgag
    18481 tgcagtattc atttctctcg gtgtcttgtt actacaaggt atttttattt taaatatctt
    18541 gctatcattt ttgagaaaaa aacggattct gttttatgaa aaagctagtc acacatatac
    18601 tgttagcacg attggccaaa caaaggatga tgattctcga aattagctga gcaaaaacgc
    18661 tttgtctgtg ttataatgcc aaagtcgtga taatttaaag agaagaggtg cacgaatgaa
    18721 taattttgat atattccgct tcaaaccgtt tatcaatgaa gcgttaaccg caaagggctt
    18781 caaacagccc acagaggtcc aggaacgttt gattccttta attgcaaaag gaaaaaatat
    18841 cattgggcaa tctcaaactg gtacaggaaa aacacatacg tttttgttgc ctttgtttga
    18901 aaaaatcaat gcagaaaaag aggaagtaca agttgttatt acagctccaa gtagggaact
    18961 agctgcacag atctatgaag cagctacaca aatcgctaaa ttttctacac cggagatccg
    19021 tgtctcgaat tttgtcggcg gtacagacaa acagcgtcaa ttaaatcgtc tgcatcacca
    19081 acagccacag gttgtagtag gcacgcctgg tagaatatta gacatgatga atgaacaagc
    19141 gctgaagatt cacaccgcat ttgcatttgt tgtcgatgaa gcggatatga cattggatat
    19201 gggtttttta aatgaagtag accaaattgc aagtcgatta ccagaaaagc tgcagttttt
    19261 agttttttct gccactattc cagaaaaact tcgtccattc ttacgaaaat acatggaaaa
    19321 tccaatcatt gaagaaatca aaccaaaagc agttatttct gaagacattg aaaattggct
    19381 gatttcaaca aaaggtaaag atgagaatca aatgatttat caactgctga caattggtca
    19441 tccttattta gcaatcgtat ttgccaatac aaaacagcat gtggatgaaa ttgccgatta
    19501 cttgaaaaac caaggcttga aagtagcaaa gattcatgga gacatcaccc caagagagag
    19561 aaaacgagtg atgcgacaag tacagaatct ggagtatcaa tatgttgtag cgactgattt
    19621 agctgctaga gggatcgata tcgaaggtgt ctctcatgtt attaatgcag aaattccaga
    19681 agatctagac ttctttatcc atcgtgtcgg acgtactgga cgtaatggct taaaaggaac
    19741 ggccattacg ttatattcgc caaatgatga gcaagccatt gatgcgatag aaaaattggg
    19801 cgtctcattt gaaccaaaag aaatcaaaaa cggggaaatc gtcaaaacat acgatcgtaa
    19861 tagacgtgcc aaacgagaaa aatcaaaaga tgtattagat cccacgctga ttggtcttgt
    19921 gaagaagaaa aagaaaaaga tcaagcctgg ttataagaaa aaaatcgatt gggcaattgc
    19981 agaaaaaaat aaaaaagaac gcaaaatcga acgcagacag caaagccgta ctgctcgtaa
    20041 aaataagaaa aattcacata aataaaaaac aagaggttgt gacaaaaggc gtgtcacaat
    20101 ctcttgtttt taagctagac tttttttcat tccgatacaa agtacttcat cttcaataaa
    20161 aggttctgtg caagtatgat acccttgttt ttcataaaaa tctacagccg tcatttcagc
    20221 tactacataa ctagtctgac aaccttctga aaccgcccgt ttttcagcag cttcaagcaa
    20281 ctgaccgcct aacccttttc ctcggtattc ttttgctacg caaaatcgtt ccggcttgaa
    20341 gcaagtaggt gttttctttt cataacgcaa agtagctact ggtctttttt gatcaagaaa
    20401 tagataatag gtttgttgcg ggatatcttt tccgtcgaat tctagttctg caggaatccc
    20461 ttgttctaat acaaaaactt gatatctcaa aaagcagcta gctgcgcgta tccattcatc
    20521 cgctccaact atttttttca tcatattcct ccttgtcatg aatagcagag atgtattgat
    20581 tcctttattc ttatcgtacc aaaaatcgtg gagtatgacc aaaaggaaat gaaaataaaa
    20641 attgagatat tgacagtgat caatagaatg cgtatactaa ttaagtacga aaaggatatg
    20701 atttgttttt taagactcac agaaaattct ttcttggctg aaaaaagaat agtcactaat
    20761 caaattgctc cctttttcta ttttggtaga aataccgacg cagctcagcg ttatcgagtt
    20821 taagaggtaa tgattacaca tcattgcaat caaggtggta ccgcgagttt tcgtccttgg
    20881 ctgtgatggt gtttttttgt atttaattta tagaaagcag gtaatgacat gaaacaatta
    20941 agtagtgcac aagttcgaca aatgtttctt gatttcttcc aatctaaagg acatacgatt
    21001 gaacctagtg cgtctttgat ccccgtgaac gatccaacct tattgtggat caactctggt
    21061 gttgcaacat tgaaaaaata ttttgatggt tcagttgtgc cagaaaatcc aagaatcaca
    21121 aatgcccaaa aatctattcg aacaaatgat atcgaaaatg tcggtaaaac agcacggcac
    21181 catacgatgt ttgagatgct gggtaatttt tcgattggtg actatttcaa aaatgaagcc
    21241 atccactggg catgggaatt tttgactgat ccaaaatgga tggcattcga tccagaaaaa
    21301 ctttatgtaa cagtttatcc aaaagataca gaagcaaaac gcatctggca tgaagaagtt
    21361 ggcctgccac tagatcacat tattgatgta gaagacaact tctgggatat cggtgcaggc
    21421 ccatgtggtc cggatacgga aattttctat gaccgtggag aagcttataa cgatttagca
    21481 gaagacgatc ctgaaaacta tccaggagga gaaaatgaac gttatttaga aatctggaac
    21541 ttagtatttt cagaattcaa ccataagcct gataacacat ttgaaccact tcctcataaa
    21601 aacattgata cggggatggg attggaacgc atggtctcaa tcgttcaaga tgcgtcaacg
    21661 aactttgaga cagacttgtt tatgcctatt atccacgccg tagaaaagct aagcaaacaa
    21721 gtaaaatatg gtgaaaaccc agtaacagat atttcttaca aagtaatcgc agatcatatt
    21781 cgcgcccttt catttgctat tggcgacggt gcattgccat cgaatgaagg gagagggtat
    21841 gtcttacgcc gtttgcttcg tcgtgctgtc atgcatggga aaaaattagg tattgaacaa
    21901 gcattcttat atcgtttagt ccctgttgtt ggggaaatca tggtaagtta ttatcctgaa
    21961 gtacttcaac aaaaagaatt tattgaaaaa gttatccgta cagaagaaga acgtttcaat
    22021 gaaacaatca atgaaggttt ggaaatctta aatcaagtga tccaagaagt aaaaaacgaa
    22081 aacgggacga ctctagcagg aaaagacatc ttcaaactat acgacacata tggcttcccg
    22141 gtagaattaa cggaagaggt ggcagaagat gaaggactga aagttgatca tgctggtttt
    22201 gaaaaagaaa tggatgcaca acgcgaacgt gctcgctctg cccgcagtac tgaaaaatca
    22261 atgggggtac aatctgcttt attgacagat atcaaagtag aaagtaaatt cattggatac
    22321 acagaacaag aatcagatag tcaattattg gtcatcgtta aaggtgagtc tcttttgaat
    22381 gaagtcgcag aaggtgaagc tcaaatgatc tttgatcaaa cacctttcta tgcagaaatg
    22441 ggtgggcaag tagcggatca aggatggatc atgaatgaag taggcgaaat cgtagcagtt
    22501 gttacagatg ttcaaaaagc gcctaatggt caatttatgc acaccgtaga agtaaaagca
    22561 ccaatgagtg aaggacaaac ctatcatcta ttgatcgatc aatcgatgcg caatcggatc
    22621 atcaaaaacc atacagcaac ccatctactg cacaaggcat tgaaagaagt acttggcgag
    22681 catgcaaatc aagctggttc tttagtcgct cctggacatt tacgattcga ctttacccac
    22741 tttggtcaaa tcacttcaga tgaattagct gaaatggaaa gaatcgtcaa cgaaaaaatc
    22801 tgggaagcta tcccagttga aacaattgaa acagatatcg atacagccaa aaacatgggt
    22861 gcaatggctt tgtttggtga aaaatacgga catgacgttc gagtagtaaa tattggcggc
    22921 tggtcaatcg aactgtgcgg aggcacacat gtagcaaata cagaaaacat cggaatcttc
    22981 aaaatcgttt ctgaatcagg aattggtgca ggcgtgcgac gaatcgaggc tgtaacaagt
    23041 aaagaagcgt acgaactact tgaagaagaa gaaaaacaac taaaacaagt agcaacaatt
    23101 gttaaatcac ctcaattgaa agaagttgtt tcaaaagcgg aacaattgca acagcaagta
    23161 agagagctac aaaaggaaaa tgaacaatta gctagcaagt tagcgaatca acaagctggt
    23221 gatgtattca aagatccaga aacgatcaat ggaaaaacaa tcattgcagc acaagtaaaa
    23281 gtgaaagata tgaatcagct gcgtcaatta gctgaccaat ggaaacaaaa aaatatttca
    23341 gatattcttg ttttagcaaa cgaacaagaa ggaaaagtca gtctgctagc ggcaatgacg
    23401 aaagaatcaa atgaagctgg actaaaagct ggcgacttga ttaaagcaat cgcaccgaaa
    23461 gtaggcggtg gcggtggtgg acgtccagac atggcacaag ctggcgggaa aaatcctgct
    23521 ggtattccag atgcacttaa agctgcgaaa gaatgggttg cgcaacaata gggcgtttac
    23581 gtttgacaga aaaacaaaag ttcaaacatt gaacgaaaca tgattatggg ggcgaagaca
    23641 aacaagtctt gccctcatac ttttttcaat tggatggcgc atctaatcaa atggtcggat
    23701 tcggatggtc tttagttgac gaaagtgaca gaaattttta cgattagtat aaataaaaag
    23761 gggtgaaaaa atgaatagta gaaataagac ctttcgacta gtcttacgtg cgatcttgtt
    23821 agcaatcatc atcgtacaag cgatggttcc ttggctgggg tttattccat taggattcat
    23881 tagtttaaca attatccata tcacagtaat tattgctgca gtagtattag gcccaaaaga
    23941 tggcatggtg attggcttgt tttggggaat cgctacaatt gttcgtgcct atgccatgcc
    24001 aacaacgcca tttgatacat tagtgttcac gaatccgatt atctcggtag ttcccagagt
    24061 acttgttggc ttagtagcag gcttggtttt ccactggatt tatcaaaaaa ataaatcaat
    24121 aacgatcagc tcagcctttg caggggtact gggttcattg gtcaatacag tcttagtgtt
    24181 aggattcatg ggattatttt atacaggtgc aacggcagat gcctatggtg tagactctgc
    24241 tcttttattt aaaaccttag caggggttgc agcaatcaat gggataccag aagcaatagg
    24301 tgcgggaatc ataacaccat tactagcaaa agcgttattt gcagcaacac cgttgaaacc
    24361 agaataatga ccatctttta atagtaatag gacgcacgat agatagaaaa aggaagagag
    24421 gagaggaaaa caatcgtcct ctccatcttt ttacctgagc aacaagcaaa ctagaagggg
    24481 taaaaaactt ggctaagaaa caagaagaaa aaaagaaaca aataccgcaa cagcggacaa
    24541 aaaaaggagt actagcgggt atcttaggat tggttacgaa tctgatcttg tttgtctcta
    24601 agctaatgat cgggcttttt tcaggaagtg tctccattat ggctgatgca gtgaacagtc
    24661 tatcagatac tgcttcatcc atattgactt tgatcggatt taaaattgct gcaaagccag
    24721 cagatcagga acatccttat ggacacgaac gcttcgaata tatcagtggg ttatttgttt
    24781 cgatcatcat tacttatgta ggatttcaat ttttagagtc ttctgctgaa aaaatcatcc
    24841 atccagaaaa tgtctcttta acacccatcg tcttttttgt cctaatcatt tccatctttt
    24901 tgaaatttgc acaaggcaaa atgtatcagg ttattgcgca atccattgat tctgaaacat
    24961 tgagagctac agcaacagat agttataatg atgtctttac tacactggct gtattgcttt
    25021 cagcagcaat cgaatgggta actggatggc gtattgatgg atatataggt tttgctttag
    25081 cattatatat cctttatagt ggattgacga tgcttcgaaa ttttgtatac gaactattag
    25141 gaagtcgccc aacgccagca gagattgaag aaatggaaga gcatttaaat gcttgcgatg
    25201 atatacttgg ctatcatgat ttattggtgc ataactatgg acccaataaa cgctttgcat
    25261 cagtccacat cgaagtagat gaatcaatga atttgaatgt ggctcataag attattgatc
    25321 tgatagaaaa agactttaaa aagtctttgg atgtggaact tgtctgtcac ttggatccaa
    25381 tgcctgttga taacgaaaaa tactataata tcttgcggca gttaaagcaa attcttcttg
    25441 aaacaggtac agggcttaat attcatgatt ttagagtaat tcatcaagga aaaacgctgc
    25501 aatttgatgt agtggtccct gagaaattcc agtttgttga tacagaacta gaaagaatct
    25561 tacgtagtca aatagaagaa aaaatcggag attaccaatt ggaaattacg tttgatcaca
    25621 attatctttt atattaaatg agaaactata aaaagactcc ttaaagtttt taatttaaag
    25681 agtctttttt tcttttaatt gtttatggag agcctgacgt gcaagattat ctgctccttt
    25741 atttttgttt tcgggcaacc attgcaggat caataaagaa aattcggata gaagttcttg
    25801 gattctttga aggtagggaa ggaagagctc gttcttcgtt gcgtttttgt cgaccgtaga
    25861 aactagtgtt ttgctgtctg aaaaacagaa aatcgtttca ttctgtaagt cgtttttctt
    25921 tagaaattct aaagttttta aaaagacttc gaactctgct tgatggtttg tccctataga
    25981 aagcgggata gatagctgtt cttgtttacc ttgatagaca atcaagattc cacctccact
    26041 aggaccaggg ttgccttttg tagaggcatc aatgtatact tttaacataa aaagagattc
    26101 tcctttttgt tcaaactaat gttatggtaa gattatatca ttactgaatc ggtgagaggt
    26161 tagagaatga aaaaaaatat tcgatggcag cctgaactgg cacatacgat catctattgg
    26221 tcattgacct tgatcattct gttttttagt ctgattcttt ctttagaaaa cacaggtcct
    26281 tattggaaaa gcaattttgt tttaggtatt tttcttgttt ttctatttct aggtttgcgg
    26341 cgttctttta ttttaaaatc aaactctgtg attatacgtt atgctgcttt ttggaaagac
    26401 cgagaaatat ctgtcggaca gattcgatct ttgacgccaa ctaatactgg ggtgcaaatg
    26461 atgttgcaag atgagaaagc cccatctgtt ttcatgatgc gaaaaaaaga tcaagtagtc
    26521 ctttttgatc attttgaaaa aaacggaccc aaatcagaat caattcctta tccttatacg
    26581 aaaaaaagaa aaagctaatt ttaaaaatca gaacctaaaa aaacgaattt ttacaggaaa
    26641 agaaaagagt gagacgtacg gttcatcccg tttagcgtct cactctattt gtttatctat
    26701 tcatgcatac tagtatcggt agtctgaacg ttgcctttct tgacatgggc agcttgtgtt
    26761 cctcgattcc cttcgatgat ctcaaattcg accacttgat tttcttccaa agttttgaat
    26821 ccctcttctt caatcgcagt aaaatgtaca aaaatttctt ctgtgtcatc ataactgata
    26881 aagccgtatc cttttttgtt gtcaaaccat tttacaattc ctgtggtcaa agcactcacg
    26941 acctttctaa agtctgatca tagccttcat tataaagagt atttataagt acgtcaaata
    27001 aattaaaaac ctgtaatttt atgttttttt tctgaataaa aaaaggtata attggcatat
    27061 cttaacaaag aagatatttt gttgaataaa gttcgtgaaa aggggtaatc aaatgaagaa
    27121 tgatctgcaa atttcacaag aaacagagct agaaccgatt gttgaagtgg ctagtaaatt
    27181 agatcttaag gaagacgatt tggaactgta tgggaaatat aaggcgaaaa tcaatttttc
    27241 tggaatcaat cgatcaaagg ataacgctga ggggcatttg atcttagtta cttccatcaa
    27301 tccaacacca gctggcgaag gaaagtcaac gatcactgta gggttaggag acgcgctcaa
    27361 taaaatcaat aaaaaatctg ttattgcatt gcgagaaccc tcacttggtc ctgtgatggg
    27421 aatcaaaggt ggtgctgcag gcggaggata tgcacaggta ttgcctatgg aagatatcaa
    27481 cttgcatttt actggtgaca tgcatgctat cacaacagct aataatgcat tatctgctct
    27541 tttagataat catattcatc aaggaaatga attgaacatc gatgcccgcc gagtgatttg
    27601 gaaacgggta gtcgatctca atgatcgtga actgcgtaaa gtaattgttg gcttgggtgg
    27661 gccaatccaa ggtgttccaa gagaagatgg gtttgatatc acagttgcca gcgaaatcat
    27721 ggctatcttg tgtcttgcgg cagaccttga agatttaaag gctcgtcttg caaggatcgt
    27781 tgtcggctat acatttgacc gtcagcctgt gactgctgga gacttgaaag cagaaggcgc
    27841 tctggcatta ctgctgaaag atgcaatcaa gccgaatctt gtacaaacaa tttacggaac
    27901 accagctttt gtccacgggg gaccgtttgc caatatcgct catggctgca atagtatttt
    27961 agctaccaag acagcattga gattagcaga ttacgttgtc acagaggcag gatttggtgc
    28021 agatcttggc ggagagaaat ttttagatat caaagtacct aacttgaaaa aagcgccgga
    28081 tgctgtggtg attgtcgcca ctgtccgtgc attgaaaatg catggcggaa tgaaaaaaga
    28141 tgaacttaaa aatgagaatc ttgatgcttt gaaagtaggt tttgcaaatt taaagcgcca
    28201 tatacgtaat atggaacaat atcagctacc tgtgatcgtc gcaatcaatg aattcgtaac
    28261 agatacagac aatgaactca cactgttgga gcacttgtgt gaaaatcagg ggattcttgc
    28321 caagagagcg agtgtatggg cagatggagc agaaggcggc gttgatttag caaaagctgt
    28381 agtacgattg attgatagaa aagaagctaa ttacaagcca ttataccgcc tagaagaaac
    28441 gatacaagaa aagacagaaa caatcgtcaa gaaaatttac ggaggaaatg gtgttgtttt
    28501 cagcaaaaaa gcgcaaaaac aaatagaaga gtttacgaag aatggatggg acaaactgcc
    28561 gatttgtatg gcaaaaacac agtattcatt ttctgatgat ccttcactgc taggtgcacc
    28621 ggaagatttc accatcacga tccgcgaaat catcccaaaa ttaggggcag gatttttagt
    28681 agccttgact ggagatgtta tgacgatgcc tggtttgcct aaaaaacctg ctgcattgaa
    28741 tatggacgtg acaaatgacg gagaagtgtt gggattgttt taagcttgat tataatctac
    28801 catcgaagga gctgtatttt gactacagct tcttttttta gacgataggg aattaatttt
    28861 gtttacacta ggatcattct ttttctttat tgtattttga gctatactaa agcataatag
    28921 gagggtatga tgaaaacgac atttataaga aaaaccggtt tttacggtat gggtagctcg
    28981 attcagctaa gaatcaacaa tgaaaaacgc agtgttctca gtcatgaaca aaagataacg
    29041 cttgatctgg aagtgccctt tacgatgcag gtcacctttt attggctaaa aagccccgtt
    29101 tatacagtga aagaagcaaa gcctgcttat gtgatcacaa tgaattactt gattcttcaa
    29161 atctatccgt ttttatttct agcaactggg ttgggggccg ttttcctaca aaatttattt
    29221 ttcagttttt ttgcagttat tgtgttgatt gcgttctttt tctatataaa aaaccgggca
    29281 tatttgatca aggaggaaaa ttatggcaaa ctctgatctt tttttaatca gtttcaatcg
    29341 aatcgaaaaa tggatgagag acgagatggg aaatgcacga aatatgggat ttaccgaact
    29401 tgtcagacgc ctagctcaaa gaaaacaatt aatgatcaga aaatacgaag atgatctttt
    29461 acagcttgct cagttaagga atgcgatcgt ccatgatcgg atcgcagtcg attttgtcat
    29521 tgcagagcca aatgattgga cagtcaagcg aatccagctg atcgaacaag aactgattca
    29581 gcctgaaacc gtgctgcctc gatttgcaaa acgtgtaaca ggttttgaac aagatcttcc
    29641 gttacttgaa ctgttaaaga tagtagcaga aaaaaggtat tcacaatttc cgttatacaa
    29701 taaaggaaaa tttgtagcat tgattacttt acgtaatatc gggttttggc tagcaaaaga
    29761 aagccaaaaa ggtccggttg atttaacaaa taaacgagcg ttggatttga tcatccaaaa
    29821 cggaaagtac acaaattatc attttgttcc agctaaaaca catatttacg aggttgaagc
    29881 gatgtttcgt gagcaaggga ctttggaagc cgtacttatc actaaagatg ggaatcccga
    29941 tggtaatctg ctcgggatcg tacgacctcg tgatatttat aaacaaatag aaaaggattg
    30001 attttttgtt aattatttat tggatattca gcgctttgct agtaggagtg gatcagttgg
    30061 tcaaatggtg gatcactgat aatctagcgc ttggagagac gaaaaatctt ataccaggta
    30121 ttttgtcact gaatcatatt cgaaacactg gagccgcatg gagtatgcta gaagggaaga
    30181 tgtggttctt tactatagtg acactcattg cagtcgttgt gattctcaca ttgatgatta
    30241 aaaatcgtga aaaaggtaat cgctggttca tgattggatt gagtttgatt ctagctggtg
    30301 cgattggcaa ttttatcgac cgtattcgtc ttggctatgt agtagatatg ttccagacag
    30361 attttatgag ctttccgatt tttaacgtag cagatgtgac acttgtatgt ggagtgattt
    30421 gtatgttaat ttatattatt ttagatgaaa aggatcaaag aaaaaaatga cagaacttaa
    30481 aacgacgatt caccatgaat ctggtaggat cgataaagta ttgactaatt tattcaccga
    30541 ttacagtcgt tcgcaaattc agctgtggct gaaaaatgga gcggtttctg tcaatggaca
    30601 gccagtcaaa gcaaattaca aagtaaaaca aggagacgag attactgtct tgatccccga
    30661 accagaaaca ttagatgtgg tcccagaaaa tatcccgttg acgatcgttt acgaagatga
    30721 agatgtagcg gtgataaaca aacctcaagg aatggtcgtt catccttcag caggtcatac
    30781 gcatggcaca atggtcaatg cgctgatgta ccatatcaag gatctttcta cgatcaacgg
    30841 cgttattcgt ccaggtattg ttcaccgaat cgacaaagat acttccggac tgctaatggt
    30901 tgccaaaaat gaccaggcac acgaatcatt agctcagcaa ttaaaagata agacctcttt
    30961 gcgcaaatat gttgctttag tccatggaga gattcctcac gaaaaaggaa ccatcaatgc
    31021 gccaatcggc cgttcgaagg aaaaccggaa aatgcaggcg ataagagaag atgggaagtc
    31081 ggctgtcaca cattttactg tattggaacg tttcgaagga tttactctgg ttgaattaaa
    31141 actagaaacc ggaagaacac accagatccg tgtgcatatg aagtatatcg gttatccgtt
    31201 agctggagat ccagtatacg gaccgaagaa aactttgaaa ggcaatggtc agtttctcca
    31261 tgcgcaaatg ttaggtttta cgcacccgac tacaaacgaa aagatgatct ttgaagcacc
    31321 tcttccagaa gtttttgaaa aaactttaga aaaattaaga aaaaacattg cattttaatt
    31381 tagataacgg tatagtaatt aacaacgaaa gcataaaact taatagaatt cctttaagtt
    31441 tagtcccgtg aggctaagaa gggtgggagc aaggatacgt atggtgcgcc ataagtagat
    31501 tgctcattga catagtatag ctatcagtgt atcagtatac gctcagtcta aacattcctc
    31561 ctgtcctctt agggcaggag tttttttatg caaaaaagta gggggaatga aaatgcaagc
    31621 aaaagaagta gtagatcagg taaccatgaa gcgagcattg actcgaatta cttatgagat
    31681 tatcgagaga aaccatagta tccaagatat cgtattggta ggtatcaaaa cacgaggaat
    31741 ctacattgca tcaagaattg ccgaacgcct gaagcagcta gaagatatcg atattcctgt
    31801 aggagaactg gatatcactt tataccgaga tgacaaaaaa gaaaatccag aagaaccgga
    31861 acttcattct tctgacatcc cagtttcctt agaaggaaaa gaagttattt tgattgatga
    31921 tgttttgtat acaggacgaa cgattcgagc agcaatggac gcagtaatgg attttggcag
    31981 accaagaaaa atttcattag cagtcctggt cgatcgtggg catcgagaat tgccaatccg
    32041 tgcagattat gttgggaaga acattccaac atctcgggcg gaagagattt tagtagagat
    32101 gcaagagctt gatggacaag atcgcatcat gattttgaaa gaggaagatt agaggagaga
    32161 gaaacatgtc ggagaaaaaa ggatttcata atgatgacgt ggtgttagat atccacgatc
    32221 gcccaacccc aggacattgg gtaggattaa gtttacaaca cttgttcacg atgttcggag
    32281 caaccgtgtt agtccccatc ttagtaggta tcgatccagg tattgcgcta gtcagttcag
    32341 gactaggtac attggtgtac ttatttacta ctaaaggaaa gatacctgcg tacttaggaa
    32401 gcagcttcgc tttcattgca gcgatgcagc ttttgatgaa aacagatggt tatccagcga
    32461 ttgcacaagg agcagtcaca actggattag tctatttgat tgttgcgctg atcatcaaaa
    32521 agatcggttc agcatggctg gataaaatct tgcctccaat cgtcgtcggt ccagtcatca
    32581 tggttatcgg gttgggactt gcggcaaatg cagcaacaaa tgctatgtat aatcagggca
    32641 cgtatgattt caagtatatt gctgtagcgc tgatcacatt aggattaaca attttcttca
    32701 acatgtggct aagaggtttt ctcggattga ttccgatttt actaggtatt attagcggat
    32761 acatcgtagc cttgctgttt ggaatcgtgg atactaagcc aatcgtagac gctgcctggt
    32821 ttgctgttcc taactttgag attccctttg tacaatatga accaaagcta tatcttggag
    32881 caatcacaac aatggcaccc atcgcttttg tgacgatgac agagcatatc ggccatctta
    32941 tggtattgaa taaactaact aaacgtaatt tctttgaaga accgggtctt cataagacgt
    33001 tgacaggtga tggattagca caaatcgtgg cagcctttgt cggaggtcct cctgtaacaa
    33061 gttacggtga aaacattggt gttctggcta tcacacgtgt ccatagcgtg tttgtgatcg
    33121 gtggagctgc agtatttgca gtcattttag gattcgtagg aaaattaagc gctttgattt
    33181 taagtatccc tggaccagtc atctcaggaa tcagttttat cttatttgga gtaattgccg
    33241 caagtggaat gaagatttta atcgagaaca aaatcgattt tgataaaaag aaaaatctgt
    33301 tgattgcttc agttatctta gtagtaggaa ttggcggact cgtctttgaa gtaggcacat
    33361 ttacattgtc ggcaatggca ttagcaactg tacttggcat cattttaaat cttatcttac
    33421 cagaaacttc acgaagcgaa gaaaactaag gaggaaacat tatgatcatc aaatcagaac
    33481 gtatcagctt aaagcattta ttgaccgttg aggcacttac tgatcaagaa gttatgggct
    33541 tgatccgcag agggcaggaa ttcaagcaag gtgctaattg gactccgcaa aagcgccagt
    33601 actttgctac aaacctcttt ttcgaaaata gtactcgcac acataaaagc ttcgatgtag
    33661 cggagaaaaa actagggcta gaagtgattg aatttgaagc aagcacaagc tctgtccaaa
    33721 aaggggaaac actatacgat acagtactga cgatgtcagc attaggcgta gacgtagcgg
    33781 ttatccgtca tggtgatgag aactattatg acgaactgat acaaagtaaa acaatccagt
    33841 gttcgattat caatggagga gacggaagcg gacagcatcc aacccaatgc ttgcttgatt
    33901 tgatgaccat ttatgaagaa tttggtcatt ttgaaggtct gaaagttgcc atcgtaggtg
    33961 atatcactca ctctcgcgta gctaaatcca atatgcagat gttaaaacgt ctaggcgcac
    34021 aagtattctt ctcaggtcct agagaatggt acgacgatga attcgaagtg tacggacatt
    34081 atatgccatt ggatgaacta ctagatcaag ttgatgtcat gatgatgctt cgggtgcaac
    34141 acgaacgcca tgatggaaaa gaaagtttct caaaagaagg ctaccatcag gaacatgggt
    34201 taacagttga gcgtgcgaag aagttgcaaa aacatgcaat cattatgcac ccagcaccag
    34261 ttaaccgtga tgtagaatta gctgattcat tagtggaagg attacagtcg cggatcgttc
    34321 agcaaatgag caatggagta ttcgtcagaa tggcaatctt agaagcagtg ctaaatggaa
    34381 aagcgtaatt ataggaggat gaaaaaatga aaacactcat taaaaacggt cagatcaata
    34441 cacgaaaaaa tatgacgaca ccagcagaaa tttggataga agatgggcga atcaaagcaa
    34501 tcggtacagg attctcagaa gccgaatttg atgaagtgtt tgatgcaaag ggacaattga
    34561 tcacacctgg tttagtggac gtccatgttc atttgagaga gccaggattt acatacaagg
    34621 agacgatcga agctgggact cgctcagctg ctcgtggcgg tttcactact gtatgcgcga
    34681 tgccgaattt aaatcctgta cctgatacgg cagaaaagct gagacaagta tatgacatca
    34741 ttagaaaaga tgcagttgtg aaggtattgc agtatgcgcc aatcacagaa aatctgcgaa
    34801 gtgagaaatt agttgatcaa gaagcactga ttgaagaagg tgcttttgct ttcacaaatg
    34861 acggtgtagg tgtccaaaca gctgggacga tgtatctggc tatgaaagaa gctgcaaaaa
    34921 acaataaagc cctagtagcc catacagaag atgaatcttt attatttggc ggcgttatgc
    34981 acgctggcaa gaaggcagaa gaacttggtc ttcccgggat cttaagcgta accgagtctt
    35041 ctcaaattgc tagagatctg ttgctagctg aagcaactgg tgttcattac catgtttgcc
    35101 atgtttctac taaagaaagt gtccgtgtaa tcagagacgc aaaaaaagca ggaatccacg
    35161 taacagcaga agtttcaccc catcatctta ttttgatcga cgaagatatt cccgaagatt
    35221 tcggcttttg gaaaatgaac ccgccgctaa gaggacggga ggacagagaa gctttgatcg
    35281 aaggcttgct tgacggtacg atcgattgca tcgctacaga ccatgccccc catggattag
    35341 aagaaaaaag ccaaagtttc atgaaatcac catttggtat cgtaggaagc gaaacagcgt
    35401 ttcaactgat ctatactcat tttgtagaaa ctggtcgatt cacgttggaa caagtgatca
    35461 attggttggc agttaaaccg gctgagattt ttggtttgaa tgcaggaacg ctaacggttg
    35521 gtgcccccgc tgatgtagca gtttttgata ttacacaaac ttgcacgatc gataaagaag
    35581 actttttatc aaaaggggag aatacacctt ttatcggctg gaaagtaaaa ggcgaaacac
    35641 aaatgacttt tgtcaacgga aaacttgtgt ggcagaaggg agaataaaag tatggaacgt
    35701 ttgcttatat tagaagatgg aacagttttc gaaggaaagg cttttggtgc agatgctagt
    35761 gtcttcggag aaatcgtttt tacaacaagt atgactggtt atcaagaaac tatcactgat
    35821 caaagtttta acggtcaaat cattacattt acctatccta tggtgggtaa ttatggggta
    35881 aatagagatg attatgaatc gatttctcca acatgcaaag gagtagtagt aaaagaacat
    35941 gcacgtgtag cttcaaactg gcgaaaccaa atgacgctag attcattttt gaaacaaaaa
    36001 ggaattcctg gaatatcagg tatcgatacg cgcgccctta ctcgcaaaat ccgtgaagtc
    36061 ggaacgatga aagctagtat cgttgatgct ggagattcct tcgaacatgc atttgatcag
    36121 ctgaaagcaa ccgttctagc aaccaatcaa gtgcagcaag tctcaactaa caaaccttat
    36181 ccaagtccag gaacaggtcg gaaagtcatt gtcattgact tcggcttgaa acatagtatt
    36241 ttacgagaat tgagtaaaag aaactgtaac ctcacagtac ttccctataa cacagatgcc
    36301 gagacgattt tagcgctatc accagacgga gtcatgctga cgaatggacc tggtgatcca
    36361 aaagatgtgc cagaagcaat cgaaatgatc aaaaaaatcc aaggaaaagt accgattttt
    36421 ggtatttgtc tcggtcacca gttatttgct ttagccaacg gtgcagatac ctataaaatg
    36481 aaattcggac atcgaggatt aaatcacccg gtacgagaag tagcgaccgg aagaattgat
    36541 tttacttctc aaaatcatgg ctatgcagtc gatgaagcca cgattgatcc agaaaaactc
    36601 atcgtaactc atgtagaagt aaatgacgga acagtagaag gagtcaaaca cagagattat
    36661 cccgctttca gtgtgcagtt tcatccagat gctgcccctg gtccgcatga tgcactacat
    36721 ttatttgatg aatttatgga aatgatggac gcaggaaagg agcagtaaac agatgccgaa
    36781 acgtacagat atcaagaaaa ttatggtgat tggatctggt ccgatcatca ttggtcaagc
    36841 cgcagaattt gactacgcag gaacacaagc ctgccttgcc ttaaaagaag aaggatatga
    36901 agtggtttta gtaaattcta atcctgcgac gatcatgacg gataaagaag tggcagatca
    36961 agtatatatc gaaccgatca cacttgaatt tgtatcaagg attcttcgaa aagagcgtcc
    37021 agatgctctc cttccaactt taggcggaca gacaggcctc aatatggcaa tggagctggc
    37081 agcatcaggt attttagaag aattgaacat agaacttcta ggaacaaagc tttctgcgat
    37141 cgatcaagca gaagatcgtg atctattcaa acagttgatg aaagaattga atcagccaat
    37201 ccccgagtct gaaatcgtca atacagtaga agaagctgta gattttgcaa ataaaatcgg
    37261 ctatcctgtc atcgttcgtc cagcctttac tttaggcgga actggtggag gtatgtgtga
    37321 aaatgaagaa gaattacgta tcgtatcaga aaatggatta agtctttccc ccgctaccca
    37381 atgtttgatt gaacgaagta ttgcaggttt taaagaaatt gaatatgaag tgatgagaga
    37441 ttcggctgat aatgcaatcg ttgtctgcaa tatggaaaat tttgacccgg tgggtatcca
    37501 tacaggtgat tctatcgtat ttgcccctag tcaaacattg tctgaccatg aatatcaaat
    37561 gcttcgtgat gcttctcttt cgattattcg agcacttaag atcgagggag gctgtaatgt
    37621 ccaactcgct cttgatccac atagttttaa ttattatgtg attgaagtca atccgcgtgt
    37681 ttctcgttca tctgcgttag caagtaaggc gactggctat ccgatcgcta agctagctgc
    37741 taaaatagcg cttggattaa cattagacga aatgaaaaat cctgttacag gaacgacttt
    37801 tgcagaattt gaacctgcat tagattatgt tgtagctaaa attcctcgct ggccgtttga
    37861 taagtttgaa aacggagaac ggcttttagg cacgcagatg aaagcaactg gtgaagtgat
    37921 ggctatcggt cggaatatcg aagagtcttt attgaaagct gtgcgttctt tagaaatcgg
    37981 tacacaccat atcgaacttc ctgagttggc agacatcacg gacgatgacc taatccaaaa
    38041 agtggttcga gcacaagatg atcgcctgtt ttatgtagct gaagctatta gaagagggta
    38101 caccatagaa gaattagcgg atttgacaaa aatcgattta ttctttttag ataaactgtt
    38161 gcatatcgta gaaatcgaac agcagttgaa acagcagccg ttggatgaca agatattgaa
    38221 aacagcgaaa caaaacggtt ttacagacaa aaaagtggcg gagctgtggg aaataaacga
    38281 agaagccgtt cgccagatga gaaaagaaaa tgatttgatt cctgtatata agatggttga
    38341 cacttgtgca gcggaattcg aatcaaaaac accttatttt tatagttcct atgaatatga
    38401 aaatgaaagt tcacgatcag aaaagccctc aattctagta ctgggatctg gtcctatccg
    38461 tatcggacaa ggggttgaat ttgactatgc gactgtgcat tctgtcaaag caatccagca
    38521 ggcaggatac gaagcaatca tcatgaacag caatcctgaa acagtttcga ctgacttttc
    38581 tatttcggat aaattatatt ttgagccatt gaccttcgaa gatgtgatga atgtgatcga
    38641 gctagaacag ccgataggag taatcgttca atttggtggt cagacagcaa tcaacttagc
    38701 cgaacctttg actgctgctg gtgtaaaaat cttagggaca acgatcgaag acttggatcg
    38761 tgcggaaaat cgtgatttat tcgagcaagc gctgaaagag ttggatattc ctcaaccttt
    38821 aggggatact gctacaagta aagaagaagc cgtggttatc gcttcgagaa ttggttatcc
    38881 agttcttgtt cgtccaagct atgtattagg cggacgtgcg atggaaatcg ttgaaaacca
    38941 gcgagattta gaagattaca tggaacatgc tgtgaaagca tctccagaac atccagtact
    39001 agtggaccgt tacttgcttg gtggtgaatg cgaagtggat gcgatttgtg acggtgaaac
    39061 agtactcatc ccagggatca tggagcatat tgagcgtgct ggtgtccatt caggggattc
    39121 aatggctgtg tatccacctc aaacattatc tgaatcgatc aagaaaacga tcgaagatta
    39181 cacgatccgc ttagcattag gtttgaattg tgtcgggatg atgaatatcc agtttgtcat
    39241 ccatgaaaat gaggtttatg tgatcgaagt caatcctcga gcaagtcgga ctgttccatt
    39301 cttaagtaag gtgacaggaa ttccaatggc acaaattgcg acgaaagcta ttttgggaga
    39361 gaaactaaag acacttggtt atgaaaacgg actttatcca gaaacaaaag aagttcatgt
    39421 taaagcacct gtcttttctt ttacgaaatt gcagaaagtt gatacttact tagggcctga
    39481 aatgaaatcg actggagaag taatgggatc tgacagacaa ttagacaaag ctttgtacaa
    39541 ggcattcgaa gcttctggat tacacttgcc tgaatatgga gccgtattat ttacgattgc
    39601 ggacgaaaca aaagaagaag cactagaatt agctaaacgt tttacagaaa ttggctatag
    39661 tctgatagga acgaaacata cagctgctta tttcgaaaaa aatggtctgg tcgttgaaac
    39721 agtagcgaaa atttctgagg aagcagcaga aaaaaatgta gtcgacttaa taagagatgg
    39781 taaaacacaa gtggttgtca atacgattga taaagatcgg ggaaatgctt ctaaagacgg
    39841 tttcatcatc cgaagagaag cagttgaaca tggtattcca ttgtttactt ctttagacac
    39901 agcagatgcg atcgttcgag tgatggaatc ccgtgctttt tcaactaacc ctatttaacg
    39961 gcgagcacat gttaagacaa aaagacgaaa aaccgttgca ggaggggaca gatgaaacaa
    40021 gaaatgatgg aaatcgttca gcaaaaacag ctggcaccaa agatttttga aatgacattg
    40081 aaaggtgatc tagtaaaaga aatgcagcaa ccaggacagt tcttgcatat tctcgtgccg
    40141 cgtgcagact tgctattacg ccgaccgatt agcttgaatc atatcgacca cgaaacaaat
    40201 acttgccgta tcatttatcg ggtggaaggt gaggggacta gagtcttctc tactctttct
    40261 gccggagacc atttagatgt catgggtcct ttaggcaatg gttttgattt gtccaatctt
    40321 catacaggag acgaagtatt tataatcggt ggaggaatcg gtgtccctcc tctatatgag
    40381 ttatcaaaac agttagtcga aaaaggaatt aagccaacgc attttttagg ttttgccaca
    40441 catgaagtcg tttattatga aaaagaattt aatcgtctag gagaaacaag aatcgctact
    40501 gacgatggaa catacggtat gcacggaaat gtcggtaatt tattgttagg aacgaagacg
    40561 gaacctacag ctgtttttgc atgtggttca aacgggttat tgaagacagt ggaacagctg
    40621 ttttcttcgc atcaaaatgt ccagttatca ttagaatccc gcatggcatg tgggataggg
    40681 gcttgctatg cttgcgtttg tcatacgccg gaagatgaaa caggaacaaa aagtgtgaaa
    40741 gtttgtgacg aaggaccgat ttttaaaata ggagaggtaa ttatatgaat gagaatcctt
    40801 tagcggtcaa acttcctggc ctagaattaa aaaatcctat catgccggca agcggctgtt
    40861 tcggttttgg caaagaatac ggaaaatact atgatttgaa tcagcttgga tcgatcatgg
    40921 tcaaggcaac gactccaaat gcccgctttg gcaatcccac gccaagagtg gcggaaacac
    40981 catctggcat gttgaatgca attggtttgc aaaatcctgg tctagacgtt gttatgcata
    41041 aacttcttcc agaattagag gagtacaatg agttacccat catcgcaaat gttgctggtg
    41101 cttgtgaaga agactatgta gaagtttgta gcaaaattgg cgaagcacca aatgtgaaag
    41161 caatcgagct gaacatttct tgcccaaatg tcaaacatgg cggaattgct tttggaacag
    41221 attctgatgt tgcttttcaa ttaacacaag cagtaaagaa agtttcttct gttcctgtct
    41281 atgtcaaatt gtccccaaat gtaacagaca tcgttccaat cgctcaggca atcgaagctg
    41341 gaggggcaga tgggtttacg atgatcaata ctcttcttgg tatgcggatt gaccttaaga
    41401 caagaaaacc gattttagcc aatcaaacag gtggactctc cggaccagca atcaagcctg
    41461 tcgctattcg tttgattcac caagtagcaa gtatatccag ccttccaatc attgggatgg
    41521 gcggcgtgca gacagtggat gatgttcttg agatgtttat ggctggagca agtgctgtag
    41581 ctatagggac cgcgaatttc accgatccgt atatctgtcc taaactaatt aaagagctcc
    41641 ctgtgcgaat gagcgaacta ggcatcgaat cgctggaaag attaatcaaa gaagtgagag
    41701 aggaacgtga acgatgaatc aactagacca acgaccaatt attgcattag atttttcaac
    41761 taggcaagag gtagaagact ttcttcgatt tttcccgaaa gaagaaaaat tgtttgtgaa
    41821 aatcggaatg gaattgttct accaagaagg acctgaaatc gtacgttatc taaaagaggc
    41881 tggtcataat gtatttttag atctgaaact tcacgatatt ccgaatacgg tcgaaaaagc
    41941 aatgcgggga ttggcaaagc ttggtgttga tctcacctgt gttcatgccg ctggcggtat
    42001 tcgtatgatg gaggcggcaa tgcgaggatt agaagagggg actccagaag gtggaaaacg
    42061 tccgctactt ctagctatca ctcagcttac ttctacaagt gaagaagaaa tgcatgccga
    42121 tcaactaatc gaagtttctt tagaaaaaag tgtgatccat tatgctagct gtgccaaaaa
    42181 agcaggatta gatggtgtgg tttcttctgc ttgggaagta gaagcgatca aagaaatggc
    42241 aggagacgaa tttgtttgct taactccggg tataagacca gaaggaacag tcgcaggaga
    42301 tcagacaaga gtagtcacac cttctcaggc aaaaaagatc ggctcaactt ttatcgttat
    42361 aggtcgacca atcactcagt caactaatcc ttataaagca tatcaaacga ttcaaacaga
    42421 atggagctaa cctaaaatga atgttgaaca aagtatcgca aaagacttat tagaaattga
    42481 agcagtattt ttaaatccaa gtaatccttt tacatgggca agtggaatca aaagcccaat
    42541 ttactgtgat aaccgaatca cgatgagcta tccaaaagtc cgaaaagaga tcgcaaaagg
    42601 attagcaagc aaaatcaaag aagcttaccc tgaagtacaa gtgattgctg gaactgcaac
    42661 agcaggaatc cctcatgccg catgggtagc ggaaatactg gatttgccaa tggtctatat
    42721 tcgaagtaag gcgaaagatc atgggaaagg taaccaaatc gaaggacgta tatttgaagg
    42781 tcagaagatg gttgtgatcg aggatttgat ttctacaggc ggaagtgtct tggaagcagc
    42841 tgaagcagca aaaagagaag gtgcggatat ccttggtgtt gcggctatct ttacgtatga
    42901 gttgccaaaa ggaaaagcaa atttcgaaaa agccgaaatc cctcttatga cgttgaccaa
    42961 ttattctgta ttgattgaag cagctttaga agatcgttat atcgatgagc aagaattaac
    43021 tctgctaaaa gaatggaaaa aagatcctga aaattggcag atttaagcgc gcttgatgaa
    43081 gatagagaaa tccgatttgt gtctccagaa tcatcaattg gaatagcaaa aatccaaaaa
    43141 ctgattgcga gaagcatctt ttcatcaggt atgaaaagaa acttctatcg tgatcagttt
    43201 ttgatttttt taaaattcat ttttctaaga ctggttagca gaaagaagag aataatctct
    43261 tctctttttt ctattatata tagattttct ctattttttg gtaagggagc tgtttcaaaa
    43321 atggtaaaga gcggaaatgt tgcgaatatc agttatttaa tgatatgata ttcggaaaaa
    43381 gaaatctcaa agggaggtaa cttttatggg gttgaatgaa tttattttag gattgaacaa
    43441 tttagagacg atcacaaggg cacctggatt ttttaaatat acagagcata cagttgcagc
    43501 acattcgtat cgtgtagcat ctattgcgca agttctagga gacattgaag aagcgaatgg
    43561 acaaccgatc gattggaaac tgctctatga aaaatcattg aatcatgact acacagagcg
    43621 ttttattggt gatatcaaaa cgccggtgaa atacgcaagc aaagagttaa gagggatgct
    43681 gcaaacagtt gaagaaaaga tgacggacga attcatcaac caagagattc cagctgagtt
    43741 ccaagaagtt tatcgccgtc ggttgttcga aggaaaagat gaaaccgtag aaggagaact
    43801 attagccatt tcagataagg ttgaccttct ttatgaatct tttgatgaaa tcatcaaaaa
    43861 taacccggaa aaagtttata aggagatgtt cattgaggca gtgactacga ttaaaagctt
    43921 tgaacatcgg ccttctgttc agtatttctt taaagagata tttccggaac tattgaacca
    43981 ggaattttat ggaaaagagg cgtttttaga aaccgtctca acgattgtaa agaaagaaga
    44041 aaaataaaaa tgatatgaga tgcaggggga agtatagttg aaaaagaata tggatgtcga
    44101 atggggctat tctggagaca aaggtccgga aaattgggcg agtttatgtg attggtttgc
    44161 gagaggtgcg gaatttccat tgcagtcgcc tattcatttg accaaggggg aagaaaccaa
    44221 aatagaagga gacacgcttt cctttcatta tcaaaagcaa cggtttactg acaaggaatt
    44281 caaaaacacg attcatttag taccttttga ccaactcagt tatgtagagt tcaagaaaga
    44341 acgctattat ttgacagata ttcatttcca cttgccttct gaacatatca tcaacggaaa
    44401 acaggaagat attgaatttc attttgttca tatgaacagc aaaaaagaga atctagttgt
    44461 gggtgtcatg taccagctaa tggtacagga aggttggctg tataatcaag aaaatgggga
    44521 aagttggaac ttagaaaaac atgagcattg ggtaaatccg gatgtgttct ttccggagca
    44581 aaaaagccat ttccattata ttggctcatt gacaactccg cctaccagcg ggccaatcca
    44641 atggtttgta atggatcatg taggaaaact gaaccaagag tttcttctcc ccttcgatgg
    44701 tcagtatgct cgaccgaaca accgaccaat ccagcccttg aatggaagag aaatttatta
    44761 tttcgaggag tttgagcaat gaatattcag cagatgaaat acgtggtcgc tgtagcaaac
    44821 aatggtagtt tcagagaagc ggcaaagaaa atgtttgtct cccagccaag tctttcagct
    44881 ggtatcaaag aattagaaac cgagttaggg atcacgctgt ttactcgaac gaatcgaggg
    44941 gcatatttaa cagaagaagg gcaagacttc ttaaatcgtg ccgagaaaat attggtccag
    45001 cttgatacat tagaaactca ctatctaacg agtgaaaaga gagacaattt ttcgatcgct
    45061 gcccagcatt atgatttctt aggtcctttg accgcacagc tactcgagga attcaaagac
    45121 gagatcaaac aatttcggat catggagacg acaacagcga gagtgatcga agaagtcaaa
    45181 gagcagcaca gtgagatcgg cattatttat atgaatgagc ataatcaagc tggtatcaga
    45241 agatatttac aacaaggaga gctaagtggc cgcagcattg gaaaattcaa aacgcatatt
    45301 tttttaaggc aaggacatcc attagctgaa cacgcagaga tcaccaaaga agaattgtta
    45361 tcttatcctc aagttcgttt tacacaggat ggaaataatt tcccgtattt ttatgaagac
    45421 cttatcgaga tccctgatca agaaacagtg atttatacga gtgatcgagg tactttgatg
    45481 aatatcgttc ttgaaactga tgcttacgct tcaggatctg gtattgtgat tggagggatt
    45541 cgcgagcact tgcggttaat ccctttggca gaaggcgaac taaatgaatt ttatatcatt
    45601 tattcggcga aacgtccgct cagtgaaatc gctcaaagat ttatcaaaga gcttactcgg
    45661 ttactggacc agcagagcta agaaaagcag atgagaacgc tgtttattga acaccgttga
    45721 tcaaaaaaga ggggttgtga cagaattt
//
LOCUS       DACGLW010000012        44605 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.012, whole genome shotgun sequence.
ACCESSION   DACGLW010000012 DACGLW010000000
VERSION     DACGLW010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44605
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.012"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            complement(5..586)
                     /locus_tag="IXT53_000750"
     CDS             complement(5..586)
                     /locus_tag="IXT53_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ4904600.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(769..1395)
                     /locus_tag="IXT53_000751"
     CDS             complement(769..1395)
                     /locus_tag="IXT53_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ4904601.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(1417..2748)
                     /locus_tag="IXT53_000752"
     CDS             complement(1417..2748)
                     /locus_tag="IXT53_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HAQ4904602.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(2903..3304)
                     /locus_tag="IXT53_000753"
     CDS             complement(2903..3304)
                     /locus_tag="IXT53_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HAQ4904603.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(3390..3596)
                     /locus_tag="IXT53_000754"
     CDS             complement(3390..3596)
                     /locus_tag="IXT53_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4904604.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(3691..5193)
                     /locus_tag="IXT53_000755"
     CDS             complement(3691..5193)
                     /locus_tag="IXT53_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HAQ4904605.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(5211..5666)
                     /gene="argR"
                     /locus_tag="IXT53_000756"
     CDS             complement(5211..5666)
                     /gene="argR"
                     /locus_tag="IXT53_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HAQ4904606.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(5764..6381)
                     /locus_tag="IXT53_000757"
     CDS             complement(5764..6381)
                     /locus_tag="IXT53_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4904607.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            6602..7975
                     /locus_tag="IXT53_000758"
     CDS             6602..7975
                     /locus_tag="IXT53_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ4904608.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            8050..9162
                     /locus_tag="IXT53_000759"
     CDS             8050..9162
                     /locus_tag="IXT53_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HAQ4904609.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(9234..10871)
                     /locus_tag="IXT53_000760"
     CDS             complement(9234..10871)
                     /locus_tag="IXT53_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HAQ4904610.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAVVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(11039..11647)
                     /locus_tag="IXT53_000761"
     CDS             complement(11039..11647)
                     /locus_tag="IXT53_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ4904611.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            complement(12031..13182)
                     /gene="queG"
                     /locus_tag="IXT53_000762"
     CDS             complement(12031..13182)
                     /gene="queG"
                     /locus_tag="IXT53_000762"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HAQ4904612.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(13201..13953)
                     /locus_tag="IXT53_000763"
     CDS             complement(13201..13953)
                     /locus_tag="IXT53_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4904613.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(14094..15044)
                     /locus_tag="IXT53_000764"
     CDS             complement(14094..15044)
                     /locus_tag="IXT53_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HAQ4904614.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            complement(15045..15884)
                     /locus_tag="IXT53_000765"
     CDS             complement(15045..15884)
                     /locus_tag="IXT53_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ4904615.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(15881..16627)
                     /locus_tag="IXT53_000766"
     CDS             complement(15881..16627)
                     /locus_tag="IXT53_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ4904616.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            16865..18235
                     /locus_tag="IXT53_000767"
     CDS             16865..18235
                     /locus_tag="IXT53_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ4904617.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(18290..19186)
                     /locus_tag="IXT53_000768"
     CDS             complement(18290..19186)
                     /locus_tag="IXT53_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ4904618.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(19467..20144)
                     /locus_tag="IXT53_000769"
     CDS             complement(19467..20144)
                     /locus_tag="IXT53_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ4904619.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(20235..21140)
                     /locus_tag="IXT53_000770"
     CDS             complement(20235..21140)
                     /locus_tag="IXT53_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ4904620.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            21315..22769
                     /locus_tag="IXT53_000771"
     CDS             21315..22769
                     /locus_tag="IXT53_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ4904621.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVVPSH"
     gene            22785..23816
                     /locus_tag="IXT53_000772"
     CDS             22785..23816
                     /locus_tag="IXT53_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ4904622.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGHFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            23823..25010
                     /locus_tag="IXT53_000773"
     CDS             23823..25010
                     /locus_tag="IXT53_000773"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HAQ4904623.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            25166..26548
                     /locus_tag="IXT53_000774"
     CDS             25166..26548
                     /locus_tag="IXT53_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAQ4904624.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            26520..26906
                     /locus_tag="IXT53_000775"
     CDS             26520..26906
                     /locus_tag="IXT53_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4904625.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(27106..27774)
                     /locus_tag="IXT53_000776"
     CDS             complement(27106..27774)
                     /locus_tag="IXT53_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HAQ4904626.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(27963..28760)
                     /locus_tag="IXT53_000777"
                     /pseudo
     CDS             complement(27963..28760)
                     /locus_tag="IXT53_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(29041..30420)
                     /locus_tag="IXT53_000778"
     CDS             complement(29041..30420)
                     /locus_tag="IXT53_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ4904627.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            complement(30595..31764)
                     /locus_tag="IXT53_000779"
     CDS             complement(30595..31764)
                     /locus_tag="IXT53_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ4904628.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            complement(31739..32890)
                     /locus_tag="IXT53_000780"
     CDS             complement(31739..32890)
                     /locus_tag="IXT53_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HAQ4904629.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            complement(32850..34211)
                     /locus_tag="IXT53_000781"
     CDS             complement(32850..34211)
                     /locus_tag="IXT53_000781"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HAQ4904630.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            complement(34208..35209)
                     /locus_tag="IXT53_000782"
     CDS             complement(34208..35209)
                     /locus_tag="IXT53_000782"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HAQ4904631.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            complement(35225..35920)
                     /gene="dapD"
                     /locus_tag="IXT53_000783"
     CDS             complement(35225..35920)
                     /gene="dapD"
                     /locus_tag="IXT53_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HAQ4904632.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            complement(35926..36642)
                     /locus_tag="IXT53_000784"
     CDS             complement(35926..36642)
                     /locus_tag="IXT53_000784"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HAQ4904633.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            complement(36639..37520)
                     /locus_tag="IXT53_000785"
     CDS             complement(36639..37520)
                     /locus_tag="IXT53_000785"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HAQ4904634.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            complement(37523..38323)
                     /gene="dapF"
                     /locus_tag="IXT53_000786"
     CDS             complement(37523..38323)
                     /gene="dapF"
                     /locus_tag="IXT53_000786"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HAQ4904635.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            complement(38310..39569)
                     /gene="lysA"
                     /locus_tag="IXT53_000787"
     CDS             complement(38310..39569)
                     /gene="lysA"
                     /locus_tag="IXT53_000787"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HAQ4904636.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            complement(40055..40618)
                     /gene="efp"
                     /locus_tag="IXT53_000788"
     CDS             complement(40055..40618)
                     /gene="efp"
                     /locus_tag="IXT53_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HAQ4904637.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            41046..41963
                     /locus_tag="IXT53_000789"
     CDS             41046..41963
                     /locus_tag="IXT53_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HAQ4904638.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            41976..43121
                     /locus_tag="IXT53_000790"
     CDS             41976..43121
                     /locus_tag="IXT53_000790"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HAQ4904639.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            43292..43750
                     /locus_tag="IXT53_000791"
     CDS             43292..43750
                     /locus_tag="IXT53_000791"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HAQ4904640.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            43909..44379
                     /locus_tag="IXT53_000792"
     CDS             43909..44379
                     /locus_tag="IXT53_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HAQ4904641.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(44432..44547)
                     /gene="rrf"
                     /locus_tag="IXT53_000793"
     rRNA            complement(44432..44547)
                     /gene="rrf"
                     /locus_tag="IXT53_000793"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT        12497 a         9256 c         8078 g        14774 t
ORIGIN      
        1 attattaaag aaccttttgc gaagttaaca atctttcttt gatcaattga aaattctgcc
       61 taagtagtga tagatcctgt gtgactttca acaataagat acttccctgg atttcagcaa
      121 tcaacgcttt ggcttgacgg tctgcttctt catcggacag tttttcttca gaaagtgcca
      181 ggatattttg tttcgtatac ttgacccatt gattgactaa ttctagtagc aacagacggt
      241 attcttcatc cactgtactc aactcaataa tcaaattacc tacaggacag ccatgataag
      301 tgatctgtgt ctcatgatca gcgaaaatcc agtccaacat ttcttctaat gcttcttctg
      361 gctgttttcc agcatttaag atgtcttcga acaattctgt ctgccatacg tccatatgcg
      421 catggatcac cgctagacaa acttcttttt ttgaagaaaa gtaataataa aattgacctt
      481 ttcctgtatc agaagctgct agaatatctc ggattgacgt ttcatgatag ccttttttat
      541 aaataagatc tttggtcgct tccaagatct tttcctttgt tttcaatgat attctccttt
      601 ttcatttctc atagtcctat attatacagt aaattcatca ttttttcttc ttaatcgttt
      661 aaaaaagact gtgccaaatt atctgacaca gccctcacta taaaactgtt ctcatatagc
      721 tagatgttca ttgattcaag aactgcgagg acaaaagcaa ttcctatctt atgatagttg
      781 ctctaatact tcctctgttg tcatcacttg atctgcaata aagccaaagt tcgtcaacgc
      841 tgcatccatt gcttcttggc caggagcacc aattgcatct gtcgctacca caacattgaa
      901 tccactctct accaattcac gcatatggga atcgctacac aagtttccgt tcattccacc
      961 aagaattact gtgtcgattc cattttttct caattggagc gccaaatcat tgctttctgg
     1021 tccaaagatt ttatgaggag agctgatgat cgtattttca tccaaataag gtttcaattc
     1081 ttcgataaaa tcggctcctg aaccttcttc cactgattca tattgacttt ttctagcaaa
     1141 catctgctgc tcaagcatca tatgttctcc tgcaccgcca aattgccatt tatggtcatg
     1201 tgggtaaaaa taatgaggag aaataaagag tttgtatcct ttttcttttg cttgttttac
     1261 catttcaatc atattttcga tcgtattgta tttttctaag atatcttttg tcaaaccaaa
     1321 gccaccgcct gctggtttca agaactcgac ttgcgggtca gtgagaacta ctgctgtatt
     1381 tttattattg aacatggtgg attctccatt cgttatttat tttaatacat catttagagc
     1441 ttcactcatt gtcggatgag aatagatttg atctctcaat aacgtataag gaagttttcc
     1501 attcatagct agcgaaatca agttgatgac ttcatgagaa tcttccgcat agatacttgc
     1561 ccctagaatc aagtcagttt cagggtcgac taaaactttg aatacacctt taggatcttc
     1621 taatacttgt gctttaggga caccgcttgt catatacttg aataatttgt aattgacgcc
     1681 ttgtgctttg gcttgttttt ctgttaaacc tacattggat aaagtcggag tgataaatac
     1741 actgtaaggc acagctttcc ggttattcgt tgttcgttta ttctcgccct tcaactgatc
     1801 cataatgatt cgataatcat ctagagagat ataagtgaac tgcaatccgc ctttgacgtc
     1861 tccaatcgcc caaatgttgt ctgcatctgt acgaaggtat tcatccacag cgattgctcc
     1921 gcgatctgtt agtttaacat ctgtattttc taatccaagt gatgctgtac ttggttttct
     1981 tccagttgca gccaaaactt ttgacgcttg gattgttact tcttggccat cttctgtaaa
     2041 ggtgacggca gctgttgatt cttggtctgc tacttgatct acagatacac ctagatggaa
     2101 ttcgattccc gcatttgtca tgtcttctaa aatcatttgc gcgatatctt catcttcacg
     2161 tggaagaaat tcagaatggg catcaagaac gacaacttta gatccgtatt cattgaacat
     2221 tgatgcaaat tccaagccaa tatatccagc cccgatgatc actaattttt ccggtaattg
     2281 ttcctgatcc atcgcttgcg tagaatcaat caagtagcga cttgtattca atcctttgat
     2341 tggtaaaatg attggttcag aaccagtatt gatgaaaatc cgttcgcctt caacaatcac
     2401 tttttcacca ttattcgtca cttcgatttg atggttagaa agaaaacgtg ctgtaccgtc
     2461 catcactgtc ccagttgcct catcgctgat catatgataa tttttggcgc gaagcatgcc
     2521 cgtcaatttt tctttccgag caacagcctc tgtaaagtcg attcctcttt ctccattgaa
     2581 aatcaatgat ttagaaggaa tacagccgat gttgatacat gttccgccat acatacgtgt
     2641 tgacttttca acgactaaaa ccgattcacc ttttgaagct aacgccttcg ctaaagtctt
     2701 accgcccttt ccaaaaccga tcacaatatt ttgatatttt tccatcattt ttccctcgct
     2761 tcttttttac ctaccgattg gtacaaaaat atgataaccg ataattgtct attctccaaa
     2821 tcatctgctt tatttgtcaa aaaaaaccag acaaaaagcg gtcctttcac gtttttagtc
     2881 tggttcgatt tcctttattc ttctaaacaa tcgttacttc tacatcgata tttcctctag
     2941 ttgctttgga gtaagggcag aattcatgcg catcttctgc aattttttgg gcttcttctt
     3001 cgctgatgcc atcgatatgc acttctaata ctacaccgat ctgatagccg ccatcatcta
     3061 aattataaag agagacacgt gcttttactg tggactctgc tgtcattcct gcttctttga
     3121 tcatatgttg taccgcccca ttgaaacaag aagcataacc tgctgcaaat aattgttctg
     3181 gatttgttcc ttctgcatgg attcctggag gagtgatttt catatgcatc gagccatttg
     3241 gtgcctcgac ttctccattg cgcccgccat ggttgatcat tgctgtttcg tatgcttttt
     3301 tcattgtact gctcctttcg attgaccatt tgctacttgg ctataattat agtgtatgac
     3361 aagtaaggcg ctttctacaa aaattctgct tattcgaata ccagaatgcc gtcttctact
     3421 atttttccaa aaaacaaaaa aatactactc gtgaacaaca gcgtcatatt tgttccaaaa
     3481 taaatagcaa tcttttctac ataatctttt atatgattga ttttaagata catatatacc
     3541 agaaagttga ccccataaac caatactgtt aaaaaaataa ccatattcgt taccattttt
     3601 tcctcctttt gctggtctcc ctaccagcac tcgatttaac cataccactt tttttgccgt
     3661 ccaagcaact agcctttcaa aaagactggt ttattctgct gttaatgatt caagatcaga
     3721 aaaatgtccg aataattttg ctaattttac taacgccaac ttgttctttc cgaattcatc
     3781 attttctatc tgtaatacta gtaacggctc atgtaagctt aaacggatca aaaaccagcc
     3841 gtttccgtat ggctcttcta gatcatatcg aataccttct tcattttccg ggtcgaagtt
     3901 catccctttt tcttcttcca acagtcgctt catttcttct aatatcttgt ttccttgttc
     3961 gcgtggttca tcgctgaaca attttaatcg gatttcttgt acttctacgg gctgtttcaa
     4021 ctctttgatt tgttcatcta atgtgatacc ttttcttttt aattctggca aaagcatcag
     4081 gatctttgcg atgacatatg caccatcatc cagaaaatag ttttccttga atgcagcgtg
     4141 tccgcttgtt tcgatggcta gctgtgtatc tttcccaaga cggttaagtt ctaatgcttt
     4201 attgattaca tttcgatagc cggaaatata acgaacttgg atgcctcctt tttcttcaat
     4261 gaatctttgt agatgagaag aagtagggga attcgtcacg attcttgttc ctggatgctc
     4321 ttttaatacg attgtactta atactgcgat caaattattt cgattgatca ctgttccaga
     4381 agcactgaca actgcagaac gatccacatc tgtatcgaaa atgatcccaa gatctgcttc
     4441 atttgctaaa acagcctgct tgatactttc catcgcttct ttattatcag gatttgggat
     4501 atgattagga aatcggccat ctggttccaa aaattgggaa cctgttgaat ccgcacctaa
     4561 gctattcaat actttttcag caaagaatcc accagcacca tttccggcat caacaatgat
     4621 tttccaacca tttagtggtt tgctttctag tgttccaatc ccctttttaa tttttgtgac
     4681 taaatcttgc gcataaggtg tcaaaaggtc tgctttttct acgcttccag tttgttcgct
     4741 ttttattttt tgctgctgtg ataggataaa agcaatatct tctttttctg ctcctccttc
     4801 tgaggtaaag aattttattc cattataata atagggaaga tggctagcag tcagcataac
     4861 cgcagcatcg cacgagaact gttcaaattg agtggacata aacatagcag gcgtagtagc
     4921 taattcgaaa tcgatgacat gtatcccttg ttctttcaga ctttggatca acgcctcttt
     4981 cagtgctggt ccgcttatcc tactgtcatg accaatccca atcgtccatt ttttatttgg
     5041 atcggttgat ttcttctttt tctttatcca gtttgctagt ccccagccga tcttttttac
     5101 catgtctgtc gtcaagttta cttcatatcc ttcaactggt aatgcgattc ctcgaatatc
     5161 cgatccattt tgcagcgctt tcatatcttt catataaaag tcctcctcat ttattccctc
     5221 attttttcca gcttttctgc aaatcgctgt gcttcttctt ctgagcggca aataataatg
     5281 aatgtatcta caccggccat cgttccgaca acttcagggt aagccacttc atctagccaa
     5341 ttcgttacca catcggcacc atttcgttca gtcaatacga taaccatgaa ctgaacagac
     5401 tggatacgca aaacctcgcg tctaattgct gtacgcaaac gttcttcatt taaagaaaca
     5461 gatggctggt taaataatgt atagcgtact attttgtttt cattttgact ttttactaac
     5521 ttcatttctc gaatgtctcg agaaatcgtt gcttgagtca catcggcgcc tgcttcatgc
     5581 agccgcgcaa gcagctcttc ctgtgtccca ataggttgtt ctgtaatgat ctggcggatc
     5641 agtgcttgtc gctgtttctt tttcatatta gacacctctc aatgttgatt atagcaaaag
     5701 ataagtaata tttcttcata ttttggtatt ttttactttt attttttgtt ttcattcagg
     5761 ttgctaacct ttttctattt taacacgcaa aatcgtcttc attttttcag gtgctgattt
     5821 tcttggatcg ctgatgtaaa tctctttgtg ttctttagaa gctctttcat agccttcttt
     5881 tgcgatgaat gcttctaatt tctcaaacgt tgctggttct tcggcatagg gcccgacgtg
     5941 taagatttgg gcacaaagac cttcttccat ctttttgaat actacttgtt ctttcaagtc
     6001 ttccggaagt ttgctagaac gctgaatggc ttcttgtgct atcgcttctg taacaaagtc
     6061 aggctgtcgg atcatcaatt gataagaaaa atgttctttt ttcatcactg gttcatgcaa
     6121 atacttttct tgcagtcccc attgtccttc cagtggatag acagtataca tctgataatc
     6181 aggaatcagc caattttttt taggcgacat gcgtattgca tagctgactg catatagaca
     6241 ggctactctt cgtttaaagt cttcctgatt cgggtcgccg atccctctga tgctaaagaa
     6301 tgatttttta ggaacctcta gtatttgcgg gctttttttc atactatagc tcttgtcttt
     6361 acgccattca attttttcca tttttcttcc tccgcgaaat tccatttagt tatttattat
     6421 catacctctt tttgagtgac ttggctaatc gtgttgacac tatttttcag tttgtgctag
     6481 aataagtgaa gtaaataaat cgtacagata acacgtttcg gcgtgtcgag agagaggcgg
     6541 tttctatttt tataatagaa atcactctct ttttttacga aattttttag aggtgatgta
     6601 aatgacaaaa caaaaacagc tgcttttagt acctgcagta attgcaacag ggatgatgtc
     6661 ttttgcgggc gtattgatcg agacggccat gaatgtaact ttcccaactc ttatcagaca
     6721 gtttgattta acgactgcac aagtccaatg ggtaacaacg atttatcttt tgatgatttc
     6781 gatcattgtt ccgctgtcta cttatttgaa tcgcaatttt agtctcaaaa aactcttttt
     6841 agcttcaaat ctgcttttct tggcaggtat aatgatcgat ttcttctctc ctaccttcat
     6901 ggtcctattg tttggtcgat tgctccaagg tgcagcgaca ggtatcgctc ttccgttgat
     6961 gttccatatt atcttgacat ttgcccccct caatcgacga ggcgcgatga tgggtgtcgg
     7021 tactttgaca acggccattg ctccagcaat tggccccact tatggaggca ttatgacttc
     7081 acattttaca tggaatcata tcttcctgtt tcttgtgccc gttttgattc tttctttgat
     7141 tatcggctgg tttgctattc ccgagatggc agtacaaaaa agtggttatt tggattggat
     7201 aagcgtttta ggaatttgtc ttttgtttag tggaagtcta acatttttga gtacgttggg
     7261 aacagtttcc agctggattg ctttaatcat cggcggtatt ggctggttcc ttttttacta
     7321 tcgaagtaaa aaagtggagc atccactgat ccgactgtct attctttcaa ataaaacatt
     7381 ccgcttgttt ttgattggct tcttagttta tcagttttta ttacttggtg tatcttttgt
     7441 cttgccgaat ttccttcaga tcgttcaagg aagtagtcct tttgtcgctg gattagcgat
     7501 gcttcccggc gcagcggttg gtgctttgct ttctcctttt tccgggaaaa tgctggatca
     7561 atatggtccg aagaagccaa tttttgctgg gctgattctt tctttgattg gttggctagc
     7621 attattccta ctcataggac acgcttcagt gggtgtattg attgcttgtc atgtgtttta
     7681 tatgatcggt atcggattat cttacagcaa tatgatgacc accggaatga atgcactgaa
     7741 tgaagaacta caaggagacg ggaacgcaat gttcaatacc ttgcagcagt tttctggcgc
     7801 tgtggctact tcattagtag cggtcattat caattacgtc cagcatcata cttcccataa
     7861 ctatgaagtg tctactacat taggatcaaa agcagcatta ggcgtcttgt tgctgttgct
     7921 tctcgtaagc tttgctcgat tcgcctatta tctgttcttt gctaaaaaag cgtagattcc
     7981 atatacggca ggtgttgcat ctgccgtttt tttgtgtgaa aatggttgtt tgtaaaagga
     8041 ggctttttta tggaaaaaat tgccattatc ggtggcggga tcattggtat gaccctagcc
     8101 agttacttag atacatcaaa attcaatgtt gctttatttg atgaagggac aggacaggca
     8161 actaaagcta gcgctgggat tatctcccct tggttgtcga aacgcagaaa caaacaatgg
     8221 tatcgtctgg ctaaagacgg cgctgctttt ttccctaaac ttataaaaga tttccaactt
     8281 gatcaaacga tttacgcaca atcagggaca cttttgcttc gacctgaaaa agatttagct
     8341 gaattggctg ttcttgccga gaaacgaaaa gaagaagcgc cagaaattgg aaacatcgaa
     8401 tggctttcac ctgaacagac atctcaagca cttccgttat tgaaaccagc agtttcactg
     8461 cgtatcagtg gtggtggtcg tctggatgga aaagcatatt tagaacatct tggtcagcgt
     8521 ttagcacaaa aacaaatgga gcagatccag gcaaaagtca ctttgcataa gaagagcgat
     8581 tcttggctca tcaagggacc gtttggagaa tacacagctg atcgtgtaat tttgacacct
     8641 ggcccggcac ttaagccttt attacaagaa ttaggctatg ctgcagatat acgtccgcaa
     8701 aaagggcagc tcttagcatt tcagacatct tataagatga gtcaagactg gcctgttgct
     8761 atgcttgacg gagaagctga tttcattcct ttcatggatg gaaagatcct tttaggtgct
     8821 acccatgaaa acgatcaagg ctgggatttg gctcctacta gcgaagctta taagggattg
     8881 actaagagta cagaacagtt cttgactgat ccagatcaga taaaaaactg gccatacgag
     8941 ttccgtgtag gtacccgtgc ttatacgtca gatttttccc catttttcgg cgtactgcca
     9001 gatgatccaa gcttactggc tgctagcggt ctaggctctt ctggcttgac cacaggtcct
     9061 tatatcggct atctattagc caacttctta aatactggtc attggcctac ggaagattat
     9121 caaaaggaca tctccaccta tattacgaaa aatccatcat agaaaaacag ctttttcaag
     9181 taaggatccc gagcgatatt gcgcatggat tacttcttga aaaagctgtt attttagttc
     9241 aatttctgaa tcaaattctc caaactttct tttgcttcat ctggcaagag atcttttcct
     9301 atctgagctt caatgaatcg taaacgaata tcaagtcgtt gtttcaccag atttttatag
     9361 gacgtatcct caaaagaaac aggaaaatct ggtataggta aatagtggat gtcttcaaat
     9421 ggaccaaacg acttgaattc tgccgtttgt tgcacgattc tttcaacgat tgacaatgtc
     9481 gtttcaggtt tgattttttt atcatcgaaa tatccagtat tcaggacata gcaggctgtt
     9541 tgctgtttca aaaacaactc tcgaaaagct tgatagtctt ccctaagagg atagacacgg
     9601 aaaggattag caaatggttc aatgactagc tcatctagct gtctttttgt agtgatattt
     9661 tccgcgtctg atcgttttgt tgctaatgtt gcaccaaaag acgctgctaa aactgggtca
     9721 ttgatcttga taattggcgg aagcgaatca tctttcatga tccaaaaaac agcatcgatc
     9781 ttttcgctaa gatgatcgac tcggtcgggt gtcgcataac gtgatttgac tgtccgccca
     9841 tttccgtttc gtatatctcc tgttactagg actttttctc ctttacttgt acatgtcacg
     9901 cccacattct gacaagtaag gaaagaagaa atggccggat catcagacgg atagtcttgt
     9961 gttttatcaa agtaagaagg ttccaaggca gtagcaaaac cattttttcg agaaataacg
    10021 aaagcatcgt catgcaagat cgaaacagag tgaccttccg tatgttttgc taaggtaatc
    10081 gttgattttc cagaaccgga caacccaaat acagccatcg tataggtctg gtcattacta
    10141 gcatattgtt tcatcccacc gtggcaggca ataaagccat tgcgatttcc caatgcccaa
    10201 gcaagcgtca aagtcgcttt ttttaattct ccaaagtaac gtaatcctag cacaaccgct
    10261 gtattatgga gagcatcaaa caaaatgagt ccatttggaa atgtttcatg tgtccattcg
    10321 ggatcacaaa agatataaag atctccctca tcaaaggaaa cagacttttg ataagtctct
    10381 aagatcttcg aattcgctgg ctgaaaattc agcaaataag aatacaaatt gttctcaaat
    10441 ccttctggaa tcagcaaatg actttgcaca gaaaattctt cagataggcc aacgattact
    10501 tttgtatggt aaaactggcg cttgcgactt tgatacacag cttctcttaa aattttctcg
    10561 tatttgcttt gttccttgtc taagtcctca aagatccgcc gagcacttgc tgttcttccc
    10621 acaatgcttc catcattgaa tactagtgtc ctaacatcag ctggtaaatc tagttcttca
    10681 gcatgtgaga ttgttaaatc tgtgatgatc gttcctggac tcccttttgc tagttgataa
    10741 gctgtcttcc tatcaaaaac ttcatgaaca ttattaccgt aaaatgtcgt ttctacaatc
    10801 gatcgaatac tggataacag tggatttcca cttgtgattt cgtttaattc gagactctct
    10861 tttgtactca tggctcctcc tcattcccta aacaaaaaaa cgttttcttt attatacatt
    10921 attcgagttt ttatttgtac tgctgcgctg tgggaagcaa acgagttatt tttcacacaa
    10981 aaaaaccgag aacttgaata aacaagtttc tcggtttcat cattttcctt ttatcggctt
    11041 agtaccagcc gtttgctaac cagaatgttt tagcagcatc ccatgaacca taacgagatg
    11101 ttacatattg ttctgctaca cgttcttgat tggcagctga atagtcgccg tttaagtatg
    11161 aagcatctaa ttgataacgg ccgatgtaac gtccgttagt agctgtgtat gaaccgcctg
    11221 attctttttg tgcgatccat tcttttgcag agcttgaagt tggcgctgtt gcagcagctg
    11281 gggcagcagg agctacttca actgtttcaa caggtgcaac gtatgtatgt tcttgcgttg
    11341 ctgtttcagt tgtagatgct gtattttctg ttgttttgtt ttctgttgtt gctgaagaat
    11401 catttgaagt tgggattgtt aattgttcac caacataaat gcggttgatg tttgcgatcg
    11461 aatttttttc agcaattgca tcgatcattg agttgtctcc tgcaaatttt tgtgagatct
    11521 tagataatga atcacctgat tttactgtat aaacttcatc agcgtgagca tttgtagttc
    11581 ctacaaacat tgcagtagca ccagcagcaa aagttgtacc taaaacaatt ttcttaaatg
    11641 aattcatatt aaactccttt ttttctccgt tttttattct ttcgacgcta ttaactttat
    11701 catttagaaa tatttcatag gtaaaagaaa catgacattt aatgacaaaa taacgaatta
    11761 tttaatattt gtaacattta aacaaatatt tttttgatac aaaaccataa aagccttgtt
    11821 gtataagcaa agtaatcgtt tttctatgtt tttttatttt tgaatgaaaa acgcagattc
    11881 tgtaacattc actgcaataa tccataaaaa aaagcaaaaa aatttttgat ttttatcaaa
    11941 ttctcttaaa aaaatgtaaa aaatacaaaa aaaccagtaa ttgttacaac aattactgga
    12001 caaaaacttt gaataatgtt aactattttt ttattttaac tttctccgca ataatttctg
    12061 ggcatgttcc aattcgttta taacgacttc ttctgtttct ttagctaata tttcatctaa
    12121 tacatcaagc catttttccg gttcttttgt tcctagacgt ccaatagccc aagctgctgt
    12181 tcctctgatg acagggcgga catctgttaa acattctgaa atctcaggca atgccgttcg
    12241 atcacctaga ttagctaaag caatcaatgc gtttctctgc aaaggtttct tcccacgcca
    12301 tgaaccagct aaatgcccaa attgctgttt gaagtcttta ttagacatcg aaagcattgg
    12361 ctttaatttt ggatatattt cgtctacttt tggttccatc tcttcatgaa aatgaaaatc
    12421 ttttccttga ttgtaagggc agactaactg gcagatatca catccataaa tgacatttcg
    12481 cattttttta cgatattctt ccggcatcat cccttttgtc tgagtttgat aagacaaaca
    12541 tcgctgggca ttcattcttc cgtcaccaag caatgctttt gtcggacaag cagtcacgca
    12601 gcgcatacag tcaccgcaac caaattctcc tggtttatca ggctcaaata caatattagt
    12661 aacgatctca ccaagataga caaatgaacc gaactcttca gtgatcaaca atccgtttcg
    12721 tccaataaat cctaaaccag ctcgctgtgc tacagcgaca tcgatcagtt ctccagtatc
    12781 tacttgcgga gctagacgcc attcctcttc tttttgccat ttttctgctc ttgtttggat
    12841 gaattcgatc aatcggttca aacggtctct gagtatatca tggtaatcga tcccccaaga
    12901 agcacgagca aactgcccgc gtttttcatc cctaggcact tcttcatgga tcctagtagg
    12961 atacgcaagt gcaatggaaa tgatcgattt cggctgatca aatgtcattt ctggataaag
    13021 acgctcatcg atatttggat gttcaaagcc agaagtatgg ccagctgctt tttgttcttc
    13081 caacgattct tttaatgaat caaaaggttc cgccgtggtg aacccgattt tgtcaattcc
    13141 taatcgtttg ctttcttgta tgatttcttc ttttaatgtc atggtcttcc tcgcttgctg
    13201 ttaacggctg tataacccat aatatagttt ttcttttttc agctggacaa actggatctg
    13261 ctgatcggca atcttctttt cttccatcca tttttttctt gcagcaaata cttcccaata
    13321 atttgttgaa attaattttc ttttgttatc taatgcatta aaaaaattca atgaaactgg
    13381 ttttgtactt aacagttcat ggagccttac ctcttcccat ggcgaaaact cagacagatc
    13441 gcgggaatac aatacttctt catagagatt tttttgctgt gtgtacgtga atgtttgcgg
    13501 gattttccca ggcctagcaa tttctactag acgattataa aacatattca ttaatttgct
    13561 gttggcaata aattgacttt tataataggc cgactggatc tttttgatta ctccttctgc
    13621 tttgatgact gaaaataatg caggccatgc tttgacggtt atttcgtcgt tttgtttaac
    13681 taatacgcca ggtgtgaagc cgactggaga aaggaacaca aaataaccga taccggtatt
    13741 ttcatctaaa ggctgaaatt catcaaacaa ccatttgcct atgtacagct cttcatctgg
    13801 ttctgttgtc tcattttgat ttgctatgaa taatttagct aattctgctt caagaaattg
    13861 aaggtcttga tccttgtcgg tattttcctg ctggctgatc aatcgaaaga cttcttcttt
    13921 aatcgtatat ttatctttac gtgtaaaatt catagccaca ccttccttcc ttactctaac
    13981 tataacggaa tttcttcaaa aaaggtatct gtacgacgaa aaacagcata attttttttc
    14041 atatctctta taaaaaatgc aaggtctgct cctcaagcta gaccttgcac ttttcagctt
    14101 tcttttgttt gtgacaaacc ttcatggatc gtttcaatat tccatttcat catctgatag
    14161 taagaatctc cttgttctcc ttcttttgct atggaatcag tgaacagttt atcatagatt
    14221 ttcaaacccg tttcttttgc tacccgttcc atgcttcttg agtctacact ggtttccacg
    14281 aataaaaccg gtacttcttt tgctcttatc tgatcaatga tcgctttcat ttgatcaggc
    14341 gtaccttgac tttctgtatt gatctcccat atataagctg ctggcaaatc atatgctcgt
    14401 gaaaaatact tgaatgcccc ttcacttgtt actaataatt ttttgttctc ttcgatagaa
    14461 gcaaaacttt ccttcgcttg tgtatctaat tcttttagtt tttccacata tgcttctgca
    14521 ttttttttat agagtgtcgc attttctgca tctttttcag agaatatacg tgcgatttcc
    14581 tccacatatt ttattccgtt agataggtct agccaagcat ggggatctgc ttttgtatgt
    14641 tcttcaccgc tagttaaata aagaggttct acatttttgc taactgcaaa ataatctttt
    14701 ccttcttctt ttttagccgt ttccatcaag ttatcgaacc agctgttacc tgtttcaaga
    14761 ttcaaaccgt tgtataaaat aacatctgca tcgcttgcct ttttgatgtc ttctggtagt
    14821 acttcatatt catgcggatc tgttccgact ggtacgatac tgtggatatc tatttgatct
    14881 gttccgacct cttttgccat gtccgccaag atcgaattgg tagccacgac tgacaacttt
    14941 tctttacttc cttcatccgc ttggtcagaa gcctgctttc cgcaaccggc taataccaag
    15001 aaactaactg ccaagattaa aaagagtgat ttttttgttt tcatctatct gcttcctttc
    15061 tgaaataaaa ttccttttgt tggagaaaat aaaaaagcaa ggataaaaaa tgcggtacat
    15121 gctaacacga tcgtagctcc tgatggccaa ttgtaactat aactgaaaaa cacaccgaca
    15181 actgcactta gcataccgat tcctgcagct gtaatgatca ttttcagtaa atggttcgtt
    15241 agcaagtaag ccgtggcggc tggtgtgatc aacatggcaa tcaccaagat tgttcctact
    15301 gtctgtaaac tgactacagc cactaatgtc aaaaagaaca tcaaaagata atggatcagc
    15361 gatgtgttca gcccataagc ttttgccatt gtcggatcga atgatgtgat cttcaattct
    15421 ttataaaaga aaaagacaaa tatcaagacg attccactaa cgagtgctgt cagcaacaaa
    15481 tcgctttccc taacagctaa gacattccca aataaaatat gatacaaatc tgtcgcactc
    15541 tgggcatatg aaatcaagat aatccctaat gcaaaaaagg agctaaagac aattccaatc
    15601 gctgtatcat ttttcaatcg gctccgctga gtcacgaaac caatcactgc tgctgttagc
    15661 atcccaaaag cagtggctcc aaagatgtaa ctaaaaccaa acatataaga aatggctaca
    15721 cctggaagaa ctgcgtgaga gatagcgtct cccatcaaag acatccctcg taaaataata
    15781 aaagaaccga tgactccaga agcaagtcct acaagcatgg aagtgaccaa agcgtattgc
    15841 aggaattgat aattcattaa tccatcaata aaggattgga tcattttttc tctcctcctt
    15901 ttactaaatg tctgatgatc tcaccatagg ccaattgaag gttcttcgat gtaaagactt
    15961 cttgtactgg accggaagcg atcagctgtt tatttaaaag aataacctca tcgaaatatt
    16021 cttctacttt atgaagatca tgatggacga tcaaaatcgt ttttcctgaa ttcttcaatt
    16081 cctgcaagat gtcaaagatc tttcgttcac ttaacgcatc aatccctacg aatggttcat
    16141 ctaaaaagat ccattctgct ccttgggcta gagctcttgc aataaaaact ctctgaagct
    16201 gtccacccga tagttcgctg atctgtcttt tggcatattc ttccatccct acttttttca
    16261 aagcttgtct tgcacgttct ttttcttttt tcccaggtct ttgtccaatt cgtaaagatg
    16321 gatatgttcc taaaagtaca acgcctaata catcaattgg aaaggaaaga tccaattcac
    16381 ttcgctgttc cacgtaggct atttttttac ggactgagtt taccgactgt ccagaaaagg
    16441 tgactttacc agtagtagct ggtatcaact ctagcaaaga tttcataaat gttgattttc
    16501 ctgcaccgtt tggtccgata ataccggtaa ttttttgtgg atttatcgtc aagttgatag
    16561 ttttcaaaac agttttccct tgataagcgg ctgaaaggtc ttgaattaca atggccgtac
    16621 gttccaaatc ttacactcct ttttaggttt acctaacttt ttatttaaat atagtttaac
    16681 taaaatggaa agcattttca agtagaatga tgaaaaagtt tttttcgaat tttcctcgca
    16741 ttttatgaaa attttctgta gaatcacatc aagagttcaa taaggctaac ctaataatga
    16801 aaaacatgtg ttgaaacgtt tgattatttt ttcttgttga ctcaacaacg aaaggagttt
    16861 ttgtatggaa gaagaaaagt taactcgaag tcttagtgcc cgtcatatcc aaatgatcgc
    16921 actaggtgga acgatcggtg tcggtctgtt tatgggtgcc tcctcaacca tacgttggac
    16981 agggccctca gtgatgggag cctatgcgat tgcaggattc tttttgtatt tgattatgcg
    17041 tgctttaggc gaaatgcttt acgttgatcc ttcgactggt tcttttgcaa attacgcgag
    17101 cgagtacatc catcctgtgg ctggctactt gaccgcttgg agtaatattt ttcaatatat
    17161 tgtcgtagga ataagtgaag tgattgctgt aggatcttat atgaattatt ggtggcctga
    17221 tttgccggca attattcccg gaatcattgt cgtcttgttt cttatgctgg caaatcttat
    17281 ttctgtcaag gcgtttggcg aattagaatt ttggttttcg atcatcaaag tcataacgat
    17341 cattttgatg atcattgcag gtcttggggt aatcttattt ggatttggta atcatggtca
    17401 ggcagtaggg atttcaaatc tttggaaaaa tggtggattc ttcactggag gcgtcaaagg
    17461 atttttcttt gcattatcga tcgttgttgc ttcctatcaa ggaatcgaac tgatcggaat
    17521 gactgctggt gaagcagaga atcctaaaaa gacgatcatt gaagctgtcc agtccactat
    17581 cggtcgaatc ttgatttttt atattggtgc tatttttatc attgtgagta tctatccgtg
    17641 gaatcaattg agtgagattg gttcaccttt tgtacaaact ttttctaaag tcggcattac
    17701 atttgctgca ggactgatta attttgtcgt gattacagcg gcattatcag gttgcaattc
    17761 agggattttc agcgcaagtc gtatggtcta tacacttgcc ataaatggaa agatgtctaa
    17821 aaaatttctg aaactatctc gtcacggtgc gcctttttat cctgtcgtag caatctcagg
    17881 tggaatctta attggtctga tcttaaatta tcttctacca atgttcatca accagtcaga
    17941 aaatattttc gttttcgtat acagctcaag tattttgcct ggtatggttc cttgggtagt
    18001 gattttgatc agcgagattc gcttcaaaaa gaagcacgca gataaaatgc atgatcatcc
    18061 ttttaaaatg ccactatctc cttatacgaa ttatgtgaca cttgcttttt tagctatcgt
    18121 attgattttc atgtttatca atcctgaaac gagaatctca ttgattattg gactcgtttt
    18181 cttgctttac atgacgatcc attactttgt acgcgaaaaa agaaccggaa aataaaaaaa
    18241 gaacgaagct tcaccttagt ggaagcttcg ttctttttta ttgcttttat caattcaatt
    18301 ttagcgtctc accgggagtc aggaaataat tctccatgct catcccattc agcgacaaga
    18361 tcgtttctac tggtacgcca gttcgttcgg ctatttgatt tggaccttct ccttccaaaa
    18421 ctacggttgt cgcagcctgt gaagtcgcgc tgttggtatt agtttcagag gaatctgttg
    18481 tgtcttgtgt tgttcgagct tgcatttcag aagcttctac cgactctgct gtctcagaat
    18541 cagaagatgt tgtgtcttgt gtgcttgttg actctgcttg aagagaggcg ctagtctgca
    18601 aataaatatc ggaaatctta gcgacatgat tcgtataatt agccgataat tgattgatca
    18661 aagtatctaa gtctacaccg tctttatttt ctgctgcctg atacgctgca cgtaattttc
    18721 tgatcccttc gtcggagatt gcttgaagct cacgtgcttt ttggttagca actgctgata
    18781 aaccagctat tccctcttga tttccctgaa tttctgtctg gtattcttca atcaatgcag
    18841 gcgtcttttc ttcgatcttt ttcgaataat catttacgat tgcttgaagt tgctctggca
    18901 aaccatttgc tgttttttct tcagaagttt gtgtctcttt tgttttagtt gatgactcac
    18961 ttgtttgttt cgtctctgtt ttctttgtcg ttgtttccgt tatggtcgta cttttcgctt
    19021 ctgacgaatt ttttccatga aaatatagac ccgcaactcc tcctgctatg atcaaaaaca
    19081 cggcaactgc tgccccaatc atgatcatcc gcttacgagc tgcttgtttt tgctgtcttc
    19141 ttcgggtcat ccgaacattt tctccccact cattttcatc atacattttt ttcaccacca
    19201 atttttatgt ataatagcgt tatcaaacgc ttcatattct tattgtagca aataattgtc
    19261 gctgtgcaca tttttatctt tgttatttat aagaaaattt caccagttga gatgattcca
    19321 agattgtccg tcgatttctc cttgcagctg cattgttcgt tcgtctatct cagccatcag
    19381 tttctttatt tcatataaga gcgcacggtc ttgatattct gatttttcgc ttaattcatc
    19441 taattgttgg atcagccaat catttgtcat aatgaagttc ctttcatttc ttgcagtgta
    19501 aaggtcaagt cttcgatctg ctcttttgcc tcagtcagtg cttctacgat atatgcttgt
    19561 ttttcagatt cttcttcctg cattttctga ttttcctgta ctctttgaat aaaccagtca
    19621 atccgctgag tcagcgatgg tttgccttcc aatgagagat actgctgata agcaattagc
    19681 ttctcccaac gtttttcctg ggataaatac ataaattgtg ctaaaacgaa tggcggaaga
    19741 taggtcatat ggaatttatt tgcgattcgc tgatacggcc tcaaaaattc ggaaatcgta
    19801 tattcttctt ttcctccagc ttggtattct ttttccgcca cacctgttgt caatacgatt
    19861 cctagttctt tcccttttaa tacatgtgct gctcgttcca agacattatc ttgccaaatt
    19921 tttaaatgag caggagaact ataccagtaa aacggaaact ggaaaatgat ccgatcatgc
    19981 tgggccaaga gctgtagttc agtctctata tctatttttc cgtctggata acttgattcc
    20041 aaatgatgcc aagtagctgg taaaccagca gaagcttctt tgaaaaattg ctgatttaaa
    20101 gattcttcga tcgttggatg gctaatgatg ataagtgtac gcatcccttt tcctcctttt
    20161 tagtcttcgc tttacagtgt agcataaccg ccacaagttt cttttgccga tgcttgtcat
    20221 tttaaccaga ctaatcaggt tgtccaagaa actgcggttt gtttttccaa ctgttttttc
    20281 aaggtatcca aagcaatatt cttaaaattt tcgtttttca tacgtttacg atagttcgta
    20341 gccgtttctc cggtactctt tttgaacatt cggctaaata cgactagact attgaaccct
    20401 acttgatagg caatactttc cacggattca tccgtcagag aaagaagaaa cgcccctcga
    20461 ttgatccgta ccccgatcaa atattccttg atactggtac ccatatgatt tttgaatact
    20521 cgcgacaaat ggctccgatt gaccgctaaa tgctgggcga tttcctcaac agacaatttt
    20581 tcagcataat gtgcctcgat gtatttgact gtttcaacag ctaaagagga atgaaccttt
    20641 tgtacaccac cgctgacctt tccgccatct tttaaaagga ctgataaaaa acggtaggtt
    20701 aatgctgtta actgtaattc attttccaag gtttcttccg tatctttcat gcattctaaa
    20761 ataatgtcga tatatttttg tatgtcagaa gagaccatcg tgtattggtc ttctctgaaa
    20821 agcgactgcc gaatgatttc ttctgcagca ctcccaccaa atgaaagcca gcaatacatc
    20881 cacggatctt ctccatctga taagtaaaac gtcgagtctc ctggacgaat caagaacaga
    20941 tcaccttttt tcaattggaa ttgttgattt ttcacatgat aagatccttt gccttccatc
    21001 acgacatgca ggatgtaatc attgcgaata gcaggaccaa aagaatgaaa cggcatcgtt
    21061 ttagagaaac cacaaaaatc aaaatacaat tctttttcta cttgttgatg ttcctttttt
    21121 aaaaaaatcg attgctccac gtctatccct ccttactcct ttagtataat gcaacaaaaa
    21181 gttaaataaa agtcacagaa atatattaaa aaggaagcga tttcttgtta agtttaagtc
    21241 attaaaagat ctttgttatt cgttgtaacc ggtttactta gtattatata ttgaacagga
    21301 gaggaataca gattatgaaa agcaaaatga tccaacgtgt gtcctacgct ttaggcgctt
    21361 ttggacacga cacattctat gttacattaa gcacttattt tatggtattc gtgacaagcc
    21421 agatgttcgc aggaacagat aaggcaacga atgcaaaaat gattgctaca gttacaagct
    21481 tagttgtagg tatccgctta gttgaaatta tttttgatcc tttgattggt ggcatcatcg
    21541 ataatactcg tacaaaatac ggtaaattca aaccatggtt agttatcggc ggcgttgtta
    21601 gttccgtact cttggtagca atctttacca atttctttgg cttagctact cataatacga
    21661 ctttatttat catattattt gcaatcgttt tcatcttatt ggatagtttt tattcattta
    21721 aggatatcgc tttttggtca atgattccag ctttgagtac ggaaacaaag gaacgtgaaa
    21781 aatttgctac gtttgctcgt ttcggctctt ctctaggtgc aaatggtact actctagtcg
    21841 ttgtccctat cgttactttc tttacctatc ttgttactca taaacatatt gaaggcgctt
    21901 caggctggtt ctggttcggt gtgattgctg ctgttatttc aggcggtact gcgattatca
    21961 ctgccttggg cacaaaagaa gtcgatactg cgatccgtca gcaaaatgaa aaagctggga
    22021 tcaaagatgt gttcaaagca gttgctcaaa atgatcagtt gatgtggctt gcccttgctt
    22081 atatcgctta tgcgattgcc aatgtagcaa caactgccgt attattctac ttcttcaaat
    22141 atgtacttgg ccaaccaact gaattttgga tcgttggtct agttgctgcc ttacttggtt
    22201 tagtagctgt tccactattc ccaatgctta cacgcgtcat ctctcgtcgc tacgtttacc
    22261 ttggagggat cgcaatgatg atttcttctt actttttctt tacgatcgca ggttcaagtt
    22321 tgccaatcgt tatcatcggg ctagtattgt tctacttccc tcaacaattg attttcttat
    22381 ccgctttgat gacgatcaca gactctgttg aatatggtca atggaaaaat ggtatccgta
    22441 acgaagctgt tactctatca attcgccctt tactagataa aatcgctggt gctttgtcta
    22501 acgggatcgt agggtttgtc gctatcgctt ctgggatgac tggtaatgct actgctgctg
    22561 atatcactgc tcatggcgta acgactttca aagcttatgc attctatgct cctgctgctt
    22621 tgatgatttt ctccgctatt atttttgctt ggaagattac attgacagag aaaaaacatg
    22681 cacaaatcgt tgagaaattg gaaaacaaat tggctccagc tgatacaatg gaagatgaac
    22741 ttcacgatgc agtagttcct tcccattaag aaaggatctt gaaaatgaag attacagaaa
    22801 aagattttgg ccaaggttat cacttgataa ccttggagaa caaaaacaag ctagcacttt
    22861 ctatctctga tttaggtgct agaatcgtca gtttaaaatc aaatgatcga gaattagttt
    22921 tgggatttga caccgctgaa gaatatatcg aaaaagatcc gtatatcggt gcttctatcg
    22981 gtcgcaccgc agggagaatc gaaaatggcc acttttctct caatggaaaa acttaccaat
    23041 tagccacaga tccaaaaact ggacataatc tgcatggtgg tgctcctgga tttgaattaa
    23101 aaaaatggtc ttatgtcatt ttgaatggcg aaaatgaagc ttctgtcatt tttactacta
    23161 caagtcccga cggagaacat ggttttccag gtacaatgga tgtggaaatc cgctatactt
    23221 tgacaaaaga taatatctgg cgagtgacct ctcgcggcac cagtgaccaa gatactttat
    23281 tcaatccgac aaatcatgtt tacttcaact tgacgggaga tgcttctcag tcaatcgatc
    23341 aacatgagct ttggctcaat agcgaagctt atgctccttt gcgaacagat agtattccta
    23401 taggggtaaa agaaaatgct gcaggaagtg ctttcgattt tcaaatacct aaaaaattag
    23461 cgtccgtttt tgctagtgat ttggatcaaa aaaatcttgt tgacggaatc gatcatccat
    23521 ttttcttaaa agaaaccggc ttgggtaaag aagccgctcg cttgactagc cccgataaaa
    23581 gaatccaagt agatattgct acagatgctt ctagtgtggt tattttcact gcaaatttcg
    23641 gcacagaaac accagaaatg cgcaaccgaa aactggcaca tcacggtggc atcacatttg
    23701 agactcaaac agctccaggt gcagagcgct tctcagcatt tggttcgatt catttaaaag
    23761 ccggatcagt atttgaaaca gtgacagaat tcaaaatcaa aacaagaaag gaataactca
    23821 tcatggaaat caaaagaagc ttaaacacaa aatttgccga aacattcgga cctaaaaaaa
    23881 ctacacaata cttctcacca ggacgcatca atttgattgg tgaacacaca gattacaatg
    23941 gaggccatgt cttccccgct tccatcactt atggaacgca aggtgtagca gcacctcgag
    24001 acgataataa aatattagtt tattccacaa actttgacga tatcggtgtc attagtttta
    24061 ctttagatga gctagaatat gataaaaaag caaactgggc aaattacgta aaaggaatgg
    24121 tccttaaatt aaaagaagct ggacactcaa tcgatcatgg gtttgagtta ttagtggaag
    24181 gaacaatccc taatggtgca ggactttcca gtagcgcttc attagaatta ttagtaggtg
    24241 ttgtattaga agatctgttt gatttaaaaa cagatcgctt ggaactagtc caaacaggta
    24301 aaaaagtaga aaatgaattc atcggtgtca attcaggtat catggatcag tttgccatcg
    24361 gcttcggtga gctagatcat gcaatcttat tagatacaaa tacgctgaaa tacgaaatgg
    24421 ttccagtgaa actagatggt tatgcaatcg tcatcatgaa tacgaataag cggcgggaat
    24481 tggcagattc aaaatacaat gaacgccgta gcgaatgcga agaagccttg gcacgtttgc
    24541 aaaaaacttt gcctatctct tctttaggag acttagatga agaagaattt gaatcaacta
    24601 ttgaccaaat cggcgatgat acattgatac gcagagctcg ccatgccgtt tctgaaaatc
    24661 agcggacttt gaaggcaaaa gctgaattag aagctgggaa cttagaagca tttggtcaac
    24721 tgttaaatga ttctcaccac tctcttcgct atgattatga agtaactggg atcgaattag
    24781 atacactagt agatgctgct caaaaacaag aaggtgtctt aggtgcacga atgacaggag
    24841 ctggatttgg tggctgtgcg attgctcttg tcaaagaaga aaatatacct gccttcaaaa
    24901 acaatgtcta tgatgaatac atgaaagttg tcggctatgc tccggaattt tatgttgcac
    24961 acattggaaa tgggacaaca aagatcgatc cagaagctgt tcatgtttga taaaaaaatc
    25021 aacgatctca gctaaaaaca aatagataaa aataacctct cagcaatttc caaatgtgag
    25081 ccttttgctc gtatttgaaa attgctgttt tttattgaca cggaaagaga acgtgtgttc
    25141 taattgtgtt gaagaggtga ttgttatgtg ggcgaatcaa aatccggtat ttgtttatga
    25201 aaaagaacct tcaagagata ttttatgtat tgactgcaaa tctttttacg cctccgtaga
    25261 gtgcgttgaa aaggatcttg atccccttac aactaaatta gtagtcatgt cctatccgtc
    25321 cgattcgaca gaaacacggg gtagcggatt aatcttggct tccagtcctg cagcaaaaaa
    25381 agcttacggc atcaccaata tcagccgtgc cagagatctt cccttcccct atccaagtga
    25441 tttatatatt gttgccccaa ggatggccta ttatatggaa aagaacagag aaatcaattc
    25501 tatctataaa aaatatgcag acgaagcgaa ccatcatgtc tattccgtcg atgaaagctt
    25561 ccttgatgta acaaatagca tggcactctt tggtaaaaca aatgcgtacg acatggctaa
    25621 actgatccat aaagatgtgt ttgagcagac cggaatctac acgactatag gaattggcga
    25681 taacccgcta cttgctaaac tagccttaga caatggctca aaagacggaa agcaaatgtt
    25741 tgctgaatgg cgttatgaag atgtaccaga aaagttatgg aaaatctcag atatcactga
    25801 cttttgggga atcggcaaac gaacagcaaa acgtctgaaa caaatgggta tacattctgt
    25861 ttatgattta gctcacgcgg attattatca cttgaaaagc aagctagggg tcatcggtac
    25921 tcagctttat gcccatgctt ggggcatcga tcgaagtttt ttgggacaag aatatacacc
    25981 aaaatcaaaa agtatcggaa atagtcaaat cctagataga gattatacaa gacgagaaga
    26041 aatcgagatc gttatcaaag aaatggccga ccaagtcggc actcgcttga gacgatctgc
    26101 agcaaaaaca caagtagtca gcttatgggt aggatactct ttaggctatc ttgatccaaa
    26161 tggcaagacc gggtttcacc agcaaatgaa aatcgaagcg acaaatgcta gcagagatct
    26221 agcagatgct ttattgacca ttttccacca gcattaccac taccaggata ttcggcgtgt
    26281 aggcgtgaac tgttccgacc tcgtatacgc gacaggttta cagcttaacc tttttgaaga
    26341 tcctgaaaag cagataaatg aagcgaaagt tgattttgtt gtcgatacga ttagaaaaaa
    26401 atacggcttt caatcaatca tccatgcgca tagtctcttg caaggaggac gcgccatcgc
    26461 acgtagttct ctcgttggcg ggcatgcagg tggtatgagt ggattagaag gtggaaaaga
    26521 tgagacggac agcaaaaaaa ttcattgact ataacgagta tcgagatcgg ccatttggat
    26581 taaaatgggg gactgctttt gcgatggaag aactagtcaa cggtatcaaa aaaaatgaag
    26641 aagaagcacg caaacagcct cttcctaaac aacaaatgac attgcaagaa atcgatagta
    26701 tactgacaga agccttttta tctcgtaaag cagtaatcat tcagctcaat ttaaaagatg
    26761 aatttggaag actcttggat gaggtcgaag ggcacttcat tggtgaagcc tatgaagatt
    26821 actttatgat tgaccatcag ccgatattgt gggaagacgt acggtatatt gagttaaagg
    26881 aaacaaaaaa atggtttgtg acatgagagg ttgtgacaga agtgatcaac ttcaaaaaat
    26941 aagaaaggat ttttcgaaaa ttgcttctta aatttttgtg gaattttggc ttatttttcg
    27001 aagaagcaac ttctggaaca acgtatatta gatatcagag gttgtgaaaa agtcgcttag
    27061 ttttgagtaa caagccaagg cgctgatttc acaacctctg tctatctatc catctattcg
    27121 gtaagattca tgcatgacgt cttctttatt tagtatgtag agcccttttt cttctgacca
    27181 ttcaacataa atcagctgtt ttacatctgc atagccttct ttcttcaagt tctccataag
    27241 ccatgcttca gtcaattgat tggactgaag cactttatgc tgaatttgcc cttcatctac
    27301 aagcaggatc gataaagctt tgtcttcatc tgattttttc aatacactca tttgaccggc
    27361 attctctagg ataacatgtt tggcattttc aagaaaaaag cattcttgct gtcttaagat
    27421 tgctcgcaac tgctccatct cgatatggtt atttgtcaac tctgtcatgt tgatgacgcc
    27481 gtctttgatt agaacagatg gctcgccttt gacccaacga ttgacctttt catttttttg
    27541 gacgatacgt tcgataccat aaatcattac tgcccaaagc gcaatggcaa ataggacatg
    27601 ccctacatgg acattgtcat cataggttga ttcttctaag atcccaccta aaacaagcgt
    27661 atagactaaa tcaaacggtg tgatttctga catggatttc ttcccaagca aacgtaccac
    27721 taataataaa ccgatcaacc cgaacaccag tttcattgta attgaaaaaa tcattttttc
    27781 tccccctaac tatctgttaa ttaaattata tatgctttct cacgaacacg catggaatat
    27841 acaaggaaga aatgtatcct tttactaaaa aaaggactgt gacactgtag tgacccccaa
    27901 aagttagact tttttggagt ggagaaatcc actccttttt tttatgctct aaaatgatat
    27961 ttttacacaa ccgttacggt tgttttacga aattggacga gactttgcca gtttaatttc
    28021 tgttttatcc gttcattatt gtagtaatag atatagctat cgatggctga ttttaattcc
    28081 tcgaaacagt tataaacttt cccgtgaaaa atctcttgtt ttaataagcc aaaaaaattc
    28141 tccataagtg aattatctaa gcagttccct ttccgagaca tgctttgaaa gattttgtac
    28201 tccttcaatt tgtttctata agctttcatt tgatacgccc aaccttgatc tgagtgaaac
    28261 gtacgacgat agggacaatc gttagtggtc tggatagctt cctccaaacc ttccattacc
    28321 gctaatgcat tgggcttttc agagatacga taggataaaa tctcactatt gaacatatcc
    28381 ataaaaggat ctagatacaa tttcttttga cggataatgc ctttggcatc cacttcatgg
    28441 tatttaaatt cagttgtatc cgtcgtgatt ttctgatggc agatactggt gtagaaacga
    28501 cgatggatcc gatttttggc tactgctcca acttttcctc gataagaatt gtagcgacgt
    28561 gatttacgcg tgtatgccgt tacttttatt cctagcttct tgatcaagcg ctgaactttc
    28621 tttttattga tagagaaacc gcggtttctt agttcattgg tcatccgtaa acagccataa
    28681 tccttgtgct tctcccgaat tttaagcatt tccgcttcaa tctcttgatc tggattttta
    28741 cggtccaatc gcctttgcca tccaaataat catgaacgat ctttaattta agttcaaaag
    28801 tatatttaac cataaaaaat accgacctcc aaaagttaga ttttttggtc taacttttgg
    28861 gggtcggtac acaaatcgtg tcacagtccc ttttttgaaa ataaacggtg ttcaaaagct
    28921 tgcatctgcg gtaataagtc cagccaagtt aaaaacataa gaagctgttt ttacttggct
    28981 gttcttatta cttgatgcaa ggcagctttt tcacaacctc ttttcaaata tttcgttttc
    29041 ttattctaca aatggttctt ctcctaacgc ttgttgagcg gctagattca acatgctcca
    29101 ttgtcggtca aagcctggct ggaagaagaa gtcagcttct gctagttctt ctaaagtcag
    29161 tttgttttgg atcgctagtg ccaatacatt tccttgacct gtaacatcat aggtagataa
    29221 tactgctcct cctagcaatt gatgggtcat tggatccgag aataattgga tggacacttt
    29281 tggatttcct tgttcttctg gtacatattt tggtcgtaac gtatcttcat aataagacac
    29341 tcgtacatct actcctgctt ttgctgctgt aaagctattc aatccgcttg ctgcaaaatg
    29401 atagtcaaag acacttaatg cagaggaacc tacgactccg ccgaacggtt tgtttggtac
    29461 ttcttcaaaa agatgtttta ctacataacg tgcttctcta cgtgcaacag tcgctaaagc
    29521 gatcggcatt tttttgcgtg ctgggataga ataagcaagt gtagcatctc ctactgcata
    29581 cacatctgga agattcgttt gcaagtattc atttgtttgg atccagccac gttcatctaa
    29641 gtcgacgatc ccttttaacc attctgtatt tgccttcaca ccggcagctt gaatgactag
    29701 atctgctggg atttcttttt cacttgtttt gattgcctca acttttccgt taccagtaaa
    29761 agcttcgatt tttgcatttg tcaaaacttc aataccgttt tcatttaggt gttgttctaa
    29821 aatatctgtc atctcagcat ctaaataagt gcctaaaggt ctatcgatca catcaagcaa
    29881 tacgacttct ttcccagctt ttcggcttgc ttctgctgct tcgatcccaa tgtaacccgc
    29941 acctacgaca gcgatttttt taatagccgg atcatttaat ttttctttga ttttggttgc
    30001 ccaattgtat ccacgcatca agtagacatt ttctagatct gctcctggta ctggcagatt
    30061 gtttggtttg acaccagaac tcaaaatcaa tttgtcataa ggatattctt ttgtttcgtt
    30121 atctgatgcg ctagtaaccg ttactgtttt tttatcggca tcgatcgttt ttacttcgtg
    30181 attatttaaa atatgaacat tggattgcgg aaagttttct ggtctgaaat tacgtacatc
    30241 atttacatct gttaccttgt cttctaaata cagctccata ccacatgaca tgaaggaaat
    30301 atagtctcct gcttcaaata gcgtgatatc tacattttca ccgtatcgtg tcaatagttc
    30361 caagatcgat tgatgccctc catgagatgc acctacaatg acaactttga ttttttccat
    30421 tgatgaaacc tccttgaata ttcttttaaa atcattctaa acaatttaaa cttaacatta
    30481 cattagttat gaatcaaaag aaacgcgttc atttgaacta aatttaaaaa acaatcgtct
    30541 ccttgcagaa tataccgcaa aattaacgat tgtttttttg ataaaatcta tgaatcactg
    30601 ttctgttaaa aagtctttga tccgtttgca ggcctcatgt aatttttcca tgctgctagc
    30661 ataagaaatc cggatataac cttctccttc tgggccaaat gcaattcctg ggatacacgc
    30721 aacttttgcc tgttccgcta attctacaca aaactgccaa gagtcagttt gaatggcagc
    30781 tgggagtttg caaaacaagt aaaatgcacc gtttggctgg gtcacttcaa atcctaaagg
    30841 cgccaattgc tggaccagat agtctcttcg tgtttgatat tgctctttca tattctcgct
    30901 atctttcacc ccatttatta gcgcttcagc cgcagctttt tgcgacatcg ttgtagcggc
    30961 agtcaccaga tattgatgga ctttaattag ttcgtcaata agggcttttt gagcaaaaat
    31021 aaagcccact cgccaaccag tcatcgcatg tgacttggat aacccgctta tcacgattgt
    31081 ttgttctgga agataagtag ctatggacat atgcgttcca ctataagtca attcagcata
    31141 tacttcatca cttataacaa ataccggata tttttttaaa acctcagcga ttgctttcaa
    31201 ttgttttgca gacaatgtcg tcccagtagg attgttagga tagtttaaaa tgactgcttt
    31261 gacagctgat ccatattgtt caaatgctcg ttcaaattgt tccggctgac agacaaaccc
    31321 tgtgtcagaa gtatctatcg ggatcaattc agcaccagct agttccacta atggctgata
    31381 tcctgaataa gcaggcgctg gaataagaac cttgtctcct tcttccaaaa cagataacaa
    31441 cgcagaggca atcgcttccg ttgcaccaac tgtcgtcaaa acttccgttt gcggatcata
    31501 agttaatcca taacgctgtt gctgaaatat acaagccgct tcccgcagtt cctctaatcc
    31561 tctcattccg gtatagtgag aaaagtcttc ttcaatcgct gctattcctg cttgcttgac
    31621 atgttcagga gttggaaaat ccggttctcc cagggtgagt ttgatcacat ctggtatcga
    31681 cgatatttgc tgatcgaatt gtcgtatttt tgatacttct attctagaaa gcctaggact
    31741 aaatcgattg gataaattca tcattattta ctcgctcctc ttttgaactg ctatcgattt
    31801 ttacaagaag attttctgta aaaagaatcg ttttgtacca ttctacacca ttttctaaaa
    31861 catccatatc aaaatcgaac gtcggatcat gtaaagcagg ggtatcaccg acacctaaaa
    31921 agaaaaatac acatggatat tgttgcgtat atatgccaaa atcttcggct tgaagaactg
    31981 gagcatctaa ttcatgtagt ttgtttgaat ctaacagtgc tgcatataag cctctatggt
    32041 tgattacagc tggatagccg tcattatagc gaaaagaaat agtagctgta gagtgaaact
    32101 gttctgctaa atggtctaat cccctcttca aataattttg catatctgaa ctgtaagaac
    32161 ggatacttcc ggatacaata acttctccag ctaaaacatt tcggatcgtt ccaccttgga
    32221 tttggccaaa tttcattaga tgattccact ctgccggtaa ttgccggtca aaatcataaa
    32281 cattcttcaa aaatcgtaca gctgcctcca gactatcgat tcctttttca ctagaagcaa
    32341 tatgtgcaga ctggccttta atgataatgt ctgtttcact gctttgtgcc atcaaaggtc
    32401 ctggtcgaga gaaaacttct cccttagcta gtccaggcca tagatgaaag ccaaatattg
    32461 cttgtacttc atatttatcc aacaatcctg actgaataac gctttgtgcg ccaccggaaa
    32521 tttcttctga tggctgatag atcagtacta cattataaat aagatcctca atgtgtttag
    32581 ttataaagtc agtcaaccct aaaagcatcg ccatatgtcc gtcatgacca catgcatgca
    32641 tataaccttc atgcttagaa gcaaaagaga gattcgtttc ttctttgact ggcagtgcat
    32701 cgatatctgt tcgaaaagca atcgtttctt cttgctgatt atcaaaataa aggagtagtc
    32761 ctgtttcgcc gatttcatgg ataacggcct cgctgttttt tacttgttca tataaatatt
    32821 tttttgtctt gaattcatga aacccgattt caggaatttg atggagtgcc attcgatatt
    32881 ctttcagcat ccgtattcac catcccttca tagataccct tcactactgt ttcatattga
    32941 ttattcggta caccgatgat cagattcaac tcctgtgctc cttgtgataa gatcgatgtg
    33001 cgaatttcta atttgttcaa tatcgataac actttcccag ataatccaat caattcttta
    33061 tgcggacccc caacgacaga gatcaaagct aagtcttcga tcacgacgat ttcttccacg
    33121 ccaagtgttt cttttaattc tttcgtgatg agaaataact tatcggcaat cgcttctgct
    33181 gagacgatca ctccgatatt gtcgatccct gaaggaatat gttcgattga tatctcatgt
    33241 ttagcaaaaa tgctcattgc tttccagata aacccgattt catcggacat atgacgtttg
    33301 aataaagtga tggaaagaaa atccttgcgt ccagctattc ctgtcagtcc attttccttt
    33361 tctatttcgt gattcgatat ttttgtccct tgagcgaaag gatcattggt attttttatt
    33421 tggatgggga tatcagcctc ttgaactggg aaaacagctg cttcatgaag aacgttagcc
    33481 cccatataag ccatctctct cagttctcta aaagagattt cctcgatgat tttaggatga
    33541 tcgatgattc gcggatcagc catcatgatt cctgaaacat ccgtccaatt ctcataactt
    33601 tccgcttgca acatcttcgc cagtatcgcg ccggtaatgt cgcttccccc acggcccaat
    33661 aatttttctt ttcctgcagg gttcgccccg taaaaccctg gaacaaccat tttcggatat
    33721 cttttaaatg cttttgtgat ttcctgtgct gttttttcct gatcgatttt cccatcatag
    33781 tcaaagaaaa tcaggtcttt tgcgtcaata aactggtaac caagatactc agccatcagt
    33841 tgagcagtca gatattcacc tctacttact aaataatctt cagttatctt ttcgtcttcc
    33901 agttgtttct gtatgcgtgc tagttcatct tcaataggta actgcagggc aagaacatct
    33961 cttacttgaa tgaaacgatc acaaatcctt ttccaaaggg gtgtccaatc aacatgatgc
    34021 tgaaggtaag cataaatcaa ataaagtaag tctgttactt tgtcatcatc tgacgtacgt
    34081 ttgcctaaag cagaaacaac gatcacttgg cgattgggat ctgaaagaac aattgtacgg
    34141 acttttttga attgctcttc atgagacatc gatgttccgc cgaatttcgc tactttcagc
    34201 gtattactca tcttgccacc tccgctattt tctgcattaa agctgcagca atctgtacgg
    34261 cattcgcagc ggctcctttg cgtatgttat cggcagtcgt atagaacaac aatccgttct
    34321 ctaaagaagt gtcttttctg atcctcccaa cataaaccat atctgttcct ctagcaagaa
    34381 tgctcgtcgg atatttatgt gctgctagat catcttctaa ttgaatccct ggaaactggc
    34441 tgagacactc tctaatttct tccgtcgtaa actctttttg cagttgaact gccacagaga
    34501 ctccatgtcc attttcgatt ggcacacgta cgcaggttgc tgatactggc aaagcaggct
    34561 gatgcagaat cttacgggtc tcatttatca ttttctgttc ttcttttgta tacccatttt
    34621 ccaaaggctg atcgatttcc ggaatcaccg tttggctaat attataagga tacaaaactg
    34681 gttcctcgtc attttgcgta cgatgaagat cttcaatgcc tttttgcccg gaaccagaaa
    34741 ctgcttgata ggtcgaataa ttgactcgct ggataccaaa ggcttcttgt aaagctttaa
    34801 gcggaagtac cgcttgaatg gttgaacaat ttggatttgc aatcagccga ttcaatgtat
    34861 agtcttccag attgacctct ggaacgatta atggaatatc cgaatcttcc cgccatgctg
    34921 aggaattatc tatgacaaat agtccctgac ttcgtgcgat tggcgcaaac tctttggata
    34981 cagaagcacc tgcagaaaat agcgctaaat catatccttc aaatgaagtt tctgtcaatt
    35041 cctgtacagt gtgtttcttt ttcttgaata cacaaacttt accggctgaa cgttttgaag
    35101 ctaataattt caatcgtcct actggaaaat catattcttc taacactttg agcatcgttt
    35161 ggccgactaa tccggttgcc cccacaatac aggtatctat ttcgcgcatt tttctccatc
    35221 tcctctataa ttcgcgtaac gcagcaatca atgctgtatt ttcttttgtc tgatcatcac
    35281 ttatttttaa tacacgtgca ggtattccac cgacaacagt ttctgctgcc acatcttcta
    35341 aaaccactgc tcctgctgcc acgactgcat cttttccgat atggacacct tcaacaatca
    35401 ctgcattcgc acctaccagt acgccgtctt ctacgataac cggtttggcc gatgctggtt
    35461 cgatcacacc tgctaagaca gctcctgcac caatatggca gtttttgccc actgtcgctc
    35521 gtccacctaa gacagcaccc atatcaatca tcgtgttttc accgattact gcaccaatat
    35581 tgataatcgc tcccatcatg atcacggcat tgttgccgat tgacacttga tctctgataa
    35641 tagcacctgg ttcgatacga gcattaattt ttttaatatc tagcaaagga actgcgctgt
    35701 atcttgcagc agtttcgatt tcgatttcaa tgattttttc tgattcagct tctaaaaccg
    35761 gagcaatcgt ctcatagtca ccaaaaacaa cgaagctgct tccttcacca aaaactttac
    35821 tttctttaaa ttcgattggc tcggttacat tcagatagac tttcacaggc gtctttttag
    35881 atgccgtttg aatgtagcgg atgatttctt gagcttccac gttttttaca cccctttgtc
    35941 cattaataaa tcttctaatt tgtaataacc tggtgactga tctttcaacc agtccgctgc
    36001 ttctactgcg cctgcagcaa agattcgatt agaaaaagat tcatgtttga tcgtaaatat
    36061 ttcatcagta gcagcaaaca atacatcatg ttcaccaggt aaagaacctg cacgaatact
    36121 gtgtacacca atttggtttt cttctctttt tttcccttgc cagttgtata cgggctgcag
    36181 cttttgcact tcttggattg tttgaagcag catatttgca gtaccagatg gtgcatcttt
    36241 tttacgacta tgatgttttt caatcagttc gatctgccag ccttgcaaca ttgcagctgc
    36301 ttgcttgatt agttgattca tcaccatgat gccgaacgaa aaattagcac tatacatcac
    36361 aggtacatgt ttggcatgtt cttttatttc ttggaactgt gattgtgtat aacctgtcgt
    36421 gccaatgact aacggaagtt gatatgataa gctatacgaa aggattttct ctaaattatc
    36481 tggatgagaa aaatcaataa gcagttctgg cagttccgta aaagcaagcg tagttgcttc
    36541 tttaggtgcc gtccgtaaaa gaaggaccgt atctcctctt tcttgtgcaa cttcttcgac
    36601 tctatttccc atctgtccac tgccaatcag tccgattttc atttttcaac cgtcctttct
    36661 tttaacaaaa gctgagctaa ttgtttcttt ttctcttttg aaagagaaac taaaggcagg
    36721 cgtagaggtc cggaaggtaa gtctaataat gacatggctg ctttgacagg aataggattg
    36781 gtctcactga acaaggcatg gatcagttca agtgcattta attgcttttc ttttgcttgc
    36841 tgccatcttc cgtcctgcgt atcttttact aattcatgga ctattttagg ttggatattc
    36901 gcccatacac ttattacacc gcttcctcct acactcatta ctggtaggat caaatcatca
    36961 tttccactgt aaaggaagaa ttcttctcca atcaaccgag cggcatccat cacataagcc
    37021 atatctcctg aggcttcttt caaaccaatg atattaggat gttctgctaa atttactaat
    37081 acatggattg gaatcgacat gcctgttcta gaaggaacat tatacaaaat aagcggaatc
    37141 ggtgaggctt ctgcaattgc tgtaaaatga gcagctagtc ctgcatcact tgttttgttg
    37201 tagtaagggg tgatgaccaa aagtgcgtct gcccccaaag aagcaaaatg cttcgctttt
    37261 tcaatcgttt ctttcgtatt attcgttcca gctcctgcaa tcaccggcac acggccagcc
    37321 actttcttta ctgtcagttg aaggatctgc tcctcctcag attgtgtgag cgtagaagat
    37381 tctcctgttg tacctaaaac taataatcca tctgtttgtt catctaaatg gaattggatc
    37441 aatttttcca aaccagcata atctactgat ccctcttcat tcatcggcgt gatcagcgcc
    37501 acgatcgaac cttttatcat cttcattcct ccaatattct tgtccattca tgaactgctg
    37561 ggccagccat ttgtatcgca tcctcaccag agatcatcaa gtctccttgt tccaagtgaa
    37621 caactacctg atctgctgaa attttcccag tttccttagc aaccacataa gaggcacagc
    37681 aacctgtacc gcaagcaagt gtccaaccta ctcctctttc ataggtacgg acgatcaatt
    37741 cttgttcatt gactacttct acaaagttga cattggtttt ttctttgaac aaaggatgat
    37801 gacagatcag ttcacctctt tttctccaga cttcctcacg cgcattttca acaaaaacaa
    37861 cggtatgaat cgttcctaaa aataaactag tgatcgttac tttttcacca ttgatctcta
    37921 tttcctggtt ttttattgga taagtttgat tagctttgat tttttccgga gaatcatctg
    37981 gcactcccat atctacgctg caataaaaag gtgagggatc caacaccgta atttttcttg
    38041 tgcctgctaa tgtctctacg ttgaattcct gttcattcat catgttctga tcatttacgt
    38101 atcgagcaaa acagcgaatg ccatttccgc acatcggcgc gcggctgcca tctttgttgt
    38161 aatagatcat ttccaacgga tcgattttta cggcgatcaa tccgtctata tcatattttt
    38221 cttttgtgca taaagaagcg gctaattgac tataatctgt gtcttctgcg taatctaata
    38281 agataaaatc atttccgcag ccattatatt tatgaaggat cataggaaac ttcccccctt
    38341 agtagatcag aaaatgcttg tctgcgaaca atcagtcttg attggccatt attgacaaaa
    38401 acaactgctg gtcgtgtcgc tcgattgtag ttgctgctca tcgagtatcc ataagctccc
    38461 gtggaataaa cagctaaaag atcacccgat tctgctttcg gcaaataagc ttctttaatt
    38521 accacatcgc ctgattcaca catttttcct gcaatcgtca ctttctctgt tttttcttcc
    38581 gacagttttg ctgctaaatc acaatcgtat tctgcctgat acagagctgg acggatatta
    38641 tctgtcatcc cgccatctac aaagtaatat tttttatgtg gtgtcttttt gatgaaacca
    38701 atcgtgtata gtgttgtacc agcttctcca acaatacttc tacccggttc gatcagtaat
    38761 tgacgaatgg tcagttttcg tttttccaaa gcttcttccg tatattcaat cagttttttt
    38821 agtgcctctt cgactgggat cggctgatca ttttgtgtat aacgaatccc aaaccctccg
    38881 ccaagattca aggaaagagg ttggtcaaaa tcggttaagt aattcgtcaa tttgtcgatt
    38941 tcagctaacc aagcagtaac atcaaaaatt tgtgacccga tatgagcatg aaatccttct
    39001 aactctagat agctgctttc ttttatcaac tgaagggctt gttggcactc tttactttca
    39061 tagctcatgc cgaattttga atcaatatgc gcagtgacga tatattcatg agtatgtgct
    39121 tctactccga cattcaaccg aagcatgata caaatttttt ttaaatagcg ttggcttaat
    39181 tctgctaata attcaacttc catcaaatta tcggcaacaa tatgcaacaa atcattttca
    39241 agtgcataga tcagttcctc tggcgtcttg ttgttaccat ggaagtagat agtatctcta
    39301 ggaaaatcag cttgcaaagc ggtatagatt tccccaccac tgacaacatc tagcccgaat
    39361 ccatattcac gaactaaatg cagcatctca atcgtttgga atgctttaga ggcgtaaagc
    39421 acttttgtat caaaacgttt cgatacaaag cctcgcttga atgacagcat cgtttgtctc
    39481 atcttttctt catcatacac atacagaggt gtcttatact tttctgccaa tgtgcttgct
    39541 gtgactccgc caattatcaa ttctgtcatt ttttcattcc tcttttcact ttttttccgt
    39601 ttttattgga atttcatttg ataaagattt gaattttatt ggaataatat taaacaaaat
    39661 tttctgaatt gtctagtata tttcgaaata tttttttaaa aataagaata aaaatggtta
    39721 taaaaacaaa atacccgaac tattagaagc tatttataca taaaaacata aattcaacac
    39781 tatttagtga tgaaaaaata ttttcgcttc aatatagttc ggaatagttc ggatattttc
    39841 aattttatga atcatttcaa tttcttcata aataagtgca gttttttctt tctctagctt
    39901 ttgccattca tctttcaaaa tataaataaa atcaaatact tttttatttt tcaatttgct
    39961 gattccacaa aaaacctctt gaaaggcaga tccgatgatc tgcctttcaa gaggtcaagc
    40021 tctgttttca aattcttatc aatccgttag cgaattattt tgacgcacgt tttacataag
    40081 aaccttctgc tgtattgatt tccaacaatt catctgtttc gatgaagtca ggaacattga
    40141 ctactaagcc agtttccatt gtcgctggct tacctgaacc agtgactgtt gcacctttga
    40201 tggaaggttg tgtttccaca acacgtaata cgacagttgt tggtaattga acgccgatga
    40261 cttcgctacc gaaaaattgg atttttactt ccatgttttc taggatatat ttcatttctt
    40321 cttctactgt ttctacagga atttcatatt gctcatatgt ttccaaatcc atgaagaaag
    40381 ctgtatcatc catcgtgtac aaatattgta caggttttgt gtcgatatgt gctttttcaa
    40441 atttttcttc tggtctgaac gttgtgtcgt atgttgcacc tgaacggaca tctttcagtt
    40501 tcatacgcat aactgtattt cctttaccag gtttatggtg gcttgcatcc agaactttaa
    40561 tcagtttgcc gtcttgtacg aaagtcatac cggctcttaa atcacttgct gagatcatta
    40621 tttttgctcc ttcttttctt tatcataatc aactttattg tatcagacaa ccggccagaa
    40681 aactagagta ttctctatct tttaaggtct catgcctatt tgtcagaaaa ataaacacgg
    40741 gtaaacgatg ctatcgaaat atttttttct ttcaagcctt tgacttttat tttttttcat
    40801 gctatcgtta ataaaaattt tacttggagg gatacacatg acagtcagca accaaaaaca
    40861 attgaatcgt tgctgttgta tggtgcataa aagcagcata ctatttgtca tgtctattaa
    40921 ttgcccttca tagaatgagt accaggtaac ttatcttttt atctactcaa tttttctcta
    40981 tgtctttcaa ggagctgatt taatagtcag ctcctttttt tattatcttt ttttaggagg
    41041 cccacatgat tatctcgaca attacagaag ctattggttc cacaccgctt tatcgctttg
    41101 acaatcagca ctattctatg cctaaaggtt ctgctattta tgcaaaacta gaatatttaa
    41161 atcctggagg aagtatcaaa gatagattgg gcagttattt gatcaaagaa ggatttgccc
    41221 aaggaaaaat caatgaaaaa acgacgatca tcgaaccaac tgccggaaat acaggaattg
    41281 ggatcgctct ggccgcactc acatacaaat taagaacggt ttttgttgtt ccagagaagt
    41341 ttagcttaga aaaacaacaa ttgatgaaag cattgggagc aaagatcatc cattctccta
    41401 gtgagcaagg aatcatcggt gccatcagca aaagtaaaaa acttgcagag gagatatcaa
    41461 acagctatct gcctcttcaa tttgaaaata aagataatcc tgctgcttac tatcatacgt
    41521 tagggccaga aatctttcat gaaatcaaag aaaatatcca tagttttgta gcaggaattg
    41581 ggagtggtgg aacctttgct ggaacttcta cctttttgaa agaaaaatat cccgacattc
    41641 gaataatcgg tgtagaacca gagggctctg tgttaaatgg tggtgatcca gcccctcacg
    41701 aaattgaggg tatcggtgta gaattcattc cgccgtttct ttctcccctt tcaatcaatc
    41761 agatcgagac aatctctgat gtagaagggt ttaactatac taggcagtta gcacgagaac
    41821 aaggactatt agttggaagt tcgagtggtg cggcttttgc agcagcatta agagaaattc
    41881 gtcgtttacc acctggtcat cgtgtcgtta cgatttttcc agacgcagca gaccgctatt
    41941 tatcaaaaaa catttactta taggaggaaa gaaacatgca cattcaaaca aaattgatcc
    42001 acggaggcat cagtgaagat cccacaacag gagctgtcag tgtacctatt tatcaaacat
    42061 ccacttatcg gcaagatggc gtcggccagc ctaaacaata tgaatattca agatctggta
    42121 atcctactcg ttttgcattg gaagaactga ttgctgattt ggaaggcggt gttcgaggat
    42181 tcgctttttc ttctggtctt tctgggattc atgccgtttt ttctcttttt caagctggcg
    42241 atcatatctt attaggagat gatgtctatg gaggaacatt ccgtctattt gacaaagttc
    42301 ttactaaaaa tggtctcgag tacacgatca tagatacgag taatcttgat aaaatagaac
    42361 aatccatcaa acccaataca aaagcacttt atcttgaaac acctagcaat ccgcttctaa
    42421 agattactga tttagaaaaa agtgccactc tggctcatca acatggattg atagttattg
    42481 ctgacaatac atttgctact ccttattttc aaagaccttt agatctcggt tctgatatcg
    42541 ttgtccatag cggtaccaaa tatctaggcg gacacagcga tgtagtagct ggtttagtga
    42601 cttctaacca taaagatcta gccgatcaga tcggatttta ccaaaatgct atcggagccg
    42661 tacttggtcc ccaagatagc tggttacttc agcgcggaat caagacacta agtgttcgta
    42721 tggaggaaca tcaaaaaaat gcttttgtgg tagctgattt tctattttct catccagcag
    42781 tagaaaaggt ctactatcca ggattacccg atcatgaact ccatggagtt gctaagcagc
    42841 agatgagcgg ctttagcgga atgatttctt ttactttaaa aaatgaagaa tctgctattc
    42901 catttgttga atcactccaa ctatttactt tagcagaaag tttaggtggc gtagaaagtt
    42961 tagtggaaat tccttctgtc atgactcatg cgtctattcc aaaagaaaaa cgagaagagg
    43021 ctggcataaa ggacggcttg atccgtcttt ctgtaggaat tgagtatggt caggatctca
    43081 taaacgattt agctcaggca tttgacagaa tcaagaacta gaagtcccaa taaacgactt
    43141 agcagaaaaa tctaaaaaat atttcagatt gctacacctt ggaaacgata agtgttagaa
    43201 tcaagtagaa agacgttttt gtaaatttca ctaaataatt ctttagtgaa aagcatcaaa
    43261 acgaatgaac aaacagatag gagtcttaca aatgagaatg aatgtcgaaa gttttaatct
    43321 tgaccataca aaagtcaaag caccttatgt acgtgtagct gatcgaaaaa cgggtgcgca
    43381 tggcgatgtc atcattaaat acgacgtccg ctttaaacaa ccaaataaag aacacatgga
    43441 catgccaagt ctacattcac ttgagcactt gactgccgaa ctaatccgca atcacgcaga
    43501 ttacatcgta gactggtctc caatgggatg ccaaacaggc ttttacttga ctgtattgaa
    43561 tcatgaaagc tacgaagaca ttttagatgt tcttgaagca acaatgaaag atgttcttga
    43621 agctactgaa gtacctgcaa gcaatgaaac acaatgtggc tgggctgcca gccatacact
    43681 agaaggtgca caagctttgg ctcgtgaatt tttagcaaaa cgcgatgaat ggtctgaagt
    43741 agaagcatag tttctaaaag aacgtaaaga tgagaagcaa tatagctatc ttatcttcgt
    43801 attttattaa atatttattg tctgaaaaga gttctaaacg ggaaaacaaa tagctagttt
    43861 tcagtttagc gctcttttta ttatataaaa tagaaaggag aaaactttat gtctatttat
    43921 aattttgaaa ctactttaga agacggcact gtctattcat tgaaaaaata tgcaggcaaa
    43981 ccgataatca ttgttaacac tgcaacaaaa tgcggtctag cacctcaatt taaacaatta
    44041 gaagaacttt accaagctta tcagacacaa ggtctggtta tcttaggctt tccttccaat
    44101 caatttaaac aagagctaga ttcagcgact gaagcagcag aagcttgtaa aaccacttat
    44161 ggcgttacct tccctatgca tcaattgatt gacgtaaatg gagaaaatac agatcctctt
    44221 tttacttatc ttaaaaatga agctcctggt actgtcacta aagcaataaa atggaatttt
    44281 actaaatttt tgattgatag aaatggcaaa gtaatcgaac gatttgcacc acaaacttct
    44341 ccattgaaaa tgaccgctgc aattgaatcc gttctttaac ttaataaaaa taaaaaaaga
    44401 tgaactattt ctagttcatc tttttgcctt tgcgtggcga cgtcctactc tcacaagggg
    44461 caacccctca ctacaatcgg cgctaagaag cttaacttct gtgttcggca tggttacagg
    44521 tgtatccttc tcgctatcgc caccacactg tggtgttatc ttttattgag taaattttgt
    44581 tcactcaaaa ctggatttga agtca
//
LOCUS       DACGLW010000013        43679 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.013, whole genome shotgun sequence.
ACCESSION   DACGLW010000013 DACGLW010000000
VERSION     DACGLW010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43679
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.013"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            complement(105..629)
                     /locus_tag="IXT53_000794"
     CDS             complement(105..629)
                     /locus_tag="IXT53_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ4904642.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="IXT53_000795"
     CDS             complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="IXT53_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HAQ4904643.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1218..2198)
                     /gene="pta"
                     /locus_tag="IXT53_000796"
     CDS             complement(1218..2198)
                     /gene="pta"
                     /locus_tag="IXT53_000796"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HAQ4904644.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2351..3031)
                     /locus_tag="IXT53_000797"
     CDS             complement(2351..3031)
                     /locus_tag="IXT53_000797"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HAQ4904645.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3157..4026
                     /locus_tag="IXT53_000798"
     CDS             3157..4026
                     /locus_tag="IXT53_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ4904646.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4052..4567
                     /locus_tag="IXT53_000799"
     CDS             4052..4567
                     /locus_tag="IXT53_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4904647.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4614..5177)
                     /locus_tag="IXT53_000800"
     CDS             complement(4614..5177)
                     /locus_tag="IXT53_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ4904648.1"
                     /translation="MIRFARKEDAPEIAPLILVILKDMELPFLKQTGEQKTLEILEEA
                     ITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKYQLDASTRLFI
                     DKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLSVDEKNPKAKK
                     LYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5232..5411)
                     /locus_tag="IXT53_000801"
     CDS             complement(5232..5411)
                     /locus_tag="IXT53_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4904649.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5488..5997)
                     /locus_tag="IXT53_000802"
     CDS             complement(5488..5997)
                     /locus_tag="IXT53_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4904650.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6093..6473)
                     /locus_tag="IXT53_000803"
     CDS             complement(6093..6473)
                     /locus_tag="IXT53_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ4904651.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6803..7552
                     /locus_tag="IXT53_000804"
     CDS             6803..7552
                     /locus_tag="IXT53_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HAQ4904652.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(7736..9013)
                     /locus_tag="IXT53_000805"
     CDS             complement(7736..9013)
                     /locus_tag="IXT53_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4904653.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(9092..9736)
                     /gene="pcp"
                     /locus_tag="IXT53_000806"
     CDS             complement(9092..9736)
                     /gene="pcp"
                     /locus_tag="IXT53_000806"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAQ4904654.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(9935..10879)
                     /locus_tag="IXT53_000807"
     CDS             complement(9935..10879)
                     /locus_tag="IXT53_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAQ4904655.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(10881..11567)
                     /locus_tag="IXT53_000808"
     CDS             complement(10881..11567)
                     /locus_tag="IXT53_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAQ4904656.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            12041..12361
                     /locus_tag="IXT53_000809"
     CDS             12041..12361
                     /locus_tag="IXT53_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAQ4904657.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            12515..13420
                     /locus_tag="IXT53_000810"
     CDS             12515..13420
                     /locus_tag="IXT53_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ4904658.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            13417..14190
                     /locus_tag="IXT53_000811"
     CDS             13417..14190
                     /locus_tag="IXT53_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ4904659.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(14371..15795)
                     /locus_tag="IXT53_000812"
     CDS             complement(14371..15795)
                     /locus_tag="IXT53_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ4904660.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            16006..16452
                     /gene="nrdI"
                     /locus_tag="IXT53_000813"
     CDS             16006..16452
                     /gene="nrdI"
                     /locus_tag="IXT53_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAQ4904661.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(16517..18499)
                     /locus_tag="IXT53_000814"
     CDS             complement(16517..18499)
                     /locus_tag="IXT53_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ4904662.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(18513..19409)
                     /gene="murQ"
                     /locus_tag="IXT53_000815"
     CDS             complement(18513..19409)
                     /gene="murQ"
                     /locus_tag="IXT53_000815"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAQ4904663.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(19412..20473)
                     /locus_tag="IXT53_000816"
     CDS             complement(19412..20473)
                     /locus_tag="IXT53_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ4904664.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(20684..21211)
                     /locus_tag="IXT53_000817"
     CDS             complement(20684..21211)
                     /locus_tag="IXT53_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ4904665.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            complement(21278..22780)
                     /locus_tag="IXT53_000818"
     CDS             complement(21278..22780)
                     /locus_tag="IXT53_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAQ4904666.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(23038..24159)
                     /locus_tag="IXT53_000819"
     CDS             complement(23038..24159)
                     /locus_tag="IXT53_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ4904667.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            24285..25226
                     /locus_tag="IXT53_000820"
     CDS             24285..25226
                     /locus_tag="IXT53_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAQ4904668.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(25376..26272)
                     /locus_tag="IXT53_000821"
     CDS             complement(25376..26272)
                     /locus_tag="IXT53_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAQ4904669.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            26541..27881
                     /locus_tag="IXT53_000822"
     CDS             26541..27881
                     /locus_tag="IXT53_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ4904670.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            28099..28542
                     /locus_tag="IXT53_000823"
     CDS             28099..28542
                     /locus_tag="IXT53_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAQ4904671.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(29046..30464)
                     /gene="nhaC"
                     /locus_tag="IXT53_000824"
     CDS             complement(29046..30464)
                     /gene="nhaC"
                     /locus_tag="IXT53_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HAQ4904672.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(30867..32090)
                     /locus_tag="IXT53_000825"
     CDS             complement(30867..32090)
                     /locus_tag="IXT53_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HAQ4904673.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(32121..32960)
                     /locus_tag="IXT53_000826"
     CDS             complement(32121..32960)
                     /locus_tag="IXT53_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HAQ4904674.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(32977..33657)
                     /locus_tag="IXT53_000827"
     CDS             complement(32977..33657)
                     /locus_tag="IXT53_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ4904675.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(33659..34732)
                     /locus_tag="IXT53_000828"
     CDS             complement(33659..34732)
                     /locus_tag="IXT53_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ4904676.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     gene            complement(35379..36050)
                     /locus_tag="IXT53_000829"
     CDS             complement(35379..36050)
                     /locus_tag="IXT53_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ4904677.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(36137..38815)
                     /gene="mgtA"
                     /locus_tag="IXT53_000830"
     CDS             complement(36137..38815)
                     /gene="mgtA"
                     /locus_tag="IXT53_000830"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HAQ4904678.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            39474..39911
                     /gene="ndk"
                     /locus_tag="IXT53_000831"
     CDS             39474..39911
                     /gene="ndk"
                     /locus_tag="IXT53_000831"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HAQ4904679.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(39933..40799)
                     /locus_tag="IXT53_000832"
     CDS             complement(39933..40799)
                     /locus_tag="IXT53_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ4904680.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(40959..43052)
                     /locus_tag="IXT53_000833"
     CDS             complement(40959..43052)
                     /locus_tag="IXT53_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ4904681.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
BASE COUNT        12612 a         9151 c         7717 g        14199 t
ORIGIN      
        1 tattcaaaaa aaatggactg cgacacgact tttgtcacag tcccttcgtg tgtttttctg
       61 ccttctcaaa aggatgacag tttctcactc ttttcctatt tcttctattc aatcaaataa
      121 ctcattttat atacatctaa aaattcacct agatctgttc ttgcccctct aggcatgacc
      181 gcttcgattt ggaagcccat ttttcggtaa aggtgtacgg ctctttcatt acgaacttga
      241 acgtcaagct ccaatcgaaa aagtacaccc atttccttcg cccaactgat gatttcttct
      301 agcataagag tgcctagtcc cattccccaa tattcctgca gaatactgat accgacttct
      361 cccacgtgag acaaacgaaa ttgactgtct gccttgacag atgctgttcc aataatcgtc
      421 ccttcataga ttgcaagcag caataaatta ttattgcttt cgtatatata atccaactct
      481 tcttctaagg tttctggtgg taacaggagt tctgcttcat ccaaaactaa aaattctgtc
      541 tccttattaa cttgtcccat catcaaaaga atgtcttttg catctcctgg cactgcttcc
      601 cgcaagatga tcccaacttc ttcactcatg attttcagcc catttttcta gaattcgctg
      661 tttgaaggtt tcactttgct ccccgtatga gattagtttc acataccgct tttctaaatc
      721 tgtatcactt ttttctaaaa tcagttccaa ataatcctcc ggcagtgctt cttctaatag
      781 atttccccat tcaacaacgg aaagcccatc accttcaaaa tattcatcta accctaaatc
      841 tgctccgcta gctgctaccc gataaatatc catatgatac aacggaatcc gaccttgact
      901 atattctcta ataatcgtgt atgtcggact tttgatcatt tgcgttatcc ctaatccttg
      961 ggcaattcct ttcgtcaacg ttgttttacc cgcacctaga tcaccagtca atatcagatt
     1021 gtcacctggc attgcggaat gaccaattgc ctctgcgaat tgttctgtca ttgttaatcc
     1081 tgatagttcg atcatttttc atcttctccc gctcttcttt ctttttgcca gtatatcgcg
     1141 taatgaagtt tttttaaaga aaaaatgaac tagtcaagaa cagcagctgc tgtttttggc
     1201 tagttcgcgt gtatttgtta atcaatcaat gtttgtgctg ctgtgataat cgataattta
     1261 tatacatctt cttcatttgc tccacgagac aaatcagaaa ccggtttgtt caagccttgt
     1321 agaattggtc cgattgcttc aaagttaccg aaacgctgag caattttata gccgatattt
     1381 cctgactgca attctggaaa tacaaagact gttgcttgac cagctacttc tgaatctggc
     1441 gctttcagtt gtgcaactga aggaacataa gaagcatcaa attgcaattc gccgtcgatc
     1501 atgtattgtg gcgctagttt tttagcgatt ttagttgctt caactacttt gtctacttct
     1561 ggcgatttcg ctgagccttt tgtcgagaag ctcatcattg ctacttttgg ttcgatatcg
     1621 aacaattctg ctgtttttgc agaatctacg gcgatttcag ccaattcttg cgcagttgga
     1681 ttcacgttga tcgcacaatc cgaaaagaca tatttttctt gatctcttcc acgtaccatg
     1741 atcatcgctc cgcttgtccg actgatgcca ggctttgttt tgatgatctg aagtgctggg
     1801 cgaacagtgt cgccagtcga atggatcgct ccagagacca ttccgtctgc aatccccata
     1861 tacgtaagca tcgtaccaaa gtaattcaca tcttttaaaa tcttttctgc ttgttctttg
     1921 gatgcctttc catttctacg ttcaacaaaa gtatccacca tttcatccca cttatcgtaa
     1981 ttatcagggc tgatgatcgt aaagctagat gttttgattc ctctggcatg cgctgcattt
     2041 cttatttctt cttcgttccc tactaaaatg ggttccacaa gttcttctgc tttcaaccgg
     2101 gcagctgcgc ctaaaatacg agcatcagta gcttccggga aaacgatttt gatcccacgg
     2161 cgaacaattt taaatcttaa actatcaaac aattccacaa gatgacctcc agacgttttt
     2221 atatgtatta tcatacacta ttccagaaaa aaagaacagt ttattttatc tgtatcagtc
     2281 aaaaatgctg gaaaaattaa gtttttcaca tacaaagcga actgcactat aacaggaaag
     2341 cgctgtttta ttacactgtt tcaggcagtt gccaatcaat cggttcttct cctaacgcaa
     2401 ggagcgcatc attcgttttc gagaagggtt tggaaccaaa aaagccacga cttgcagata
     2461 acggactggg atgtggagat gttatgatga tatggcgact cttatcgatc attttgattt
     2521 tttcttgtgc cggttttccc cacaagataa acacgattgg tttttcccgt tcatttaatt
     2581 tttcaatgat cgtgtctgtt aatctttccc aacctttccc gcgatgagaa tacgcttgcc
     2641 cttcccgaac ggtaagaacg gtgttgagta aaaggactcc ctgtttagcc catgacacta
     2701 gatttccatg ttgaacaggt gcgattccaa gatcatcata aagctcttta tagatatttc
     2761 ttaacgatgg cgggatcttt acgccaggtt gcacagaaaa agataagcca tgagcttgat
     2821 tttctccatg ataaggatct tgtcctaaaa tcaccacttt aacttcttca taaggagtca
     2881 attccaatgc ttcatagata tgatacatat ctggatggat cttttgggac gcatattctt
     2941 tttttaaaaa ttctcttaga tgcagataat aatcttttga aaattctgag gacaaaactt
     3001 cttgccaact gttatgaatg atttctttca aaaactccac ctcctaataa aaagaataac
     3061 tcacattttc ttcaatcaca agggacaatc ttcaccctca gcacttgatt aatatggtaa
     3121 actaggaaca agaagcagaa aggaacgata atcattatga tcaaattaat tgcatcagat
     3181 atggacggca cattattaga tgcccatatg agtgtttctc ctgaaaatgc cgaggcgata
     3241 cgttttgcaa atgatgccgg tgttgagttc atggtcgcta ctggtcgaaa ctatcaagaa
     3301 gctcgcgcag ctttagatga ggtaggaatc gattgtgcga tgatcacttt gaacggggca
     3361 caggtatttg ataaagaagg aaattcattg tttaccgtgt cgattccaaa taatcaagca
     3421 atctctgttt tagatattct agatgctaac ggtatttact atgaagtagc aaccaacgat
     3481 ggtctttatt ctgaaaatca agcgaaacga attgaaagtt ttgcttccat ggtggctacc
     3541 catttacctc accttacgta taaaatggcg attgccatgg cctcagctaa gctggagtta
     3601 ttgcacatca catacgtgga cagtattcgt gctattttgg aagaaaagaa tctggaagtt
     3661 ttaaaaatta tttgttttca tacagaaggg ccagctgtat taggtccagt tggaaagcag
     3721 atttctcttt taggcgatct agccgttact tcctctggtc aaaacaatat cgaagtgaac
     3781 cataaaaacg ctcaaaaagg aattgctgtt gcccatgtcg ctcacgagcg tgggatttct
     3841 ttagaagaag tcatgacgat tggcgataac ttcaatgatg tcagcatgct gcaagtagca
     3901 ggagtcagtt tcgcaatggg aaatgcagaa atcgaagtaa aagattatgc caaatataca
     3961 acggacacaa accttgaatc tggcgtagga aaagccatat tgcgcgcgat tgcagaagac
     4021 ttgtagtttt atctagaaaa gagggatata gatgtcagaa tttttcattc aggaaaagca
     4081 gcttggacgt gcaactcgga caattgtcaa aaacgaaaaa ggacattccc tgtttttgat
     4141 ggttggtcgc tggggaacaa agggcgatgc attgtctctt tatgccatga atggcgatct
     4201 cgttgctcac atcaagcaga taagcctgac attcggtacg cgttttgagc tgtataaaga
     4261 gttcgaaaag gtgggaacca tgcagaaaat attcaattgg cccggtgact tttattatat
     4321 tcgtagactt cactggacag tccaaggtga tatctataat catcaatatc aaatccatca
     4381 tttcaatcag caaatcatga aaatggataa agcaacactt ttttcaggag attactatgt
     4441 attggatgtc tcaaatgatg aagatgctcc agtttgtatt tgtattgctg cggttttaga
     4501 ttactggctc taccgtaaag acaaaagtcg cgataagcct gtttcttacc gacttaactt
     4561 aagttaaaaa gatgtacctg aaatgcggaa atacgcattt caggtacatc tttttagata
     4621 ttcttttgca tatgatcata aagatgacca ctgatcatcc gctgaccgac aactttgaag
     4681 tcatggcgtt cgtataattt tttagccttt ggattttttt catccacact tagaccgatg
     4741 acggagcgat tagctttctt cgccaattta ggtaatgctt ctaataatcg cgaaccgatg
     4801 ccttgtcctc taaaatcttc tgatacactg atagagtcca aataccattc gttgggaaac
     4861 gtttctttat caatgaacaa acgtgtagac gcatctagct gatatttctc taagataggt
     4921 accagtggct gatcgatgat tgattcttct tcatctgtat aaccgtaagc aactcctgca
     4981 actttaccgt ctatttcttc tacgatacct cgtgaataac tgtaacgata atttggatca
     5041 gtgattgctt cttctaaaat ctctaaagtc ttttgttccc ctgtctgctt caaaaatggc
     5101 agttccatat cttttagaat cactaagatc aatggggcga tttctggagc atcttctttt
     5161 ctcgcaaaac gaatcatagt ttcctctcct tttctccatt ctaacatgca gaaaaagaaa
     5221 tcgcaaaatc gttattttaa aaaatcttca atcttttcag taatgaaata accatgagta
     5281 acgattcttg ccgcttcagt tcggcaataa gggcagacga ctggcatttc tttatcatta
     5341 tgttcgacta cattattgac ttcttcgtat gcaaattttt ttccgcaatt agggcatttc
     5401 attttttcca tgttatctca acttcctttc atctttactc tcttagctta ctcttatatt
     5461 gtaaaaaaga gaagcaatat ccttcttcta tgcagatatc catacctgac ggtcctgttc
     5521 tccatctatt aatcggactt tataataagg acgttcatct ttgactagaa gtgtccactc
     5581 tttcactttt gcttttttcc cgtagtagct gactgcttcc ttcatcgctg cttgcggttg
     5641 tttgaactct tccgtttttt ctatatcaaa aagatgttct gcggcctttt ccgtgtcatt
     5701 tcgtacggtt gttttcccag tggatggatt gactgctatg acttgtcctt cacaaaaaaa
     5761 ctggtattca tatgttgcat tcgtaggatt ctccaaatct ttttcaactg caaaatgaat
     5821 agcagatatg ttatgttctt ttgtttcgtt ttttacgatt gctgctgctt ttttcaatga
     5881 gatccgatat tcctggtcac taatatcatc catgtgaaaa gcggcattga gcagtcccgt
     5941 cataccggta atccctatta cgatgagggc agcaacacca aacatcattt ttttcattct
     6001 tcattctcct gtttcttttt tttaaagaat atcaagaaat gaacgaatga aaaatcgaat
     6061 gaaacgaaaa aaaagtgaca attttgttat ctttattcgt gcagttctaa tggcaatccg
     6121 tctggatcaa agaaaaacgt catttttttt cctgtaaagg tatcttctct aatcggttct
     6181 gattcgatcc ctaaggactg caattctgca attgtttctt caatatcgtc tactttgaaa
     6241 gcaagatgac gcaagccaca agcttctgga tagtttggtc gttgtggcgc attagcaatt
     6301 ccaaaaatct ccaattcagc acttcctagt ttcagatcta atttatagtc atctcgttct
     6361 ttgcggtaat tttctctgat tacttcaaat cctaatttat taacataaaa atcacgtgat
     6421 ttctgataat ctgaaacaat gatggctata tgatggatcg ttgtaaggtt catatgagca
     6481 ctcctattct tcgtttaagc tcggatctat attaatctcc agttccattg taaatgtgtc
     6541 agattgaaaa aaatagctgt ttttcctttt tataagaaaa aaggcaaaaa aaacagaaag
     6601 taggtcctcc aagacactct tcctgttccc aatataataa atgaaagtaa gaaattatat
     6661 tttcaagaaa acaaacagaa aattatcata acacaaatta tttataattg aaagactagt
     6721 tttttttcaa gaaaagcata caatagattg tgttagtaaa tacagaacac actacattta
     6781 gtgtcttaag gagtcctcat acatgaagtc atattttcag tttttagctc atcagctaaa
     6841 aggctggccg caacaaaatt attatctttt ctttttcagt cttggctgcc aggtcatgac
     6901 tcttgtcaat agtcccatca cttggctgtc cgttattaca tttatcggta ccacgcttgg
     6961 tgtattgtgt attttatcca tcaatgcggc aaaatcagtc aacggtattt taggtatttt
     7021 gtctgcgatt tgttttatta tcgttgggtt ttcagcaaag aactatttaa gtatcggtga
     7081 acagattgct tatatgatca cattggatat ccccgtattg ttaagtaaag aatggaatca
     7141 taatatggct tcaaaaatcc gaaaattcac tggaaaaagc tggtttatcg caatcatcag
     7201 tactttcctt gtctatcttg tttcaggatt cctgattggt cgttatacag atgatcctcg
     7261 gccgtgggta gatgccattg ctttttctat tagcttgact gctggtatca tcagtttctt
     7321 gcgttacaac aaccaatatt actggtggtt agcttcaggg cttgctcaaa tggtcctttg
     7381 gtatatttca tttacgcatg gatctgcaag tttggcgatg ttcgtgaaca gctctgttta
     7441 cttgctgaat gatgtccttg catttaccgt ttcaccttgg tataaccaaa aagaacgtga
     7501 acgtatgcaa aaaatggaag aagaatacta caacgcacaa caacacgcat aaaaaaactg
     7561 agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg
     7621 aatcagaagc aactccttcg gaaataagct gaaattcaca aaaatttgaa aagcaatttt
     7681 cgtgaatttc ttcttatttc ttggagttaa acgcttctgt cccagcctct attttttata
     7741 gtaattcctt cagatacttt cttgcctcat cattgaaacg cagtgattct tctaacttct
     7801 gataatattc caacgcttgt ttctgagctt cagtaaaacg ataatgtttt cctttccatt
     7861 tgtcgattgt ctcttgcaca tttccagatt ggatatcttt cactactagt tgccattttt
     7921 cttttgagac tgtacggtct ttcttatcag cagattgtat agcttctcgt tctttacgca
     7981 atacttttaa aaaaccattt ctgactatcc gaggaatgct gtcgaagaac ccttctgtaa
     8041 aaccatagcg acaatattgc ttccaaataa atagatagat ttttggttgg atgaatcgtt
     8101 ggtttttcgc aaaatattcc atctgagcag tgacttctgg tgttcgttga cgttttgtct
     8161 ttctcaacac ggaaataaat gtttcaaaat aagcattagg atgtactggc acttcttcat
     8221 atacgatgga ttgccaatct ttacgtaaaa atgcgcgata gttgatcaag aattcttcta
     8281 catcctttac taattcttct gcaaagtcgt caaatagttc tttttttatc tgtcgctttt
     8341 ttgactgccc attgatgtgt tcacttattt tcttgatttc attttccaat gcttgataat
     8401 ccgctatgta aatcggcatt tctacttggc gaaattctgc gagccgctga gctaatttta
     8461 tatccggtaa atatggctgt ttcatccgac gattttggta caagacaaaa cagtattttt
     8521 gccatagtcc ttcaggaaaa tcgatattct tctgcttaag ccataataat tcgtctaagg
     8581 ccagatcaac ttttgtcatt ccctgatgga tcgaagctgc cactgtcgga gagacatgca
     8641 ggtgatcggc aaagtggttg atgcctagtt ttaggatctt tccggttttt ggcgatttaa
     8701 cgatgtattg cacttttaat tctctaccac actcacaata caatttttgc tggcttcgtt
     8761 ttgttcgaaa aaacggattg atcttgaagt cagaaaacac ccaatctgtt gcagctaaat
     8821 aatgttcttc cataaaacga gagctgatta gatatcggcg gtgcttatcg actagttccc
     8881 gctgtttttt cgttaatttt gcatagatct cccaacgttg actgcttgtg tattgtttag
     8941 aaattttgat gggatagctt ttgctgattt cttttggaaa ccattcttca aagacggatt
     9001 gccattcttc cacggctgtt tctttccttt ctaattacaa aaagctggct actaacataa
     9061 cgttagcaac cagccagtgt ttcttgctaa attagtgagt tgttccggca tttccggaaa
     9121 tatcttcttt attttctact gctgcttcaa ctgcataagt caaagaagaa gcaatgtctt
     9181 ctaaagacat agaaggcgta cccactggac gatcgatcac ttgatcgggt gagaatggca
     9241 cgtggatgaa cccacctcga attgccggga attgtgtttc gatcaaatga agcaaacgat
     9301 acatgatatc attgcagaca aatgttcctg ctgtataaga gatgtacgct ggtagaccat
     9361 gatcttgtac attttttgtc attgctttga ttggcaatga ggtaaagtaa gcggctggac
     9421 cgttttcttc taaagccgta tcgaatggct gatttccttc attatcaggg attcttgctt
     9481 ctgctagatt gattgcgaca cgttcgaacg agactgctga ccgcccacct gcttgtccga
     9541 cacagatcac gacatctggt tggtgctgtt ggatggcttc tttgactact tgactgcttt
     9601 tttcgaatac ggtcggtact tccactttga tgatttcagc accgctgatt tcatctggca
     9661 ttttttttac tgcttcatag gctggattca ctttgtcccc accaaatgga tcaaaacctg
     9721 ttactagtac tttcatctgt tgttcctccg tccgattgat ttaacatatg gttatctttt
     9781 atcataccat tttccttgaa cagcgtaaat aaaggaaaca atagtttaaa taacataaca
     9841 gatcaaactt aatatattct tataaaaaaa tagtgttcca ctacaatgtt agaagaggtt
     9901 gcgacaatta ttctgtcaca acccctctct tctattattt aaatagaatc atataagcaa
     9961 tttggaaaac tagcataaat aacgcaataa acaactggtt tttgatcact ccgtatttgt
    10021 ctttcatctc cagcatcgct acaggcacga tattgaagtt agcagccatc ggtgtcagca
    10081 gggtcccaca aaaaccgcaa gtcaatgcaa ccattcctat taacgctgga ttcgctccat
    10141 gactaaatac gaatggtgca ccgatcccaa ccgtcatcac ggtgatggcc gcaaatgcat
    10201 tccccatgat taccgtaaat atcaccatgc caagagcgta aatgatgatg ccgatgttca
    10261 catttccttc tggaacgatc gtacctactg cattggaaat cactgtccca actccagcgc
    10321 ttgtaaatac cgcacctaga gaagctaaaa gcattggcaa catgcttaac ggccctactg
    10381 tgcccagcat atctgctgca tcatctaaaa atgtacttgg tcgattatct cgagaaaaga
    10441 acatcaagat tagcatggct gcaaaaacac cgacgcccac gccgactaaa gcacctaaat
    10501 tggtaaatag agcaaacaag atcgcaaaga caccgatact caaagctgga ataaaaattt
    10561 tcattcctag tttgtctgac attttttcca tatattcttt tgatggaagc cggctttctc
    10621 ctttgctgac tttcttagca attgctggta tggtcatcgc aaagatcaag ccaccgttca
    10681 cgattgccgg aagccatctt cctcctgcaa taactacccc taagaccgtc cagaaaaagg
    10741 ctgacccata acgatgagga ttggttttat ctgttaaatt tcttactcca gtgtaaatca
    10801 atacgagtcc catcacgata taaaggatct ccagtaattt atcgcctaat aaaatatcag
    10861 gatttgtaaa aaagtccatc ttattgcccc tttcctggat aatatttctt cgctaatttc
    10921 ttttctttaa gatagaagta gacacttgcc acaactaaag ccgtgatagc cactggaact
    10981 tccactaaag caagctgtcc caatgttacg tcatatccta gatctttcag tgttgattgg
    11041 actagcaacg cccctgctcc tccgatgaat agtacttggc caaagaacca gcagacattt
    11101 tccatagctg aagccatacc ttttagttct tcttcatgct taggatggac ttctaaccct
    11161 tttgattcga tggtccccaa tgccatcggt aaaatgattg gacgaacaaa tccagcaact
    11221 ccaccaaaac tcacgttaaa agccgcaaag atcattcgaa agatcccata aatatctaca
    11281 atcataccag cagatacttt cttgaatcgc ttgatcaagg aagccgctga ttctttcaga
    11341 ccatttcttt caagcgttcc tgttgccaac ataatgatga taaaaatcgc catcccgcga
    11401 ttgtttacaa aacttgtacc taaaagttct aacattcctt gaatgccaag accgccagtc
    11461 aaagctgtaa caactaaagc aaccattacg atcaatatcg agtctaattt aaatgcaaac
    11521 ccgagaataa caatcaagac gcctaaaagg ctaagataat tttccatata ttttcctcca
    11581 taaaatgaca atattttttt aattataaag caaaacacat aaaaaatata gttgttttct
    11641 cacgaaatag acactatttt taaatatttt tcgttatttt tttatcacaa aaattatctt
    11701 acttattgta aggtaaagct ttttttaatt tattgcccga taatatatag aaataaggtc
    11761 tagttactgt tttttttaaa tgattaataa ataacaactt ctttctatga aattgcaaag
    11821 cattcatctt cttttccgct aacgatatat ccgtcagtgc ttggtacact ttactaaaaa
    11881 gtatgagatt gccgatatct ggataggtgt gctatactac agaaaaagat atacaatctg
    11941 ccaccgggta ggataaagag gatgatcatt ccattaggtc tttgaaggga tgattttcct
    12001 ctttttgtgt atttttttat agttaaggag gaatgtaagt atggcatgga tggaattaat
    12061 catagcagga attttggaag tattctggtc aacgatgatg aaatggagtg atggtttttc
    12121 taaaatcaat tattcttttt acacagtgat tggcatgatt gctagttttt actttttgtc
    12181 aaaggcaatc aaaagtttgc cgatgagtct ctcttatcct atctggacag ggattggcgc
    12241 agttggttcg gtcataattg gtgtggtctt gtttcatgat aagctgaatc tttccacttg
    12301 gttctttgtg gggatgctac tcatcagtat cattggcatc aagattacta gcggccacta
    12361 gacacttgga ataagtactg atcagaaaaa ataagccttt ttatttaata ttcaattttt
    12421 ttatgaaaaa ataagaaata agtccaatga cctagcgact tctccactca tcaggtgtat
    12481 gcttgtacta tctatagaaa aggagtgaat atttttgaaa agtctttcgg tccaagaatt
    12541 atccaagaac tttggtcaca aacaagccct cagcgatgtc agttttagct tatcccctgg
    12601 caaaatcgtt ggattagtag gtcctaatgg cgctggaaaa acaacgatca tgaaagctat
    12661 tttaggactg atccattact cagatggaac aatctgtatt ggtgaaaatg agatttctcc
    12721 ctctagtcat aaagggttgg aacaagtagg agcattgatc gaatacccgg gtatctatcc
    12781 attcttaaca ggctatgatc atctgaagtt attctctgaa accaacgatg tatctgctat
    12841 cgacactatc gtgaatcagc tcaagatgga aaagtacatt cacaaaaaag cgaaatccta
    12901 ctcgttggga atgaaacaaa aattgggaat tgctcttgcc ttactgaacc atccgtctat
    12961 cgttatttta gatgagccga tgaacggttt ggatccacaa gcaacaagag atgtgcgaaa
    13021 aatcattacc gacttggcta ctcagggcac ctctttcttg atctccagtc acttactcag
    13081 cgagcttgaa aaaatggtcg atgagctgat cctgatcaat caaggaaaga tcatcaaaca
    13141 atgtacaatg gctgaattga accaaacagc gcaagatttc atcgttatct ctacaacaaa
    13201 tgatctagct gctcgaacga tcttgacaga agctggatat actttggaag atcctgataa
    13261 aatcaagctt ttaaaagaaa acgaagaatt attaggaacc gtgatccagc tattgtctgc
    13321 acaaaatatc catgtgacag acgtccagca tctgacgaat gatttggaat cttctctatt
    13381 ggaaattctg gattcagtaa agggggaaga agaaaaatga cattgcttgt aaaacaagaa
    13441 ttatttaaat tgatcaagaa aaagtcaact gctgttttat ctgttctatt agttgttttg
    13501 ctcataggaa cagcacttct agccaaaaaa tacacgacga tcattgatcc agttgaaatg
    13561 actgctcagc tttttagtgc cacatcttgg attgtcttta tcatgattgc agctgcaagc
    13621 acgatcattt cgatggaagc acaatatggt acattgaaaa atctccttta tcgaaagtat
    13681 tctcgaggag aaatattagt aagcaaatgg atcacattag ttatttattc tgtttattta
    13741 taccttttag ctattattgt cactgtgttg atgaaactga ttcttttccc ttcgatttcc
    13801 ttcacggaga aggtttctac tggtcaaaca ctgatccaaa gcttgttcac ttatactttg
    13861 ggtagttata tcggtttatg gctgatttta agtttagtgt tgatgattgc ttgctttatc
    13921 aatagttccg gcgcatccat ctcagcagga atcgtctttt attttgcttc gtcaatcata
    13981 tctggtatcc ttattgcact catccaaaaa tgggagtgga tcaaatggaa tccgatcagt
    14041 atgttgaatc tgcaaaacca aattggaaac gaagagatca tgaagcaatt gactcatctt
    14101 tctactaatc aaatgctatt cggtaattta gcttacatcg ttttgttctt agcattagga
    14161 tatctcgtat ttaagcgaaa aaatgtatga tgactcatca aaaaagaggc tgtgaaagag
    14221 acgttcagcc ttattgccga ttggctgtct cttaaacacc gtttattctg aaaaaggagc
    14281 tgtgacaaca tttgtgtcac agctcctttc ctagtatgtt tttgaataaa cgacaggctc
    14341 acaacctctc tttatacttc ttttatactt ctatacgtga agcatcccag tggcaaataa
    14401 aaccattgca atcactccga ccatcgtcac acctataagt aaaatcttat ctaagtggat
    14461 atgttgtaca ggctcgtttt gctccttacg tgctttgagg taaaaataaa tgcctggcaa
    14521 gaaaagaaca agaacaagca ttacttcatt aaaggctacg actagcatag cccatacttg
    14581 aaaggcactt gctaaaagcc ctactaaaaa ttggacccat tctttccgct gataagaaag
    14641 tttcatttgg tacaatccta agaaaagcca cgtgaagaaa atagctgctg taccaagtgt
    14701 gtaagcaaag ttataagcac tgtcagtgaa taataatgaa agtaaaaaga cattcgtaca
    14761 taacgtcgtc aatacgatag cataagttgg tgccccattt ttgttgagtt ttccccatgc
    14821 tttaggcaaa agtccgtctt cagccatgtt tcccattgtt tcaatcggta agatcgtcca
    14881 tgagagccaa acaccaacta aagaaatgat caaaccgccg ttgatcacga ctgctcccca
    14941 tttaccaacc atcatttcaa acacatagcc catagcaggc tgcgccttga tcgtttgcag
    15001 ctgattcatc gtcattgtcc catatggaag catggaaacc aagacgtaaa taataatcaa
    15061 gccgaatatc cctagaatcg ttgctttccc aacatctgat tgtttttttg ctcgtgaacc
    15121 tacgacagag gctccttcaa taccaacaaa tacccaaagc atcaccatga tgcaactcaa
    15181 tacttgactg gaaaacggga tcggctgtcc attcgcattc aatataatgt cgctagaaaa
    15241 gttatcaatg aattgttgga tcgaaaacgt gctgatagct actacaataa ataaaaataa
    15301 tggcactaat ttacagaatg tgacgatagc attgacgaaa ctagcatttt cgactccccg
    15361 attcactagc catgcatatc cccataaaaa gactgtactc atcacgatcg atgccagatt
    15421 ttgcccacct tcaaatactg ggaagaaata tcctaatgcg ctcatcaaca atgttgcaaa
    15481 agctacgttg cctaaccagc atgtgagcca gtaaccccaa ccgctgataa aaccagcgaa
    15541 acggccaaaa ccttcttctg cgtaactaaa gatcccgcct tttaaatctg gacgtttttt
    15601 attgatatta ttgatcgaca aggagagcat caacattgct gttcctacga gtacccaact
    15661 gaaaatagct gggccgccag ccgatccgga gatgtccgtc ataataccaa atacgccacc
    15721 tccaatcgct gaaccaatta ctaaggcagc aagtggaatt aaaccaattc cttttttctg
    15781 ttgctctgta tccatgtaag catcctccaa aataaaaccg cttttatcaa aaagtataac
    15841 aaaccttttt tatttttact taattaataa aaactttaat agaaatataa ttctaccggc
    15901 ttttttctca ttatacaagc aaagcgccta gtccctttcc aaattacacc accaaaaaac
    15961 ttaaaaaaga tgctatacta aaaggaaaga attggaggat gatccatgaa cattttatat
    16021 atatcgattt caggaaatac gcgtgccttt gccaaacatc tagcagaata tgctgaaaaa
    16081 atgcatgcag aagatcctgt caatcctgaa gttactttaa aagaaatcca tgaaaatagt
    16141 gattttacga aagaaacaga accatttttt actttcgttc ctacttattt agacggcgga
    16201 aatggtttag ataacggtga tacagaaatc ttgaccgaaa cgatgcgtga atatctggaa
    16261 catgaagaca atcataaact ttgtcttggt gtagtcggta gcggaaataa aaacttcaat
    16321 aatcagtatt gtctaacagc caaacaatat gcacaaacat ttggctttcc tttcttggca
    16381 gattatgaat tacggggaac accatctgat gtgacacgaa tttatcagat tttagcagaa
    16441 aacaataaat aataaaaaag aagaagctga aaaatagatt tcagtttctt ctttcgtttc
    16501 tattgtttct atcatattaa gctttgacat ccatcacagg tgcttttgct tttgctttac
    16561 ctgttttttc caagacaaag ttttttacac gatccatatt ggttaccaag acgatcatgg
    16621 atgtttcttt gcctgctgct ttgatcgctt ctaagtcagc ttctgctaca agcgtatcag
    16681 gtgtcacttg ctgcccttct gttactttca catcaaatgg cgtaccgcct aagtcaactg
    16741 tgttgattcc catatgaagc aagatttcta aaccatttgg cgttgtgatc ccaatagcgt
    16801 gtttcgttgg gaagacagag ctgactaccc cagtaactgg agaatagata tgtccgtttt
    16861 ctggttctac aaagtagcct tcacccatca tcttttgtgc aaaaactgga tcacttgctt
    16921 tatctaaaga aacaactgtt ccgtcagcaa ctgatgagag gatagtcaca ctttcagctg
    16981 aagtttgagg tactactttt tcaggcactt catttactgt ggctgtttca gtcgctaatt
    17041 cttctttttc taaacggtct ttaggaatac cgaagaagaa tgtagcaacg aagccaccag
    17101 cataagctgc caataatcca aggatgtagc ctaaccattt accgtcagag atcaatggga
    17161 tcaaagcggc tcctgaaggt ccaatagcga tagccccgac atttccgatc attccgatca
    17221 ctgccccgcc gatacctcca ccgatacaag cagtaataaa tgggcgtcct agaggtaatg
    17281 ttactccgta aatcaatggt tcgccgattc ctaggatccc gactggtaaa gctcctttga
    17341 tcatttctgt taattcttta tcttttttgc agcgaatcca taatgccaat gctgcaccca
    17401 cttgtcctgc accagccatc gcaagaattg gaagcaatag cgtacgacca gtttcatcaa
    17461 tcatttgcac atggattgga gtcaaaattt gatgcagtcc gaacataacc atcggcaaga
    17521 ataatgtgcc tagaacgaag ccggcaaatg ctccaccaac gccaagtacc cagttgatcc
    17581 caccgaccat gccatcagaa atgaaacctg ctaaaggcat gatgaagaaa atttcaatca
    17641 gaccgatggc aatcaatgaa agagttggtg taacgatgat atctaccgca tcaggtacta
    17701 ctttatgcag ttttttctca acgattgaca atagccaaac agcgaagatg acacctagga
    17761 tcccaccttg tccagctgca agagcttcac cagtaaataa gtttttcagt ggtgcttccg
    17821 gattcatccc tgtcagcatc acaacggccc cgataactcc accaagagtt ggtgttgcac
    17881 caaaaacttg tgcagcattg atacctgtat agataactag gtagctaaac atcccgtttt
    17941 tcaagatgtt catcacatcg atatattgtt gccatgttgc tgcatcaagt gttccggctg
    18001 tgactaagtt tgctaaaact gcggcaatcc ctgccacgat cccagtaccg acaaatgcag
    18061 gaatcattgg cacaaagata ttagaaatgt cttttaatac cgatttgatt ttagatggtt
    18121 tttgtttcga tttttgtgcc gctttcattt cttgtgcccg ttcgtttaca cggtcttttc
    18181 cagatgctga tgaatttgct gtagctgtac ttacagaaga aagtttttct cctaatttca
    18241 cgcctgccaa atcaaccatt tgctgtgcta ctttgttcac tttcccaggt ccgatgatga
    18301 cttgaagctg ttcatcttct actacaccaa gcactcctgg aatcgctttt aattcagaga
    18361 catttacaag atcatcattg atgatcttca tacgcacacg agtcatgcag tggacaatgc
    18421 tttctacatt gcccatgccg cctgcccctt tataaatttg ttttgccagt tcttccaaag
    18481 tcagttcgtt ttttgccatg gtcttttccc cctcataagg tcccttttac aaatccgttt
    18541 gcttcagcta attttgcttg agcttcctct ttatcactat ttgtcaaaat catgacgata
    18601 gccagcttaa cattttgatc tgcttcttca aagtactttt cagctgtttc gtaatcagct
    18661 tctgtcgctt gcatgatgat tcgttttgag cgttcgatta gtttttcatt tgttggtttg
    18721 acatcgacca tcaagttatt gaatacttta cctataccaa tcatactgat agtagaaatc
    18781 atattcaaga taagtttttg tgctgttcct gacttcaatc gagtagagcc tgtcaaaaat
    18841 tctggacctg catcgacttc gattgggaaa tccgcgtgtt tactgatttc ggcttgttta
    18901 ttgcaagaaa tcgttcctgt gacagcaccg atcgattttg cgtaatctaa tccaccgatc
    18961 acatatggtg tacgaccgct agctgcgata ccaataacta tatcattttt tgtcaaattc
    19021 agcttgatta gatcttcttg ccctagctca taagaatctt cggccccttc gacagctaca
    19081 gtcattgctt cttctccacc agcaattaga cctatgacca tttccggttc tacaccgaat
    19141 gtcggtacgc attcagcagc atctaacaca cctagtcttc cacttgttcc ggctcccata
    19201 taaatcagac gtccaccttt tttaaaggac tcaatcgtgt tagcaatcac tggttcgata
    19261 cttgtcagtt ctttttcaac cgcagcggcc actttctgat cttctttgtt cattagttga
    19321 acggcttgag aaatactcat ctcatctaat ccaaaagttt cttggtttct gcgttccgtt
    19381 gttaggttct ctaaattgat tttgctcatt tttatcttcc ttcttttctt ataaattgat
    19441 agttcatacc agctttgatt tggttgatca atggtagctc ttctttgata actttagcta
    19501 ctacattcac tttttcatcc gctggcaaat catatttggt taattggatc tcacccatat
    19561 agcgcaaata cttttcgtta tctaaagtaa tagacccttt catacgcgta gctgtatttc
    19621 ttgctgggat aggcggaatc tctcgaaaac gtgcatctac gcttcgtatg acatctcttg
    19681 catcgtcttg ccgattcgta tgcttcccta atatatattc gtaaaattct tcatctacag
    19741 gttccgtatg gagcaagact gtctgttctt tttggaaaga agcaaattgc tcctgaacgt
    19801 tttctgacag acctgcatct ccgatgtaaa caatatccgt gttacttgct aatagatcta
    19861 gagcgcctgc aagaggatgg atccctcgat gttcttccaa agttggcaat ccttgatata
    19921 gtgggcctct caactctgta tctcctggta caaaaccttg gacaaggaga ccttgaattt
    19981 tgagccattg attttttttc tgataccatt ctttgtccag ccctgtttcg ggacgtggat
    20041 aataattatg ccaagcttca atctgtgaga aatcagcttc tgcttctttc aattcatcga
    20101 tatctttagg cgtgatcgtg ctagcattca aactgatttt caattgatgt gaccagtcgg
    20161 caatctgctg attggaaata tgataatcca ttcttaaacc tgttacacca attgctttca
    20221 aaggcgttag atcttctaat gaaaaacccg ctcgttccaa tgcctctcct gaaatatcga
    20281 ccatcaattc tagtttattc tttttcgctt cgcttcctag ttcgatcaat cgcttttgat
    20341 atgccgaaac atcatcttct ggtatatgca tggaagtgaa aataccgaca aagccattgg
    20401 atgccatttg gcggatatac tgtttcttct cttctgacaa agaatcgttc ataaatattg
    20461 aaaagccaaa catattatcc ctctttttct gtatccgtct tcatttctat gactagagta
    20521 tatatctaaa gaaacaaaat ttcaatactt ttattaaaat aattaaattt catttctaaa
    20581 ggttgagttt atattatcaa aagaattgtc ataacctaaa aaaggcactg ctaagcaaaa
    20641 gcagtgcctt gaaatatttt atttaaataa cacaaaaaca taattactca taagaaacca
    20701 attctgtatt atctaccatg aatgtaaaga acccctcatt aggcatcgga tgaaaataag
    20761 cattgatcac tcttatcaag gcattatggc aaactaacag atagacattt tcttcgtctt
    20821 cgatgcattc tttcaataaa ggaacgatcc gtgcatacac atctaagact gactccccat
    20881 ttgggaatcg tacagcgaac gctaaacgtg ctttttgaaa gttctcatct ttgcttggca
    20941 gtccatcata atccccaaaa tccatttcta ccaatcgttc atcaacggtc agcggcaagc
    21001 tgagacgatc agcaactgct tgtgctgtat ctcttgctcg ttgaagaggg gaatggatga
    21061 ttttcgtgat tggaacaggc aaatctacta ccttttctgc tagtttctgt gcctgcatct
    21121 gtcctttttc agtcagcggc acatctgccc gtccgcaaac taatccttgt gcgttccaag
    21181 ttgtttctcc gtgtctcgtt acataaagca tgtgattctc tccttcgttg gcaaagttcc
    21241 ccttaaccat accttgttgt tcttgagatt acaagggtta gattctctca agagaaataa
    21301 tctcagtaga tactttatcc aggaaggttt gatcatgttc cactagtaac atggcaggtt
    21361 tcacgttcaa gatcagttgt tctaattgtt cttgattgaa gacatccaaa taattcaatg
    21421 gttcatccca tgtatatagt tcagctggct gtgataaaga tttagccaat tccacttttt
    21481 tccgttggcc catactcatc tgctcgatct tgttatgaaa aacatctctt tccatcccaa
    21541 gctttcggag gttgttcaaa aatgcttggt agtctacttg gtttttctct gcaaattccg
    21601 ccaacgttcc tcgattgtct tcataatttt ggcttgcata actaatgctc agatgttttg
    21661 gctggtattt ttctcctata accttgccgt tgaatgcccc cagaagataa tggataatgg
    21721 atgactttcc tgcaccgtta ggacctgaaa tcgccacccg ctgatgaggc tcgattgtaa
    21781 aatgaattgg ctcgaataac agattttcat accctaattg aagatcttct acgcttaaaa
    21841 gtcgcttatg gtgagacgct tggctattca tcgtcaaaga atcgatatat tcgatatctt
    21901 ttaatagttt ttctttctcg ctgatctggg tctccatccg attcacgatt gctttggatc
    21961 gtttcatcgt ccgtgcagca tcagcaccca ctgctccttt attcactcgt ctagattcag
    22021 tatcgatgaa tccgacttgt ttctttgttt tatctccttc tcgggaacga gaccattcgg
    22081 ctttttcagc tgctgttttc tttagcctac tgacttcttt tttcaatttt tcattttgag
    22141 ccatttcaaa ttcatcacga agttttttct gttcttcata gatagagaaa ttcccttgat
    22201 aaagttccag ttgacttttt tcgattgcta aaacatggtc cactacttca tcgataaatc
    22261 cccggtcatg gctgaccacg atgaagcctt gttttttctt tttcaaataa gccgctactt
    22321 gttttctacc agagatatcc aaatgattcg ttggttcatc gattaacggg aaatgagtgt
    22381 catccacaaa taaaagtgcc agtaggactt ttgtcttctc tccccccgat agtgtgctga
    22441 attctctcca taagatttca ggatctgttt gcatcaattg gagctctctt tcgatttccc
    22501 atatctcaaa atccgtaata tcatttaaca cgtaataggt taaacgttct ttatcttttg
    22561 tctgttgcgg gaaataggca aattcttgct gatggattac ttgcccttga taaggtagtt
    22621 tgttttgtag aatattcagt aaagttgtct ttcctcgacc gtttcgtccg ataagtccta
    22681 gtttccattg tgtgtcaaaa tttagatttg cttgttcaaa taataatgtg ccttggctgt
    22741 cgtagccgaa tgtcagattt tttatttcga ttttagacat tttccatcac ctcgtaaaaa
    22801 aagaccagtc ctattttcag aactggctaa agaggatgca agggttggat acacacaaaa
    22861 agacccatac atcgaaaaaa ccaagccaat cccgaaaatc tgcacactgc ttccttgtct
    22921 ccacaaggta cagtctgaat gcagctttca tcaatcgact taatttttca atgcacggat
    22981 ccctcacttt gtcctatatt tgaaattacg atagcataaa gaaaatgatt ttgtcaacta
    23041 cttcgggagg atggtgaaaa atctgctaag tgtctgtcct aagatttttg ttttttctaa
    23101 tgtttgcctg tgttccattg cttcttctaa acctattgga cccaaatgaa tacttaagac
    23161 aattgggaga aaatcagaaa gtccttctag ctcttggtct ctacttccgc aaatagcgat
    23221 tattggtttt cgatattttt ttgcaagcac tgctacaccg tatggaactt ttccgtggat
    23281 cgtttgctga tcgatccgcc cttctcctgt aatgatgaca tctgcttgtt cgatcttttt
    23341 ttccaatcca gtgatttggc tgactaaatc gaaaccagaa accatcttcg ctcccaacaa
    23401 aaaaagggct ccaccaatac cgcctgctgc tccacttcct ggaatatcat ctaaatccac
    23461 ctcccagaaa tcttttattt gctgagcaat ctttcctgct tgtttatcta agaaagctag
    23521 cgtcgtatct attcctcctt tttgtcttcc aaattgccaa gcagctcctt gttgacctgt
    23581 atatggattg gtaacatctg cagcgataat caattttact ttagaaacaa gaacgtttct
    23641 tcgatcaaaa tgaagttttt tagtagataa taaaggattt ccttctgtgt acccttctaa
    23701 agaagcaccc aaggattgga gtaatcctag accaccatcg ctacacccgc ttcctcctaa
    23761 agtgacaata atttgttcta cttcttgaag cagtgcatct ttaataagtt ctcctaaggc
    23821 ataactggaa gcaagtcgga tagtttgatc cgttggttta tcaagtagat ctaaccccaa
    23881 tgcttgtgca gattcgatga ctgcggtgtt ttttccttgc caagttgtca ataaatagct
    23941 gatttttcgc tgacggaata agagatccat cccatcaatg gtacgccatg ttcccctgag
    24001 cgcgtaatag attgcttctg cagtattttc tccgccatct gcaatcggca cattgatttt
    24061 tcttccttta cgatcttccc agccatttag aactgcctga tttagttcaa atgaagaagc
    24121 acttccttta aatgaatcaa ttgccgccaa aatatccatc tgtatccctc actttcttct
    24181 taagtaagac actttttttc atttttttca aacaaaacaa tgctattttc ttccattttt
    24241 ctatatacta aaagggaact cattgaataa aggagagaaa aaacatgaaa atcgcaatag
    24301 ctggggccgg agcaatgggc agccgaatcg gattgatgct acatcaaagc ggaaatgagg
    24361 tcctcttgat cgatcgttgg cctgctcaca ttgaagccat tcgtacaaat gggttgattg
    24421 ctgactttaa cggtaaagaa gtggtcgcaa aactgccaat ctattcacca gaagaaatca
    24481 tcgaaagcaa cgaacacgtg gacttgatcg tagcattaac aaaagccaat caattagatg
    24541 atatgttttg ttcgatccaa tccattatta ctgacaatac ttatgttttg tgtttactaa
    24601 atggattagg tcatgaagat gtactggaaa aatatgtgcc aaagaaaaat atcttatttg
    24661 ggatcacgat gtggacagct ggtctagctg gtcccggaaa agtcacattg ttaggtgatg
    24721 gagaaatcga actagagaac ttggaaccag aaggcgaagc atttacaaaa aaagttgtag
    24781 aagtctttca agaagcaaat ttaaatccga tctatagcca taacgttcgt tattccattt
    24841 ggcgaaaagc ttgtgtaaat ggaacattga atggtttatg cacgattttg gattgtaata
    24901 ttgctgaact aggcgcgcaa aaagcggcag agagcatggt tcgaacaatc gtatctgaat
    24961 ttgcttctat cgcagcaaaa gaaggaatca tactagatca agaagaagtg taccaacata
    25021 ttgcttctac ttatgatcct gacaacatcg ggttgcatta cccatctatg taccaagacc
    25081 tgatcaaaaa ccatcgtttg actgaaatcg actacatcaa tggtgccatt tggcgaaaag
    25141 gacaaaaata tgatattgct acaccatatt gtgccttttt gacccaatta gtccatgcaa
    25201 aagaaggaat ccttggcgca gaataaaagg ctctctgatg agaatcagct gaatcagcag
    25261 ctggttctct ttttttaagc ccaataaaag atcaaaaaga aaacacaact ctcatgatgc
    25321 cataagaatt gtgcttgttt gtttcagtac ataatctctt caaaatgtag gttttttatt
    25381 tgttttcaac agaggagagg tcatattcgt attgctcata aatagtggaa tcgttagtga
    25441 taaatcgttt tgcccaattt tctgcatcat aaaattccaa tagctctgat aggattggct
    25501 taaatggtgt atgcagaaat tccggtaatt catctaaatc acgttctcga agcaaaaaaa
    25561 tattttcagg acgataaaaa tgataatgag caatcgcttg attatccgtc actagtttct
    25621 tttgaaaata catggattca aatgtccgca aagtcactcc ttgctgaccc tcccgcaaca
    25681 gttccagaat cccgcgcgat tcccctgttt ttttcaggta ctcttcataa gtaagatatc
    25741 caggttgttc attccctcga tgtggaagaa tgaaatagtt ggtcgtaaca tctcttttta
    25801 taaattcctg cttatagctt tctgctcgtt cttttctccc attattcgtt gcaccaaaaa
    25861 atagatccgt ctttggttcg ctagaagaaa aattcatatg tttcgaataa aatgaactat
    25921 tatgtttcag cccaagttct tttgcttcca aaaaatcaaa atgataaatc tcatcgattg
    25981 ccggttcatc ttgtgcctgc ttcagcatcg cctcgtgttc tgctttgaag tgattccaaa
    26041 aaaataacac aatttttgca ttggtaaatg ggcgcacgcg tcgagccatc tccatatcaa
    26101 atccataatc taaaaaaacg atcagctgat aatttgccaa ctgttgtcta gctgatttaa
    26161 tgaattccga ctgtagtaaa tcttttccgg accaaacatc taaagagcgg atggaaggca
    26221 aaaaataaag tgatttattt tttgcaacta gcagtacgtc atttttgttc atcttttccg
    26281 cctcactttc tctgtcactt tttcctatga tagcctgttt aaatagtaaa aagccagtaa
    26341 aacgtttcct atatttgtat gaaaatttaa catttgtatg aaaatatgat gaaataaatc
    26401 attcttctat taaaattcgc ctaaaagttt tctgaatagt tgtaattgtc tgaaaatttg
    26461 ttatcattag tcattgttaa atattgctat ttattcttaa tataaatttt gattgtgaat
    26521 ttaaaagaaa gaggtcattt atggaagata aaaaattgaa aaaacaactt agctccagac
    26581 atatcaccat gttagcttta ggtggagcaa tcggtgcagg tctatttaaa ggaagtggcg
    26641 aagcgattgg gatcgccggc ccttctgtat tgattgcttt tttgattggt gggctgattt
    26701 tatatatcgt gatgaaaggt ctgggaaaaa tcgttttaag cggtggcgac acgcatcacg
    26761 gattatctgg tttgatacgc ccttatctag gtgctcattc tgctgatttt actgattggg
    26821 tctactggtc tatgtggatt atcaatatca tcgctgaagc tgttgcagct gcttcctttt
    26881 tgcagttgtg gttcccgcac attccagcct gggtatttgt ttttatgttg gctgttttaa
    26941 ctacgatcat caatctttat tcggtacggt tattcgcaga aacagaatat tggctggctt
    27001 ttgctaaaat cagtgtcatt atcttactga tcatttttgc tacttatctt gtcggtcagc
    27061 aaatgttggg cacaggcgtt ttccctaccc ttcagcaatt gactgaccat ggtgggttta
    27121 ctcctcatgg tatgaaggga atcatcagtt ctttgcttgt agttatctat tcttacggcg
    27181 gttctgaact gattgctatc acggtaagcg aagcagatga tcctaaacgt gcgattccta
    27241 aagcaatcaa aggtgtcatg ggaagaatca tttcctttta catcattcct ttgtttttgc
    27301 ttttgattct ttttccttgg aatactttag caggcacgaa tgtcagtcct tttgtgatgg
    27361 tatttgaaaa aatgaatatc ccatttgcag cggatatcgt caattttgtg attgtccttg
    27421 ctttgttctc atctatcaat tcgggtgtgt atgcatcttc aagaattttg ttcttccgtt
    27481 tgaaggatcg aaaaggttcc tctagaaaac tagctgtttt aaacaagcat caagtaccgc
    27541 aacgtgctgt tttattttgt accagtgttc tttatcttgg tgtagctctt tcttattttg
    27601 tcggtgacaa attatttggc tatctggcag gttccctttc gtatacagta ttattgatct
    27661 ggatcattat cagtgcggca tcctttgtac tggcactaag aaaaggaaca ttatgggata
    27721 aaggaatcag cttttttgca ttagctgtct taggattgat tttccttggt atcctgttca
    27781 cgaactccat tggggtaact gtattgactg gcctgcttta cctgttcatt tatttcagtt
    27841 atcaaaaaaa gaatgatgcg ttcgtattga cgaatgaatg aaatcgatag aaaattttat
    27901 tttatttatg tggaatagat ggagctattg aagaaaggag ttgtgacaaa agtcttgttg
    27961 caactccttt ttccgaataa acagtctcat gacctcttgg taaacggtgt tcaaaaaccc
    28021 tctgtgcaaa ataattgatt gttctccatg ctcatgacat gatagaagaa aggaatgaaa
    28081 aaaaggagtt tgttttaaat gaaaaagtat tggaagtttt tgttgatgat cggtgtttca
    28141 acggtagtta tgtttggtat gatgtacttg aatgtctatg cgctcgatca tatcttcttc
    28201 agtcgtacac gagtattcat ggcgttgatg atgggtgcag tcatggcagt catcatgctg
    28261 ttgttcatgt ggaaaatgta tgaaaacaaa aagataaatg ttatgatctt agccgctagt
    28321 cttcttgttt ttgccggttc cttgtttatg gtacgaagcc aaactactgt caacgacaca
    28381 gcttggatga aggccatgat tcctcatcat tctatcgcta tcctggcaag taaacgagct
    28441 gatttaaaag atcctgaagt aaaaaaatta gcggaagaaa tcattcaggc acaagaagaa
    28501 gagatcgaca aaatgaaaaa aatgatcaaa gaaatggaat gaccattatc cgtccaaata
    28561 aaaaaagagg atgttccaga agttatcagc ttcaagaaat aagaagaagc tacttctgga
    28621 acgccgtgaa taaggggttg tgaaaaagct gccctgcatc aagtaataag aacagccaaa
    28681 caaaaatagc ttctcctatt tttcgtttgg ttaggcttat taccgcagat gcaagctttt
    28741 gaacaccgtt tattaagagg ttgtaaaatc agcgttctga cctgagtatt agagagacaa
    28801 atggaaaaat agttcctcat attttgccat tttttgagct aataccgagg gtcagctgat
    28861 tgaacaccgt ttatcgagag gttgtgaaaa agtcgcttag tttcgcgtat taagaaaaat
    28921 tttgtgaaat ggctttccac atttttgtat aaatttggct taataccgag aaactggctt
    28981 ttgaataccg tttattcgta aaagggattg tgacaaaagt cttgtcacaa tcccttttta
    29041 tgtttttatt gttctaattg cgctgtcttg cttactgctt cttgcggttt cacaaatttt
    29101 tttgagattc cccaacctgt gaaggcactg accatcgaaa acacaggaga aagcaaactg
    29161 aaaaatacga atggcaagta agtgagtgta ggaataccaa atgtacttgc tacgaaactt
    29221 ccggcaattc cccaaggcac taagtagttg attactgttc caccatcttc tagcgttcta
    29281 cctaatacag ttggatcaaa tcctgctttt tcataagttt ctttaaatgc attaccaggc
    29341 agaataacag ataggaattg ttcaccaata aaaatattta ctccgatact tgtcatcaat
    29401 gttgtaaaaa tcaaatttgg tgtgttcgtc atctttcttg atactcgttc catcacggtt
    29461 gagatcaagc caaattcgat caacaatcca cctaatgaca aagttagaat aatcaatgac
    29521 acagtaggca tcatactttc gattcctccg cggctaagca gtgaatcgat ttgttggttt
    29581 ccagtatttg aaacaaaccc tgattcgatg accccagcca acttcgttaa ggaagtttgt
    29641 gggttttgaa taaaaatcat tcctactgct acagcgatat tcaacaaaat cgttaaaatt
    29701 gccggcacct ttttccatgc acacagtaac attaaaaata aaggaatcag tgaccagatt
    29761 gaaatagtaa agtttgcttc tagaacatca gtcacttcac gaatagatga aagacttgcg
    29821 gctttacttg tgtgtcctaa aatcatgaat aaaaagaaag aaacaacaaa tgccgggaca
    29881 gttgaccaca tcatatgctt gatgtgtttg aacaaatcgg catcaacaat agctgctgct
    29941 agatttgttg attcggataa tggagacatc ttatctccaa aaacggcacc tgaaacaata
    30001 gctccaacaa ccagcgctgg atttaagcct agtgttgtcc caataccgaa aaatgcaatc
    30061 ccaatcgttg acataacagt aaaggcactc cctacagcgc tacctacgat tgcacacaca
    30121 acaaatacag aagggacaaa ccactggaca ctgatcaatt tgaacccaat gaccattaga
    30181 gttggaatga tccctgcttg tatccataca gcaatcaatg aaccgactaa aataaaaata
    30241 aagattggga tgattcctgg tttgatgcca ttgatgattc cttcgtgcag ccaatctgtc
    30301 gggtatcttt ttatcactgc atacaacatg accattccga tcgctgctaa gatcgtgata
    30361 ttaggtgaga cctttaatcc gacaacgcca atcgcaatgc ctaaaacaat aaccattaat
    30421 acaagtgttg cttctaaaaa acttggtgtt cctttttttc ccatgataat ctctcctttt
    30481 ttagttgttc ttatgttcta aaaaagttaa gatatcggat tactccgccg caattcatcc
    30541 gcgattcctc ctttataaaa ataaaaagac ctgttttaaa ctaaggacga atgactccgc
    30601 ggtaccacct taattaaaaa cagatctgtt tttcactttc aaacttttat tcaaatgtat
    30661 ggactttcca tgtaattcgt caattaggct tgatcggttc acagcgacca ccgactttct
    30721 gaacaaaaca cctaaccgct actgcgtgaa aagttgactt gtacgaagtt taccttccat
    30781 aagatcattt gtcaatgaaa aagaaaatat tttttcattt ttctgaaaat tatttttata
    30841 actttcacac acaagtttat tttttactag aaagtagcgc cctcttgttt ttttcttgct
    30901 actatttctt gatgttcttg cttgattctt tctaatagct ttggctcttt caacagatca
    30961 agagctgtat tggcgagtag ttctgctgca atcgcaatag aagcaaggcc tttgtcactt
    31021 ttagcagcag ctttgaattc ttctgtgtgt ccggcaatat atgtctcaga gattgctacc
    31081 gtaggctgga ttgttggtat gacttggctg acatttccta catcagaaga accgagactt
    31141 gttctttgtt ccgtttctat ttcttcatct ggaacacctg cttcttttgc atgctggaag
    31201 aataagtcat caaaagaagg agtaacgatg acatcatcaa ctgcattttg gaacaatcca
    31261 aactcaaaag tcgtgcccgt acttatcgca gcacctcgta cgatattttc cactttttca
    31321 tatactttat ctaatgtttg ccgatttcct gcacgtaaat agaaacgtcc agcagcgtaa
    31381 tctggaatga cgttggcagc tactcctccg tctgtaatga tcccatggat attgacatct
    31441 tttggcaatt gcaagcgtag agcgttgatt ccattaaaca cttggatcaa tgcttccaaa
    31501 gcatttactc ctttttcagg tgctgctgct gcatgggaag cgacaccgta aaatttgatg
    31561 tcgactggat cattcgctaa agattctgtc gttttcccat aacgaaaagc tggatggacg
    31621 cataacgcta catcgacatc ctcaaaaaaa ccttctcgaa caaagctgcc tttagctgat
    31681 ccgttctcgc ccccttcttc ccctggggtg ccatatacac ggatttctcc accagtttca
    31741 tcaatgaatt gcttcactat gctggcggct aatacggagt aagtaccaaa caaattatgc
    31801 ccgcatgcat gaccaatacc tggtaaagca tcgaattctg ccaaaaaggc aattgtggga
    31861 ccgggtttat tggaaatata acgtgcatca aaggctgttc ggtgtccagc tacttctttt
    31921 tctacccaaa acctttcttt ttctaattgt ttaatcaacg ctgctgagga ataaaactca
    31981 tagttactta cttcaggatg ttcgtggata tctagagcaa gtgcttgata ttctggtaaa
    32041 cgatattgga ttagatcctt caattggttt gttgagattg ttgttgtcat aaaaaattcc
    32101 tcctatgcta gtcatttttc ttattttctc ccgaatgttt cccatgcagg cacgcttgat
    32161 ccttttgatg ttttctcgat tactttgact gtgtcttctg tttgataagc gtctactatt
    32221 ttttgatacg ttttattgtt ttcatcttct tttcttgcaa cgatgatatt cacataagga
    32281 cgtgcatggt catccactgg ttctaagaag atggcatcag aagcaggatt aaatccagca
    32341 tctacagcta caccgctatt gatcactgaa atgtctacat ctgacaaagc acgtgctgtt
    32401 tgggaagcat ctaattcaga aatgtctaga ttttttctat tctcggtaat atcagaaaca
    32461 gtgggcgctt gtccttttgc cttatccact ttgatcaatc cagcagtttg caataacaga
    32521 agagctctgc cgccatttgt aacatcatta gggattgcta cttttgctcc gtctttgata
    32581 tcagctacat ccttcaaagt gttagaataa attcccagcg gtgcattgac agtatttccg
    32641 atcgatacta aatcagttcc gtgttcttta ttgtaattgt ctaaaaagaa ttgatgttgg
    32701 aaagaattca aatcaatcga tccatctgct aaagccacat ttggttgatt gtaatctgta
    32761 aatttcacgt attccaaatt aatgttctcc tttttcaaac ggtctttcac gttgtcccat
    32821 acatctgtat cttctccaat aatccctagt tttacgatag tttctttttt gtcagttgta
    32881 tctgcttttc cttggctaca ccctgccaac caaataattg ctacaaatgt aatgactaca
    32941 aaacgaataa tttttttcat tttttctcct ccactttcaa tgtgtaattt ttttaatcag
    33001 ttgattactg ataaattgac ttaaaaaaac taagattaga atcaataatg tagccacaat
    33061 cgttacatct gtttggaatc gattataccc tctcgatata gcaagattcc ctaatccgcc
    33121 tgcacctact gctccagcca tggtagtcag tccgatcacg ttgatgatag tcaacgccga
    33181 gacccgaatg ataccaggca aaccttctaa caaatacaca cgtaaaatga taccgattgg
    33241 actggttccc atcgcttctg ccgcttcgat caccccagga tcaacttcta gcagtgcgtt
    33301 ctcgatttga cgagcaaaaa acggaacaac tccaaaaatc aatggcacta aagctgcttt
    33361 cgcaccaatc gtcgttccaa ctaagaaacg agtagtcaat gctaaaagag aaagcaagat
    33421 aatgaaaggg atggaacgaa tgatattgat cagcttatca agtatttcaa atagcagacg
    33481 gttagctagt aatccattag ggcgtgtcac tactaacgtc acacctaaga tgattccaat
    33541 catcccgcca atgaatgctg tccaaaaaga catatatagt gtttgcttcg tcgcttcgac
    33601 aaattcatcc ggaatcaaat aaacattggg caaatatgtt ttaaagaaat cagtcatttt
    33661 atcttcctcc ttcgatcact ttgatacgac tatctaaatc gtttttttgt aaaatattta
    33721 tttttacacc agatttgatc aaataatcca atgctttttt ccgctgtttg gtttctccag
    33781 ctaaagtaac gatcaaactc cctaacggcg tttcttgtaa taactctaca ttgccatata
    33841 aaatattcgc tgttacttga tatttgctgt acagatgagc gattagaggt tcgttcgtct
    33901 gatttccaat ataacttaat tccaccagcc atttattggc gatctgatcg gcaaaagcag
    33961 aatgggcaat gatcgtttct aatgcttggt caagatgagt agctgttcga ataaaatctt
    34021 tggtcaattc ttcttcagga ttgctgaaga tctggatgct gctacctttt tcaacgattc
    34081 ttccatcttc cataaccgct accttgttgc agatttcttt gacaacttgc atctcatgag
    34141 tgatcaggac gattgttaaa cctaactggc gattcaattt ttttaacaat gcaaggatct
    34201 gaagggtcgt tttcggatca agtgcactgg ttgcttcatc acataataat accttaggtt
    34261 cattcgccaa agctcgtgcg attgctactc gttgtttttg tccccccgac agttgtttag
    34321 gataagcaga tgctttttct tccaaaccga caagttccag tagttcattc actttttgac
    34381 gacgttcctg tttcgatttt ccagaatact ttaaagaaaa atcaacgttg tcaaaaatag
    34441 ttcggctttc cattagattg aaatgctgaa agatcatacc gatggccttt ctttcttttc
    34501 gcaattcttt gggagagagc tgactcagat tcgtctgatt gacaatgact tctcctgctg
    34561 ttggtttctg cagcaaattc atcacacgaa ctaacgtact tttccctgca cctgagtaac
    34621 cgacgattcc ataaacatcg cctttttcaa tcgttaaatc tacagaatct actgcctgta
    34681 tctctttttc tttttgtttg aaacatacag aaacatctcg caattcaatc attaaaattc
    34741 ctccttatct aaaaaaagcg tcctcaagaa aatgaaaatg tcattttctt gaggacgctt
    34801 ttttgcgtgt taccacctca tgttcaagct gtgttcacac acaactcctc ttcaagtacg
    34861 atcatactct agcaagatat cggtcgcagc cgtcatcgcc ttacatctat gctcgggatg
    34921 aattgctcga tggccatttt ccaatagtcg ttccttgctt cttctcagct accgaagctc
    34981 tctgtcaaag gttcttctat cgtacttacc atttcaatgc aaataatatt caaaaaaaaa
    35041 gtccttttac taacataaac tatgtcagta aaaggacgac gtatcgcggt accacctttt
    35101 ttcggaagtt tccttccctc aagcccaaca tctcaaaaag agagattggc gttcgtttta
    35161 acggtcgaac agttccgaat ttgcctactt atcgacaaat tattctccaa ggccattttc
    35221 agcttttctt acttgactct tctcaccagc cagagcctct ctgcaaagtt tccaaactta
    35281 ctttcctttt caacgaaatc gatattttgt tgttgggatt aatctacatg aggaaaactg
    35341 aaaagtcaag tacgaaattc aaattatttt ttctatattt atttttctgt ttttttattt
    35401 aacatttcaa taacagttcg aagatctttt ccttccgcaa gaagtttcaa tttttccttt
    35461 tcttcgacgg tataatttcg gacaaaactg gaatcaggta tcaaaacaac gcctaatcct
    35521 gctcgtgaag cagctgttgc tcctactaaa gaatcttcca ctgcaaaggc tgaactagct
    35581 tctaatcctg ttcgttccaa agctgtcaga taaggaatcg gtgaaggctt gttctccgac
    35641 acttcgtctc cgaatgtaaa cgtgtccaag taatcagcta atttaaagtg atccaagata
    35701 tctgttgctc ttttcgtaac agtggtcgta gcaatgccta gtttcatatg acgttctttt
    35761 aagtctgcca atatttcttt tgcatatggt tttaagtgga tggctccgct tgtcagctgt
    35821 tgataaaaat attcttcacg cttcgctcgg acttcctgta ctttttcagg gctacctgct
    35881 gctttcacta aaatatcaaa actttgtttc cagttttttc cgccccaagc gtcaacgatt
    35941 tcttttggga tatggatatc aaattgctga aatccccaaa gccagccatc acgatacgtt
    36001 ttttctgtgt caactaacaa accatctaaa tcaaatattg cacctttcat ctatacatta
    36061 atcctttcca aaacaataca catagtttag cacgactggt ccaaaaagaa cagaagcaaa
    36121 gaaccagcct tctcgtttat aagagttctt ggaatcgacg gatatagatt ttcttcacag
    36181 ctgtcacaag cgccagataa gcgatgactg tagcaaataa ccataaccag taattgccag
    36241 gaagtggcat caagcctaat tcagcaccaa aacttgtaaa aggaaggaca gaaccgacaa
    36301 taattcctaa tgtagtgata acaaacatgg caaatgttgc gttgctttgg ataaatggta
    36361 ctttcggtgt acgcagtgca tggagcacta aggtttgtgt ccatagtgat tcgacaaacc
    36421 atcctgcatg gaataatgcg atgaaaatca ctcgctgtga actatccaat gtatggaaac
    36481 ttccgcccac tacagcagga caaataacaa aatacatcaa caagtaagta gtaatatcaa
    36541 atatcgaact tgttggacca aagtaaacca tgaattcccc aatactagat gcattccatt
    36601 tttttggctc gtccaaatat tctttatcca tccgatccca tggcaaggaa atgcaggaaa
    36661 catcatagat cagattcaaa aacaaaagtt gtaaaggcag cattggtaaa aatggcagga
    36721 acgtacttgc aatcagcacg gaaaacatat tgccaaaatt agaacttgct gtagccttga
    36781 tatatttcat gatattccca aaagtttctc gtccagaaag aaggcctctt tccaaaatcg
    36841 tcaaatcttt ttctaaaagg atcacgtctg cagattcttt cgcaatatcc acagctgtat
    36901 ctacagaaat accgatatca gccactttca ttgcaggagc gtcattgata ccatctccta
    36961 aaaatccgac agtatgacca tttttacgta aagctgtcgt caaacgtgct ttttgctgag
    37021 gattcaattt tacgaatact tcatggtctt cagctacttg tgctaattca tgatcactca
    37081 tttccgagat tttctcaccg gtaaccaatt cattgatctc tagacctact tgacggcaca
    37141 ctgaacgtgt aactaattcg ttgtccccag tcagaacttt tacagctaca ccatgttctt
    37201 tcagagcttt caacgcttgt ttcgttgttt cttttggtgg atcaaggaaa gcaagataac
    37261 caatcaaaac catgtctgac tcatctttga ctgaaaattc tccaacgacg cttggattgg
    37321 tcttttgtgc gacaccaatc actcgtaagc catcttcatt aagtgactgt acttttgcaa
    37381 gtacggcttc ttttcgttct tttgttaatg gtgtaacagt accgccaaga tcaataaatt
    37441 tagaaatgtt cagcatttct tcaatcgcgc cttttgtgat catttgtgtt ttaccagctg
    37501 gatcagcaac cacaacactc atacgacgac gttcaaaatc aaaaggaatt tcatccactt
    37561 tttgataatt gatcttatct gtatctaaag tttctgttgc ttcatcaatg atagccacat
    37621 ctaacaaatt tttcagccct gtttgatagt agctatttag atacgcatgg cgcaaaacac
    37681 gatcatcttc atttccttca caatccatat gatattcaag aatgatttta tcttgtgtca
    37741 atgtccccgt tttatccgta cataacacat cgattgcacc aaagttctgg atggcattca
    37801 aatttttgat cactgttcct tttttggcca tcacacttgc tcctttgacc aaattcgtcg
    37861 taacgatcat tggaagcatt tcaggtgtta gaccaactgc tacagatagt ccaaaaagaa
    37921 aagcttctag ccaatcccct tttgtaagcc cattgatgac gataactgct ggagccatca
    37981 atgacataaa tttgatcagt agcatggatg ttttatggat gcctaattca aagcttgatt
    38041 gaattggttt ttcagatagc gtttctgcta catgaccaaa tagtgtttca tttccaacag
    38101 aaacaatgat tccttccgca cttccactga tgacattact tcccataaag acaagatttt
    38161 cgtaacttgt ttcgctttgc tgatcgtctt cacatacttt cgcatgcttt tctactggat
    38221 agctttcccc tgtcaatgct gcttgggaaa tgaataaatc ctttgcggac aataaacgca
    38281 tatccgctgg tatcatatcc cctgctgaaa gccgaacaag atctccacag acgatttctt
    38341 ccataggaat ctctgtgtac tttccattac gttttacagc tgccgttact ttcacaagtg
    38401 atttcagttt ttcagccgct tgattggaac gtacggactg aatcaacgtc attgtcccgc
    38461 tgacgataac catagtgaag ataatggcta caccgaaaag atctttatct ccaggagctg
    38521 ctaatacata ttccgtgatg aatgaaataa ttcctaatgc gatcaacact aatgtaaatg
    38581 gcgtgatgta tgctttcaat atttctacaa tgaatggtgt ttttttgcca tatgaaatcg
    38641 cattttcacc gtaattttct cttgaacgtt cgatttgttc ttctgtcaat ccatttgctg
    38701 atgcatggaa tagttcaaat aattcttgtt tcgtttttcc agcaaaagct gcatagattt
    38761 tttgtgtatc tttctttttt tccatctcaa tctctccttt gtgtccaaaa gacagctgtt
    38821 ctcgctgctt ttggtcttta gctattcatc ggatcgatag agaaaaaact gaaaggggct
    38881 gagaacagat agataatagc cctggccttt cctctagatc cttgtttttc gaatgactgt
    38941 catccatttg ttctcatctc ctttactaag taattttaat tcatgacttc caatcatttt
    39001 tcgaatagtt atttttccaa ttagaccacc tcctttttat ttttcaccga tggggatttg
    39061 cggcaacaaa aaaacctctc gccaaatact cagcgaaagg tttgaaaaag catattaaaa
    39121 aaagcttctc agatctcatc gttgagtttt gacactgtac aacgtaaaaa gcagatacct
    39181 gcttttagct accttatgaa aagccttatc tcgacaattc ctgttctacc cattggcgtc
    39241 tctcgacgtt tttgggcagt agcctatgtt tgtataggag cctcacctaa caaatcttat
    39301 tcggttttcc ttcgttaata taacaactaa aaaagaggat gtcaaatcaa ttttccatgt
    39361 ttttcatact ttttttaaaa tacacacgtt atattcctgt ctcacgcggt tttatttgtc
    39421 tcattttcca cctcccgcta taatagaaat atagtaggaa acgaggtgct gttatggaaa
    39481 gaacacttgt tattatcaag ccagacggcg tgagacgcca tttagtcgga agcatcatcc
    39541 aacgttttga agcaaaggga ctggccattg ctgaaatgaa gtttgaaact atgactcctg
    39601 aattagcaaa agaacattat cagcacttga ctgagcgcag tttttttgat gaactgatcg
    39661 attacatgac ttccggtcct gtcgtttatc ttgtgctggt cggcgaggaa gtgatcgata
    39721 tcgtccgtaa gatggttgga gcaacaaaag ctgccgatgc tgttccaggt acgatccgag
    39781 gagactacgc gctgccgggg actgaaaaca tcattcacgc ttctgattcc cgtgatgctg
    39841 cggtaaaaga gatcgcccgc tttttcccgc cattcgatat aaacaaaccg tctgaaaaag
    39901 cggcgaagtg atacaaattc taaactgctg gtctagttgc tgggttcagc aatggccagc
    39961 agttttttct ctaaggacct ccgttgattc tttcctaaca tatattcgag cactgtgaag
    40021 aattcttggt agtctttcgt cttttcttcc cctaaagcag cagaaatcaa tctttgataa
    40081 taatctgcca tcaatgtttc atagatcact gtacttcttt ctttatgtgc taagctccgt
    40141 ttcatatctt cattcacact acgtgccttt ttcaaccgac cattttctat ataaaacgaa
    40201 gtcaggttca ttaaaaaagt aaagagatac tgctcatgat tgttcaactg tgctaatcgt
    40261 cgcattcttt ttttgatttt caaataattg ttctcaatga accgatcact gaatatccaa
    40321 ggaaaatcag ctagaataga tagttcaaat tgaccatagt ctggtatctg catcaagtat
    40381 tcttcaatga ttcggcattc ataacgtaaa aattcaaatt ctgtctcaga tacttctgtt
    40441 ttttcgacca tgtgaagatt caaaaccaaa taatacagat cttctgtttt cttaaaatgc
    40501 ctacggttat tgctgatctc tttataagga tcaaagctgt cttcttgtac ccgatctagc
    40561 attcgtcttt ttttctttaa ctttattggt tcatccagct ggtttcgcat ccattcatat
    40621 tctgcaagtg tcatgttcat tcgctctaaa aatgatctca atgttacaaa tgacattttt
    40681 ttcactgaat ttgtctcaat cccgactaga gaagtcttaa aacagattcc ttccgctaat
    40741 tgggactgtg tcaacttctt gttgactcga agtttcttga tcaattcacc atcatacata
    40801 atagttctcc tttttaattt aaatttttcc acattcaata aatagatacg ttaaaaagga
    40861 aaaatacacg aaaaaaataa aaaaatgttt ttttcataaa aaataccaac cttcaagcat
    40921 tagaatgttg tctaactact gaaggttggt ttgattgttt attgattatt tttttgctgt
    40981 tcaacgtctg ttcttgttac tggatataag ttggttgttg ttttattacc attttttgaa
    41041 aagacacttg tcgaatgttc gcctttttgc ttttcgattg cttcattacg ttccgcttca
    41101 tttttatagt catatttttt cggatcaatc gtctcaaatc cgtttggcac ataaaaccgc
    41161 aagagatttt cctgattcag cttatctgaa agacttaacg cagtcttgac tttttcctga
    41221 tctttggaga tttcctcatc gattcctgct gctttcgtat cgatgatttc tcctgtctga
    41281 ttattgtaag cttttcctcc taggacggtg tatttgggtg tcacaaagtt cccatttcga
    41341 aaagcaacta cttgatcatg ctgagaagat aataagtctg taccaaaatg aagataatct
    41401 ttatcatcga ttcccaatag atgtagtaat gtcggcaaaa catcgatttc tccaccgtat
    41461 tcatgattga ctgtcccttt agtataacca ggcatatgga tcatcaatgg cactcgttgc
    41521 atctgtgcgt tgtcaaattc atcccatgta tctggatctt taccaagagc gcttgctaaa
    41581 tctttattat ctgtattcga gatcccaaaa tgatccccgt aaatcacaaa gattgaattt
    41641 tggtaaatac cagatttttt caaatacgta aaaaattgtt ccaatgattg atccagataa
    41701 tgtgccgttc gaacataatt atctactgtg ctattgccag tattcaaaga tggaaagtcg
    41761 aaattcttgt catccgtata ataaggcgtg tggttggtta cggaaagaaa tttcgtgtaa
    41821 aacggctgtt gtagatgctc aagatattgg gcagattctc tgaataaatc tttatctaat
    41881 atcccataac ctaacgtttc gtcggattca tcaaaataag atcgatcaaa gaaattgtca
    41941 taacctaagt ttttatatac atgatctcta ttccaaaaac tagctacatt cccatggaaa
    42001 acagcgcttg tatagccttg cttttgtcca aggatcgctg gtgcggcttg aaagacattg
    42061 tctgacccaa gttctgtgaa taatgagcct tgaggtaaac caaatgtgcc agtttccaac
    42121 atattttcag catcacttgt ctttccctgt cctacttcat gaaagaaatt gtcaaagctg
    42181 atcgttgctt gattctggaa aatactattt aaaaacggtg tgacttcttg tccgtctacc
    42241 ttcatattaa tcaaaaactg ctggaaactt tctaaatgga gaacgatcac atttttccct
    42301 ttagcgatcc caaatgtttc gggatttggc tctgcataat gtttctttgt ataatctaaa
    42361 acttcatcaa tcccattact gcttgcatgc gcgcgaacac tgctagtcat tcctgttttg
    42421 attccgtcat aaatagtata cgcatcgatt cctagatatt tgacaatata tgaacggtca
    42481 aatgttcgct gcagcaattg tggacggttg gcttcgctca gcgctagatt tcctgaaaaa
    42541 ataagccctg aaagacaggt aacagcgata gccatcggtt tgccgacagg gttgcttttg
    42601 ataggtattt tcttgacctt caaccaaacc agtagcccaa tgaacacagc gatatccagc
    42661 cagtagatga catcatgggg cttcatcaaa gaaaaggagc ttcctgacaa tccttgcgac
    42721 actttcgaaa agcctaatac agatttgact gtaatgaaat ctgtgaattc ccgataaaaa
    42781 ataatattca agtacaacaa aagcgtattt aaaatatcaa tgatcaaaag aatagctaac
    42841 gctggctttg gctttttaat atacaaagaa aggccaaaaa acaataaggt agtcgcaatt
    42901 gggttgatcc acaagataaa gtactggatc gtaccttcca cacctaatga aaaatctgca
    42961 tagtaggcaa tgatcgtctt cagccagaaa aagaaaacag ctgtcaaaac gattgctaaa
    43021 cgattttggc acaatttttt aagttgtgtc acgtatatca cttctctcta ttcacatttt
    43081 ttcttatttt gctcctgaaa aatgaaaatt tcctaaaaaa gttgttatct taagattttt
    43141 ttattctttc taaagattat tccgtccact tttcacagca taaaaccgct gcgatgtaag
    43201 cgattaaact aatgaagaga taaaagtctt gtggtgaccg cttcactcca cggtattcaa
    43261 aagccagttt ctcggtattc gtacaaaaaa agaggctgtg acaaaattct tgtcgcagct
    43321 ccttatttct tgatgcagga cagctttttc acaacttctt ccttaaacgg tgttctcctg
    43381 ccaactcctc ggccacaagt cacaatattt ataaaacatg agaagctgtt ttttgaaatt
    43441 gttccttgtg gctcagagtt aaacggcagg ctcaagacct cttgatacac ggtgttcaat
    43501 cagctgaccc tcggtattag attatgtcag gacgctaatt ttacaacctc ttggtaaacg
    43561 gtgttcaaaa gcttgcatct gcggtaataa gcccaaccaa acgaaaaata taaggagcta
    43621 ttttttgaaa ttgctccttg tggctcagag ttaaacggca ggctcaagac ctcttgata
//
LOCUS       DACGLW010000014        41655 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.014, whole genome shotgun sequence.
ACCESSION   DACGLW010000014 DACGLW010000000
VERSION     DACGLW010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41655
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.014"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            104..634
                     /locus_tag="IXT53_000834"
     CDS             104..634
                     /locus_tag="IXT53_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010781150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HAQ4904682.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELW"
     gene            766..3126
                     /locus_tag="IXT53_000835"
     CDS             766..3126
                     /locus_tag="IXT53_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HAQ4904683.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            3213..3527
                     /gene="trxA"
                     /locus_tag="IXT53_000836"
     CDS             3213..3527
                     /gene="trxA"
                     /locus_tag="IXT53_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HAQ4904684.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            3709..5487
                     /gene="uvrC"
                     /locus_tag="IXT53_000837"
     CDS             3709..5487
                     /gene="uvrC"
                     /locus_tag="IXT53_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HAQ4904685.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            5632..6531
                     /locus_tag="IXT53_000838"
     CDS             5632..6531
                     /locus_tag="IXT53_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4904686.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            6524..7786
                     /locus_tag="IXT53_000839"
     CDS             6524..7786
                     /locus_tag="IXT53_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ4904687.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            7949..9847
                     /locus_tag="IXT53_000840"
     CDS             7949..9847
                     /locus_tag="IXT53_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HAQ4904688.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(10146..10865)
                     /locus_tag="IXT53_000841"
     CDS             complement(10146..10865)
                     /locus_tag="IXT53_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HAQ4904689.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     gene            11202..11534
                     /locus_tag="IXT53_000842"
     CDS             11202..11534
                     /locus_tag="IXT53_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4904690.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            11521..13524
                     /locus_tag="IXT53_000843"
     CDS             11521..13524
                     /locus_tag="IXT53_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HAQ4904691.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            13543..14013
                     /locus_tag="IXT53_000844"
     CDS             13543..14013
                     /locus_tag="IXT53_000844"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HAQ4904692.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            14034..14618
                     /locus_tag="IXT53_000845"
     CDS             14034..14618
                     /locus_tag="IXT53_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HAQ4904693.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            14633..15619
                     /locus_tag="IXT53_000846"
     CDS             14633..15619
                     /locus_tag="IXT53_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HAQ4904694.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            15609..15920
                     /locus_tag="IXT53_000847"
     CDS             15609..15920
                     /locus_tag="IXT53_000847"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HAQ4904695.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            15942..17723
                     /locus_tag="IXT53_000848"
     CDS             15942..17723
                     /locus_tag="IXT53_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HAQ4904696.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            17716..19092
                     /locus_tag="IXT53_000849"
     CDS             17716..19092
                     /locus_tag="IXT53_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HAQ4904697.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            19099..19734
                     /locus_tag="IXT53_000850"
     CDS             19099..19734
                     /locus_tag="IXT53_000850"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HAQ4904698.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            19764..21119
                     /locus_tag="IXT53_000851"
     CDS             19764..21119
                     /locus_tag="IXT53_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HAQ4904699.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(21311..22705)
                     /locus_tag="IXT53_000852"
     CDS             complement(21311..22705)
                     /locus_tag="IXT53_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAQ4904700.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            22797..23768
                     /locus_tag="IXT53_000853"
     CDS             22797..23768
                     /locus_tag="IXT53_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HAQ4904701.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            23765..24547
                     /locus_tag="IXT53_000854"
     CDS             23765..24547
                     /locus_tag="IXT53_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HAQ4904702.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(24716..25087)
                     /locus_tag="IXT53_000855"
     CDS             complement(24716..25087)
                     /locus_tag="IXT53_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAQ4904703.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            25221..25664
                     /locus_tag="IXT53_000856"
     CDS             25221..25664
                     /locus_tag="IXT53_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAQ4904704.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(25707..26516)
                     /gene="yidA"
                     /locus_tag="IXT53_000857"
     CDS             complement(25707..26516)
                     /gene="yidA"
                     /locus_tag="IXT53_000857"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HAQ4904705.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(26535..27902)
                     /locus_tag="IXT53_000858"
     CDS             complement(26535..27902)
                     /locus_tag="IXT53_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ4904706.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(27886..28716)
                     /locus_tag="IXT53_000859"
     CDS             complement(27886..28716)
                     /locus_tag="IXT53_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HAQ4904707.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            28879..29316
                     /locus_tag="IXT53_000860"
     CDS             28879..29316
                     /locus_tag="IXT53_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HAQ4904708.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            29392..30012
                     /locus_tag="IXT53_000861"
     CDS             29392..30012
                     /locus_tag="IXT53_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HAQ4904709.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            30201..30707
                     /locus_tag="IXT53_000862"
     CDS             30201..30707
                     /locus_tag="IXT53_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HAQ4904710.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            30724..31623
                     /locus_tag="IXT53_000863"
     CDS             30724..31623
                     /locus_tag="IXT53_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 and DUF4163 domain-containing protein"
                     /protein_id="HAQ4904711.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            31710..32594
                     /locus_tag="IXT53_000864"
     CDS             31710..32594
                     /locus_tag="IXT53_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HAQ4904712.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNYTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(32786..32857)
                     /locus_tag="IXT53_000865"
     tRNA            complement(32786..32857)
                     /locus_tag="IXT53_000865"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(32822..32824),aa:Arg,seq:ccg)
     gene            32917..34251
                     /gene="rpoN"
                     /locus_tag="IXT53_000866"
     CDS             32917..34251
                     /gene="rpoN"
                     /locus_tag="IXT53_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HAQ4904713.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            34454..35491
                     /locus_tag="IXT53_000867"
     CDS             34454..35491
                     /locus_tag="IXT53_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SorC family transcriptional regulator"
                     /protein_id="HAQ4904714.1"
                     /translation="MLDEIKMIESVAPDIIEVLQERYKILRNIYWMQPIGRRSLSESM
                     GITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSLENFMDQLLGTHQTEQQLA
                     EYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEGENIIAVMGGTTMAVVAEQ
                     LSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKANGNHRALYVPEQLSLATYN
                     SLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEKELVMLKQANAVAESFGYF
                     FNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAIRAYMKNAPKQTWLITDEA
                     AANEILKGVTL"
     gene            35532..36533
                     /gene="gap"
                     /locus_tag="IXT53_000868"
     CDS             35532..36533
                     /gene="gap"
                     /locus_tag="IXT53_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAQ4904715.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            36643..37833
                     /locus_tag="IXT53_000869"
     CDS             36643..37833
                     /locus_tag="IXT53_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HAQ4904716.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            37937..38692
                     /locus_tag="IXT53_000870"
     CDS             37937..38692
                     /locus_tag="IXT53_000870"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HAQ4904717.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            38808..40106
                     /gene="eno"
                     /locus_tag="IXT53_000871"
     CDS             38808..40106
                     /gene="eno"
                     /locus_tag="IXT53_000871"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HAQ4904718.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            40505..41644
                     /locus_tag="IXT53_000872"
     CDS             40505..41644
                     /locus_tag="IXT53_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HAQ4904719.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT        13825 a         7285 c         8921 g        11624 t
ORIGIN      
        1 aaacgagaaa cgaaaaggac aaattaaaca aaaatcttct aattaagatc cttttatcaa
       61 aataagaaaa atttaaaaaa gttagaagag aaaggaacgt agcatgctga gtttgctgat
      121 tttattttta ttgctaattg cgtttttttc tggagcacgt cgcgggtttg ctttgcaaat
      181 catattcgcg attggatatg tattgtcatt tattgctgcc cagcattttt acaaaccatt
      241 agcaagtcat ttggaattat acattccata cccagctgtc accccaacat caaagttagc
      301 attttttgat caggtatttg cttttcacct agatgaagct ttttatgcag gaactgcttt
      361 tctgattatt ttgctaattg gctggctgct tacccgattt gtgggggttt ttgtccatgg
      421 attgacgtat gtgccgatct tgagacaggt agactggatt gcaggaggca tcctgtcact
      481 aatcatggct tacgttacta tatttttgat tttgcagtta cttgcctttg ttccattaga
      541 tgtggtccaa aatcaattcg gcgagcatag tttcgctcgt tttatcgttg agagaacgcc
      601 atttcttacc aacaagatgt atgagctttg gtaacgaata ttttgaatta aggaaaatag
      661 tgctggaaaa tatttcgttt tttgtacaga gtagtttatt taagaaggct gcgtagcgta
      721 tgtccggcac agctcttttt tcatagtgaa agataaggtg ataaaatgaa taaccgaatc
      781 cttgaaacat tggaatttga aaaagtcaaa cagatggtca gacaatttgt agtgactgca
      841 caaggaaaag aagaactggc agaattggta ccagtgagtg agaaacaaac gatcacaaac
      901 tggcttcaag aaactgagga tggattgaaa gtgcaacgtc tgcgaggcgg gatccctata
      961 cctaaacttg aaaatatccg tccccatatg aaacggatcg agatcggagc agacttgaat
     1021 ggagtggagc ttgcacaagt ttccagagta ttgtctacga caagcgaatt gaatcgtttc
     1081 attgatgatc tttctgacag tgaaatcgaa tttgcacgtt tgtatatgtg ggcagatcag
     1141 ttagtcacga tccctgtttt gagccgccgt ttaaaggaag caatcgatga agacggacgc
     1201 gtgactgatg acgcttctcc tgagttaaag agcataagac agaacattcg gcgaagtgaa
     1261 caagctgttc gtgaacagtt agatgggatc gtccgtggga aaaatgcaaa gtatttaagt
     1321 gatgcgatca tcaccatgcg aaatgaccgc tacgttatcc cagtcaaaca agaataccgc
     1381 ggtgtatttg gcggtgtagt ccacgatcaa agtgcttcag gacagacttt atttattgaa
     1441 ccaaaacaag tggtggattt gaataatcgt ttgcgtcaat accaaatcgc tgaacgaaat
     1501 gagattcaac gtattttaag tgaactttct gctgaacttg ttccccatcg tcaagaaatc
     1561 atccacaatg cttatgtgat tggtaaaatg gatctgatga atgcaaaagc acgatttggt
     1621 aaagaagtaa aagccatcgt gccgggaatc agtgaagaca atcatgtcgt cttgaaacaa
     1681 gcaagacatc cactgatcga tcaggaaaaa gtagtttcta atgacatcac aatcggaaaa
     1741 gactatcaag ccatcgtcat tacaggacct aacacaggtg ggaaaacgat cacgttgaag
     1801 actttaggtc tgttacaact gatggggcaa gcaggattac cgattcctgc aggtgaagaa
     1861 agtcaaatcg gtatttttga ggaagtattt gcggatatag gagacgaaca gtcgattgag
     1921 caaagtctat cgaccttttc ttctcatatg acgaacactg tagatatttt atcaaaagtc
     1981 aatgaaaaaa gtctggtatt atttgacgag ctcggtgcag gaacagatcc gcaagaaggt
     2041 gctgctctag caatcgcaat cttagatgat cttggtaaaa agtctgctta tgtgatggca
     2101 acaacgcatt atcctgaatt gaaagtttat ggatacaatc gggcaaatac gattaatgcc
     2161 agcatggagt tcgatgtgga tacactaagt ccaacgtatc gtttgctgat tggcgttcct
     2221 ggacgaagca atgcgtttga aatctctagc cgattaggtc ttgatacaga agttattgat
     2281 gaagcgaagc agttgatgaa tgatgagagc caagatttaa acgaaatgat cacagattta
     2341 gagaatcgcc ggaaaatggc agagaccgaa tatctggaaa tgcgtcattt tgtttctgag
     2401 gcacaagaac ttcacgatga tttgaaagaa gcttacagct atttctttga agaacgtgaa
     2461 aaagaaatgg aaaaagcaaa gaaaaaagca aacgaagtcg tgtcagaggc ggaagaaaaa
     2521 gcagaaaaaa tcattgctga tattcgtaaa atgcaacagc agattgggca aggaaatgtg
     2581 aaagagcatc agttgatcga tgcaaagacc caattagcaa atctacatca agaagaaact
     2641 ttgaagaaaa ataaagtatt gaagaaggcg aaagaacaaa aaacgttgaa accgggagac
     2701 gaagtgttgg ttacgactta cggccaaaga gggacgcttt tacgtaaaaa cgggaatcag
     2761 tggcaagtag aaatcggtat attaaaaatg aatgtgtccg aagacgagct gacgcctgtt
     2821 gctcctcaaa aagagccgac acaacgagtt atccatgctg tacgatctga atctagtagt
     2881 cacgtgccga atcaattgga ccttcgagga aaacgatacg aagaagcgct aagtgaggta
     2941 gatcaatatt tggattctgc cattctagct ggttatcctc aagtgactat tgtccacgga
     3001 aaaggaacag gcgcacttcg caagggaatt actgattatc ttaaaaacca tcggagcgtc
     3061 aaaagctttg agtttgcacc ggcaaaccaa ggcggaaatg gtgcgacaat tgttaaattt
     3121 aagtaaagct tactaaaggt aagcagatgg ttcgctatcc aagtatagaa atgctatact
     3181 aatcatagat tttaaaaatg gaggtctttg atatgtcaca agtaattaca gataagacat
     3241 ttaatgaaga aacagataaa ggacttgtat tgattgattt ttgggcaact tggtgtggtc
     3301 catgtcgtat gcaagcaccg attttggatc agttggaaca agaatatgat gaagaagaat
     3361 tcagaattgc taaaatggat gtggatgaaa atccagaaac tcctcaacaa ttcggtatca
     3421 tgagcattcc aacattaatg ttgaaaaaag atggtcaagt agtagaaaaa gctgttggtg
     3481 ttcattcaaa agaacaacta cgtcaaatga tcgatcaata tttataaaaa cttacagcaa
     3541 aaataagctg agggtgggac tgaatccagt tccgtcctcg gtttttttgc ggttgagtta
     3601 aggttattca aattaatatt ttttgagtat aattacgttg tcaggctgga ttgttttttt
     3661 gacagacatg ctataataac tttaattgaa tatgaagggg agtaccagat gaacgaacga
     3721 atcaaaaaca aattagcttt acttcctgat cagcccggct gttacttgat gaaagataaa
     3781 aatggaacga tcatttatgt cggcaaagcc aaaatattaa aaaatcgtgt ccgctcatat
     3841 tttactggca gccataatac gaaaacggaa cggcttgtta gtgaaatcga agactttgaa
     3901 tacatcgtta cagaatcgaa tatcgaagca ttgctcttag agatcaatct gataaagaaa
     3961 aatgatccta aatacaacat tatgttaaag gatgataaaa cctatccttt ccttaagata
     4021 acgaatgaaa aatatccccg tttggtgatt actcgtaaag tactaaaaga caaagcactg
     4081 tattttggac catatcccga tgtcggtgcg gcgaatgaga caaagaagat cctcgatcga
     4141 ttatttcctc tgagaaaatg caaacctagt cagacgaagg agccttgtct ttactatcat
     4201 ttgggacaat gtctgtgtcc ttattatttc gacgtagatc cgaaagtcta tacgaaaatc
     4261 gttgatgaag tgaaaaagtt tttaaacgga ggacacgaaa aaatcgagtc agagatccat
     4321 cagaaaatgg aaaaagctgc tgaaaacatg gagtttgaaa aagctgcaga ataccgagac
     4381 cagatccgtg ccattgagac aatcatgact cgccaaaaaa tgacaaatac tgatttactg
     4441 gatcgagatg tatttggtta tgcgattgac aaaggatgga tgtgtgtcca agtgtttttc
     4501 gtaagacaag gaaagctaat tgaacgagat gtttcaatgt ttcctttcta taacgaagca
     4561 gaagaagatt tcttgactta cctcggtcag ttttatcagg aaaatgagca tttcattcct
     4621 aaagaagtct tgattcctga tacgatcgac aagcccagtg tagaagcttt gttagctacg
     4681 aaagtgctgc aaccaaaacg aggagaaaag aaaaaattag ttgaattggc gaataagaat
     4741 gcctcagttg ctttgaatga acggtttgat ctgattgcca gaaaacagga acggacgatc
     4801 ggagcagttg aacgtttagg aaacgcgatg aatatcccga ccccaatacg tatcgagtct
     4861 tttgataact ctaatatcat ggggacgaat cctgtggctg cgatggtcgt attcattgat
     4921 ggaaaaccgg caagaaaaga atatcggaaa tataaaataa aaacagtcaa cggaccggat
     4981 gattatgcat caatgagaga agtcatctat cgcagatatt cacgagtgat tcgcgaaggg
     5041 ttgcctttac ctgatttgat cttgatcgat ggaggaaaag gacaagtaga tgtggcaaaa
     5101 gatgtcttag aaaatcaatt aggaatcgat attcctattg ctggcatggc aaaaaacgat
     5161 aaacataaaa caaatgagtt gctattcggt atagatttgg ctgtagtacc gcttgagcga
     5221 aattcaccag agtttttctt actccagcga atccaagatg aagtccatcg attcgcaatc
     5281 actttccatc ggcagctacg cagtaaaaac agttttgctt cccgattaga tgaaattgaa
     5341 gggttaggac caaaacgaaa aaaaatgctg cttaaagagt tcaaatcgtt gaaaaatatc
     5401 acagcagcca cgacagaaga attacaagaa atcggactgc cgaaaaatgt cgcacaaaac
     5461 gtatttgata aattacatca ggaatgagga aagggcactt tacaaagaaa gtaaagtgtc
     5521 ctatcatttt atgtacaaca aaaagagcgt ttacgttttg ttggtaaata ataaatcgct
     5581 tacgtctggt gtctaaggat atatgtgatt tcttgaagaa ggagtgaaga aatgttagaa
     5641 gtcaaggatc ttgttaaaac ttttgggaac tacacagctg tcgatcatgt ctctttccaa
     5701 atccccgatg gaaaaatcat ggggctaatt ggccaaaacg gggctgggaa gacaacgact
     5761 tttcgtttga tcttggattt tttgacacaa gaccaaggag aagtgttatg gaatggccat
     5821 catcttgggg agaaagatta tgatgtcatc ggttatcttc cggaggaacg gggactttat
     5881 ccgaaagtaa cgattcaaga ccaactgctt tattttgcag aacttagagg gaaatcaaga
     5941 aaagagattg aaccaaaaat cgatttttgg atggaaaaat ttcaggtaaa agggaaaaag
     6001 acggataaag taaaatcttt gtctaaagga aatcagcaaa aagtccagct gatcgccacg
     6061 ttaatccatg aacctaaatt gatcattttg gatgagccat tcagcgggtt agatccggta
     6121 aatgctgaat tgctaaaaga tggaatattg gaattgaaaa aacatggttc atgtgtcatt
     6181 ttttctagcc acaatatgga taatgtagaa aaaatctgcg atcatttgat catgttgcga
     6241 aacggaaaaa tggttttgaa tggaaaagta catgagattc gtgaatcatt tggccgtacg
     6301 aagttattct tggaatctgg tttaagccaa caggaagtag aagaaatcga aggcgtgaaa
     6361 aaagtgatcc ttcatgaaga caatacgtta gagattacct tagaagatcc tgaagctggg
     6421 aaagaaatct ttactcgtgc gactcagttc ggctacattc ctatgtttaa ccagcaaccg
     6481 ccaactttgg aagagatttt caaattgaaa gcaggtgaaa tcgatgcgta agttttggat
     6541 tattaccaaa gatgtctatt taaaaaatgt taagtcgatt tctttcttga tcatgatatt
     6601 agtccctttt atcatgctgg gtatcattta tgtagcagga aactttgctc aacaaaatag
     6661 tgatgtcaat aaaatcggcg tattggctga taatgagcaa atcagtcagc aaatgagcca
     6721 agtcaaaaca gatgattttg agttcaaagc agttgattct gaagaaaaag cacaaaaaga
     6781 gctttcggaa gaaaaaatcg atgcgtattt agtagtctca actgagaatg ataagataaa
     6841 tgggatgctt tatagcgaaa attcattagg acaaagtaca caactactga tacaacaaca
     6901 actgactggc ttgcaatcga tgcttcgagc aagtcaatta ggcatttcac cagaagaagt
     6961 agctagcttg agccaacaag caagtttgac tcgacaaaaa gtcaattttg attcaaaagg
     7021 aaaaatgaca gttggagaag ataactcgac agtccaatat gtcgtaagtt atgtagcaac
     7081 gattgtcttg ttcattatta ttttgacgta tgcgcagatc attgcacaag agatcgcttc
     7141 tgaaaaaggc acgcggatca tggaagtgat cctgtccagt acgactgcac aaaagcattt
     7201 ttacggaaaa ctaacaggtg ttttacttgt tgcattaacg caaatggcat tatacggtat
     7261 tatttttggt ataggataca gtcaattcaa agatatggaa atggtcaaaa atatattaga
     7321 cggtatctca ttggataact tgattggacc attcttgtgg tattctcttc tattcatgat
     7381 ctttggcatt ttgatttttt ctgttttagc agctttatgt ggctctttgg tcaacaaagc
     7441 agaagataca gcaaaagcaa ttttgccagt gacttatcta tctctaggcg gatatatgct
     7501 tggattgata ctaggagctt cagatccaaa caatatcgtt attcggatca cttcgtatat
     7561 tccattctta tcttcatata ttatgccggt tcgcttagca aatgagacag tcggtatggg
     7621 tggagcattg atttccttgt tggtacttgt agccgtaaca ttcttattga tgatcttgtc
     7681 tgcaaacatg tacaaatcga atgtactcgt gtatagcgaa ggcggattat ggagttcatt
     7741 gaaacaatcg atttcaatta tgagaaatga acgaaaaaaa gcataaaata tagaaaaata
     7801 tagaaggaag ctggacattt attgttccca gcttcttttt tgtatagacc tatttctatt
     7861 tttttattat attggatata tatattcatt gaaaatagag cataacaact ttataatgtt
     7921 gagtatacca atttttggag gcgttttaat ggaccgctat ttagatttac tttcagagaa
     7981 gttccctaca acggaagatg tcatcacgga aatcatcaat ctcgaagcaa ttttgcagct
     8041 tccaaaagga acagagttat ttttgagcga tatacatggc gaatttcctg cttttgatca
     8101 tattctgcgt atcggttcag gaaatcttaa agaaaagatc aaagaccttt tctctgaaca
     8161 aatgacggaa acggagatca gccaattcac tatttttacg gcttatccag aatatgtgtt
     8221 gacgactgat tggtacaaaa aacaagataa atcccagctg atccatcgtt tgatcgatct
     8281 tttacggttt acgacagtca aatacactcg atcaaaagtt cgaaaaggat tgccgaaaga
     8341 atatagctat atcattgagg aactcttgta tatcgatgat cgtatcaacg ggaaaaaaga
     8401 ctatgtaaaa aaaatcattg aacaacttgt cttgatgaag gaagaagaaa aatttttaac
     8461 aaaacttgcg cagacgatcc aaaaatctgt catcgatcat ttgcatatcg tgggtgatat
     8521 ttttgatcga ggaactcaag cagccaaagt aatggatcga atcatggatt tttcctctgt
     8581 agatatccag tgggggaatc atgatatttt gtggattggt gcgtattgcg gctctgaggc
     8641 atgtttattg acgcttttac ggattgccgc acgctacaac tatttatttg aattagagaa
     8701 agaatatgga ttaaatttac gtccattatt ttcttttgcc catcaaacct atcagaagaa
     8761 tccagtattt acccctaaaa acaggaaaga tctatctgaa agagagcaag aagaattaga
     8821 aaagatccat caagcacttt ctatcctcca attcaaatca gaacatcagt tgttgaatcg
     8881 acgacctgaa tttaaaatgg acgatcgtaa attgttggaa aaaatcaact atgaggaatc
     8941 aacgatcgtt ttagaaggtc agctctatga gttgaaaggg acttgttttc aaacgatcca
     9001 gccagatgat cctaaaaaat tcttggaaga ggaacaaaaa gtgatcgata gtttgatgta
     9061 ttcattccag cactcgttaa gaatgcaaaa acacatgacg tttttgatcg aaaaaggggg
     9121 aatgtatctc acgaacaatc agcatgtact gttccatggc tgtatacctg tagatgaaaa
     9181 ggggcagctc ttagcatttg aattagacca atcttatcgc ggaaaagaac tgctgggatt
     9241 ttttgaaaaa cagataactg aaagcgcaaa agatttgact gcaaatgaag atttggcgac
     9301 cgatttgatt tggtatgcgt ggagcgggcc attttcgcct ttattcggca agagtaaaat
     9361 ggcaactttt gagcgctatt ttctcacaga gcctcatacg cacgtcgaaa aaagcaatcc
     9421 ctattatcac ttgcgagatg aagagtggtt cagtgaaaaa attttggcag aattcgatgc
     9481 aaaagaagaa ggttcagcaa tcatcaatgg acacacgcca gtaaaagtaa aaaaaggaga
     9541 gtcaccgatc aaagcaaacg gaaaagcatt tgttattgac ggaggtctat ccaaagctta
     9601 tcaaaaaaca accgggattg ctggctactc attactatgc aattcttacg gtttccaaat
     9661 cgtgacacat tatccgttct tgcctattga acaacaattt gcaaaaaaat ctgatcaaac
     9721 gaatgtaaaa aaagtgatcg agcagcagct cccacgcaaa cttaatcgga atacgacaaa
     9781 aggaatggaa ttacagcaac agattactca actccaaagg ctgttggatt atcgaaagga
     9841 cgattgagaa aaatgctagc caacattgct caatagacta aaaagcgact gcaacaaaag
     9901 gcgtgtcgca gtctccttcg gaattagctc aaaaaacggt aaaatattag aagctatttt
     9961 tctgtttatt tctctaatac tcaggtcagg gcgctgattt cacgacctct tccttaaacg
    10021 gtgttctcct gccaactcct cggtcacaag tcacaatatc caacaaacat gagaagctgt
    10081 tttttgaaat tgctccttgt gactcagagt tgaacggcag gctcacgacc taatcagaaa
    10141 aacgattaaa attcattcac aaagaaatcg aataaacgct gttcagatga atctacacga
    10201 tgagaaagtt ccggatgcca ttggacacca aggattttgt gcatattatc tttactttgg
    10261 actgcttcaa tgagtccatc gttggaaaag gcgatggctt ttaacaaagg agaaagatct
    10321 ttgactgcct gatgatgata ggaattcaca aagtatgaat cagggagaag gtcacttaat
    10381 aaactgtccg aaacgatttt gacttcgtga gttgcaaact gcggggcagt gggctgttgt
    10441 tcgtgtttta cggaccattt tggatataat gaaagatctt gataaagtgt tccctccaac
    10501 acgacattca atagttgaag tcctcggcag actgcaaaaa tcggttttct ttgtttcaat
    10561 gcttccaaga tcaatgcctg ttcgaattgg tctcgttgaa tatttgtttc cgtcagtttt
    10621 ggatgagggt cttccatgta aagttgcgga ctaacatcct gaccgcctgc aagaatgagt
    10681 ttgtcgattt tctcaattag ttgaggcgct gaatctggag acatgactgg cagaataaga
    10741 ggtaatcctt gagcatctaa aactgcatcc acaaatccct gcggtgtata gcttacttga
    10801 tttccttgga aagtatctgt cgctcgaatc agttgattcc cggcaatgcc gatgattggt
    10861 ttcattttta ttccccctaa agtagactaa ttgtaacaag gatttaaaaa aatttaaact
    10921 atgtaagact tgcccctata cttatctgtt cttgctgtgt tactatctgt aaagagaatt
    10981 ttcgtgtgaa attcatgacg atagttgaaa atattgctta atttgttata ataaaaagta
    11041 agcagaaata aaaaggttgg gcgtaagctt caaggatcta gttcttcaag gggtgagaag
    11101 gacattttat tgaaggggcc tgcctatact aagttatctt tagaggtaca tactttcaat
    11161 gaaaaaagaa agcatgtacc tcttttttaa atcgaaagga agtgaagcaa gtgttagaag
    11221 ctttggaaca aatccgaaaa gcagaagaag aaaatgagaa gcgtgctcga accctacggc
    11281 agcagttgaa gacctatgca gaagaaaaag agcaagaatt ggagataatc caaacgaaca
    11341 gtcggcaaca gctgaaacag ttggtagaag aaaaagaaca attcgaaaag caagaattag
    11401 aaaaagaaaa aatgaaattg aaagcagaag cagacaaggt gcaagcaact ttgaaagacc
    11461 aatatcaaaa gcatcagagt caagcaatcg gtgcaatcat tgaaagggtg aaagaaactt
    11521 atggccgtca ctaagatgga aaaagtgacc ctcatttcag ataaaagaaa tcaggagagg
    11581 attttacaag cagtacaagg aatccaaaat gttgaaatcc gggatctgta tcaaaataga
    11641 acaaacaacc agtgggtaga aaaatatttt ccagatacca cgattattga taaagaagca
    11701 aaattaagcg ctttaggcaa tcggctgaaa gacatccaag aagctatcca attcatacaa
    11761 caccacggcg atagccgtca aaagaaactc catttgaaac gtcaagaact ttccttacaa
    11821 gacttggaag caaattattc cgaagaaaat ttcatgaata aattagaaga agtccttgat
    11881 ttaaaagaaa agtgggaaca gctaactgaa aaacgtgatc gtttagaaga agaggaagaa
    11941 tggcttcttc attggcaaca gctggatatt gctccaagta tgtatgcaag taagacgacc
    12001 atttttgaga tggggactgt gagtgcagca aattttgaaa gtttcaagga agaactaagt
    12061 aagcttgatg acgtttatat ggaagaaata gattatacgc caaaacaagt ttatctttcg
    12121 tatgttgctt taaaaaaatc agttgctgct atagaagaaa ttgctgggaa atacgggttc
    12181 aataaagaaa cgtatcctta tgaacaaaat cctaaagaaa aattgcaaga aaccaaacag
    12241 caattatcca ttgttcacga gcagcaaaaa aagataacga cagcgttagg tcagtgcagc
    12301 ggctatatcc aagaatttga atgggtagaa gaagtcacac ttgccgttgc agaacgggaa
    12361 aaagtaaaag aacggtttat ccatgccact tatctgatcg ttttgcaagg ttgggtagat
    12421 gcagaagaaa aacagaattt gatgcatgtg ttaaacgaaa ctgtatcaga agaagatata
    12481 tacgttagtt tcgaaacacc aaactcagaa gagattcagc aggaagtccc tactaaattg
    12541 aaaaaccatc cgcttatcga accttttgaa ttgctgactg aaatgtacag tctgccaaaa
    12601 tatgaagaag tagatccaac gccttggatg acaccatttt atcttgtctt cttcgggatg
    12661 atggtcgcag atgttggtta cggattgctg atgctcattg ggacaatact agctcaaaaa
    12721 ctatttgttt taccccgcgg gatgaaacgt ttcgtaaaat tctttgagat tctctcggta
    12781 ccatcgatca tctggggatt gatctacagt tcatttttcg gacaggcatt accaaaagaa
    12841 ctatttggta tccgcttgcc tttcccaatc ctatctacga cggatgacgt caatacgatt
    12901 ttgattttat ccgtgatttt cggtctgata cagatcttgg ttggattatt cgtggcagca
    12961 aaggaaaata tcaggaggaa agattacttg agtgctgtca gtgatggttt tgcctggcaa
    13021 gggatcctga ttgggatcgt tattgcattg attggtgtca tgttgataaa aaatacagca
    13081 tttgtttatc ttggcgcggg gctatcgatc attgctgctt tatgtatctt aatcgtgccg
    13141 attatccaat cgccgtctaa agtcaagggt gctgccaaag gagcgtataa tctttacgga
    13201 ttgactggtt atatcggtga tttagtcagt tatacacgtt tgatggcact aggtatttca
    13261 ggcggaagta ttggcgcggc attcaatatg ctagttgctt tcatgccgcc agccgctcga
    13321 ttcagtgtag ggatcctttt gatcatcgct ttacacgcac tgaatatgtt tttgacactg
    13381 ttaagtgcct atgtccatgg tgcacgattg cagtatgtcg aattttttgg aaaattttat
    13441 acaggaggcg gacgtgcctt tgacccgctg aaaacggcgg aaaaatacgt caacatcaat
    13501 cataagaaaa aaaacaataa ataattggag gatttttata caatgatgga ttacttaatt
    13561 agtcaaaatg gtggaatggt atttgcagta ttagcgatgg caacagcgac gattttttca
    13621 gggattggtt cagctaaagg tgttggaatg acaggtgagg cagcagcagc gttaacaaca
    13681 agtcaacccg aaaaattcgg acaggctttg attctgcaat tgcttccagg tacgcaaggg
    13741 ttgtatggat tcgttatcgc ctttttgatt tttatcaact taggaaatga tatgtcagtt
    13801 gtacaaggat tgaactttct aggcgcttct ctgccgattg cttttacagg tttgttttca
    13861 ggtatcgccc aagggaaagt tgcagcagca ggtatccaaa ttttagcgaa aaaacctgaa
    13921 catgctacaa aaggaatcat ttttgctgcg atggttgaaa catatgcgat tttaggtttc
    13981 gttatttcct tcctattagt attgaatgcc taagcataaa ggaggaaaag cagatgaacg
    14041 ccattgaaaa aatcatctct cagatgaatg aagcagcaga gcaagaaaga gcggcgttag
    14101 agcaagaaga gcgaatgaag atcgatcaaa actttgaaca aaaacgtacg caaatcgaaa
    14161 cagaacatca aaaacaaaaa gaaaaacaaa tcgaattact tgaaaaaaaa taccgtcagc
    14221 ttaggaatcg ccaacaagta gaagtacgac aagaaaattt gaatgcgaag caggaatttt
    14281 tacgacgatt gtttgcagat gcaatagctg aaatggagaa ttgggatgaa tctgaacaaa
    14341 tccagtttat caaaaattca ttgtattcat tacctttgac aggcaaagta acattcattg
    14401 ctggagaaaa atcagcagct tatctttctc agacattgct ggatgaatgg aacaatgaac
    14461 ttccctttat gatggtttta agtgatgaaa ctgttgctga tcaagcgggt tttctgataa
    14521 acgatcaagg agttcagtat aattttcttt tcagcagcct tgtacaagat atccaaggaa
    14581 cgatgagttt tgagattgcc aatcaattat tcgaataagg agggatcagt gtatggaata
    14641 ccacgaacta aatcccttga ttcgcggaag agagcttgaa cttttgtcca atgagacatt
    14701 tgaacggatg attcaaacaa attctgtcga agcacttggt gaaatattga aatccacgat
    14761 ttacagtcca tatatttatg acggatatga atatgacttc gaacagaatc ttttaaagga
    14821 gcagagccgt ctatttcgct ggttaaagga atcagcaccg gagcctgaaa tcgtttggat
    14881 ctatacgatg cgctatactt tccataatct gaaggttttg acaaaagccg agatgacaaa
    14941 tcaaaatctg gaccatttgt atatcgacga tggattttat tcattagaaa ccttgaagga
    15001 tgccattcat acgcaagttt caaatgaact ccctgaaagc attatggaca gtatacgaga
    15061 agtacgtgat tattttgaag agtctaccat tcttcaggga attgacgtga tttatgatcg
    15121 acagtttttg acagagcagc gacggcttgc cgataagcta ggatatcaag aattgctgac
    15181 agaagtcatt gcttttattg acttgaccaa tatcacaacg atggctcgag gaatattgca
    15241 gaaaagaaca tatgggttca tgacgacggt actttccagc tcgggaagta tcgaaaaaga
    15301 agtatttttg aactttgtag ataaagatat ggaagattac acgaattttc tattagagac
    15361 agattatgct gattttctgc gccctgtcat ccgtgaggga aatcttgatc tcgtaaagct
    15421 tgaacagttg aaagatgatt atctaagttc attgtatcaa gaagcacaaa cccaggcttt
    15481 cggaccactg ccactactag cctttttgaa cgcaaaagag gtagaaagca aaaaccttcg
    15541 tttactggtt atcggcaaaa gaagccattt cgaaccagaa acaatcagaa agcgggtgag
    15601 acaagtctat gacgcataag atcggtgtag ttggcgacaa agattctgtt ttgccattca
    15661 aattgtttgg ctttgatgtt cgttatggta caaccaagca aaccgttcgt caggcaattg
    15721 aagaaatggc aaagaatgag tatggcgtga tttatgtcac agagcaatgt gcagcattga
    15781 gcccagaaac gatcgaacgt tacaaggaac aaatgatacc tgctatcgta ttgattccca
    15841 atcatcaagg atcattaggg atagggctgg aagaaattca aaaaaacgta gaaaaagcag
    15901 taggtcaaaa tatcttatag tatgaaaagg agatagaaat tttgcaaatt ggaaaaatca
    15961 taaaagtctc cggacctctt gtaatggctg agaacatgtc tgatgccagt atccaagaca
    16021 tgtgtctagt gggagatcta ggagttatcg gagaaattat cgagatgcgt ggcgatgtgg
    16081 cgtctattca agtatatgaa gagacttctg gaattggtcc aggcgaaccg gttcgctcta
    16141 ctggtgaagc tttatctgtt gaattaggac cagggatcat ttcacaaatg tttgacggaa
    16201 tccaacggcc attagatacc tttatggaaa tcacacaaag taatttctta gggcgcggag
    16261 ttcaattgcc cgcattggac catgagaaaa aatggtggtt cgaaccaaca gtagaagccg
    16321 gagaaacggt gagtgctgga gatgttatcg ggatcgtaga agaaacaaaa gttataaaac
    16381 ataaaatcat ggtgccaaat gggataaaag gaacgatcaa aacaatcgaa aaaggttcat
    16441 tcactattga tgaaacagta tgtgtcattg aaaccgaaaa tggtgacaaa aatttgacga
    16501 tgcttcaaaa atggccagta agacgtagtc ggccaatcaa agaaaaatta aacccggatg
    16561 cacccatgat cactggacag cgagtgattg atacattctt cccagtgaca aaaggaggag
    16621 cagcagctgt acctggtcct tttggtgcag ggaaaacagt cgttcagcat caaattgcaa
    16681 aatgggccga tgtggacatg gttgtctatg tgggatgtgg cgagcgtgga aatgagatga
    16741 cggatgtttt aaatgaattc cctgaattga ttgaccctaa tacaggagaa tcgttgatgg
    16801 aacgtacggt attgattgct aatacatcaa acatgccagt agcagctcga gaagcttcta
    16861 tctatacggg aatcacaatt gcggaatatt tccgtgacat gggttatgac gttgctatca
    16921 tggcagactc cacttcacga tgggcagaag cacttcgtga gatgagcgga cgtttggaag
    16981 aaatgcctgg tgatgaaggt tatccagctt atcttggttc acggcttgcg gaatactatg
    17041 aacgatctgg aagagtcatt gcattaggat cagaaggtcg cgaaggaagt ataaccgcaa
    17101 tcagtgctgt atccccgtct ggaggggata tttcggaacc agtcacacaa aatacattgc
    17161 gggtcgtaaa agtattctgg ggtcttgatt ccagtttagc ccaaaaaaga cattttcctt
    17221 cgatcgattg gatacaaagc tattctcttt attcaacaga agtagggaag tatatggatc
    17281 atatccttca agctgactgg gcatccatgg tcaccgaggg catgcgtatt ttgcaggaag
    17341 aacagcagct gaatgaaatc gtgcgtttgg tagggattga ttccctttca gataatgacc
    17401 gtctgacatt agaagtggca aaatccattc gtgaagatta tttgcagcaa aatgcttttg
    17461 atgatgtcga tacattcact tcaagagaga aacaattcaa aatgttgaag atgatcttga
    17521 cctttggtga agaaggacgc cgtgcgttgt cgttaggtgc ttatttaagt gagatcatgg
    17581 acggtaccgt atcgattcgt gaaaggatcg gacgaagcaa atatatcccg gaaacagaat
    17641 tagaaaaact agatcaaatt attggtgaaa tcaagcaaac gatgcaaacg atcgtttcag
    17701 aaggaggcat gagcaatgat taaagaatat cgtacgatcg gtgaagtcgt cggtccattg
    17761 atggcggttg aaaaagttgc tggtgtcaaa tatgaagaat taatcgaagt ccgtatgcaa
    17821 aatggcgaga ttcgtcgtgg acaagtattg gaagtccaag aagataaagc aatggtccaa
    17881 atattcgaag gaacaagtgg tatcaattta cgtgactctt ctgtacgatt tttaggccat
    17941 cctttagaac taggtgtatc ggaagacatg atcggtcgag tattcgatgg tttgggacga
    18001 ccgaaagata acggaccaga gatcctgcct gaaaagcatt tggatattaa cggagaagta
    18061 atcaatccag tagctcggga ttatccagat gagtttatcc aaacaggtat ctctgcaatc
    18121 gatcatttga atacattagt tcgtgggcaa aaattaccag tattttctgg ctctggtctt
    18181 cctcataaag aattagctgc tcagatcgcg cgacaagcaa cggtactcga ttcatcagat
    18241 gatttcgccg tagtctttgc tgctattgga atcacttttg aagaagctga atttttcatg
    18301 gaagattttc gtcaaacggg cgcaatcgac cgttctgtca tgtttatgaa tttagcaaac
    18361 gaccctgcaa tcgaacgtat cgctactcct cgtatggctt tgactgctgc agaatatctt
    18421 gcttatgaaa agggaatgca tgttttggtt atcatgacag acatgaccaa ctatgcagag
    18481 gcattgcgag agatttctgc agcacggcga gaagttccag gaagaagagg atatcctggg
    18541 tatctttaca cgaacttagc tactttattc gaacgagcag gaagaatccg cggactaaaa
    18601 ggatcggtta ctcaaattcc gattctaaca atgccagaag acgataaaac tcatccaatc
    18661 cctgacttga ctggttatat tacagaagga cagatcatct tgactagaga gctttacaag
    18721 agtgggattc agccaccaat tgatgtactc ccgtctcttt cgcggttaaa agataaggga
    18781 actggacccg gaaagacaag agaagaccat gcagctacga tgaatcagct ttttgcagcc
    18841 tatgcacaag gaaaacaggc aaaagaatta gccgtagttt taggtgaatc tgctttatct
    18901 gatgtagaca agatctatgc gaagtttgct gagcggtttg aaaaagaata tgtcaatcaa
    18961 ggattctaca cgaaccgttc gattactgaa acacttgatc tagggtggga actgctatcg
    19021 atgttgccaa gaaccgaatt aaaacgaatc aaagacgaca tgctggacaa atatctgcca
    19081 gaagggaagt gagttctgat ggcacgattg aatgtgaatc cgacccgcat ggaattgact
    19141 cgattgaaaa aacagttgac aactgccaca agagggcata aattattaaa agataagcaa
    19201 gatgaactga tgcgtcaatt catcctgttg attcgaaaga acaaccagct gcgtcaagaa
    19261 atggaagagt ctatgacaga agcaatgtct gattttgtat tggcaaatgc ttcgatcacg
    19321 gaacctttca tcgaagaatt atttgttttg ccggcggaaa acgttgattt gtctatcgta
    19381 gacaaaaata tcatgagtgt aaaagtacct gtgatgaatt ttgaatatga tgaacagctg
    19441 acagacacac ctttggaata cgggtatttg aattccagtg ccgaactgga tcggtcaatc
    19501 gatcggttta ctaccctgct tccaaaactt ttggaactga cagaaatcga aaaaacttgt
    19561 caactgatgg cgagtgaaat cgaaaagaca cgtcggcgag tcaatgcatt agaatatatg
    19621 acgatccctc aattagaaga aacgatctat tatattcgta tgaagctgga agaaaacgag
    19681 cgcgcagaag taacaaggtt gatcaaagta aaaaatatgg gtaccactga ctaagtggta
    19741 tcttttttta aaagaggaaa acgatgatta aacaaagcaa acaaataaag aaacgtttgc
    19801 cggctgttca gcttctcgca ttaggatttt ttctcctgat tttagttggc ggcggcctac
    19861 ttaccctacc attttttagt aatagtgggc atgggacaaa ttttatcgat gcgcttttca
    19921 ctgcaacttc tgctgtctgt gtaacgggat tgacaacatt gaatacagca gagcattgga
    19981 acagtgctgg tcaatttttg atcatgcttt tgattgagat cggcggtctt ggttttatga
    20041 tggttccgat tctatgcttt gcattgataa agaagaaagt cagtctcagc acacgtatta
    20101 tattaaaaga agcgctgaat ctagaagaag tatcaggtgt gatgaagctc atgctttaca
    20161 tcctaaaatt ggcctttgtt atccaattga ttggtacggt tgctttatct tttgtatttg
    20221 tgcctgaatt tggctggttg aaaggtatct ggtacagttt gttccatgca atctctagtt
    20281 tttgtaatgc agggtttgat ttgttaggag acagtttagc caatcaccag caaaatgttt
    20341 atttgatcat ggtggtatct gctttgatca tttctggtgg gttaggcttc atcgtatggc
    20401 gtgatttact ggaatatcat cagaaaaaga agatgacgat tcattcaaaa atcgcattaa
    20461 ccgttacagg taccctttta ttaggcggat tcgttgtgtt ctttttcaca gagcgcaatg
    20521 gggcaacgct agtggaaggg aatatgtttc aacgattggc aaacaccttt tttatgagtg
    20581 tgacacctag aacggctgga tattattcag ttgattacct ccatatgagc catgcaggtc
    20641 tgattttaac gatgtttttg atgtacatcg gcggaacatc tgggtcgacg gccggcggat
    20701 tgaaaacaac tacgttaggt gtgttgctga tccagatgcg ttcgatgttg aaaggacgta
    20761 cgagagcgga agcatttggc cgtaccatcc gacaaggcgc agtgttgcgg gcacttactt
    20821 tattttttgt tacgttgacg ttgtgtgtag tcgccatcat gattttatcg gtgactgaaa
    20881 cgatacctaa aacttctggc atcgaatata ttgcttttga ggtcttctct gcgtttggta
    20941 cagtaggtct tacgatggga ttgacgccag acttgactgc aatagggaaa ttgatcatta
    21001 tagcgcttat gtacattgga cgagttggtg tattgactgt ggtcttctct ctgatgacaa
    21061 aagcacaaag aacacaatca aaatacaaat atccagaaga aagtgtcatg attggataat
    21121 ttcatcttct gacagaaacc tagtgattta gtaaaataag aaaataaaat aatttttctt
    21181 gcttcttgga tactttaagt gcaaaatgtt cgtttatttt ttgaggcact acccgttgtt
    21241 gaacctcggg tagtgcctct tttttattga aaggaatcaa taagatttga ttcctttatt
    21301 tatcaatagg ttataattca tattttttta ttttttcttt tacgatactt agtccagaag
    21361 ctaaaatttc aaggttatta gaggataggg aaattcgaac gaatgcatct ggttgcgcac
    21421 gtgtagtaaa gcgtctggaa tgataaagtc ggataccgtt agcaagaaag tcattctcaa
    21481 ttatcttagg gtcaatctca ggatcaactg gaagaattcg atagtaaggg acacgattcg
    21541 ttggtttttc tactttgaaa agagaatcaa aaagctgatt ggctcttttt gtaagactta
    21601 atttagtttc taagatccta tttgcttcat ttgaatggag gatttgagtg atgacttcca
    21661 catctaatcc agaagttttc acatttatat taaaaattgc ttgttctatc ttgctttgga
    21721 catgtactgg aactacaaga taagcaactc gcaatccaga acaaataaat tttgtcattc
    21781 cagctaagta gattgtttgg ttaggtaata aattttggaa aggaggaatg atcgtatgtt
    21841 gatagtaagt tgttaaaaat gaatggatat catcttcaat aacccataga ccttcttttt
    21901 caatcacttc ggctaattgc tctcgtcttt tgtgagagat ttgaaaccct ataggattat
    21961 tacaagaagg cataagaaag acaccatgaa tcttcttttt tctacattcc tgaatcaata
    22021 tatcaggtcg cattccttgc tcgtcaaaat caattggaac gatctctaaa tgatataact
    22081 gagccaattc aatgaaatta gcgtatgtat atcggtctgt tgctattcta tcacctggag
    22141 agaaaagtgc agaaagtgtg atagccaatc cattttgaac accggaaaca atagatagag
    22201 attctaagga cggaatgact ccttgagttt ttaaccaatt tgctgcagta gtcaattgaa
    22261 aatttgtgcc tttgggatct tggtaattaa gcaaaccaac aagatttggg ttattactag
    22321 ttgattgaat aaacgggaca acgaatttat tacattcttc aaaagaacta accagaccaa
    22381 aatcaatgat atgttcggtt acttcattgg tagagatggt gatggactca aagacattgg
    22441 gagaaataaa tgtgccactt ccaatattcg tatacagcaa acctttctca attccatatt
    22501 tataagcctt gccaacggta gtaaaattta aatctaaata atcagctagc tctctttggg
    22561 agggtaactg agtgtttttc tgtaatttat tctttaaaat atcttgttct aattgttgaa
    22621 tcaacgatcg ataataagga cgcttcaatt tttgtttgtc tggtcgccaa gttaacgggt
    22681 aattgtcata agaattaata ggcattttct ccactcctta aaattgtata ccatacaatt
    22741 ttaccattga ttgtattgat tttagaagtt aatcttttgt gaggagggaa ttgtctatga
    22801 aaattattaa ttttgaggaa gtagtagctt cttttagttt tgaagaagcg attcaagtaa
    22861 tgaaaaaatg tttttttgat tatgaaagtg gtaaaatcag ccaaaatcct cgattggtgg
    22921 aaattttacc tgacggaaaa ggcaaaaata tgtttgcgat gatgcctgct tatttggggg
    22981 agaacagata ttttggcaca aaagtcatta ccgcttttcc ggataatcat cagtatcatt
    23041 taccttctca tttaggagag attttcttgt ttgatgctca aaacggacta cccaaagcat
    23101 tgataaacgc aaatgcagtc acctggattc gaacagctgc tgtatctgcg ctggcaacag
    23161 attacttagc aaatccgcat gcagaaacac ttgctttaat tggagctggt caacaagcat
    23221 tgtcgcattt gactgcgatt tctaccattc gtcccatcac tacagtgttt gtttacgata
    23281 ttttagaaga aagacgaaat acttttatcg ctaaagctaa aaaacagtat cctaaaataa
    23341 cgtttataaa tgcaatatca gtagaagatg cagtacgtcc agcggaaatc atttgtacgt
    23401 tgacctctag taaagaggca tttcttcaaa aagagtgggt aagggaagac gcacatatca
    23461 atgctgtggg cacatttact cctgatacaa gagagataag tacatcgttg atggaaactt
    23521 ctcaaatcta tgtagatgat tactcagctg ccttaacaga aagcggggac ctgcttatag
    23581 ccatggcaga aggagattta gaggaatctt gtattgttgg tacattaggc gaattagttt
    23641 ctgagaaaaa aacattttat aaaggagaat cgaggccgac gattttcgac gcagttggtc
    23701 tagctattga agatctttgt tgtgcagaat atatttataa caaattagag aaaggggctt
    23761 taaaatgaac ttagattatt acgtagttga tgcatttgca gatgaggtgt tcaaaggaaa
    23821 tccagcagcg gtttatgtgc tagaagaatg gctgccagaa ggtacgatgc aaaaaattgc
    23881 cattgaaaac aatttatctg aaacagcatt tacagtgaag aaaaaccaag aatttgagct
    23941 tagatggttt acgccagatc gagaaatcga tctttgtggt cacgcgacat tagcaacagc
    24001 atttgtttta tttaactatt acaaaatacc tgatgaaacg attaaatttt ctactcaaag
    24061 cggtaatcta tttgtgacaa gaattaaaga ttattattac atggattttc ctagtattat
    24121 gccgaaaaaa gtgccgatat tgtctgaata tgaagaagcg attggggcta agattaaaga
    24181 agcctactta gcacgtgatt tatttttcgt attagaagat gaaaaaacgg tggctaaatt
    24241 gcagccagat tttacagcta tcaaaaattt tgaattagga ataggagtca tcgtgacagc
    24301 agaaagtgtg caaaaagact ttgtttctag aaccttcttt ccaaaattaa caatcaatga
    24361 agatccagta tgtggatcag cacattctaa tcttattcct tattgggcag aaagaatgaa
    24421 taaaacaaaa ttgtcagctt atcaaatgtc atcaaggggc ggggatctag aatgtgaatt
    24481 aaaagaagat cgtgtgataa taggaggaaa agcagttttg ttttctaaag gtacctcgtt
    24541 tatttgattt ctttcaaatg aattaaaaat aaattggtgg agagtggcaa ccttatagct
    24601 acaatggttg ttaaaaaaag acgcaaactt tttcaattta atgaaaagtt tgcgtctcat
    24661 tttaattatt ggtgtttgtt ttttattcaa tcaagagtaa aatgccccat actaactatc
    24721 ctcggatgac ttcgatttta taaccatcag ggtcaatgac aaagtagtaa gaaggcgcag
    24781 tgccaggtaa tccttttaag tcagtaatct caagaccggc agctttatgt tgctcgtgta
    24841 atttttctag atcatcagct gctatagcga tatgaccata gccgtctcct agatcatacg
    24901 caccatgatc atagttgtaa gtcaactcta attcatagtc atccccagga agtgtcaaat
    24961 aaacaagtgt aaatttgttt tctggaaaat cacggcggcg gctttcttca aaaccaaatg
    25021 ccttttgata aaattcgatt gatgcttcaa gatctttcac tcgaacacat gtgtgagcca
    25081 ttttcataga ttcatctctc cttttccttt tactcaatca taacaaaaag aagtaaggaa
    25141 atagttgatt tgtgcttgtt catgagaaat ttctcatgta aaatggatag aaaataaaac
    25201 tgttaaggtg gaaactattc atggataaac caatttttgg tgaaaaaaag caatttgctg
    25261 attatcaaac ccgttatgca gcatatattg tcatagaaag agatcagcag attgcagtaa
    25321 tcgaggctcc aaatggtgct ttttttcttc ctggcgggga aattgaagga gaagagacga
    25381 aagaagaagc gattgtccgt gagctgatag aagaaatggg aattgtggca gaaatttctt
    25441 cttatctggg agaagctgat gagtattttt actcgaatta tcgtgcaact tattattata
    25501 atccaggata tttctttgtt gctgattctt ggaagcgtat aggagaacca actgaaaaga
    25561 ccaataaaat ttggtgggtg agccctcaag aagcgcttgt gaaattgaaa cgtggtagcc
    25621 accgatgggc agtcgagaga tggatcgaaa aaaataaaaa ctgaaagaaa gcgagtagat
    25681 cagctttctc tcagtttttt tatttattac agtacatatt ttttgattgc ttcagctaca
    25741 ccgtgctcgt cgtttgatga agtgatgaca tttgctgctt ctttgacaag agaaacggca
    25801 tttcccattg ctactcctag accagcatat tcaatcatgg ataagtcgtt ctcgttatct
    25861 ccgatagcca tgatttcatc ttgatcaata ccaaggtgct gtgcgagatt cgcaactgcc
    25921 gcacctttac ttgcttcttt atttagtact tcgaagtaat agggcgcact tttgaccgtt
    25981 gtgtatttat cacggaaagt ttgcggtaaa cgcgcaattg ctgcatctaa aatttctggt
    26041 tcgtcgatca tcatcatttt gacgatgttc atgtctggtg tcatttcttc aggtgttcga
    26101 tattttagcg gcatcttgac taaacttgct tcgtggatcg tataagcgct gatatctcga
    26161 ttaggcgtgt agatcgcttt gtcatcgatc gaatggagat gactgccgac acggcgtgcc
    26221 atcacttcga tctctaggaa atcttcatgt gtcagtccgt gacgggcgat gattttacct
    26281 gatttcgttt gttgtacgag tgccccgtta taagtaataa cgtaatcatt atcttgaaaa
    26341 aggttcaact cgtttaactg ttcctggaca cctggtaagg gacggccagt gcataaaacg
    26401 atcgaaacac cttttgcaac agcagcggca atcgcttctt ttacttcttt tgttacttct
    26461 cttttctcat ttaataaagt accatcgatg tcgatagcga ctagtttgat agacataatt
    26521 tcctccaata tattttaatt tatttcaatc aatgcgccat tacggatatg gctggcaaat
    26581 tcttgataag tttcgtcaaa taaatcataa tgatcacgca aggacggatc aaccatttct
    26641 tttggaaaga aaaatcgctc atctccttgt tcttgtcccg ctaatgcagc aacaagctga
    26701 ctaacctgtg ataattctat caaagttccg tcatttcgca aaatctcgat ttgtgtgcga
    26761 tgtctgcctt gttctgggcg ataaaaatca taaggtagat cgtagctaga attcacagca
    26821 gtgtaataaa caggattata tccgaccttt tctactaaca aagtgagttc ttggactaat
    26881 tttgaatccc ggttgccaga aaaagtagca gatttcagtg gtttacgatt caaaaaacgt
    26941 ttagctaaat catttaaaat aggatcagca gattcagacc attgagtaaa gtaagtgccc
    27001 aagaccccat catcaagttt gaggtattct tctaaagtaa agcttccgtc aagaaatggc
    27061 agaagtaatt gagaatggag ttcaaaactg ctggcatctt cttgatacaa ctcgtgtgcg
    27121 cgatgaagca gatgctccaa aattacttcc ataccacggg aaacaggatg gaagtacact
    27181 tgcacataca tttgatatcg gctaacgata taatcttcca ctgcatgcat gccactcatg
    27241 gaaaaagcga tcccgccttc ataaggacga atcacacgta atattcgggt aagatcaaat
    27301 gttccatatt ctgttccagt gaaataagca tcccgaagca aatagtccat tcgatctgca
    27361 tcgatctgac tagaaatcat ttggacaact tgtgggttgg ggtaagtttt ttggatcaca
    27421 ctagctacct tttcaggaaa accttcttct actcggttaa gaatctgata cacttctgtt
    27481 tctggagaag tgatgatctc tacagtgatg gcttcatgat tcgtatggaa gatatgttca
    27541 aatgtatgag agtaaggacc atgaccgaca tcatgcaaca gtgccgcaca taaagtcacc
    27601 agccgttcct gatcatccca gcctccgttg cctatttttt cttttgaaaa atttcgagaa
    27661 aagatatcgc agattcgtct tgaaatctca taaacgccta atgaatgagt aaaacggcta
    27721 tgttctgctc cgtgaaaagt gaaagaagaa gtgcctaatt gtttaatgcg tcgtagacgt
    27781 tggacttctt tcgaattgat caaatctaag attacttgat gctggacatg gatatagtta
    27841 tggactggat cacgaaaaac tttttccata ggcagcattt gatatttata aggggttgtc
    27901 ataatgtagc tcctttatgt tattacgttg ttccattctc tggatttggt tgttccattg
    27961 ctccagctgg atttcttttt ttagccagtc ggtagaatcc tgtaaatcac ttgcagaata
    28021 cagcttttca aaagcttttg caaatgcttc tttgacttct tttacaaaaa ggggttggtc
    28081 aagcagttct tcaagtgttg ccattgtagc aggatccact gcaggatagc catttgtacc
    28141 aaagtcttct tttaaagcag attgataaaa tcgcttcatg acttgcccgc gtttttcctg
    28201 attaccgttg acacttaagt acatcataac agaaatccca tttttgatcc gacgctgagc
    28261 gataccggca aattttttgc catggatgct taaatcataa gttccaggac aataagaatg
    28321 ggtgatttcg aatgcttgta tcggtgcctt tggaaaagcg tatttagtca ataggagcat
    28381 ttgctcgtaa gcgtcatcta tggttatttt ttttgtgtca ctttggggga aaatcaaaga
    28441 aatattcaaa attccttcat ctgatacgat ccctaatcca cctgaatttc tgatcactgg
    28501 agcgtaacct gcatctttca gtgtttggat cccgtcactt agaaaaggag ttcgaacatc
    28561 cttcatacct aagatgactg tgcagtcata ttgccagaaa tgtaaaaaga tttgctggtg
    28621 ttctttagaa tattccgtta atatatctgt taatgcaaaa ggggaaaagt cgtttttttg
    28681 ataaatgtgc tgatctagtc gaatcgcagg gttcattcaa ccggctcctt tgtataaatt
    28741 catattttct acaatattat actctaaaat caaagagttg ttttggaaaa aaggttttct
    28801 gtaaaaatgg acaagttggt tgacaaaaca aaagaaacta ggaacaatgt agctatgaga
    28861 agtaaaggag aatgaataat ggaattatca caaggaacac cgatttcaat caaattgcgt
    28921 acaaaagtaa aacagcaagg ggaagtgcaa gatttttatt ttgatttgaa agggcaaatg
    28981 gtgacaattg gcgatacgct gtacatacgt tataaagaga ttcaggagga gacgggcgat
    29041 gagattcctg taacgatcaa attagttcca gatggtcatg tacagctgat ccgtgcagga
    29101 gaaatgcgga tgcgtttgaa atttggctat aaagaacggt tagaaacatc ctatcgaaca
    29161 ccatacggta tgatccagat tgctactttt acgaaagaac tgcatgttag tttgaaagac
    29221 cgtcccacag gagggaaggt tcgaatcgat tatgatctgt tcatgggacc ggaaaaaatc
    29281 ggcgaatatt atttgacctt ggattttaca gcgtaaatat aaaagaaaag tttgattttt
    29341 tgtgtctgat tttgtatgat aaggagtaga ctgtgaaagg acgtgtcctg attggaaatt
    29401 agtgtatttg aaggtgcaaa caaaagcgaa ttatcaatga tcgaagtagc acacgcgatc
    29461 ttagaacaac gtggcgatgt catggatttt tctgatttag ctaaccagat ccaaaactat
    29521 cttgggaaat cagacagtga aattcgtgat tcactcgcac aattttatac tgacttgaat
    29581 attgacggca gctttatctc attaggggat aatcgttggg gcttgcgttc atggtatgct
    29641 atcgattcga tcgatgaaga agtaaaccat ggaatggacg aagaggatga agatactcca
    29701 cgtcgccgta aacgcaagaa agtcaacgcc tttatcaatg atgatgaaga cgcgatcgat
    29761 tacaatgacg atgacccaga agatacagat ctgacagaag aagatgacga tgatctgttt
    29821 gatgacgatg atgatgaaga tgaagaaatc gctgcttata attctgactt gcaagaaatc
    29881 ggagcagatg acccgacaga tgatgaagaa gacttgccag gaaatatcga agaagattta
    29941 agtatcatag acgaagacga tgatgatgaa gattatgaag aggaagaatt ttctgactct
    30001 gaaaaagaat aaacattatt ttcttacgag aggttgtgac agagacgatc agcctatcag
    30061 aataaggagg tgtgacacga tttttgtcac accttctttt ttgtatataa aaatcccaag
    30121 taaatacata aaaaataaaa aaattgaaac ttttagcgca ccttgtacgt catattaagt
    30181 gaggcagaaa ggagtggaga atgattttta ctggatataa gaaaaaacgg cagatcgaaa
    30241 aataccgtca cctgctgaat ggactcatcg aaactgatta tcaaaaaatg ttccgtatca
    30301 cgatgaatta cgtacatgat aaagaagagg ccttagatgt gatgcaagat agttttcata
    30361 aagccctgaa ctattttgaa aaagaaaaag agatcgagca ttttagtgca tggttttatc
    30421 ggatattgat ccgcacggca ttagatagtt ggcgcagaca aaaaaggacg cctgtggact
    30481 tatttgaaat tcaagatatc ccccaacagt tacctttaga aacttccgtt gcagagctcc
    30541 atatgatttt agataaaata gacagtccgg aaaaagagat tctgatcctc tacttttttg
    30601 aaggatttcg tttgaaagaa attgcgacta tcttggatat gaatgaaaac acagttaaaa
    30661 cgaaaatgta ccgctcattg aatgctttac gtcaaatcct agaatgaagg agagaagagt
    30721 gccatgtatt cccaaaaaga aaatttaaaa aaactaaaga aaacttataa agaacaagca
    30781 attcccaaag ggcttaaaga ggagatccgc cagcgtttta ttatcgaaga gcaatcattt
    30841 ataaaagcaa agcggagaaa gagacgtctc caagggttcg gagtatcttt ggctatcgta
    30901 atgattacaa caagtagcct tctttttaat agtcaagttc gaagctttgc agaagattta
    30961 cccatcttag gatcagtcat tgaattgatc ttaggcgaac gctttactga tcgatctgaa
    31021 aaaatcgata ttcaggtacc gaagatcaat acacaaaatg agaaagaaaa taaaacaatt
    31081 catggattaa atcaaaaata ctttcgcgaa ggacaagcag attttgaaaa agctgaaaaa
    31141 gaatatggaa attttgaaac ggatcattac caagttttgg gagattatca gaaaatcgta
    31201 gacgataacc ggtttctcgt gattgaaagg cagatcaccc aaacagcagc agatagccat
    31261 gttgaaaaaa gatacgacac aatcgacaag aaaaacagtg tccagctctc acttccactt
    31321 ttattcaaag atgacaccta tctatctgtt ttgacaaaag aagtcaaacg gcagatggcc
    31381 gaacaagtca aggaagatcc atctaagtac tattggactg agcaagatat tcaagaaggg
    31441 acgatagaaa aaccaacact tgttacgcct acacgaagtt tttatttgaa caaagagcat
    31501 caattagttt tgactttttc tcaatacgaa attgctcctg gttatatggg tacaccagaa
    31561 tttgtcattc ctaagtccgt gacaaaaacg attttggcat cggaagatta tttggatcat
    31621 tagacgagaa gtagttgcga ctagcagttt caattttcaa gtaagtacaa gtaaataata
    31681 agaagagaaa aagagaaaga agagatatag tgaaaagaaa ttatttagtt atcagtatta
    31741 ttgctttatc cgtgcttttg tttttcgaaa ttacttcggt ggcctccttg atcaccgaag
    31801 taaaaggaca aacacactct tcggagcatt cgtcacaatc tttcaaacaa tcagaacaat
    31861 caaccattaa aagccaatca actgaaacag ctaacgtcaa agaagccatt caagaatcag
    31921 cggaaaaggt acaaagtcag tcatcacaat caacacaaga cagtacaaaa acgagccgag
    31981 atccggctgt caaaagcgtc gcaatcagct ttgatgatgg accaggagcg accacaacac
    32041 cgcagcttct gcgaatctta aaagaaaaaa acgtgcatgc cacttttttc gtcttaggag
    32101 aaaatacggc gcagcatcct gaaatcgtga agcagacagc tgaagcaggg cacgagattg
    32161 gcaattacac atacgatcat cagaatctgg ccactctttc tgcccagtcg atgacggagg
    32221 aagtgacgaa agcagacact gagatcaaaa aagtcactgg caaaaccccc acttttgttc
    32281 gtccgcccta tggttctgtc acgaatgtag gagcaacgat catccagcga ccgatcatcg
    32341 aatggtctgt tgattcagaa gattggaaga caagaaatcc tgatttgatt ttgcagaaga
    32401 tccaagcaac agtatacgat ggggcaatta ttttattcca tgatatctat cctgaaacga
    32461 ttcgtgccgt tcctcaagtg atcgattatc tgaaagagca agggtatcgc atcacgacag
    32521 ttggggattt attagggcat ccaacggctg tcgagaacta ttatggcaga aacgatcatc
    32581 ggccagttca gtaagtgaga caagtatctt taataaataa cacattttaa taagtagttg
    32641 aacacataac taccttactt gcaaaggctt aacaatatct atacaggtac aacaaaaaac
    32701 tgtccaccaa ttttctttgg cggacagttt tcacttaaaa ataaagaagc aactattcgt
    32761 aaattatgct aaaatagcag tagaacgcgt cctgagggaa tcgaaccccc gtctcaagaa
    32821 ccggaatctt acgtgatatc cactacacta aggacgcaag cactgggata attgtaacta
    32881 aacagataaa aaattacaag gggattggac aaaaaaatga aatttgagca aaatttttca
    32941 caaaaacagc agcagacaca gaaactagcc atgacgcaac agttgcaaca gtccatccaa
    33001 gttttacaat ataacagtga ggagctgttg gcattcgttg agaatcaagc aatggaaaat
    33061 ccattggtgg aagttgttga gccggaatgg cagccagatt acataaaagc ttcttcatct
    33121 tcttatgaag gagaagagac aaattaccta aatcagattc ctgatacaaa agggtcttta
    33181 tttgattcat tgatcgaaca agtccatttg aattaccgag atactttttt gaggaaaatc
    33241 gttctttatc ttgtagaata catcgatttg aacggatttt tgacgattag tttagaagaa
    33301 gccggaaaag agattggagc tacgcctatc caaatgctcg atgcgttgac tctgattcag
    33361 caattagaac cagctggagt aggtgcacgt agtttgcaag aatgtctcat gcttcaaaca
    33421 gaacgagatg attatgcgcc ggaattagct tatatcgtgt tggaagaatg ttttgaggaa
    33481 ctggtggagc gaaaatggaa agaaatcgca caacgttttt ctgttgatct acatgctgtt
    33541 cagcagatat tcgactattt acaaacgttg agtccaagcc ctggccggat tttcgaccgt
    33601 agcagcgaat tgtacattat tcctgatgtc cgggtattag tggacgacga taaaaatgta
    33661 caagttatct caaaccgaaa aaatcagcca aatatacgct ttcaagagag ttacttcaaa
    33721 caaatgtcac aacaagcaga taaagaaact gaaaattatt taaaagaacg gaaacaggaa
    33781 ttcgaatggt tgaagaaaac gatcctacaa cgtggggata caattttgcg ggttgctcaa
    33841 gtcattgttt cccggcaaaa agctttcttt atcgacaagg aacgtccaat caagcctctt
    33901 acgttaaagg aagttgcaac tgagatagat gtgcatgaat ccacggtcag tcgtgcagtc
    33961 aacgggaaat atttggaaac agatttcggt gtatttgaat taaaaaaatt tttcactaca
    34021 cgaattccta cgaatagtac agaacagaca gaagaccttt cagcagatac agcgaagaaa
    34081 aagctgcagg aattagtcga tcaagaagac aaaaacaagc cgttgtcgga ccagaaattg
    34141 gttgaattgc tgaaaaaaga tgagatagcg atctcaagac gaacagtagc aaaatatcgt
    34201 gatttattag gaatcccgag ttcatcaaaa agaaaacgtt atgataacta atcagtcaaa
    34261 agactagaaa aagtatttca ctttttctag tctttttagt tgaaaaacga gagcgaaact
    34321 gttagactta cagtcgtgag gttgagattt ccctttattg atgtgaaatt cgaatagtta
    34381 atttcttttt ttatcgtata tgggtcgttt taagtctaca ttggacgtaa agtgtccaac
    34441 taaggagatc gctatgctgg atgaaataaa aatgattgaa tctgttgcac cagatattat
    34501 cgaagtgttg caagaacgct ataaaatttt acgaaatatc tattggatgc agccgattgg
    34561 gcgtcgaagt ttatcggaaa gtatggggat cacagaacga gtcttacgaa cagaaacaga
    34621 ccttttgaaa aatctgaatt tgatcgacac ctcaaaaagc ggtatgacgc tgacaactaa
    34681 gggcgaagaa gtttatcaaa gccttgaaaa cttcatggat cagcttttag gtacgcatca
    34741 aaccgaacaa cagttggcag aatattttgg taaccaacgc tgtatcgttg tttcaggaaa
    34801 cagcgaagag cagaccaaag tggctgatgc attcggcgaa gcgctgagcg aagccttaga
    34861 ccgactactt ccagaaggcg agaatatcat cgctgtgatg ggaggaacta ctatggctgt
    34921 ggtagccgaa caattgtcta atctggaaaa taagaaacgg cataacctgt ttgtccctgc
    34981 aagaggcggg attggtgaag cgataactgt acaggcaaat tctgtcagtg caagaatggc
    35041 tgctaaagca aacggtaacc accgcgcact ttatgttccg gaacaattaa gtctggcaac
    35101 ttacaattct ttactcaatg agccatctat ccaagaagtc ttaaacttga ttagtgaagc
    35161 gaattgtgtt attcatagta ttgggcgtgc attacacatg gcagcacgtc gtaagatgac
    35221 tgaaaaagaa ttagtcatgc tgaaacaagc aaatgctgtt gcggaatctt ttggctactt
    35281 tttcaatgaa aaaggtgaag tcgtttataa ggtccctaga atcggcatag aattgcaaga
    35341 tttggaaaaa gtcccgatca tcatggcaat cgcgggcgga aaatcaaaag ctaaagcaat
    35401 tcgagcttat atgaaaaatg caccaaaaca aacgtggctc atcactgatg aagctgctgc
    35461 aaatgagatt ttaaaagggg taacccttta aaataaatat ttttttgatt ttcataagga
    35521 ggaaatctta aatgacagtt aaagtaggta ttaatggttt tggacgtatt ggacgtctag
    35581 cattccgtcg tatccaagat gtagatggaa tcgaagtagt agcaatcaac gacttgacag
    35641 atgctaaaat gttggcacac ttgttaaaat acgacacaac tcaaggacgt ttcaacggaa
    35701 cagttgaagt tcatgaaggt tcattcaacg taaacggaaa agaagttaaa gtattagcta
    35761 accgcaaccc agaagaatta ccatggggcg aactaggcgt agatatcgtt ctagaatgta
    35821 ctggtttctt cacttctaaa tcagcagctg aaaaacattt aacagctggt gctaaacgcg
    35881 ttgttatctc tgctcctggc ggaaacgatg ttccaacaat cgtttataac acaaaccacg
    35941 aaacattaac tggtaaagaa acagttatct caggtgcttc atgtactact aactgtttag
    36001 ctcctatggc taaaacattg aacgacaaat ttggtgttgt tgaaggatta atgacaacta
    36061 tccacgctta cacaggtgac caaatgactc tagacggacc tcatcctaaa ggtgacttcc
    36121 gccgtgcacg cgctgctgct gcaaacatcg ttcctaactc aacaggtgct gctaaagcta
    36181 tcggtttagt aattcccgaa ttgaacggta aattagacgg agctgctcaa cgtgttcctg
    36241 taccaacagg ttcattaaca gaattagtaa cagttcttga aaaagaagta actgttgacg
    36301 aaatcaatgc agcaatgaaa gaagcttcaa acgaatctta cggatacaac acagacgaaa
    36361 tcgtttcttc agatatcgtt ggtatgactt acggttcatt atttgatgct acacaaacta
    36421 aagtaatgac agttggcgac aaacaattag ttaaaactgt tgcttggtac gacaacgaaa
    36481 tgtcatacac tgcacaatta gtacgtactt tagaatactt cgctaactta taagatagac
    36541 aaacgtacaa gtgtaaatta tagacatgta catttaaaag cggggaagca atcgcgcttc
    36601 ttccgctttt tttgataata agtttattca ggaggaaata aaatggctaa gaaaacagtg
    36661 aaagatatcg atttaaaaga caaaaaagtt cttgtccgtg ttgacttcaa cgttcctttg
    36721 aaagacggcg tgatcactga cgacactcgt atcaaagcag cattgccaac aatcaactac
    36781 gttctagaac aaggcggaaa agcaatcctt ttctctcacc ttggacgtgt aaaaacagaa
    36841 gaagataaag aaggaaaatc tttagctcca gttgcaaaac gtctaggcga attattaggt
    36901 aaagaagtaa cattcgtacc tgaaactcgc ggagaacaat tagaagaagc aatccgcaac
    36961 atgaatgacg gcgacgttgt cgtattcgaa aatacacgat ttgaagatat tgacggtaaa
    37021 aaagaaagca aaaatgatac agaactaggt aaatactggg cttcattagg cgatgtattt
    37081 gttaacgatg ctttcggtac agctcaccgt gcacatgctt ctaacgtagg tatcgcttca
    37141 actggtatcc caacagttgc tggattcttg atggaaaaag aaatcaagtt catcggtgaa
    37201 gcagtagaag aaccaaaacg accaatgatt gcaatccttg gtggcgcaaa agtttctgat
    37261 aaaatcggcg taatcgaaaa cttgattcct aaagcagata aaatcttgat cggtggcgga
    37321 atgacttaca cattctacga agcaaaagga atcaagatcg gtaactctct agtagaagct
    37381 gataaagtgg aactagcaaa agaattaatc gaaaaagcag gcgacaaact tgtcttgcca
    37441 atcgacaatg tctgtgcacc agaattttca aatgatgttg aaacacaagt aatcgaagga
    37501 gacattcctg atggtttgat ggctttagac atcggcccag catcagtaaa attatttgct
    37561 gatacattac aaggtgcaaa aacagttgtt tggaacggac ctatgggtgt atttgaaatg
    37621 agtaactttg ctaaaggtac aatcggtgtt tgtgaagcaa tcgctaactt agaaggcgca
    37681 acaacaatca tcggtggcgg agactcagcc gcagcagcag aacaattagg atttgctgac
    37741 aaattcacac acatctcaac aggtggcggc gcaagcttgg aattgcttga aggtaaagaa
    37801 ttaccaggac tagcagcaat caacgataaa taaacgacca agtttgctca attctgacga
    37861 gtaatcgaac aaaaggtgaa gggagttttc atgactccct agccattttt caataaatta
    37921 aggagtgtgt tcctcaatgc gtaaacctat tatcgcaggt aactggaaaa tgaacaaaac
    37981 tgcatcagaa gcaaaagaat ttgctgaagc agtaaaaaac aaaatccctt caaatgacaa
    38041 agttgattca gtcatcggat ctcctgcatt attcttacaa gaattaatga catcagctga
    38101 aggtacagaa ctaaaaatct cagcacaaaa ctgctactgg gaaaactcag gtgcgttcac
    38161 tggtgaaact tcaccagcag ctcttgctga tctaggtgtt cattatgtga tcatcggaca
    38221 ttcagaacgt cgtgaatatt tccatgaaac agacgaagac atcaataaaa aagcgaaagc
    38281 tatctttgca aatggtatga caccaatcct atgctgcggc gaatctttag aaacctatga
    38341 agcaggcaaa actgctgaat ggatcgaagg acaaattaca gctggtttag ctgatctttc
    38401 agctgaacag gtgtcaaaca tggttattgc ttatgaacca atctgggcaa tcggtactgg
    38461 taaatcagct gacgcacaaa tcgctgacga aatttgtgga gttgtacgtc aaacagttga
    38521 aaaactttat ggaaaagaag tatcagaagc agttcgtatc caatacggcg gttctgtaaa
    38581 accagaaaat attgctgaat acatggcaaa agaaaatgtt gacggtgctt tagtcggtgg
    38641 cgcaagcttg caagcagatt cattcttgtc attgttagac gctgtaaaat aattgttgaa
    38701 aatttgcgaa aaatgcaaga aatgtttgca ctaatgagca aaattaacta aaatcgtata
    38761 tgagggaaat cccttaggaa aaaacaaact caaaggagag acaaaacatg tcaattatta
    38821 ctgatgttta cgctcgcgag atcttagact cacgtggtaa cccaacaatc gaagtagaag
    38881 tatacacaga aagcggagct tttggccgcg gaatggttcc ttcaggtgct tcaactggtg
    38941 aatacgaagc agttgaatta cgtgacggag acaaagctcg ttatggcggt aaaggtgtaa
    39001 ctaaagctgt cgacaacgta aacaacatca ttgctgaagc aatcattggc tacgatgttc
    39061 gtgaccaaat ggctatcgac aaagctatga tcgctttaga cggaactcct aacaaaggta
    39121 aattaggtgc gaacgctatt ttaggtgttt ctatcgctgt tgcacgtgca gctgctgact
    39181 acctagaagt acctttatac cactacctag gcggattcaa cacaaaagta ttgccaacac
    39241 caatgatgaa catcatcaac ggcggatcac atgctgataa ctcaatcgac ttccaagaat
    39301 tcatgatcat gcctgttggc gctccaacat tcaaagaagc tctacgtatg ggtgctgaag
    39361 tattccacgc attagcttca atcttgaaag ctcgcggatt agcaacttct gttggtgacg
    39421 aaggtggatt tgctccaaac cttggttcaa acgaagaagg ttttgaagta atcatcgaag
    39481 caatcgaaaa agctggctat gtacctggta aagacgttgt tcttgctatg gatgctgctt
    39541 cttcagaatt ctacgacaaa gaaaaaggtg tttacgtact agctgactca ggcgaaggcg
    39601 aaaaaacaac tgacgaaatg atcaaattct acgaagaatt agtttctaaa tatccaatca
    39661 tctcaatcga agatggatta gacgaaaacg actgggacgg attcaagaaa ttaactgacg
    39721 tattaggcga caaagttcaa ttagttggtg acgacttgtt cgtaacaaac actcaaaaat
    39781 tatctgaagg tatcgaaaaa ggaattgcta actcaatcct tatcaaagtg aaccaaatcg
    39841 gtacattaac agaaactttc gaagctatcg aaatggctaa agaagctggc tacacagcag
    39901 ttgtatctca ccgttctggt gaaacagaag attcaacaat ctctgatatc gctgttgcaa
    39961 caaacgctgg ccaaatcaaa actggttctc tatcacgtac tgaccgtatt gctaaataca
    40021 accaattact acgtatcgaa gaccaacttg gtgaagttgc agaatacaaa ggtttgaaat
    40081 ctttctacaa cttaaaaaac aaataattct tttctaatga agatacatga tcgactattg
    40141 aaagatagtc gagtatgatc acttagttaa gtaatcgaac tctctacttg ctgaaatatc
    40201 agcaggtaga gagttttttt gcaactagct tctgtatatg atgtttacaa agaatggtac
    40261 aagaaaagat catccttgaa aaaggaaagc taacaataac tagcatcata taagagcttt
    40321 taaggataga acagtagaat aggaatctat gggcaagtcc tctcgtataa ataaaatagg
    40381 agggaagtag cagcatattt ttcataaagt ggctctcttt cttcattttt actttggttg
    40441 ctcctatttg aaaattcgag cacactcaaa tacaataaaa agaaatggca agggagagga
    40501 ttagatgaaa aaagaagaaa aaatcgcgat tttacaagag attatccgaa ttaaatcagt
    40561 gaatggaaac gaaggtgaag tagcagccta tttgaataag cttttggcaa agcatgacat
    40621 aacaggagag attgtttctt acagagatgg tagagataat ttgattgctc gttaccaaaa
    40681 gggccaatca ggaaaagtgt taggcctatc tggacatatg gatgtcgtgg cggctggtga
    40741 tgaatcgtct tggacctatg caccctttgc agcagaaatc catgggaacc gtttgtacgg
    40801 acgtggtgct acagatatga agagtggctt ggcagcaatg gtgattgcca tgatcgaact
    40861 gaaagagtca ggaaaaccat ttaacggaac agtcaaatta ttagccactg tcggagaaga
    40921 agtgggtgaa ctaggtgggg aacagctcac gaaagcaggc tatgtagatg acttagatgc
    40981 cttgatcatc ggagagccaa ccaattacag tttgatgtat acgcacatgg gatcaatcaa
    41041 ctatacagtc acctcgcacg gaaaagaagc gcatagctct atgccagacc aaggttataa
    41101 tgcaatcaat cacctaaatg aattcattac gaaagcaaat gctgaaatga atcacctcgc
    41161 tgaaacaatc gagaatcctg tcctaggaaa aacaatccat aatgttacat tgatcagtgg
    41221 cgggaatcaa gtcaacagca tcccaagcca tgcgcagctc caagggaaca ttcgatcgat
    41281 tccagaatac ccaaatgata aaatcattgc attgctgcaa tcaatcgtca atgaattgaa
    41341 tcaagagaca gactatcatt tggaattgat gatcgattac aacaaaatac ctgtcaaagc
    41401 tgatccagat tctcctttga ttcattgcat tcagcaacaa ttcagccagc cattaccttt
    41461 agttggagcg gcagcaacaa cggatgcagc cgaatttacg aaagctaacc attccttcga
    41521 ctttgtcgtc tttgggcctg gtgtcgttac tttgcctcat caagtagatg agtatgttga
    41581 aatcgataat tatttggata tgatcgaaaa atatcaagga attattttat cctacttagc
    41641 gtaagatttt tttaa
//
LOCUS       DACGLW010000015        39691 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.015, whole genome shotgun sequence.
ACCESSION   DACGLW010000015 DACGLW010000000
VERSION     DACGLW010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 39691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39691
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.015"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            complement(82..639)
                     /gene="frr"
                     /locus_tag="IXT53_000873"
     CDS             complement(82..639)
                     /gene="frr"
                     /locus_tag="IXT53_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HAQ4904720.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(642..1364)
                     /locus_tag="IXT53_000874"
     CDS             complement(642..1364)
                     /locus_tag="IXT53_000874"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010761216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HAQ4904721.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(1500..2381)
                     /locus_tag="IXT53_000875"
     CDS             complement(1500..2381)
                     /locus_tag="IXT53_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HAQ4904722.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(2480..3262)
                     /gene="rpsB"
                     /locus_tag="IXT53_000876"
     CDS             complement(2480..3262)
                     /gene="rpsB"
                     /locus_tag="IXT53_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HAQ4904723.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            3621..4100
                     /locus_tag="IXT53_000877"
     CDS             3621..4100
                     /locus_tag="IXT53_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HAQ4904724.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            4317..5408
                     /gene="phnW"
                     /locus_tag="IXT53_000878"
     CDS             4317..5408
                     /gene="phnW"
                     /locus_tag="IXT53_000878"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HAQ4904725.1"
                     /translation="MYKLLTPGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSGSFGVESVLSSVIRPEDTVLICSNGAYGQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQEKDLL
                     FIVDAMSSFGGIPIRVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            5401..6186
                     /locus_tag="IXT53_000879"
     CDS             5401..6186
                     /locus_tag="IXT53_000879"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HAQ4904726.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIREP
                     MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEHYLFKDLVKHSTLKP
                     DTLETIERLRNAGINIGSTTGFTAPMMTIVAETAKKEGYSPDFMATSEDVEGYGRPFP
                     YMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEGSSLMGISYDEYQKL
                     EMGKKDRLKQSVALRFQQAGADICVNNLKELTEYILYADKNPE"
     gene            complement(6532..8223)
                     /locus_tag="IXT53_000880"
     CDS             complement(6532..8223)
                     /locus_tag="IXT53_000880"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HAQ4904727.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDEEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMRHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(8645..9592)
                     /gene="arcC"
                     /locus_tag="IXT53_000881"
     CDS             complement(8645..9592)
                     /gene="arcC"
                     /locus_tag="IXT53_000881"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HAQ4904728.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(9707..10726)
                     /gene="argF"
                     /locus_tag="IXT53_000882"
     CDS             complement(9707..10726)
                     /gene="argF"
                     /locus_tag="IXT53_000882"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HAQ4904729.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(10817..12046)
                     /gene="arcA"
                     /locus_tag="IXT53_000883"
     CDS             complement(10817..12046)
                     /gene="arcA"
                     /locus_tag="IXT53_000883"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HAQ4904730.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
     gene            12507..13208
                     /locus_tag="IXT53_000884"
     CDS             12507..13208
                     /locus_tag="IXT53_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HAQ4904731.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(13780..14796)
                     /gene="tsaD"
                     /locus_tag="IXT53_000885"
     CDS             complement(13780..14796)
                     /gene="tsaD"
                     /locus_tag="IXT53_000885"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HAQ4904732.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIVAGGVAANQ
                     GLREGLQDALATKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(14793..15257)
                     /gene="rimI"
                     /locus_tag="IXT53_000886"
     CDS             complement(14793..15257)
                     /gene="rimI"
                     /locus_tag="IXT53_000886"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAQ4904733.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLVEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(15264..15806)
                     /gene="rimI"
                     /locus_tag="IXT53_000887"
     CDS             complement(15264..15806)
                     /gene="rimI"
                     /locus_tag="IXT53_000887"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HAQ4904734.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTIEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(15790..16614)
                     /gene="tsaB"
                     /locus_tag="IXT53_000888"
     CDS             complement(15790..16614)
                     /gene="tsaB"
                     /locus_tag="IXT53_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HAQ4904735.1"
                     /translation="MYHCTSFAFGIFILRIKKYATMEGENVIAERIKYMITLGIDTAN
                     QTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAIDQLFADLQISPKDIDRIAVSDGPGS
                     YTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAANCIGVDKIIVPMFDARRKNVYAGAY
                     RWKENRLEIVLEDVHISASDLFTQLQKLNEAIYFVGSDCHKFFYEIKEILPSAMINTV
                     PAWDIPSGVTVAQLGALAEPVAEKQAFLPRYLKRVEAEEKWLETHTPGDESYVEKI"
     gene            complement(16703..17683)
                     /locus_tag="IXT53_000889"
     CDS             complement(16703..17683)
                     /locus_tag="IXT53_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ4904736.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPTDGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(17707..19191)
                     /gene="galT"
                     /locus_tag="IXT53_000890"
     CDS             complement(17707..19191)
                     /gene="galT"
                     /locus_tag="IXT53_000890"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HAQ4904737.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLADYPQVIAGVVKWPMSVIRLQSTEKKLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(19203..20192)
                     /gene="galE"
                     /locus_tag="IXT53_000891"
     CDS             complement(19203..20192)
                     /gene="galE"
                     /locus_tag="IXT53_000891"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HAQ4904738.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREVLGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            20441..20608
                     /locus_tag="IXT53_000892"
     CDS             20441..20608
                     /locus_tag="IXT53_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HAQ4904739.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(20670..22481)
                     /locus_tag="IXT53_000893"
     CDS             complement(20670..22481)
                     /locus_tag="IXT53_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HAQ4904740.1"
                     /translation="MKIGIIGAGPRGLSIAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNDLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSREGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGSMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLEIPLTYQDESFAEASRAFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPVIRTKELAALIDAGIFHLFEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(22478..22843)
                     /locus_tag="IXT53_000894"
     CDS             complement(22478..22843)
                     /locus_tag="IXT53_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HAQ4904741.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(23006..23401)
                     /locus_tag="IXT53_000895"
     CDS             complement(23006..23401)
                     /locus_tag="IXT53_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ4904742.1"
                     /translation="MVLWVINIILLLIVLAIIFWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(23419..24381)
                     /locus_tag="IXT53_000896"
     CDS             complement(23419..24381)
                     /locus_tag="IXT53_000896"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HAQ4904743.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRYLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACANLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(24381..24593)
                     /locus_tag="IXT53_000897"
     CDS             complement(24381..24593)
                     /locus_tag="IXT53_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HAQ4904744.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(24614..25312)
                     /locus_tag="IXT53_000898"
     CDS             complement(24614..25312)
                     /locus_tag="IXT53_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAQ4904745.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(25332..25874)
                     /locus_tag="IXT53_000899"
     CDS             complement(25332..25874)
                     /locus_tag="IXT53_000899"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HAQ4904746.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(26006..27010)
                     /locus_tag="IXT53_000900"
     CDS             complement(26006..27010)
                     /locus_tag="IXT53_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAQ4904747.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIGLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(27007..27996)
                     /locus_tag="IXT53_000901"
     CDS             complement(27007..27996)
                     /locus_tag="IXT53_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HAQ4904748.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVGKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(27993..28799)
                     /locus_tag="IXT53_000902"
     CDS             complement(27993..28799)
                     /locus_tag="IXT53_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4904749.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKITLLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            28965..29921
                     /locus_tag="IXT53_000903"
     CDS             28965..29921
                     /locus_tag="IXT53_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ4904750.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(29998..30516)
                     /locus_tag="IXT53_000904"
     CDS             complement(29998..30516)
                     /locus_tag="IXT53_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ4904751.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(30604..30753)
                     /gene="rpmG"
                     /locus_tag="IXT53_000905"
     CDS             complement(30604..30753)
                     /gene="rpmG"
                     /locus_tag="IXT53_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ4904752.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            30981..31427
                     /locus_tag="IXT53_000906"
     CDS             30981..31427
                     /locus_tag="IXT53_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HAQ4904753.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(31622..33517)
                     /gene="asnB"
                     /locus_tag="IXT53_000907"
     CDS             complement(31622..33517)
                     /gene="asnB"
                     /locus_tag="IXT53_000907"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HAQ4904754.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKLDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
     gene            complement(33842..34816)
                     /locus_tag="IXT53_000908"
     CDS             complement(33842..34816)
                     /locus_tag="IXT53_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAQ4904755.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNGTPENHFSNQISLNVYSRGMGGLGLPGDL
                     SSVSRFVKATFTKMNAASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAVNINKEDLDRHELVSYPVIKEQQINYVN"
     gene            complement(35152..35239)
                     /locus_tag="IXT53_000909"
     tRNA            complement(35152..35239)
                     /locus_tag="IXT53_000909"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(35205..35207),aa:Ser,seq:cga)
     gene            complement(35384..35950)
                     /locus_tag="IXT53_000910"
     CDS             complement(35384..35950)
                     /locus_tag="IXT53_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ4904756.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            36205..37536
                     /locus_tag="IXT53_000911"
     CDS             36205..37536
                     /locus_tag="IXT53_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HAQ4904757.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGVFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSVLSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGILLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLSGIREIFDSSGKLTT
                     DFIANSGIGATLINMGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            37502..37852
                     /locus_tag="IXT53_000912"
     CDS             37502..37852
                     /locus_tag="IXT53_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4904758.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(37944..38363)
                     /locus_tag="IXT53_000913"
     CDS             complement(37944..38363)
                     /locus_tag="IXT53_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAQ4904759.1"
                     /translation="MMTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVTFEPGCRNHWHVHTDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(38364..38708)
                     /locus_tag="IXT53_000914"
     CDS             complement(38364..38708)
                     /locus_tag="IXT53_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HAQ4904760.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVEWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            39058..39654
                     /locus_tag="IXT53_000915"
     CDS             39058..39654
                     /locus_tag="IXT53_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ4904761.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT        11518 a         8196 c         6898 g        13079 t
ORIGIN      
        1 tttttttaat ttttaaataa aagaaatata aataaaaaca gaggaagcgg aatgatccgc
       61 ccctctgttt ttatgatttt attaaacttc caataattct ttttctttat cagcagcgat
      121 cttatctact tctttaatgc tgttatctgt caatgtttgt acgtcttttt ctaatccgcg
      181 caaatcatct tcggtaatat caccattttt ttgttgtttt ttatattcat cgatcgcatc
      241 acgacggatg ttacgaatcg cgatttttgc attttctgct tcttttttca catctttagc
      301 taattctttt ctgcgttctt ctgtcagttg cgggataact aagcgaatga catttccatc
      361 attggttgga ctgattccga tatcacttgc cataatagct ttttcaacat cttgaagaat
      421 gctcttgtca aaaggtgtga tcattaaaac acgtgcttct ggaatttgga ttgatgccat
      481 ttggttcaat ggtgttggcg caccgtagta gttcacagta atacggtcca aaaggctagc
      541 gttcgcacgg cctgcgcgga tttgcccaag ttcacgttga agattttgtg ctgctttttt
      601 cattttttct tttgcttctg tcataattgc atctgccatt tttatttccc ccttacagtt
      661 gttccgatat tttcgcctaa gattgcacgg cgaatatttc cgtgttcatt taaattgaag
      721 acaagcagtg gaatatcatt atccatgctt agtgaacttg cagttgaatc catgacttgt
      781 aatcctttgg caataacttc cagatgagtc agttcttcaa attttactgc atttgcatct
      841 actttcggat cagcagaata aacaccgtct acattgtttt tagccatcag gataacatct
      901 gcgccgattt ctgccgctcg taatgctgct gttgtgtctg tcgagaagta cgggttgccg
      961 gtaccgcctg caaagataac gatccgtcct ttttctaaat gacgttctgc tttacggcga
     1021 atatatggct ctgcgatttg acgcatttca atcgaagttt gcacgcgtgt cggtacaccc
     1081 acgttttcca acgtatcctg taaagctaag gcattcataa ctgtagctaa catacccatg
     1141 taatcagctt gagcacgttc catgcccatt tgtgctccga tttgtccgcg ccagatattt
     1201 ccgccaccca caacgattgc catttcaacg cccagctcgt gaacttcttt gatttcttgg
     1261 atgatttctt tgattactgg tggtttgatt ccaaatcctg catccccagc taaagcttct
     1321 ccactcaatt ttaagactac acgctgatat ttcggtgtga ccatgttcat tcctccaata
     1381 ttttctattg ccattttacc atacttagat gatttcgaca ctctaagact ggtttttctt
     1441 gttttgctta aaaaaaggga gcgcacaaac cgtgcgttcc cgtctgttat tcaacagaat
     1501 tattttttca cttggctcat aacttcttct acaaagttat cttcgcgttt ttcgatacct
     1561 tcgccaactt cgaaacgtac gaatgtttta acagttgcgc cttttgatgc aacgtatttt
     1621 tcaacagtca tatcaggatc tttaacgaat ggttggtcca ccaaagcgat ttctgctttg
     1681 aatttcttca agcgtccttc taccattttt tcaacgatat tcgctggttt gccttcgttc
     1741 aatgcttgtt ctgtaagaac agttttttca tgttctaatt cagcttctgg aatttgtgat
     1801 tcgtttacat aacgaggatt gatagctgct acgtgcatag ctacgtctct tgcaactgtt
     1861 tcgtcagttg ttccatctag aacagtcaat acggcaatac gtccgcccat atgcaagtat
     1921 ccaccaaatg cagcattgtc gtctttttct acaacttcaa agcgacggaa gctgattttt
     1981 tctccgataa cttgagttgc ttcgatcaag tcagattcaa ttgtaccttt atctgttttg
     2041 attttcattg cagcttccat gtctgctggt ttgttttcag caacaagttc tgcaatttct
     2101 tttactaaat cttggaacat ttcgttttta gaaacaaagt ctgtttctga gtttacttca
     2161 acgattgctg cagtgttacc tttcacagca actgaagcca atccttcagc tgcaatgcgg
     2221 tcatttttct tcgcagcctt tgccattcct ttttcacgta aaagatcaac tgctttttcc
     2281 atgtcgcctt ctacttcaac taatgctttt ttcgcatcca tcattcctac gccagtcatt
     2341 tcgcgtagtt cttttaccat tttagctgta acgtctgcca tgtttaactc ctcctgtgtg
     2401 tttttctttt taaaaaaagc tgcttcaaag gagagtgagg cttgtcgccc agcctttgaa
     2461 acagctccat cattcaaaat tattctgctg aagaattgtc gccttcaaca acatctacga
     2521 tttcttcgat tgatgttgct gtgttttctt ctgtgaaatc ttcttcaact acttgatctt
     2581 caccttggtt tccttcgatg aaagcatcag ccatttttga agtaatcaat ttaacggcac
     2641 gaattgcatc gtcgtttgaa gggattacta cgtcgatttc atctggatcg cagtttgtat
     2701 caaccatagc tacgattggg atatttaatt tgtgtgcttc ttgaacagca atacgttctt
     2761 tacgtgggtc aacgatgtac attacatctg ggattcttgg catatcagcg ataccgccta
     2821 agaatttttc aagacgttca cgttctttgt tcaaaccagc aacttctttt ttaggtagaa
     2881 cttcaaaagt tccatcttct tccattgcat tgatttgttt caaacggctg atacgttttt
     2941 ggatagtatc ccagtttgtc aatgttccgc ctaaccaacg atggttaacg aagtattgtc
     3001 ctgcacgagt tgcttcttct ttgattgctt cttgtgcttg tttttttgta cctacgaata
     3061 atgctacgcc gccttcttca gcaacgctct tcatgtaatc ataagctgca tctactaatt
     3121 taactgtttt ttgcaagtca atgatgtaga ttccgtttct ttctgtgaag atatatttct
     3181 tcatttttgg attccagcga cgagtttggt gaccaaagtg tacgccggct tctagtaatt
     3241 gtttcataga aattactgcc atgtttgttt cctccgattt ggttttattt ttcctcttct
     3301 tatcttctac cttgccggca aactttttca agcacctacc gacaatccaa taagaatgtg
     3361 gatttgctga tgcataaagc accctcagct attatacaga caaaagatcc gtatcgcaag
     3421 agcaaatata cttttctgct caacattcaa tagtttacct gaaagataaa aagaattcaa
     3481 gaaaaacagt agatttctct ttgtttcttt attatccttt ttccataaaa taattttagg
     3541 tataaaagac gaatattatt gggcaatcct tctttttttt gttatgatac atgtgaaatc
     3601 tttcgtaaag gagaaagaaa atgcgcaaag ctcaaagaca tttgctgatt aaacaagtca
     3661 ttgaagaatt cccgatccgt acgcaagaag aactattaaa caaacttggg cattatggcg
     3721 ttaccgccac gcaggcaacg atttctcgag atatccgtga tctaaaaatc gtgaaggcac
     3781 ctgatgaaaa tggcgtttcc cgcttcgtat tgtttcgcga aaaaggaact cctgagacta
     3841 ggactgagga agaggatcgg ctggttcaaa tgattgaaga cattgtattg aaagtcgaac
     3901 gagtccactt tttgacgatc gtcaatacct tgccagacaa tgctcattta tttgcatctg
     3961 ttttagatga gatcaaacct ccctctattg tcagtacaat tgctggattc gatacgatca
     4021 tggtcatctc ggaaaatgag gaggacgcaa aacgagtaga agaatttctt caccatccaa
     4081 ctgaagtatc acttttttaa tcagagcacg tattcctaat atggaatgcg tgttttttta
     4141 ttttcaatat tttccttatt aaataaacct ttacactctt tacaaaaata catcattttt
     4201 tattcaattt tttcaacacc ttgattgaat cacaataatc atcatttata ttggatgtta
     4261 acaagagcag cttcttgctc tttgtgtatt tcaaaaaaga aatgaaagga aatcatatgt
     4321 ataaattatt aactcctgga ccattgacga cttctgaaac agtgaaaaat gctatgctga
     4381 tcgatcattg tacttgggat caagaataca aagaaattac acaatggata agaaaaaaat
     4441 tattggatct tgctcaagta tcggaaggaa tatacacaac ggtcttaatg caaggtagcg
     4501 gctcctttgg ggtagaatcg gtattaagtt ctgtgatcag gccagaagat actgtactga
     4561 tttgttcaaa cggcgcatat ggacaacgaa tgatcaacat gtgcgaatgt ctgaaactga
     4621 actataccgt ttatgaggta tcggaacatc aaatcctcca agctcatgtg attacacagc
     4681 tgattgaaga gaatccctct atctccgcac tagcaatggt ccatagcgag actacttccg
     4741 gtattttgaa taatttggaa gagattgctg ctgtcacaca agaaaaagat cttttgttta
     4801 tagtggacgc gatgtcaagc tttggtggga taccgattcg tgtagccgaa ctagggattg
     4861 attttttgat ttccagctca aacaaatgta ttcaaggtgt tccaggcttt tcttttgtac
     4921 ttgctaataa agcggaacta gaaaagcgaa aaaatgtgag tcgaagtttg tcacttgatc
     4981 tatatgacca gtaccgcgtg atggaaaacg agaatggcaa atggcgtttc acttccccta
     5041 cccatacggt tttggctttt caacaagcgc tgaaggaact agaggcagaa ggaggaatcg
     5101 aaactcgata ttcacgttat ctcgccaata atcatgtact acgacaaaaa atgacagaga
     5161 tggggttcca gcctttcatt caagaatatc aggggccatt catcacctct tttctttacc
     5221 ctaaggactg gcgatttgat tttaatcatt tttatcagta tttaaagcaa aacggatttg
     5281 tgatctatcc cgggaaaatc agcgatcatg aatgttttcg tatcggaaat attggcgaga
     5341 tctatccgga agatattttg gaactgacag caattatcaa agactataag gagaacgtta
     5401 atgattaaaa ctgtaatttt tgattgggca ggaacaactg tggactttgg ctgtatggct
     5461 cctgtccacg cttttagaaa tgcttttcta gaaaaaggaa tccaactgac tgacaaagaa
     5521 atccgcgaac cgatgggaaa attaaaatgg gatcatatcc aacagcttct ttccctccct
     5581 tctgtcaaaa aacaatggat agctatttat ggacaactac ctcaggaaaa agaggtggag
     5641 gaattgtatc aacaatttga acattatctg tttaaagatt tggtcaagca ttctaccttg
     5701 aaacccgaca cattggaaac gatcgaacgg ttgagaaacg ctgggatcaa tattggttcc
     5761 acgactggat ttactgctcc tatgatgaca atcgtagcag aaaccgcaaa aaaagaaggg
     5821 tattcaccag acttcatggc aacttctgaa gacgtcgaag ggtatggtcg accgtttcct
     5881 tatatgatcc ataaaaacat gcagtacttc aaaaacaagt cggttgatga agtaatcaaa
     5941 gtcggagata cggtctcaga tatccaggaa gggaaaaatg ccggagtgtt gacagtaggt
     6001 gtaattgaag gtagttcgct aatggggatt tcttatgatg agtaccaaaa attagagatg
     6061 gggaaaaaag atcgtttaaa acagtctgtt gctcttagat tccaacaagc tggcgctgat
     6121 atttgtgtaa acaatttaaa agagttaaca gaatacattt tgtacgctga caagaatcca
     6181 gaatgacttt tatacaaaat cagaggtcgt gaaaaagctg tactgcatca agtaataaga
     6241 acagccaaac aaaaatagct cctcatattt ttcgtttggt taggcttatt accgcagatg
     6301 caagcttttg aacaccgttt attcgaaaaa aaaagggagc tgtgacacga cttttgtcac
     6361 agtcccctta tttacaaaca aacagtgttt aagagacaac ctctccacta aacggtgttc
     6421 attcagctga ctctcggtat ttgctcaaaa aacgataaaa tttgaaaagc tattttctct
     6481 tttgtttctc taatactcgg atcagaacgc tgatttcaca ttctcttttt cttacatctt
     6541 atctggtgca tgaagtccta gcaaacgaag gtcttctttc aataatacag tcactgcata
     6601 aactagagct aagcgtgctt ctttttgttc atcatcagct aaaatcttcg tatgagcata
     6661 gtatttgttg aatgcttgtg ctaatttgat tgcatgtttc gcaatcactg acggttcgta
     6721 tttttcccca gcagacaaga ccgtttcagg gtatttttga actaatttaa ccacttccca
     6781 gctggtatcg tcattcaatg catagtctgc tgtttctgaa gggacaaatc ctgctttttc
     6841 caacaggctg actgcacgag catgtgtata ttgcacataa ggtccagttt ctccttcaaa
     6901 tcgaacaact tcttccaggt taaagtcaaa tgtattcagt cgatcgtttt tcaaatcgtg
     6961 gaaaatgact gccccaaccc caacttgttt agctacagca tctttgtttt ccaaatcagg
     7021 gtttttctcg ctgatttgtt ctttagcaga ttggattgct tcatttaata cttcttctaa
     7081 taagacgatc tttcctttac gagttgataa ttttttacca ccttgcgtga tcaaaccaaa
     7141 tggaatatgg cgcatatcgt cagaccagtc aaagcccatt tccttcaaga cagctttcaa
     7201 ctgtttaaaa tgatagcttt gttcattccc tactacatat agagattgtt tgaagtcgta
     7261 agtacgttta cggtacaatg cagcagctaa gtcacgtgta atgtatagtg tcgcaccgtc
     7321 tgacttctta atcaacgctg gattcaggtc ataagcagat aggtcaacga tctctgcacc
     7381 tttatcctcg ttcagcaaat gtttttcttc taaaagtttg acgatttcat ccatcttgtc
     7441 attgtagaaa gcttcaccgt tcaacgaatc gaaacgtact tccagcagat catagatttt
     7501 attgaattct ttcatcgatt catcacggaa ccattgccat aattgaatag cctcttcatc
     7561 gccttcttcc agacgtttga accaagcacg agcttcttca tttaattctg gttctgtttc
     7621 agctacttca tggaattgga catataaacg aagcaattca ttgattggtt ctgctttaac
     7681 agcttcttct gtaccccatt ttttgtaagc gacgatcagt tttccaaact gtgttcccca
     7741 gtcacctaaa tggttgatgc ggattggctg ataaccgatt ttttccataa tgaaaccgat
     7801 cgaattaccg ataactgtcg aacgcaaatg tcccattgag attggtttgg caatattagg
     7861 agaagacatg tcgattggta ctgttccttg attcccgata ttgctatccc cataatgttc
     7921 tttttctttc actactgttt gcaaaacttt tttgctgata agttctttat tcatgaaaaa
     7981 gttcaagtaa ggtccgacaa cttcgatttt ttcaaagttg gcagaatcga ttttttctgc
     8041 taaatctgca gcgatttgct gtggcgcttt tcggtaaact tttgctaatg aaaaggctgg
     8101 aaatgcaaca tctccgtgtt cagctgattt tggattttct agtaattgtt cgacttgttc
     8161 cagtgttaaa tcatctttga cagcgtcata gacagctttt gcgacaagat ctttattatt
     8221 catctttatt cctcctaaag tgctcccttg attgttaaac aaaaaaaatc tctagcgacg
     8281 gcatacttag tatgccatca ctagagacga gatttcccgc ggtaccactc caattgtcct
     8341 aaaaaaggac cagctccatc tgatatcggt agacgaccgt ctgtttaaaa cagaattctc
     8401 caagtgcgct tcgttgtatc ttcttcattc ggcttccacc acttccgaat tcgctagcca
     8461 agaatgatac aagtactctc ttgttcaaca aatcttagtc ttattaaact ttcttgaatt
     8521 atatcagaaa aaaaaacgaa aggctagtcg tttctgataa aaaaacaatc cagatggcct
     8581 aaaggagaag aaataccacc tggatagcta taaatcattt cgtaaatacg aaagacttaa
     8641 caaattaatc agctacgata atcgttcctt cttcagaagc taaaagattc tcgatatttt
     8701 ctaaagaagt gatgattgcc ttagcgtttg gttttgcttc aacaaatgca atagctgcct
     8761 ctactttcgg aagcatgctt cctggagcaa attgcttttc atcaatgtat tgtttcattt
     8821 ctgaaacagt aacagtttct aatttctttt gatctggttt ttggtaattg acatacacat
     8881 tgtctactcc tgtcaatacg atcagcaaat ccgcatcgat gatttcggct aatttttctg
     8941 aagcaaaatc tttatcgatt acggcttctc gtccttctaa tccagtagct gtttctacaa
     9001 caggaatccc accgccacca acagagactg tgataactcc ttgatcgacc agcgtttcga
     9061 tgacacgtgc ttctttaatc gagatgggtt taggggaagc gactactttt ctccatccac
     9121 gtccagcatc ttctttgaat gtcacagtgg agtcagcatt catttgttct ttggcttctt
     9181 cttccgtgta gaatggaccg actggtttac ttggattttt gaatgaagga tcattttcat
     9241 ccacaataac ttgagtgact aaagaaacca catctttatc gatccctttt tccttcagta
     9301 cttcgcccat ggcattttgt aaccagtaac cgatagatcc ttctgtcatt gcaacacatg
     9361 tatctaaagg cattgccggt gttttctcag aatcagcagc ttgctgctga atcaataggt
     9421 taccgacttg cggaccattt ccgtgggaga tgatcaattc atctccttgt tcgataaatt
     9481 tcactaaata ctttgctgtt tccattaacg cttcttgttg ggcttttgca cttgcatccg
     9541 tagacaagat tgcattaccc cctaaagcga caactacttt tcgtttggcc atttcattcg
     9601 tcctttcttc agttctaccg aattttctag ataacaaaac gagccagcgt tttgtgattt
     9661 tccctcaatg ctgactcgtt ttttcgtgag atgaatgtgg ttatgcttaa acgcgaggga
     9721 taaacaagtt acctaatgtt gctgccatga tcgctttgat tgagtgcata cgattttctg
     9781 cttggtcaaa ttgacgagca tatttgctgc ggaagacttc atctgttact tccatttcag
     9841 tgatgccaaa gcgttctttc atttgttcac cgtaaacagt gtttgtatca tggaaagctg
     9901 gcaagcagtg caagaagatc aaacgatctg tgttgtgtgt tttttctacc atttccatat
     9961 tgatttggta aggtttcaac aatttgatac gttcttcgaa tttatcttct tcacccattg
    10021 atacccatac atcagaataa aggacgtctg ctccgtctac acctttatca acatcgtcgg
    10081 taatcatcaa ttgtgcaccg gatttttcag caaagccttc tgccattttg acgatttctt
    10141 cttcaggttg taattctttt ggtgcaacga tacgcatatt cgctcctaaa attgcacctg
    10201 tgactaataa agagttagcc atattgtttc gaccatctcc gcagtaagca acagtgatac
    10261 cttctactgt tccaaagttt tcttggatag ttaaaaagtc agcgatcatt tgtgttggat
    10321 gccattcatc agtcaagcca ttccaaactg gaacaccaga gaattctgct agttcctcta
    10381 ccattttttg actgaatcca cggaattcga ttccatcaaa catacgacct agtactttag
    10441 ctgtatcttc tgtggattct tttttaccta attggatatc atttgcacct agatattcag
    10501 gatgtgcacc tagatcgatc gctgcagtag taaatgcaga acgagtacgt gtagatgttt
    10561 tttcaaataa aagcgcaata ttttttcctt ctagatagtg atgtgggata ccacgttttt
    10621 tcaagtcttt caagtgttct gaaaaatcga tcaaatattg taattcttct tttgtaaagt
    10681 ctttttctgc taataagctt cttccttgga atactgattc tttcatatta aacattctcc
    10741 tttagattgt ttttatttaa gattaaagag gaaatcccga cggcagctga cagtaagacc
    10801 gccgtcagga aacaaattac ttcaagtctt cacggacaag tggttggctc atgcagcgtg
    10861 gaccaccacg gcctcgtgac aattcacttg aattgatttc taacactttg atgccatagc
    10921 tacgaagcaa ttcgttcgat acataatttc ggttataagt gactacaaca cctggagcga
    10981 ttgccaatgt gtttgatcca tcattccatt gttctcgagg tgcaacgatc tcatctccat
    11041 taccagttgg gatcagtaca agatcatcta atccaagagc agctttcatc gtttcgtgta
    11101 agttatctga atgagtaatc ttgatatcat caccatctgg ttcgattgta aagacatcca
    11161 tcttaccgtc tttactttga attgctggat ggatagtgaa tttatcgtga tctaccattg
    11221 taaatacggt atccaaatgc atcattgcac ggttgtttgg aattttgatt gctaacactt
    11281 tttcaaatcc tgaatttttt gcgaataggt ggcgtgctaa tttttctaaa gctttcgcat
    11341 tagttcgttg agaaatccca acagctacta ctttatcgct taagatcaat tcgtctccgc
    11401 cttcgatatg atctggattt tcacgatcca accatacttc gattccttta tttgcaaatc
    11461 ttggatgatg tttcaaaata tattctgtaa acatcgattc acgacgacgt gcttcaaaag
    11521 tcatcttatt gactgtcatc ccatttccca ttgatgcgga agggtcgcgt gtaaagtaca
    11581 agtttggcat tgggtccatg taaaatggat agtcatcgtc tgctgacaga tcgtatagac
    11641 tgctagaacg tacttcaata tcttttgtac gaaccccagc catgatttta tctaccattt
    11701 cttgcgtttc cattgataga aggaactcat gaagaccctc tcttacagca ttcgatgcaa
    11761 tatgagattc atccaacatt ttattcaaga attgttcttt gacattgcct gcgtcaatcg
    11821 cttctgccgc taatttttcc aagtaaagag tctcgacgcc ttcattttgc aatgtctttg
    11881 caaagttgtc atgttcttct tgagcaatcg gaagatatgg aatatcatcg aacaacagac
    11941 gatccataat atctggtgtc aaattttcca cttcttgtcc aggccttttc agtaaaactg
    12001 tctttaattt ccctatttca gagaaaacgt gaataggttt atccatgttg tgttgcctcc
    12061 ttctttttga ttacatgtat aggatatcct ccttttgtaa tcactttcat aattttgtct
    12121 cattcaaaat gagacattcc tcacattaat attcgataat taaataatta aacgtttttc
    12181 tatttttaag cgtaatttat tcatttttta ttctgtatac actttcaaaa aaatatttat
    12241 tcattgaaaa aaattttttg aataaattaa aaaaataaat tatattagcg ttgttagcgt
    12301 ttactatatg tattattgtt gttttttata cacaaatcat tcgcttcaaa acaaattatt
    12361 caataccatt gtatattttt tacataataa caaattatat ttgtaaagta ttaaaacttg
    12421 tttctaatgt tggtatgtta aaactttaca aattctttaa aaaaagtgac aataaatata
    12481 gtgaacaata aggagaggac tttttaatgc gtgttaataa aatcgatttt cacttttcaa
    12541 aactcagata tcaacctgat tttgaacatt tctcggatga agagttccaa cagatcaagg
    12601 aacatactgt tcttcgttct tataaaaaag gacagatcct tttcgacgat ggtgatccca
    12661 gacaacggtt gtatatcttg accaaaggac tcgttcgttt agaacgttat gatgaaagtg
    12721 caacttatta ctactatgac tatgtgaata caaataacct ttttcctatg tcaggaatgt
    12781 ttggagaaga gacctacact tatacagctc aagcgatgac tgatattgaa acattctacc
    12841 ttcctgtttc ctactatgaa aaattagcac gaaaaaatcc cgaacagttg atctatatga
    12901 tacaaaaaat ttctaaagta gttgaaatac atgaattgcg tatccaaatc ggattgacct
    12961 ctagcgcctt tgaccgggta aaacaaaatt tattcatttt gaaagaagaa ttgggcgtac
    13021 caacaaaaga tggtcatatc accattcctt atccgatcac cttaaaagaa ttagcagtga
    13081 atagcggcac gacacgtgaa actgctggac aagtaatcaa acaactacgt gattccggac
    13141 tgttagatta tgacaaaaag cgttttactt tctgtaagac agctactact ccagctatga
    13201 gagaataacg cgaacaaaaa taagcagtga ccctgatatt tatcagggtc actgcttatt
    13261 ttcctcccta aaacttctga aagatccggc agttttagat cactgggcaa gactcaaaga
    13321 ttttctatgt gtgtttcagt tatgtaatat tcactgtttg caaaaaatac tgatagatta
    13381 actagaggaa gcgtcttgtc ctcatccgac ctagattatt cgatattcct cgacaataaa
    13441 cgattaatca gtgagggcaa tcagaggaac ggataatgat gaataagctg taaaaaatag
    13501 ggctttgaca aaagcgtgtc acagccctta tttcgattaa acggtgtgta cctgtcaact
    13561 cttcggtcac aagtcataat gttcataaac atgaggagct gttttttgaa attgttcctt
    13621 gtgaatcaga gttaaacgac aggcttacga actcttgatt aaacggcgtt caaaagcttg
    13681 catacgcggt aataagaaca gccaggtcaa aaacatgaga agccgttttt cctgggctgt
    13741 tcttattact tgatgcaggg cagatttttc acaacctctt tattcagata aaaccaagct
    13801 tggatcagca ttcaactgat agctagcaaa atttcttttt tgcatttcca catgtgctgc
    13861 tgctccaatc attgcagcat tatctccaca aagtcgaaga gggggaatca ctagttctac
    13921 ctcagggagt ttagttgcca aagcatcctg caatccttca cgcaaccctt gattggccgc
    13981 tacacctcca gctacaatca gttgcttgac tggataattt tgacaagctc gaagtgtttt
    14041 agtaatcaat acatcgatca cacttgcttg aaagctagct gctaaatcat ttttgtctag
    14101 atcttctcca cgctgttgtg cattatgcac caaattgata aatgcacttt ttaatccgct
    14161 aaaactgaaa tcataattat cttcatggat catcgctcgt ggaaaatggt aattatcttt
    14221 tccctgatga gctagctgat cgatttcttt tccgcttggg taagaaagac ctaatactct
    14281 tccaactttg tcataagctt ctcctgctgc gtcatctctt gtctccccaa tgatttcata
    14341 tgatccatct tcttgcatat agactaattc tgtatgtcct ccgctaacaa gaagtgccat
    14401 caatggaaac tggaatggtt taaccagacg ggctgcatag atatgccctg ccatatggtt
    14461 tacaggaata agcggcaatt ggtgtgccca agcaaaagcc ttagctgcag aaataccaat
    14521 cagcaacgag ccgacaagtc ctggtccata agtgacagcg actgccgata aatcttctgc
    14581 cgacacacca gcttctacca gtgcatcttc caagcataag gtgatttgtt ctacgtgatg
    14641 gcggctagct acttcaggaa ctacgccacc aaaacgttta tgactgttta tttgagaagc
    14701 aacgatgtta gataagatct ctgttccatt tctaacgacc gctacactcg tttcatcaca
    14761 acttgattca atcgccagaa tcaattcttc attcatttca tcgtctccgt ttttaacttt
    14821 ctgctcataa tgatcgcatc ttccgtcgga ttgcgataat atgatttccg tttacctaat
    14881 atacgaaact tttctgattc ataaagtttt cttgctgctt cattagatag acgtacttct
    14941 aaaaaaacat gcagcgaatc ttttatttgt tccgtaatca gtaattgtaa caaatgccgt
    15001 gcatgccctt ttccttgttc tgaaactgca acagcaatat ttgtgatctc gacttcatct
    15061 attactttcg agtatccaat aaagccacag atctggttat cttcaactaa aagcagataa
    15121 tcagagtgag gctgttggat gtctgccaaa aactgcattt tcgtccaagg tgaaccatgt
    15181 ggataagcat tttcgctaat catccatacg cattctgcca agtacgcttc atcaaaatct
    15241 ttttttctgc aaatcaacta atctcactca ttttctaaaa gttttatcat atccaacgca
    15301 tcttcttttg tttcagtgta atatcttttt ttgatcgttg ttgcttgaaa acctaacttc
    15361 cgataaagtc tttgtgcatc ttgattactc atacgcactt ctaatgacat cgtctcacag
    15421 cgattcatta tagcaaagtt ctcgatttcg tcaataagca gcgaaccgat tcgtttacgc
    15481 tgatatgccg gtcgaacggc aatattcgtg atatggcaat ctaaaccgaa cgctcttgaa
    15541 ccgataaaac caatcattct tccattatca aagataccta gatatagatg aggaagcggt
    15601 gacttcaatt ctgatataaa ggcacttttt gtccatggca attcgccatt atagacttct
    15661 cgttcgacat ccagcagctc tttgatatct tctgtagtca attctcttag tacgtaacga
    15721 cgatcgattt gttcgattgt tttttctgta taaatcattt tatcttgaaa aaagcttctg
    15781 gcatacgctc taaatttttt caacataact ttcgtctcca ggtgtgtgcg tctccaacca
    15841 tttttcttct gcttctaccc gttttaaata gcgaggaaga aaagcttgtt tctctgcaac
    15901 tggttcagct aaagcaccta attgagccac agttacacca cttggaatat cccatgctgg
    15961 aactgtatta atcatcgcac taggcaatat ttcctttatt tcatagaaga atttgtgaca
    16021 atcactcccg acgaaataaa tagcttcatt cagcttttgt agttgtgtga acaaatcaga
    16081 agcagaaata tgtacatctt caagtacaat ttctaatcta ttttctttcc agcggtatgc
    16141 cccagcgtat acattttttc ttcgagcatc aaacatgggc acaataattt tatccacacc
    16201 gatgcaatta gccgcgatgg ttttcagact agatactcct actagctctt tatctaatgt
    16261 ataagcaatc gtttttgctg tcgttacacc tatacgaagc cctgtataag aaccgggacc
    16321 atcagaaacc gcgattcggt caatgtcttt tggacttatt tgaagatctg caaacaattg
    16381 gtcgattgca ggcatcagtg ttacactgtg gttgcgtttg atatttgttt gtatttgtcc
    16441 aagaatttgt tcgtcttcta ccacaccgat cgccaaagtt tggtttgcag tatctattcc
    16501 taatgtaatc atatatttga tcctttctgc tatgacattc tcgccttcca ttgtagcata
    16561 cttttttatt ctgaggatga aaataccaaa ggcaaaacta gtacaatgat acatgattat
    16621 ctgctggcgg gcaaattttg ccccttcctg aaacacaaaa aagctgcggt atcacttcat
    16681 ttccccacag cttttccatc ttttatttcg ttgattctcg aataatcaac tctgtttcca
    16741 caataatctt tcttgaaacc ggtgctttgg aattacttaa ttgaagaatc gtctccactg
    16801 ctagctctcc catccattcg gtataaactt ttacagtaga aagagagggc gttacatatt
    16861 tcgctacact gacatcattg aaacccatca cagaaatatc ttctggtacc actaaacctt
    16921 tttcttgaat cgctttcagt gcgccaatcg ctaatgcatc attagctgca aaaagcgcat
    16981 caggaacatg cggattcgtt tttaaatact gcttcactgc ttgatatcct gactcgacag
    17041 aaaaatctgc ttcgataatt ggaccttcgt cgtagccttt ttctgccaaa acttgttgaa
    17101 atgccataat tctgggatct tttagccgct gatggttttt cttcgtatat tctgttccag
    17161 caagcatagc gatatgttgc gctttatggg agacaaaatg ttcaatgact ttttgaacac
    17221 ttgacttaaa gtcgacaact agagagtcga ttccttgaga agtgccgtca gaatctacca
    17281 aaagcaaggc attttgggat cctttcattt ctaataactg agcttcgtcg aattttccta
    17341 gtgcgatcgt tccatctgtc ggaagatcac taatggaatc ccacgtttct ttcaataaag
    17401 taacgtttgc ttcttctgcc tttttttcga ttcctagacg aatagataaa taataaagat
    17461 cttcaagctc ttccgtatcg tcgtaccact ggatcaggcg aagcgtttgc ttagtatttg
    17521 ctgcattttt acgatgtttc gtataattta agcgttctgc tgcttcaaaa attttttgct
    17581 ttgtggctaa gccaacggaa agatcaggat catagttgag aactcttgaa acagtagcag
    17641 gagaaactcc tgccaactgc gcaatgtctt taattgttgc catttgtctc tcctcctttt
    17701 ttacgtttat aaaattgcag taaatcgttc aaacgctgcc tgtccttttt catctcgctt
    17761 gaatactcca gcgtcttcca gtacacgtgc aaaaacctga cctacagatt tttgtacgac
    17821 atcggtgacc gtatctttcg tcaaatcagg atgttctttt tttaattgtt ctgcccacgg
    17881 acgatgatat tcagccatct ggttttcttc tcctaataag aaatgctcta cttctactag
    17941 ttctggtttc aaacgaggtg gtaaaacagc aagccccatt acttcgatca acccaatgtt
    18001 ctcctttttg atatgttgga catctttatg tggatgaaaa atcccatcag gatgttcttc
    18061 tgaaacattg ttgtctcgaa ggacaaggtc caactcaaag taattgtccc ttcttcgtgc
    18121 aattggcgta atcgtatggt gtggtgtacc atctgctgaa aaagctctga tagatactgt
    18181 ttcatccgaa tatgctttcc acttttccaa tactgagtta gctgcttcaa tcaacaattt
    18241 tttctctgtt gattgcaaac ggatcacaga catcggccat ttcacgactc ctgcgatgac
    18301 ttgcggataa tctgctagtt tgataagttt ttctatcggt gcttttgcca taggaaattc
    18361 atgacgtcct gcctgataat gatcatgaga aagaatcgat cctccaacaa tcggcaagtc
    18421 agcattcgaa ccaacaaagt aatgaggcaa gacttcagta atccgaagca gtctagcaaa
    18481 tgtttccttt gtgattttca tcgggcggtg ttgttctgat aaaatgatcg aatgttcatt
    18541 gtaataagca taaggagaat attggaatcc ccagctttca ccgtctagat tcattcgaat
    18601 aatccggtga ttggtgcggg ctggatagtt caagcgacct ctatatcctt cgttttccat
    18661 gcaaagcatg caggtaggat agtctacttt taccgcctcg cgttctgctg cgatttgttt
    18721 tggatctttt tctggcttag ataaattgat tgtgatttcc agttcaccat actccgactc
    18781 tgccggaaat ttgatgtttt ttgcgatggc tcttgttttg atgtagtcat ttttcttact
    18841 taaattaaag aaatactcag ttgcttcctc tgggcttttc gaatagtgct gtgcaaagaa
    18901 tgcattgacg actgaaggtg gcggtgtgag aaaatccatt aattgcgctt caaaaatctc
    18961 ttgttctgct ggcaattctg aaagaacacc attttctctt gccttagcga caagctgatc
    19021 caataagtct acagattctt ccttaactgg tcttacttca atgctttcta gagcatcttc
    19081 tcctaccatc cctaaaagac gattctgtaa atataagcgg tccatttcca tccatccgcc
    19141 agcttcgatc gcaagtgtgg caaaatcaca aatcgtttga ctgattgaca tatctcattc
    19201 ccctattctt cataaccatt tggatggctg acatgccaat cccatgctgt tttgataatt
    19261 gctttgatat ctgtgtattc aggtttccaa cccagaacct ctctcgcttt ttcacttgaa
    19321 gcgactaatc ggctaggatc tccagctctt cttggtgcta tttctgctgg aatttctttt
    19381 cctgtcactt ctctagctgc ttctagcatt tccttgactg agtatccttt gctgctgcct
    19441 aaattgaaga agttgctttc atttccttct ttcaagtatt ccaaagctaa aatatgtgca
    19501 gcaatcaaat cttctacttg aacataatct ctgatacatg ttccatctgg tgtatcataa
    19561 tcgtctccat aaacagccaa tgcttttctt tgaccaagag ctacttgtaa aatgattggg
    19621 acaagatgcg tttctggtgt atgatcttct ccgatagatg catctgcttt tgctccggct
    19681 acattgaagt agcgcaaagc gacatagcgc atgccatatg cttggtcaca ccacttcatc
    19741 attttttcca tcatcaattt actttcacca tacggatttt taggattagc tggtgtatct
    19801 tctgtgattg gagactcttt tggttctccg taagttgctg cagtagaaga gaacacgatt
    19861 ttattcacat tgaactcgtg catgacttca agtaagattt gcataccgta tacgttgttg
    19921 ttgaaataca tcaatggttt ttcgacagat tctcctacta aagagcttgc agcaaaatgg
    19981 attactcctt caatcggttc cttttcaaat acggaacgta gaaattcttt atcccggatg
    20041 tctccttcat aaaaatgagc atctggatgt actgcttgtt tgtgacctgt gagcaaatta
    20101 tctaccacag ctacttgata ccccttttga actagctggt ctacagcgtg cgaaccgata
    20161 taaccggctc cgcctagtac taaaattgtc ataaaaaaca ctcctttgat tagtaaaatt
    20221 gtgtttgttg attatatata atttaaaaaa acgaagttta cttttatggt gaaaacaaac
    20281 aaagtaaaat ttcgtaaatt catatggtaa atttataata aaacatttag taaacatttt
    20341 caaggatttt ttgcaaaggt ttacaaacaa acgtaaaact agcctttttt tctcttttgt
    20401 tccataatag atataacaaa taataaatga ggtggattca atgaaaaaat ttgtatctgg
    20461 aatattagta ggaagtctgg caacagccgc tgccgttgct ggtttggtcg cttctgtgaa
    20521 aaaaacagtg atcgatccaa ttgacgaaaa agaagcaatg atcgaagaaa accgtaaaaa
    20581 agcaatgcgc aaacgcgttt ctcgttaata attatttaaa aacacgcatg aagacttgtg
    20641 aaagaacaga taagtcatca tgcgtatttt tatttgtcag tcaatataag ttgtgctatt
    20701 ttatctgcct gtctcagatt ccatggatct gtataaggtc tagctactgc tgcagttagc
    20761 caatccacac cttcaacagg tattccatag caataaaagt cagactggat ttcccctgaa
    20821 gcatcaatca cgcgattcgt acctcgctca acatctatag cttctgaatg ataacttcct
    20881 gtattatctg aaaatgaaaa agggcgaaca agtttgtttt ccttcaaatt ttttatgaca
    20941 ggattacttg aacgagaaaa gcttgtttgt ggcaatcgag cttcaatcaa atagcgactg
    21001 ctgatttctt tatcttgtag agaaataaca tagcggttgt gcttttgctg aatatcaaat
    21061 ggcggttcga acaaatgaaa aatacccgca tctatcagtg ctgctaattc tttcgtccga
    21121 atgactggcg gaccgatcgt taaaaagcta tttagatgag taaaccatcc ccatagtatt
    21181 tctttgtatt cctttgcaga aaaaacgtgc cattcaatag cctgccggat tggatcacgc
    21241 cagtctttta atgcatcaaa tgttgaagca aatgctcctg tgcaattgcc tttttccgct
    21301 tcttttatct gcccttcaat aaatgctcgg ctagcttctg caaaggactc atcctgatag
    21361 gtaagaggaa tttctaaata cgaccaatcc catctataag ggattagtgc tggatgcttt
    21421 ccgagacact cctctgaaga gagggagaga aaatcatgct caaaattttt ctttggaatc
    21481 ggcaaatgat attcttcgat gattttcttg taataaaaca actcaacgtc ttttttcaga
    21541 tagtcaaaaa atgtttcgcc agtcaaaaat cccgactgat gccattgctc caggttttct
    21601 tttgttagta accgtggcca tgccattgat ccgccttgct tttggtttct tcctcgaggg
    21661 taataaggga gcccccttct tgatccactg tatacaatcg gttccttgcc tgatggatga
    21721 tagacaagtt ttcctttttc gttctcgaag cgcccttctc ttgatactgt aaacaatccg
    21781 acataatcaa aaaaacttaa gcctagacct cttaaaaaga cattttcttt aggcggtatc
    21841 ttcgaaagat cgacatctgc aggattagaa ggaagctgat acagtaaatc atttttcttt
    21901 gcgtagtcta cccacactct ttcttcttct gtctcatcat tttcccagtg accgcttgct
    21961 aaaacaagct gatcgataga atagctttgc tgatctgtct gtaaaagata accatctttt
    22021 cttttgctga ctttttttac taaagaataa acaaactgaa tagaagctgg aaattcttct
    22081 tgcaactttg agaaaaacca gcgttgatag agtccgtata aacatcgagt agattgctga
    22141 tctttttcca gacgattgat ttcttctata attagttgct tatttttggt taccgtctgt
    22201 tcgatataat ctgtagcagg tcctttactc cattgaaaca agttgggtcc ctgtgttacc
    22261 aatcctccac tggacaatgt ttcatctgta aaaagcgtca cttgttgaga aacagaattc
    22321 attaataact ccggtggctg attcaaccgc caaactcgac ctccaggacc attgggatcg
    22381 aaaagaaaaa gttcaagaga atgtctgtcc tgattattat ggagaatcct ttctgctata
    22441 cttaatcctc taggtccggc accaatgatc cctattttca tttgcatgcc tccatttcga
    22501 taaatttttt caataaaaca gcatggttct gatgttcatt ttttgcacca taaacaaaag
    22561 taatgactgg atgttctttc ataagttgac tgagtttaaa aatagcttgc ttcttttctt
    22621 cttcgaaaat caattcttgc tggtatttct ttgcaaatga agcaaatcgt tctggtacat
    22681 ggtcaaaagt tttgcgtgct tcattactag gtgcaatttc tttcaaccac agatctaact
    22741 gcgctttttc ttttgaaatt cctcgtggcc agaacctatc cacgagtatt ctatagccat
    22801 cctctttttc agcatcaagg taaacacgtt ttaatttgat catagttgtt tccttctttc
    22861 tgccttttcg cttagcttat atatgtagat aatagcacaa aataaaaaaa atgaacggca
    22921 caactatcca ttgcgccgtt catgtgatct tggtcaaaat ctaatgattg attcggtttt
    22981 taaagctgtc atacaatcat ctgttttata cttttttact tttgattttt ccagaccatc
    23041 cgtcatatcc acccttaagt aaataaatct cttgataacc tgctttacgt aatttatttg
    23101 ctgtacggat gctcaagctt ttcttttgat cgtaaatata gacaggctgg tcttttcgaa
    23161 tagatcctaa tgaatctttc aaaacagtgt aagggatgtt tcttgcacct aaaatatgcc
    23221 cagcatcaaa cacatctttt tcgcgaacat cgattacttg tgcttttctc attccttctc
    23281 gaaattcttc ttctgtcaaa gtcgttgctg aacgcttggc cattactcgt agatagactt
    23341 cccaaaagat gattgcaagc acaatcaata gcaaaatgat attaattacc cataaaacca
    23401 tgtaatgcct ccgttttttt aagcaaattt caaggctaat gaggcagcac caatgacacc
    23461 agcttcatta ccaagctctg ctaatttgat ttttgttgat tctcttactt gcgggaaagt
    23521 aaattcttta aaatatttct ctacacggct acgtaaaaat tctcctgctg ccgatactcc
    23581 tccacctaac acaatacttg acggattcaa tgtattcccc aagttggcgc aagctaaacc
    23641 taagtagaag catacgcgat caacaaccat caatgcaaac ggatcgtctt cttgagccat
    23701 ttcaaagata tctttacttg ttacttcttc accgttgtct aacattgctt tcagttttga
    23761 atctcctgcg tattcttctg ccaagtaacg acctagacgt accacaccag tagcgctaga
    23821 cacagtttct aaacatccgc gtttaccgca agtacattcg aacccttcag gatccacagt
    23881 gatatggccg atctctccgc ctgctccgct cacgccgtga atcaaatttc cgccagcaac
    23941 gataccaccg ccaacacctg ttcctagagt aacgaataca acgtctggat cgttttctcc
    24001 agcacctttc catcgttcac ctaaagcagc aacattcgca tcattatcga tagcgaattt
    24061 gattcctgtt cctgcctcga tcagtttttt cacaaattga acttctgtcc agtttaagtt
    24121 atacgcacca ataaccgttc cagcttcgct gtccacgctt cctggggtac ccatgccgat
    24181 tccgataaag tcttcggctt tcatattata taaattcaaa cgatggttga ttgattcaat
    24241 gatttcagga acgatatgct taccatcatc caaaatattg gtatcgatgc tccatttttg
    24301 ttgaatctct ccttctggtg ttaaaattgc aaattttgct gttgttccac ctaaatcaat
    24361 cccaataatc ttcttgtcca ttacaaattt cctttctttc ggctttcttc aattcggtgc
    24421 tctcttttta atatgcgcca cgctgtcatg tatgtatcat gatcaatcaa acggctatca
    24481 tacagattac gcaattcgat agtcattaat tcaatgtcat agatgcgtgc acccacatag
    24541 ataaagatgc caaatttttt gaacagctgc tgtacatcat ataagctctc catctttaag
    24601 cacttccttc atattataca agtaaaccat atttttttaa acccagtaaa atacatatta
    24661 caaaaagaaa tacaaaaact agtgtagaaa gaatcctttg gtgtatcgaa tagcttgtct
    24721 gttgtttcgg tagacccaaa acattcccta acactaatcc gccaattagc ccaccgacat
    24781 gtccccaaac atctacagag gtatccagaa gaccaaaaac aaatgtcaac acaataaata
    24841 aaagaaaatg tctgactagc tgctggatcg ctgggttatg tttaaaatga aaccctaaga
    24901 taaacaacgc accgaacaaa ccgaatatcg aagtactcgc accaccagac agaacattcg
    24961 cttcgttaaa agcaaagctt gctgtatttc ccattatccc gctgaacata taaatcaaga
    25021 aaaaacgcca atggccatag attgcctcga tttgctgccc cataaaatac agtatcagtg
    25081 agttcattac gaaatgcatc agtccaaaat ggatgaagat tggtgtcact aaacgccacc
    25141 attcattgaa gtgaacaaga taaggaccat acattccggc ataataaggg atctcaagtc
    25201 gcggaaataa ttccatcatg aggaagaccg ctgtctgtat ccctaaaaaa gcataggtcc
    25261 ataacggtcg tcttaaccat gcacgcaatt ttatttgctg ttgataattc atgaaaacct
    25321 cccatttcat tttagtcaat aaacaattgt tggatcttct gatcaaagct ctccggttgc
    25381 cattgttcat tcaattgttc acgaaaaaca aggctacatg tttttttatg aaaattattt
    25441 aaataccggt cgtaataacc accgccaaaa ccgattcgat aaccggctct attgtagata
    25501 atacctggta caatgaggag atcgatcatc tccggcaaat agagcttctc tgattgtggc
    25561 tcaagaacac caaaactcga acgtacaaga acggactgtt catccatttc ataaaacgcc
    25621 atctgacgtt tcggcaaagt tttaggtaca actattttct tttgttcttt ttctgctcgt
    25681 tggataaccg gctgtgtatc cagttcaaat tcattcgata aagtcgtccc tatcactttt
    25741 gctgacttcc agtactcgct ggcgaatagc tgtttataga ttcgctgttc tttttccttt
    25801 ttcttgccac ttttggccag atttttcagc ttttcgattc ctttccggcg taatcttact
    25861 ttcaattggt ccaaaaactc acccttcctt ctctcataaa atggtatagg aaaaaaagaa
    25921 aaatagcaat cgaactttca aataaatgca taaatagaat aaaaagaaga cctagaaatg
    25981 attggccttc cttttttaat aaatttcaac tgttcagcgc gtgtttttgt ctgataaaaa
    26041 gtgcatacag aaaatatggc gcaccaaaaa gggcaatcaa aattcctgta ggtacttccc
    26101 cacttggtag aatcactcta gcaaccatat ctgcactaag cagaaaaatg gaaccgaaca
    26161 aaccgctcag aacaaaggtc cagttatttt ctttacgtac gactagcttc gcagcatgag
    26221 gagctagtag tcctacaaaa gataaactac ctgtcaccgc tacactaccg cttgccaaag
    26281 caacagctaa caacaaaagg attttttgac ttttagccac gttaaggcct aatccttgcg
    26341 cgcgttcttg accaaatgat agcaatccaa tcctctgtgc ataaagtcct cctagcggaa
    26401 ttaagataac cgcccatgga agcaatgccc aaacataagg ccagctcgca ccccagattg
    26461 atcccgctaa ccatgcattc acaaatccat aattttcctt agataattgg atcgtgcata
    26521 gcaatgttaa tgcagaaagg ccggcattaa cggcaatacc ggacaataag attcgattca
    26581 tacccaaaaa gccatttcga tcataggccg caagatagac acagcaagca gctaaaacac
    26641 cacctagaaa ggctacaact agcaatagaa acggcagcga atagtgcgaa aagaaaccta
    26701 aataaagcat cactaaaaat cctgctccgg cattaatgcc caatatacta gaatctgcca
    26761 attcatttct tgttactcct tgcaagatat aaccagaaac gccaagacaa gctcctgcta
    26821 ataaactgat cagtaatcga ggaactcgaa attctctaac aatcagcgcc aaagaagatg
    26881 attctttccc tatgaacgtg gcaaaaagct gatccaggta aataaaaggc gtaccaatca
    26941 ttaaactacc tatagacagg atgacaagta caaaaagcaa gaaatagaac gtccaatata
    27001 tttttttcac cgttgttccc tccttacttg tatcagtaaa aacggaacgc caatcaatgc
    27061 aatgactaat ccaaaaggtg tttcaaaagg gggattgatc atcctcgctg taaaatccgc
    27121 caacataacc aatccaccgc caactaaaaa agagacaggt agaacataac gatagtccgt
    27181 tccaaccatt ccgcgaacaa ctggcggaac caacagccca ataaatgaaa caggtccgac
    27241 taacgaaacg gcaatggctg ctaacactgc aactagacag aaaacaatca accgcgttcg
    27301 tttaggatga cttccaagag ctaatgcatg tgtttcactc aaatgaatca aagatattgc
    27361 ttttcctaat gaaaaagaaa ccaaaacagc aagtatatat aaaggaaaca atactgctaa
    27421 ttgtttccat gtgatattgg cagctccgcc tacaaaccaa aaagcaagat cctgctgcag
    27481 attaaagaat tgggtaagtg cttggctgat agcagtaaaa aaagaactaa tagctacgcc
    27541 agctaaaatc agctgtaaag gattcatgcc cattttcgaa aactgcaaaa aaccaaatac
    27601 gagcaaagtt acagcaaaag aacctgccaa tgacattaga aacacagaaa aatcagaagc
    27661 tttaggccaa aaagcaaaac taagggccat tgccaacgaa gcacccgcgt tgattcctag
    27721 taagcctgaa tctgctaaag gatttcttgt cactccttgc atcaatgcac cgctcaatgc
    27781 aaaacaactc cctacaagaa atgctcccgc cacacgaggc aaacgaatat ttcgtatgat
    27841 ttggtgactt tgttttgatc tatcaaaatc aaacaacgcc tgccaaaaat ctgcttttcc
    27901 cacagtcgct gcgccatttg aaagagcaac aaaaaaaata atcaaaagaa caaaacagac
    27961 caaaagcagg agtctatttt tcttcatagt cctcataatt gatcctcctg ctcttttggc
    28021 aacaaatcat aggttagaaa aactggttta tttgttcttg gaagcgtgac aatcgttgca
    28081 tcaatctcaa atattttttt catacactct tttgtgatca cttcttcagg tgtaccttct
    28141 gtgatcaact taccgttttt cattgctaat aaataatctg aaaaacgtga agcatgatta
    28201 atgtcatgaa tcgacatcaa gatcgttgtt tgtttcattt gattgatttc agtcaataac
    28261 tgtaggatct ctaattggtg tgccggatct aaaaatgttg tcggttcatc caagatcaag
    28321 atatctgttt cctgagcaag cgccatagcg atccagacac gctgggtttg tccaccagac
    28381 agcgttgcga tatcccgcat acgaaatcca gctagacctg tagcatccaa cgcccactcg
    28441 attgcttcat gatcttgcgt gtccatgcca ctgaacagtt tttgataagg gtatcgtccg
    28501 taagatacta cttcttctac tgtcatacct gtagggtgtt cactagtttg tgctaataat
    28561 gtaatttttt ttgcaagttc ttttgaactg tatgtagaga tatcagagtt attgacaatg
    28621 atctttcctg atttcacagg tagtatacgg gtaaaacttt tcaataaagt agattttcca
    28681 cttccatttg gcccaatcaa actagtgatt ttcccttcag gaatcgtaac agacatgttt
    28741 tcaatgattt ttttgttttc gtagccggct gcaatgtcag ctgctcttaa tctcgtcatt
    28801 tcacccatcc tttcgctgaa aatcattctc aattatgacg ctgtttcaac atttcgtcaa
    28861 gcgctacttt catatctata gccaaatgaa gcggttgaca gcgttcgaaa tatcaggaat
    28921 aataaccaat gagaaagatt ctcaattatg ttaggaggaa agaaatgaaa ttaaaaacaa
    28981 aagtattcac attatctagc atattgctgc tggctggagt actgagcgct tgctccccct
    29041 cttcagataa agaaacagct acaactgcca attcgacgac ttcatccaat agtactcata
    29101 cagtcacaga tacacttgga cataaagtag agattccaaa cgcgcccaaa cggatcatcg
    29161 gcagttactt agaagactat ctgattgcac taggggaaaa acctatcgcc cagtggactg
    29221 ttggcagtgg ttccattcaa gattatctgc aagatgattt aaaggatgtc ccaacgattt
    29281 cttatgacct tccttatgag aaagtgttaa gctttgagcc tgatttgctg ttgatttctt
    29341 cttccgctac tgtcgaaggc gggaaatacg aacaatatag caaaatcgcg ccaacttatg
    29401 ttgtaaaaaa cggaacagat gtcacatggg aagaacaact aaaagatgtc ggcaaagctt
    29461 tggcaaaaga aaaagaggca gaaaaaatca tttccgatta tcaaaaatcc atcaagaaaa
    29521 ctcgagaaga actagcagat aaaatcgaga acaagacagc tgcagtgttg tgggtgacca
    29581 acaactcagc ctttatggtc agcaaaaatc ggtcaagtgg aagaatcgta tacgacgatc
    29641 tggaatttgg tgttcctgat ctagtggaag aggtctccaa aaaagccact tctgactggt
    29701 ctgctgtttc ctctgaaaag ctagctgaat tagatgctga ctacatcatc cttgtgaaca
    29761 gtgacaaaga tgctgccatg ttcaatgaac caagctggaa gaatcttaaa gcagtgacag
    29821 acaatcatgt actgcaattc ggacctgaat ctagttggct atataacgga cctattgcca
    29881 gtcaggaaat ggtcaatgat atcaaagaaa aactaaattg attttaacta aaaaaagaat
    29941 cttcacgatt agtcgtgaga tgcttttttt cttattaaca aactagacgc atagatttta
    30001 ttcttctttt gaagcaaccc tattttttac tctttcccat ttttgaaaac gatgggcata
    30061 tagattttct tgatccgtta tcccctcata ctcctctata agggcgaagt ctgaaaaatc
    30121 aatattccca atatacgtat cgccttcaaa cgtgctatcg atcaatgttc gccagatccg
    30181 accagtgtaa ggaagaagtt cttgaaaaat ccgactacca cccgaaacat agatatcttc
    30241 tgagatcgtt tgactatacg acaaaagttc atcgatcgaa tgcattactt ccgcattttc
    30301 tttcgtgcga aaatttggtt gcgttgtcaa cacgatgata tggcgataag gaagtgaaag
    30361 atcccccatt ccttcatagg tctttcttcc catcactaaa attcgatcca ttgtatgttc
    30421 gcgaaagaaa cgcatatcat ttggcaatcg ccatggtaac aaaccatttt tgccgatcag
    30481 accattttta tcttgtgccc acatggaaat aaacatatta tccgctcctt atgattttag
    30541 atgttttgag ataaaaaaag tctcaattct ttgaccagca aggaattgag aactttaaaa
    30601 acattatttt gtttcacggt gcaaagtaac tttgcgttcg cgtgggcaat atttttgttt
    30661 ttccaaacga tcaggattgt tacgtttgtt tttgcttgtt agatagttac gttctttaca
    30721 agaagtacac tctaaagtga tgtttacgcg catgtttttc cctcccattt ttacaagaat
    30781 cctctaagat gactctcaga cttgattatc ttaccatgat tttttctaga ttgctagtgt
    30841 ttttcacgat tttgttaagt aaaactactt gacgataaat acaacggttt tacttctcaa
    30901 acaaaatctt tttctttttg tataaatacc cttacacttg tttatataga ttatgttttt
    30961 taataaaggg ggatactaat atgccatgga acatgaagga ctttcctgca tcaatgaaaa
    31021 acttagataa attgacaaga aaaaaagcaa tcgacatcgc aaatgcatta ttagatgaag
    31081 ggtaccctga taaccgtgcg atcccaattg ccattgatca agcaaaaaaa tggtatgaag
    31141 atgctagtga atcagaaaaa cgaactttcg aaaaagaaaa aaatccaagc aagacggatg
    31201 aacacgatac gaatcctcgt gctggaaaac tgcttgattc agatgtgatc gtagaatatg
    31261 aggaagaaca atggatcgtg aaatctaaag gggcaaaaaa ggcaagcaac cattttgata
    31321 caaaaaaaga agcaatcgaa aaaggaaagc aagtagcaca aaacaaagaa tcaactttgt
    31381 tcatttataa aaaagatggg acaaaagaaa aagaaatcga ttattgaaca tatgtaaagg
    31441 ggttgtgacg aaaatcgtgt cacaaccccc ttatgttcga aacaatggtg ttcctctgcc
    31501 aactccgaac cataagtcat aatatctata aaacgggaag agctgttttt tgaaattgtt
    31561 tcttatcact cagtgttaaa cggtaggaga aaacctcttt tttattgata tttgattttc
    31621 atcaaacagt cacatagtcg attgcttctg cttgcggaat cgtttcatct acaaagtaga
    31681 ctttatacca tgtcaagaaa ctgaagattg tccagatttt tctttgctct tctttacgtc
    31741 cttcgtgatg gtcatctagc aacttcatga ttttttcttg atcaaaaaat tcttttgcaa
    31801 aatctgcttc gaataatgct ttgacttgtt cgtagccgtg ttcttctttc aaccaagctt
    31861 tgattggcac agggaatcct aatttcacac gattcgacca ttcttccgga agatgtttgt
    31921 tcgcagcttg tctgaaaata tcttttgtgt tgttttgatt caataaaaac ttacttggta
    31981 tttgttgagc caatttcatg acttcgatat ccaataaagg tactcggact tccaaagaac
    32041 ttgccataga aagtttatct gcctttaaca agatatcttt tggcatccat tgatgaagat
    32101 cgacatattg catcttattg acttcatctt tgattccttc tgttttctcg taatgaacag
    32161 tcatgatttc atccacagat ggtgctgttt gatattttgg tgttaacact tctgatgctt
    32221 ctgattcttc gaacactcga gcatgaccga taaaatattc ttttgcagga gcaagtgatt
    32281 catataaatg gatccgtcca tggaaatttt tcatttttcc gattttacgt cctaaattat
    32341 agcgtgttcc ttttggtaat tttttcaatc cttgagcaat cacacggatg aattttgaat
    32401 tcgtatggaa tccatacgtt tggtatcccg caaacagttc atccgcccct tctccagatt
    32461 ggactacccg cacactttgt gaagcaagat ttgctaaaaa gtacaaaggt acgcaagaag
    32521 gatttgaatc aggttcatct aagtgatact ggatcaaagg gaatgctttg aacgacatgt
    32581 ctccgtcgat cactgctgct gtattatcta agtccaatag ttcggttagt tttctggctt
    32641 caatcgcttc attataggtt ttatcatcaa acccgattga gaaagaatga tctggtttca
    32701 atacagaggt tacataactg gaatcaaccc cgctggataa gaaagaacca acttctacat
    32761 cactgaccgt atgtgcttca atagaagctt gaacggtttc gtcgattttt tcgatccatt
    32821 cttgtcggga atgcgtttct tttgtttcaa aatctgcatc ccaatattgc tgaatgtcta
    32881 gttttccatc tttatacgta taataatggc cttcaggcaa acgatagaca tctttgaaga
    32941 atgtttcgcc gtttaatgga gaatattgga aagtcatata aggtttcaac gcttctttgt
    33001 tcaattcttt gttaaaatca ggatgaggca gaaaactttt gatttctgaa ccaaacataa
    33061 atgttccgtt catttctgca taataatatg gtttgatccc aaagtggtct cttgcaccga
    33121 ataactcatt cttttcatta tcccaaatag caaaagcaaa cattccgcgg atcttttgca
    33181 ataattctgt tccccattct tcgtaaccat gcaaaaggac ttctgtatct gcatgtgttt
    33241 gaaaaacgtg gccagctgca atcaattctt ctctcaaagg ctggtaatta tagatttcgc
    33301 cattaaaaat aatgatcttt gtccgatctt cattgtaaat aggctgagta ccgccctcta
    33361 agtcaataat acttaatctt ctgaatccta aagcaacatg tttatcgata tattctcctg
    33421 aactattagg tccacggtga acgattcgat ccatcattgt attaataatt gtttttttgt
    33481 tgtccttgtc gttaacaaaa cctacaatac cgcacattta actttcacat cctaatataa
    33541 aattactgtt tatttcattc atatgcatct tatcatagaa taaatagcgg acgaatattt
    33601 tctctcttac aatttcgtca cgtgaaaaca aaagatgaaa gtttctgttt ttacagaaaa
    33661 taaagtccca atatctaatt taccatgttt tcttttttga ggctacctta ttctcttcgt
    33721 gttaaaaaaa tattagtgga tgctatttcc aaaaaaaaca gcaaaaaaag acacgaccta
    33781 aagatcgtgt ctaattatga ctgtatcaga aaatatgata ccaaacggat ctattcatta
    33841 attaattgac atagtttatc tgttgctctt ttatgactgg ataactgacc aattcatgac
    33901 gatccaagtc ttctttattt atatttacag cagtaatttg actgtcttca tacgtacgat
    33961 aataataaat ccctttatca acgttacagc aagaagaata aatcgtatat tcgtattttc
    34021 cttctccagt atcgcatact cctttttgtt gctcgactga gcctagtata tgaaaaaatt
    34081 gactgatact ttcagattct gaatcacctg atgcagcatt cattttagta aatgtggctt
    34141 ttacaaatcg agaaacagaa gagagatctc caggtaagcc caatcctccc atcccacgac
    34201 tataaacatt caagctgatc tggtttgaaa aatgattttc tggtgttcca tttgatagcg
    34261 agcgataatt gttcagatta aataattgat aatcaaatgg cggattattt gtcaacacac
    34321 cgacaggatt atcatataaa tgaagtccat ctttcatact ttcaattact atcgattttt
    34381 ctttatcagc taataaccag tgtaaaggtg acaagggtag tttttcacta aaatcgatat
    34441 tgactagatt gatagtagcc aataattctt tagcttcgtc tatggttgag cattgcccta
    34501 gtatccatgg aataaactca aatggtgcaa cgttatcttt tccttcttgt aattctttat
    34561 aatcagcatt gccaggaaaa ttcagtcctg ccatactcaa ccctttttca tttgtagcct
    34621 cataatagag tggatagttg gataccccag ctgctattcc tatcattgca taatgtttat
    34681 ctaaattttt tacctttcta aaatcgaaac gataatttct tggagtaata gtgactactt
    34741 cattataaga catctcataa tcaaaattcc ttccaaaata atggtctttc gttgcataag
    34801 tgattgcggt acacatcgtt ttccctccta aaacaaatcg tttttacact tttatcttac
    34861 atctagtatt tttttttatc aaaaaaaagg ccaaagaaga ttgatttact agcatttata
    34921 aaaaatagta ctcaggggga atcaaaaaat attgtaggtt aaaagtacaa caataagggg
    34981 ctgtgacaaa agtcttgtca cagcccctta ttccgaacaa acggtgttca aaagcttgca
    35041 tctgcggtaa taagcccaac caaacgaaaa aataggaaaa gctatttttg tttggccgtt
    35101 cttattactt gatgcaggac agcttcttca caacctctaa tcgttgatat aaggaaggta
    35161 caggatttga acctgcgcgc cggtattagc cggttcgccg gatttcgagt ccggtgcatt
    35221 accactctgc caaccttccg taagtactat ttttaaactt ttttcacgaa atgtcaagcc
    35281 tatttctaat agataaataa cagaaatcgc tttttttagc aaaaaagtcc attgacagtt
    35341 tgcagccaac ggacttaccg atcaaattca tactgataga acactatctg attatttcag
    35401 gattatctaa tgctaaaatc gagtcaattg tccgtttgat cagtgcaatg atttgttctt
    35461 ctgttggatg catgatgttt gatgacatca atgacgctaa gccagtcact gctacccatg
    35521 taccagtatg cagagcatcg acatattcat ccgataattg attgtatctt tcatcttctt
    35581 taactatttc acggaaataa ttgtatgaaa aatcatgcat tttctttccg tccccatgtt
    35641 catcaatgta caatgccatg taaagactat gatgcttttt tgcaaagtag atatagttca
    35701 aaccgagatc aatgatttta tcaccagtac gttcaatagg gaaaacatct tcttttagtt
    35761 gttcaaagat agaatccagc aacgtatttt ttaaatcttc catgtttttg aattccagat
    35821 aaatcggctg tgtcgaaatc cccatctttt tagcaatatt tctcgcagta aatcctttaa
    35881 acccttcctt tgcaacaaca tcataagcag cttttagaat ttgatccttg gtatagactt
    35941 ttcttctcat taaaactccc ccacttcttt actgcagtat atgttgtttt attgtaccct
    36001 atttttagca ttttttgcaa acattttact caaaacgtta tattttaaat ttttttcagt
    36061 cttctttaac ttttttttgc aaaattaaaa aaatttggtt aaattcaatg ggttccctta
    36121 catttttagt tttgacctaa atgatagttg tccaaatctt tcatttcaag ttatagttaa
    36181 agaaaatgaa aaagaaggtg tgtaatggaa aacgaaactg acgttattta tattcatcct
    36241 caaaaacgaa tcgtttcaca gaaaagaaag tatttttatc ttggttttac aggtgttttt
    36301 tttcttttta ttggcttgct ttctaatact cctactgata attggagtgg attgctaact
    36361 attcttacct cgccaagcaa tcttttgaca gattattttg ctttaggtgt tttcggaagc
    36421 gcattcataa atgtagggat tttgacctta ctttctgtgc ttttagctta tcggcacaag
    36481 gtgattttaa acggtccctt atttgcttcg attctaacag ttaccggttt ctcttttttt
    36541 ggcaaaaacc tttataattc tatctcaatc attttaggtg tgtatctata cgctgtattt
    36601 gtcaataaac ctttttctca gtacatcatg atcggactgt ttggtagcgt attatcaccg
    36661 gtcgttagtt atatcacttt tggtatgcgt tttcctttac tgataggtat tttattgggg
    36721 aatttagctg gcatagcaat tggtatactt cttccgccct tagctgctca aactttagtt
    36781 ttccatcgtg gattcacact ttataatatt ggattcacct ctgggttgat tgcgatgact
    36841 tttactgctg ttttgaggtt attcagctat tcaatcgtcg aaaatacatt ggttttcaat
    36901 gaatatcact ttcctctcat ttggattatt tttggctttt tttcgctgac agtcggaatt
    36961 ggtttctatt ataattcctt tcggctttcg gggattcgag aaattttcga ttcatctgga
    37021 aaattgacaa ctgatttcat tgctaattct ggtattggtg ctactttgat caacatgggc
    37081 ttagttggtc tgatgctgtc ttcttatgtt ttgctggttg gcggtcagtt aaatggacct
    37141 gtcatcggtg ctattttatc tgcggtgggt ttcagtgctt ttggttgtca tttaaaaaac
    37201 agttttccga tactagtcgg aatatttatt gcttctttgt ttggaacttt tcatgagatc
    37261 accagtactg gcatgcttgt tgcagctgtg tttgggacag gtcttgcgcc cataagcggt
    37321 ttttacggca gtttttacgg tgtaatagct ggtgtgctac atattgctct tgtccataac
    37381 gtcagcactt tgcacggagg actaaatcta tataacagtg gtttttcaac tggtttcgtt
    37441 gcaggtatcc ttgtaccgat actagataac tttaccgctg tcagaaagga aaaaaagaca
    37501 cttggaaaaa gaattatcaa aaagaatcat cgatgagctt cttacttatt tatattccca
    37561 tgaaataact gaaatacgta ttggagtaga atttgctgag gaagggcttt tcatagagat
    37621 acagggtacg gcagaaactc agccagaaga tttagatgta cttgctgatt tgctgaacaa
    37681 tcctcgggac cctagtatgg aaatctacta tcaagaacta ttaggtaatc cccgacatga
    37741 tcgggaagat tatcatctgt taggtagttt gattgatgac gtggaaatca tgtatgccca
    37801 gccgattatc aatattaaga tctttcgacg tgcttctcat tcctattatt aaccctattt
    37861 tctatttata aaagactgaa tcaaaaaagc agctcctgaa agttcgatta aaatctactt
    37921 tcaggagctg ttcaagcttg acattattga ttttccaatc cttcatacac ttcatctgtt
    37981 acaggttcta aaaactctga attaccagca gtgatcgcca aatgttcaaa ccaactgtct
    38041 tttgtcgcac catgccaatg tttaatccct ttgtgcgtta cgacgacatc tccagccatt
    38101 aatcgtcttg ctggctttcc ttcttcctga taccagcctt ctcctccggt cacaagtaag
    38161 atttgatagc cgtctgtatg gacatgccag tgattgcgac aacctggttc aaaagttaca
    38221 ttacctacac caacagaaac ttcgggatca ttcactaaca tattcagata gctttgcccg
    38281 gtaaatactt gagcgtaagc ttcgtttttt tcacctgctg gaaaaatcac gccattgtgt
    38341 atttcttcgt gttttgtcat catttacgcc tctttcttat cttcgctatt ttcttctaaa
    38401 tagatttctt ttgctaaact aaaagctgac caagcttttg gccattctac ataaaaagct
    38461 aaatgggtaa tgatttctgc tatttcttca ctagtaatgc cattttgctt gccaatcttc
    38521 agatgagctg gcaattgttc aaaatttcct cctgaaatta atgcagatat ggtgatgata
    38581 ctacgctgat gagcagataa ttcttctctg gaccatactt gtccaaataa aacatcatca
    38641 tttaattctg caaattttgg tgcaaaatct cctaattgat ctcgtcctgc ggtttgtttt
    38701 tctgccattt ctcttattct cccttcacga ctgttaggtg attcaactat ggttctaagg
    38761 caactttatt ttctattgtt tcttcgcatt tcattttaaa tgtagtcttt cagcctgtcc
    38821 aatgcttagt ttattcagtc gatatgcttt ttaagcatag ttgctaacgc tttttttatt
    38881 ttaaaaaaaa caagttatta aataagtctt tagttggatg tttttttgag atcaatcttt
    38941 tatacttatg atgaacttac aaaattgttt gttttctgtc atatcaaaaa atggcgcagt
    39001 tagttgcttt tctagtaaag tatagttaac aaagaacctc taaggagttg atgaataatg
    39061 aatattgctg cctctcgaaa actgataaat ttcatatcca ttatcggatt aggcctatca
    39121 atcgcgctga cgatttattt tattaattta ggtgttttca aagatttgaa tgcgttaaga
    39181 ggattagtag gtgattccat cattttagga ccgattattt ttgtcttcat ccaaatcctg
    39241 caagtagtta ttccaatcat tcctggaggg atcagtactg cagctggtgt cctgatattt
    39301 ggcccttatg ctggatttat ctataactat attggaatct gcattggttc gattatcatt
    39361 tttctgctag ggcgtcgtta tggaaagcca ttcatacttt ccatgattag cgataagaca
    39421 tataacaaat atattggctg gctggataat caaaaccgtt ttgagaagct gtttgctcta
    39481 gctatttttc ttccagttgc tcctgacgat gcgttatgtt tgatggctgg attgacaaac
    39541 atgtcagtga aacgatatac actaatcatt ttgattgcta aaccactgtc gattttctta
    39601 tatagtatgg ccttgattta tggaggtcaa tatctaagtg gtcttcttgg ataatattta
    39661 ttttgtaaaa ttagtcattg attgacaatg t
//
LOCUS       DACGLW010000016        37818 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.016, whole genome shotgun sequence.
ACCESSION   DACGLW010000016 DACGLW010000000
VERSION     DACGLW010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37818
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.016"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            complement(159..1469)
                     /locus_tag="IXT53_000916"
     CDS             complement(159..1469)
                     /locus_tag="IXT53_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAQ4904762.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1481..2899)
                     /locus_tag="IXT53_000917"
     CDS             complement(1481..2899)
                     /locus_tag="IXT53_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ4904763.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3139..4707)
                     /locus_tag="IXT53_000918"
     CDS             complement(3139..4707)
                     /locus_tag="IXT53_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HAQ4904764.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4711..5121)
                     /locus_tag="IXT53_000919"
     CDS             complement(4711..5121)
                     /locus_tag="IXT53_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HAQ4904765.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5118..6041)
                     /locus_tag="IXT53_000920"
     CDS             complement(5118..6041)
                     /locus_tag="IXT53_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HAQ4904766.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6038..6916)
                     /locus_tag="IXT53_000921"
     CDS             complement(6038..6916)
                     /locus_tag="IXT53_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAQ4904767.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(6903..8240)
                     /locus_tag="IXT53_000922"
     CDS             complement(6903..8240)
                     /locus_tag="IXT53_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HAQ4904768.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8243..9346)
                     /locus_tag="IXT53_000923"
     CDS             complement(8243..9346)
                     /locus_tag="IXT53_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAQ4904769.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9420..10928)
                     /locus_tag="IXT53_000924"
     CDS             complement(9420..10928)
                     /locus_tag="IXT53_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ4904770.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(10894..11586)
                     /locus_tag="IXT53_000925"
     CDS             complement(10894..11586)
                     /locus_tag="IXT53_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ4904771.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11608..12582)
                     /locus_tag="IXT53_000926"
     CDS             complement(11608..12582)
                     /locus_tag="IXT53_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ4904772.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12882..13754
                     /locus_tag="IXT53_000927"
     CDS             12882..13754
                     /locus_tag="IXT53_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ4904773.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14212..16410)
                     /locus_tag="IXT53_000928"
     CDS             complement(14212..16410)
                     /locus_tag="IXT53_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ4904774.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16642..17634
                     /locus_tag="IXT53_000929"
     CDS             16642..17634
                     /locus_tag="IXT53_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ4904775.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17694..19115
                     /locus_tag="IXT53_000930"
     CDS             17694..19115
                     /locus_tag="IXT53_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HAQ4904776.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19108..20607
                     /locus_tag="IXT53_000931"
     CDS             19108..20607
                     /locus_tag="IXT53_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009224330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HAQ4904777.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20646..22040
                     /gene="uxaC"
                     /locus_tag="IXT53_000932"
     CDS             20646..22040
                     /gene="uxaC"
                     /locus_tag="IXT53_000932"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HAQ4904778.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22199..22882
                     /locus_tag="IXT53_000933"
     CDS             22199..22882
                     /locus_tag="IXT53_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HAQ4904779.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            22884..23720
                     /locus_tag="IXT53_000934"
     CDS             22884..23720
                     /locus_tag="IXT53_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ4904780.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            23731..25485
                     /locus_tag="IXT53_000935"
     CDS             23731..25485
                     /locus_tag="IXT53_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4904781.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(25761..26102)
                     /locus_tag="IXT53_000936"
     CDS             complement(25761..26102)
                     /locus_tag="IXT53_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAQ4904782.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(26170..28341)
                     /locus_tag="IXT53_000937"
     CDS             complement(26170..28341)
                     /locus_tag="IXT53_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HAQ4904783.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            28552..29409
                     /locus_tag="IXT53_000938"
     CDS             28552..29409
                     /locus_tag="IXT53_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ4904784.1"
                     /translation="MKITITLPMNHPETTIRDLLEKEWLVPRKVRHFLRIRKNVWINQ
                     QPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDEHLIIVDKPAGMKTH
                     PNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKNPFVLPILGRMLEQK
                     MIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSALTHVTVNKVLSEQT
                     QVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHALELHMIHPFTQEKIV
                     GKALPGLWE"
     gene            complement(29480..30337)
                     /locus_tag="IXT53_000939"
     CDS             complement(29480..30337)
                     /locus_tag="IXT53_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ4904785.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(30322..33024)
                     /locus_tag="IXT53_000940"
     CDS             complement(30322..33024)
                     /locus_tag="IXT53_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAQ4904786.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(33037..34326)
                     /locus_tag="IXT53_000941"
     CDS             complement(33037..34326)
                     /locus_tag="IXT53_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HAQ4904787.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            34476..35522
                     /locus_tag="IXT53_000942"
     CDS             34476..35522
                     /locus_tag="IXT53_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ4904788.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            35524..37677
                     /locus_tag="IXT53_000943"
     CDS             35524..37677
                     /locus_tag="IXT53_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HAQ4904789.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
BASE COUNT        11174 a         7789 c         6725 g        12130 t
ORIGIN      
        1 acgacttagt tatcttatca cgtctaaaaa catgtgtcaa ctcttttttg gaattttcat
       61 aaaatgtttc aatgaagcgc gacaacgttg tttaatatat cattaaaaaa aataaaggtc
      121 aagctaaaat attatttaag cttgacctta ttataatttc aacgattgtc aaaagtattg
      181 tttgcagctg tgttgccagt ttctatagat aaatcacagc ttttgaaaca taagttttgt
      241 actgcttcga ttcgcccgaa ttcttttgca gttatttggc aatgagaaaa gcagacatct
      301 tctataggct tttcaggaaa tgctactaaa tcaaaagctc gggaaggaag tgagtaacct
      361 ggagaaaggc atgcctgtac gtttttgatg tgtatgtctt tgactttcgt catttgcttt
      421 gattcaggta tttcttcagc cactgcttgc caactttctg gcatttgagc aaggtcattg
      481 ctattttttc tattgtattc attatgccag tccattatcc aagaaaaagg aaattgtaca
      541 tcaatcatat tcagattttc taccctgata ttttcgatca ctccccctct ttcttttgaa
      601 gatttcattc gaataccaca gtctgactga aaaaaatcga tatcgtgaat atatacgtca
      661 ctaactccag cactaacttc gctaccaata gttacaccgt atcctgaacg gattttacat
      721 ctagcaacct cgattcttga tgattttcga ttgactcttg caccattgcc atctctgcca
      781 gacttaatag caatacaatc atcaccacag gccaaatcac attcatagac acgcacattt
      841 gttgaagaat caatatcaat cccatctgta cttggtccat cattgttgcg gatgattact
      901 ttttctacta atacatcgtt agaataagtg atttgcaagt tccaaaaacc tgatttctga
      961 aaaatcaaat cacgaacaac aacatgttct gcatgataca gtaaacaagc gcgtggacgt
     1021 ttaatagcat aatccgcaat ccaacgaagt cccttttgat cgtattcagc tcttgtcccg
     1081 cttttttgat cttttcccca atacagctcc caccaatggt ctccttgacc gtcgattatt
     1141 ccttttcctt ctataagaat atctttcgcc gagagaacat tcaaaatcgc tgctggccat
     1201 ttcatttcta cgccggctac tcgtgtatcg atttctggaa atgcttcgat atctgttctc
     1261 cccttgagga ctgcaccttc ttcaaagata agatgactct tatcttttaa aaaaagggct
     1321 ccgactaaat aaatgccttt tggaataaat acaggtaagt gttctttcgc accttgatcg
     1381 attgcttttt gcagtattat tgtatcatct tgttgagtgt cgtttgtttt aaagttcaaa
     1441 gcattgataa tccctttttt tgtccgcaat acgtttctcc ctatctttca ttgatttttt
     1501 tcaattcttc accaataaat acagtttttg aacgattcag tgggtaacct actagcaaaa
     1561 tgattgcgga tacaaataag cagaatgctg gtaacaagtt tgccatcgta taaatccttt
     1621 cttgtactaa aactgattgg atggctccac ctgttgtaga cgattggtaa ccaatgagtg
     1681 ccagcataaa tccgcctacc gctcctgcac atgcttgtcc tacttttctc gcaaaagaat
     1741 ttactccata tactgttcca tcttctctta agcctgtctt atattgatga taatcaatca
     1801 cgtcagtaat gaatgcccag accatcaaat tgaataaacc agatcctaaa gtagcgataa
     1861 ataaaagaat cagatatacc catgcatttg ttatatgaag gaaatacatc agcaagtaca
     1921 tacatgaaga agcaagtaac gcaaatacgc ttgcttcctt tttaccaaat aattttgtca
     1981 gctttgtagc aaatggtgct aaaactacta ctgttccgta agtaaataaa agagcaacag
     2041 acattgcttg tttattatgg aagtaatcat tgaataaata agtcatattc gtcccagcta
     2101 aaatctgatt gatcacaatg aaaatgtcaa caatgactaa aataatcagt gctcgattag
     2161 ctaacaaacc ttttaccaac tgagaagcag atactttttc gtttttgaca actcttactc
     2221 tttcagtagt caatttacaa gttaacatat atgcgacaaa tgctattgca gcacatccga
     2281 ttgaaatcca aaagaaatga ttccctgaaa cgacttgttg tccactcttg ttatgggtat
     2341 acattaaaat cggaatgaag aatcctgtag ttgatccgcc aatcgcagaa ccaatgcttc
     2401 ggaacgtgga caacgaggtg cggtcattag gatttgagct aattgctgct gccattgctc
     2461 catacgggat attgattgtt gagagaaaaa tcccataaac aatgtatgaa acaaaaatat
     2521 aagcaatctt caccgtcatt ggaagatgtt ggacgaatgg caaaaacaaa atgacagtca
     2581 ctaagcaaaa tggatacttc atttttctga tccaaggaat aaatcggcca gtttctgtta
     2641 actctgagac atcacataat cttccaacca tcacatcagc aaacgcatcc aaaattctcg
     2701 aaatcaagaa taacgtacct acgattgccc ctgaaacgcc aagaacattc gtataataaa
     2761 tcatcaagaa actattgact aaacctaaaa taaaacagtt acctagatca ccgaacatgt
     2821 agcctatctt atcggaaatc ccaaattcct ttttttcttt ttttattgta tttgctgtaa
     2881 cagcattttg tgaaagcatt tgaattcctc ctacctcttc tagtaaacgt tttcaaaatt
     2941 ataactcttt ttttcttgtt tttatattca caatgttggc tttttattgc attatcttga
     3001 cagataaaaa acaaaaaata atctaaaaac attgatgcat aagattgata acttttttta
     3061 cataacaata tatacaaaca aaaaaattca ctaactagtg aaaataagtt agtgaacctc
     3121 atttattaaa gaattaaatt acagatgatc ttttccatca tacttgaaaa actcaaaatc
     3181 agcataactt cctctttttt tatttagatc atgcactgct atcccgataa aatttccagt
     3241 aaaccctcct gagagtatac acatgttttt ttgcggacct actttcttcc attcttcagt
     3301 tgatacgtct ttcaaatgaa actgtgctgt ctctttcttt acagtgactt tcaaaaaagt
     3361 cttcttttct gtgagtggaa tcgtccaatc ttctatttgt gctactccgt tttctgattc
     3421 tatcatacgt aggcattttc ccttgtcctc attccaagta acataacaat aaatataatt
     3481 ggattcattt aagtacagta ccagtccagc catctgatta aaattattag gcatgtattc
     3541 aaatgctgtc tccgcatcaa aagtaaaatc cgtttgccga atagctaaca aatgatgttc
     3601 gaaggtagat tgaagagatt caccggaaat cagacgtaaa aaacctggtc tacattttgt
     3661 atcacaccaa tcagcatttg gcaaaatgcg tctggcattc cagttcttct ttagctctcc
     3721 ttcaaatgta tccataaact gatgatcttt ttcttgttgt acctcttcat ttgtctcaat
     3781 cattatttct tccatcggag ttgtctggct atctttaagt cttaaccaat cgtcggacgt
     3841 ccaatacact tcttgaatag cggtttctct tcccaaaata gccgcaccgt ttaatggtct
     3901 ggtacaaaga tatgtcatgt accatcttcc tgacggggtt tgtactaagc tcccatgtcc
     3961 tgtacattgc aatgtagaag caggtttgtc actagctgtc agcataggaa aaccaggatc
     4021 aagctcgtaa ggaccagtaa tattctttgc tcggcaaact gtaactgaat gtcctcttcc
     4081 tgtaccgcct tctgccgtca tcaaataata atacccgtta cgaaaataca aatgtggtgc
     4141 ctccgttttc gctaattctg tacctgcaaa tattttatag atgggaccat caagcttctt
     4201 cttctctgca tcatattctt gtaatacgat accgctggat ttattcggtg tgtccagacg
     4261 ataatcccat agttcattga caagccattt ttttcccgtt tcaagatcat ggaacaaaga
     4321 aggatcaaag ccgttactat ttaaataaat tggttcggac caaggacctg tgatctcaga
     4381 tgcagtgatc aaataattgt ggacatcttt aaacggacgg cctgcacttt tgacatctgt
     4441 gtaaacaaga taaaacaatc catcagcata actgatctgt ggcgcccata tacttccatt
     4501 tgtaggattt ccttgcaaag atacttgagt ggtcaaaata tccgttgtat gtgaccagtt
     4561 gactaaattt ttcgactcat atactcggat tcctggaagc cattcaaaag aggagacaat
     4621 aatgtaatac cgctctttca ctcttaatat attaggatct ggattgaatc ctcttaaaat
     4681 aggatttttc actttgatag tctgcatgat tcaggctcct tttctcattc ttactcgata
     4741 taaactgaat gccccagctg cgaaataaaa aaatgcaaaa ggaaacgcaa taaactgagt
     4801 gccaatcaac aatatgaatc ctgttataaa atacaaacca gtggcagttg ttaagtattt
     4861 tcctttagca gtcaaaaaca atgctactgc taaaaaagca agtgaaataa acaacacaac
     4921 ggcaaacaga ccagtcatgt cctgtaataa tccaaatgta ttttctaaag acaaagttga
     4981 atgatctaca gcaagcgact gataactttt tttaaactct gacgcagaca tattacttaa
     5041 agtaactgct aacccgccaa agaaaatcaa agatagactg ccaccgacca ttcctagcaa
     5101 tagttcccat tttcttttca tttctgaacc ccctgctttt ctaaatgtat ccattcaggt
     5161 cttttgatcg atgtttcata attgttatta cgttcagaga atttgacagt ctgttcattt
     5221 ttcttgtcat cccaattatc ccaaccagtt gggtgaacat gcccacctat cttacacgct
     5281 tcaaataaga catttgcata cggacgccac ggtcttccta aatagacgtt tctagtatca
     5341 ggttctgcag tcaaggtaca gctttcaaaa cgataccctg ttttttgatt tttcggcgta
     5401 gatgctgctg tgatgtatcc aggattttta ctggctaagc tatggatatg acactgatga
     5461 aaaacggcat ctgccccacc aaatatgaaa tccaccgttc cttcaatcca gcaatattca
     5521 taggtctgac gacaatactc aggattggca gaagccggtg tacaaaaagg agtaccatct
     5581 ttttgcaaat caggcaacgg acccgtgcat aaggtatcct gataaccaga tagtcgacag
     5641 ttttttaaag atacgcgatc tcctgaattg aataaagcaa cagcctgtcc taccttttct
     5701 cctggacctg ctgtattttc gatgtgaaga ttttctaata cgacattcga tccttctaag
     5761 aaaaaagtag cacttcggaa agtccctaac tggctaccgt caagatactc ctgttttgca
     5821 aaacgattac cggagataac tactgttcct gctcccacaa gtttaaaatt ggataatctg
     5881 ctttctatat attcatcata tcttccttct tttactatca agacctttgg atggctatcc
     5941 ggcaggcctg ccaaataatc gattccttct tggatagttt caaaatccgt cggggtatct
     6001 atagcattct ttccaattat cagtttgtct ttcatcctca taaatgctcc cttatgaatt
     6061 gtttccaaat atttctcatt tcttgtgttt ccatatgacc gcctgttaaa gaaaaagaag
     6121 aaaagttttc tggactaccc attgattgat actttttgtc cagccctttt ctcagcttca
     6181 gtacgccttc gtccaagcac atcgtatcgt tttttccaac taagctaaga cgagggcgcg
     6241 gagcgatttt ttcttggatt gctaacgttg tataagctgt caatagattt ggcacatagt
     6301 aataaaaccc atgatgatct aggcggcgat gtagcaaaag tatttcgatg tccacctgtg
     6361 ccgctagatc aatacaaact ttgacacgag aatccaatgc tgtcacccac cagcttaata
     6421 agcctcccat agaaagacca atcgttgcga gccgttgagt atctgtgtct tcccttgtct
     6481 ccaaataatc gatcaagctg atcacatcat aaatacgcat tccccataac gtttttcctg
     6541 ttaataaaaa ttgtttgaac aactcgcttt cagaaatacc tccgcgttct tcaaatcccc
     6601 aagcatcaat cgaccaaaca gcatatccca ttcctgtcag tacttctgca aaagatggag
     6661 aagctaagta aggagcacca tggatcaatt cacttttccc gactgaaaaa tctccgccgt
     6721 gagaatgcag gtaaaggacc ggcggaaacg gtccttcttt ttctggataa gcatagaggg
     6781 ctggtactgt ttccaattca tttaagtaca attgatagta ttcaagcata aaaccgtttt
     6841 gtttttctcg gtataatcgt tttccactag gggtatgtct gctagatgga gcacctaaaa
     6901 aatcattcaa ctgcataagc aaagttttta cgaacaacca gttcatccat attttctgat
     6961 tgatttgatt ggaatttctc aatactgatt tctttagtat tttctaatag aaatgctggt
     7021 ccctcgtgat tttcaatcga catctggtta aaggaaacct tacccgcaaa gccaatataa
     7081 aatccttgtc tgctcatttc ctctattcct gtcatcatag caggaacacc gggaactgca
     7141 tcttgggcca tcgagacatc gatattgtca aatgtgattt cttgtatgaa ttgttccgct
     7201 aatccataaa taaaacctgc agaagcatgg acattccttg ccgtgatatt cgaaaaatgg
     7261 atccttctga atgctggtgt ccgttcatct atcggataag cctttttttc ccatacatac
     7321 ggttctttcc ctcgcggacc acagaaataa taaagattca gtataaatgg acacatcaca
     7381 ttatccatga ctatattgga tactcgaata tcttcaacga tcccgcctcg acctcgtcta
     7441 gatttcaatc gtattcctcg atcagtttct tggaagatac aatttgaaat ggtaatatta
     7501 cgaatactac cactcatttc acttcctaat acgacaccgc catgtccatg gaccatcgtg
     7561 caatttgtga tcgtgatatt ttcgcaagcg atcctttcgt acgtatcttc agtccctgcc
     7621 ttgattgcaa tacagtcatc tcccacgtca atatggcaat tactaatacg cacatttttg
     7681 catgattctg gatcaattcc gtctgtattt ggtgaatccg ctgggttcaa gatcgtcaag
     7741 ttatcaaacg ttgcattact gcaaagaatc gggttgatcg tccaacttgg tgactggatc
     7801 aatttaatat ctttcacagt aattcgatga cagttatgga aactcatcaa tttaggacga
     7861 ggataagcga gattatctgg ttcatttcta aaagtatgcc accatttttt tccatttcca
     7921 tctaatgtac cgaaaccagt gacagagatg ttctctacat tttgagcata gatacaggaa
     7981 gcataaactt ttcgatggac tccttcccaa cgtgaatgaa caacaggata atcttttgga
     8041 tcgtcagaga atttcagaac cgctcctgcg cttaaatgaa gttcgacatt gctttttaaa
     8101 aataaagctc ctgttagaaa ttcacctgct ggaacaacta cggtcccgcc tccgtcactt
     8161 gctgcagcat caatggcttg ttgtattgct tcagtattca attcatcaat tgatgcgcca
     8221 aatgtcaaaa tatcatacat atttataatg cctccacttc aatcaatgct tgcaagaatg
     8281 caccaacgcc tttttgatca ttacaaataa ttggttcact gatgtagtaa gcatatgaac
     8341 catctcgctg atctgctccg cctaaccctg ctacttgaca gtttttattc aagttcagcc
     8401 agccttcttt tgtttccaga acaaattcat caagtaatcc ttgatgtgat ttcttcactt
     8461 gtggaagcca ttcttctttg ttcagcagtc ccaaacgtat tcctttagcc attgcgtaaa
     8521 caaacatact actgccggaa gcttccagat aatttccttt ccgttttgtt tctgttgtta
     8581 cttgatacca tgtcccgctg ttttcttctt gatatggaag taatgcagcc agtgtctttt
     8641 ctagaatttc tttcagcaaa gaacaatctg tttcttcatg aagtaattca taggtatctg
     8701 ctgcagccat caaataccag ccaatcgatc ggctccaaaa atgatgggat agacctgttt
     8761 ttggatcagc ccatggttgg accttctttt catcccatgc atgaatcagc aaatttgttt
     8821 ttggatcgaa tagatgttta taactgattt caaactgtct caaaacatct tggtaatctt
     8881 tttcttcact aaaattctgg ataaattcag cataaaaagg agaacccata tataagccat
     8941 ccaaccaaat ttgataagga taaatttctt tatgccaaaa agctccttcg gatgtgcgtg
     9001 gatggtttgc caactgctgg gaaaggagtt ctgctgcttt tttatatttt tcttcgcctg
     9061 tttctttgta caataaaaag aaaagctttc ctgtatttac atggtcgata ttgtattctt
     9121 caacgctgta gccacggatc gttccatctt cttgcacgaa atagtcgata ttgtctttta
     9181 tgaaatcaaa ataaattttt tcttgggttc gttcccaaag taattggaac cctttaagta
     9241 cgacgccata gtcataagac cattttcctt tataccactt ttcttcatat aagcgaggtg
     9301 ttctttccat gatacttttt gctaatttct ttgaccagtt ttccattatt tgctctcctt
     9361 taaaaattaa aataggattt gggacaaata ccaagaagta tttgtcccaa tcgtctatat
     9421 tactgtgccg ctgctttgta tgcttcctcg tactcttgtg tgattcgatc gcctccttgt
     9481 gctttccagt cttctacagc ttttttgaat ccatcttcgt caagctgtcc catgatgtat
     9541 tgcaccgtag catcaatgac gattttttca atttctgatc ctacttcatt gttcgtcggt
     9601 gaatccaatg gcacagaagg atccattaca gcaaattctt cattttcttt gatcaattga
     9661 ttagagaatt ctttttcagg gtctgtatct ttgaaagaga acgtcacttc actaggacga
     9721 gagcttgata acggttgcac ctcttgttgc caaagatcca tatcgataaa ttcgatagaa
     9781 ccatcatcat tcaattcata atgcgttcct tcgataccgc cagtcatcaa tttatacaca
     9841 tcttcttcta aaagatcatc aacaaattga agtaatttct tcaattgtgc ttcgtctttt
     9901 acttgagatt tgggaaatgc tagcaagccg ccaataccat ttccttctga ccaaacatga
     9961 taaccgccgc cttcaccatc tgagatcttg tttacaggca ctaattccat atcatcttga
    10021 ataccttgac ccatcgtctt cagattcttg atatccacca ttccagtgta gattccagtt
    10081 cttccttgtg caaattgttc ttgctgatca gtcttttggg taactgcaaa gtcttgtgcc
    10141 aaatagccat tctcaaatag atctcttgaa aaattcaaag ctttcatata ctcttctgta
    10201 tcaaattctg gagtgaattt gccattatcg tctacagccc agccatttgg tgctccaaaa
    10261 taagaagtga aaagtttaaa gctggtataa cgaatatcac taggcgcacg atcgccaaaa
    10321 ccaactgtgt cgtccacacc atttccatca ggatcgtttt ctgtaaattg tttagctact
    10381 tcataaagct catccatggt agttggaaca tctagtccta gattatccaa ccagtctttt
    10441 cgaatgacta agccagcacg agcgtagtct ttttgtatcg gtacgccgta caaggcaccg
    10501 tcgatactcg cagcttcgat tctatcttca gaaatttgac ttaaattttt atagtctttt
    10561 aaatattctg atacatccca gaatacgcct gattttaacg cattgcggac agaagagttt
    10621 ttcaacatcg caatttgaag tgtaacgata tctcctagtt catcagaagc caatgcagtt
    10681 gttaatcttt cttcttttga agcatcagga atccagttga atgtcagttc cgtatcggtt
    10741 ttttcttcga tcaagttcac aatcgtgtct gtaggcggtg aagcatgatg caaaatattt
    10801 aaccaagtga ttttagcttt tccatcttct gtctctgaag aactgttact tgaactgctg
    10861 ccgcatgcgg caagtgctag aattcctgca aaactaagac ctaattttac gagcgttgct
    10921 tttttcataa attttctcct tcactgtttt aataatattt ttgttttctt ctgttttttc
    10981 tgtatatgag ttcttccatt ccaaatggca aaatatagaa tttgctgtcc acgtgactgc
    11041 ggaaaagaag agactgccac cgataaaggt taaaacacct ggcattgttt ggatgatcat
    11101 cagataaatc cctaccatac ttaatatcaa cactaatgag taatgaagat gtgagaatcc
    11161 gaataaaaat gccattttca ttttgaaaaa atatcctttc caatcataat gagccatgat
    11221 tgggaaaata tagagtgctg agagcacata tatgaacgca gcaatataca aagcgatcct
    11281 aagataccaa ttgtgtatga aagaaagatc cacaagtaaa acaatcccga tggcgccata
    11341 tatccagctc atcagcaagg tttcacgata gttttcttta aaatatgtga agtacgtttt
    11401 cgtcaaagga aacgtttctt ttgagcgcag ccattttcta atcactgcaa ccatcgcata
    11461 cgatgcagga ccgaaagtaa aaactcccaa acctgcgatg actaaaccgc cccacagaaa
    11521 attcaaataa accaactgta aaatacgatt tagccttccg tttattttat ctaatgactg
    11581 cagcatatct ttccctctct ttctatctta gtaaacgccg tcttctccta gctttttggc
    11641 aatcttatta gctaaaatta ccataaacaa tcctacgacc cctttaaaca atcccattgc
    11701 tgtactgaaa cttaactgtc cattcttcaa tccagctgta taaatatagg tgtcgaaaat
    11761 ttctgcaacg cttcgattca atgaattcaa taacaagtac atatgctcga accctaagtc
    11821 taatgtctga ccgattttta gaatcagcaa ggtaatgatt actggacgaa ttgccggcaa
    11881 tgtaacatgc catgttttgc gtaagcgtcc tgctccatcc atttctgcag cttcatacaa
    11941 ctgtgtatcg actgcagtta tcgctgaaag gtagatgatc gtagaccagc ccaactcttt
    12001 ccagatcact tgtccgatat atactgtgcg tgtccattca ggggatgtta gaaaactgat
    12061 tttttcaaat cccattgttg caaggaattc attcaatacc cctccgtcaa cattcaaaaa
    12121 tacataagta atcgaaacaa tgattaccca agacatgaag tgagggatgt aaatgatcgt
    12181 ttgaatcccg tttttcaatc gtttgttcgt tacttcattt aatagtaaag acaaaatgat
    12241 tggcatcgga aaaaacacga agatatttaa tccgaacaag atcaacgtat ttcttaataa
    12301 tgtaaagaat gtcggttctg taaacaaacg aatgaaatgt ttgaaaccga cccaaggact
    12361 tcccattatt cctaaaaatg gttgataatc ttgaaaagca ataacgagtc cccccatagg
    12421 aagataccta aaaatcaaga aataaaggac tcccggcaga atcatcacat ataaaaattt
    12481 attcgttttg attcctgcaa ttattttttt tcgtcttctg agcttttctt cttttttcga
    12541 tagcgtcagt tgaaatggtt cttgtttctt tacactttcc ataagcccct ccttagtttt
    12601 gtgaatgttt gctgcttatg acttcatgat agataactat ctgaattacg tatataatca
    12661 tttttaccgt aagtaaaaac gcttacatag caatgatttt tgtgctatac aaaacgataa
    12721 agtgtatttt ttcacaaata aactacgcat agcaaacctt ctattttttt ctatctgaca
    12781 cttagcatag gaaatgatta tatacgtaat ctttcaccga tgataaattg aacatataga
    12841 aacagaaaca tttaatttat tttgaggagg agcctatcta tatgaaaaag aaagtctcta
    12901 taggagaaag aagttttaca cttttcaaca atacgtttct aattttgcta gctttgatct
    12961 gtatcgttcc ttttttaaat atcatcgcta catcttttgc ttctacacaa gaagtcgtcg
    13021 cgaaaaagtt tatccttttt cctactacct tttctttaga cgcgtatcgc tacattcttt
    13081 ctacgccgac catttttaga gcacttgctg tttcgattgg tgttacaggt gtcggaacga
    13141 tcgtcagtat gtgtgcaact tcactgatgg cctacggtct ttccagaaag tatttgtttg
    13201 gtcgaggttt cgtcaacttt cttgttgtat tttctatgtt gttcagcggg gggatgattc
    13261 caacgttctt agttgttcgt tctctagggc tggtcaattc ttactgggcc atgatcctgc
    13321 ctgtggctgt taatgcaatg aatatgatca ttatgagaaa ctttttccaa gccttgccgg
    13381 atagtttgga agaatcagca aaaatggatg gttgcacaga ttttggtgta tttttcaaaa
    13441 ttatgctgcc attagcgtta ccgtctattg ctactatctc gcttttttat gctgttactt
    13501 actggaacac ttatatgact gctattttgt atatcaacga ttcatctaaa tggccgatac
    13561 agattctttt acgccaaatc gtcattgtat caagtgggat gcaagcagaa agtagtgccg
    13621 ttgatgtgat tccaccagca caaacagtta aaatggctgt tatcgttatt gcaacagtgc
    13681 ctatgctgat tgtttatcct tttgttcaga aatattttgt caaaggagca ctcgtaggtt
    13741 ctgtcaaagg gtgattatgt actgaacgca aaatggtagt tgtgtcagaa gtggtcagct
    13801 tcaagaaata agaaggcatt tacgaaaatt gcttcttaaa tttttgtgaa atttcggctt
    13861 atttttcgaa gaatctactc ctggaacacc gtgtatcaga ggttgtgaaa gagacgttca
    13921 gccttgagcg atgataaaaa ctctcacaaa cattgcgacg agtaacccac aggagcaata
    13981 agaagcgatt ttgaaaaata gcttcccata tttcacaaaa ttatgcctta ttgtcgatag
    14041 cctgtctctt gaacgccatt tattcgtaaa aaagaactgt gacaagactt ttgtcacagt
    14101 tctttttgcg tgcttagcct tgaaatttaa ctatccataa atacaaatac tagggttatt
    14161 ctaacttctt ttattgcttg aatcagtcca caagaaaaac agcctcttga ttcacaaata
    14221 tttttttcga tattctcccg gcgtaatcga ttccttcttt ttaaagaaac gaataaaatt
    14281 ttgcggattt cgatattgca atctttctgc aatctctttt acggagaggt tagtctcttt
    14341 caaccatttt tttgctacct ctaaacgatg attttgaaca aaatcaccaa atttttctcc
    14401 tgtttccttt ttaaatacat tacttaaata gttagggtta taatgcaacc tatcagcgat
    14461 cacatctaaa gacagatcac gatcatattc tgtgtagacg atatgcataa tattctccga
    14521 caaattttta aattcatgat cagtgattgc ttgagagcat tctgtgattg gcagaactaa
    14581 ttgttctaaa acaagttttt ctagttcttt gggatgataa ttgttcagtg cttttacata
    14641 aatttgtttc atgttatcaa agttttttga atctactccc aataactgtc ctaattgaac
    14701 gagttcattg atcaagcgga ccgaaactac ttcccgactc aaaggatttt tattttgtgc
    14761 aaacaatgca tcaatcaatt catgtactaa attcttcact tcctgatttc cagaacgaat
    14821 ggcttcaaac aatcgatttt gttcttctat cggatatcta ataagtgttt tttcatgagc
    14881 agcaggaacg atttcctgat aaaaaataat tgaacttgga ccagtattta cacgataata
    14941 cagggcttct tttgcccgat caaccgattg cttgctttca ttcagcgttt cgaaccggtt
    15001 actgatgcct atactgatag ttaaattcaa gtattctttt acacgttttt gaatgagtcg
    15061 gcaatactcc atgacttttt tgcttgcttc tgcatcatct ttatccattc gataaatagt
    15121 ggcttgcatt tcttcattca aaacgatcgg cagcattcga ttttcctttg gcacaatttc
    15181 cgtgatcatg ttgttgatcg ctaaaagtag caaatcacgc tccccaccgt gactatcttc
    15241 taacagatcg atttgaacca aagctgtata atagcagtca ttttgagaac tataaccaaa
    15301 ttgctgcaac cgctgatcta gttctcgcct ttctactcga tttctaaata aactaagtac
    15361 aaatagcgtt tccagctgtg gcttttgggt aactaagtgt gcacttaacg cttctttttc
    15421 tcctatgatc cgactgactg attgagcgac caaatctagt tctttcccac tgaatccttt
    15481 tgatttaaga ttcagacgtt cctgtatttg agaaatcgga cgggcaaatc tgtccgaaaa
    15541 taagtaggaa agaagaccaa tgagtcctac cagtgttaaa gacactgcaa ctagcccgat
    15601 tctcagattg cggatcattg cgccaattgc actagagtca acttctaatc tgtaaatcca
    15661 attattataa tctgatttca gtactatata cgttttatct ttttctctta tcactttcag
    15721 ctgattgtaa tctagatcca cgtccgttaa tacagttggt tgctctttat tttttactgt
    15781 ctgagtagta aaaaggttgc caacttcgtt ttcaatagaa agtatgtctt ctccactttc
    15841 accaacaact tgttctattg tatgtttctc tatcgtagca attcccaatg catatttttc
    15901 tttttcaaac attggcaaag aaatcaccat ttcgattcct ttgtttttct tttgccaaaa
    15961 taaattattt ttgttattta tgtacttctg ttgatacttt tcaatttcct cttcggtcag
    16021 ttgactcaac gatccattga ttactcccca tttaccagcc aaactgacta aatcataatt
    16081 attatcatcc ataacaatcg tttctataaa atttagttct ttcctgattt cagaataagt
    16141 ggagtaatct gtatatgtca gcggatgctt gaatatgtcc gagaaatttt tggtcgaacc
    16201 atacgtagaa aacgaatatt caatcgttct aattttccat tcaacgttag ttttaacttg
    16261 cctgagcaaa ttctttttgt ctgttaaata actcgattcg atcgattcat aggtattctg
    16321 gtaaatgaat aaactaaatc ctatgacaag tacggctcct aataaaaaca tcggtaaaaa
    16381 tattttataa aatactgtac ttttcttcat tttcttcctc tcctcctttt aaaataaaag
    16441 gctttcattt ttctgtaaaa ttgatactca tctaattaaa tgagcattcc ccgctttcaa
    16501 gaatagcata attaaaaata aaaatccaag taatttttaa cgtgtgcaat cttttcatat
    16561 caaagatcta ttgacttatc gtagttatcg taatatgatg aatgcgttaa caaaaagtga
    16621 aaaagtaagg aaaatgatgg aatggtaacg atcaaagatg tcgctaaagc agctggcgtt
    16681 tctcatacga cagtttctcg tgctttgaat aacagcagtt taataaaaaa tgaaacaaaa
    16741 caaaaaatac gacaactagc taaggaaatg aattattcgc caaactatag tgcaaaaggt
    16801 ttagtgaatc aaaaaaaata tctaatcgga ctatttttct caagtttgca taaaggaact
    16861 tcatcaaatt tcctagcaga ttccattgca ggcatccatt cagtattaga cacaaattac
    16921 agtttatctg ttgaaagtat cgatgttgtt gatttaacag aaatcaacct gcaacgctac
    16981 gatggactga tcgttatgag tcaaagtgat gatgatcagc ctttcatcga ctatctcaaa
    17041 gaaaaagaat ttcctttcgt tgttgtcaat cgtcacatca atgatccagc ggttatcaac
    17101 gtcactgccg atgatgcggc cggtgttatg caggcagtag attacggtat tggatgcgga
    17161 catacgaaga tcgcttatat tggcggaaag aaaaattttc gttcgacaaa agaacgggaa
    17221 aaaggggtat tgaaaagtct aaaaaaagcg agcatccctg tcaatccagc ctatttttta
    17281 acaggagatt acagcttaga aagcggcctc caaaacatgg aatacctatt atcactttct
    17341 gacctgccta ctctggtttt ttgcggcaac gacgacatag cgattggtgc attgagagct
    17401 gtacatttag ctggcctccg tgttccccag aacatttctc tgatcgggtt cgatgattct
    17461 ccagtcgtct cctatctcaa tccgccgctt accaccgtac gtaagccttt gaaagaaatg
    17521 tgtcaagaag gaacaaagct tttactagat ttgatcgaag gcaggacgat cgaacaatct
    17581 aaaattgagc tctccacctt gttgatcctt cgtgagtcga tcaattactt gtaaactaca
    17641 agtaatttta tataaaaatg ttaacgtgtg cataacacag aaaggaagtt tatatgaaaa
    17701 aactgacaaa agaattagtg aaaaaaaacg aagcacctgt aaaagttctt cagtttggtg
    17761 aaggcaattt tatgcgtgga tttattgatt ggcagattca gcagctgaat aaccaacaat
    17821 tgtttaaagg taatgttgca atcattcaac ctttagaaca aggtcttgga aatatgatga
    17881 accagcaaga taatttatat acggtcattt tacaaggact cagcaatggc gaaatcgttg
    17941 atacttccga gattatcacc gtagtagaac aaatgatcaa tccttatgat gaatgggatg
    18001 cttatctatc tctagcagaa aatgacgact tggcctatat cgtctccaat acaacagagg
    18061 caggaatcca attcgtggag actgataatt taacagatac tcccccacag agcctcccgg
    18121 gcaaactgac agccttttta taccgacgtt tcaaattaaa caaagctggc tttaccatca
    18181 ttccttgcga actgattgac agaaacggcg aacgtctaaa agaaatcatt ttacgctacg
    18241 cagaaaaatg ggatttagaa cctgcttttg ttgagtggtt agaaaaagaa aatgttttct
    18301 gttgtagttt agttgatcgc atcgtcccag gttatcctcg cgaagatgca ggtgttttca
    18361 atcaacggca taactatgaa gatcaattga tggtcaaagc agaaccattc atgctttggg
    18421 tgatcgaagg gcctaaagaa ttggagaatc aactaccatt caaacaggct ggcttgaatg
    18481 tcatcatcac agatgatatg actccttatc gtgaacgtaa ggtgcatttg cttaatggac
    18541 cgcatacagc gatggtccct cttgctttgt tggcggaatt ggaaactgta gaagaagtaa
    18601 tgaaagatcc tttattctcc gcatatgttg atcagttatt caatctagaa ttgattccta
    18661 tgctttcttt acctaaagat gaattagcca tttatgctga ccaaatcaaa gaacgtttct
    18721 tgaatccttt tgcccatcac aaattagaag cgatttcatt aaatagtgtt tcaaagttca
    18781 gcacccgtct gcttccagtg ttcaagaagt atatcgaaga acaaaatcag gtaccaccgt
    18841 taatcactgt ttcactagct gcgctacttc ttatgtatcg aggcgatcaa gtaaagccac
    18901 atgatgatga aaagacgata agcgaattta ctgatgcttg gtctgataat ggaacagcta
    18961 ttcctcgatt attgcaaaac gctgcgcttt ggggagaaga tttgtctcaa ataccgaatg
    19021 tgacagatac agttcaagaa atagctgcaa agatcgatca aaaaggtgtc cggtcagtat
    19081 tacaagaaat gaaaggaacg atggtccatg cttgaaaccc tgcaaccaac aatgaaatta
    19141 aatcgacgag acagtgtggt cgtcgcatta acccctattc caaaacaaac acaactgacg
    19201 attgatcagc aaacgatcac cactcttgaa gatatcccgc aaggtcataa gattgcattg
    19261 ggtgatttga aagctggaga caacgtgatc aaatatggct atccaatcgg tcatgtgacg
    19321 acagacgtaa ctgccggtca atggctgcac actcataatg taaaaaccaa tctttctggt
    19381 gaattggatt atcgatatga aaaagatgtc catccaagtc attacctttt tgagaaccga
    19441 acatttcaag gttacttgcg aaaaaatgga aaagtaggga ttcgtaatga tttgttttta
    19501 gtacctaccg ttgggtgtgt gaatggcatt gctgaactga tcgtcaaaca gttcaaggaa
    19561 aaacatccag atctgggaca gtttgaccat attacgattt taaagcatcc ttatggctgt
    19621 tcgcagcttg gaaaagatca tcaaaatact cgagaaattt tagcggacgc agtaaatcat
    19681 cccaatgccg gaggagtttt agtatttgga ttaggttgtg agaacaatac agttcctgaa
    19741 ttcaaaaaaa ttttaggtga atatgatgaa gaacgagtga aatttctagt ggctcaagat
    19801 gtttacgatg agatcgaaca aggtgtggct ctattagaag agcttttaac agcggcggaa
    19861 aatgaccagc gcatatcagt cccgctctct aagttgaatg tcggtttgaa atgcggcggt
    19921 tctgatggtc tatcgggaat aactgctaat cctttacttg gtgcattttc tgactacttg
    19981 attgctcaag gaggcagcac gatcttaaca gaagtgccag aaatgtttgg cgcagaacaa
    20041 gtattgatgg cacgagcgga aaatgaagaa gtttttgact caatcgttga cttgatcaat
    20101 gacttcaaac agtatttctt gtcttacggg gaaccagttt acgaaaaccc atctcctggt
    20161 aataaagcag gtgggatcac tacattagaa gataagtctt taggctgcac gcaaaaatca
    20221 ggaagctctc ctgtcgttga tgtgctacaa tatggcgaaa aaatcagaaa accagggctt
    20281 agtttattgc aagctcccgg gaatgatctt gtagctgcct ctgctcttgc ttcatccgat
    20341 tgccagttgg tcttgtttac cacaggaaga gggactccgt ttggctcata tgtcccaacg
    20401 gtcaaggtat ctacgaatac aactcttttt gaacgcaaag gtcattggat ggattttaat
    20461 gcaggcgtct tattagaaca accaatggaa gtaatcttag aatcgtttgt tcaaaaaatc
    20521 atcgctgtag ctagtggaga agaaacgaaa aatgaacaaa atgacgttcg agaaattgcc
    20581 atttttaaaa acggagtaac actttgattt tcataaaaca gtataaaaag aaagaaggaa
    20641 gatttatgtt tttatctgat gattttttat taaaagacga ttgggcgaaa aagctttacc
    20701 attcctacgc aaaaaacatg cccattattg attatcactg tcacttagat ccaaaagaaa
    20761 tttacgaaaa caacaatttc agcaatctga cagaagcttg gttaggaggc gatcattaca
    20821 aatggcgctt gatgcgagct tgcggcgtgc ctgaagaaaa aatcacagga aatgcttctg
    20881 attttgaaaa atttttagct tggtgtcaaa ccgtaccaaa aattatcgga aatccgcttt
    20941 acagttggtc acatcttgaa ttgaagcgtt tcttcggtat tgacttgttg ataaatgaag
    21001 aaaatgcaga gaaaatttgg acacaagcaa atgaaatttt ggcagctcct gattttcgcc
    21061 gtcgggaaat cgcaaaaaaa tcgaatgtag aagtgatttg tacaacagat gatcccgcag
    21121 acgacttgca ctatcatcag ttgcttgctc atgaagaacc agatttgaag gttctacctt
    21181 cattccgtcc agataaagca ctcaatattg aaaaagaagg atttaatgca tgggtcacac
    21241 tactggaaga agcagctgac caaacgatca ccacctatca agaactgata gaagtcttag
    21301 ggcagcggat agaatacttc catcaacatg gatgtcgtgt ttccgaccac gctttagaca
    21361 tcttacgcta taaagaagcg gatgaaacag tattagaaga gatttttcaa aaagcacgag
    21421 ctaacaaact attatcttca gatgaaatcg atgcttatcg tacagaaaca ctgatacatt
    21481 tgacccactt ctatcattct cataattgga cgatgcagct gcacatccat gcttatcgta
    21541 actgtaatac acaaatgttg gaaaaattag ggcccgatac cggctatgat ggaatgaatg
    21601 atctttcttt gactattcct ctgcaaaaat tattgaaccg tgcagaagaa acggatcagt
    21661 tgccaaagac gatcctttat tcattgaatc cgaatgacta tcctgcgatt ttagctttga
    21721 tgggctgttt ccaaaaagaa cagaatggaa aactgcagtt aggttctgga tggtggtaca
    21781 acgatactcg agctggtatg cgcgatcaat tgacacaatt tgctgatggt agtgctttgg
    21841 ggaattttgt cggcatgctg acagattcaa gaagtttcct gtcctacact agacatgaat
    21901 atttccgtcg tgttctttgt gaatttttag gcgaaatggt catgcgagga gaagcacctg
    21961 aagatgaaca acttttagga agtctagttc aagcgattag ttatacaaat gctaaaaatt
    22021 actttggctt ttttgcctaa ttgattaata gtaacagtaa cgaatcaaaa acaggatagt
    22081 gaaataacgg aatccgttta tccacgtttt agagaagaag acggaccaaa tttgctccgt
    22141 cttcttctct tgctgttctg cttcattgcc tataaacctt tatgaaagaa gagataacat
    22201 gcctactatc catattgccg gtgattctac tgcggcagaa aaagatgaaa acaagcgtcc
    22261 cgagacaggt tggggagaaa aaatctctgc ttatttttca cccgagatcc agatcaataa
    22321 ccaagcaaag aatggacgca gcaccaaatc atttatccag gaaggaaggt tagcccgtct
    22381 agcttcacag tttcagccga atgatttctt atttgtacaa tttggacata acgaccagaa
    22441 aatccaagaa gaaagaggta cgcttccctt cggtagctat ttaaaaaatc tgcagatatt
    22501 ttttgaatgc gcaaaaaaag cgaacgtcca aatggtctta ctgacatctg tgacgcgacg
    22561 agattactta gaaaacggaa cattgaatcc agatatactt ggagactatc caaaagcgat
    22621 gcgtgcattt gctgagaaac accatattcc tcttttagat gtgttctcaa gatcacaaga
    22681 attgttccag accttctcaa aagaagagac aaaaaaattc tacctgcatc ttatacctga
    22741 tacttgtaaa aattacccag aaggactaaa agacaatacc cactttagtc cagaaggcgc
    22801 tgataaagtt gcccgaataa ttgttgaatt gataaaagaa tcccgtctgc ctcttgctaa
    22861 ttatttacaa aaaggagttt gagatgtttc cattaaattt aggcatccgt gcccacgatt
    22921 tagatgttcg aaatcgtgaa gaaatcgttc aaaaagtatc acagcttcac ttgtcccata
    22981 tccaatttgc accacaaaaa gcatttcctg aacaacttaa attaactgac atgacacttg
    23041 ggagtgctgc ctattttggt gaatacttcc aaaaaaacaa tatcgaactt tccattttag
    23101 gatgctatat caacctatcc agcaaaaatc aagaagttcg tgcacaagca ttggcaactt
    23161 tcaaacagca aattgctctg tgctctgcct atcaagccaa gatgattgct acggaaaccg
    23221 gtagtgtctc tgtcggttac acaaaagaaa attttactga agaagcttat cagatcgcca
    23281 gaaattctat cattgaatta gtcgctcatg cagaaaattt tggcgtaact gttgctgttg
    23341 aagcagggat caatcatcca atctataact atcagttagc taaacgttta atcgatgaag
    23401 tagcttcacc aaatttaaaa atcattttag attgtgcgaa tttgatgcat aaagaaaatc
    23461 atgctgatca ggaaaaagtg atccgaggtg ccttggacaa tttacatggt tatattacag
    23521 cgcttcatct caaagactat atcatggaaa atggaaagat ccgcatggta cctgttggca
    23581 agggctgttt agatttccgc ccggtactgc attatatcaa atatgagcgt cctctaatgt
    23641 acgccactct tgaagctact cctgagattt atgttcctga agcaatagaa cacttgcagt
    23701 ttatttacca agattgttaa ggagaagatt atggaggaac gaatacagaa aagttatcaa
    23761 aaatatcatg caaatgcttt tcagacattg ctgcatcttt atcagggaaa ttattggcgt
    23821 tttgccatgt caggactctt ttttattatc aaacatcttc catcttggtt aatgccgatc
    23881 gctattgcca atgtaatcaa tgcagcttca tcgaaagatt cttctcagtt gcaggccatt
    23941 tatttcaacg cttttttgat ggcttgtctg attggtcaaa atatattgac gaattatttt
    24001 catgtaaaat accatagcac ttcgatccgt caagtagaag ctggactaag agcgaccctt
    24061 atcaagaaaa tccaagaact atctattcct tatcaaaaag aattgcaatc tggacgaatc
    24121 cagtcaaaga tcatccgtga tgtagaagct atccaaacgt tgtcctctca agtatttgtc
    24181 agcggattaa atatcgctgt caatttattc gtagctttag gtatcacgct ttataaaagt
    24241 cctgttgttt tcatgttttt cttattgact gttcctctcg ccgcattcgt tactctcttt
    24301 ttccgtaaaa ggatcagaca gtccaactca gacttcaggc aagaaatgga agagacttct
    24361 gcaaaagtga tcgaagtgat cgaattagca ccgattgctc gagctcatgg gttagaaaaa
    24421 cacgaaacaa ctcgcttaaa taagcatttt actcaaatct atgaaaaagg attccactta
    24481 gatatcctgc aatctgtttt cggatcagtc agctgggcta gttttcagat atttcagctg
    24541 atttgtttga ttttcactgg gatattagct ttacaagggc gcatccaacc cggcgatgtc
    24601 gtgatgtatc agacgtattt cacgacgatc gtcaattccg tttctggaat gatcacgctg
    24661 attcctacga tttcaaaagg catggaatct atcacttcta ttggcgaact gttgattgct
    24721 ggcgatgtag aaacttatca cggtaagaaa aaaatccaac atttggatgg aaattttcgt
    24781 tttgaggatg tttcttttct ttatccggac tcactcaaac cgattatcga tcatttttct
    24841 ctagaggtga aagctggaga aacaattgct tttgttgggc catctggctc tggaaaatct
    24901 acactcctta acttattgat tggatttatc cagccgactt caggaaaaat ttttctagat
    24961 gatcagcctt tggatgaact ggatatgcga agtgtgcgaa attatttagc cgttgttcct
    25021 caaaccactt tgttgttctc cgcttcgatc aaagagaaca tcacttatgg cttgaaaaac
    25081 gtatcaaaag aacgtttaga tgaagtcatc gaagctgcgc aactttcttc actgattgac
    25141 cagcttcctg atggtctgga taccttagta ggcgaacatg gcaacaagct gtctggtggc
    25201 caaaagcaac gtatctccat tgcacgagcg ttgatacgtc agccaaaaat cattttgcta
    25261 gatgaagcaa cctcagcttt agataaccag tcagagaaaa aaatccagca agccctgaat
    25321 tacctgacag aaacacccac tacttttatt gtggctcatc ggctttcaac gatcaaagag
    25381 gcagataaaa tcgttgtgat cgatgaagga aaaatcgttg aaataggtac ttacgaaaaa
    25441 ctgttagctc aaaaaggcta tttctaccgc ttgaaaaatg aatgacaaaa aataggagct
    25501 atgacaagac ttttgtcgta gctcctattt ctgaataaac ggtgttctcc tgccaactcc
    25561 tcggtcacaa gtcacaatag tcatcaaaca tgagaagctg ttttttgaaa ttgttccttg
    25621 taactcagag ttaaacggta ggctcacgac ctcttggtaa atggtgttca agagacagcc
    25681 tatcggcaat aagaagcaat tttgtcatcg ctcaaggccg aacgtctctt tcacaccctc
    25741 tgttgtttca taaaatagtt ttattcgctg taaaggtcgc gaactggtgt catgatgata
    25801 cggttcaagt cattgatgat tgtactgaag ccttgctctt tttgcatcaa gtcattgatt
    25861 acttcttctg attggatttt cacagccatt tcttgtgctt tcattgcatc ttcgtcgctg
    25921 aactcttgtc cactcatttg tttttcttgt aattcgattt gcatcgcttg gaagtctttg
    25981 aacagtttgt gtgcttcttc gtttgcttgt actttctcgt acgcttcttt caacgctttg
    26041 aattctggta attcacggat ctcacgttcg atttgattcg cgctatcgta gatgtttgac
    26101 atgttatgcc ctcttttctt ttagtagtta ctctctattg taacatgggg ttattcgttt
    26161 tcaaaggggc tactctcctt gatatttccg ccagaggtct ttagccgctt caccaattcc
    26221 ctgaacgcct tctttcacca tatcgccgaa gtttttcagc ccttcttttg cttgttcacc
    26281 atattgattc aagttatctt gccaatttcc agactctcca ttagaatttc ctgtgtttcc
    26341 aacttgatct gcagctacca cttgtcctcc tgtggcatag gcatctgcta cactaaatcc
    26401 tgtttttgaa gtatacggaa ggatgccttc tgccgctgat ttgaagactt tcccgacaac
    26461 atttccactt gtcccttcca aatagtgcag cttgctggat tcttcgtaac ccatccatgt
    26521 cgaaatcact acgtctggcg tgtaaccgat catccattgg tcatttgctt ttgtggcatc
    26581 gaaattagtt tctgtggttc ctgttttccc tgctatcgta taacctgcag gattcgcagc
    26641 agccgctgtt ccatttgaaa atgtaccaag aagcatgctg gtcatttcat cggctgtttc
    26701 ctttgatatg acctgttttg gttttttgtt tgtgttatcg acgatcaccg ctccagtcga
    26761 atcaacgatt ttcgtgataa gatgcggcgt atacatctga ccatcattgg caaatgcact
    26821 gtaagcaccc gccatgatca atggagatac tcctttttcc agtccaccaa gggctaagcc
    26881 ataatactta tcagattttg ctaaaggcag gccgaattct tctgctttat catatccttt
    26941 ttgtaaaccg atctcatgca aaagccatac agtaggcaaa ttcaaacttt gtgcaacagc
    27001 ttgatacatc ggtacttctc cttggtatgt cccgtcaaaa ttatgcgcat cgtaataact
    27061 ttgcggttcg tctttcaaga tactggaagg cttgtatcca gcttccagtg ctggtgtata
    27121 tacgcttaat ggtttgatcg ttgatcctgg tgaacgacgc atctgtgtag caaagctgaa
    27181 tcctctaaag acatgatctc ctcttcgtcc tactagtgct tgtacaccac ctgtattcgg
    27241 atctaaagct actgaagcag attggaccat ggcaccatct gaagcatttg ctgggaataa
    27301 tgcactgttt tgataagtag cttctaattg cttttgataa tcctgatcta aagctgtata
    27361 gattttatag cctttattca tgacatcttc ttcatcaaga ccatactcgt taactgcttc
    27421 atcgatcact gcatcaaaat aataaggata acgataacca gaatctgaat tatcgtatgt
    27481 gtcattaaga tacgcgctga tatctgtctg gctttctgct tgttcttgtt cttttgtgat
    27541 ttttccgttt tcttccatta cacttagtac tgtgtttttc cgatcattcg cattttttgg
    27601 gtgatcgatc ggattataaa taccaggacc tttcagcatt cctgccaaag tagcagcctc
    27661 agacagcgta acattcgttg catctactcc aaaatacttt ctggaagcgt cttggacacc
    27721 ccataccccg ttgccaaaat aggcattgtt aagatacatg gtcaagattt cttctttgct
    27781 gtacttcttt tcgatctcga ttgccaaaaa taattccttc gcttttcgat caaacgtttg
    27841 gtctaatgtc agatacgcat ttttggcaag ctgctgagtg atcgtacttc ctccgccccc
    27901 tgatgttccg ccagacaaga ccattcggac tgctgcacgg gcaatccctt tgatatcaaa
    27961 cccgtgatgc tcgtaaaaat tcctatcttc tgtcgagatt actgcatctt ggatataggg
    28021 agaaatcgcg tttaattcta catatgttcc tttttgggcg tacagtgtgc cagcttcttc
    28081 gtttttctta tcataaatga ctgtcgattg gcttaatcct gattttaacg tttcgacatt
    28141 tgcttgtttt gctaaaataa agaggtagat acttgtcact aagacacaga tcaaaccaat
    28201 cagcaaaatc aatttgttca catgatattt tttccagatg cgctttcgcc atcgatggaa
    28261 ttgtccgaga tatggtctga tccaacgcca aaaactgatc aaagcagctt ttatcttttc
    28321 taaaatgctt tgaaaatcca tttgcttctt ctcctgtcta tttgattcaa tcgtcttatt
    28381 ttaccatagt gaaagagtgt tttcttcctg cttaagttgt agttcaatat gtttttttac
    28441 gttagtgcaa gtagagtaac ataagaatga acagaaaaat gtaagttttt tcttaaatat
    28501 gttttaaaaa tggtacaatt agtctgcgat tgcactgaaa ggagtacact tgtgaaaatc
    28561 acgatcactt tacctatgaa ccatcctgaa acaacaattc gcgacttgct tgaaaaggaa
    28621 tggttagttc ctagaaaggt tcgacacttt ttgcgtatac gtaaaaacgt ttggatcaat
    28681 caacaacctg ctttgttcca tcatgaagta aagggcaatg accagatcac attgttattc
    28741 gaagaaacag attataatta tcaagaagtt caattaggag atgcgaagca catccgtgtt
    28801 ttatatgaag atgagcattt gattatagtg gataagcctg caggaatgaa gactcatcca
    28861 aatgaaccga cagaaaacag taccttactc aatcatttag ctgcttatct cgacactaaa
    28921 cagcaacgac cttacgttgt ccaccgcttg gacaaagaaa caagcggtgc cgttttattt
    28981 gcaaaaaatc catttgtctt gccgatactt ggaagaatgt tggaacaaaa aatgatctat
    29041 cggagatatc aagccatcgt atggggagaa attgccaaag atatgaccat caccaataaa
    29101 attggacgag atcgccatga tcggagaaaa agggtcgtag atgataaaaa tggtcagagc
    29161 gccttgacac atgtcactgt caacaaagta ctttctgaac aaacacaagt ctattgtgtt
    29221 ttggaaaccg gacgtacaca ccagatcaga gttcatttag ctcataaggg acatccgatc
    29281 gttggagacc ctctctacca aaatagaaca gctgaacgac tgatgctcca tgcattagaa
    29341 ctgcacatga tccatccatt tactcaagaa aaaatagtcg gtaaagcttt acctggatta
    29401 tgggaataga aataaaaaag aacagagtct tatcagcaga taatgataag actctgttct
    29461 ttttgtgtaa tttgtctgct tacttttgat gttcgtgttc tgctaacagc aaactaccga
    29521 taatcccact ttcattcggc agtccccaac gaacgatata ttcacttact tgaggtgttt
    29581 ccatataacc acccatcaat tcaacaaatt gctgacggat tttttgaagg agatgatcct
    29641 gattcattac cccaccgcct aaaataatct tttcaggtgc taacgtcaac gtatagttga
    29701 tcagtgcttg agcaatataa taagcctgaa tagcccaaac aggatgatct tgcggcagat
    29761 cttgcccttt gatccctgtg cgtgcctcaa tagacggtcc tgctgctaat ccttctaaac
    29821 aatctttatg ataaggacaa gtaccttcat aatggtcatc tggatggcgt ttcacccaaa
    29881 tatgtcccat ttcaggatgt gcgatccctg aaaaaatttc ttcattgatc acgacacctg
    29941 caccgattcc tgttcccacg gtaaggtaaa tacaactatt ttttccttgt gctgctcctt
    30001 gcttcaattc cccatatgca gcagcattga catccgttgt ccaagcagca ggaatattgt
    30061 agcgttcctt gattgtgcca agaaaatcga attgtgccca cccttttttg ggagttgcca
    30121 aaacgtatcc gtaattatct tttgttttgt cgataccaat cgggccgaat gagccaatcc
    30181 cgattccctt caattcataa cgatcaaaaa attcaaatac ctgatccaat gtttcttctg
    30241 gtgttgtggt agggatactt aatttttcta aaatttctcc accatctgaa accgcacaca
    30301 caaattttgt tccgcctgct tctattccgc catacattcg ttttcctcct cgaatccgat
    30361 tgtccgaatc tcataaggtt ggattattgg gacaaccgct tgcttcgttg gttcttcaag
    30421 cagattcaat atgacagttt ttccattgtc tttagttatt tccaattttt ctaaatgact
    30481 agacatattg aaacctctta ctactacttt atcactaaac ttgctgcgtt tcaaagctgt
    30541 aattgcaaac gtttcacttt ttatcgttaa atattgttgt tttgaaatca atgttccttc
    30601 atgatgtttg atttgttgag tggaaaaagg aatctgtgct gcgtatgcat gcaaataagt
    30661 agagaatttt tcttctggtc catgtagttc aatgctgtaa ttgaaccgat gctcacctag
    30721 acactgtgct tctggtgttg gaaaatatcc ccaatcgcct agttctccca cactgcgaag
    30781 tagcgtcact gcgatttgtc cgtcttctgt tacttcatat tcattcaaac caaagtttcc
    30841 gactgttaca ccgtactctt catcatgaat attgacaaat gcttgctgat gttgcggatt
    30901 cgtggggttt tcccaacttt tagacacatg gtttgggcgc ttcaccactt caaaaatact
    30961 atctgcttcg tgatgttcca catgtaactt tgtaggaaac aagacacgta agcgatgatc
    31021 tttcatctga ttgtcgagag tcgtttcaaa gtcgattttt ttactatctt ttctgattgt
    31081 catgattgtt tttatttgaa ggattcgttt ttctttggat cgttctgctt ttcgataacg
    31141 aaattccaca accatctgct gttctttttc taaacgctcg tctgcagaaa cagggatttc
    31201 catttcctgt agaatactta tttttgctat ttccggtgta tctactaata cttttacttc
    31261 agagttttct ttgttactgg ataggattgg ctgatcacag aaaggttgtt taaagatata
    31321 ctcatttcct atatcgccga tatcttcaaa aatatgcaga ttattcaact ttcgtttgtt
    31381 tttgcgatct tccattgtaa tggtaccatt tttttcaatc gtcaaatgta gagggccgtt
    31441 ttcgatgatt cgtccactct gatgaatcat tgtttccttt tcttctgctt tcgcttcccc
    31501 ttctacaagc gcgaatgttt cccaagaaaa tgctggcatg ttttctaatg gtaaagtcac
    31561 tttgatcatt cttgccatat atggcacacg gaaacggtct ttcggcaaat cataaccaaa
    31621 ttggacagtt tcttcagata aaatagctgg tatagtggcc ccactttttg tttctacatg
    31681 atacttacgt ttaggcaaat tttctaactc ttgatatagt tgttcaggta ttccttcagc
    31741 gaatcttttt ctttctaatt cgatggttat ttctgcttca ccagttttat ccattcctgc
    31801 agtatttaca atgacaaatg gaaagctgtc ttttgggaat ccggtcgtat cgattgctgc
    31861 ggtcagctgt tctaaggaat cttgtgcaag atactttcca acttcatctg ctttttcaaa
    31921 acgaggtatc atttcccgat ggacagaatc gactgaacaa ccgcaaatac tgtcgtgagg
    31981 atgattttgc attaatgtct tccaagcata atctaattga tcatggggat agtttccact
    32041 aacttcataa gccatcgtcg caagtggttc tgtaatattt tctagctgtc gctggacctt
    32101 ggtattccat tgttttaagt aaacccgagc agatgctgta ttggctaaag tataccagcc
    32161 atccgtttct tgacttgtca attctccttc tactgagcct aagtcttctg gcacatcttt
    32221 ttgaactgct tccagatagt cagtaaaatt agaatgaata aactcgtaat ctggaaacag
    32281 ttcatttgcg agatggattg cttttgatat atctttttgt accggttgat gatcaacacc
    32341 attcatcatc aataagtggt tcgtagaggc atattgttct gcatccgcta atttttgctt
    32401 ccaaaaggca agagctgctt ctttttctac tggaatctca ttcccgttgc tataccaatt
    32461 ggcaaatagc agaccaaaga ttgctgtttg atcaggtcct ttccaccaca tctctgagta
    32521 ttgggaagaa tagttttctg cttccaaaac ttgattatca aagccaattg gttttacacc
    32581 acggccaaac gctgctgctg aaataccagc ttgtttcatt aattgaggcg tctgtcccat
    32641 atttccaaat gtatcgggaa aatagcctaa catgacaggg gttccccatt tacggctttc
    32701 ttccatgcca atcagcatat ttctcacgtt cgattccgag ctgataagaa aatcatcttg
    32761 caagatataa aatggtccaa ttctcaattt tccttcattg attgcttgtt ggattgcctg
    32821 tcttttttct ggccttactt gaagatagtc atctaaaata atcgtttgcc catccaaatg
    32881 aaaactgttg aaggacggat caacttcaaa tagctctagt agatcgtcta tcaattcaat
    32941 cagtcgcatg tgatgctgtt cgtaagccat gtaccattca cgatcccagt ggctatgaga
    33001 aatgatatat actttctttt tcatttacga tacctcctac ttttctactc taatatcaaa
    33061 gtaatccatg actaattcgc aaaacatcat atttgcccaa gaaaaccatt ctctcgtata
    33121 attttcagga ttgttcacat cgaatccttc gtgcataagg tgtgttcctg cgtcagttgc
    33181 tacaagtaaa ttcaatatcc gctctttttc agatttgtct gacgttgtca ttccttccat
    33241 agctaatgca atcggccaaa cgtatttttc tggcgtatgg gaggatccaa ttccttttgc
    33301 gtactttcct tcatagaaat atggattttc tttactcaac aatgttcttc ttgtttgcag
    33361 ataatcttcc tcttgttcag aacaatatcc caagtaaggt gcggcgatca gattgggcac
    33421 attactgtca tccatgactg tggcgtttcc cattccatct acttcatatg cataaatcgt
    33481 ttgccctttt tgattaaccg ttcgtccgtg attctgaatc ccctcttgga tctctttctt
    33541 caatttctgt gtttgctgta caatttcttt atcatcgagt atcgtttgaa aaatttcttc
    33601 cacataacct aacacgacca ccgcaaacat attggatggt accagatatc cataacggca
    33661 agtatcatcg ctaggacgga aaccagacca tgtcattcct gtaaaagctg tttttgcacc
    33721 tcgcccgtca ttgatcagcg tatcttcttt tcttgtcgtg tctcgtacaa atgtataagg
    33781 agagtgttca tgatcttgct ctgttttgaa cacgtccaag atttttttga taccttcaat
    33841 aaaagattga ttgaattgat cagtttttcc tgtatttttg taaagaagat aggccaactg
    33901 tattgggtag cataaagaat caatttcata ttttctttcc cagatccaat cgttcatttt
    33961 tgtatgatct gtttggtggc cagcaccgtt agcttcttcg ttgaaggcat tcgcatatgg
    34021 atcgatattg atataaaaaa attgcttttt gactaacccg ctgatcatgt cagctaattc
    34081 ctcatcttct tttgcaatca ctaaataagg acgaacttgt gcagtagagt ctcttaacca
    34141 catcgctgga atgtctcccg tcaataaaaa ggtggttccg tcattttgct ttttgactgt
    34201 tgtcaacaga gtattggcaa atgctgctgt aaaattcttt gcccagtccg catggatatc
    34261 cccacatttt tgtgtgatat cttccataaa tttctgcacc gattttggta tatctttata
    34321 agccactaat aaaaccttct ttctatttga tatatcttta aagattatac ttcatacagc
    34381 caattatgtc tacgctttca aaaaatatct ttttatttac tttttcatcc attatggtat
    34441 tatttgaata tgatatatca ttaagaggtg tttatatgaa tcaaccattg taccagacaa
    34501 tatttaatga tttgcaaacg gctattttaa ctggtgaatt acctgtagac agccaactac
    34561 caactgaaaa agaactttca cgtacctata aagtaagcag aatcacttcc aaacgtgcat
    34621 tgactgaatt agagcaatta ggacttattt atcgcgttcg cgggaaaggc agtttcgtta
    34681 aatccgcttc tccggaacag attcttgctt ctcctacaaa aacacgacgg attctgtttc
    34741 ttcttccata tgtcgctgat ttgtctgttg gtgattttag caagggattg aaccctgtga
    34801 tgcaagataa acagattgat gtcatgatga cgacgcttga ctttctacaa aataaaacag
    34861 caaaagagct aatcaatgaa tttgacggat tgatttatta tgctcttgat acagaacaac
    34921 atcttgactt actttttgag ctttcattga aagaattccc tgtcgtcata ttagataaaa
    34981 aaatctatga gttacctttt cctaccgttc tttccgataa tttccaaggc ggtgcgcttg
    35041 ctgcctctta tttgatcgaa cagggacatt caaaaatcgc ttatttgttt ggtacgcaga
    35101 ctcatccaca atccgttcga cagcgttacc ttggttacct tgaagcattg gacaaagctg
    35161 gtttgacttt ccacacttca ctggatgaca aaagcgcgac attagatgaa ttgctttctt
    35221 atatcactaa gtatgaagta actgcttttg tttgtgaaaa cgatgttgtc gctatccaga
    35281 ccatgaatct cttgagacag catggttatc atatccctga agatttttca attatcggat
    35341 ttgatgatat ccaagcagca gctcttgttg acccgccact gacaaccatc gcacaagatt
    35401 ttgaacagtt aggtaagctt gcgggagatg ctttggtcac ttggttagag acaaaagatc
    35461 gaccatccga tagtaaattt ccagtttcat tcatcaaaag acaatcaaca aaggagaaat
    35521 aaaatgaata tcggtcaaat agatactaga catgcaacag cgaatcagcc aaatttctct
    35581 aatgggaact gccagcctta tactggcgtt ccttttggga tgaattattt tgcaccgcaa
    35641 actacggatc aaaacggcag ctggtggttt catcctgatg accgcgtttt tcaaggctac
    35701 cgtcttaccc atcaaccaag tccttggatg ggtgatttca gtcatatgct gtttacgccg
    35761 atcaatggaa aactacagga gaacaccctt ttccatgctc aaagttctta ccgcccagaa
    35821 gaaagtatat tttgcccgac tcatctgtcg atctgtcaat tacgttatgg catctgttct
    35881 acattgatac ctagcatgta tggcggaatc ttatctattg attacagcaa aaatgacagc
    35941 ggtttacttt tatcctttcc gggacgccat cagctctttg ttatagatcc ttacacagtg
    36001 gaaggaaaaa tcacacattt tgctggttca gaagatcctg atttttcttt ttattttata
    36061 ttgagatttg aatatccttt aacaattgat gagttagctg tttccaacaa aaatagcgac
    36121 tcgatcccgt tgtattttga acagacaaaa aaacaaacta tccgatttgg gacatctttt
    36181 atcagctcgg aacaagcagc ttataacctg gaactagaac aaaaccaaag caaagagact
    36241 taccttgctc acagtcaaga acaatggcag tcctattttg atcgtataca gctcacacac
    36301 catgatcaac aacaattgcg gacgttttat cataatttat atcgggtgtt tttatttcca
    36361 caaacatttt atgaaattga tcctagtggg caaaaaatcc actatgatac gactagtcga
    36421 tctataaaac ctggtgttct ctatacaaat aatgggtttt gggatacgta taaaacggtt
    36481 tatcctcttt attctttgat tgctgtagaa aaatacgaag aaatgctgga aggttttttg
    36541 aacagctata gagaaagtgg ctatttgcca aaatggctgt ctcctgatga acgtggactt
    36601 atgcctggga cattgatcga tgcggtcatt gcagatgctg ctgtaaaaga tatccgacga
    36661 gacttgatgc ctgaatttct aacggcaatg gaaaaaggtg ctacggttca aagcaaacat
    36721 ccaaattatg gacgccaagg aacagaagat tacttgacat atggctatgt tcctaatcac
    36781 taccacgaat cagtcaatca cacattagac tatgcttata gcgatttttg tattagccaa
    36841 gttgccaagg tactagaaga agaggaaatg acgaagaaat atcagcagca atcattgaac
    36901 tatcaaaata tctttgacta tgagtcagga tttatgcgtt caaaagataa agatggcaag
    36961 ttcagagaac cattttcagc taatcgttgg ggacaagatt atgcagaagg aagtgcttgg
    37021 caaagcagct ttgctgttta tcacgatttt tcaggactga tcggaaaata tggtggcagt
    37081 gagagatata aagaaaaact cattgagctg tgcaatcagc ccccaacgtt tgacccagga
    37141 ggatatggtt ttgagatcca cgaaatgagt gagatggccg cgatagaatt tggacaatta
    37201 gctatttcaa accagccaag ttttcactat ccttacttgt tcagttatat aggacaacct
    37261 gaaatggcac agccactgct taaacagttg atgactcaga ccttcgacga ttctccaatg
    37321 ggttaccctg gagatgagga caatggaagt atggccgctt ggtatatctt caatagtctg
    37381 ggattttatc ctgtcacgcc tggaaccggt gaatatgtta tcggtatgcc attaattgac
    37441 aaggctgttt tgcttttgtc aaatggaaag gaattgacga ttgaagcaac gcctaatcgg
    37501 ccgcagcaac aatttatcca taaaatcaaa cggatggaca aagaatatac gaaattatat
    37561 tttacccatc aagatttgct gaaaggcggc acgatctctt accaattagg gatcatccct
    37621 caccctaaga aatatcaagc aaaagatttg cctttttcct tatcaaacta taattaacaa
    37681 gaggttgtga aaaagctgtc ctgcatcaag taataagaac agccaagcaa aaatagatcc
    37741 tcatattttt cgtttggttg gtcttattac cgcagatgca agcttttgaa caccgtttat
    37801 tgagaggttg tgaaatca
//
LOCUS       DACGLW010000017        36567 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.017, whole genome shotgun sequence.
ACCESSION   DACGLW010000017 DACGLW010000000
VERSION     DACGLW010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36567
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.017"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            complement(25..1521)
                     /gene="lysS"
                     /locus_tag="IXT53_000944"
     CDS             complement(25..1521)
                     /gene="lysS"
                     /locus_tag="IXT53_000944"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HAQ4904790.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1632..2636)
                     /gene="dusB"
                     /locus_tag="IXT53_000945"
     CDS             complement(1632..2636)
                     /gene="dusB"
                     /locus_tag="IXT53_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HAQ4904791.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2637..3524)
                     /gene="hslO"
                     /locus_tag="IXT53_000946"
     CDS             complement(2637..3524)
                     /gene="hslO"
                     /locus_tag="IXT53_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HAQ4904792.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3741..5852)
                     /gene="hflB"
                     /locus_tag="IXT53_000947"
     CDS             complement(3741..5852)
                     /gene="hflB"
                     /locus_tag="IXT53_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HAQ4904793.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5942..6487)
                     /gene="hpt"
                     /locus_tag="IXT53_000948"
     CDS             complement(5942..6487)
                     /gene="hpt"
                     /locus_tag="IXT53_000948"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HAQ4904794.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6487..7932)
                     /gene="tilS"
                     /locus_tag="IXT53_000949"
     CDS             complement(6487..7932)
                     /gene="tilS"
                     /locus_tag="IXT53_000949"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HAQ4904795.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8052..8522)
                     /locus_tag="IXT53_000950"
     CDS             complement(8052..8522)
                     /locus_tag="IXT53_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HAQ4904796.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8568..8993)
                     /locus_tag="IXT53_000951"
     CDS             complement(8568..8993)
                     /locus_tag="IXT53_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HAQ4904797.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9060..9329)
                     /locus_tag="IXT53_000952"
     CDS             complement(9060..9329)
                     /locus_tag="IXT53_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HAQ4904798.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9340..10935)
                     /locus_tag="IXT53_000953"
     CDS             complement(9340..10935)
                     /locus_tag="IXT53_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ4904799.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10950..14471)
                     /gene="mfd"
                     /locus_tag="IXT53_000954"
     CDS             complement(10950..14471)
                     /gene="mfd"
                     /locus_tag="IXT53_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HAQ4904800.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14488..15048)
                     /locus_tag="IXT53_000955"
     CDS             complement(14488..15048)
                     /locus_tag="IXT53_000955"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HAQ4904801.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15401..16375
                     /locus_tag="IXT53_000956"
     CDS             15401..16375
                     /locus_tag="IXT53_000956"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAQ4904802.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16545..17945
                     /locus_tag="IXT53_000957"
     CDS             16545..17945
                     /locus_tag="IXT53_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HAQ4904803.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18023..18745)
                     /locus_tag="IXT53_000958"
     CDS             complement(18023..18745)
                     /locus_tag="IXT53_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ4904804.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18824..19330)
                     /locus_tag="IXT53_000959"
     CDS             complement(18824..19330)
                     /locus_tag="IXT53_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAQ4904805.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19453..21252
                     /locus_tag="IXT53_000960"
     CDS             19453..21252
                     /locus_tag="IXT53_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HAQ4904806.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21320..22894)
                     /locus_tag="IXT53_000961"
     CDS             complement(21320..22894)
                     /locus_tag="IXT53_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4904807.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(23149..23658)
                     /gene="mreD"
                     /locus_tag="IXT53_000962"
     CDS             complement(23149..23658)
                     /gene="mreD"
                     /locus_tag="IXT53_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HAQ4904808.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(23662..24516)
                     /gene="mreC"
                     /locus_tag="IXT53_000963"
     CDS             complement(23662..24516)
                     /gene="mreC"
                     /locus_tag="IXT53_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HAQ4904809.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(24671..25309)
                     /locus_tag="IXT53_000964"
     CDS             complement(24671..25309)
                     /locus_tag="IXT53_000964"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HAQ4904810.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25310..25960)
                     /locus_tag="IXT53_000965"
     CDS             complement(25310..25960)
                     /locus_tag="IXT53_000965"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HAQ4904811.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(25973..26872)
                     /gene="rsgA"
                     /locus_tag="IXT53_000966"
     CDS             complement(25973..26872)
                     /gene="rsgA"
                     /locus_tag="IXT53_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HAQ4904812.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27037..29106)
                     /gene="pknB"
                     /locus_tag="IXT53_000967"
     CDS             complement(27037..29106)
                     /gene="pknB"
                     /locus_tag="IXT53_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HAQ4904813.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29103..29843)
                     /locus_tag="IXT53_000968"
     CDS             complement(29103..29843)
                     /locus_tag="IXT53_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HAQ4904814.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(29856..31214)
                     /gene="rsmB"
                     /locus_tag="IXT53_000969"
     CDS             complement(29856..31214)
                     /gene="rsmB"
                     /locus_tag="IXT53_000969"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HAQ4904815.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31214..32152)
                     /locus_tag="IXT53_000970"
     CDS             complement(31214..32152)
                     /locus_tag="IXT53_000970"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HAQ4904816.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32145..32636)
                     /gene="def"
                     /locus_tag="IXT53_000971"
     CDS             complement(32145..32636)
                     /gene="def"
                     /locus_tag="IXT53_000971"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAQ4904817.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(32651..35059)
                     /gene="priA"
                     /locus_tag="IXT53_000972"
     CDS             complement(32651..35059)
                     /gene="priA"
                     /locus_tag="IXT53_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HAQ4904818.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35312..35617)
                     /locus_tag="IXT53_000973"
     CDS             complement(35312..35617)
                     /locus_tag="IXT53_000973"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAQ4904819.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(35622..36236)
                     /gene="gmk"
                     /locus_tag="IXT53_000974"
     CDS             complement(35622..36236)
                     /gene="gmk"
                     /locus_tag="IXT53_000974"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HAQ4904820.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYINKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
BASE COUNT         9885 a         7988 c         6706 g        11988 t
ORIGIN      
        1 attccttaga cagcttttat tcgattatct cattgttggg aatagcaaaa catcccgaat
       61 tgattgtgca tcagttagca acattactaa tctgtcgatc ccgatgccta gaccaccagt
      121 tggcggcata ccgtattcta aggcttccaa gaagtcttcg tctacaccgt gagcttcgtc
      181 atttcctagt tcacgttctt tttcttgcgc ttcgaatctt tctctttgat caattggatc
      241 gttcagttct gtaaaggcat tagcaaattc gcgccccact atgaatagtt cgaatcgatc
      301 tgtgaatcga ggatcttctg gatttttctt cgctaaaggc gaaacttcga ctggatgtcc
      361 gtatacaaac gtcggttgtg tcaatgtttc ttcaacaaat gtttcaaaga attcattgat
      421 aacatgtccg acacccatag cttcggttac ttctacattg tgtttttcgg ctaaagctaa
      481 tgcttcttcc aatgtcattt cttgccagaa gtctacacca gtttgttctt tgattgcatc
      541 taccatatgg atgcgtttaa agtcactttc taagtcgaca gcttgtccat catatgtgat
      601 ttttgctgtt cctagtactt tttcagctgc gttacggata ataccttctg tcagattcat
      661 tacatcattg aaatcagtat atgctgtgta tgcttctaac atcgtaaatt ctggattatg
      721 tgtagtatca attccttcat tacggaaaac gcggccgatt tcataaactt tttccattcc
      781 gccaacaatt agtcgtttta aatgaagttc tagcgcaata cgcaaatata aatcaatgtc
      841 cagtgcatta tgatgcgtga taaatgggcg tgcggcagct ccacctgcct cattgtgcaa
      901 tactggtgtt tctacttcaa tatagccatt gccatctaaa tagcgacgga tttcgctgat
      961 aatttggctt cgtttcataa atcggtcaaa gctttcgcga ttactgatta aatccaagta
     1021 gcgttgtctg tagcgttgtt caatattcgt taagccatgg tatttatctg gcagtggacg
     1081 aagtgctttt gacaaaatcg tgatttctga agctttgata gaaacttcgc ctgtgtctgt
     1141 tttcatgatt tgtccagtta caccaaagaa atctccaagg tctgcatgtt tgaatacttc
     1201 ataggcctca tcacctactt ggtcttttct tacataaatt tgaatttggc cttcacgatc
     1261 ttgtagatgc gcaaatccag ttttcccttt tcctcggcga gtcatcatac gaccagctac
     1321 actagcggtc aatcccattt ctgctagttc ttcttttgtt cttggatcaa atagttcatg
     1381 caattcttct gagttgtgcg tgcgttcaaa tcgttttcca aatggatcga ttccatcttc
     1441 acgcaattgt tccatttttt gacgacgtac aagcatttga tcatttaaat cttctacttg
     1501 atgttgttgt tcctgtgtca caaaagttcc tccattctca tgttaatctc tttcctataa
     1561 tgccaatgaa acacaaaaaa agcaagccga acttggaatt tcggcttgaa aattcacgaa
     1621 aaacttagaa atcacaacgt ttgtttcaat agtacttttt ccgctttttc tacaaattga
     1681 tccagcaggt cattgatttc ttgctgtttt tctgtctgat tgattgctac tttcactttc
     1741 gctgctcgag gaatcccttt taaataataa gccgcatgtg ttctgaattc tctacaagct
     1801 actttttctc ctttgagatc tgctaagcgt tgtaaatgaa gtttagctgt agcaattttt
     1861 tcggctggtg ttggttcagg aatcagttct cctgtttcga gataatgctg tgtgcggtga
     1921 atcatccatg gattgcccag cgctgcacga ccaatcatca caccgtctgc accgacatag
     1981 tcaagcatcc attttgcatc ttctggcgtt ttgacatccc cgttccccat aaatgggatc
     2041 gtcaaatgtt ttttcacttc tttcaatact tcccagtccg cttttccttc atacatctga
     2101 acccgtgtac gtccatgcat agcgatggca cttgctccag ctgattgtgc tgctaatgca
     2161 ttttctacag caaagacgtg ctcttcatcc caaccaatac gcatcttgat tgttactggg
     2221 atatttactg ctgaagaaac agcttggacc atttcgtata ctttctcagg atctaaaagc
     2281 cattttgctc ctgcttctgc tttgattact ttatttactg gacagcccat attgatatct
     2341 attatatcgg cagctgtgtt ttcttcgaca aattgagcag cttcaactag actatcttta
     2401 tttccgccaa atatctgtaa acttaatgga tgttctgttt cttcaatgtg gagcatctct
     2461 aaagtttttt tgtttctgaa atgaatacct tggtcactga tcatttcaca tacgactaag
     2521 cccgcaccaa attctttgac agtgactcga aatgctgaat ttgttattcc agccatcggt
     2581 gcaacaacga cacgattagg gatttccaca ttgccaattt tccatgtgtg ttccacttaa
     2641 atccctccta aggcttcttt cttcaatcca tctaattctt cttcgctata cttgtatttg
     2701 ttattacaga aagaacaaac cgcttctgca ccgtgatctt cgtctatcat tgcttggatt
     2761 tcttctgcac ctaacgcgat aattgcttct gcaaattttt cttttgaaca gtcacatttg
     2821 aattgaacag gcattttttc caaaatgttc aattcttctg taccaagcaa tcctttcaat
     2881 atttcttctg gtgtctgtcc ttcatctaag agggtagaga ttcttggtgt cgtttttaat
     2941 tgttcttcga tcttgctgat tgtttcttca tcggcacctg gcatgatttg gatcatgaat
     3001 cctcccgccg tcttgatgct gtcgtcagta ttgaccaata cactcagtcc aacagctgaa
     3061 gggatttgtt ctgagatagc aagatagtaa gtaaaatctt cagcgatttc tccagaaaca
     3121 atcggcgttt gcccagaaaa aggttctttt aatcctaagt cttttgtgac agtaaacatt
     3181 ccttgtgttc caactgctcc ccgcacatcg attttacctt cactatttag aggaagactg
     3241 agatgcggat ttttgatgta acctttcaca tctccctttc catttgcatc tactacaatt
     3301 cctgttgctg gaccatcgcc ttggattcga acagttagtt tgtcttctcc tttgagcgtc
     3361 gcaccaagta gcagactacc gaccaatgtc cgtcccaaag cagctgagga ggtgtgccaa
     3421 gtatcatgac gtctttgtgc ctccgctacg gtatttgttg cttgaacggc gtatgctcgg
     3481 acgaatccgt cgtgagcaag tgccttaacc aaataatctt tcatttgtta tgtactcctt
     3541 tttccgtttt taaactcata gtgaatcata ctagcaacta ctgctttttt caactttgga
     3601 atgcttcttt taaaaataat tcagaatcat ctagaaaaca ggacatcacc ctatttacaa
     3661 cttaaataaa gaaatctccc gaaaaccggt ttttgttccg gttttcggga gtcatatcag
     3721 atcaattgac ctatattttt ttatttgtat tgatcgtctg aatgattatc ttctgattgt
     3781 tgagaatctg aatcatgttc tgctttattt tcctcattca gttcatgttt tgcttcttca
     3841 aaatcacgtt tttcttccgc ttgtttttca gcgtcttttt cttccaaagc acgttttgct
     3901 tcttcaaaag ttttcgcttc tttttcgctt ggataatcat ttgactcagt accttcaggc
     3961 atcacaccgt gttcaaacaa tgacttgatt gctttggcat ccaatgtttc gaattccaaa
     4021 agtttttcag caatcagttt atgctgtgca cgatgagctt cgatgatttc tcgagcttta
     4081 tcatgagctt ccatcaagat ttttcgtact tcttgatcaa tctcgaaagc gacttgttcc
     4141 gagtaagctt ttgtttgacc atagtcgcgg ccaacaaaca cttgatggtt tccttcatat
     4201 tgaacaggac ctaaacgatc actcattcca tattctgtta ccatgcttcg tgctagagct
     4261 gtcgcttgct caaagtcatt ggaagcacca gttgattgga cattgaagat gatttcttca
     4321 gctgtacgtc caccaagtaa gcctacgatt tgttcaaaca tatcttcttt cgtcatcaag
     4381 aactgatctt cttttggtaa agcgatcata tatccgccgg cgcgtccgcg agggatgatc
     4441 gttactttgt ggacgacccg agcgcgactc aatactaatc cgacaatcgt atgaccagct
     4501 tcgtgataag caaccatttc gcgttctttt ttgctgatta cgcgatcttt tttagctgga
     4561 cctgcaatca cgcgatcttc cgcttcatcg atatcagaag catcgatttt ctttttattt
     4621 cttctagcag ctactaacgc tgcttcattc aagacatttt ctaaatctgc tccggcaaaa
     4681 cctggtgtct gttgagccac aactttcaag tcaacgtcat ctgccatcgg tttattccgt
     4741 gcatggactc gcaagattgc ttcacgacct tttacatctg gacgtccaac taaaatctgg
     4801 cggtcaaaac ggcctggacg tagtaacgct gggtctagta catcagaacg gttggttgca
     4861 gcaatgacga ttactccttc atttccgtcg aagccatcca tttctacaag caattggttc
     4921 aatgtttgtt cgcgttcatc atgtccgccg cccataccgg caccacgttg acgacctacc
     4981 gcatcaattt catcaatgaa aatgatagct ggtgcgtttt tcttcgctgt ttcaaacaaa
     5041 tcacgaacac gacttgctcc aacaccgaca aacatttcta cgaaatcaga acccgagatc
     5101 gagtaaaacg gtactccagc ttctccggca acagcttttg ctaataaggt tttacctgtt
     5161 cctggaggtc cttctaaaag tacacctgcc gggatacggg cacctaattc aacaaatcgg
     5221 cgaggatctt tcagaaattc aacaacttca actagttctt gtttttcttc ttccgcaccc
     5281 gctacatcag aaaaacgtac gcgattagct tttttgtctg cttctttggc ttttgattta
     5341 ccaaagttca tgacacggcc gccaccgcca ccgccgccgc cttgttgtcc catcatcatg
     5401 taaaagaaaa agatgatgat tacgattggc aagaagctaa gcaataggga aacccatata
     5461 ccgctgcttg attcttcttt tactgttgta gcaactttgt tttcttttgc cagttctgtt
     5521 acttggctca atgttgtatc atttggcaaa atgattgttg tgaaatgggt agtagtcgta
     5581 tctgttgaac ctaagatagc taacccaccg ctgttagcga cggtctgctg ttctttgtat
     5641 tcaccagtga tcttatatac accatttgca ggctgcactt caaattcttt gatcttgcct
     5701 gcttgtagtt gttctgtgaa ttttgagtac tcgatatctg acgatcgctg gccgttatta
     5761 ccaaaaatga aataaacgac cattaccatc gctaagatta cgaggacata atacaaggca
     5821 tttttcatca tgccgttgtt ctttttgttc atagttgccc tccctaagcg tcaatttaat
     5881 ttaacagttt tttatcaatc ctgaccattc tattgtacca taatttaatt gagatacagc
     5941 attaatttga ttgataaaca ctaggtttca atacaccgat gtacggaagg ttacgatatg
     6001 cttctgcata atccaagcca taaccgacca caaattcatt tggtacgttg aagccaacat
     6061 aatctgcttc gatatttact actcttcctt caggtttgtc aagtagtgta acaattttga
     6121 ctgattttgc tttgcggtat ttgaaaagat ccactaaata agcaagtgtt cttccgctgt
     6181 caatgatatc ttcaacgatc aacagatcgc gaccttctac attcgtatct aagtctttga
     6241 cgatttttac ttcccctgac gagactgttg cattcccgta gcttgaaaca tccatgaaat
     6301 ccaattccaa atacgtatcg atagaacgaa cgatatctgc catgaatggt accgctcctt
     6361 ttaagacacc tactactagg ggattggtgt tttggtaaat ttctgttaac tctttgccta
     6421 gttctttgca acgaacttgt atctcttctt gtgagatcaa aattctctca atatcctttt
     6481 ccaacatcag gttctccttc caattgcttc tttttgatat ttgtaaagaa gtatgtagtg
     6541 tattttatca gtttcttcac gaatactcaa atatgaaaag gcaaaaggta tcaagctata
     6601 tatatttctt ttggcatctg taatgatcca agcatgctgc ctttgtgaat taggtacttt
     6661 ctgatcgata aaatatctag atactttttt tgtgagattc tctgttaatt taatgcggtc
     6721 tcctgctttt cttttttcga catacagttg cacatcagca ggaatccaaa tgtcatgaga
     6781 aaatagagac catgaatctg ttgctttgat gtcccctgca ttttcaggtt tgaccagacc
     6841 aatccattct gtttcattca aatatattcc ttgttctgga atcagcaaca agcgctggtc
     6901 ttctgtctgg ctagttgctt gtttttcttt tttagagagt acaacaaaat gatactctct
     6961 tttcagtatc caattattgc caatactgag ttgccattgg ctttttttct gttgccactg
     7021 gtctaaaatc gactccattt gttgttcttt caaaataatt cctgtttttg agaatgcttt
     7081 gtcgaaaaag gagtaaaaaa aatagtatcg ttcagcatct gtcatttctt caatcatttc
     7141 tgcagaaaat cgaaattgtt cctcccactg ttcgacttcc ttttcgatca actgtcccat
     7201 atatttttga atgacttggt ctgcccattg gatctgttgc gaaaattgct ggaaatgtct
     7261 caacgcctgt gtattttctt gtttcaattg aggaactacc aaatgacgaa gccgatttct
     7321 ctggatatct aacaattggt ttgtctgatc ctcaaagtaa acaagctgcg attccgctgc
     7381 ataagtatat aggtcttctt tgctaaaact cagcaacgga cgaatcagtc tgcccgtagc
     7441 aaatggttgt acttctttga ttccagaaaa agtatttaac tgtcccccac gaacgatttt
     7501 cattaacacc gtctcgattt gatcatctcc gtgatgagca gtcatcaacg tgtcatattg
     7561 tccttcttcc atcacgcgag tgaattcttg ataacgaaag ttacgagctt ctgtttctat
     7621 ggcggatttg gctggatctt cccaaatact tacatataaa gggagattct tctcttcaca
     7681 atatgagcga agataggcag cttcttctgc tgattcttct cttaattggt gattgatgtg
     7741 gacgatacct aaagaaaaac cgatttggcg ttgcacttgt aaaagtaaat ggagcaaaac
     7801 catcgaatca acaccacctg ataccgctgc taaaattctc gatccttgtt gccagaatcc
     7861 ttgttcattc ccgtgccgaa taaatgactg ctgtaatagc ggtgcttttt tcccgccttt
     7921 ttcaacgttc atcctttcca caccctttct tcctaaaaaa aagagagcgg gaaaacgtct
     7981 aaaacgacaa ttaacctgcc ctctgttgat actatgaccc aaatagtcgc actatcatta
     8041 tttgcggctt tttagctgcg acgtccgcca cggccaccgc gctttccttc tgtgtttcgc
     8101 ttcaatgaag ataagcggtc atcgctatct ttcaaaaatg aactcatcaa tgaatcgaag
     8161 tcttcttttt gagctgaagg ttgactgcga gaaaaagatt ttttctgtgc tggacgattt
     8221 cggttgtcac ggggatcttt ttgttgatat tttttaggac ggctttcttc tccagattca
     8281 gcaggttgtt cctgcgcttt ccggatagat agtccgattt ttccatcatc atttactgaa
     8341 ataactttaa ctgttacttc atctcctaca cttaaaacat catggatgtc ttttacatag
     8401 ccatttgaca cttcgctaat atgaactaga cccgtttttc cagcacctag atcaataaac
     8461 gcaccaaaat ttgtgattcc tgatactttt ccttgcagtt ttgctcctac ctcgattgac
     8521 ataaaaaaaa tgttcctcct aattatttcc cttttttata ttcctgctta ttttgcagtt
     8581 gtagaactag ttgattgatc tgtttcattt gtcgagctgt ctgtatttga ttctaatata
     8641 ggataaactt tttcaccgtc ttttgagtaa aagaatttgc ttctggcaag tttagctaca
     8701 tactcatcat ctttcagcaa tgaaacatct tggtgcagct gatcaacgtc tgcgttgact
     8761 tctttcattt cagtatttgc ttcgactctc aattcgttca attgatgcat cctttgcatg
     8821 tcatggaaaa tctgcacacc tattatcgcg aaaatgacca aagcaccaac aaaaattgca
     8881 gccagtctgc gtcttcgaaa aatcagttga cgttgttgtt gctgaaattc agcatacttt
     8941 ttctttgcat attccgtatc tagcgcagcg attttattgc tttttttcgt cacttggatt
     9001 cgctccaatc tttttgattg tctttttcat tataccaata tcgtgaaagc ttgtctaatc
     9061 tatttctcta gtgtttctgt gcgattttca gaaataattt cgtacatttt ttgtgcatct
     9121 tcttttttgg ttgattcatg taattccgta attttcactt ccaacgtttt gttgccaaac
     9181 tggactttca aaatatcgcc tactttgaca tcactggaag atttcgccaa tttaccgttg
     9241 atttgaattc ttcctttatc agctacttct tttgctactg atcgtctttt gatgattcgg
     9301 gaaattttta aaaatttatc taatcgcata tcttattcct cactttttaa tttttttatt
     9361 taatcgcagg attttcttac caaaaggcaa cattagccat tctcgtactg taaacagacg
     9421 aagacgaatc actgcataaa tgaacacaga agccccgatc ccaactccta tcacgctgac
     9481 aaaaaaagca gtggaacgat gagttacagg accaaatact atattcaata caccataata
     9541 cagcagcagt actactgtca tacaaagaag gctgaacatc aacttccgtc caaaatgccg
     9601 ttctttccaa aaagcattta gctcttttgt ttcaattcga acaaaatacc aaagcgtcac
     9661 acctaatcct aaaagggtag acaggcttgc acctgctgtt cctaataaac cggtcagaaa
     9721 agaagtagtc aatcctttta ccaatagtcc ccatcctgcg ccttttaaag aagggcgaaa
     9781 cgagttcttg ctttgagcaa tactttgata agcttggatg actgcagtga aagcaatgga
     9841 aaaaacaaat aatacaagcg ctgcatttcc cgcataatct ttaaacaacg cataattgat
     9901 ataagggagc agcaaggcta atcccaaaga cgcagctaat gctaaagcgg tcgtcaatct
     9961 aagatagatt ttggctgtct gtaaaaactg acgatagatc cggttggtca aatgacgagt
    10021 caacgcaggc aggaatgtcg cacttaatgc agtggcaatc actaaaccta attgtactaa
    10081 aggctgacct cgatcataga ccccttttgc tatcttcgca ccgtattctg acagtccgta
    10141 aacttccaaa gcatttttta caaagaaaga gtcgatcagc tggaaaaaga taagcaatcc
    10201 actataaatc gaaaccagtc cgccttctac cagaaaacgc cggatcaagg gctttgacgg
    10261 ctgtttgaac aatggaaacc gtcggaagct taacgctcct ccatgaatct tttgttcata
    10321 gtagtgaagg atcccatatg cgcataaacc gccaaaaaca gctccactca ttgctgccgt
    10381 ccctgtttgg tacaccgtcc atccaagctt tctaaaagca aacgcactac agacaatcac
    10441 aaaaacacga acgaactgct caactacttg agaaacagca gttggttcca tcaagaatct
    10501 tccttggaaa ttccctcgat aaaaagatag accaggcatc agcaaaaagg taaaggaaac
    10561 tacttggatc agcggtttca attgtatgtc tcccatcatc caagcaatcc agctgctaaa
    10621 tgcaaagaca aacaaccaca aaaagacacc tgtccaaaat actaagggat aaagattttg
    10681 caaagcttgc tttcgctcga aaggattctt tctttctgca acgtactttg atatgaactg
    10741 cggaagaccg gataaagcta aagtcattgc tatcccataa attggataaa cttgctgata
    10801 aacataaaaa ccttcgtcgc cgactaagtt ttggagggga attcggtaaa gggcacttaa
    10861 tacttttgct atgaaagaag caatcgtcaa tacaaatgct ccttgcatga cgcgccgcat
    10921 ttctttatga tccaaatcat tgctccttcc tacgctgaca gatatttttg ctcacgcaac
    10981 gctttgacaa acttttggat ttcctgaagc catactgctt cttccatttg gttggggatc
    11041 gtcaagcgta tcaccatctg ttctttttca acgcctagac tggcttttag ttttgtcgct
    11101 gtcagtgctt cgaataactg ttccacacta tagcgttttg ttccaacttt acttagagta
    11161 aagactatat gcgcctcctg tttccttatc gtttctagga gcgctcgatc gccatccatt
    11221 ttgatctgcc caattgtcaa gagatgtgct acttcttctg gatattctcc aaaacggtcg
    11281 agcagatcat cttctagttc atctagcatt tcttgcgaat caagttcacg aattcgttta
    11341 tagatctcga ttttctgccg ttgatctgcc acgtatgttt caggtagata cgcatcgaca
    11401 ccgaggtcga tctcaacagt ggtcttctcc acttgactat tctttccttg tttacgagaa
    11461 acagcttcgc ttagcatttg agaatacata tcgaaaccga ctgcatcaat aaatccatgc
    11521 tgctgagcac ctaagagatt acctgcaccg cgaatggaaa gatcccgcat cgcaatcttg
    11581 aatccagatc ccagttctgt aaaatctttg attgcctgaa gacgtttttc actgacttcg
    11641 ttaaggatct tctgctgctc atacataaag taagcatatg cgacacggct gcttcttcct
    11701 acccgccctc gcaattggta taacgtggat aatcccatat aatcagcgtt ctcaacgaac
    11761 aacgtatttg cgttcgggat atctactcct gtttcaatga tggttgtagt gacaagaaca
    11821 tcatattgac cttcaataaa ttcaaaaagc gtattttcta attgcgcctc tgtcatttgc
    11881 ccatgagcat aaccgatccg tgcttccgga acgagttctt ggatttcttc tactttttgt
    11941 tcgatcgttt ctacccgatt atataaatag aatacttgtc cacctcgtcc catttctcga
    12001 tggattgctt cgcgaatagc cccaggattt ttttccataa catacgtttg gatcggataa
    12061 cgattcgcag gcggtgtttc gattactgaa agatcccgca cgcctaacat cgacatatgc
    12121 aaagttctcg gaatcggtgt tgcagtcaga gtcaaaacat ccacttgtgc acgtaactgt
    12181 ttcaatcgtt ccttatgttt tacaccaaaa cgttgttctt cgtcaatgat cagtagaccc
    12241 aaatcgctaa attcaatatc ttttgacaaa attcggtggg ttcctacaac aatatcaact
    12301 tgacctgtac gcagttgttc gatggtttct ttttgttgct ttcttgttct gaaacgactt
    12361 aatagcccga tatttacggg gaaaccttca aatctttcca gcatcgtttc atagtgctgc
    12421 tgtgccaaga tcgttgttgg aactaaaaag gctacttgtt tgctttcctt tatcgcttta
    12481 aaggccgctc gaagcgctac ttctgttttc ccataaccaa catcacctac gagtagtcga
    12541 tccattggtt tttctttttc catatcccgt ttgatctccg ctgcacttct caactggtca
    12601 tcagtttcag agtaggggaa ggcattttcg aattcttttt ggtaggcgtc atcaggtcca
    12661 aaagcatatc ctttttctga ctctcttgcg gcatataatt tgattagatc gtcagcgata
    12721 tcctcgattt ttgaagagac ttttcgcttg gttttcgtcc attcacttcc gcctagctta
    12781 ttgatgcgag gcgctttggc ttcagaggcg acatattttt ggatcagatt cagttgagta
    12841 acaggaataa acagtttgtc gtcgttttga tacaaaatag tcatatagtc ttggtgaacg
    12901 ccatctactt ctagcgtttc catcccaatg tatttcccga taccatgatt ggcatggaca
    12961 acgtaatctc ctgcttttaa ctcattatag cttttcaatc gctctgcgtt tgttactgtt
    13021 tgacgtctcg tttgtttttt cgtcgttttt tggaaaattt ctttttctgt gatagctact
    13081 attttttctt gaggaagttc aaaaccagcc tgcaaagctc cttcaactaa ttgtacctgt
    13141 cctctgatca atgcattttt ttctgtttct actactaata tgtcttcatc tcgaagcatt
    13201 tgttctgctt tgcgtattct gtcctttgtc ggaataaaga tcacaacagt ctgtttttgc
    13261 ttgcgccaac ggtccatttc tgttttcagc aatggcattt gtccgaagaa ctgttgcatc
    13321 gtccgatatt gaaaattatg gattgcttgg aaacgaagat ttcccatccc tttttggaag
    13381 agtgagaaaa aggttgtggt gaacttttcc ttctgcacga ttttttgtat atctgctgaa
    13441 aatgtctgct cagaaaacac ccgcaattcg ctgatttttt gagtctgcca ttctgccgct
    13501 tccctttcga tttcccgatt tgtttccatg attcgctgat aatcatcaac gactagtaaa
    13561 ctatttgaag ggaaataatc caagatcgtc acagattctt ggtaaagaag atctgagtag
    13621 aattttgctt cttctgtcgg gacacctgct tgccagcttg aagtcagctg accgaagtaa
    13681 tcttgcaaaa actcgcggtc tgcttcttct gtggtcgcag caagccgttt ttctagcatt
    13741 ttttgcattt gctctattcc atgttctaga tcctctggtc cgtagacttg ttcactcgtt
    13801 ggggaaaccc agatttcttc cgttttttcg attgagcgtt gtgtttctgc ttcaaaatac
    13861 cgcatagaat ctatctctac atcaaatagt tctacacgaa caggataggt tgtcgttaac
    13921 gggtaaatat cgataatgct tcctcgaata ctgaattctc ccggttttgc gaccatctct
    13981 tctcgtacat agcccatcaa agtcaattgg cgaggaagat tttccacgtc tatctcatca
    14041 ccaatatgcc aattcaattg cgcctgtagc caagttttct tattcggcag tcgtttgtgc
    14101 aaagatgcga ctggcaacaa atagatacct gctttttttt cttgaagtgc gttaagcgtt
    14161 gcaactctct cgcctcgcgc ttctggagaa gaaaaggcca tttcagcaga aagcacttca
    14221 tccactggaa ataaatggat ctgatcttgc ggaatcacat gttgcagatc ttctgctaac
    14281 tgattcgcat aatacaaatt tggcatcaga ataatcatgt ttttatttgt tttcttaaac
    14341 gctcgagcaa atagaagtgt tttcgctgag cccgctaatc ctgtgattag ttgtctaggc
    14401 attttttctt ctaaattgct gacccattct tttacttgcg gatcttttcc gactaattga
    14461 ataatatcca ttccttttcc tccttcctca attgaatcga ttcatcgcat ccacgaacga
    14521 atgcccttct atgagatatt cgactgcttt aacagattga cacatgcttt cttctatcaa
    14581 aggctgattt tctttgctga aaggactaag cacatgctgg actactgttt ttttcccttc
    14641 tggcctgccg attcccacct tgattcgatc aaagacattc gtattcaaat gggaaataat
    14701 acttttgata ccgttatgcc cgccggcact tcccttttga cggagccgga tttttcccac
    14761 tgccagatcc aaatcatcgt agattacgac taattcctct ggataaatcc caaaataagt
    14821 cattagtggg ccgactgcac gaccagattc attcatgaat gtttgtggtt tgactaaaag
    14881 gatcttttct ccattatgaa aaaattcggc aacttctgct tcgaacggat tttttttgaa
    14941 agtagcttga tgctcgcgtg caattttatc cacgaccata aagccgatat tgtgttttgt
    15001 atattggtat ttggtacctg ggtttcctaa cccgacgatc attttcattt ttgttttcca
    15061 cctaattcta aaaagcagca caaaaggctg gactgctaat tagcggtcca gtccatcgag
    15121 cttttttctt ttttatactt gtcaatgcat agtataacac aaaacaaggc gtttcggcta
    15181 aaagagaatc gttaataaat gataaagaaa ttaaaaattt cttcaaagtt taatgaaata
    15241 attgatatat aaaaatagtg aaataacgca catgtaagct tttacatata atacaacctt
    15301 gttatctgtt atattttata gccaaatagc gtgacatggg gcttgagaaa tgttagcata
    15361 agcatgggaa aaaacaaatc aatggaagga ttgatactaa atgactgcag ctgcagaaaa
    15421 aaaagatcat caaaaagtta ttcttgttgg agacggcgcc gtaggttcta gctatgcctt
    15481 tgctctagtt actcaaaata tcgctcaaga agtaggtatt gtggatatca atactgctaa
    15541 aactgaagga gacgcaattg acttgtctca cgctttggca tttacttcac ctaaaaaaat
    15601 ctattctgct acttacgcag acgcacatga tgctgactta gttgttatta cagctggcgc
    15661 accacaaaaa ccaggtgaaa ctcgtttaga cttagtaaac aaaaacctaa aaatcaaccg
    15721 cgatgttgtc acacaaatcg ttgaatcagg atttaacggt atctttttag ttgccgctaa
    15781 cccagtagat attttgactt actcaacttg gaaattctca ggtttcccta aagaacgcgt
    15841 aatcggttca ggtacctctc ttgactcagc tcgtttccgt caagcacttg cggaattagt
    15901 tgacgttgat gcacgtaacg tccatgctta catcttaggt gaacacggag attcagaatt
    15961 cccagtttgg tcacatgcaa atgttgccgg cttgcaaatc tatgaatggg taaaaaacaa
    16021 cccagaaatc gatgaagaag ctatggttaa cctattcttt ggcgtacgtg acgctgctta
    16081 cacaattatc gagaaaaaag gcgctacttt ctatggtatc gcagtagcat tagcacgtat
    16141 cactcgtgcc atcttagatg atgaaaatgc tgttcttcct ttatcagtat tcatgaatgg
    16201 cgaatatgga ttgaacgaca tctacattgg tgcacctgct gttatcaacc gtcaaggtat
    16261 ccaaaaagta atcgaaattc ctttaagtga cagcgagcaa gaccgtatgg ctgcttctgc
    16321 taagcaatta aaagaaattc tagatgaagc ttttgctaaa ctagacgctg aataatcttt
    16381 gaacatgacg gaaaacatct attcggtgtt ttccgttttt ttattttatc acccttatgg
    16441 tttctaaatt ttttcataaa gtagagataa ccttcgtctt ttcctctaaa ttatgctatc
    16501 atgagaccgt tccaccctaa acttttactt aaggagttgt cgatatgaca agatcttcgc
    16561 atcgaaaaaa atcgaaacat gttgctttgc tttcagttct tggtgttctc ttagtgctga
    16621 tcagctttta tacctatcgc agtacatact acacaaagca ttttttgccg aatacagaaa
    16681 taaatggaat aaatgtcagc aatctaacag tcgaaaaagc aaatgaaaaa ttaaaagaag
    16741 cttattctga taaaaaactg gctattaaag aagatggaaa agtctggcag gaagtaccaa
    16801 aatccgagct cggctacaaa gatgatttca cttcggagct ttctccgatc ctaaatgaac
    16861 aaaacggatg gacttgggga atgacttatg tctcagcggc agaaaaacaa gaaatagatc
    16921 ctgtcagtca aaaccaccaa aaactagaca ctaccgttca gacattgaca actaagctga
    16981 ctgatttgaa caaagaccgg acacctacta ctgatgcaac gatcgaaaaa gcaggtgact
    17041 ctttcgttat caagcctgaa gtcaatggaa atacgatcga cgtcgatgct gcggtcaaac
    17101 aattaaaaag tgcggtaaac tctggtaaag atacaattga attgactgaa ttcaaagaaa
    17161 aaccaaaagt aacatctgaa gacagctcac tgaaagaaca actcgcatcc atgaatgcta
    17221 tcgcaaatgt aaaagctact tacagtataa acggtgagac tttccagatc ccatcaagtg
    17281 atattatgag ttggctgacc tacaatgatg gaaaagtcga tttagataca gaacaagtac
    17341 gtcaatatgt aacagattta ggcacaaagt ataacacaag tacgaatgac acaaaattta
    17401 aaagtacaaa aagaggagaa gttacggtcc cagttggcac ttacagttgg acgatccaaa
    17461 cagacagtga gacagaagca ttgaaaaaag cgattctggc aggccaagat tttactcgtt
    17521 cacccattgt gcaaggtggc acaaccgctg atcatccatt gatcgaagat acgtatatcg
    17581 aagttgatct ggagaaccag catatgtggt attacaaaga cggaaaagtg gcccttgaaa
    17641 cagatatcgt ttccggaaag ccaactacac ctacacctgc tggagtcttc tatgtttgga
    17701 ataaagaaga agatgcaact ttaaaaggaa caaatgacga tggaacacct tacgagagtc
    17761 cggtaaacta ctggatgccg attgactgga caggtgttgg tatccacgat tcagactggc
    17821 aacctgaata cggtggagac ttatggaaaa cccgcggttc acacggctgc atcaacaccc
    17881 caccaagtgt aatgaaggaa ttattcggga tggtcgaaaa aggaacgcct gtattggtct
    17941 tctaacaaaa ataatatata aaaagaaagc acccgtacga ttggtacagg tgctttcttt
    18001 ttattatcta acaatgtgct acttacattt cagcagcagt aaagacttct gaaacatcat
    18061 catcgtcttc tagcttatct actagacgtt ccaattgctc tttttgttcc tcatttaaat
    18121 caatcgtcgt ttgtggaatc atcgtcaatt ctgcttgcgc caaagtgaac tctttttcca
    18181 actcatctcg tacagcagca aaatcttctg gtgcagtata aatctcgaat acttcaggag
    18241 aagtttgcag atcttctgcc cccgcctcta atacatcctc aaacatcgtc tcttcatcaa
    18301 cagtcaaccc atcgcgttca atagcgatat atcctttgcg gtcaaacaag taactgactg
    18361 aacccgtttc tcccagtgat ccaccgtttc gagtaaaagc tactcggaca tttgtcgctg
    18421 tccgattacg gttatctgtc aatgcatgga ctaagattcc tactccaccc ggaccatatc
    18481 cttcatacgt gatttcatcg taattcgttt catctacggc actcgtagcc tttttaatcg
    18541 cccgttccac attgtcgtta ggcatatttg cagcttttgc tttgtccaca acaagccgca
    18601 aagctggatt catagatgga tcaggaccgc ctgctttagc tgccatataa atttcacgag
    18661 aaagtttctg aaagactttc cctcgttttg catcttgggc attttttctt ccttggatat
    18721 tatgccattt actatgtcct gacatgattt ttcctccctt gttcttttga gaatacactt
    18781 ctattctagt actatttcac aaaaaaacaa agccacagcc aatctataaa cgcttcttga
    18841 atgccgcaaa aagcacacat aatatcacag cagtcagagc accgatcgaa tccaacatga
    18901 catcttggaa cataggtgtt cgtccaccgg tcatcatctg atggaattcg tccaaacctg
    18961 cgtaccctgt tgctgaaagc caagcaagga tcgctgtcag ccaccaaatt tttagcttgg
    19021 ggaaaacacc taaaaacaag ctgcctccca aaataaaata ggtaccgaaa tgtgcgccct
    19081 tccttaaaaa gaactctaaa aatttggcgt agcccagatg gtcgatactc accatacttc
    19141 caccataatt aaaacgtata cccgctaatg tgtctttaaa tggctgatta ggcagccagc
    19201 tttctaatcg cgaaacttgt gattgctgtc cgtaagtttg agaagaactg taaaataaga
    19261 ttataacgac tatcagtgcg attactaaat aaatatttcc gtttttcatt tgtttttttg
    19321 aagaattcat aagtcacctc tgtattttat cttagacttt ttttgaagaa acaacaatca
    19381 ttttcataga gacttcttcc ataaaaaagt gctatactta attttgtatt acaatatgaa
    19441 gggagttttt tcatgagtta ttcattaaat tggaatctag attctatttt ttccggaggc
    19501 agtcattctg atgctttgaa ccaacggatg aagcaattgg aagaccaaat gaacgagtat
    19561 tatcaccgcg taacaaaatg gtcacctagt tctgacaatg cagagcagtt aaacgctatt
    19621 cttcaattgc aagaaacgat tacgaatggt tttacccaat gcagcagcta tattactgca
    19681 ttgctttctg ccaatgtcaa tgattcagat gcaaaggtct tatcaggcaa gctttacgca
    19741 atgctgcctc gtctgcaatc agccgaaact gtattgtcaa agaaatttgc agaaatatct
    19801 gacaatgatt ggaatcagat gctttctcaa tcttcgtttg aaacaattgc ttttcgttta
    19861 aacgagattc gtcgtgatgg ctcacaatta ttatcagaag ctgaagaaaa cattataaat
    19921 acgctttctt tagatggtct aaatgcttgg agcagtcatt atgatacgat tgtagccagc
    19981 atttcgattc ccttcgaaca agatggacaa gtagtcgaat tatctgctgg acaagccttc
    20041 aataagatga tgggcgatcc agatccaaaa gtcagagaaa cacttttcgc agcatgggaa
    20101 gaagcctgga aagaaaaggc acccctcttc acggatactt tgaatcattt agatggtttt
    20161 agactttctg actatgaact tcatggtgtc acagattttc tacaaaaacc attagagtac
    20221 aatcggttga agaaagaaac attgtctgtc atgtgggata caatccagaa aaacaaacaa
    20281 ccccttgtta attacttgac tagaaaagct aatcttttcg gtaaagaaaa aatggaatgg
    20341 caagatcaag atgcacctat tattttaggc gatttgaaag aaaaaacctt tagttttgac
    20401 gaagcagcag catttattat cgaaaacttt aacaaattca gtccaaaaat ggctaaattt
    20461 gcccaatctg cttttgaaaa gagctggatc gaagcagaag atcgtcctgg gaaacgtcct
    20521 ggtggttatt gtactgagtt accagaaaca aaagaatcaa ggatctttat gacctacagt
    20581 aattctgtta atgaggttgc gacacttgct catgaactag gacatgcttt ccatagcagc
    20641 acgatgtggg atttaccttc tttaaatcgc gaatatgcca tgaatgtcgc agaaacagca
    20701 agtacattcg cagaactgat cgtagccgat gctacattaa aagtcgcaaa aacaaaagaa
    20761 gaaaaaatca atttgctaga tacaaaactg caaaatgctt tagctatgtt catgaatatc
    20821 cattcccgat tcatttttga aaatcgattc tatgaagcac ggcaagaagg attagtctca
    20881 gaagataaga tcacagagat gatggttgag gcgcaaaaag aaggctatca aggagcttta
    20941 gctacttacc atccatattt ctgggctgca aaattgcatt tctttattga tgacgtaccg
    21001 ttttataact tcccatacac atttggctac ctctttagtt tgggcatcta tgcttacgcc
    21061 aaccaaaagg gagcaaattt tgaagaagaa tatatccatt tactacgcga tactgcctca
    21121 atgacgacgg aagatcttgc gaagaaacat ttgaatgtcg atcttaccca accagatttt
    21181 tggcaagcag gtatcgacat ggtgactgaa gatatccaaa cgttcatgga attgacagaa
    21241 gaatttatct gatttctatt ttatcacaat aaaaaaagag ttagtccaga atgggctaac
    21301 tctttttgat taatcattct tacatgctga cagcaaagtc aggggcaaac cattgaactg
    21361 aaccaacttt tacgctttcg cctggttgag gagcatggat atattgtcct ccgcctaaag
    21421 caatcgctac gtggtaagtt ccgccttgtg acccccagaa taacaagtct ccagcttttg
    21481 cttgagaaac tgaaattttt gttccagctg attcttgagg aactgtccaa ccaccgatgt
    21541 cacgaccagt tacttgcatg taaacatagc gtgtgaaccc tgagcagtca aagccacttg
    21601 gatctttacc gccccaaaca tatggagtac caatatattt gtaagcttct gctacgattg
    21661 ctgcaccatt cacacttcca gaaggagctg gagcaggtgt tacaggtttt ggttgttcag
    21721 gtgttggtgt tggcgttgga gccggagtac tacttcctgt gccgtttcct gtatcaacac
    21781 tttgatctgt tgatggtgtt gtaggcgcag gtgttgttga ttcttctgtt gttgaagatt
    21841 caggcactgt tgttgattct tctgttgctg aagattcagg cactgttgtt gattcttctg
    21901 ttgctgaaga ttcaggcgct gttgttgatt cttctgttgc tgaagattca ggcgctgttg
    21961 attcttctgt tgaagattca ggtgttgttg attcttctgt tgctgaagat tgtgtagctg
    22021 atgatgattc ttcagtcatt gaagattgtg tagctgatga ttcttctgtt gctgagcttt
    22081 gtgcagtaga tgaagcttga gtagcttgtg cttcagcctc tgcttgttcg cgtgcttctt
    22141 tttcagcttt ttcttgtgca gcttgttgac gagcttgttc tgctagacgt tgttgttcac
    22201 gaatacgagc ttgctctgct tcagcttctg ctttttgacg gttcaagtca gctttcttgt
    22261 cttcagcagt tgcttgttca gcagctaatg aagttttcag tacgttcaaa tcagcttgtt
    22321 tagataaaag atcgcctttt tgagattcca aagcagcttg gttttcagct aattctttta
    22381 gtttagcatc gttttcagct tttttgtctt ctacagcttt tttatcttgt ttttgttgtt
    22441 ccatcaagtc gttgtttgct tttaccatcg ttgtcattgc ttgaacgcgt ccaatagcat
    22501 ctgctaatga atcagcattc aatacagcat cgatgtagtt tgagcttgtg ttgcttactt
    22561 gtgcttcgcg tgcttgtttt tggattgtat cttcacgttt ttcaatacgt tcttgtaaat
    22621 ctgcaatgtc ttttactaat tgtgcagatt cttgacgcaa tgtatcttgt tttgctaata
    22681 ggtcttgtgc ttgtgtgttg atttcagaaa cttggctttc taaggcatcg atttgtgatt
    22741 gtgcatctgc ttgttgattt ttcaagtctg caatcttttg atcttgctgt tgtatctgag
    22801 aatcaaaatc gtccgccgcg gcagcgatcg gtgatgctac ggcagtcaag gtcattgaac
    22861 ataccattac tgctgatatt aaactctttt tcactcttca ttcctccgac tggcttaatt
    22921 taataaatat ctaaatatat ttaagtcttt cttaacgata taaaaattgt accatagcag
    22981 tgtgtcaacg atataatagt tatgttacaa aaatgtttca aaacaacttt taaaaatgac
    23041 aaaactgttc gtttcagaaa atgatcatga aaaagccgcg ttaagtctag catttcttaa
    23101 gataacaacg attctttttc gctttcgacc taaaaataaa aaaagaaatt attcatcact
    23161 gaataatttc ttaaagggaa aaatgaataa tacaaatata atcatgttca acagcagtgt
    23221 aggtcctaaa aaccttgtaa taaaatacgt gttatttact actgctaatt taaaaatcaa
    23281 ctggaccacc atcgtaaaca attcataacc cgtgacaaag atgatcatac tgaaaaattc
    23341 agtaaagatg ttgacatgga taacgtcttt cattacatac atcaaccata caattaaagg
    23401 gagagctact gcatagatac cgataacgcc aatatagtaa gcgtcataga tagccccgag
    23461 aacaatcgtg gtaatcagta aataacgttt ttcaaacgcc atactgcaac ataataatgc
    23521 caatatcaga aagtgggcat ttgacatata ggtgcctttc gaccactccc ctagcattct
    23581 tgttaaatga ccatctatca acatcaaaaa gaacaaaacg attggcagat agtacttcat
    23641 tgtttctttc ttcaacatgg actaatcctc cgctagccgt tggacaatcg tcacaactga
    23701 cacgtcatac atttctgcat aaggtttcac ataaacttcc cgatccagcc cataactatc
    23761 tggtttcgtt ttaataaccg taccgatcgg caagttagct ggagaattcc ctccaagacc
    23821 ggatgtctgg acaacatccc cttctttgat atccgtatca ccagttaatt gggtcaccac
    23881 taaggcatgg agcttttcat catagttttt aagcaaacca aacgcttcgc cattagccga
    23941 agatacccgt actggaaaat gattggagct ttcattagag gatgacagta attcgatttt
    24001 agacgaagcc gtattgacct cgatcactcg gccaatcaat cctttttgcg acatgactgc
    24061 catatttgct tcgataccgt ctttagaacc cttgtctacg atcagcatgt cctgccaact
    24121 gtcaggcgaa cgagtaatga catttgccgt tactttttcg taactagtca gcgtctgatt
    24181 caactcaagt tcttttttca acgcttcgat ttctctctcg taattcttgt tttgctgtga
    24241 aacgtcatca tagctatcga ttttttcttt tagccgctca ttttcggcat aagttgaaaa
    24301 aagattatgg acagaatcta ctccatttcg tacccaccgt actggggcag agattgtttt
    24361 atcaaccatt gcaacactgt cattaacgat ggattggaag aaattgggct ttccttcatt
    24421 cgctcgctgc gcagcggtca cacttagtat cgtcaccacg atgataacaa taaccaaggt
    24481 aataataata tttttattag gattgaactt cttcacaaag acacctcgaa ttaattagac
    24541 tcttgtgttc aattttatca ctaaatgccg tgaaacaaaa ggtaagttgc tcctcttaag
    24601 ctttttttta gcaaataata gattctgtca tttttagcct gaaagacaaa tttttttatt
    24661 tgtttatgga tcaatctttg ctttgaataa ctgcaatcat accatctgaa aaagaaaatt
    24721 ctgcggtatc tgaaagaaat tcgttacttg cataagatgt cggatattct acttctttat
    24781 tttccaatac atacttgctg ccttttaatg taagattcga gacgggagtc aagcagcaat
    24841 aagctaaata cttcatatct ggtatctgtc ttactatatg ctctccaggc aaataataag
    24901 acaaataatt ttgacaatct ctgataataa gctgatgggc aaagggtttg aagcgaggtt
    24961 ccatacctaa ccagagattg gctaaaagat gatccaatct tccgcccgta gctccaatca
    25021 aagtgacttc cgcttgcggg taacgttcaa atgtttttaa gatacctaac tgtgtatctg
    25081 tatcgtcttt ttctgcaggg gaacgggtat actctttggc ttttgcaaat acttccttct
    25141 gctcttcgtt cgacaatgaa tcaaaatcgc ccactgccag atctactggg tagcctgctt
    25201 gaagcaggta cagcgcacca cggtctatcc cgacataggc atcgtatttg gacagcagag
    25261 actgcggcca gttttgcgga ttacctccag cagctaataa aattttcatt tagtctaatg
    25321 cctcttttaa tcgttcgatt tgtccgactg gattttcggc tccatagata tatgatcctg
    25381 caacgaagac atccgcccct gcatctttac agatttttgc tgtttctgga acgattccgc
    25441 catctacttc gattgcataa gtccaattgt tctctctacg tagatcagcg agttccgtaa
    25501 ttttttctac tgtttcttca ataaagcttt gtccgccgaa cccaggattg acagtcatga
    25561 ctagtacttg atcagccaaa ggcaaaacat gtttgatcat ggagactggt gttcctggat
    25621 tgataactac tccagcttta actcctaagc tttttgtcat ttgaagtgcc cgatgaatat
    25681 gaggagttgc ttcgacgtgg acagtaatga tatctgctcc agcttttgca aatgctggaa
    25741 tgtagttttc tggatcaacg atcatcagat ggacgtctaa cggtaattta gtaacaggac
    25801 ggattgcctg aacaatattt ggccctaatg tgatatttgg gacaaattgg ccatccatta
    25861 catctacgtg gatgtaatct gcgcctcctt tttccactaa ctcaatgtct ctttgtaaat
    25921 tggcaaaatc tgcactcaaa atggatggtg ctatcttcat tcttgtttcc tcctattttc
    25981 gtttaccttc tttttttgca tacattggtt ttcttttttc gatttcttgt aagaattgca
    26041 aataattgtc gtaacgtgtc tgtgcgatac cacctgtttc tactctatgt tttacttcgc
    26101 agccaggttc tttagcatgc atgcactctc taaatttgca atggacagaa gcttctacaa
    26161 attcaggaaa ttgtctcggc agttctcctg cttcaattgt caaaaagtca atcgagctaa
    26221 atcctggtgt atctgctact aacccgccat agataggaat cagttccaca tgacgtgttg
    26281 tatgccgtcc tcttccaagt gcatcagaaa tctctcctgt tgctaaattc agttccggtg
    26341 atatttgatt cagtagcgtc gattttcctg ctccagactg acccataaag accgatagcc
    26401 gttctgggaa gtaacggatc agttctgtcg tatcacccgt atgattgggt acaatcaccg
    26461 gatagcctat tttagtatag gtttgcttga tctcttcgat tttttctggg tcgtctgaca
    26521 agtcggtctt tgtcaaaaaa ataatcggtt cgatcgactc gtattccaag gtaactagaa
    26581 agcggtcaag tagattataa gaaaatgcag gctctacgat acttgtgaca atgattccaa
    26641 gatcaacatt tgtgactggt gggcgaacta attgattttt tctcggcaag atttccagaa
    26701 gatagccgtc tgtttgattt tcactctcaa agatcacctg atctccgaca agcggcgtga
    26761 ttttccgatt ccggaaattt cctcttcctc ttgtttgata ggtctctccg tttgcgtata
    26821 catagtaaaa accgctgatt gcttttctga tttgtccttt gataagtgcc acctccgttt
    26881 ttgtttattt taccatacga agaggaaagt aggtgttatt ccatgcaaac ttctcaaagt
    26941 atatggcttg aaaattgatc aaaagagata gaaacacaaa aaagagaatg caacaaatca
    27001 atgtcgcatt cccttctgat atattttatt cagcacttat tttgaacttt ctgaggtgct
    27061 agctgatgaa cttgtggagc tggaagattc ttcttttgaa gagttgcttg tgctactgga
    27121 gctacttgag ctgctagaac tgtctgaaga gtctttttca gaactagaag acgctggatc
    27181 tggtccttta gagtaaatta cgttcacaac cattcctttg gtaattgtcg ttcctgcccc
    27241 tggtactgta cgaatcactt tatctttatc caccgtgttt gaatattcgg aagtctcatg
    27301 cccgatatat tctgcaccga cactagctaa ataactttgt gcttcgcttt ttgtatagcc
    27361 ggagatatca ttcaatttag gctctgttcc ttggcttacg acaaaattga tgtaagtttc
    27421 ttctgctaca acttcacccg aggctggatc ctgactgatg atcgaacctg atggtacagt
    27481 gtcgctatag tcataagtag cactaatatt attgatattg aatccttgtg taacaagcca
    27541 attacgaaca gtttcgtagt tttctcctac atgactactc aaagtcactg tttctggtcc
    27601 tttgctgact gtgaaagtaa tcgtgtctgt tttaggatcg acttctgtat cttctgatgg
    27661 agattgcgaa atgatcattc cgctttctac ctctgaactg aattcctctt ctttttgaat
    27721 caagtcagca gaaaacccta attctttcaa acttgcttcg gcatcttcat aacttttacc
    27781 actgtaatca tctaatttga tttttttcgt gccgctacta atatacagag tgacttcgcg
    27841 tttttgcttg actgtcgttc ccgctgccgg gtctgtcttc actacattac cttcttcgat
    27901 tttgtcatct gcaatttctt ctgtttttgt tgctacttct aatttcgctt ttgcgagtgt
    27961 ttctctagca gaagcttcag aaagatcaga aacatcagga acaacaactt ctcctcgccc
    28021 gccagaagca aagaatacag ctgttccgat tcctaaaaca gcaagaatag ctaataggat
    28081 cagccaccat tttttctgtc ctttcttttt aggatcaggc agaacttctt cctcctctgg
    28141 caaggtttcg ttttctggct tgtcttttgg cagaggcata gatttgaatg aatcaggcat
    28201 tggtgcatct tccgtaaggg gtgtgataac ttttgtttca ttatccatca catgaggctg
    28261 ccatttttct tcgtttgcac gagctggtga taatactgta gaaagatcat cagacatttc
    28321 ctcggctgac ttataacggt ctgcaggttc tttagccgtt gcccgtaaaa tcacgttttc
    28381 cagtgcttgt ggcacatttg gatcaagtgc ttttatagat ggaagatcat cttgaaaatg
    28441 ttttaaagca attgttacag cagattcacc atcaaacggt acgctacccg tcaacatctc
    28501 ataaaggatg atccctaaag cataaatatc tgactgcttc gttgccatac ttcctctggc
    28561 ctgctcaggg gacagataat gaacagatcc cagcatcgta ttcgtctgcg tgatcgatgt
    28621 ttcagataaa gcgatagcaa tcccgaaatc cgtgattttt acaacacctt cattatcgat
    28681 cagtacgttt tggggtttca aatctcggtg gatgatttga tggttatgag ctaaagaaat
    28741 agcagaaagg atctgctgca taatatttac tgctgtttca taaggaacag gataatgtgt
    28801 ttggatatat cgtttcaagt ccattccttt gacgtattcc atcaccagat actgcatgtt
    28861 gtcttcttca ccaacatcgt aaacactaac aatgtttgga tgaacgagtt ctgttgctgc
    28921 taaagcttct ctctgaaatc gtcgaatcgc agtctggtca ttttgaaagt caaatcgtaa
    28981 gactttaact gctacatcac gatccaagat taaatcgtgc gccaaaaata catttgccat
    29041 accaccgcta ccgatatttc cagtgatttg ataacggcca tttaattttt tgcccaactc
    29101 gatcatacac tgtcctcctt atattcgata agaagaacgg tgatattatc cgctccgcct
    29161 gcttcattgg cacgatcaat cagctcagtt actttatgag acaatgggcc ttcttgattg
    29221 atgatcgttc ctatttcttc ttcagacagc atgttcgtca agccatctga acataacagc
    29281 agtcgatctt ttagttgcca aagatgagta gaaacatcta cttcgactgt tccaggcatg
    29341 ccgacagaac gtgttaaaat atttttcctt ggatggttca ctgccatttc ttcgctgatt
    29401 tctcctgatt tgactagttc attgactaaa gaatgatcct ccgtcaattg catgatttta
    29461 tcttcacgaa tcaaataagc tctgctatcc ccaacattgg caatcgtgaa ccagtcatct
    29521 gacagagcgg ccgctacgat tgttgttccc ataccgttat attccggctg ttcttgtcca
    29581 cgctgataaa tcagcatatt ttcttcttga atcgattgaa tgaaccattg ggcgattttt
    29641 tcatcgtcaa cagcttcttg tttttcccaa gcttcgccta gattcgttac cgccatctga
    29701 ctggcaatgt ctccggcacg atgaccaccc atcccgtctg ctaatacagc aagcttgaga
    29761 cctgaacggc tggtaaatac actggcatag tcttgatttg tgttacgtcg tcttcctaca
    29821 tctgattgat attcaatctg cattttttca cctcattact ttttgcgcaa acaacaaatg
    29881 aaaaatccat ctgtgtaaaa ctgatgcgga tacaaagtca acattttctc ttcgattgat
    29941 gattgtagca taggattggt caaaacatca atcctctcaa aatcagggtg tctttccaag
    30001 aaagcagaaa caacgtcttg gttttcttct tttaagatcg tacaagtgct ataagtcaga
    30061 ataccggaag actttaaagt tggtgcgcag ctttctaaaa tttccaattg gatttttggt
    30121 aaattcgcta attcatccgg ctgtttttta taacgaatat ctggttttcg ccgaagcagc
    30181 ccaagacctg aacaaggcgc gtctactaaa atccgatcaa actgctctgc ttcgaactgt
    30241 ttactgactt ctctagcatc catcttttga gcgtagacag cagaagagac acctagacgt
    30301 gctgcattat cttcaatgag tttgactttg tgttcgtgta catctaaagc gaccacttct
    30361 ccaccgcatg acggatctaa aaaagaagca atatgcgtcg tcttacctcc gggtgctgca
    30421 caggcatcta agacatgatg ttcaggctcg atttgcatag acggcgctac aagcatcgaa
    30481 ctttcatctt ggatcgtcat gacgccatcg tggaacaatg agctagaagc taaatgccct
    30541 tttttcgcta ctataccgta tggactgact tggctttttt cagctgtgat ctcttcttct
    30601 tgcaaaacag tgatggcctg atctctggaa agtcggcgtg tatctactcg ggcactagca
    30661 tggctaggct gatatagcga taaaccgagc ttttcagttt cttcgtatcc gatttgatcc
    30721 accagtcgtt tcgtcatcca tagaggcagg ctgattttga cagaaagtct ttcgatagga
    30781 tcagcaatgc cttgcagatc tggtgctcct tgacgctgat aattcctcaa gacaccattt
    30841 acaaatttcc ctgtcccagg atttcctttt acttttgcga tttcgaccgc ctcgtttaaa
    30901 atagcatgtg cagggacttt atctaaaaat tctaattggt atacagaaag ataaagaagc
    30961 gtcttgaccc agtcatccac tttttttgct ttagcgatca atggtgcaac gtaaaattcc
    31021 aatagtcgct gatggctaat cgtaccatag atcaattctg taaataaacc cacatctttt
    31081 tgatttagct cgctttgttt gatcatctca ttcaacaaaa gattcgagta ggcgccaccc
    31141 ttgttcactc gctctaatgt cagtaatgcc atatatcgta ctgatttttt cattttttta
    31201 gggatttttt tcattatttc acctgctgac caatctgaac ttgctgaccg cttccgttaa
    31261 ggaaatcatg tacggcctgt tttcctttac cagctggctg taattcattg atggctaaga
    31321 cggtctgttt accgcaagca atccatagat tttttttgtc tttccggatg atcgtaccag
    31381 gtgctttgtc tgtagtttct gccaatactt caaccgataa taatttccag cgtatgccct
    31441 cataggtcgt gaatgcaatt ggccaaggac gcattccgcg tacttggttg tcaatttctt
    31501 ctgcagtttt atgccaatcg attgcttctt gttcccgtgt gatgtttggc gaaaaagttg
    31561 cttctgactc gtcttgaggt actggttttt gtccatccaa aatattgggc aatgtttcta
    31621 gcaatagctt tcttcctaaa aggctaagct tttcaaacat cgtccctaca tcatcttgtt
    31681 tagtgattgg tatgctttct tgcgcataga ttccgcctgc atccattttt ttgatcattt
    31741 ccatgatcgt tacaccagtt tctttctccc cattcatgat ggcataatga acaggcgcac
    31801 ctccccgata tttaggaagt aaagaagcat ggacattgat tgcccctaat tttggtactt
    31861 gcaataactt ttcaggcaag aattggccaa atgccgcagt gacgatcaca tcaggcacca
    31921 attcaatgat ttcttccatt tctggcgaac cggagatttt ctctggctgc agcacacgga
    31981 taccgtgctt cagagccgct tcttttaccg gtgtcggcgt aattactcgt ttccgcccaa
    32041 ctggacggtc gggctgtgtt accactgcct ggatctcgta acctgattct atcagccctt
    32101 caagaatcgg cacagaaaaa gcaggtgtgc ccataaatac tagcttagtc atcaagatgt
    32161 tcctccatat attcttcaag ttcttcttcc gggatctgct gtatcatttt ctcaatgaac
    32221 aagataccgt ctaagtgatc gatttcatgc tggaaggcac gggctaaata gccaaaagcc
    32281 gtcacttcta tctcttctcc gtctcgatca taataacgca cggtcacttc atctgctcgc
    32341 ttgaccgttc catagacatg cggaatactg agacaacctt ctacgtcaag gctttcacct
    32401 tttgcttcga tgatttctgg gttaatcagt tcaaatttct cgccttcatc tacttcgatc
    32461 acagcaattc tcttgttttg tcctacttga ggagcagcga tccctattcc gtcattcgca
    32521 atcattgtct catataaatt gtctaataaa gcgattgtct cgtccgtgat tacatcgatt
    32581 ggctgagcag ttctttttaa tttatcatta gggtggatca atataggata gcgcataatt
    32641 tggccctttc ttaaataaag tttaatggtt cgttgtcaat tgccacaaaa agaccttgtc
    32701 gctgttcttt ctggcttcca ttcaaaattt catccaaaac atgaggaaga ttcggttcat
    32761 gtttatattt gatgattagt tgataataat atcggttttt cactctcaaa atcatgctgg
    32821 gcgttggccc taatagcaag ctttgagggc ttaacacttc ttttagctga ttggcaatct
    32881 gaaagatttt ctttgccgct acttgttctt caggatggct ggcggttatt ttcacagtaa
    32941 agtaataagg aggatagcct ccacgatgcc gtacatgcat ttcctgctga taaaactgct
    33001 cataattttg ttttttagct aattctatgg cgtaatgttg aggattgaat gtttggatca
    33061 cgacttcgcc tgctttttcc gctcgccctg ctcgtcctga aacttgggta agcaattgga
    33121 atgtatgctc actagaacga aaatccggta aattcaggga ggtatcagca ttcaacaccc
    33181 caactaatgt cacttctgga aagtccaaac ctttagcaat catttgtgtc cctaataata
    33241 tatctgcttc ttttgcaccg aatttttgta aaatctgttc gtgtgcccct tttcttcgag
    33301 tggtatctac atccatacgc aaaatcctag cttgcggata aagttctcgg agttcctctt
    33361 ctactttttg agtacctgtt ccatagtagc ggattttatt gccgccacaa ttaggacagc
    33421 gattgggaat aggttcttca tgtccgcaat aatggcagcg cattgaacgt gtatccatat
    33481 gcagtgtaag agagatatcg caattaggac atggaagaac aaatccacag tccctacaca
    33541 tcacaaaaga cgaatatcct cgacgattga gcagtaggac agtttgttct tttttgtcta
    33601 atcgattttg tattttttcc tgcaaattag cagaaaaagt agaggtccga tgattctgaa
    33661 actcttcacg catatctacg atctcaatag ctggaagctg tgcagctgct ttggctcggt
    33721 ggtttaactg caataactga taaacgcctt tttgagctcg tgctcttgat tctaaagagg
    33781 gcgtcgcgct tcctagaact attgggcaat gatgatattt actgcgccaa attgctagat
    33841 ctctcgcatg ataacgcggt gtctcatctt gtttgtaact tgattcatgt tcttcatcga
    33901 taatgatcag cccgatattt ttcagcgggg caaaaatagc agatcgtgcg cctactacta
    33961 cttgcgcttc ttcccgctca atcttgcgcc attcatcata tttttcacct tgggaaagtc
    34021 cgctatgtaa gaccgcaact gattctccga agcgtccttt gaatcgctcg accatttgcg
    34081 gagtcaatgc gatttcaggc actaacataa tagccgtttt attttctgac aaaacatcag
    34141 cgattgcctg aagatatact tctgttttcc cgctgccagt gattccctcc agcaaaaaga
    34201 cctgatcttt ttgctgctga cctgctgtga tgatttgttc gacagcattt ttttgttctg
    34261 cattcagttc caatgccgtc gtttggtcaa atactcggtc tttataaggg tcgcgataac
    34321 gttctgattc tataaactcc agccagccct tttcttttcc ttgattcaag acagcagtac
    34381 tgattccttt atttttgaaa taagcaattg gttcttcttg gctcagttca gataaacaca
    34441 aaagaagttc tttttgtttc ttggcatttt gccgtaattt tgcccattct tcttcgattt
    34501 gcttttcagt taacaaagaa cggaccaaac gaatcgtttt tactttattt cgtgtatgga
    34561 cttcatattt gatatctact aattgctctt ggcgccagcg aatcaattga gaaaggctgc
    34621 cttcttcttc tgcttccttc caggaaatca cttctcgctg atcaaacaat acctgaactt
    34681 ctggtgtgtc tgccaaagga tagatcaatt tatcatattc tgctttcatc acactcggaa
    34741 gcatcgtttg tagacaagtg attttgaatg cgtaagtaat ttttttcata taatcagcta
    34801 atgaaagtaa ttcttcattg accaccggag caaggtctag caaacgaata actgccttca
    34861 aagatggatt agctgattct tctgattttt ggatagccat aacaaatcct tggacatgac
    34921 gattgccatt accaaaaggg acttctactc gcatcccgac ttgcaaagct gtttccattt
    34981 cagagggaac caaataagtg aatggctgat cggtctgcat agtaggtacg tccacaatga
    35041 cttcagctgt taatgtcatg gttcgtttcc tccttttttt gtcttttctt atcaagcgac
    35101 aaaaagttcc ttgcctattt tactagagat cgatggattt gtcttatctt ctgccaaatc
    35161 ctaacaaaaa attaacagca gataagaaaa aaaagaactg tgtcaaaaag ttcagacaca
    35221 gccttttcta ccagacaata agtggcgaat gtttgaaaag gaaacagctg ggtttgcttt
    35281 tctttttaga tgttcgctct atttttcttt attacatttt ttgctcttca cggatacgtg
    35341 attccaattc gtgttgttca cgctcttttt gtgcttgacg ttcctcttct tccatcttca
    35401 aacgttcacg ttttaattca ggatgtggat cattgactac atttcctgca tcgatttctt
    35461 ctaaagcttg accgacgctt tttactgaat caaaagaatc tagagtaggc tgtgcacttg
    35521 catccaattc atgcgcgcgt ttacttgcta aaatcactag agaatatttt gaatttacac
    35581 gatctaatag tgagtcaatt gaaggtttta acatcataag actacatctc ctttaacatt
    35641 ttaatatatt taccgattac gcgatctact cgaaaatgtt cactggcaat aatgtttttg
    35701 attcgttcaa ctgctagcgg cacttcatca tttacaacag catagtcgta aagagccatc
    35761 atttcgattt cttcttttgc aactttcatt cgttcttcga ttacttcatc tgcatctgtt
    35821 cctcggccta cgatccgtga ttttaattcc gctaaatctg gcggagtcaa aaagatgaat
    35881 acaccatcgg gcactttttc tttgacttgt tttgctcctt ggacttcaat ctccaagaaa
    35941 acatcttttc cttcatccaa tgtcttgttg atatatgaca aaggagttcc ataataattc
    36001 ccgacatatt ctgcgtattc cagcatctct ccagcttcga tcattgattc aaactcttct
    36061 ttgcttcgga agaaataatc aacgccttcg acctcccctt cacgcatttt acgggtagtc
    36121 attgagatcg aatactgaaa atcattatcc tcactctcaa aaattgcttt tcgtactgtg
    36181 ccttttccta caccggatgg accagacaac acgattaata atccacgctc tgacatgacg
    36241 acgtcctttc aactagtctt tttgtactta tcgtttattt tgcacttttt ttctgcaact
    36301 ttcaaggtta tctttaatta tagctagaaa ttggtaaaaa agctaccctg taccaagagg
    36361 tcgtaaaaaa gctgtcctgc atcaagtaat aagaacagcc aaacataaac agcttctcat
    36421 gtttttcgtt tggttggggt tattatcgca gatgcaagct tttgaacgcc gtgtataaga
    36481 ggtcgtgaag aagccgatta gtttcgagta tgaagaaaaa ttttgtgaaa atagtttctc
    36541 acatttttgc ataaatttgg cttaata
//
LOCUS       DACGLW010000018        35318 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.018, whole genome shotgun sequence.
ACCESSION   DACGLW010000018 DACGLW010000000
VERSION     DACGLW010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35318
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.018"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            208..1014
                     /gene="proB"
                     /locus_tag="IXT53_000975"
     CDS             208..1014
                     /gene="proB"
                     /locus_tag="IXT53_000975"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HAQ4904821.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            1011..2258
                     /locus_tag="IXT53_000976"
     CDS             1011..2258
                     /locus_tag="IXT53_000976"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HAQ4904822.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            2334..2678
                     /locus_tag="IXT53_000977"
     CDS             2334..2678
                     /locus_tag="IXT53_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4904823.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(2795..3121)
                     /locus_tag="IXT53_000978"
     CDS             complement(2795..3121)
                     /locus_tag="IXT53_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4904824.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(3494..4695)
                     /locus_tag="IXT53_000979"
                     /pseudo
     CDS             complement(3494..4695)
                     /locus_tag="IXT53_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            4812..5327
                     /locus_tag="IXT53_000980"
     CDS             4812..5327
                     /locus_tag="IXT53_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ4904825.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(5526..5972)
                     /locus_tag="IXT53_000981"
     CDS             complement(5526..5972)
                     /locus_tag="IXT53_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAQ4904826.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(6019..7497)
                     /gene="msr"
                     /locus_tag="IXT53_000982"
     CDS             complement(6019..7497)
                     /gene="msr"
                     /locus_tag="IXT53_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAQ4904827.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(7851..8480)
                     /locus_tag="IXT53_000983"
     CDS             complement(7851..8480)
                     /locus_tag="IXT53_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ4904828.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(8464..8928)
                     /locus_tag="IXT53_000984"
     CDS             complement(8464..8928)
                     /locus_tag="IXT53_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ4904829.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(9415..10683)
                     /locus_tag="IXT53_000985"
     CDS             complement(9415..10683)
                     /locus_tag="IXT53_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAQ4904830.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(10914..11651)
                     /locus_tag="IXT53_000986"
     CDS             complement(10914..11651)
                     /locus_tag="IXT53_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ4904831.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(11718..12287)
                     /locus_tag="IXT53_000987"
     CDS             complement(11718..12287)
                     /locus_tag="IXT53_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAQ4904832.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            12415..13287
                     /locus_tag="IXT53_000988"
     CDS             12415..13287
                     /locus_tag="IXT53_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ4904833.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            13629..14501
                     /locus_tag="IXT53_000989"
     CDS             13629..14501
                     /locus_tag="IXT53_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ4904834.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            14498..16456
                     /locus_tag="IXT53_000990"
     CDS             14498..16456
                     /locus_tag="IXT53_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ4904835.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(16707..17558)
                     /locus_tag="IXT53_000991"
     CDS             complement(16707..17558)
                     /locus_tag="IXT53_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ4904836.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            17681..18148
                     /locus_tag="IXT53_000992"
     CDS             17681..18148
                     /locus_tag="IXT53_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAQ4904837.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(18411..18893)
                     /locus_tag="IXT53_000993"
     CDS             complement(18411..18893)
                     /locus_tag="IXT53_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ4904838.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(18993..19856)
                     /locus_tag="IXT53_000994"
     CDS             complement(18993..19856)
                     /locus_tag="IXT53_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ4904839.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            20454..21302
                     /locus_tag="IXT53_000995"
     CDS             20454..21302
                     /locus_tag="IXT53_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4904840.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            complement(21473..21814)
                     /locus_tag="IXT53_000996"
     CDS             complement(21473..21814)
                     /locus_tag="IXT53_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HAQ4904841.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SMEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAAEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            21900..22607
                     /locus_tag="IXT53_000997"
     CDS             21900..22607
                     /locus_tag="IXT53_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAQ4904842.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            22594..22917
                     /locus_tag="IXT53_000998"
     CDS             22594..22917
                     /locus_tag="IXT53_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAQ4904843.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(23006..24043)
                     /locus_tag="IXT53_000999"
     CDS             complement(23006..24043)
                     /locus_tag="IXT53_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ4904844.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(24490..25473)
                     /locus_tag="IXT53_001000"
     CDS             complement(24490..25473)
                     /locus_tag="IXT53_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HAQ4904845.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
     gene            complement(25889..26659)
                     /locus_tag="IXT53_001001"
     CDS             complement(25889..26659)
                     /locus_tag="IXT53_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ4904846.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(26652..27587)
                     /locus_tag="IXT53_001002"
     CDS             complement(26652..27587)
                     /locus_tag="IXT53_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4904847.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(28181..29830)
                     /locus_tag="IXT53_001003"
     CDS             complement(28181..29830)
                     /locus_tag="IXT53_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAQ4904848.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            complement(30025..30921)
                     /locus_tag="IXT53_001004"
     CDS             complement(30025..30921)
                     /locus_tag="IXT53_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ4904849.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(30914..31594)
                     /locus_tag="IXT53_001005"
     CDS             complement(30914..31594)
                     /locus_tag="IXT53_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ4904850.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(31595..32377)
                     /locus_tag="IXT53_001006"
     CDS             complement(31595..32377)
                     /locus_tag="IXT53_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ4904851.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(32370..33287)
                     /locus_tag="IXT53_001007"
     CDS             complement(32370..33287)
                     /locus_tag="IXT53_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ4904852.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(33290..33574)
                     /locus_tag="IXT53_001008"
     CDS             complement(33290..33574)
                     /locus_tag="IXT53_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ4904853.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
     gene            complement(34275..34895)
                     /locus_tag="IXT53_001009"
     CDS             complement(34275..34895)
                     /locus_tag="IXT53_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4904854.1"
                     /translation="MNINRVIKLIGAKNINKVRSEFIFPEDFFNWFFYQFSECKRLLG
                     ENYYLKGIGGFMGNVADEQNIIKGISEQTSELTVTKAFISNGESLKNVTARIRKENEV
                     DIVFGIDHECNSVIYVTQSANLILFENGCKDDFMVVYLYDILIPKLRSLYMSSESIFV
                     SEQKGSFSKKIGIEVIESIMCHNHITLEELEILIGQKNNNTKIQVS"
     gene            complement(34976..>35318)
                     /locus_tag="IXT53_001010"
     CDS             complement(34976..>35318)
                     /locus_tag="IXT53_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4904855.1"
                     /translation="NIKTSTKLICKLFKLDKISNIDDVKSKILTRKVSEPVKLTAQDK
                     KNGIVLYGDDAKYNYNWDTSSEAEVLSIPTTDTSGEKVTFKINFITAISKLEYGKQKK
                     RDNNDQYLPKK"
BASE COUNT        10574 a         7352 c         6243 g        11149 t
ORIGIN      
        1 tactcctatt ctaacatatc cgaggctgta aaagactttc tttacaaatt cttcaagaaa
       61 ttctgctatc ttaaagtaaa ccattaattc gttctttttc caaacgactg caagttgtac
      121 aaaaaaacta ttttttacat gactttactc gtcaaaacaa gtagaataga acgaaattaa
      181 tgagtggata tttgattgag gtgtcacatg cgtaaagaaa ttaaaaatgc aaaacgaatt
      241 attgtcaaag taggaacaag tacattgatt tatcccaatg gaaatatcaa tcttgcggca
      301 atcgatcagt tagcttttgt cctttctgat cttagaaatc ggggaaagga agtgatctta
      361 gtctcatcag gagctgtagg cgttggtatg cataaattac atttgtcaga acgtccgact
      421 ttgattcctg aacagcaagc tgcggcggct gttggtcaag ccgaactgat gaatatctat
      481 aatcaaagat tcagtgtata taatcaacag acggcacaga tcctcgtgac aagagacgtg
      541 gtagattatc ctgaaagtcg taaaaatgtg aggaatacat tggaacagct tttgattatg
      601 gacacgatcc ctatcgtgaa cgaaaacgat actgtagcag tggatgaatt agatcatctg
      661 acaaaatttg gcgataatga tcagctgtca gccatcatgg caaaacttgt cgcggcagac
      721 cttttgatcg tcctttctga tatcgacggg ttttattcag ataatccgac tacaaatccc
      781 gaggctcaga tgtatcgcta tatcgataaa atcgatgaaa agattctggc acaagcaggt
      841 ggtgctggta gcacatttgg aacaggcggt atgcagagta aattaaaagc cgcttctagg
      901 atatttgaag caaacagcgc aatgatttta gcaaatggaa aaaatccggc aattattttt
      961 gatattcttg caggaaaaga agtaggtact ttatttaagg aggacttgaa atgactgact
     1021 tgatgcaatt aggacaacag gcaaaagata gcgcgcaaca actagcactt atgcctacaa
     1081 accgcaaaaa tgagctactt gttcaaatgg cacaaaccat caaaaagaat caacaagcga
     1141 tcattgaagc aaatcataaa gacttagaaa aagcagtcga aaacaatatc agtgaaacga
     1201 tgcaagaccg cttgcagctg acacctgagc gaatcaatgc catggctaca gagatcgaaa
     1261 aaattgcttc gttagacgat cctattggaa aagtagatga gatgtggacg aacaccgacg
     1321 gattgagaat cggcaaaaag cgcgtgccac taggcgtgac cggcatcatt tacgaatctc
     1381 gtccaaatgt cacgacagat gcagctagtc tagcattcaa atcagggaat gccgttatcc
     1441 ttcgcggtgg caaagaagca ttcttctcga atcaattatt agtccaatta ttgcagcaag
     1501 tactggtcag tgctggcgaa tctccttatg ccatccagtt tgtcgatgat acttctcatg
     1561 aaactgcagg acagttgatg cgattgacag aatacttaga tgtgctgatt cctcgaggcg
     1621 gagcaaaatt aatccaacga gtcaaagaac aggcaacggt acctatcatt gaaacaggta
     1681 caggaaataa tcacgtatat atcgataaag aagcacagtt atctatggca gtgaacatca
     1741 tcgtcaatgc aaaagcttcc cgtccatctg tctgtaacgc ggcagaaaca ttgctgatcc
     1801 attctgagat cgcacctttt ttcttgccag ctattgaaaa agaactggta gaacacgggg
     1861 tctctttacg tgcggatgct cgtgcgttag aatatctaga gacggctgct ttagcagaag
     1921 acacagattg ggatactgaa tatctagatt atatccttgc tgtcaaagtg gtcgattctt
     1981 tggatgaagc aattgcacat atcaatcggc ataatactaa acattcagaa acgatcgtga
     2041 cagatagtta tgcggctagt caacgtttct taaatgaagt ggacgcggcg gctgtctatg
     2101 tcaatgcctc cactcgtttt acagacggat cggtatttgg ttttggtgca gagattggta
     2161 tctctactca aaagttgcat gcccgtggtc caatggggtt aaatgaatta acttccacaa
     2221 aatatatcat ttatggcgat ggacaaatcc gcgaataatg tttcgtgtga aacattattc
     2281 ccgccttttg ccttttttct gtaaaggttc tatccaacta gaaaggtgtc aatatgtttg
     2341 aaattttaat gatttttttt atttacctta tttctctgaa tatcgctgct tttctgggag
     2401 tcggtatttt atcccttttc tttcaattca aaaaacgaag catagggagt caacgggaaa
     2461 aatggtcgca gtattttgac aaaatcgggc aaaaagggct ggttacacgc ttgcacatta
     2521 gttacatggt tgcattatgc ttattagcga caatcaatta ctattctttt ttcgatcatt
     2581 caatcgctta tacgattacg ctactgattg ctgggatttt tcacttatct tataaatatc
     2641 agctgaataa aaaccacttg aatcggactt tccgctaaaa aatatccgta cgataagtag
     2701 agtgccttga agatcggcaa aaaactgccg gactttttag aggctgtgac aaagattttg
     2761 tcacagcctc tttttccata taaacggcgt tcgcctacca gctgcctggt aaatctgttt
     2821 cctcttcgtt aagaagcgaa aatccgtcaa atgtttcgcc atcataataa cgaagcagct
     2881 catccatgga caagttcatc gttttttctt tttcttcttc cgctgattca gccagtagct
     2941 gttcccgata cacctgatac ataccagcta ttttttgctg catcgctttt ctagtttcac
     3001 cttccgcatt gattattggc aattctttga tatagccacg ataaatcaac cgatcatctg
     3061 aaaattcaat gggtaattca acgagtgtca acccgacttc ctgaaaatat tcttccagca
     3121 tctattatcc ccccattctt tttcttaaat gaattttagc ttacctactg aaaattttcc
     3181 agtttcatta ttttgagaaa aaaagggaat gtgacaaaaa tcgtgtcaca tccccttttt
     3241 ccgaataaat ggtgttcttc tgccaactcc tcaatcataa gtcacaatat tcaacaaaca
     3301 tgagaaactg ttttttgaaa ttgttccttg tgattcagag ttaaacgaca gtctcacaac
     3361 ctcttggtaa acggcgttca aaagcttgca tctgcggtaa taagcccaac caaacgaaaa
     3421 atatgagaag ctatttttgt ttggctgttc ttatgacttg atgcaggaca gctttttcac
     3481 aacctctgat ttcttattct tcttcatttg tagcaaaata attacggtga taaatgatac
     3541 aacatacact gataagaaca atggcacctg caaaataaaa cgcatagctt tcaccaatat
     3601 gttcaccaat cacacctgca ataaatggtg ctaaagctcc accgaaaaag cggacaaaac
     3661 tatagctgga cgaagcaacg tttcgttgca gacctggtat ttccatagca atcgtcgtca
     3721 acagtgtatt gataattcct tggaaaaatc ctgctaacac aatgctgatc ggtacaagga
     3781 ctggcataga agttccagct cctaataaca gcaaacaaat aatgaataag acaaatgctg
     3841 ctaaaattgt ccgataagtc gtaaacttcc gctcaagagc cggtgcaacg aaaatcgaag
     3901 caatagccag aagcacaccc cagccaaaaa atacaaaacc gacttctaat tcagaatagc
     3961 caatcaataa aaatggcgca tacgcaagta aagtaaagaa tccgaaatta tataacaatg
     4021 cgattagtcc aactgaacga agtttatgat tgcttaacgc tttgacacct tcccaaaggc
     4081 ctacttttct ctttggtttc tcagtttcag gtaaaagtac cgctagggct ataaaaccgc
     4141 aaaacatcaa aaatgctacc ccaaaaaatg gataacgcca tgaaccactg cccaagaccc
     4201 caccgactag agggccaaca gccattccgc agccaagtgc tgcttcatac atcatgattg
     4261 ctttttctgt gttaccaact aaaatactga cgatcgttgc taaagcagta gagacaaata
     4321 aagcattccc gacaccccag ccggcacgta acccaatcag cattccgata ttcccagata
     4381 atccgccaag accagcgaat acaacgatga taaacaatcc tgtcatcaat gttttttcaa
     4441 accgatccga ctagataaga aacctgtaaa tagcatcacc acaccagtga ctaacatgta
     4501 actagtaaat aacagggtcg tttcagctgg cgtagcgttt aactgtgcag caatcgtttt
     4561 taagattggg tctactagac ccactcccat aaaagcgatc atacaggtag ctgctacaac
     4621 tagcgctgaa cttttcccct ttgtttcttc atttgtttgt tcttgtatat ctatttgatt
     4681 ctccaatatt tccatccctt tctctctatt agcatcatac atgatttatt ttacatgtaa
     4741 atatgcacat gtaaaacgtg gaaactttac tgtgatataa taaagaaaat aagacgagag
     4801 gtaggatttt tatgaatact ttagcctata tattattatg cgtgctttta tccaaaccac
     4861 gttctgggta tgaattgaag cagctgatca caattttttg ggaagcccat cacagtcaga
     4921 tctatacaac gcttgccaag ttagaccagc agggttatgt gaccgttttg gacaaagacg
     4981 agcactctca aaaaaagatc taccacttga ctgatgaagg acgtgctctt gtagaggaat
     5041 ggatcaaaga ggaaactccc gctcctacgc aaaaagatga atttctagca aaaatctatg
     5101 ctttttcgac gctcgataaa acaacagcta ctggactgct attcactaga gaacaacagc
     5161 tgaacaaaat catgcaaaaa aatcaaaaaa aattgaatga cctctcgcta gacaagaaac
     5221 aagaatttgg acgttatgtt gtgatccagc gcagaattat gctttgtcag caggaactcc
     5281 aatggtgtca gcaagtaaga gaacaaatgc agcagttttt tgagtaaggg ataaacagaa
     5341 cctttctaga aatatctatt gaagagcaaa ttatcttcaa taaaagagca atacagttgg
     5401 aaaagaaaaa gcgatccgaa cgacaggcca aaacctatcg ttcggatcac tatttttcta
     5461 gaactgcatt caagagacta tgacaaaaag catgccaagg ccttttttca aataacagtg
     5521 ttccatcatt cattactttg aattgctttt agcacacttc ttgaaagcgg gccaacgatg
     5581 atcaactgca acggtaaagc ggcaatcaca ttcattttcc aagcattcat ataagcagag
     5641 aataattgat caggtattcc ttgttccatg atcacaccga ataaagacat acatgtcacc
     5701 attcctaaaa ccatcaagca tgaaatagtt aagatcattt ggaccttgct ttctggattt
     5761 actggtagtt taaaggcaat ttttttcgcc agcacaccga tgatgaagac atccaaaatg
     5821 aaagccacca cgaatccagg cactagacca atcaacaaac ttgataaatg aagatcttga
     5881 tgcagccata aattatacgc gctcatcccc aagaccatca aaaaacacat cattgttgta
     5941 aaaattattc cttctttttt atttgtaggc aatctgtacc actcctttaa atttgtcacg
     6001 aaaatcatcg tagcacattt aaaaatttct cgtaagtact ttttttattt tctgcccttt
     6061 tatttcataa acttcatcag ccgtacgctc gacaaagtaa gaatcatgag aagtaaacaa
     6121 aatcgttccc ggatatattt gcattagctt ctctaaagct tcgatcgttg ccatatcaat
     6181 aaaattagtc ggttcatcta acagcaacac attacttggc ttagtaaaaa gcaaagcaat
     6241 cgtcagacgg gtcgcttctc ccccacttaa tgtacataac ggtttcgtca aagtttcctg
     6301 agcaaaccct aagtgattca gcaaactacg gacaagcgat tctgaaaact ctgtttgtct
     6361 catgacaaaa cgaatgatgg attcttcaga tgtcatttga taaccctttt gctgatatac
     6421 ttgaaagctt actttcggag agagaaggat tccttctcct tgtttgcgga tatgttctaa
     6481 taaggttgtc ttacctgaac cattttcacc gacaatcgcg atttttttac ctaaagaaaa
     6541 ctgaaaatca ccatttacca gtaatgttct cccacttctt tccaattgga cattttgtcc
     6601 catgatggga tagcggctgt ggagttcaag agattttggt actggaaaac ggatttgttt
     6661 catctgctgc ggcttggttg tttctcctat tttttggagt cttctctcta atgttttcgc
     6721 attcttttgg atggcttttt gtacactgtc tttttgttta gaggaagaca accgatccgg
     6781 tctgatttgt tgctttttct ttttactgga cactttttcc aacttttccg cttgtttttt
     6841 cttttcttgg atcgattttt ccaatcgttt tttctctttc atgatcgact ctgcttctcg
     6901 tagctgagtc tttttctcca attccttttg agtgagatag gcactataat tccccgaaaa
     6961 ctctcggatt tctccgtctt ttacttccca gattttcgat gctagctcat ctagaaaata
     7021 gcgatcatga ctaacaaaaa ccagcgtacc ataataataa cgaagctctt caatcagtcg
     7081 atcaatactt tttctatcta gatgggttgt tggctcgtct agaagcatcc cttgttcata
     7141 agaagaaaga agacgtatca actcgatctt tcgttcttcc cctccgctcc atttccgctc
     7201 tgaagtttct tccagtctta actggaaatg actggctaat tccaaattta ggtcctttgc
     7261 ttcagcgaga taggttaatt gaggcaggta gttgaaggtg atctctgttt ggattttccc
     7321 atgatcggga aaaagttcac cggctattag tttgagcaaa gtcgatttac cgacaccatt
     7381 ttctcctatg atccctatac gatcttgctg atagacacga agagaatcaa tagatagttc
     7441 tagctggttg ccaaagctga cttgtagatt tgttatattt actgctaaat tttccatatg
     7501 ttttacactc ctatttcatg taaaacaaca attatcgata ctaaaaaaga gcagaaaaat
     7561 ctgtttgcaa aaaaactgca tcacatcttt tcctactctg aacgaaataa ctatgactaa
     7621 atcaccaaat ctgtcatagc tttcacctat tctactgtcg ttcgaaaaaa tggacatact
     7681 ccatccatga aattgttgtt tatacatgtc ttttgaattt atttagttgt tattcaaaag
     7741 ttcgaaacgt aatttcatcg atgcagtcat ttttccgacc tcctttttta ttaggttacc
     7801 acacacgatt caaaaaaagc aatagctact gaatagtaac tgaaaacctt ttattcgaac
     7861 gtagtgatct tttctggtgc taaacgaaca gactcgtaac cttcttctac cgcttttcct
     7921 attgataaaa taataactgg aacataacgg tctttatcca aaccaaatgc ctctgccaac
     7981 tgatctgctt cgaatccacc aattggattt gtttcatagc catgatcacg cgcaactaac
     8041 atgaattgca tagccgctaa gctagaatcg actttgacta catcgttcat ttcttctctt
     8101 gaaaaactct tgtaataagg aatgatcgca gctaattgtt gatctctgac ttcttttggc
     8161 atcttccctg attcgtaagc ttggttatag atttcttcgc catattcgta acattgcata
     8221 tcgccaaaaa tcatgaccat tgcagaagaa gtatcatttt gtctcgtatt gaaacggatc
     8281 aaaggacgca agatttcttt tgcttcctcg ctttcagcga ccacaaagcg ccatggttgc
     8341 atattgacag aagaaggtgc agtcgtcgct tctttgatca tttccagcat ttcttcgtgt
     8401 gggattttgt atgtttcatc atatacgcga acggatcttc taccagtcat aattgttgaa
     8461 aaatcatttt tttgttccat tattattaac ctctttcgtt tctgttgtta tattgcaaag
     8521 ttgggtattc aatttatcta aaagtgttag aaattgtttc atttctacat cgctgaaagc
     8581 cggggatagc acggaacctg tatctttatg gtccatttca caatgctcga ttttcttctg
     8641 ggctttttct gttagcgcta catacacttc ccggttgttc tgaggatttc tctgacgcga
     8701 aacatagcca tttgcttcca ataccttcag atgacgagta actgctcctt gatcaatctg
     8761 caacttttct tgcaaactgc tttgtagcaa tggattttct tctttcagtc tcatcatgat
     8821 ctcgtatcta gtaagactta taccgatatt ttcttcaaag atctggacga ttttttgatc
     8881 ggctaatttg atttgatata aaaggtgact aatctcttct aaagacataa tttcctcctt
     8941 tctaataatt gactcgtcaa cgattgactt gtcaataata gaataaaaaa agaaaaaagt
     9001 caaagaatca gctgtgtaat aaaaaaaacg ctctcaatag aaaagtatag agaaaagaaa
     9061 ttttttgtag aatgaacgga aaaaatgata aagaaagcac aacgctaaaa ccaagattat
     9121 tgattcttat caaactatag aagatagctc ttctgcagta gaaaatttta caccgccaaa
     9181 aatttccgaa atcgcctttt gcaagaatcc cgtcgaatat tagaaattgg atcagtatgc
     9241 ttaaacagta ataaaaaagt tatgacaaga tgttgtcaca accttttttg agtaaacggt
     9301 gttcaaaaac ttgcatctgc ggtaataagt tcaaccaaac aaaaaacatg aaaatctgtt
     9361 tttgtttggc tgttcttact acttgatgca gggcagcttt ttcactacct ctttttaata
     9421 accataaaat cctattcctt catatctcca gcccatcggt gttagccaat ctctttcctt
     9481 agcactcaag gtatagtgat gggtaccagt tttttcattt ggattaaata gtcgataaat
     9541 tggcttggaa ccaccagaat aaaaacttag accttcataa cgccaaccga tttttgttaa
     9601 agagttgatc tcagattgtg ccattgtgta atgatggtct ccactgtaag gattgtataa
     9661 acggtagaca ggttgtccgc tgtttggtgc ttgccaagca atcccttcat attcccagcc
     9721 tacagttggc agataatttt tttctccggc attcaatgta tataaatgtt ctcctgtatg
     9781 acggttatac agacgataca tcggagaagt tgaagttggt gctgtatcat actgtgtcag
     9841 attataggtt tcgattacat tgttcaatgt agaaccataa ttaggcgcag tggcataacg
     9901 acctgttagc caagccgtag catctttgta agagttcgta ttgcttttcc atgcaccgga
     9961 atagtagtaa acgcctggct gaaaagaagt cgtcttcaat accgttacgt tatcttgcaa
    10021 tgattctttg tacgaaggat attttctaaa agcggcattc accgtaatcc attgcccatt
    10081 cacatattcc gaagtaggca tcgtaacaga ttggccatta tagcttcctt taatcccaaa
    10141 tagattgtaa ttaggggcag aagccaaagt actgttcccc cagccacttt caacgatggc
    10201 ttgagcaatc atgactgatg catataagtc attggcactt gccaaacttt gtgcagttga
    10261 ggcaatacta tcaatgaacg cttgttgaga attagcagat gagcgagcat aaaaagcata
    10321 gccagtatag ttttcgaatt cagacaaatc tgcttgatgg gtatgtgtca atgtttgccc
    10381 atctgtcaat tggttcgtga tttcttctgc attctctgga ttgttctgag cttctgctga
    10441 tgtactgtct tcggtttgtg tttgttctgt tgtttcagtt gttggttctt cggttgaaat
    10501 tgtattttct gagacagtag ctgactgcaa agtactctct gtatcgtttt caattcctgt
    10561 ttctgaagaa ctcgatgtct gagttgcgac agtttctgtc gattcttcag ctagcactgt
    10621 tgtgttggca actgctagat ttcctaaaag aataacgcta aataataaat tctttgcttt
    10681 catctttttt ctcctttgaa ttatgaaact tttcctaaat aacagtgtaa ctaaaaaagt
    10741 aaaagcaagt caatgtccat atttaaataa catttgtttc tttttgataa atttgctttt
    10801 tctacataaa aagacgtaaa tgaagaatat tcttctccat tcacgcctta aattgatggt
    10861 cgtttgaata actatttaac aagtaattct ctttggatat tatccgttat ggattatact
    10921 tcctgtgtcg tcggacggat aatcatgtta ctgacagaca tccggtctgg cgtttctatc
    10981 gcaaatgcta cagcagaagc tatatcttcg gaggtcaatc cccattcttt ttgcgcttca
    11041 tgaagttcaa gagcagcctt tttgtcgctg atggtttgat ataattcagt ttgaacagct
    11101 cctggagaga tgatcgtaga tttgatgtta ttttgccgtt gctcttgacg taatccttcc
    11161 atgattgcac ggacagcaaa ttttgttccg caataaacgg cggaatcagg ataaacaaca
    11221 tgtcccgcca cagaatcagt tgaaatgata tgcccagatt tttgtttttc catgattgga
    11281 agtacggcgg aaattccatt taacacaccc atgatgttga tatcaagcat attttgccat
    11341 tcatcacggt gaccttcaac taatggagcg gttggcatga ttcctgcatt attgtacaat
    11401 acatcgattc gaccgaactt ttccatcgct aaatcgataa ctgcttggac ttgtgcaaaa
    11461 tctcttacat ctgctgtttg aatatatagc tcagcttctg gtaaagattc ttttattgct
    11521 tttaggcgat cttctcggcg tgcagctata actagtttag cccctttttc tgctaatagc
    11581 ttcgttgttg cttcaccgat tccgcttgaa gcgcccatga tgacaatcac tttttctgat
    11641 aaatcattca ttctattctc ctcctgtttt gaaaaatctc ttgcttatac tcactatctc
    11701 ttatttcatc ttactgttca aataatccgt tcgacaggtt tgacgatttt ggcagggatt
    11761 cctgcaacga tggtattgtc tgggacatct ttcgtaacag ttgcattcgc tgcaacaatg
    11821 gcattctcgc caatcgttac gcctggcaga atagttgctc ctgctccgat ccaagcattt
    11881 ttcttgatgt ggattggctt tactgacaag cctcttcgtt cttttggatc ggttagatgg
    11941 ttcaccgtga tgaggttgac acgaggacca atcaatactg aatcttctaa acagatgcca
    12001 cctaagtcaa cgaacatcac ttgacgattg ataaagacat ttttccctat actaatatgt
    12061 ttgccgaaat ctgtataaaa aggcagcaga acagtcaccg tatcatctac tttgtcacta
    12121 atgatatttt ctagccgttt gcggatatct gctggttcat ggtacccagt attcagcgct
    12181 gctaaaggct cttcgttttc agcttggatc tgatggatca cttcaaagag cggatccgtt
    12241 actcgaatct ctttattgac gatttgtttt tcgatgaggc ttgtcatttt gctccctcct
    12301 tttatcctta ctttataacg gacggcttat catgtctaat acttataaaa catacttaac
    12361 tattcctttt aactatactt agtggtatgc ttttttaaaa acaggaggga aaaaatggat
    12421 attcgtatac tgacgtattt tttagcagta gctagagaaa aaaatatcag ccgagcagca
    12481 aaatctctgc atatcacgca acctactctc tcaaaacaac tgaaagattt agaagaagaa
    12541 cttggtgtga cacttttcac tcggggctcc cgagagatac agttgacgga ttctggtcga
    12601 tatctttatt caaaaggaaa agaaatcttg tcgttggtag aaaaaacggc gaacaattta
    12661 acaaatgacg caatcatctc tggagaaatc tacattggcg gaggcgaaac taaagcaatc
    12721 tctttcattt ctgatagcct tcattcgttg atcaaggcgc atcctgatat ccagtttcat
    12781 ttatatagcg ggaatgcaga tgatatcata gacaaactgg atagcggatt acttgatttt
    12841 ggactcgtaa tcgaaccgac aaacaaacaa aaataccagt catttcgact gccagcccaa
    12901 gatcgttggg gacttttgat acatcgaaat catcctttag caaagcaaac cgtcatccat
    12961 ccgcaggaac tgaaaactgt tcctttgatc atttcccggc aaacgtcagt ggacagtcaa
    13021 ctagctgaat ggctaggttt cagcatagat cagttatcga tcgtcggaac atataactta
    13081 ttgtacaatg cttctttgat ggtcgataac gggacttttg gtgcattagc tctagatggg
    13141 atcatcaata cttatgggac ggaaaaaata ttcatcccac tcgatccgcc attacaagct
    13201 tcactgaatc tcatttggaa aaaagaccag cctctttctc ctgctgctcg taaattttta
    13261 gatgtcctaa cggagaaata cagttaagta gagttttttt ccttcgcttt cagcttttta
    13321 acaacgaata agataagcaa aagatccaac gattaaataa cataagtttc ttaatttatt
    13381 tcttcctttt atttctgata attccagtaa attactataa ttataaaaaa agaattgaca
    13441 aaacataact gtctatataa aattgattac gttaacaata attcgtttca tgtgactaga
    13501 tcatactagg catgaaaaat ctctgaggaa catggtttcc ctaggaggtt tttcgtgtct
    13561 atttttgttt ggccgcaatc aactggtcta gcaaacgtga aaaccaaatc ttcaaaaagg
    13621 gtgcgcgcat gaaaatcaaa aagatcttta atcaaaatgc agtattggta gatgacggtg
    13681 gacaggaaaa agtagctatt ggaaaaggga ttggatttga cagaaaaaga aatgatttga
    13741 tttttgatcg tgatgtcgaa cggatatttg tcatggaacc agaaggtcaa atcaagttgc
    13801 aaaatttact taatcagatt gatgaacgct ttttatttgc agcagaaacg atcattgatc
    13861 atgcggaaac tgttttgatg gaaaagttga atgaacatgt attgatcgct ttgaccgacc
    13921 acattgcttt ttctgcggag aacattaata acggtatcgt catccgcaac aaacttttaa
    13981 gagaaatcga agtgctttac agtgatgaat tcagtattgc tcaatgggca gtagagtatc
    14041 tgacaaaaga gcttgggatt ccatatactt atgacgaagc aggatatatc gcaatccata
    14101 ttcacagcgg acgaagcggt cggaacaata accatcgcag tatccgtgaa gtgacgattg
    14161 tatctgacat catccattta gtggaatcag aattggaaac agatatccat tcagaaccat
    14221 tttcactgaa ttattcaaga ctggtgaatc atttacgact gcttctacaa cgaacccatg
    14281 cgcaacaata tgctgtttta gacacggaaa tcgtagaaat ggtcaaacga aaatacccag
    14341 aaagctataa gatttctaaa aaaatccgtg tgttgctgac caaagaatac cagctcgcga
    14401 tcacgaaaga agaactgggt tacttggcca tccatatcga gcgtttaaga agcgcaatcg
    14461 tacaaacaaa tgttaacaat catgaggagg aaaaaaattg aaagcatatc tacaacgaat
    14521 gggacgttcc cttcaacttc cagttgcggt cttacctgct gctgctttac tagtagggat
    14581 cggaaactgg tgggcatctt atagtaacga cattgtcgct cattttttac aagctggcgg
    14641 taacgcagtt ttaggtcaat tacctttgct gtttgccgtt gggttagcac tagggatgtc
    14701 agtggacaaa tcaggtgctg ctgccttggc aggtttggtc gcatttgaag ttccagtaaa
    14761 tgttttaaaa actgattcag tagctacttt attgaatatc aaaccagaat cagttgatcc
    14821 atcttttgct cagatcagta acgtatttat tggtattatt gccggattga ttgcggcagc
    14881 tctttataat cgtttccacg aagcaaaatt accgatggct ttatctttct ttagcggcaa
    14941 gcgtctagtg ccaatcttgg ctgcctttgt catgttgctg atcagtgcag tgctactgct
    15001 cgtatggcct ccggtttact ctgctttagt ttcttttgct aaattcattt tgagtttagg
    15061 ctggggtggt gcaggtcttt atggcttctt caaccgttta ctgatcccta caggattgca
    15121 tcaagcaatg aactccgtgt tctggtttga cgtagcagga atcaatgata ttggtaaatt
    15181 tttggcttct gaaggaacaa aaggcgtaac aggaatgtat caagctggat ttttcccaat
    15241 catgatgttc gggcttccag caggagcata tgctatttac agaaatgcac gtccagaaaa
    15301 caaagcaaaa acagcttcct tgatgctagc agcagctttt gcttcattct ttactggtgt
    15361 gacagaacca cttgaatttt catttatgtt cgttgcttgg ccattatatg tcttgcatgc
    15421 aattttcact gggatttctt tagcagtgag tgcattcttc cattggacag caggatttgc
    15481 ctttagtgcc ggatttgtcg attacttcct atctttgaaa aatccgattg cgaatcagcc
    15541 attgatgttg atcgtccaag gattagtaac agcagtgatt tactactttg gctttaactt
    15601 tgctatcaaa aaattccatc tgatgactcc tggccgcgag gaagcagatc tttcagatga
    15661 cgatactgca acgactaatt cagacaataa atatgcggca caagcaagca aaatctacgc
    15721 tgcattaggc ggagccgaca atgtaacttc gatcgataac tgtactacac gccttcgttt
    15781 gcaagtcaaa gatacaggcc tgatcgatca aaataaaatc aaagccactg gcgtacctgg
    15841 tatgaagatc atcgacggaa aaaatgcgca agtcatcgta ggtacagaag tacaattcgt
    15901 tgcagatgag atggcaaaac tacatggtgg tgctgcagca cgacctgcac aaacaaacac
    15961 cgtggttaaa acagaaacat ttgccggtga aacgatcgaa caagatattt acgctatcgc
    16021 aaatgggaaa ctgattccaa tcactgaagt ttcagatgat gtattcgcgg aaaaaatgat
    16081 gggtgacggc tatgctgtat taccagaaaa tggagaaatc ttctctccaa tcgctggtac
    16141 gatcacaaac attttcccaa cgaaacatgc tgtcggaatc caaacagatg ctggtatcga
    16201 agtcttgctt catatgggca tcaacactgt tgacttaaaa ggcgaaccat tcactttata
    16261 tgtagaagaa ggtcaaaaag tcgcaagagg tcaattgatt gctttagttg atcttgccgc
    16321 tattcaatct gcaggcaaaa acacggatat ggtcgttgta ttcacaaatg gcgacaaagt
    16381 ccaaagctta gaaatcgaac ccgcacgtga tgtaaaagca aatgataaaa tcggctctgt
    16441 ttccaacaaa gcctgagcca tcgtcttccc cagacttttt tatcgataaa caaacaggag
    16501 ctgagacaaa aatcttgtct cagctccttt ttccgaataa aaacggcgtt ctcctgccaa
    16561 ctccgcggta ataagtccag ctaaacgaaa catatgaaga gctatttttt atttgactct
    16621 tcttattact tgatgcagaa cagctttttc acaaaatttt tcttcatact caaaactaat
    16681 cggctttttc acaacctctt tatacattat tcctgatcca atatttcttt taatgctgct
    16741 tcaagagaag ttaatggttt tcccaatact ttttctaaat catcagagga aatagctaac
    16801 tgatcctttt taatatcatg ttggatcgct aaaaacatgt cagctgcttc ttgagggaat
    16861 ccggcttctt ttagattttc gacaaacgct gcatcacttg cttcaataat ttctaactct
    16921 ttacctgtcg cacgctctaa tgccttggct aattcttcat agtcagttag attgccggat
    16981 aattctaaga tttctggaga gtcactttct actagtactt ttgctgcagc ttctgcatat
    17041 tcacgtttca atgcccaacc tgtttttcca tttcctgcag cataaacgaa ttgaccagtt
    17101 tttaaagcct gcccaatgat aggtaattca ttctctacat accaattatt ccgtaaaaat
    17161 gtatgggaaa tacctgaatc cttaatcatt ttttcagtga attggtgatc tggcgctaat
    17221 acactgatcg agtgatcggc atctgcaaaa cttgtataag cgatataaga taccccagct
    17281 gcttgtgctg ccttcactac attcttatgt tcggcttgac gatttcctgg aacgccagag
    17341 acaaaaagca atcgatctat tcctttcaac gcttctttca ttgagtctaa atcatcataa
    17401 tcaccaatac gtatattaaa tccagcttct ttcagtgctt ctcctttttc ttcactacgt
    17461 actagtacat agatttcatt ttgtggaacc atctttttta agaattccag cgcataacca
    17521 ccaaaacctc cagttgctcc tgttactaaa taatgcatat ttgccactcc ctattctttt
    17581 tatttgttaa gatttacagc tgtacattta taaattacac ctttatcata aagagtgtgg
    17641 taagataagt caagcaaaaa aaatgaaagg atgacaactg atgaagtatt ctctgcaatt
    17701 tagtgatgca atccatgtac ttgcctatat tgaaatgttt caagataccc gatcactttc
    17761 tagtgagatg attgccagta gtatcgaaac aaatgcagca aatgtacgta aaatcatgag
    17821 tcagctcaaa aaatccggtt tgatctttac aaaaaccgga aaaccgcagc cttcccttgc
    17881 caaaagtcca gatcagattt ctttactaga tgtctatcgc agcatcgaag gtaatacaca
    17941 gcttatccaa gtcgatccaa aaacaaatcc aaattgcgtc gttggtgcga atatccaaga
    18001 agctttagaa gaaagctacg ctcggctgca gaaaaaggca gaagaagaaa tggctaagat
    18061 cacacttgat agtctgatac ataaaatagc cgttttggaa aaagaaaaaa gaccagaaaa
    18121 cacaggcctc gtagagagat ttttataatt ctctctactg tgcctgcatt tttctgatcg
    18181 gaatcgtcta cataaattct tttattctcc gggggacaaa agccttgtca tgcccctttt
    18241 tcctctctaa tgctcaggtc aaagcgctga agtcatgacg tcttgataaa cggtgttcaa
    18301 gtagctgacc ctcggcatta gttctgaaaa atgcaaaata tggaaagcta tttcccattt
    18361 ttcctctcta atgctcaggt caaagcgcta ctttcacgac ctctatatta tcatggttta
    18421 ttgactaccg gtatccagat ttctcccata tatttcccgt tggtaactcc catttcgact
    18481 acagcatttg gacccccaac ataggcgtaa tcttctactt ctcttaaaac atgcccaaag
    18541 gccgtattcg tcaaatcatt tcttaattct tcaggcgatt ctgctaaagc gcttacaaca
    18601 agatacgttc cttttgggaa tgggatgatt cgcgtagtgt ttggaacatc ctgactcgtt
    18661 tgaacaccga catagtacat cattttcccg ttatagggtt cgttgactgt aaagaaataa
    18721 tgattttcag cagcttgttt caatgcatct attgttccat taccgataac tttttgctca
    18781 aatgcttgtt tctctgcagc caaaccgaca aaatccgtat tgctactctt caattccaca
    18841 ccaatacccg tcaccgtaaa atctggtttt tcttcgatcc tatactttgc catttctccc
    18901 accctttcta ttatattaag ttcatcttac ataaaattaa agggaaatga cacgattacg
    18961 ctcttttttc tagggatatt tttatttttt aactattttt ttgctttcac tgcaatccaa
    19021 atctcactgt aaacattact gaaatcctct ccccattttg taaaagagat ctcgggagct
    19081 tcgactaacc cataatctga agaaggaagc cattctgcat agattttccc ccaagtatct
    19141 tgtaaagttt ggggaaatgg ccctttatta ggaaaaatgg cccaagtgtg ttcttctata
    19201 gcaagcattt ctaaatcagc gtacggattt tcttttgtcg tagcaaagcc aatcatgtgc
    19261 atcaaactgc ccttttcttc caagcggtct ccatcaaatc cataagaaac gttgattact
    19321 tgatttggat ataaatctcc taacttatgc atctcttccc tttgagtctc cgtgatcgtc
    19381 tgcgctaact tttgtatttc ttgattgact ccttcaaatt tgacaggaac ttttttcgcc
    19441 acacctgcca cataaaactg ttctttcttt tcgatacgaa attccatcga ttttcctcct
    19501 ctgacatcaa taaaaaatgt caagcgtgga aaagattttt gtatgccgta tctactgatt
    19561 tctgaaggca gatatcctga ccaatcacga aaagctcttg aaaaaccttc gactgtttca
    19621 tatccatatc taaacgccgt ttctgtcact cttgatccat ctagaagatc gcgattagcc
    19681 aaagccaatc tacggttttt gatatagtcc attagcgaca tccctgcaat aaacgtaaag
    19741 atgcgtttta attgatattc agagattcct gtattttcgg ctattatttt ataggaaagt
    19801 ccttctgtca aatgttcttc gatgtaatcg atcaattggt tcagatcttt aagcatattt
    19861 atcttcctcc ttgattgcct ttttatcata ttatcaaaga aagtcaatta cttgatgaat
    19921 caggaaccaa tttagtcaga tgtagaaaat gttgtatgga tctaaagaaa attctcaagc
    19981 agcgtttcgt aacggataat tggtattatc tgcctatcta tgttttacct ttagaaaaat
    20041 aaattttggg gaagacagat tctaagtaaa caaagcggta aaaatatctt ttattccctt
    20101 agttaatccg cttactacct actgtacaaa gcaaattgtt gttcaaactg ctgatcagaa
    20161 cgctgcttgg atttttcttt gcgatacatc atacctcttt tatcaattgg aacaattatt
    20221 tatttctaac aaaatcactt catttttgtc agataaataa aaaacaaaaa aagttccatc
    20281 tttaaaacac gctattcttt tactttttct cacattctct aagaaaagaa aaggagaatg
    20341 aatttttgac aattcgagca aaagtgatta tcaaatatgt aaaatcagct acttttttct
    20401 taaacgtttc tttatactaa atataaataa gtgtttcatg aaaggagaac tagatgaata
    20461 attggcttga gtattttcct gaaaacgttt tggaacgcgg ttacagctat catctgcacg
    20521 gttttgtccg tcatttgaac tatacaagca agtacttgtc cgcaacagtt tctggcacgg
    20581 aagattataa ggtagtgatc acttgggatg aaaagaccaa catgacttgt gattgtctat
    20641 atgccataga aggaaaaaaa tgcaaacaca tggctgctgt cttattcgct tacgaagaac
    20701 gtccaataaa aaaatccaac tactcacttt ccgaactctc gtcccttgtt tcctccgcta
    20761 gttcctctct tgtacgggag cttttaacag agattcttat agaacatccg cactttatcg
    20821 aacgcttcaa agttaagatg ccatttcatg caataaatta ttcagataaa ttaacaacta
    20881 tcatccataa atacgatcat attatcaaga aaaacaaaaa caggaaaaca gcgaagttca
    20941 tcatggagat gcgaaagttc atccaagaag cagttgaatc gcttattcag caaaatgcct
    21001 atcttcccgc attcgaattg atcaatgaag tgattgctac tctcgaaaca ttctattggg
    21061 agcctgagga tgaacgaacc cttcttctga tagaggactg ttattattta tggaaagaat
    21121 tattagcaga agcacctcat gcagaaaaaa ggcaaatgtt ctcttggttc gtctgccaag
    21181 tcgatcatac tgacgcctct tattcaaaac gctactctat aaaaatcctg aaagaagact
    21241 ttcgggaaaa agaattttct aaccaaaaga aaaaaataga taaaaaacta aagaaacggt
    21301 aaagaaagac gatttcaatg aaaaatagca agtaaacgat aacaaccatc ctaaatccac
    21361 aagaaataca gcagaccttg ctttaaaaac acgaatcatg caaaggatga agtaaagaag
    21421 acttaacgcg tacccaatat tagtcatttg actattaggt acgcgttttg ttttataagt
    21481 catgacaaat ttccttcgtt gctggataga ttttccggca ttgttgatag attttatgat
    21541 acttttctac attttgtaag agaggttccg ctgcatctgt atattggata aaattctgac
    21601 aacaactttc aggtgtgtcg aaccacccta accctagtgc agcaatcatt gttgcgccta
    21661 atgcaggtcc ttcctccata cttaaacaat agaccgttgc atcgaaaata tcagcttgga
    21721 tttgtagcca tgcaagattt ttcgcgcctc ctcccaccga aacaatacgt gtaaatctgc
    21781 gacccgctag ttctttacgt ttcaatccca ccattgacaa atgctcatta aaaatttaga
    21841 atattcacaa gtttaacgac aattatgaaa atttatgtcg tgctaaagga gttttgttat
    21901 tggatgaaaa gttagatata aaaactgcga tcaaagacac gttacctacc gttttcggtt
    21961 atatcggtat tggacttgca tttggtatcg ttgggaaagc tgccggattt catccactag
    22021 tcgtcacgtt gatgtccctg ctggtctatg ctggttctgc ccaatttatc acagtcagca
    22081 tgcttgctag tcacagccca ttgctttcca tcgttttctc gacctttcta gtcaattccc
    22141 gaatgattct gatgagtatg acgattgctc cttatttcaa gaaaaatcgg ctgcttcaaa
    22201 atctgctgat tggcacattg ctgacagatg aaagtttcgc actgggaatg aataaattga
    22261 actatactgg acaaaagtta aactttcgtt ggatgaatac agctaattgg atttcttatt
    22321 tgacgtgggt gggttcttct cttgttggcg cacttttagg gaattttatt actgatccga
    22381 aaaagtttgg tttggatttc gccatcgtcg ctatgtttat cgggttactt tatcttcaag
    22441 tcatttctga tagaaacacc tctaaacgat tacagttgat cctgattggt ctcactttga
    22501 ttctggtata tgtcggactg atttttatcc caagcaactt agtgattgta gttgtaacac
    22561 tgattgggtg cggattgggg gtgtggatca aacatgcctt cttttgagtt tacattgctg
    22621 actattttag gttgtacttt agcaacttgg ctttctaggg tcctaccatt tgtcttactg
    22681 aaaaaattcg atttaccgca accgttgctt gagtatttaa gttttgtccc gatcgtcatt
    22741 atgtctgctt tatggttcag cagtctattt acccaaaata tcggacatct gccgcagatc
    22801 aatctcgaaa atgctttggc ttcagttcct acacttttag ctgctattct ttcaaaaagc
    22861 ttattggtga tcgtcttggc aggaattctt tccctttcgt tgattcgctt attataaata
    22921 aaaaaacaag agcttatggc aaattactgt catgcgctct tgtttttatc aaagtcaaat
    22981 aagaaaaaaa tcggcgtatt cttgcttatc cgttgaacgg accactaaca acgacttttc
    23041 ctaacatatg tcctgtttct acgtctttgg ttgcttgttt caagttcttc gcattcatcc
    23101 catctgaata gaccttgctc aatgtactgc ggataatccc ttcatttgct aaagcagcaa
    23161 tgtgcgctaa agcttctcct tgcgtttcga tttcatagtt atagtctgtt ttagcgaaca
    23221 tatactccca gtcgaagctg atagattggt ttttccaaac acctatatct aacggctctt
    23281 tgatccctac gatcgttccc acatgtccaa atggtcggac aaggtgcttg atttgatcaa
    23341 agtagcgggt aatatcgaat aatacagcga catactctac ttgatcgatt cctaattcac
    23401 gaaaactttt acttaagtcg tgatgataat cgattggata gtctacacct gtttttttca
    23461 gccattcaaa attcttcgga cttgcagtcg cgtagacttc taggccagcc caatgcgcta
    23521 gttgattcaa aatcgaaccg acgcccccgg atccgttgat cactaagatt tttttcccgc
    23581 gattggcatt ttcttctgga gttagaccaa atttttcaaa taacaattca taagcggtca
    23641 atgaggtcaa cggcaaggct gcagcttctt catctgaaag attttttggt gcaagagcga
    23701 caatacgttc gtcaactagt tggtattctt gattgcttcc tgctcttgtt gttgtacctg
    23761 cgtaaaaaac gcgatcgccg acagaaaatt tttgcacttc gtcacctaca gcaacaactt
    23821 tcccgactcc atcaaaacct aagatacgta aagcatttct tatcccgtta tcttggcgaa
    23881 gtttcgtatc gacaggatta acagatactg cgttgacttt taccagaaga tcccgtgaac
    23941 tgggtacagg tatctgttct ttttcatcaa taaagctttt tggatcatcg atcggcaacc
    24001 cttcataaaa accgactacc ttcattaatt ccttttctga catacgaatt cctccttagc
    24061 ttcatcaagt tggagcatcc atacagtgtt catttcaata tatatagtat gctatttgtt
    24121 ccaccttttt tattcatcat aattattctc ttacttaaag taaactattt gcactttttt
    24181 ggttaaaaaa gaggttgcga caagactttt gtcacaacct cttataaacg gtgttctcct
    24241 accaactctt cggtcacaag tcaaaatatt catcaaacat gagaagctgt tttttgaaat
    24301 tgctccttgt gactcagagt taaacggcag gctcacaacc tctgatataa acggtgttca
    24361 atcaactgac cctcggtatt agctcaaaaa acgacaaaat atgaagagct atttttgttt
    24421 ggctgttctt attacttgat acaggacagc tttttcacga cctctaattc acggtgttca
    24481 agcaagaaat cattgttcac tgaaaagcgt tggatgttct ttttcaaagg tctctaagat
    24541 ctctcgtact tcatcagtag atttcgtatt catcaactga actcgcaagt catttgctcc
    24601 tggaaatcct ttgatataga ttttaaagaa acgatgtagt cctgtgatgg aacgcgggat
    24661 gatttctgaa taatgatctt gcagatctag ttgcagacga agtagtccga tcagttcttt
    24721 ttctgtatgt tccctcgggt ctttttcaaa tgcataagga ttcttgaaaa tccctcggcc
    24781 aatcatgatg ccatcaacgc catatttctc ggccaattct tctcctgtct ggcgatcggg
    24841 gatatctcca ttgatcgtga ttagtgtttg tggagcgatt tggtcacgta aagcaatgat
    24901 tttaggaatg acttcccaat gtgcatctac tttactcatc tctttacgcg tacgcaaatg
    24961 gacagaaaga ttggctatat cttgatgaag taggtgactg atccattctt ccatctcaga
    25021 catttccgta tagccgatac gcgtcttgac gctgacaggc aaaccgccag ctttagctgc
    25081 ttcaatcaat tgtgccgcta cttctggacg aaggatcaag ccactgcctt ttccacgttc
    25141 tgctacatta gggactgggc agcccatgtt gatatcgatt ccttggaagc ccatttctgc
    25201 catagcaatg ctcatttctc taaaaaattc tggcttgtct ccccaaatat gggcaaccat
    25261 cggctgttca tcttctgtaa atgttaatct tcctcgtaca ctgtcttttc catctggatg
    25321 acagaaactg tctgagtttg taaactcagt aaagaagaca tccggtgctg ctgcatgctt
    25381 tacgacatgg cgaaatacaa catctgttac atcttccatt ggtgctaaaa caaaaaaagg
    25441 ctttggcagc tctttccaaa aattagtagt catttatttg ttactctcca ttctatcaag
    25501 cagtgaagtc gcaatctttc tcaaaaacat tgtcattata ctaggaaaca aaaaaagaag
    25561 caaagtgaac agcttaaaag atgtttctga ttgagcaaaa aaaagactgt gacaaaaatc
    25621 ttatcacagt ccattttccg gaataaatgg tgttcaaaag cttgcatttg cggtaataag
    25681 tccaaccaaa cgaaaaatag gaggagctat ttttgtttgg ctgttcttat tacttgatgc
    25741 agaacagctt tttcacaacc tctccactaa acggtgttca atcagctgac cctcggtatt
    25801 cgctcaaaaa acaacaaaat atgaggaact attttcccgt ttgtttctct aatactcagg
    25861 tcaaaacgct gatttcacaa cctcttttct aacttctcaa tcctcttcct ttattgatca
    25921 aataccaagt aacggcgtac aaaacaacga tgaataacac aagtacgatc atcgatgttg
    25981 ctactggcac atcgatcgtt ccaaggaatc cgtaacggaa accggagatc atataaacga
    26041 tcggattgac ttttgaaaca gcttgccaaa agggcggtaa catagagatg gcatagaaaa
    26101 cgccacctag ataagttaac ggctgcaaga caaatgtagg cacgatcgat acatcatcat
    26161 aagactgagc aaaaatcccg ttgagcagtc ctgctaatga aaacaagatt gccgtcatca
    26221 ataatgtaat gatgaccatg aaccatgaat acacatgaag tggtacaaag aacaaggaaa
    26281 tgatcgttac gagagagccg acgagaatac tgcgacccac accaccaatc acaaagcccc
    26341 aaataatcac atgcgtagga acaggtgcta ccaagatttc ttcgatattt ttctgaaatt
    26401 tttgagagaa gaaggacgaa gaaacattgg catacgaact agtgatagca gacatcataa
    26461 tcaaaccagg aacgatgaat tccatatatg aaaaaccacc catgtcaccg atccgcccac
    26521 cgatcatttt tccgaaaata acaaaataca aggatgtcgt gatcactggc ggaaccagtg
    26581 tttggaccca tatacgcaaa tagcggttcg tttccttgat agctaaactt tttaatgcag
    26641 taaaataaag attaaacatt tttctcctcc gtatgattat tttcttctgt gattttcaag
    26701 aacagttctt ctaaacgatt cgacttattc ctcatagaaa gaactttgat tccttgtctt
    26761 gtaagctgat cgaagattcc gttcacccct tgatttcgtt caacttcgac agcgattgtc
    26821 tgctcatctt caaatgtaca agcatatcct tcaatcacag gtttggtatc atatggcgct
    26881 aaatcaaaga taaatgtttc aaattgcagt tttgacagaa gctctttcat acttgtattc
    26941 tcgatcagtt cacccgattg gatgattccg atgttgcgac aaagcatttc agcttcttct
    27001 agataatgcg tcgtcaaaat gatcgttgta ccattttcgt tcaattctcg tagaaactcc
    27061 cacatctcgc gtctcaactc gatgtcaact cctgctgttg gttcatccaa gatcagcaaa
    27121 cgtggttcat gcatcaatgc cctagcaatc atcagtcttc gcttcattcc cccggaaagc
    27181 atacgtgcac ggacatttct cttttcccaa agattggact gcttcaaata tttttcgctg
    27241 cgtttcatcg cttctttgcg agagacaccg tagtagcctg cttgattcac gacgatttgc
    27301 tggaccgttt caaaagggtt aaaattgaac tcttgtggaa ccaatccaat ctgttgcttt
    27361 gctcgaacta gatccttgtc cagatcataa ccgaagacat tcacttttcc agatgtttta
    27421 ttgactaaag aagtaataat cccgatcgtt gtagacttcc cagctccatt tggccctaat
    27481 agcgcataga aatctccttc tttcaccgaa agatcgattc cccgcaatgc ttcgacccca
    27541 cttccataga tctttttcaa attatttatt tcaagcgcat ttgccattta tttcctatct
    27601 ccttatagac atgattctta attgtatcat ggaatacagc agatgaaaaa acaacttggc
    27661 ttccacacaa aaacacacct gctaacaaag aggagatgtg ttttctagtt catgatcatc
    27721 ttcgatagaa taactatcta aacaactctg gaacttttaa aatcataaaa gtaccgacag
    27781 caactactac gatgacatat agaagatcct tataactgag gttcttgtct tttttattca
    27841 tgttaagctt tcctccgtcc ttttatcgaa agcttttttc ttttatttta accgaaataa
    27901 ataaaatgga aatagttatt ttcaatttct gttttcgctg tttgttttac ggagaacttt
    27961 gccatggaac gtgcgtctcg tcgctgatgc taagtttttt cgataaaagc tgctagaagg
    28021 aagacagcgt ggtttatttt gtatcgtctt ttgctaaatc ttccaagatc atctggacag
    28081 cagactcgtt cccattgaat tctgccactt ttttggcatc aatgtacttc ttttgataat
    28141 cgtctttttg gacccacgca tcatgcttcg tttgataaag ttaatcaaac ggataccact
    28201 gacgctgttc atcgaacaac aaactttctt tttgggcctg ttcaactatg gatttaggat
    28261 aattcaaaac tttcagcaag gcaatcgcat ttcgggtcct tgaaggtcct tcttttaatt
    28321 tgaaatcaaa actgattcct tttccttctg ttacttgttc ttcaaagtgg acattctcac
    28381 aataattctt taaaatctct gtcaattcga tatcatgtgt ggcgacaaat gctaaagatg
    28441 gatactccgc aagccaccga acgactgaag cggaagaagc aatccgttcg atcgtatttg
    28501 ttccttttaa aatctcatct acgaagcagt agcagcgttc tttggaagcc acttggtcca
    28561 gcaaacgttt gatcgatttg atttctgaaa caaaataact gtcgccttca aataaatcat
    28621 cttcgattgc catggaagtc aacacatgtc caggttgcag ggtaaactgt tctgcaatcg
    28681 ctgtttggat cgtctgtgct agaatacagc tgatggctat acttttgaca tacgtagatt
    28741 ttcctgatgc attggaaccc gtgactaatg tattcttctg ccagttgact ggattaacta
    28801 ccgcttcttt taacaatgga tgatagatat ctttcgctgt cacaccgccc tcagtaaact
    28861 ctggtcggca agtgatcggc atataggtgc gataattcaa aacggctgct gcaacttcca
    28921 attctcccag caattcccac agcttgatgg cttcagttga atgcttttct aatttagtga
    28981 gaacgaaatg ataggagata aatggcagca tgaacatgat gttgacatat tcaaaaatca
    29041 attctgcttc actcccgctc ttcattcgaa atgaaaaacc taatttaggg atgtctctta
    29101 atggtttagt ttgctgtttc agctcttttt gaagtggtgt cgtcattttt gctaactgat
    29161 tggcggctga aatcgttgaa accaaataac tcatgctatt cagctctgtt tctagtgttt
    29221 gtttcttcac tagataatag accgtattga atactaagcc accgattgct agatagattc
    29281 ctagtacttg ccctaaaaga agcaaaccta cacccaccag cggaagcaag ccaagaaaaa
    29341 taaataaacc tacgcgtcca atctttttac tttttccatc cgctagatat tgttttgcaa
    29401 aattatgatc tttttttcct aaacgagcga attgatattg gattctttct ctcagttgag
    29461 gattttcctg ataaaaagca attagttttt ctaactgata atcttctcca aaatcaaagc
    29521 tacgtaaacg ctgatacaac gcttctgaac ctacactaga ataggtactg ttgatccctt
    29581 caaatacttc gaacatatcc aagtcatacc aagtaagatc gtcgatttca ctatcccatg
    29641 aacggaattt tttctccgtg agccacgcat ccttcaaact ttcttcttta tcaaatctcg
    29701 gttgataagg aatttttccc caattactac gaactgtttc ctttactttc aaacgtgaaa
    29761 aaatgtctaa aacaactaat aaaataacaa ctgcaataat aattaaaacg ataaattctc
    29821 tttggcccat atccctctcc gttccttgat ttgttagttt tagtatagca ctgttgcgct
    29881 atttgaaaag ggggtacaaa aataactgtc ttcgttacct aattcgtatc aatcacacca
    29941 aaataaaaac accttccaaa aaagaagatg tctttaaaac gagatacaat tttttgtcat
    30001 tcacttcagg taaattagag ccaattaaac cctccgcaac ttgacggaga tggagaagat
    30061 gcccccgtct aatctagcgg tcatctctcc tcccattcct tccaccaatt tttttgacag
    30121 gtaaagcccg attccgctag attccgtatt tgtgcggctg gtattttcgg tataaaaacg
    30181 ctggaagact ttttcaacag aggtttgcgc tcctggtttg ataggattgg ccactgttaa
    30241 tcgaatgaat gaatcctctt ttgaaaatgt cacctttgct tgtccatctg cataacgcaa
    30301 taaattctgt atgatatttt gcagcactcg cagcaacatg agcggatctg ctaatacagt
    30361 tgtatttatc gagtgttcag cgaacgctac ctcgatattt ttctcagaaa attgttcata
    30421 aaaagagaaa aatacttctt ccacctcttt agaaaaagaa agcgtatgga gatccatttc
    30481 tttttgcttc gtctctattc gagcaagatc ataaaactga tccgtcatac tgagtaatct
    30541 tgcaacagat tgtttgatga tcgttaaata cttctcccgt ttctccgtat cctctacctg
    30601 ttccagtaat tgtacataac cgttaatagc agtcaaagga gttctgagat catgagaaag
    30661 gccgacgatc gatagtctga tgttttcttc catttcttga aatgcttgtc ctttttgttc
    30721 attttcatcc acccatgtat tcagtgcatc tactaggtcc attagattct tttctcgaaa
    30781 ttcgactgaa agacgaccac catattcttg tcgttcagaa agagtggcga ttttcttagt
    30841 taatctcttg atttcccttt gcaggaagaa aataaaactt cccataaaga taacaacgag
    30901 aagcaacagc cacttaatca aatctaaacc ccactcccca caccgtatga atatacacag
    30961 catgtgcctt tgctagtttc cctcgaagat tggaaatatg catattcact gttttctcac
    31021 tatcaaaata tggttcttgc caaactgttt cataaagatt tgctcgcgaa aatacttttt
    31081 tcggatgttc taaaaaaagc ttcagcagat cgaattctct tcctgtcaaa tggacagcca
    31141 caccattcac atttacatcc ctcgactctt tgtccagtaa gatttccttg taatagatgc
    31201 gttgcgacag atcttttact tggacagaag tctgatgacg aagttggatg ccgattcgta
    31261 acaacagttc ttcgatatca aatggtttcg tcacatagtc atccgctcct gctgccaaca
    31321 aggaaacttt atctctttga tcgctttttg ctgaaagaac gatgatcgga acattcgatg
    31381 tctgtcggat ttctgctaac aatgcttcac ctgaaagccc tggaagcatg agatctagaa
    31441 ggatcaaatc gacaggatat gtgcttaaca ataattttcc ttccgtacct gaataagcat
    31501 caagaagttt gtatctctgt tcaagaacct cctccagtaa agaatgaaca gcttcatcgt
    31561 cttcgataat caaaatagtt tccagcagga acacctactt aatgtcagat ttttggaaaa
    31621 taaacattcc tagagctgat gataccacga ttgttactag actgaccaga atcattttcg
    31681 gtgcttgcga ataagttgct tgttgcgcaa agtccgtata catgagccac gtttccgtaa
    31741 accactccgt tagaaattcc aagcctttga tttttgcgaa aacaccggga acgaaggccg
    31801 ttcccatgat ataaagaaaa gaaagaagca aactgacact ggtacttcta gtgatgaatg
    31861 tcaaagccac tggaacagat aatgtcgcta atatcagcag aagcaataca ggaaatactt
    31921 ttagaacata atttccatag tttttgattc cctcaacttc accaaatccg aaagcaagtg
    31981 tgtaagcaat catggctagc cccgtaatca aaaccacgcc aagtgttgtg atcaaaagcg
    32041 taacgagaag tttcgacaca taataagtca ttcgattccg tcctaaagat aacgtattgc
    32101 gaatcgtacc gttttgaaat tcttccccaa tataaaagca cgcaaatgct gcgaaagcca
    32161 gaagaatcca tgcagccatt ttttccacca ttaaaatccc tgttgcaccg ttgatgactg
    32221 tatccgttgc ttggtttaat tcattgccgt attttgtatg catataccat tcgttccaag
    32281 aataaaaaag cggaagtacg accatcagtc ctagtgagac ccacatttta cggctcttcc
    32341 atacttttat tttttcagca gttaatagat taaacatgtg gattgcctcc agttaattgt
    32401 aagaaataat cttccagttt ttgcccaact aggtggatac catccacagg aacttttgcc
    32461 tctaccaatg cttgattgat ggctgctact tggtcaattc cttcaaaaag ctggataaca
    32521 cggctagatt ggacaaaata atccctataa ccaagagagt cgagaaccgt tactgcttgt
    32581 tccgtcgccg aagtgtcaat ttttataaat tgacgagatt cttgtgccaa ttcttctttc
    32641 gataattctt tgatcaagtg tccttcatgc aaaataccgt agtgagtggc gatctgtgac
    32701 aattcatcta gcaagtggct agaaagcaag acggtgatgc cccgttcttt taccaaacgt
    32761 tggatgattt ctcgcatttc tgccatccct gctggatcca tcccattcgt cggttcatcc
    32821 aaaatcaaaa attctggatt cgtgatcagt gcatttgcaa tagctaaacg ctggcgcata
    32881 cctaaagaga aattctttgc tttctttttc cctgtatttt ccaatcgcat cattttcagc
    32941 aggtcttcga tttctctatc cgacacaccg ccgttcgctg cttggatacg taggttatct
    33001 cttgcagtta attctggata aagagcaggg gtttcaatcg attgacccat acgtcgtctt
    33061 gctgcctgca aatccgtcgt tccaaataat tcgatcgaac cttcatcaat cgagatcaaa
    33121 cccataatag ttctcataaa ggtagactta cccgctccat tctccccaat caaaccatag
    33181 atcattccac gtttgatttc gatcgatact ttatctaatg ctttggcagc accgtatttt
    33241 ttggttatac cagttgcttt caagaccgtt tcatttccaa aatccatact tatccttctt
    33301 tcttattgat aagtgaagta tagttgacgg gtataaagaa agcagccaga aatgtataaa
    33361 gaaagtgtaa attttccaat tgaatttcaa gaaaaatcag actgtctcgc agacgtggtt
    33421 gagttgtctg ccaatcaaag agcgcaccaa acagtgctaa tccaagtgga ctggccacca
    33481 tggtaatcgt tgttatcgtt gacatcagac gtccttgtat atgcgttgga acttctcgct
    33541 gaagccagac catataaaaa gcaaaagaaa acattaatcc ttaaaaacgg acttatgaaa
    33601 aataatacat gccgtttggg atttacaatt agctaccaaa taagaggtta tgacaaaaga
    33661 cttttcacaa tcccttcttc cgaataaacg gtgttctcct gccaactcct cggtcacaag
    33721 tcacaatatt tatcaaacat gagaagcaat ttttgtaaat tccttcttat ttcttgaagc
    33781 tgaccacttc tgtcacaacc tcagaagcgg ataaataata ttgtttgatg atactttatc
    33841 tttttcccgt ttgtttatag atcgaatgat attatagacc aagaatccta cctattaaca
    33901 tagctaaaaa cacaaaaaag gcactttcca aattgtggaa agtgccagaa aagttaggat
    33961 taaggtaact gcgctcagca ttgcttcgcc cagtacgatt ctcgtaactc gtgaactcga
    34021 acgataatcg caattaacgt tttgagaact gtgaagcttt acgggctttc ttaagacctg
    34081 gttaaacaaa actctatttt attgcatttt acaggaatgt atttcatgcg aaattaataa
    34141 gtctttgttt atagagtttt actagggtat aaaaagttgt atgttatcat tacctcaaaa
    34201 cattacctca gcttggcgag ttggttgatt gaagaataaa tcatttatct agatatctca
    34261 ataaaaaatg atttctaaga aacttgtatt ttcgtgttat tatttttttg ccctatcaaa
    34321 atttctaatt cttctagcgt aatatgatta tgacacataa tcgattcaat tacttcaata
    34381 cctatttttt tcgagaaaga tcccttttgt tcactcacaa aaatactttc agaagacata
    34441 tataaggaac gaagttttgg aattagtata tcgtacaaat agaccaccat aaaatcgtcc
    34501 ttgcaaccat tctcaaaaag aattaaattt gctgattgtg ttacatatat cactgaatta
    34561 cattcatggt caattccaaa aacaatgtct acttcatttt cttttctaat tcgcgcagtt
    34621 acatttttca aactttcacc attacttata aatgctttag ttactgtaag ctctgatgtt
    34681 tgttcagata ttccttttat aatattttgt tcatcagcca cattccccat aaatccgccg
    34741 attcctttta agtaataatt ctcacctaag agtcgcttgc attcactgaa ttgataaaaa
    34801 aaccagttaa aaaaatcttc tggaaaaata aattctgatc gaactttatt tatattttta
    34861 gctcctatta attttataac tcgattaatg ttcacttgct gcgatgaaca tataactgaa
    34921 taaattgagt tgtttaattc aaaaaaacaa ctttaacata tgcatcatta attcgttatt
    34981 ttttaggtag atattgatca ttgttatctc tctttttttg cttcccatac tccaacttag
    35041 aaattgccgt tataaaattt attttaaagg tgactttttc ccctgaagta tcggtagtag
    35101 gaatacttaa tacctcagct tctgaggagg tatcccaatt atagttatac tttgcatcat
    35161 caccataaag aacaattcca ttttttttat cttgtgccgt tagctttact ggttcgctta
    35221 cttttcttgt taatatcttg gactttacat catcgatatt gcttatttta tcaagcttaa
    35281 ataatttaca aattaatttc gtactagttt ttatattt
//
LOCUS       DACGLW010000019        34660 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.019, whole genome shotgun sequence.
ACCESSION   DACGLW010000019 DACGLW010000000
VERSION     DACGLW010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34660
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.019"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            complement(313..2205)
                     /locus_tag="IXT53_001011"
     CDS             complement(313..2205)
                     /locus_tag="IXT53_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4904856.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2202..3929)
                     /locus_tag="IXT53_001012"
     CDS             complement(2202..3929)
                     /locus_tag="IXT53_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4904857.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3945..4595)
                     /locus_tag="IXT53_001013"
     CDS             complement(3945..4595)
                     /locus_tag="IXT53_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ4904858.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4799..5095
                     /locus_tag="IXT53_001014"
     CDS             4799..5095
                     /locus_tag="IXT53_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HAQ4904859.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5287..5907
                     /locus_tag="IXT53_001015"
     CDS             5287..5907
                     /locus_tag="IXT53_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HAQ4904860.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(5999..7165)
                     /locus_tag="IXT53_001016"
     CDS             complement(5999..7165)
                     /locus_tag="IXT53_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ4904861.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7177..7530)
                     /locus_tag="IXT53_001017"
     CDS             complement(7177..7530)
                     /locus_tag="IXT53_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAQ4904862.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7658..8680
                     /locus_tag="IXT53_001018"
     CDS             7658..8680
                     /locus_tag="IXT53_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HAQ4904863.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            complement(9119..9553)
                     /locus_tag="IXT53_001019"
     CDS             complement(9119..9553)
                     /locus_tag="IXT53_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4904864.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9663..11522)
                     /locus_tag="IXT53_001020"
     CDS             complement(9663..11522)
                     /locus_tag="IXT53_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ4904865.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(11640..12799)
                     /locus_tag="IXT53_001021"
                     /pseudo
     CDS             complement(11640..12799)
                     /locus_tag="IXT53_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
     gene            complement(13106..14530)
                     /gene="araA"
                     /locus_tag="IXT53_001022"
     CDS             complement(13106..14530)
                     /gene="araA"
                     /locus_tag="IXT53_001022"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HAQ4904866.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     VADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14546..15244)
                     /locus_tag="IXT53_001023"
     CDS             complement(14546..15244)
                     /locus_tag="IXT53_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAQ4904867.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(15201..16862)
                     /locus_tag="IXT53_001024"
     CDS             complement(15201..16862)
                     /locus_tag="IXT53_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HAQ4904868.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            17080..18480
                     /locus_tag="IXT53_001025"
     CDS             17080..18480
                     /locus_tag="IXT53_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HAQ4904869.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18732..19811)
                     /locus_tag="IXT53_001026"
     CDS             complement(18732..19811)
                     /locus_tag="IXT53_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ4904870.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(19897..20856)
                     /locus_tag="IXT53_001027"
     CDS             complement(19897..20856)
                     /locus_tag="IXT53_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HAQ4904871.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDTLIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(20868..22391)
                     /locus_tag="IXT53_001028"
     CDS             complement(20868..22391)
                     /locus_tag="IXT53_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAQ4904872.1"
                     /translation="MDEEVKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHE
                     TADGDGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIE
                     PNSFGLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQ
                     KKPFGVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPR
                     LPSYPEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFD
                     RQIEEIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMED
                     TLLFGSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAK
                     GQVLKQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLL
                     DQGEHYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22401..22964)
                     /locus_tag="IXT53_001029"
     CDS             complement(22401..22964)
                     /locus_tag="IXT53_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HAQ4904873.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(22970..23833)
                     /locus_tag="IXT53_001030"
     CDS             complement(22970..23833)
                     /locus_tag="IXT53_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ4904874.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(23847..24743)
                     /locus_tag="IXT53_001031"
     CDS             complement(23847..24743)
                     /locus_tag="IXT53_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ4904875.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(24918..26189)
                     /locus_tag="IXT53_001032"
     CDS             complement(24918..26189)
                     /locus_tag="IXT53_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ4904876.1"
                     /translation="MKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTLTFWNGF
                     TASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFIALNYPD
                     FAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMYMYWNND
                     LFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLANGGKLV
                     NDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIEINGPWL
                     NNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFLKYWNTT
                     EIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQISAINNDI
                     INPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(26179..27696)
                     /locus_tag="IXT53_001033"
     CDS             complement(26179..27696)
                     /locus_tag="IXT53_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HAQ4904877.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            27894..28784
                     /locus_tag="IXT53_001034"
     CDS             27894..28784
                     /locus_tag="IXT53_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HAQ4904878.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(28857..29681)
                     /gene="nadE"
                     /locus_tag="IXT53_001035"
     CDS             complement(28857..29681)
                     /gene="nadE"
                     /locus_tag="IXT53_001035"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HAQ4904879.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(29693..31165)
                     /locus_tag="IXT53_001036"
     CDS             complement(29693..31165)
                     /locus_tag="IXT53_001036"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HAQ4904880.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(31802..32164)
                     /locus_tag="IXT53_001037"
     CDS             complement(31802..32164)
                     /locus_tag="IXT53_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAQ4904881.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            32387..33139
                     /locus_tag="IXT53_001038"
     CDS             32387..33139
                     /locus_tag="IXT53_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ4904882.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(33279..34202)
                     /locus_tag="IXT53_001039"
     CDS             complement(33279..34202)
                     /locus_tag="IXT53_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ4904883.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(34217..34489)
                     /locus_tag="IXT53_001040"
     CDS             complement(34217..34489)
                     /locus_tag="IXT53_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4904884.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        10229 a         7213 c         5980 g        11238 t
ORIGIN      
        1 caagaggttg tgagcctgcc gtttaactcc gagtcacaag gagcaatttc aaaaaacagc
       61 ttctcaggtt tgttgactat tgtgacttgt gaccgaggag ttggcaggag aacaccgttt
      121 aaggaagagg ttgtaaaatc agcggtttga cctgagtatt agagatacaa aacggaaaaa
      181 tagcttctca tattttgccg ttttttgagc taataccgaa ggacagctga ttgaacaccg
      241 tttattcgga aaaagggact gtgacacgac ttttgtcaca gcccctaaaa aaaataataa
      301 atttgtacta ttctatgaag cttcttcttc agaaaactga ctgttataaa gatctgcata
      361 gaatccgtct ttttccatca atgtatcgtg gtttcctgtt tctacgatcg aaccatgatt
      421 catcacaata atgttgtctg catcgcgtat tgtagacaag cggtgagcaa caacgaaact
      481 tgtccggttt tccaataggc ggttcatcgc tttttgaatc agtatttctg ttcgtgtatc
      541 tacactagat gttgcttcat caaggatcag gacatctgga tttgctaaga atgcccgcgc
      601 aattgtgatt agctgacgct gcccttgcga aatattgctt gcatcctcgt ttaatacagt
      661 gttatatcct tctggcaatt tacgaacaaa atcgtctacg tgagcagctt tagcagcctc
      721 aatgatttgt tcgtctgttg catcgtcgtt cccatatttg atattgtcat agatacttcc
      781 tgtaaacagc caagtatctt gcagtaccat tgaaaactgt gcgcgcaatt catcacgtga
      841 aagatcacgg gtatctacac catcgtaacg aatactacct ccagaaacat cgtagaaccg
      901 ttcaagaagg ttgatcagtg tcgtcttccc tgcaccagtc ggacctacaa tagcgaccat
      961 ctcaccaggt ttgacatcca agttaaaatc agtcattagc aagttatctg gtgtgtagcc
     1021 gaattgaaca ttttcaaaac gaactttgta tggtgtatct ttttcttctg gaaggccaga
     1081 tttttcattg gacatctctt cttcatccaa cacttcaaat acccgttcgg ctgaagcaac
     1141 tgttgattgg atagtgttca tcaaacttgc aagttgagtg attggttgag agaactgtgt
     1201 tgtatattgc aagaacgctt gaacatcccc taatgtcatg ttcccatttg caacttttac
     1261 tccgcctagt acagcgacga acacatagcc tagattttta acgaaattca tcaacggcat
     1321 gatgattgcc gaaataaatt gagctttcca accagcggca tacagattct gattttcttt
     1381 ttcaaatact tcctggtctt tttcagtatg attgaatgtt ttaacaatgg tatgaccacc
     1441 atatgtttct tctacttggt tattcaataa acctaaactt ttttgctgat ctgcgaaaaa
     1501 cacttgtgat ttcggcgcta cgatcatcac gatgatcaag cttaaaggga tcatcaataa
     1561 cgcgactaac gtcaattgcc agctgattga aagcatcatc acgatgaccc caatcaatgt
     1621 gactgcacta gttacaaatt gagtcaaatt ttgctgcagc gtgcttgcga tgttatccat
     1681 atcattgatt gctcgagaca tgatatcccc atttgtatgt gtatcataat aggaaatcgg
     1741 tacacgattc attttggctt ccaaatcatg acgtaattca taaactgttc gttgggaaac
     1801 acgtgtcata ataaattgct gtaaaaagtt gaatacagca gaaatcaaat acatcacgat
     1861 tactgtcgca atgatctgcg cgattttatc aaagtctatc ggaaattttg ttacttgttg
     1921 gccttgttgc atctgttgag cgccagccat tacacctttg aatatttctg tggttgcttt
     1981 ccccaagacc tttggtgttt ggatttggaa gatcacggct gctattgcta gtacaaatac
     2041 agcgatgatc gcaaccatac gtttagacat atagccaaac aatcggcgta ccgttcccca
     2101 gaaattttta ggcttttcac cttttacacc gagatttctg ccaggtccac catgtcctgg
     2161 accgcctgct ggtccatgag agggacgtgt gtttgtttta ctcatgctaa gtcctcctct
     2221 cttagttgtg aatgaacgat ttcttgataa gtttcgtttg ttgctaatag ttcttcatgt
     2281 gttcctttgc caaccagttt tccttcatct agcacaagga tcatatctgc atccattact
     2341 gtacttactc gttgagcaac taaaacggtg atactttctt tcatattcgt tttcaatgct
     2401 tggcgaagat tggcatccgt tttgaaatcc aacgctgaga aggaatcatc aaacacatag
     2461 atatctgctg ttttgactaa tgcccgtgca attgccagac gctgtctttg tccaccagag
     2521 aagtttcctc ctccttgttc cacatggcta tctaagccat cttccatctc tgaaacaaaa
     2581 tctttagctt gtgcgatttc taatgctttc caaatcattt catctgttgc atccggctta
     2641 ccatactgca tattgtctcg gatcgttcca gagaacagaa ctgctttttg aggaacaaag
     2701 cccattaatt ctcgcaaatt atattgagtc atgtcactaa tgtccgttcc atttatttgg
     2761 atcgaaccag attcgatgtc atataatcga gggatcaaat tgactaaggt cgttttacct
     2821 gaaccagtcc cccctatgat tgcgacgatc tcgcccgact tggcttggaa atcaatatct
     2881 tccaatgcaa gtttttctgc tccatgatat cgataattga catgattgaa ggccaatgtt
     2941 gcgttttttc cagcaaaaga aactgtttta ggattttcag ggtcatggat accgattttt
     3001 tcatccaaca cttcattaat acggtcagct gaagcttgtg cacgaggcac catgataaaa
     3061 atagcggaaa gcatcataaa gctgatcaag atctgcatcg cataagtcat aaatgcaatc
     3121 agattaccga cttccaaagt catatctgct atgtagtgtc caccaaacca agtgattgct
     3181 acgtttgtac cgctcatgat caatgtcatc attggcatca tcaatgcgac gatcgtatta
     3241 actttgattg ccgtatttgt ataatcttta ttcgctaaat caaaacgatt ttcttcaaag
     3301 cgtgtcttat cgaatgctcg aatcactctg acccctgtca atccttctcg aaataccagg
     3361 tttaaacggt cagttttctt ttgcatactt ttgaataatg gaacagcaaa gtacatgatt
     3421 ccgccgatca aaataatcat tactgggatg acaactaaga agatttttgt caactgatga
     3481 tctttttgat aagcaagtat gctcgcgcca atcaacgtga ttggtgcatt gatcatcata
     3541 cgtaaaaaca tttgtgtaac catttggatt tgagtcacat cattggtcgt tcttgtgatc
     3601 aaagaggccg ttccatattt atcaaacgca tcttttgtca gactctctga ttttttatag
     3661 acatccgtac gcaattgctt acccagtttc tgtgattcac gagtagcaaa aaatgtattt
     3721 gcgatagcag ctaagatact gatcaaagaa aagccgatca tcacaaaacc tgtatgccaa
     3781 atataatcga catctccttg cgctacccct ttatcaataa tattggaggt aagtgtcggc
     3841 aaatataaat ctgccagtac ttggatcacc atgaaaactg cagctgcaat tgctgaagtc
     3901 aaggagatcc gttttacgat ttttagcatc tagttccctt ctttctattt atatactccg
     3961 tttttcagcc aagaaagttt caaattgaat tcactgattg cttgctctac tgagtaatct
     4021 tcagatgttg aaaataagcg atacgaatgc gcaattgttg taaatacagt attcatcact
     4081 aattggatca gtaattccaa ttcatgatca ttttttactt tcagcttcgt ccgatcgatc
     4141 aattcaagca actcttttgc atcttgtttg tgttctttat gtggatcgtg aggcgttttt
     4201 tggcgactcg tatgaggagc gacttttctt gaggcacggt aatccatgtt cataaacaag
     4261 ttacgataaa aagaagcatt ctcgccttcc aaaatctcat aaatcatttt agaaaaatag
     4321 gattcgaatc ctttgaatag atcaccatcg gattctttca ttagttgtaa catatcttta
     4381 gagctgtcac gtctcattgt ctcaaagtaa taaaaataaa gatcctcttt atcttcaaaa
     4441 tactgataga aactgcctct agggatatcc gctaatttga cgatattagc aatcgaagct
     4501 tcttttaagg gtactctaga aaactcgatt ctcgcagcct cgattagtct tttttgtttt
     4561 tctttaggca aatggaaaaa tgtctgtgtc ggcatatgga ttctcctttc tttcaagatg
     4621 acacattgtc atatgacgct atgtcattat aacacctcct acctgttttg caagacattt
     4681 gcatagtttt tttcattatc atccgataca ttaaaaaagt gataagagga atccttccat
     4741 ttgaattaat gagagttgcc cattaaaata gccacatact aaacaaagga tgagataaat
     4801 ggcccaatca attagtgtcc aagacttcaa taaattgcct ctcgatgctg acagcatcgt
     4861 tttagataca agaaatacct tagattacac aaaaaaccac ttaagccact cgctttctat
     4921 ccccgctgct atcttgccaa gacaattaaa aaatctagat ccaaagaaac tttattatat
     4981 tctaagctat acaggcaggc gatcagaagc attagccgat caacttactg ctaatggttt
     5041 tcgtgctgtt tatatcattg gtgggatgga agctcttcag tcgagtgttg cttgaatatt
     5101 gcttgttaga ttcaaaagag gctgtgacaa aaaatcgtgt cacagcctct tttataccct
     5161 gtcttttttt agttatttat tcttcaacct tatcaatatg acttgatagc cttttctttt
     5221 tcgagtaaac tgattttgtt agtcatagtt ataacaatca tagcaataaa aggagaaggt
     5281 aagcatatgg catctgtttt agtagtcaaa ggtcatcccc taactgcaga agaatcgcgc
     5341 acagttaagg cattgacgtc ttttttaaca agttataaag aaaatcatcc agatgatgaa
     5401 gtgactgttt tggaacttta tcgcgatgat attccggaaa tcgatgaaga attattatca
     5461 ggttgggaag cattacgtgc tggtgcagaa ttcacttcct tatcagaaag tcaacaacaa
     5521 aaaattgctc gtttcaatga attgactgat cagttccttg ctgctgataa aatcgttatc
     5581 gcaaacgcct tatggaatct aaatatccca acaaaattaa aagcttggtt cgataccgtc
     5641 aatgtagctg gaaaaacgtt ccgctataca gaaaacgggc cagaaggatt agtgacaggt
     5701 aaaaaagcac tgcacatcca atctaatggc ggtgtataca acggacaaga ctttgcctct
     5761 caatatgtaa aaggtatttt aaactttgta ggcatcgatc aagtagatca gctattcatc
     5821 gaaggaatcg attatgaccc tgaccgtgca gatgagttga tgcaaaatgc attagataaa
     5881 gctgctgctt taggtaaatc attttaaaaa ttgagataaa tgcatacaaa agagacagag
     5941 gaaaaaatct tcaaagcagc atttgtgcct tggatatttt cccatctgtc tctttttgtt
     6001 attctttctg ctttttcaac ggattttctt cataaagaga agagattgtt gtgtaccatt
     6061 caaaaacatg agtaatcact actttagctg ctgcataaac aggaatcccc aagatcacac
     6121 cgatgattcc gaacaatttc ccagaagtca gcagaactaa taaaatcgtt actggatgga
     6181 ttgctaactg attccctagt actaatggag agatcaagcg tccttctatt gtctgctcta
     6241 ctgcaaaaac gatcagtact ttgatgatca tgaatggacc tgctacaatg gctaaaaaga
     6301 tagccggaat catcgctaaa aatgatccta agtaagggat caggtttaaa aaacccgcaa
     6361 taatccccaa cgtgacagca tactctaaac caatgaccgc aaaaccaatc ataaacatga
     6421 ttgccacagc aaaagccaca gttagttgtc ctcttatata agaagaaact tgactattca
     6481 tttcactcag cacattcaac gtcggttttt gcatgcgtgt cggtaaaaat ttgacaaaat
     6541 aaggtgctaa ttgtttaccg tctttcaata gataaaataa gataaacggc atcgtcagca
     6601 gagccactaa aatagtcgct accgcgccga caaaacttcc aagactctga actgtactct
     6661 tagatatatc ttggatcata tcgcctgccg aattcatgaa cttatcacct gctgtttcca
     6721 actgttcgcg aaattggttg aacagcgggt cccgcaaaat ctcggttaat ttattatcaa
     6781 tcgtatctac atattgaggg aagttactga tgaagctcac tgtttgttcc tgaatcttag
     6841 ggatgatcac tacgctcccc catgcgatta gtgcaacaac caaaacaaac agcccaatga
     6901 tactgtaaag ccttggtact tttcgctttt caaagtaatc aactaccgga ttcatcaagt
     6961 aatacaagat tcctgctaaa atgataggca gacctacgat tgctaaaaat tgccagattg
     7021 gtgcaaacaa atatgaaact tttgtaaata aaaagacgat cagcaatatt aacaatacaa
     7081 caagtaaggc tgtgactact tgattattta aaaaccagcg ccaaaaccac gataccggtc
     7141 tgttcttctt attttttcct tccataattc ctcctgttag ttataaaaaa tttggcttcc
     7201 caaaccaata gcaggcagtc tatgcggatg aagatagatt ccatttgcaa tttgtccttc
     7261 ttctggacaa gcatcaaaaa tcaatgatac gtgagctact gtctttaaat tcccattggc
     7321 catcggaccg acataatcga tcgtatatac ctgatcatca aaagcaattg ttccgcctgc
     7381 tttcaaagaa aaaggttcgt cttttgatac ttcttgaatc accgaatatt ttcgtaaggc
     7441 agctgttgct gttttatcga acaaaatcag catgggttct tccttgttta ttgcttctgt
     7501 cccaatttct gtaatctttg cctgaatcat tgcactctct cctttaattg ctttcaaaaa
     7561 cagtataaca cattctcatt ctttagccgt gtggaataat tatgtgatag aataaaaacg
     7621 aaaactattt cattacaatt aggaggaatc agctcatatg atcgaaaaga taattttagg
     7681 aacctatacg cgtcgcgaaa gtaaaggaat ctatacgatc gaccttgata cagaaaaaga
     7741 agaattagcc aacctttcat tagctgttga agaaaatagc cctacctatg tagcaaaaag
     7801 taaagctggc aatctttaca cagttactag tgtggatgga ttaggtggtg ccggtgcgta
     7861 cgataaggat tttcattttt tgaatgcggt aacagagtct ggcgctcctt tgtgttatgt
     7921 agcggttgat gaagcacgtc aattactata cggggcgaac tatcataaag gtgaagtaaa
     7981 tgtctaccat atattaggag atggcgaact gaaagcagca gattcaatat tccatcaaga
     8041 agcgacaggt cctcataaaa accaagatca tgcgcatgtc cactatacgg atctgactcc
     8101 tgaccaacga ttagtcgtat gtgatcttgg tacagatcgt gtgtatactt atgatgtgtc
     8161 cgaaaacggc aaattgaatc ttgtaactac cttcactgca gagccaggaa caggccctcg
     8221 ccatcttgtc tttcatccaa atggacaatt cgcttattta tttggtgaat tagacagcac
     8281 agtccgcgtg ctttcttata ataaagaaac aggtgctttt gaagaattac agaaaatatc
     8341 gaccttgcca gaagaattcg acggtgaaaa tggaggggca gctatccgca tttcaaatga
     8401 tggcaagttc ctttatgctt ctaaccgagg acataactcg attgctgtat tcgcagtttt
     8461 tgaagatggt tcccacattc aaaacaagca aatcatctca acagaagggg attttccccg
     8521 cgatttcgca ctagatccaa gcaataattt tgtcgtgtgt gccaatcaga acaccgataa
     8581 tctgacatta tatcgccgtc atcctgaaac aggattactg gaattactga aaaaagatgt
     8641 ctatgtacca gaatgtgtct gtgtttattt tgatcgttag taagaaaagg agaatgaaat
     8701 gaatcagcaa gacgtattag acagactacg ggaagaacta aatattcctt tctttgatgg
     8761 gaaaatcgaa gataaagagt atagcgaaga ggaatatcag aaactcaaag ccgatttaca
     8821 gaactacttt gaacaatatg tacagaatgt agaaaactaa tcagctttcc atactatctg
     8881 aaaagaacat caggacaaga cttttccgtc tatcctgatg ttcttttcta caaataaaaa
     8941 accgccgaga tggttagact cgacggaaaa ggagtattta ctcgattttg agtaagtaca
     9001 aggttgttgt tggttatagg tatgtcatta tcataaagga ttttgatcac tctatcataa
     9061 aggtaatatt acatttatat tagataactc tagtttttat tgttatcaaa gatttctttt
     9121 attttttcaa caacaaatta gtcatttttt ggatggcagc tgtcaattgg tctgtcgctt
     9181 ggtcttgttt tgcttcagat gtattgaata gatcttcatc tacccctgat tcaagatcga
     9241 tactttggat catttcttca cagcctgcta catatgaaag ataagctttt tcaaaatttt
     9301 tatgaatgcc cattgctttt gctggtggac gcaaagtact gactttcata agcattgccc
     9361 gatatttttt tgttccttct tcaaataaca ggatgatctc ttcaagagtc gatttgtcca
     9421 gttcacttgt ttttccttca tcaattgcgt ttctgatcac ttcatatttc ggatgcattc
     9481 cttctccgat tgcttctgtt tccttgacga tttgatcgat ggactgcaca taatagccaa
     9541 tattcggtaa catgttcgtt ttcctacttt ctccattgtt gttattattt attctacttg
     9601 tttttatcct caaatgacaa gtacttgaaa aaatttaatg gtttctatac agctcgccat
     9661 tcttattcgt gctcaaagaa accatctgct aaactatatt gtttccctgc gacaatgtca
     9721 tattggatca attgatattc gtcgtaaatc tcttgttgcg agcgattgaa ttgcgcttct
     9781 ttataccact gtccgttttg atagtacagt tctcgttcaa acgccggaag ttcctgttct
     9841 aatgataaaa gcagctgata aaagccattt gttgtctgat tcgtctgttc cattagattc
     9901 gctgcaaaat aaaacggact ggtgattgca tcgtgagtcg tttgcttttc taattctcct
     9961 ttactgtcga acataagaaa ttgtgtttca tgcaatgttt gtttgtcatt ttttgcttgg
    10021 atcgtatctg aatagatccc tggaagatga tccccccaaa aaacaactaa agtccttcta
    10081 gaaagattct tcaattcttc cgtgaactgc ttcagtgccg tgctgctata ggaaatatct
    10141 tgcagataat tctctaagtc aagtgtccca cttccatctg aagtttttgc aggatagtct
    10201 agattatcat actttccatc gtagggcata tgtgtctgca tcgtgactaa atgaatgaac
    10261 tgaggtgttt tttcttcctt gagaagtgtc attacttctt tgtaagctga ctcatcagaa
    10321 atatatggat tattctctat cgtatccgta taggtcatcg tacgctcgct gatgaactgg
    10381 tcaaatccta atgtctgata aacatcctct cttttataca tcgaagtatt gtatggatgg
    10441 atcgctgtcg tctggtatga ttgtgcgttt aacgctgaaa cgatagaagg aagctgatcc
    10501 atttttggta caagcatcgt gtaaggggtg gtgagttgcg cattaaataa cgccatagaa
    10561 aaaccagtca atgcttcaaa ttcgatattt gctgttccac cgccatagtt ttgagaaagc
    10621 atattgccac tatatgtttg atcagctact tcataataat ctgccaatgg ctcaccagat
    10681 acttctacgc cgtttaatcg actaggatct gagaaacttt cactcatcac gaacacgatg
    10741 ttcggcgact cttcctctac agttttattt ttttcatcgg ctagcttctg gtatttttca
    10801 gtgatttctt tgatcttttc tttcgaatat ccttcaggtt catccattgg ttctaccttc
    10861 agattgtaca aaaacccacc gataaaacca gtgttatagt agttcatttt ctggctgtaa
    10921 ggtatccata gtgccgtttt attatatgct tttcgcaaca aattatcggg attgttgaaa
    10981 ttactgatat accctagcag tccaattgcc acaagcagtg tcgttaatcg gatcaattgg
    11041 aatcgccttc cttttttcag gcttcgaaac atgtaccagc aaaacaatcc tatcacggtt
    11101 cctgcggcag ctaagatcac tgtaaacagc atagtgccgg tcatatcttt cagcaatccg
    11161 atttcagtga tcatcttcaa atcgtctgga taaatcggtt cttgccgata actcattttc
    11221 atataatttg caaaacctaa tacaccgatg gctacgacat acgtaaggga acctaaaggg
    11281 acagatcctg ctagtgcaat cagaaaaata agaataatca gcaagacgag acaggctaag
    11341 aagaattttt ccgtatgcca agaaaaagca aatttcaacg caaggtctac ggatagatca
    11401 ttttgacacc actgcaaaaa taaattacta aaaacaacaa gaaaaaagat actgatgatt
    11461 gctgctgttc cttttaaaat ctttttctgt ttggtcgaaa gtctgcttat atatcttttc
    11521 aaatgcttgc tccttcattt ctatcaataa aagaatgttg cttctcttat tttagttgta
    11581 cgaaattcga aagtaaagaa cggaaagaag aattaatgaa atccaaatat tctctttctt
    11641 tatggccttt ctggtttctt taataagcgg acctttcgta ctccagaaat tttttctagc
    11701 tgttgtttca cttgattgag ctgttcaaaa tcagtactgt ctatgtcaat caatatgtag
    11761 acatgctcat tcttatggtt acgcgatata tctgcgatcg ctagctcgtt ctcattcagc
    11821 agagaaaata tctctgctaa tatattggtt ttcttttgat aaaatatagt gaatcgaaac
    11881 ggcgcttgga aaaacattcg tgcggatgga taattgaccg cttctctaac cgtaccgaac
    11941 aaaagataat cttttaaggc ttcaactgcc aaacgtccgc tgtctgccaa agcttcctga
    12001 gtcgatcccc tagatgaggc aacataagta tacggtcatt ttctaagaac tcttcttctg
    12061 gaaaatcagt caagtattta gccagatgtt gtttttccaa tgcttgcata acagcatatt
    12121 tatcaacaat ttcacttctt cctacattga taagtacagc atcttttttc attttttcta
    12181 tattcgcctg atcgatcaat ccttttgtat cttctgtcaa cggcagcatg actacgatgt
    12241 aatccgaggt agaaagaact gtttctaaat ctgcttgccg aacataatcc aactgggcat
    12301 cgcggataga ataacccaag acatccatcc ctaagctata gcaacttagc gctacttttg
    12361 tgcctatcgc tccaagacca agaagaccga tggttttgcc ttgcaattct cgtcccacat
    12421 aagcacttct tttgttttct gcctgttcta aaatattagg acctgttagt gtttgtacca
    12481 tacgacttgc ttcgattatc ggacgggaag ataagagcaa acagcacaaa acaagctctt
    12541 ttactgcatt agcatttaca cctggtgtat tcatgacgat cgtcccgttt tcagaagctt
    12601 tttcaacgtt gatcgtgttc accccaacac cagctcttga aattgctagc aaatctggtg
    12661 taagccattc atctttcact ttcgtactac gaataactat tccttcggct ggcaaatcat
    12721 ctattatttt gaaatattcc ccttgtaact tttctatctc ttgttctgga atagcatcaa
    12781 tcaaatgaac gtgatacata aaaccccctc cttactaaca agtatctcga aatttctgca
    12841 attagacaac tgtttaccat ttgacttcct agaagcaaaa agagcagtta gagcagagta
    12901 ggctgagtga taaaaataag gctatgacaa gttactgcca taagccttat tttcgagagc
    12961 aaaatattga gggtgagata tcttgataaa tggtgttccg ttagctgaca ttgataataa
    13021 gactaaattt caaaaaagtt gaaaagcaaa tttcgtcaat ctcttcttat ttcttaaaac
    13081 taagtggctt tttcacaacc tctttttatc taataaccac tgtttccaac tcaactaatt
    13141 ttgcccacgt ttcaatttga tctgttgtta gatttagcga gacaacggta tgatgaccgc
    13201 cacctgcctg aatccatgaa tggacacctt catggaaatt cggtttagtt ttccataaca
    13261 cacgtgcaac aggtaaatta ggtgcttcag tcgttggttc aaaggcttct acttcattga
    13321 tcagcagttt ataatgcgta ccgaaatctg ccaccgatac gactacccct tctcctgctt
    13381 tcccatcaaa tacaagacgc gccggatctt ctctatctcc cattgataaa ggagaaacaa
    13441 cgattcttgg tttcgtcgca gcaagcgtcg gatctacttc catcatatgg gattccaaga
    13501 ttgcttcttg gccactagct agctcgtaag tataatcttc catgaatcct gtatctttcc
    13561 ctcgagccat gattttcagc aaccgatcaa tcgctgcagt tttccagtcg ccttccccag
    13621 caaaaccgta tccttctgcc atcaaacgtt gtacagctag tccaggtagt tgtttcattc
    13681 catacaggtc ttcaaaatta gtcgtaaaag cgttatagcc tccttcttct aagaaacggc
    13741 gaataccgat ttcctgctga gcttgtactt ttacatgttc ctcccatgtc ttttcttcat
    13801 aatcaccata gtcgaaggta tacaattcct tgtactcttc aaataattga tcgacttctt
    13861 cgtctgatac gcgatcgatt acttgtacaa gatctccgat accaaaataa tcaacagtcc
    13921 aaccaaattg aatctgtgct tccactttgt ctccttctgt gacacccacg tttctcatgt
    13981 tgtcaccaaa acgagccact tttattccaa aactttcatt ataggctact gcaacatcca
    14041 tccaatcagc aatctctttt tggatctcag gtcgtttcca gtagccaacg acgattttat
    14101 tttgtttatt taatcgagca ttgatgaacc catattcgcg gtcaccatga gcggattgat
    14161 ttaaattcat gaaatccata tcgatcgttt tccaaggaat gctttcgtta tattgcgtag
    14221 ctaaatgaag caatggtttt tgtagaagct ttgtcccgcg gatccacatt tttgctggtg
    14281 agaaagtatg catccaagtg atcacacctg ctacttcttc tcgataattg acctctttca
    14341 tgatttttgt gatcgtatca gcagtaactg ctaattcttg aaggcggatt ggataaggca
    14401 attggccatt ttcattcagt tcatcgacca tttcttgtgc atgtttcttt acttctcgca
    14461 gtgcttcttc cccatataaa tgctgtgatc ctacaacaaa ccaaaattct ttttctccaa
    14521 tattcaacat aattagccac tccttttatt tgttttgtcc gtaatacgca ttttttccgt
    14581 gttttcgtaa ataatgttta tctaaaatcc gttgaggcag ctcttctgaa aatgaattga
    14641 gctgtctggt aaataaattc atcttgcata cttcatctag tacgactgcg ttcattaccg
    14701 cactttgcgc atctttcccc caagtaaatg gaccgtgtcc gtgtaaaagt acccctggaa
    14761 ttgctaaggg atcaattttg cgttctttga atgtttctat gataactttt cctgtttcat
    14821 attcgtaccc gctgtcgatt tcctgttgtg tcaaaaatcg agcacaaggt accgatccat
    14881 aaaaagtatc tgcatgcgtc gtccccatcg caggaacatc caatcctgct tgtgcccaga
    14941 tcgtcgccca tgtcgagtga gtatggacga ttcctccgat ttgaggaaat gaacgatata
    15001 aaacagcatg agtaggcgta tccgaagagg gattgagttt accttcaatg atatgattgt
    15061 ccaagtccac aacgaccatg tcgtctgctg tcatctcttc ataactgact ccgctaggtt
    15121 tgataacgaa atatcgtgtt tcaggatcaa aagcgctcac atttccccat gtatatttga
    15181 cgagtccgtg tttaggcaag tcaagatttg cttggtatac ttcttctttc agttgttcca
    15241 gcattttcta tcactccttt ttcttgcaat tgatgaatcg ctgttttttc aatgtcgatc
    15301 ccctcacggt aacgtctcat aaatatctga tagcctgcta tttcttctgc tgaaggtgtg
    15361 atggtgattc cttggtcttg tccaaacacg tcttctgcca agaagttttc taaagacttc
    15421 ccatgtcgat ccatcatata ggctgctaat aacgcgattc cccacgctcc accttctccg
    15481 gctgtgtcca tcactgtaac tggtgcctcc atagcagatg ctagaatccg ctgtccaact
    15541 tctggtgttt taaaaatccc cccatgcccc actaaacgat caatttggac attctcagat
    15601 ttcaatatat ccattccgat tctcattgca ccaaaagcac tggctaaatg gatccgcatg
    15661 aaattcgcta aatcaaaacg gctacctggt gtacgtacaa ataatggcct tccttcattt
    15721 actcctgtaa tgttttcacc ggaaaaataa ccataagaaa gtaatccccc gcaatccgta
    15781 tcgccttcta gcgctttgtt gaataatacc gtgaataatt tatctctgct tatctccata
    15841 cccaaagaat tggtaaattc ttcaaaaata ctgacccaag cattgatatc cgaagagcag
    15901 ttattcgtat gaaccatgcc tactagttct cctgaaggtg tagtgaccat gtcgatttcc
    15961 ggatggacat tttctagttc tttttctaat acgatcatag cgaaggcgga agtaccagcc
    16021 gaaatgttcc ctgtcccttt ggctacactg ttcgtagcga ccataccggt tcctgcatcc
    16081 ccttctggcg gacaaaccgg gattcctggt ctcaaatttc ccgtagggtc gatcagataa
    16141 gcccctgatt cgcttaactt gccagcttct tctcctgcta ctagaacttt aggcaaaatg
    16201 tgatccaagt caatcgctac gtgcgctttt tcgattaact gaccaaaaat atttttcatt
    16261 tcttgatggt aggttttcgt tgaaggatcg ataggaaaca tgcctgatgc atctcctacg
    16321 cctagtactt tctcaccagt aagctgccaa tggacataac ctgcaagcgt tgtcaaaaaa
    16381 ttaatttctt tcacatgttc ttctttattc aaaatagatt gatacaaatg agcgatactc
    16441 catctctgcg gaatattgta ttgaaatttt tccgttaata ttcgttctgc ttcttcagtg
    16501 attgtatttc gccaagtacg aaatggtact agtaactgtt ccttgctatc aaatgctaga
    16561 taaccgtgca tcattgcact gaacccaatc gacccgattg tcgtaagatt ctcgccatag
    16621 atttcgaata cttctgctgc catttttcgg taactgacgc gcagtccttt ccaaatatct
    16681 tctaatgaat aggtccagat accattttct agtttgtttt cccactcata actaccagcg
    16741 gcaataggag taaaagatga atcaatcaat acagctttga tcctagttga tccaaactca
    16801 atccctaaag atgtacgacc atctatgaca tctaacatac tttgcgcatt tacttccttc
    16861 ataatgattt aactccctcc ttctcgtcaa acctgatcaa accgtctttt tagtagcgct
    16921 tacaatgatt attgtatact tttgtacgta catgtcaagc agttatttac aagaatacaa
    16981 taaaaaacac ttgttttgta cgtacataag ttataaaatg aaattgctta caaaatctga
    17041 tcaaccgcct tctcgaaatt ctagaaggag agaatactta tgaaaaaaga aaaaaaagtt
    17101 tcctcatcgt ttatttattt ttttggcgca tttggcggca ttctatttgg atatgatatt
    17161 ggtgttatga ccggcgcctt acctttttta cagcatgatt ggggtttggc aggtaaagct
    17221 tctttgatag gatggatcac gtcttcggtc atgttaggtg cgatccttgg cggctcttta
    17281 tctggtcttc tttctgataa gcttggacga cgaaaaatga ttcttctttc tgcacttatt
    17341 tttatggctg gttctgtcct ttctgcatct gcccctcaca atggttctta ttttctgatt
    17401 gcagcaagga ttcttctagg tttagctgtt ggcgctgcat ctgccctagt tcctgcttat
    17461 atgtctgaaa tggctcctgc tagactaagg ggaagattgt cagggatcaa tcaagtgatg
    17521 attgcttctg gcatgctcct ttcctatgtt gcagattatt tgctaaaagg gttgcctgaa
    17581 acgatggctt ggcgcgtgat gttagggctc gctgctgtcc ccgcgctgat tttatttttc
    17641 ggtgttttag ctctaccaga gtcaccgcgt tttcttatgc aatctggccg cttggaagag
    17701 gcaaaaagag tattgaacta tattcggaca ccaaaagaag ctgaacagga attcgaacaa
    17761 atccagctga atgtcaaaca agaaaagaca accggaactt cttggcatac tttgttttta
    17821 gaaaaatatc gttccttagt cattgccggt attggtgtag cagtattcca gcaatttcaa
    17881 ggagctaatg ctatttttta ctatattccg ctgatcgtag aaaaagcgac tgggcatgca
    17941 gcgagcgatg cattgatgtg gccgatcatc caaggaatca ttttagttgc tggttctttg
    18001 ctctttttgg ttattgcaga caagttcaat cggcgaactt tattgaaaat aggcggtagc
    18061 gtgatgggac tttctttcat cctaccagct gttttaggaa ctgtactgga tgctcataca
    18121 aattcgttgt tgattctctt gtttttatgt atttatgtcg cattttattc ttgcacatgg
    18181 gctccactga catgggtaat cgtaggcgaa atattcccac tagctgtccg cgggcgagca
    18241 tctggcctag cctcttcttt taattggatt ggttctttcc tagttggctt gctttttcct
    18301 gtgatgactg cttctatgtc tcaagaaatc gttttcggca tttttgggat catctgcttt
    18361 ttaggggttt tatttattca agagatcgta ccagaaactc gtgggaaatc tttagaggaa
    18421 atcgaacaaa gtgcatctaa aaaaacttat cctaaacgta tttctatcca tcattcttaa
    18481 agaggttgtg acagaagtgg tcagcttcaa gaaataagaa ggaaccgctt agtttccagt
    18541 cacaaggaac aatttcaaaa aacagcttct catgtttgat gaatattgtg acttgtgacc
    18601 agttggtagg agaacaccgt ttagtggaga ggtggtgaaa gaaacgttca gccttgagcg
    18661 atgacaaaag ctctcacaaa cattgcgacg agtaaccgta ggagcaataa gaggcggtaa
    18721 aaacccgtct cttatttttc gttttctttc actgtttccc gttcaactag tacaggttca
    18781 tagagaatat ttggctgtgc ctgtcctttt tcaatcgttg tgacgatcca ccgtgcggca
    18841 tcttgtccca acttttcttt tggatgcgtc aatgttgtca attttttatt ccctaaatgg
    18901 cttaaagaag aatcgtctgt tcctacgatc gataggtcat ctggaatatg atagccattt
    18961 tctgtcaact tatctagcat taaactagct atttgatcgt tatagcaaat gatgccatct
    19021 aaagcttgtt cttctttcaa ccgttgtact gttttttcag cgattgtttc tcgtgtttcc
    19081 gtggtgaaag taatgatatc ttccgtcaaa aaacgcacat tatactgttc acacgcgctg
    19141 ataaaacctt tcatcctgta ttttccttga agatcgtcga tttttgtgat gaacaacaga
    19201 cgctgatgtc ctttttttat caaatattct gctgccataa aaccgacttt cacatcatct
    19261 aaacaaatat aagaggcatt taactcttca taagcagcat tgatcatcac catcggaatg
    19321 ccttgttctc tcaaagcaac ataaagtgct aagtttgggt tatactgatt acttttcgta
    19381 ggctcaacga tcaaaccatc taccccataa gcaatcattt tcttcaaaca ctctttctcc
    19441 aattcgtgat cgttattcgt actggctaaa agcaatgaat acccagattc tcgtaattca
    19501 ttttcgattc ctcggataat cgacggaaaa atataatcag agagatatgt ggtgatgaca
    19561 ccgatcgttt ttgttctgtt caatggtgtt ttttctttct tatagctgtc atctacatat
    19621 gtgcccgacc ctttttcttt acgcagatag ccctcattta ccaacacagc gattgcttgc
    19681 cgaatcgtat gtctgctgac gtcatatatt tcttgcagct tgaattctga gggaatggtt
    19741 gtatttactt cgtattcctc acgaaggatc ttttgtttta tctcttctgc aatatcttga
    19801 tattttgcca tttgccttgc ccctttttat ttgtacgtac atatatcata acgagtctgc
    19861 aaggtttatt caagcaaaaa tcaaggtttc ttgtgctcat aaggcatacg gaacaggttc
    19921 accaaattct ggaaatcctt gctcattcca cgtaaagcat tgcgcgttcg cgtgccgatt
    19981 aggattgtct aacggatccc ctttttggtt cttctctggt cgagcatgat agatcaaagt
    20041 atcaaagcac ccgtcttctg aaaccgtaaa cgagttgtga ccaggaccaa attgcttatt
    20101 tttttcagaa gaaacgaata ctggctccga taatttatgc catgattctt ttaataaaag
    20161 attcttgtgc tcatcagccc acagcaggcc catggcatag ttttcatctg ttgcactacc
    20221 agaatacgta atgaatattt tcccattgcg agtaaggact gctggacctt catttactga
    20281 aaaaccccgt ttttcccatt catactcagg tatagaaagg aggatctgct tcccctttaa
    20341 cgtccaagga ttatccattt cggatagata aagattagaa ttcccagtaa tttcaggatc
    20401 ttgttgggcc cacacataat atagctttcc ttgagaagag aaagttgttc cgtctaaact
    20461 gaaagtttcc cttttcgttt ttatttgtcc cttttctatc caatctgtat tcattggatc
    20521 tatatgctga ttttcaagta cgaacatgcg gtgatgatga gattcatcac gtatagtttt
    20581 atgattgctt gcagcaaagt aaatatacca ttttccattg atataatgga cttcaggtgc
    20641 ccagatcaat tcactcatcg gtccttcttc atgagcctgc catacaatca ggcttgcagc
    20701 atgttctaat tcattcagtg actttgccct tcttaattca atcacttggt agcctggaac
    20761 cgatcctgta aaataataat aaccatctgt atgtttatat acccaaggat ctgcccgttg
    20821 ccgaacgata ggattattat aaagtgtctt catcataaca aatttcctca cattttcttt
    20881 attctaatta cattccaaga gtatttttct atccctacat ttaattcgtc atgtttttct
    20941 attgaaaaat agtttatttc aggttttatt ttttctggtt cttcacgtgt atttttgtcc
    21001 aacagatcgc tactggttaa tgtgtagtgc tctccttgat ctagcaaatt gaaatcatgt
    21061 tcaatcgtta tcttttgttg ttcatttgtg cgattgatca agaaaatagt cagctgattg
    21121 ccgttatcta cgatcacact gtccacataa ggaacatttt ttatcttttc acaatcataa
    21181 acagggcaaa tgatttgttg tttcaatact tgacctttag catatttcga taagtgttgc
    21241 atcacataat aagttggatt cctccatgct tgtcctttag catctgtcag tattaaaggt
    21301 attgtgttta ctaacaatga ttgacaacta atctttacgc gatctgcgtg tcgtaaaata
    21361 gccagcccta atgaaccaaa taataatgta tcttccatag taaagtaaca ccaatcgatg
    21421 ggactaccga cttcaaaatc cctatgtctt ttttccggtt gggcatgtac attccattca
    21481 tcaaacgcta gatacattgt tttctcactt ctcaaatatc cttttacata atcacaagta
    21541 gcaacgattt cttcaatttg acgatcgaat cgataagctt ttgcaaggta tgtcgctgta
    21601 tcatcacgct catttttagg aggtttagtc aagtcttctt catcatactt atcaatatag
    21661 ttatgcaaag ctaaataatc tacattctca taagcttctt ctaatgcgat acgatcccat
    21721 tcagggtacg atggtaaacg tggcgtagag cttccaacta agattgtttc aattgaatca
    21781 tccactaact tcatgacttt tgaggcttca tttgctaacc taccatattc ataagcactc
    21841 ttagccccca cttgccatgg accgtctagc tcattcccta aacaccataa tttaacgcca
    21901 aaaggctttt tttgcccatt ttttattctt aaatcactcc agaaggttcc ttttggaaaa
    21961 ttacaatatt ctactaaatt ttttgcttca tcaattcctt ttgttcctaa attcaccgtg
    22021 aatatcggtt tcgcttgtac atgactgatc catttaaaaa attcgtggag tccaaaagaa
    22081 tttggttcta tacctcgcca tgctaaatca attttttttg gtcgttgttc tttcctacct
    22141 acagtatcca cccaatcata accagatgta aagtttcctc caggatagcg aatgacacct
    22201 aattctaatt cctttacata agagaggaca tcttgtctaa acccgtcacc atcagctgtt
    22261 tcatggcccg gttcataaat ccctgtatag acgacacttc ccatatgctc aacaaatgag
    22321 ccaaacagcc gagaactgat ctctcctacc gtaaaatcct ctctgatttt tactttcact
    22381 tcttcgtcca acagctttcc ctacctttca tttcctatag tcaagataat agatacaagt
    22441 ttccatacgc caccagcaaa gataaaaata aaaggaggga aaagaaatat gagcagtaca
    22501 aatcctaata acaatacgca acaaagaata tttctcaaaa aatcaaaaaa tactttccat
    22561 gcactttgtt caaaaatttg taaaaatgtg atatctttgt tttcagaaat cacatatatc
    22621 caggataaaa gaaacatgga ataaataagt aaaactacat atccactaat tgaaagtacg
    22681 attgtctgtt cttctaataa ttgattaaca agaaaataaa taatgatcgt taacagacta
    22741 aggaacaagt tcttaccaaa atgttgcttt attccttgaa agaaaaaata aatgatattt
    22801 cctgatccct cacctttcca ttgcgccatt acataaaaaa cagcacatgt agcaggcacg
    22861 atagtaataa caggcaaaca cacgattacc cagagaatac ctaataagat gtaatcagaa
    22921 accacaatca actgtccata aagtttagtc tcaactattt tcatcttcct cactctttca
    22981 ttccagacat agctgttcct tgaacaaaat atttctgtgc aaaaatataa accactagta
    23041 atggcagcat tgaaataacc gttccagcca ttacaggtcc ataaaaagaa atgttctgcc
    23101 ctgtaaataa agctaaccct gctggtaaag ttctcatttt tgaactactt gtcaatatca
    23161 atggataaag caagtcattc caactattca tcagcgtgaa aatccctaat gataaaagag
    23221 taggtttgct caatggcaac atgattttca aaaaaatatt aaactcactt aatccatcaa
    23281 tgcgtgctgc ctcttccaaa gaatctggta aagtaataaa aaaggaacgc atcatgaaga
    23341 taccaaatgc agttgtcata cgaggaatga tcaaacctgc ataagtatca agcatgccaa
    23401 gcaaattgac ttcaataaat agaggaatca tgaagacttg gaatgggatc atcattgtca
    23461 gcaaaacgaa atagaacaaa actgattttc ctttgaaacg catacgagca aatgcatacc
    23521 ccgcaagaga atcaaagaat acagaagtga tcactgtccc tccagcaaaa atcactgtat
    23581 ttttgatgta atctaataaa gggatcgttg tccaaacctt aatatagtta tctagggtat
    23641 agctatctgc aaatattcta ggcggaaatg agataatgtc tttttcgtgt ttaaaagaag
    23701 aaataatcag ccacaaaaaa ggaaacgcaa taaccaacaa tataactagc atactaatcg
    23761 ttcgaatgat ttttttacca ctaactgctt ttcgactatt cacagtaact gtaatcgagc
    23821 tttctggtgc catacaggtt tccctcctat ttcatttgtt tttctttccg tgacatgtac
    23881 acattagtgg agatagctat agttgcaaca atgataaaca aaaccgaaga cagtgctgat
    23941 gcatagccca attcataagg tgctgtaaat cctcgactgt aaatatattg aacagctgtt
    24001 tctgtcttaa attgtgggcc accagcggtt gcaacatata cttggtcaaa cacttgcatc
    24061 gaaccaatca gattcgttaa aatacaaaaa cctaaggagg gaacgatctg aggtagcgta
    24121 atgttaaaaa actgctgtcg atttgttgca ccatccattt gagcagattc atacaaagag
    24181 ggagagattc cctgaatagc agtcaagaga atcgtcatag taacaccaaa attcttccaa
    24241 acagtcatga tggctaccgt cggcatagca agggttggat cgcgaaaaaa ttgagggatc
    24301 ggcaagccga cttgtgaaaa caagtaagga atcgccccta cattcggatc aagcaacata
    24361 gaaaaaataa tcgcaattga cgtcaatgaa caaataactg gaatgtaaaa tgtcgaacga
    24421 caaaaacgat tgaatctggt atttttagat aatgctactg ctgctaataa accaacgata
    24481 atttgtgttg gcgtttcgag tacggtgaaa tataaagaat gaatcaacga attaactgct
    24541 cgttcatcac taaatatacg tgtgaaattt tcaaaactta caaaatttct tgatgtaaag
    24601 aaaatattca aatctgtaaa actaatagca aatgttccta aaagcggtat gacagtaaat
    24661 aataataaaa ctaaaaaaga tggagttaaa aacaaatacg cagcacgatc aggacgattc
    24721 caatatttgg tgaatccgtt catatagtcc acctcatctt tcattttgat aaaatataca
    24781 atcttggttt aaacgaatct gaataagata tccgaacaat cgctgtgatt tttgcaataa
    24841 cacctatctg caaatactag gtcgatcatt cagatatctg tatcttgcca acagtctatc
    24901 ctgtataaag ttctgtacta tttttgcagt aattgattga tggcatcatc tgcatcgttc
    24961 atgagtgtct caggatcttc tcctgctaat aatttttcaa tcagtggatt gatgatatcg
    25021 ttattgatcg cagagatttg ggtaaaacct ggtaaatatg gctctgcata gtccatttgt
    25081 ttcgataatt cagatacaat aggatcattt tttacttctt catcttttgc tactgaatcc
    25141 aagtaggcag gaaaaccatt ttcgatactc cattttttac caatttcagt tgtattccaa
    25201 tatttcaaaa actcatagac agcttctttt ttattattat ctgtacttgc cggaatggca
    25261 tatcctacgc catctaaaat agctgttttt ttgtctgaat cactcccttg tggtaaggta
    25321 gtcacattat aatcaatctc gtttgctttc aatccgttat ttaaccatgg cccattgatt
    25381 tcaatggcta gttgaccagc attcattaaa ttgtccattt cggcgccaga aatcgattct
    25441 ggaccagtct tgtctacatg gatcattttt tggatcgctt ttaacgtttt aaggttctca
    25501 ggagacgcaa attcagactt agtataatca tcgttcacta atttaccgcc atttgctaac
    25561 atccagttat ataacggtgc agttccatcc ttattgaaga caaaacctga tacattttta
    25621 ctactatcag ttaattttgg tgccatttct gtcaattctt cccaagtagt aggtggttgt
    25681 tcagtatcta atccagcttg tttaaacaaa tcattattcc aatacatata catgccttgt
    25741 acctgcatgg ggacaaaata ctgtttacct tctacttcac ctaattcaat agccgtttct
    25801 ttaaaatcag atttatctac tccatcatat tcccagaaat cgtctaatgg ttgtaccgct
    25861 ccgttttcta catactgtgc aaaatctgga taatttaatg caataaagtc aggtgctttt
    25921 ttagaagaaa tcgctgtagg aagtttttca ttcaaattgg cccaagtcat gatatccatt
    25981 tcaattttga tgtttttatc atttgtttta ttaaattcat taacgatttc ttgcaatatt
    26041 tcaccatcag atgcagtaaa cccattccaa aaagtcaaag tcacttcttt atcagaatca
    26101 gattctgctg aacttccgcc accgcaagct cctaaaatac ctgttgcagc taccaacaaa
    26161 gagccaatta accatttttt acctttcatt ttttgttcct ccttcatctg ataaacgaca
    26221 tctgaccaca ttccaagata gcggtttcaa taaacaattg attttcccat cttcagcttt
    26281 tacatttgtt gaatgacttg gttttacttg ttcactttta ggaagattag ttgcttttgt
    26341 atcaaatcca ctcatttcag aaaaatcaat cactccctct aatgataatt cagacaagtg
    26401 aacagttaga ttcatttctt cttctccacg gttaactgca aatatgacaa gttctttgtc
    26461 atttattacg gctatacttt ctaaatacgg cacttcttca aaatcttccg tcgcatagtt
    26521 tgctgaacga atttgtgggc ttaacacctc tccttgtcca tacacagcag cttgcataaa
    26581 tggataatag atcgtctgtc tccaagcttc tccgtttgtt tctgtcctga ttggtgcaat
    26641 aacatttacc aattgagcaa gacaagcaat ctttactcta tctgcatgtt tcaataaagt
    26701 gattaataga cagcctaata gtaatgcatc ttcaaaatta tagttatctt ctagtaaagg
    26761 aggagcaatc tgccaaggcg ctgcttgctc atcttgttcc gctgaatgat accaaatatt
    26821 ccattcatca aaggacaggt tgatctgctt cttactatgt ttcttcgcct tgactgtatc
    26881 gcacatcgct gtcacttcag aaatgaaccg atccatgtct aaggaacgtg caagataatg
    26941 agaaagatca tttttttggt tgccataata ttgatgcaga gacaaataat ctatttgatc
    27001 gtacgcttga tcaagaactg ttaactccca atcaccaaat gtaggcattt gccgattcga
    27061 acttccgcat aaaaccacct caattgaatc atctacgagt ttcatcgcct tagccgtttc
    27121 ctctgctaac cgtccatatt cttccgctgt tttatgtcca atctgccaag gaccgtccat
    27181 ttcattccct aaacaccatg tcttaattcc aaaagggctc tcttgaccat tcgaacgcct
    27241 taaatcactc caataagttc ctttaggaaa attacaatat tcgactaaat tacgagctgc
    27301 atctactcca cgagtaccca gattgacagc catattgatt tcggccccca catgctgaca
    27361 ccagttataa aattcatgaa tcccaacttc attcgtttca attgtccgcc aagctaaatc
    27421 caaccttcta ggtctatttg ctatgggccc aatactgtct tcccaacgaa atcctgacac
    27481 aaaatttcca ccaggatatc taatcatggg aatgttcaat tcttttacca agtgaatgac
    27541 gtcttggcga aatccacttt tattagctga tggatgatct ggctgataaa tcccttcata
    27601 tactgctcgt cccatatgtt caataaatga actgtacaac cgcggatcaa ctttgcctac
    27661 acgcttgaca ctactaatca acattgtggc ttccataact gacctccttt aatttataca
    27721 tgaataatac acctcgttga aagcgttgtc aataatataa atgttaaact tacttatttt
    27781 ttatcgattt taattaacca aaatattaat aatgattaaa acaaataatt taatataact
    27841 tttattaaca aatatgttaa tatataagaa aaacctttta agaggtgata tatatgcaac
    27901 tacaaataaa cgaagaatcc ttaccggtat atgaagcatt agctagtaaa acaaggatca
    27961 agatcatcca gctactttcc aaaaaaaaga tgaatgtcaa agatttggca aaagaacttg
    28021 gagtgagcag tgctatcacg acgatgcacg tgaaaaagct agaggaagca aacatcatca
    28081 aaacagaaaa agtaggacaa caaaaaattt cgagccttcg tgtcgataaa atcgatatca
    28141 gttttccaga aaaaatattc aatgcttttg atacgaaaga aacatcgata cctattggtc
    28201 actatacaaa ttatgcaatt gaaccaacat gcggtttagc tacgatccat gattttatcg
    28261 ggaaagtgga tgaaccacgc tattttatgg atcctcgaag gatggatgcc cgcattctct
    28321 ggtttaccag cggatttgtc gagtatcagg cacctaattt tttaaataca gaggatacat
    28381 tggagatgtt ggaagtttct gtggaaattt cttctgaatt tcctttttca aatgacaatt
    28441 ggccttcaga tatcactttc agcctaaatg gtgtggagct aggaacatgg acgagccctg
    28501 gagattttgc tgatatccgc ggtaaatata cgcctgattg gtatccagat aatcttaatc
    28561 aatacggctt gctgaagacg atccgaatta ccaaacactt gacgaatatg aatggagaac
    28621 ccttgtcaaa catcacgatc aacgacatcc ctaaagagca agacacttgg catttgcgaa
    28681 tcgaagtgaa agacgatgcc aaacatgtgg ggggctgtac tttatttggt aaaggattcg
    28741 gtaattacga tcaggatatc aaattaaaag tttactacag ctgatatttt tcttttctat
    28801 tttataaaac aaaaacagcc aatccctatg aagggacggc tgtttttgct ttattgttat
    28861 ttccagaaat catccaaaac ggaaattggc aaatgtcgtt tatgttgtgt cttgttccac
    28921 catgcttcga ttttttcttg tgcttgagcg ggaacttctt ttccttctag ataatcatca
    28981 atttcattat atgtgacacc tagtgcgact tcatctgcaa tcattggttt cccatcttca
    29041 agatctgcag tcggaatttt tgtgtataat ttttccggtg cattcaatgc ttgaagcaat
    29101 tgtttccctt gtcgtttgtc caaacggaat aatggcaaaa tgtcggctcc accatcacca
    29161 aatttcgtga agaatccagt aatgttttct gcagcatgat ctgtccctaa tacggctcct
    29221 gctctttctc cagctacggc atattgcgta atcatacgtt gacgtgcttt gatatttcct
    29281 ttgttaaaat cgctgattcc cacacctgct tcgcttaata cttgtacttg tgcatcaaca
    29341 gctggtttga tatttactcg cagactaaca tcgggctgga taaagtcaag agcagctttt
    29401 gcatcttctt catctgcttg ttcaccataa ggaagacgaa cagcgataaa ttgatagttt
    29461 tcatcttttg tttcttcacg catttcttcc atcgttagtt gcgccaaacg gcctgctaaa
    29521 gtagaatctt ggccgccgct gatacctagt acaaaagttt taagaaacgg atattttttc
    29581 aaatatgctt tcataaagtc aatactttta cgtatttctt cttttggatc aattgttggt
    29641 ttgacaccta attcattgat aatttctgtc tgcaggctca tttattctcc tcctatgctt
    29701 ctgtatgaag ttctgctacg ctttttttca ttcgttccat gatagccatt ttatggttcc
    29761 agcattcagt agacagatcg actgggtatt tttgcggatt caagtctcgt ttatactctt
    29821 cccatagtga atctagattt tcttttgtat attctttgat ttcgtttagt gcaggtaatt
    29881 cgtaaactct ttttccttcg ataaagatat cttgtaaaac tgggcgagct gtaaagtctc
    29941 gaacagtttt attgataaac gtatgcactg gatggaacat gaagatagct tcttcttctc
    30001 gagggtcttc atcccataat gtcacgtagt ctccttcaga tttcccatca aaatttcgag
    30061 tgatgcgcca cacttgtttt ttccctggag tagttacttt ttctgcattg cttgataact
    30121 tgatcgtatc catcattttt ccttcatcat cttcgatcga tactagcttg aatactgcac
    30181 caagggctgg ctgatcatag gctgtgatca attttgtccc aacaccccat acatcaattt
    30241 tagctttttg catctttaaa ttcaaaatcg ttgcttcatc caagtcattc gaagcataga
    30301 tttttgcttc cgtaaatccg gctgcatcta attgttcccg aacacgctta gatatatagg
    30361 ccatatctcc actgtcgatg cgtacgcctt ggaagttgat tttgtcacct aattctttcg
    30421 ccacgcgaat cgcacttggc acacctgatt tcaacgtatc ataagtgtcg actaagaaca
    30481 cgcagtcttt atgggtttta gcgtaggcca taaatgcttc ataatcattt ccataagatt
    30541 ggactaacga atgagcatgg gtcccgctag ctggtatccc aaatatcttc ccagcacgta
    30601 cattgcttgt tgcatctgct ccgccgatat atgccgctct agttccccag actgctgcat
    30661 ccagttcttg tgcccgtcta gttccaaatt ccaatagtgg atcttctccg atgacagatt
    30721 tgatacgagc tgcttttgtc gcgatcaacg tttggaaatt gaccatattc aacagtgctg
    30781 tttctactaa ttggcagtgc gctaaaggcc cttccacttg gattaatggt tcatttgcaa
    30841 aaactagttc tccttcacga gcagaacgaa ctgttgcttt aaattcaaaa ttttgtaaat
    30901 actccaaaaa accttcagga tagacttcta atccacgcaa gtaagcaata tcggaatcat
    30961 tgaacgtcag attctctaaa taattgacta accgttccaa tcctgcaaaa accgcgtatc
    31021 cgtggttaaa aggcatttct cgaaagtaac attcaaaaac agcatgtaaa tcagctcgac
    31081 ctaattccca gtatgtctgc atcatattga tttgatatag atccgtatgc agcgtcaaac
    31141 tgtcgtctgg gtaagtatta ttcatcttgt attactcctc ttggcaagtt ttcatttcct
    31201 acccattata acaaaaaaaa ttaataggaa aagaaatctt ccattttttc tgaaaaagca
    31261 catgttttac ggatatttct tgctaaaaat gaactccatc tgctaaaact atcctttctt
    31321 attggtattt aacctaaacg agaaagaata cttattttat tcaatgtatc cgagacacga
    31381 agaaattagc gttttctatt tttcttactt gataaaataa gtagattttc ctactacaat
    31441 cgtagccgtt aagataccga tgattgaaca aatataacca aatgtctcta caacataatc
    31501 aatggcaaaa gtttttgaag caataatacg tctttccttt tttgcttctt atccccaaag
    31561 aaacggatat aacttattat gtattgaatt tttttggaaa ggcgaattat gacaagatat
    31621 accttgcgtt gaaaaaactc gcggaaaaaa tatcacacgt ttttaaaaag atacaaagta
    31681 agaaagcagc tgtcaataga atggaaacaa aaatggcgag acatcaatca gtctcgcctt
    31741 gttttttatt gtatacatta gatatggagc aggttaccat aaagatgatt attagtcgtt
    31801 attaaataga atccttcgta tttttttgtt ctatcactac aatatcttta tttggacgtt
    31861 tgaattgtat tccggcacgt tctagctcct gtttcactcc tacatggact ttgacatctt
    31921 ctcgtgtcat ttttaaaacg tgatgaatcg catagtcgat ttcttgcctt gtgatttttt
    31981 tcttcgtatt gatcatcacg acttgctttt tatcatatgt gtctaccaat agcagttgtg
    32041 ttcgatctaa cgaaaataat ttgtaataat taatagccgt attcccaaaa aagaattcca
    32101 ctactttagg ataaaatgtc ttaatattta aattactatt cacaggtgtt tcgattagct
    32161 tcatctcatt cctcttttct atatagtcga tttagatcca atcttaagtt atgttaccat
    32221 ctttcctagc attaaggaat cggttacaat tgaaccttta aagacaaaag gatttacctt
    32281 ttatcactta atagtataac ataatttcgt gagaaaaaaa tacaattttt tcttgaccat
    32341 ctcgttacgt aaaggattat gcttacattt gtcaggagga aaaataatgg agtacacaat
    32401 aaagaaaatg tcagagatat caggagtcag tccgagaacc ttgcgctttt atgatgagat
    32461 tggactgcta aaaccagcta ggatcaattc ttcaggttat cggatttatg gcaaaaaaga
    32521 agtcgatcgt ctgcaacaca tcttatttta tcgaacaatg gcattcaagt tggatgatat
    32581 ccaagaggtc ttagacaatc catcttttga ccaccagaaa gctttaatca agcatcagca
    32641 aatgttgctg gaaaaacggg cacaaatcga taccctcttg acaactgtcc aacagacact
    32701 tgatatgtac gaaggaggaa gaaaaatgag tgacaaagaa aaatttgaag ggtttaaaca
    32761 acaaaagttg aaagaaaatg aagaatccta tggacaagag attcgacaaa aatacggaaa
    32821 aaaaaaggtt acagcatcca atgaaaaatg gatgaatatg gataaagaaa cgtatgaaga
    32881 aatgcaaact gttgaaaagc agatgttatt tgatctcact ctttacttaa aagaaccaga
    32941 agatgaaagc ttagcagacc gtatcttcca agctcataag aagtggttct cttacagctg
    33001 gcctgaatat cagccaaaag cgcataaagg actaggaatg atgtacgaag ccgatgaacg
    33061 gttcactgca tattatgacg agcgaagtgg tcatggtgca gcaaaagctt taaatgaaat
    33121 tatccaaaaa aaggcgtaat cttttacatg atccaacaaa aaagtacacg taaaatgacc
    33181 taataacaaa acagcagaca cccaagttag atttttatgc taacttttgg tgtctgctcc
    33241 attctttcac aagttttttc atcctacttt aaatatattc atcgttgaat aaacgtataa
    33301 ggtattttca ctttatcctt tttttctgta agaaacaatt gaaatgccat ttctccgcaa
    33361 gcttccagat ggtggtcgat ggttgggaaa ccaagaagct cgctgatcag caaattatcg
    33421 cgtccaatca ccactggtgt cttatcttgc agatagcttt gcaagatgcc tgctgcaacc
    33481 atatccgaat ttgtaaagac accatcaact ccaagctctt taaaaaagaa gcctgcttgt
    33541 tttcctttct ctgcatctat acaatcccaa aaaacaagtg attcatcaaa agatggaaaa
    33601 agttgatgac atagctgcaa agtgattttt gagttataac ttagcctgcc acttcgcccc
    33661 aacgtgatgc ccaattgctt tataccttgc tgcttcaaat aagtaagacc ttctgtaatt
    33721 gattgttctc gatcaataaa gacacagctt gcctgttttc ccttgatttc ttcacaaaaa
    33781 actagaggtc catatttttg atatgccaat aaatcatcta gtgtattggc acgagacgta
    33841 atgatcaatc catcgaaagc tttagctgca aattcttcaa gataccttcg ctctttttct
    33901 ttatgatagc ccgtcggtaa aagtgtcact ttgtaatcat aatcaaacgc tgcttcaatg
    33961 atcccactga tcaactgatc aaaatacgga tgattggtat aaggcagaat cactccaatg
    34021 cttttcgttt ttccataact taactgtctg gcatttgcat tagggatata atcgacttct
    34081 ttcatgaccg caagtatttc tttccttttt tcctcatcta catatggatg tccattgatt
    34141 acacgtgaaa tagtagaaac tgaataacct gtgagctttg cgatatctcg aatgtttgcc
    34201 actgctgctt cgccacctat acaaaaagat tttcttgtaa atagtctacc acagctggac
    34261 cgcattcaaa aaactgatac atttttcctt catgcgaaaa actgatccgc tgcccgcccc
    34321 aatatatatc ttttgtcaaa ttttcgattt cattcattgg tatttctttt actatattcc
    34381 tattcaaccc ttgaaaactg tacaacgtta atacggatcc cttgaactcg acaaacgcat
    34441 aaaaataatt gttcgttgtg ccatctgtta tctctacata agtattcatt gcaagccctc
    34501 cttttgatac tttgagtata gtgtatgaaa cgcgtctcat agcaaggagt ttgttttgcc
    34561 aaagaaaagt tttattcctc tttcattatt accaaagtct cttattttaa attgaataca
    34621 ttataatgat actgtaaata tatctttatt attagtgaat
//
LOCUS       DACGLW010000020        34484 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.020, whole genome shotgun sequence.
ACCESSION   DACGLW010000020 DACGLW010000000
VERSION     DACGLW010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34484
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.020"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            387..1076
                     /locus_tag="IXT53_001041"
     CDS             387..1076
                     /locus_tag="IXT53_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ4904885.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1063..2271
                     /locus_tag="IXT53_001042"
     CDS             1063..2271
                     /locus_tag="IXT53_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ4904886.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2565..3836
                     /gene="serS"
                     /locus_tag="IXT53_001043"
     CDS             2565..3836
                     /gene="serS"
                     /locus_tag="IXT53_001043"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HAQ4904887.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4385..5869)
                     /gene="guaB"
                     /locus_tag="IXT53_001044"
     CDS             complement(4385..5869)
                     /gene="guaB"
                     /locus_tag="IXT53_001044"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HAQ4904888.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6374..7069)
                     /locus_tag="IXT53_001045"
     CDS             complement(6374..7069)
                     /locus_tag="IXT53_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HAQ4904889.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7159..8259)
                     /gene="ychF"
                     /locus_tag="IXT53_001046"
     CDS             complement(7159..8259)
                     /gene="ychF"
                     /locus_tag="IXT53_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HAQ4904890.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8268..8459)
                     /locus_tag="IXT53_001047"
     CDS             complement(8268..8459)
                     /locus_tag="IXT53_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HAQ4904891.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8480..9370)
                     /locus_tag="IXT53_001048"
     CDS             complement(8480..9370)
                     /locus_tag="IXT53_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HAQ4904892.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9357..10124)
                     /locus_tag="IXT53_001049"
     CDS             complement(9357..10124)
                     /locus_tag="IXT53_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ4904893.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10136..10849)
                     /gene="rsmG"
                     /locus_tag="IXT53_001050"
     CDS             complement(10136..10849)
                     /gene="rsmG"
                     /locus_tag="IXT53_001050"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HAQ4904894.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11081..12982)
                     /gene="mnmG"
                     /locus_tag="IXT53_001051"
     CDS             complement(11081..12982)
                     /gene="mnmG"
                     /locus_tag="IXT53_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HAQ4904895.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(12999..14396)
                     /gene="mnmE"
                     /locus_tag="IXT53_001052"
     CDS             complement(12999..14396)
                     /gene="mnmE"
                     /locus_tag="IXT53_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HAQ4904896.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14740..15078
                     /locus_tag="IXT53_001053"
     CDS             14740..15078
                     /locus_tag="IXT53_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4904897.1"
                     /translation="MSPFRSAALEEESDPRINDVVLSTGNINQKYIVRDIVFVVECLE
                     ADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQKKRDGKYFLELFAYGT
                     VIQFIQTTIG"
     gene            complement(15246..16004)
                     /locus_tag="IXT53_001054"
     CDS             complement(15246..16004)
                     /locus_tag="IXT53_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAQ4904898.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16022..16840)
                     /locus_tag="IXT53_001055"
     CDS             complement(16022..16840)
                     /locus_tag="IXT53_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAQ4904899.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(16837..17193)
                     /gene="rnpA"
                     /locus_tag="IXT53_001056"
     CDS             complement(16837..17193)
                     /gene="rnpA"
                     /locus_tag="IXT53_001056"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HAQ4904900.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17311..17445)
                     /gene="rpmH"
                     /locus_tag="IXT53_001057"
     CDS             complement(17311..17445)
                     /gene="rpmH"
                     /locus_tag="IXT53_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HAQ4904901.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            17969..19303
                     /gene="dnaA"
                     /locus_tag="IXT53_001058"
     CDS             17969..19303
                     /gene="dnaA"
                     /locus_tag="IXT53_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HAQ4904902.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDIKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19501..20631
                     /gene="dnaN"
                     /locus_tag="IXT53_001059"
     CDS             19501..20631
                     /gene="dnaN"
                     /locus_tag="IXT53_001059"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HAQ4904903.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            20860..21102
                     /gene="yaaA"
                     /locus_tag="IXT53_001060"
     CDS             20860..21102
                     /gene="yaaA"
                     /locus_tag="IXT53_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HAQ4904904.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21089..22213
                     /gene="recF"
                     /locus_tag="IXT53_001061"
     CDS             21089..22213
                     /gene="recF"
                     /locus_tag="IXT53_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HAQ4904905.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDSRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22210..24156
                     /gene="gyrB"
                     /locus_tag="IXT53_001062"
     CDS             22210..24156
                     /gene="gyrB"
                     /locus_tag="IXT53_001062"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HAQ4904906.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24176..26647
                     /gene="gyrA"
                     /locus_tag="IXT53_001063"
     CDS             24176..26647
                     /gene="gyrA"
                     /locus_tag="IXT53_001063"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HAQ4904907.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            26883..27182
                     /locus_tag="IXT53_001064"
     CDS             26883..27182
                     /locus_tag="IXT53_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HAQ4904908.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27232..27744
                     /gene="ssb"
                     /locus_tag="IXT53_001065"
     CDS             27232..27744
                     /gene="ssb"
                     /locus_tag="IXT53_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ4904909.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27770..28006
                     /gene="rpsR"
                     /locus_tag="IXT53_001066"
     CDS             27770..28006
                     /gene="rpsR"
                     /locus_tag="IXT53_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HAQ4904910.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28149..30122
                     /locus_tag="IXT53_001067"
     CDS             28149..30122
                     /locus_tag="IXT53_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HAQ4904911.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30128..30580
                     /gene="rplI"
                     /locus_tag="IXT53_001068"
     CDS             30128..30580
                     /gene="rplI"
                     /locus_tag="IXT53_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HAQ4904912.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            30854..32221
                     /gene="dnaB"
                     /locus_tag="IXT53_001069"
     CDS             30854..32221
                     /gene="dnaB"
                     /locus_tag="IXT53_001069"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HAQ4904913.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            33001..34293
                     /locus_tag="IXT53_001070"
     CDS             33001..34293
                     /locus_tag="IXT53_001070"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HAQ4904914.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT        10512 a         6703 c         6827 g        10442 t
ORIGIN      
        1 ttctttttta acgactcagt tatcttatca tgtcatttcc aacatgtcaa caacttattt
       61 tttattattt aataagttat tggtgttctt ttgaacaaat aataatatat caacatcaca
      121 tgtgaaagtc aataagaaaa tgcttattta tcaacatttc tagcttacat gatcattggt
      181 tagttctggt cctttttcgt tcgtctttat aactccgaga gacgtaaaat ccttttgttt
      241 tcagtttaat gttcatgttc ttgtcttatt cacaccaact agatgtctca ccttctaaaa
      301 ttttccttaa caaagtgctg cttttatcta tttctgcttt tttttgctag aataatagta
      361 acaatatgac tctggaggat ttatttatga aaattttgat tgctgacgat gataaagaaa
      421 tcgttgaatt attaagtatc tatgttcaca atgaggggta tgaagcagta aaggcttatg
      481 atgggaaaga agcattatct aaaatcagaa caatccctga tattgaatta cttattttag
      541 atatcatgat gcctgagatg gatgggatgc aagtagtgaa agagctacga aaagaatccc
      601 aaataccaat cattatgctg acagcaaaaa ctactgatat ggataagatc aaaggactgg
      661 tcgctggtgc agatgattat gtgaccaagc catttaatcc gttagaagta atggctagag
      721 taaagtcttt attacggcgt acaaaaatgc agattgcccc agatcagcct gatgtattag
      781 aagtaaatgc attaatcatc aataaagact ctcatgaagt taaaacaatc gatgggaaag
      841 aaatccagtt aacagctttg gaattcggta tcctctactt gcttgctagt catcctaatc
      901 gagtatttag cgcggatgaa atttttgagc gtgtttggaa acaagaaagt attgtctctg
      961 ctaaaactgt catggttcac gttagtcatt taagagataa gattgaagaa gcaactggtg
     1021 gtgaaaaagt cattcaaacc gtttggggag tgggctataa aattgacgca agatagtaaa
     1081 cgagcaactg aaaaacgccg tcggatcaat ttgacttcaa aagaaatcag cgaattgtta
     1141 gcggaaggaa tcatcacgat tatccttttg cttctattaa atgtttctat tctggttgtt
     1201 atcagttcgg tgatcaacag ttcaccttct ttgacgaatg cgatttggga ttcaaagaat
     1261 atttttgcag aacgattgaa cactgatttg ttctggaacg gccggaattt tattattccc
     1321 tttttctttc tattagatat tggtgttttg tattggcgct tgattcgaag atatcgccaa
     1381 atgcaattgc ggcatatcat cagcgaactt cactacatcg caaacggaaa ttatgatcat
     1441 cgtatccctt ttgaactgag tggtgactta agccgagtgg taacaagtat caatggattg
     1501 gtggatagta ccgtagcagc gattgaagac gaacgtaaaa tcgaaaaatc aaaagatgaa
     1561 ttgatcacga atgtcagtca tgatatccgt accccactca cgtccatcat tggataccta
     1621 ggactgatag aagatggcca gtatcactca gaagaagatc ttttaaagta tacgcatact
     1681 gcttatatca aagcaaagca gatgaaatct ttagtggacg acttatttga atacactaag
     1741 gtacgccagc ctgctgtccc ggtgaatttt tctgcctttg acatgatcca gttgatcgaa
     1801 cagttagcag ctgattttga gttggaagct tctaaaaaaa atatccaaat acttgttcaa
     1861 tcaaaagtag actcccttat catggatggc gataccgaaa aacttgtacg tgtcttcaat
     1921 aatcttttga cgaatgcgtt gaaatacgga aaaggagcaa cgaagatcgt aatcgaagta
     1981 gaacgtatcg gttcagaagt tgttgcaaca gtcaaaaata acggagcaat gatcccacaa
     2041 caggcaatcg ataatctatt cgaccgtttt tatcgtgtag aagaatctcg ttcacaagca
     2101 actggaggaa ctggacttgg tttagcaatc gctcaaagca ttgtggcttt gcacggcgga
     2161 tacatttatg caaaatcaga taagcaatgg acttctttca tcatgcacct tccaatcaag
     2221 aaaaatgaaa aattacctat tgatacacag gaagttattg acgaaagctg aaatattcgc
     2281 tattcttatt acaattatta gaataaatga aaaacacaga acccagtaca ctttattctt
     2341 tttcagagag ctgatggcag gtgtaaatca gcaaaggata atttggaagt tccagttcgt
     2401 ttacgtgccc tttcgaaata agaaaggtcg gttcaacagc cgttatgtgt tcaacaagtg
     2461 cagctgacgc tgaacttggg tggtaccgcg aatttcagac tgattcgtcc caagaataat
     2521 gggatggatc agtctttttt tattttcaaa aaggaggaaa tcatatgtta gatgtaaaaa
     2581 tgatgcgaca aaatattgaa gaagtacaag aaaaattgat gactcggggc gtaaaaaaag
     2641 aaacattagc tgaattcatg gaactagatg aaaatcgtcg taagctacta gtcaaaagtg
     2701 aagaattaaa aaaatatcga aatgatgttt ctgctgagat cgcccaattg aagcgtgcaa
     2761 aagaagatgc ttctgctaaa atcgctgaaa tgaaagaagt cggtggcaac atcaaagcat
     2821 tggatactga aattgctgaa gtggatgaaa aattgaaaca aattgcaact actttgccaa
     2881 accttcccca tgattctgtg ccagttggaa cagatgaaga ggacaatgta gaaatccgtc
     2941 gttggagcga accacgtaca tttgcttttg aaccaaaacc acactgggaa attgcagaaa
     3001 atcttgatat tttagatttt gaacgtggcg caaaagtagc cggtagccgt tttgtctatt
     3061 acaaaggatt aggtgctcga ttagaacgtg ctgtctacaa cttcatgctt gatcagcata
     3121 tttacgaaca cggctatact gaaatgatca ctccttatat cgtcaacagt aaagcgatgt
     3181 ttggtacggg acaatttcca aaattcaaag aagatgtttt ccaattggag aatactgatc
     3241 taacattaat cccaacagct gaagtaccat taactaacta ttataatgat gaaattttag
     3301 atggtgcaga tttacctatt tatttcactg cactaagccc tgcttttcgt tctgaagccg
     3361 gaagtgctgg tcgggataca cgaggactga ttcgtcttca ccaattcaat aaagtagaaa
     3421 tggtcaaatt ttcggatgta gagcattctt acgaagaatt ggaaaaaatg acagctgatg
     3481 cagaagatat cttgaaaaaa ttgcatttgc cttatcgtgt gatggcctta tcaactggag
     3541 acatgggctt ttctgcagct aaaacttatg acttagaagt atggatacct gcacaagata
     3601 cttatcgtga aatcagttct tgttctaact gtgaagattt ccaagcacgc cgcgcaatga
     3661 tccgttatcg cgataatgat ggaaaaatcc actatactca tactctgaat ggttcaggac
     3721 ttgcagttgg ccgtacagta gctgccattt tggaaaacta ccaaaatgaa gatggttctg
     3781 tcacagttcc agaagcgctc gttccttata tggggaatct gaaagttatt aaataaagag
     3841 gctgtgaaaa agccgcttag tttcgagtat taagaaaaat tttgtgaaaa tggctttcca
     3901 catttttgca taaattggac ttaataccga gaaactggct tttgaacacc atttatcgag
     3961 aggttgtgaa atcagcgaat taacctgagt attagagaaa aaaatggaaa aatagtccct
     4021 catattttac cgtttgttga actaataccg agggtcagct gattgaacac cgtttagtgg
     4081 agaggtcgta agcctacccc ccagctccta gttacaagga caaattcaaa aaacagctct
     4141 tcatatttta tgaatgttgt acttgtgatt cggaggtggc aagggaacac tgtttattca
     4201 aaaagaggct gggacagaag cgtttaactc caagaaataa gaagaaattc acgaaaattg
     4261 cttttcaaat ttttgtgaat ttcttcttat ttccgaagga gttgcttcta cttccgccgt
     4321 ttatacgtgt ttagagcgtg agacaaaagt gttctttact tttgtcccac gctctttaat
     4381 gtttttaaga ttcaactgaa tagtttggtg cttcttttgt gatttgtaca tcatgagggt
     4441 gtgattcttt taatccactg ccgctcattt ggataaattg cgcttcatca cgtaattgct
     4501 gtagattggc tgccccaaca taacccattc ctgaacgcaa accgccgatc atttggaaaa
     4561 taatatctgc aacacttcct ttataagcaa cccgaccttc gattccttct ggtactaatt
     4621 tatttgcttc attgacacta ccttggaagt aacggtcttt tgaacctttt tccatcgcgc
     4681 ctaatgagcc catacctcgg tatgttttga atcgacgtcc ttggaagatt tcgaattcac
     4741 ctggtgattc atctgtccct gccaacatac ttcctagcat cactgcatgt ccgccggcag
     4801 ctaatgcttt gacgatgtca ccagaatatt tgatccctcc gtctgcaata atcgctttgc
     4861 catattgacg agcaactgat gctgcatcat aaatagctgt gagctgaggt acacctacac
     4921 cggctaccac acgtgtcgta caaatcgatc ctggtccgat accaactttt acgacatcta
     4981 cacctgcatc atataaagct tttgtcgctt cagccgtcgc aacgtttcct gcaatcaatg
     5041 ttgcatctgc aaaagttgaa cggatttctt gaatttttcg gatcacacct gcactatgtc
     5101 catgagctgt atcgatgatg attgcatctg cccctgcttc taataaagca ttcgcacgct
     5161 caaatgtatc gcttgttaca ccaacggctg ctgctacaag caatcgacca tgttcatctt
     5221 ttgctgcatt tggaaattcg atcactttct cgatgtcttt gatcgtgatc aatccgctta
     5281 aacgaccttc gttatctacg attggtaatt tttcgatttt atgtttttgc aaaatctttt
     5341 ctgcatcttt tagagaggta cctactgggg cagtcactaa gttgtctttt gtcatcacat
     5401 cagcaatcgg catgtgataa tctgttacaa aacgcatgtc gcggtttgta attatcccta
     5461 ccaattttct gttttcaagt gtttcaacga ttggcacacc actgatacga tatttgctca
     5521 tcaattcttc tgcgtctgct acaagatgct gcggcgttaa gaagaacgga tcaatgatta
     5581 cgccactttc agaacgtttg acttttctta cttcatccgc ttgctggctg atcgtcatgt
     5641 ttttatggat cacacctagt ccgccttgtc ttgccatagc aatggccatc ttgctgtcgg
     5701 taacagtatc catacttgca ctgatgattg gaatattcaa cttaatattt ttagctaatt
     5761 gtacgctcat atccacttcg tttggtaaca cgtgactttc tgctggtatc aataagacat
     5821 catcaaaagt aaatcctttt ttcgtgaact ttgtttccca gttggacatt tttagtacca
     5881 ctcctctaat ttagtttatc tattaaaata attgattttt tcatttaagt caatgatcta
     5941 cttggaatca agttagcttt ctcatagaat accaatataa agcgtttaca tctgtgtgta
     6001 ttaaaagaga ttgtaaaaaa gctgtcctgc atcaagtaat aagaacagcc aatcaaaaac
     6061 agcttctcat gtttttgaat tggttgggtt tattaccgca gatgcaaact tttgaacacc
     6121 gtttagtgga gaggctgtga aaaagctgct tagtttcgag tagtaagaaa aattttgtga
     6181 aaatggcttt ccacattttt gcataaattg gacttaatac cgagaaactg gcttttgaac
     6241 accgtttatc gtaaaaaggg actgtgacaa gacttttgtc acagtccctt actattattt
     6301 aactatggat aaacaaaatg aggttcttta ctacccctag atatctagca aaatagctga
     6361 tctttttgct agtttatctt cttgggacgg ataaacttcc aataatcccg tattttttct
     6421 tcaagtagta acggattgct aaagcagttg ccccgataat gataactact actggatcta
     6481 atactggatt gataactggt ggcaatagtg ttgtcgcaga aaacagtacg acccaaagaa
     6541 gtgtcgttcc acctaggata agcaatgtct tacccattcc tggtttcttg cttcgatctg
     6601 ctccgggata ttcatattga tagatgtatt tgtacatcat ataaaagacc cagccaccga
     6661 ttgcagaacc tagaacgagt gtagaaaagc cataagctgg cgttgttcca cgtgaaaaca
     6721 atccggtaat cgcaatcatg atacttaata gaccaagcaa cagcaacgta ttatctaacc
     6781 acatcaataa aggggtggat gaatttttta cttctgctgg cttattgatg attgcttccg
     6841 ttctttcaga gactgtccca aacaattgac gggctgttct tccgcctttt tgttctgcaa
     6901 ctaaagtagg cagaatgtca tgaagggctt ttactttttc ttcttcagac aaattcgctg
     6961 cttctaaaga ttttttcaaa tcaaaaatgt actgttgatt tcttttggtt aattttgttt
     7021 ctaaactatt gttttctttt acgattgctc gtaattgttc tggttccatt atctaatagt
     7081 cctccttagc cgttcctttt ctcaacggtc gtcacaaaag acattctacc atattctaca
     7141 gctagtttga tagccctttt acacattaaa gcggaataac attacgtcgc cgtcttgtac
     7201 gacataatct tttccttcta atcgaacacg tccagcttct tttgctgcat gcatgtttcc
     7261 gtaatgatcc aagtcttcaa aagagacagt ttctgcacgg ataaatccgc gttcgaaatc
     7321 tgaatggatg attcctgctg cttgtggtgc tttgattcct tttttgaatg tccaagcacg
     7381 tacttcttgt tccccagcag taaagtaagt agctaagcct aacaaatcat aagcagcacg
     7441 gatcaactga tccaaacctg attcttcgat tcctaaagct tctaaaaatt ccgctttgtc
     7501 ttcttcatct aattctgcga tttcttcttc tgcacgagca ctgaccacga tcacttctgc
     7561 attttcttct gcagcaaagt tgcgcacttc ttgtacataa tgattcgcgt ctgcatctgc
     7621 tacgtcatct tctgagacat tggctacata aagaattggt tttgtcgtca ataaaaatag
     7681 tgatttaacg attttttgtt cttcttcagt aaattcgatc gttcgggcag attttccttc
     7741 ttccagtaca ggtttgattt tatccagtac agctagttct gccactgcat ctttatcttt
     7801 ggttttagca acttttgcta cacgagtata gcgtttgttg atagattcta agtctgctaa
     7861 cactagttcc aagttgatcg tatcaatatc ttctagtgga tcaacacgtc cttctacgtg
     7921 agtaatatta tcatcatcaa aacagcgaac aacgtgacaa atagcgtcta cttggcggat
     7981 atggcttaag aattgatttc ccaaaccttc tcctttactt gcgcctttta cgattccagc
     8041 aatatctgta aattcaaaag tcgtcggaac agtttttttc ggatgtacaa gttctgttaa
     8101 gcgttgcaaa cgccagtcag gtacttctac catccctacg ttggggtcga tcgtcgcaaa
     8161 gggatagttt gctgcttctg ctcctgcttt tgtgatcgca ttaaacaaag ttgattttcc
     8221 tacgttgggc aacccaacaa ttccagcagt caatgccata aatttattca ctctttctct
     8281 gcattttttt caattacttt ttttaatttc ttttcaaaat ctcgacgggt catcatgacg
     8341 atatgtccgc aattcataca tctgattttg atatctgcgc ccattcgaat gacttcccag
     8401 cgattcgtct ggcaagcgtg aggttttttc atttcaacga tatcgtgcaa gtcgtacata
     8461 aaaatagcct cctttttgtt tattcttcat caaattgaat attcaagata tcaagaattc
     8521 gagtcaaatc tttttgagaa agatattcaa tctcgatctt acctttccca tttttttctt
     8581 ggatagctac acttgtacca aatttatcca tcaaacgatc ttcgctttct cggatataat
     8641 atggtttttc ctttgctagc cttgggattt ttttcttttc ttttccatca ttttgattta
     8701 actcagaaac aagctgttcc agttggcgga cagttaaatt ttcagttact gcacgattgg
     8761 ctaatttcaa gatcaatgtt ttatttttta aacctaacag cgtacgggct tgtcccatag
     8821 ataaacgctg atcttgtacc atttccttga caagatccgg taaagaaagc aagcgcaaat
     8881 aattggcgat ataaggtctg ctctttccta aacgactagc tacttctgct tgtgttagtt
     8941 tcaggttttt catcagcatc tcatatgctt ctgcttcttc taacggattc agatcttctc
     9001 gctgcaagtt ttccagcact gctacttgca tcatcgcttc ttcgtcaaag tctctaacga
     9061 tagcagggat cgtttctttt tcagctagtt tggacgctcg aaaacgtcgc tcccctgcaa
     9121 taatctcata tccttttact gcggaacggc gtacgatgat gggttgaaag acacctgatt
     9181 gttcaataga attagctagt tcctgcaaag atacttcttc aaatgtttta cggggctgat
     9241 acggatttgg tcgcagctca ttcaatggta gctggacaac ggtatcatct tttacatcca
     9301 ccgtctctaa gttttctaaa tcttgaaaca gtgcatcgat tcctcggcct aatcctttac
     9361 ttttcttcac gacttaccac ttcctttgcc aatgcttggt agacttcagc tcctcgagaa
     9421 cgtggatcgt agtcgatgat cggttttcca tgactaggtg cttctgataa acgaatattt
     9481 cttggaatga tcgtttcgta aactttttcg cggaagtatt tacgtacttc ttctacgact
     9541 tcatttccca gatttgttcg tgcatcatac attgtcagta agactccctc gatctctaat
     9601 tctggattaa agtgtttttg gactaaacga accgtattca gtaattggct taatccttcc
     9661 aatgcgtaat actcacactg aacaggtatc aaaatcgaat cacttgctgt aaatgaattg
     9721 attgtcagat ggccaagaga aggcgggcaa tcgatcaaaa tataatcata ctgactgctt
     9781 acttctgcta aagatccttt taatcttgat tctcttgcca tcattgacgt caattcgatc
     9841 tctgctcctg ctaattggag agtcgcaggc acaatactca aattttcgtg ttcagtgatc
     9901 aaagttgcct catcaattgg caattcattg actagaacat catagatgtc tcttgtcaca
     9961 tcaggttttc tgatcccgac accactggta gcatttcctt gtgcatccat atccactagc
    10021 aatacttttt tgccaaggct agcaagacaa gcacctagat tcaccgtcgt cgttgttttg
    10081 ccgacgccac ctttttggtt cgcaacagat attatttgtg ccatctcttc tcctcctatt
    10141 tgattggctg cttattaggc agtccgggct ttcttgggta tttctttggg gttggtttct
    10201 ttttttgaat aacgatgata tgccgttgat cagagacatt cggcaaggta accgcctctt
    10261 ctttgaggaa tttaccacct aataaagcaa tcgcttgttt tgcttctgtc aattcctctt
    10321 cactttttgc tgctttcaat gcataaaaat agccttcttg tttagccaag ggcaaacaca
    10381 attccgctaa cacattcaaa cgagcaaccg cacgagcagt gacaaggtca aatgatgcac
    10441 gaaagtgagg attttgccca aatgtttctg cccggtcatg atataaggaa gcatcgattc
    10501 ctaataatcg ggttaactca gataagaagg ttattcgttt attcagagaa tcaacgatcg
    10561 ttatctgcag ttctggaaag gcaattttta aaggtatgct aggaaaccct gcccccgaac
    10621 caacatcgca taaagataaa ggttgcgtaa agtctacttc aaatgctaat gtaagtgaat
    10681 cgtaaaaatg cttcagatat acgtcttttt catccgtaat agctgtaagg ttcatctttt
    10741 cgttccattc gactaatgtc cgataataaa tagcgaattg attcatttga tgatcggtaa
    10801 gctctatccc tttttctgcc aaggattggc gaaattcttc tggtatcata aatattcctt
    10861 tcattgtgaa acagcaattg tttcacagat tctctcccta ctttaacgca aaatctctag
    10921 acatgcaatc cttccatatg gattcttaaa aatttgaaaa gtaagaaaag ccgatagact
    10981 ttttctttct agtatgactc ataaaatata cacagaagag agaactagct ttgagcaatt
    11041 tctgcaaaaa ctagttctct cttctattta ttattacgta ttacgctggt tcactgactt
    11101 tcgcaatctt cccttgttcg atatagacca ttaggatact taggtctgca gggtttacgc
    11161 cactgattcg gctagcttgg gcaattgttt ctggttggat tttctgcaat ttttgttttg
    11221 cttctgttgc taatccgttg attgctgcgt agtcgatgtt ttctggaatc tttttcgctt
    11281 ccatgcgttt taatttttct actttttcca tggctttttt gatgtagcct tcatatttga
    11341 tttggatttc tacttgttcg atttcttttc gtgtcaattc ttcattttct ggaataaacc
    11401 gtagaagttc tgcataagta atctcaggac gtcttaaaaa gtcgcttgct agtacaccat
    11461 cttttaatgg tgccgcatta atcgttgcta aaaagtcttg gacttcttct gttggtttga
    11521 ttcgtacatt catcaaacgt ttgatttctt gttcaactgc ttcttttttc ttcaaataat
    11581 tcttgtattg atcttctttt actaagccta cacggtgacc catttctgtt aatcgcaaat
    11641 ctgcattatc atgacgtaaa agcagacggt attcagcacg tgaagtcagc aaacgataag
    11701 gttcgttcgt tccttttgtt accaaatcat cgatcatgac accgatgtaa ccgtcacttc
    11761 gtttgatcac taaaggttct tttccctgaa ctttcaaagc tgcattgata ccggcaatca
    11821 gcccttgtcc agccgcttct tcataaccgc ttgtaccatt tgtttgtcca gctgtataaa
    11881 gattttcaac gactttagtt tctaatgtcg gacggagctg gtgtggctct actacgtcat
    11941 attcgatagc atatcctgta cgcatcattt ctactttttc taatccagca atagaatgaa
    12001 gcatgttgac ttgaacatcc tctggtaaag aagtagataa accttgaaca tagacttctt
    12061 ccgtatttaa gccttctggt tccaaaaata attgatgacg tggtttgtct gcaaaacgca
    12121 cgatcttatc ttcgatggat ggacaatagc gagcgcctac cccttcaacg atccctgtaa
    12181 acattggggc acgatgtaag ttgtctcgaa taatttggtg agtcgtctca cctgtatagg
    12241 tcagccagca agattcctga tctagtttat aggcactatc tggtgtgctg aagctaaaat
    12301 gattcggctc tttgtcacca ggctgctctt ctgtaacaga atagtcgatc gtacttgatt
    12361 ttactcgagg cggtgttcct gttttgaacc gttcgatagc aaagccaagt tcttttagat
    12421 gattcgctag tccaatagaa ggttgagagt tattcggacc tgatgaatat ttcaattcac
    12481 cgataatgat ttctccgcga agtgctgttc cggctgtaat aacaactgct ttggcccggt
    12541 agctcgctcc tgtggacgtc actactccat gacaaacacc atcttcgacg atcagttctt
    12601 cgacgattcc ttgtcttagc gtcaaatttg gttctttttc gatcgtatgt ttcatttctg
    12661 ttgaataagc atgtttgtct gcttgtgctc gcaatgctcg gacagctggg ccttttcctg
    12721 tgttcaacat acgcatttgg atataggttt tatcgatatt cttacccatt tctccgccta
    12781 acgcatcgat ttctcgaacg acaacgcctt tagctggtcc gcctacagaa ggattacatg
    12841 gcatgaatgc taccatgtcc aaatttattg tcagtaataa tgtttttgat cccatacgtg
    12901 cagcagcaag tgcagcttct gaaccggcgt gtcctgcgcc aacaacgatc acgtcatacg
    12961 catctgcttc ataatgttcc aatcgtttgt tcctcctttt attttcctaa acaaaattga
    13021 ctgaataatt gagtgattag ctcatcttgt acactgtcgc cgacaacttc tcctagatag
    13081 tcccagcaac gcgtcatatc tatttgaacg agatccacag gcattcctga atcaatacct
    13141 gcaatgactt cttcaagtga gagggaagct ttttctaaca acgcgatatg gcgggtatta
    13201 gaaacataag tagcgtctct ttctcctgtt tctccgctga agaataaatc agaaatagct
    13261 gattccaaac gatctagtcc gtcgttttta gctacggaaa ttgaaaaaac tggttcattt
    13321 tcgatcaatt tcttcaattt ttcttgttcc agctgtgctg gcaaatctgt tttatttaga
    13381 agaatgatcc gtttcaatcc acttgtcgct tccaataatt gttcatcttc tgctgtcagt
    13441 ggttcgcttt ggtttagaac aagtaagatc aagtctgctt ctgctaatgc ttttcggctt
    13501 ctttcgaccc cgatccgttc caccacatct tctgtttccc gaattccagc tgtatcgatt
    13561 agcttgagcg gtaccccccg aacattgaca tactcttcga ttacatctcg agtcgttcct
    13621 gcaatatccg taacgattgc tttttcttct cttaacaaat ggtttaacaa acttgatttg
    13681 ccgacatttg gccggccgat gattgccgta cttaaccctt cccgcagaac cttcccttgc
    13741 ttggatgtag caagtagcgc ttggattctt tgttggatca tctgcgcttt ttcaagcaat
    13801 aacttcgtag tcaattcttc gacatcatca tattctggat aatcaatatt tacttctact
    13861 tgtgctaatg tctctaaaat ctcttgtctc aatgagcgga tcaaagcaga cagattgccg
    13921 tctaactgat taagagctag tcccattgct ttgtctgttt ttgcccgaat caaatccatc
    13981 acagcttctg cctgtgaaag gtcaacacga ccattcaaga atgctcgttt tgtgaattcg
    14041 cctggctcag ctaatcttgc accttctctt aacaatagct gcaaaatttg attgactaca
    14101 acgatcccac cgtgacaatt gatttctact acatcttccc gcgtaaatgt ctttggtgct
    14161 ctcatcacgg agaccatgac ttcatcgaca agctgattgc tcttgggatc aacgatatgt
    14221 ccataatgga tcgtatgact tggtacttct gataatcgtt tgtttccaga ttgataaact
    14281 ttatctgcta gttgcacagc ttgatcgcca cttaaacgaa cgatactgat tgcaccttct
    14341 ccaggtgggg tagaaattgc cgcaatcgta tcaaattcta aagtaatttc agccattttt
    14401 tcacttcctt tttcattttg cgcacaaaaa aagtgcccac tccacccttt atgcacaata
    14461 aacggtgaac ttcgcacttt gactgcttgt tcttgattaa attctggtca tagattatca
    14521 cacttgctat aaggaatcaa gtgtttaaaa caattagatg aagttgagcc tgttctgctt
    14581 ccattacctt atatttaatt gtccttggat gtctctttgc gttctttggt ttattcgaag
    14641 agaagatact gtataaacaa ggctttatct ttcttaaaaa aaacggtaca ctaaatggta
    14701 gcgctatcta aactatacac ttttgaaagg agaaaatatg tgagcccgtt cagatctgct
    14761 gcattggaag aagaaagtga ccctcggatc aacgatgtcg ttttatctac agggaatatc
    14821 aaccagaaat atatcgtccg cgatatcgtt tttgttgtgg aatgcttaga agctgatttg
    14881 tttgacccat cttttcccaa agatgacctt ttcttaacaa cgaagaggaa attaaagacg
    14941 aaggtacttg aatatggtgc gaatgcagta atcaactgtc attttgagta tcagcaaaaa
    15001 aaacgcgacg gaaagtattt tctggaactt tttgcttatg gaacagtgat ccaatttatc
    15061 caaacaacga ttggttaaat gaaacagaca aaaaaagcgg atcaaaactg aagacagttt
    15121 cgatccgctt ttttctatca aagttagtga ctacttcatc gtatactctg ataagaatga
    15181 tttctagtaa aatatacttg gtttgaaaaa ctttgtcaac gtgatttatt caaaaagtaa
    15241 tggaatcaat aatatttttt agcaggttct actactaaat aacgataagg ttcctcacct
    15301 tccgaatgtg tttttacatg ttcatctttg cttaatgcag catggatttg tttacgttca
    15361 aatgcaggca ttggttctaa gaaaacaggc tgccctgtcc gttttacttt ttcagccgtg
    15421 cgatcagcta aacgctgaat gatctcttgg cgcttttcac ggtaattgcc tacattgact
    15481 actacagaca gcttatttct tgctaaacga tggatgaaga cttgtgccag atattgcaat
    15541 gcattcagtg tttttccatg tttcccaatc agtatgcctt gcttttgtgt ttctaaatgg
    15601 aaaacaacca tattatttga ttctctttcc attttgacaa gagctggagc atcaagctgt
    15661 tttgaaattt cagtaagata aactgccaat tccgttaaag ctgcttcatc acctagctca
    15721 cttacttctt ctgttttttc ttcaagaatc gtttcgttga ctacctcgat ctcagctgtt
    15781 ggttcttctt ccaaaatttc ttctacagtc tgttctactg tttcagaaac tttttcgctg
    15841 atacttggct ggatcgaaag ccgtgcattc tttcgaccca ttcctaggaa accttttttt
    15901 ccttcttcca gtatttcgat ttcgacttgg tctttggcca aacccaaatc agccagacct
    15961 agttggacag cttcgtctat tgttgcagct tcataaatcg gcattagcat tacctcccct
    16021 attatttccg ttttttctta ggattcattg ctttcttaag agcacgttct ctttcgcgga
    16081 ttctccgtgc ttcttcttca cgctcttttc gaattttgaa agggttgttg atcagcagcg
    16141 tttgtacaac ttggaaggca ttggaaacta cccagtataa tgaaagaccg ctggctaaat
    16201 tgacacccat cactaaaatc attacaggca tcacgaaatt catgatcttc attgatgcat
    16261 tcgattcaat ttgactcatg ctagataaat acgtacttgc aaaagtaaag attgctgcta
    16321 aaatcggcaa aataaatgtt ggatccgcgt tccctaagtt caaccataag aaatgccctt
    16381 gtttcaactc tggtacacgt gaaatagatt gccataacgc catcaagatt ggcatttgta
    16441 ctaataaagg aagacaacca gcatatggat tgacgccatt ttctgcatat aaacgctgct
    16501 gctcttctct taacttactt tgtgtttccg gatctcttga actatattgt tgctgcagtg
    16561 cttttaattt cggttgtaat tcttgcgttt tacgcatgct ctttgtttgg aaatgcatca
    16621 acggcaatag gatcacgcgg atgatcagtg taaataaaat gatccctatc cctgcattac
    16681 caaatgacaa cgccttgatc gcctcagcaa agtaataaac aatataacga tcccaaattc
    16741 ctgtactctg tgcgctgact tccccagttc cgcaaccgga taagacgatg accaaactaa
    16801 cgataccggc catcaaaagt aaacgtttgt atttcttcac ttactctttt cctctctgtc
    16861 gattattttt gcaagtttta agacgtggac taaattggag cgaacctcat cataagtcaa
    16921 ccccttcaca cttggtctag caatcacaat aaaatccaac tccggatcaa tttcttcttt
    16981 tatagaataa actgctgctc ggatttttcg tttgacttca ttgcggttta ccgcgttgcc
    17041 gacctttttt cctacagata aaccaactcg aaaatgaggc tgtcccggtt tttccaaacg
    17101 gtaaacgacg aatttgcggt tagcaaaaga atctcccgta tggaacacgg cttgaaactc
    17161 tcgttctttt tttactcgat acgcttttct cattagtttt ctttcctttt ctatctctca
    17221 cttacgctca taatcatacc ataattacga ttgtcctttc atcatttccc taaaaaagag
    17281 tataaaaaaa accactgaaa ccccagtggc ctaagctgaa agaacttttc ttcctttgcg
    17341 acgacggcta gctaacacac gacgtccatt tttagtactc atacgtttac ggaatccgtg
    17401 aactttttga cgtttacgtt tatttggttg gtatgttctt ttcatatatt ccacctccaa
    17461 aatgttctta tctattttat aaacacagac atactttgat agtataacga caaagtagtg
    17521 attttgtcaa tataaaatag aagatcgatt gttaaaactt atccacaaga ttattttttt
    17581 ctatgggagt cctatgtgga aaaagttatg cacagggatt atccacagat aataaatggc
    17641 tggttttaca cagaaaacac acgtgtaaaa aaagtaatcc acaatcaggt gatttcctgt
    17701 gtattccgtc tcctaactat tggtatatct atatttttta tccacagatg caagaggaaa
    17761 tctattgatt tttacgagtt ttccacctgt gcattagtgg tgtggaaaac ttttttttga
    17821 caactgtgtt tttctctttt ttctttttgt cgattgtgtg gataattgta attaaaaaat
    17881 ttttttcttc ggtttctgtg gaaaactatt gaaaaaaatg ctaaattagt actagttatt
    17941 cactttttaa tgaaggagga ggccttatat ggtatccctc gatgctttat ggaatgaatt
    18001 aaaagcaaca taccaaaagg atttatcgcc tgctagctat aatacatgga tcgaaacagc
    18061 gaaccctcga acccttgatc aaaaccagct cgttgttgag gttcctagca agattcataa
    18121 agaatattgg gaaaaaaacc tggcgacaaa aatcgttgaa gtcggttata tgttatcagg
    18181 aaatgagatt atccctcgct ttattactgg cgaagaagca gagcaagaag aagttataga
    18241 agaaaaaaat ccaaaagttg tggcacctag tccgctaaaa aaagccatgc tgaaccctaa
    18301 atataccttt gatacgtttg tcattggtaa aggaaaccag atggctcatg cagcagcgct
    18361 cgttgtagca gaagatccag gttctattta taacccgctc ttcttttatg gaggcgtggg
    18421 acttggtaag actcacttga tgcatgcaat tggtcatcaa atgctgcaaa gtcagcctaa
    18481 tgccaaagta aaatatgtaa gcagtgaaac ttttgctaat gatttcatca attcgatcca
    18541 aaacaaaaca gcagaagagt tccgtcaaga atatcgaaat gttgatttac tgctagtgga
    18601 tgacattcaa ttttttgcag aaaaagaagc tacgcaagaa gagtttttcc atactttcaa
    18661 tgccttgtac aatgaaggga aacaaatcgt actaaccagt gatcggttgc caaacgagat
    18721 tccgaaactg caagaacgcc tcgtttctcg ctttgcatgg gggctgtctg ttgatattac
    18781 accgccggat cttgaaacaa gaacagcgat tttacgtaaa aaagcggcgg ctgaacgtct
    18841 agagatccca gatgacacac taagctacat cgcaggacaa attgattcta acattcgtga
    18901 attagaaggt gcgcttgttc gagtgcaagc ttttgctgcg atgaatagtg aagatatttc
    18961 gactagtcta gctgcagacg cactgaaaac gttaaaatca ggaaaaggac atcctcaatt
    19021 gtcaatcctg cagatccaag aagaagtggc aaagtattat cacatccaat tgaaagatat
    19081 caaaggaaag aaacgcgtca aatctattgt tgttcctaga caaatcgcta tgtatttggc
    19141 acgtgagctc acagataatt ctttaccgaa aatcggagct gaattcggtg gcaaggatca
    19201 tacaacggtt atccatgctc atgaaaaaat ccagcagctg atggattcta gtgtcagtat
    19261 gcaaaatgaa gtatcagaaa tcaaaaactt tttactaaat taatttgtcg attctatcaa
    19321 ctgaaaatag catgtggata aaagaaaaaa acgttcaaaa gttatgcaca ctttttccac
    19381 agctgttttt tcgtttctct cttacgtttt ttgagttttc cacagaatca acatgcccta
    19441 ttacttttat tattcttttt aactaataat aaataaacaa acattcaaag gagatacatc
    19501 atgaaagtta ctttaaaccg agctagcttt atgcaggaat tgcaaactgt tcaacgagct
    19561 atttcaagca aaaccacgat ccctattttg acaggtgtaa aaatcacact gacacaagaa
    19621 ggtttgactt tgacggggag caacgctgat atatcaattg aaactttttt gtctgttgaa
    19681 aacgaaaaag caaatatgca aatcgaatct actggttcca ttgttttaca agcacgtttc
    19741 tttagcgaaa tcattcggag acttcctgaa gaaacattta ctttagaagt tttagaaaat
    19801 aaacaagtag cgattacttc tggaaaagcg aattttatcg taaatggatt agatgcagat
    19861 aactatcctc atcttcctgt tgtcgaaagc cataaccaga tgaaattacc tgtacacgta
    19921 ttgactaaac taatcaacga aacagttttt gctgtctcac aacatgagag tcgtccaatc
    19981 ttgacaggtg tgcatttcat tttatctgat aattctttat tggctgtagc tactgattct
    20041 caccgtctaa gtcaacgcgt gattccagta gaacaagcgg ctgatcattt tgatattgtt
    20101 attcctggaa aaagtttgat cgaattatct cgctcattaa caaatgaaga agaaatcgtc
    20161 gaaatcagca ttatggaaaa ccaagtgttg ttcaaaacag aaacgatgta tttctattcc
    20221 cgtttgctag aaggaaacta tcctgatacc aatcgtttga ttccttcaag ttttaataca
    20281 gaggttgaat tttctgttcc tagcttttta gcggcgatcg aacgggcttc tttactttct
    20341 catgaaggcc gtaacaacat cgttcgttta tctattcgtc cagatgcggt tgttttatac
    20401 ggaaattcgc cagaaatcgg gaaagtagaa gaaagcttga gttatacagc aagcagtggc
    20461 gatccattag atatttcttt caatccggat tatatgaaag cagcgttgcg tgcatttgga
    20521 gatatgagca tcaaagtgaa atttatctct gctattcgtc catttacatt ggaaccaacg
    20581 gaagatggcg ttcaatttat tcagctgatt actcctgtac gtacaaatta acaatgattt
    20641 tcattgttct tgaaaattta attgaaaaga aatagaaaaa ataaaatttt aaacagaagg
    20701 ccctaaaatc tcttttaagg ctttctgttt atttttgttt aaatcacctt ttttagatta
    20761 aatcgtctta tatggaaaaa tatatggata aatttgtttt attgcgaaaa aaaaggtata
    20821 atagagtata cttttaataa tgaaaatgag ggatgggcat tgaaacaaca agtcattcta
    20881 aatacggaat tcatgacgtt gggccaaatg ttgaaagaag tcacagttat cggtagtggc
    20941 ggacaagcaa aatggtattt ggcagaaaac actgtgttag tcgatggcga accagaaaat
    21001 cgtcgtggac gcaaacttta tcccggcatg atggttgaag ttccagaagt aggaactttt
    21061 tttatggtaa aaaaaggaaa cgacgataat gaggctgaat aagctgtatt taaaaaatta
    21121 tcgaaattac gaagagctga acatcgaatt ttccaagaac ctcgtcatct ttttaggaga
    21181 aaatgcgcaa ggaaaaacga atcttttgga aagtatctac gtattagcta tgacgcgaag
    21241 tcatcggaca agtagtgagc aggaattgat tggctggact gacgatcagg cgatgattca
    21301 aggagagatc acaaaaggtt catccactct tccattagaa atcttattat ctaaaaaagg
    21361 ccgaaaaacc aagatcaacc atatcgaaca aaaaaagtta agtacttacg tgggacaaat
    21421 gaatgtcatt ttatttgcac cagaagatct ttctttagta aaaggcagcc cgcagatcag
    21481 gcggaagttt ttagatatgg aattaggcca gatcaatcca atctatcttt atgatcttgt
    21541 ccaataccaa gcaattttga aacaaagaaa ccagtattta aagcaattga atgaaaaaaa
    21601 acaaactgat ttgctttatt tggatatttt gtccgaacaa ctagcagcgt ttggcagcaa
    21661 agtattgaaa gcaagagcac agttcgtcaa gcgattggag ttttgggcta attctttgca
    21721 tcagcaaatc acccatcaaa aagagcagct ggaaatcgag tatctcaccg cagtcgattc
    21781 tttagaaaca catacgcaag agcaaatcca agaacaattt ctcgcacttt tgaaccaaaa
    21841 taaaaagaaa gacctttttc ggggaacaac gacagttggt cctcatcgtg acgatctttc
    21901 cttttttatt aatcagaaaa atgtgcagac ttatggatcg caaggacagc aaagaacaac
    21961 tgcactaagt gtcaaattag cagaaatcga tttgattaaa gaagagacgg gggaatatcc
    22021 aatccttttg ttagatgatg tgatgagcga gctggatgat agtcgtcaac tccatctgct
    22081 agaaacaatc gaaggaaaag ttcaaacgtt cttaacaaca acgacacttg accatgtaaa
    22141 aaataaaatg actgttgaac ctgagatttt ttatgttcaa caagggaaac taaaaggaga
    22201 agaaactgaa tgacagaaga aagaagttta gtagaacgcg ctaaagagta tgatgccagt
    22261 cagattcaag tacttgaagg acttgaagcc gttcgtaaac gacctggcat gtatatcggt
    22321 tcgactagtt ctgagggtct gcatcacctt gtttgggaaa ttgtcgataa ttcgatcgat
    22381 gaagtattag caggttttgc aacgaaaatc catgtgatta ttgaaaaaga caacagtatc
    22441 accgttattg acgacggtcg ggggatacct gtagatatcc aggcaaaaac tggacgtcct
    22501 gcagttgaaa cggtctttac tgttttacat gctggaggaa aattcggcgg tggtgggtac
    22561 aaagtttctg gtggacttca cggagtagga tcttctgttg ttaacgcgct ttctacacag
    22621 cttgatgtca aagtatacaa agaaggaaat gtgtactatc aagaatatcg tcgtggagct
    22681 gtagtggacg acttaaagat tatcgaacaa acggatcgcc atggaacaac ggttcacttt
    22741 acgccagacc ctgaaatttt tacagagaca actgagtttg atttttcaaa attggctacc
    22801 cggatccgtg aacttgcttt cttgaaccgc gggatgcgta tatcgattga agacaaacga
    22861 gaagaagaac cagtcatcaa tgaataccat tatgacggcg ggattaaaag ttatgtcgaa
    22921 tacttgaatg caaacaaaac cgtgattttt ccagaacctg tatatttgga aggtgaacag
    22981 caagatatcg cagttgaagt ctctatgcaa tatacagacg gctaccattc caatatcatg
    23041 agttttgcca acaatatcca tacgtatgaa ggcggaacac atgaatcagg attcaagact
    23101 gctttaactc gtgtgataaa tgattatgcc cgaaaacaaa agctgatgaa agaaaatgac
    23161 gacaacttga ccggtgaaga cgtgcgagaa ggattgacag ctgttatttc tatcaagcat
    23221 cctgaccctc aattcgaagg acaaacaaaa acaaaattgg gcaattccga agtacggaca
    23281 gtcactgatc ggttatttag cgaacatttc atgaaattct tgctggaaaa cccaagtgtg
    23341 ggccgtcaaa ttgttgaaaa aggattacta gcttccagag ctcgtctagc agcgaaacgt
    23401 gcgcgtgaag taacgagacg taaaggtgct ttagagatta gtaatctgcc aggaaaatta
    23461 gcggactgct ctagcaacga tcctgaaaaa tgcgaattgt tcatcgtcga aggagattcg
    23521 gccggcggat cagctaagca aggtcgtaat cgagagtttc aagccatctt gcctattcgt
    23581 gggaaaatct tgaatgttga aaaagcaagc atggacaaga tcttggcaaa tgaagagatc
    23641 cggtctttat ttactgctat gggtacaggg tttggagaag attttgatgt atcgaaagct
    23701 cgctaccaca aattagttat catgactgat gccgatgtcg acggcgcgca tatccgtaca
    23761 ttgctgctga ctctttttta tcgttacatg cgtcctattg ttgaagctgg atacgtctat
    23821 attgctcagc cacctttata cggggtaaaa caagggaaga atattacgta tgtacaacca
    23881 ggaaaaaatg cagaagaaga gttgaaacaa gtcgttgctt cacttcctgc ctcaccaaaa
    23941 ccaagcgttc agcgttataa aggacttgga gaaatggatg atcatcaact atgggaaaca
    24001 actatggacc caagcaatcg gatgatggcc cgcgtcagtg tggacgatgc tattgcagca
    24061 gatcagatat ttgaaatgtt gatgggggat cgagtagaac cccgtcgtgc attcatcgag
    24121 gaaaatgcac actatgtgaa gaacttagat atttaatcat gaaggaggaa aaagaatgag
    24181 tgaagaaatc agagaaaaca tccatgatgt caatctaacc agcgaaatga aagactcctt
    24241 catcgattat gcgatgagtg ttattgtcgc acgtgcactt cctgatgtca gagatggtct
    24301 gaaacccgtt catcgccgta ttttatacgg catgaacgaa ttaggtgtaa caccggataa
    24361 agcccataaa aaatctgcca gaatcgtcgg agatgtcatg ggtaaatacc atcctcatgg
    24421 agactatgcg atctatgagt caatggtacg gatggcacag ccattcagct accgttatat
    24481 gctagtggat ggtcatggaa actttggttc tgttgacggt gacggtgccg ccgctatgcg
    24541 ttataccgaa gcacggatga gcaaaatcgc tacagaaatg ctgcgtgata tcaataagaa
    24601 cactgttgat ttccaaagca actatgatga tacagaaaaa gaaccagttg tactacctgc
    24661 tcgcttcccg aatcttttag tgaacggaac aacggggatc gccgtaggta tggccacgaa
    24721 tatcccacct cataacttgt ctgaggtagt agcggctatc gatttgctga tggataatcc
    24781 tgatgtgacg accaatgaat tgatggaagt attacctgga ccagattttc caacaggtgg
    24841 tttggttatg ggaaaatcag gaatccgaag agcgtatgaa acaggtaagg gttctattac
    24901 cgtacgtgca aaagttgaga ttactgagat gccaaatggt aaagaacgga tcatcgtgac
    24961 agaattgcct tatatggtca ataaagcaaa attgatcgaa cggatttctg aactgcacag
    25021 agacaagcgt atcgaaggga tcacagattt gcgggacgaa tcttcccgtg aaggtatgcg
    25081 gatcgtcatt gacatccgtc gtgacgccag cgcatctgtt atcttgaata acttatataa
    25141 attgacatcc ttgcaaaatt catttggatt caatatgttg gcgatcgaaa aaggtgtgcc
    25201 gaaagttttg agcttgaaac aaattcttga aaactatatc gaacatcagc gcgaagtcat
    25261 cacaagacgg acagtctttg aaaaagaaaa agcagaagca agggcacaca ttttagaagg
    25321 tttgcgtatt gctttggatc atatcgatga aatcattgcg attatccgcg ggtctaaatc
    25381 agatgacgaa gcaaaagcga cgatgatcga acggttcgat ttatccgatc gccagtctca
    25441 agctatttta gatatgcgtt tacgccgatt gaccggtttg gaacgggaaa aaatcgaaaa
    25501 tgaataccaa gaactcttaa aactgattga agatttaact gacattctgg caagaccaga
    25561 acgtgttaca gaaatcatca agaatgaatt agcagacttg aatcaacgat ttggtgataa
    25621 acgacgcaca gaattactag tcggtgaggt cttaagtctg gaagatgaag atttaatcga
    25681 agaagaagaa atcgttatta cattgacaaa caatggttac atcaaacgtt tggcaaataa
    25741 tgaattcaga gctcaacgaa ggggtggacg tggtgtccaa ggaatgggta tccacaatga
    25801 cgacttcgtc aaaaatctgg tttcttgttc tacccatgat acattgttat tctttacgaa
    25861 taacggaaaa gtgtatcggg caaaaggata cgaaattcca gaatacgggc gcacagccaa
    25921 aggaatacca attatcaatt tactaggtat cgattcgagt gaaagcatcc aggcaatcat
    25981 tgcggtgacg ggagaagcag aagaaggcca ctatctcttc tttacgacgc gtcaaggtac
    26041 agtaaaacgt acttctgtca ctgcttttgc taacatccga agtaatggtc tgatcgcaat
    26101 tggtttgaaa gaagacgatg agctcgtcaa tgtgctactg acagatggtc aatctaatat
    26161 cattattgga acacataacg gctactctgt cactttcaaa gaagaagcag tccgcgatat
    26221 gggacgtaca gcttccgggg ttagaggaat acgcctgcgt gaggaagatt atgtcgttgg
    26281 ggcagcctta atgaaggaag atcaagaagt attagtgatc acagaaaaag gttacggaaa
    26341 acggacaaaa gtatcggaat atccagtgaa aggccgcgga ggtaaaggga tcaaaacggc
    26401 gaacatcact gaaaaaaatg gtccactagc tggattgacc actgtttctg gagatgaaga
    26461 catcatgctg attacagata aaggtgtcat tatccgtttc aacgtatcga ctgtttctca
    26521 aactggacgt tcaacattag gcgtacgttt gatgaagatg gaagctgata cgaaagttgt
    26581 cacgatggca gcagtcgaac cagaagctgc agaacaaaca gttgaagaac aaggatcaga
    26641 agaatagcca tcaagactaa ttgtgtaagc tagcaagaat gaccgattaa aaaatctggt
    26701 tattcctgct ggcttttttg attagttgaa tttctcttaa aatattcgaa actgttcatt
    26761 ctgtattgct tttgaccaaa atagagtgta taatcagagc ttgtgagtag tctaattaat
    26821 tagataactc tccttgctct taggaaccta agagccgaaa gtccacaggg aggtgaatat
    26881 caatggaaaa cacgaaatat gaaatcatgt acattattcg tccaaacatt gatgaagaag
    26941 caaaaactgc tttaatcgaa cgtttcgatg caatcttgaa agataacgga gcagaagtta
    27001 tcgaatcaaa agattgggaa aaacgccgtt tcgcttatga aatgaacggt taccgtgaag
    27061 gtatctatca catcgttaaa gtatcttctc catcatcagc aaatgctgtc aatgaatttg
    27121 accgtcttgc taaaatcaac gacgatatca ttcgtcacat gattgttaaa gaagaagctt
    27181 aattgttgtt tcatgtgaaa caatagaaaa cgatgagagg agatgactat tttgattaat
    27241 aatgttgtat tagttggacg tctgacgaaa gatccagatt tgcgttacac agcaagtggt
    27301 acggcagtcg caacatttac tttggctgtg aatcgtaatt ttacaaacca aagcggcaat
    27361 cgcgaggctg attttatcaa ctgtgtgatt tggcgcaaat cagcagaaac tttagcaaac
    27421 tatgctcgta aaggaacact tttaggtgtt actggacgta tccaaactcg ttcttatgat
    27481 aatcagcaag gacaacgtgt atacgtgaca gaagtagtag ctgacaactt ccaattgctg
    27541 gaaagtcgtt ctgcatctga aaatcgccaa caaagcggcg gattccaaag ttctggtcaa
    27601 tcagctggcg gatttggcgg aaacaataac tctaatcaaa catctcaatc atccaacggt
    27661 atgcctgatt tcgaccgtga tacttctgac ccattcggct caagttcaac gatcgacatt
    27721 tcagacgatg atttaccatt ctaaacgaat ttgataggag ggaaatagaa tggcacaaca
    27781 aagaagaggc ggacgtaaac gtcgtaaagt agactacatt gctgctaacc atattgaata
    27841 tatcgattat aaagatatcg aattactaaa aagatttatt tcagaacgcg gtaaaatctt
    27901 gccacgtcgt gtaactggta caggcgctaa aaaccaacgt aaaattactg ttgcaatcaa
    27961 acgcgctcgt atcatgggct tgctaccttt cgttagcgaa gactaataaa tcctaggaat
    28021 tgaatgactg atccttttga tcagctattc aattcctttt tttctgtaaa aataacctct
    28081 attcgtttag tcgctcgaat gacgaaagtt atggtaaaat caaattagtc atatatggga
    28141 ggaaaattat ggaaaataaa aacaatcatt tatccccgaa acctctcttc cttatcctgt
    28201 ttatattaat cgaagtattg gtagtcttaa ttttaccatt tcgttttgtt agcgcttcgg
    28261 tcattctatt gatgaattta tggggtgttc gctattggtt gaaaatgaaa cgttggctta
    28321 ttttaaataa acgtgaaaaa atccagcagg cttcgactgt agctgaaacc aatctagctt
    28381 atgtctcaga agtcatgcct gtaggagtga ttgcttatca gccatcaagc caaaaaatcg
    28441 aatggctcaa tccattagca ttgaaaatca aagatcaaag tggattaaca gaggaagaat
    28501 tgctagatac tttttttact gcaaaagaaa aaaagaaaaa ccaagtctat ctagcagata
    28561 aaacatttca gtttgatatg gatacagtaa agggtgttgt ttattttaat gatattaccc
    28621 aaaaccagtt gttaaaactc catcagacga acagtcagcc ggtgattggt attttatcta
    28681 ttgataacta tgcagatgcc attgataaat tggatgacaa agagatttct tatttgaact
    28741 ctttcgtcac tacactagtt tctgactgga tgaatgaata ccatgtcttt ttcaaaagaa
    28801 tcaacgcaga acggtttttc ttcgtagcac agatgatcga tctgaaaaag atgatggata
    28861 aaaaatttga tttgctggat cgctttcgag cagaagctga aaaacagagc tcgcctatca
    28921 cccttagtat ggggattgct tatggctcaa gaaccctcga tgaaatcggt ggagaagctc
    28981 agaataattt agatgttgct ttagtccgtg gaggcgacca agttgtcgtg aaagaggtaa
    29041 aagaaggagc aaaacctgtt tattatgggg ggaaatctgc taagaatgtc aaacgcacgc
    29101 gtgttcgttc tcgagcgatg agtaccgctt tgcgtcggat ttttgatgaa acaggcgatg
    29161 tgtttatcat ggggcacaag tttccggata tggatgcttt aggctctgct tttggtgtca
    29221 gttgtctggc acgcttcaac cagaaaaaag cttacatcgt tatcaacgag aacgagatca
    29281 ttccagatgt agaacggtgt cttgaagaaa tccataagca tcctgaatta gaaatgcaac
    29341 taatctctcc agaacgtgcc atagagctga aaaaagaaga ggacctcctc gtgatggtgg
    29401 attaccataa accttctcta tcgatctctc agggcctata tgagcaattt gataagattg
    29461 tgatcattga tcaccatcgt cgtggggaag agtttccaag taaacctctg ctgacttata
    29521 tcgaatcttc agcttcttca gcttcagagc tagtttctga gctgatccaa taccaaagca
    29581 gcaaaacaaa tcgcttagat cgttttgagg ccacattatt gcttgcaggg atggttgtag
    29641 atacaaatag tttcaatgtc cgtacaactg cacgaacttt tgacgttgcc agctatttaa
    29701 ggtcttgtgg agcagacgca tccttagtac aatatttatt gagctcggat ttagcgacat
    29761 acttggagat cagcaatttg attgctggaa gcgactatgt gacttctgat attgttgtgg
    29821 cggcagggac agacgaaaaa gagtataata gtgttatagc agcaaaaaca gcagatacat
    29881 tgctttcgat gatcaatatc aatgcagctt tcgtcattac aaagcggaca gacggcttga
    29941 ttgggatcag tgccagaagt accggcacga tcaacgtcca aatcatcatg gaatccttag
    30001 gtggcggagg acacttcaca aacgcagcta ctcaactgga aaatacgaca gttgaggaaa
    30061 caaaacagaa gctatttgct gcaatcaaag aaaatatgaa tcaaatatat gataaggagt
    30121 aatccaaatg aaagtaattt ttttacaaga tgtaaaagga aaaggtaaaa aaggggacat
    30181 caaagaagtt ccagcaggtt atgcacaaaa ttatctaatc aaaaataact tggcaaaaga
    30241 agcaacgaaa agcagtatca gtcagttagc aggacaaaag aaagcacaag aaaaacacga
    30301 agctgaaatc ttggaagaag ctaaaaagtt aaaagaattc ttggaaaaag aagatactgt
    30361 agtcgaattg aaggcgaaag caggggaaga cagccgtttg tttggttcga tcccttcaaa
    30421 acaaattgct gaagggttaa acaaacaata caaaatcaaa ttagataaac gaaaaatcga
    30481 attagaaaat ccgatccgtt cactaggcta cacaaacgta cctgttaagc ttcatcatga
    30541 ggtgacggct acgatcaaag ttcatgtcgt acaagaataa caaatcttga tagcgatgtt
    30601 cagctggtaa gtataaccag aaggaaaaaa gacaaaccga aatttatctg agaaaacagc
    30661 tgcagcaatg aaagcaaaag ctgcaaatcg atttcagatt gatttcggtt tcttttttgg
    30721 gaacagcaaa atagcataaa gaaaacgagt ataataaaag agaactattt ttttattaca
    30781 ctttcggtac gaatgatgac ttttctctca ttttcttgta aaatgaagca attgaagaca
    30841 aggagcgata cgtatgaatg aagtgtggca ggatcgaatc ccaccgcaaa atattgaagc
    30901 tgaacaagcg gttttaggct ctgtttttct tgacgcagaa acgatcattg atgccatgga
    30961 gtacatagag ccaaaagact tttatcgtcg tggccatcaa attatttttg agacaatgat
    31021 cgagttaaat gatcgtaatg aagcgattga cgtggttact gtcaaagacc ggctggaaca
    31081 agccaatttg ttagaagatg ccggtggttt aagttatctc tcagacttgg cattagcagt
    31141 tccgacagca gcaaatatcg tctactatgc gaaaatcgtt gaagaaaaat cattgctgcg
    31201 tacattgatc cagacagcaa caggaatcgt tacgaaagga ttcgagcaag gagaagaagt
    31261 tcaaacaatt ttagatgatg ccgaacgcag tatcttggag gtttcggaaa aaagaaatcg
    31321 aagcggcttt ttatctattg ctgaagtact gaactccacg attgaaaata tcgatcgact
    31381 agctcaaaac aatgaagaaa tcacaggtct accaactggc tatcaaggtc tggataaaat
    31441 gacagcaggt ttccagcccg aagaactaat cattcttgct gcacgtccag cggtaggtaa
    31501 aacagcgttt gcattgaata ttgctcaaaa tgtaggaaca aaaacagatc gttctgttgc
    31561 gatatttagt ttggaaatgg gggcagaatc tcttgtaaat cggatgcttt gtgcagaagg
    31621 ttcgattgag gccagccatt tacgtacagg tcagctttcg gaagaagaat ggtcgaatct
    31681 gatcgttgcg atggggagtc tttctcgagc aaatattttt atcgatgata caccagggat
    31741 caaaatctct gaaatccgtg cgaaatgtcg gaaactagcg caagaaaaag gcaatctagg
    31801 cttgatattg atcgattatt tgcagttgat tgaaggtaac ggtaaagaga accgtcaaca
    31861 agaagtatca gatatctctc gccaactgaa aaaactggcg aaagagctga aagtacctgt
    31921 cattgctctt tcacagttat ctcgtggagt agaacagcga caagacaaac gaccagtgct
    31981 aagtgatatt cgtgaatccg gatcaattga acaagatgcg gacatcgtag ccttcttgta
    32041 tcgagatgat tattatgaaa gagaaggcgg agatgacgat gatggaaacg agccaaaaaa
    32101 tgacaacgtc atcgaagtca tcatcgagaa gaaccgaagc ggtgcacgag gaacagttga
    32161 actactgttt atcaaagaat acaataagtt ttcgtcgctt tcgccaagag aagaatttta
    32221 aagaagaggt tgtgagcctt gagcaatccc gagagctctc gtaagaagat gcaaggagta
    32281 accgcaggaa cgactagagt acttaattta aacagatctc caaagggatt gtgacaaaag
    32341 tcttgtcaca gtcccttttt ccgaataaac ggtgttcaat cagctgaccc ttggtattag
    32401 ctcagcaaac gtcaaaatat gaggaactat tttgacgttt gtttctctaa tactcaggtc
    32461 aaaacgctga tcttacgacc tcttttcctt aaacggtgtt ctcctgccaa ctcctcggtc
    32521 acaagtcaca atatcatcaa acatgagaag cagttttttg aaattgctcc ttgtgactca
    32581 gagttaaacg gcaggctcac aacctctttc ttaaacggtg ttcaaaagct tgcatctgcg
    32641 gtaatacgcc caatcaaacg aaaaatatga gaagctattt ttgtttggct gttcttatta
    32701 cttgatgcag gacagctttt tcacaacctc tgattcacgg tgttccaaaa gtagcttctt
    32761 cgaaaaataa gtcgaaattt cacaaaaatt tgagaagcaa ttttcgtaca ttccttctta
    32821 tttcttgaag ctgaccactt ctgttacaac ctctttttta ataaccattc taaaaagata
    32881 aagttcgttt tttaagcgtt tttataaaat aaaaataaaa atcattcgat tttatttgaa
    32941 atatgtatct attattgata caatggttca ggattaaata atatagaacg aggtgttcac
    33001 atgtcatctg tagtagtagt aggtacgcaa tggggcgatg aagggaaagg aaaaatcaca
    33061 gactttctca gtgaaaatgc tgaagtgatt gcacggtacc aaggtggcga caatgcaggg
    33121 cacacaatta agtttaacgg tgtgacttat aaactgcatt tgattccttc tgggattttt
    33181 tataaagaca agatcagtgt gatcggaaac ggcgttgtag tcaatcctaa atcattggtt
    33241 aaagaattag cttatttaca tgaaaaaggt gtagaaacaa gtaatttgcg tatttctgac
    33301 cgcgcgcacg taatcttgcc ttatcatatc aaattggacc aattacaaga agatgctaag
    33361 ggcgaaaata agattggtac aacgattaaa ggaatcggac ctgcctatat ggataaagca
    33421 gctcgtgtag gtatccgtat tgcagactta ttagataaag aaatttttga agaacgtctt
    33481 cgtttgaatc tagatgacaa aaaccgtcaa tttgtaaaaa tgtttgattc agaaccactt
    33541 gattttgacg atatttttga agaatattat gaatacggac aacaaatcaa acaatacgtc
    33601 acagatactt ccgttatctt gaatgatgct ttggatgcag gcaaacgcgt gttgtttgaa
    33661 ggcgcacaag gtgtaatgct ggatatcgac cagggaactt atccttttgt tacttcttct
    33721 aacccagtag ccggtggggt aacaatcgga agcggtgtag ggccttcaaa aatcaataaa
    33781 gttgtcggcg tctgcaaagc ctatacgtca cgtgtcggtg atggaccatt tccaactgaa
    33841 ttatttgatg aaacaggaga acaaatccgc gaagtaggac gagaatacgg tacgacaact
    33901 ggtcgtccgc gtcgtgtagg ctggtttgat tctgtagtta tgcgccattc taaacgagtt
    33961 tcaggtatta ccaacctatc tttaaactcg atcgatgtat taagtggatt ggaaacagta
    34021 aaaatttgta cggcttatga attagatggc gaactaatct accactatcc agcaagcttg
    34081 aaagagttga accgctgcaa accaatctat gaagaattgc caggctggtc agaagacatt
    34141 acaggatgca aaacacttgc agaactgcca gaaaacgcac gtaactatgt acgtcgtatc
    34201 tcagagcttg ttggtgtacg tatctcaaca ttttctgtcg gacctgatag aacacaaaca
    34261 aacatcttag aaagcgtctg ggcgcaaatc taaggataaa aacgacgact gaatcaggac
    34321 gttcttacca gagcgagtcg aagtgtttat ctatacgata ataataactt gaagaggtag
    34381 tgaaagagac gtttagcctt attgccgata ggctgtctct tgaacactgt ttattcagaa
    34441 taagggactg tgacaaaaac cgtgtcacag tccctttttc cgaa
//
LOCUS       DACGLW010000021        34374 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.021, whole genome shotgun sequence.
ACCESSION   DACGLW010000021 DACGLW010000000
VERSION     DACGLW010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.021"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            complement(<1..900)
                     /locus_tag="IXT53_001071"
     CDS             complement(<1..900)
                     /locus_tag="IXT53_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4904915.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEK"
     gene            complement(1135..1410)
                     /locus_tag="IXT53_001072"
     CDS             complement(1135..1410)
                     /locus_tag="IXT53_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008214712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HAQ4904916.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(1653..2963)
                     /locus_tag="IXT53_001073"
     CDS             complement(1653..2963)
                     /locus_tag="IXT53_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HAQ4904917.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(3145..4374)
                     /gene="rpsA"
                     /locus_tag="IXT53_001074"
     CDS             complement(3145..4374)
                     /gene="rpsA"
                     /locus_tag="IXT53_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HAQ4904918.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(4509..5189)
                     /locus_tag="IXT53_001075"
     CDS             complement(4509..5189)
                     /locus_tag="IXT53_001075"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HAQ4904919.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTREVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(5238..5873)
                     /locus_tag="IXT53_001076"
     CDS             complement(5238..5873)
                     /locus_tag="IXT53_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ4904920.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(5939..7342)
                     /locus_tag="IXT53_001077"
     CDS             complement(5939..7342)
                     /locus_tag="IXT53_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HAQ4904921.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(7329..8360)
                     /locus_tag="IXT53_001078"
     CDS             complement(7329..8360)
                     /locus_tag="IXT53_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4904922.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            8391..8612
                     /locus_tag="IXT53_001079"
     CDS             8391..8612
                     /locus_tag="IXT53_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HAQ4904923.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(8777..9514)
                     /locus_tag="IXT53_001080"
     CDS             complement(8777..9514)
                     /locus_tag="IXT53_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HAQ4904924.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(9667..11007)
                     /locus_tag="IXT53_001081"
     CDS             complement(9667..11007)
                     /locus_tag="IXT53_001081"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HAQ4904925.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(11244..12767)
                     /locus_tag="IXT53_001082"
     CDS             complement(11244..12767)
                     /locus_tag="IXT53_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HAQ4904926.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(13145..13756)
                     /locus_tag="IXT53_001083"
     CDS             complement(13145..13756)
                     /locus_tag="IXT53_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ4904927.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(13879..14004)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(14086..14802)
                     /locus_tag="IXT53_001084"
     CDS             complement(14086..14802)
                     /locus_tag="IXT53_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAQ4904928.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(14808..15377)
                     /gene="scpB"
                     /locus_tag="IXT53_001085"
     CDS             complement(14808..15377)
                     /gene="scpB"
                     /locus_tag="IXT53_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HAQ4904929.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(15361..16158)
                     /locus_tag="IXT53_001086"
     CDS             complement(15361..16158)
                     /locus_tag="IXT53_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HAQ4904930.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(16128..16499)
                     /locus_tag="IXT53_001087"
     CDS             complement(16128..16499)
                     /locus_tag="IXT53_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAQ4904931.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(16518..17405)
                     /gene="xerD"
                     /locus_tag="IXT53_001088"
     CDS             complement(16518..17405)
                     /gene="xerD"
                     /locus_tag="IXT53_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HAQ4904932.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            complement(17501..17974)
                     /locus_tag="IXT53_001089"
     CDS             complement(17501..17974)
                     /locus_tag="IXT53_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ4904933.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(18079..18942)
                     /locus_tag="IXT53_001090"
     CDS             complement(18079..18942)
                     /locus_tag="IXT53_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HAQ4904934.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(19138..21237)
                     /locus_tag="IXT53_001091"
     CDS             complement(19138..21237)
                     /locus_tag="IXT53_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HAQ4904935.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(21457..22566)
                     /gene="rpoD"
                     /locus_tag="IXT53_001092"
     CDS             complement(21457..22566)
                     /gene="rpoD"
                     /locus_tag="IXT53_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HAQ4904936.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(22590..24431)
                     /locus_tag="IXT53_001093"
     CDS             complement(22590..24431)
                     /locus_tag="IXT53_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HAQ4904937.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(24613..27006)
                     /locus_tag="IXT53_001094"
     CDS             complement(24613..27006)
                     /locus_tag="IXT53_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ4904938.1"
                     /translation="MFLILEHARLKHVIIFESWRKMEKKKMNGLTQQEVVQKMASGQQ
                     NDYQEDASKSTKQIFSDNILTLFNFLNFGIGICLLFVGAYSNMAYLAIIVVNIVIGIY
                     QEIHARNLVRKLSIVSQSEVLVIRDGKEQSISTKELVLGDLVKIGAGEQIPADLQVIS
                     GRAEANEALLTGESDLIVKETGAELLSGSFLTSGSVMAEVIRVGADNYAVRLTSEAKT
                     HKAIRSELVDSIRKVSKFTSFVIIPLGIILFLEALFIRDASIKISVIASAAALLGMLP
                     KGLVLLISIALTTGVTKLAKKRILVQDMYSIETLAHVDTLCLDKTGTITEGKMSIQRV
                     EALHPLYEKAIPEIMGTYLAESSDNNITMRAMRDYFELSNRYDVTNKVAFSSDRKWGA
                     MEFPELGVVYVGAPERLVEEDRLPDSLKTAQANGYRVLMLGIAPNQQLNEEKPAFVEP
                     VALFEIDDPIRKNAQETLAYLHEEGVDLKVISGDNPFTVSNIARRAGLPNYDAYVDLS
                     QITEEMEVREAAHRYTVFGRVSPQQKKLLVNELKESGRTVAMTGDGVNDVLALREADC
                     SIAMAEGDGATRQIANLVLLDSDFTTLPEVLFEGRRVVNNVTKVSGIFFIKTIYSFIL
                     SIICAVTAIGFPFIPIQITLIDLAIEGYPSFFLSFEQDKRKITYRYLPTALINALPNA
                     LLVVVNIIAVYLLGKTFAWGNAETTTLMYYLLIGISCMAVVKACYPFNPLRIFLAVTT
                     IVGIYVAAMLFHGILEVNLGLGESVGYFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(27113..27973)
                     /locus_tag="IXT53_001095"
     CDS             complement(27113..27973)
                     /locus_tag="IXT53_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HAQ4904939.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(28032..28664)
                     /locus_tag="IXT53_001096"
     CDS             complement(28032..28664)
                     /locus_tag="IXT53_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HAQ4904940.1"
                     /translation="MKRKRLSIIRILFYMIILTVIGAVLFLGGRTYRSVKRVESYQIE
                     ITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSESLHGEAEIIDNPADSIDQGVK
                     YLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHGGENTVELAEAYSKDVLSPLLG
                     NEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKMNEKIIETYEKVFK"
     gene            28873..29592
                     /locus_tag="IXT53_001097"
     CDS             28873..29592
                     /locus_tag="IXT53_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ4904941.1"
                     /translation="MKKEKKYAKMPDGSEIYYEKSGQGFPLFLLHGNDGSGRFFSEQV
                     PVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEKIDQADFLGFSDGAN
                     LALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVWLLSLFRPSLRKNLL
                     VIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKTIPKASFVLVKEQGH
                     ELARKDPERFNREVLQFLSET"
     gene            29618..32209
                     /gene="mprF"
                     /locus_tag="IXT53_001098"
     CDS             29618..32209
                     /gene="mprF"
                     /locus_tag="IXT53_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAQ4904942.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(32397..33584)
                     /gene="tuf"
                     /locus_tag="IXT53_001099"
     CDS             complement(32397..33584)
                     /gene="tuf"
                     /locus_tag="IXT53_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HAQ4904943.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            33952..34308
                     /locus_tag="IXT53_001100"
     CDS             33952..34308
                     /locus_tag="IXT53_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ4904944.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
BASE COUNT         9588 a         7270 c         5929 g        11587 t
ORIGIN      
        1 tttttccaaa tactccatct ttgaaatctg ttccagccag tggattggat agccaagcaa
       61 gtccagccac acaaaaaagt tttttagttg gaaatcaaaa ggatatgctt ggagattcca
      121 cacgaaatga aacatcattc ctaaaaattt tttcatacta gcagaaagta gatggccatt
      181 ttcggtcaga taaaaagctt gttcaaacag tttaccataa tttgacaggt tttctgcctc
      241 taattgataa aaatagatca tttcttcgat catcccggca aacggccgag tcgttaattg
      301 ctttttattt ggatagtttt tatacagatt ttgcaaaaaa cgatgatcac aaatcattcc
      361 gcaacagata aaagtcaaaa agtcaatctg ctttccgtcc acctgccgct cgctgattgt
      421 ttgatcataa acgatgcgtt ctggcagatt gatggtttgc agcactacac gatttggtag
      481 ctgcaaaatc gtccgctgcg gtgttaatgc tcggcttaga gaacggatcg atacagggat
      541 catccatagt gatgaatcaa gggttgtatg tttttgaaaa aaacctgcca gatccatgat
      601 cccttcattt ccaaatccga tgataaacat ggattgattt tcgggatagc gtttgacgaa
      661 atcaagcagg ttctttaatt ctgttaaatg attgcattga gtgttggcac aaatatacca
      721 gtcattgttt gcttgatttg caaataacgg gttgatctta tctgcaaaca aatcataata
      781 gcgctgattc gttaaaaata agattggctg atcaatggaa agttctctta gctgactgac
      841 tagcgtttct ccgtaaaaca ctttcgtcaa cgcatcttct acttgataat caagttccat
      901 acgcatccct cctcttctta tttttatttt accattagta agcaaattct agaaaagata
      961 agagcaaagc tcacctcata acttgcaaat aaataaaaat taaataaaat acatgtgttt
     1021 tctatgtatg acatacggat tcggaatctc tgtctcttat ttattccttt tctgctagga
     1081 aataaaaaga aaagcacttg tagccctaga gccacaagcg ctttttcaac tgtcttattt
     1141 aacagcgtct tttaatgctt tacctggttt gaatgcaggt actttgctag ctggaatttc
     1201 gatttcttca ccagtttgtg ggttacgacc tttgcgagct gcgcgttcgc gaacttcaaa
     1261 gttaccaaaa ccgattaatt gaactttttc gccgttagct aaagcatctt gaatagttga
     1321 aaatacagca tctactgctg cagttgcatc ttttttagtt aaatcagtag ctgaagcaac
     1381 tttttcgatt aattctgctt tgtttgccat ggatagattc acctcctcca caagagatat
     1441 gatagaaata tcatttagtt aaaactttct gagtggtcca gaagtttaaa aagaaatatt
     1501 gattaacttc aatattctgt tatcacgata tcatatgaac cgcttaatag caaggttttt
     1561 accaattttc acatgctttt tttagtcata gaaacgtaac aaacgaaact gtctttttct
     1621 tttgaaaaaa aaacgtcgat atggtaaaat gcctattttc tcctacgagg gataattcgg
     1681 atggacgtcc cttcaaaagt aaatgctttt ctgatttgat tttctaagaa acgttcataa
     1741 gaaaaatgca tcaattcctc ttcattgaca aaaacgacaa atgtaggtgg tttgacagcg
     1801 acttgtgtag cataaaaaat cttcaggcgt tttcctttat ctgttggtgt tggattgatt
     1861 gctacagcat ccataatgat atcattcaac acagccgaag agatacgaag attctggttc
     1921 atacttactt gttcgatcat ttcaggcaga cgttccaacc gttgtttagt cactgcagaa
     1981 acaaaaacga ttggagcgta atcaagatac cggaattcat cacgaatctc ttgttcgaaa
     2041 tcacgcattg tattggtttc tttctttaca agatcccatt tattcacgac gataataatt
     2101 ccacgaccag cttcatgggc gtaacccgct actcgcttgt cttgttcccg aatcccttct
     2161 tcagcattca gtaccatcaa aacgatatca gaacggtcaa tggcacgcat tgcacgcatc
     2221 acgctgtatt tttcagtatt ttcgtatact tttccgcgtt tacgcatacc agctgtatct
     2281 atcattaaga atttctgacc actttcagaa acgaagtggg tgtcgattgc gtctcgggtc
     2341 gttccttcaa tatccgaaac aattacacgt tcttctccta aaatcgcatt gatcaaagag
     2401 gatttaccaa catttgggcg accaatcaaa ctaaacttaa tcgtgtcgtc gtcctcttct
     2461 tcagtctctt cagagaaata cttcactgct tcatctaaaa tatcaccaat ccctagtcca
     2521 tggcttcctg aaacaggata aggatcgcct agccctaagg aataaaattc atagatatcg
     2581 tttctcattt ctggattatc tactttattg actgctaaaa tcactggttt attacttctg
     2641 tataaaattt tcgcaaccat ctcatctgcg tctgtcacgc cttcgcgtcc gctgactaca
     2701 caaatgatta catctgcttc ttcaatcgcg atttctgctt gatgcttgat ctgatccata
     2761 aaaggttcat cgccaagatc aattccgcct gtgtcgatca cgctgaattc tcgacctaac
     2821 cattcacctt ttgcataaat acgatcacga gttactccag gagtatcttc gacaatcgag
     2881 atacgctcac cagcgatgcg gttaaaaatc gtagacttac cgacgttcgg gcggccaacg
     2941 attgcaattg ttggatttgc catattattt cctcctcaca agtttccaat ctcacttagt
     3001 ttaacgaatg cattgccaac tttcaagcat agattgtcaa ttaaacgaag tatttttatt
     3061 ccttattgaa atactaatcg cttttcaaaa gaaaaactga gacaaaagca gctgctttca
     3121 tctcagtcac agatacaaaa tcaattattt ttcagaatca tcggttgctt tttcttctac
     3181 ttcttctttc aacgcatcac ctaaaatgtc tcccatagta aatccagtat tttcttctgg
     3241 taattcataa gactcaacat tttttggttc ttcttctgaa gctggttttt cttctaaagc
     3301 tttgatactc aatgcaatac gatgttcttc tggatggatc tctaatactt tcactttcac
     3361 ttcgtcacct tcatgaagca cttcatgtgg tgtcgcaata tgtttatgtg aaatttgaga
     3421 gatatggact agcccttcta ctccagggaa cacttcaaca aaggcaccga agcttgtcaa
     3481 acgtttaact gttccgtcta atactgtgcc aactggagct tttgtatcga tttctgacca
     3541 aggtccaggc tgtgtgtctt tgattgacaa ggaaacacgt tcttgttcag gattgataga
     3601 caacactttg acatttacat catcattgac agccaataca tcacttggtt ttgctacatg
     3661 actatgtgaa atctctgaga catggactag accgtcaatt ccgcctaggt cgacaaatgc
     3721 gccaaaatca gtaagacgtg caacacgtcc actgagaaca tctccctcat ggatagttga
     3781 aagaatttct tttttctttt cttctctttc gttttccact accgctttat gcgataagat
     3841 caaacgattt tctgatggtt cgatttcgat gattttaaag ctcaatgttt tgcctttata
     3901 gtctgaaaag tctgcaacaa aatgatcttc caccattgat gcagggacaa aaccacgtac
     3961 accgacgtca acgactaaac cgcctttgac aacatttgta acaggtgctt cgatgacctt
     4021 gccctcttgg aaatctcttt cgatatcttc ccaaactttt ttagcatcta aacggcgttt
     4081 tgaaagtaga tagctgccgt tttctttatc tttgccgatc gtactgataa cgactaaatc
     4141 taatacatca ccgactttta ctacttcgct gatatcttct accggcaaag ttgataattc
     4201 ttttgccgga acgactcctt caactccagc cccttcgatt ccgacaatca cttgtttgtc
     4261 ttcaatgact aaaacttcgc ccttaacaac atcgccaact tttacttctt gtacgctttt
     4321 cattgcgtct tccattgact ggttgttctc ttgattttga tcaaattctg tcataacact
     4381 tgtcctccta accaacattc tgccgtaagt acggtactgt ttttagttta attgattatt
     4441 caaaaaaaat aaagcgattt cgttcgtttt ccttgaaaaa acaattgttt ttcatttttt
     4501 ttcttaattt aaaaaagaag ataacctttt ttcaaaacaa cagcttttat tgctgcaacg
     4561 acttctggaa tcgatttgcc agtcgtatcg atacgaacag catcctctgc ttgcttcagc
     4621 ggagagactt cccttgttga atcataatgg tctctttcct gtatctcttt cgtcagtgtt
     4681 tcaagatcag tcggaattcc tttttcttgg ttctctttga aacgtctttc tgcacgctca
     4741 cttacgcttg ctactaaaaa gattttcact tctgcattcg gtaatacagc tgtcccgatg
     4801 tctcttccgt ccatgacaac gccaccgcgt tcccctattt ttcgttggac agcaactaat
     4861 tcttctctga cttttccatg tgcagagact tcagaaacag cttttgtgac atctggttga
     4921 cggatttcta aagtcacatc ttttgatgct atgaatacat gctgtccatc ttcttgttgc
     4981 gaaaaagaga taggatactt tttgatcagc gaaactagcc cttcttcatc tgaaaaagat
     5041 acattgttcg taatagctaa ataagttact gcgcgataca tggcaccggt atctgtataa
     5101 atataattca attcttttgc taatatcttg gcaacggtac ttttcccaga tgaagcaggc
     5161 ccgtcgattg caatactgat ttgtgtcatg atataactcc tttttctcag aaaaaaagcc
     5221 gcaaagcgcg gctattatta tttaattctt aattcttgac cagggtaaag cataaagttg
     5281 tttggatcta agccattcaa ttcaaacaat tgatcagtgg tgatccctgc acgctcagcc
     5341 acttgttttg gaccttcgcc tgcttgaaca gtcgttgttg aaccggctgc agctgatgaa
     5401 gaaggtgttg tttctgctgc attgctgctt gaatcccctg cttgataagt tgcctcactt
     5461 gaggaagtcg gtgttgtctc tgctgcaata ctgctttctg acgttacacc tgtatcttga
     5521 gacacagtag attcttccgc gattgtacta gattctactg ttgaagtctc agttgactcc
     5581 tcttttgttg tacttgtaga actttctact actgatgtcg aagtttcaga agaagttgct
     5641 gctgttgcat tgttgcttga tccatttctg atccagaaat aagcacctgc tggaatagcg
     5701 atacataaga ccagtaaaat caaaaatagc gaaagaaata atgtatttcc tttcttttgt
     5761 tgtctctgag aacttcttga tgaaacttca tcatctgtat cgtagattgg ttgttcccat
     5821 ggttcttgtg tttcgttatt ttctgtagat tgatgtctat cttttctgct cactgtttta
     5881 cctcctaaac tctatcgtgt gtattatacc ataggaaaaa gtgtcttgtc ctaaaaaatc
     5941 aatgttccat gtttttttta aataaattta gtaaaatcgt ttcccaatga agttgttcag
     6001 tcttagaaaa aagcttcctc tttggcagaa gttctgcttt tgctccatca atatcacaac
     6061 aattctgcgg cttttcggag agtttttcgc caaaataagc taagatgaat tttctgcggc
     6121 agttttcttc attaatatag cgaagcatct gctgcaggcg gaattctttt tctttttcct
     6181 gacgtttgag ccgttccacc caattttctg gtttttcaga ctgctggatc agctccatcc
     6241 attttttttg cagctcatca aagggagctt gctctgtggc atattccaaa taaagctcaa
     6301 aactttgtcg ctgttctcta gaaagttgat taaagaaata atggatacgt tcatccccac
     6361 tttgataaaa aagaattgct gcactttctt cctgatctct gcctgctcga ccgatttctt
     6421 gtacatagtt ttctagactg tctggcaaat cataatggac cacataacgg atatcagatt
     6481 tatcgatccc cataccaaat gcattcgtag caatgagaaa ctgcagctga ttttttacaa
     6541 actgctgttg aagttgtctt ctttgagctg catcaagacc accgtgatag tatccaactg
     6601 taaaacgttc cctgaataaa tgatacaatc gttcgacttc tttttttgtt gcacaataaa
     6661 tgattgccgc tccttgtgct ctttccatga agtgttcaag ttcttgttct ttttgctgcg
     6721 ttttccgaac aaaaagcgag atattttgcc gattgactga ttgtctgatc tcttttggtg
     6781 gtcgatcaaa tagtacatga cgtatatctc ttgctactaa atctgttgct gtcgccgtta
     6841 gtgccagtgt gaccgggttt cctaaaagct ttcggatttt tcctaactgt tgatattctg
     6901 gacggaaatc aactccccat tgagacacgc aatgagcctc atctactaca tataacgcaa
     6961 tattttgttt tttcagatgc tctaaaagag aaggattagt caacatttcg ggactcaaaa
     7021 acaaaaattt atattgcgat agatgacgga ggacgtatct tctttcagat tgcgaaagta
     7081 aactattgaa agcagctact cgtttctccc ctcgtttttg taattgcgtc acttgatctt
     7141 ccattagaga aagcagcggt gacacgatga tgaccagacc ttccattaaa taccccgtca
     7201 attgatagca aaggcttttt cccgttcctg taggcaaaat agcgagagta tcttctccat
     7261 caagcaacgc ctgaataatt tcttcttggc cgggacgaaa tgaatcaaat ccaaaatatt
     7321 tttttaattc atttattaac attctgtatc gcttcctttc tcaatagata gatttgatac
     7381 aaacgaaatt ctccataatc taattgtcca tctacattcc attcgctaaa gcgccactct
     7441 cgcacggaat ccttatttgg ttccaaacgt tcgattgttt ctgcagagag aatccactcg
     7501 tatggaaaat catcaaaaaa caaggcccac tcaataagat gatctgttac tgttcctctt
     7561 tttaaatgac ggattgccat gatctggtca attgtctgac cattcataaa catctgcttg
     7621 gtgatcatca tgctttgatt gaaattttgc tgtaataatg aatcgatcaa agcgtgccaa
     7681 agcgaatctg gccgtttttc gatttgtgct aacaaaaggt ggatacatct gctttgttgc
     7741 agataaaccg ctgactcatc attgtttttt gccagctgat aaggaagcag ccctgttcgc
     7801 tcatttccag ataattggtt agctaaaaaa tctgctgctt cttctggtat ctccgaaaac
     7861 agctgcacca aactctcata cgcagtatca atcaaaattt cacgtggcaa ttggctttca
     7921 ctaagccatt tttttagttg gaaagtataa aaaggacttg tctctgctgg aagatagtct
     7981 tgaatacccg ttgccagatt agaaattact tgaacagcaa atttaatgag ccgccaacta
     8041 gtttcccctg ttctgccata gcggtcaaaa cgcaaaccag tatgttctat ttgatgttcg
     8101 gacaacatat cagcccctgc aggcgttagc tttgcttcat tttcatttat ttcgatccaa
     8161 ccttgctgcg caattttttg caaaatttga taaaacttat cctgcttcaa ttctggaaaa
     8221 ctgttatgga taaacaaaag ttcgtgggta aatccataaa taagtactga agtcgttcgt
     8281 tttcccacaa ggagttggta aagagaacta atccgcagct tgtgtcctga tgaaaatagc
     8341 gataaaataa aaacaatcat attattctcc ttaagttagg atggttagaa atgtcactac
     8401 tatgtaaact tgtacctgaa cgctgtattg catgcggtct ttgtcaaatc gaagcgccag
     8461 atattttcga ttatgatgaa gaaggaatcg ttttatttgc ccaagaacca gaggctcatc
     8521 aacagttcgt gatgccggaa gaagaaacat atgtaaaaaa agcgtatcaa aaatgtccag
     8581 ttcgagcgat tttattagaa aataaaatat aaatataaac gaaactttaa aaacgtacac
     8641 taagttctct aaacttgaaa ataagccggg atctgtttct cttgttccgt gaaatcccga
     8701 cttattttga aagattcatt tgctatctat agttaattga ctagtttttt cacggaccct
     8761 ataggaccca tttatattat ttcaactttt tcagccagtt ttccaatgta ttgacgatca
     8821 cagtttgctg gtctgcgtta gaaatttctg cctctccatc tcctttttgc tcaccatagc
     8881 tgccaaaacc tccatgattt ccgccattga ttgatacata ctctgttctt tccggtaaat
     8941 actgcttagc ttcttgataa gcttcctgat tcaatacttt atcttttgtc gccgtgatcg
     9001 ataaaacagg aacagctgat tctgtcaatg ttcctttttt gtcgggataa cttgccatga
     9061 agaatacccc ttttaaatgt ttgtctgaat gattcttatt gacataacga cttgccatta
     9121 cgccacctaa tgaatggcca ccgatcacat aatcggaatt tggaaaatct tctttgacct
     9181 tatccgcttt gtttccgccc atcactgcta gatcaagtgg cattttgaca atagcgactg
     9241 aaaagccgtt ttttgctaaa tcttgcgccc aaatactata actttctggc tctacgagag
     9301 caccaggata aaaaataagt aatggagact ttccatgatt ctttttctct ccttcaaaaa
     9361 ataagtaatc ttctttgttt tttccttctt ttaccaacac ttgcgcttct gacgatggtt
     9421 cgtacgtatt ttgctgtata tagtaataac cggctccacc gagtaatcct aaaatgatga
     9481 ctaagccaat tagtattcgt ctaaatattt tcactttctc ccaccttttc tatctgagtg
     9541 accccttttt cttaatcaag tatgattata ccttaaaaaa ataagagaga aatgcaaact
     9601 ttccagcatc aaataaaaaa agacggaata tccatttgaa tatccgccct ttttgtatgt
     9661 ttatttttat ttagcacata aacgatacac agcttctgca taaatttcca ttgctttata
     9721 catactactt aattcccatc gttcattcgc ctggtgcata aagtcaggag tatcagggaa
     9781 catcgcaccg aatgccacac actggttcat cgtacgagca aaagtagcgc cgcctgatac
     9841 aaacggttca gtcatgtctc cagtaatatc gcgatatgtc cccaatagat ttttcactaa
     9901 ctcactgtct agtggcacgt ataatgaatc aaggaagtca aattcttcat aagtcaaccc
     9961 atatttttct gctgttttag ttaatttagc tactaaatca tctttcttga atgtaacagg
    10021 gattctcata tctacaccga tttttgtttg ttctggtgtg atttccaaac ttgcaaagtt
    10081 catcgttagc tcgccggatt gttcatcttc tgttttgccc acgacagctt caccagttgc
    10141 attttcttga actaattttc ctaagaaatt gattgggcca aaatcaaata cttctgaaag
    10201 agcaattgcc agacgagaaa tagcattcac tccttgtgct gcatctttcg cgtggatact
    10261 tttaccaaga acgacgatgc cttcaccttg ttcttcgaaa tcgaattcgt gttttttcaa
    10321 tgcttctttt acttcagaaa gcttttctcc agagtaagga gctgcatcag gaacaacatt
    10381 caacgctcct ccagctttta cagaaaactc gttcgttcct ggtcctgtca agtaagcttg
    10441 caataagcct ttttctgcat aaattaaagg aaattcagca tctggcgcaa atccttgcgt
    10501 aattccttct tctttttcgt tatatttttc taaacaacgc cataaatttt cttcatctgt
    10561 accaaagata aaacgaatac gtgtcttgaa ttccacgcca gcatcaatca aagctttgac
    10621 ggcatacatt gccgcaatag aaggaccttt gtcatcttgg gatccgcgac caactaacca
    10681 gccgtctttt atagttggat cgaatggttt tgtttcccaa ttattttcgt cccctgctgg
    10741 aacaacgtcc atatgacaca agataccgaa taattcatct ccagagccaa tctctgaata
    10801 accgtaatag ccttctggat ctttaaatgt tctaaatcct agcttttcac tgatttccaa
    10861 tactttatcc aaagcttcaa tcactttttt accgaaggga tgtccttgtc ctgaatctgc
    10921 ttcatctagt acggaaggaa ttcggatcaa ttcgctcaat gattctaatg cttgttcatg
    10981 atgttgttct gtcacaaatt ttttcatgat attatcctcc taatatgtta aaaagggtca
    11041 tgtcaaattc ctccgacaca acctcttctt ctcaaataat gacgttttaa gcctgctcca
    11101 tcagcaagca gaatgttctt aacaaatgat gaaagaaaaa acactcactg aatggagcta
    11161 ttgacttatc cacgtgctta tcgatagccg tttatcggtt tcttcattct tatgatagac
    11221 cttgtacctg tatttgtcta ggtttagaac attgctgcta cacctaggat cacacagatg
    11281 gctacaaata atatcgcaat taatttaccg gtaaatttcc accacactga aacatcgaaa
    11341 cgagcaattg ctacagcacc cattacgata gcagatgttg gtgtaattaa gtttactaaa
    11401 ccagatgcag attgataagc agtgattact aaatccttac cgacgccggc aaattcaccc
    11461 ataggtccca tgattcccat tgttgcagca gctaagccgg aagttgaagg aatcaagaat
    11521 gacataggga tatagaaaat aaatgtaata atgatgaata cacttgaaga taatgaactt
    11581 aatccttgtt cgccccaatg taagatggtg tctgtgatta atccattgtt cataaccact
    11641 tggataccac gagcaacagc tactacgatt gctacaccga tcaaatcaga agcaccagct
    11701 aaaaattcag agacaaattc agactctttc atgccataag tgattgctac tacgatggac
    11761 attactaaga ataacatagt aatttctggg aagtaccagt cacctaatgg aagcatgctt
    11821 ttacctagaa ccgtacctag taccggaagg tttgttaacc agtttgtcaa tgattcaaaa
    11881 atcgtgaaat tcgcattcaa ggttgtccaa ggaatcaaac tgatgatcat gataccaaaa
    11941 gtaataaaga acaatacatt aacatgtttt tgttttttcg tgattacttc ttgacgaccg
    12001 attgcttcaa tatcgaaatg cttcatatct tctttacgtt ggttgtatac taatgatttt
    12061 gttggatctt tttcgatctt tgatgcataa cgatacacat atacaataga aatagctaac
    12121 aaaataacga acatcaagaa tcgaagacca ataccttcac ccatcgaaat cccaacagct
    12181 tgtgaagcta cgcctgttgc aaaaggattt accgttgaag ctagacagcc tacttgagac
    12241 cctactaata cgattgctac agctactact gtatcaaagc ctacagccat catgactggg
    12301 atcagtaaag catagaatgg aatcgtttct tctgccattc cgtaagtaga gccgcctaat
    12361 gcaaataaca gcatcaatac tggaataagc attttttctt ttcctttgaa tttcttcaca
    12421 actgaagcaa tcccggcatc tagtgcccct gttttgttga taatacctaa aaatccacca
    12481 acaaccaaaa tgaagaaaga gattgggatt gcagcaggtg tttctttagt tagctccccc
    12541 gctggtacac ctagcatccc attgattggc gccatgaaaa tatcccacag cccttgagga
    12601 ttttgttgta ctcgttcata tgatccagcg acgatgttgc ctgcatcgtc tacactatag
    12661 tgcccagctg gtataaacca agtaagtaca gcgattactg caataataat aaataagacc
    12721 gtatacgctg aaggcatttg aaattttttc ttttttgttt gttccatctt cttcttcctt
    12781 tcttttccgt tagagacaac aattttggct ctaatgttat tttatcaaaa agagtatcag
    12841 aacaaaaata ataacacgca ttttgtaaaa cttttgttca aaaagttcgt ttttccctat
    12901 tccctcctct tttttcaggt ttttaaagct ttcttctttt tcattgtagc gtttcctttt
    12961 ttttataaac aaacattttc gtcttttaaa aggagaagaa tttcacattt gcaaagaatt
    13021 gattgatttt tatgcataaa tcaaaaaaat actcaaaaca aacaaataaa acgtttacat
    13081 tattgcatgt cttgtatttt tttccaaaaa aaaagaccaa gaaatttctt gatctttttg
    13141 atatttattt gactgctgta tgacgttgtt cgaactgatg ttgttttcga cttaaccatg
    13201 gaagaagttg cgcatgcagc actaaaaaga cagcactcac gatgaatcct ttgatcaagt
    13261 tgaaaggaac gatcccaatc agtacatagt ttgctaaaga catgcccagc atctgtgttg
    13321 cgttcagccc taaaaacatt aagtaaagag gcgtaataac gaaataattt gcgatactca
    13381 taaaaatagt tagagcgatg gttcctgtaa tgacacctaa aaatttgttt cttcctttat
    13441 gctgtttctt tacactaaag aaataataaa tcgggaatgt aaagatcgtt gttgataaga
    13501 aactagcagt gtctcctacc aaattatccg gagaaaaacc tgtagtcagc agatgtaata
    13561 ctgaacgcaa gaacgctgtt gcaactccgg ccgctggacc gaataagaac atgctgatca
    13621 ataccgagat atcactaaaa tcgatcttca aaaaactaaa agccggcatg atcggaaaag
    13681 caaaatactg taaaactaat gcgatcgcag caaacatcgc tactgcaacc attttctgta
    13741 cacgggtgtt cttcatttga aatcctcctg ttaggactcc tcttcagcga actttttgac
    13801 gtttctctcc aaatgaaaaa actctatttt ccagggaaaa tagagttaga gttatttgat
    13861 caagcgtcgt caaataagct ctatcttctt tatccagact atactgtcgg tatcggaatt
    13921 tcaccgattc agctattttt ttgcaaaata ggtcgtggac tataaccacc ggtcgggaat
    13981 ttcaccctgc ccctgaagac gaaccttata taatttttgt ttacatattc attatacata
    14041 tctattcaaa aaacgcaaca tctaacactt ataaaaagta agtgttcatt ttgagtggtt
    14101 cagcaaactg ctgacttctt ttttgttcag ttcacggtaa tccccaggac gtaggccttg
    14161 taaagtcaga tctccgtatt tttcacgttt tagtttttgc acaggaagac caactgcttg
    14221 aagcatattt ttcacttgat gattacgtcc ttcgtggatc gtcaattcca cgatactgtg
    14281 gtttgtctta ggatccactg ataaaatttg gaatttagct ggagatgttt ttcgtccttc
    14341 gatctttatt ccttttgtca aaggtgcaag catcgatttt gtagcgatcc cttttacctt
    14401 tgcaacgtaa actttatcca cttcatgttt tggatgtgtc aggcgctgag caaaatctcc
    14461 atcatttgtc agtagcaaga tccccgaagt atcgtaatcc agtcgaccta caggatagat
    14521 acgttctttt accatcggca aaagatccgt aacaactttt cttcctttgt catctgcaac
    14581 agcagaaata actcctttag gtttgtacaa taaatagtaa ccgtattctt cttgataaat
    14641 tgggacttca tccacttcca cagtgtcatg tttcccaact tttatcccta attcccttac
    14701 aacttgtccg tttactttta cacgtcctgc aaggatgaat tcttctgctt ttcgacgaga
    14761 agtgatgcca gcgtgggcaa ttactttctg caatctctcc attacaatta ttctccttta
    14821 ttcattccat caaagaatag atctggtgac acgtcttctt caagtgaaat ttccatttct
    14881 tgtgtatcag gcagttcttc taatgatttc aaaccaaaat aatctaagaa gtagccagtt
    14941 gttccatata atatcggccg acctggtcct tctactctac ctttttcttc aatcagttta
    15001 tgggcagcta gacgctgtac cgctcctgaa acctgaactc ctcgaatctg ctcgatttca
    15061 gctcttgtga ttggctgttt ataagcgatg attgctaagg tttcgacggc ggcttgagat
    15121 aatgtgtttg atgtcccttg cgcatatcgc ttaagtaagg gagccagctc ttttttagtc
    15181 gttaatacat aatggctttc cgtttcaaaa atatgcaaag ctgagtgcga gttttgagtg
    15241 taagattctt ttaattcttt cactaaactt gtgacttctg cttcgctgag atttaacagt
    15301 ccgcttaatt cttcattact gatcccttcc tcgccaacaa taaaaagaag ggcttcaagt
    15361 tcacttaatt tcgtcatgtt cggtatctcc tactgctgga taaagcataa ttgattcata
    15421 gttttctttt tgttgcgcaa tgatctgccc ttttttcatc aattctagca aagccataaa
    15481 agtagtgaca acttcacttt tagaaggtga ctcaaaaaaa gaatcgaaag tacagccatg
    15541 cgcatttctt tttatacgct cttcgataaa taccattcgt tcttcgatcg ttgtgtcatc
    15601 gggcgtgatc gttttttcca ctttttgaga aaatttctta cgttctaaga ttttctgcat
    15661 ggcacggatc aaatcgattt ttttgacttg tcctcgcttc aacggctttt cagcatccgc
    15721 taacccatcg atgttcatag gttcttttgt gtaataaaga cttcgttcct ctgctttttc
    15781 tgtcagtctt tcagctgcat atttatattt acggtattct aaaagctgag tgaccaattc
    15841 ttctcgagga tcttcttccc ataattcttc atcgtctgtg acgaattctt ggacaggaag
    15901 caatgtcttg cttttgattg ccatcaatgt agcagccatc actaagtatt ctcctgcaac
    15961 agctagctcg agtgttttca ttgtatggat ataattcata tattgctccg tgatcgtagc
    16021 gatcggtata tcatatatat cgatttccat ttgctgaatc agatgaagca gcagatcgag
    16081 aggaccctcg aatacatcta atttaatatt gatctccgtc aactcattca ctctttcttc
    16141 tttttctttg ctagccattt tccaatgtaa ggctctgtga tttcagttgc acggatggag
    16201 acatccgggt acttttcctg taattcatct aacatttgaa agcccatatt ctcaccgcga
    16261 taagacggat tcaaagaaat gtcatggatg atcagctgat cgttcgttgc ccagtaaata
    16321 ccaataatcc cttgaatgtt ggttgaacct tctggcgtaa agaaaaaaag ttcataatcg
    16381 tcttcagatt cgtacgtatc aatctccttc aacaaagaag ttttacctgt cagtttctta
    16441 tggaaactca atagtcccat agcaatttta cggttttctt tccggtaatg tgttaacatc
    16501 ttctcacctt cctatgacta agcccttgga aaatgctgct tataaacatc cgtcattctt
    16561 ttcttcgtga tatgtgtata aatctgagtc gtagagatat ccgcgtgacc taacaattcc
    16621 tgtactgtcc gcaaatcagc tccattttct agcagatgtg tggcaaagct atgccgcagc
    16681 gtatgtggtg ttacttcttt gtaaatccca gcctctcgta ctaactgctt cagatttttc
    16741 cagattcctt gccgagaaag acctttccca tgattattga caaatacatg catttcagat
    16801 gcgtccttta caagtaatgg acgtgcttcg tctaagtagc gttccagcca ttggatcgca
    16861 taatcaccta aaggaatgat ccgctcttta tctccttttc caactgtctg taccagtcca
    16921 agagaaagat gaagatctcc caatttcaat ccaatcaatt cactgacgcg catcccagta
    16981 gcatacatga cttccaagat tgcacgatct cttatcccta acgtttttgt cgtatcaggt
    17041 gcttcaatca gcttttctac ttctgtcaga gtcaatgtac ttggtaattt ctgtgctttc
    17101 ttcggcgtat cgatatgttg catcggatca tgatcagtga tacgctcttg ccgtaaaaac
    17161 tgatggaatc ttctgagact tgagatcatc cgagtaacag ttgctgtcgc ttttttttct
    17221 tcatgcaact tctccagaaa acggatgact aaaaagcgat cgacatcctg ccatgaagca
    17281 attttctgct cctgtagaaa atgcaagtac tgttccaaat cacgttcata gctttgtcgt
    17341 gtattggcgg acaatccccg ttcaatattc aagtagtgga gataatcgtt gatttgctct
    17401 ttcataataa tccccctcat gcctctctat catatcagat accgcttcat tctgatagtc
    17461 tttttccata atctgagcaa agaaaaagcc gtctagacac tcagacggac tggtttcttt
    17521 tttctttttc atagcattcc tgacagattc catggaacgt tagccgatga tcttttacta
    17581 aaaaatgata acgttcttct actacttgct ccacttcaag taaaagatcc tcttctactt
    17641 cctcgatcgt gccgcagttc aagcatagta aatgatgatg gaaatgtttg gcgccttctt
    17701 ttctcaaatc ataacgggct aatccatcat taaaacttac tttatcgaca attttcaatt
    17761 ctgtcaagat ttcaagggtt cgataaacag tagcaagtcc aatttccgga ctcttttttt
    17821 tcactaaaaa gaagatttct tctgcagata aatgctcttt ctcattttcc aaaagcacaa
    17881 gcagagtagc ttctctttgc ggtgtcagct taaaccccgc ttcatgtagc tgctgttttg
    17941 tttttttcat cgccgaaata gcattcatga ccatttactc aaccccaaca atttataata
    18001 attacaaata aaaaaagata taacactatg ttaattaaaa ttattataag ctagttaaga
    18061 aaaacatgca agggattctc aattagattt tttgactagt tttgtcacac cgttttcttg
    18121 tgtaatcaat ccttgtttca aaagattacc taatgcgcgc ttaaatgctc ctttgctgat
    18181 accaaaagct ttcatgattt cttctggatc cgatttatct gtaaagttga tttgctgatc
    18241 tgcagaacgt tctaaaatgg ttaaaatcat ttgtgcatca tctgatatag cttcatatgc
    18301 tcttggcttc aatgaaagat tcagtacacc gtctggtcgg acaccaatca ctcgaccttt
    18361 tatctgctcg cctagacgag gttcattata tcgttcagaa ggatggataa acccaagata
    18421 gaaatcttcc gttaaaacgt acgtacctgt taatttcaag cggtatgctg ttcctatcac
    18481 atctttgttt ttcatttctt ctgttccata tttgcttaaa gatttgaata ttttttcatc
    18541 tgctaaactg ccccaaatac ggtcttttgc atcaactcta agggaaatca tcaagcgatc
    18601 ccctttttta ggccatagct ctgtcattgt cggcaattca tcaagagata cgacaaagtc
    18661 tttatctgga agaccgatat cgacaaacgc acctaaatct tttcgtgtag cagtaacgat
    18721 tccaaaagcg taatgaccga tacggctttt aggaatttgt gtagtgatcc gattttcatg
    18781 tttttgattt tggtaaccga atccttcaac tgcttcaccg attttgtgtt caccttctga
    18841 tttgcttaag ccaaaggttt gtccgttttt ttggacaaag tagttttttt cattttcatc
    18901 aataataata cctgtaaata tagttgctaa taaatcgttc atttttttct ccctctatag
    18961 tgaaaggttt tcttaataca tttctttgct atcataacat aatgttatct acttgaatag
    19021 ctgagccttg gtttggaaag gtgaaaaaag gagtatgaca acagttgtgt catactcccc
    19081 tttttcgaat aaacggtgtt aaacggcagg ctcacagcct ctttcatttg cctaaaatta
    19141 attgctccca tttcctccga accatccagt tttacagttg atggttacga tgtaaaatgg
    19201 caaagcactt tctccaatgc ctttcatcaa tgcagatttt gttgaagtgg catatagatt
    19261 ataatagcct tccccatttt cgattctggc tttttctaat ctgtaacctc cttcaacgat
    19321 atatccacgt tggatacatg tagcaatcac tttgctttgg acacctggtg cccacgtcca
    19381 tgctggatta gatgcatcgg caatatcact tgctctggca gatttgattg gctgattcgc
    19441 tgagtttgaa gaggaagggg tcgaggatgc cgcttcttgc tgtgctgctt gtgcgaccgc
    19501 agcttgttga gcggcttgag ctgcagctgc tgcttgagcg gcttgttttg cttgttcttc
    19561 agctgctttt ttcgcttctt ctgctttttt cttttcctct tcttcttttt tctctttatc
    19621 agtgagcatc ttatctactt gtttcaattg ttcattcaac tgagcagata gatctgtatt
    19681 tttcactttt tctactgctt cttttgccgt cttgtattgg tcgcgtgttg ctgaatccac
    19741 tacttttccg tctttaaaga ctatagctgt tttttcttta gctgtttgca attcagcata
    19801 ttgattttta gcttctgttt gtgcttgttg aatcaatttt ccaaaatctg tgttatccgt
    19861 ttcgattgct tggatctctt tatctgcttt caacttgatg tcatctgcta gatgatcatc
    19921 gacgatggca ggtgctatga accactcatt cactttttgg atatctcgga tcttagattg
    19981 gatatcttgg taagttgttt ctaactctga atatcctttt tcattcttaa gactggataa
    20041 atcatttttt agttttgata aatcttgatg aatcatatct gcacgaataa attgatggtg
    20101 atcatctgta taaaaggcag caagtttctc atgaagttct tccatttgtt gctgtctttt
    20161 tgcttctgct gctacttcgg cttctacttt ttgatgatgg tcataataag cccagcttcc
    20221 tccaccgagt agaagtactg ctgctaaaac gagataaggt actttttttg attttttctt
    20281 ttgcgcttta tctggatctg ttttttctaa gtttgattct tgatgtgttt tttcagtatc
    20341 tggcacgatt gttattgatt tcgcttctgc ttcaaacagc ttaggctggt tctcttcttt
    20401 ttctattttt gcttcaagat cagtaccctc tttgcttaca gattgatttt cagcatcgga
    20461 ttcactattt atttcatcgg gctctgttga ctttttgtct gtgttttcga gtatttcttt
    20521 tttctctgct tccggcagtt tattcgtttt ttccttttct aattgttttt cagtaaatac
    20581 ttcagttttt tctgtgatat ccgtttttac gtcttttatt tttactgttt ctttacctgt
    20641 ggcgttttct ctatcagagt ctttttcagt ttcttgttcc ttttctggct ctactttgtt
    20701 ttcctgtgca attttttcac tgatcatttt gagcagctct ttcgcgtgct cttcttttgt
    20761 atcttcacga tgtgctttga tataagcgga tagtatcgaa ttttcttcct cattatcgac
    20821 gatttctttc agttcagcta ctcttctttt ttgttttgat tcaagcgttt cttcttgatt
    20881 ctttcctgat tcttccgaag tctcatgaac agttgctttc ttctgttcat ttttctctat
    20941 attagttact tgatcgtgag caggtggttc ttcgtcttct ggttctgctt tttcgaataa
    21001 ttccattaca gattctagag gtaaatcttt caattccgac cattcgattg gatcgtttaa
    21061 ttctgtatca ggaaagactg catcttcagt tgatttatcc tgcatagctg catctgtatt
    21121 tgtttcttca attgattctt ttatttgctg atcttctgca gttatttgat gaccgcagtc
    21181 tggacagata gtattctgct ccattttttt cgatccgcaa tttggacatt ttttcatcac
    21241 ttagcgatcg gctcctttac taggtttacg ctaccattta atctatcata ttctatacgt
    21301 cacgccaata gcttattttt aggatttact taaaaataat gaatgaattt ccttcctata
    21361 gaaaaaaaag cttgagactg cgtagtctca agcttttttt cttttaaaat aaaccaaaca
    21421 gttttactga tttctcttct ggtcaatagt ccacgcttat tctaagaaat cttttaattg
    21481 ttttgaacga gaaggatggc gcaattttct taatgcttta gcctcgatct gacgaatacg
    21541 ttcccgagta acaccaaaca ctttccctac ttcttccaat gttctcgtcc ggccatcatc
    21601 taaaccaaaa cgtaagcgaa ggacattctc ttcccgatca gtcaatgtat ctagtacatc
    21661 ttccaattgt tcttttagta actcataagc tgcgtgttct gctggacttg ttgcttcctg
    21721 gtcttctatg aaatcaccta agtgagagtc atcttcttca ccgattggcg tttcaaggga
    21781 aacaggttct tgcgcgattt tcaagatttc tcggactttt tctgttggaa gatccatttc
    21841 cgcaccgatt tcttctggcg taggttctct tccaaggtct tgcagcaatt gacgttggat
    21901 acgtatcaat ttattaattg tttcaaccat atgaactgga atacggatcg tacgtgcttg
    21961 gtcagcgatt gctcgagtga tggcttgacg aatccaccat gtagcatagg ttgaaaactt
    22021 aaatcctttg cggtagtcaa atttttcaac tgccttcatc agtcccatgt ttccttcttg
    22081 gatcaagtca aggaactgca tgcctcgtcc tacataacgt ttagcgatac ttactactaa
    22141 acgaaggttt gcttctgcca gacgctgttt cgcttcttgg tcgccttctt cgattttcaa
    22201 tgctaacgct acttcttctt ccgcagttaa aagggagaca cggccaattt ctttcagata
    22261 catacgaacc gggtcattga tttttacacc ggttggcgca gaaagatctt ctgtttttgc
    22321 ttgttcagct tttttctcag cactcttcaa gctgtgaatt gatggatcac cattttcatc
    22381 caccacactg atacctgcgt cttcaatctt ttggatcaat ttgtccattt catctgcatt
    22441 caaagtgaat ggtgtagcaa gctgattgga taaatcatca tatacaactt gtcgtttggg
    22501 tttgttttct ttgatgaaag ctgctactgc tgcttcatac gtttttttat ctgttccatt
    22561 tgccataaaa gaaggccccc cttgagtagc tatttggctt gttttaactg tttcgttaaa
    22621 ttgatgattt caatcgtcaa ttcaagctca agctgtttgt ttccagttcg tctggcttct
    22681 tgctgcattt gtttttttaa tgttatttca tcagctaagc cagatttgct gatcacatct
    22741 agcaagtctt ggatttctcg tggcgaactt tcttctgcca ccggtaaata ttcgatttcc
    22801 accaccaacc gccttaaatt ttcatcttgc aaaaagtcta aaaactctgc aaggttgaat
    22861 tcataatgtt gtgagacata tgcatcgaat aaaaaataaa cttctgcata tgcatcatga
    22921 ataaactgaa attctcgttc ttttaaaata ttacggacag aaacttcttt aaatgcacga
    22981 tataatagaa tctgctctgc cttttctact tgcgtcaaag gtctcttttt ccgtgcagtt
    23041 tcttgtctcg taacctgagt gtgttgactt tcctgaggtt cttgacgatt ttgctgattt
    23101 gtccttcgaa gatcgcgcag ttgtttttgt atcgtctctc tgccgatctg aaactctgtt
    23161 gacagttgag atagatacat atcttgttcg attggtgaaa caactcggct aagttcaact
    23221 agtaattctt cgaggtattc gatcttgtct ttttcattat ctaaattttt cccttgtttt
    23281 aaataacgtg atttaaaagt aaagactgtt tcgcgcccat gagccactaa ttcttggaaa
    23341 gatgcttctc catacttacg gagatattca tcaggatcaa gcttttctgg aacgactacg
    23401 acagaaagtg ctaatcgact gttttgccga agcaattcaa tccctcgatt ggttgcttca
    23461 aaacctgcgt tgtctccgtc atagcaaaag accaattctt tcgtcagctt ttccatttgt
    23521 tggatctgtt gagctgttag acttgtcccc atagaagcta atccagtttt gataccagcc
    23581 tgccatgcag caatcacatc cataaaccct tcgaacaaca aaatttctcc gtttttccgg
    23641 atatcgcttc ttgctttatc aagattaaat aaaacttgtc ttttattgaa cagttctgtt
    23701 tctggactat tcaagtattt gggctgatct ttttcctgcc ctttttcagg tgcaagccag
    23761 cgtcctgaaa aaccgatcgt ctggccttga gaattacgaa gtggaaacat cactctttga
    23821 taaaaacgat cagacatacg gccatcatca tgcgtgacaa acaatccaga gcgttctaac
    23881 aattcatttg aaattgattc attcttgaat acttgctgaa gaaaagaacg ctcattaggt
    23941 gcaaaaccta tgttgaattc ttcaattaat tctaaagaca acccacgact ttgcaaatat
    24001 tctaaagctg gctgtccagc ttttgtatgc aatagcatgt gatgatagat ttccgctgct
    24061 ttttcatgag cagcaatcag tttccccgta gcagattcag gattttggac agctatagta
    24121 cgctgacgcc attgatcttc taacggtatc tgttcgaagt ctgccacttt taccactgct
    24181 tctggaaaac ttagaccttc tagttcttga agaaaagtaa acacgtttcc gcctttacca
    24241 caaccaaagc aatgaaaaat ctgcttgtct tctgcaacag aaaaagacgg tgtacgttct
    24301 tcatgaaaag gacacagccc caagtaattt ttaccagact ttttcaactg gacatattgt
    24361 ccaatcacct caacgatatt cgtttgactg cgtactgttt cgataacctc ttgaggaatc
    24421 cgttgagcca ttttttcacc ccctgctcta ttgatatctt ttgggtttaa cagaagatat
    24481 ttcaggagaa taaccggtaa aaaatcgcac taactgaaat cagtgcgatt ttaaaggaca
    24541 caattataca tgtttgattg tatcatattt cattgctttt tcaagtataa cacatttcta
    24601 tctcattaag catcaatttg ctactctttt cagtaactga ttttgaatat cagccttcca
    24661 gtacaatact cgaagaacaa tatttacgag catgaataca aagaagtatc ctacgctctc
    24721 tcccaaccct aagtttactt ccaaaattcc atggaacagc attgcagcta catatatccc
    24781 tacgatcgtc gttactgcta ggaagatgcg caatgggttg aagggataac aagcttttac
    24841 aactgccatg caactgatcc caatcagcaa gtaatacatc aaagtagtag tttcagcatt
    24901 tccccatgcg aatgtttttc caagtagata caccgcaatg atattcacta ctacaagcaa
    24961 ggcatttggt aaagcattaa taagtgctgt aggcaaataa cggtaagtga tttttctttt
    25021 atcttgttca aaagataaaa agaaggaagg atacccttca atcgctaaat caatcagcgt
    25081 gatttggata gggataaacg gaaatccaat tgctgtaacg gcacatataa tcgataaaat
    25141 aaatgaataa atcgttttga taaaaaagat gcctgagact ttcgtcacat tgttgaccac
    25201 tcggcgtcct tcaaacagga cttctggcaa tgtagtaaaa tcagagtcca acaataccag
    25261 attcgcaatc tgacgtgttg ctccatcacc ttctgccata gcaatgctgc aatctgcttc
    25321 tcttaacgcc aaaacatcat tcacaccgtc gcctgtcatt gcaaccgttc tgccgctttc
    25381 tttcaattca tttaccagca atttcttttg ctgcggactc actcgtccaa aaaccgtata
    25441 gcgatgagca gcttctcgca cttccatttc ttcagtaatt tgagaaagat cgacatacgc
    25501 atcataattc ggtaatcctg cacgtctagc gatattggag acagtaaaag gattgtctcc
    25561 agaaatcact ttcagatcca caccttcctc atgaagataa gctagtgttt cttgagcgtt
    25621 ttttcggatt ggatcatcga tttcaaacag agcaaccggc tcaacaaatg ctggtttttc
    25681 ttcgttcaat tgctgatttg gagcgatccc caacataagt acgcgatagc cattcgcttg
    25741 ggctgttttt agactgtccg gaagccgatc ttcttccacc aaacgttcag gcgctcctac
    25801 gtacacgact cccagttctg gaaattccat cgcgccccat ttgcgatcag aagaaaatgc
    25861 gactttgttt gtcacatcat accgattaga aagctcaaaa taatctctca tagcccgcat
    25921 agtgatgttg ttatcactgc tttctgccag atacgtcccc ataatttctg ggatggcttt
    25981 ctcgtacagc ggatgtagtg cttctacccg ctggatactc attttccctt cagtaatggt
    26041 ccctgtttta tccaagcata atgtatcgac atgggccagc gtttcaattg aatacatgtc
    26101 ttgaacaagg attcgttttt ttgccagttt ggttacccct gtcgttaatg caatacttat
    26161 tagcaatacc aaaccctttg gaagcatgcc taacaaagct gctgcagatg caataacaga
    26221 aattttgata gaagcatcgc ggatgaacag tgcttccaaa aatagtatga tccccaaagg
    26281 aataatcacg aaactagtga atttggatac tttgcgaatc gaatctacaa gttcagaacg
    26341 aatagctttg tgagtctttg cctcgctagt caagcggaca gcataattat ctgcacctac
    26401 tcggataact tcagccatga cagaaccgct tgtcaaaaag cttccagaaa gcaattcagc
    26461 tccagtttct ttgacaatca aatcagattc gcctgtcaaa agagcttcat ttgcttctgc
    26521 ccgtccagat ataacctgta aatcggctgg tatctgttct cctgctccga tttttaccaa
    26581 atctcctaat accagctctt tagtagaaat cgattgttct ttaccgtccc ggatcactag
    26641 tacttctgat tgggaaacaa tcgataattt ccgtacaaga tttcttgcat gtatttcttg
    26701 ataaattcca ataacgatat tgacgacaat aattgctaaa tacgccatgt tggaatacgc
    26761 acctacaaat aacagacaaa tcccaatacc aaagttcaga aaattgaata acgtcaatat
    26821 attatcgctg aatatttgtt ttgtactttt tgatgcatct tcctggtagt cattttgctg
    26881 gccagaagcc attttctgaa ctacctcttg ctgtgtaagt ccattcattt tctttttctc
    26941 cattttccgc caactttcaa aaataatcac gtgtttaagt ctagcgtgtt ccaagattaa
    27001 aaacaacttg tttttataaa atttatagaa aaacacacga atgattttac aaaaaagatg
    27061 ccaacctttt tgtttgaatc acccgtgtgc tgtttttatt atcaatctat ctttacttat
    27121 attgttcctc caaacggctc atccaccaac ctagtgctgt accggctata aaaagaacag
    27181 gacaagctag taccccagca actcccaatc ccgcgtaatc ggtaggaatg atcataatcg
    27241 ttgaagcaaa aactacacct aaaatgaaat ggaatagctt gctatatgcc tttgagaaaa
    27301 tgtaatccat gatttttgac aatccaagaa cacaaagaat cccaccaatt ccgatcggca
    27361 caatcacttc aagtcgtact tctttaaatc cgtcagccat cgctttatac aatcccatat
    27421 acaccaaaaa attagaaggg ctcaatcctg ggacgatcat acccaaacca atcaatccgc
    27481 cggcaatcag ccatgttcca aaattctgat cgacttgccc gtcaaataat tgagcgccgt
    27541 ataataaaaa caaataagcc agaacaaaag taacaaccat gatgatcaca tcagaagtat
    27601 ctcttccttt tttccctgct tctttccata acgcaggaac agttccagcg atacatccga
    27661 caaaaaacca taatatcgtt gtttcatacg cacctaataa aaaactgact aaaaaagaaa
    27721 gtagaaagat gcctgtcact ccgcctagac caacaggcag aaaaaacaaa acattttctt
    27781 tgaaattttt tgtaaggtgg gctaaaaaag aaatcagccg ctcatacatg ccaaagacag
    27841 ctgctaatgc accaccgctg actcctggca agataaatcc tgaaccgata aacattccct
    27901 tcacaaaccg taacatccag tctttggttg tgtaccccac ttcttgattt tccatttttt
    27961 gccttttttc cactatttgc gcactccttc tttcatttaa ttttattcat gattgaacat
    28021 ttcctgctat tttacttaaa gactttttca taggtctcta ttattttttc attcattttg
    28081 acaatatcag gataaaacat gttacctcca ttatgataga gatagccgcc attgtaaagc
    28141 aaggactgga tccgccaata tcggtattgt ttcccttcct catttcctaa aagtgggctc
    28201 agcacatctt tagaatacgc ttctgctaat tctactgtgt tttctccgcc atgatttttt
    28261 acataggcaa tataatcttt cccaaaatta tatgcttgga cagctgtcca taaatcgcat
    28321 ccttgtttct ttgcttctac tatcatttcc gccaaatatt tgacaccttg atcaatactg
    28381 tcagctggat tatcaatgat ctcagcttct ccatgcagac tttccgagct ctgcatgaga
    28441 tctgtccctt ttccttttga ttcggtcaat attatcgcct tgatcaaatt ctcgtagttt
    28501 tccatatgat aattttttgc agctgttgtt atttctattt gatagctttc cactcgtttt
    28561 accgaacgat aagttcttcc tcctaaaaac aacaccgcac caataaccgt cagaatgatc
    28621 atgtaaaaaa gaatacggat aatagataat cgttttcgct tcatttcatc acctagttat
    28681 tttgacatct ttttttatct tactatttct ttatcttctt ggctatttct tccttgattt
    28741 ctgtgtacgc cttaagaaaa aatgaatttt cctgtatttt ttgacatctt acgaaaatga
    28801 tcgttgtttt tcagtaaaaa caattgccca atgactgata taatcgttat actttacaca
    28861 gtaggtgatt atgtgaaaaa agaaaagaaa tatgcaaaaa tgccagatgg gagcgaaatt
    28921 tactatgaaa agagcggtca aggcttccct ctctttttac tgcatggcaa tgacggcagc
    28981 ggccgctttt tttcagaaca ggtccctgta ctcgaacgct actataccgt gtatctagta
    29041 gatagccgag gacatggtcg ttcgacaaac gaagcatcta tgttgaattt tcagttaatg
    29101 gccgaggatc tgaatacgat catgttgctg gaaaaaatcg atcaggcaga ttttcttgga
    29161 tttagtgatg gagcaaatct agcactggta tttgccagca gcttccctaa aaaagttcat
    29221 cgattgattt taaattctgg aaacacgctt gttaaaggcg ttcgtttttc tgccagagtc
    29281 atcagtaata ttcattatgc atgggtatgg ttactctctt tatttcgccc aagtcttcgc
    29341 aaaaatcttt tagttatcaa acttttgctg catgacatcg ggcttacaga aaatgattta
    29401 aaaaaaatca actcccccac tttgatcatc gtgggaaaaa aagatgtaat caagctaaaa
    29461 cattctttgt atatagctaa aactatacca aaagcttcct ttgtgcttgt gaaagaacaa
    29521 ggacatgaat tagctagaaa agatcctgaa cgcttcaatc gagaagttct tcaatttctt
    29581 tctgaaacat aactattagg aatagaaggt gatttatttg ttaaaaaata ccatacaatg
    29641 gctgaaaaac cacctaggac tgttcaaaac aatctttttg atttctgtca ttgtcattat
    29701 tgtcggtgaa ttgatgtcca tcggtaaaac tctatctatc cagcaattag gagaaacgtt
    29761 tgccactata cctgtatgga aaagcggact aatgctattg attggtctta tctctgtgtt
    29821 acctatggtt ggttatgata ttattttgaa tcggatgctt gaacaaaagc agaatcctcg
    29881 ttacctgttt gagacaagct ggctgatcaa tacgatcaac aatattgccg gtttcggcgg
    29941 gtttgttagt gtggggcttc gctctgaact atatggacaa aagaaagaga gcagaaacgt
    30001 gatccaagca ttatcgaaag ttttcttgtt tttgatggct ggactttcta tttacagtct
    30061 gctttctttt ttacttgtag tcttcacgcc agttgctcct tttttgaagc aatattggat
    30121 atggttgatc ggcggcggtc tatattttcc tgtcgtcttt ttatttacta ctttcaaaaa
    30181 gaaaggattc cttggtggta cctcacttaa aactcaagga cagctattgc tagtttcatt
    30241 gttggaatgg accggtgtcc tgtttagttt cctttctgtt ggttatttaa tggaaatccg
    30301 gattgatctg tggcaaacaa ttccattatt cattgctgct tcagtgatag gaatcgtgtc
    30361 tatgattcca ggagaaattg ggagtttcga tgtaatgatg attatcgggt tgtccgctat
    30421 tggtgtgcca agagaaatag tcgttgtgtg gatcttgctc tatcgcctgt tctactacat
    30481 cattccgttt ttgattggaa tcgtcttttt cttcaaaaat atcggttcca cttttgatca
    30541 gcgttattct ggtatcccta aacaattagc aacagaaatt gcgcataaaa tcgtggttgt
    30601 cttattatac ttttctggaa tcatgctagt actttctgcc actatcccgc aagcttttac
    30661 agagtttcgc tggcttcata gtttgaaccc attgaaattt cattttatta tccaatttcc
    30721 tagtatacta ttgggatttc ttttaatcat tatgggcaga gggatcgcag cacgtgtgaa
    30781 acgagcatat ttgcctacaa tcttcttgat tgttctagct ttactttatg ttttattaag
    30841 tgattttagt ttcacaccgg tggttttctt gtctattctg cttctgatca ttcttgcttc
    30901 taaaaacgaa ttatttcggg aacaattgat ctattcatgg gagtggcgta cgatcgatgg
    30961 aatcattatt ggtgcactgt ccttgcttta catcgttatt ggggtataca atctgccaga
    31021 tttccctcat cggcgtcacc attttatttc ctttttctta tttccttctg aaaagatttg
    31081 gttttcggga ttgttggcca tcatcgctgt tagtttcatg atcgttctat ttgttcattt
    31141 ccttcaagga gaaaagaaac agattggcga agcctttaat gaagaaaagg ccctgaagat
    31201 ccttacaact tatggaggaa actccgatag tcaattgatc ttcttaaaag ataagcggat
    31261 gtttgcctat gaaaaagacg gtgaaccaac tgttcttctc caattcgctt gcttcaataa
    31321 taaatgtatt gtaatgggtg atccttctgg taagaaagaa gattttccag aagcaatcga
    31381 agcttttatc gaagaaacag atcgactatg ctatttacca gttttctacg aaacaagcga
    31441 agagattgtt atgattttgc atgaattcgg ctacgatttc attaagatgg gggaagaagc
    31501 ttatgtagac ctgaatagtt ttacaacttc agggaaaaaa atgaagggca ctcgtgctgt
    31561 cttgaatcgg atcgaacgag aaggatttac atttgatgtc ttacaaccac cattttctac
    31621 tgagcaaatg agtactttca aaaatatctc tgataattgg ttgggttcac gaaaggaaaa
    31681 agggttttct ttaggctttt tctctgaaga ttatctacaa agagcgccta ttgcagttgt
    31741 caaaaatacc caagaagaaa ttgtcgcttt tgcaacgatc atgcctactt acacgaacaa
    31801 taaagtagga acgattgatc tgatgcgata tgattctaaa aaagcacctt ctggcagtat
    31861 ggatttcttg ttcttgcatt tgtttgccta tatgaaagaa gaacatatcc aatggttcaa
    31921 tttagggatg gcccctcttt caaatgtcgg aacttccaga aagagttttt tacaggaaag
    31981 gattgcatac ttggtttacg agtttggttc tcatttttat tctttccatg gattaagaga
    32041 atataaaagc aagtacgcta caaactgggt ctcacgttat actctatatt ctagagatag
    32101 ttggattgca tatgttatga ttgctttgct gattattgat aatgcgccag ttgagcagac
    32161 acaaggtaga ataagtggtt tgaaaaaatg gatacgaaga aagtattaaa aactgcatac
    32221 cgatagatga acactctcat gatgaataaa agagactgaa gtaaaacgaa agttcatgct
    32281 tctacaatct cttcttatac aattgaagaa caaagagcgg tccaaacaga aatcctgttt
    32341 ggaccgctct tttattttaa acatttttca ttaccaaaat attcgagtcg cgtttcttat
    32401 gcttcgatct ctgtaacgat acctgaacct actgtccgtc cgccttcacg aattgagaat
    32461 gtcgttccgt tttcaacagc gattggatgg atcaagtcaa catcaatcgt cacgttgtca
    32521 ccaggcatga ccatttctgt atcttctggc aatgtgattg ttcctgtaac atcagttgtg
    32581 cggaagtaga actgtggacg atagttgttg aagaatggag tatgacgtcc accttcttct
    32641 tttgtcaaca cgtacacttc tgctttgaat tttgtatgtg gagtaatgga acctggttta
    32701 gcaagtactt gtccacgttc gatatcgtca cgttggatcc cccgtaacaa cacgcctacg
    32761 ttatccccag cttccccgta atctaacgtt ttacggaaca tttctacacc tgttacaact
    32821 gctttttgtg tttcaggttt gatccctacg atctctacct catcaccgac acgaacagca
    32881 ccacgatcaa tacgacctga ggcaacagtt cctcgacctg taattgagaa gacgtcttcc
    32941 actggcaata gtaatggttt atctgtatca cgttctggtg ttgggatata ttcatctact
    33001 gtatccatca attccataat agcagcttca gcatctggat cgccttgcaa tgcttttaat
    33061 gcggaacctt tgatcacagg agtgtcatcg cctgggaaac catattcgct caataactcg
    33121 cgaacttcca tttctaccaa atcgatcaat tcttcatcat cgactaaatc aactttgttc
    33181 aagaaaacaa tcaggtattt tacaccaact tggcgtgaca ataaaatgtg ttcacgtgtt
    33241 tgaggcatcg gaccgtcagt tgcagaaaca actaagattg caccatccat ttgtgctgcc
    33301 cctgtaatca tgtttttcac atagtccgcg tgtcctggtg catcaatatg cgcgtagtgg
    33361 cgtttttctg tttcatattc tacgtgtgcg gtattgatcg taatcccacg ttcacgttct
    33421 tctggagctg catcaatact agcatagtct tgaggatttg ctaggccttt tttgcctaat
    33481 actgtagtga tcgcagcagt taaagtagtt ttcccatggt caacgtgtcc aatcgtacca
    33541 atgttaacgt gtggtttact tctgtcataa tgttcttttg ccataattta caaacctcct
    33601 aaataatata gatcactgaa aaatttcagc tctcaccttt ttattatagt ctttttttga
    33661 ctaaatcaaa aagaatacgt ccgacttact ttttgtaaga ttcgaactaa gcttattata
    33721 cgtcaatatt ggtcaaagtc aactaagagc actcgtctgt cttatacgcg aaaaaagtac
    33781 cttttataaa taaaaaaatg tgttttttga gaaaaacttc aagatagtcc ttaactttct
    33841 tttgaagcaa taagaacaaa atgaggaaaa gtttaaaatt cagctgttgc caacaacttt
    33901 caatagacga aatcttctat ttcgttaata ataaggaaag aggtgaagta catgaaaaaa
    33961 ttaattggcg tacttgtcgt tttaggcata tttctcggcg gaagttttgc agcttatcat
    34021 tatttttacg gcggcgaagc ttattataca aaaatcacga cgaatggcga aatgtcagct
    34081 acccaagcag ataacggaga gaaattcacg acttatacct acaagcaagc ggcttataat
    34141 aaagatggtg aaaaaaaaca ggtgacgctt cgcgaagaaa gagaacatcc attaaagatg
    34201 aatgcgtatc ttaaactcaa agtcaatcct cgtaaaggtg tattaagctg ggaagaaata
    34261 aaggcttctg aagtgcctaa aaatgcggca gaaaaaatcg ataattagta aatggtctta
    34321 aaattttatt agataaaaag aggctgggac agaagcgttt aactccaaga aata
//
LOCUS       DACGLW010000022        33972 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.022, whole genome shotgun sequence.
ACCESSION   DACGLW010000022 DACGLW010000000
VERSION     DACGLW010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33972)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33972)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33972
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.022"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            23..646
                     /locus_tag="IXT53_001101"
     CDS             23..646
                     /locus_tag="IXT53_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4904945.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1096..2145
                     /locus_tag="IXT53_001102"
     CDS             1096..2145
                     /locus_tag="IXT53_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ4904946.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2142..3023
                     /locus_tag="IXT53_001103"
     CDS             2142..3023
                     /locus_tag="IXT53_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ4904947.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3026..3796
                     /locus_tag="IXT53_001104"
     CDS             3026..3796
                     /locus_tag="IXT53_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ4904948.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4075..4929
                     /gene="aroE"
                     /locus_tag="IXT53_001105"
     CDS             4075..4929
                     /gene="aroE"
                     /locus_tag="IXT53_001105"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HAQ4904949.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5107..6270
                     /locus_tag="IXT53_001106"
     CDS             5107..6270
                     /locus_tag="IXT53_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAQ4904950.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6387..6797
                     /locus_tag="IXT53_001107"
     CDS             6387..6797
                     /locus_tag="IXT53_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HAQ4904951.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6971..9199
                     /gene="topB"
                     /locus_tag="IXT53_001108"
     CDS             6971..9199
                     /gene="topB"
                     /locus_tag="IXT53_001108"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ4904952.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9337..9654
                     /locus_tag="IXT53_001109"
     CDS             9337..9654
                     /locus_tag="IXT53_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4904953.1"
                     /translation="MKKVHSFLNYLMGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAY
                     SPTLILSTLTAGAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQ
                     YFG"
     gene            9814..10050
                     /locus_tag="IXT53_001110"
     CDS             9814..10050
                     /locus_tag="IXT53_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ4904954.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10047..10445
                     /locus_tag="IXT53_001111"
     CDS             10047..10445
                     /locus_tag="IXT53_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAQ4904955.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            11524..12913
                     /gene="ltrA"
                     /locus_tag="IXT53_001112"
                     /pseudo
     CDS             11524..12913
                     /gene="ltrA"
                     /locus_tag="IXT53_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
     gene            complement(13012..13956)
                     /locus_tag="IXT53_001113"
     CDS             complement(13012..13956)
                     /locus_tag="IXT53_001113"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HAQ4904956.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(14252..15196)
                     /locus_tag="IXT53_001114"
     CDS             complement(14252..15196)
                     /locus_tag="IXT53_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ4904957.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            15417..16631
                     /locus_tag="IXT53_001115"
     CDS             15417..16631
                     /locus_tag="IXT53_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ4904958.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            16653..18668
                     /locus_tag="IXT53_001116"
                     /pseudo
     CDS             16653..18668
                     /locus_tag="IXT53_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-rhamnosidase"
     gene            complement(18751..20124)
                     /locus_tag="IXT53_001117"
     CDS             complement(18751..20124)
                     /locus_tag="IXT53_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAQ4904959.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(20150..20434)
                     /locus_tag="IXT53_001118"
     CDS             complement(20150..20434)
                     /locus_tag="IXT53_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ4904960.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(20436..22451)
                     /locus_tag="IXT53_001119"
     CDS             complement(20436..22451)
                     /locus_tag="IXT53_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ4904961.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(22630..22935)
                     /locus_tag="IXT53_001120"
     CDS             complement(22630..22935)
                     /locus_tag="IXT53_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HAQ4904962.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(23024..24055)
                     /locus_tag="IXT53_001121"
     CDS             complement(23024..24055)
                     /locus_tag="IXT53_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HAQ4904963.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            24262..25137
                     /locus_tag="IXT53_001122"
     CDS             24262..25137
                     /locus_tag="IXT53_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ4904964.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(25289..26131)
                     /locus_tag="IXT53_001123"
     CDS             complement(25289..26131)
                     /locus_tag="IXT53_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ4904965.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(26223..28100)
                     /locus_tag="IXT53_001124"
     CDS             complement(26223..28100)
                     /locus_tag="IXT53_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ4904966.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(28097..29518)
                     /locus_tag="IXT53_001125"
     CDS             complement(28097..29518)
                     /locus_tag="IXT53_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HAQ4904967.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(29521..32911)
                     /locus_tag="IXT53_001126"
                     /pseudo
     CDS             complement(29521..32911)
                     /locus_tag="IXT53_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
     gene            33168..>33972
                     /locus_tag="IXT53_001127"
     CDS             33168..>33972
                     /locus_tag="IXT53_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ4904968.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEI"
BASE COUNT        10557 a         6151 c         6626 g        10638 t
ORIGIN      
        1 aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat cgagagtaca
       61 agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt attttcgacg
      121 tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt tattgccgga
      181 aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa ccagcaaatt
      241 gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg cgatatcggt
      301 gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt ttaatgatcc
      361 tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt atgatgtaca
      421 tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt tctttgatgg
      481 aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt ttgataagga
      541 gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag aagaagaatt
      601 ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca ttggcgcgat
      661 gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg tttgatcttc
      721 aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt cgattagact
      781 taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt atttcagcag
      841 aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg agactttccc
      901 atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga catgaacatt
      961 gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga ccgctttggc
     1021 cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa agattgactt
     1081 tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat tattttagtt
     1141 tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag tacttcgacg
     1201 aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca tccggcatta
     1261 gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa agaaggagaa
     1321 aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc cacaatgagt
     1381 caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc tgccgctcaa
     1441 tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac ggatcctgtg
     1501 ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg tgtttctgat
     1561 cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa tgcaaaaaaa
     1621 gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc acaagcagaa
     1681 gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc cactaatgag
     1741 attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt tccattagat
     1801 aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc aaatatcccc
     1861 gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat cgggatcaat
     1921 caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag tggaaaagaa
     1981 aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat caatcaatct
     2041 caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc caaaatcatt
     2101 acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta tcagctattt
     2161 cacaaggcat gctttgggca atattaggat tagggatttt catgacttat cggatcttag
     2221 actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc tgtgtgacag
     2281 ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt gctggcatga
     2341 tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt attttagcag
     2401 ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga acaccaaatc
     2461 tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg ttacctgatt
     2521 actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct gtactgctat
     2581 tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac gaaacgatgg
     2641 ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact ttgtcgaacg
     2701 gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca gatgtaaata
     2761 aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa gtgatcttcc
     2821 aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc atttatcagc
     2881 tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag ttgttctccg
     2941 ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg aacttaaaaa
     3001 cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa atggcagaaa
     3061 agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta accttacaat
     3121 cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa gcacactgtt
     3181 caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta aagatcagct
     3241 gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt tccaagaccc
     3301 caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg cacaaaaaag
     3361 aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag attttttcag
     3421 actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag cgggtgaatt
     3481 atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag ctcctgagtt
     3541 actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag cattaatgcg
     3601 attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca cgcatcgaat
     3661 ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga aaatcgttca
     3721 agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc agttctttga
     3781 agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact tgaaaactca
     3841 ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa catatttggg
     3901 tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt ctttgctctg
     3961 acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc gtactttttc
     4021 gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa taagatgatt
     4081 tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag tttatcccca
     4141 ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt agctttccaa
     4201 gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga tatgttaggt
     4261 gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga ggtaagccag
     4321 gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg tttgattggc
     4381 tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat ttcgatcaaa
     4441 aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac tgttcaagct
     4501 gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac attcgctcaa
     4561 gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa agttcgtgac
     4621 tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct agtcaatgca
     4681 acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat aagcatgatg
     4741 catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg tttattaaaa
     4801 gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat ctaccaagga
     4861 gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat tgaaaagctg
     4921 tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg gtctcaagaa
     4981 aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat tatcaaaaaa
     5041 tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa acgaggagga
     5101 accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa acctctgaag
     5161 cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat ggaaaatgag
     5221 gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt gaaccaatac
     5281 gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa taaaaaattt
     5341 ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc gtggaaaaaa
     5401 atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt ttttattggt
     5461 aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac ctttcatggg
     5521 caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac acgcggagaa
     5581 gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc ccataagccg
     5641 aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg acaaaataag
     5701 atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt agtagaaaat
     5761 tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga aaaagtgatc
     5821 gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa tctggatggt
     5881 gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca gtacgcacgt
     5941 ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca agtaatgaac
     6001 gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta tgcagctggt
     6061 aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa aaacctcttt
     6121 tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga cggttcgtgg
     6181 aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca aatgaatcaa
     6241 tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag aagctgcgca
     6301 aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc ttatggtaga
     6361 tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa aatgagtcgc
     6421 gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt atcaccacat
     6481 acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa aatctaatca
     6541 cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg ccagaaggaa
     6601 ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca agaaatgaag
     6661 aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag atgccactgg
     6721 atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc atcaactggc
     6781 agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa gcagcaaacg
     6841 gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt atcttttttt
     6901 tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat taagtaacgg
     6961 aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa aagcctatgc
     7021 cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg atccattgtt
     7081 tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg ttgctccagg
     7141 agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt tcccagaaac
     7201 cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa aacgagaatt
     7261 gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg aagccatcgc
     7321 gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt tacgtttatg
     7381 gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc aacctggaaa
     7441 gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt ggctgattgg
     7501 aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg atggcagttt
     7561 ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc aagaagaaat
     7621 caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga caaacaaagg
     7681 gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag agttaacgaa
     7741 ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc ttgaagttac
     7801 gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt tgcagacaaa
     7861 gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc aaggattgta
     7921 cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag aaaatgagca
     7981 tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag aagaagtaca
     8041 gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc aagaacatca
     8101 tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc tttccaaatt
     8161 acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg ctgcagatta
     8221 tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa aaagcatcgg
     8281 aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa aaaagaaaga
     8341 agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac tgaaaagtat
     8401 ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca cagcaatgaa
     8461 aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag aagtagaagg
     8521 aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga agcattacat
     8581 cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt gtaaggcagt
     8641 agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag ggtacttgag
     8701 aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca aaaaattcat
     8761 cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt cagatgagat
     8821 tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag gaagatgtcc
     8881 aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct tgaatccgga
     8941 atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa cgaatctcag
     9001 agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg gtaaaaaagg
     9061 gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt cttttccatg
     9121 gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa gaaaaaataa
     9181 tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt ccgaaaagtg
     9241 agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa cgttggggta
     9301 ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca ttcattttta
     9361 aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct aaagtatcct
     9421 gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc accaacgttg
     9481 attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca taaaacgaca
     9541 agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc ggctatgatc
     9601 attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg ttaagaatac
     9661 gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtcattttt tcgtaagaaa
     9721 aagcaaaata aaagtatata cattgattaa aagtatatac ttggatataa tgtacttact
     9781 atagaatgag aatggtatag agggtggcga aatatgaaaa aaatgaaaaa gtataaaatt
     9841 agaaaatctg gcaatagcga tgtaacaact atcccaccag aagtaaaaga attcatgggt
     9901 gtgcagacag gtgacgcaat cagttatgtt tttcaatctg atggttctgt acgaatgatc
     9961 aaagcacaag aagaacctga tattgattca ttagttgatt cgatcatgaa tcaatatgaa
    10021 gacgcgttaa aagatttggt tgatttatga ggtatctttc gactaaagaa attatcaaaa
    10081 tcaacgcgaa agttatttta cgtcactcgc caggcgaaat gatcggtgta aaagatgcaa
    10141 atgcattaga tatggccgtc aaacagcctt cacaagcagt attcaatcaa gaactctatc
    10201 ctgaaatcta tgaaaaagcg gcgattttag caattaattt agctaaaaaa catccttttc
    10261 ataatggcaa taaacgaact gctcttgttg ccatgttgtt gtttcttcaa ttgaataatt
    10321 gtccagttgc tttttctcgt caagaagctg ttgattttat cataatgatt accactagtt
    10381 cattggattt cgattcacta aagtccaaga taaccagtta tttacgacaa accgcaataa
    10441 aatgaacgaa ttaaaaactt tatttctcaa ttagatcagc atgtcattaa ggaatatgct
    10501 gattttttta tgtttaatca tacaaaatag ttttttcata aaaatatgga aaatatggat
    10561 taattttgaa ataattgatt taaaaggaat acaaaagagt gaatagtagg aaatagtggt
    10621 ttagaactag tgggctgatt ttttatatat tttagtttac ataatatata ttatacaaag
    10681 tagatttaaa aaatgataaa cctctctcga acggtttttt atcaaaaatc gtcatttttc
    10741 catcaaattc tgcttatgca aaattataaa aaaattttgc agtttttctt actttttttg
    10801 tcgtttaatt gaatgataaa aaccagtttc tttattcagt taatcaaata tgaacgaatt
    10861 agatatgaat cattgtattt gccgttgcgg aaggattttt taattaacca cagataaaca
    10921 ttgataacga ttcgcttatt gtaatcaaca gattaaattt catatgttca tatgtacgga
    10981 tacattttcg gaccgattaa ttttaaccat tctatttatt tgcttaccta ttagatatca
    11041 ataaaaaact gcttaacagc tatttcagct gttaagcagt tttaggatgt gcgccctgca
    11101 tgggtgaaaa cttggtggtg aaagtccact acaggcttgg cagtaggaac tgttagcgaa
    11161 agacaaggcg gcaatcgtga ggttgtggct gaaggaagtc ggacgcaaac tcctgaactg
    11221 acgaacagaa actagataca aggctgaatt aggtcggata agtcttcatc acaagacaaa
    11281 gtccaatact gcccgaaacc tatacagtaa atctagcagt tacatgggag gaaagttttc
    11341 actcttaccg ggggagatct catgagggtg ttgtgggcgt tgaatgaaaa caaaccagca
    11401 caacgatcat ggcgacatga tggtgaatca tgagaagtca gccgaggtca tagtagtaac
    11461 gacactgacc gttacgaagg actgaacaat aagaatcttg aataatttag gaggtgtgag
    11521 aagatgcgac gaccgcagaa aacaagaaaa cttggctatc atcaaagagc tagtgtggaa
    11581 cacgaagggt atgatgaagt gcgcagcact tcttgcggtg aacagaggaa acaagatggt
    11641 gtagaaaatc tgtttgggag aattctagat cggaataatc tcaatcaagc gtatcttcaa
    11701 gtagtcagaa ataaaggggc ggctggtgca gatgggatga ccgtcgatca actgcttcct
    11761 tacttgaaag agcaccgcga ggaattacta tccaatttac gccaagggaa ctataaacct
    11821 cagcctgttt tgcgagtaga aataccgaaa ccaaacggcg gtgtaagaaa actaggaatt
    11881 cccacggtta ttgatcgtat gcttcaacag gcaatcaatc aagtgttaca accgctattt
    11941 gaaccagaat tctcagataa tagttatggg tttcgcccaa atcgcagcgc acatgacgca
    12001 gtgaagaaag caaaatctta ttatgaacaa ggttaccgat atgtggtgga cattgatatg
    12061 aaagcctact ttgacaccgt aaatcacgat aagctgatgt atttcattga gcggaaagta
    12121 aaggacaaac aagtcctaaa actgatccgt caatacttaa tgagtggtgt aatggaaaat
    12181 ggactcatca caacaactga agaaggaacg ccccaaggag gcaatctctc tcccctgtta
    12241 agtaacatct acctgaacga atttgatcaa gtgctagaaa atctaggaca taaatttgtt
    12301 cgatacgccg atgactgcaa tatctatgtt agaagcaaac gagcaggaca gcgtgtgatg
    12361 aagaaatcca ttcaatttct cgaagaacaa ctaaagttga cggtaaatcg agaaaagagt
    12421 gcggtaggaa gtccattgaa acgaaagttt cttggattct gcattctgcc aacaaagaaa
    12481 ggtattaaaa ttcgccctca cgcaaaagca aaacagcgtg tgaaaagcaa gctcaaacaa
    12541 ctcactaaaa gaaaccgtgg aagaagtatc caacttattt taaaagaaat caagcaacta
    12601 atgactggtt ggatcaacta ctatggaatt ggtgagatga aagaattcat gagggagtta
    12661 aacggttggt tgaagcgaag aatcagacaa tatatttgga aacaatggaa gaatccaaga
    12721 accaaaagaa aaaaatctga ttcgtttagg gatcgataaa gcgaaagcgt atgaatggtc
    12781 aaatacaaga aaaggtattt ggtcagtctc caaaagtcat attcttcatc gatcattaac
    12841 agacaaagaa ctggcccgcc aaggatatga ggacatctcc ttgaagtacc agttcgtaca
    12901 ctcaacttat tgaaccgccg tatacgggtc cgtacgtacg gtggtgtgag aggggcggaa
    12961 ggtcagttgg ctttcccctc tactcgattt ttagttcata ttcattgttt cttataatac
    13021 agtgttcaat acttctctta aagcacttgt tgatttgctg aacattgctt tttcttcttc
    13081 gttgattgat aattcaacaa cttcacgaac accattttcg tcaacgattg atggcacacc
    13141 tgtaaagata tctttttcgt cgtattcacc agttaaataa gcagatacag gcaatacagc
    13201 ttgttcgtta ttcaagatag cttttacgat acgtgcagta ctcataccaa taccatagta
    13261 agttgctttt ttacgatcaa tgatctcata agcagcatta cggactttat cagcaatcac
    13321 atctaattca tcttgggcaa tacgatggtc tttttctaca atttcaaaca ctggtttacc
    13381 accgacagtt gtatgtgacc atgcagcaac ttctgaatca ccatgttcac ctaagatata
    13441 gccgtggacg ctgcgtgggt caacttttaa tttcaatgcg atttctttac ggaaacgagt
    13501 tgtatccaat gttgttccag taccgataac acgtgatgtt ggcagaccag attcgttcca
    13561 agcgatatat gtcaaaatat ctactgggtt tgaagcaaca acgataatac cgtcgaatcc
    13621 tgatcccatg atttctttta cgatttggcg catgattgac gcatttgttt taactaaatc
    13681 taaacgagat tgaccaggtt tttggttgat accagctgtg ataacaacga tatcagcatc
    13741 tttacattct tcatagccac ctgaccatac agccacattt tcatcgcccc aagccatacc
    13801 atctaaaagg tccaatgctt ccccttctgc tttttcttga ttcacatcga tcaatactaa
    13861 ttcattagag attccttggt tgatcattgc ataagcgata cttgttccaa caaaacctgt
    13921 accgacgatt actactttac gacttgtttt tttcatgaat aaacagctcc ttttcataac
    13981 tatttgttac tttcatgaaa agtatagtct tgtttattca ttttatcaac atgttaatta
    14041 taatagttgt tattataatt tttgatggaa acgaattcct gaatacgcat aacagataaa
    14101 tgacaaatgt ttacacctat atcccctgat aatacaacca aaagtttaga actgaaaata
    14161 aatccaaaaa aattcgctgc aacgatataa taagacagca atcgcaccta aaacaatttt
    14221 ccttcatatg ctttagtgaa agattttctt cttaatgttt atttctccat tcattgggag
    14281 tacatgttga atacttcttg aataatttga ataaagaagt cgtttgttta tatccgatac
    14341 gattagcgat ttcttccaac gaaaaactcg tttcttgaat cagttccaac gcaaagtcga
    14401 attttttctt tagaaccagt tcttgaaagg aagcatcaaa atacattttc aatttattac
    14461 taagatagac cggattgtag ccaaaatgag ctgctgtttc ttttaacgta caagaagtaa
    14521 aatgcttatc gatataatct aatactctaa gcatctcatt ttctgcgcat ccatctgctg
    14581 atgaaaagct ttgttgttct aataaatgag tcaattcgat cagtaatagt gatgtataga
    14641 gatttaacga acggatctta tgactgtatg tatcaaaata aatcaacagc atattttcaa
    14701 tcaaatgcgt caaacgactt gtttttccgc cgcgaaaatg aatgaattgt gtatgttccc
    14761 ctttttcttt tgatgcattt atcaaaaaag aagaaagtag attatttgtg cttgccaaat
    14821 gattcaatat ctctgtagat aaggtttctt cttttaataa gatgttgatt aaaatatcct
    14881 cttgccctgt gtattcgatt ctttgagtag cttctttatc aatgatcacc acgtcacttt
    14941 cttccatctc tatcctttct ccattaataa tttgtaaaca tgtaccgcta tacatataat
    15001 tgatttcaac aaaatcatgt gtatgctcgg gaacttccgc atatcgacgg tgtttactga
    15061 tatgaatatt tccctgttga aatagatccc ctcggtggat ttttggcggt gtctgaagat
    15121 ggtaagctaa gtttaaatca ttgatattca ttccttgttc acgctgcaga gtttcgattg
    15181 attcttcgtt acgcaaaaaa aatgtctaat tcagctagtt ccaattgact ccctcctttt
    15241 tgtaagcggt ttctttcgct tattagttca cgctgtactg ctattatatg caaagctatt
    15301 tttggtttca taaagacttt ctatggttgg gacatgaact tcctgtgatt ccgctattga
    15361 gagaataacc acttcatact atatttttga taagcgctta caaaaatgaa aggaacatgc
    15421 ttatgaataa aaagatgatg aaattttctc tgctgtcagt ctctcttatt ttgacttctg
    15481 ctggtgcagt cacgtctgca attcccagta tgtcagctac ttatgccacg cgttctttat
    15541 ctgctgttga attattaacg actgctccct cttttatggt cactattttt gttttgatta
    15601 gtagttttat tgctaggaaa atcggacaaa aacaaactgt gatcatcgga ttagtgattg
    15661 catcgatttg tggtatcctc cccctcttct cacaaagtta cagtattgtc ttagcttcaa
    15721 gagctggact aggtattggc tttggattga tcaattcttt ggcagtcagc atgattgctg
    15781 ccttttatca cggagatgaa cgagcctcta tgattggttt tcaaagtgca tttcaaggat
    15841 ttggtgcagc tttaatgaca tttgttgcag gacagctagt acagttcggt tggcaatata
    15901 cctttttagt ctacgccatc agcttaccta ttcttgtctt gtttgttttg tttgttccta
    15961 cacctgaaac aactgaactt caatcgcatg gcacagcccc aacaagacaa gtaatcaaaa
    16021 cgaatcctaa aatgattagt tatggtattt ttctcttgct ggtcatcgtg gtatataatg
    16081 ctgtatcgat taaattagcc acagtcctga cgacttattc tctaggaaac gaaactaatg
    16141 cagcgaatat cttaacaatc atgcaattgg caagtatggc tgccggagtt tgttttggct
    16201 ggcttcagac aaaagcacat cgctatatgc ttacactttc tttactactg atgggaatcg
    16261 gctttgtatt gattgccact gtgataaacc tgtatgtgat tgggtttggt gcgatcctaa
    16321 caggaatttc tttttcgtta ttcattcctt acttattcaa ccaagtaagt atccaagcgc
    16381 ccaagtacgc ggagagtttc aatacttcaa tcttattggt tggcgctaat ttaggtactt
    16441 tcattgcacc atatggtttg ctctttctct cttttgcttc tcttgggagt aaaacacttg
    16501 ctgttttcgt caatggcagt atcttgctgt tgattagcgc gcttattagc cttctagtag
    16561 tacacaggac gaatagaaag acagtgataa gagtagatga agaaataaaa gaacaattga
    16621 caacaaaata aatttgatgg aggtcgtgta aaatgacaaa tgaaaaacat aaagatagat
    16681 ggctttggta tcctggagac tttgagatcc gccatggact gctacaaaat ttccaacggg
    16741 aagaacgagg ttttgactgg ccagcgaact ggtacatgga tgattgccat cggaacgtca
    16801 agtttaagag atattacttt cttgatcaac cttccatgtt taaagtgacg atccaaggag
    16861 ttgggtatgt agaaattaat ggacaaaaac atccatgtgg aaaatggctg acttgtccag
    16921 ctggtaaagc aaaaattcgt atctttgtag gacatacatc agggctacca gcgatgttta
    16981 tcgagggaga cgaagtaaaa tcagatattg gctggacagc aagtaatttt attgaagaat
    17041 atccagctgg ctggagtccg ctttatgtcg atatcgcaaa agatccaaat caaatctatt
    17101 atcaaaaaga atgcgtccat ccagtcactg aaacggagat tggcggagga atcttattcg
    17161 attttgggcg agcagtgaat ggctgtgtag aactaatatc tctggatgac catatcgaat
    17221 ctatcactct atgttatgga gagtccgatg tagaagctat ggatatcgat tattgctact
    17281 ataagcaaat aggaatgaca agaaaaatga aagctcgtaa acgcgctttt cgctatttgt
    17341 ttataccaga ggtttctact ggaatggttc aacttcaagt ttttcatgaa tttttatctc
    17401 tttcgaaaca agctgctttt tcaagcgatt cttctttact gaatcagatc tggtcagtat
    17461 cagaagaagc cttcaagtta tgtagtgatt tgttttttat cgacggtatc aaacgagatc
    17521 gctggatttg gtctggagat gcgtatcaat cattcatgat caatcaatat cttttttttg
    17581 acgaggaaat caataaacga acgatattag cattaagagg acaaaatgaa atcaagcagc
    17641 atatgaatac tatcgttgac tattcgatct tgtggttgat tggtatcgaa aaccactata
    17701 tgatgacgaa agactatgaa tttttacgca tcgtctaccc taagatgcaa tcattaatga
    17761 gataattgat ggagcaaacg aatgaattag ggtttattta tgggagagca aaagattgga
    17821 tatttattga ttggtctgaa atggataaag aaggaacgct tgctgcagaa caagtcttat
    17881 tactgaaagc ctatcattcc atgatcactt gcggagatat cttagctgaa acatatggag
    17941 agaacgaaaa ttatcatact catctctatc aagaaaaata tcaactattg aaacaaaacc
    18001 tatttgacta cttttgggat gaagaacttg gcgcttttat cgattgctat gaatctggga
    18061 gaagaaatgt gacgcgtcat gcgaatattt tcgctgtttt attcgatttt actaacaaag
    18121 accaacagca atcgatctta aagaatgtct tattgaatga cactatcaca cagattacta
    18181 ctccatattt caaattctat gaacaagatg ctttatgcaa actcggacag acgaatatcg
    18241 tctaccaaac gattcttgat tattggggag ggatgttatc tcatgatgcg gtaactttct
    18301 gggaagaata cgaccctact caaacaggaa atgaaaagta cagtatgtat ggagatcctt
    18361 atggaaaaag cttatgccat gcatggggag caagcccaat ctatctttta ggacgctatt
    18421 ttatcggttt gtatccaaca aagccaggtt atgaagaatt tatgatacaa cctaatctag
    18481 attcatttga aacactgaat tgtacgctac ctatcaaaga tggaaagatt tcgcttgaaa
    18541 aaacaaaaga taagatcgtg attacttctg atagaacagg cggaacagtg aaaatgaatg
    18601 gtaaaagtta tcctcttcta gcaaaacagc cacttgtctt aacaagctat tcaaaaataa
    18661 atgcataaaa caaaaaactg cttgcgacat atcagaagac tttaaatctt cttctgatat
    18721 tgtccaagca atttttattt tgaagtttgc ttaattctgt tctttagaaa caatgtttgt
    18781 tctttttccg gcaaaaagaa agctagataa tacgacaagt aaaaagaaag cgatgattcc
    18841 aactgtcaag gctatttgac cgtattctcc tagtttccca agtacaagtc ctggtactag
    18901 ataatcagta tcggcaaatg tagaagaagc gattttaaga tctcctaata ctggaaggat
    18961 aaataatggc agccaactaa tcaataagct attgaaagca gaacctaata ccgcccctct
    19021 tctaccgcca gtcgagttgc cgaagacacc agaagcagct ccgcagaaga aatgtcctac
    19081 taccccagga atgataactg tagtttgcaa gcctatcata gcaaacatgg atagtgttcc
    19141 tacaaagaag ctaacaaaaa agccaattaa tacagcatta ggtgcatatg gaaaaacgat
    19201 aggaacatct aatgctggtt tagaatcggg tacgagtttt ttagcaatcc cttggaatgc
    19261 tggaacgatt tctgccaaca ccattcgaac cccttgaagg acgataacaa acccagctgt
    19321 gaatgttcca gcttggatca gtgcaaagat tacagcgttg tctccattgc ttagttctct
    19381 ttcaacaaat ccaggaccag ctaacaaagc aagtgcgaca tatacgatcg acatcaagag
    19441 catgatcgat acagtagaat ctcgcaaaaa gctgaaactt ttaggaatct ttatgtcctc
    19501 tgtagaacgg tctttgtttc caaaaaactt tccgatccat ccactagaag cataaccgat
    19561 atttccggta tgccctaacg caacttgatt gtttccagtg atgttcttca taaacggttg
    19621 acaaagagca ggcgtaatag ttaatagtgt tccttcgaag accccgccta ataagatcat
    19681 ccacaaattt gcagtaaacc ccgcgcttac taaaatgact gctgttagac aagaaacata
    19741 aagcattgct tgacctgtaa gaaaaatgaa tttatatctt gtgaatctag caagtaaaat
    19801 attcacgatc atccctacta gcatgatcaa agcagtaggt gtaccgtatt gttgcaaagc
    19861 aatcgccact actgcttcat tgctaggaac tactccttga gtatgcagag ctgtttggaa
    19921 catcgaagca aaaggggtta gtgaattact gattaaacca gcaccgccga tcagtactag
    19981 gaatccgaca aatgttttga tagttccttg gatcacttct gaaaatttct tcttctgaag
    20041 aacgagacca agcagtgcaa tcaaagaaac gaggatggag ggctgactga cgattgacac
    20101 aaacaaattc aacaatttat ccattgatag ttcctccctt gaatcatttt tattcttttg
    20161 acaagatctt cgttactttt tcttttactt cttcgctgtc aatgatcgat tgcaaaggta
    20221 cgagcttttc atcaggtata gaagttagcg tatctttcaa agaatgctca atgaagaaat
    20281 aatcagccat atcgctagat acacttccaa catccgtatg acttacttca aactcgtcca
    20341 catctgctcc aagatctgca agtgcttgtt tgatgttcat ctctaccata aaacttgttc
    20401 ctaatccact ttgacatact gcaaaaattt tcatatcatt cgtctccttt tcttatgatc
    20461 gctaatattt cttcttttgt tgatgcgtgt aataaatctt gaagattttg tttattagaa
    20521 agtagctttg ttaaatttgc caaagcccga agatgtgcct cattatcgat agcagctaaa
    20581 cagatgaaca aactaatggg atgggctgga ttatcaagca gatccacagg ttgttgaagt
    20641 ttcagcaaag acatccctac ttcgtttacg ccatcttctg gacgagcatg aggtaatgcg
    20701 atgtgatcgc caatatggat aaacgcacca tactgctcca cgcgctcgat catagctttg
    20761 atataacttt cttctatttt attttctgat aatagcggct gtgcggcaaa tgtgattgct
    20821 tcttgccagt tttttggttg atccgataat tggatcattt cttctgttaa taattcagat
    20881 agcataggac gttgatcctc cttcttttct aaaagtttgt tcattttacg atttaatact
    20941 tgatagagct tttcttcgtc tacgccttct tttaattcga tataaggcag tagcgccgac
    21001 aacaattctt gggcagaagg cattgaaaat cctggtatca gccaatctct ttgtacttga
    21061 ttgatcagct gatttttttc caagtcagtc atgattggat gcagaacata gagagacttt
    21121 ttcttatcag taagtgtgag aggtacagta gaaaaaataa tatcgtaaga agtttcgtca
    21181 acctcgtgta agcggtcagc tgaattagtc tctgtaaaaa agatcatcgg gaataattgc
    21241 tgaagttcac tttttaagat caatgaagag ctaatgccac ttggacagac gatcacggct
    21301 cttatttttc tatttctttc ttctgcgtcc gcttttctga tctctccgcc aaatagtata
    21361 gtgaaaaaac caatctcttc ttcagggatg ctcttcccta ttcgtctttc taacggaagt
    21421 aatgcttttc ttgtcatttc gtagatggaa gcatattgat gcttaatttg atcgatcatc
    21481 acattaggta aataaaaaga ataggaaata cgaaaatagg cagggaccaa gtgataaaag
    21541 acattcatca ataattcacg aaacgaagca aattctacgg ccatcaggaa ttccatgcga
    21601 tggatgactt ctgaagaaca ttccagcaaa aaatcaagtg caggctcctg gacatctcct
    21661 tcaacgatcg atgctaagca aagagagaaa aatataatct ccgaattttg atgttcggaa
    21721 tagccaaggc cagtcaatac ttgtcgacta atgtcagtga ctgcaagaga agccaacagt
    21781 tcagcatctt ttaccagttc catggatgtt tttccaattc gaatggttgt caaactcaaa
    21841 taataagcta attcatccat tcgatttggt gcgaatcgaa gatctgatag tgaaatcgtt
    21901 ttatctagca aatctctaac tttggcgtaa taatcaatgg aaagggttgc ggttaaatac
    21961 gtgagcccaa atcttccagc aagtgactgc atcaaacgag cagtgcgatt ttttgcttct
    22021 cttcttaata ttgcttctga ccctagcagc ctaaaaccag ttttcctagt atacgacagc
    22081 tgacaatctt ccacttctaa ttcatttctt atctttttga tatctgacag gactgtccct
    22141 ttagaaacct gaagaaaatc ttgaaaatga aaaacagaaa cagacgttgc agtcgaataa
    22201 accatcaaat aaagaagatc tcttcgctct tgttcggtat acacagcttc aaatcgagta
    22261 atatccaaaa atttttctgc ccagtcgatt gctcggaatt tctttatacg gatacgggta
    22321 tcatccacat attcggcaaa aggagttaat tgtttgatcg cctcgcttgt atttacttcg
    22381 atgatctctg ctgctttttc tagactgatt tctcttggtg ttttcaggag ataactaatt
    22441 ttgtagttca cagcgctgtt cacctcctac ttatattcta gtatggtagc gcttttaaat
    22501 cgaggggtgt ttggtacaac ttacagttgg atttttttta cgactgtacc gaacgatttc
    22561 tatcgaatgg atacagaaaa aaagcaggtg accaaatgtc tcctgctttt atgaattgat
    22621 ttcgtccgat caatgatgat catgtttatt ttttgaggtc agttcttcaa atttttcttt
    22681 gacatcttca accgcttttt cgatatcttt ttcaaagtga ttgggctcat taggctttgt
    22741 ttcctcttct acagaatttg aaccagataa agctgtttct ttattttctg tttcaccaga
    22801 gtttgtgcca gctaatgatt cttctttgtc ttctgtttca ccagaattag ttcctgctaa
    22861 agccacttct tcttctgttt taggggccat tgaacctctt aatcgtttgt gtttttcacc
    22921 gtcataattt gtcatgttgg ttcctcctag tagttctata ctttttcatc gtacaataag
    22981 acagtttatg cgtcaaaaaa caaacccgat tcttacaaat atgtcatatt tttccttctg
    23041 attcttgaat gcttttgatt ccttcttcca aaattttcat ttgtaagatt gtttcttcat
    23101 ctttgatgac tttttctgcg ccattaacta atgtctcgta aatcccttcg tagacacggc
    23161 tgtaatcacc aacctcggaa attacttttt cttcatgata actcccttgt tcatctatat
    23221 aggtcaatac accataatgt tcgggcaaat cgataccaaa gtcttcatga tcaggcatgt
    23281 aaaacatttt cagatgttct tcttgtcgat cttttgtttg tttcacgaac atcccttttt
    23341 ttccgtaaac gacgaaactt ggacgttctt tgatgcggaa ataactggat tttatcgaaa
    23401 ttttcatatt tccatagaaa aaatccagat cgaagtaatc attcattcgt cctttcccta
    23461 aaagttgacg tacatcatag ctgatatcat ctggttcgcc gaaataagaa agaacttgat
    23521 ctactgtatg acaagcatgt ccatataaat agctgttcgc agtagaaaat tcatgcgtat
    23581 tttctggtac ttccggtctg aaataatcat aatgcatttc tacttctaaa atatccccta
    23641 attttccgct ttcgattact ttttggactg tcaagaagtc agaatcgaac cgtctgtttt
    23701 ggtaacattg gataaataga cctttttctt ttgctaatgc aaataactct tctgcttcct
    23761 ttgacgtttc tgcaaaaggt ttctctacta gtacattctt accatgttcg agtgccattt
    23821 ttccatattc ataatgaaat tgactaggtg tagtgatgac aactaattgg atctcaggat
    23881 catcccaaag atcagacagc tgatcagtat attcgatgtc ttcgaatgtt ttccacactg
    23941 gttcagatgg ggaaaaaatt tttttgatat tgattttgtt cgtaagtttt cttgaaaaag
    24001 gcaaatgata gcgatttgta ctctttccat tgccaagata agcgattgta agcatgagtg
    24061 gtgcctcctt ttattgatgt tttcagtata tcctagaaaa gcgcagaaag tatttataat
    24121 agctattctg gtaaaaaagt tcagaaaaac acttgttatc attcggtttg gtgaaattaa
    24181 tgttcaaagt aaggataaaa atcaaaaaga tagtagcgga accaagtgtt tatcagtaag
    24241 atagaatttg gaggtcagat catgcttatt gaagaaaaac taacaaaaca agagttgttt
    24301 accacgacag agaaacggat tgtcgattac atccgtagga atatcgaagc tgcagtctac
    24361 atgacgattg aagaattagc aaaagctacg tatacttccc attcagctat cattcgttta
    24421 tgcaaaaaaa tgggattcag cggatttaaa gaattccgtt tcgagctggg gcgggaagtt
    24481 catcaattga ttgctcagtt caatcaaacg attgatccta attttccttt ttcaagtgaa
    24541 gatagcactc agcaaattgc aaagaaaatg gcggatttat cgatccagtc aataaaaaaa
    24601 gcacaattgc agattgaaca tgaagattta gatcaaatcg gtaaagtatt gaacaaggca
    24661 cagcgaatct ttctttttgc aaaaggtgat tctcaaatca cggcaaggaa attccagaat
    24721 aaaatggtca agttgaataa gtttttgatc atggctgaag aatacagtga ttcttcttgg
    24781 aatgcagcca atttgactag cgaggattgc gccattttta ttagctacag cggtcgcatc
    24841 catcattatg aacgaataat gacctatttg aagcatgtag aggcccctac cttgctgatt
    24901 accggaaatc agcattcaga aatggccaaa caggctgata tgtgtcttgt tatatcgcag
    24961 gaggaatatg attttgctaa agtagcgact ttctcctctc aaattgcctt cgattacgta
    25021 ttaaatacgt tattctcagt gatatattct caaaattttg aagagaatat ccgcaacttg
    25081 aaaaacaaac aagaattgat gcaaaaaggc cttcttaaag aatatcggaa aacttaagaa
    25141 aagatcctca tttagtaata agaaagatag tgtttgatag gctctctttg atatcatcat
    25201 aaaaacaggc tgaagttttc tttttcaaac ctcagcctgt ttgtttacgg aacgattcga
    25261 atagatatat tatagaaatg aggaatagct aacttctgtt tcttattttg aacgattgac
    25321 gaaaaagata tataagaagg aggaaaatca gtatacatgc agctaacaat ccatacagga
    25381 aaaatcgttt gttctcggat actttctgta cttctttttg aacttctttt gcatgataag
    25441 gaacccggtg ccctctgaca agtaagcgat gactgttgac catataaggt gtacaagtta
    25501 ggagtgtcac gaaatcctgc cctttttcga ttctcagatc ttttgtatca gtcggttcta
    25561 ccgtcttgat ctgatcgact tgataagcga gtgtccgtcc attgatttcg atatagaaag
    25621 aatcacctaa agtcagtttc ggtaagtctg tgaacaactt tgcttgaggc agtcctcgat
    25681 ggctggaaat aacggcatgt gtattttctc cccccgtagg ataagacgtt ccttccaata
    25741 atgaggcacc tttttctagc agcacagtat tcgtttcatc aaaaattggc aaatggacat
    25801 tgatctcagg gatcgtcaaa ataccgatcg tatgtttctc aaaataggtt ctatcctttt
    25861 tcgcaggttc ttttttctta gtaaagggat ctgcacctgg atttccacct tcttttgcta
    25921 attgctggtt tttctttgtc atgttttctt ggattttagc catgacttct tcattttctt
    25981 tgaccgcttg ttgctgataa tgagagatga tctgctgatc cagataatta ttcagcgcgt
    26041 cgctaacaaa tggataagct agtatcccta ttcctgagag aagcaagaaa agcatcagca
    26101 gatctagaag tttccttttc tttggtttca tttgtgttct ccttgtgatg actgtttgat
    26161 aataaaagaa aaaggagcat ttgcgtaatc cgctgctcct ttttccaatt cttgcaaaaa
    26221 gactaaatct gactgtgctt gcgtctagca aagtacagtc cagcaatcag tagaaggact
    26281 actcctgcac cgatatacac atagattccc ttaccgcctg ttgaaggaag tgtacctttg
    26341 tgtttatttg gtattttttc aggagaaatc aactgtcctg ctgttacata agattgttca
    26401 tcgatagtaa atgctacacg gtttgttaat ggaacatatt tttctggcgc tttcgtttct
    26461 tccagatagt acgtgccata ttttagacct gtcacatcga ttaaaccgtt acttgtggtt
    26521 gtaaaaaccg ttgctgattc tttcgccgat acccagctga cggcttttgt ggatgggtcg
    26581 atcgataaat atttcgctgt gtcactatct tgatcacgaa cgacgaattc tgctccagca
    26641 agtgtttggt ctgatgtaac gtcaccatct actttaacaa atcgtttgcc cccagtaacg
    26701 acatcgactg acggtggtgt ttgatcattt gtatgcccgt tatcgacatt tgcttggtta
    26761 gaaaatcctt tggttggatc tgctttttcg ttcaaatgca tatagtaaac gaatttcaat
    26821 gtaccgccag gagttaatga aggaatataa ttcggatcaa ctgaaaccgt gaatccgtct
    26881 gtttgctcag tgacagaata atttactggg tcgatttctt tatttccatc atataaggca
    26941 taagcatacg ttcctgaaga atcattatca aatgttaaag cagcgtcatg agtatcgata
    27001 agtttgaatg ttgtgtactt gttttgcgtt ccttctttat cagcgatacc taatgggata
    27061 ttgacagaaa tctcatattg aattttttca ccgattgcga catcttttcc attcaattga
    27121 ggtgttgttt tatcaacttt agacgtatca tttttgatgg tcttttctac tggtgtttgg
    27181 ctatttgcca ggatctcaaa aggcgtgatt gtttgctttt caatcatttc cgcattatca
    27241 ggagctttta cttcttctaa attatatttt ccaacttcta aatgattaac gatcaactgg
    27301 cctttttcaa tagatgcctc ggcaaagtca ttgttgccga tgtcataaga atgaccagta
    27361 atgaaatgtt tggctttggt ttgatcagtt gtccaagtgt acaatccatc tgtgacactt
    27421 tggatgtatt ttttgacgct tggtgttcct tcttctttag aaataataaa ttctgctcca
    27481 tttagtgctt cgttttcggc agtaccgatt tttgtaactt tcaacgttcc atcattaccg
    27541 actgtatttt tagggtagag atggatagta tctagttctt ctgtcccgta tttataagag
    27601 ccatctgctt gtttgatcat ctcatataca gggaaagcta aaaccatgtt tgcggcagct
    27661 gacactccgt cttttggttc ttctttgatc gtgtagactg catctttccc attttgtttt
    27721 ttaggtaaag ataaagtgac atttccatca gcatctgtcg ttcctgaagc aactggtgta
    27781 ccaggagtca aagactggac tgcttgtttt gctgcatcca cggacgctcc tttatctcgt
    27841 tgcgcataaa attcttgagt gacgttataa actgaaaatg aaatatcggc taatccttgg
    27901 tattgatcga attcgctcat ttctttcccg ctgttttgga ttaaaggatc gggtaaatca
    27961 gtcattttct ttttatgaag gatgacttga gctgctgtct cctctgcaaa cacttttttt
    28021 gcgccgaagc tgtttgtgag taatggtaaa ataaggaaaa ggactcctaa cataacgttt
    28081 atttttttat gatttttcat tttttcactc ctgttctttt tttgataaac aaccagattg
    28141 caaagcttgc gaataccata ccaacaacgc ctaaaagtgt atttttcgtt tcgtttgttt
    28201 ttggaagaaa tggacggcta ttttcttttt tattgactac tttcaatata ctgtccgcct
    28261 ctttatcctc agaaagaaaa gttgaaatgg taaacggaat ttctgtttca cttaaaatat
    28321 aaccagaagg tgcttcgatc tcttttaatc gataacttcc tgttttcaac ccggaaattg
    28381 aaaaagcgcc atttttatcg gaaatcaggt gaagcgcatc tgattcgttc tttgtccact
    28441 tgtatccgtt ggctgtttgc tggaggtaat ttcctgcttt gtttttaata aggaatactg
    28501 cgccagctaa tcgtttatct ggtttatttg tctcgatttt gacaaattgc ttactccctg
    28561 tatatacaga gactttcttt gtactgacac caaaaccaaa atccagtgtt ccgttgttgt
    28621 tgatttcctt gttcgcctgt gctgtactgc ttagctgcat ctgataagaa atggtcagtt
    28681 tttttccagc atatttttcc aacgaaggga tcgaaaataa aacatcaaat ccgtgaggtt
    28741 tcttgtgtat gacaaagcct tctgtcagct tttctccatt cgatgaaatc gttaaactat
    28801 taggtaaaaa cgttaatgct tcatccgcac tatctgacaa ttcgaacttt tgatagtcaa
    28861 ggatatttac aggaattgtc gtatgcaagg aataagtgat tttttcacca atcgtaaaat
    28921 catttctagg ctcttctaat accttctcaa aaggtggatc agggtagtcg ttttcttcat
    28981 ttttaggata caaatggata gttgaaagtt tttgattgtt ttgtccatgt acaggaagaa
    29041 caactaccat attctctgcc ttttctttga cgacttctgg tactttggat tcaatgaaaa
    29101 gatacgcttt atctcttttt tcttgatctt tagcggccag agaaaaagaa gcgataccat
    29161 cttcgttgtt aagagttgta gttgttgctt ctgcggtaaa cataccggaa gacgcaccgt
    29221 tttttgcgat ctctgcttgt gcttcttcta ccgttttgcc cttttcccgt agatggtaaa
    29281 aagaatctgt gacatcataa acttggaatg tgacaccatt taatcctcga tacgtttgta
    29341 ataaagcttt ttcttgtccg tcatttggat gatttttcgg cagttgcccg tttgggaaaa
    29401 gcaatttgtg taaagtaatc tgaaccattt ctgtttctgt ttctgttgct acctgagtaa
    29461 aagctggttt aaatagtaac aagaagagac acatactaag ccaaccaagt tttttcatat
    29521 ttagctgccc cctctttctt ttttcaaaaa aagataccaa atagaaaacg cacaaccaat
    29581 catccctact agatagattc ctaaacggcc aattcctccc gtttcaggta atggtacttt
    29641 tgcctgattc gtgatgtcta aagaaatctg gttgtttttg gctcctggtg acagaacaga
    29701 ttcatgatct tgtccatcca cttgaattga cccatcttcg tgtataacta tagtaactgg
    29761 ctcttttaga ccttggtatc cttctggtgt aaaagtttcg gtcagcgtat aagttccagg
    29821 ttttaaattt tcgaatagaa aggtatctgt ttcttgtcca tcttttggcg attctaagtc
    29881 catttctggt ccctgcagtc tgaattttgc tccttttaat gtctgtccgt tgtcttcttt
    29941 tttgtgtacc gttagatcaa acgcttttag tcgattttga tgagtgagcg tccatacatt
    30001 atttgtcatc tcggtatttt ttcccgtgta aatgattttt ccatatttat cgatttggaa
    30061 ttcataattt cccggcgaag tatcatatcc ggcaggacca gctgtttcaa cgactcgata
    30121 tttaccaggt gtgagtgaat caaaactggc aagacctttt tcattcgttg tttggctgtc
    30181 aattggctga taagtaccat ttgcttcttt tttctgtagg gaaaatgctg ctcctttaag
    30241 atttatctct ttgtccgtcc cttgcatcgc atacttacgt actgctacag gaacttcaac
    30301 aaaaggattt tcaatagtca actggatcgt atcatcggaa gtagtgactg tttttccatc
    30361 aatggttacc gtaccatcag aagcgatctt gatttcccaa gtagtctttt tgcttaatcc
    30421 atgcccttct ggagcttttg tttctgtcag cgtataggtc atttcttttt gcaatttgac
    30481 attttctgga agagaatagg ttccgtcgcc atgatctgtt agtaaagtat caatagaatc
    30541 tcctgttaat ttgaaaacag cgccaataag atccttttca cctgtagagg aatttttcgt
    30601 tattttcaag tttaacggtg tgaattgttt agtatttttc catgtcattg catctatttg
    30661 ttgagaatcg tatcctggta caggtaattc tttgattgtt tggtaactga atgcttgacc
    30721 ttgattattg tattgaggta gtgataatat ctcttgataa ctttctccgc tatttgcaga
    30781 taatttgtca atgtctgcac gttcccacgt atttgttgta tctttagctg gtttaatgat
    30841 tcgaatatat ccgtttttcc aagctgcagc atttgtcgta tgttcccgtt gaatctcaaa
    30901 agtaacttga tctggacgat cagctagatt gttgtcaaat tcttcccata actttttgat
    30961 gtgaagactg actccgggag ctttagcaga tggtataccg aattcagcta attcattggt
    31021 atcaatgttt ggctggaaag ttgtcctgcc gttcatttga taccagaaat ttggatggaa
    31081 gtcctcattt tctgtttgga ttctcacttg gtaatggatt tggatttctt ggtcttttcc
    31141 taaatagatc tgattgctct tgatggtatt tccttctatg gaaatagatg gagtgaccgt
    31201 tgtaggactt gtccccacac tcttgactga aagtgtacca ggctgataaa tgaatggttc
    31261 tgcgattgga tcatttattt gtccattgct tacagttgct gagatctgta cagctttttt
    31321 ggctaggtat tcagagatat ctgttgcatg atcagctgat tcatagtaaa gatcgccttt
    31381 ttcatctgat gaaaccattt gtcgcatacg agactctact tctgcttttg agagaccagc
    31441 tgccggatcg ctttgaagtt ggatgccaag accatgtatt tcgattcctc gttctttgag
    31501 tgccatcgct tctccgatgg ttgcgataaa cgtactgtcg attcttctgg atagattgtt
    31561 ttggtcaggt gcatcataga ttcttgagat tagagaagta tttcccggcc gatcttgcgt
    31621 attagaaaac tgagttccgt aataattgct gcttgattgt gcgtgtaccc gctgtacttt
    31681 ataggaaaat gttggtacac catccgtcag caaaacgatc acttttttat gtccatttgg
    31741 aacggatagc atgcttcctg catctcttag tgctttttgt gtaaaagtac caccatttgt
    31801 ccgtgaaggt gtaatggatt ttacttgatt tttcactgaa tcaaatgaac ccatctgtac
    31861 tgcaccgtta ctgtagctat atccttcgct tgagtagccg acatatccca tattgatttt
    31921 gtctgtgata ccgctatctg ctaaagtatc gacaaaacga tcgacaccaa tctttacttc
    31981 accgatccga ttattgtcgt tcatacttcc tgaccagtct acgaccaata cgagatcaag
    32041 aggcgtgata tctttttgta catttccacg agcattcaaa taaacatcaa acaatccggg
    32101 tgtacttgtt tcctttgcat actttcgtat cgcatagtct gcttgattac cggttcctcc
    32161 atattcgata tacgaatggg tccgatcatt aggatctcca ttccaagaat ttataccatc
    32221 ccattgattt gttccgtttt tattgccttg atgatcaaga acatttgtat ttcctgtagg
    32281 ttgccaactg gctttaggat aagtgccctg atcatctgtt gtatattctg taggaatcgt
    32341 ttgattagat ggcacactca cagctggcaa ggataatgta ggcaatgtaa atgggctgac
    32401 agaattttcg ttttctttta gatgattctt cttctatttc gtctggcttt ggtactgaca
    32461 catctgtatc tttcttattt tctgtctcat caataatgtc atattcttta ttatcgacga
    32521 tcagcttgtt tgcctggctg tggtaaagtg ctggatcgat tggaaaactc agatcgatag
    32581 tttgtttagt gtttgatgag atttccaacg taattttccc gctatttacc tgataagcat
    32641 agccatctcc ttcttttctt tccacatcac ttaagtaatt ttcgactaga ccaatttctt
    32701 tttctatctt ttcagcactg gtgttattcg catttagctt gatgttcagt gctttttttc
    32761 cagactgatc cgaaatgact tcagctgaaa ttgtttctaa aactgttgga tcatctgtag
    32821 tgtctgctat tgcagataat ggcacaaagt ttgataaact caatattatc agcatgaaaa
    32881 taactttcag tttcttccct gtggtcgtca tttttcccct cctttttctt tttttctgtt
    32941 ttgacgtgaa tagtgaaaat tattaaaata aacaggcgtt acataatttt atttatttta
    33001 atatgataat tattatacga agggcgctaa agggaatcac accaattttc ataccaattg
    33061 gtttcattta tgaagttgat tccttcgatt aatgagcgtt tcattgaaag aaaagggttg
    33121 agggagaaaa acatatgatc aggttgttgg aattaaacta tagaaagatg gaaaaaatca
    33181 ttcgaattct gtccatttct gacaagtgga tgactaaaaa agaacttgcc aaacgaattg
    33241 gtagttcaga atctacattt gtaaggtata tcgaagagat caaaatccgc tggggaacgg
    33301 atgtaacgat tcaatcttca caaaaattgg ggtatcgtct tgaacatttg aatgtaccag
    33361 cttatttaaa tgttctgatg gaaatgtcgc aaagttctac aaacagtcgt ttattaaatg
    33421 agattattgt caatccgggt aaaacgattg agtattattg tgaaaaaatt tccatcagcc
    33481 ggtcttcttt tacacgtaag ttgaaacaat gcaatcaaat tttagaaagc tatttattaa
    33541 aagtgatcgt tgaccaaggt tttcagttaa ctagccttaa aagtgagctc cagttgcgta
    33601 tctttgttac ctttttcttt ttaagttact acggaagacg cacgttgcca tttccaatcg
    33661 aaaaaaagaa gatcaaagaa attatggtca agaataattg tcagatgaat ttgtttcagg
    33721 aagaaagccc atatgagcaa tctttcttta ttatgtattt tgttgtgcat ctcatgagag
    33781 aatcccaagg aactccattt gaatggaaag ctggagactc ttatgtacag ttgataaata
    33841 tcgataaaaa agacttctta atcttgaaaa atctcttagg aaaattgaag tacagcagct
    33901 atataaaagt agtgaactat tttgtcaata cgattttttc agaattttcg gagaaagaaa
    33961 aaaatgaaat ca
//
LOCUS       DACGLW010000023        33817 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.023, whole genome shotgun sequence.
ACCESSION   DACGLW010000023 DACGLW010000000
VERSION     DACGLW010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.023"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            299..1960
                     /locus_tag="IXT53_001128"
     CDS             299..1960
                     /locus_tag="IXT53_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ4904969.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(2030..2377)
                     /locus_tag="IXT53_001129"
     CDS             complement(2030..2377)
                     /locus_tag="IXT53_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HAQ4904970.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2461..3402
                     /locus_tag="IXT53_001130"
     CDS             2461..3402
                     /locus_tag="IXT53_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ4904971.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3403..4446
                     /locus_tag="IXT53_001131"
     CDS             3403..4446
                     /locus_tag="IXT53_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ4904972.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4452..5522
                     /locus_tag="IXT53_001132"
     CDS             4452..5522
                     /locus_tag="IXT53_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4904973.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5512..6465
                     /locus_tag="IXT53_001133"
     CDS             5512..6465
                     /locus_tag="IXT53_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ4904974.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6477..7487
                     /locus_tag="IXT53_001134"
     CDS             6477..7487
                     /locus_tag="IXT53_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ4904975.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7772..8593)
                     /locus_tag="IXT53_001135"
     CDS             complement(7772..8593)
                     /locus_tag="IXT53_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HAQ4904976.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8690..8968)
                     /locus_tag="IXT53_001136"
     CDS             complement(8690..8968)
                     /locus_tag="IXT53_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4904977.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            9236..11245
                     /gene="metG"
                     /locus_tag="IXT53_001137"
     CDS             9236..11245
                     /gene="metG"
                     /locus_tag="IXT53_001137"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HAQ4904978.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11724..12311)
                     /locus_tag="IXT53_001138"
     CDS             complement(11724..12311)
                     /locus_tag="IXT53_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4904979.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12486..13808
                     /locus_tag="IXT53_001139"
     CDS             12486..13808
                     /locus_tag="IXT53_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ4904980.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13963..14739
                     /locus_tag="IXT53_001140"
     CDS             13963..14739
                     /locus_tag="IXT53_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAQ4904981.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14732..15301
                     /gene="rnmV"
                     /locus_tag="IXT53_001141"
     CDS             14732..15301
                     /gene="rnmV"
                     /locus_tag="IXT53_001141"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HAQ4904982.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15294..16178
                     /gene="rsmA"
                     /locus_tag="IXT53_001142"
     CDS             15294..16178
                     /gene="rsmA"
                     /locus_tag="IXT53_001142"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HAQ4904983.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16629..17258
                     /locus_tag="IXT53_001143"
     CDS             16629..17258
                     /locus_tag="IXT53_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4904984.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17382..18491)
                     /gene="ugpC"
                     /locus_tag="IXT53_001144"
     CDS             complement(17382..18491)
                     /gene="ugpC"
                     /locus_tag="IXT53_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HAQ4904985.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18700..19122
                     /gene="mgsA"
                     /locus_tag="IXT53_001145"
     CDS             18700..19122
                     /gene="mgsA"
                     /locus_tag="IXT53_001145"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HAQ4904986.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            19445..19981
                     /locus_tag="IXT53_001146"
     CDS             19445..19981
                     /locus_tag="IXT53_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAQ4904987.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(20021..21784)
                     /locus_tag="IXT53_001147"
     CDS             complement(20021..21784)
                     /locus_tag="IXT53_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4904988.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21787..23502)
                     /locus_tag="IXT53_001148"
     CDS             complement(21787..23502)
                     /locus_tag="IXT53_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4904989.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            complement(24281..24505)
                     /locus_tag="IXT53_001149"
     CDS             complement(24281..24505)
                     /locus_tag="IXT53_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4904990.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            24705..27473
                     /locus_tag="IXT53_001150"
     CDS             24705..27473
                     /locus_tag="IXT53_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ4904991.1"
                     /translation="MLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNTVSH
                     YLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQPKDL
                     FSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLHDLL
                     EDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLDEVH
                     RLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRIPIR
                     IELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGELKNI
                     VKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLMEKQ
                     QPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHELLVY
                     WTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELEQQV
                     SQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIAAHG
                     YATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILVDMG
                     SLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPEWRL
                     LYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNSDPL
                     FSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQRII
                     RNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLIERL
                     IRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSEDTE
                     FLSIEEEF"
     gene            27554..27973
                     /locus_tag="IXT53_001151"
     CDS             27554..27973
                     /locus_tag="IXT53_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HAQ4904992.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            27985..28455
                     /locus_tag="IXT53_001152"
     CDS             27985..28455
                     /locus_tag="IXT53_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ4904993.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            28472..29251
                     /locus_tag="IXT53_001153"
     CDS             28472..29251
                     /locus_tag="IXT53_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ4904994.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            29238..30059
                     /locus_tag="IXT53_001154"
     CDS             29238..30059
                     /locus_tag="IXT53_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ4904995.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            30076..31125
                     /locus_tag="IXT53_001155"
     CDS             30076..31125
                     /locus_tag="IXT53_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ4904996.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            31138..32142
                     /locus_tag="IXT53_001156"
     CDS             31138..32142
                     /locus_tag="IXT53_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ4904997.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            32775..33794
                     /locus_tag="IXT53_001157"
     CDS             32775..33794
                     /locus_tag="IXT53_001157"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAQ4904998.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT        11042 a         5991 c         6908 g         9876 t
ORIGIN      
        1 tcagataagg gactgtgaca agacttttgt cacagtttct ttttgtttat tcttttttat
       61 ctttgtatgg caataataga caatataacg aaaattattt aatggctttt ggaagaccat
      121 ggaaaacctt gtacaaagct attttttctg aaaaattcct tgaaacctca cgaaaaaatc
      181 attattttat gaaaaagaca aaaaaaagtg ttgatttttt aataattatt cattaaactc
      241 ttaaacaact tcagaaaata ttaattattc tgaaaattcc aataaagggg tacgtaaaat
      301 gaaaaagaaa tttacatttg gtttggttgc ggtatgcggc cttgtacttg ctggatgtta
      361 tggcggcagt tcagccacaa gcagtagttc agcaaaaagc ggttcagcag atggaggagg
      421 cgtatttaac ttagtcgtgc ctcaagaaat gcctacagct gacttatcag ttgcaacaga
      481 tacaatcagt tttacagcat tgaataacgt ttatgaagga atttaccgtc ttgatgaaga
      541 cagcaaacca cagccggcag gagctagcga gttggcagaa gtcagtgaag acggattaac
      601 ttacaaaatc aaattacgag aagacgcaaa atggtcaaat ggtgatcctg ttacagcagc
      661 ggattacgtt tatggctggc aacgaacagt agacccagct acagcttctg aatatgctta
      721 cctgtatgcg ccagttgaaa atgcagaaga cattacagct ggaaagaaag ataaatcaga
      781 actagggatc aaagcagttg gtgactatga attagaaatc aaattgacga aacaaacacc
      841 atatttccaa tatctgcttg ctttcccatc atttttccca caaaaacaat ccgtagtgga
      901 agaaaatggg gatgcctatg catctgcaag tgataaagct gtttataatg gaccattcac
      961 attagaagat tttgatggac caggaacaga tacagaatgg acttataaga aaaatgatca
     1021 ttactgggac aaagatacag taaaactttc tgaaatcaaa gtcagtgtag taaaagaatc
     1081 ttctactgca ttgaacttat tcaaagatgg ccaagcagat gacgtgatct tgtctggtga
     1141 gttagcacag caaaacgcaa acgatccagc ttatacttct gttaaagaag cgcggacaag
     1201 ctatatcgaa ttcaatcaac gtgaaaagaa ttcaccattc aacaatgtga acctaagaaa
     1261 agctatttct tattctatcg atcgtgaagc tttagtaaaa caagtactag gtgacggatc
     1321 agttgtctct acaggattga tcccatcaga tatggtaaaa agtccaacaa cgaacgaaga
     1381 ctttgcaaaa gaagctggaa aattagtttc ttatgacaaa gataaagcaa aagaatattg
     1441 ggaaaaagcg aaaaaagaat tgggcgttga ttctttagaa tttgatttga tggcttctga
     1501 tgatgattca tcgaaaaaag taatcgaata tgttcaaaac tcaatccaag aaaacttgga
     1561 tggcgtaaca gtcaaaccaa cacctgttcc attctctgta cgtcttgacc gctcaacttc
     1621 gggtgatttt gacgcagtac taggcggatg ggctgctgac tatgcagacc caagcagctt
     1681 tactgatttg tttgtcacag gtaattcata caaccgtggt cgctggagca atgcagaata
     1741 tgacgaagca gttaaagcag cttcatcaac agacgctggc gatgaacaag cccgttggga
     1801 tgatttccaa aaagcaatca agatcatctc tgatgatatg ggtgtcgttc cggtgtacca
     1861 aaaagcagaa ggtcacctag tgaatgaaaa aatcaaaggt atcgttcacc atgcggcagg
     1921 cgcttcatgg gattacaaat ggacatacgt tgaagaataa tcgttaatac cgtgagcatg
     1981 agaaaaaacg aggctgcggc aaatgtcgca acctcgttct catattcact tatcctaaca
     2041 aagaaaaaag catgaaaata gcagaaagag caattagtat tccggcaatg atcagccaaa
     2101 agctgtacat accgtagctg gcagacgaga gcgaggaaaa ttctggtttt ttctttcggt
     2161 tccttgttcg tgcaagatgg accgatcgct gaaaatgatc gaagaaaccc gaagaaaaga
     2221 cccataaaaa accgccgata atcagaaacg gcaatgacca taagaataga tcattagata
     2281 attgaatgag tgtaaaggaa tggttgatga gattttttat taaaataacc agaatcagta
     2341 gaaaagctac tgcgtagggg atagattttt ttttcatttg attgctcctt tatttttggt
     2401 tcattatcta ataatgtagc gaaacatctg tcagaagtca aaagctaggg ggaaacatta
     2461 gtgaatagtt atatcaaata tgtattaaaa cgtgtctttt tcatgatcat cacgctttgg
     2521 ttaattgcaa caatcacctt tttcttgatg caattgctgc cgggcacgcc atatacgaat
     2581 caggaacgtt tgagtcctga aacaatcgag atgctaaata aacaagtcgg tttggataaa
     2641 ccagttatcg tacaatatgg gatttatctt tctaatttgc ttcaaggaga ttttggaatt
     2701 tccttccaat ttaaaaacca gccagtcgca catttgttag ctggaagaat cggaccatcc
     2761 ttgcagttag ggttacaggc aattatcttt ggaacagtcc tagggacgat tttaggaacg
     2821 atctctgcca tgaagcaaaa tacatgggca gatacatctt ctacactcgt agcgatatta
     2881 ggtcgttcca ttccaaactt tgtatttgct gttttattac aatatatctt tgcaatcaaa
     2941 ttacaagttt tgccaatcgc aaaatgggat ggctttgtgt acacaatctt accaacaatt
     3001 gcgttggcca tgtcgccgct tgctgactct gctcggttta tccgtacgga aatggtggaa
     3061 gtactgcata gtgattatgt ggaattagca aaagcaaaag gattgagccg ttgggaaatc
     3121 gcctttaaac atggtcttcg aaatagtttg attccattga tgactttatt aggaccgtta
     3181 gctgtagcat tgatgaccgg atcactggtg gtagaaaata tctttgctat tcctggtatc
     3241 ggggaacaat tcgttaagtc aatcacaaca aatgattatc ctacgatcat ggctgtaacg
     3301 atcctttact catttatgct gatttttgtt attttgatcg tggatctatt gtacggtcta
     3361 gttgatccaa gaattcgtgt ttcagaaggg agccgaggat aaatggaaat gataccaaag
     3421 cgttatgaaa cagttgcaga tatccctgcg gatgaatttc ttccattaca aaaaaatacc
     3481 gaagaagaac gtgaacagat tactgcacct tctttgaatt ttatccaaga ttcatggcgc
     3541 cgtttaaaga aaaacaaagc tgccgttatt tcaatggttt tactattggt catcattttg
     3601 atctctgtga tcacaatctt tgtttctcct catgatccaa ctgcccaaaa cgtggcttat
     3661 atcaatttgc ctcctcgtat tccgggaatc aatatcgatg gattgaatgg aaaagcaatg
     3721 gttggtggtg aacttgttga taaatatgct caagcaaacg tcccttcaaa cgtaaatttc
     3781 tttttaggaa cagacggttt gggacgtgat gtattaagcc gcttatttat gggaatgaga
     3841 atctcgttgc tgatcgcatt tattgctgca ttactagatg taacgattgg tgtagcttac
     3901 gggttggttt ctggtttgtt aggcggacgt gtcgataacg tgatgcaacg ttttttggaa
     3961 atcttatcag gaattccaaa cctcgttgtc atgattttga tgttggtcgt ttttgaacca
     4021 ggaatttttt caatcgtggc tgctatggcc atcacaaact ggataccaat ggcacgtatc
     4081 gttcgtgcac aaacaatgaa attaaaagat caagaatatg ttttagctgg attgacatta
     4141 ggtgaatcaa gaatgaaaat tgcattcaaa catattttgc cgaatatctc tagtgtcatt
     4201 attattcaaa tgatgtttag tattccatct gctattttct tcgaagcctt tttaagcttt
     4261 atcggtttag gtcttacgcc tccgtcagct tcattaggaa cgatgttgag cgaagggtac
     4321 aagacattcc tgtatctgcc ttatctatta tggattccgg cagtaacttt atcggttatc
     4381 atgattggct ttaacttact tgctgatgga ttgcgtgatg cctttgatcc taaaatgaaa
     4441 gagtgaagac gatgacgaaa attttagaag tgaaagactt agaaatctct tttgatacgt
     4501 atgctggaaa ggtacgagcg attcgcggtg tcaattttca tttgaataaa ggagaaacat
     4561 tggcgatcgt tggggaatct ggaagtggaa aatcagtgac gaccagaagc atcatgcgct
     4621 tgctgtcaag caacgccaat atagatgctg gtcaaatcct ctttaaaggt aacgatatcg
     4681 ttggaaaatc agaaagagag atgcaaaaaa tccgtggtaa agaaatagcg atgattttcc
     4741 aagatccaat gacctcttta gatccgacga tgcccattgg gaagcaagtc gctgagtccc
     4801 ttagaaagca taataaagtt tcaaaaaaag agggacaaaa agctgcatta gaattgctta
     4861 aattagttgg aatacccgaa gctgagaaac gtatcaacag ttatccgcat cagttctcag
     4921 gaggtcagcg tcaacggatc gtgatcgcga ttgcactggt atgttatccg gaaatcctga
     4981 ttgctgatga acctacaaca gcgcttgatg tgactattca agcacagatt ttggagctgc
     5041 tgaaagaaat tcagacaaaa atcgatacat caattatctt tatcactcat gaccttggtg
     5101 tagtagcgaa tgtcgctgat cgtgtcgctg tcatgtatgg aggaagaatc gttgaagttg
     5161 gaacatcgga agaaattttc tacaacccgc aacatcccta tacttgggga ctgcttggtt
     5221 caatgccgac attagatagt gcgaacgatc gattgtatgc tattcctggt tctccaccgg
     5281 atttattgaa cccgccaaaa ggagacgcat tctatccaag aaatgaattc gcgatgaaaa
     5341 tcgatgcaga acaagaacca ccattttttg aactatcaaa aacacacaag gcagcaactt
     5401 ggctacttgc tccgcaggca cccaaagtga caccgccaga agaaatccaa cgtcgctggg
     5461 ctatctttaa agagaaacaa aaacatactt acggtcaagg aggaattgct gatgccaaat
     5521 aaactacttg aagttaaagg attgaaacag tatttcaatg taggaaaaaa gaatgaagta
     5581 catgctgtaa atgacatcag ctttcacatt tatgaaggag aaacattcgg tttagttgga
     5641 gaatctggaa gcggaaaatc cactactggc cgtacgatca ttcgcttgaa tgaacctact
     5701 gatggtgaga tattattcga cggtcaagat ataacgaaga tcaaagataa aaaaggtttg
     5761 acgaaattcc ggcatgatgt tcagatgatc tttcaagatc catacgcttc gttgaatcct
     5821 cgtatgaaag tcagagatat cattgccgaa ggaattgatg ttaatggact agctaaatca
     5881 tctgaagagc gtgcaaaaaa agtcgatgat ctattgagaa ctgttggatt gaatccaagc
     5941 cacggtactc gttatcctca tgaattttca ggtgggcaac gccagcgtat tggtattgca
     6001 cgagcattgg cagtaaaacc gcgttttatc atttgtgatg aaccaatttc tgcactagat
     6061 gtgtcgatcc aagcccaagt ggtcaatttg ttgcaagatc tccaaaaaga acatcaattg
     6121 acctatctat ttattgcaca tgatttgtcg atggttaaac atattagtga ccgaatcggt
     6181 gtaatgcata acggcttgtt attagagatg ggaacaagtg atgaaatcta taatcacggt
     6241 gttcatcctt atacagaaag tttgttatct gctattcctc taccagatcc agaccatgag
     6301 agaaaacgca gaagaatcaa atatcaacct gaaccagatg acggacaagt acgtcaactt
     6361 cgtgaaatcg caccagaaca ttttgtctat gcaactgagc aggaagtccc atattacgca
     6421 aaaaaactaa aacgacaaaa ggaatctctt ctggttgcta actaggaggg acatagatgc
     6481 taaaagcttt taaattccgt atgtatccga cagaagaaca aaaacagcaa ttgattcgta
     6541 cttttggatg tgctcgattt acctataacc atttgctgaa aaagcggcag aaaagttggc
     6601 aacaaacagg agtagccaat ttttcgctca cacccgctac attaaaaaaa gaatacccat
     6661 tcttaaaaga agtagacagt cttgctttag caaatgcaca gcttaatttg gatcgggctt
     6721 ttcgcaatta tttcaaggga cgtgccagct tcccaaagct taaaacgaaa aaaagtatgt
     6781 ggcaatctta tacgaccaac aaccaaaccc gtacgatcta tctggaaaat ggctacttaa
     6841 agttaccaaa acaaaaagaa ctaatcaaga taaatcagca tcggccagtt gaaggatcga
     6901 ttcgctccgc tacgatttct gctcgatata atgaagaatt ttatgtcgca ctactttgtg
     6961 atgtttcgcc agtaaaaaaa gaatctttag ctaaatggat cgggatcgcc tatcatccga
     7021 agacattgat ccaaacatct cgacctctag aagtaacttt accaaaattt caccagacag
     7081 aagaaaagct gcaacatgca cagcggaaat tgaatgtgaa agtccgctcc gctcaccata
     7141 gaaagatcag gttagatcag gcaagcaatt atcagaaaca aaagagaaaa gtaatggatc
     7201 tttatctgaa acaaaagaac cagcgagaag attatcttga acaattatct gggaaattgg
     7261 tcaaacaata tgactactta tttgtagaat cttttccaaa agaagaggca catgctgatt
     7321 tttctattca cgactggcat aaattaataa caaaattgcg ttataagtcc caatggtata
     7381 ataaaaaatt tcttttgatc aataccgacg gagcagaaga aagcaattcc gtaagaaaga
     7441 gccaagtagt agaaaaaatg ggcagacatt cggttatcaa gggataaaaa atgaatgaaa
     7501 ctgataggag cgttagggtt agccttgtta ataaaaagtc ttaatgactt ttgtttctag
     7561 gaatttttct tttgaagaaa agctcactaa ttatgacaaa ccaataaaac aatagcagaa
     7621 aaagttcgta cgagcttttt ctgctattgt taatttttta ttcagaaagt ttacgaatac
     7681 atcaaaattc tgaaacaaaa aacgtaactc acaaagagtt gtttttgcgt tttttcttga
     7741 atgattaggt ctgaatcttt tgctcacaac cttattcatg gtgccccggc ttgagctgat
     7801 agatttttga aggacggccg attcctacag gacgttcacc gatctcatcg aaatattgga
     7861 gcatagcttt cttaaaattg gaatgatcga tcgttttata atcgatccct aagaatttag
     7921 caaatacttt tcgagcttcc gtaatcgtga aatctttacc taaaacttgt agaacttgcg
     7981 gctcgtgttc cattttattg actacacggt tgaatgcttt caagatgatt tgactatggt
     8041 caaaagccaa agtatctttg ccagaagact ctccagtgac taaatcaagc gagatagcca
     8101 cttctccgct agataaatgg atcttgttac tatgacgttc tagtgtgaac cagcgtactt
     8161 ccttggcatc atctccggcg attagcggtt cttctccaat aaaggctaag tagctgactg
     8221 tgacgaccca gcctcgcgga tctcgattag gcgtactgaa tgtatgcagt tgctcaatat
     8281 tttcttttga aataaccaca ccagtttctt cctttgtttc tcttaagacg ctttctccag
     8341 ttgactcatc cttttggaca aagccaccag gcaatgccca tgaatttcga taaggatgcc
     8401 cttttctttg gatcaacaaa actttcagct gatcttcttc tttgttatag cacatcagta
     8461 cgatatcgac agtcaaagag ggcttgtcat attctggacg ttcctgtttt tgataccagt
     8521 taagaaattc ttcttcactt gctttatgtt cataatataa tttttcttct tctttagaag
     8581 caaaacggtt catctgacca cctccattga cagtaaaagt actttttacc ttattgtagc
     8641 agactctgaa aaaaaaccaa ggatttactt gaaaaatcta ggttatcatt taaatatgaa
     8701 aaataaatgg aagtatgaga ttgaataaaa agaaaagcag ataaaacggc aatactttat
     8761 accgctcttc acgaatggca aagaagccac aaataacgat atatacgtca atgataaaac
     8821 gcaaaatcca agctgctgtc ataaaactgt tcaaatgccc atggaggtta aaataaccat
     8881 acatacgaat ataaaaatag ctgaatatta aatgtgctaa tgaaagaaaa gcaatactaa
     8941 gtgtaaaaaa aagatgtttc ttctgcatga ttgttccagt cccttactaa gatagatagt
     9001 ctttattata ccacgtgtga ggggagaaat aaaaagccgt tttttagtag atatgaaaca
     9061 aaacaaaaac ccgcatttat tcaacctatt catcctttta aaggatagat tgaacgtaga
     9121 tagaagtcgt ttatccagtc tttttcattt tgaaaaaaag ctaacagcat gcttttttta
     9181 tggtatacta gcaaggaaac gaattcgcat catgcgataa aggaggaaga gccccgtggc
     9241 agaaaaggaa actttttata tcactacgcc aatctattat ccaagcggca aacttcatat
     9301 cggcaactcg tatactacga ttgcatgtga cgcaattgct cgttataaac ggttgatggg
     9361 ctttgatgtg ttttatctca ctggagtgga tgaacatggt caaaaaatcg aaacaaaagc
     9421 agaagaacta ggtgtaaaac cgcaagaata tgtagataaa atggcggctg atgtcaagaa
     9481 attatggaaa acgcttgata tcagttatga taaattcatt cgcacgacag atgattacca
     9541 taaagcagct gttcaaaaaa tatttgatcg tttattagaa cagggagata tttacttagg
     9601 tgaatatgaa ggctggtact ctgtttctga cgaagaattc tttacagaaa cacaacttgc
     9661 agaagtgtac aaagatgacg aaggtaacgt gatcggtggt aaagcgccaa gtggacacga
     9721 agtagaattg gtaaaagaag aatcatactt cttccgcatg agcaaatacg cagaccgtct
     9781 attggaatac tatgaagaac atcccgaatt catccagcca gaatctcgta aaaacgaaat
     9841 gatcaacaac tttatcaaac caggattaga ggatcttgca gtttcacgaa caacttttac
     9901 ttggggagta ccagtcaaga gtgatccgaa acatgtggtg tatgtatgga tcgatgcatt
     9961 atccaattat attactgcac taggatatgg ctccgaagat gaaagtctat tcgaaaaata
    10021 ctggcctgca gatgttcaga tggtcggtaa agaaatcgtt cgctttcata cgatctattg
    10081 gccaatcatg ctgatggccc ttgatttgcc tctacctaaa aaagtatttg ggcatggctg
    10141 gctattgatg aaagacggaa aaatgtctaa atcaaaagga aatgtcgttt atcctgaaat
    10201 gcttgtggaa cgttatggtt tagatgcact ccgttattat ttgcttcgtg ctgttccgtt
    10261 tgggtcagat ggtgtcttta cgccagaaga ttttgtttcc cgtgtcaatt atgatctggc
    10321 aaatgatcta gggaacttat tgaatcgaac gattgctatg atcaacaaat attgtgatgg
    10381 actcgttcct gactatgcct cacttgtcac tccatttgat agtgaactgt ctacgacggc
    10441 tgctaacgtt gtcggacgtt accatgatgc aatggaaaaa atggaattca atacagcatt
    10501 ggctgaaatc tgggtgctga tttctcgtgc aaacaaatac attgatgaaa cagaaccatg
    10561 ggtattagca aaagacgaag aaaagaaagc tgaattgaat agtgtcatga tccatttagc
    10621 tgaatctttg cgtatcgcag cgatattact tcagccaatc atgacagaaa cgccagagaa
    10681 gatcttcaat caactaggat tggactctga aacaatgaat cttgaaggat tacactttgg
    10741 cgaatttcct tcaggaacaa aagtagttgc aaaaggtaca ccaatcttcc cacgtttaga
    10801 tatggaagaa gaagttgctt ttattcaaga aaaaatgtct gaaggtactc aaacaaacga
    10861 agatacagtc aaatgggatc cagaagaaac agaacttgtt tcaacgaaag aaaaacaaat
    10921 caaatttgat gtatttgaaa aagtagaact aaaagtagca gaagtcatca actgtcaaaa
    10981 agtagaaggg gcagataagt tgctgcaatt ccgactagat gctggagaca gccaagaccg
    11041 tcaaatcttg tcaggaatcg ctgaatttta tcctgatcca agcgaattga tcgggaaaaa
    11101 agtcgtcatt gtagcaaatc tgaaaccacg aaagatgcgt ggacaaatca gtcaaggaat
    11161 gatcctttct gcagaagcac cagatggctc tttacaagta atcgaagcac caaaatccat
    11221 gccaaatggc tcagaaatcg catagaaatt caaagggggt atgacactag ttgtgtcata
    11281 cccccttttt ccgaaatttg agaagcaatt ttcgtaaatt ccttcttatt acttgaagcc
    11341 aatcatataa tacagaaaca gagaactatc caaaataaat cctagaaaaa gaagtacgag
    11401 cttcgctgat ttttgttaat ttagaataat actaatagga atcgtaaaaa cagaaaaaaa
    11461 gcataatgat aaagggtcgg gcagatttta attgattcat ctggtactct tttttattgt
    11521 ccttacattc ataaagtttg aatgcaatga ctttattaca tctatattta tatctaacag
    11581 tagcgttgat gtgttaatta taagttctat cactttatgt tcaattagtt tgtgctctta
    11641 tatgtgtgat tgaaaatata gataaaaaaa caatcatcgg ctttgacttc gatgattgtt
    11701 ttctttattt tgtaaaaatc ctatcacttg attttatggt aaccttttat ttttgtcttc
    11761 aaacggcttg gtaagagagc agtaagcagt gcaaatggga tcgaaaagac ataccagaaa
    11821 atcgtacgtc cgataccata atgttgatag agctttgtca acatcacgtg taacaagatc
    11881 aaccctaaaa gcaaggagcc agctataaac caatgattat cccaatgcat gacttcacta
    11941 agtgccatca ctccaaaagg atagaaataa aaatagatcc cgactaaggt catccgccag
    12001 ttctttgtaa atgttccgat attcaataaa aagaaaatcg ctgaaagcaa aataccgata
    12061 ggcggaaaaa gcccaaccaa aaaggaatac aacatccccc acaagatcat cacaggacct
    12121 ttgatcaatg caacgattgc gatgaacaaa gtcagcaata ttagtttttg tgataacgtt
    12181 gcaacagtca ttaatccaaa taaaattaat gtgaaccaat gccaatggga ctgctgttgt
    12241 tctttacggg aaaaatcgac ccggttggat aaataatctg ctaatttcgg ataagaacgc
    12301 ttaaaatcca taaactgttc ctccttcgtg gaaaaggtag gtttagtata cgaaaaataa
    12361 aaaagaaata catccgtctt aagagggagt ttttcactga aagtggagaa aaaagaatgg
    12421 taaattaaaa tattctcagt tatactgaaa attataccaa atttcaaaga agaaagagga
    12481 tgggaatgaa aggcaaacta aaacgagaaa tcaatctgtt tggcgcatta gctaccgtta
    12541 tgggaaccgt gatcggtgca ggcgtcttct tcaaaactgc tgctgttact gctagtactc
    12601 agtctacaag tttgacattg ctagcttggc ttttgggtgg tttcttaacg atctgtgctg
    12661 gattgacagt ggctgaatta gctacagcaa tcccggaaac tggtggagct gtaaaatata
    12721 ttgaagctgc gtatggaaaa ttgcccagct ttttattagg atgggcgcaa agcttgattt
    12781 actttcctgc taatattgct gcattatcga ttatctttgc gacacaattg acgaacttat
    12841 tacaattatc taccgattat cttttgttga ttgcagtgat caccgctgtt tctgtgacag
    12901 ggttgaattt gttagggaca aaagtcggtg cttctgtaca atcagtgacg ttgattgtta
    12961 aattaatacc aattgctgtg attgtcatat ggggattatt gaccccagga caaggaactg
    13021 tccagttatt tccaatcgaa gctggaaaag atgttacttt tgtcgaagga ttgagcagtg
    13081 cgttgttagc aacgttgttt gcttatgacg gctggttagg tgtcggtgca atggcagggg
    13141 aaatgaaacg gccggaaaaa gatttgccta aagcaattat tcttggtttg agttttgtaa
    13201 cagtcgttta tctattgatc aattttgttt ttttgaaaac tttgccgatt gatcatcttg
    13261 ccggtaattt gaatgcagct tctgaagcat cagacgtcat ttttggagga atcggtggaa
    13321 agctggtcac tattgggatc ttgatctcgg tatacggtgc attgaacggt tatacactaa
    13381 ctgggatccg cgtgccttat gcgatggctt tagaagacga gctacctttc agtaaacagc
    13441 tgacaaagct ttcaaagaag tttacggttc cttatgttcc tgcggttttc cagttagcag
    13501 tagcttgtat tatgatgagc cttggttctt tcgacttttt aacagacatg ttgatttttg
    13561 tgatgtggct attcagttta ctgattttta tcggtgtctt tattttaaga aaaaaagcgc
    13621 cggaattgcc acgtccatat aaagtaccac tttatccaat cgttccgatt attgcgattc
    13681 tcggtgctat tttcatttta ggtatgacga tgatgacgca aacgaaatta gcattgatcg
    13741 gtataggtgt taccttgata ggtatcccag tctattatca aaaaaagaaa aaacgctcag
    13801 aagagtaaaa tatttcaaca acagacagaa aaagtacgaa ctaggtcctc tagatcttcg
    13861 tgctttttct atttcaataa aacaagttct tctattttga tttttgctac ttctgcaagt
    13921 agtgcttatg tgctaaagta agaatgaaaa ggagagataa acatgatatt tgattctcat
    13981 acccatttaa atgctgaaca gttcaatgaa gatattccag aaacgattca gcgagcacaa
    14041 gaaatggggg taacgaaaat ggcggtcgtg ggcttcgata cgccaacgat tgaaaagtct
    14101 ttgcaattaa gccatgacta tccaaatata tacagtatta tcggctggca tccgacagag
    14161 gctggcagtt atacgaaaga aatcgaaaat aaattacaag aacaattgac tttaccaaag
    14221 gttgtagcat taggggaaat tggcttggat tatcattgga tggaagaccc gaaagaagtc
    14281 caagataaag tatttcgcag acagattgct atcgcaaaag aaatgaacct accaatcagt
    14341 attcatacta gagaagcact tgaagacacg tatcaaatcc taaaagaaga agatgttcga
    14401 gatatcggtg gaatcatgca tagtttcagt ggtgattatg aatgggcaaa acgatttctt
    14461 gacttaggga tgcatatttc atttagcggt gtcgtcactt tcaaaaaagc attagacgtc
    14521 caagaggctg cagcaaatgt accaatcgaa cgattacttg tggaaacaga tgccccttat
    14581 ctggcaccag ttccttttag aggaaagcga aatgaaccag gctatactcg ttatacagta
    14641 gaaaagattg ctgaacttcg gaatcttccg acagaagaag tggctgccca aacatggaca
    14701 aatgcgcacc gattatttgg tctaagcgaa catggataag atgaagatcc aagagattat
    14761 agtcgttgaa ggaaaagacg atacaagaag gctgcgggaa gtagttgatg cagatacgat
    14821 agaaacaata gggtctgcaa tcaatgaaga gatcctgatg caaattgaac atgcccaaga
    14881 gactcgtgga gtgatcgttt ttaccgatcc tgatttttca ggagaaaaaa taagaaaaat
    14941 cattatggaa gctgttcctc aggcaaaaca tgcttttttg ccgcgcacac aagctaaacc
    15001 aaagaaaaag ggaagccttg gtgtcgagca tgcaagtgat gctgctattt tagaagcatt
    15061 gaaaaaggtc gtgacgccag acatttccca aacggctgtt tctgaaatca ctagacaaga
    15121 ccttttagac tatggtttaa tagctggggc acaagcaaaa gaaaaacgag aaaaactagg
    15181 tgacgagttg cgcatcggtt acacgaacgg caaacaattg gaaaaacgtt tgaagatgtt
    15241 tcgtattacg ccgaaagaac tcgcccaagc aatgaaaata gtggaggaac aacatgagtg
    15301 aatatcgtga aattgcgaca ccctctagaa caaaagagat tttattgaaa catggttttt
    15361 catttaaaaa aagtttagga caaaattttc tgactgaacc aaatatttta aggaaaatcg
    15421 tagaaactgc gcaaatagat gaacatacga acgtgatcga agttggacca ggaatcggtg
    15481 cattgacgga acaattagcc aagcatgcaa aacaagtcgt agcatttgag atcgatgatc
    15541 gtttgatccc tgtattggct gatacgatgc aaccctatga caatgtgact attgtacatc
    15601 aagatatttt aaagaccgat ttgtcaacag ctgttagaga aacatttcat gaggagcttc
    15661 cgttaaaagt cgtagcgaat ttaccttact atattacgac accgatcatg atgcattttc
    15721 ttgaatcaga tttggttgtg gatgaactag tcgtcatgat gcaaaaagaa gtagctgatc
    15781 gtatctctgc tgaaccagga acgaaagctt atggttcatt atcgattgct gttcaatatt
    15841 atatggaagc cagtttagca ttcatcgtac caaaaacagt atttgtccct cagccaaatg
    15901 tagattcagc gattttgaaa ctgacaagaa gagatatacc ggcagtcgaa gtgacagacg
    15961 aaaaagcatt tttccgattg acaaaagcgg catttcagca aagaagaaaa acgctttgga
    16021 ataacttgca gcattcgtat ggcaaagatg accagacaaa agcatggtta gcaaaaagct
    16081 tggaaacagc tggaatcgat ccaaaacgtc gtggggagac tttgtcatta caagaattcg
    16141 ccgcattaag caatgcgatg tcagaaaata tgcagtaaaa aaagaggttg tgacagaagt
    16201 tgtgaaaaag ctgtcctgca tcaagtaata agaacagcca aacaaaaata gttcctcatg
    16261 tttttcgttt gtttgggctt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
    16321 aggttgtggg cctgccgttt aactctaagt cacaaggaac aatttcaaaa aacagcttct
    16381 catgtttttg agttagttgg tcttattact tgatgcaagc ttttgaccac tattatgccg
    16441 aacaaggaga tgtgacacaa attttgtcac atctcctatt tttgtttctt gatcaaaaaa
    16501 ttcttatact tgatactcac actcattttg tatggattta gaaaagaata tgtgtcttga
    16561 cagttattag taagtgagtt atactaagtc gacatttatg tgtcaagaca tattaggaga
    16621 gagaaaaaat gagaaaaaat tcagttcgaa aactaacgat ttctgcttta ttgattggga
    16681 tgggggtcat tatcccaatg gtcatgccta aaatcatgat tccaccagct tcctttactt
    16741 tggcgtctca tgtgccatta tttattgcga tgttttttac accaggagtt gcagtggctg
    16801 ttgcgctagg tacgacattt ggtttctttt taacaacgcc ggttatcatc gcattgcgag
    16861 cattgtcaca tcttgtgttt gcgttgattg gggcttggta cttgcaaaag catccgagta
    16921 tcgttttaaa aaatggacag tttacttttg ctaattggcg tttccaaggg ttcaattttg
    16981 tgatcggtgt gattcattct ttagcagaaa tgctagtagt cagcatcttt tactttattg
    17041 gtaatatgcc tgaaacatat tattctcaag gatattttta tacagtattc atcttgatgg
    17101 ggattggtgg gcttatccac agtttagttg attataatat tgcttatttt atagcaggaa
    17161 cactcagcaa atattttgat atccccgtat ttacggcagc aaaaaaagaa aatcggaaag
    17221 agtcgattca agggtacgcg aagaaaacga catattagac atgatctctc aaaaaaaaaa
    17281 ctctcatcta gtaatggata ataaatcctc taagatgaga gttttttgtc ctaaaaagag
    17341 ctgcaccaac atcttctggc acagctctaa cagggggcaa tttatggaag tttgatgact
    17401 tgttctgttt ctttatcaaa gaagtggcct ttgctgatgt tgaaggcaag gtcgactatt
    17461 tcgcctggtt tatggaaatc tcttgcatct acttttgaga tgaactcagt agaaccagtt
    17521 tttgtgtaaa gcatcgtttc agctccaagt aattcggaaa cgacaacttc tgatttcact
    17581 acggcttcag gtgaagcttc aagggctact tgttcactgt ggatgtcttc tggacgaata
    17641 ccgaagataa cttcttttcc ttcataacct ttttcaacta atactttgtt ttttccttca
    17701 ggaatacgaa gcttaagccc atgtccatct gtgataacac catttttcaa tgtcacatta
    17761 aagaagttca tggcaggaga tccaataaag ccggcaacga acatattgac gggtgtatca
    17821 taaacttctt tcggtgtacc gatttgttgg ataaatccgt ctttcatgat aacgattcgg
    17881 tcagccatag tcattgcttc tgtctgatcg tgagtaacat agatggtcgt tgtttcaaga
    17941 cgctggtgaa gtttggcaat ctcagcacgc atggctacac gtaatttagc atctaaattt
    18001 gataatggct catccattaa gaagactttt gcatcccgaa cgattgcccg tcccaatgcc
    18061 acacgttggc gttgtccacc agataaagca gctggttttc gtttcaagta ttctgtcaga
    18121 cctaagatct ctgctgcatt gtccacacgt tttttgatgt ctgctttatc atatttacgt
    18181 aatttcaagc caaaagccat attatcaaaa acagtcatat gaggatacaa tgcgtagttt
    18241 tggaaaacca tggcaatatc acggtcttta ggtgccacgt cattcatgac ttttccgccg
    18301 atatttagtt ctccttcaga gatatcttcc agtcccgcga tcattcgcaa agtagttgac
    18361 tttccacaac cggaaggacc aacaaaaacg ataaattcac ggtctttgat tgatagatta
    18421 aaatcagtta cagaataatt ttctgcattg tcatattttt tatatacgtt tttcagagcc
    18481 atttctacca taatacgttc cacactttct tttttagttt tattaactat ttaaagtata
    18541 aatgaaagcg ttaactaact tcaatgtcat gttgaacaag aattttttct tttttttgtc
    18601 aatgtgctag ttttaagctt taataaatat ccataagaaa taaaaactga tacactggag
    18661 taaataagta tgttaacaat atcaaaagga gtgtctctta tgaaaatcgc tttgatcgct
    18721 catgaccgaa aaaaacctat gatggtgaaa ttagcaacag cgtataaaac aatattggaa
    18781 aaacatgaac tttttgctac cgggacgact gggcagagga tcacggaagc aaccggcttg
    18841 ccgatccatc ggtttaaatc cggaccgtta ggcggggatc agcaaattgg agccatgatt
    18901 tcggaggatc agttagattt ggtgattttt cttcgagacc cgctggctgc acagccccat
    18961 gaaccagatg taacagcact tatccgttta tctgatgtct atgagattcc gttagctacg
    19021 aatatcggaa cagctgaggt gctactaaga ggactggaag ccggcttcct agattttcga
    19081 agaatcgtcc atgaaataga caagcatcca ctttcatttt gatggataag cgtatggaaa
    19141 aataacgttt aatgttcgcc tatatgtagc tataaaagct aaacaaactg ataatatgcg
    19201 atataatacg aacgtattgg ataatataaa caataatatt agtttttcgt atttgcagta
    19261 aatgtgaaaa tatgctatag tttctgtggg aaaagatccc aaaaataata cagagtagaa
    19321 aataaagcgt taagtgctaa gaggatggga tgttgcctct tggacgaagg acgtaatgtt
    19381 cgcggtttta ttttcgcatt cgctgcatga tacgatgtag cagctcttaa aggagatgct
    19441 aaaaatgaag aaacaccggt tagacgcacg tatgattgct attatgggct tattgattgc
    19501 attgatggtc actttgtccc gactggtagc aatcgagacc ccatttatta aaatcagtgt
    19561 cacatttatt ccacaagtga tcatgggcat tctttttggt cctttttggt cagggatagg
    19621 cgcagtttta gctgatttag taggaatggc cctcttctcg aaatctgcat tctttattgg
    19681 atttacattg aatgcattta ttgaaggagc aatctacgga ttctttttct atcgaaaaga
    19741 gatcacttgg aaaaatgcca ttctggctac gcttagtgtc acgctgatca tcaacttatt
    19801 tttgacacca ttatggcttg cgttgatgta ccatgtgcca ttgttcagct gggtcgtttg
    19861 ggcgccccgc ctgttaaaaa cagtaatatg gttaccgatt caatcaattg ccatttatta
    19921 cgtaggacgt agtattcctt acaaaaaaat tttaagaagt ttagcgatcc acgctaaata
    19981 aaaaacaagc agtcttccca tcccagaaga ctgcttgttt ttattgtgct gtgaattgtg
    20041 cttgatagag ccgtcggtaa taaccatctt tttgaagcag ctcatcatgg gtgcccattt
    20101 cgacaatagc tccctgatcc atcactaata tctggtccgc atttttgatt gtggacaggc
    20161 gatgggcaat cacaaaactg gtttttcctt ccatcattgc taagaaggct tgttggattt
    20221 ttttctctgt caatgtatcg actgagctgg tagcttcatc taagatcaac atagggggat
    20281 tactgatcat cgttcgagca atcgtcaaca gttggcgttg tccatctgat atctttaacc
    20341 cttgaccgcc aatcttagta tctaattttt ccggcaaacg ttctacaaat tcatacatat
    20401 aagaggcttt tagggcatca ttgatttctg cgtcacttgc ttccggattc ccaaaacgaa
    20461 gattttctcg caatgtactg tcaaatagcc aagtatcttg taacaccatt ccaaaacttt
    20521 tacgtaatgt atcacgttgg atagagcgga tgtcatggtc atcgactgta attgttcctt
    20581 ttgttgtgtc ataaaaacgc atcagcaaat taacaagtgt ggattttccg gcccctgttt
    20641 ttcctactat agcaatggtt tgacctggat aagcagtaaa attgaaatct tcgatcaaac
    20701 gctgcttggg gtcataagaa aaagaaacat gatcaaaaga gacttttcct ttgatatttt
    20761 tgggttccaa ggcataggca tctggcaaat caggcttttc ttctggctga tcgatcaaat
    20821 caaatgctcg ttctagacca gaaaaagcgg tttgcagttg tgtcgtgatt ccagaaagtt
    20881 cgataaatgg tttagaaaac tgacttgagt agatcgtaaa gctggaaatc acaccaacag
    20941 tgattgacga accactcttc aatgccaata ggccgccaac taaaccaatc gataaataag
    21001 caaggtgatc tacaaaacga gaagacgggt ttgtcaagga agaagaaaat tgtgcccgtt
    21061 gtcctttttc atatagatca gcatttagca gttcaaattg ctgttgacta gcttcttctt
    21121 gctgaaaagc tttcacgatt ttctggttac cgattcgttc ggtcacaaag cccgagatat
    21181 ccccaataat cctttgctgt ctatcgaaat ttgtttgtga agcacgagca acgatccaac
    21241 tcacaaggaa aataattggt gtagaacaaa gaacgaccaa tgtcagaagt ggacttagac
    21301 gcatcataaa aataaacgaa agaatgatga ttgccactcc agaaaataat tgattgaagg
    21361 ccgcagaaat tgcaatggaa atattgtcca tatcatttgt aaatcgactg acgatatttc
    21421 cgtgggagtt ttgatcataa taacgaagtg gcaatcgatt cagatgagca aatgcatctt
    21481 ttcttaattt tgcaacggat aagtaagcta accgattgct taacacttgg atgagccatt
    21541 gactgactac gttgattaaa acgataccac cgaatagaat aagtaaatgg aacagtcgat
    21601 tgaagcggac ctgtccttct ccaatcattt gatcgatact ttgaccaatt tgtaacgtca
    21661 tcacaactgt tgcaatccca ttgatgattc ctagtatcac agctagccaa atgtcttttg
    21721 cataggcagt aaggtaagga atgaatcggc ggagcgtctg caaggataat ttttttactt
    21781 tcataactat tcttcctcct gtgatgcgac gatttcctga tattctgctg attgagcaag
    21841 taattcttca tgggtaccct gagcagcaat cttaccttga tccatcacta agatatgatg
    21901 tgcttcttga atagagcgga cacgttggga gataataatc actgtactgc caagataagt
    21961 ctgtagcgcc ttacgcaaat ccaagtctgt ttgataatcc aacgcactta aagagtcatc
    22021 taaaatcagt aatgctggct tggcaatcaa agctcttgca atcgtcagtc gctgtttttg
    22081 tccgccagag aaatttttcc cattttccat gacttttgta tctaaaccat ttggaagctg
    22141 acggacaaat tcttctgcct gagcgatttt cagagcttcc caacattctt tttctgtggc
    22201 gtctttctta ccccattgaa ggttttcacg aatagtcccg gtaaatagta cagaagtttg
    22261 gggaacaaga gcaatttgct gacggagatg atccagctgc caagaacgaa catccattcc
    22321 gtccattata actgtaccgg aactgacatc atagaaacgc ggaatcaatt gtgtcaaagt
    22381 cgttttcccg cttccggttg gaccaatgat tccaaggact gtatttcgct tcagctgaaa
    22441 tgtaatattt tggagagaaa gaccagaatc aggagtataa cggaagtcaa catggtcaaa
    22501 agcgataatt gcttcagaag aaaaatcggg agtagtactg acctcagctt caagaatcga
    22561 attttctgtg tctagcacct ctttcacccg atttcctgaa gcttcggcac gtgtgaagat
    22621 cacgacgagg tttgaaacaa cgatcagtgc caaaagcatt tgattcatat aattgatcaa
    22681 agcaagaact tgtccttgtt gcaagctacc aatatttact ttgatcccgc caactgtcag
    22741 caaacagatg atcccgacat tcatgatcaa ggttgtagca ggtgataata gggcagagat
    22801 attggaaaca cgtaaataat tgttcgctag atcatttgtg cttttatcga acttctctgt
    22861 ttcttttttt gtctgcgcaa atgcgcggat gacacgaaca ccgctaagat tctgactaac
    22921 gtgttcattg agctgatcta gtttttcttg cactttttgg tagagaggaa cggttttttt
    22981 gatgataaaa aataaaatga ttgagaaaat aggcaataat gctaagaaaa acagacctac
    23041 ttcccagtcg atcacaaaag ccatgaccac tgacccgata cttaaaaacg gtgctctgat
    23101 caataaacga atgaccattg ctaaagctag ctgaagctga ttgatgtcgt ttgtcattcg
    23161 ggtgatcaag gtatctgttc caaaatgatt caactcgttg tgagagaaag tattgatttt
    23221 tttgattagc tgattacgta actctgtacc aaatccttgt gaagctactg aagcatagta
    23281 ttggcaaatg atagcacaaa tcagaccaat cacagacata gcgaccatcc ataagcccat
    23341 ttcaatgatt tttcctcggt cattcatttt tagtccatta tcgatcaagg aagccatcat
    23401 taaaggcagg acaagttcga agacagcttc aagaaatttg aagaacgggc ccaaaatgat
    23461 ttgcttacga tagtcttttg cataacgtaa taactgaatc atatgattcc tcctattatt
    23521 tatttgtcca caattgaaaa aacaagtact accacattgt agcttaggaa atttagaatg
    23581 aaaagagaac aagccttttc ataaatgaaa gttttattat gaagaagctt tagaaattga
    23641 atagactatt ttttttaaaa aaaagaaaat gagaaaacat gccagtctct tcattcatag
    23701 gaacgtctat tttacttaat ttttctataa atgaattctg cataaaaaat atatcaagta
    23761 aatgtttata gatgaaactc tatttacttc ttttttaatt tgttcttgca atcttttttg
    23821 ctacgtaatt tttgtaaaaa agtgggttga gagaaatcta attgattttc aaagaaggag
    23881 tatgaaaatg agaaaaatat ttattcgttt attgtgggta ctatcttaga ggaataaata
    23941 atagtggaga aaactatggt ttggcgcaga ttctatcagg tttgatggaa cgaagacaac
    24001 ctcccgacat caagtcaaaa gctacttgag attttgatct gaaaataaaa aaagaaaaca
    24061 aggtaggaac atcaatgttt ctaccttgtt ttcgtaatca atgggaaaaa actaagagtg
    24121 gtgtacgtaa tcaataaaaa ggatggtgac aacagtacct ctttactctc tgaaagcatc
    24181 ataactcgtt tttcctcatt atgccaataa attgttgtta taccacatat aaggatttgt
    24241 aatagctaaa aacagacagg aaagttgaaa tctgtgaatc ttattttgta tggaaggatt
    24301 tatagaacgc aaaaatcaag ataaagatga ataaaaatcc tgtttcggct atatattgcg
    24361 tatctacgcc aataaaacga gtttgttctg tcatagccat ataaagcaaa ggcaaaaata
    24421 caactacagc gagataagat ccgatgattt ctcgaaaagt tttccaggaa gtatgtttcc
    24481 tgtttttata tttatccgat ttcattgaat agtcatagcc tccttattct ttcatatgtt
    24541 gaaattccag tataaaaact ggaaggaact gtacgttttt tagaataaaa attataaggg
    24601 ataagtaggc tgctgtctat aataagatat atttttcaat tctttattaa agattttatt
    24661 gacaaaatga atccgttttc tatgatggag gaggggtgaa taagttgttg aagaaggaaa
    24721 ttttatgtta cttagagaac cagacagcct ttttcgattt agaacatatg aacgaggtct
    24781 ttacagcaca aaggttagct gatcattttg gggtaaaaag aaacacagtc agtcattatt
    24841 tgaaccagtt aaatgatgag caactactag tcaaaatcaa ttctcgacca gtgatttatt
    24901 tccacaaaga agcttttgaa aaacaatttt ttaagttacg cacgaatttt tatttttcta
    24961 tcgatgatct caaagaggaa cagccttttt tcgcgcaacc aaaagactta ttctctttta
    25021 tgatcggata cgacgccagt ttgaaagaaa gtatcgaaca aatcaaaaca gctctttact
    25081 atccagatgg cggattacca cttttgatta caggcgaaag tggcacaggt aaaagttatt
    25141 tagttaattt ggtctatcag tattgtctgc ttcatgatct gctagaagat tctgcaccat
    25201 tcatcacttt taattgtgca caatatgcag ataatcctga acttttgact agcaatttgt
    25261 ttggacatgt taagggagcg tacactggtg cagaagaaaa taaaaaaggg gcttttgaag
    25321 cagcagatgg tgggatcttg tttttagacg aggtacatcg cttaacacca gaaggtcagg
    25381 aaaaactgtt cacctatctt gatcaaggtg tgatttatcg tatgggagaa cctaatttgt
    25441 cacgccggat caaaacacgt ctcttttttg ctacgactga agagattact agtcatttct
    25501 taacaacttt cattcgaaga atacctatcc gaatcgaact acctgcactt aatcagcgta
    25561 gtcgtaatga acggctagaa ctgatttact ctttttttct cgaagaacaa agaaaaatgg
    25621 ggagcccttt gtcagtcagt ggaccagctt tgtcactttt aacaagtcag aaattatccg
    25681 gaaacatagg agagctgaaa aatatcgtca aagtttcagc agcaaaagca tatgctgaac
    25741 agcgtgaaca gcctgaaatc catgtcacga tccatcattt gcaaaaagaa ctacttgcgc
    25801 agcctattac taaaaataca gcacagcaag gaatctatat tacgcaagac cagaccttag
    25861 agcaattaat ggagaagcaa cagcccgaac agcagcgaat cgttaaaagt tttgagcaaa
    25921 tcctaattga tttcaagaaa aataactgtc tgctatattc ttgcgaagga aaattaaagc
    25981 aagaagtgat tcaattattt gattttttgc tgtttgagac cagtagacaa gaaaaacacg
    26041 agctgctggt ttattggaca caatctatcc gagagacctt tcgacaaatg gaaactgcct
    26101 atcaaatcaa attcgatgga aacagtgtct atgccttgag ttactatctc tatcagcgaa
    26161 gcactatcaa atggttaccg gaagaacatg atattatcca actaatcaaa gaattagaac
    26221 aacaagtttc tcagtcttat ccatctagtt atcactatgt ccaacgaatt ctggaattaa
    26281 gtaagatcaa attagatatt gaagacacaa gtatggatcg gattttactg acgatctatc
    26341 taaaaaaagc gaaatggtca aaagaaaaac gactgccaaa agcgatgatt gctgctcacg
    26401 gttatgcaac tgctagcagt atggcaaatg tagtaaatcg tttattacaa gaggatctat
    26461 ttgaatcatt tgatatgcca ctagatgtca caccacagca gattgcttca gaaatccttg
    26521 attattgcga aaacagcgat gtttcaaatg gattgatcat attagtagac atgggatctc
    26581 tgaaagaaat ccatcagttt tttaaaaaac agttatccgt accgcttttg attttgaata
    26641 atgtgacgac acctttagca atcacagtag gggaatgcct gcaaaaaaat gtatcattag
    26701 aagagatagc agaagagact gttcgacaga tccagccaga atggcgactg ctctatccag
    26761 aggaaaacaa acccaaagca ctcattacta cttgcttcac tggaatcggt acggcggtcc
    26821 acttaagtga actgctagaa aaaagcttac ctacggcttg tcagttgaaa atcatcccat
    26881 atgaatatca gcaattaaaa gataagaaaa acagtgaccc attattttcc atatacgaag
    26941 tcgtaggaat gattggcacc acagatcctg atatcaccaa tatcgactat ctttcattag
    27001 aagacctcat ctcgggggaa aagatgtctg tactagctga atggttggac actactatga
    27061 atgcttcaga gaaggaaata ttcaatcagc gcatcattcg taatttttca ttggaaaaag
    27121 tactggattc cgtgactatt ctagatacag agaaggtgat gggagaaatc gatcatttta
    27181 tgagagaatt agaggtgcag ttgaatcgaa gcatcagcaa tcccaaaaaa ttagcgttgt
    27241 atgtccatgt gagctgtctc attgaacgat tgatccgaca aatgccaatc gaaacctatc
    27301 aaggattaga gaaatggaag cagtgtcaaa aagaagggtt atctgctatt aaatctgcgt
    27361 ttagtgtcat tgaagagcat tatagtgtca tgatccccaa ttctgagatt gcctatatct
    27421 atgatatttt gagtgaagat acagagtttc tctcgataga agaagaattt tgaacataag
    27481 caagttgttg ccactaattt tggcatgcaa cttgcttttt tagtatgtgt aagtgaaaag
    27541 aggagatgaa aaagtggata gagaaattat cttagcttcc catggaaaat ttgcttcagg
    27601 gatattagat tcattagagc tgatctatgg gaaaaatcat tctattactg tattcgattg
    27661 ctatactgat gagaattttg atttagcgga aacagtaagg cagcttttta tgaggtacaa
    27721 agataaagaa atcattgttc tgacggatct ttttggcgga agtgtcaaca acgaattctt
    27781 acaatatatc aatcgggatc atgtctatct cgttgccgga ttgaatttgc cactattgat
    27841 tgagctagta tctcggttgg acgctgaagt agagacaact gttttgatcc agcaggtgct
    27901 aactgattca aaaaaaatga tccagttttg taacgagcgg ctcaatcaga aaatagaaga
    27961 agacgaattt taggaggaag aaaaatgatt gtattgacaa gagtcgacca tcggttatta
    28021 cacggacaag tagctttttc atggacacaa acaataggcg cagactgtat tttgattgct
    28081 aatgatgatg tacctacgaa tgagatacga aagacaacga tcaaattagc caagccacaa
    28141 ggagtcaaac tagtgatcaa atcaattgat gactcgattg ccgcgttgaa aagcggggtg
    28201 acagataaat ataagttatt catcgtagtg gaatcgatcg aggatgcata taagctggca
    28261 gaagcgtatc cagaaatcaa aaccatcaac ttaggaggga tcaaggcaaa acctggtact
    28321 cgcagcatcg gaaaagcggt aaatatttta ccagaagagg aggaacgctt gaaacgatta
    28381 attgacaaac aaatagaagt agaaatccgt caagtaccag cagataaaaa agtggatgta
    28441 agagaagttt tgtaagaaag gaagaacctc aatgattgta caagcagtct tattaggaat
    28501 tgttgcattt gtcgcacaat cagaatatgc actagggaca tcgttgcttt ctcgtccgat
    28561 tgtgacgggg ctgtttacag gaatcgtgtt aggtgatgta aaaactggga tcattatggg
    28621 cgcaacttta gagttagcat ttatcggatc gttctcagta ggtgcttcga ttccacctga
    28681 tgttgtgacc ggtgggattt tagggacggc ttttgctatt acagctggag ccggtacaga
    28741 aaccgcgctt ttactaggtt tgccgattgc tacgttgaca ttgatattga aaaatattta
    28801 cttagggctt ttgattccaa ttatgaacca taaagcagac aattatgcag aagaaggaaa
    28861 ttataaagga gtcgagcata tgcacttgct tgccggtatc ggtctttcct tgatgcttgg
    28921 tgttgtggta atggtttctt atatggtggg aagcaatacg atcggaaatc tattaaatat
    28981 gattcctgac ttcgttcaaa aggggttgtc agtagctaca gggttgattc cagctcttgg
    29041 ttttgcgatg cttgctcgtt tgctgatcaa caaacaagtt gcaccatatt ttttcttagg
    29101 ttttgctata gcttcttact tagagattcc tgtgaccgga gtagctattt ttggtgcgat
    29161 tttagcagtt gtggtagtca atattatgaa tgtccgtcaa ctaccacttc aaacaactgc
    29221 aggggaggta gaagaagatg aagaattctg agatcaaaaa cagcaaaata acgaaaaaag
    29281 agctaaatca cgtattttgg cgttcttttc aaatggagtt ttcttggaat tatgaacgtc
    29341 agatgaatat ggcttatgtt tatgcaatga ttccgatttt gaaaaagtta tatcagtcga
    29401 aagaagaaat ggcagcagct ttgaaacggc atctagagtt ttttaacacg accccacata
    29461 tcgttacact gattttagga atcacggctg caatggagga agaaaataag gatgatcctg
    29521 aatttgatgt gactgcgatc gatagtatca aaacatcttt gatgggacct cttgcagggt
    29581 taggagattc tttcttttgg ggaactttgc gattgattgc cacaggtgtg ggtacctctt
    29641 tagccatgca aggaaatatt ttaggcccta tcttgttcat tttgattttc aatattccgc
    29701 acattctgtt tcgctatcta gcaacaggtt ggggatatcg cttaggaacg ggttttttga
    29761 aaaagatcca ggcaaacggt atgatggaaa gtttgacatt aggcgcgtct attattggac
    29821 tgatggtcgt tggagcaatg actgccacga tgatcaccat caatattcct ttaaaaatcg
    29881 gttcaggaga aaatgctgtg actgttcaaa gtatttttga tgatatcgtt ccaaatatct
    29941 tatgtcttgg agcatttggc atagtgtttt atctattgaa gaaagaagtg aaaccattaa
    30001 cgatcttgat cggactagct ttctttggaa tttttggttc gtggataggg attttttaaa
    30061 aaaggagcag agaaaatgtt tacgatgcaa gattatatct atgaagaaaa ggaagtacta
    30121 agtacgatct tgaaaaagaa tgatttttcg acaagagaac atatgaaaaa aacagtgaat
    30181 ttactgatct tagcaacggg ttcatcttat aacgcttgtt tagcagcgaa gccagcattg
    30241 gaatcttatg gtgatcttac tgtcgatatt caagaacctt tttatttttt caattatgga
    30301 aaactttctc cttcaatcga tactgtgatt gctgtgtcac aaagcgggaa aagtgcttct
    30361 actgttgaag cagtaaaaat gatacaaaaa caagggttac ctgttgttgc tatcacaaat
    30421 gacgtgcaaa gtccacttgc tttagaagct gcacaaatta ttgatttagg tgccggggta
    30481 gaatcagtag gcttcgttac aaaaggctat tctgcgactg ttttacagtt actactctta
    30541 gggataggaa ttggaataag taaaaataaa atcagcaaaa aaatagaaca agactacatg
    30601 caacagttgc gcaaaataat caaccattta ccagcgatta ttcaaaaaac ggaagaattt
    30661 tttgatggat atcaaagttt atttcgttta gcacagcgtt tcgtcaccat tggatatggt
    30721 ccaaactggg gaacagctaa agaagcagag acgaaattga ctgaaacaat ccgcgttccg
    30781 tcacaaggat ttgagttaga agcttatatg cacggcccat acctggaggc agatgcttcg
    30841 catttgctat tctttattga aggggaaagt gtaaataagg aacgttcgca aaagttgcaa
    30901 agatatatga gtcggtacgt tggagagact ctcaccatta ccacaaaaaa agcaagaaac
    30961 gagaaaacac ttggattagc aatagaatgt gatgaatatc tgtctgtatt agcgcttgtc
    31021 gttccgtttc aactattcgc ttataaaacc gcagtagcta aaggtattga cttgaacaaa
    31081 aagatttttg aagactttga tacagtcttg aaaagtaaat tataagaggt ggaaatcatg
    31141 ctgaaattca atgaagaaga acaaatcaaa gcaataaaag gcgcactagc tttacgtccg
    31201 caagtagaag aaatcatcga taaactttat gcggaaaaat ttgacgctgt ctattatcta
    31261 ggaatcggtg gaacgtatgc ttcctcgatg caagctgtca cttatatgaa tgggaaaagc
    31321 aacttgcctg tctttgtaca gcatgctgca gaatattaca ctactggaaa caaaagatta
    31381 acaaaagatt ctatcgttat tttgtcctct gttaccggta caactcaaga agtagtgaaa
    31441 gcagtagaac aaatcaagaa agtaggagcg acactgattg gatttatcga taaagcgaac
    31501 agtaagttgt ctcagctatg cgattttgtg gtcacttatc cagctcctgg aacagaacaa
    31561 atcaaatttt ttatggcagc agatcgattg atgtatttga atggagaatt cgaagcttat
    31621 tcagaatact atgaacagct cgaacgttat ttaccaacag ggttagtgga agcagagaaa
    31681 aaagcagatg catttggctt atcatttgca gaaaaacatc gacgtgatag tatgcattat
    31741 tttattggag cagggaatca atggggagct gtttattcat atgccatgtg ttactgggaa
    31801 gaacaaagct ggcttccatc gaaatctatc catgctgccg agtttcttca cgggacgtta
    31861 gagatcgtag aagaaacaac cccagtcaca ttatttttag gcgaagatga acagagagtg
    31921 ttatctgaac gtgtggctaa acttttgcca aaaatttgca gcaattatac attgatagac
    31981 acaaaagact atccagtaga aggtatcagt gaaaaatatc gcggacgtgt tttgtctttc
    32041 ttattgatgc atgtagtgac acaacgcatc gatgctcatg tggagcagtt gaactgccat
    32101 ccattagaga tacgccgcta ctatcgtcag tttgattatt aggatagact ggataaaaag
    32161 aatataaaaa ataaagagag cagcgataac tgctcccttt atttttttaa aatattggca
    32221 tcaattacgt tataaaacac attttttgca ttatctacag ttcaacttat aatctgttta
    32281 atgggacatg tgggtgccaa ccattttttg ttatgcaata gtactattta gttgttgcat
    32341 ggatccgtag taaaagtcca agtacaacga gttgcgccag tttacatagc gttttgtatc
    32401 cagaatgttg actaaaacaa aagttcctat gtcaaccgtc aacagagaca atctgtccgt
    32461 tgcaggacct gacagattta gaaatgttca atctgtctct tatgtatttt aaatcaatac
    32521 tcactaggag aagtttccta acctcctaat agtaatgctg gtagtaacat tgtcaaaact
    32581 gcttcctctt aggaacaagc aaagcataac gagtgtaaaa ataaaatgta gactattgta
    32641 acatatcatt gattatttca agtaaaaacg ataaaagaag taaacaaaca cttaaaaaca
    32701 atggaaatta gtcccaactt taagaaatgt atactataat ggaaattatc aattatttat
    32761 ttggaggaat atccatgaaa caaaaaatcg cagttcttgg tcctggatct tggggcactg
    32821 ctttagcaca aacattggca gaaaacgggc atgatgtccg tatttgggga aatgtacctg
    32881 aacagattga tgaaatcaat aagtatcata caaatcagca ttttttacct gatttgacga
    32941 tcccagaatc gattatcgga tataaagaat tggcagaagc agtggataac gcagatgcta
    33001 ttttatttgt tgttccaaca aaagcgattc gttcagtcgc acaggaactt attggtaaaa
    33061 tgaatactaa gccaatcatt atacatgcaa gtaaaggact agaacaagat acgcataaac
    33121 gaatctcaga agttttggct gaagaaattc cagaagaaaa aagacaggca attgttgtat
    33181 tatctggtcc tagccatgct gaggaagtgg cagtacatga tatcacaaca attacagctg
    33241 caagtattga tcaaaaagca gcagcctatg tacaaaaact gttcatgaat gattatttca
    33301 ggatttatac aaataatgat gtgattggcg tagaaactgg tgcagcactg aaaaatatca
    33361 ttgcaattgg tgccggcgct atccatggat taggattcgg agatgatgca aaagcagcaa
    33421 tcatgactcg aggattggca gaaatcagcc gtcttggtgt cgcgatgggt gccaatcctc
    33481 tgacatttat cggtttgagt ggtgtggggg acttgatcgt tacttgtaca agtgttcatt
    33541 ctcgcaattg gcgtgccggt aacttgttag gacagggaca taagctggaa gaagttctag
    33601 aaaacatggg aatggtcgtt gagggtgtgg caacgacaaa agcagcagta gagctagctc
    33661 aacagctagg tgttgagatg ccaattactc aaacaatcta taatgttttg tataatggag
    33721 aagatatcaa aaaagcagca aaagaaatca tgttacgtga cggaaaaatg gagaatgaat
    33781 tttctcttcg ttaaatagat gatcaatagt taatcgt
//
LOCUS       DACGLW010000024        31867 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.024, whole genome shotgun sequence.
ACCESSION   DACGLW010000024 DACGLW010000000
VERSION     DACGLW010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31867
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.024"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            complement(136..2289)
                     /locus_tag="IXT53_001158"
     CDS             complement(136..2289)
                     /locus_tag="IXT53_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HAQ4904999.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFAPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKALELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYTPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTAAKPLASAKMDLSAKTGTAETVAEGHPDITTVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHMNLTIAKEIMDAYYDMFMAK"
     gene            complement(2583..3431)
                     /locus_tag="IXT53_001159"
     CDS             complement(2583..3431)
                     /locus_tag="IXT53_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ4905000.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(3655..5595)
                     /gene="thrS"
                     /locus_tag="IXT53_001160"
     CDS             complement(3655..5595)
                     /gene="thrS"
                     /locus_tag="IXT53_001160"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HAQ4905001.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(5932..6111)
                     /locus_tag="IXT53_001161"
     CDS             complement(5932..6111)
                     /locus_tag="IXT53_001161"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HAQ4905002.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(6158..7981)
                     /gene="cadA"
                     /locus_tag="IXT53_001162"
     CDS             complement(6158..7981)
                     /gene="cadA"
                     /locus_tag="IXT53_001162"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAQ4905003.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            8259..10538
                     /locus_tag="IXT53_001163"
     CDS             8259..10538
                     /locus_tag="IXT53_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ4905004.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(10631..11278)
                     /locus_tag="IXT53_001164"
     CDS             complement(10631..11278)
                     /locus_tag="IXT53_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905005.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(11457..12176)
                     /locus_tag="IXT53_001165"
     CDS             complement(11457..12176)
                     /locus_tag="IXT53_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HAQ4905006.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(12307..13458)
                     /locus_tag="IXT53_001166"
     CDS             complement(12307..13458)
                     /locus_tag="IXT53_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HAQ4905007.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(13675..14415)
                     /locus_tag="IXT53_001167"
     CDS             complement(13675..14415)
                     /locus_tag="IXT53_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ4905008.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(14419..14742)
                     /gene="rsfS"
                     /locus_tag="IXT53_001168"
     CDS             complement(14419..14742)
                     /gene="rsfS"
                     /locus_tag="IXT53_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HAQ4905009.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(14799..15392)
                     /locus_tag="IXT53_001169"
     CDS             complement(14799..15392)
                     /locus_tag="IXT53_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ4905010.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(15376..16023)
                     /locus_tag="IXT53_001170"
     CDS             complement(15376..16023)
                     /locus_tag="IXT53_001170"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HAQ4905011.1"
                     /translation="MNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHLV
                     MAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIELA
                     RGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPGY
                     TTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(16036..16347)
                     /gene="yhbY"
                     /locus_tag="IXT53_001171"
     CDS             complement(16036..16347)
                     /gene="yhbY"
                     /locus_tag="IXT53_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HAQ4905012.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(16363..17472)
                     /gene="yqeH"
                     /locus_tag="IXT53_001172"
     CDS             complement(16363..17472)
                     /gene="yqeH"
                     /locus_tag="IXT53_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HAQ4905013.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNEIQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(17469..17996)
                     /locus_tag="IXT53_001173"
     CDS             complement(17469..17996)
                     /locus_tag="IXT53_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HAQ4905014.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(18176..18961)
                     /locus_tag="IXT53_001174"
     CDS             complement(18176..18961)
                     /locus_tag="IXT53_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HAQ4905015.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(18954..19823)
                     /locus_tag="IXT53_001175"
     CDS             complement(18954..19823)
                     /locus_tag="IXT53_001175"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HAQ4905016.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(19824..21203)
                     /locus_tag="IXT53_001176"
     CDS             complement(19824..21203)
                     /locus_tag="IXT53_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HAQ4905017.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAKQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(21211..21630)
                     /gene="fabZ"
                     /locus_tag="IXT53_001177"
     CDS             complement(21211..21630)
                     /gene="fabZ"
                     /locus_tag="IXT53_001177"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAQ4905018.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(21655..22131)
                     /gene="accB"
                     /locus_tag="IXT53_001178"
     CDS             complement(21655..22131)
                     /gene="accB"
                     /locus_tag="IXT53_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HAQ4905019.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(22134..23369)
                     /gene="fabF"
                     /locus_tag="IXT53_001179"
     CDS             complement(22134..23369)
                     /gene="fabF"
                     /locus_tag="IXT53_001179"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HAQ4905020.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(23388..24125)
                     /gene="fabG"
                     /locus_tag="IXT53_001180"
     CDS             complement(23388..24125)
                     /gene="fabG"
                     /locus_tag="IXT53_001180"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HAQ4905021.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(24137..25054)
                     /gene="fabD"
                     /locus_tag="IXT53_001181"
     CDS             complement(24137..25054)
                     /gene="fabD"
                     /locus_tag="IXT53_001181"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HAQ4905022.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(25161..25385)
                     /locus_tag="IXT53_001182"
     CDS             complement(25161..25385)
                     /locus_tag="IXT53_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HAQ4905023.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(25438..26400)
                     /locus_tag="IXT53_001183"
     CDS             complement(25438..26400)
                     /locus_tag="IXT53_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HAQ4905024.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(26410..26850)
                     /locus_tag="IXT53_001184"
     CDS             complement(26410..26850)
                     /locus_tag="IXT53_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ4905025.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            27156..28121
                     /locus_tag="IXT53_001185"
     CDS             27156..28121
                     /locus_tag="IXT53_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HAQ4905026.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(28178..28408)
                     /locus_tag="IXT53_001186"
     CDS             complement(28178..28408)
                     /locus_tag="IXT53_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HAQ4905027.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(28494..29381)
                     /locus_tag="IXT53_001187"
     CDS             complement(28494..29381)
                     /locus_tag="IXT53_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HAQ4905028.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(29470..30693)
                     /locus_tag="IXT53_001188"
     CDS             complement(29470..30693)
                     /locus_tag="IXT53_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ4905029.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(30710..31789)
                     /locus_tag="IXT53_001189"
     CDS             complement(30710..31789)
                     /locus_tag="IXT53_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HAQ4905030.1"
                     /translation="MLYYISEGGRKVKVLLYFESEKMLAKSGIGRALDHQKRALTEVG
                     ISYTLDEKEDYDILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQL
                     SPIVKKYLVGLYQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQ
                     KFREYFKLSPDQKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIR
                     KIVKEDHPENVLFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVR
                     DIPVYNGWLEDSKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEE
                     LKNVYESVLNEKVSSKLKKVNQVKD"
BASE COUNT         8807 a         6974 c         5489 g        10597 t
ORIGIN      
        1 cttttttgcc gagtaaacgg tgttcaaaag ccagtttctc ggtatgaagt caaatttaag
       61 caaaaatgtg agaagctatt ttcacaaaat ttttcttcat actcgaaact aagcggcttt
      121 ttcacgacct cttttttatt ttgccatgaa catatcataa tacgcgtcca ttatttcttt
      181 agcaatcgtc aagttcatgt ggtcttgttc gtctaataag ttcggcaaga caacactgat
      241 ggcaatttcc ggattgtctg ttggtccgta tgccacgata ttactattga ctgtcgtaat
      301 atctggatga ccttccgcaa ccgtttctgc agtccctgtt tttgcggaaa ggtccatttt
      361 cgcactggct aatggtttgg ctgctgtgta aggatcggta ccatggacaa cttggtggaa
      421 tccttcacgg aggatcgcca tattttctgc agaaatattg accgtatttt ctactgttgt
      481 gccaatttct ttcttcactt cacccaagtt tccattttcg tcattgccat agatcgcttt
      541 gactaggtgc ggctgtactc gtttcccgcc attagcgact gttgaagcat attgagctaa
      601 ttggattggt gtatatgtgt caaactgtcc aaatgccaaa tcggtaaagt tacctggagt
      661 ataccattct ttgccattat ccgcattgaa cttcttcata taatcaacag aacgtgagat
      721 cccaggagat tcattcggca aatcgatccc agtcgttgta ccaagaccaa actctttgaa
      781 agagtctctc aattgctgat aagctttagc ttcttcatct aaactaggta gtcccattcc
      841 aggtgtatag tctagtccta acatctttaa cgccactttg atcatatacg tattagaaga
      901 caattccaat gctttgactg catcaagagc aacttgtccg cttcgattaa agacagagct
      961 tttttctgaa gcaccttgca gacgaatcgg ctcatcaatc aaaacttgat ttccgctgat
     1021 cgcgttactt gcccagcctg ctgtcaaagt accagccttg actacagacc ctggagcaaa
     1081 ggcgctagta atcgtgccta aagcattctc ggtgatttct ttggaacctt gttcatgact
     1141 gaaaccagtc attgctagca cttctcctgt ctgaggattc atagccactg cataagcacc
     1201 aggcgaatac tgtgctttgc cgttgttgat caatgtttga taattccttt tcaaaatttc
     1261 ttctactttt gattggaatt ctgcattcat agataacatt aaattagaac ctttttcccc
     1321 agagaagatt tctttttgat tcgaaacatt tccttcatta tcaagggaaa cttcatattg
     1381 ggttttggtt ccttgcagga catcttcata ttgtttttcc aaataacttt gtcctacacg
     1441 gtcattccga gaataacctt ttgctaggta tttctctgct tcatctgctg gaagtccttg
     1501 cttttcagtc gtcacactgc caagaatact tttcagtgaa cttgctgcat tatattctcg
     1561 tgtccagtct gtgccagtcg aaactcctgg aagttcagaa gcgcgttctg ccacaacagc
     1621 taactcttga tcagtaacac cagagttttt gatatagaca gtgtttaatt catatgcact
     1681 attcatccgt ttaaagatgg tagcaatttt taattgctcc tcgtttaaat taatctcatc
     1741 agctgtgact ttattgacca tcgcactata ttgttcgctg ttccctaatt gcttttcttt
     1801 tgccgaaagt ctttttgtgg cttctttcaa atgtttggga tcagccagcc aaaaatcttt
     1861 tttatctcta tctgtcagat tttcatctac cggaacatcg atcagcgagt tcagcttcgt
     1921 tgcgatttca agcaaatctt tagccgtaac ttgtgttcct ctcgtatatg tgatggcctg
     1981 attcgcttta ttcgtaacta aagcttttcc tgtggagtcg tagatcattc cgcgaggagt
     2041 actttcttgg accgtaatcg tagaagtaga cttcactcgc gcctccatct cttcgccgtt
     2101 cacgatctgc aaataaccca atctagcaat caacgcaaca aataacgtaa atataatgaa
     2161 aaaaagaaaa tttaagcgaa aaggaacatg ggaccggcga aacgttttgt tcttaggttc
     2221 ttgtgcacct tttcttattt tttctaacca agaagtattt aatatttttt tcataaaatc
     2281 tttcatcatt tcctatcctt ccgcctattt acattcatgc ttcattgtac ccaaaaactt
     2341 cttaataaaa aagaaaaaat gggcggagca agaaattctt tacctaattt caagataaaa
     2401 tcaacttttt gtttcacagc aatcacttaa agttacatat ccaactattt ttttcattaa
     2461 aaaataaact tatttttgaa aattatgttt tcttaagtcg tgaaacactt tgatggatca
     2521 tttatttgtg gaaaactacg ttttgttatt ctaaaaaact tatataaaaa atagtaaggt
     2581 ttttatttcg aatccataaa atattcttgg attgcttctc gtgttttttt cactttttcg
     2641 ctaagctttt cgtttttagc catatagaaa taaaaaatga tatcgatcgt agcaaattgg
     2701 gccaaaagtg aattagttgc cgcacttcga ttcgtgattt cttttggtct agatgtttgt
     2761 acgagtacat cggctatctt gctaagcggg ttatttccaa attgcgtcaa cgcaatgatt
     2821 tcgatattca tgcttttcgc cagctcggct aatgcgacta catctgcact tctacctgag
     2881 tttgaaacaa gccaaagcac tttctttttc tcattgctga caagttgcgg gagcaagacg
     2941 tggatatctt tttcaaaaat gattggctta ccgacacgag accatttttg cagaatatct
     3001 tctactacta aagaagaggc gccaacccca tataaaaaaa cagtctctgc cgattccaaa
     3061 cactctacga ctttttggat cgtttcttct tctagaatat ctactgtttc ttttatcgct
     3121 acttgtgcat tgttaccaag tttgtttttg atagagtaaa aggactcatt tgcttctata
     3181 tcactaaaac cttcatcgat tccagtctgt agttctgttg aaatcgtgat tttgaagtcc
     3241 gtcagacttt gaaaaccgat ttttttggaa aaacgtacaa ctgtcggcgc actcaacccg
     3301 gaagcttctg ctaattcatt agctgtcatt gtagggacct tctcaatatt accgataatg
     3361 tagttggcca actttttttc tgctgaagaa taatcttcca tcatactttc aatacgacta
     3421 attacgctca taagttcctc caccctttta atgatagtct atgtaagtat accatatcag
     3481 acccttattc acggtcgtat actggataaa aaacaaaaaa acaatccgcc caacagaata
     3541 tcttatccca tcaatcccac agattctatt gagcatctgt gtctttgagg aatacctgtt
     3601 atctattgtt cggattgctt tctatctact acttttttac aggaaattta cctattagtt
     3661 ttctctgctg taatttttga cttctgcttc catagcatca ataaagatat tcaaatcttc
     3721 tacagctgtt tctttacttc cataacgacg tacgttcact gttgcatctt ccaattcctt
     3781 gtctccaaca actaattggt aagggatctt ttgtgtttga gaggcacgta tcttatagcc
     3841 cattttttca ttacgatcat caacttctac acgcaacccc ttcatttgca aacgttcttt
     3901 gatttcataa gcataatcgc catgtgcatc aacagaaaca gggatgattg ttgcttgtat
     3961 aggtgccaac catgttggaa aagcgccttt gtatacttct gttaaataag cgacaaaacg
     4021 ttccattgta gaaacaattc cgcggtggat aactactgga cgatgcgtat tttccccgtc
     4081 ttctccaaca tatgtcaaat caaagcgctc tggcaataag aagtctaatt gtatagttga
     4141 taatgtttct tctgtaccaa gagctgtttt gacttgaaca tctagttttg gtccgtaaaa
     4201 ggctgcctcg ccttctgctt caaagtaatc tagttctaat tcatccatag cggctttcag
     4261 cattgcttgg gctttttccc acattgcgtc atcatcaaaa tatttatctg tattgtttgg
     4321 atcgcgatag ctcaagcgga aacgataatc tgtaatattg aagtcagcat atactgccac
     4381 catcaattca agcgtacgtt taaattcatc tttgatttga tcaggacgaa caaatgtatg
     4441 accatcattc aacgtcattt ctcgtacacg ttgtaaacct gacaaagcac ctgatttttc
     4501 atagcgatgc atcattccta attcagcgat acgaattggt aattcacgat aagaatggat
     4561 cgtgtttttg tagaccatca tgtgatgagg gcagttcatt gggcgaagaa caagcatttc
     4621 gccatctccc atatccattg gcgggaacat gtcttcttga taatgatccc agtgtccaga
     4681 tgttttgtac aactcaacat ctcccattat tggtgtatat acatgttggt aacctagact
     4741 aacttctttg tctacgatat aacgttcgat cgtgcgtcgg attgttgccc cttttggaag
     4801 ccagaatggc aaaccagaac ctacttctgg ggaaaccatg aacaagtcaa gttcttttcc
     4861 tagtttacga tggtctcttt cttttgcttc ttcacggaga cggatatatt ctttcaaagc
     4921 tttcttgtca aaaaaggctg tcccgtatac acgttgcatc atttgattgt ttgagtttcc
     4981 acgccaataa gcacctgcca ctgataataa tttaaatact tgtatacggc cagtggatgg
     5041 aacatgtggt ccacgacaca aatcgatgaa ttcgccttgt tgataagcag taatcacttc
     5101 ttcttctggc aattcactaa tcagttctac tttgtacgga tcacttgcaa aaatttctaa
     5161 tgcttcctgt tttgaaagaa cacggcgttc gatcggcaga ttttctttga cgattttcat
     5221 catttctgct tcgattgccg gcaaatcttc tgcagttact tggttttgtc cattatctgt
     5281 atcgtagtaa aaccctgaat cgatagctgg tccaacgcca aagtggatat ttggatagag
     5341 gcgacgcatg gcttgggcca tcaaatgagc tgctgaatga cggactaagc caagtgcatc
     5401 ttcatggtct ggtgtgatga tctcaatact tgcatcttcc tcgattggac gagtcaaatc
     5461 aacgagtttt ccgttcactt taccagctaa agcttttttt gctaagcttt ttgaaatgct
     5521 ttcagcaatt gccaatgttg ttgttccaga ctcaaattct ttgactgcac catctggaaa
     5581 agtaattttt atcataatag tttcctcctt gaaatgtttt gattgatcag atcatcaagc
     5641 tgaaaaacaa aaaaatccta gcatctcttg aaaagagata ctaggacgaa aagttcgtgg
     5701 ttccacctaa tgttcaaacg caaatgcgtt cctcaagcgt tgtaacggtc cgaccgccct
     5761 tgcttcaaca agggtttcga aagtggtcat cctttggttt tctcctggaa aagtttcagc
     5821 ctagcttttc ctctcttatc gaagactctc aaattcagtt ggctttctca tcaatcaatt
     5881 atgtgtttat ttaaccacta ataaatagaa aaatcaagtg ggaaactaaa cttatttttt
     5941 ccattcccca tttacaccgt atgttccttt tttcatctca ttgatgatga catggatgtg
     6001 ttcttttggt gcctgcgtgt ttttatgaac agcttccgtg atatctttca tcatattttc
     6061 taattgttcc ggactgcgac cttctactaa ttcgacatgt acgaatggca taatgctctc
     6121 tcctctttct gatcgttctt ttttcaactc gattctatca tactatgttt gtcttgggta
     6181 ctgaatcagg aaaaatggct ggatcttttt ttgctctcaa ggcactcaaa atagagaccg
     6241 tatccacgac ttcttgcaaa acagctccga ataatgcggg gattagtccc gtacttgcaa
     6301 ttaacatcaa aaaaatacaa ataaagatgc caatcagtac agactgtttc gctattctca
     6361 tcgtatctct tgaaatcact actgcttttg ctacacgcaa gagatcatct ttcaagatga
     6421 ccgcatctgc actttcacta gcagctgtag aaccatgagc ccccattgca ataccgatat
     6481 ctgcagcagc aagtgctggt gcgtcattca cgccgtctcc taccatcgca acaggtcttt
     6541 gatctttagg gatactttct aaaatggtca gtttatcttg aggcaagcta cgggcatgta
     6601 cagaatctaa ttggactttt tctgcaatcg tttgagcaat ggcagatcca tccccggtga
     6661 tcatcatcaa atttttcaca tcaagttttc ttagctcctt gattgtcttt gctgcttctg
     6721 ggcgaatctg atccgaaaat aggatcgttc ctgcaaaaga tcgatcaatt gttacataga
     6781 ttgctgtatt ggacgtttct atttcttcag ctgaaacaaa agaggcacgt ccaatcttta
     6841 cttcatgatt attgactact gcttggattc cttgaccggt cacttctttc agttgactaa
     6901 caggatacag ttggctgttt ttcacgttgt tcactaacga acgagctaag atatggctag
     6961 attcctgttc tgcactagct gcatacagaa gtagctcttc tttttttatt ccattgactg
     7021 gctgaatcgc ctctacttcc aatatccctt tggtaagcgt tcctgtttta tcaaaagcaa
     7081 tcgtttttgt acgtgcgagc ttttcaatcg ttgttccagt ttttacaacg atcccgtttt
     7141 tacttgaacg gctcattcct gcaactaaag caatgggcgc agctagaatc agtggacaag
     7201 gtgatgccac taccagtacc tcagcaaatc gtaaagggtc ttttgagaac caccaagaaa
     7261 taccaccgat caaataagca atcaacgtga aagggactgc ataacgatca gctaaacgga
     7321 caaatcgggc aggcttttct tttgattctt cgactagttt aataatcatt tgatactggc
     7381 tgtctgcggc acgtttttca gcgatcattt ggatcgatcc gtctccattt aaagaccctg
     7441 acataatctg atctccaggt ttcttggttt ccgggcggga ctcacctgtc aaagaagatt
     7501 catcgaccga agattctcct ttggtcactc gaccatctgc gggtacaagt tcgccaggct
     7561 tgactagtaa ctggtcatca atatttattt catcgattac tatatcctgc aattttcctt
     7621 ctttgaatcg atgggcaatt cttggggtgt tatctaacaa agactgcagt tctttacccg
     7681 ctcgtttatt cgcgtaatcc tctagactat ctccgcccgt aagcatgatc aatatcatca
     7741 aactagccca gtattctttg acggctaatg ttgcaaggat tgcggtaatc gctaatacat
     7801 ctacgccata tcttcctgaa cgtaacgtct tgatcatttc caaaaacatc aagaaggcga
     7861 gaatccctcc agtaaatgcg acaatcgcaa atgcaagggc cggctggtga aaaacaaatt
     7921 cacttaataa agctatccca ccaataagca aagtaagtaa aaattttttg aaatgtgtca
     7981 ttgttttttc tcctctttta tgtgacttag agaggttagt aataaatttc cagctgcctc
     8041 tttcatctta agcatagtcc ttgactagca ctttttaaaa caataggcct taattgaaaa
     8101 caactatcac tatcagcgtt tccaaaattc atcagttcta caaacaaaaa tattcagcaa
     8161 ggattacttt ttcgattcat cagttaaaaa gtaccttttt tcgaaaaact atgctatact
     8221 tttaattcgt tatttacggc ggaaggagca aacatgcgat gaatgagaaa caactagaac
     8281 aaacgcacgt ggatgaaacg atcgaactaa tccagatcga acaaaatatg ctggatacaa
     8341 aacaacaaga attgactcag gaaatgaatc attcgacaaa agacacagct aatcacaaga
     8401 ttcgagctgg ttccaatgaa gctttttatg aatctgctgt tgaatatcgc caacatgagc
     8461 aggaattgct tttgaaatac catactcttg aagcacagca aaaacgcctg caaacgttgt
     8521 ctgtcatgaa agatagtcct tattttgccc gtatcgactt taaagaagac cgtgaaacag
     8581 aaacattata tctaggtatc gcttctttaa gagatctggc agaagaaaca atcgttattg
     8641 actggcgcgc accaattgcc aatctttatt atgaaggtga gattggtccc gcattttatg
     8701 aaactgaaac agatagaata gatgtcgatc tgcagttgaa acgacaattt aaaatccaag
     8761 aaggacgtat tttatctatg gtagatacgt cggaagtaat caatgatgac tttttgctag
     8821 agatattgga tgaatcatca agcaaccata tgaaaaacat cgtctctacg atacaaaaag
     8881 cacaaaatgc aatcattcgg gacacaacaa gtaaagtaat gctgattgaa ggaatcgccg
     8941 gaagtggaaa gacatccgct ttgcttcaac gtattgcttt tctgttgtat cataatcgca
     9001 agtggctcga ggctgaaaac gttcttctat tttcacccaa ccatttgttt tctgattaca
     9061 tctcgactgt attgccttca ttaggtgaaa gcggcgtgcc tacacagaca ttcaaaaatt
     9121 atttggtgca gctgctgcca gagttttctt tactagatga acagcaacaa gaaagtggtt
     9181 ttttgctagg ggaaaaagat ccgatacaag tgatgaaaag cggattgacc ttagtcgatc
     9241 agattgatcg ctatatccaa ggcatcacaa actacggtcc cttgttccgt gatatgaaaa
     9301 taaatggccg tacgatccta tcaaaggaat cgattcgcca atggtataaa gaaacaaatg
     9361 aacaattacc tttacatcat agactttctc tattacaaac gaagctcctg aaaaaattag
     9421 gcggtctgca aaaagacgaa acacgtcagc aatgggtcaa agatctggcg gaagaacagc
     9481 tccaagaact atacgcaaca gatccaaatc ttgaatatac agaacaaaag gagcgttcat
     9541 tacgtaaaaa actggccaac caaatcgtac gtaaaagatt ccgtcgaatc catcgaggga
     9601 tcatgaatta tcaatttgtc aatataacga aacaatatct ccactttcta caaactgtgc
     9661 cactttctct tctaacacga cataaaatca gcgagaccag gtggcaggaa catttgacaa
     9721 ctgttcgtac acacttgcgt caaagagaat tgcgtcaaga ggatgctgta ctattctttt
     9781 tactgatgcg ccgaacatat ccagtgacgg ttacacacaa agctcgctat atctttattg
     9841 atgaaatgca ggatttcgca ccatcacaag tggcattgct gcaagccttg ttcccaaaag
     9901 caaatttgac tttctgtgga gatctaaatc aaaatgtctt tggaaatgaa acgatcgttg
     9961 gatcattgga agaccttttc gtggaccgtg aagtcactcg tttccaatta acgacaagct
    10021 atcgctccac aaaagaaatc actgattttg ctaatcattt tattgtgaaa gaacatcaag
    10081 tagaaacaac agcaagaaaa ggagaccttc ctttgctcat ccaaggaaca acagatgata
    10141 tcaaattgaa ttggttagca gataccattc gaacgactca gggaaaagcc cggtattggc
    10201 gtacagcaat catagctaaa acacaagaag aatgcgaagc tctttatgct caactgccag
    10261 acgagttgaa acaacaagtc cagctgattt taaatgagag tgactttatg aagcgttcca
    10321 ttattttact tccagccttt ttagcaaaag gtctggagtt tgaccgagtt ttcttgtggg
    10381 atgtagatgc agaacatttt tcttctcagc aagacaaatt gatcctttac accatgtgta
    10441 cacgtgcgat gcatgaattg acccttttta cctccaataa tgaaagtcct ttgttaaaca
    10501 atatggatat ttcatcttat gagaaaaaag tactgtaagt aaaaagacaa ctgctatcat
    10561 taatgttagc agttgtcttt ttatttacaa taataattgc agaaaaagta attcaatcat
    10621 ttaaggtcat ttattctata tataaatcca aatctgtttc aggatatttt aatttgacaa
    10681 gcgaaataat cattttcagt tcttccatgt tttctggata ttctgggtca aataccttgc
    10741 tatgacggcc aatcccgcca ttgtcgatca atcggtaaat tcgattccat tcaatgaatt
    10801 ggtcaattga ttggaaattg ctgattggat agccgccaca atccaaaatc tctgatacac
    10861 gaaatgctag gtatgcacta tcgatcactt tatttttttt cgtgtctatc ttctcatttc
    10921 tttcgataag aattttttca ggacgatgtt tcaagctcca ctctaatagt agaaagaaag
    10981 cctccatccg atactcttct tcaaataacc gaaaggctga atcagaacga cgaaaaagca
    11041 gcggaatcag ataattttgt ctataaatca gcattggtgc gcggtccacc atacactcta
    11101 aataacggtt cagataccct gtaccgacat agtttcctaa tcgatagcct tcaacgccga
    11161 atcttgcgta tttttgcgct aattttgaac aatcagttac gttcacagct aactcttcta
    11221 ctttgcggta accgcttaag atacgttcaa aaggttctaa agtgattttt gcttccatat
    11281 tcttccccgt ccatcttcca catacttcat atcatttcac atgatactta tgctctcatc
    11341 ataccacagg ttgaattaga aaagaaaata aatcacacaa aaaagcaaga caatctgtta
    11401 gtgagattgt cttgcttttc gaaaaagact gaatactttt cgttaaaact ggttgcttag
    11461 tcttctaatg ccacattatg gaacacatgc tgtacatctt ctagatcttc cagtgcatca
    11521 atcatttttt caaatgtttc ttgatcttct ggagatagtg ttacctcgtt ttgagggatc
    11581 atttggatct cagcgattga aaaatcagtg attccttttt cttttaatgc ttcttgtata
    11641 ctatggaaat ccgctacttc accgtaaacg atgatttgtc cttcttcttc cattacatcg
    11701 cgaacatcga tgtctttttc cattaggtat tcaaggatct cgtcagcgtc ttctcctgca
    11761 aaagcaaaga ttgctgtatt gtcaaacatg taagaaaccg ctccggaaac acccatgttc
    11821 ccgccatttt tcccaaatgc tgcccgtacg tctgctgctg ttcgattcac attgttcgtc
    11881 aatgtatcaa caatcaccat tgagccgctt ggtccaaatc cttcatagcg caattcagag
    11941 tatgtttcat ctcctgcacc ttttgctttt tcgattgctc ggtcaataat atgttttggt
    12001 acattataag ttttagcgcg atcaatgacg aaacgtagtt tttggtttgc atgcggatca
    12061 ggatcacctg attttgctgc cgcgtagatt tcaatcccaa attttgcata aatccggcta
    12121 ttattagcat ctttagcagt tttctttgct acgatatttg cccatttacg tcccatattt
    12181 tcacttcgct ttctgatttg atagtttcat aacgcaaaaa cgtcctgttc cattatactt
    12241 gtgaatcacg attctgtgaa gctttttttc tttcttcctt cattcttctc atttttagtc
    12301 aattatctat actcggattg ggatttgccc gaaattctgt tccggaatat tgcctcccaa
    12361 ttgatagatt tgatcacttc taactaacag ttccgcttct tgtgcctctt tttttccgat
    12421 tcgggaataa atagaaatct cttttttttc ttttaagaat cttcttccag catcggtaaa
    12481 tccaagtagt cgaagataat ttttattttg ttggacagga atctcttgct ttttgatatt
    12541 cagcaagaca taacaagata atcgttggat ccgtgtccat gtatatcttt ttgtttttac
    12601 ctttgacaca aaatcctcaa aactatcagc agttgcagct gctgttttca tgcgcgattc
    12661 tattccttcg gtcatctgat agatatctgt cagttccagc aaagtagaag ataacaaccg
    12721 ataacgcaaa tacggccaat aagattccca agtctgcagc gaacaagttg aaagatcttc
    12781 ttctgtctga ggcggcacaa gtccagctat tctttttcct tcttctaacg catggcgaat
    12841 ggctgttgca ctggctatcc tctgatctgt gatgattttt tcatgatagc ctgcactttt
    12901 acgtcggatc ggatgtaatt ccatagggtg gacatacttg gcattttcct ttgcataact
    12961 taatcccaag atatgatttg gcgaagaaaa atcaagcgta atatcgggat acagactgcg
    13021 aaaaacctca gtcatttttt gcggataact atacgtttga tcagacagtt cttgaaatgc
    13081 ctcttctatc ctatcttgat tctcttggac aaagcgtccg aaactgctgt aatcaaacga
    13141 agtctcttct tctgttccaa aacacaaact gtcacagtcc agtgtttgca agatttttac
    13201 tgcaccttta gcaaaatagt cagctgactg aaccgcccat tcgacgggaa gctccactac
    13261 taaatccaca ccattttgta aagcttcatt cgcgcgatgc catttatcga tgatagccgg
    13321 ttcgcctctt tgcaaaaaat tcccgctcat tacagcaatg acgacctctg ctcctgttat
    13381 ttttcgtgcc atttccacat gatagcgatg tccattgtga aaaggattgt actctacgat
    13441 aatcccgcag cttttcatgc gcatcctcct tcatagttat cgataaaaaa gaaatagaga
    13501 caagtaaaaa aaggcaaaaa tcggaaaaaa tacccgaact atataaacta ttctcgcaaa
    13561 cacaatactt ctgcgacgaa aagctgcact gcaagagcaa tgcctatatc gttcggatat
    13621 ataatttatg ttgaagattt attcttttct tgttaactcg agcaggcaaa accgctagtc
    13681 tttgtatgct acaaagaacc agcgctttga tttctctgat gctgcttcgt ctgtaaaatc
    13741 agaaaatgct tcgactttcg aaaagccgct gttttccagc attcttaaat aactttccaa
    13801 tggataggta cgctcttgat gtagttcatc ttcacgaata aatcgaccag acccttcttg
    13861 ttcttcaacg aaaaaggtaa gaaaatgttc gatgctgtgt ttctcgcttc ctggataact
    13921 atcccacaaa aaagcaaaat tatccccttg ataatgatag ctatattctg gaaaaacctc
    13981 atctacttga taggtggagt gtacgtcaaa taaaaatcgg ccattttctt ctaacgcttg
    14041 atagacttca tcaaacactt gctgtacctc ttgtctattt ttcatatagc acagcgaatc
    14101 agaaaaacag gtaattgcct gatattgtcc gatttctgat aaatcgagca tattgccttc
    14161 cacaaattgg atcgatgcgt cttcttcata tgctcgctgg ctggcaatca tcaacatctc
    14221 ttctgaaagg tctaaccctg tcactttata tccatctttt gcaaaggcaa ccgctaatgc
    14281 tccagtaccg catgccagtt ccaaaatatt ttttgtgtct tcttgtaaat gacgtttcga
    14341 aaaagctaac cattgctgat aaagtgtttc gtccatcact tcatcgtaaa tgaaagcaaa
    14401 tgtttcataa gccatatgtt aatccaacca ttcactaaga tcgactagcg gagcatctga
    14461 ccatagtttt tccagattat agaaatttcg ttctggcgca tggaaaacat gtacgatcac
    14521 atctccaaga tcgatcaata cccattttcc gccttctttt ccttcaatac gttttacttc
    14581 atatttgttt tcttcttctt tatcaatgat ttcttctgta attgcgttga tttgacgttc
    14641 gctatttgct gaacagatca tgaaataatc tgccaacaag gaaacatttc gaacgtctaa
    14701 agcgataatg tcttctgccc gttttgaatc tgctgcttca atcgcaattt ttaattgttc
    14761 tttactatct atgatgattt cctcctaata tgagcagctt atttttttgc tacccagtaa
    14821 ttatatgttt cgattgtttt tggataaatc tgttgttctt gcgcaagcaa atgggccaat
    14881 gtatgttttg tttcaaatgc tacagcttcg tctagatcag cccatgcaat ggctcgtgca
    14941 tcttgtaccc caggaaaatc tcgacctggt tcaatatagt ctgctacata aatgattttg
    15001 tcaaggagac tcatcttagc agccccagtc gtatgcaccc ggattgcatg aagaatctct
    15061 gcatcggtga ttcctaattc ccgctctaca aacgaagcgc caacaagacc atgccagatt
    15121 gcattattat aattcaataa ctccggatca aaaccgtctc ttacgattac cattttgaac
    15181 tcctcatctg gtcgttcttt tgcataatca tgtgtcaatg cagcaatact agctttttca
    15241 ggagaagcac cgtatttttt ggcaagagcc acggctgttt cttctactcc aagcacatgc
    15301 ttgaagcgtt gttcgctcat tctcatctgt attttttgca tcaggttttc tctagaataa
    15361 gcagtatatt cattactata gtccatattc atacaaccct tcattttgaa tataatcaat
    15421 tactttatct ggaacaaggt agcggataga acagccttct ttgatctttt gacgtatttt
    15481 cgtggaactg atgtcgatct caggtacatc tacccaaatt acaggatatg gagtatccgt
    15541 tgtgtatcct ggtcgacgta tacccacaaa gttgaccata gaagtcagct catcgatttt
    15601 ataccatttt ggcaaatatt ctaccatatc cccaccgata ataaagtaat aatctgtatc
    15661 cggattgttt tgcgtcaatt ccttcatagt atcataggtg taacttttcc caccacgagc
    15721 taattcaatc gtttcaatct gcaaaaaagg attgtcttcc accgctaatt ccaacatgtt
    15781 gagtcgatgt tttgcatcaa tcgtctgttt ctcatcaaca tgaggtggct gataagaagg
    15841 catcaaaaat acgcggtcaa gacctaaatt ccggccagct tgttcagcca tcactaaatg
    15901 tgcaaggtga acgggattga atgttccgcc caagatccct acttgctttt tcttctctaa
    15961 gaattgaggc atttcttcag gaaataattg tgaacgaaca aatgtcttag cttgcgtatt
    16021 catcatggtc actccttata ttgcttttac ttcttttgat agcctttgat acttttcttt
    16081 tgtggaaggt ttgtataata caataacacg tccaatgatt tggacgattt cgcaatctat
    16141 ttctttttct aatacttgag ctgcttcttc tgcaatctca tctgtatttt gaagtaacga
    16201 tactttgatc agttctcttt tttctaaagc ttcttcaatt tgaacgatca ttgctgaatt
    16261 gatcccacct tttccgattt gaaaaatcgg ctgaagatga tgcgctttgc tacgtaaaaa
    16321 acgcttttgt tttcctctta aactcataat tttccttctt ccttaaatca atgctttgcg
    16381 tttcaataca tctacaccct ttggcgccca gcccgcaatt actactggcg ctgttacagt
    16441 gatccagcct agaccggcaa aaacgatgtc tgttttttct ttcacagaaa actcaaaacg
    16501 aacaagctcc ggaaatgcct cgacttcatc aggacgagga ggctgtaata atccacctac
    16561 atgtttttga taaaattcgt ctgctttttc cagtttcgtt cgatgaatat ttacatcatt
    16621 tgacacataa gcaataaatg aagcacgatt gccgctgata aaatcaaatc ttgcaagtcc
    16681 gcccaagaaa agtgtttgcc cttcattcag ctgataaact ttaggtttga tttcttttaa
    16741 cggtgcaatg atccgcaaat cttttttacc taagtagtgc gccatttgat ggcgatggat
    16801 aattcccggt gtatcaatca aaaaatgccc gtcatctaaa ggaatctcga ttttatccaa
    16861 ggtcgttcct ggaaactgcg aagttgtgat tacatcttgt actcccgctg tatttttgat
    16921 gatttgattg ataagcgtcg attttccgac atttgttact ccgacgatat agacatcccg
    16981 tccatcgcgg tattcttcga ttttttctaa tagttccgtc atctcatgag atttcttagc
    17041 actagttaat aatacatcta ctggacgaat tcctgcttcg taagcacgtt ccttcatcca
    17101 ttgtgttaat ttagtttttt tcaatgattt aggtaatata tccactttgt tcccaactaa
    17161 aagtactgga ttgtcaccga caaaacggtg tagtcctggg attagtgaac cattgaagtc
    17221 aaaaatatcc accacattga cgatcaatgc atctttttgc cctaattcat tcagtaagcg
    17281 caaaaagtcg tcatccgtca atgagacatc ttggatctca ttataatggc gtaatctgaa
    17341 acaacgctga caatagactt ccccggaagc taaacctttt tccaaagccg tttgcggcgt
    17401 gtatcctaat tcaccgggtt tgtttgtttg tataacggca ccacagccga tacattgtaa
    17461 ttcttctgtc attcgattcc acctcgccag cccatatcag ggtgttttgc taatagatag
    17521 gccataattc ttttttccca aaaacgatta aattgtgtct tccaactatc tgttgcgata
    17581 atgggtttta caaggatgct acgtatccct gcacgattgg ctccacgtat atcagtcata
    17641 atctgatcgc ctaccattac tatctcatca tctgctaaat ccaattgttt ctttgctatg
    17701 ttgatccctc ttgctaaagg tttcattgct cgtgcaacat acaaaagatc aaatttttca
    17761 atcgctcgcg cgacacgttt tgaattattg tttgaaacaa caacaacagg gattcctgca
    17821 tttttcattt ctaaaatcca agtaagcaac tcttctgttc catctggatt attccatgca
    17881 atcaacgtat tgtctaaatc ggtcaatact gctttgatgc ctagattctt taattgggca
    17941 ggtgtaatct gatagatggc ttctaccatc catgttggtt tgtaatttga aaacatgctg
    18001 tctcctcatc tgcaaaaaat gaaggagcac ataaagtact cctcaacccc gtattatact
    18061 gcatatcagc aaaaaaaact acctctatac tcgttcgtct ttcagaaaaa gcatttcttt
    18121 acaaaatgac caaaaataaa gattgatcac agaaaaattt agaaacaaaa aacaactaat
    18181 attggcggaa cctcttgtat cgtttttcca gtaatttatg ctgatctaat cgaacaagtt
    18241 ccgtcaatct ttcaatcaac gcttttttca taatgtgtgc gactttatat cgtggcaact
    18301 gctcgccgtt gaacgtttca ggaataactc gatcaacgat tcctaactcg cgtagttctg
    18361 gcgcagtaat tttcatcaac tcagctgctt ctgcggcgcg actaccgtct ttccacaaaa
    18421 tcgacgcaaa tccttctgga gaaagcacag cataaatcgt ttcttccagc atccagactt
    18481 catccgctaa acctagagca agggcaccgc cacttccacc ttcaccaatg atgatggcga
    18541 tgatcggtac ctttaaatca gacatttcga gcagatttct ggcaatagcc tctccttcgc
    18601 cgcgctcttc tgctcctaca ccgcaatatg cacctgctgt attgatcaag gtaatcaccg
    18661 gtctattgaa cttttccgct tgtttcatca gtcgtaaagc ttttcgatag ccttcaggat
    18721 ggggagaacc aaaatttctg cggatatttt cctgcagatc ccgtcctttt tgaataccga
    18781 tcactgttac cggtttgcca tctaatcgag cgattccacc aacaatcgct tcgtcatctg
    18841 caaataaacg atcaccatgg cattcctcga aagaatcgaa gatctcttca aaaaaatcca
    18901 agcttgtcca tcgttcttta gctcttgcta actgaacgac atcatgtgct gtcttatcca
    18961 cgatctatcc atcctttctg tgtatgcaat tcaagaagcg tatggatttt ctgtttcaat
    19021 tccatgcggg gaacaatctg atcgacaaaa ccatgagata ataagaattc agccttttgg
    19081 aaatcttctg gaagttcctg cttgatcgtc tgttcgatga cgcgtcttcc agcaaaaccg
    19141 atcaagcttt ggggttctgc tagaataata tcgccttcca ttgcaaaact ggcagttact
    19201 cctccggtcg tcgggtccgt taaaacagtt agataaaaaa gaccttcatt gctatgccgt
    19261 ttgacagcag cagagatttt tgccatctgc attagagaaa agattccttc ttgcatccgt
    19321 gcgccacctg aagctgtgaa caagacgact ggaagacttt cctttgttgc tcgttcgaat
    19381 aatctggtga tcttttctcc gacaatcgtt cccatacttc ccatgataaa attaggatcc
    19441 atcactccaa taatgaaggg aatcccctga atggttgctt taccagtgag gactgcttca
    19501 tgaagtcctg ttttatcttg cattttttta attttttccg tatattcggg aaatgctagc
    19561 ggatctttgg ttacaagatc cgtatcccat tcttcaaaac ttttttcatc aataatcaaa
    19621 gctagacgct gccatgcacc gattcgaaaa gcgtatccgc aatgagggca tactttttct
    19681 tcaccgatgt ctttcgtata aaggatatgt ttgcaatttg gacactttgc ccacatgtta
    19741 tcaggaacag atggcgagtt agcaggtttg ctctctctat taggattgat ccgaatatat
    19801 ttctttttct taaacagagc catctatgcg cctcctatct ccgtttctgg tgtccaattc
    19861 ggtaaaaatt cttcttgtaa aaatgctgtg ctgtaatcac ctgctatgac tgcaggatga
    19921 ctaatcaaat ccatctgaaa ttctgcgttc gtgatgattc cttctgtgat aaattcactt
    19981 aaggctcgct gcatcttcat taatgcttca aaacgcgtat ttccatggac gattattttt
    20041 gcaatcattg aatcataata tggcgggatc gtatatcccg ggtaaactgc actgtctact
    20101 cgcagtccca tcccacctgc tggaagcagt aagttttgga tcttccctgg agaaggagca
    20161 aaatggaaag caggattttc tgcattaatc cggcattcga ttgcgtgtcc ttggaatact
    20221 acttcttctt gacacacatt caaaggctca ccggctgcta tctccacctg tttttttact
    20281 aaatcgattc ctgtgaccat ttctgttact ggatgttcca cttggattcg agtattcatt
    20341 tccatgaaat aaaaatcgcc ttcttcatcc atcagaaact cgatcgttcc ggcattttca
    20401 taattgactg ctttagctgc tcgaacagca gcgtctccta gtgcttctct ttttgtttgt
    20461 gagatcacta cagaaggaga ttcttccagt actttttgat tgttgcgttg taaagaacaa
    20521 tctcgctcgc ctaaatgaat gacatgacca aatcgatcac ctaagatctg aacttctata
    20581 tggcgagcag gataaatgat tttttccaaa tacatatcat catttccaaa tgccgctgct
    20641 gcttcttgct tagctgaaga aaaatgctgt gggagctctt cttttgattg aactttccga
    20701 atgcctttcc cgccaccacc tgcagctgct ttcagcatca ctggataacc aattctgtct
    20761 gctatttcca atgcttcttc tactgtagat aatacaccag tacttcctgg aatgactggg
    20821 acatttgctt cttgcatcaa ttgacgcgca ttgatcttat ttcccattgc atcgatcgtt
    20881 gcacttttag gaccaataaa tgtgatattg cactcttcac acatggaggc aaatcggcta
    20941 ttttcagata aaaaaccaaa tcctggatga attgcttcgg cttttgtcac gattgcagcg
    21001 ctcagtactt cctgaacatt cagatatgaa tcaacggctt ttgccgggcc aatgcagatt
    21061 gcttcatctg ctaactgtgt atgaagtgct tcggcatctg cttctgaata tatcgctacg
    21121 gtttggatcc ctagttcgcg gcatgcacga atgatacgta cagcgatttc accgcggtta
    21181 gctatcaata ctttagaaaa catatttcac ctatccaatc ataaaggtta attccgcttc
    21241 ggctactttt ttaccgtcta ctttagcgat tcctttgcct attccggctg aagctttcac
    21301 tttcaatatc tcaacttcaa ggtataaggt atctcccggt gtaactttct tacggaattt
    21361 tgctttgtcc aatccgccga aataagccgt ctttcccttg aattgttcaa gagaaagtaa
    21421 tgcaactgct cctgcttgag ccattgcttc aacaatcaat acccctggca taacaggttc
    21481 ttctggaaaa tgtccttgaa aaaagggctc attgatcgtt acatttttct tagcaacgat
    21541 ccgttctcct tcaaccattt cttccactcg atcaatcaaa agcattggat aacgatgtgg
    21601 aatgatttct tttatttctt gtatgttcat tgttttccct ctttcctgct ttgcttattt
    21661 ttgctgaatc ctaaagagtg gctgtccata ttcgacaact gactcgtttt ctacaaggat
    21721 ttctgtgatc actccgtcgg cctcactagt tatctcattc atcagtttca tcgcttcaat
    21781 aatgcagata acttctcctt tggcaacttg atcacctact tttttgaaag ctggtttgtc
    21841 gggcgctggt tgcagataaa cgattcccac gattggcgaa acaacctcga ttccatcaaa
    21901 tactgccgat tctttttctt tcattatttc tggagcagtt gttgcttgtt caggtatgac
    21961 attgactggc tccgaaactg gcaacgtttc gtttgtttga accatattca tgcctcgact
    22021 tgttttattt ttattcatat acaattcaaa ttgaccttca cgcaaatcga attccgttag
    22081 cgaggattga tcaaattgac tgactaattc tctgatttca ttgatatcca aaattattcc
    22141 tcccagcgtt tcatgcacag aaccgcatta tgtccgccaa accctaatga attactcatt
    22201 gcataagtaa aacttgcagg tttgctttcg tttttgacaa ctgtcaagtc aatctcagga
    22261 tcttgttttt ctacattgat cgtaggtggg ataaattgat gttctaatgc cagcaaagtt
    22321 gccaaggctt cgatcccgcc ggctgctccc aataaatgtc cagtcatcga tttcgtgctg
    22381 ctgacgtaag tatctctgaa attttcacct aatgcgattt ggattgcttt tgcttctgcc
    22441 tggtcattgg ctggtgtact tgttccgtga gcattgatat atccgacttg ttccggctgt
    22501 acaccagctt cttccatcgc aagcttcatt gctttagatg caccagaacc atctggagta
    22561 ggagctgtca tatggtaagc gtcacagttt gcaccgtatc caacgatttc tccatagatt
    22621 ttggcaccgc gagcttgtgc atgttctaaa gattcaagaa ctagaacgcc tgctccttca
    22681 cccatcacaa aaccattccg attttcgtca aacggaatcg aaccacgatt aggatcagtt
    22741 gattctgaga gtgctgttaa agctgcaaat ccagcaatcc ccatctcaca aatcgttgct
    22801 tctgttcctc cagcaagaat gacatcagaa tagccatgtt tgatgttacg gaatgcttcc
    22861 ccaattgaat tcgttccgct agcacaagct gttacgatag ctgtacaaat accttttgca
    22921 cctacacgca aagcaatatt tccagctgcc atgttggcaa tcgccattgg tacgaacatt
    22981 ggagaaactc gttttgcacc tttttcatgt agacgaataa cttggttttc gattgttgga
    23041 agaccaccga tcccagatcc gatgatgacg ccaaaccggt cttgattcat tttttcttta
    23101 tctaaaccgc tcatctccat tgcttctaaa gcagcgtaga ttccatagat cgaaaacata
    23161 tccattcgtt tgctatcttt tttaataaaa tatttatcgt aagggaaatc tttgacttca
    23221 cccgctaagg taatccctgt ttctgaagca tcaaatttag tgatcggtgc gatgccattc
    23281 gtcccctctt ttaagctatt caaaaagctg tctgcgtcat ttccaatagg tgaagtgact
    23341 ccatagccag taattaccac tcgattcatt tgtttgctct ccttttctta tccgtgcatg
    23401 accaatccgc catcgacatt gatgacttgt ccggtgatat atggattttg tgctaagaaa
    23461 actgctgttt gtgcaatatc ttctacttgt ccgaaacgct gcatcggaat ttgcttttcc
    23521 gcttgttcct tgactttgtc tgctaaaact tccgtcatat cagttgtgat gaatcctggc
    23581 gcaatggcat tacatgtaat gcctctagtt gcagcttctc gtgccacaga ttttgttaat
    23641 cctactacgc cagctttact agctgcataa tttgcttgac caatatttcc aatcaaccca
    23701 gatacactag agagattaat gatggcccct tctcgctgct tcatcatttt tttcaatacg
    23761 tgctgcgtca tattgaaggt tccaatcaaa ttgatatcca gacatttttt gaaatcttct
    23821 gcatccattc gcatcacaag tttgtcattt gtgattccgg cattgtttac taacacatgg
    23881 atcgatccaa gcttttcttc tgcttctttg atcatttgtc ccgctttctc ataatcagag
    23941 atgtcaccgg aaactcctac gcattttaca ccgaatgctt cgatttcttc tatcttttct
    24001 tttggaatct ctccgcgacc attcaaaatg atatttgctc cagcttttgc aaaagctaag
    24061 gccatcgctt tgccgattcc tcttgtacta cctgtgataa atacattttt tccttttaca
    24121 tccataacgt tcctccttat agtattgcta ttgtttcatc aaatgtctgc ttattctcaa
    24181 cacgcagaac tggtattgtt ttatcgattt ttttcataaa tccgcttaat acttttccgg
    24241 ggccgacttc gatgacttgt tcgatgccga ttgtttttaa cttatggata ctctcataga
    24301 aacgaacagg ttgcatgact tgctgttcaa gtagtcccgc gattgtttct ttcttcatta
    24361 tttccgttgt tgtgttgctt atgataggaa aagaaggttc agagaattga atctgactta
    24421 ggtccttcgc cagttttttt gctgccggtt ctaaaatagc tgtatgaaaa ggcccgctga
    24481 catttagagg gatcattctt ttgaaccctt tttcttttaa tagtaaaaca gcttcgtcta
    24541 ctgctttttc ttcgccacca atcacgatct gctgaggtgt attataattt gcaggagata
    24601 cgatgccgta cttgctagct tcatgacagc tttcctcgat cgtttcaatc ggcgcattca
    24661 tcactgcaac cattttcccg cttccagctg gggcagcttc tgtcatataa gcgcctcttt
    24721 ttgctactaa cgcaactgct tcagtgaagg atagcgcatt actagctact aatgccgaat
    24781 attctcctaa actcagtcct aaagctgcat ctgggatgat tccatgctcc ttcaataagc
    24841 gataatacgc gatgctgact gttagaatag ctggctgtgt atattgcgtc tgatccaaac
    24901 gttcattttc tgtaaagcaa agttcagcca tatcgtagcc gagaatctca cttgcttcat
    24961 caaatgtctg ctttacaacg gcttcttcat ataaatcttt tcccatacct tgatattgtg
    25021 caccttggcc actaaataag aatgctgttt tcatgttttt ctccaatata tccagaatat
    25081 atgtttgatt cttcctattc aaaaaggccg agacattcct atttcaggaa atgtcccagc
    25141 ccccaaccta attttaatga ttatgcagtt tgtttttcta cgtaagtcac taaatcttga
    25201 accgttttga ttccgtcttc tgtttcgatt ttcacgtcaa attcatcttc aatttcattg
    25261 atgatttgga ataaatccaa gctgtcagct tctaagtctt cttggatgtc agtagtcaat
    25321 ttgatttctt cagtttcttt tcctaattct tctgcgatga tttcttgaat tttttcgaat
    25381 accataataa tatattcctc cgatattgtt tgctttttat ttttataatt taacagtcta
    25441 caactccatg agaatacttc cccatgttaa acctccgcca tagcctgtaa atattacttt
    25501 ctgctgtttt cctaaggtga tctttccact agaaactgct tcatccaata agatagggat
    25561 gcttgcagca gatgtattcc catatttatc catattggtc aggaactttt cttgcggcat
    25621 ctttactttt cgggcaattt tctcaataat tctgaggttt gcctgatgca aaagcagata
    25681 atcaactggt ttgtctttca tgacttcttg gatattgttt gcaacgtctc gtacggcaaa
    25741 atcaaaaata tctctgccag tcatttgcaa ataatacgaa ccttctgaat cttctgaata
    25801 gaacggattt ttattttgct ggtagccagc ggtcaatgag ctacttctgg taccgtcagc
    25861 tgctaatttt tctttcagca attttttctg tggattcgct tctaagagaa tcccacctgc
    25921 tccatcgcca aacaacacag cagtcgtacg atcagaccaa tcgattagct tagatagtgt
    25981 ttcaccacca atcaccagtc ccttagttga gccgcagcgg atcatttttt cagccatgct
    26041 taacgcataa acaaatcctg agcacgcagc actgatatca aaagcgatag catgagttgc
    26101 tcccaatttc ccttgtactt gtgctgctac agatggtgtc gtgtaatcgg gcgacattgt
    26161 tgctactaaa ataaaatcca aatctttagg gtttagattt ttctgtttta tcaatttttc
    26221 cgccactaaa atgcataaat cagaagtctc ctgatttgtt acacatcttc gttcacgaat
    26281 acccgtcctt gacgaaatcc attcatcaga ggtatccatc atcttggaaa gatcattatt
    26341 agtaataatc ttttcaggaa ggacgctggc tgttgcagta atcattccaa attgttccat
    26401 taaatatcct cacttatagt cttgcaagaa gtcgtgcaag tttttcagtg catccagcaa
    26461 ggcatgctgt tcctccgtag ccattccgtc taagatgttc ttgaccattt cacgatgaaa
    26521 atgctgatgg acccgaaata gtaactttcc ttttttcgtt agtcccaact ttacaacgcg
    26581 gcggtcatct tcactgcgga tacgttcgac atagcctttt tttactaact tgttaatagc
    26641 agtggtcaag gtcccaacag taattgatag ttctttggct acttctgaag ttgttttttt
    26701 cttgtacatc ccgattgcct cgatcgtgtg catctctgtg attgacagat ctttgaattg
    26761 agattttttc agctcagact cttcgattgt caaaatatca ttgaagacac taacaagata
    26821 atcatttact gtttttaaat ctggttccat gttccaccgt ccactaatta gtttgattat
    26881 caaatctttt gatgttcaaa ctatatctta acttttttca aaatgcaacc ttaaaagatc
    26941 atttttttac tatatgagct ttttttaaga ttttactcat ttaaaacaac tgataaatca
    27001 gcatttataa tttcttttat tagaaaaatc atctatctca catattttga ttatcaaagt
    27061 attttctgtg aacagaacta tagaattccc taaaacttgt ccattatttc aaaatttgtt
    27121 atagtctttt tattcaaagc gtaaaaggag atcctatgtt aaaagaaaaa atgaaaactc
    27181 tccccatgct gccgggtgtc tatcttatga aagaccgaga tggtaaggtc atctatgttg
    27241 gtaaagcaaa acggctcaaa aatagagtaa gcagctattt tcatcagaat aaacagcatt
    27301 caaaaaaagt attacgtatg attcatcata taacagattt tgatttcgtc gtagtagaca
    27361 cggaattaga tgctttattg ctcgagtgtc aattgatcca acattaccgt cctttttata
    27421 accgacaaat gaattatttc agtaattata attatgtaca tattaccaat aagggatttg
    27481 tattaacaga tactcctacg gctcgaacat acgggccttt tcgtctctat aaaaaaatgc
    27541 cgtctatttt gcgtataatg gaagaaactt atcaaatgcc ttggttgtct gaaattagtt
    27601 tgttagcttt gcgagtccag cttcctgacc tacaggaaat gacttttgaa cagaaaaaaa
    27661 aggaactcca agggctgttt caaggccgaa ataaaaagct actgacttac ttgaaaaaaa
    27721 gacaacaaca tttcatctat cagttgaatt tcgaaaaagc cggtatgctt caaaaagata
    27781 tcgaattagt tacttatttt attagacgga tccaggaaca aaagcagttt ttaagaacac
    27841 catcgctcac cttcagcatg ccattagcag cagatgagag tcagaaaaag cactacctcg
    27901 tttgttatgg tcaacttgca gaaacaatga ttacctctgg tgacgtttct cctgacttct
    27961 attatgaaaa gaaagaagct catttatctt tgaaacgtca attgtcaaaa gaagaaatcg
    28021 atcctgtaca aatcttgatc agttaccgca aaaaattaga aaaagaacag tttgaaattg
    28081 aactgttaaa caaaaaagaa gctgaaaagc aactaaatta gttgcttctc ggcttctttt
    28141 aggcatcttc aacttcaaat gtcaattatc ctaaaattta ttggattaag tcatagattt
    28201 ccatcgctac gatatcgata ttatcaaatt cgtattgctg atgagtatct gtttcagtta
    28261 attcaaatgt atcggtcgct ttatcataag ttactgtaca cttctctacg ccttcttttt
    28321 caaaacggcg aacttgcagt tcattatctg ctgactcgac catagcctcc agtcgtttta
    28381 tgattgcgac taattgtgaa tgtgtcatcg gaggtcctcc ttttcttatt tctgttatat
    28441 tgtatcaaaa gcatagccta cttgcattga ttttctctaa aaaaactcgt tttttatgat
    28501 tttttttctg gtatttttcc ttcaggccac caaagtttga tcaaagcttg ggttccgtca
    28561 ttctcaaaac ctttatctgc caactcttca tatagtttca atgcttgggt agttgctggc
    28621 aatacaagat ccattttttc tgcttcttct aatgcaatct ttaaatcttt gataaaatgt
    28681 ttgacaaaaa aaccaggtgt gtaatcttct tttaaaatac gaggagaata attgcttaaa
    28741 gaccagttag ctgcacttcc acctgaaagc gtctcaatca ctttctttaa atcaagtccg
    28801 tttttttgtg catagacaag catttccgtc attcctgtca tcgtacctgc aatcatgatc
    28861 tgattagcca tttttgtatg ctgacctttc ccagcagaac catgcaatgt aaatgtcgta
    28921 ccaaattctt tgaataaagg cagtacttga tcataaacgg cttcttctcc accgaccatg
    28981 atcgtcagtg taccattctt tgctcctaag tcaccaccgg ataccggtgc atctaacgca
    29041 tctgcgcctt tttcttttgc tgtttttgca attttttctg ccaggcttgg tgtacttgta
    29101 gtcaaatcaa ctaaaatatg tcctgagata tctgcagaaa aaatccccga ctgtccatag
    29161 tagatttctt ctacatcttt ggggtaacct accattgtaa agagaatatt actttgttct
    29221 gctaccgctt ttggtgtatc tgcccagata gctccttctg ttactaaagg atcagctttt
    29281 gcttttgtcc gattatagac agctacttcg tgtcctgctt ctagtagatg gcgtgcgaca
    29341 gcacttccca tgacgcctgt tccgataaaa ccaagtttca ttttttcgac ctcctaaaaa
    29401 tcaaaaatcc ttgcttctat gctagtatac gctagaaaca aggatttttt cataaaaaat
    29461 gtttgaattt tatcttttaa atgaatttaa ttttattttg attcgttcaa tcgagtcttc
    29521 ttcttctttt cttcgcatct cttcttcatg ataaacaacc atgtcttgat aaaattcgat
    29581 catagcttcg ccgaaatgtg tagatgaaat atcataaagt ttagcttcta aaacttttgg
    29641 atctttagga acttcactgg aaatataatc aataagtgct ggtgcaaagt cttcgtcggt
    29701 ttcaaaagtt ttccctaatg aaggatgatc gaacaatcgg ttcaaataag catttccttc
    29761 cacgacacaa ggtgttccgg ctgccatcgc ttctgtataa gtaagccctt gtgtttctga
    29821 agtagaagca ctgacaaaat aatctgccgc tttataataa agtgctacat cattgttgtc
    29881 cacttctcct gtgaactgca caaattcttc taactgcagt tctgagatca attcctctaa
    29941 ctcttctaca taaggtcctt ttcctacgat aactaatctg gcgttaggat aagcggagat
    30001 gacctgagga aacccattaa tcaaggcttg gatatttttc tcataagaaa tcctgcttaa
    30061 agaaagaatc atcaaatggt tttcctgaag acctagtgac tcccttaatt tgctgatatc
    30121 ctgctgagtg atatcagaac gtttgaattt ttcaatgtcg ataccagtag gaatcacgcg
    30181 aagcggtgct attacaccat agctgcgtaa tgtatcaata acacgttcac ttggacaaac
    30241 tacacctgtc gtatgatttg caaataatct agacaagtat tttacatggg attgcctgat
    30301 cacttttcct ttaccgatat aatgaaggta gtcttcatac atcgtatggt aagtatggat
    30361 cagaggtatt ttcaacttct tagctaccat cttcccaagt atccctgctc cgaattctgt
    30421 atgggtatga ataagatcta gttctagttc tttggcaacc aaataagcat accacatgcc
    30481 tctaacaaca actcttcgat ccttgaaaga aataaatggt acacttggca ttcgaatcac
    30541 atctttttcc aacttatccg cattaggatc ggtcgttgta aaaatataga cttcatgccc
    30601 atgtctttct aattcttgtt ttaatgtttt gatcgatgta gcaactccgc tgacttgcgg
    30661 aaagtaggta tcggtaaaaa agccgatttt catgtttttc gcctccaaac tagtccttga
    30721 cctgatttac tttttttaat ttgctagaga ctttttcatt cagtacactt tcgtatacat
    30781 ttttaagttc ttcaccgatt ttattgatac ttcgctcttt tgctgtttga taaccggctt
    30841 gtgtcgtact cggtagttct tgattggtaa tctgttggat cagtcggata aattcttcgt
    30901 ttgtcttccc catatagcag ttcttgctat cttctaacca gccattatac actggaatat
    30961 ctcgaacaag tacgttttgt ttgcttgcta aagcttctaa aacaacgatc ccttctgtct
    31021 cttcataaga agggaaaaag aatagatctg cacctgaata cgcaccttct atgatctctc
    31081 cacgaatgta accaggaaac aggacatttt caggatgatc ttcttttaca attttccgga
    31141 tgttccgagg aatcgagtac atgggaacat gaccaaacca aataaatgtg tattcgggca
    31201 tcttcttagc aatttctacg aaatcaatga ttcctttacg ttcgaaaaac aaaccgacac
    31261 aaatgattac tttttgatcc ggagacagtt tgaaatattc tctgaacttt tgttctttca
    31321 ttggatcagg gtgatatttc tctaagtcga tcccattgga aatagcttga atcggcacac
    31381 ggataccata gctttccaac aactgttttg aataaggtgt tggcgtgatc agatagtccg
    31441 ctttttggta caatcctacc agatattttt ttacgatagg agaaagctgg ttcgatccga
    31501 taaaggaatt tcggaaatct tcctctgtag aatgcgcatg atagacaact ttttttcctt
    31561 ctcttctggc tttattgacc atattatgac tattgattcc gtatgtattg atgtgcagga
    31621 tgtcataatc ttctttttca tctagtgtat aagagatacc gacttccgtc agagcacgtt
    31681 tttgatgatc aagcgcacgc ccgattccag attttgcaag catcttttca ctttcaaaat
    31741 ataataatac cttcacctta cgtccccctt cacttatata gtataacata gcctacaaac
    31801 tgtcactcaa acttaagccc tatttttgtc tattcttact gacaaaaacg ttttcttaaa
    31861 aaagaac
//
LOCUS       DACGLW010000025        31697 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.025, whole genome shotgun sequence.
ACCESSION   DACGLW010000025 DACGLW010000000
VERSION     DACGLW010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31697
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.025"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            363..731
                     /locus_tag="IXT53_001190"
     CDS             363..731
                     /locus_tag="IXT53_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HAQ4905031.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            911..1852
                     /locus_tag="IXT53_001191"
     CDS             911..1852
                     /locus_tag="IXT53_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HAQ4905032.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            1921..2652
                     /locus_tag="IXT53_001192"
     CDS             1921..2652
                     /locus_tag="IXT53_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HAQ4905033.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            2796..4976
                     /locus_tag="IXT53_001193"
     CDS             2796..4976
                     /locus_tag="IXT53_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HAQ4905034.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            4996..5478
                     /locus_tag="IXT53_001194"
     CDS             4996..5478
                     /locus_tag="IXT53_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HAQ4905035.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            5494..6255
                     /locus_tag="IXT53_001195"
     CDS             5494..6255
                     /locus_tag="IXT53_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAQ4905036.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            6386..7720
                     /locus_tag="IXT53_001196"
     CDS             6386..7720
                     /locus_tag="IXT53_001196"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HAQ4905037.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            8027..8512
                     /locus_tag="IXT53_001197"
     CDS             8027..8512
                     /locus_tag="IXT53_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HAQ4905038.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            8505..9188
                     /locus_tag="IXT53_001198"
     CDS             8505..9188
                     /locus_tag="IXT53_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HAQ4905039.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            9314..11959
                     /gene="polA"
                     /locus_tag="IXT53_001199"
     CDS             9314..11959
                     /gene="polA"
                     /locus_tag="IXT53_001199"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HAQ4905040.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            12004..12840
                     /gene="mutM"
                     /locus_tag="IXT53_001200"
     CDS             12004..12840
                     /gene="mutM"
                     /locus_tag="IXT53_001200"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HAQ4905041.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            12804..13433
                     /locus_tag="IXT53_001201"
     CDS             12804..13433
                     /locus_tag="IXT53_001201"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HAQ4905042.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            13485..13817
                     /locus_tag="IXT53_001202"
     CDS             13485..13817
                     /locus_tag="IXT53_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAQ4905043.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            14129..14620
                     /gene="nrdR"
                     /locus_tag="IXT53_001203"
     CDS             14129..14620
                     /gene="nrdR"
                     /locus_tag="IXT53_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HAQ4905044.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            14643..16016
                     /locus_tag="IXT53_001204"
     CDS             14643..16016
                     /locus_tag="IXT53_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication initiation and membrane attachment"
                     /protein_id="HAQ4905045.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            16016..16942
                     /gene="dnaI"
                     /locus_tag="IXT53_001205"
     CDS             16016..16942
                     /gene="dnaI"
                     /locus_tag="IXT53_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HAQ4905046.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            17071..17511
                     /locus_tag="IXT53_001206"
     CDS             17071..17511
                     /locus_tag="IXT53_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HAQ4905047.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            17655..18356
                     /locus_tag="IXT53_001207"
     CDS             17655..18356
                     /locus_tag="IXT53_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HAQ4905048.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            18353..19474
                     /locus_tag="IXT53_001208"
     CDS             18353..19474
                     /locus_tag="IXT53_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HAQ4905049.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            19489..20721
                     /gene="pepT"
                     /locus_tag="IXT53_001209"
     CDS             19489..20721
                     /gene="pepT"
                     /locus_tag="IXT53_001209"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HAQ4905050.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            20853..21761
                     /locus_tag="IXT53_001210"
     CDS             20853..21761
                     /locus_tag="IXT53_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HAQ4905051.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            21796..22068
                     /locus_tag="IXT53_001211"
     CDS             21796..22068
                     /locus_tag="IXT53_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HAQ4905052.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            22079..23125
                     /locus_tag="IXT53_001212"
     CDS             22079..23125
                     /locus_tag="IXT53_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HAQ4905053.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            23377..25986
                     /gene="clpB"
                     /locus_tag="IXT53_001213"
     CDS             23377..25986
                     /gene="clpB"
                     /locus_tag="IXT53_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HAQ4905054.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(26137..26808)
                     /locus_tag="IXT53_001214"
     CDS             complement(26137..26808)
                     /locus_tag="IXT53_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905055.1"
                     /translation="MIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGITFIVES
                     LLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWDKILHA
                     VSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLCDQFLG
                     MNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFESYKLE
                     KVKNT"
     gene            complement(26801..27295)
                     /locus_tag="IXT53_001215"
     CDS             complement(26801..27295)
                     /locus_tag="IXT53_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAQ4905056.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(27315..28535)
                     /locus_tag="IXT53_001216"
     CDS             complement(27315..28535)
                     /locus_tag="IXT53_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ4905057.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            28682..30517
                     /gene="lepA"
                     /locus_tag="IXT53_001217"
     CDS             28682..30517
                     /gene="lepA"
                     /locus_tag="IXT53_001217"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HAQ4905058.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
     gene            complement(30611..31693)
                     /locus_tag="IXT53_001218"
     CDS             complement(30611..31693)
                     /locus_tag="IXT53_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ4905059.1"
                     /translation="MNDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTDT
                     RVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINAE
                     FKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKIIS
                     LEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVKN
                     DGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKEDG
                     ELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISIT
                     MDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT        10549 a         5659 c         6785 g         8704 t
ORIGIN      
        1 agaaagaggt cgtgagcctg ccgtttcact ctgagtcaca aagagcaatt tcaaaaaata
       61 gcttctcatg tttgttgaat attgtgtctt gtgaccgagg agttgtcagg agaacaccgt
      121 ttaccagagg ttgtgacttg tttttagtca cgacctcttt ttttatttat tccatataaa
      181 tacacattat ttattccgaa aagtgaaagc gcaaataaca aaaacaacca acttatttat
      241 tattaaagaa aagcgtattt aagtaggacg attctgttga gagctttcgt agattttccc
      301 attttcctat ggtaaactga caaagttgag aatgttagaa cagatcgttt aggaggaaac
      361 agatgtttac gaatgaaata aaaatcatgc tttatgtgga cgatgtagaa aaaaatgcaa
      421 cgttttggag agcagttggc tttgcggaaa aagatcgtca agaaatggat gggacgctta
      481 taattgaagt aggagttgag gacagtcaaa cctcatttct gttatatgat cgagcatttg
      541 ttgagtccca ttcacctggc acagctacta gtgccccatc tttgatgttt tacagtaaag
      601 atatctttgg actatataaa aaaatgcaga cgcaatcagt cacattaggt gatatggtac
      661 agcttggaga agaatatgtc tttaactttg ctgatcccga tggcaactac tttgcggtta
      721 caggtaaata atagaaataa agactttgtt tttctgtgcg aaacaggaaa gcgaagtctt
      781 tttaattcaa ataataatat tatgtaaaca aaaaactttc catgcgtaat tatacacaag
      841 caaataaatc gtcaaatgcg aacgccttct ttatactgtt taggaacgaa aagaaggaag
      901 gaagaaatac atggattatc aagtactgct ttattacaat tatacaaaaa tcgaagaacc
      961 agaggaattt gccaaaaatc atttagcctt ttgcaaatct ttggatctta aaggtcgtat
     1021 tctagtagct gaagaaggaa taaatggtac tgtgtctgga accgttgaaa acacgactgc
     1081 ctatatgaaa gcaatgaaag ctgatcctcg atttgctgat acgatgttca agatcgatcc
     1141 aagcgaagga catgccttta agaagatgtt tgtaagatac cgtccagagc ttgtaagttt
     1201 aaagctagaa gaggacattg atccaaacca agtgacaggt gcctatcttt caccgcaaga
     1261 atttaaagaa gctattttag atgaacagac agtggtgatt gatgcacgga atgattatga
     1321 atatgacttg ggacattttc gaggggctgt acgcccagat atacgcagtt ttcgagagct
     1381 tccagactgg atccgcgagc acaaagaaga atttatggaa aaacgggtag tgacctactg
     1441 tactggtggc attcgttgcg agaagttttc tggttggctt gtccgtgaag gattcaaaga
     1501 tgtcggacag ctgcatggcg gtattgctac ttatggtaag gatcctgaag tccaaggaga
     1561 attatgggac ggcaagatgt atgtgtttga tgaacggatc gctgtcgata tcaaccaagt
     1621 agataaacaa atcgtcggtc gcgactggtt tgacggaaca ccatgtgaac gatatatcaa
     1681 ttgtagcaat cctgagtgca atcgtcagat tcttgcttcc attgaaaatg aagaaaaaca
     1741 tttaggagca tgtagcatag agtgtgcgaa acatccgcgc aatcgctatg ttcaagacca
     1801 tggaatctct ccagaagaag cagaactgcg gtttacagct ttaattaaat agaaaacaat
     1861 agttttgatt gtcgaccatt tccaatatgc gctatactag cgttgaggat ggtgatccac
     1921 atgaaatcat tgatttatgc tattggggat gtccatggca aacatgagct ttttgaaaaa
     1981 cttattcact attatgatcc aaagatccat cagcttgttt tattagggga tctcaatgat
     2041 cgaggtcctc gtacaaaaga atgtttttta ttaggaaagc aattagtcga agaaacagga
     2101 gccatttacc tccggggaaa ccatgaagaa tattttttgc aatttctgca atcaccggag
     2161 gactggtatg cgccttatat gagaaatggt ggaaaagaaa cgtttgaaag cctgattcac
     2221 ccaggggcat cggaggaata ctccccaact gaaatctcga tgatgatcca atcgagatat
     2281 tcagaactga ttaaattttt agtccagcgc cccttgtatt atgaatggga aaattatctt
     2341 tttgtccatg ccggtgtgga tttgacaaag aaagactggc gtaagacaag tctaagagac
     2401 tttatttgga taagggaacc atttcatgaa gggaaaaaca atacaggtaa gacaattgta
     2461 tttggacata cgattacacc aatgctccat ggagatatgc agacaacaga cctctggatt
     2521 caggatcata aaatcggaat ggacggtggg gctgtattcg gagggtcatt gcatggggtt
     2581 gtttttgaca aggaaggtat tcttcaagat attgaatatc agaacaacga aggaccttgg
     2641 catggaatct gactaatgga aagaatgaag gagctgtgac tttgttgcgg ctctttttat
     2701 ttttatagag aatattgttt ctttcagtag tttaacccag gataaaaagt atgatagaat
     2761 aaagaagcaa tttttagcag aaaagaggca ttttaatgac ggaaaatttg aaccagacga
     2821 tcattcagct tgtacaaaaa gaactttcag actatcgtcc taagcagttg actactgtac
     2881 ttaatttgtt aaatgaagga aacactgttc cttttatcgc gcgttaccga aaagaaatga
     2941 ctggaagtct tgatgaagtc cagattcgtg aaatcgaaga gcggtatgcc tatcttgaaa
     3001 acttagaaaa acgtaaaact gaagtgattc ggctaatcga tgaacaaggg aaactgactc
     3061 ccgagttaga agcagaaatc acccaagccg tgaaaatgca acaagtggaa gatttgtatc
     3121 gtccatacaa acaaaaacga cggacaaaag caacgattgc taaagaaaaa ggacttgaac
     3181 cactcgcgaa gtggctgatg cagcttacag atggagaggt gcaatctgaa gcagaaaaat
     3241 atattgacaa agaaaaagaa gtctcttctg ccgaagaagc gttgcacggt gctcatgaga
     3301 ttattgctga acaagtcagc gataatgcaa aatttcggac ttggattcgg tcttatacgt
     3361 ataacaaagg tatgtatgtc agcaatgtca aggatgagca agcagatgaa aaaggcgtat
     3421 atgaaatgta ttatgacttt gcagaacccg tccataaaat ggtttctcat cgaatattag
     3481 ctactaatcg tggagaaaaa gaagatattc tgaaagtatt ctttcaagta gatgaagcag
     3541 caattttagc ttacttggat cgccaaatag tcaaaaatcc tgcctctcct tcttcttcat
     3601 tcgtcagaga agcctatcag gatagttata aacgctttat tcagccagca atcgagcgag
     3661 aactgcgcaa tgaattgact gaaaaggcag atgagcaagc aatcgctatt ttcggcgaaa
     3721 acctccgtaa tcttttgttg caaccgccac taaaaggtaa agttgtcctg ggatttgatc
     3781 cggcttatcg tactggatgt aaactagcag tcgtagatgc gacagggaaa gttttagcaa
     3841 tcgaagttat ttatcctcat aaacctgcag cgcaagcaaa aagagaagct gctggaccag
     3901 cttttatcca attaattaat aaatatcaag tagatatggt agctatcgga aatggtacag
     3961 ccagtcggga atctgaactt tttgtggctg agcagttgaa atccgccgac cataaaacct
     4021 attacgctat cgtcaacgaa gcaggcgctt ctgtttactc cgctagcgaa attgcacgaa
     4081 aagaatttcc gcatctacaa gttgaagaaa gaagtgctgt ttctattgcc cgtcgcctgc
     4141 aagatccttt agctgaatta gtcaaaatcg atcctaaagc tgtcggagta ggccagtacc
     4201 aacatgacgt ttctcaaaaa cgtttggctg aacaattgga ttttgttgtg gaaacagcgg
     4261 ttaaccaagt aggtgtggat gtgaatacag caagcccgca gctcttgcag catatatcag
     4321 gattgaataa aacaactgcc caaaacattg tcatttatcg tgaagaaaac ggagaattca
     4381 ccgcacgaac tcaattgaaa aaagtgccac gcttaggacc aaaagcatat gaacaagcaa
     4441 tcggattctt gcgagtccct ggtgggaaaa acattttgga caacacaggt atccatcccg
     4501 aaagttattc tattgcaaaa gaaatattaa cttcagtcca actttcagaa aaagagcttg
     4561 gtacagaaga agcagtagaa aaattgacac gattatccgt agagaaactg gctgaaagcc
     4621 tatcagtcgg agaagaaacg ttagccgata ttttagctgg gctgacacaa cctggacgag
     4681 atatgcgtga tgagatgcca gcgcccttat tacgtacaga tgtattgagt atggaagact
     4741 tgaaaccagg gatggaattg actggaacgg tccgtaatgt cattgacttc ggcgcatttg
     4801 ttgatatcgg tgtgaagcaa gacggacttg tccatatatc gaaactcagc aaaaaattcg
     4861 tcaagcatcc tacagatgtc gtgtcagtag gagatatcgt aacagtttgg atagaacaag
     4921 tcgatacgaa aaaaggtaga atcagtttga cgatgctttc cccttatgaa gagtaacaat
     4981 gaggagtaac aaaaattgac acaagaagaa ctgcagcagt tagtagaaga gatatcatta
     5041 aaaagcttcc agcttccttt caagcaccaa gcaatgttca atccacgctt acgtacaact
     5101 ggcgggcgct atcatttaaa cgatcatcat ttagatttta atgcgaagct atttgaagag
     5161 tcagacgaag agacgattgc aggaatcatc aaacatgaac tttgccatta tcatcttcat
     5221 ctagctggaa gagggtatca gcataaagat atggatttca agtatctttt gaaaaaaaca
     5281 ggagggttgc gctatgcacc agccttgacg aagaaacaga taaagatgga gtattatcga
     5341 tgccaagatt gttctgccac gatctgccgc aagagaagaa tcgatgtacg caaatatcgt
     5401 tgtggaagat gtcatggaga aatcaaatgg attgagacaa aaagagaaga ctttaatgaa
     5461 gcacaaaaaa tcagatgaaa ggatagaagg cctttggaaa aaagtgagca gaggaatcta
     5521 aaagactgga atcagctatt aaatttttta gccgcacctg aagagggaaa ttttgaggaa
     5581 ctgacggcac ctacacttat tttagctgga aactgtcttc ctatcttagc tgataaagca
     5641 gcacaaatgt atttaaatgg ccaagtagat caattgtttt tggtcggagg ggtggggcat
     5701 gcgactagaa tcctttatga gaattttgaa aagcaaggat ttcattttga agatggcatg
     5761 agtgaaagtg aaatctgtcg tcagtatcta aaagaagtct atgatttgcc cgataaggct
     5821 tttctgatag aatcgaagtc tacaaattct ggagagaacg ccactttttc actggagatt
     5881 cttcattcct taggagctgt accagaaaaa atcttattga tgaatgatcc gacactgcaa
     5941 cgcaggactc gagcaacttt tgagaaagta tggcaaaatg aacaaactat tttcgcgaat
     6001 gctgtgccat ttgtgccgga aatccttcac ttttctgaag agatcatctt tacagcaaaa
     6061 gaactgaatc atcaatggcc taaggaatat ttccatgcgc tagtattagg agaaatggaa
     6121 cgtctccatg acgatgagaa cggctatggt ccgaaaggaa aagattttat tcctcacatt
     6181 gatatatcag aggaagtttg gtcttcttac accagaatta aagagtcaat aaaaacggat
     6241 ttttctcgta cttgaccatt ttagcaagcg aatcctcctt tccaaataaa tttggttgaa
     6301 attttcaatc gtttataaga taatgaggag cgtgttgaat gtaatagcta aaagaatgga
     6361 cgctagataa gagaagagga ttcaaatgga aaatcaaaat aaattgtatc attttgttgg
     6421 tatcaaaggt tcaggaatga gctccttggc tcttgtttta catgaacaag gtctaaatgt
     6481 ccaaggatca gacattgaaa aatatttctt tacacaaaga gatttagaaa aagcaaatat
     6541 cacgattctg ccatttaacg cagataatgt aaaaccaggg atgacgatca ttgcaggaaa
     6601 tgcatttcct gattcacatg aagaaatcca gcgagcaaaa gaattaggtt tggaagtgat
     6661 tcgctatcac gactttattg gtcattttat ccaaaattat acgagtatcg ctgtaacagg
     6721 ctctcacgga aaaacaagta cgactggact gctctctcat gtattgagtg gtgtccgtcc
     6781 aacaagttat ctaattggag atggaacagg acatggtgat ccgcaagcgg aattcttttc
     6841 atttgaggcc tgtgaatatc gccgtcattt ccttgcctat tcaccagact atgcaatcat
     6901 gaccaatatc gattttgatc atccggatta ttatacaagt atcgacgatg tatttacagc
     6961 tttccaaaca atggctggac aagtgaaaaa agctattttt gcgtatggtg acgatgctta
     7021 tttacgcaaa ttaaaagcaa atgtgccgat ttattattat ggtgtcacag aaaacgatga
     7081 tatccaagca cgaaacatcg aacgcacaac ttctggttct gcttttgatg tctatcatgg
     7141 ggatgaattt gttgggcact tcactgtacc agcattcggt aaacacaata tcttaaatgc
     7201 tttaggtgtt attgcagtag cttatttcga aaaactggat ctgaaagaag ttgcagaaga
     7261 aatgctgact ttcccaggag taaaacgtcg attcagtgaa aagatcgtag cagacatgac
     7321 cgtagtcgat gattatgcgc atcaccctgc agagatcaaa gcaacgattg atggcgcacg
     7381 ccaaaaatat ccagataaag aaataattgc agttttccaa ccccatacgt ttacgcgtac
     7441 gattgcattg atggatgaat ttgcagaagc acttgattta gctgataaag tttacttatg
     7501 cgatattttt ggttcggctc gggaagaaca aggaaacgtt aagattgaag accttggagc
     7561 gaaaatcaaa aaaggtggag aagtcatcaa agaaaataat gtatctccat tgttagatta
     7621 tcatgatgca gtagtaatct tcatgggtgc tggagatgtt caaaaatttg aacaagcgta
     7681 tgaaaaactt ttaagcagca cgacgaaaaa tgtactgtaa tacaattact gcagtgtcct
     7741 tataaaaata gaaagagggg gagtggcaaa aatcttgcca agcccccttt tttgcaactt
     7801 atgataaacg gtgttcaaaa gcttgcatct gcggtaataa gcccaatcaa atcaaaaaca
     7861 tgagaagctg ttttttcttg gctgttctta ttatttgatg caggacagct ttttcacgac
     7921 ctctgtaaac tataggaatc tgttaaatca agggagcaga acttgccttt tacgctactt
     7981 ttggtacaat aatgtatgat taaaaataaa ctggagggtt tctatcttga acataggtaa
     8041 gccgagaaaa ctaggaaaaa caataaatga aatcgaggaa ggcgactcgt tatccttaac
     8101 agaatctatt gaagataacc agcttttgct gtatttggga ttgacgaatg atgcaaatcc
     8161 tttgtatatc caacatgact ttgcacaaaa aacagaatat caacagccaa tcgttccatc
     8221 agtgatgctg atggggatca tcacaagtgc aatttcaaag catcttcctg gaccaggatc
     8281 acatgttgtc aatttttctg tcaattttat tgaaccggtt ttccattatg aaacactgac
     8341 ttttcagttt gaggtcataa aagtagataa gatgaaggat gtggtgacga tttctgtaga
     8401 agcggagaat acgaacgaag atcgtgtgtt ggatgctgtc gttatggtcc aaccgccaca
     8461 agttcctgaa ttggaaatag caatcgaaag tgaaggtgaa ttgaatgaat aatccaagta
     8521 catcaatgtc aggtatcaat cgtttttatg caaaagttta tggattttta ggtatgggac
     8581 ttgcccttag tgcagtcact tcttacttgg ctctttgggt atttcctttg caattagcaa
     8641 cttttatcaa taattttcca ctaggctttg tgggtctgtg gatcgttgaa ctgattttag
     8701 taatggttct aggcgtaaaa gcacaaaaga atccgagctt agcaatcggt ggatttattg
     8761 cctactctct tttgaatggc ataacattag cggttactct tgcatactac gatattggaa
     8821 cagtaacgca agctttcttg acagctactt tgatgttcat ggggatgtct gttgtcggag
     8881 taacaacaaa aagagatctt agcggggttg gaagagcagg acaggcagct ttaatcggag
     8941 tgatcattgc actagtcttg aacatcttca ttttgagaag tagcgtagtt gagctgttca
     9001 tctccctact gttagtcgtc atctttgcag ggattacagc ttatgataac caaagcatcc
     9061 gtaatatcta tgtccagaca aacggaacag cacatagtgg aattgctgta ttcatggcat
     9121 tgaagctgta tttggacttt atcaacttgt tcttggcgtt tctgagaatc tttggacgga
     9181 acaactaaaa aagaaaaaaa ttcatcagtt ggggctatac cgaattattt ggtatggtcc
     9241 cgctttttct tttcgcattc cgcaggattc tttgttagaa tgaataagaa ctttacagta
     9301 aggagttaag aagatgacaa aaaataaatt attactcgtt gatgggaaca gtgtggcttt
     9361 tcgcgcattc tttgcgcttc acaattctct tgaacgtttc aaaaatagaa acggtcttca
     9421 tacaaatgca atctatgcat tcaatacgat gtttgaaaat gttatgcaaa aagagcaacc
     9481 gactcatgtt ctggttgcgt tcgatgcagg aaaaacgact tttcggacag aaatgtatgc
     9541 ggagtacaaa ggtggaagat ccaaaacacc tggcgaattc aaagaacaaa tgccatatat
     9601 acgtgatttg ttgacaggac taggggtaca gtattacgaa ttgcccaatt atgaagcaga
     9661 tgatattatc ggtactttgg cagaaaaagt agacaaagat caatttgatg tcgtcgtgct
     9721 ttcaggtgat cgggatttga ctcaattagc tagtaaagaa gtaaaagtgg atatcactgt
     9781 aaaaggtgtc agcgatatcg aaagctatac accagaacat gtagcggaaa aatatgatgg
     9841 gttgacgcca aaacagatta ttgacatgaa gggactagcc ggagatgcct ctgacaatat
     9901 ccccggtgtg actaaaatcg gtgaaaaaac agctatcaag ctattgaaac aatatggctc
     9961 agtcgaagga atttatgaac atatcgatga aatgaagcaa agcaaaatga aagaaaatct
    10021 gataaatgac aaagagcagg ctttcttatc gaaaaaattg gcgaccatca atacttcagc
    10081 gcctgtggat gtctctatcg actcattgaa gtatgaaggg aaaaatctcg ataaactcgt
    10141 tccgttttat aaagaaatgg atttcaatca gttcctttct aaattgaata tcgtagaaga
    10201 agaagtaaaa atggatgata ttttgtttga agtcgttcat gaattcaaag aggaaatgtt
    10261 tactacggat atggcgttgt acgtggaaat gatgggcgac aattatcata cagaggaaat
    10321 cgtcggagta gcgtggggga cagaaaagaa aatttatgtc acgaacgatc tatctgtatt
    10381 cgaaaaccga gcgttccatt cttggatcac tgatccgact cggctgaaaa aagtatatga
    10441 tgcgaaacga acttatgttg ctttaaaccg ctatactgga aaaacgaaag gaatcgattt
    10501 tgatgtcttg ctaggtgcat accttctcga tacgaatgaa aaaagcacag atatcgaagg
    10561 aatagctgct cattacggat acaacgatat ccaatcagat gaagctgtat acggcaaagg
    10621 ggcgaaaaaa ggtttaccag aagaagagga actctttttt gctcatttag cgcgaaaagt
    10681 agctgcaatc aatgcgttga caccaaaatt gagccaagaa ctggcagaaa aaaaccaaga
    10741 agatcttttc cggaaaatgg aactgccatt gtctatcatt ttggcagaga tggaaataag
    10801 cgggatcaaa gtagatgcga ctcgcttaca agaaatgaag ggcgagttct ctgctcgctt
    10861 aagagagatc gaacaaaaaa tctacgaaga agccggcgaa gaattcaatt tgaattcacc
    10921 aaagcaatta ggagtgatct tatttgaaaa aatgggatta cccgttatca aaaaaacaaa
    10981 aactggatat tccacagccg tagatgtact ggaacagctt cgtgaacaag caccaatcgt
    11041 agaggatatc ctcacctatc gtcaaattgc aaaaattcaa tctacctatg tcgagggact
    11101 gctgaaagtg atccaatcag atggaaaagt gcatacccgt tatgtccaga cattgaccca
    11161 aacaggacga ttaagctctg ttgatcctaa tttgcagaac atcccgattc gattagatga
    11221 agggagaaag atacgtcagg catttgttcc tagagaaaaa gactggctga tttattcttc
    11281 tgactattca caaatcgagt tacgcgtact tgcgcatatc tcaaatgatg aacatttgaa
    11341 agctgccttc ctagaaggtc aagatatcca tgccagcaca gcgatgcgtg tatttggaat
    11401 cgaaaaggca gaggatgtca caccaaatat gcgtaggcag gcaaaagcag tgaattttgg
    11461 tatcgtgtat ggcatctctg attacggact ttcacaaaat ctaggaatca gccgaaaagc
    11521 agcacagcaa tacatcgata cgtactttga aaaataccca ggtgtcaaag aatatatgga
    11581 aagaatcgta cgcgaagcaa aagaccaagg ctacgtcgaa acgctgtatc accgccgtcg
    11641 atatctgcca gacatcaatt caagaaatta caatatccgt tcttttgcgg aacgaacagc
    11701 tatcaataca ccaatccaag gaagtgctgc tgatattttg aaaatcgcga tgatcgaatt
    11761 ggataaacga ttaaaagaaa caggtcttca agctacgatg ctgttacaag ttcatgatga
    11821 acttgtcttc gaggtaccgg aaaaagaact tgaatcatta gataaattgg tgaaggaagt
    11881 tatggaacaa gctgtttctc ttcatgtacc actgatcact gacagcagtt ggggaaaaac
    11941 gtggtacgaa gcaaaataaa cattgtttaa gacagagcaa tataatgaag gtgaaaagaa
    12001 aaaatgccag aattaccaga agtagaaacc gttcgtaaag gattggaacg gttagtagta
    12061 ggaaaaacga ttcaaaaagt tcaggtcttg tggcccaaga tcatcgagca accagagaca
    12121 cccatctttg aggcttctct tgttggcgaa accatccaat ccattggacg tagagggaag
    12181 tttttaattt ttcatctcga tcattgtgaa ctgatctcac acttgcgaat ggaaggaaaa
    12241 tatcaattta cgaaagaaaa tacaccaatc gataaacata cgcatgtact tttctttttt
    12301 gaggatggaa gccaacttcg ctacaacgat gtacgaaaat ttggtcgtat gacaatcgtc
    12361 gaaaaaggag caagtgcaac ttaccgagga atcatgaaat tgggacctga gcctttgcca
    12421 gatagcttct tattagcaga tttcgcaaat ggactgaaaa agtcccacaa agcaatcaag
    12481 ccattgctgc tagaccaacg tctagtgact ggtctaggta atatttatgt agatgaagca
    12541 ttatgggaag cgaagatcca tccagaacaa ccggcgaaca cactgcgccc caaagaggta
    12601 gaacaattac gtctatcgat cattgatgtt ttggatcgtg caatagaagc cggaggtacc
    12661 acgattcgca gttatttgaa tgcattagga gaatcaggcg gatttcaagt agcactgcat
    12721 gtctatcagc aaactggcaa accatgtata cgttgtggaa cgccgatcgt gaaaacaaaa
    12781 gtggcacaaa gggggacgca ctattgtccg aattgtcaaa ggctaaaaat cgcaaagtag
    12841 gatttgtttt aggtttgacg ggtggaatag ctaccggtaa aagtacggca gccaaagtct
    12901 ttcagtctca tggatttcct ttgatcgatg gcgatgtgat tgccagagaa actgttgagc
    12961 ctggaacgct tgctctgagg aaaattgtat cagtgtttgg ccaagaggta ttgcagcctg
    13021 atgggcaact agatcgaggg aaacttggga tgattgtttt tccatcaaag gaaaaacggc
    13081 aaaaacttga tcagctatta gatccgttta tccgaaaagc gataaaagat caaatcgcct
    13141 ctctaagttc caatcatcct ttagtaattg tagatatccc attgctctac gaaggtcatt
    13201 atgaccacta tatggatgca gtagctgtcg tttatactac gccagaaact cagcttcagc
    13261 gtttgatgaa acgtaatcaa ctgacgctag aacaagcgca gcagagaatt agtagtcaac
    13321 ttccaatcga agaaaaaaag cagcgtgcgg atattttgtt cgacaacaat ggaacaaaag
    13381 aaaaacttgt gggacaaatc gaaaattggc tttttgctaa tcgctttctt tgagtatctg
    13441 tgtcaatttt gctatcctga gtatatagaa agtgagggat agcgatggga tcattaaaaa
    13501 atcaaaagga taaagtcgta ggtaacgcaa aagaaaaaat cggcgagatg ctcaataaag
    13561 aaaatctaat cgatgaaggt cgtacgcaat cagaaacagc agaagaaaaa gaaaagcagt
    13621 accaagcaag aatcgatgaa atagaagacc agcaggcaga agaagaagcg aagcggcaag
    13681 atttgactca agaacgaaaa aatccgacac ctgatcgtag taaaacaccg ctcccagaaa
    13741 gtgaccacat tcccgatgag caaaaaatcg aggaacagcg aatgaaacac tatacaggaa
    13801 tcgaagagaa gttataaaat agtaaaaaat cgagatgtga cttgaagcag atgagaaggt
    13861 gttcatctgc ttcaagtttt ttgtgctaga tgactaaatc agcttttgtg taagttattt
    13921 ctcagtaatc aaggtgatca gtattggaaa attgaaattt agtaaggaca ggggacacaa
    13981 ttacgcatga aagtagaaaa aattgttctt atatccgtgt tttttgggta ggacagtcgc
    14041 gtttgtgatt tgtttcgtgt tataatacaa ctagttgtaa agaaagacac atcctttata
    14101 taaaatgcga tgcaagaggt gagacaaaat gagatgtccc agatgtcaac ataataattc
    14161 acgagtaatc gacagccgtc aagcagatga tggacgtgcg atccgtcgcc gtcgcgaatg
    14221 tgagaattgt tcgttccgat tcactacatt tgaacgaatt gaagctgctc cgttattagt
    14281 aatcaagaaa aacggggaac gcgaagagtt caatcgcgat aaaattttaa gaggactcat
    14341 ccgttcagca gaaaaaagac ctgtagctat ggaacaaatg gaacaaatcg tcaaccatgt
    14401 cgaaaaccgc gtacgcagtc tcggagaaaa cgaagtatcg acgaatctaa ttggtgaata
    14461 tgtgatggaa gatttagttg atttagacga aatcgcttat attcgttttg caagtgttta
    14521 tcgccagttc aaagatatgt ctgttttctt aaaagagttg caagatatcg tggataaagc
    14581 aaaagaaaat aataaagaga ataataaaga aaacaattaa gaaacaaaaa ggaggcattt
    14641 ccttggaaaa tgcttggaaa gagcttcaac caaaaaatat ttaccaagga agaaaagcgg
    14701 ctccgctgag agaagacagc aagaacgcct tgctgtatct ttatcagcca ctaatcggcg
    14761 gagaagcctt gagtatctat ttcaccctgt tatcagaaat ctctcaagaa tctggcaaag
    14821 gtcctgaagg gatgcatgca gatctgctta gtagtctagg ctgcggtatc ccgcaatttt
    14881 atgatgcaag aaaaaagcta gaaggaatcg gcttactgga tgtttatttt aaagaagata
    14941 gtgatctggg acctcgtttt atttatgagc tgttagaacc actcattcct aaagcatttt
    15001 tcaaagactc tgttctggct tttcttcttc tggaaaaagt tggtgaacga cgattccaga
    15061 agcttaccca attctttgaa cctaaaaggc atacagcaga gggatatcaa aaaatcacaa
    15121 aaaaattttt agatgtttat cgcttcaatg aagcagtgta cgctgctaat caagaaaaca
    15181 tcgaatcgct gaataaatct tttgaagaat ctgaatcagt agcattactt gaagcagatt
    15241 cgacattcga ttggcagttt ttgaatgatt ggctggcaaa aaaacatatt gcaagtcttg
    15301 atgatccaat catcaaacaa gtcgacatgt atcatcagct ttacggtttc gatgaattga
    15361 cacttggcga actgatcagt caatcttatg actttagtga acaaaaagta tctgaaaaag
    15421 aactgcaaaa agccattttg gcaatgcaga gacgtccggt gaagcaaaca aaagaaacag
    15481 ttgcggtttc tgaaaacagt aatgaaccct cgtcaaacga acatttgaca aacgcagcgc
    15541 ttcagctgat aaaagaagca cagagcattc caccgatgaa atatctggaa gcaatcaagt
    15601 cagaaaaagg cggatatgtt tctaaacaag aaaactggtt actgcaagat cttgtttctc
    15661 aatcaggtct ttccagtagc gtgatcaatg ttttgctgaa ttatgtacta gtgatcaaga
    15721 ataatgctag cttaaatgcc agttatgtaa atacagtggc aaatgaatgg gcacaaaaga
    15781 aaatcgccac agcagaagaa gcgatccagc acattcggca aataagtaat caagcaaaac
    15841 aatcaaaaca aaagaagcaa acaaattatc aaactggcaa gcgaaatgtt cgacgggaaa
    15901 agcttcctga ttgggtcaac cagccaaaag atgaaaaaca gatcagcagc gagaaaaaag
    15961 cagagattga ccgccgtttt aaagaatacc tggcaaaaaa ggagggtgat atctagtgga
    16021 agacgtaggc aaagaattga agaggatcat cagcaaacgc aattatcaag aaaagtatgc
    16081 tgaaatgatc gctgaagtct taaatgatgc ggatgttcaa gcttttctaa gcgcacacca
    16141 agaagaattg acacaagcgg atattgaaaa aagttacgct aaattgtatg aattcgtgca
    16201 agaaaagcgt aaatatcaaa aaaacgatcc aacgatgatc gctcccggct atgagccgaa
    16261 gctgacgttg aattttcatt atgtggatgt cacttatgta ccaacggaag cattgttagc
    16321 acggcaaaga caagaagaaa tcagaagccg agtaaaagca atggatatgc caaaagatat
    16381 tcaatcggct tcttttgaaa actttgaacg tacagatgga cgaggatatg cgagcttaga
    16441 agccctagat tttgtagaga aatatactgc tgatccaaaa gggtatcata aaggactgta
    16501 tctagtagga agttttggta ttggaaaaac atatttatta ggagcaacag cgagagaatt
    16561 agcgattgct ggattcacca cgacgctagt ccattttccg acatttgccg ttgagatgaa
    16621 acaggcaatc ggaaaagatc aagtggcaga aaagttagat gcagtgaaac gttcacctat
    16681 tttgatgatc gatgacattg gtgcagatgc gatgagtagt tggatacgag acgaagtatt
    16741 cggtgttatc cttcaatatc ggatgcagga acagttacca accttcttct catcgaattt
    16801 tacaatggat gaattggaac agcatttgtc cgtcactcaa cgtggagatg aagagccgct
    16861 aaaagcgaaa cggataatgg aacgcatacg ttatttgaca aaagaaatcg aaatgactgg
    16921 tcgcaaccga cgcaatggtt gaacagttga caagtccaac tgacatttga cctagtagta
    16981 aaagcaggtc agcgaatcat tatatggaca gaaatgaccg gagacaatca ccgcaacaag
    17041 taatgcagaa acttaaacgg aggatttttt atgagggcta ttttttctag tatatctgta
    17101 gttggtctat tttttctttt agcaggctgc ggtagcgcag atagcaacac aggttctgtt
    17161 gattcgattg atgcgggtca aacagtaaaa acaagcagta cgaaaacgga gagacaaagc
    17221 atatttgtcg gaacgattga aagtatcact caagtagaag cagaagaaga aacgcttcag
    17281 ttgaaactcg tcgatgtcat ggccaaagaa gatccagcga atatgggaac gtcattcagc
    17341 aatgacggag tgatcttaaa tgctactgct gaaaaaataa gcgtggacat aaatgaattg
    17401 aaaatcggtg acaaagtcca atttacatta gttgagatgc cgatcatgac gatgtctatc
    17461 ccaccgcaag ttcctggaga ttcgattatt gatgtttcat tgaacaaata accaataaca
    17521 ttttttcaaa aataagaaaa tgttgtttta tagaacgaaa aggtatattt tttaaggaga
    17581 tatgggaatt tttttattcc tagtctcctt attttatttg gaaaatgcta taattatcta
    17641 gaaggagtga agctatgaac catttagatt tatctcagcg cttagccgca gtgggtgact
    17701 ttgtccctga atccgctcgt ttagcagaca ttggatcaga tcacgcttac ttacccgttg
    17761 cattgatatt aagaggaaaa atcgaatatg cagttgctgg tgaagtcgtt caaggtcctt
    17821 atgaatcagc acgtaagcaa gtcaaaaaag atggattaga agaccgtatc accgtacgcc
    17881 ttgcaaatgg tctagatgct atccagccac aagatggtat tacagctgtc acgattgcgg
    17941 gtatgggagg ttcactgatc cgagatatcc tagaagctgg caagttgaat caacgcttaa
    18001 gcggacaaga acgtttgatc cttcagccaa acatcggaga gcgtacatta cgatcttggc
    18061 tgcaagaaaa tcagtataaa atccaatcag aaaaaatact tgaagaaaat aaaaaaatat
    18121 atgaaatcat tgtagccgaa aaagaacaaa aaccagttac ttacaatgaa atggagctga
    18181 tgttcgggcc atttctgctt gaagagaaaa gtgagatctt caaatcaaaa tggacaagag
    18241 agcttcaaca aagaaaagct atccgtgaac agctcaaaca agcagctaat caagaggcac
    18301 gtcaaaaaca aatcgagcag gaaatccaaa tgattgagga ggttttaaag ccatgacaat
    18361 cagcggacgc actttcatcc aaaagtttga aagctattgt cctcagtggc tagctgaaga
    18421 aggagaccca gtcggactgc atatcggaac attggataag ccaatccaac gagtaatgat
    18481 gacattagac gttcgtccag aagtggtcga agaagcaatc aagaaaaaaa tcgatttgct
    18541 gattgctaaa catcctccaa tctttcgtcc tgtaaaacgg ctggttactg atcagccaca
    18601 agaaaaaatg tatgcagatt tgttgaaaca tgatattgcc gtctatgcag cacacacgaa
    18661 catggatatt atttgggatg gactgaatga ctggttttgt gaacttctcg gtattgaagt
    18721 ggagagttat ttagtaaaga ctcatgagat tcattataaa aaactggccg tttatgtacc
    18781 tgtggatcat gcacagaaaa tgcgtgaagt attagctgca gctggtgctg gtacacaagg
    18841 tgattatacc ggtacaagtt ttacctcgat tggacacggt cgatttactc cggaagctgg
    18901 tgcacaacca gcgattggga aagtagggaa gacagaacaa gttcaagagg caaaagttga
    18961 ggttattttg ccggaaacta tcgaaaaaca agtgattcaa gccatgcggt cagctcatcc
    19021 ttatgaggaa cctgcttatg atcttttcgc tatagatgaa ccagtcgaaa tgtttgggtt
    19081 aggacgagta ggtgaattgc cgcaagaaat ttctatagag gcatttgtag aacaagtaaa
    19141 agaagcgttc caactagatg gactgagaat agtccagcca aaaaatgcaa aatcctctgt
    19201 gaagcgcatt gcgatttgcg gtggaagcgg tgaaaaattt tatccgcaag caatcgccca
    19261 aagagcagat gtctatatca cgggagatat ctattatcat acggctcatg atatgcaaag
    19321 cgctgggttg attgcaattg atcccggtca ttatattgaa tcgttatgca agcaacgatt
    19381 tatcgagaaa tttgaatctt ggaaacaaga agaaaattgg gatattaact ttttcgtttc
    19441 tgaaacaaat acaaacccat ttcaatttaa ttaaaaggag agaatactat gtacgaaaat
    19501 cttttgccac gttttttacg atatgtcaaa acagaaacac gctccgatgc aacaagcaaa
    19561 acgactccat cgactcagac acaagttgct tttgcgcaag aattgaaaaa agaattagaa
    19621 gagttaggct tagctgatgt tttctataat gaagataacg gattcgttat gggaacatta
    19681 ccaagcaatg ttgatcatga agtgcgttcc attggattta tttcccacat ggatacagca
    19741 gattttaatg cagtaggagt caatccacaa atcattgaaa actatgatgg cgagtctacg
    19801 atcgtactag atgcggaaga acgctataca ttgaatacaa aagattttcc aaatttgaaa
    19861 aattatgaag gccacacatt gatcacaaca gatggcagta cattactggg agcagatgac
    19921 aaatcaggga ttgcggaaat catgactgca atggagatct tgatcaataa tccttctatc
    19981 cctcacggta ccatcaaagt ggcttttggt cctgatgaag aaatcggtgt cggagcggat
    20041 aaattcgatg ttgagcaatt cgatgtagat tttgcttata cagtcgatgg tggtccttta
    20101 ggtgagttgc agtatgaaac gtttagtgct gctcaagcaa atattacgat ccaagggaaa
    20161 aatgtacatc cagggacggc aaaagataca atgatcaatg ctctacagtt agcaatcgat
    20221 tttcataatc agcttccagc cgatgaagtt ccagaaaaaa cggaaggata cgaaggtttc
    20281 ttccatttga tggcattgga tgggaatcct gaagaagctt ctatgagtta tatcattcgc
    20341 gatcatgatc gtgaaaaatt cgaagcacgc aaagcacaaa tcacacatat ccaagaagag
    20401 ttgaatcagc gatttgatca agaacgaatc aaagtagata tgtatgatca atattacaac
    20461 atgaaagaaa tcattgaaaa agatatgagc atcgtggaat tagcgaaaca agcaatgatc
    20521 gagcttggta tctctccagt gatcgaacca gttcgtggag gaacagatgg ttcaaaaatc
    20581 tcttacatgg gtattccgac gccaaatcta tttgccggag gagaaaatat gcacggacga
    20641 ttcgaatttg tttctttaca agtaatggaa aaagcaacag acgtgattgt aaaaattgca
    20701 gaattgaaca caaagagtta aaatcgaaaa agaacagctt atgacaaggc gttcgtccca
    20761 atgatggaat gtcatctgtc taaggctaaa gggcaggaca atggaattgg ttgtcccgcc
    20821 ctcttttttg aagagtaaat aggaggtaaa gaatggaaaa gctgctagtt ttttataaca
    20881 aaagttcagg gaaagatgag ggtgagaaat tagcacgctg gtttcaagat tatgtaaaag
    20941 aaaagcagcc agaattatat gtagaactag ctgaaactgg cccatcgatt gatgatgcgg
    21001 aattagtcaa gaaagcagaa gccatagaag ctgatacttt agtaattata ggtggagacg
    21061 gcacgattca tcatatcgtc caagcgttcc aaaaaacatt atctcaatac catgtcggat
    21121 tacttccagg tggaactgtc aacaaccttg cacgtgttct tgagattccc ctcaaaaaag
    21181 aagaagctgc tgacgtgatc ctggaaggca atgtacggaa aatagactat ggtcaagtca
    21241 atgatgaagt cattatctct acactgacaa tcggaatctt agcagataca gcagtttgga
    21301 tcagtcaaga agaaaaacag cgttacggct cgtggatctt tatcaagaag ttttttaagt
    21361 tattgctgaa gaagaaaaaa tatcatttgg atttagaaac agacgaggag cattggaaag
    21421 gaaaatccca actgttgaca gtcacgatgt ctaactccgt gggcggtttt acaaattttg
    21481 acgactcagc gaccctagat gatggaaaat ttcatgtaac gatcgtacct tccttagata
    21541 ttttccgatt tattttttat ttgcctagaa tcatgagagg caaattctat acgatccccg
    21601 gcattactta tttcacagcc aaacgcatga aaatccaagc gcaggaaaaa tctgtgggca
    21661 ctcgaacgga tggagacacg acagatgatc ttccagttga attatctgtt gtatcaaaag
    21721 gattaacagt ttattcacca aagaaagaag aaaaagctta gaaatagtag ttaacagaaa
    21781 gttggaagaa gagtaatgga tgtattgtta cacaatcctt tgtggtttta ccttttcatt
    21841 gtgaattttt acttattttg tctgatgggg tatgataaac atcaggctag aactagcggc
    21901 tggcgtgttc ctgagaataa tttgatattt gtcagcctgt taggcggtgg tttgggagga
    21961 ttgatctctc gaaaagtttt ccatcataaa actcggaaaa agaagtttac tataggattc
    22021 gctgcaggtg tacttgttga tttgattctc atttattttt tccattaagg agcaggagat
    22081 gtcgtctaaa ggaacgaaaa ctaaaatcat tctgaatctg cttttgctga ttgtgatttt
    22141 tgccattatt tattatttaa tcaatcaatc atttgcagat atctttgccg aattgctgtc
    22201 taccagtgtt gttgttttgg cagcggctgt tatattaggg acagtttacc aaatcgctga
    22261 agggcgctca atcaaagaaa ttgctgaacc tttcgcgccg aattttacaa cgaaagatgg
    22321 gttcttgacc tctagctata ttgcgtttta ccgaatcgtt tcgtttggta caggaacgct
    22381 gatctcggag atttattttt ataacaaaaa aggaatgaag ctatcccagg gagcaggggt
    22441 gacagcactc catatgatta tgtacaaact ggcagtgatc acttatgcat tagctggctt
    22501 ggtcatccaa ttctctcttt tttattctaa tggtccatca atgatctggt ttatcatcgc
    22561 tggtatcctt ttgacgatcg tgattgttgc ctttttattg gcgatgtccg tcagcttgaa
    22621 tgttcaaata ttttttgtca gcttatcgaa taagttattt aaaagtgagc gtttgcggaa
    22681 atgggtggat acatgcaatc agcagatcta ctctttaagg gaaacggtcc aaaccattat
    22741 ccaagacagg actgcgctat tgcggattta tctatggaat atgttaaaac ttgtattttg
    22801 gtatgtactt ccttatttga ttctgattga aaaccatcct aaaatagatt ttctactgac
    22861 catttcgttt atcagctttg cagtagtgct ttcaggggtt attcctacgc ctgctggtat
    22921 tggttccttt gaatttgtct atttgttatt attcagacca gttgttggaa cagttgacgc
    22981 agtttcttcg cttttactgt atcgtttcag ttcctttatc ttgccatttc tctatggatt
    23041 tgtgtatgtg attgctgaac ggcgaagagt gatcaaagaa gaaataatag aagtaaaaaa
    23101 agagaataaa gaaaatcaag ggtaggtctt ttataagatc tacccttttt atcaaatcaa
    23161 agaaaaacgt tatggataag ataattttac gtttgtttgg ctcttttttt atatacatac
    23221 aatcaatttt atttttttta agatttatat aagtcgagaa aaaaagtcaa aaaactatgt
    23281 gtatattttg accaaaattg accaaagcga tataatagtt tttgtaaaga tcaaagaaat
    23341 aagtgatctt acctttaatt aggagggtat acctgtatga atattgagaa aatgactacg
    23401 acgcttcaag aagcgattgc tgaagctcaa cagattgcgg tcactcgaaa acatcaagat
    23461 atcgacattg cccatgtatg gaaaatcttt ttacaatcta atcattttgg tcgaaatttc
    23521 tatacggatg ccggattgga tgtcgaatct tttgaacatg aaattgatcg tctactggat
    23581 gagtatccag ttgtttctgg tggaaacgtc caatatggac aaaacctcag ccagaatttc
    23641 tttcgattat tgaatgaagc agaccaaata cgtgaaagct tcggcgacga gtttctttca
    23701 acggaagtcg ttattttagc gttgatgaaa ttaaaaaatt atccgttaac tgtttatttg
    23761 aataaaaacg gattatctga aaaagaattg cgtaaaaaca tcgaagagat gagaggagga
    23821 gaaagagtga cctcacaaaa ccaagaagaa caatacaaag cattagaaaa atacggcgtt
    23881 gatttagtac agcaagtcaa aagcggaaaa atggatccaa tcatcggtcg tgatgaagaa
    23941 atccgtgatg tcatccgtat tctttcacga aaaacaaaaa ataatcctgt tctgatagga
    24001 gaacccggtg tagggaaaac ggcaatcgtt gaagggcttg ctcaacgtat cgtacgcaaa
    24061 gatgtgcctg aaaatttaaa agataaaacg attttttcat tagatatggg tgcattgatc
    24121 gctggagcaa aattccgtgg tgaatttgaa gaaagactaa aagcagtctt gaaagaagtc
    24181 aaaaaaagtg atggacgtat cctcttgttc atcgatgaaa tccacaatat cgtaggggca
    24241 ggaaaaacag aaggaagtat ggatgccgga aatctgttga aaccaatgct tgctcgcggt
    24301 gaattacatt tgatcggtgc cacaacttta gatgagtacc gtcaatatat ggaaaaagac
    24361 aaagcgttgg aacgtcgctt tcaaaaggta ttagtcaaag aaccaacagt ggaagatacg
    24421 atcagtattc tgcgtggatt aaaagaacgt tttgagatcc accatggtgt taacatccat
    24481 gacaacgcat tagtagcagc agctacttta tctgaccgtt atattactga tcggttctta
    24541 ccggacaaag caatcgattt gatcgatgaa gccagtgcaa cgattcgggt agaaatgaac
    24601 tctatgccaa cggaattaga tcaagttact agacgtttga tgcagttgga aatcgaagaa
    24661 gctgcactga aaaaagaatc agatgatgca agtaaaaaac gcttgaaaaa tttgcaagaa
    24721 gaattagctg aacttcgtga agaagcaaat gcgatgaaga tgcagtggga aacagaaaaa
    24781 gaagaagtca attctgtttc agcaaaacgt gctgaaatcg ataaagcaaa acacgaatta
    24841 gaagatgcag aaaataacta tgatttagaa cgcgctgctg tattgcgtca tggtacgata
    24901 cctcaattag aaaaagaatt gaaggaatta gaagctaaag caaaagacag cgagattaag
    24961 atggttcaag agtccgttac cgaaaatgag attgctcaag tcgttggcag attaacgggt
    25021 atccccgtaa cgaaacttgt agaaggtgaa cgagaaaaac taattaaatt gaatgaaaca
    25081 cttcataaac gagtgattgg tcaagatgaa gcagttgatg cggtcagtga cgcggtaatt
    25141 cgttcacggg ctggattgca agacccaaat cgtcctctag gttctttcct tttcctagga
    25201 ccaactggtg tcggaaaaac cgaattagcg aaagcgttag ctgagaactt gtttgactca
    25261 gaagatcata tggtacggat cgatatgagt gaatatatgg aaaaacatgc tgtgtctcgt
    25321 ctagtcggag ctcctccagg ctatgtaggt tacgaagaag gtggtcaatt aacggaagct
    25381 gtcagacgaa atccttatac aatcgtcttg ttggatgaaa tcgaaaaagc gcatcccgat
    25441 gtcttcaata tcttgttaca agtcctagat gacggacgct tgactgattc aaaagggcga
    25501 gtagtagatt tcaaaaatac ggttctgatc atgaccagca atatcggttc acagttactg
    25561 ctggaaggcg tgactgctga tggaacgatt ccagaagcag tggctgaaca agtcaacacg
    25621 ctattacgtg gaaacttcaa accagaattc ttgaaccgaa tcgacgacac gattttattt
    25681 acaccattga gcctagacaa cgtgaaagga atcgttgaca aaatggtggc gcaattggca
    25741 caacgtctgg aacatcaaga aatcctgctg acaatcagtg atgaagccaa aacgtggatt
    25801 gctgaaaatg cttatgaacc agcatatggt gcaagaccat tgaaacgatt tatcactaag
    25861 gaagtagaaa caccactagc taaagaaatc gttgcaggac atgttatgcc taaatcaaaa
    25921 gtaacgatta ctttacttga tggacaactt catttcaaaa cagaagagct agaagaaatc
    25981 gtctaatctt ccattgagac tataaaagaa aaaaggggtc tgacacaact agtgtcagac
    26041 ctcctttttt caaataaaca gtgttccaga agtagtttct tcgaaaaata agccgaaatt
    26101 tcttgaagct gcccgcttct gtcacaacct cttttattag gtatttttca ctttttctaa
    26161 tttataactt tcaaagtaac gctcatcttt tttactttga gaccatgcaa acaatcccat
    26221 taaggcagca cctatcgtat ttgtcagcaa gtcgcccatc gtatccatca atgctgctcg
    26281 tccgacaaac agtgtgccat cagaagcagc aaaacgttga agattcatgc ctaagaactg
    26341 gtcgcataag aattcccaga actcccaaaa cacgccgcaa agtccagcaa acgcaaagcc
    26401 gaataagagg aacaaccaag gactggtttt tgatatctca gcatctttca tcaagtaacc
    26461 aatcaatcca tagccaagag ctgtcaggac cattggactc accgcatgta agattttgtc
    26521 ccaaaaagag atgatactga tcaaatgcat ccctgtgcct aaaaagacag agatgaacaa
    26581 gaaaaaccag taaaacaaaa cgattgctgc tggtatttta agtttgaaaa ctttcaaaat
    26641 gacttgtggc atgaaaatca atgcaatccc taataaagat tccacgataa atgtgattcc
    26701 ttgagaagtc gaatattttt gtgtgataaa ttcataaaaa ttatagcaga caacgaatac
    26761 accaaaagca agtaaagcta tttgcacctt tttgatttct ttaatcactg aaagtccctc
    26821 catctaaaaa tctttttact tctgaaagca actcaatttc tgaagaaacc acttttccgt
    26881 tcaatttgat caaacctatt gtatataagt tcaggtagtg aaattgattt tcagctgttt
    26941 cttgtagtgc agcgattttt ttcgggtggg ttccgccttg ctgtcgagta tcagtatata
    27001 agccgatcac tgggatgcct ttcgcataag ccacaccgat ttccgatgct accccggcat
    27061 cgatcgtcag cccatctagt aaagcaatca ttaaatcact agccaacact ttttctgtgt
    27121 cagcaagagc aatcattcga ctgtctgcat aagcagtttt gtcattgatt gcttcatttt
    27181 cttgaggaag atagatcgaa agctcagaag agagctctct gatttcttta actaactgtt
    27241 ggttatataa caaatcactt ttagcaaaca ttggtgcggc aaaataaatt ttcatctaaa
    27301 catcatcctt tttatcaaaa ataatcgcta aagtaagctt ccatgtttgg aatgatacgc
    27361 tctagtgact tcagtgtttc ttcatctgtt tctaaccgtt cgattcttgc caaataagaa
    27421 gcacggcgat aattcatcat caagcaagtc aatgtttgga tatccagtcg aacggatttt
    27481 cctataggtt catttaagac acggacttgt ccctgttcat cccatgttaa agcgaagaca
    27541 ccattattcc aaggagcgac aggatcttca atgatgaaat gaaaagcatc agctgttcca
    27601 acaaacggaa accgttgaag gaattctttt acatcaacga tccgagccat aaaatatggt
    27661 tcgatctgct ctttgatctg actatcttca atcaaaaatg ctaaaggctc atttttataa
    27721 atatctccat gtacccagta gatcatggaa aaatgtgcgg aaataaagtt ccataagcca
    27781 tttcgagctt cttgatcaag atagaacatt tcttttacgt gaaatatttc ttcagctacc
    27841 cagtagaaca gaacacctct tggttcatgg ttgctatcgt agtagacagc ggctgtgcgc
    27901 tcatcttcat tttcgaaacg ccaatactct tcccaatgaa aatcactgcg gaataaagca
    27961 ccatggtttt gttgggcaaa tttgtcataa acggaaaata cgtctggatg atcaactggt
    28021 aatcgctcaa ccataccact tactgggatt tgtttaggta gctgtgtatc tctgatttta
    28081 aaggaaagtt tatcagacat gatttcccat cctttacgcc gatagtaggg gatactgtaa
    28141 ggatacaaat aagagatcca ttgcttattc ttgcgcatat tatccaatgc aacgataatt
    28201 agatcttgca tcaatccatg gttagcatat tctggataag ttccaactcc agtcacacca
    28261 cccattttat aacgtgcacc gtgaatattg acttcacaag gatagatagc gatttgagag
    28321 atcagtttat tttcgtggaa ccatccgaac actttcgata cttccaaaat cggttttttg
    28381 gaacggataa aagctcgttt attctcaaac ccgctttcct cgatgtctgc ttcagtcact
    28441 tggaacacat aggaaagcaa ttcattgaat tgatcgatat gttcttgagt gaccggtttt
    28501 aattcaagat tttctctaaa atctttttga tccatttttt catctccatt tattagcttg
    28561 ataacagtat agcaaaattc tacaaaaaaa acttctatag ctgactaatt aaaaactctt
    28621 taaattactg cttttcgttg gtataatgga aagtgctgat tatatgaaaa gaggagacgc
    28681 tatggatata aagaaaatga aagaacgcca agagcagatt cgtaattttt cgatcatcgc
    28741 tcatatcgac catgggaaat ctacattggc cgatcgtatt ttagaaatga cgcacactgt
    28801 gacgtctcgc gaaatgcaag atcagctgtt agactcgatg gatttggaac gtgaacgtgg
    28861 aatcacgatt aaattaaatg ctgtagaact caattacaca gcgaaaaatg gagaaacata
    28921 tatctttcac ttgattgaca ccccagggca cgtcgacttc acctatgaag tctctcgaag
    28981 ccttgctgcc tgtgagggtg ccgttttagt ggtcgatgca gcacaaggaa tcgaagcaca
    29041 aacattggcc aatgtttatt tagcgttaga caatgatttg gagatcctgc cagtcatcaa
    29101 taaaatcgac ctacccgcag cagatcccga acgtgtccgt caagaaatcg aagacgtaat
    29161 cggaatcgat gctagtgaag ctgtattagc cagtgccaaa tcagggattg ggattgaaga
    29221 tattctcgag cagatcgtgg aatatgttcc tgcaccaagt ggagacttag atgcaccgct
    29281 aaaagctttg atctttgact ctatttatga tagttaccgc ggtgttgttc taaatgtccg
    29341 gatcacggac ggtatggtca aacccggaga caaaatcaaa ctgatgagta atggcaaaac
    29401 atttgatgtt acagaggtcg gggtcttctc gccaaaagca gttgctagag actttttgat
    29461 ggtcggtgat gtcggttata tcacagcaag tatcaaaact gtccaagata cacgagtagg
    29521 ggatacagta acgttagcag acaatcctgc cgctgaagct ttaccaggtt accgaaaaat
    29581 gaatccgatg gtttattgtg gtctttatcc aattgatact tctcgttaca acgatttgcg
    29641 ggaagcttta gaaaaactgc aattgaatga tgctgcttta caattcgaac cagaaacctc
    29701 tcaagcatta ggttttgggt tccgctgtgg attcctcgga cttcttcata tggatgtcgt
    29761 ccaagaacgc ttggaacggg aattcaatct agaattgatc acgactgcac catccgttat
    29821 ttaccatgtt aataaaacgg atggtacgac tgctacagta gataacccgg cagattttcc
    29881 tgaacctgta acgattcagg atgtagaaga accattcgtc aaagcacaga tcatggtccc
    29941 aaatgatttt gtcggtgcag taatggaact ttctcaacga aaacggggag aattcattac
    30001 gatggattat ttagatgatt accgagtaaa tgtcgtctat aatattccgc tttctgaaat
    30061 tgtctttgac tttttcgaca aattgaaatc aagcacgaaa ggctatgcct cattggatta
    30121 tgaaatgtct ggttatcaaa aaagcaagtt agtgaagatg gacatcttgc tgaatggaga
    30181 aaaagtcgat gcattaagct ttatcgttca ccgagacttt gcctatgaac gtggaaaagc
    30241 aatcgttgaa aaactgaaaa aactaatccc acgccaacaa tttgaggtgc caatccaagc
    30301 agctattgga caaaaaatcg ttgcccgttc agacatcaaa gccttgcgta aaaacgtctt
    30361 ggctaaatgt tatggtggag atgtttctcg taaacgtaaa ctcttagaga aacaaaaaga
    30421 agggaagaag cggatgaaac aaatcggctc cgtcgaagtt ccgcaagaag ccttcatggc
    30481 cgttctgaaa atggacgagg acgaaccgaa gaaatagcaa gggtttgttt gaaaacagtg
    30541 taaaaaataa accatgttta catggatttg ccaccggttt gccaccggtg gtttttttcg
    30601 gtggcaaaaa tcaagaattc tttctaagat tttccatgat atcgaccgtt tcatttctca
    30661 tcttatctgt tacatgcgag taagtatcca tcgtaattga aattctgctg tggcccaatc
    30721 gttcagagat ttctttcatt tttgcgccat tttcgagaag aagtgtagca tgagtatgtc
    30781 tgagagaatg gaagttaaaa gagagggaga gtgcattcga tatcctccta gtattccatt
    30841 tcactacact tggcgtaact agctcaccgt cttccttcgt acatactgca tttgaatcaa
    30901 tgtagagctt tccatacttc attcgatttt ctaattgttg tttcttatgt tttttcagaa
    30961 ttgctagcaa ggtttgtcca ataaaaatcg ttcgattgga actgcttgtc tttggcgtac
    31021 catataccca tgcgccgtca ttctttacca tttgtttctc tacagtaatt gttccatttg
    31081 aaaaatcgac atcatcccac gtcagaccac aaacttcgcc aacgcgcatt cccgtataaa
    31141 atccaatatt caaaggaata tagaaaggat gtccttcagg agtaatttct agcatatgat
    31201 caaagtcctc aagagaaatg atttttagat cttttttagt cgttggtcgt gcttcatatt
    31261 ttggtatttt tacatacagc ataggatttt gcttgattaa tccccaagga taaaccgcca
    31321 tattcagcgc attcttaagg acagagtgag taatagtcat tgttttcttc gagtaaccct
    31381 ttttaaattc agcattgatg aaattttgta aaagagcagg ggagagatcc gtaagttttt
    31441 tctttcctaa ataaccgttt atatgatttt tgatggtaaa tcggtagttt tcataggtat
    31501 tgtattttag atttagttta acgtattcct ccatccaaaa atcaaggtat tgttttactc
    31561 gagtatccgt acctaaaaag tattgtcctg tttcgtcaat atctgataaa acttttcgta
    31621 aagcagcttc ggcctctggt cgggtgtctc cgccaacttt ctccactttt tttcttgagc
    31681 catcatcatt gatatct
//
LOCUS       DACGLW010000026        31596 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.026, whole genome shotgun sequence.
ACCESSION   DACGLW010000026 DACGLW010000000
VERSION     DACGLW010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31596
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.026"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            complement(112..2448)
                     /locus_tag="IXT53_001219"
     CDS             complement(112..2448)
                     /locus_tag="IXT53_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ4905060.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            2590..3258
                     /locus_tag="IXT53_001220"
     CDS             2590..3258
                     /locus_tag="IXT53_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ4905061.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            3261..4304
                     /locus_tag="IXT53_001221"
     CDS             3261..4304
                     /locus_tag="IXT53_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ4905062.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(4541..5422)
                     /locus_tag="IXT53_001222"
     CDS             complement(4541..5422)
                     /locus_tag="IXT53_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ4905063.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            5581..6309
                     /locus_tag="IXT53_001223"
     CDS             5581..6309
                     /locus_tag="IXT53_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HAQ4905064.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            6465..6782
                     /locus_tag="IXT53_001224"
     CDS             6465..6782
                     /locus_tag="IXT53_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ4905065.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            6775..7383
                     /locus_tag="IXT53_001225"
     CDS             6775..7383
                     /locus_tag="IXT53_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HAQ4905066.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            7399..8229
                     /locus_tag="IXT53_001226"
     CDS             7399..8229
                     /locus_tag="IXT53_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HAQ4905067.1"
                     /translation="MRLKHFLITGIGLMLTGGLLAGATYTLGAQKSLTWDNGPRLIDM
                     VTETKKIKDANKIVIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKK
                     STGAIISLEDTSAQITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSF
                     NEFTGGTITAKDELGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSL
                     HEMENSGYTIQTSGESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD
                     "
     gene            8367..8855
                     /locus_tag="IXT53_001227"
     CDS             8367..8855
                     /locus_tag="IXT53_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HAQ4905068.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            8906..9778
                     /locus_tag="IXT53_001228"
     CDS             8906..9778
                     /locus_tag="IXT53_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HAQ4905069.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            9954..10316
                     /locus_tag="IXT53_001229"
     CDS             9954..10316
                     /locus_tag="IXT53_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ4905070.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            10325..11077
                     /locus_tag="IXT53_001230"
     CDS             10325..11077
                     /locus_tag="IXT53_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4905071.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            11091..12893
                     /locus_tag="IXT53_001231"
     CDS             11091..12893
                     /locus_tag="IXT53_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ4905072.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            13170..14210
                     /locus_tag="IXT53_001232"
     CDS             13170..14210
                     /locus_tag="IXT53_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ4905073.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(14262..16406)
                     /locus_tag="IXT53_001233"
     CDS             complement(14262..16406)
                     /locus_tag="IXT53_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ4905074.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(16763..18136)
                     /locus_tag="IXT53_001234"
     CDS             complement(16763..18136)
                     /locus_tag="IXT53_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ4905075.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(18136..18531)
                     /gene="rbsD"
                     /locus_tag="IXT53_001235"
     CDS             complement(18136..18531)
                     /gene="rbsD"
                     /locus_tag="IXT53_001235"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002829567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HAQ4905076.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(18559..19476)
                     /gene="rbsK"
                     /locus_tag="IXT53_001236"
     CDS             complement(18559..19476)
                     /gene="rbsK"
                     /locus_tag="IXT53_001236"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HAQ4905077.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(19584..20597)
                     /locus_tag="IXT53_001237"
     CDS             complement(19584..20597)
                     /locus_tag="IXT53_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ4905078.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(20678..21277)
                     /locus_tag="IXT53_001238"
     CDS             complement(20678..21277)
                     /locus_tag="IXT53_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ4905079.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            21463..22164
                     /locus_tag="IXT53_001239"
     CDS             21463..22164
                     /locus_tag="IXT53_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4905080.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            22174..24885
                     /locus_tag="IXT53_001240"
     CDS             22174..24885
                     /locus_tag="IXT53_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAQ4905081.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            25336..26964
                     /gene="treC"
                     /locus_tag="IXT53_001241"
     CDS             25336..26964
                     /gene="treC"
                     /locus_tag="IXT53_001241"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HAQ4905082.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(27024..28205)
                     /locus_tag="IXT53_001242"
     CDS             complement(27024..28205)
                     /locus_tag="IXT53_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ4905083.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            28457..29980
                     /locus_tag="IXT53_001243"
     CDS             28457..29980
                     /locus_tag="IXT53_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HAQ4905084.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(30049..30777)
                     /locus_tag="IXT53_001244"
     CDS             complement(30049..30777)
                     /locus_tag="IXT53_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ4905085.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(30790..>31596)
                     /locus_tag="IXT53_001245"
     CDS             complement(30790..>31596)
                     /locus_tag="IXT53_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ4905086.1"
                     /translation="IIIFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKK
                     LADQLLEQQVRMQQDYIKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYD
                     QTILPTRQMMNLFPANLSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDT
                     KHTVNLVRMLGIILDNAIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLK
                     QKGFSTKTNPKNEGLGLFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
BASE COUNT        10066 a         5618 c         6389 g         9523 t
ORIGIN      
        1 taattaaaaa aaaacagcgc gaaacaagga acatattact tcctcgtttc ttgctgtttt
       61 ctactatttc agcgctctta tgactcaaat ctaaatgtgg ttgtcacaac cttattctgc
      121 acgcaaagcg atagcggcgt cttttttcgc tgccattcgt gcaggaagat gaccgccaag
      181 catcgtcaat atagtagaga cgatcactag aataatcgca tggacaggat ttagttgagc
      241 aacatttttt agatccgtca tgttgtataa gatgctattg atagggaacg tagctagcca
      301 tgcaataaac acacctaata tcccagaaga aatacctaaa atacacgttt ctgcgtcaaa
      361 tacgcgagtg atgtcttttt tacgtgcacc taaagctttt agtacaccga tttcttttgt
      421 ccgttcaatc acagatgtat aagtaatgat actgatcatg atcatacttg ttactaaaga
      481 gatccctgca aaagcaataa ggacataagt gatagcatcc atcaatccgc cagtcagttc
      541 agtcatcgtt ccagctaaat cagtataaat gattttatct tcatctgatt tgcctttatt
      601 gtaatcatct agataatcca agatcttttc tttgtcttca aaattgttcg gataaatcat
      661 gatacttgaa ggcaagctgt ctccacctaa ataactaagc aatgtttgtt tggcattttc
      721 gtcaactttt tcagtagtta agacatttac accactgtct ctttgagctt tgacgatttc
      781 tgattctttg ttttcgttga cgatttgagt cgttaattga tcgctataag cgattcctgg
      841 agataagaga ttcattgtag aagaagattt cacgcgtagg atccccgaga tcttcaattc
      901 atcagaagca ttctgataca tcgcatcata atctgtatta gggataaaat tcccagttgg
      961 aagttttgta tagtaagtat tgttgtttac taatttgaat gttgtaccaa cgatatcatc
     1021 aaaatcaagt ttttcgtctt ctttcacatc aaatccaaga ttcttcaatg cgttgatatt
     1081 agtattatta ttgccgtcta cgatcagcac gacatctgtt gctgaggctg gataacttcc
     1141 ggcaagtaaa gaataattgt cttttaaaaa gttttctttg ctagtgtcaa gctgtgtagg
     1201 gaaacttgac actcccacac ctgtcattgc tgacatcgta ctagataaag aaagagattc
     1261 cgtatcagga ttttgattcg agaagctgac aggctgtact ttgccattta catcacgtaa
     1321 aagattgatt cctgtagtac gggtaaatcc gatattatta cttaagttag gatcgatgtc
     1381 tgttacgtaa tcgacgtatg tttgatcaat cttattcgtg tgctgtgctt tatcttcttc
     1441 gctgactttt gcagtaatcg ttttgctgtc tggataatcc gctttgcttg cacccaatcc
     1501 ggcatcgtca cgagtctggt tagtcgttac cttagaaata gtaatcggaa atttcgccat
     1561 cgtttcagct tgcgtgttat cgatttgttt ttggaatcca gaagaaaggg acagaacgat
     1621 cgcgattccg atgatcccaa tactggaagc aaaggaggtc aagaaagtac gtcctttttt
     1681 tgttcggata ttgttgaagg aaagctttaa cgctgtccaa aaactcatct ttgttctgcg
     1741 taagttaaat tgatcatctt ttggtcgttc gatgtgagga tgggaatctg ttaagatctt
     1801 accatcagaa aattcaatga ttcgatctgc gtattgatgg gccagttcag gattatgtgt
     1861 gaccatgatc actaattttt ctttcgataa ctcttggatc aattccatga tctggatact
     1921 tgtttctgta tctaacgctc cggttggttc atcgcacaaa aggatatctg ggtcattggc
     1981 tagtgctcga gcgatggcca ctcgctgcat ttgtccacca gataactgat ttggcttttt
     2041 atgcatatga tttgtcaagc cgactcggtg caatgcatct tctgcccgtt ttcgtttttc
     2101 gtctttagaa acaccactta atgtcatccc aagttcaacg ttttcaataa tgcctagatg
     2161 accaatcaag ttgtaacttt gaaaaatgaa accgatagag ttgtttcggt aggcatccca
     2221 gtcactgtcc ttaaagtctt tagtagactt tccgttgatt accatatcac cggaatcata
     2281 attatctagt ccaccaatca cattcagcat agtcgtttta ccagaaccac taggacctaa
     2341 aatcgctacg aattcttttt gtctgaaagc gacagacaca ccatccaatg ctttggtggt
     2401 ggtttcgccg actttataat atttttttat atcttttaat tgaagcataa tcacccttct
     2461 cccgtattga aatattcggt atactaataa gtatatcaga acaatgatcg tagtatacga
     2521 aataaatgtt aactgaagat gaactacaag aaaaaatcta gaaatttttc aaaaaacaga
     2581 gggagatcaa tgaatcgtat tttagtagta gaagatgatc cgcaattaca gttgctttac
     2641 cgttctgtgt tggaacgagc gggcttttca gtcatcaccg cattgaatgg agaggatgcg
     2701 ctgaaaaaac tagaagcgac acgtatagaa ttaatcatta cagatatcat gatgccgaaa
     2761 atggatggct atgacttgct agactcatta cgtagcacca gatttgacac acctgttcta
     2821 atgattacgg caaaggccga ttttgaggat aagaaacgcg ggttcaaatt aggagcagat
     2881 gattatatga caaaacccat cgatgtcaat gagatgatcc tacgtgtgga agcattgctt
     2941 cgacgttcaa agatcaatta cagcagtagc ttgataatcg gacaaacaaa attagagcta
     3001 gatgcttata cagtgttaca agatcagcat tcgttgattc tgcctcaaaa agaatttctg
     3061 cttctgtata aattactgtc ttatcccaac aagatcttca ctcgccaaca gttgatggat
     3121 gatatttggg gattagatac gaatacagaa gaacgaacga tcgatgtaca tatcaaacgt
     3181 ttaaggaccc gttttgaaaa gacggaagat tttcaaatca tcacagtacg gggactaggc
     3241 tataaggcgg ttatttaaag atgaaaaaaa agctattttc acaattatgg atctattttg
     3301 ctagtattgt cttcatttcg atcttagtga cactgctgtg ttttctcggt tttattttct
     3361 tattgtctcg acattctatc tcgcctgctg aatcggctgg aaaaccaacg ttatacccgt
     3421 tattcgtctt tgcggggttc agtatggtcg tgggtacagg aatatcgatt tttgttgggc
     3481 ggcgtatcct gcatccaatc agtgcgctag ggacaaatat gagtctggta gcaacgggag
     3541 atttttcgat tcgaatggat gaacagcaga aagtagcaga agtacagcaa ctgtataaag
     3601 atttcaacgt tatggtccaa gaactcaata gtatcgagac actgaggaat gactttgtgt
     3661 ccagtgtatc ccatgaattc aaaacaccac tagcaacaat ccaaggatat gttcaattgt
     3721 tacaagcccc aaatctctct gacgaagaac gccaaatatt tttatatcgg attatcgaaa
     3781 gtatcacgca gctttcacaa ttaacagaga acatcttaaa attgaacaaa ctggaaaacc
     3841 agcgtatcca actggaaaag aaagagtatc gattagatga acaaatcaga gaagtaatcg
     3901 ttttttttca gcctaaatgg gagaaagaac agctagaatt ggatatcgaa cttgctgcag
     3961 taaattacac aggcaatgaa gagtttttgt accaagtgtg gctgaatatc atggataacg
     4021 ccataaagta caatcaaatc aatggacaga ttcatatcaa actatttgag acagccacag
     4081 agattgtact ggaagtgacc gattcaggtg taggaatgaa tgaagagacc agagatcgaa
     4141 tgtttgaaaa attttatcaa ggagatacaa gtcgtcaaat ttctgggaat gggctaggac
     4201 tttcactggt taaaaaaatt ctagaactgc atgatggacg tattgattat agcagtatcg
     4261 aaggagtagg aacgacagca atgatacggt taagtaaaca ataggaaaaa gaataaaacc
     4321 cctaaaaatt ctattgaagg tttttagggg tttttagtcg tttttttgtg attagggaaa
     4381 tgttcttacg gttcttttgg cataaaaatc gctgctttct tattgaacta cgagaaatgt
     4441 tagaggtgtc attgaaacaa ggacatgatg caagaaagag agcgaaagtc atgcgtcgct
     4501 ctcttttttt gggtggatag atggacagaa aatgtagggc ttagttttct tcttgattta
     4561 aatactccca aaatgtatct gaagccaaag aatgctcacc gactttacga gtaaatgcaa
     4621 gataactctc tgttgttgga gaaataggga gaatcacagt attttctttt aatagattct
     4681 tgactgtttt ttccataaca atagcaatcc ccatgtcatt tgcaatcata ttgatgatca
     4741 agtcgattct tgctccttta taagagattt ttggttcaaa tccagcttcg cgacacagat
     4801 ctagtacagg ttgatacaag ttcgtagtgg ggccaaggag caaaaagttc tcctttttca
     4861 gttcgcttag atccaatttc tttttactag ctaaaggatg atttttaggt agaactgcta
     4921 caaaatcgtc tgtttcagta ggcaaccact caatcgaact tggtggggcc tggaaacttc
     4981 tagcaaagtg gatcgtgtca ctatgttcat tggcgtcatt gtaagttaat tcgacacttt
     5041 ccacttcttt caactggact gtaaattcag gatggcgttt caaaaactcg gtgattttgg
     5101 aaaaaccttt ataattgatc attgtcggta tcgttaagat atttaatgaa agattgttgt
     5161 ctttcgtata ggtttcaagc acttcttgca tagcatggta ttgttctacg acatttttta
     5221 catatgggag caataagctt ccagcttcgg tcaattcgat ttttctatgg gcgcgttcga
     5281 ataatttgac gtctaattct gtttctaacg aaagaatctg tttagaaata tttccttggg
     5341 tggtgaattg tcgatcagca gttttggtga aattcattgt ttcggctaaa tcaagaaaaa
     5401 cttcgaattt atggatatcc atcatattcc tcctacttat tccaataaag aatactttaa
     5461 cacaaaaaat gaattggtgt aatcttctct gtctgttaca atctttcttg cagcgaaagc
     5521 aataattaca gaaatttttc tgattttttt cgcttcgtag aaaaagagag gagttttttg
     5581 atgccaactg gagttttgat caatgtaggt tccgttttgt ttggcggact aattggcgga
     5641 ttagtaggaa ataaattaag tgaacatttt aaagcacagc tgacgatggt ttttggtgtg
     5701 tgttcgatgg ggatgggaat ctattccatt gcgccgatga aaaatatgcc ggctgttatt
     5761 tttgctttag tgataggaac agcgattggt ttgattgttc atttaggtaa tgggataaat
     5821 aagggggctg cgctgatgca agtacctatt tctaaaatct tccctagtga aaagcttggt
     5881 atgacccacg atgaatttat cagcacatta gtgacggtca ttgttctatt ttgtgcaagt
     5941 ggtacgggga tctatggaag tttagattct gggatgacag gagacagtac gattttgatt
     6001 tctaaatctg tccttgattt tttcacagca gctatttttg cgtgtaattt agggtatgtc
     6061 gtttctgtga tagcaattcc ccaattcatt attttttata ttttgttctt attggcaaaa
     6121 tttatttatc cattgactac accagacatg atcttagatt ttaaagcttg cggcggattc
     6181 ttgatggtcg ctaccggttt tagaatgatc aacgtgaaaa tgttcccagt agctgacatg
     6241 atacctgcaa tgatcgttat catgccactg agctggatgt ggacaaattg gattatgcca
     6301 ctgttataat aaaaatcaaa gagaagccat tttttgctga ctaaaaagga tcaaatcttt
     6361 tagtcagttt ttttgtattt gacaaactga ttttgaaact atattatatg ttgtataata
     6421 tagattgaaa taaaatatta cacttgagaa tagaaggtga cgtaatgcac atccagattc
     6481 caactgttct tcttgatggt actgtgctag cagcattaag caaggaagat atgtatgggt
     6541 atgccttaac aaaaatcgta caagaaagtc tctctgtcag tgaatctaca ctatatccgg
     6601 ttttgagaag attgaaaaaa gaaggatatc ttgagaccta tgatgaacct tttgaaggca
     6661 gaaaccgtcg atattactcg ttgacaccaa tgggaagtcg tcatttagaa gaaattattg
     6721 atgaatggat tgtttttcga gattctttag accggttatt ggaggagagc acaaatgaat
     6781 gaatatttga atgaagttgt ggatcatctt caagaaattc ctgaagacga tcgttttgat
     6841 ttgatccagt attatgagga atatttttta gattctggaa aaacattaga tcagatcatt
     6901 gatgaatatg gtacaccaaa gcagtttgcc ttaaaattaa aaatcagtta cttttcagaa
     6961 gaagaacaag cagaaaacga gttagtgaaa aatccaaaaa gacaaattcg cctgatttgg
     7021 ttaatcatta tcggtttatt tgcttctcct gtcctcattc cactagcact ggggtttata
     7081 ttggttattg ggggactatt ggttgcttta ctagcgctca tttttagtat ctatgtttta
     7141 tgtatcagta tcttaggtgt tggcctattc atgatcatct caggaatcag tgtgtttgga
     7201 caatcgattc tgagtggatt attctttatc ggtgtcggtc tgttcgccac aggaagcgca
     7261 gtatttattt cgccactgtt gctgaaagga acaaaagggc ttttttatgt catgatgaac
     7321 tttgttaaat ggatgggacg acgttttatt acaaaaagag atctgcattc agcgggcgtt
     7381 tgaaggagga cacataaatt gagattgaaa cattttttga ttaccggtat tggtctgatg
     7441 cttactggtg gcctcctagc aggtgcaact tatacattag gcgctcaaaa gagtctgact
     7501 tgggataatg gtcctcggtt gatcgatatg gttactgaga caaaaaaaat caaggatgca
     7561 aacaaaatcg tcatccatgc agaaaatcaa acagtttcgg tcatacgcgg attggatttt
     7621 gagataaaaa cgacttatga taagacagaa aagccagaaa taaaggagaa aaatggcgtg
     7681 ttgactgtga atgcagtcaa aaaatcaaca ggtgcaatca ttagtttaga agacacaagt
     7741 gcacaaatca ctattgttgt ccctgcagat catgaattat ctgaattgac aatcgaaggg
     7801 aataatagtg caattgatat caacgaagta tcaatcaaaa aagtgaatac taagacacat
     7861 gatggctggg tatcgttcaa tgaatttaca ggaggaacaa taactgcgaa agatgaatta
     7921 ggctcacttc gattagatga ggccaatgtg gataacttga tcgtcacagg tgaaaacagc
     7981 aacttccatc tagaagacag tttgatcagc aaagaaggaa aagtcatcct aaacggtggc
     8041 aatttatctt tgcatgagat ggaaaacagt ggctatacta tccaaacgtc tggtgaatct
     8101 tatattgaaa aagactacca gccaataaca aatactttgt tagaacaagg acagaaaaag
     8161 ctaaagatca ctgctaaaga cacatcgatc aatattacga cggatcaaga ggacaatgaa
     8221 acagattgat cctgtactca actggatatt ttaagaaaaa acagcaaaga tccttccaat
     8281 ctttgctgtt tttcatcgct taacgcacat aactatagta aaataagaaa gaaaatatct
     8341 aaaaagaaaa cagggtgaaa gagtatatga aggtagaaga aggtcaaaga gaaaagttaa
     8401 aaacagaaat ggaaagatta catacttata tcactcaact aagccaaacc ttctatgatc
     8461 cagacaaaga aaaagtgatg gttaattacc cgaacaattc agaaggtcgc caattagagc
     8521 aagtgtacca tgaagttttc aaacatttat tgacggtgaa gaaagagctg gattattatt
     8581 ctttaccgat tatcgataca ggaatattga agtatgatgg gaagaaggaa cgatttatct
     8641 ttaaatctgt tcgggaagat ttgcctcttt cagcagggat ggatctagag gtcttggtag
     8701 aagactattt tacagaagaa aagcactggg ttcgaacaaa attggattat ttaccgcagg
     8761 cagccggagg gacacaggca agcggttggt acatcactga agacaaagag ttagaactag
     8821 aaggtgtaat ggcgcgtatt cgaaaaaaaa attaaaaaag taacattaga tatttattga
     8881 aggataaagg agcgcaggaa gtagcatgag agaaaaaaga gcattttata tcaatggtta
     8941 tgcagggatt gtcggattgg tcgtattagc tttagttgga ttgtttttct tctatttagg
     9001 tatgtggcaa gcgaaagttt gggcactttt tttaagtatt tttctttggt tgatcacgtt
     9061 attgttgtta agttcggcaa ctgtagtcag tcctaatcaa gcaaaagtaa ttttgttttt
     9121 tggacaatat ttaggtacga ttcgagaaaa tggctttttc cttacgatac ctttggcaca
     9181 aaaaatgact gtttcgttga aagttcggaa tttcaatagt tctgttttaa aagtcaatga
     9241 tttggatgga aaccccatcg aaatctcagc agtagtagtc tttaaagtcg ttgatacagc
     9301 aaaggcatta ttcgatgtag cgtattatca agattttgta gaaatccaaa gtgagacagc
     9361 cattcggcat atcgctagcc aatacccgta tgatacattc aatgagaatg atttgacgct
     9421 tcgaggaaac acgacggcag tgtctgacga gttgcaaaaa gagctgcaag agcgtttagc
     9481 agtagcaggt gtcgaagtaa tcgaaacacg cttgaaccat ttagcctatg caacagaaat
     9541 cgctagtgcc atgctccaac gtcaacaagc aaaagccatt ttgtctgctc gacaaaccat
     9601 tgttgaagga gctgtcacaa tcactcaaat ggccttagag caaatcgaag atggacaaga
     9661 aatcaatttc accgatgatc ggaaagtcca attgatcaat aatttacttg tctcaatcat
     9721 tacagacaaa ggaacgcaac ctgttatcaa tacaggcgac atgaaagacc gtaagtgaca
     9781 tactttttgt aagataggaa gaaggagttt aggacggatt attgtcttga actctttttt
     9841 tagttgtttt aaagaaaatg acaaaattga aagaagaatg atagttgatt cgaaggcaga
     9901 attgttcgct tttttctata atcagtatat aaagtaagag agaaggggaa aaagtgaaaa
     9961 aacttctgat cggtctactt gttttcatcg ttgtggtact tgcaggggtg aaaatcgcag
    10021 attacgtagt tatgggtgga gaagaatact atgtacaaat tacgacagat ggtacaaaaa
    10081 cagtttcaaa atcagactct ggacaagaaa cagtcaacta tagatatatc ctgcctggct
    10141 atacgaaaga gggtaaggaa aaagaactgg acttcaaggg acaattgact cgaccgcttc
    10201 gtaaaggagc atatttaaaa atcacctgga acaaaaataa aggtgtcact tcgtatgaag
    10261 aagtcgaaaa aaaagacgtg ccgaaaacag cactggaaaa actaattaaa gggtagggaa
    10321 agaaatgaac aaaatagtcg aagtacagaa tgtaacaaaa gtatacggaa aaaacaatga
    10381 gaaaaagacg caagcattaa gcggcatcag cttcgatgta gaaaaaggcg aatttattgg
    10441 gatcatgggc gcttctggtt ctggtaagtc gacacttttg aatattttat ccacattaga
    10501 taaaccaaca gatgggcata tccgaatcaa tcaaaaagat gtgaccacat taaaaggaaa
    10561 tcaactagca gattttcgtg cgaatgagat tggctttatt ttccaagatt tcaatttatt
    10621 agaaagcttg acagcacaag aaaatatcgc tgttcctctt tctttacaag gcgtcagacc
    10681 aaaagaaatc aagcaacgtg tccaaaaaat tgcagaacgt ttatccatct ctcatatttt
    10741 ggaaagctat ccttctgaga tttccggtgg acaaaaacaa cgggtcgccg ccgcacgtgc
    10801 attgatcaca caaccaacga ttctattagg ggatgagcct acaggtgcgc tggattccaa
    10861 aagtgcccgg gatcttttag atacgatgga cgaattgaat acaaaagatc atgtatcgat
    10921 tcttcttgtg acgcatgatc cgttttctgc aagttattgc caacgtattt tattcatcaa
    10981 ggatggcggt atccatcaag aagtgaaacg tggagatcaa agccgtgatt ccttctatcg
    11041 agaaatacta tctattcttg gaaacttgga acagtagggg gagattgact atgctttgga
    11101 aattatcact tactgggatc aagagccgtt taagagacta cttggtgtta ttctcaggat
    11161 tagttatggc atctgctatt ttttacatgt ttgaatcaat ggcaagcaac gaagcttttt
    11221 tgaaaagtaa tacgatcgtt gcggctacag ttatcatttt ccgattcggt tctgttttac
    11281 tagggatcat cacattcgtt tatattttat atgcaaattc ctttttgatg acgatgcggc
    11341 aaaaagacta tgcaatgttt atgatgctgg gagcaaaagg cagaaaaatc gcacagatga
    11401 ttttttctga aacattctta gtagggatcg cagctacttt agtgggttca gctgtcggtg
    11461 taggattgac aagtatcgtc caccgattat tagtggatca gttagatctc aatatttcgc
    11521 attttacacc ttttagtatt cacgggttgt tgatcactgt tttgtttttt gctgttttgt
    11581 ttttattagc tgctgttgtc aatgcttttt cgattgtcaa aaaaccgatt ttagcattga
    11641 ttcgtgcgga tcagacacca acaagaatca agcagcataa attcttgttt ttccttgaag
    11701 tagtactggg aattgctttt ctaggctgcg gttactatat gatgaaacac gtggggacct
    11761 atcaagtctt tggtataggg gtagccttag ttacgatcgt attaggaact tatttcattt
    11821 tccattctgt gatcattttc tttttatcat tactaaagaa atcagaacag atttcattga
    11881 aaaaattgaa caatttcaca ttgtcacagt taaactttcg tattcgtgaa tatacacaaa
    11941 tgctctcaat ggtcgctatg ctgtttgcct tggcactggg cgctttgacg gtaggattag
    12001 gttttaaaaa tgaaattgca gacttgacag aaaacaattc tggatatgat ctagtgatga
    12061 ataatcctcg ggcagaagat cagcaaaaaa tcaaagaact ttcaccaaca ctttctgctt
    12121 cttatactca aaaagaagat gcgggaacga tttactataa tgcaagtgaa tttgcggctc
    12181 aaccattgat catgataaaa catgaagatg gaacaccgcc gaagatcacg aaagaaaaag
    12241 ttcctgctaa aaaaatgaat gaactgactg tacaagagga acttcgaagc tatgaattgc
    12301 cggaacagca agaaaaagaa atcaaggtag tcagtcaatc agaatttgat caactgaact
    12361 tgccacaatc cactttacag ctagttcagg taaaagactt ccaagcaaac ctgaaaccaa
    12421 tcgaaacact tgttgctcaa aataaaatga acaatccatc tttgcaaaaa gtagagtaca
    12481 gcaaccagaa atatgaaatg tatgaagtgt ataatggtct attctctggt tttgaattta
    12541 tgggattctt cctaggttta gctttcttga ccatgcttgc tagctgtttg atgttcaaga
    12601 tcttgtcagg aagcaagagt gacattgctc gttatgaaat gcttgaaaag ataggaacac
    12661 gacgcgggtt gcttaaacaa tcgatccgta gagaagtagg cgtgttattc ttagcaccag
    12721 gaatcttagg tgtagtccat gtcttattcg gattacaaat gtttggtcta ttcatgcaaa
    12781 atccatacaa agatatttgg gttccgttta cgatattctt cgtattgtat ttcatctact
    12841 atgtactgac gacatggtta tatactggaa tcgtattgaa aaagagattg tgaaaaagct
    12901 gtcctgcatc aagtaataag aacagccaag caaaaatagc tcctcatatt tttcgtttgg
    12961 ttgggcttat taccgcagat gcaagctttt gaacaccgtt tgttcggaaa aagaggttgt
    13021 gacaatattt ttgtcacaac ctttttttca cggtctttta ttagaatata gtaaattttt
    13081 tctcgttact cacggaataa gctgttaaga aaacggtttc catgtatact ggaatcagtg
    13141 caaatgaaag aaaagagggt gcaaggaaca tggaaatcaa tgaacaaaat tttggcgaaa
    13201 actatcactt gattacgatc acgaatcaaa atgggacatc tttgtctgtc agtgatcttg
    13261 gggcacgggt tgttcatttt caaacaataa tcgataatga aaaacgagag ttgatcttag
    13321 gttttgatga agcaaaagaa tatctagaaa aagaccctta tatcggtgct tcaatcggac
    13381 gtatggctgg acggattacg aatggcacat tcaaattaga tgatgaaacg ttcgaagtaa
    13441 aaacagaccc ggacactggt cattgtttac atggtggtgc acctagcttt gaagccaagc
    13501 agtggcatta tacagtcatc aatggtgaaa acgaagcatc ggtcattttc tatcagacta
    13561 gcccagatat ggaaaacgga tttcctggaa ctttagatgt agaagtacgg tatacgctga
    13621 cgaacgatga tacatggcga gtgacggtcc aagggatcag tgaccgcaaa acagtattca
    13681 atcctacaaa tcatgtctat ttcaatttaa ccggtgatcc agcacagtca atcgacgaac
    13741 atatactttg gttgaatagc cgattatttg ctacgctcaa taaagataca acgcctacag
    13801 gagaagtagt ttcagtaagt ggcacagcat ttgacttcca acgtcctaaa aaattaggtg
    13861 aggtctttgc cagtgatttt cgccaatcaa tgatagtggg cggcatcgat catccatttt
    13921 tgttgaaaaa acaatctggt ttactaaatg ctgcggaatt gacaagccca gatgaaaaag
    13981 tatcgatcca agtaaaaaca gatgcttcag gaatcgtgat ttttacagca aattttggag
    14041 aagatggacc ggaaatgcgc gggaataaat tagtaaatca tggcggtatc acatttgaga
    14101 ctcaagaact gccaggagcg gagcgacttg aaagttttgg agatgtcact ctggaaccta
    14161 accaagtacg cgagtttgta acggaataca aaataaatat aaagaaatag aataaattaa
    14221 aaagggagtg tgacagtgtc acatcccctt tttaatttat tctatttctt taaaggcaga
    14281 tcaagccaga atgatttgtc taaacttaac aaggttagta caggaacagt tgcagcttta
    14341 actgtacgag tagttgtttc tggtatctgt cttgccgtaa aattgttcca caaatcccag
    14401 attgaagcga cacggaagat ggaagaagcg ttgttgacgc gttttatcgt tgaaaattct
    14461 aatcctgtga ttggcacgcc atctgttgtc catttgattg gcacatcgaa tcgcttagac
    14521 atattgagga tttcagcgtt tgcttcttca gttgttgctg cttctttttt cggttcatca
    14581 tattttgtca ggtcgtacgc ttcgatgatc gcgttcaatt tttgtgaata ttggcgatct
    14641 gtagcataac gtccttccaa atatttggtt gcttctttgt agtcttttgt ttccgacttc
    14701 caagttggtt tgtagaaatc aggattgcca gtcagtccat ttttgatcaa gtcggcatag
    14761 tcttccaaac tttccttgta agaaggatat ttacggaatt gtgcgcggat ttggtagctt
    14821 tgtccggaac tatcttgttc aagtgtattg aaggtcacgc tgcttccttt atatgatcct
    14881 ttgataccaa aaaggttatg atttggttcg cttgataata agctgtttcc agatccactt
    14941 tcaaggatag cttgagccat catgactgat gcgtagattc cttctttttg cccgatttcc
    15001 tgtgcatcat ctgcaatctt tttcaagaac tgatcagttg tttggttttg actgaaatgg
    15061 aacgagccat ctaatccatc taaaatagat gagccgcctg tcagcgaacc tgaagcagca
    15121 ggtaaacctg ctaagtttac tcggacagca aaatcaggcg tgaaatctga aatagaagtt
    15181 actcgaactt tatctcctgg ttgaggcgca tggataaatt gtccgttccc aatatagata
    15241 cctacatggt gagtaacacc ggaagaaggt ccccagaaga cgaggtcgcc aggttgggcc
    15301 tgacttactg gaatctgcgt acctgcatat tgctgttcgc ctgtccagcc accgatataa
    15361 tgaccggttg ctttcatata tacgtaataa gtcaaaccag aacagtcaaa agtatttgga
    15421 ccttttgctc cccatacata tggttttcca agatgcttga atgcttctcg aacgattgct
    15481 tgttggacat tcgttgtatt tgtaggcaat acagcatcaa ttggtgattt tacaacaaaa
    15541 tcagaagaag cagaagatgg attctctata ccgctttgat ttgctgtatt tggacgagat
    15601 gaagcattgt tcgttccgcc attcgatgaa ccattgtttg gatggtttgg ttctctctga
    15661 gtatttccat tgttattcga tccagatggg tttgtttgat tcgaagaatt cccgctgttc
    15721 gaattattgt tattattgtt tgaattattt gaaccattat tattgttgtt attacccaca
    15781 tctggtttgt ttggcgtttc cggatctggt gtactaggga cttcttcact aggatcagtt
    15841 ggtttctctg ttgaagtatc cggtccagct ttaaattctg cagtaataga taaattgttc
    15901 gtacctactg taaaggtctt tgcatcataa tttacttctt tggcaccatt aacagcgact
    15961 gaagcaattt gttcaccttc agctggcgta atagtgtaag ttaccttcgt ttccgctaat
    16021 aaaccttcta ccgttccact ttccgtaata tccaaaggtt gctgttcagg ggaatccgct
    16081 gtcgaagcag tcagttggat cttaccaatc aaagaaggag caattgtaag ggaatatgtc
    16141 tcggctggct tttcattttc tgatggatca gccggatcaa tttcagttgg ctcggtcgat
    16201 cctgttgttg agttagttgt tccagttggg tcttctgtaa aagaatccgt tgtttcacta
    16261 gtagctgagc tggatgtagt cgtatctgtt gtgactgttt gatcagctat ttgattatct
    16321 acggcaaata cggtattggt cgacaaaaca gacggagcaa tcagccaagt agcagctagc
    16381 aaacgtatcg tttttttatt tttcataata aactagtcct tttcctttca ttgattttct
    16441 gtcttattca gcattcatta gacatttttt ggaacagata atcgtgtcga aaagcttttt
    16501 cttttcataa atctgtctgt gtattctaaa taaaacaatt tgtaaataag caacgagcat
    16561 tcattcgttt ttctttgacg ttcattttat tgcatagacc acctttgttt ggggataaac
    16621 aagtggggat aaaaatggtt atgcatatgt aaattatacc aaagtttgga aagtatcaca
    16681 aaaatttata aaaataatga tcctaaaaag aaaaagactg aaatatgtga taaacatctc
    16741 atacttcagt ctttttcatt aactattcat aagacattag gtcttcatca aacccgctaa
    16801 cgagagtagt gatcattgct acgactagac taacagccag tcctgctaag taccagaaaa
    16861 aagtaccgga aatgaatgca ggcaatgtgg tcagactacc aaatacaaaa gcgatcgctt
    16921 tcacttgggc aaatcccatt acagctccac cagcagctgc accaatgcaa gcagcaacaa
    16981 atggtttttt caatgggatc gcaattccgt aaacagttgg ttcggtcact cccgcaagta
    17041 aaatcgaagc aaaagcagaa ccggagatcg aacgggtttt tgaacgttta cttcgcagaa
    17101 atacacctaa agctgcacca gccattccaa aattggctgc tcctgtcaac ggaacgattc
    17161 ggtcgaaccc ataagcagag atattactga tcatgacagg gtttacagcc cattggatac
    17221 caaatattac caaaatattc caaccaccag cgatgattgc gccactgatc ggtccgttaa
    17281 tatccatcat ccaagtaatt gcggcagcta aaccttcgcc aaggtaaaca cccgcaggcc
    17341 cgaataccat taacgtcaaa ggaaccgtga taactaatga aagtaaaggt acaaatacta
    17401 attgcatatt ttcgcgaata tattttttga ggaatttttc aacaaaagaa taaaagaaaa
    17461 tacctaaaat cgccggcatg acagtagacg tatactgcat cagtactaca ggaatcccca
    17521 gaaaatcagt agctgctttg ccactgtctg aaagtaaaga ggtcagattc ggttcaatca
    17581 gtgctgcagc aatgacagcc gcaatatatc cattcacttt aaacgttttg gcagcagaaa
    17641 aagcaagaaa gattggcaga aaataaaaga cggcattcga cgcagcagac aaaatatgat
    17701 aagttccgct tgtttcacta agccagccaa tcgtagtcgc taaaacaacc agacctttta
    17761 atataccaga accggcaaac agcggaatga tcggcgtaaa gatttccgat atcaaaccaa
    17821 atagtttttc gatcggattt agcttttctt tcggagctga atgatcgatt tgacgcgtca
    17881 attccttgta agcaggagcc aatccctgat ccatgattat ttctgcatat ctatcgagta
    17941 aaatgaaata cttaatggaa gggatttgct gttttaattt ttctgcattg gctactggct
    18001 gttttttcaa tttgatacga agccgagtcg cacatggaag gacagaatca atattgtctt
    18061 ttcctcccaa ctgttcaatc agcatatgaa tctgctcgga cagtaccatc tgttcaggat
    18121 tcattgtcgt ttccattaga agaccactcc actcactaat ataatattgg cgtaaggtgt
    18181 catttcgcct gtacgaataa atgcatgaca gttattcaac tcttttttca tttcttcatg
    18241 tgggataaag ctaatcggtg tatctggtaa caataatttg attttttcca atatttccgg
    18301 gttcattgtc tttatttctt ccgccaattc aatcgattgt acctcaagtt cttctaaaac
    18361 gttttctaaa acagataaaa aacttggaag accgtttgag acagccaaat ctattttttc
    18421 agtaccaaag gggacaggca tgcctgcatc cccgatactt aatttatcaa agtgccccat
    18481 ttgtgcgatt actcgtgaaa tatctgaatt gattactttt ccttttttca tgtttgtttc
    18541 ttcctctcat tgttaatttt agactgctga ttgttctaaa gcttcgcggt aagggataga
    18601 aggttgcgca ccataagact gcacagcaac agaagaggct agatttccat aaaggatcgc
    18661 ttctttcaag ttactaaggt ctggctgaag tgtgctgctt aatgcgccaa taaatgtatc
    18721 acctgcggct gttgtgtcaa cggcatccac tttaaaagca gggacgatac cgtgttcttt
    18781 ttctgtatga tagaaagcgc cggcactgcc tagtgtaatg attactgttc caatacctaa
    18841 ttcatggagt ttttcggctg ctgcgaccaa actgttatga tctgtcactc tgattcctgt
    18901 aatgatttct gtttctgttt catttggtat aatgatatct gtcttttcaa gtagttcagt
    18961 aggaatcgtt tcacgagctg gtgcaggatt caggatcgtt gtttttcctg cgtcttttgc
    19021 aatagaaaaa gctaggatcg tcgcatctaa gctactttca aactgagcaa taataaagtc
    19081 actgtttgta atcagttgct tgttttttag gacttcttgt tcatggaaag cattatttgc
    19141 ccctgcatgg atcaagatac tattttctcc tgctgcgtct accataatca ttgcttgtcc
    19201 ggttgtggcg ccttggattt cagctatccc agatgtatcg atgttttctt ttgtcagtag
    19261 atctaataac tgttgtcctt cgctgtcagc cccaacgcct ccaataaaag atgtccgtgc
    19321 gcctgaacgt ttggctgcca ctgcttggtt ggcaccttta cctccgccag cgtgaaaaat
    19381 ctcatgcgca tgcatcgttt cgcctggctt gggcatgttg gttaaacgaa gggtagtgtc
    19441 catattaata cttccaatta ctgtaaccgt gttcatttta caaacctcca actatctcat
    19501 ttattttgat gggaaaatta ttttttaaaa cgttttagta aaacgtttta atagttcgat
    19561 aaaaatatag cacaccttga aagctatttc aagggtgcag ttgaaaatct tttttctaat
    19621 cgtacaggta atcttttttc ttcccattct ttttctggga attggatccg atctaacaat
    19681 aacttggcag atgtttgtcc caattcgaag attggttgtg cgatcgttgt cagtttcggc
    19741 ttgatatact cgcacatatc tatattatca tagccaataa tgctgtagtc ttcagggatg
    19801 ctttttccag cttcctctaa tccgcgatat aatccaaaag ctaattcatc atttaaagca
    19861 aacacaccag ttgcagcaga atcaagaagt tctgctgtga tctggtagcc accttgtttt
    19921 gagaactgtg taggtaagag gatcagttgg tcgtgaggga tttggtaaac atcaagagca
    19981 gatttaaacc cctcaatccg agcgtggaca ttttccggtg gattttcagg atagacaagg
    20041 gcgatcgttt gatgtcctag actaagcaag tgcatgcccg ctaggtaacc accgcgaaaa
    20101 tcgtctgttc gtacggcatc actaaatcct tcagattttt tctgatccaa aacaatgaag
    20161 ggccggcctt gttttttcaa attttcgttg attgcatctg tagagacagc agaagtcgcg
    20221 ataatgaatc catccacacc tcgacgtgtg agctcggcta gatattcgat ttctttttga
    20281 tgatcagaat cagcattaca taatatcgtg acgaaattct gcttatacaa gatatcctca
    20341 attcctcgca tcagtgtact gaagaatgga ttcgtgatat caggaacgag tacaccaatt
    20401 gttttcgttt ccttggtaat catttgtcta gcaaaatagt caggctgata gcctaattct
    20461 tctttggcag ctaaaacttt ctgaacagtt ttcggactga atttctccgt atttccattt
    20521 aatatcaagg agacagttgt gattgaaacg ccggaaagtt tagcgacttc tttgattgtg
    20581 atcttttttt tcaccatgtt catactccta tctaaatcct gtggttattt tcccatgaaa
    20641 tcaaaaagaa gtaaatgaaa atcacgtgtg ccagtactta tttgctttgt tggatagctt
    20701 cgattatttg tttcgttgag cgtactcgtg cagttttcgg aaatatcttt tttatgaaca
    20761 attgatggga ttctttgtca cgatcactgc aagcatcttc agcaagcacg atatggtacc
    20821 cgtgttgaaa ggcatctaat gcagtagcat atacaccgtt tgttgtcgct acaccgctta
    20881 aaatgatagt ttcgattcca cgccggcgaa gttgaagatc aagagaagta ccaaaaaaag
    20941 cacttggatt gtattttgtg atagtcaaca tattatctgt atctttcaaa gaatccttca
    21001 aaagtaaatc agtgaattct ggaggtacag aagcagtcat tccacccata tcagtttgtt
    21061 gactcaaata agggaaagtg gagatgtcta cgttcactaa aactttcaag gcagcggtac
    21121 gtttcaatgt tcttactaac aaatcattgt ttgtcagaat agatgctgca gtgtgtggag
    21181 agagtgtacc gttatttacg atctcttttt gcatgtcgat cgcgacaaaa gcagtttttg
    21241 ataaatcaaa aatcatattt tcctttaaat cattcatgat gttccttctt tcaaataaca
    21301 atgtttttta tgtaagtaaa tcataaatcg tttatttgtc aactaaatta aaggattcct
    21361 ttctttagtc tagagaaatt cagtggtaaa ataaataagt gtgagaaaac gcattcgatg
    21421 catagatata gcgagaaata tcgtgaaaat ggaggaatca gagtgaagta tgtcgaagta
    21481 aaaaatgaat acaaacgata caaaatggga gaaacgacca ttacagcaaa tgacgatatt
    21541 tctttttcaa ttgaaaaagg cgaattagtg attatacttg gtccaagtgg agcaggaaag
    21601 tcaacgattc ttaatatttt aggtgggatg gatacacctg atgaaggtca gatcattatt
    21661 gatgacacag atattgcgca attttcagat aaacagctta ctgcttaccg acgaacagat
    21721 gtagggttcg ttttccaatt ctacaatctc gttcctaatc taacagcaaa agaaaatgtg
    21781 gaattggcaa cggaagtttc tcctgatgcg cttgatcctg tagaggtatt gaggcaagtg
    21841 ggcctagctc atcgattgaa taactttccg tcccaattat caggaggcga acagcagagg
    21901 gtttcaattg ctcgtgcact agctaaaaat cccaaacttc ttttatgtga tgaaccgaca
    21961 ggcgcgctgg attttgaaac gggcaagcaa gtattgaaat tattgcaaaa cgctagcaga
    22021 caacacggca ataccgtttt gatcattaca cataattctg ctttagcgcc catcgctgat
    22081 agagtcattc acattaatga tgctaaagta aggtctgttg aattaaatga tcatccttca
    22141 tccattgatg agattgtatg gtagggggat tttatgaaaa caagaacata tcttaaagca
    22201 ggttttcgcg aaattcggca atctaaagga cgattcattg caatcatcct tattgtcatg
    22261 ctaggaacac ttttatttgt aggtgtcaaa actgctggac ctgtcatgca aaaaacgatg
    22321 gatcactatg ttggtacagc aggactttct gatttgcaaa tcgtttctac tggtggcttg
    22381 acacaaaaag atattgctca agcagaaaag atcccagatg caaaagtaga aacaggaaaa
    22441 cagatctatt attccaatac tggcaaaaac gaagtgattc aaatattttc ttataacaaa
    22501 aataacaagc agaacaagct acaattaact gatggaaaac tcccggaaaa accaaaccag
    22561 cttgtattgg atgaaaaagc ggaagatgaa gggtataaaa tcggtgatat ttatcagatt
    22621 gattcagatg aattgaaaga aaaagagtat accatcacgg gctttgtccg ttctccattg
    22681 ttcatcaaca atttggagag aggatacgcg aacgtcggaa atggtagcgt cgattatttt
    22741 gtctatttac ctgaatccgt ttttaaaaca gatatttatt ctgtcttata tcttgatttt
    22801 acaaacgtaa agaatttgaa tacgtacagc aaagcttaca aagataaaat ggagcaaaat
    22861 caagaaaagg cagaaaagta tttacaagat cgacccgaag aacgcttaga agagctgaaa
    22921 aaatcggcca acgaagaatt agaaccggca cagcaaaaaa tagcggatgg caaagcacaa
    22981 acaacgcaag caagaacacg gttagaagat gctaaaaaac agttagcgca acagtcagca
    23041 actatccagc agcttcctga agcgcaacga acaatcgctg ttcaaacatt atctcaacaa
    23101 gaagctcaac ttgctgaaca agaaaggcaa ttagttgaga aagaacgtga actggcctct
    23161 gcagaggaag aattgaatcg aaatgcagaa aaaattgaaa atttaactaa accatcatac
    23221 ctatacaatg aacgttcaga aaatgtcggt tttcaagaat ttggtgatct gtctgatcgt
    23281 atcgctgcga ttgccaatgt ttttcctgtt ttctttttct ttattgccgc attgatcacc
    23341 tttacgacaa tgacacgtat ggtagaagaa aaccgcaggg aaatcgggac attaaaggcg
    23401 ttaggttata cgaaagttga aatcgcaaaa aaatatgcaa tctatgcaag tctagcctca
    23461 gggataggta ttgttttagg aacgatatta ggaacgaatc ttttgccaag gatcattttt
    23521 gagctgtcta acgagcggta tgatattggg agtgcgctta ttttttatga ctggccgccg
    23581 attttacaag cagctgccgc attcttcatt gccgcatttg gcgctgcaat gatcgtttta
    23641 ttcaaagatt taagagaacg gcctgctgca ttgcttcaac cgaaagcacc gaagccaggg
    23701 aaacgcattt ttcttgaata catcactccg ctgtggtcac gccttagctt taatcaaaaa
    23761 atcagttacc gaaatctctt ccgatataag tcaagaatgc ttatggcgat tatcggaatt
    23821 gctggatgtg ccgggctgat ggttgcaggc gtgggattga aagattctct aagttctgtt
    23881 tcagccaaac aatttggacc gatcattgac tatcaagcaa tcgttacatt ggattcatcc
    23941 acggaagata gtcgtcaaaa agcagcggat atgctgcaga agcaagaaaa agtaaatgag
    24001 atcctagcta ttcatactca aaccgtagaa ttgagaaaaa aaggacatgc ttcgcaaagt
    24061 ctgacagtga tcgtaccaag taaaacagct gatttggatc gttttatcca tttaactgat
    24121 cttgaagaga acccacgctc cttgcctaaa acaggaatcg ctattacgca aaaagtagca
    24181 gaactatttg acttatctac aggagatcat atttcgcttt atgatgaaga tcaaaaagaa
    24241 tggaaagtcg aagtaaaaga aatcgtgaaa aactatctag gtagctttat ttatatgtcc
    24301 cctgaatatt atgcttcagt tactgatcaa tcgatgatgg ataatgcatt tttgctgcgt
    24361 acagataaga tgacaacaaa agaagaagaa atgctttccg agaaactgct tgccacagac
    24421 gctgtgacaa atacgtcttt catgtccaaa caaatcgaaa cgcaagaaga atcaatggcg
    24481 aacttagact cggttgtctt aatctttgtc gtattatcag gactgctggc ttttatcgtc
    24541 ttgtataatc taactaatat caatatttcc gagcgtgtac gcgaattatc tacaataaaa
    24601 gtccttggat tttttgataa agaagtcacg atgtacattg ttcgagaaaa tatcatcttt
    24661 actcttttag gaatcatcgg aggatttggt gtaggatata tattgactga cttcatcttg
    24721 caacaagcgt ctatggagaa tgtcatcttt ccattagtga tcacatggtg ggcttatgtc
    24781 ttatcagcag gattaactat cgtatttacg gttattgtga tgattgttac ccatttcaaa
    24841 ttaaagcata tcgatatgat tgatgcgttg aaatcgaatg aataaaaaca gaggttgtga
    24901 aatcagcggt ttgacctgag tattagagaa acaaacggca aaatagtttt tcatattttg
    24961 ccgttttttg agctaatacc gaaggacagc tgattgaaca ccatgaatca gaggttgtaa
    25021 aaaagccgtc ctgcatcgag caaaaagaat actcaaataa aattagctcc tcatgtttta
    25081 catttgagaa gactgattgc cgtagataaa agcttttgaa caccatttat agaagaaaca
    25141 agaatatttt gctattttaa acaagaggat agacaaatac atgtctgatc tcttgttttt
    25201 tattgggcaa cagacctctt agaatgtata aagataaatt ttacaaacgc tttcggttca
    25261 gttataataa taaatgataa cttgtatata taagttgtgg taggtaataa atcagttgaa
    25321 tggaggtaaa tatccatgac atttaaagac aaagtcattt accaaatcta tcccaaatct
    25381 tacaaagaca gtaatggtga tggaattgga gatttaaaag gaatcaaaga aaaactcccg
    25441 tatctgaatg atctaggcat cgacatgatt tggctaaacc ctatttatcc aagccctcaa
    25501 aaagacaacg gttatgatat ctctgattac acagccatcg atcctatctt tggtacgatg
    25561 gaggaattcg aacagctaat agccgaagca aagaaatacc ggatagaaat catgctcgat
    25621 atggtgctga atcacgtttc gacagaacat gaatggttca aaaaagcact agcaggggac
    25681 cagcattatc aggacttctt cattttaaga gaagagccaa cggattgggt atcaaaattt
    25741 ggtggaagtg cttggtcgcc atttggagat acggggaaat attatttgca cttatatgat
    25801 cggacacaag ctgacttgaa ctggcggaac caagcagtaa gagaggaact attcaaagtt
    25861 gttcgttttt ggatggaaaa aggggtaaaa ggattccgtt tcgatgttat caatgttatc
    25921 ggtaaagacg aacaattgaa aaataataaa gaaaatgatg gcaaacctga atatacggac
    25981 aaaccaatca cccatacgta tctaaaagaa ttgaaccaag caacgtttgg gacggattcc
    26041 actatcatca cagtcggtga aatgagctct acgacgatcg aaaattgtat cttgtataca
    26101 gaacctagta gagaagaatt atcaatggtc tttaatttcc atcatttgaa agtcgattat
    26161 gaaaatggga ataaatggtc tactcctcca tttgattttg cagaattaaa gcgactattt
    26221 catacatggg gagaaaagat gagtgagggt aacggctgga atgctctatt ttttaataat
    26281 catgatcagc cacgtgcgct caatcgattt gtagctatcg atcagtatcg aaaacaagga
    26341 gcagagatgc tagcaaccat gatccatttg aaccgtggga cgccctatat ttatatggga
    26401 gaagaaatcg gcatgatcga cccagactat tcatcgattt ccgactatat tgacgtagaa
    26461 agtttgaatg cttataagtt actccttgaa caaggattca cagaaaaaga agcgtttcgt
    26521 cggatcaaag caaaatctcg cgataactct cgaacaccca tgcaatggtc agacacagaa
    26581 caagcaggat ttactacagg gaaaccttgg ttaaaagttg gaggcaagct agaagagatt
    26641 aatgtagaaa aagaactatc ttctgaagat tctattttgt cctattacaa gaaattgatt
    26701 cggctgagaa aaacatatcc aatcgttgca caaggagatt atcatgctta tgaagcaaat
    26761 catccggaag tttatggctt tcttcgccaa tttgaaggac agcagctgct tgtattaaca
    26821 aatttctatg ataaagatac aaggatcgcc atcccagaag gattcacaga agccaatatt
    26881 cttatcgata attattccga tactgttctt catcaaagtc tggagctaaa accataccag
    26941 gcaattgctt tgctgaaaaa ttaaaacaaa aacacaaaac gtcgttccta cgagctattc
    27001 tcgtaagatc gacgttttgt gttttattta aaataatctt cttcagaaaa tgaagagttt
    27061 tcatactctc ctttatcgcc ataaaattta ttgtagcgtt gtttgaattt acggaaaata
    27121 aaagtaacga gtaatttgac taacgtatag ataggaatac caaaaataac gcccacaatt
    27181 cccattagat cacccatgac aagtaaaacg attaagatcg taatcggatg gatctgtaaa
    27241 cgatcaccca ttactcgagg aatcaccaag tcaccgtgga gaagttgtac ggcaaaccag
    27301 acaatgacaa atttgacagc cataaaagta gagtcttgga atgcaatgat caaacctgga
    27361 aagaaggcga taaacggccc aatatatgga atgatacata acaacccagc agaaatagct
    27421 aaaacactag caaattctaa tccgatcagt aagaaacaaa cccagtagac tgctccaagg
    27481 ataaatgaag caatgatttg cccctttaga aatgaaccca attgttgatt agcaatcttt
    27541 gtcaaatttt taaaatctgt acgaaatgct ggtgggacaa tcgctaaaac tgactgataa
    27601 aatttttgag gatctctcaa aaggaaaaaa gcgacaattg gaccagtaaa taaagtaata
    27661 aaaatagctg taacagttga aaaaatgttg ctgatccgtt gtgcgccact ttcccaataa
    27721 tttccaataa atccagctat atcagacgtc acgtcgtcaa tagaagcgaa aaattgatta
    27781 aatgaatcgg caaaaggtgt tttctccaaa aattgtcgga gactctcttg aaaatcttga
    27841 aacaaatctg gcaaactact gatcaatgtc tttgcctgtt cttcaagaaa aggaataagc
    27901 catatgcttc caagccccaa taaaaataaa ataatcacat aaacgataat gactgacaag
    27961 ttcctcgaaa ggtgcttgct caaacgtttg atgactggtt ccaaaagata atacaagatc
    28021 agtgcaaaca ataatggggg caagatagta gcgataatga ttccgacagg cttgaatata
    28081 aacgagatct gatggaaaaa ataaatgatc agcgcaagta agataactat tcctaaaaca
    28141 tagtaagagg tttttcctcc taaaaagcgg atcaaacgat tcgttgtatc tgtttctttt
    28201 ttcactcttt ttcctccttt atctataact tcattataga caaaagcggg tataaaagga
    28261 aaatgataga ggttgattgc aaaaatatcg tcaatcattc atataaactt tctcatcaat
    28321 taaaataaga aaacgcttga cagttctttt aaccatatga taaattaaat cctgttgcta
    28381 aaacgtaact attacggttt gcgaaaagcg aatcggaaca ttcaaatgaa agaagatgag
    28441 aagaggagtg tcataaatga aacgattgat aggtatattg gccatgctgg ttttagcagg
    28501 tatgttaact gcttgcgggg caagtgggaa agccgaggac agtaaagaaa aattgagcgt
    28561 gatgacgacc ttttatccca tgtatgattt tacaaaagca atcgtcgggg atgaaggaga
    28621 agtagagtta ttgattccag cagggactga ttcccacgat tatgaacctt ctgctaaaga
    28681 catggcaaag atccaagata cagatatctt tgtttataat gatgaaaaca tggaaacatg
    28741 ggtccccgca atccaaaaaa cattacaaga aggcaatgtc catacgataa aagcgacaga
    28801 aggtatgctt ttacttccgg gaagtgaaga aggacatgat catgaccatg aacacgggga
    28861 agaaggccat acacatgagc tagacccaca tgtatggcta gctccaagtt tagcaatcaa
    28921 gcaggtagca aacatacgtg atcagctgat tgaagcttac ccagaaaaac aggaagtatg
    28981 gacaaaaaat gcagcagcct ataccgagaa acttcaagca ctccatcagc tttatcaaga
    29041 aacctttaag caagcaaaac aaagatcttt tgtcacgcaa catgctgcat tcaactatct
    29101 agcgttagaa tatggattga atcaagtatc gattgctggt ctttcttcta gtgaagaacc
    29161 gtctgcggcg agaattgctg aactgaaaca ctttgttaaa gaacatggaa tcaactatat
    29221 ttattttgaa gaaaacgcca aagatagtat tgccagaacg ttagcaaacg aagcaggtgt
    29281 atctttagaa gtattgaatc ctttggaagg attgacgaat gaacaaatag aaaatggaga
    29341 aaactatctt tctatcatgg aagcaaacct cgaagcgttg aaaaaaacga cagagacaga
    29401 aaacccgcta gaagatactt tgacccccaa aaaagaaaag agcgtctaca acggttattt
    29461 tgaagacgag gatgtacaag atcgctctct ttcagattgg gcaggaactt ggcaaacagt
    29521 agatacgtat gtagaagatg ggacttttga tcctgttttt gaatataaag aaaaattgaa
    29581 tcaggataag acagcacaag aatataaaga atattatacg aagggctatc aaacagatat
    29641 tgcttcaatc aagatcagtg agaaagagat ggcatttacg tttaagaatg gaacaacgaa
    29701 acgttcaaat tatcgttatg tagggaaaga aatcttgacc tacgaggcag gaaaccgtgg
    29761 cgtaaggtat ctattcgaag cagaagataa ggaaagtggc gcttttagat atattcaatt
    29821 cagtgatcac acgattgcac cggctgactc cgaacatttt catttatatg caggaaatga
    29881 aagtcaagaa gtattgctga aagaaatgga gaattggcca acattttatc cagatacaat
    29941 ggatggacgt gaaattgcac aggaaatgct tttgcactga taggcaaaaa agaaccgaag
    30001 ggtttggatc ggctggtcat ttagtcggtc caaactattc ggttcttttt atgaaatcaa
    30061 tttctttaat ccacggatag aggctgggat ggtctcgccg tttgccaacg tgacttctct
    30121 ggttttttta ttaatcgacc gcacattttg tgtattgata acataggatt tatgacaacg
    30181 gataaaattc cgatgcattt tctcgatttc tttgatcttc tcatagaatt gtaattgggc
    30241 atgagtcaaa tggacttcca gtttatgtgg cgctcctagc gaagtagaaa aatattggat
    30301 gtctttgatc ggtaaaaatt tgatctcatc attaaactct attttgaaga tatctttttg
    30361 gatttgggac atttgaaaat cttcgaaaat tttgctcagg atatgctcga cattttcttt
    30421 taaatcataa agatcttctt tgacgatata atcagtcggt tcgatgttgc tttttaagat
    30481 tttcatcgac atttccgtat gggaagtcac atagattatt tttcctaaag gatctaattc
    30541 acggatcaat ttaccgatcg ctacaccatc catttcactg tcttctagtt cgatatctaa
    30601 aaagtaaata ccaaaagaag aagttctgat cttggctgca ttgagaagtt cttccggatc
    30661 agaggtagaa atatggacat aactgtctaa gttttcaatc atgattctat ttttgatcac
    30721 cgttgataaa acttctaatt gttttggatc gtcttcacaa atataaatgg gaatcatatc
    30781 attactcctt tattttattt gtatataaat cgtttgagaa aaacgctggt tttcaattga
    30841 tgtttcaagg aaaagataag gattggtctt tgtcaattca tccaagataa acaagccaag
    30901 gccttcgttt tttggatttg tttttgttga gaaccctttt tgtttcaact ggtttaacgg
    30961 taatgtattt gttgttgtat tgatcacacg aatcatcacg gaatgattct tcttgagtat
    31021 ggtcaattcg atttttttct tctttgcagc actagctcct tcaatcgcat tgtccaagat
    31081 aataccaagc atacgtacaa ggtttaccgt atgtttcgta tccagttcga ttggctcagg
    31141 aaaatgaagc tggacgatca accctttttc ctgtgccatc atgagtttta atgacagtaa
    31201 actacggatc tcaggaactt tcatattatc caacatactt agattggccg gaaaaaggtt
    31261 catcatctgg cgtgtaggta atatcgtttg atcataataa gaccgtaatc cttctaagtc
    31321 gccttctgaa atatagctgt gaagagaaaa aagcagattt tggtaatcat gtttgaattc
    31381 tctcaagtct tgatagtttt tttctagatc tttgatataa tcctgttgca tcctgacttg
    31441 ttgctctaat aactgatcag ctaatttctt attagagatt ttccaataat aaaccaagat
    31501 gatcaggcca acacacagat atagaatgaa aaaactcgta ttcaaagcta agacatcagg
    31561 tgtttcacca gaaaaacggg tataaaaaat aatgat
//
LOCUS       DACGLW010000027        29572 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.027, whole genome shotgun sequence.
ACCESSION   DACGLW010000027 DACGLW010000000
VERSION     DACGLW010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.027"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            complement(47..1342)
                     /gene="purB"
                     /locus_tag="IXT53_001246"
     CDS             complement(47..1342)
                     /gene="purB"
                     /locus_tag="IXT53_001246"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HAQ4905087.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1355..2488)
                     /gene="purK"
                     /locus_tag="IXT53_001247"
     CDS             complement(1355..2488)
                     /gene="purK"
                     /locus_tag="IXT53_001247"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HAQ4905088.1"
                     /translation="MINLVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVS
                     DCPAAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTD
                     LLAITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKG
                     QYVLKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHN
                     NILHETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRP
                     HNSGHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENK
                     PDWQFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2472..2960)
                     /gene="purE"
                     /locus_tag="IXT53_001248"
     CDS             complement(2472..2960)
                     /gene="purE"
                     /locus_tag="IXT53_001248"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HAQ4905089.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3376..4683)
                     /locus_tag="IXT53_001249"
     CDS             complement(3376..4683)
                     /locus_tag="IXT53_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HAQ4905090.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(4686..5267)
                     /locus_tag="IXT53_001250"
     CDS             complement(4686..5267)
                     /locus_tag="IXT53_001250"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HAQ4905091.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5383..5480)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(5839..7131)
                     /locus_tag="IXT53_001251"
     CDS             complement(5839..7131)
                     /locus_tag="IXT53_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ4905092.1"
                     /translation="MKKKYVQVGLGGRARFFYETIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7128..7751)
                     /locus_tag="IXT53_001252"
     CDS             complement(7128..7751)
                     /locus_tag="IXT53_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905093.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(7754..8590)
                     /locus_tag="IXT53_001253"
     CDS             complement(7754..8590)
                     /locus_tag="IXT53_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ4905094.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(8607..9494)
                     /locus_tag="IXT53_001254"
     CDS             complement(8607..9494)
                     /locus_tag="IXT53_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ4905095.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9510..10793)
                     /locus_tag="IXT53_001255"
     CDS             complement(9510..10793)
                     /locus_tag="IXT53_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ4905096.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(11476..12159)
                     /locus_tag="IXT53_001256"
     CDS             complement(11476..12159)
                     /locus_tag="IXT53_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAQ4905097.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12177..12794)
                     /locus_tag="IXT53_001257"
     CDS             complement(12177..12794)
                     /locus_tag="IXT53_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HAQ4905098.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(12804..13628)
                     /locus_tag="IXT53_001258"
     CDS             complement(12804..13628)
                     /locus_tag="IXT53_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HAQ4905099.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(13621..14181)
                     /locus_tag="IXT53_001259"
     CDS             complement(13621..14181)
                     /locus_tag="IXT53_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905100.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14355..15311
                     /locus_tag="IXT53_001260"
     CDS             14355..15311
                     /locus_tag="IXT53_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ4905101.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(15616..16572)
                     /locus_tag="IXT53_001261"
     CDS             complement(15616..16572)
                     /locus_tag="IXT53_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HAQ4905102.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            16676..17278
                     /locus_tag="IXT53_001262"
     CDS             16676..17278
                     /locus_tag="IXT53_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ4905103.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(17366..18865)
                     /locus_tag="IXT53_001263"
     CDS             complement(17366..18865)
                     /locus_tag="IXT53_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HAQ4905104.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(18881..19405)
                     /locus_tag="IXT53_001264"
     CDS             complement(18881..19405)
                     /locus_tag="IXT53_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905105.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            19703..20452
                     /locus_tag="IXT53_001265"
     CDS             19703..20452
                     /locus_tag="IXT53_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAQ4905106.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            20443..21228
                     /locus_tag="IXT53_001266"
     CDS             20443..21228
                     /locus_tag="IXT53_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ4905107.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            21197..21913
                     /locus_tag="IXT53_001267"
     CDS             21197..21913
                     /locus_tag="IXT53_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ4905108.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(22015..23034)
                     /locus_tag="IXT53_001268"
     CDS             complement(22015..23034)
                     /locus_tag="IXT53_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HAQ4905109.1"
                     /translation="MRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIVSAKDH
                     DAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVPSYSPN
                     AIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRIGLTEA
                     VLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQMINEG
                     FLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSFKPWET
                     IPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNEVSLPK
                     ARQLR"
     gene            complement(23053..24111)
                     /locus_tag="IXT53_001269"
     CDS             complement(23053..24111)
                     /locus_tag="IXT53_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014372913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HAQ4905110.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            complement(24332..25480)
                     /gene="nagA"
                     /locus_tag="IXT53_001270"
     CDS             complement(24332..25480)
                     /gene="nagA"
                     /locus_tag="IXT53_001270"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HAQ4905111.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(25556..26392)
                     /locus_tag="IXT53_001271"
     CDS             complement(25556..26392)
                     /locus_tag="IXT53_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ4905112.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(26576..27793)
                     /locus_tag="IXT53_001272"
     CDS             complement(26576..27793)
                     /locus_tag="IXT53_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ4905113.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            28176..28259
                     /locus_tag="IXT53_001273"
     tRNA            28176..28259
                     /locus_tag="IXT53_001273"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28210..28212,aa:Leu,seq:gag)
     gene            complement(28499..29497)
                     /locus_tag="IXT53_001274"
     CDS             complement(28499..29497)
                     /locus_tag="IXT53_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905114.1"
                     /translation="MGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEINRKIYWVS
                     IPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELIFSLTTPGN
                     ALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFFLDMNILFLLLFFLIFLLTYRKC
                     QNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLS
                     LFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVW
                     ASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
BASE COUNT         8844 a         6303 c         5063 g         9362 t
ORIGIN      
        1 tatttttgct attctatttt aattgaattt tgcaactcac tcttctttat tccaaaccta
       61 ctcgtttgaa tatcacgtca acgtttttca aatgatgatg ataatcaaag gcatcgtcta
      121 tttcttcttt agataatacc gatgtgatgg ttggatcttc ttctaacagc gggcggaact
      181 gcacttggtt gtcccaagcg tacgcagttt ttggctgaac aagatcataa gcttcttctc
      241 ttgtcattcc cttatcgatc aatttcagca taacgcgctg gctataaatc aaaccaaatg
      301 ttgcagacat attccgcttc atgttttcag gaaagaccgt tagattttta acgatattgc
      361 caaaacgatt taacatgtaa ttcaataaaa tcgtggtatc aggcaagatg attcgttctg
      421 ctgaagaatg agaaatatct ctttcatgcc ataatgtgac attttcatag gcagtgacca
      481 tgtgtccgcg caccactctg gctagaccag ccatattttc tgaaccgatc ggattccttt
      541 tgtgcggcat agcagaagaa cctttttgac ctttcgcaaa aaattcttct acttcacgtg
      601 tttcggattt ttgcaatccg cgtatctctg ttgcaaaacg ttcaatactt gtagcaataa
      661 gcgccatcgc tgagaaatat tcagcatgta gatcccgagg gagaacttga gtagagattt
      721 cttgtggacg gatccccaat tgtttacata catactcttc aataaatggc gggatatttg
      781 cgaacgttcc cactgctcct gagatttttc ctgcttctac tccttttgcc gcatgttcaa
      841 atcgttcgat gttccgtttc atttctgaat accatgtggc aagtttcaat ccaaaagtag
      901 ttggttcagc atggacaccg tgtgtccgac ccatcatcac tgtatatttg tgttcttttg
      961 ctttttcgcc aatgatttcc gtaaaacgtt tcaagtcttc ccgtaaaata tcatttgctt
     1021 gcttgatgag atagccataa gcagtatcta cgacatccgt acttgttaga ccataatgga
     1081 cccattttct ttcttctccc aatgtttcag aaaccgctcg tgtaaacgca acgacatcat
     1141 ggcgtgttaa tttttcgatt tccaagatcc gatcgatatc aaatgatgca tgctcacgaa
     1201 tttttttcac atcttcttgc gggatttcac caagttctgc ccaagcttca tctgctaaaa
     1261 tctcgacttc gagccacgca cggtatttgt tctcttccgt ccagatattc cccatttcag
     1321 ggcgtgtata acgttctaac atgttttttc tcctctaatc ccaaatattc gtttgtttga
     1381 tttcttccag cgtttgctgg atgtcttctg tcaagatagt gatatgtccc atcttgcgac
     1441 ctttttttgc ttgttttttt ccatagaaat ggaattgcca gtctggctta ttctcaatca
     1501 agcgatagct ttccagcagt tgttctccca aaacattgac cattactgca ttgcttatca
     1561 aacgtacttt ttctcctaat ggccaaccac aaatacctcg aatatgggca tcgaactgac
     1621 tcatagaaca agcttcaata gaataatgtc cagaattgtg aggacgagga gctaattcat
     1681 tcacatatag cccgcctgtt tttgttaaaa acatttctac ccctaaaaca cctgaaaggc
     1741 tcactgcttc tgcaatcaca cgggcaatac gctcagcctc ctcgatcaca tcctgatcaa
     1801 tcgcggctgg agcaatcgtt tcatgaagaa tattgttgtg gtggatgttt tccacgaccg
     1861 gaaaagtcgt gaaatctcct tgaccatttc ctgccaccat gatcgaaatc tctttttcaa
     1921 acgggatcca tgcttctaaa acacaggttc cttctcttaa aaggtccatg gcaggagcta
     1981 gatccgcccg acttttcaaa acatactgcc ccttgccatc atagccgcct cgagtggttt
     2041 tcaaaacaca aggatagccg atcccgtcaa tcgcatcttg gatatccgtc ggactgacga
     2101 tcgttgcata aggagcaatc acgatgttat tcgtttccaa aaaagatttt tctaacaggc
     2161 ggtcttgcgt aatcgcaagc aagtctgtcc cttgaggaat aaatgacatg ggtaaaatcg
     2221 catttaacgc ctctacactg acgttttcga attcataggt aatgacatct gttcttctgg
     2281 caagctcttt cagcgcaaag gtgtcatcat agacactttc aatgtgccaa tctgcaactt
     2341 gtgcggctgg acaatcgctc acaggatcca gcacacctac tttaaagccc atttcttttg
     2401 cactaatcgc catcatacga cctaattgac cgccaccgac gatcccgatc gttgaaccag
     2461 gcaacaaagg tttaaccaag ttgatcacta ctttctataa cagttttagc caacattgaa
     2521 cgtctttcag ccaatttagc ctccaattct aaatcataca tggaaagcat ctgtatcgca
     2581 agtagtccag cattaattgc tcccgctttt ccgattgctg tagtagctac aggtactcct
     2641 ccaggcattt ggacaatcga aagaagcgaa tccaaaccat ttaatgttcg tgattggacc
     2701 ggtacaccga taaccggcaa agttgttttt gatgcgacca tacccggcag atgcgcggct
     2761 ccccctgctc ctgcaataat gattttgatt cctttttttc gagcgttttg tgcatattca
     2821 aacatcaaat ctggggttcg atgagcagaa acgacctttt tttcataaga cacaccgaat
     2881 tcatccaata tttgacatgc ttctttcatt gtttcccaat ctgaagtact ccccataatc
     2941 acagagattt ttgctgtcat tgtttttcct cccagttttt ctacattcat attttaacaa
     3001 tcactttttt tgatgtcaaa gaaatttcga atgttaacaa agaaaaacat tttattgttc
     3061 gtgtttatta aagataaaaa aaaggaatgt gacaaaagtt aggtcacatt cctttttatg
     3121 gataaacggt gttctcctgc caactcctcg gtcacaagga acaatattca acaaacatga
     3181 gaagcttttt tttgaaattg ttccttgtga ctcggagatg aacggcaggc tcacgacctc
     3241 ttccttcaac ggtgttcaat cagctgaccc tcggtattag ctcaaacaaa cggcaaaata
     3301 cgaggaacta tttttccgtt tgtttctcta acaatcaggt caaaacgctg atttcacaac
     3361 ctttcttttg ctctattact tcttgaaggc ttcttccaat atttcttcat cttttttcaa
     3421 tccattaaag atcaaattca atatgatagc cgtaatgcta ctcattacga tgccatttcc
     3481 agtgaacatt tgcaatgttt gcggcatttg actgaataat gtcggcgtaa cattgaaacc
     3541 cagcccaaat ccaacagaaa cagcaacaat caatagattt ttatcattgc tgaaatcaac
     3601 ttccaacagc atacggattc cttggactgc caccatcccg aacatgacta acattcctcc
     3661 accaagtacc ggctcaggga tgatttgtgc taacgcaccg attttaggga ataatcctaa
     3721 aacaatcaag aagaatgcag agaaatagat gggttttctt gttttgattc ccgaaagttg
     3781 aaccaatccg acattttgag aaaagccagt ataagggaat gtattgaaaa taccgcctaa
     3841 gataacggca agtccttctg cacgatatcc ctttttcaat tcttcttctc caacattttt
     3901 cccagtaatg tcaccaagtg caaaatatac acctgttgat tctaccatgc tgaccatgga
     3961 aatgatgatc attagtaaaa tcgaccatac gtcgaaagtt ggtttaccaa aataaaaggg
     4021 ttgtggaaca tggaacaacg gcgcatctcc aatcgcgctc agatcaatca ttcccatgaa
     4081 agcagctaag atgcttcctc cgactaaacc aatcaatacc gcgattgaac gaatgaaccc
     4141 ttttgccagc atttgcacgc ctacaattag gagaatcgtc acaaatgcaa gtagcagatt
     4201 tgttttgtca ccaaaatcag ctgctgttgc attacctcca cccattttct cgattgctac
     4261 aggaatcaaa gtcaaaccaa taacggtgat aaccgttcct gttactatag gagggaatag
     4321 ttttttaatt ttagaaaaga caccagaaac taacacaaca aagatcccag aagcgataat
     4381 agatccgtag atagctccta ctccttcatt ggttccgatc aagatcagtg gagctactgc
     4441 ttggatcgca catcctaaaa cgacaggcaa gccaattccg aaaaatcggt taactgtcaa
     4501 ttgcaataat gtcgctaatc cacacataaa gatatcaatt gaaatcaaat aagtcatctg
     4561 ttcttgatta aagcctagtc ctgtaccgat caaaagaggc accgctactg ctcctgcata
     4621 catcgctagc agatgctgca gccctaaaac cgcagctttt ccattttgtg tttctttttc
     4681 cacgattatg cgtcctcctc aagaaactca acttgtccat tcgtcaagga agcaatacgc
     4741 gcaagagaaa cgactctcaa tcccatttct tcaagtaact gacgtccatc ttggaaagac
     4801 ttttcgatga cgatcccgat tccttctact tgcgctccag cttgctggca taattcgatc
     4861 aatccttttg ccgcttgtcc gtttgccaag aaatcgtcga taatcaatac tttgtcttct
     4921 gaagataaaa atttacgaga aatagaaatc gtacttgtca cttgtttcgt aaaagaataa
     4981 acagaagctg tcaataattc ttcatccatc gtcaaacttt ttgcttttct agcgaagatc
     5041 attggaacat ctaattctct tgcagcaaat aaagctggtg cgatcccaga agcttcaatg
     5101 gtcacaactt tcgtgattcc ttgttcttta aatacttctg caaaccgttt tcccatttgt
     5161 tccatcaata cagggtctac ttggtgagtg acgaagctgt cgacttttaa tacgccctcg
     5221 cctaatatac gtccgtcttt ttgaatacgt tcaactaatt ctttcacttt atttgctcct
     5281 ttatcattta ttaataccga aataaccgct tttgtgcaaa aacctactat gtttctgtta
     5341 aagaaaaaca ccctccactt tatcataaga aaagaagggt gaaatagcca atcttttact
     5401 tatagtccag catttacggt actggctaga aactgtcatc catattatga cgatatataa
     5461 gtaggtatgt aaaactaata atagtgtaaa ttcattttct tgcaactgtt ttgcacgtga
     5521 aggcagtttg ccatatttga ataaaaaatg ttgtttcaaa tattgaaaac agtctaacac
     5581 tactttctga aactgacaac gtggtttact tatttttttg taaaatctaa tgatttttaa
     5641 tgcattattc ctttttgttc gggtttaaat agttattttt attttctcct atctaaaaaa
     5701 gcagcccaca taaaaagaag gcatgacaaa aagcatgtca tagccccttt ttccgaataa
     5761 atagtgttca aaagacagcc tatcggcaat aaggcaagat tttataaact acgggaagta
     5821 atttccgtaa atctattctt attcttttaa gctgacaagc tcgtcaatca ctactgcttt
     5881 tcctgaagca atagaatgat ttgctgcgat tcctgtcaaa atcgacaaaa taccatctac
     5941 gtgagttgct gcccgattcc acggatcttc ttctttttct tcaccaaata gatcatctaa
     6001 taaacgcgga tctcctccgc catgtccgcc cgcttcttgt tcgacgatta cctcatatgg
     6061 ctcaccgaac atcggataga catggatgga ctgaagaacg gctgctcctt catccttttt
     6121 atctccacct gcattgatgt aagacttttc gatcactttc atttcgatcc gtcctttgct
     6181 gccattgaaa gaaacattga atccttccca cggcatatac gcgtttagcg aatacgtcaa
     6241 aatcgcacca gtttcatatt tgaccgttac gcctagtgta tcttcaatag agatatcatc
     6301 ggaaaataca ctgcggtcac gaatatatcc actgtctttt tctgcttcta aatacatttc
     6361 tcgcaattcc tcattggctg acaggtctat agcaaatgga tcagctttag ctagttcgct
     6421 tccatagcat cgactgtaaa atatttctac tccgcgtttc tccgcattat gcatcccata
     6481 aaagtttaat ccaccgaatg caaatacctt ttggggacgg ctgttcaacc aaaaattcac
     6541 tagatcaaaa tgatgggtag atttatgaac aagaagcccg ccgctattgt gcttatttcg
     6601 atgccatctt ctgaaataat ctgccccgtg ttgcgtattt aacagccatt cgaagtggat
     6661 agaaaacaca tcaccaatca catcactttc tatcagttcc tttatcttcg tgtgatacgg
     6721 tgcataacga taattaaaag tgactcgcag gcttcttccc gtctgttttt ggaccgttat
     6781 tatttcttga gctttttcct catcgatagt cagaggtttt tcgcaaataa catcatatcc
     6841 ttttttcatt gcacgaatga tataatcatg atgcgttcga tctactgacg taataatgac
     6901 ttcagccggc cgtacgtcat ccagtagttg ctcaaattgt gatggcatac atgtctttac
     6961 ttttttctgt ccatacttgt tcacaagtat ccgatttgca taatccatcc ttgtttgaga
     7021 tacatcacaa aaaccgacta gttcacaagt ttgatgatac gtagaggcaa ttgtctcata
     7081 aaaaaaacgc gctctcccac caagtccgac ttgtacatat tttttcttca tttaaagtca
     7141 ttctcctttt cttttaagca aaatactgtg acaaatggca gccctggata cagtaatgca
     7201 gtgagtaaaa aatcatgctg tatacaccag aacagtccat acaaaaggca taaataccac
     7261 agaaacccct tgacgttttt tgacaacagc cagatattcc ttttgatcaa ataaacaaga
     7321 ctatctgctt tatcttcttt gcagatatca attagcgtac taaaaaaact atgggataca
     7381 aaaagcaaca gacacaatag aaaaggaatc agataccaag ctccaatctt tcccactaat
     7441 aaataccaat caactgcaaa aataatgaca aacagctgaa acaacgtaat gatgaaacac
     7501 atccgtttga tttttccttt cctccaacat accttttttt ctgccaagta atggacagat
     7561 agataataag aaggatacat catcaaaaaa taaatcagat agacaacacg agcagacagc
     7621 actaatccta aagtgaacaa acatcctaga actaaagcga aaagagcatt actgataaga
     7681 ctggttaaaa aaatccgaca gacatagtag ttgagcttgt aaagaaacat ctcttctatt
     7741 ggctttttca tattcagccc ttcagaccgc ttgtactgat cccctggaca agatatttat
     7801 tgaagaataa gaaaatcaga aaaaccggta gcagcgacaa cgtagacatc gcaaacatac
     7861 tcgcgtaagt cgtactagat ccagcgtctg caaataattt gatggcaatc gataccgtgt
     7921 aagatctcgg cttgtttaaa taaatcaatg gtcccatgaa gtcgtcccat ttccaataaa
     7981 actggatgat gatcgtcgtg atgatcgaag gtttcagcaa aggaaaaata attttggtaa
     8041 agacactata cttgctgcat ccgtcaataa tcgctgattc atctaactct ttggggatgc
     8101 tgaccatgaa ttgcatgatc tggtaaatga aaaatgcttg tccaaaaaaa tgtggtaaaa
     8161 tcagcggcac atacgtccca accaaattca gccgattgta aataataaac tgcggaatca
     8221 aaatcacttg tgcaggaatc atcatcgtta ctaacatgac gacgaaccag aatctttttc
     8281 ctttgaaatc gattctcgat aatgcgtaag caatacaagt cgagctcaga actgtcccta
     8341 tcgtcgaaac tgcagagatg acaaacgaat tcctaaagaa agtaaaaaat gaaattccag
     8401 caaaccccgc ccatcctttt gcgtaatttt cccattgaaa agtctctgga atcaaattca
     8461 gcgaaccact cataatctct tgatccgttt taaaagaacc tgcgatcatc cataagaccg
     8521 gataaatcat gactagacct aatagcaaaa taaaaccgta ttgaaaccat ttcgttactg
     8581 tattattcat ttcttcctcc tctcattcac acaacgaccc atttttttga tgatttgaaa
     8641 atcagaaagg tgacagctcc cataatcaac agcatgatcc acgaaatagc actagcatac
     8701 cccatagaat taaaagaaaa agcttggtta tacacataaa gagcataaaa gttcgtggca
     8761 tcattcggac caccttgggt aatgataaat gcctgcgtaa aagtcataaa cgccgcgatt
     8821 gtctgcataa ccaagttata caaaatgatc ggcgaaagaa gaggtaacgt gatcttgtaa
     8881 aacgtctgcc atttgttcgc tccatcgatc tctgccgctt cataatacga gcctgggatc
     8941 tctttgatac ctgcagcaaa gatgatcatt gccgaaccga attgccaagc agacatcaaa
     9001 acaagaggga tcatgaccag tttttgatcg ctaaaccagg aaagcgcagg tgcgcctaat
     9061 cgttccagga aaatattcac taacccgttt ttagaaaaca actcccgcca aactagagcg
     9121 atagaaatac tgccaccgat caaactaggc acataataca aagaacgaaa gaaagtcgtt
     9181 cctttgatat tcctcgttaa aagataagca atcaatagac caaagatcag tttcacaggt
     9241 acagatgcaa atacatagag aaaagtgact cggatggagt tcaaaaaacg ggcatcactc
     9301 agcatcttta tataattatc caagccgatc catgagactg gatttcccaa acgataatcg
     9361 gtgaacgaat aataaagtga caagatcatc ggtatcactg ttatcaatag aaatcctagg
     9421 atgaatggag aagcaaatac gtaaccgacc acattgtctt gatccaagaa ccgttttagt
     9481 ttttcttttt tcatgccttc ctcctgctgc tatcctagaa tgtttgacgc aaactcataa
     9541 agactctctg ctgcttcttc tgctgttact tcttcataaa tgactttttc aactaaaaga
     9601 ttgtattggt cttgaatttc cggattcaaa gcactatcaa agacattcac ttcgccgttg
     9661 tccatatcgg aaatcatttc taaataatca aatgtcgctt gcaaagttgc atcatctgca
     9721 cgagcgagct gttctcgaat attcgacatg attggtacac cacgttctcc ttttaaaatt
     9781 tcattggctt cttcactgtt ttgaaagaaa tcaataaact tagcagcttc ttctttatgg
     9841 gctgagcttt ttgccatact cagcatttgc gaagaacgta aagggatacc agattctcca
     9901 tctgcttttc tgcgaggtgg attaatcaac tgaatctcca tatcttcagg agcccctgac
     9961 aatatttctg tcaactggtt acttgataga aacaccattg ccgcttctct atccctaacc
    10021 gcctgaaaag acagatccgt cgtacttgca gctccaccac ctggttctgg atgggcccct
    10081 ttgtctacca gatttttacg cagctggaaa aaatctgtta gatagtctgg attttcaaat
    10141 cccaagctgt tagatagatc ttcccgatta taaaaattca atcctttctc aacttgtggt
    10201 atttgaataa tacagccagc tataaaatct tcgaatcttg aaatagcata gattcctaat
    10261 tcttcagtga tttgatagct gatttgttca aattcatccc atgtccaatt tgaagctggt
    10321 tcagggatat ttaattgttc aaatatattt ttattgtaag caatcgcaaa cgaattcacc
    10381 ccgttagaca gtccaactaa ttgcccgtcg taagtcgtcg ttgctaaata ggattcatct
    10441 gcattagaaa tatcaattat cccttgctcc acatattcat ctaatggttc aatctgattg
    10501 atgtattgag gaaattgatt gttcagttgg aaaatgtccc agatatcatc tgatgctgct
    10561 agtgtaccta attttgtgta atatccttca taatcataaa attcataatc gatcgtcaca
    10621 tggggatttt tttcttcata aagctcgatt acttttactg ttgcatcgtg tcggacttgc
    10681 gaaccccacc aagccatgtt cagcgtgatt tttttgtcat cttcttgccc acaagccgtc
    10741 aagaagaata acataacact taaagctaag atgattctca ttcttttctt cattctctgc
    10801 acctctactt ccattgttat ttttcaatat actgtatttt tatacagaac atatttatta
    10861 aacgatcttt ttcaagggga ataaaaacca agacttgcct tatcgttctg cctaggatga
    10921 acaactcaat actttcaaga aaaattagat agtgctgatt cttctttcat cgctaaaaac
    10981 acttcttgct tcaatgatgg gaggataatt acgcaaagaa aaaaggatat cagacaactg
    11041 cccaatatcc ttgacatagc taaataagca tgcttcttcg tgtgcgctct tccctaaaaa
    11101 acagaaaaat aactagtaat tctgtatgaa attaggtaca atatacacgt cgatgcacaa
    11161 gcattgacta tggcaacttg attggtcagt tcccgctgac ttttcaagcc ccgacaattt
    11221 agctgctact aaattgtcgg gtgccttttt tagtttttat tcgtttttca ttctatagaa
    11281 agcgcttcaa taaatcaaga catttttttg ttagaatgct aataataaca aaaggtttta
    11341 actcttctta tgtacattac aggtaaaaaa acaaaaaaat ttctcttttt tttgaattct
    11401 gaacaaatag tactggagta caaaagacga ggaagagcca ctatctgctc ttcctcgtct
    11461 tttgattctc acctgttatt catgattctt tttctcagtt tcccgatatt tttccatttt
    11521 acgactaaaa agctctccgc tacttggtgg cgtatatgtg atggcatttg ctcctgcttg
    11581 aatggtctca gcaatacttt cttccgttgg accacctgtc gcaatgatag gaagttcagg
    11641 atatttttta cgaaaatggc gtactgcttc tgcagtttct ttacctgcac tgatattgat
    11701 aatcgaaata ccagctgcta acttttcatc tatttctgta tatttcgacg ttaccgtact
    11761 gataactgga atatcaacta ctcgacaaac ttcttttact gtctctaaag gcgtaggacc
    11821 gttcaaaacg acccctaacg aaccttgcgc ttctgcgaac atgctcatgt aggaagaacg
    11881 agcaccttgt gtcaaaccac cacctacacc ggagaaaact ggaatgtcag ctgcctcaat
    11941 aatgctttta gtaatcgctg gatgaggtgt aaaaggatac acagcaatta ctgcattcgc
    12001 atttgtatta cgtatgatcg caatatccgt agtaaagatg atcgaacgga tcaattttcc
    12061 aaatatcttg atcccgcttg cttctaaaat gatttccggt actcttacga tatcttttct
    12121 caattccgtc atgatttctg gcgtaaattg ttctgccatc agccacttct cctttgctat
    12181 ctccggtcat actgattcaa ttgatacgtc tcgatgacct ggatgatttt ttgcgcataa
    12241 ccggggtctg tcgcataacc tgcatcttgc aatgcttgtg cggcttcctg atagttggtg
    12301 gccgtaatca ctttttcata cagcgcagga ttccaatcta ctccttggac gaacagttgt
    12361 gtatggtcat ccatggactg ttcccatgaa tcatacactt tgaagtctcc ttggatagtt
    12421 atccattctt cgttaacaaa ttcctttgtt tccaggctta ctttcttctg atccccataa
    12481 gccttgatcc cgaaaagatt attgtatttg ctagccaagg tactttttcc ccaatttgat
    12541 tctaatatag cttgtcctaa aatgatacta ggaagcactc cataaccatc ttgtagttct
    12601 tgggcatgcg gaacaagccg ttcaataaat tgctgatggg attgcgcttc attttcttgc
    12661 tgattatcag taaaaggaga agacagattc ttcaaggaaa aaacaaaggc taagagtatc
    12721 actgctacac cggcaaccac tgctggccaa cggatattct tgcgcttttg ctttttccac
    12781 ttttttttcg ccatgatcaa ttcctacttt tcatttaatt cattcagata ttcttctagc
    12841 gtcaattggt gtttggtgat atactcagcg ttttctttac cgacataacg taagtgccaa
    12901 ggttcatatg tgatcttagt tatatcttcg tgtccttttg gataacgaac gataaatcca
    12961 tactgcgcag cattatctgc gatccattta gctccaggct gatctccgta acttgcatcc
    13021 aatacagtag aaggataact gttgtaccag ttttgatcga cgacatcgac tgccagacct
    13081 gtgtggtgtt cgctgtatcc tggctccgta atcgtttctt tagtaatctt tgtcgcctcc
    13141 tcttctgata caccttgacg actcatcaca tcttggacat tttgttcgaa aacttgttgt
    13201 tgggatgata cagaccggta agctgagacc atcactaatg gaaatccagc tgcttcagca
    13261 gccttggcaa atgcttcata ttctggagca atgcgtttgt cgatcaagta tccgttactt
    13321 aatgatgcca attgagtaga ctcatcaatt tctttttcta atttgtgatc tggaccaacc
    13381 aaaactagct tccaatcgtc tgttttaaca tctggcaatt gtttggacgt acgatctttt
    13441 gtttctttgt cctttacctt gctgctggtt gtttgactgt tcttttcttt actagcagtt
    13501 gttttggtgc ttgttcgttc gactgcttga ttgccggctt gaacatgcga atcatcatcg
    13561 ctaaaaataa catagcccat tgctgcgatc ataatcacaa ctgctccaaa tcctaaaaat
    13621 ttattcactt gaaaaatcct ttcgttgttc ttcacgttcc ccattcgcct catgaacgta
    13681 agtttcttcc agattttcat tcacccaatc gagaagttcg attttttctc cttcgatctg
    13741 tttttgaaca tctgctggca gacgaaaatt caaaatatca tcatagcccc attcatcata
    13801 ttgaatactt actttcaagt caaggtatct accagcctgc tgattatttt tcaaagggat
    13861 cagctcgact tttgctgctt tgctgctaag ccatttttgc gcgatgcgtg ttttgatttg
    13921 cttgtactct gcaacggcaa cgtttcgttt ttcgggaaaa ataatcgttt gacgcaaaat
    13981 cttttgcgtt agcatccatt cataatcagt gaaaagattt tttactttct gcatcgcttc
    14041 agatggcaga tttttctcgc catctttcca tttttgccat tgttctggac tgatcagcaa
    14101 gtattcatct aaaaaatttt tttcagtttc aaactgttct aataatacac tcataataag
    14161 atcaatatat acttgatgca cgtttatccc ctccttttaa ctattctact taagttttga
    14221 tcaaaaagca cctcttaaat cgctgctttc atttttttta atgtttcaag tcctctcctc
    14281 tttattcttc gtgatttttg ttataataat agacagacaa gaaactcaat ccaactatga
    14341 aggagcgttt tcagatgaaa aaatatcaat ggggaatcgt tggtcttggt accatcgcac
    14401 atgagtttgc cgaaagcttc aatcaagaga cgagcgaact aacagcagtt gcttccagaa
    14461 cttcagaaaa agcggaaaac ttcgctcacc gttacaatat tccaaaagct tacggaagct
    14521 atcaagaaat gttagacgat gctgaaatcg acatcgtgta cattgctgta ccaaatagac
    14581 agcatatcga ccatatcttg gcagcattgg aagcaggcaa acacgttcta tgtgaaaaag
    14641 ccatcacgat gaataaaaaa gaactggctg acgctatgcg cttagcagaa gaaaagaatt
    14701 tgatcttagc agaagcgatg acaatcttca acatgccact ttatcagcag ttacgctcaa
    14761 taatggatac aggaaaactg ggggctttaa aaatgatcca agcacctttc ggcagttaca
    14821 aagaaccaga ccctaaaaac cggttcttca atccagaatt agctggagga gctctgttgg
    14881 atatcggtac gtatgctgta tctttcgctc gtttcttttt gagctctcaa cctgaagtag
    14941 ttgcctctac aatggttcct tttgaaacag gcgtggacga acaatccgtc actatcttgc
    15001 gtaacaaaga aaatgaactt gctgctgtca gcttgacttt tcaggcaaaa atgccaaaag
    15061 tcggtatcgt tgcctttgag aacggttatg tgacgattgc tgattacccg cgtgctgatc
    15121 gtgcggagat cttatttaca gatggtacaa aggaattcat cgaaagcggg aatacatctg
    15181 aagcaatgaa ttacgaaata aagaatatgg tacacatgat tgaaggtgac ttgcccaatc
    15241 gctctctctt cttgacaaaa gatgtcatcg aaatcttgga tcaaatgcaa gttctatgga
    15301 aaaatatgta attttaaaag gttgtgaaaa agctgttctg catcaagtaa taagaacagc
    15361 caaacaaaaa tagctcctcc tatttttcgt ttggctggac ttattaccgc agaggaaagc
    15421 ttttgaacac cgtttaagaa agaggttgtg agcctaccgt tcagctccga gtcacaagga
    15481 gcaatttcaa aaaacagctt ctcatgtttg ttgaatattg tgacttgtga ccgaggagtt
    15541 gacaggagaa caccgtttaa agaagaggtt gtgaaatcag ctttttgacc tgaatattag
    15601 ctgaattcac aacctctatt tttggatttt ttgttgggag aattctgcct tgatttcaat
    15661 aaaactttta aaagactgca aaaactggaa caattctgct cgatcctcga attcagggcg
    15721 actagtcggc agaggcattt gccgatacaa tacataaact tcttcaatac gtgttaaaat
    15781 ttgtctgcca tcatttgact ctgaaaatgt ttctgcagta taaatcaata aaccatagat
    15841 atgttttcca taataagctg gttgctggat tctctctaga ttttccagca tgtctttaat
    15901 cacattggcc tgtgctcttc tcatagagaa gtaagtttcg tagtatagcg gctgtctgag
    15961 ccagtagttt tcttgatgtt ctctggcatc ttcttggctt tcgcgaatga atgtcagtaa
    16021 atgttcgcat ttgatctgca catcttcttt attttctgat aaaagaaatt ctgccatttg
    16081 tctcaaaata tttcgaaatt ctttttcgat ctgtatttgg ttttccttca gctgcttcgt
    16141 acgatctggc atgtaaacat ttgcaagcaa agcaaatact aatccaatcg ccattaatcc
    16201 cgcttcattg ccgatcagct gccaagacat gttttcctcc actaaataat gagtgaccaa
    16261 aaccgaattt actactatcc cttcggttaa tttgaagcga gaggcaagcg ggatgaacag
    16321 gaataaaaaa agactgaagc caaaaatagt atacccaaca gcaaaaaaac tgaacagtgc
    16381 tgttgccgta gctaatataa aggctgcgat tcgattcatg cccgtacgca atgtagagcg
    16441 cttggtattt cctacactta ataatgcaat gatccctgca gctggccaat acaacagcga
    16501 taagctaccc gctgtgacca tcgaaaagaa gacactgatc actgttttga ctgttcgcat
    16561 tcctatacgc atttacttcc ccctttttta ttttaattat acttgaataa tgaaaaacta
    16621 gctatcagac aaaagtttag gtatgatagt aaaaagacca aaaaggagaa tctctatgca
    16681 atttggccgt tttcgactag gtatgcgtac gatcaaaagt gccttagctg tttttttatg
    16741 tatcttgttt tttcatttga ctcaacgagg aatcccgatg attgctgcat tatctgctgt
    16801 tttttctttg cgtcaggact tgactacaac cgtttctttt ggaaagtcta ggatcatcgg
    16861 caatacatta ggtggtacat tagctatcat ttattttttt gtcaaaagct attttcacaa
    16921 tgattttttg gtagaattga tcttgcttcc tattctagtt attattgtga tcgtcgtgtc
    16981 agatggtatt aacaataatt ccggtatcat ttccgcaata gctacgttgt tgctgattgc
    17041 gttgagcatc cctcaaggcg aatccgcttt gtatgctatc caacgtgtac tagatacttt
    17101 tatagggaca ttcattgcaa tcggtatcaa tttctttctt cgtcctcctg aaacagaaaa
    17161 gaaggaagaa ctatcagaag atttggtcga actccaaaag aaagaacgat tgctaagaac
    17221 aaatttacaa gagatagaag ctaagatcca aaaacaaaag aaaaatagcg aggactaatt
    17281 ttcttttgca ataaaataaa aaaatggaga gaagaacttt aaaaagtctt cctccatttt
    17341 tgattgaacg gtgaagtgta ctactttatt cttccttcat cgagttttgt tctgcctctt
    17401 ttggcgcttc atacatattt acttggctag tagtcccacg tgtcgacaaa ttctctacga
    17461 tttccttcaa gtcaagtcct gttgtcgctt ttagtgtttc ttgagtagaa gaaagcaaat
    17521 ttgtggcata atttgtcact cggttcgctc cactggtttc tcctgaactg cttcctgtat
    17581 cgacaacaga gattttttcg atatttccta gcggctgtgc tgcttcgcgc atcaattgcg
    17641 gtagcatatc gataaccata ctcaagacag ctgcttcgcc atattccttg aaggcattgg
    17701 cgatcttttg ttttgcttcg gcttctgctt ttccttttgc tagtgctgct tcggcttctg
    17761 cttgtccagc taaacgtgtc ttattggctt ccgcttctgc cagtgcttcc acacggaatc
    17821 gttctgcttc ggcttcagtc acttctttga ctttttgagc ttgtgcttct tgttctttag
    17881 cgtaccgatc ggcatctgct tttttcttca cttccgaatc atattgcttt tcacgacgtg
    17941 tgatttcttt ttcttccagt tcgatttgtt tttgacgttc tacgatttta atctgcattt
    18001 cctgttcgat tacttgttgt tgtgctcggg cactttctaa attgtaggct tggtctgctt
    18061 ttgctttagc aacatcttgt tcttgtttgt aggttgctaa tttcagttct ttttctttta
    18121 atgattctgc gatctctgtt tgtcgctgta attcagctgc ttgtgattct ttttctgctt
    18181 gagctttttt gatacgcgtt tcttttagag cttccgcttc tgctatatct gcatctcttt
    18241 tgacttgggc gattcttggt ttccctaaag agtcaagata accgtttttg tctctgacct
    18301 ctttgatagt gaaagaaacg atgatcaacc ccatttttgc aaggtctaca gaagcgactt
    18361 cttgtacgct ttgactgaac ttatcgcgat tttgatagat ttcttctaca gtcatagaac
    18421 caagaatcga gcgcaagtgt ccttctaata cttctcttgc ttcattttcc agttcttctc
    18481 ttgtcttgcc taaaaactgt tctgctgctg tagcaatctc ttctacagat gaaccgattt
    18541 tgatgatcga agtaccatca cacatcaccg gtacgccttg ttccgtatac acttcagggg
    18601 tggagacgtc caatttactc gataatagac tgatccgatt cgaacgttga aaaactggca
    18661 agacgaatgc gccacctcca cgcacaattt tgattttgtt attgctttca tcaatgtgca
    18721 catttttgct tcctaaataa cttccactga tgatcaacgc ttcatccggt tttgctgtct
    18781 ggtacttcgc tacaaagacg atcagcaaca tcagtaaaat caaagctgcg attgcgatcg
    18841 cgataaaaac aactggtatt tccatttaac acacgctcct ttaagctttt atcatgcttt
    18901 cataaggcat gacataacag attctttcct tgatctcaat gatcaatact ttcgttcccc
    18961 gctcagctgt ctgttcttgg gggctgtaaa aacaagctgg acgactaatg gaaccggtaa
    19021 cacttttcaa ctgaatttct cccatacctc gtacagggat cggtgtgatc acaacagcta
    19081 cttgtccttc cagagttttt tcagaagacg acatcgttga ttcggcatgt ttcatcggca
    19141 tcaatatata gaaattaagc aaaaatacca acactatcga tatgcctcca ctagcaataa
    19201 ataaaaataa agatgattga tctgttaaac gctcacctag aaaaccaaat aagcttgtga
    19261 aagttaacca agggataata agcatcggat caagcgggcc atcaaaatcg aatacatctc
    19321 caaaaataat caataggact gccaatcctg cacaaatcag caatgtatac caataaatcg
    19381 tctcaagtgc gattccccct atcatataca cgcctccttc catttctacg tctatcatac
    19441 caatgtttga aaattctgta tagcttcttt taccgactca gtggtgtttt ttataggaag
    19501 gttgtgttta tcgttgacaa tttgttaaat ttttgttttc atgttaaaat tttcacaata
    19561 aattctttcg aggtagccta tgtttccttt ttttaagaaa aaaccattaa agctccagaa
    19621 cacgatcatc cttgtcgttt gtttctccac attcgtttct ttgattgttt ctgccatatt
    19681 gatcaggaat tttattcttt cgatgaaccg atctagtgtg tcagataaaa tctctagcat
    19741 tgctaaaatc gttgccaatg ataaactcat ccaaaaaaag ctgcagactg gtgacccgac
    19801 acatgcgatt caaggaataa caaatgaaat catgattgca tcgaatttag attttatcgt
    19861 tgtcatggac cctgaaggca ttcgatattc tcacgcagac ccaagttaca tcggtcttcc
    19921 tttctcaagt catgaagatg tgaaaaaagt gattggagga caagaatttt tctctgaaaa
    19981 agaaggaatg ttagggttcg gcttccgcta tttcgtccct atttattatg atcaagagat
    20041 tattggtgca gtttgtgttg gcttaacaaa caacacgatt gacaatcaag tcaaagatgg
    20101 acaaaaaatg attgtttttt cattgattat tggacttagc gtaggcatca taggcgccgt
    20161 ttttcttgcc caaaaaatga aaaaagtact gcttggactt gaaccacaag aaatagcaga
    20221 acagttaagt gaaaaaacga ttatcgaaaa tgaagtagat gaaggaatct tagccatctc
    20281 ttctgataaa actatcctct tgatcaatac acctgcaaaa aacattttct accaaacaga
    20341 gcagctattg gatttcgaag taggaaaaaa atcagtgata atctatatga cgttttgttt
    20401 aaagagacga ttgagcatca aacgaccgtt cggactcaat ccatgtattt aaatgggata
    20461 gaaactattt ctagcgttac acctcttttt agcaatgacc gttttttagg agcagtcgca
    20521 actttacgaa atcgatcaga agtaatgaaa ttaatggagg agcttggggg gacgaaacaa
    20581 tatataaact ccatgagagc tcaaacccat gaattcatga ataaacttca tgtcatttct
    20641 ggtttgattg atttgaagaa gtatgaagaa gtcacccatt ttatccagca gctaaatcaa
    20701 aattataaaa aagagattgg ccaagtctca agtctgatca aagtacctgc aattgccggt
    20761 tttttattag gcaaaataaa cgaagccaaa gaacaatctg ttgaaatgct gttagaaagc
    20821 gattctttta tccctaaaat ggagatggat gactccatcc attcactttt gcaagtgtta
    20881 gggaacttac ttgataatgc aaaagaagcc gtgctacaaa aacatgaaag aaacggacag
    20941 gtccggattc atttaaacta tgacacagaa gctgaagtat tcatcgtgac cgtctgcgat
    21001 gacggtgtcg ggatatcaga agaaatctca gaaaaaatat ttacgctagg tttttcgacg
    21061 aagggaatca atcgtgggtt tggattgaat ctcatccatt caatcgtcaa acgccacggt
    21121 ggatttttag aattttccaa acaaacaaat ggcggtacaa tcgtctatct agaattacca
    21181 aaaaaggaag atcaaaatga tgacaaatgt cctgataatc gaagatgatc caatggttgc
    21241 agaattgaat cgtacttttg tggaaagcat ccctaatatg aacgtaatcg aaagtttcag
    21301 aaatgtagaa gaaggaaaaa aatatttcag acaaaaaaac gcagatctgc tgctgcttga
    21361 tgtatacatg cctggcgaaa gtggtatcca ttttttatct tggcttagac agcaaggaaa
    21421 tgatatccca gtgattttga tcactgctgc agatgatatc gaaaccataa agaatgtgat
    21481 ccgctacggt gccttagatt atttgatcaa accgttcact ttgcaacgtt ttcagctggc
    21541 gatcgaaaag tatcttgctt tctctcagac aatccaaacg actgaaaaag ccgatcagaa
    21601 attgatcgat caagtattca accacaccac tcccattcac gaaataccat cattcatcga
    21661 tttgcctaaa ggtctgtcaa aactgacttt aaaaaaaata agtgcattta ttctaaaaga
    21721 agatactatt ttttcaacaa aagaactggc tcaaaaaagc tcactgtcac gtatatccgt
    21781 caagaaatat ttgaattttt tagtagaaca agatatattg aacgaaagaa tcaaatattt
    21841 ggaaatcggc cggccgctaa cctgttatga attgacaaag aatgggcgga aaaagctcac
    21901 agattactgt tgagtcatcg aattatccca tacaaaatag tccaagtata cagaagtaac
    21961 aactgtttac ttggactatt tttaaacatt ttattgacga atatccatgt tttcttatct
    22021 taattgacgg gctttaggaa gagatacctc atttttcgtt tttccagttt ctattatttc
    22081 acgaaagttc tcgaaacttg tcgaaatcat atttgataat gcctcatctg tatttgaacc
    22141 tacatgcggg gttacaagaa ctctagggta catttctact aattgctgga tagcagggtc
    22201 tggaatcgtc tcccatggct tgaaggatcg gaaaaaaacc tctgtttcat ttgcaaaaac
    22261 atcggttgca aaaccagcta aatgattgct tttcaaagct cttaaaatag cttcattatc
    22321 ttgaagctct ccacgggctg tattaatcaa gatgctacct tttttcattt gagctaaaaa
    22381 tccttcattg atcatttgat cgttttcacc tgggatatga ggtacatgaa tactgacaat
    22441 atcgcttttg gctaataact cttcaagcga gtggaacgtc aatacttttt tagcagcttc
    22501 tgtctgatga atatcgtagc cgatcacttt ggctcctagt cccttaaaca aaacagcttc
    22561 cgtcaaccct atcctgcctg ttcctatgat ccctactgtg caatttctga tttcacgact
    22621 aaacatttgc tcatccactc gaaaatcttt gaaagatgtt cggatcgtcg tatacgcagt
    22681 atttctcaaa agcatcatgg caaaagtcaa agataactct gctatcgcat ttggcgaata
    22741 cgatggtaca cgggctactg tcataccaaa atcagctgcc gcttgtagat cgatatgatt
    22801 gactcccaca gtccgtgtaa aaacataacg aatcccatat tcctgcattt ttgcaagatt
    22861 ggtctcatct gctatacaat ttcccctcaa caatacagca tcatggtcct ttgctgacac
    22921 gatattgtca tgtgtcaaca gttcttcata aagactgaga tcataatggt aaatattcaa
    22981 tttttcaaaa aaatctatct cgttcggacg aacaccgtaa catgcgattc tcatactttc
    23041 ttcctccatt tcttatgaaa ataaacctat gccatgaatc atttgtaata caacgatcca
    23101 tacaggtgtc agtaccacag caaaaatagt tgacagtaag gatgcattgg aggctaaaag
    23161 tgcctcccta tcaaaactga tagcataggc tgctgccact gttgcagctg gtgtagccat
    23221 catgataacg attgttgcca gtgcttcaaa attgactggt agaatattcg ttgccgtcaa
    23281 aactgctaga atcacgatat tcaatgcagg tacaaatacc actttgacta gactgtaata
    23341 ccatgaagtt ttttcagatg cagctttttt gaagcttact tcacctaatg ttgcgccgat
    23401 tgctagccac gccaatggtg aacttaaacc agctaaataa tccatcggct gaaatagcca
    23461 cggtgcagtc tggtcaattc gcaaaaacgc tacttggctt atctctcctt ctgcacctgt
    23521 aactgacact tgtggcagat acccttggaa cagccaaatg aaaaggccgg caaaagtagc
    23581 gataatgatt ggattgacga acatcgcttt gatattttta gcttccatct tcaagccact
    23641 catttttatg tatccataag aatacaaaaa gattcggtag ccaatattga aaattgagga
    23701 atacatcacg ccgacaggac cataaatcgc actgacgata ggcgttccga aaaaggtcgt
    23761 agaaccaaag atcgtcagga cacgcaacgt tgtttgctta tcccctttat agcttaagaa
    23821 aaaaggtttt gaaactagaa taagaataat ataaataact aatccccaaa tcaagacatt
    23881 gattccttgc atcagtaatt ctgtattgat atcttgcatg aaagctcgaa atgccagtgc
    23941 tggcaaagcc actgttaaga cgactttcga tagaattttt ccaacagatt cagaaaaaat
    24001 cccctttttt ctacaaacaa accctaataa tataataaaa actgttgaag tgattgcact
    24061 gacaatcgac atatctgtca aagtcgatcg aaaaatgtca aataagttca tctttctcct
    24121 cctaattatg ttcatttatt cacaataaaa aaagctttta ttttgctaat acgtattaac
    24181 atttttaatt ttatctttac attgttactt attgaacaat aggaaattaa agaaactttt
    24241 gaaacttatg taaagtgatt tttactagaa cacaaaaaaa accttaaaac caaacttaag
    24301 aagtttagtt ttaaggttaa cttaaattat attatgcgtg gaaacgttcc acgccatcta
    24361 gataagttgc ttccaatgtc atatcagggt tcaatacgat aaagtctgca tcgcgtcctt
    24421 gagcgatttt tccacatgta tcatcgattt tacagctgat tgctggaatc agactagcca
    24481 tcatgactgc ttgttctggt gttgctaatc cccagtctac aacattttta attgcttctt
    24541 tcaatttcaa tatacttcca gctaagttgc cagattctag acgtgctgtt ccttctttta
    24601 ctactactgg aaattcgcct aaattgtaat ttccatcagg catcccaccg gccatcatac
    24661 agtctgtaat taatgcgaca tgatcatgtc ctgctttttc cattaggatt cccgcagcat
    24721 ttggatgtac atgatggcca tcacaaatca attcagaaaa gacatgttct aaagataata
    24781 acgcacccac catacctggt tcgcgatgat tcaatccacg cataccattg aaagcatgga
    24841 caaatacact tgcgcctgct tcaacagctt tttgcgcttc ttctagtgta gcattactgt
    24901 gtcctagaga aacaactact ccttcttcag tcacttgttt gacaaactct tctacacctt
    24961 gacgttccgg tgctaaagca atttttttga ttaaaccacc tgaagcttct tgccattcat
    25021 ggaaagtatc caaatcagga tccccgaaat aacttgggtt ttgtgcccct ttgtgttctt
    25081 ctgtgaagaa aggaccttca aaatagattc ctttgatttt agctcctggt gcttcctgat
    25141 acattttgcc gattgtctca gctacatctt ttaaacgttc tttgcttgac gtcaatgttg
    25201 ttggtaaaaa tgaagtcact ccacaagata acagaccttc agacatcact ttgatgcctt
    25261 ctgcatcatt atccattaca tcatgattca tatagccgtg aatatgtgta tctaccaaac
    25321 caggagcaat ccatttgccg ctttggtcaa tgatgttgac ttctccctca ggtagttctt
    25381 tggtgtattc accaaaaagt ccatcggtaa tttcaagata gcctgcatgt ttgacacctg
    25441 acgctaaaaa gaatttgtca gcatagataa aagttctcat tctattctct ccctttgttt
    25501 ttctacctct aaagtactct tatttagaga agaagtcaag aatggtctac accatttaca
    25561 aattttttga aatttgtttc acagcttgtg ctaagccttc atagaatgct gctctttctg
    25621 gttcttcttt acgcttgtat attaagcttt taagcacaaa taaatcattg aggtaatagt
    25681 agctattctt ctcttgagcc caatagattc cttgattcac ctgttctaac gcttcgtcga
    25741 tttcattttg atggaataag aaatttccat aattataaaa aatttgcgaa aattcacttt
    25801 ttgatcgttc atcagctgat tgatgcagca gttggatgct tcgactcaaa tagtagcgtg
    25861 cttcagccgt ttttcctgcc acaccatata tttttccgat acaactaatc agttgtattt
    25921 ctgtgtcaga aacatggatt ctttcttttt ggctagagta gcttaatgct tctttcaaat
    25981 aataaaggga ctgagagata ttttgacgca acgtgaattc gcaaacaccg agataataat
    26041 aatagcgttg aaagtcagtt gcttgataca gattttcaga aatatcagaa gattttagta
    26101 cttgttctaa cttccgataa tcacgttttt ggaaacaact atccaacagt tcaaataatt
    26161 tatcgtttgt ccgagtagtt tttcgagaga gattcatcac atgatcaatc gttaccccta
    26221 gccgttgaca aatctgcatc atcaccaata cattcggcag ttcctcatta ttttcgatcc
    26281 gacttaatac actttgtgaa caaatatcat gcgaaagcat tttttgcgtc attttttgtt
    26341 cttttcgaat ctctttaatg atcgttccaa aggactcgat taacgtttcc ataagtatcc
    26401 tccccaaaat atgcgtattc gcattctatt cgtattttac tttaggaagt cgcaaacttc
    26461 aagaaaagac ttgcaactaa ataatatact ttagttattt aagaaaactc cactagaaag
    26521 agagcctcag tgtgtttgtc tgtggccctc ctactgaaaa atttggtttt taaaatcatt
    26581 tttggcgata ggttgctagg aattctctca ttttcttagc tgtttgtttc aaatcatcca
    26641 ttttaggaag ataaacgatt cgaaaatgat ctggctggtt ccaattaaat ccaccaccat
    26701 gtacaagtag aatatgatgt tcatgtaaaa agtccaaaac gaatttttca tcatccaaaa
    26761 tattgaatcg ctttgtatca atttttggga atatataaaa agcagctttt ggttttacgg
    26821 ctgaaagacc aggaatatca ttgattgcat tatagatata ttctctttgt tcatagatcc
    26881 ttccgcctgg caggagcagc tcatcgacac tttgataacc gcctagcgcg gtttggataa
    26941 tttgctgaga caatacattg gaacacaaac gcatggagga caacatattc agcccttcta
    27001 tatagccttt gacgtgtgac ttatctccac ttaggaccat ccagcctact cgaaatcctg
    27061 ctacacgatg agatttggat agaccgttca gcgtaacaac gaacaaatca ggcgctaatg
    27121 tagcaatcgg aatatgttct agaccatcca tcactaagcg atcatagatt tcgtcggaat
    27181 aaataatcag gttattttgt cgagctactt caacgatttc ttctaataat tcttttggat
    27241 aaagtgcccc agtaggatta ttaggattaa tgataacaat agcttttgtc cgactcgtta
    27301 tttttgattt gatatcatca atatcaggat accattctgc ctcttcatcg caaatataat
    27361 gaacaggtgt gcctccagct aatgcaatag aagccgtcca caacggatag tccggcattg
    27421 gaaccagtac ttcatctcca ttgtccagca acccctgcat acacatcgtg atcaattcgc
    27481 tcactccgtt gccggtgtag atatcattga tcgtcacatt cggaaaatgt ttcagttggc
    27541 aatattgctc aattgctttt ctagcagaaa agatcccctt ggaatctgag tagccttctg
    27601 agtcacgggc attcatgatc aaatcacgaa tgacttcgtt tggtgcttca aatccaaaag
    27661 gtgcgggatt tcccgtgttc aattttaaaa tactaatgcc ctcttcttgc atccgctccg
    27721 cttcttctag aacgggaccg cgaacatcgt aactgacacc ttctaatttg ttggattttt
    27781 caaattctct catcacttag ccaatccctc atttcaaaaa gttagaactt tccaaatttt
    27841 ttgttattta ctatttcaat atcatactcc atcctagaaa attttgccac ttatttagga
    27901 aaaacgtaag tattaaaaaa acacaaacaa gcgttgagaa cttaattgtc atgatagtca
    27961 ttccatagat tcgctgccca atccattgca attccacaaa tgattcctag cataaaacca
    28021 agcaaaaaac gtccgaatgc aagtccaatc aatacaccta agatcattcc cagtcctgaa
    28081 aattctccgt catatttctc catcttaata agtcctccca tttttgagct tgacaactat
    28141 gtaaaacctc gctatcatta gtgtgttatt attacgcaga tgtgttggaa ttggcagaca
    28201 agcatgattg agggtcatgt gagcgttagc tcgtgtgggt tcgagtccca tcatctgcac
    28261 tacaactcgt gaagttaatt ttgcttcacg agtttttttg tttgaacgtt tctttatgcc
    28321 attatataca tatcactgta tcctttacaa aaagaacgta caatcatgta aacagcctga
    28381 ggtgagtata tgaaggtcgg aaacaagcta ctcgtagaaa cttgcaagtg ttaaaacaat
    28441 taatcgaaaa acgagtaaaa tccctcacct aatactaaag cgagggattt tttgtatatt
    28501 atctgtttat caccgttatt ataaaggtac aaataccaag cacggttaat ccaaattgca
    28561 taaattcatt tttttgtaca ctcatgcttt tcattagctc tttaactgcc attagtacta
    28621 agatggcgat aacaacataa agtatatagt acgtccgcat tccggaagcc catactgtgg
    28681 gagaaaatcc cataattaac cttgaacaaa atcccattat catcaaaata actaaaaaaa
    28741 tagaagtttt cttattgtca aaacttaaat aaattccaac aattaaacat agtaataatg
    28801 ccaagactac taaagtagct atccaagtac ctggataaaa aagtgacagt ttcgttccta
    28861 actctgtaaa gagattattc aggttactac tactccaaat cattgccctt ttatttccat
    28921 tgacgtaagt aaagctttga cccattgtat taccaaaata aataatcagg tttaagaaaa
    28981 acggtaatgc agtcaatata cgcacataat agttttgaca tttcctatat gttagaagga
    29041 aaattaagaa aaatagcaat aaaaataaga tattcatgtc taaaaaaaac ggctttccaa
    29101 atgatgaaat acctaaatct aacttattaa gaaagttgaa atttttatat tcaggaaacc
    29161 atttattaat ttcatgtgca gcacgtaatg cattccctgg agttgtcaaa gagaaaatta
    29221 attctgctaa gcttattatt gaaaatactg ataacttata attatatcgt ccattgacaa
    29281 tcaaatataa actaactata cttgttagaa caaaaaaaca ggcgttgact tgttcttgat
    29341 tagttaaaaa tatcaataaa ggaatactta cccaatatat ttttctattt atttcttctc
    29401 ctcttaagag ttgaaaaaac ggataaaaag ttagtaaact aaatgctact ggccactgat
    29461 agttcaaggt ggttgctctc caaccggttt cccccattac tgtcaatggt attaaacagt
    29521 atatagagca aaccaataat aaatttttaa catttatttt ctcaaaaaca ta
//
LOCUS       DACGLW010000028        29105 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.028, whole genome shotgun sequence.
ACCESSION   DACGLW010000028 DACGLW010000000
VERSION     DACGLW010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29105
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.028"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            complement(<1..1028)
                     /locus_tag="IXT53_001275"
     CDS             complement(<1..1028)
                     /locus_tag="IXT53_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ4905115.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1110..1799)
                     /locus_tag="IXT53_001276"
     CDS             complement(1110..1799)
                     /locus_tag="IXT53_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ4905116.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1905..2273)
                     /gene="rplL"
                     /locus_tag="IXT53_001277"
     CDS             complement(1905..2273)
                     /gene="rplL"
                     /locus_tag="IXT53_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HAQ4905117.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2326..2829)
                     /locus_tag="IXT53_001278"
     CDS             complement(2326..2829)
                     /locus_tag="IXT53_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HAQ4905118.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     gene            complement(3239..3928)
                     /gene="rplA"
                     /locus_tag="IXT53_001279"
     CDS             complement(3239..3928)
                     /gene="rplA"
                     /locus_tag="IXT53_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HAQ4905119.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4032..4454)
                     /gene="rplK"
                     /locus_tag="IXT53_001280"
     CDS             complement(4032..4454)
                     /gene="rplK"
                     /locus_tag="IXT53_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HAQ4905120.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4797..5465
                     /gene="sdaAB"
                     /locus_tag="IXT53_001281"
     CDS             4797..5465
                     /gene="sdaAB"
                     /locus_tag="IXT53_001281"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit beta"
                     /protein_id="HAQ4905121.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5478..6350
                     /gene="sdaAA"
                     /locus_tag="IXT53_001282"
     CDS             5478..6350
                     /gene="sdaAA"
                     /locus_tag="IXT53_001282"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HAQ4905122.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6441..7460)
                     /locus_tag="IXT53_001283"
     CDS             complement(6441..7460)
                     /locus_tag="IXT53_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAQ4905123.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(7633..8520)
                     /locus_tag="IXT53_001284"
     CDS             complement(7633..8520)
                     /locus_tag="IXT53_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ4905124.1"
                     /translation="MTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEVSDKEKT
                     AESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDGQIQATG
                     DLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSMGGVSAL
                     RYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQDYQQMI
                     GNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTGENASHS
                     MLHENQEVDQLVSRFIWKE"
     gene            complement(8597..9142)
                     /gene="nusG"
                     /locus_tag="IXT53_001285"
     CDS             complement(8597..9142)
                     /gene="nusG"
                     /locus_tag="IXT53_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HAQ4905125.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9243..9413)
                     /gene="secE"
                     /locus_tag="IXT53_001286"
     CDS             complement(9243..9413)
                     /gene="secE"
                     /locus_tag="IXT53_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HAQ4905126.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9437..9589)
                     /gene="rpmG"
                     /locus_tag="IXT53_001287"
     CDS             complement(9437..9589)
                     /gene="rpmG"
                     /locus_tag="IXT53_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HAQ4905127.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9751..10395
                     /locus_tag="IXT53_001288"
     CDS             9751..10395
                     /locus_tag="IXT53_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ4905128.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10451..10954)
                     /locus_tag="IXT53_001289"
     CDS             complement(10451..10954)
                     /locus_tag="IXT53_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAQ4905129.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10967..11013)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11551..12516
                     /locus_tag="IXT53_001290"
     CDS             11551..12516
                     /locus_tag="IXT53_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HAQ4905130.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12615..13787)
                     /locus_tag="IXT53_001291"
     CDS             complement(12615..13787)
                     /locus_tag="IXT53_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ4905131.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13774..14919)
                     /locus_tag="IXT53_001292"
     CDS             complement(13774..14919)
                     /locus_tag="IXT53_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ4905132.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14933..15865)
                     /locus_tag="IXT53_001293"
     CDS             complement(14933..15865)
                     /locus_tag="IXT53_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4905133.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15982..17061
                     /locus_tag="IXT53_001294"
     CDS             15982..17061
                     /locus_tag="IXT53_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HAQ4905134.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17058..17687
                     /locus_tag="IXT53_001295"
     CDS             17058..17687
                     /locus_tag="IXT53_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ4905135.1"
                     /translation="MITVVIIDDDPFVTKSLQTILESTKEIRVLGIGHCAKDALVLYE
                     THRPDVLLTDIRMPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYL
                     IKQNLQAIIPSVKSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKE
                     VAAGKNNKEIADALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(17797..18024)
                     /locus_tag="IXT53_001296"
     CDS             complement(17797..18024)
                     /locus_tag="IXT53_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ4905136.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18127..20715)
                     /locus_tag="IXT53_001297"
     CDS             complement(18127..20715)
                     /locus_tag="IXT53_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HAQ4905137.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20735..21394)
                     /locus_tag="IXT53_001298"
     CDS             complement(20735..21394)
                     /locus_tag="IXT53_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ4905138.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21443..21946)
                     /gene="trmL"
                     /locus_tag="IXT53_001299"
     CDS             complement(21443..21946)
                     /gene="trmL"
                     /locus_tag="IXT53_001299"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HAQ4905139.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22167..23033)
                     /locus_tag="IXT53_001300"
     CDS             complement(22167..23033)
                     /locus_tag="IXT53_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ4905140.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23142..23783
                     /locus_tag="IXT53_001301"
     CDS             23142..23783
                     /locus_tag="IXT53_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HAQ4905141.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23845..25206)
                     /gene="mgtE"
                     /locus_tag="IXT53_001302"
     CDS             complement(23845..25206)
                     /gene="mgtE"
                     /locus_tag="IXT53_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HAQ4905142.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25224..26123)
                     /locus_tag="IXT53_001303"
     CDS             complement(25224..26123)
                     /locus_tag="IXT53_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HAQ4905143.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26125..26922)
                     /locus_tag="IXT53_001304"
     CDS             complement(26125..26922)
                     /locus_tag="IXT53_001304"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HAQ4905144.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26900..27604)
                     /locus_tag="IXT53_001305"
     CDS             complement(26900..27604)
                     /locus_tag="IXT53_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HAQ4905145.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27735..28310
                     /locus_tag="IXT53_001306"
     CDS             27735..28310
                     /locus_tag="IXT53_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HAQ4905146.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28428..29105
                     /locus_tag="IXT53_001307"
     CDS             28428..29105
                     /locus_tag="IXT53_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HAQ4905147.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         8562 a         6169 c         5232 g         9142 t
ORIGIN      
        1 acagtattat ttcggttgct tgctgcttca cctagccctt gtactcgtgt ttgattggcg
       61 acttgaccaa attgattccc gacgtctaat aaaataatag ctaaaagcaa aatggccaaa
      121 tggcttccgc caaccaataa agcaataaag cttattgttt gcataacaag tcccatatac
      181 acgatttttc tttcagaata agaatctgcc agtcgtccca cgataggtgc agcaactgct
      241 ccagacaaac caaaaatcgc taatatccct gcttcgaatg ttccccaatt gtaggcggga
      301 ctgctgatat aaaaaatcaa cgtagaccaa aagatcgaaa atgttccaaa catgaaaaat
      361 ccactgactg ccgcttctct taatagacgt tgagaagcaa ctaaatgcgg caaactttta
      421 agtgaatcaa ggtatgaaac agctgtacta ctttgctgga cagaacgcgg cattttccag
      481 taaagcagaa gtgtaagaat tccgactgca cataaagcaa ataaatagat catccgccaa
      541 gaaaaaagac tggcaacaat accagatacc gtccgagaaa gcaaaatccc cacgagtaaa
      601 ccgctaagca atgtacccat gaccttccct cttgcttttg gtcccgctag taccgcaccg
      661 taaggaatga tgatttgcgg cacgatcgaa agtaatccta ttaaaaaaga agcgcatgca
      721 aataacgtaa acgaaggcgc aaaaaaagcg gccaacagag agacggcaga taacgctacc
      781 attcgaatga tcaattttgg acgatccacc acgtccccta aaggaactaa aaacagtaac
      841 cctaacgcat agcctaattg ggtcaacatc gtcaatatcc cgactgcact tgtgctaaca
      901 gataaactag ctgctacttt tgttccaatc ggctggatgt aatacatatt ggcaacaaca
      961 acgccacaag tgattgataa tagtaaaata agcgaagttg ttaaaggtgt tttttcatta
     1021 ctcttcattg atttccccca tttcctatct tagtttacaa cttatggaaa agattggaaa
     1081 tagaaaaata caactattcg ccacacttat tatttgtgct tttgttttcc ttgttttttc
     1141 ttgcgatctt tataaatgaa gacaactaac cctactagca cagtaacact tgcaaccaac
     1201 gtgactgcag ttttgttata tccgtgaaac agagcttctc caccacacgc atacaacaca
     1261 gacgaaggga tgactccaat cacgatcgac aatatgattt gctgtagagg caatttcatc
     1321 gcatctgcag caaaattgac catagaagat ggaataaccg gtatcatata gcctaacatt
     1381 acacctattt ttggatgttt catttgtcgg attgcttgca cccaatgatt cgtttcggtt
     1441 ttcttatcta aaaattttaa gcgatgcata aggaaaattg cagtgatgtt accaagagca
     1501 ttacccaaaa tgttgatgat cgcacctgtc agtggtccaa agctcaaacc agcaatgact
     1561 ccaactactg aagtaggaat acctggaaca gcacacatca ccgctgtcaa tacgatcaag
     1621 agtatcgatg caaaagtccc atgcgaacga atatgtgcga gtagctctgc cttactcgct
     1681 ctcggattta aaaataattg gatgtctgtc tgatattcta aatacaaccg atagcccact
     1741 accagtatca gaatgattcc caacgagatc aacatcaatc ggattatttt ctttttcata
     1801 tttcctcctg aaaaaaatct atattcatta aataatagtt taacataaaa acaaaaaagc
     1861 accgaaatcc aacataggat ctcagtgctt ttaaatagta aaaattattt aacagttaca
     1921 gatgcgccaa cttcttcaag tttagctttc aattcttcag cttcttcttt agatacgcct
     1981 tctttaactg gtgcaggagc gccatcaact acagctttag cttctttcaa gcctaaacca
     2041 gttgcttcac gaactgcttt gataacttta actttttggt ctccagctgc agttaattct
     2101 acagtgaatt ctgtttgttc ttcagcagca gcagcgccac cagcagcagc tacagctaca
     2161 ggagcagcag cagatacgcc aaattcttct tcaatagctt taactaggtc gttaagttct
     2221 aggatagttg cttcttttaa ctcagcaacg atgttttcaa tgttcaatgc cattataatt
     2281 tcctccgttt aattgttctt ttttatttaa tgctttatgc ggcaattaag ctgcatctcc
     2341 atcttctttg tctgccactg ctttgacagc gtaagccacg ttgcggactg gcgcttgtag
     2401 tacagatagc aacatagata gaagtccttc gcgatttggc aattttgcca atgctgtgat
     2461 ttcttccaat gaagagactt taccttcgat gataccgcct ttgatttcta atgcttttgc
     2521 ttctttagca aattcgtcca tgattttcgc tggtgcgact acatcttcgt tactgaatgc
     2581 aacagctgta ggtccagtga atacttcgtc catgccttca aggccagctt ttttagctgc
     2641 acgtgaaaga atagaatttt tgataacttt catttctacg ttagcatcac gaagttgttt
     2701 acgtaaacgt gttacttcat caactgttaa tccgcggtag tcaacgacta ctactgatgc
     2761 agctgctgta aatttttcgc ttacttcatc aacgattgct gctttttttg cgataattgc
     2821 ttcactcatt tttgagtttc acctcctgaa ttttgatgga agagtttttt gcactgaaaa
     2881 ggcaagtcac ttttatagtt actttgctca accagtatta ttcatcacct attcacttcg
     2941 ttcatagtga ttctactcgc tttgctcgaa aggaaactcc tttcgctttg ctcaaccagt
     3001 attgttcatc atctcttcac ttcgttcata gtgattcaca ataaaaaact ctatgccacc
     3061 gcagacatag agggactatt gaatatattc aataaacgtc ctcggtagga aattaaggct
     3121 tgatgccacc tactgtcttc ggtacaagtt aattattaac ctcaactagc ttagcatgct
     3181 aaactagttg agtcaagaat tatttttcag ttggatcaat caacattcta atatttaatt
     3241 agaaagaagc ttggtcaaca tggataccag gtccaaatgt tgttgtgatc gttacatttt
     3301 tcatgtattg acctttagct gctgatggtt tagcttttag caatacatca tggattgctg
     3361 caaagttttc aactaatttt tcatcgtcaa atgaaacttt tccgattggt acatggatat
     3421 ttccagcttt gtccacacgg taagttactt taccagcttt cacttcgttt actgcttttg
     3481 ttacgtccat tgtaactgta ccagttttag ggtttggcat taagcctttt ggtcctaata
     3541 ctcgtcctag acgacctaca gtagccatca tatcaggtgt tgcaacaact acatcaaagt
     3601 caaaccatcc gttttggatt ttttgaacca tgtcgtcgtc acctacgaaa tctgcaccag
     3661 ctgcttcagc ttcttttgct ttgtctcctt tagcaaaaac taaaacagtt tgtgttttac
     3721 cagttccgtt tggtaataca acagcaccac ggatttgttg gtctgctttt ttagggtcta
     3781 cgttcaaacg gtatgcaact tcaactgttg catcaaattt tgcaatgttt gtttcttttg
     3841 ctaaagctac tgcttcggca acaggataaa ctttacttga atcaactttt tttaatgcat
     3901 cttgcatttt tttgctcttt ttagccattt tggttcctcc ttgattgtgg ttataacggt
     3961 tgtacctccc acgtgttcta tatctttcaa tatagagcca actgagaagc tgctgtctta
     4021 gggtgtatag attattctac agtgatcccc atgcttcgtg cagttccttc aaccatgcgc
     4081 attgctgctt caacgttagc tgcgtttagg tcttccattt ttaattcagc gatttctctt
     4141 acttgatcgc tagaaacttt cgcaacttta tttttgtttg gttcgcctga acctttttcg
     4201 attttagctg cttttttcaa tagaacagca gctggcggtg tttttgtaac gaatgtgaat
     4261 gaacggtctt cgtatacaga aataacaacc ggaatgatca aacctgcttg atcagctgta
     4321 cgagcgttga attcttttgt gaatcccatg atattgatac ccgcttgacc tagtgcagga
     4381 cctactggtg gagctggagt tgctttacct gcaggaattt gcaatttaac gattttttct
     4441 actttctttg ccacgagaca tacctcctta agtccgtgat gtggttaatt ggagatgcat
     4501 atttctcctc ccacgtttta tctgatctga aaaaatccag acaaataatg tgcaaaacaa
     4561 tgcacactgg tacagtatag caaatttatt tgttttttca agattttttt ctgctatttt
     4621 tacattttgc ttgaataaac gctaaattta ttctaaaaaa acaacaattt ttcgttctca
     4681 tttcttcttt tatgttacac ttgcgctagc atggttcaac agatgaaagc aaacaataac
     4741 acggtttttt agacccttgt acaaagattc ctacataaca aaatgaggtg gagaaaatga
     4801 aagatttaag gtatagaagt gtctttgata ttattggacc tgtgatgatt ggtccaagta
     4861 gctctcacac tgccggagct gctagaatcg ggaaaatcat acgtagtata tttggcgaac
     4921 agcctgattc tgtagatatt tatctatatg aatcatttgc aaaaacctat cgggggcacg
     4981 gaacagatat tgcattagtt ggcggcctgt tagggatgga acccgatgat gaacgtttag
     5041 ctgattcttt gaaattggca tatgaagccg gtatggaagt cttgtttgtt cctaagagtg
     5101 aaaaagccga acatccaaac tctgtaaaga tgcttctaaa aaaaggaaca cgaaagcttt
     5161 cagtcactgg catttcgatc ggcggtggca atatccagat ttctgaattg aatggattca
     5221 aaatttcttt aagcatgggt actcctacct tgatcgttgt ccaccaagat gttccaggta
     5281 tgattgctaa agtgactagt atactttctg agaccaatac gaatatcggc actatgacag
     5341 tgacacggga atcaaaaggt gaaaatgcca ttatgataat tgaagtagat gatcctcaag
     5401 tagaagaaac ggtaaaaaga ctcaaacagc tgccaaatat cgacagcgta aattatttcg
     5461 attaaggagc taaaaaaatg tttttatcga ttgaagaatt agtagatcaa gcaaaagctt
     5521 atccaagtgt agcagaattg atgattgcag ttgaaatgga aatgagtggc cgttcccgta
     5581 gtcacatcat cgagatcatg gaaaaaaacc tctcagtaat ggaacaatca atcaacgagg
     5641 gaaaagaagg tgttacctcc gtcacaggaa ttaccggtgg tgatgcaaaa aaattagatg
     5701 attatttaaa tgaggcagac tttttaagtg gagaaacaat cttaaaagct gtacgcaatg
     5761 ctgtagctgt taatgaagtc aatgcaaaaa tggggttgat ttgtgctaca ccgactgccg
     5821 gaagcgcggg tgttgttcct ggtgtgctga tggctgtccg agatcggctt gatttgacac
     5881 atgaacagca attggaattc ttatttaccg ctggcgcttt cgggttagtc atcgcaaaca
     5941 acgcttctat cagtggtgca gaagggggat gtcaagcaga agttggttct gcaagtgcaa
     6001 tggcagcagc agctttggtt tgtgcaaaaa atgggactcc tcatcaagca gcacaagcag
     6061 tagccattac attgaaaaat atgatgggac tgatttgcga tcccgttgca ggcttagttg
     6121 aagtcccgtg tgtcaaacgt aatgctttag gctcttctca agcttttatc tctgctgata
     6181 tggcattagc gggtatcgaa agtgtcattc cgccggatga agtcgttgct gcaatgtacc
     6241 aagtcggacg tcaaatgccg tcgattttca aagaaacagc agaaggtggc ttagctgtta
     6301 cgccaactgc taaacgactt gcggaagaga ttttaaataa gagggcgtga taaagctcct
     6361 taatttcgag tattaagaga ttagttatct tcataacaaa aaacgaagag aggactaatt
     6421 gcccttctct tcgtttttta ttaatccatc aatttcgtat tttttacttc atcgatcgtt
     6481 tttgttcctg caagttgcat cgtgatcgac aattctttgt tcaggtgttc aaaaacagat
     6541 gttactcctt ctgccccacc tagattcaac ccataaatca ctggtcggcc aatcgcaact
     6601 aagtcagcac cacttgccag agctttgaag acatgctctc ctcgacggac accagagtcg
     6661 aaaatcactg gtacacgttt gttgactact tcggcaattt ttggcaatac ttcaaaggac
     6721 gcaggacctc catctaattg acgtccacca tgattggata cccagatacc atctgcccct
     6781 gcagagatag cgattactgc atcttctggc gattgtattc cttttacgat aactggaaga
     6841 tgggtgattt ctttgattgt tttgatgtca tctggtgtca agccttgttt tgcagctgca
     6901 taaatttctg cgattccttt tccttcgcca ttaccacttg cactttgttc gctatacgca
     6961 gctaagttag gcattggtaa tggaaattgg aattgattga tgacgtcttc ttcacggtaa
     7021 ccaccgagcg ttgaatctgc cgttaaaatg atagctttag cccctgcttc tactgctttg
     7081 ttcaaaatga actcattgaa tctgtcgtct ttactcatat ataattggaa aaactgcggt
     7141 gcgcctggcg ctgcatcggc tgcgtctttg attgtcgtat ttgcataagt gctgatggaa
     7201 aagattgatc cagcagcagc tactccttta gctgtatcag cttccccttt ttcatgtgcc
     7261 aatccttggg ccgctgatgg cgcttggata atcggtgtat ctaattcaat tccgaaaaca
     7321 gatgtatgca aatcggctga gtcgattccg cgtaatatcc gtggcatgat tttctttgta
     7381 ttaaaagaag tcgtattttc tttcatcgtc cactcatctt cagaaccacc gcgaatatag
     7441 ccaaaagctc ctgcttccat gcgctctttt acacgacctt ctagttaggc aatgtttaca
     7501 atatcgattg gatgttcttc attacttgta tgataagtca tagtagatcc ttctttcatc
     7561 ttcttgatta catataaatc atactaaact tacaaaaagt aagcaaacat ttactctcag
     7621 atacttttat cgctattctt tccaaataaa tcgacttaca agctgatcta cttcttggtt
     7681 ttcatgaagc atactatgag aggcattttc acctgtcact actttttcct ctatttcatt
     7741 tccgcgttgc ttcaacaacg catagaccgc taatgcactg tttaacggta ctgttccatc
     7801 actcaagtct gtttcatcta gttgaccagc aatcaagaaa aatcgagtgg ttataggaat
     7861 attaccgatc atttgctggt aatcttgata gcggttagaa acgacagcag gcccctttgc
     7921 taactcatct gtcagagact gcgcactatc atcgacaaaa tcattaaaag gcgcgccaat
     7981 tgctgttaat gtctgaactt gtggcaatga cgtatcctgt ccatacgttc ctaaatagcg
     8041 tagtgcacta actcctccca tggaatgccc cactatgttg actttatcta tgccgtagtt
     8101 ttctttcaga tacaaaagac aagttttgat ccattctgct tgattccact cgttattttt
     8161 attatcttca aacaatactt ggaccatcgg attgtctgct tttccagaca aatctcctgt
     8221 tgcttgtatt tgtccatctg ttcctactgt taaaaccaat tcttttactg cttgctgatt
     8281 tttttccaaa cgccgcaaca tccctttaaa ggaattttct gtcccgctgt agccgtggaa
     8341 aaaaagtgtt ggaatagtgg ttttttctat cactttactg gattctgccg ttttttcttt
     8401 atcagaaact tcagtagtat ttatcttcgt cgtctggcaa ccacttaacg caaacaaact
     8461 gataaggact aaagctgctg cagttgcacg tttgcataaa tttttgattg gttctgtcat
     8521 acacctcttc ctttccatat agaaaaaaga aagacttgaa aggccaatca tatgatcgac
     8581 atttcaagtc tctttattaa tcgattttat ctacttgatc gaaatcaagt tctgtactgg
     8641 tttcacgtcc aaacatatcg atgttgactt tcaatttttc tttttctccg tcaacttctg
     8701 ttacttgtcc ttctaatcca gcaaatgcgc cttcgatgat tttcacgact tcgccaactt
     8761 ctacttctag atcattttgt cgcaggctca taccgataga acgcaagata tgattgactt
     8821 cttctggaag taatggtgct ggtttgcttc cggctccgtg tgaaccaacg aatcctgtca
     8881 caccaggtgt gttgcggaca acataccatg aagcgtctgt catgatcatt tcaacaagta
     8941 cgtatcctgg gaatgtttta tgaacgattt ctttttcttt cccatttttg acttcagttt
     9001 ctttttcttc aggaacaacg acacggaaaa taaagtcggc cattttcata ctttgcgcac
     9061 gagattcgat atttgctttt actttgtttt catatcctga atatgtatgc aagacatacc
     9121 agtttttttc aaaagattcc attgaaggct cctcatttca ttgtaaaata aaaaaacctc
     9181 agtacacccg aagtttttct ttcgcttcat tatagcacta aaagtacgtt gtggctagcg
     9241 agttatttta aaatccagcc aaataagctt tggatggctg tatccattac gtaaaacatc
     9301 aatccgaaaa taatagaagt ttcgatgact actaacgtat ctttacggag ttgttttctg
     9361 cttggccaag ttacttgttt gatttcatct tttacgcttt ttagaaattt catgagacaa
     9421 cctcccttgc tactgtttat ttcgtttctt tatgtaatgt atattgatta caatatttac
     9481 aaaacttatt gatttccaaa cgttcgccgc tttttccttc actgactgat tttgtgtagt
     9541 tgcgagaccc acaaacggaa caagcgagtg ctgctttttt ctttgccatt tacttctgcc
     9601 cccttgctga gaatcagact tatacttcat aacagtatca cgtaactttt tacttgtcaa
     9661 tttgtgaaat aaactatcta ttgttttgga taaaattatg atatgattaa tttgaaatta
     9721 agctaaaatg agacaattgg aggtatagcg atgctattaa aaacacttgt atataagaaa
     9781 aaagatctga caaccgttac agagactagt acattagaag aagcattaaa aattttagaa
     9841 acttccggat accgttgtgt gccgatctta gattcttcag gtaatatttt ccgtggaaac
     9901 atttacaaaa tgcatatcta tcgtcataaa gcaaatggcg gtgatatgac gcttcctgtc
     9961 actcatcttt tgaaaaacgc tacaaagttc atttacctgg atacttcttt cttcaaagta
    10021 ttctttacga tcaaagagct tccttatatt gctgtccttg acagtgaaaa tcatttttat
    10081 ggtattttga cacatagtac gttgcttaat atgctctctc aatcttggag cgtagaacaa
    10141 ggaagctatg tactgacaat tgcttccaca ggaaagcaag gagatttggc tgctattgca
    10201 aaaatcattt caaaacacag cagcattgcc agctgtatta ctcttgatat cgaaaaagac
    10261 gaatacattc gacgtacatt gatcacgcta ccggctaaca ctgaagcgaa agtatgtgaa
    10321 acgatcgtca accacttaga gaaaaagaat tataaagtgg tagaaattga aaatttgcaa
    10381 aaccaagaag cataatcaaa aagcgaaaaa agaaaacagc taatctgctt tcttttttcg
    10441 ctttttataa ttagacacgc ttttcaaaat caagcgcgcg atctaaataa tacatcaatg
    10501 gtgctgaaac agccatgacg ataaactcac ttaatgcaag cgtgccatag gttgtccaaa
    10561 atgcttggcc acctgttgga actaacatat aagcaatcag tcccatgctg atggtaaaga
    10621 agatcgtgtt caatactaaa cgcatcttta catttttcac tttgttttgc aataaagctg
    10681 tcaggcctaa agaaagcaat gtttggccgc caccaaaaac cacatccatg ataccaaaac
    10741 caaagaaggc attatagacg atgaccccgc ctaaaactcc ccaaagcaat ttacggttaa
    10801 agacaactaa atgattcaaa ctttctgaaa tacgaaactg gatcggccct gaggatacag
    10861 gcgcaactaa aatagttaac gctagataaa gagccatgat gatgccattt acaacgatta
    10921 cggataaccg ttgtctgctt ttcgtttttt gcattttctc tcctcttttc tcctagtttt
    10981 tttaacgcgg gatggttgat gaaccgcggt tatgtgaaac aaacaataat tataacagac
    11041 ttttcattta tttcaatccg tcttatctgc ttttaaaaaa gcaaaagctt aaacaatgta
    11101 aacgtttgct aaaaatcgtg ggatatggta taaagtaagt gtaaggggtc ataagaattc
    11161 ccagtaaagc agacgaagta ggatctgcaa gaacagaatt gttgtgaaac ttaaataaat
    11221 aaaaaaggat gtgggaattt gagcaacctc ttactagaga acggtctagc aagcagctga
    11281 agatctaagt ggacatgccg aaccttggaa cttctcctta cgtaagtaat ggatcatgcg
    11341 cttgctaggc ttttttgtgt ttttcggtct tcggaccttt ttcattttcc tagacttctg
    11401 ctataataac caagaattaa ttttgtaaca gatttttttg aaaaaacaag tttctttcat
    11461 tcaaaaaata gaaaaagctt gctaattatt ggttaaccag caaacaggag tattaaaata
    11521 tcttgtttta ttgaatagca ggaggaaatc atgaaattcc attatcatat attgatcaat
    11581 ccatcggcag gcagcggaaa tggctataaa gtagcagaaa gaattttacc ggtattaaaa
    11641 aataagcata ttgactatac acttcattat agtgaataca aaggacacga tgctgagatt
    11701 gctgaaactt tggcaaaaga aacattgatt ccttggatag aagaagaaca gaaaacaatc
    11761 gatactttcc cccttttgat catcgtagga ggtgacggca ccttacatca ggtacttgat
    11821 actttctatc agatggaagt agaattccca gttgcttata ttccagcagg ttctggaaat
    11881 gattttgctc gaggggcagg ccttccaaaa aatcccaaaa aaggacttca gctgatttta
    11941 gcagctcaat caccagaaaa agtccacata atggcttatg aagaaaaaat aagtgaaaaa
    12001 aaaggaatag ctgttaataa tttcggtata ggattagatg cagctatcgt ccatgcgaca
    12061 aatcattctt ccacaaaaaa gcgattgaac aaatacaact taggatcatt ttcgtattta
    12121 ttctctattc tccgtgcttt attcactcaa aaaggatttc caattctggt agatgcagga
    12181 ggaaaacaat taagtttttc aaacgcattt ttatgtacag caacgaagca tccttttttt
    12241 ggcggaggaa tcgcgattgt tccaactgct gatgcgtcta aaccagtcat tgattttgtc
    12301 gtggtcgaac gaatcaattt ctttaaaatc ttgtggctca ttcttttact tatacagaaa
    12361 aaacaattga aatcgaaata tttccatcac tacacaacaa acagattacg tatcgtttca
    12421 acaacaccgc aacatggtca agaagatgga gaagaaatgg aaaaacgacc ttttgatatc
    12481 acaataacca caactaaaca gttgttttgg tgttaataaa agaggttttg tcagaagcta
    12541 catctggaac accgtttatt cggaaaaaag gactgtgaca cgacttttgt cacagtcctt
    12601 tttttactat gcagttaatt ctttgcccaa ttgggttgcc atcgatagtt tctttcttta
    12661 cccaaaataa attggatcat tgaaaacacc gcagcaaaag caactaagac acaaccattg
    12721 aaccaaaaag attctgcaaa tgtttgattg taactgagtg tttcactgat caagttatta
    12781 ttttgaacaa accaatatgt tggtgtgaat gccgctattt tgctaaccat ttctggcaag
    12841 tattctgctg gtacaaacac cccaccgata aagcaactgc ctgtgataaa aatatctttg
    12901 atcccaccgc tggtttctga gttttttacc aatgaagtga tacaagccga aaagctaatg
    12961 atcggcaata aaaacaagaa agtatttaaa atatgatacc ataccgttat atcccacgag
    13021 gtgtggatca ctaacagaac cattagaagt aagaagccac tgaacaacag cagcgagtaa
    13081 ctaatcaaac taaaggttac tttgcgattc aagtttcgtt tggtaatggg cgcactctgg
    13141 tttcgtttac tgatctcttt gcgattaaac gctagattga tgacgctgaa accgctaaaa
    13201 atggatagaa acaacccata agacaagaaa ttaaaggttg tgcccgtcaa cagctttttc
    13261 atccgctgac tatatcccgc acttagctca acttctccag taaccgagag tgccatttgt
    13321 gtctgttcca tagctgcatc gattgtttta tcttcttctt gaaacagaat cagtgtttgc
    13381 aaaaaggtgt tcacttgctg tgtaacaact gttttagaaa aggcatctgg gcgactctgt
    13441 atcttgatcg tcgccatctt tcctacttcg acttgttcgg taaagtctgc aggcagatgt
    13501 aagataatgt ttacacgatt aaaataaaga gcatcatcga tttctttttg tgaggttcca
    13561 tccaatgtaa cgactgtttc cttgtcattc aaatatttac gaaggccatc gactacaggc
    13621 gactcttctt cactaatcaa ggcgattttc gcatcttgca gagaggtatt ttcaacatca
    13681 atattttgcg caaaaaagaa tgtggcaaca acagtgataa ttacacctat cagtaaattc
    13741 gatttatttg ctttaagcaa tgtccaaatc gttttataaa gcgtcataac gcacccctct
    13801 ttccatccaa gcatttccta acgcaaaaag gaaaataaat ccgatcaacc atagaagatt
    13861 ggtataaaaa ctagccaaat cagtgtagaa ataaagttta tacaaacttt cgctgatcaa
    13921 gttgacgaga tttaattgac caacaatcgg aatatgaaga tcaatccaat acttcacttg
    13981 ttccgacccc atcataccag caaaaaagct catcgcaatc gtcactgaaa tcaagatgct
    14041 atccttttgc accagattca tttttggtat caaattacca accaatgtac ctaacaaaat
    14101 cgcacacaac gaccccagag cggtaacaat catcaaccat tgccaacgtt caccaaagtc
    14161 tacttgatag ataaaacgaa agacagctaa cataatataa atctctgtca aaacaatcgt
    14221 aaagcttgct gccaaattag cgattgaaac taacgattgc tttctaggaa tcaaggacaa
    14281 gcgaatacca tttgacgatt gatttgcttg ctgatcctgt acattgcgga ccccccacat
    14341 cgtcccgtag agaatcgcca ttccaatcag agtgaagaaa taaaaacttt tgaaactcat
    14401 attacgacct gcatgaactt cttgaaaaga tacttcttgt tcaaaaacag tcatgatggg
    14461 cgcgagatca acgttctcca cttctgccac tttactcgaa attataccaa tacgctgtaa
    14521 aaattgatcc atgacgtttt tcatcaacgt ctgactcatt cctgcttgac ccacatggag
    14581 cgaaacagat gaaccatcaa tgagataaaa accagccaaa tcatctgcat ttagcttttc
    14641 attcgcttct acttccgata gttcctgtac ttcaaataat ggtttatcat tgacttcgac
    14701 ttgttctaag actgtggtaa aattttccga aagcgctgtt tgatccgctt tgattacaat
    14761 tccagtatta atcgtttcaa gattgccacc ttgatcaatc tcgccaaagg ccgccataaa
    14821 catcaatcct aaaagaattg gaaaggccaa tgtccaaaac aacatggatt tatttcgcag
    14881 cagtattttt gtgcgatatt tataaaaatg ccaaaacatt atttcccctc ctctagtctc
    14941 tcaaatcctt gccagtaatc tctaaaaaga catcattcaa ggacgcttct ttggttgcta
    15001 tttttccgta actgatcttt tcttcttgta agattgtaag aatcggcaac aatactgtat
    15061 ttagttcagt agttgttact tggagctgac cttttttata agcgattcca tcagaagcta
    15121 ttgttcgctg aatcgctgac aacacatttt ctgggagtaa tgggatgtcg atcgtcaaac
    15181 gatccgccgt ccgtaccata tcttttagag attctttggt tcctttagca atcacctgtc
    15241 cttgatccat gatcacgatc tggtcgcaaa gaatttctac ttcttccatg taatgtgttg
    15301 tatagacaat ggttgctcct tgtcgattca gctccttgat actttctaaa attttattcc
    15361 gactttgggg gtctacagct actgtcggct catctaaaaa aattaaccgc ggcttgtggg
    15421 caatcccaca tgcgatgttc aaccgacgtt tcaagccacc actaagttct cctggacgaa
    15481 acgaaacata tttttctaat cccactaatt gaatcacctc ttcaatcaag gctttccttt
    15541 tctctttttc ggaaacatat aaaccgcaaa aataatcgat attttcttgc acacttagtt
    15601 cgtcaaagac cgcaacgtct tgaggaacga ccccaatcat tgccttgaca gcataatttt
    15661 caggagtcat tgttgtcccg aacaatttaa tggttccttt gtcataattt aacaaagaaa
    15721 gcatacagtt gatggcagtc gatttcccac tgccgtttgg acctaacaga cctaagactt
    15781 ctccttcttg tacttgcaga tcaaaatgat tcaatgctac aaaatctcca tagcgtttga
    15841 ctaatttatc aatttcgata atcatcatga aaaactcctt ccaaatttct tgttgatctg
    15901 actataataa agtgcaagaa aagaaactag tgacgattgg ctaaaccaca catgacattt
    15961 gtcacaatga aggagtctgc tatgcataaa tctcggctac tcaacgaaac tagctttcta
    16021 ctggttatct cattgctttt gctggtaagt caaggattcg ttgctatgca agttatctat
    16081 ttattgatcg ccgttttatt cactggtgtc agttatttac tgcctacaaa gccaaattgg
    16141 attttccaag ttattctatt tcttttatgt gcctttttac ctgatcttgt cctgtttctg
    16201 ccagcgacga tgcgagtgat cgcccctgaa gaaagccacc ttgattactt gtttttgtta
    16261 ggttttttgt tgatttcgac ggttggtaat catcccccca tcaaggtcgc ctttgtcgta
    16321 attttgctgt ttttttcctt ttacttacga aaaaaagatc aacgaacgac cgatcttgag
    16381 aaaaagtaca ttgtctccaa agatactcat tgggaaaaac aacaagagtt atcggaacaa
    16441 aataaagcat tggccctgtc acaagaaaca caattagcct tgcaaattgt ggaagaacgg
    16501 aaccgaattg cccgagacat ccatgacaat gtcggtcatc tgctgtcaag tgcgatttta
    16561 caaattggtg cgcttgaaat gatcaatcaa gaaccaaaac taaaagaacc attagaaaat
    16621 ttaagtgcca ccgttcacac tgggatggat cggattcgtc aaagtgtcca tgacctccat
    16681 gagacatcga tctcttttca acactcctta gagtttctga tcgctgattt tccttcttct
    16741 gttacgattg aaggaaactt gtttgaagga ttgaatgaaa cgcagcaaaa ctgcttctta
    16801 atggtcatca aagaagcctt aaccaacagt atcaaacata gtcatgccaa aaccgttgtt
    16861 ttacattttc gaacgttgcc ggccttttat cgcttgcaaa tcacgaatga tggcaccagc
    16921 ccacaaaaaa attctgctcc cggtattggt ttgaaaacga tgcgtcaacg tgttcaagaa
    16981 caaaagggac aagtccatat tttccaagaa aaccagcaat ttcagttaac gatcatttta
    17041 ccaagggagg gacaccaatg ataacagtag tcattattga tgatgatcca tttgtgacga
    17101 aatcgttaca aaccatttta gaaagcacga aagagatccg tgtgttaggc attggtcatt
    17161 gtgccaagga tgcgctagtc ttatacgaaa cccatcgtcc cgatgttttg ctgacggaca
    17221 tccgcatgcc taaacaaacg ggcatcgatg ctgccaaaga aattttggct aaatttccca
    17281 aagccatcct tttgcttttg acgacattca aagatgaaga atacatccgc gaagcatttt
    17341 ccatcggtgt caaagggtat ttaatcaaac agaacttgca agcgattatt ccttctgtca
    17401 aatccgctta taatggacag gccgtatttg gtaatgaaat catcgagact ttcactcaaa
    17461 tgatgaagaa taaaccgacg attgatagtc atgcttatgc ttcttttagt gaacgtgagc
    17521 ttgcaatcat caaagaagta gctgccggga aaaacaataa agaaattgca gatgccttgt
    17581 acttgagtga cggcactgtt cgcaattaca tcagccaatt actagaaaag ctagatttac
    17641 gggatcgcac gcaattggct atctattttt atcagcattt gcaataagtc ttatctaaca
    17701 caaaaaaacc tgaatgatgc tccttcgcat cattcaggtt tttttcacat cttctattca
    17761 attgtcccgc aaaccatttc ctcacgattg aaagaatcaa ggattcgcag ggctgccagg
    17821 attcgttgga ttgttcggcg tgttaggcgg aaaaataggc gccttgtcta acgttttttc
    17881 tagttttgat tcttcacgat cgtctcgttc tacatcagga ctttcattca atttgaattc
    17941 tttttccatc tcatctaact ttttaaagtc attgccagat tcttttgcta atttcgctaa
    18001 tttatcttcg gtttctttag ccataaagta acgcccccct tttgatttca gcttagcata
    18061 tcacactgaa aaaacaaaag aaaacgctaa ccaacgatca tttttagcta tttctcatct
    18121 ttaaaatcaa gcattttttg ccggtataaa atcgtaggga gaactgtttt ccataccaat
    18181 catcggatct actaactggt tatcgatcac ttctgctgta agaacgaaat ctttagaaac
    18241 agcaggttct acctcttctt tcttttcttc tccaacaaat gaagaaaaca gatctggttc
    18301 cactgtttcc attacttggt ttttcgtttc attcgtccca ctgtcatctt ctacaggtag
    18361 cttttctttt acaacccgtg gctcttttgt atagttccca tcttcaaatg gatcatttcc
    18421 gaccaaagaa tcttcataag ctgctagttt ggtccggaga gtgatattca ttttatcatt
    18481 atccatgatc cttgatttca acgtagtcat ccggcttgct gattcattcg ctacacaagt
    18541 gacataggct tgctcttccc ctaacaaaat caacagttcc ttactgcgag tcactgccgt
    18601 gtagagcaaa ttacgctgaa gcatccgctg atattgatgg accatgggca aaataaccat
    18661 cttgaattca ctgccttgtg atttatggat tgagcaacaa taagaaagag tgattttatt
    18721 ccattcgttt cttttataag tgacctcatt tgcatcaaac tggataacta attcatccac
    18781 tttatcttca gaatcttttg ccaagatgat acccgtgatt tctcccatat ctccattgaa
    18841 tacattcaat tcgggagtat tgactaattg gaggactttg tctcctatcc gataaacggt
    18901 gtcgttccaa tgtacttcct tttttccagg tttccctgga ttgaatatct cctgcatcat
    18961 tttatttaac gcatcgatcc ctgcaggtcc ccgatacatt ggtgctaata cttggatatc
    19021 ttgtgcacgg aatcccttat ttttcgcttt ttccacaatt ttagagatca agggttcaat
    19081 ttgataagca tcacttcgga aataggaacg atctttttga ttctttgtga aatcttttgg
    19141 cagacgtcct tctttgatct catgggcaag aggaataata ctagaaccat caccttgccg
    19201 ataaatctga ttgagttcca ttttgggaat ttgttcgatt tccaaaagat catggagtac
    19261 ttgaccagga ccgacagagg gaagctgatc tttgtctcct actaagataa cttgcatatt
    19321 cgtcggaatc gctttgaata atgtgttagc cagccatgta tcgaccatag acatctcatc
    19381 aacaatcaaa agtccgcctt ctaattcttt cgtactgact gaaggtgttt tttcccgtcc
    19441 attcagcccc agaagacgat ggatcgtact ggcaggcaga cctgtcgttt cgttcattcg
    19501 tttagctgct cggccagttg gtgctgcaag tagaatagga aaaatttctt gggtatattt
    19561 tgaaggatcc aattccagtc cgttcaactc tgcaaaaaga ttgacgattc cgttgatcac
    19621 agtagttttc cccgtacctg gtcctcctgt taaaataaat agaggggaac ggattgcttc
    19681 agcgatagct tgttcttgcg aaggtccata ctgtatgccg aatatttttt cgatacgccg
    19741 aatattcttc tgcacttctt tttcaggata atggatttct tttttacgac tgagaaggcg
    19801 ctggatcgaa ttaccgatcc cccattcaga aaagtacaaa ctgttttcgt aaagattagt
    19861 ctcttcttgt tgtatctttc cttcttcaac aagttggata attacagtag caacctgatc
    19921 aggctggatt tcaattgggc gacttgtttc taaaatacgg atcgtttgct gaagcaatgt
    19981 ctccgccggt acgtatgtat ctcctgtttg aactgcatgc tgaaaaatct gatgcaagat
    20041 agctgctcga attcttctcg gtgaatctgc tgcaataccg atttgttcgg ctagattatc
    20101 tgcacgtttg aacccgattc cttctatgtc ttcgactagc tggtacggat tttcttcgat
    20161 gatatccaaa gactcgttct tataggcctg gtagatactg aaagctaact gactaccgaa
    20221 cccgtaacga ttcagtccca tgatcacttg atccatcccg tggtttagcc gaatcgtctc
    20281 agcaagcatt tctctttttg gcttcgtcaa accggagatt tgttttaata ccgaaggatc
    20341 agcaatgatt ttttcgatag catcttctcc cagcaattct acaatttttt ctgctgtttt
    20401 cttcccgatt cctgggaatt tttcgctgga aagatacgct acgatcccat ttgcagttgt
    20461 tggcttttct tgctcatatc tttcgagttt aagctgcctt ccgtatttag gatgatccac
    20521 taattctcca taaaaacgat aagtctcatt ttcttgaata tcgccgaaac ttccggtgac
    20581 tacaatctct ttttcacggt aatcagctgt ggtttcactg atgtgtacca aaagcacttt
    20641 gtaaaaatta ctaggatttt gaaaaaaaat cgcggcaact gtaccaacga aataaggcat
    20701 ttcttctggc tgcatcggaa gccgcctcct tcttctatag taaaggttct ggtgctgttt
    20761 cagttcctaa aaaatctgtt tggttccata ctttcagttc atatggcaat aatctgcaag
    20821 gctcttcagt ctctaaaatc gtcaaactac tattgaacaa tccacccatt tcacgcaact
    20881 cacttagctc ttttcctgtc atccattgaa tggctgcagt taaagaagct ccatgtccta
    20941 cgaataaaag tgggccatct ttatgtcggg caaccgcatc tgctacaaca gtttcgatac
    21001 gtgtgattgc cgattcaatc gtttctccgt gaaaaggaga aggatcatac agatctaaat
    21061 gattacgcaa attcggcaga ttatccggat atttctggta catttcatcg atcgactgac
    21121 cttctaaagt acccagccct agttctctta gcttatctgt ataaaaaatc tctaccggac
    21181 gagcaagctc tttgttgata ccttctgctg tatcacgtgc tcgctttgct gtactggaat
    21241 aaattgcttt aaaaggaaca tattttacct ttttcccaaa tgctttgatt tcttcgtaac
    21301 tttgtggcaa aagcggagaa tcgccattgc tcccttgaaa acgtctttcc tgattccatt
    21361 gtgtcttccc atgtcgggta aaatatagtt ccatacgcag caaactcctg tctttctttt
    21421 attttactct ataataaact gtctaatcca gcttgtcatt ttcatagtga tgcgtcaatt
    21481 ctaaattagg gaatccttgc tggcgaagtg cttcatacac tatcattgca gctgtattcg
    21541 ataaattcaa tgagcggaca tgctcatcat tcatagggat gcgtaaacac ttttcttcgt
    21601 tttcacgcat aaatgtttct ggcaaaccgg ttgtttcttt tccaaataaa aagaagtgct
    21661 cttctccatc agcaaaattt tcgtcactat atactttatg ggcaaatttt gagatcaaat
    21721 gtagtttacg attacctaaa aatgttaaaa aagcttctaa attactgtgg taagtaatat
    21781 ttacatcatt ccaataatcc aaccctgccc gttttagatg tttgtcatca gttgaaaaac
    21841 ctagtggctc gattaaatga agcggagtat tcgtggctgc acacgtacgg gcaatatttc
    21901 cagtgtttgc agggattaat ggttcaaata aaacgatatg attcatcagg atcattcctc
    21961 tcatatttat gttcgtttct attttctgac aatttgattt ttgcataaaa aaaaacgtat
    22021 caactcggtg tcagtcactg cgagtcgtta cgttagtgaa gctcttctca ctaactttta
    22081 tcagaacagg aacctgataa aaaccaatga aaaaacaact tgttgctaat ataacaccct
    22141 caactttttt ttgcaacctc tactcattaa aaactgctag aaaaacgttt tctcttagtt
    22201 ccttttaata attctatgtc aattgtaatc ttcttcaaag gattcttata taagtctgct
    22261 ttgattcgct cttctacctg ccattctaaa ggtgacatgg ccaaaagatc tttttgattt
    22321 tcctctggta tctcaaattc ataggttaca cgctgacgtt cagttaattt cattgcttca
    22381 gcaaacttgg ctactacacg ttcattggaa taactctgtt tttcaggttg attcggataa
    22441 aaagcctgac gtagttcctt taaataattt tcttgtggca ccaccttcaa cacttctcca
    22501 tcttctgaga gtacacgttg gaattcttgg taatgagaag gcgaaaagat atttaaaacc
    22561 gcgttgaatg tttgatcagc aaacggcaaa ttggtcaaat cagctacaca ccaaaatgct
    22621 tcggcagatt gttctgtagc tgcgtagact ccttcttttg aaatatcaaa tccaacagca
    22681 ttttgaatct ttttcaattt tgaaagttca ttcaagaagc taccttctcc gcagccaaca
    22741 tctaatagcg agacttcttc aggtaaatgc tgcgcaatag tctcaataac gggctgatac
    22801 atgccagatt ggatcattct acgccttgga acgaacattc cttgatcgta ttctgtttta
    22861 accgcatggg ataaaaaata aagtgttcct tttttggaaa ggtcaaaacg gtgattattt
    22921 ttgcatatca agccattatc agactttttt acttcttctt ggcaaaccgg acagcggaac
    22981 agctgatgat tttctgccaa aaactggcgt cctcggtcaa ttttttttaa catagttccc
    23041 cctcatttct gttttatctt accgttagat gctcactttg gcaactgcta aaaatatgat
    23101 acaatgccta gaagaaagtg aaagataaag gagaagatta gatgatcaaa gaattttgcg
    23161 ctgaaaacta cacatctatt cctctagcaa ttgctagagg agccgatcgg atcgaattgt
    23221 gtgacaactt agcggtcggt ggcacaaccc cgagtactgg tgtgatcgaa gaaacgttaa
    23281 gttatgctgg ggaaaaagat atccccgtaa tgacgattat tcgtcctaga gggggcgact
    23341 tcgtctataa cgatatcgaa ttgaaaatca tgcatactga tttgattgaa gcaaagaaat
    23401 tagggacgga tggcgtagta tttggttgtc ttactccttc aggctggcta gacgaagagg
    23461 ccttagaact attgatcgaa acagcagaag ggttgcagat caccttccat atggcttttg
    23521 acagtattcc tgaagatcgg caattcgaag caatcgattg gctggtagaa catggtgttg
    23581 accgtatctt gactcatggc ggtcctgctg acaaaactat tgtagaaaat ttcccacggc
    23641 ttaaagaatt gattgcctat acagatggcc gcttaatcat tttgcctggt ggtggtgtca
    23701 ctgcagagaa tgcagcacat gtagctaaag aattgaacgt aagcgaagta cacggaacca
    23761 aaatcgtgga tcttcagtta taatcttata aacaaaaaaa catttgcaac aggcataatg
    23821 cttattgcaa atgtttttgt ttttttacat aaaatagccc ataaataaag aagcaatatt
    23881 gaaataaatc agtacactcg tcaaatcgct caaagtcgtg ataaagggac cgcttgctac
    23941 tgctggatca aatcctagac gatccattag catcgggatt aaactacctg caagattcgc
    24001 cactgtgatt gcaaatagca tagccatccc tatgacaaac cccaagataa aattatgttt
    24061 ccaaaaacct acgattagaa aaatcgtgat ccccgtaaca gctcctgtga caacgccggt
    24121 caagacttca gccaaaacaa gtcgaacaaa tccatgatct ttctcatcag aaatcgcaag
    24181 tcgacgaaca gccacagcta gcgactgggt tccagcatta ccggcagttc ctgtaatcag
    24241 cgaaatgaag actgctaaaa tactcgcttc acttaccaag tcttcatagt gactgattaa
    24301 agaagcggtc gacataccta aaaacaacag agtgatcagc caaggcaaac gtcgagacgc
    24361 tgcctttact ggattttcat taatttcttc cacattgacc ccggcaagtc ctgaataatc
    24421 gctagcagct tcatcatcaa taacgtcgat gatatcatcg actgtgacga ttcctaacaa
    24481 atgatcatca taatcggtaa caggaagagc taagaagtca taatctctaa atgtttgtgc
    24541 cacatcttct tggtcgtcgc ctacatgaac tgaaagcact cgttcgctca tcagatcaga
    24601 aatcattgcg tcgtcatcat tcacgattag gtcgcgcaga gaaataaccc ctactagctg
    24661 accttcttga ttaacgacat agatgtaata gattgtttca gctacatctg cttcgttctt
    24721 cagcacgtac atcgcagagc gaacagtttg attggctacg atcgaaacaa attcagttgt
    24781 catgatcgcg ccagccgttt catcctcgta atggagaagt tccttgattt cactggcatc
    24841 gtctgccgac atcaaactta aatatttagc aatttgcttt ttatccagcg tattaagcaa
    24901 gtcaacagca ttatctgtat acatctcaga aagcatatca gctgcatagc taggacgcat
    24961 ttcatctagg tattctttca tatgttcgtt gtcttcttca atcacatcga acatatccgc
    25021 taattcttta ggcgacagat aggaatagat cagctgcctg ttctcttctg taagagattg
    25081 gtagaattgc ccctgctcat agatatgcat ctctaaaaaa ctgtttctga atgcctgaat
    25141 attattttcc cttaaatctt gagtcaactg ggcaaatctt tcctctaatt cttgattttc
    25201 atccaaacca atctcacctc gccttattct tcaaaccgtc tcacaactgc ctccatgtct
    25261 gcggctaaag gctgcttgaa ctccagcaac tcttctgtaa atggatggta aaaacgcaga
    25321 tcataacaat gaagggcttg tctttttatt cccagatcca ttcgcccgcc atacatatca
    25381 tcaccgagca gggcacagcc aattgcggaa aaatgaactc ttatttgatg tgtccttcct
    25441 gtatgcagct ggatatcaac taacgcgatt tctgttagtc gcttctctat ccagtactca
    25501 gtctgtgccg aacgtccttc atcagagacc attcgcttca atagcgaaga ataatctcgg
    25561 gcgatcggca aatcgatatg tccatgtgcc gataaatctg aaagccttcc actgactaag
    25621 gcctgatatt ttttcacaaa cttcttttca cgaagttgca tatctaattt tgcgtgcgca
    25681 aatccatgct ttgcaaaaag catcaagcca gatgtatccc gatctagtcg cgtcaccaca
    25741 tggattactt ggttttcgta accttgtttt ttataataag cctttacacg attagccatt
    25801 gtcccatttg gatggtattg ggcaggaata gacgaaacgc ctgctggttt atttactacc
    25861 agaacgtgtt catcttcata cacgatatcg atcggtgttt catccaaaag tacggtttca
    25921 tgttctcctt cagcaggaat cacaatggtt accttatcat ccttagctag agaaaacaag
    25981 acattttcta cttgatcatt aactttgatt tgaccgcctt gaaattttat tttcgctaac
    26041 agaccctttg agatcccttt ttcttttaaa aaatatttta cttgctgaga atgatctttc
    26101 tcataatccc atgtaaactg catatcaatc ctcactaata aaggcatctt tcacacgatg
    26161 ccaaaaatgc atatgtcgat aagaagcaaa atggatccgt tcttctgcta ttcgataata
    26221 aattgagcgg atatttgctt ttgcgacatt caattgatca accgttacaa gataatcttc
    26281 tgtattttcc aattttattt ctagccattc atcatgggca atcacgatcg gagaacccaa
    26341 agtacgaaac actcgattat tcagtgaagc aatctctgcc aactgaaagg cattgatact
    26401 aggatggatc acagcaccgc cgacactttt attatatgct gtcgaaccag taggcgtaga
    26461 aatcgtcaat ccatctcctc taaaactttc aaacagctcg tttttgatgt atacatcggc
    26521 taccatcgta cgattggccc gtttaatcgt tgattcattc agtgctaaaa aatgctggtc
    26581 cggtgtttca tccaaataac tgatccgaac atccagcaag ggataactaa cactttgttc
    26641 ccggttcgtt cgtaagctgt ctactagttc ttccagctcg taatcccgcc aatccgtgta
    26701 aaaccctaaa tgacctgtat gcacgcccaa gaaacgcaca tcattcagac aatgactgta
    26761 gtgatggaac gccgacaata atgtgccgtc tccgccgact gagatgacca attctggctc
    26821 atgatcatct cgttgaatcc ctgcttgttc taaaagtgcg ttcaaacgtt ctgtcacttc
    26881 tatagtcttt gcttctttat tatgaacgat cgctactttc atcccctgcc tcctctcttt
    26941 cgctttcttt cttcaaaagc gttcgatagt agacatcatc tgattttcct cttccatggg
    27001 agaataaccg ctgtgcttct tggatttctt cacggatact ggacatctct tcatccaact
    27061 gataggcagc ttctgccgca cgctgaagtc gttcactcat ttcttctgga aaaacgcctt
    27121 gatatttata attcagcgaa tgctcaatcg ttgcccagaa attcatcgca agagtccgaa
    27181 tctggatttc cgccaaaatt ttcttctcac cgctgatcag ctgtacagga tactcgatca
    27241 ccagatgata agaacggtag ccacttgctt ttttatttgt gatgtaatct cgttcttgga
    27301 gaactttcat gtcttttctt ttacggatga tctcaacaac ttgatggata tcttctacaa
    27361 actgacacat gatccttaac ccggcaatat cagacatttc ttcttctaaa cgtcccattg
    27421 gaatatgccg taattgtgat tttgttacaa tactatcgac tggcttgact ctcccagtga
    27481 caaattcaat cgggacatgt cgattttgtt cacggaactg tttgcggatc ccccgcagtt
    27541 tcactttcaa ttctgataca gtttgttcat atggtgctaa aaatgcttcc caatctcgtt
    27601 ccatagctga tacctctact tcattaacat tcattgttta ttctaccata tatgcaaagt
    27661 tttttcgtac tcttgattta tgcattgacc aaaaaaagaa aattaggcaa aatagacaaa
    27721 aaggagtcga tcaaatgaca caaatgcttg aaatcgaata taaatctatg ctgacaaagc
    27781 aagagtacga gatattcatc gctcactatc aattaacaga taaagatttc cgtgtacaga
    27841 ctaatattta tttcgatacc gttgatgaac agctgaagaa acaaaattgt ggcctacgga
    27901 tccgtttcgt tggtcatcaa gctgaatata cattaaaaac acctgctgaa aaagggcgtc
    27961 tcgaaacaac ggataccttt ataacagaag aagcagaagc atttatcttc gaaaaacgtc
    28021 tgcctcgttc aggcgcagtt tttcaaaaac tttctgattt gaatatcgat cctgcttcat
    28081 taatccaaac cggaaagctt acaacaaaac gagctgaatt ccctattgaa gaaggacttt
    28141 tagctattga tgaaagctgg ggagacaatc tacacgattt tgaactggaa ctagaggtag
    28201 gggatgcatc tgtgggaaaa attgctttta taaatttttt aaaacgtttt tctttacctt
    28261 atcgtcctgc taagaacaag atccaacgta tgcttgaagc taaaaattag aaacctttca
    28321 ttttcacaag gcacttgagt tcattttttt acttttttct gttaagttat aggatgtgaa
    28381 agaatctagt cactagttgt atagaatcta ttttggggga gattcatatg atcgaaatct
    28441 atttatttgt taatccttta ggggaatact gtttcgaaat ggaacaacag ttattacaat
    28501 ttatcgaaga agagtacggg acaccatcaa aagaaaagat gcaatttcgc tttcttcctt
    28561 tggtcaattt gcagacaatt ggggatgtca tgcaacaaaa aggaatttct cagaacaatc
    28621 ttgaggaacg taatcactta ttctcaatga cttattctgc agcattggac tgcaaagcgg
    28681 cgcagtttca aggaaagaaa aaaggccgcc gttttttgat caaattgcaa gaagcagtcg
    28741 gctgtaatga cataccatat tctcaagaac ttgctgaatc catatttgaa gaaatcggcg
    28801 gcgatttgaa tatgtttaaa gaagatcgtc agtctgactt cgttaaagaa gtattctttt
    28861 ctgatcaaaa aattgctcgg gaaatgggca tcacaaaaca cccttctgca gtcgtttaca
    28921 attatgcctg cgaccgtgat tttggtgttt tagtagaagt aaaaggtaca agtagccttt
    28981 ggagaatcac agagctgtgt aaaacaacag ataactcata tcaagttttt aatggtgaac
    29041 atttacatac aaatgattct cagttccatt caagaactag ccatttacac ttgctttcta
    29101 attaa
//
LOCUS       DACGLW010000029        28174 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.029, whole genome shotgun sequence.
ACCESSION   DACGLW010000029 DACGLW010000000
VERSION     DACGLW010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28174
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.029"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            complement(122..946)
                     /locus_tag="IXT53_001308"
     CDS             complement(122..946)
                     /locus_tag="IXT53_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HAQ4905148.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(919..1599)
                     /locus_tag="IXT53_001309"
     CDS             complement(919..1599)
                     /locus_tag="IXT53_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HAQ4905149.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(1622..2560)
                     /locus_tag="IXT53_001310"
     CDS             complement(1622..2560)
                     /locus_tag="IXT53_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HAQ4905150.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(2805..3656)
                     /locus_tag="IXT53_001311"
     CDS             complement(2805..3656)
                     /locus_tag="IXT53_001311"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HAQ4905151.1"
                     /translation="MELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADHLFFE
                     EITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAGLGGG
                     STDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQVLPS
                     MPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMGNSLE
                     DITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGFCEEV
                     YLVRTLQ"
     gene            complement(3810..6383)
                     /gene="mprF"
                     /locus_tag="IXT53_001312"
     CDS             complement(3810..6383)
                     /gene="mprF"
                     /locus_tag="IXT53_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HAQ4905152.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLP
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            6524..7330
                     /gene="proC"
                     /locus_tag="IXT53_001313"
     CDS             6524..7330
                     /gene="proC"
                     /locus_tag="IXT53_001313"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HAQ4905153.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(7409..8965)
                     /gene="rny"
                     /locus_tag="IXT53_001314"
     CDS             complement(7409..8965)
                     /gene="rny"
                     /locus_tag="IXT53_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HAQ4905154.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(9376..10425)
                     /gene="recA"
                     /locus_tag="IXT53_001315"
     CDS             complement(9376..10425)
                     /gene="recA"
                     /locus_tag="IXT53_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HAQ4905155.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(10534..11775)
                     /locus_tag="IXT53_001316"
     CDS             complement(10534..11775)
                     /locus_tag="IXT53_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HAQ4905156.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(12015..12593)
                     /gene="pgsA"
                     /locus_tag="IXT53_001317"
     CDS             complement(12015..12593)
                     /gene="pgsA"
                     /locus_tag="IXT53_001317"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HAQ4905157.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(12802..13686)
                     /locus_tag="IXT53_001318"
     CDS             complement(12802..13686)
                     /locus_tag="IXT53_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ4905158.1"
                     /translation="MAFETIGEKLRQARLNKKISLDELQQITKIQKRYLEAIDNDDFD
                     QLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQPETVSTSRKAMHQ
                     EESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDTASSLKVEGSVEES
                     SSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITDATKPVTLEFTAVN
                     RVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNATIKANGESVPVNP
                     GENNQSPKNVNLTLQYAE"
     gene            complement(14464..15750)
                     /locus_tag="IXT53_001319"
     CDS             complement(14464..15750)
                     /locus_tag="IXT53_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAQ4905159.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(15743..17011)
                     /locus_tag="IXT53_001320"
     CDS             complement(15743..17011)
                     /locus_tag="IXT53_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HAQ4905160.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            17263..17724
                     /gene="mscL"
                     /locus_tag="IXT53_001321"
     CDS             17263..17724
                     /gene="mscL"
                     /locus_tag="IXT53_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HAQ4905161.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(17783..18229)
                     /locus_tag="IXT53_001322"
     CDS             complement(17783..18229)
                     /locus_tag="IXT53_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ4905162.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(18322..18567)
                     /locus_tag="IXT53_001323"
     CDS             complement(18322..18567)
                     /locus_tag="IXT53_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905163.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(18682..19260)
                     /locus_tag="IXT53_001324"
     CDS             complement(18682..19260)
                     /locus_tag="IXT53_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ4905164.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(19337..19867)
                     /locus_tag="IXT53_001325"
     CDS             complement(19337..19867)
                     /locus_tag="IXT53_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HAQ4905165.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(19871..20731)
                     /gene="rlmB"
                     /locus_tag="IXT53_001326"
     CDS             complement(19871..20731)
                     /gene="rlmB"
                     /locus_tag="IXT53_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HAQ4905166.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(20724..21119)
                     /locus_tag="IXT53_001327"
     CDS             complement(20724..21119)
                     /locus_tag="IXT53_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HAQ4905167.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(21116..22528)
                     /locus_tag="IXT53_001328"
     CDS             complement(21116..22528)
                     /locus_tag="IXT53_001328"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HAQ4905168.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(22525..23061)
                     /gene="cysE"
                     /locus_tag="IXT53_001329"
     CDS             complement(22525..23061)
                     /gene="cysE"
                     /locus_tag="IXT53_001329"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HAQ4905169.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     gene            complement(23383..24843)
                     /locus_tag="IXT53_001330"
     CDS             complement(23383..24843)
                     /locus_tag="IXT53_001330"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HAQ4905170.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(25013..26170)
                     /locus_tag="IXT53_001331"
     CDS             complement(25013..26170)
                     /locus_tag="IXT53_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HAQ4905171.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(26204..27574)
                     /gene="radA"
                     /locus_tag="IXT53_001332"
     CDS             complement(26204..27574)
                     /gene="radA"
                     /locus_tag="IXT53_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HAQ4905172.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(27637..28164)
                     /locus_tag="IXT53_001333"
     CDS             complement(27637..28164)
                     /locus_tag="IXT53_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HAQ4905173.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
BASE COUNT         7743 a         6203 c         4978 g         9250 t
ORIGIN      
        1 agtttttctt caaaaattcg agcattttat gaaaaaataa gaaaatagtt cggtatttat
       61 taaaataaaa agaagcctat aaaaaatttt tcgtttttat aaggcttctt ttctttataa
      121 tttagttatt ttttcttctg aaggcaacca atattcgttt gatgatattt acaatcagga
      181 acaaagcaat aaagactaat gtgattgttg ctcctggagg tgtatctaga taaaacgagc
      241 tagttagtcc tgacagcatc cctagaaatc ctacacaaac actgatagca atcacgacat
      301 tgaagctttt gcctaatcgc ataccgattg ctgcgggaag aaccatgatt gcggatatca
      361 aaagtgcacc ggcaatcggg atcatcacag caatagccac accagtaatc acattgaaca
      421 tcatcgacat caaatgaacg ggtagcccat ccacgtgggc ggtatcttca tcaaaagtca
      481 gaacatacat cggccgtttg aataaaaaga acaatatacc gatggcaacg aataagattc
      541 ctaaaaagat gacttgctcc catgtaatgg tcacgatcga accaaacaga taggattgga
      601 tacttgttgc tgaattccct ccgctcaaat tcatcaatac taacgctagc gccaaaccac
      661 cagccattaa aatggcaatg gagatttctg aataggttcg gtagatcgtt cgcagatact
      721 ccaatatcac agctgctaat acaacgacta gcatggtcgt aagtgtagga ttaaggttca
      781 aaaagaatcc taacgctacc cctgctaaag agacatgaga aagcgtatca gccatcagcg
      841 attgtcttct gatcactaaa aacacaccta gcatcggggc gattccggca atgaagatag
      901 cggcaagaaa cgctcttctc atgaattcat atgaaagcaa cgccattgag aatcctcctt
      961 tctcaccagt cggatctgtc tgtccgcata ctgtttgata tcttcatgat catgggtgat
     1021 cattagaact gatttcccat ggacatgcgc actatgtcgg agaagacgat aaaattcatt
     1081 tctggattct tcatccatac ctgttgttgg ttcatcaaga acaaataagt cagggtcagt
     1141 agcaaaaata cgcgctaaac tgattcgctg cttttgtccg ccagacaatt cacctattcg
     1201 ccgatttcgc atatcccaca tccctacagc ttctaaagca cgctgtacat gttgatgatc
     1261 tttttcagag agacgcttga accagcggcc tcttggatat cttcctgaac ggactaattc
     1321 gatcaccgta ctaggaaaac ctgcattgaa agatgcaact tgttgaggaa tataaccgat
     1381 acttaatttt tttccttctt gatttttctc agcaatcttt atcattcctt ttgaaggttt
     1441 cagtaatcct aacgatgctt tcaccaaggt tgacttggct gctccatttt ctccagttaa
     1501 aatgacaaat tcacccgcat ctacatgata agagacatct tctagtacag gttcatcatc
     1561 atagtaaaag gtcaaatcag atatttcgat ataacgcacc ttacaacctc cattctaaaa
     1621 cttaattgat actttctttt aaagctttca gatttttttc catgacagaa atataatttt
     1681 cgcctgcttt gatttgttca ttagtcaagc tttctaaagg attcaacact tctagcttca
     1741 ctcctgtttc attcgacaat gtctcagcta ccttcgaaga ggcattttct tcaaaataaa
     1801 tcactttgat gttgtttttc ttcacaaatt cttttagctc agctaagcga cttggtgtgg
     1861 gttcctgatc tggtgaaacg cccgaaatcg ctacttgttt taagtcgtat tgattcgcta
     1921 aataagcaaa agcagcatgc tgagtgacaa atgtgcggtt tgttgcatct tttagctcat
     1981 cggagtattt ttgatccagt gcttcaagct ttttgatata agcatcggca ttcttcgtaa
     2041 aagtttcctt tttatctgga tatttttcgc ctaattcttc agcaatcgtt ctgacttctt
     2101 ttatagctaa taccggatcg agccaaacat gtggatcgta ttcatgtgaa tgaccatgcc
     2161 cttcttctga ttcttcatgt tcgtctgctt cttgcgattc taaacggtcg attcctttag
     2221 ctgcttcgat catcaaggtc tgtttgctat ccactgaatc ctttaacgaa tccacaaaaa
     2281 attccatgtc tgaactatta tacacgaaaa catctgcatc ggaaatttct gctctttctt
     2341 ttgcacttgg ttcgaaatca tgcggctccg ttccagcagg gatcaaaagt ttcacgtttc
     2401 cttcatcccc tacgatctct tttgtaaaat catacatcgg ataaaaagtc gtaacgatcg
     2461 tcaaatcctc ttttttgtct gcttccttgt tcgtatttcc acatgctgca aacagtaaag
     2521 cacctatcat taccgtagct gtcaaaagat attttttcat ttatatgctc ctttcaatga
     2581 acaaatcgga attttacgat ttattaacgt gaactaatgt atcaaaataa aaaaacacag
     2641 tcaatagaaa tccatcaatt tctgatttaa gcatgtatat tcttgattaa atcgtaaaat
     2701 ggatgagttg ctgctcatat ctagccattt cactacaata aaaaactgaa gaataccatt
     2761 ttaggattcc tcagtttttc atcacctatt ttacttctta tattttattg cagggttcgt
     2821 actaaataaa cttcctcaca aaaacctttt aacccattat atactctctg cgctcttgaa
     2881 taatgttgac acaaggcaaa aacggttggt ccgcttccac tcatcaatgc agcatctgct
     2941 ccgtacttga tcatacgctc tttgatttga tggatgactg gatgccgctt tcccgttata
     3001 tcttctaaac tgtttcccat ttttttgatc atctgctgat aatcttgctc tcggatggct
     3061 tcagcaagtc cgtggatgtc ttgatgttgg atggcttgcg tatctacttc tctaaacact
     3121 gtacgtgtag agacactcat tcttggtttg accaacacaa cccagcactg tggcatagaa
     3181 ggtaatactt ggatttcttc ccctttgcca ccgacaaatg ccgtacttcc gtagacacag
     3241 taaggaacat ctgtaccgac ttccaaaccg atttctgcca gttcttctaa cgaacagccc
     3301 agattccata attggttgat ccctcttaga gcagctgcac agtctgtact tcctccgcct
     3361 aatccagctg ctactggaat attcttctta acaaagatct tgacaccggt tttgatattg
     3421 tatttatttt ttagcaataa agcagcttga taaacattgt tccgttcatc taccggtaaa
     3481 aatgctttat ttgtttctac aatgattttg tcttctgtta tttcttcaaa aaatagatga
     3541 tctgccaagt ctacgctcga catcaccatt tccaactcat gatacccatc ttgccgtcta
     3601 tataatacat ccagtcctaa atttatcttg gctggcgctt tttcaatcaa ttccatcctc
     3661 tcacccattc gaaaagtcta agtcgcaagt attctatcat agggaaaaat aaaagtatag
     3721 cttatgtctg cctagtggtt gattttgtca tcgtttcaac agctttcaca gaattttctc
     3781 ttcataatcc aaacaagttc tagtgattct tataattctg tgagcacttc ttctgccaaa
     3841 ctcggaacat ttcctttttc ttttcctatc agactgagta gctgcatggt agcaaatccg
     3901 aaattacttc ctttcctata agcaaaataa cgtccttccc aatgatcgac atattttaat
     3961 ttcttttcat aagcagactg gaaggaatag aaaaaattac cataattata tacgatatgc
     4021 atgacttttt cttgaaagaa agaaaaatct gtttctccaa cattagctaa tggagctaag
     4081 cctagattgg cttccgagta gcccattttc tcgtatacaa taaacagatt ggctaacaga
     4141 tagtcagcta attcctcaga ttctttaggt aagacccgca acaaatcata tcccgcctgc
     4201 ttcttcgtca tcggctgagc ggtgatgaag ccaacgactt tttgatcttt acggacaata
     4261 ccgacaccgc tagataatac atatgattta tcaaatcgtc ccacagagaa gtatttttct
     4321 cgtgcacctt gaagccattt ttgtgaaact tcatttacag cttgatgtaa ttcttccggc
     4381 agttgatcgt accaagtaaa atggtatcct tctttcatca aacgttgata ttcaagttgc
     4441 tgactcagcc gagacaagtc ttctgttgct agatcaacaa tccctgcttc acctactttc
     4501 gtgaagtgaa aacccaattc atgaagcatc actacgtagc tttctgatat cttataaaac
     4561 gcaagctgat agcctaaaca atctgcttgt ttcataaact gattcgttgc tgcccgcatc
     4621 attttaggat tgccaactgg atctcctaaa acaaagcatt tgtttccttt gatctgataa
     4681 ccaaaaacga cttgatcttg gtcgtcttct tggtaataaa aataactgta acctgacaag
     4741 cgaagctgat ggcttgtagc tgtgcctccg tatgtttcca ataaacggtc aaaacggtct
     4801 ttttcgtact cactgccaag cttcgtagta tcttcagcta aatattgata aagcgccact
     4861 aaagctagca atgagatgcc caaaccaata agccctgaga accacacatc atctgaagga
     4921 aacaaaataa aatgatttcc tgttattcga ccgactaaaa agcgtccgct gtgatagcct
     4981 gcaattgcgt aaacgataaa caaaaagccg aacaagctag catcaaagat tatagctccc
     5041 caagaataaa cgaattttgt tcgataaaat tcttttctgg ataaccacac agctaacaga
     5101 atcaagacat ataccaatat caactgccaa gataacgtac gtgtgattgt atttaaaata
     5161 ccaaaagata gtaaagcaat cgtaggcaga aatgcctttt tgacttttac tgaaagtgct
     5221 ctagcaaggc cgagtaataa aaagccaacc aatagattca ctgtctgatc taaaaaattg
     5281 aatgagaacg gcaataatac ttgaaatagc cgactgattg cagacagatt agtgattgtt
     5341 gatagaagta ccatcatgat ccccgcaaaa tagacagcac ttacgagcac gatatgcgca
     5401 accttttgga aaaacagtcg cggaatgtta tctaaaaatc gattgatttt gactcctgta
     5461 tgtgtgatga acaatagaag tcccgtcata aaaggtagaa cataataaaa caaccgataa
     5521 tataatagcc aaactactgc tacatcttgc cgaacaggca attgactcat tcctaagatc
     5581 atcaaaacgt caaaagtccc cattccacct gggaccatcg tcagcattcc tatcaatgta
     5641 gcaatcatga acataggata gatcgaaagc atcgaaatat ctattctcat caacttgccg
     5701 atgatcaaaa ataccgcaag ggctcccgtc cattgtccaa aggatgccag tagcagacca
     5761 agcaagcctt ttggataaaa ctcgaagaac aatgtctttc tctttagata agcaaaaaag
     5821 aaaatgactg gtgctaaaaa actaccgccg aaaagccaga tccaatagct tcgaaaaata
     5881 ctttctgact gaataaaaaa gacatcaata aatgtaagaa acgaccatac agacaaacca
     5941 gtcagcataa atagtgcgac ttttgataca gtggctaaaa ccatttttct atttgtctgc
     6001 tttccataaa aactagcccg cagacttgct cctaccacac caccaaaacc ggcgagattg
     6061 ttgatcgtgt ttgttaccca cgcagcaaga agaaactctt ttctttccat ttttggtttt
     6121 ccttgttttt ctaagactct gatcgtcacc caatcataca acaacatcgg agtgacaccc
     6181 agcagtccag ccagaatcat gagcaggatc gtcatgcggc tttgttgtcc catcctttgc
     6241 caaatatctt gccaactcat gccattagca atatttgcga cttgatttgc cacaaagact
     6301 aacacggagc cgaaaaaaat cagttttaaa atcagactat gttcctttat ccattgcagc
     6361 atcaagtatc gtttattttt cacattttcc cattcctaac ttgtatactt ttgtgtcata
     6421 ccccaatgtt atcttattta ttttaatttt tccactttag acctgttctt tgcttgcgat
     6481 gatagaaaaa gaaagatatc ctaatgctaa aggagaggat aacatgaaag taggatttat
     6541 cggcacaggg aatatggcac aagctattat tgatggttta ttaaaaaaag gaacagtctc
     6601 gaaagaagag gtgttcgttt ccagtggaca ttttgaaaat gcacaaaaat ttgctgctga
     6661 aactggcgtc catgcatgca aaagtaatca ggaagttgca tcacaatcag atatcgttct
     6721 tttagccgtg aaaccaaaaa tcattgaaaa agtattggaa gaactaagag aatatagcca
     6781 gaaagtctta ttcatttcta ttgcttcagg aaaatcatta acagatttag aaacagcgct
     6841 tgaagatcct caggcctcca tcgtacgtgt gatgccgaat gtcaatgtca gcgtaggagc
     6901 aggcgtttct gcgatttgca aaaatgaagc tgccacggag gaacagcttc agcaagctac
     6961 gatgattttt gaagcaatcg gcagcgtcta tcctttagca gaatcagatt tcagtacatt
     7021 cattggcctt gctggcagct cacccgcatt cgtttacatg ctgatcgatg cgatgggacg
     7081 gactggtgta ctgaatggtt tgtccaaaca gacagccact gagattgctg caaaagcaat
     7141 tatgggttct tgtcaaaaat tcctttcaag tgataaaaat ccatgggaac ttgtagacca
     7201 agtttcttct cctggcggta caactgttgc aggtgtagtt gccttagaag aagcaggatt
     7261 catcccagca atcatcaaag gaatcaatac gacgatcgag aaagatcttg agatgttgaa
     7321 taaaaaataa tgatagcaat gttttttcgt gccttacaaa aaaagaagag ctgtggcttt
     7381 tgccgcggct cttcttttgt attttttatt atttagcata atcagttgca cgagtttccc
     7441 gaataacgat tactttgatg tgtccaggat aatccagttc ttcttcgatt cgtttacgaa
     7501 tatcacgtac taaacgaaca gcgtctaaat ccgagatttc ttctggcttg accattaccc
     7561 gaacttcgcg tccagcttga actgcgtaac ttgattctac accggcgaag ctgttcgaaa
     7621 tattttccag acttcttaaa cgacggatgt agttttctaa tgattcactt cgcgcaccag
     7681 gtcttgctgc tgagagcgca tctgctgcag ctacaaggac agagataaca gaagtagctt
     7741 ccacatctcc atgatgagaa gcgatcgcgt tgattactac tgggttttct ttgtacttgg
     7801 cagctaattc cgcaccgatc tcaacgtgag atccttcaat ctcatggtca agtgctttcc
     7861 caatatcatg gagcaacccg gcacgttttg ctaattggat atcttcgcca agctctgctg
     7921 ctaagatacc agctaatttt gcaacctcga ccgaatgctt caagacgttt tgtccataac
     7981 tagttctgaa tcgcagacgt ccgatgatct tgattagatc tggatgaagt gtatgagcac
     8041 cgacttcaaa cgctgcctct tccccgtatt cgcgaatacg ttcatccatt tctttacgag
     8101 atttctcgac catttcttcg atacgcgctg gatggattcg tccatcttgt atcagttttt
     8161 ccaaggtcat ccgtgcgatt tctcttcgga ttggatcgaa tccggaaaga acaactgctt
     8221 ccggtgtatc atcgatgatt aaatcgatac cagtcagcgt ctcaagcgtt cgaatattac
     8281 gaccttctcg tccaatgata cgacctttca tttcatcatt cggaagggta acgactgaaa
     8341 cagtcgtttc tgaaacttga tctgctgcgc agcgttgaat cgctaatgat aataagtttt
     8401 tcgctttgcg atctgcttct tctttggcac gctgttcaga ttcttttacc attagagtta
     8461 actcatgatt cagctcttct tcagtcgatt tcatgatgac atctcgtgcg tcctcttttg
     8521 ataaaccagc aattttttct agttcttttt gttgatcttc aatcattttt tcaacgtctt
     8581 tttctcgctc ttcaattaat tgctgttttg aactaagtgc ttcttcttta ctttcaagtg
     8641 agttctcgcg tttttctaac gagtcatctt tacgattcag cgtttgttcg cgttgaatca
     8701 aacgattctc ttgagatttt aattctaact tactttctct cagctcactt tcaacttcag
     8761 aacggtattt ctgattttct tccttagctt ctaacaatgc ttcttttttt agagtttctg
     8821 cttctttttt tgcactatcg atgattccag cagctgatga ttgtgcacca gctatttctt
     8881 tctcgtgacg tgactttgcg accataaacc ctaaaccaag accgacaatt aaaccgatga
     8941 tagcgaggag aatgttgaat accataaaat ccacctccat actatctttt tcatttttat
     9001 catctataca gcttctatga taaattattc taaactcgtc aatatgcatc ttcgcatata
     9061 tgtgtacaaa aaacatgcac aactttattc taatgtttgt accccgtagt gtcaacttta
     9121 aataaacaaa gaaacggctt tctacttatt cattaaacga tggtttttaa aataaatgac
     9181 actagtttca tataggtaaa cctttaaatt gagaaacttt tctattaaat tattctttgt
     9241 agatgaacag atatttttat tttatgtacc cactcgtata attaaataac tcacgatttc
     9301 tttattcaat aaaaaaacgg caggtcaagg agagtaccta ctcttcagac cagccgtttt
     9361 tgtttatcat aatttttatt cttctactaa aggcaattct tcttgtcctt tttcatcttc
     9421 tgggacagcg acttcttcac caataccata agctgctcta actagagcag aaacttctgc
     9481 catcatttca ggatgattag ccatatagat tttagcgttt tcccgtcctt gaccaatccg
     9541 atcttcttta taagaatacc aagcaccgct tttatcaaca atgtcttttt ctactgccat
     9601 gtctagaagt tctccttctt gtgagatccc taaaccatac atgacatcta cttcagcgat
     9661 tttgaaagga ggtgctactt tgttttttac gactttgatc tttgtacggt taccaacaat
     9721 atctgtacct tgtttcaatt gttctgcacg acgtacttcc aaacggatcg ttgcgtagaa
     9781 tttcaatgcg cgtcctccag gagtgatttc tggattccca aacatcacgc ctactttttc
     9841 acggatttgg ttgatgaaaa tagcgattgt ttttgttttg ttgatcgaac cagagagttt
     9901 acgcaatgct tgagacatca aacgtgcttg taacccgaca tgtgagtcac ccatttcgcc
     9961 gtcgatttct gctcgtggaa ctaaagcagc aactgaatcg actactacga tatctacagc
    10021 cccacttgat actaaagcat cagcgatctc tagaccttgt tctcctgtgt caggctgtga
    10081 aagaagtagt tcatcgatat tcacacctaa tttttgtgca tattgcggat ctaacgcatg
    10141 ctcagcatca atgaaagcgg ccgttccgcc atttttttgt acttctgcaa tagcgtgtag
    10201 tgcaactgtt gttttacctg aactttcagg tccatatact tcgatgatac gtccacgggg
    10261 gtaaccgccg acacctaaag cgacatctaa cgcaagagag ccacttggaa ttgtagagat
    10321 ttgttggtcg attttttcgc ccaatttcat aatagagcct ttaccatagc tcttttcaat
    10381 ttttttcagt gcagcatcta aggctgcttt acgatcatct gccaatacga taatcctcct
    10441 tatatttaca aatcagagca acatttcatt atccttatca tatctttttt agtgaaaaaa
    10501 agcaagaaaa aaccgaacaa atattcgcat ttatcatttt cttaaaagct gttttctcaa
    10561 aagatcaaat ccttgcataa ccgcattata tctgatagca gcacgatctc tggcaaagtg
    10621 atatttttcc gaaaccacgc cgtctttaga tgaaatagcg atccatactg ttcctgcaga
    10681 ttgaccttct aacgtgtccg gtccagccac tccggtgaaa gctacactgt aatctgttct
    10741 tgctatctca cgtgcttgaa tggccatctg ctccacacat tctttgctga ctgttccata
    10801 ttctgctaat agctccggat caatctccag aaaaccagct tttgtttgga gcgagtaggt
    10861 aacgaatccg cctggaaata cttcagatac accagcaatg tcgcctaatg cggcttggaa
    10921 ggcgccggct gtcaaacttt ccgctgctgt tacagtctgc ttgttttctt tcagtagttc
    10981 cactacgact tttgctagcg aattatcatc gccgtatcca taaaaatatt cccctactcg
    11041 ttcttggatt ttttcttcta atgctaaaag agcttgattc cctgcctgaa catcatttgt
    11101 tttcactgtc agtcgtaatg tcacttcatt tggtttggca tatggtgcga tcgttggatt
    11161 gatctgtgtc tcaatcagat cttttagctc agtgaccagt cgcgattcac caatcccata
    11221 aaagcgcaaa actttagaaa ttagtttttc ttcagaagga aaacgttcct ctagtaaagg
    11281 acgtacttgt tccacaaaca ttggtttcaa ctcattaggc ggaccgggaa gaaggatata
    11341 cgcatgtgta tctgtttgat aaaaagtccc taaagcaagc cctgtacgat ttggtagagg
    11401 aaccccgcct tcgataatct gggactgttg cagattgttt ttggtcattt ttcgatgggt
    11461 tgtttcaaaa tatgccaata attttttgta tccttccgta ttttgtatga gagacttacc
    11521 gaggtgagcc gcagttgctt cttttgtcag gtcatcttct gtcggtccta atccgccaca
    11581 taagagtatg agttcacttc ttgtttcagc tagagcaatc aactcttcta aacgctgctg
    11641 attatctcct acaactgttt gataataaac ttcgatacct agatcagcta gctgttctga
    11701 caagaaagtg gcattcgtat tcacgacttg tcctagtaaa agttctgttc cgacagctat
    11761 tatttctgct ttcattactt cccgctccct tatatagtat acgctgtatt tttatatttt
    11821 tttatttaaa aagagggcaa ggagctgtga caaaaatctt gtcacagctc cttgttccaa
    11881 ataaacagtg ttctcctgcc aaaacctcgg tcataagtca caatatacat aaaacatgaa
    11941 aagctgtttt ttgaaattat cccttgtgac tcagagttag acggcaggct cacaacctct
    12001 ccactaaacg atacttacat tgatcctttg aacacaccgc tgtttttgat gaagtaatcc
    12061 acacctgaat agatagtaaa gatcagacaa gcataaagca tgatttgatc tactggaata
    12121 ccgatcaggc tgaatgggat attattgata aacagcaaga taatagcaac catttgtgta
    12181 gccgtcttga cttttcccgg ccaagctgca gccatgactt ctccatgttc gacaagtagt
    12241 aaacgcaagc ctgtgacggc taattcacga cagacaatga tcgctactac ccaaccaggg
    12301 gcctttcctt ggtcaaccaa catgataaaa gcagtcataa ctagcatttt atctgccaac
    12361 gggtccgcaa acttgccaaa attagtgaca aggcctcgcg cacgggcaat ttttccatct
    12421 aaccagtctg tgatactagc taaagcaaaa ataactgcac ctactagctg tgtgacagcc
    12481 agtgtggtgt ctcctacgtt aaattgtccc caatctaaag gtaaacctac aaccaaaatg
    12541 aaaatcggga tcatacaaat tctaagtaca gttaatttat ttggtaaatt cacactatcg
    12601 actcctttct tccctattct acgtgactat cagcgaagtt tccaccgata ttatttactt
    12661 ttccaaatga atttattcaa aggagtattt ttattgaaat tgatcttttt gaattaacga
    12721 agaaggaccg cgacacagat ttgtcacgat ccttcttttt tatacctaca agtaaacaga
    12781 tgcgtaaact aatttatacg atcattctgc gtattgaaga gtaaggttca cattttttgg
    12841 actctgattg ttttcccctg gattaactgg tacggattct ccattcgctt tgatcgttgc
    12901 attgcttgct gcaccaatcg tgatggtcgc atttgttgct gtttccggta acacggtact
    12961 ctgtgtttca tttgctgcca gtgtcccttg ataaacatag gcattgtcta ctagcacacc
    13021 taaccataca cgattgacag cagtaaattc aagggttaca ggcttggtag cgtctgtgat
    13081 atttactgcg atggcactgc cagaatcttc tcctgtagct attgccattt tcttttcttc
    13141 acttgatttt gttgttgatt ctactgtaga agactctttg gtctgcgtgc tttgctctga
    13201 ggaagaggac tcctcgactg atccttctac tttaagcgaa gaagctgtgt ctccgattat
    13261 tggatcagct tgacgatctt gccaagtcat atagcccacg acaacgacaa tcgccaaagc
    13321 aaccaatcct aataaaatga caggaagact tgtccaaaag cgacttggat gcgattcttc
    13381 ctgatgcatt gcttttctag aagtacttac tgtttcaggc tgcggacgtt cagggaccga
    13441 tcctgtcaat ttgcttttcc catcaaaaac atcgactaaa tggtctccat cttctcctac
    13501 agcttcagcg tattgccgaa taaatgcccg cacgtaaaat ttaccaggca attggtcaaa
    13561 atcatcattg tcgattgctt ccaaataacg tttttgaatt tttgtaatct gctgtaattc
    13621 atctaaagat atttttttat tcaggcgtgc ttgccgtaat ttttctccga tggtttcaaa
    13681 agccactcgt tcatcttctc cagtctgttt gattctccga agcatctgct gttgcaacga
    13741 aagacgttat gtttgggtgc tgtgacaaaa gtcatgtcac agctcctttt ttccgaataa
    13801 acattgttct cctgccaact cctcggtcac aagtcacaat atccaataaa tataagaagc
    13861 tgttttttga acttgttcct tgtgactcgg agttaaacgg caggctcacg acctctttct
    13921 taaacggtgt tcaatcagct gttcctcggt attagctcaa aaaccggcaa aatataagga
    13981 gatatttttc cgtatgtttc tctaatactc aggtcaaaac gctgatttca caacctcttc
    14041 cttaaacggt gttcaaaagc ttgcatctgc ggtaataagt ccaaccaagt taaaaacatt
    14101 aagaagctgt ttttacttgg ctgttcttat tacttgatgc agggcagctt tttcacaacc
    14161 tcttggtaca cggtgttcca gaagtagctt cttcggcaat aagccgaaat atcacaaaaa
    14221 tttgagaagc aattttcgta aattccttct tatttcttga agctgaccac ttctgtcaca
    14281 acctctttga ttacttcgtt tgattttatt ttaatataag ttgattaaaa gagagcatga
    14341 tttgttttat tttctcacta atttttccaa cacaactaag aataccataa aagctgattt
    14401 tggaggaggt tcctactaca agtctaaaac gaaatttaag acttttcctc ataaaatact
    14461 ggtttatttt ggtctcatgt aaaactcgct gaaggcctct tcacttataa atgcttcccc
    14521 agctgccagt acgtctttca tttggatcga atcgatgatt tcaggaagat caaataaagt
    14581 tcgatcgcca aataaacttt gcgtaaattg attggcaata tattcgatcg aatttaatga
    14641 ttggaaatat tgtccaagca ttttcttctt cagcaaatcg agatttgttt ctgacacttc
    14701 cggatcatca gcgaatccaa gaatgatttc tttgactttt tctgctgctt tctctggttc
    14761 gtccgtatct ccgctgaagt ccgcaaagtg aaattcacga tccaagctga attcaaaacc
    14821 aaagctgtca tcgataattc cttgattgta catcgctaga tagtttcgag aagtattgcc
    14881 taaaatcatt tggaacaaca gattgattgc tgttttatag cgtaacagtt ctgttccttc
    14941 ttgcggcaat gtgtctaatc ctttaatacc aagtacaaat ttatctcgtg taatggctgc
    15001 ttccaatgca ctttgtttga tgatttcttt tgtgttttca gggaaataac gtacaatttc
    15061 ttgctttgga gggaaatttt ttgcttcttg gttttcccga ataagtttca tcaatttctc
    15121 aggctccatc ttaccaacta caaataaaac catattgctt ggctgataaa acgtccgata
    15181 gcacgtatac aagtcttgag ctgtgatttt gtcgatactt tcaaccgtgc ccgcaatatc
    15241 gatatgcaat gggtgtgtag gatacaagtt atttagtatg ccaaaaaaca tccgccagtt
    15301 agggtcgtct tcatacattt ggatttcctg accgatgatt cctttttcct tgtttacagt
    15361 ttcttctgtg aagtaaggtg cttgtacaaa atcgatcaga gtcgtcagat tcttttctac
    15421 ctgatccgtc gtagaaaaca gatagctggt tttcgtaaaa cttgtgaaag cattggcgga
    15481 agctccttgt ttcccaaata attgaaagac atcaccatct tctttttcaa acaacttatg
    15541 ttctaaaaaa tgggcaattc catctggaac tttcaccttt tctttttcac catagggaat
    15601 aaattcgtta tcgatcgaac cataattcgt actgaacaaa ccatatgtct tgtgatagtc
    15661 atttttaggc aacaaataca cggtcaaacc gtttggcagt acttcatgat aaagtgtttc
    15721 attgatttgc tcatattctg ttttattcat ttgcatttcc tcctgctaaa aagaagattg
    15781 cctgcaattc gatttgctca gctacttgtt gtatctcttt gatcgttacg ctctccatac
    15841 gtttcaacca ttcttcatca gataattttg tttccggcag ccagctgtcc aaaaaagaag
    15901 cttcgattgc cgcttgcgga gagtctagag aaagcagata ttggtttctc agcattgctt
    15961 ttgtctgaga cagttccaac tctgttattt caccatttcg caaactttct aattgctcgt
    16021 gaatcagatg aagtacttga tttcggttct tttcatcgat accggtttgg acactcatga
    16081 atcctcggaa cgtatccacg ctactagagg cataataagc tagactttct ttctctctga
    16141 cattcatgaa aagtttagag tgaggaaaac cgccaaataa accgttaaag accattagcg
    16201 caaaacgttc tggttcatcg taatacacat ttgtctgata agctaggttc agctttgctt
    16261 gaacgattgg ctcacgtact tgtcgttcct tcacgatatt cacttgaggt tgggtataaa
    16321 acagatctgg ataaactgtt tcccgcgact caaatggtaa ttgcccaact gcttcagctg
    16381 ctttgtccgg atcaacatcg ccgacaacaa aaatgtcgat ttgatcattt gccatcattt
    16441 cttgatagta ggacgctagc gaactagcag tgatcttgtc caaatcttcg actacgccca
    16501 accctgggat cttttgttcc gggctgtttt caaaataaag ttcttgcaag gcataggaag
    16561 caaaagtttg tttatcttcc ttcaaacttt ccaaatatag acgcagattg ttcttttcca
    16621 agtcaaacgt caactgatca aactgatgat ttttaatatt tggataaaat aaaacctctt
    16681 ttaaaaaatc taccgcttga gaaaaaaggt ttggatcatt gacatattca ccattcacaa
    16741 aactgatccc tgcgtttagc caatgtaaat ttcctttttt tctaactgat aatccaaagc
    16801 ttgcgccata aagctctgca agttgcgcac ttaactttgt ctgatccgga taattcaaac
    16861 tatttgtttc caacatgctg ctgagaagtg aacgtttcgt gatgacttct tgttgcagac
    16921 gcgcagtaaa acggataaaa atccggatcg ttttgtattg ttttgttggt aagacatgaa
    16981 gattcacgcc ttctgccaat tgatacacca tatttatatg ctccttttca cagttgattg
    17041 cccgcttact cttttaatgg gtcaacctgc cttttttact tattcctctg cttgaaattt
    17101 ataagttcag gcaattcgcc gttttttcta aatgatgtta cactttatag tatagcatag
    17161 atgtttttta tcaaaagaaa cagacggcta aaagcgtata ttgttttttt attttttatt
    17221 tttcgatata ctaaagacaa ataaaagaag ggagggtcat tcatgatcaa agaattcaaa
    17281 gagttcatca tgcgtggaga tgtcttggat cttgctgtcg gcgttgttat tggtagtgcg
    17341 tttacaagca tcgtaaacca agtggtgcaa ggcttgatca caccactaat aggttggttt
    17401 gtcgcaatga tcacaggtac ttctgattta gaaggagcac tttcaattct tgactggtcg
    17461 ccgacaaaag gcgtcacatt tgcgtttggt aacgttatta gtgctatcat tacatttctg
    17521 atcactggtt ttgtcttatt cttagttgtc aaagcggcaa accgtgcgaa gaatttacgc
    17581 tccaaagaag agcaagtgga agtggaagaa actcctactg cagaagatta tcttagtgac
    17641 atacgcgatc ttcttgccaa acaagtggca gaagacaata cacatccagt agagcaaaca
    17701 aatctttccg atacttctaa ataataaaga atctaaaaaa aagaaagccg attttcagtg
    17761 tcggctttct ttttttagat tcttaacttg ctacttgttc ctttttcatg tcgatatgtg
    17821 gaatatcatc ttccaaatac acatctgaga ttgccttgaa tccaaacgac tcataaaaag
    17881 cttgtaggta ttcttgtgcg ccgatcacga ttggtttttc tggaaaattt ttctggatca
    17941 tgttaatcgt ttcttgaaca atctttcttc ctaaaccatc tttacggttg tcctgatgaa
    18001 caaggacacg tccaaaatgg atactctccg cttcttcata tactcgcgtg taggcaagga
    18061 tcttcccatc agtatcttga aaaaacgtat gccaagcttg tccatcaatg tcatcaactt
    18121 cttgataagg acactgttgt tccacaacaa aaactgcgat tcgttctttg acgatcgcgt
    18181 aaaactctgc tgttgacaat tcttcaaatt tctttacctg atattccact aagcaaacgc
    18241 cccctgcttt tttattctta gcataccgat tcgcacacaa aaaagcaaac gaaccttacg
    18301 aatctcaggc tcgtttgctt tttaaactgc ttcagttaaa aattcgatct caactgtccg
    18361 agtaagtaca tctgaatagc tgtaagatac tcgttcaaaa gaattttctt caggatccaa
    18421 atcaacaacg aatactgaag gataggtttc tgttaaaacg ccgcggcgtt ctgtttgacg
    18481 ctttcttcct gtctgagcaa ccaacatgat tgggctgccg atacgacctt ctaaatcttt
    18541 cttgattgaa gctaaagttg ttggcataca gttcacctct actatcaact ataccacata
    18601 tttttaaaat tttcaagttt accataaaaa aaaaagaaat gcaataaaaa taaataagaa
    18661 gctcattaag atttttctaa attattcctc attataaaat ttcaagtgtt tttttatttt
    18721 gagttttact ctcccgtaag cgctcattac ttgcttgtga tccattgcta aacattcagc
    18781 catgtctaca aaatcaacgc cttgcatata ataatgaaaa attttagctt ctatttttga
    18841 aaactggatt tcgcaatttt cgattgcttc ttttaataaa agatgctgaa tcgctaaagg
    18901 ttcttgcaat gtgatctctg ctaaaaagtc tggtccttct gtctctattt tttggtcgat
    18961 tgagacttca tctttttgat cacgacgctt cagcgcgttc tgcttcctca acagagaata
    19021 gatttgattg cttataatgc ttttataata acttccaaag ctaacacctt tgcttttttc
    19081 gtaagttctc aatgcctgat ataaagcgat ccttgcttct tgttcccaat cctcagaaac
    19141 ataatttctt aaataatagg tgtttttcaa acgtagtaaa agtggaaaat atttttgata
    19201 cagggcagca aatgcttctt cgtcaccatt acgtgcagcg cgcaataact cctcagacat
    19261 aaaataattc ctccataaat tcgtttatgg aggaatctta tcatcaatga ttcatcgaac
    19321 tgattagaac gatatgttat ttattttttc tcattaaatc gtccaatttt tgggacaatt
    19381 tttttaactg ctcttcattc catggagaat tacgtcggaa atcaataaaa tgcatatctt
    19441 tattttcttg ggctattgtt ttttccgttt tttttatcgt cttatataat tcataagcag
    19501 aagttcgtaa tgctccttgg gaaaagatca tccactgttc tgctaaatcg cttgtcgcca
    19561 ctgtcacttg tgtcaaacgg tcattcagct gaccagcgat tctctcgata taactgtcag
    19621 ccgtttcgtc ttctttcgta aaaacaacat ccaactggta tttcttataa ttttgttgga
    19681 tgcccggaac tagttgggca tcgaagacaa cgatgacttc cattccttca tatttcgcat
    19741 aatttgataa acgatgtaaa agagcttctc ttgcatccgc cattttgtcc tgctttttaa
    19801 gctggacaag ttccggccac gaaccaatca tgttgtagcc gtctacaatc aacagctgtt
    19861 ttttcatggt ctactccccc tgacgttggg aaaaagcctg atacataagc aaaccagcag
    19921 caacactggc atttaagctc tggacgtgcc cggtcatcgg gatcgtcagc aattcatcca
    19981 tttctttttt caatcctgga ctcattcctt tgccttcatt accaatgact aaagcaattg
    20041 atcctttggc attccatttt tggaagtttg tgccggtcat atctgtgcca aatacccaaa
    20101 atcctgtttc tttgagctgt tgcacagctt gtaccaaatt cgtcacgcgt gcaacaggga
    20161 catgttcgac tgccccagtg gaagtctttg ttacgatagg tgtgattcca actgctcggt
    20221 gttttggtat gatcacacca tcgacgccac ttgcgtctgc tgtccgtaaa atcgacccaa
    20281 aattgtgtgg atcttcgatg ctatccaaaa tcaaaaagaa ggggtcttct tttttctctt
    20341 ttgttcgttc aatcagttgg tcaagggata aatattcata aggtgtaatc gcaagaacga
    20401 ctccttgatg cacgccgtga tcgctcaaag tttctaattt ttgtttagga acccatttga
    20461 ctggcacagc ttgttcacgt gcgatttctt tcagttcttc tactttttct cctcgtgaat
    20521 cttcttgaag aaagagttta ttccctcgcc cttgttgtag cgcttctact gcggcatggt
    20581 gcccaaagac gaaattatct tctaatgctt cttcattttg atcagaaact gactgatttc
    20641 gtccagtttg ctttttattt gaatatctat tttgtttttt gtcttcaaat ccacgtttcg
    20701 attgatgttt gtttgttctt tctttaccca tttttttcac caactttctg aatacaccag
    20761 cggatcaatt cttccatccg ctcggtttgt tttgtcaaat gcagatagcc catgagcgct
    20821 tcgaaccctg ttgcgacccg atacgtcgta acatcagcat tttttgcgct cgtatggctt
    20881 ttagcatttc ttcctctgcg ataataaagt tcttcttctt ctgtcaaaag atgttcttcc
    20941 agcatttgct gaatcaaaaa ggcttgtgca ttcgcagaaa cataattcgt tgccgcacga
    21001 tgcagcttat tgggttttgt ttctccttga tcaatcaggt ggtcacgaac atagatttcg
    21061 taaatcgcat ctccaacata agccaaggct aaaccgttca attgtgtgta atctctcatg
    21121 cttctcttct ccatcgggtt ccttgcggtg tatcttctaa taagatccct ttttctttca
    21181 acaaatcacg aatttcgtcg cttcggacaa aatcacgatt gcttcttgct tgatttcgtt
    21241 cttcgatcaa ttgttcaatc tcttcatcta ataactcttc tgctgtaaat aaaataccga
    21301 agatcgaaag ccattgcgag aataaagctt ctccttgtgc caagacagcg gcagaaactt
    21361 tcggctggtc tagataagtg ttcagccact tagcaagctc atagaccact gtgatcccat
    21421 ttgctgcatt aaaatcatcg tccatttctt cgatgaatcg ctgttctaaa ttttctaaca
    21481 aagcgatttt atccgcatca tctgccaaat tctctgaggc agtttcttgg cggaatttca
    21541 aattgtcaaa cgtattcttc agtcgttgta aattggtggc tgcttctttc atcgtcgttt
    21601 cactgtagcg gatcggacga cggtattgtg tggtcgccat gaagaaacgc aagacttgtg
    21661 gatcaacgtt tttgatcatt tcatgtacag tgatgaaatt ccctagagat ttactcattt
    21721 tttcatcgtc ttcgccaatc gttacatagc cattgtgcat ccagtaatta gcgaattttt
    21781 tccctgtttt cgcttcactt tgtgcgattt cattttcatg atgaggaaat tctaaatctt
    21841 gcccgccacc atggatatcg atcgtttctc ctaaatgttt tgttgccata acggaacatt
    21901 caatatgcca accgggacgt cctttgcccc atggtgaatc ccaagaaatc tcgccaggtt
    21961 ttgcttgttt ccataaagcg aaatctaatg ggtcttcttt ttgctgctgt tcttcccctg
    22021 tacgctgact tgctccgact tccagttcat cgatggattg gtcacttaat ttcccataat
    22081 tttcaaattt acgggtacgg taatacacat cacctgaaga ttcataagca tagccttttt
    22141 caaccaacac ttgaataaat gctaaaatat ctggcatatg atccatgact cttggatgca
    22201 gtgtagcagg ctcgacattc aacgctttcg tatcttcttc aaatgctcgg atgaaacgat
    22261 cagcaacttc tggtgctgta atacctaatt cgtttgctgt acggatgatt ttatcgtcta
    22321 catccgtgaa attcgatacg aaatcgactt tatagcctcg atattcaaaa tagcgtcgaa
    22381 ttgtatcgaa agcaatggtg ctacgagcat ttccgatatg gatataatta tacacagtcg
    22441 gtccgcagac atacataccg acatgtccct tttgtatagg gatgaattct tctttttttc
    22501 tggtcaatgt attgtagatt tttatcattc tgatctcctc actttttctc ctttgattcg
    22561 aacgactttt gctggtactc ctaccgctgt agcgtcgtct ggaacatctt tcaacacaac
    22621 ggcacctgca ccgattttgg cgttttttcc gatcgttacc ggtcctaata tctgcgcatt
    22681 cgcagagagc atggctcctt gtttaacagt tgggtgtctt ttacccgttt cttttcctgt
    22741 gccgcctaat gttacgccat ggaataaaat cacatcgtcc tcgatttctg cggtttcgcc
    22801 aatgacgatt cccattccat ggtcgatgaa cacgccagtt ccaatcgttg ctcctggatg
    22861 gatctcaaca ccagttagga aacgccagaa ttgagcattg atcttcgcca ataaaaagag
    22921 ccggtgctga tataaaaaat gagagaaacg atgccaaaac aaggcatgaa aaccagggta
    22981 tgtcaaaacg atctcagctg tcgaacgtgc agccggatcg tttctctttg ctgcatcaat
    23041 cgctcgggta aaccaactca tgactttcca ctcctttcgt aagaatttta tgtaggaaat
    23101 catcttctta taaagaaaag aagcgccttt taaataagtt tccttatcaa aagacgcttt
    23161 ccgcgtggtt ccacttttct tcataatcaa tgattatctc tgacagctaa cgcctgttat
    23221 acgttcttgg ctactttgtt tcctataaaa cagtttttcc tgttttctgt gtttcgccaa
    23281 gaccactccc agaggcattt cggcaaccat tcattgatac cttgcaccac ccggtatctc
    23341 tcttgtaatg atcagctgct tacttttttc tgttcatcgt gtttagttca gtacttgatt
    23401 taaatgtgct aatgattttt cacgaccaag caattcgatc gtttcaggca attctggacc
    23461 atgcatttgt cctgatacag ctacacggat tggcatgaaa agattttttc cttttacgcc
    23521 agtttctttt tgaacagctt tgatggcagc tttgacagtt ggagcatcta ctacttccat
    23581 ttgttctagc tgtgctttga atgcgtggag aacagtagga actgtttcac cagcaagtac
    23641 ttcttttgct gcttcattca atacaggatg ttcattgaaa aatagcgtag acagatcaac
    23701 gatttctgca gcatagctca tttgaggttg atacaggcta acgatcttct tcacccattc
    23761 gattgtcttt tcatctgggt tttctgctac acgtccatca gcgattagat aaggcagaca
    23821 catgtcagta aatgcatcta gatccattgc tttgatgtaa tggttgttta cccattgcag
    23881 ttttttcgca tcgaaagctg cgggtgattt acttaggcgt tttggatcga acattttgat
    23941 caattcttgg tgttcgaaga tttcgtcttc gcccacaggt gaccagccca ataatgcaat
    24001 aaagttgaac attgcatctg gtagatagcc taattcgcga tattgttcga taaattgcaa
    24061 gattgattcg tcacgtttgc ttagtttttt acctgtttca ctgttgatga tcaatgtcat
    24121 atgtccgaag gttggtggtg tccagccaaa tgcttcatag atcatcagtt gttttggtgt
    24181 attggcaata tggtcatctc cacgtaaaac gtgagtgatt ttcatcaaat gatcatcgac
    24241 cgcaacagca aaattataag ttggcatacc atcgcgtttt tgtatgacaa agtcgccacc
    24301 gatattatcg gattcgaacg aaatagcgcc tttcaccata tctgtaaatg cgtattctgt
    24361 attgcgaggc acacggaaac gtacaacaga ttctagacct tgtgcttctt tttctgcttg
    24421 ttcttcaggt gtcaagttcg cacatgttcc tgcgtaatga ggcatttctc ctcttgcgcg
    24481 ttgtgcttcc cgttctgctt ctaattcttc ttctgtgcaa tagcatttgt atgctcgatt
    24541 gctagcaagc agctgatcaa tcagcggttg atagatttct ttacgttcag attgacggta
    24601 agggccgtat tctccaggat tttcaggaga ttcatcccaa ttcattccca accaagcaag
    24661 gttttccaac tggctttttt cgccatcttc gatattacgt ttttggtcag tatcctcaat
    24721 acgaataata aagtctccat cattgtggcg ggcaaacaaa taattgaata atgcagttct
    24781 tgcatttcca atatgcaggt gcccagttgg acttggtgca tagcgtacgc gtactttcgt
    24841 cattttcttc ctcttctttc aaactagact ttgactaaca gcaaaaattc cgtcagttac
    24901 ggcttctcaa atatcattta tttaaagcca ctcagcacaa attttacctt ttctaaggta
    24961 aatagtaaag ccaactggtt gaatttcctt accagttggc tgaatttctt ttttattttg
    25021 attgtttagt ctttttctct gtctcttttt tattgtcgat tcctcgtcct gcatgtgcag
    25081 gtttagcaaa gatcatgcgt ccagcagctg tttgaagggc acttgttaca acgacttcga
    25141 tacgttcatt catgtaatgc tgtccatctt ctacgacaac catcgtgccg tcatcaaggt
    25201 aggctactcc ttgttggcgt tctgtcccgg cttttactac tagaacattc atcgtttcac
    25261 cagggatgac gactggtttt acggcatttg ccaacgcatt gatattaaga acaggtacat
    25321 tttggaattc agagacttta ttcaagttat agtcattggt tacaatgaca ccatctaaaa
    25381 gcttagccaa tttgatcaat ttgctgtcta cttcactgat atcttcaaag tctccatcgt
    25441 acatttcgac agatataccc tcttcttttt gcaaggcatt taggatatct aatccgcgac
    25501 gaccacgcac acgtttcaaa ctatcccctg agtccgcgat gtattgtagt tcgtaaagaa
    25561 caaaattagg gatcatcaag gttccttcta agaatccagt ctttgtaata tcataaatcc
    25621 gaccgtcaat gatcacgctt gtatccaaga ttttatattt atggaaattt tctcctgatt
    25681 ttcgttctaa cagttgactc tcaacttgac tagctgtttc ttgctcgatt tttttgcttc
    25741 gtgaaccaaa aactttcttc cattcttcga ttcgtgtcgt tcccattctc aatcccagat
    25801 agcctaagat gatcatcagt aaaatcggca atacactatt gacgaatgga atttccatat
    25861 tgtaaagagg ggtcgagatc acaacaccta aagtcaatcc gatgatcgca ccgatcgcac
    25921 cgaagagaag ataagtcaag ctgacctcac ttagtttttg ttctacttgt ttgataatag
    25981 ctgagatatg tttggccaac agcaaagata ataaaaagaa aataagtgcg cctaacaggc
    26041 tgttggtaaa tgtattattc agccatccgt ttgactgttg accgattgcc tgccaagctg
    26101 agggtaaaaa tgaaataccc aagcttgcgc cgacaatgat catcagcaaa gtaatgacac
    26161 gtttttgcat cgtttttcct ccgtttcgac aatcatttat tagttagcgg aacacacgct
    26221 tcaacgtttc agccaaagtt gctactccaa ctatctcgat tccttttggc gcttcccagt
    26281 tacctaagtt gtttttaggt aggtatactt ttgtgaagcc gagtttttgg acttctttga
    26341 ctcgttgttc aatatgactg acacgtctga tttctccagt cagtccaatt tcaccgatga
    26401 aacattccgt tggctgtgtt cctttttctt tatagcttga agcaatactg acagcgatag
    26461 ctaaatcaat cgctggttca tttaatttta ccccacctgc agcttttaag taagcatctt
    26521 gattctgaag aagcaaccca gctctttttt ccagcactgc catgatcaga gatacccgat
    26581 tgaaatcaag acctgttgtt gttcgtttgg catttccgaa catagtaggt gtgactaatg
    26641 cttggatctc taccaaaatt ggacgtgtcc cttccattgc cacaacgata gcagagccag
    26701 ttgcaccgtc taggcgttct tctaagaaaa tctgtgaagg attagcgact tcttccaatc
    26761 catgctcatg catttcaaaa atcccaatct cattcgttga gccaaatcgg ttttttactg
    26821 ctcgcaaaat ccgaaaacta tgatgttgtt caccttcaaa atataacacc gtatccacca
    26881 tatgttctaa cattcttggg ccggcgatcg agccttcttt ggtaacatga ccgacgataa
    26941 aaatggctat cccatttgtt ttagcgattt tgagtagttc tgcagtggtt tctcttactt
    27001 ggctgacact tcctgccaca cttgtgatgt ctggctgcgt catcgtttgg atggaatcaa
    27061 tgataacata atcaggggag atatgttcga tggcacgact gatttcgttc atatctgttt
    27121 cagcgtatag ataaaattct gtgttgatac ttccgaggcg ttccgcgcgt aattttatct
    27181 gctctgcgct ttcttcgccg gatacatata agacttttcc gcctatggct gctaattgct
    27241 gagaaacttg taacagcaaa gtggatttcc cgatacctgg atcgccacca atcaagacca
    27301 tagaccctgg caccacacct ccgcctaaca cacggttcag ttcttctaat tttgttttga
    27361 tccgaggttc ttttttaggg acgacgtctg cgatttttgt tggttttgct ttttctcctg
    27421 tcaaacttgt ccgagttctg cggtccgttg tatcttgttc gatttcttcg accatcgtat
    27481 tccattttcc gcaatttggg cagcgtccca agaacttagg cgacacatac ccgcaatttt
    27541 ggcaaacaaa ttgtactttt gatttttttg ccattgtacc gcctcctttt atcctaatca
    27601 tgagacatta aatcaagctt aagtatcttt aacgttttat ttactggaag aaccaaatcc
    27661 gccttgacgt tgagcagtcg tttgatcttg atctgctttt aaaaacggca aaaagatccc
    27721 ttggccgata cgctcgcctt ttttgatcac tacatccttg aaaccaaagt tggtgaattg
    27781 gaacatgata tggccttcat ttgcctcatt attgtaataa tcactgtcaa tcacacctac
    27841 tccgtttgtc aacactaaaa accgcttcaa cggattgctt gaacggttag caagctgcaa
    27901 aaattcatct tctcccatgt aagctttgat tccagtaggc acgagggttg gtttcaattg
    27961 tttttcattt tctgatacat cttttttcaa tccatctgcg actgtttcca accccttttt
    28021 ccagcctgct ttccagatac ttggaatcac gacctcttct gctgcttcaa aatcatagcc
    28081 agcagcatga tgtgtagcgc gctgcggtag attgatattt tgatctttat atttagaaat
    28141 aacttcaaat cctctttttc tcaccggttt tcct
//
LOCUS       DACGLW010000030        27083 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.030, whole genome shotgun sequence.
ACCESSION   DACGLW010000030 DACGLW010000000
VERSION     DACGLW010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27083
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.030"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            7..624
                     /locus_tag="IXT53_001334"
     CDS             7..624
                     /locus_tag="IXT53_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905174.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTIVEVSSTYPTTMILSLLISSITFIFLFTRYLIRCCGEMKID"
     gene            1644..2813
                     /locus_tag="IXT53_001335"
     CDS             1644..2813
                     /locus_tag="IXT53_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ4905175.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
     gene            3286..3840
                     /locus_tag="IXT53_001336"
     CDS             3286..3840
                     /locus_tag="IXT53_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ4905176.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(4013..4486)
                     /locus_tag="IXT53_001337"
     CDS             complement(4013..4486)
                     /locus_tag="IXT53_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ4905177.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            4498..5034
                     /locus_tag="IXT53_001338"
     CDS             4498..5034
                     /locus_tag="IXT53_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ4905178.1"
                     /translation="MPARIHHISIVNRFIRPTFDFYHNILGLKLLMKTINQDDHTMYH
                     LFFSDNHHRVGTELTFFEVQEGNNQFFGTNTIERTILKVPSEASLHFWEIYFETQGVC
                     HYGIESFSGRPILRFEGPDATRLALVPLREFEDIDDYFPYVTDQIPTEHAILGIDAIQ
                     LRVQYSDATERELLSVGW"
     gene            5074..5445
                     /locus_tag="IXT53_001339"
     CDS             5074..5445
                     /locus_tag="IXT53_001339"
                     /inference="COORDINATES: protein motif:HMM:NF013098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ4905179.1"
                     /translation="MTILENQDTQFYQEVQIIQDRENPVAIEGIGSVHHVAFGVENKS
                     DLQRIDKQLQERNFINSGIKDREFFVSLYYRDPNQLLIEIATGEGNLDAKAYENQSPR
                     FEEIPLFLPQYLNFIREQVEQ"
     gene            5729..7000
                     /locus_tag="IXT53_001340"
     CDS             5729..7000
                     /locus_tag="IXT53_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HAQ4905180.1"
                     /translation="MKLSTQEKQLKNVSLKIPKNKISIFTSVSGSGKSSIVFDTITQE
                     AGRQLNDTYDSFTRLFLPKYSRPEVDEIDNLSTAIVVNQKKIGGSARSTLGTITNINA
                     LFRVLFSCFATPSLGYANAYSFNDPSGMCSVCQGIGKTIAFLIGLLYFSAIDNADFVF
                     SDPFTAEVLSGIEEQAKNHGYFVLVHNVNSAEDVKTIQRNWRFEGFIVVGVRVKEFLE
                     LNNVFDGPVVYIDTHLSKETLRELEQQMNRLFVNTNDYQASTLAVEHLIHYGHEKIAF
                     LSFDYIVGEPSVIQERHQAYIDTLKKYHLTFKSHWIYNNSEFERIYQELAEFTAIVVT
                     GDYLAAKLVKFLKNKNKRVMEQLSIIGFDDITFAELMDPALTTIRLNPGNKGAIAFHQ
                     LIGLAKQEEIKNQVLLLEGELISRDSVHKIK"
     gene            7245..8270
                     /locus_tag="IXT53_001341"
     CDS             7245..8270
                     /locus_tag="IXT53_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ4905181.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            8564..9673
                     /locus_tag="IXT53_001342"
     CDS             8564..9673
                     /locus_tag="IXT53_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ4905182.1"
                     /translation="MLDEFEKQNPDIKVEMETTPWDQYWVKLEAATTGGNMPDVVTMH
                     SSESYKYMSQNALMGLDDVIKENDIDMGNFTEGIADFYTLEDELYAIPKDASVVGLWY
                     NKELFDNAGVDYPDETWTWETLRQAAIDLTDESKGIYGFASHIGTETGYWPFIYQNGG
                     EVFADDNTKSGLNTPEAQEALAFYRDIALVDHASPTITELQDPDKLSRFQSGRLAMMI
                     EGNWFAPGFLKNDYLVENVASAVLPKGKVNATITNGLGWAASASTKYPEEVKKLMAFL
                     ASEDANKIQAETGASIPALKGFEDEWAASSDTIDLSAFGKMLEYGVPRPYNKDGLRAE
                     QAEVQIINRILSGEVTVEDGTAQIEKEVNAILSGN"
     gene            9756..10652
                     /locus_tag="IXT53_001343"
                     /pseudo
     CDS             9756..10652
                     /locus_tag="IXT53_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008378728.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            10668..11492
                     /locus_tag="IXT53_001344"
     CDS             10668..11492
                     /locus_tag="IXT53_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002579255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ4905183.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            11524..12834
                     /locus_tag="IXT53_001345"
     CDS             11524..12834
                     /locus_tag="IXT53_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HAQ4905184.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            12982..14241
                     /locus_tag="IXT53_001346"
     CDS             12982..14241
                     /locus_tag="IXT53_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ4905185.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            14251..15123
                     /locus_tag="IXT53_001347"
     CDS             14251..15123
                     /locus_tag="IXT53_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ4905186.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            15135..15965
                     /locus_tag="IXT53_001348"
     CDS             15135..15965
                     /locus_tag="IXT53_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ4905187.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            16005..18188
                     /locus_tag="IXT53_001349"
     CDS             16005..18188
                     /locus_tag="IXT53_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAQ4905188.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDKTVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            18249..19469
                     /locus_tag="IXT53_001350"
     CDS             18249..19469
                     /locus_tag="IXT53_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-1,6-glucosidase"
                     /protein_id="HAQ4905189.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            19466..20926
                     /gene="gtfA"
                     /locus_tag="IXT53_001351"
     CDS             19466..20926
                     /gene="gtfA"
                     /locus_tag="IXT53_001351"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HAQ4905190.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            21003..22109
                     /locus_tag="IXT53_001352"
                     /pseudo
     CDS             21003..22109
                     /locus_tag="IXT53_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            22431..22640
                     /locus_tag="IXT53_001353"
     CDS             22431..22640
                     /locus_tag="IXT53_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAQ4905191.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            22633..22782
                     /locus_tag="IXT53_001354"
     CDS             22633..22782
                     /locus_tag="IXT53_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HAQ4905192.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            22817..23601
                     /locus_tag="IXT53_001355"
                     /pseudo
     CDS             22817..23601
                     /locus_tag="IXT53_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922412.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(23885..24970)
                     /gene="xerS"
                     /locus_tag="IXT53_001356"
     CDS             complement(23885..24970)
                     /gene="xerS"
                     /locus_tag="IXT53_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HAQ4905193.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            25304..25537
                     /locus_tag="IXT53_001357"
     CDS             25304..25537
                     /locus_tag="IXT53_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HAQ4905194.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            25534..25941
                     /locus_tag="IXT53_001358"
     CDS             25534..25941
                     /locus_tag="IXT53_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HAQ4905195.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
     gene            complement(26392..26901)
                     /locus_tag="IXT53_001359"
     CDS             complement(26392..26901)
                     /locus_tag="IXT53_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905196.1"
                     /translation="MKKVLFAVSLVVNIALAGGLFYTITEMNKLDDQRESLAISNEQY
                     KRKLDEASSTNVDTQEAVESSTIVYSNESSETLTNSERSTAPATETVSQKATITIGSK
                     VFTFIVNGLTIEHDSSRTGKMTELSHEFPVIVTTPSATLNGILVQDNNDNNCYIMVDG
                     QTLYRGTYQ"
BASE COUNT         9319 a         4024 c         5036 g         8704 t
ORIGIN      
        1 agaaccttgg gttttcaagt gctttttggt tcattaatct taaccatagt gtttttaaag
       61 tggaaaacaa gcaacaaaaa aagtatgtgg agccttgtag gaatagttct tattattttt
      121 actgtgactg cagcaacaga agcaaattat tcgcataaaa ttcagtcgta taaaggtcgt
      181 ttagttatga ccaaaaaaga tgttacacgt tatacatcct caagaactgg aggcctatat
      241 gactatgcac cattagatgc attgaaatat aaagatcatt taaagaaaca tgaagtaaaa
      301 gttggtgatc ggtgggagaa tgcatctcat aaagcatata attccaaaat agtctataca
      361 gtagaagatg ccgatgagca aaatatcaca ttacctgtat tcaattattg gggaacagtt
      421 gtgaaagtaa atgggaaaaa gagcactata gaaaatgaca atggtttagt gtctattaaa
      481 ggtacgggga aaagaactat agttgaggtt tcatctacgt atccaactac gatgattcta
      541 tctttattaa ttagttcaat aacttttata ttcttattta cacgttattt aataagatgc
      601 tgcggtgaaa tgaaaataga ttagatagat attataaata tgttgattct attttcatag
      661 caatggagaa aatacattag caatatataa aagaaggtat caagagtcac agtgaattta
      721 ttccgaaaag tgaaaagagc aaatataaaa tttaatgaaa atatagaact aaataggaaa
      781 acgtagacgt aattttctac gttttccttt tcatatatat taaatattac ggaaaattat
      841 ataaactaat ttagtgtttt gtttgtaatt cctaattttg ctccactttt agataatttt
      901 taggtctatt taactcatca gacattctat gctcattttt ctccaaattt gtttcaactc
      961 tttcgtactt aattttgcgt atttgaaaat tcttctttta gagtactttt attacgatac
     1021 tagtatgata tatatctata gcaaacagtt aaaatcttcc taaaataagc gaatagcatt
     1081 cctaaaacct ataaaatttt tagatgaagc gacaagtgga caattaataa actacttgaa
     1141 catgaaatta tggatcacct ttcaaattta gaaaatggaa ttcttatact tattgcgcaa
     1201 catttattga ttgcaaaatt atgtgacaaa aaaaactagt agaagaaaga ataatggctt
     1261 gataataaag tactacctat taaaggtagt actttattta atttagtata aattaacata
     1321 ctactatatt ttaggtttca tattatataa attaataata tactaccatt tgttggtagt
     1381 ataatttaaa ggtaataagt tattctttta ttactatgtt tgggttttat agcaaagaaa
     1441 gtttaataag agtttggacc tagcaaacaa tattaatgag gtcattaaat cttattatct
     1501 ttagtgatat aacgaaatat ctacacagac aaaatgatag cgtaaaatat tttgtgtaaa
     1561 tagatattta gggtagaaaa aaagaaagtc cattctgtaa aattaaagtt accacaaccg
     1621 aaattttata ggaggacttt ctcatgccca attttactac agaaattatg gaaacactaa
     1681 tcaataaagg tgatttagat gaattatttc gatgtcattt agaattagct gttaattcat
     1741 tattgcaggc tgaattaaca gcttttcttg attatgaaaa atatgatcgt gctgggttca
     1801 attcaggtaa ttcccgtaat gggaattatt cacgttcatt cagaacagag tatggagaat
     1861 taaatttggt aattcctaga gatagaaatg gtaagttttc tcaacaaact ttacctgctt
     1921 ataaaagaac caatgactct ttagaaacca ctattattca gctatttcaa aaaggaatta
     1981 ctatgtctga aatctctgaa ttgattgaaa aaatgtacgg gcatcactat acgccacaaa
     2041 ccatttcaaa tatgactaaa atagtttctg aagatattat tgcctttaaa gaaaggtcct
     2101 tagaatccag atactctgtc atttttatgg atgctactca cattccttta aaaagacaaa
     2161 ctgtttcaaa agaagcagtt tatattgtaa ttggcattcg attagatgga accaaagagg
     2221 ttcttgggtt tagtcttgct ccaactgaat ctgcttatgt ttggaaagag attcttcaag
     2281 acctaaaaga ccgtggttta aaagaggttt tattagtcgt aactgatggt ttaagtggca
     2341 ttaacgatag tattcatagt gtttatccaa atgctcaatt tcaacaatgt tgtgtccata
     2401 tatctagaaa tatcgctcat aaggttcgtg ttagtgatcg acaagaaatc tgtagcgatt
     2461 tcaaattagt ttatcaagca agttcaaaag aagaagcaaa taatcaaata aggtttatga
     2521 tagataaatg gaaaaagcag tatccacgag tggttaaatt gctcatgaat cctgctatat
     2581 taacctttta taatttccct ccgtcaatca gaagaactat ttattcaact aatttgattg
     2641 aaggttttaa caaacaatta aagaaatata ccaaaagaaa agagcaattt cctaatgaag
     2701 aatctctgga gagatttcta gtatctcaat tcaacaacta taatcaaaag tttctatgcc
     2761 gagttcataa aggttttaaa gaaatacatg atacattaga atcaatgttt taacttgatc
     2821 aacagaatgg gcttttccat ttacacaaaa ttattgacgc tcccgacaaa attatgatct
     2881 tgcgtaaagt tataaaatat taataaaaat attttattaa tgaaaagctt ggtagcacgg
     2941 ttattcatat gctaatttat ttcactttat agtaagggga gttcttagta tattgttcaa
     3001 acttaaattt catataattt aattatgtaa attagctatt ctaagaactt ctacggataa
     3061 ctgcccgtct aaaatttaaa taagagctta gaatcttcat ataagtaagg ttaatttttt
     3121 tgtacaaaaa agtcggaata ttctgagtta ctgaaatgat ataaaatcat aaactaaatc
     3181 ccttgatttt attgactttc aaaagtttat atatataagt taatgattat ggtaaaatat
     3241 atacataatc attaactcag aatattttta agaagggaaa caatagtgag ctataatgta
     3301 caaccgttaa ggacaaatga agagatacaa gattttcagt tttggctaag aagaacaagt
     3361 aatccggaaa gagatagttt tttatttttg tttggaatca ataatgggtt acgtatgtca
     3421 gatattatag gattaaaagt tggagatata cgaggcaaaa gtaagcctat tattgttgaa
     3481 cataaaacag gtaagcgaaa accgattttt atagataatt tgagagaaga aattttatta
     3541 tatactgaag gtaaagaaga aaatgattgg ttatttccga gtcggcaaca aggtcgacat
     3601 ataacaaggg atcgtgtcta tcagatatat gcagatatag cagaaaaatt aggacgagat
     3661 gatataggaa cacatacatt aagaaaaact tttgggtatc actactacaa gaaaactaga
     3721 gacattgcta ccttaatgtt tatttttaat catagctcgc aagcaattac aaaaaggtac
     3781 attggtataa ctgaagatga aataggagca tcacttcgtg gatttaaact aggtgtataa
     3841 tttaaaaatt tttataaaca gtaaagacaa agattaaaag ctatttttat ctagaacgat
     3901 tttatttcat aaagttcgcg gaataaaagc tggggttact ggtacttggt ataggttcgt
     3961 gttatataaa ctaagtaatt aaataacatt tttaggttgg aaaaggatga aattaaaaaa
     4021 agagaatcaa tacggtctca aaaacgcaaa cgctgcggaa taagaaaaat actgataact
     4081 caacgtttag cagactatct aatagggata gtctgctttt ttcgtttttg accatacttt
     4141 tgaccttctg caaacttcct tgcttttcct ctgtagaact catgaaatct gcaaaacgaa
     4201 ttgccgtttc ctctgtctgc tttttcgcga tatgggtgta tatatcatcc atagtggttt
     4261 taatatctgt atgtccaagt cgaacataca cttctttgac agacgcacca gattcaaaga
     4321 gcagactaca atgggtatga cgaaaaccat gaagtgtgat atgttttaag ttattctctt
     4381 taatgatgat tttcaaattg tgatttacat agtctaaata aaggggctta ttttcttctg
     4441 atgaaaacaa aagttgttca ccatctttta taggtttgat tctcattttt agatattatg
     4501 cccgcacgta ttcatcacat ttctattgtg aatcgcttta ttcgaccgac atttgacttt
     4561 tatcataata tactcggttt aaaattacta atgaaaacaa ttaatcaaga cgatcatacc
     4621 atgtatcacc tgtttttttc agataatcac caccgtgtgg gaactgaact gacttttttt
     4681 gaagtacaag aagggaacaa ccagtttttt ggaaccaata cgattgaacg aacgatacta
     4741 aaagttcctt ccgaagcatc attgcatttt tgggaaatat attttgaaac acaaggcgtt
     4801 tgccattatg gaatcgaatc attttctgga cgacctatct tacgttttga agggccagat
     4861 gcaacacgat tagctttagt tcccttacgc gaatttgaag atattgatga ttatttcccg
     4921 tatgtcacag accagatccc tacagagcat gccattttag gaattgatgc cattcaacta
     4981 cgcgtacaat attccgatgc gactgaacga gagttattgt cggtaggctg gtagcacaaa
     5041 gaatacgtca gtttcttttc gggtcatcaa cgagtgacaa tcttagaaaa tcaagacact
     5101 cagttctatc aagaagttca gattattcaa gatcgtgaga atccagttgc cattgaaggg
     5161 attggtagcg tccatcatgt tgcgtttggc gtagaaaata aatctgattt acaacgcatc
     5221 gacaaacaat tacaagagcg caattttata aattcaggaa taaaagatcg tgaatttttt
     5281 gtatcacttt attatcgtga cccaaatcaa ttattgattg aaattgctac aggggaagga
     5341 aacctagatg caaaagcgta tgaaaatcaa agtccacgct ttgaagaaat tcctttattt
     5401 ttaccgcagt acttaaattt cattcgtgaa caagtggagc aataattagc taatcaaatc
     5461 catgacgaag agtaagaggt tgactcataa gtcaaaaaat aaagaaaaac gcatcaaatc
     5521 cattctaaag aatgttgatt tgatgcggtt cctattttta taaactatca ttttcgacta
     5581 ataaaactac ttatgagtca gcctctcttt ttttcttgat ttttatacct ctttctaggt
     5641 aaactaaaca tctgccaaaa ataccgtgtc tcattgaaac attctcaact atttcaaaat
     5701 aaaggggagt accaaatgaa cgagtatatt gaaattatcc acgcaagaga aacaacttaa
     5761 aaatgtcagt ctcaaaattc ccaaaaataa aattagtatt tttaccagcg tatctggttc
     5821 aggaaaatca tcgattgttt ttgatactat tacccaagaa gctggtcgtc aactgaatga
     5881 cacctatgat agttttaccc gtttattttt acctaaatac agtcgtccag aggttgatga
     5941 gattgataat ctttctacgg cgattgtggt caatcaaaag aagattggtg gcagtgcgcg
     6001 ctcaacgtta gggacgatta ctaacatcaa tgcgttattt cgtgtgttgt tttcatgttt
     6061 tgcaacacct agtttaggat atgcaaatgc ttattcattt aatgatccta gtggaatgtg
     6121 ttctgtctgt cagggaatcg gtaagacgat tgcattttta ataggattgc tttatttttc
     6181 ggctattgat aatgctgatt ttgtattttc tgatccattt accgcagaag ttttgtctgg
     6241 aattgaggaa caggcaaaga atcatggtta ttttgtattg gtgcataatg ttaattcagc
     6301 agaagatgtt aaaactattc agagaaattg gcgttttgag gggttcattg ttgttggtgt
     6361 acgtgtaaaa gaatttttgg aattaaataa cgtttttgat ggtcctgttg tttatattga
     6421 tacgcatctt tcaaaagaga cattaagaga acttgaacaa caaatgaatc gtttatttgt
     6481 aaatacaaat gattaccaag ctagtacatt ggcagtggaa catttgattc attatggtca
     6541 tgagaaaata gcgtttcttt cttttgacta cattgtaggt gaaccaagtg ttattcaaga
     6601 acgacatcaa gcttatatcg atacattaaa gaaatatcat ttaaccttta aatcgcattg
     6661 gatatataac aactctgaat ttgagcgaat ttatcaagaa ttagctgaat ttactgcaat
     6721 tgttgttact ggagattatt tagcggctaa attagtaaaa tttttaaaaa ataagaataa
     6781 acgagtgatg gaacaattat cgattattgg atttgatgat attacttttg cagaattaat
     6841 ggatcctgcg ttgacaacaa tacgactaaa tccaggtaac aagggggcga ttgcttttca
     6901 ccaactgatt ggactggcca aacaagaaga aataaaaaat caagtactac tattagaggg
     6961 agaattgatt tctcgagact ctgttcacaa aatcaaataa aaaagctctg tgaaataagt
     7021 atagttaaca ataaaatatc cgaacgatag agagaatgaa ctgtgatcta gcagttcctc
     7081 gtcgcaaatt cactagaatt agcattattt actctaaatt gttcggatat tttttcttgt
     7141 ttttaattat gattacagtg cctattttta acgaaatttg cttattttca aaatgtatga
     7201 ttatatgtta gtaaacactt tcaaatatga aaagaggagc tattatggta ggaattaggg
     7261 atgttgcaaa acgggcgggt gtgtccattg caacagtatc gcgcgtgcta aatgaggatc
     7321 cgacactttc agtaaccgat gaaaccaaac gaaagattgc agaatcggtt aatttttatc
     7381 attatgtcaa aaaaagtccc gttgcaaaaa atttaacaaa tattgcatta ataacgacag
     7441 tttctgaagt agatgagttg gaagatccct attttcgaac gattcgcaga gggattcaag
     7501 tcgaagcaga acaaagcaaa gtcaatttga agaaaattat tcgtttgtca gaaacaccgt
     7561 tggagcaaga cgatttgcta aaatgtcaag gtgtgttgat tattgggcag gtgctatctt
     7621 ctgtgattga agaagtatca gcaattaatt cgaatgtcgt agtgattgat gaccctagta
     7681 ttgaagctga agtagatgca gtttatactg atttaacaaa agcaacgaat cagcatttag
     7741 atcgattgta taaaaaagga caccgaaaca ttgcgtttat cggaggagga cgtgttcgtt
     7801 tagatagcca aggaaataaa cacgtgtcag atgatgatta tcgaaaaata gcctatgaga
     7861 cgtggatgaa acaaaaagga ttagaaaaat atagtcaaat ttatttagaa ggctggacca
     7921 cacttgacgg aatgcaggca gcagatttac ttttgaaaaa acatgctaaa gataagttgc
     7981 cgactgctat catggtagga aatgatccta ttgctgtggg ggtttatcgt tcacttcaaa
     8041 aaaatggtct acatattcca gaagatattt cgattgtgag ttttgataat attgaagttg
     8101 ctgaattttt aacacctgca ttaaatacgg tgaatgtgaa tacagaagaa attggaaaac
     8161 tagcagttcg catggttaat gaacgaataa aagaagttag aaatgtttcg atcaaagtga
     8221 ttgtttctaa taatattatt gttagagaaa gtgaaaaaaa tatcgattaa aaaagtaaac
     8281 atccaaaaaa gggtgtttac ttttttgttt actaatattt ttagtaaaaa atctcgacaa
     8341 aacattaagt aaacagttat ggtgttgtta acaaaaagaa aacgcattca aaacaggagg
     8401 aattaagatg aaacatttca aaaaaatagc tttaacaggg atagctgttt taactgggac
     8461 attactcgca gcatgtggtg ggggagatag taattctaaa gacgggcaag taactttaag
     8521 ctatgcgagt tgagatactg accaagctcc aggactaaga aaaatgttag atgaatttga
     8581 aaaacaaaat cctgatatca aagtagaaat ggagacaacg ccttgggatc aatattgggt
     8641 taaattagaa gctgcaacaa cgggaggtaa tatgccagat gtagtaacca tgcactctag
     8701 cgaatcctat aaatatatgt cacagaatgc tttgatggga cttgatgatg ttatcaaaga
     8761 aaatgatatt gacatgggaa actttactga aggaattgct gatttctata cgcttgaaga
     8821 cgaactatac gcaatcccta aagatgcttc tgtagtgggg ctatggtaca acaaagaatt
     8881 atttgataat gcaggtgtcg attatccaga tgaaacatgg acatgggaaa cactcagaca
     8941 agctgcaatt gatttaacag atgaatcaaa aggcatctat ggttttgcat cacatattgg
     9001 aacagagact ggctactggc catttattta tcaaaatggg ggagaagtat ttgccgatga
     9061 caatacaaaa tcaggtttga ataccccaga agcacaagaa gctttagctt tttatcgaga
     9121 tattgcactt gttgatcatg cttcaccaac aattacagaa ttacaagatc ctgataagtt
     9181 aagtcgtttt caatcaggta gattagcaat gatgattgaa ggaaactggt ttgcaccggg
     9241 atttttgaaa aatgactatc tagttgaaaa tgttgcttct gccgtgttac ctaagggaaa
     9301 agttaatgca acaattacaa atggacttgg ttgggctgcc agtgcgagta caaaataccc
     9361 agaagaagtg aaaaaactaa tggcattttt agctagtgag gacgccaata aaatccaagc
     9421 ggaaacgggg gcatcaatcc cagcactaaa aggctttgaa gacgagtggg ctgcttcttc
     9481 agatacaatt gatttgagtg cttttggaaa aatgttggag tatggagtcc ctcgtccata
     9541 caataaagat ggattacgag ctgaacaagc agaagttcaa attattaacc gtattctgtc
     9601 tggtgaagta actgttgaag atggaaccgc tcaaattgaa aaagaagtca atgcgatatt
     9661 gagcggaaat taaattagtt aattatacaa tatacaggcg aaaggattct atttcgtctg
     9721 tatattaatg gatgaatacc aaaaaggggg atattatgaa aaatcgaatt tttggaaaat
     9781 atcgtaaaga tcaagtgata tggggctata tttttatttt tccaacggca ttaggattaa
     9841 ttttattaaa tatatggcca gcaattcaaa cagcaatttt aagttttcaa aaaacggtag
     9901 ggttcggtga tactgaatgg gtcggattaa gaaattacca aaaaatgttt gcagacggtg
     9961 aagtttttca gtctttatta aacacattaa tttatgcagt ggtttcgata cctagtattg
    10021 ttgtattatc cttgttggtg gcagttttaa tgaatagaaa agttaaagga ttgtcgattt
    10081 atcggacaat ttattttcta ccagtcgttg ctgcaccatc cgcagtcgca atgatttggc
    10141 gctggatgtt caataatgat tatggactga ttaataacat gttggctaaa ataggtctcg
    10201 atggaccagc atggttaacg gatccatcga ttgcgattta ttctattatc attgtaggag
    10261 tttggagtgc tattggttat aacatggtgt tattactagc cggcttataa gaaattccta
    10321 aagattatta tgaagcagcg gaaattgatg gtgctagtcc aatccagcag tttttcaaca
    10381 ttacgttacc attagttaca ccaacattat tttttgtagt tgtaacaaca gttatcaatg
    10441 ctttccaagt atttgatgtt atttttatga tgattactcc taatagtacc gccatgttta
    10501 aatcacaatc attagcctat ttattctata aacattcatt tattttaaat gacaagggat
    10561 atggttcggc aatcgttatg ttccttttag tgattatatt aattattact gcaattcaaa
    10621 tgaagttgca gaaaaagtgg gtaaactatt aaggaggcta tcaaattatg aaaaataaat
    10681 ttttttcaaa tgcgccaatc catatcatgc ttattttagg tggtattgca atggcattac
    10741 cttttatttg gatgatttta acttcaggga aaacgttggt tgaatctacc caaattccac
    10801 ctacaatttt ccctgagaaa tttcagctaa gtaactatca acaagtactt gatttattac
    10861 cattcatggc tttttatatc aatacatttt taatggttgt tggacgagtg attggctcgg
    10921 ttttattttc agcaatggct gcatatgctt gtgcgcgtct aagttttcca gggaaaaata
    10981 tccttttcag cttagtactt attcaaatga tgattcccgg ttccattttt attactccac
    11041 agtatttatt agcacaaaaa ttgggtattt taaatacaat tccagcatta atattaccag
    11101 gggtagttag tgcgtttggt acgtttttat taagacaatt tttcttaggt ttacctgatg
    11161 aattggagga agcggcctat ttagaaggtg ccaatatttg gacgatcttt ttcaaaatta
    11221 tgttgccgct tgctaaatca ggattggttg cacttggaat attcacagcg ttgtttgcat
    11281 ttaaagattt aatgtggcca ttgattgtca atacttcaat tgataagatg cctttagctc
    11341 cgggattagc aaacttacaa ggacaatatg ccacgaactt cccacaatta atggcaggtt
    11401 cgttattagc aatttggcca atagttgtga tattcatcgt tttccaaaga aaattcattg
    11461 aaggaattgc tacaacaggt agtaaattat aaaactatta attagtgatt ggagagtaag
    11521 tacatgacaa aaattacatt tattggtgca ggaagtacta tttttgcaaa aaacgtatta
    11581 ggcgatgcga tgttaactcc aagtctacaa gatgcacata ttgcattgta cgatattgat
    11641 gaaaaacgac taaaagaatc aaatttaatg cttaaaaata ttaataaaaa ttcgaatgaa
    11701 aatcgtgctg atattgtttc ttattatgat cgcaaagaag cgttaagaga ggctgatttt
    11761 gttatcaatg ctattcaagt gggtggttat aaaccttcaa cagtgattga ttttgatatt
    11821 cctaaaaaat atgggttgcg acaaactatt ggtgacacca tggggattgg tgggattttt
    11881 agaaccctac ggacactacc agtaatgtta gactttgcta aagaaatgga agaagtttgt
    11941 ccaaatgctt ggttattgaa ctatacaaat ccgatggcga tgttaaccat gggtgtttta
    12001 aaagcgacga gtattaagac tgttggttta tgtcatagcg ttcaagtatg cgtaccagaa
    12061 ttgtttgagc atttaggaat agccgatcaa tatgacttaa atgactttca atggaaaatt
    12121 gcaggcatta atcatatggc attcttgttg gaaattacaa aaaatgggga agatttctat
    12181 ccaacaattc gtcagttagc tgcagaaaaa ccaaatccac atccagattc tgtacgtttt
    12241 gaattgttga aacgttttgg ttactatatt acagaatcaa gtgaacacaa tgcagaatac
    12301 catccatact ttattaaatc aaaataccca gaattgattg acgaattagg gattccgttg
    12361 gatgaatact tacgtcgttg tgaacaacaa attaatgatt gggaaagcat gcgagaggac
    12421 attgtaaata atggggagtt aacacatacg agaagccgtg aatatgcttc atatatcatg
    12481 gattctatta cgacaggaac tccgtcagtg attgcaggaa atgtcttaaa taaaggctta
    12541 atcacaaact taccgacaga tgtttgtgtg gaagtacctt gtttagtcga taaaaatggc
    12601 gtgcaaccaa cctatgtcgg tgaattgcca acacaattag ctgctttaaa tcggacgaat
    12661 atcaatgttc aagaactgac agtagaagcc gctttaacac tagataaatc aaaaatttat
    12721 caagcagcgt atttagatcc acatctatca tctgaattgt ctttaacaga tatcaaaaat
    12781 ttagtggatg atttaatcga agcacatggg gactatttac ctgaatataa ttaaaaagaa
    12841 gctgaaagag taaggaattt tcaattcctt actcttttct tagttaaatt tagtaaaaat
    12901 attgacaaaa cacttagtaa acagttacat tagtgttaac aaaaagaaaa cgcattcaaa
    12961 ttgtataagg aggagcataa aatgaagtgg tctaaaaaaa ttattttagc ttgtatagca
    13021 ctaggagcaa cgatctcttt gactgcttgt ggaaaaaatc aggaagatgc aagcggtaag
    13081 gtagaaatag agttttttaa tcaaaaaaaa gaaatgactc aaacgattca ggagattgct
    13141 aaagattttg aagcaaaaaa ccctgatatt catgtaaaag ttgtggatgt accgaatgct
    13201 ggtgaagtaa ttaaaacacg tatgcttgca ggcgatgtac cagatgtcat taatctttat
    13261 cctcaaagta tagaattgaa agaatgggcg aaagctggtt atttagagga cttaaccgaa
    13321 gaaccgtatt tagagaatat caaaaatgga tatgcacaac gttttgctat tgaggatcgt
    13381 gtatatagca taccattaac cgctaatgtc tatggttttt actataacaa aactgctttt
    13441 gaggaaatgg ggattcaagc accagaaact tgggcagaat ttgaaaaaat agtagctgag
    13501 attaaagatc aaaacaaagt accgtttgcg attgcaggag cagaaggctg gactttaaat
    13561 ggttatcacc aactagctat agcgactgct gctggtggtg aagaggaagc gaacaatgtt
    13621 tggcgtttct cagaagttaa tggaattaat gctgaaagca aagaaatgca aaaagatttt
    13681 aatcgattag atttgttaag agaaccaaaa gctttgcaaa acaactggca aggtgccggt
    13741 tataacgata cagtagttac ttttacaaaa ggtgatgcgt tgattatgcc gaatggttca
    13801 tgggcaatgc cgatgattca ttcacaaaat ccagattttg aagtaggaac ttttcctttt
    13861 ccagcagatg aagcaggtca aagtctaacg attggcgcag gagacctagc cttatcagtt
    13921 tctgcaactt ctcaacataa agaagaagcc aaacgttttg tagaatatat gacaacacct
    13981 gaagcgatgc agaaatatta tgatgtagat ggtgcaccgt gtgctgttga gggcgttatc
    14041 gagaatactc aggattcacc attaagtggt ttaactgaac ttgcttttac tgatcgtcat
    14101 ttagtttggt tagccaaaga ttggaatagt gaaaatgatt tttatacctt aacaacaaat
    14161 tatttacaca atggcaatca aaaagccatg attaacgcat taaatgcttt ctttaatcca
    14221 atgaaagctg acggaaggta ggaaaataaa atgaaaataa aagaaatttt aaatcgttac
    14281 tgggcaatca tttttgtagc aattccaatt attttgcaga tgattttttt ctactttcca
    14341 atggttcaag gtgcttttta cagtttaact aattggactg gtctgactta taattttgat
    14401 tttgtgggtt tgaacaatta taagattctt ttttctgatc cgaagtttat caaatcgatt
    14461 ggcttcactt taattgtgac gctttgcatg attgctggac aaattggttt tggtattttg
    14521 attgcgcggg cgttgaacgc aaatattaaa gggaaaacat tctttagagc atggttcttt
    14581 tttccagcgg tcttatcggg cgtggttgtt tctttagtat ttaaacaaat ttttaactat
    14641 ggtttaccaa aaattggtag tatgttaggc attgaatttt tacaaaccag tcttttagga
    14701 acaacatggg gagcgatttt tgcagtaatt tttgttttac tttggcaagg aattgccatg
    14761 ccagtcatca ttttcttagc gggttttcaa agtattccac aagaaattat tgaagctgct
    14821 agaatggatg gggcgaatga taagcaaatt ttttggaata ttgaaatcac ctacttatta
    14881 ccaagtattt caatggtatt tattatggca ttaaaaggtg gcttaacagc atttgatcaa
    14941 atttttgctt taactggtgg tggaccaagc gatgccacaa catctttggg gttacttgtt
    15001 tataattatg cttttaaaaa caatcagttt ggttatgcga atgctattgc tttagtctta
    15061 tttatcttaa ttgggattgt ttcactggca caattaaaat tatcgaaaaa atttgaaatc
    15121 taggaggttt cccaatgaaa acatcaaaag aaaaaaatat ttctaaatac ttgcttttat
    15181 tattaggggg cttcatgatt ttagttccct tagtttcaac gctattcggt tcatttaaaa
    15241 caacacccga tattatgaat aatttctttc gttttcctaa tccagcgact ttggaaaatt
    15301 tccgaagatt gttagcggat ggaattggtc attacttttg gaattcggca atcatcacaa
    15361 tcattgcagt agttgctgtg acagcaatta ttcctatggc tgcttattca ctagcaagaa
    15421 aaatgactcg tgggaaagca tacggattga tgtatttatt tttaatttta ggaatttttg
    15481 ttcctttcca agtaattatg attccgatta ccgttatgat gagtaaaatt ggtttagcaa
    15541 atatgtgggg attaatctta ctctatctca cttatgccgt accacagaca ctatttttat
    15601 atgttggttt tattaaacta aatgtgccta tcagtctgga tgaggctgct gaaattgatg
    15661 gggctagtca ctttacaact tatttcaaaa ttgttttccc aatgttaaaa ccaatgcatg
    15721 cgacgactat tattcttaat gctctatggt tttggaatga tttcatgcta ccattattaa
    15781 ttttaaatcg tgattctaat atgtggacac taccattgtt tcaatataat tatacgggac
    15841 aatattttaa cgactatggt ccaagttttg cttcctatac agtaggaatt gtcacgatta
    15901 cactagttta tcttgctttc caaagaagta ttatagctgg aatgagcaat ggagcagtga
    15961 aataaccaat aaattattct cattataaag gagttcataa acttatgatt atatttgaca
    16021 atcacacaca aacatttcat ttacaaaata aaaaaattag ctatctatta ggaatcgaag
    16081 aaaataatta tttaacgcat ctttattttg gaaaaaagat tacgcactat tctggtgggt
    16141 atcgctaccc ccgaacagat cgttccttct cacccaacaa tgcaaatgcg caagatcgtt
    16201 tattatcttt agacacatta ccattagaat ttccaggata tggacatggt gattttagag
    16261 aaccggcgca taatattaaa ttaggaaacg gtagtcgtgt aaatgatttt cgttatgata
    16321 gctacgaaat tcaaaaagga aagcccagct tagcgggatt gccagcctta tatacggaag
    16381 atgatgatga agctgaaacg ctgattattc aattgaaaga cgcgattagt ggattgactt
    16441 tacagttgtt ctacacaatt tatcaaaatc acagtgtaat tattcgttct acgcaattag
    16501 taaataatag tgataaaaca gtggagatta accgcttagc tagtcaatca attgattttc
    16561 caaatcgtcc actcgagtta attcatttaa atggtacctg ggcaagagaa cgtcaaatga
    16621 ctagagaaaa aattcataca gggataaaag tattagatag taaacgcgga tcaagcagcc
    16681 atcaacaaaa tccgtttgtt gctttagtag atgccaaaac agatgaattt caaggagagg
    16741 catatggatt taatttagtt tatagcggta atcacgaaac ggtgattcaa aaagatccat
    16801 ttgaccaaac acgggtcatt atgggaatta attcatttaa ttttggttgg caactaaaaa
    16861 ctggcgactc atttcaaacg ccagaagtcg tgatggttta tagtaatgaa ggtctgaata
    16921 cgttatcaca gacgtatcat gaattgtata atcaccgttt aattcgtgga aactatcgtt
    16981 taaaagaacg tcccacgtta atcaacaact gggaagcaac gtattttgat tttaacgaag
    17041 aaaaaatcat gaatattgtc gatgatgcga agaacttggg cattgaaatg tttgttttag
    17101 atgatggttg gtttggcggt cgtgaaaatg acttcacttc attaggtgat tgggttgaaa
    17161 cagaaggcaa acttgcttgt ggtttggaaa atttagccaa gcaagtgcat gataacggga
    17221 tgaaatttgg gatttggttt gaaccggaaa tgatttctga agatagtgaa ctatttcgag
    17281 cacatccaga ttgggcattg ggtattccag gtcgtgaccg ctcaattagt cgaaatcaat
    17341 atgttcttga tttttcaaga caagatgttc gtgataatat ctatcaacaa atgacggcga
    17401 tttttgatcg tgtaccgatt gattatgtga agtgggatat gaatcgaaat atgacagaag
    17461 tctattctgt cttgttggac tcaacgatgc aaggtgaagt ggctcatcgt tatatgttag
    17521 ggttatatga atttttagaa aagttaacaa cgaattatcc aactatttta tttgaaagtt
    17581 gttcgggtgg aggcggtcgt tttgatgcag gtttcttgta ttacatgcca caaacttgga
    17641 cgagcgacaa taccgatgcc gtggctcgtc tgaaaattca atatggaacc agcttagtgt
    17701 atccaatttc aagtatgggt gcgcatgttt cagcagtccc aaatcatcaa acaggaagag
    17761 aaacgagttt aactattcgt ggcaatgctg cgatggctgg tgtttttggt tatgagctag
    17821 atctaacgca attaacttct gaagaaaaag cagaaattaa agaacaagtt gccttttata
    17881 aaaagcatcg ccaacttttg caatatggaa ctttttatcg cttggaaagt ccgtttgaag
    17941 gcaatgatac tgcatggatg tttgtctcaa aagaccaaac agaagccatg gtcttttatt
    18001 tccgagtatt agcagaagct gcgcatccat tagtcacatt gaaattagca ggattagcag
    18061 aagaagcaac ttatcaaatg gaagatgtaa catttgctgg cgatgaattg atgaatctcg
    18121 gtttttatat tgactcagaa ctacatggtg attatgcgac acaacgcttt tatttgaaac
    18181 agatatagta gcttgaattt acaatataag tgcaacacca aaagagactc attccaaacg
    18241 aagaaaatat gttttattta cacgcattta ctaaaaagca gcctgatttg aattgggaaa
    18301 atgaagaagt acgtgaagaa atctatgcaa tgattaacta ttggttagat aaaggactag
    18361 gcggatttag aatcgatgcg atattaaaca tcaaaaaaaa actagagtat ggcatctttg
    18421 aatctgatgg agaagatggg ttagccttca ttgggccctg gattcttaat cagtcgggca
    18481 ttgaagtgtg gttaaaagaa atgagagagc ggacatttaa accacataat agtatgacgg
    18541 tagcagaagc cgatgttcct aatgaacgtt tagatgaata tattggtgaa gatggctatt
    18601 attcgatggt ttttgatttt agttatacgg atattgatgt acctgaaact ggtgaatggt
    18661 ttaaagatag ccaatggaca tggacggata tgcgtaccaa tatttttacg aatcaattag
    18721 ttacgcaaga taaaggttgg ggcgccttat atttagaaaa ccacgatcaa ccacgttcaa
    18781 ttaataaata tattccagaa gaatggatca atgattatag taaaaaaatg ttggcgacgc
    18841 tttttatgtt actaagaggt acccctttta tttatcaagg acaagaacta ggaatgacca
    18901 acatagaaat ggcatcatta gaagattttg atgatgtagc tacgcattct cagtataaac
    18961 gggcattaga atatggattg gcaccagagc aagcattgaa agctgtcaat aaacgaagtc
    19021 gggataattc tcgcacacct atgcaatgga atacgcaaaa aaatgccaga ttctcaactt
    19081 ctgataatgt atggttaaaa gtaaatccta attatccaga actcaatgca gaagctcagt
    19141 taaccaatca acagtcggta ttcaattatt atcatcaatt aattaaatta agaaaaaata
    19201 gtgtatacaa agacgtgcta atctacggac aatttctacc agtgaaagat aaaaatgaac
    19261 atattttgct ttatgaacgc caattagaag ataaaatcgt attagttatc ttaaacgtta
    19321 cagcaacaga acaagaatat tatgtagatg aacagtacca acaagtatta atcaataatt
    19381 atgaagatgt tttattagat caaagtcgaa aattgcgttt acgaccgttt gaaagtattg
    19441 tattagctaa ttatggagga caattatgaa aattaaaaac gaagcaatgc tcatcaccta
    19501 tgcagatagt ttaggaaaaa acatgggcga attaaaacaa gtcttagaca aacatttaca
    19561 aggtgttatt ggtggtgtac atctgttgcc tttttttcca tcgacaggtg atcgaggctt
    19621 tgcgccgagt gattatacga cagttgatcc agctcttgga acatgggaag atgtggaagc
    19681 attaggtgaa gattattatt tgatgtttga ttttatgatt aatcacattt ctcgtgaatc
    19741 aaaatttttc caagatttca aaaaaaacca tgagcagtca ccatacaaag aaatgtttat
    19801 ccgtattcat gaatttttcc cagaaaatcg accaacacaa gaagatattg atttaatcta
    19861 caagcgtaaa gacaaagcgc cgtttcaaac ggtagaattt gcggatggta cgacagaaga
    19921 ggtttggaat acctttgggg aagaacaaat tgatttagat gtgaccaaag aagttgtgaa
    19981 agaatttatt cgagaaacca tcaaagatat ggcgaaccat ggctgttctt taattcgatt
    20041 agatgcgttt gcttatgcga ttaaaaaatt agataccaat gatttcttcg tggaaccaga
    20101 tatttgggaa cttttagacg aagtgcgtca agaagcagcg aaatatgacg tcgaattatt
    20161 accagaaatc catgagcatt attccattca aatgaaaatt gccaatcatg attattatat
    20221 ctatgacttc gccttgccaa tggtcactct gtattcactt tatagtggaa aatcagaacg
    20281 tttagccaat tggttgaaaa tgagtccgat gaagcagttt acaacattgg atacgcatga
    20341 tggaattggt gttgtggacg ctcgtgattt attaacagat gaagaattgg attacacctc
    20401 tgaggaattg tataaagtcg gggcgaatgt gaagaaggtc tattcaagtg ccaattacaa
    20461 taatttagat atttatcaaa ttaacagcac ctattatagt gcattaggcg ataatgaccg
    20521 tagctatctg ttagcacgtg cgattcaagt atttgcgcca gggattccac aaatttatta
    20581 cgttgggtta ttagcaggaa aaaatgatat tgaactgttg gaagcaacga aagaagggcg
    20641 taatattaat cgtcattact atgatttagc cgaaattgaa gaagaagtgc aacggccagt
    20701 gattcaacaa ttatttgcac ttttaaaatt tagaaatgaa tctgcggcgt ttgatttgga
    20761 tggaacgatt gaagtagcaa cgccatcaga atcagaaata gtgattacac gtaaaaataa
    20821 aaacggtagt gatagtgcaa cattaacggc taacttggat acgaaagaat ttatcattga
    20881 gtctaaagat ataaaaattt tcactagtgt tgcattaaaa ttttaaccta ttccatagaa
    20941 caaaaaatcc tttatactag cacttggatg acttatccaa gaccaataaa aaggactcat
    21001 acatggataa gtataaaaca aatacagcat ttaataaatg gttttctgcc ataaaattag
    21061 gaaaactttc tgtcaactcc caaaaatgta tttttgatta caattcgtat cgtaaaaaac
    21121 tgtcttttga agctgtgctc aagctctttc tctacgcagt taatgaagaa aaagaaagtt
    21181 tgcgtgattt gagtacagct cttatcgacc gatcgctcca actagaaacc gatgtgtcat
    21241 cgattagtca tacccaattg tctcgagcat ttaatgcact tgaacctaaa gtgttagaag
    21301 aaatttttca acaactgtta gaaaaagttc aacatcaaat caaaccaacc aagcgaaatc
    21361 atctgtatct gattgattcc agtacgtttt cgcttagttt aaaacgtcat aagtgggcga
    21421 ctttccgtca aacaaaatca ggtgtcaagt tacacttaaa ctattgttat atggacgata
    21481 ctacgatgta tccaactgat tttaccttaa caaatgccca tgaacatgat ctcaatcaac
    21541 taccagtatt ggtgaatcaa ccagaggcaa cttacgtgtt tgatcgtggg tattaggact
    21601 ttgaaaagat ggatcagatg cattgggatg gctatttctt cgtcactcgg attaaaaaaa
    21661 acaccaaagt tcacgtgata gatactctgg aaacatcgcc agaaacagaa attctacgag
    21721 acgagctggt tcgtttagga tctaaaacct atctaaccgc caatttccga ctagtcactg
    21781 ttcaagataa gaacggacgt gtgtttcaat ttatcaccaa tcgaatggac gtctcttcaa
    21841 aagaaatctc tgatatgtat cacgctcgtt ggcaaatcga attgtttttc aagcacatca
    21901 aacaacacat gaccattaaa accttctttt ctcaaagtga aaaaggcgtg cagaatcaat
    21961 tgattttaac gatgatttcc gctctcctca cgtttctcat caaattggag acgaaaacgg
    22021 aaaaatcagt gttccaaatc aaacgattct ttcgttattt actgtttcag ccctttgaat
    22081 gttgtctaga aaaattaatt cccacatgag aaaaaagaaa tttggctcgt tacttttggt
    22141 tttcagttga ataacagata aaaaattttc tggaaaaagt catcctctgc gtcagcacag
    22201 ttttttttga cttattttca gaggaaatag ttttcagaat attctaaaat gttttatgca
    22261 acactagtga tattttttag aattagattt ctaatgtgca ttttttgagt attaaggagc
    22321 aagtatacaa agatttataa agctgattaa atttgaataa aaatatgcag gaacgctact
    22381 atttttagta gtgttctttt ttatatttga ataaaaaatg tggtggttaa atggtggtta
    22441 taacaaaaaa tgaattaatg aaattaggtt atgggaagta tcagtctgag agaatcatac
    22501 gacaggcaaa agcaattatg gtttcaaaaa gctataccta ttacgaaaat agaagattag
    22561 gactagtacc tgttgaatct gtagaagaag ttttaggtat gaagatagag gtagaggagg
    22621 attttcagaa tcatggttaa ttttaaaaaa gataaaaatg ggaaatatca ttttactgct
    22681 agtttgggat tcgacaaacg aacagggaaa agagtacaaa aaaacgtaga ggggttaaaa
    22741 cagttaaaga agaaaaaaag cgtataccga acttgtttct aattatggaa aagaagcaga
    22801 aacttctcaa agtactatgt tgtttgagga gtttttttat aaattattcc taccttatta
    22861 tcaagggaaa gtagaagaaa gaacatataa aaatcggtta tctacaatca aaaaaacttt
    22921 tcttatttct ttaaaatgga gttaagagaa atacgtccaa ttgacattca aaagtggcaa
    22981 aataatttat tgagcaaata taaaaattct tatgtaagaa acatgtatgg gatttttcag
    23041 atgtcattag atcgagcagt tgttttggga ctacttgaaa aaaatcctgc aaaagttatt
    23101 ggaaatgtga agaaaaggaa aactacagtt gatttttgga caaaagaaga atttacaaaa
    23161 gttatttcaa ctttttatat tgatgatttg taccaacatt ttggttttat ttgtatgtgg
    23221 ttacttttta tgaccgttat gcgtttggga gaagccactg cattaacttg gaaagatatt
    23281 gattttgaaa ataaaaactt gcgtgtgaat aaaacactct attataagag agccaaaacc
    23341 tatgagtttg tagaacccga aacggcttct agtattcgta caatcgaact agacgacaat
    23401 acaattacct ttttaaaagc ttggaaggag cgtcaagttt caataggagg catgtcgttt
    23461 gtactttctt ataatggaac acctactcag aaatatacaa tccgtcatat tatgagaaga
    23521 catgcaacga tcgttggtgt tcatataatc cgcatacacg gattaaggca tagtcatgct
    23581 tctttgctta tatcacaata aaaagatgca cttcgaatta aagaaaggtt aaaacatgct
    23641 gatgtacaaa cgactttagg aacttatggt catttgtatc taaattcatt gagagagata
    23701 acaaatgatc ccacagatta tatccaaact aatttttcta aaatcaaacc gacacctatt
    23761 tacgataata aaaaataaat gataaacaaa agtaggatca caccaaaaga aacacactag
    23821 aaaaactata atacaggctt ttctagcgtg ttgtttctta tttccgccta tttatacatt
    23881 atactcataa agcatttaat gcatcgatcg tagccgcatt gtctatatgg gtatataaag
    23941 ttgttgtatt ggtacttgaa tgtcccaatt gttgggcagt taataccaat gaatttgtct
    24001 gttggtagag tctagtagct aatgtatgac gcagtttgtg agggctgact tgaattctaa
    24061 agccttttga atattttgct accatccgtt cgattgcaat accggatata cgcttggctt
    24121 ctccttggta aattgctaga aataaagctt tttcgttttc tgttacttta tatcgttctt
    24181 tccgaatagc tagataatta gctaaatatt ccattgcgaa tgctgctata ttgacagcat
    24241 ctcttttatt tccttttcta gttactacca cattattctt atctaaagat aggtcatcta
    24301 aattaatatt tataacctcg gagagtctca atccagatcc taaaatcagt gcattgatcg
    24361 ccaggtctct ttctacatct cgttgataat agatcaaagc tcgtttactc aaagtatttt
    24421 tatattcatt ggcaatataa tctagataat tttgtgtatc cttatctaga aataattttt
    24481 tctcaatggc attggcacgt gcagctaacg tagttttatc tgtgatggtt gctacttgat
    24541 ccataggatt attgtctaag tatggttttc ctagaggatt attggaactt ttcgaaagat
    24601 atttaaatag aacctttaat gccgtgatat ttctattgat agtagaagta gatagaccta
    24661 agttattatt atctttgagc ttagtttttg ttgtctcttt ttttacattt tcttttgtgc
    24721 gacgtttcaa atgagatttg tacaagttta ggtcataggc agttaactgt cttatcgagg
    24781 tgatagaagt atcaggaacg atagactcac tcagaagcca ttcataaaag agcttataat
    24841 tttttagata ttctaaagtg gttcgaggtg attgatcttt atcctcacga tcttgaacaa
    24901 aatcttttat ataatcgggc atatgaagta tgattgtatc tattttttga tatatttctg
    24961 tatttttcat gtcattcatc ctttcaataa gtaggggaga agagtatctc tataattgga
    25021 ctataccaat tatagaatgt tttgtcaatt ggtatgctct aaaatttatt aatttgtttc
    25081 aaaaaaagca tataaacagg tatacgttta ataatttaaa ataaatggta tacataaata
    25141 gggaaggaaa tagaagggag caatctaaat tttttgaatt ttcttaatta attatataga
    25201 aagaacaatc tttctataga gttagacatg agagggacta tgcttgattt aaaatcaaca
    25261 agcgtatata ctgtatatac aaagaaaaag gagggagaag actatggcaa ttaaagaaag
    25321 aaaatttagg gaagtgggga actcagtagt attaactatt tcaaaggagt ttcttaaaga
    25381 aaatgggtta aatcccggag atacggtttt tttagatgaa gaaaaacttc gagcggcaat
    25441 tacaaaaaaa caagccttat ctgatgaaat tgatttagca atatctcaag cattaactga
    25501 atatgatact acttttagag agcttgtcga taaatgagta ttcattacct agaagccaaa
    25561 gatattataa aaatgaatgt aatccaaata aaaaaatact ctcctaatga accccttggt
    25621 gtaaaggatg cgaatgcttt agaaatgtgt gtagggcaac ttcgagcctc agcttttggt
    25681 gaagaggttt atccttcgat ttatgaaaaa gcagctatcc tacttataca attgataaag
    25741 aaacatcctt ttcacaatgc aaataaacgt actgctttct tagctacttt tgtctttttg
    25801 aaaatcaatg gacagctttt agctattgat caaaaagatg ctgtaaattt agcggtatat
    25861 attgctacgt atgataagga tttcgatcaa ctaaaatatg aagtgatcaa cactataaag
    25921 acacatacgc agcccattta aggagttact gtatgtgtct ttatagggtt attagctgct
    25981 atagcgaaat aattgaagta aattacaaat agctataatt caaatccaac tatatagaaa
    26041 acgtaaattt tctatatact tggatatgct ggaatgttta agagaataat tacatttcta
    26101 gaaaatacaa taaaaattac aggataatta gccatatcct acctcctcta gcagttgctc
    26161 aaaagttagc actaagtaac ttcttttttg ttgctggtaa caatttatca atactaaaat
    26221 agcgtttaag tatctaataa atactaattt taaagctctt aattattatg aaacacgata
    26281 taacaatatt gctatatcgt gctattgtaa tattatacat ttgaagacag cgtattcttg
    26341 ttctaatatc aggaattttt tataaatatc agtcatatta agactcaaaa tttattggta
    26401 tgttcctctg tatagagttt gaccgtctac cattatatag caattattat cattgttgtc
    26461 ttgcaccaat ataccgttaa gggtagcact aggagtagta acaatcactg gaaactcatg
    26521 agaaagctcg gtcatttttc ctgttctgct agaatcgtgt tctattgtca agccatttac
    26581 tataaacgtg aatactttac ttcctattgt tatagtagct ttttgagaaa cagtttctgt
    26641 agctggagct gtgcttcttt ccgaatttgt tagggtttca ctggattcat tgctataaac
    26701 aattgtagag gattctacgg cttcttgagt gtccacgttg gtgctgctag cttcatctag
    26761 cttccttttg tattgttcgt tactgattgc taagctttcc cgttggtcat caagcttatt
    26821 catttctgta atggtataaa ataatcctcc tgctagtgct atattaacaa ctaaactaac
    26881 cgcaaataat acctttttca ttcttccttc tcttctttct ctttttgtaa ttccttactt
    26941 ttggataggc tacaattaac tagacagaaa aattaaggtg tgtagactag aagaaaacat
    27001 accaggagga atttttatgt ctaagagaac acgaagaact ttttcacaag aattcaagca
    27061 acaaatcgtc aatctttact tag
//
LOCUS       DACGLW010000031        26720 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.031, whole genome shotgun sequence.
ACCESSION   DACGLW010000031 DACGLW010000000
VERSION     DACGLW010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26720
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.031"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            complement(18..710)
                     /locus_tag="IXT53_001360"
     CDS             complement(18..710)
                     /locus_tag="IXT53_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ4905197.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            705..971
                     /locus_tag="IXT53_001361"
     CDS             705..971
                     /locus_tag="IXT53_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ4905198.1"
                     /translation="MHRTFGSRGCPTLHLADRYAERKTPTLCFAFSARTRQAGIPLED
                     IKDFLGHKDVSTTQVYAHISPEVKKRSMNQLENYIEEQIKKHSN"
     gene            complement(1147..1716)
                     /locus_tag="IXT53_001362"
     CDS             complement(1147..1716)
                     /locus_tag="IXT53_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ4905199.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYKTLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1790..3079)
                     /locus_tag="IXT53_001363"
     CDS             complement(1790..3079)
                     /locus_tag="IXT53_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905200.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFYFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLIKVLYQEWLRYVI
                     GIHDRLIEEVRYYQETLEKFPNSKDKLYQFRLAIQQALAHPAVEWGMLLLKTLQERGY
                     LDVHPPEVKIVFFFRYEHQEARRLAGVLNKTLRHRKRISIHVCTRNKPPRYADLVITD
                     HFFPLDHNEDQKTYLYHPSFGIERLLTDINYWLGKNR"
     gene            3433..4281
                     /locus_tag="IXT53_001364"
     CDS             3433..4281
                     /locus_tag="IXT53_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HAQ4905201.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4426..5367
                     /locus_tag="IXT53_001365"
     CDS             4426..5367
                     /locus_tag="IXT53_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HAQ4905202.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTAYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5357..7528
                     /locus_tag="IXT53_001366"
                     /pseudo
     CDS             5357..7528
                     /locus_tag="IXT53_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(7640..9811)
                     /gene="copB"
                     /locus_tag="IXT53_001367"
     CDS             complement(7640..9811)
                     /gene="copB"
                     /locus_tag="IXT53_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HAQ4905203.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIK"
     gene            complement(9831..12017)
                     /locus_tag="IXT53_001368"
     CDS             complement(9831..12017)
                     /locus_tag="IXT53_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HAQ4905204.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGWIAVADQIKLEAKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLSLNRHMKK"
     gene            complement(12017..12226)
                     /gene="copZ"
                     /locus_tag="IXT53_001369"
     CDS             complement(12017..12226)
                     /gene="copZ"
                     /locus_tag="IXT53_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HAQ4905205.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(12239..12679)
                     /locus_tag="IXT53_001370"
     CDS             complement(12239..12679)
                     /locus_tag="IXT53_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HAQ4905206.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYHPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12753..13292)
                     /locus_tag="IXT53_001371"
     CDS             complement(12753..13292)
                     /locus_tag="IXT53_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HAQ4905207.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQSFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
     gene            complement(13907..15373)
                     /locus_tag="IXT53_001372"
     CDS             complement(13907..15373)
                     /locus_tag="IXT53_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ4905208.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
     gene            complement(15684..17765)
                     /gene="cadA"
                     /locus_tag="IXT53_001373"
     CDS             complement(15684..17765)
                     /gene="cadA"
                     /locus_tag="IXT53_001373"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HAQ4905209.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQEIKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKIFMQYVASAEQFSTHPIAQSVLEGYAGPLLP
                     TANIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPSSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKKAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKNKR"
     gene            complement(17907..18086)
                     /locus_tag="IXT53_001374"
                     /pseudo
     CDS             complement(17907..18086)
                     /locus_tag="IXT53_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308695.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
     gene            complement(18289..18648)
                     /locus_tag="IXT53_001375"
     CDS             complement(18289..18648)
                     /locus_tag="IXT53_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HAQ4905210.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(18678..19019)
                     /locus_tag="IXT53_001376"
     CDS             complement(18678..19019)
                     /locus_tag="IXT53_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905211.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERLDQRPSGKLIQHTLVTKENTEEIQKKFAEYLILEAK
                     KRRIKSFFAQF"
     gene            complement(19016..19690)
                     /locus_tag="IXT53_001377"
     CDS             complement(19016..19690)
                     /locus_tag="IXT53_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ4905212.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     EFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTVGICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIVYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(20743..22041)
                     /gene="asnS"
                     /locus_tag="IXT53_001378"
     CDS             complement(20743..22041)
                     /gene="asnS"
                     /locus_tag="IXT53_001378"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HAQ4905213.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(22081..23271)
                     /locus_tag="IXT53_001379"
     CDS             complement(22081..23271)
                     /locus_tag="IXT53_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ4905214.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYSLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(23292..23819)
                     /locus_tag="IXT53_001380"
     CDS             complement(23292..23819)
                     /locus_tag="IXT53_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ4905215.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEEADHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(23866..26655)
                     /locus_tag="IXT53_001381"
     CDS             complement(23866..26655)
                     /locus_tag="IXT53_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ4905216.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLIQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
BASE COUNT         7479 a         5693 c         4704 g         8844 t
ORIGIN      
        1 aaagaggggg ggatttttta tcgttgtgga atatttaaat accaacgctt gtcatgaaaa
       61 tcttgtgctc cacccttagt atttccttct ggatcgtttg tcgcacgcat catgacgtat
      121 actttcttat tagggaaatc acgcatattg aaagatacat gatagccaac atttcctaaa
      181 gtattataag cttgatttac gtctggtcta taaatcccat cagctcttac tctagctagt
      241 tcttttccag tattgtagtc cataatgaaa atatactcgt atttataatt agcaatgtgc
      301 catccagcta catgcaagtt tgcgttttcg atttctccga actgatcaat gtgggcgtaa
      361 tttgttccat ctgtcagcgt agaatttgca gcacctgctc gagttggatc aattactggt
      421 ttatcctccg aagtagttgg attatcatcc gtaaaaccat gagccaaatc ataggctagt
      481 ttttctttac ttacacccat ttcagaaaga taaccgtaag gatctgtatg atcgccccaa
      541 atattttgtg ttacccataa atgcgatttg attcctggtt ggttataagg agtgtctaat
      601 gttaatggaa taccatattt cattgctgaa tctcttgcca attcaacgta tactttatag
      661 ttcttttcaa aagtttcttt atcatgtgtg tgttgtaact cgatttgcac aggacttttg
      721 gctcacgagg ttgtcctact ctacatcttg ctgatagata tgctgaaagg aaaacaccaa
      781 cattatgttt tgctttttca gctagaacga gacaagcagg aataccttta gaagatatca
      841 aagatttttt aggtcataaa gatgtttcca ctacacaagt ctatgcccat atttctcccg
      901 aagtgaaaaa aagatcaatg aatcaactag aaaactatat agaagagcaa ataaaaaagc
      961 actcaaattg agtgcttttt taagctcact atcactaaaa ctatcactaa ttttatttat
     1021 aagcgaacat gtttctatca ctgatttttt aaatctagcg aaaaaccctt atcttaaagg
     1081 cgttcgttca attctttggc aaggtcttca aagcctggct tgcctagaag tgcgaacatg
     1141 gttttcttat agctcatgta gaaaaatttt cttgatgatg attttatcca tcaattgaga
     1201 ttgactgttt tgctctccta tgatttcagc tatgattgtc cgattttgcg gttttcttct
     1261 taactggtag ttgattttcc cagtttccat caactcgcta agcatatcca cgactccgcc
     1321 aaatccggtc tttttcgtaa taaaacctag tgctaccatt cgttcatcta gattttttgc
     1381 tgtaatcgtc acaccagtaa tgagcgagtg ctgtaatgtt tgggccagct cttccatccc
     1441 ttcagcattg gatacgacgg aagctgtttt gttaataggt tcttcgttta tttcttttgg
     1501 ttctttcctc tccactttca ctgtttgacg aaggacatct agttcttgca agagcgtttt
     1561 gtattgttct tgggtcagca tcacgccagc tactttttca cgattaaata tatatacgcc
     1621 agttcccgcg tctcgcgctt gagcaaatac atccatcggc gatcgtttca cttctgtgat
     1681 cgaagatgtg ggaacttcta atttctttaa ttccaatgga tttcctcctt tactaatcgc
     1741 tttttattat agcacagact gacttgtttt gatctttttt ccaaaggttt tatcggtttt
     1801 tgcctaacca gtaattgata tcggtaagaa gtctttcgat accaaatgaa ggatggtaca
     1861 gatacgtttt ttgatcttcg ttatggtcta gtggaaaaaa atgatcggtg atcactagat
     1921 ctgcataccg aggcggctta tttcgagtac atacatgtat agagatccgt tttcgatgtc
     1981 ttagcgtttt gttcagtacc ccagcaagtc ttcttgcttc ttggtgttca tagcgaaaaa
     2041 aaaagacgat tttcacttca ggagggtgga catctaaata ccctcgttcc tggagtgttt
     2101 tcagtaaaag catcccccac tctactgcgg ggtgtgcaag tgcttgttgg atcgctagcc
     2161 gaaattgata tagtttatct ttggaattgg gaaatttttc taatgtttcc tgataataac
     2221 gaacttcttc tatcagccgg tcatggatac caattacgta acgaagccat tcttggtaaa
     2281 gtacttttat cagctttgat ttgctttcgc tctcaaattc gctattcagc caaaacagcg
     2341 aatcaagctt ttcagtcaca atcgtcaatt tccgatagtc ttgtgtctct tctatccgtt
     2401 tccaccgctt ttcagtaatc tggttacagc tcaagcgcaa aattgctaga taattttttt
     2461 cttttattgg caaaatctgc tcctctctaa atagcaattc aggaaacaat tcaaagaata
     2521 gtgttcgact gtctcctaaa atctcgattt ctgccggatc ctttctttgc ttcaataatt
     2581 ggatgaacat ctcagaaaag gactgctctc catcatctgc aaaaaaatgc ggaaaatctt
     2641 tcaaaaagta aaacagcaga tatcttactt ttctttctga accaaagatc tgtgcttttt
     2701 tataagaaaa agaaaggtgg tactcgccaa accactttct caatcgctct tgcgtagaac
     2761 gatcgtactt ctgctcgtaa ggttcattta atagcagatt tttcagtaaa caaaacgggg
     2821 gtgattggat cagaaaaaca aacaagagat aaggttcgct atatccttga tcataaacta
     2881 actgaacggt tttcttatga agaatcagct gaatgggaca gtcgctttcc gccaaacgtt
     2941 tcagatgccg atccactgtt ttaacatcga ttttcagttc ttcagctaaa tcagcttttc
     3001 gccagatcaa atctttcatt agattgatta gtgaaaataa attctgtgca ttttttgata
     3061 atagtttttg ccatctcatc gatcatttct cacctgattt ctaaacatat tgtttgatca
     3121 aaatgagttc actcctaaaa agataaaaaa ctccctttct atttatttaa atacgcaaat
     3181 cttcctcatt attttttatt ttactcaatt taatcgtatt ctcatttcaa aacgaatgta
     3241 ctttaaaaaa acataataag atcactttgc ttgtaggatg tattcccttc cattacaatg
     3301 aaataaatag cgattttatg agaaggtgat gtgaaaatgg aagaaaaaat agaagaatcc
     3361 aaacaaaaaa tcgatcagtt gatataagaa ttagaaaaag cggtcatcgg caaacgttca
     3421 actatccaat tgatgatcgt ggccctatta gcaggtggtc atgtcttatt cgaagatatt
     3481 cccggtgtag gtaaaacgtt actggtcaaa gcactagcaa aagctgtaca gggaaccttc
     3541 agcagagtcc aatgtactcc tgatctgctg cctagtgata ttcttggttt ttccgtatac
     3601 aattcacaat caaaagagtt tgaatttcgt ccgggaccga tctttaccac gatcttgttg
     3661 gcagatgaga tcaatcgtac gactcctcgt actcagtcag cacttttaga agcaatggcc
     3721 gagaatcatg caacgatcga caacaataca taccctctag acaatcattt ttttgtctta
     3781 gctacgcaaa atccaatcga gtacgaagga acatatcctt tgccggaagc acagctcgat
     3841 cgttttttat tccgactaca aatcggttat ccttcttttg atgacgaact tttgctactt
     3901 ttggataaat tcgaaaaagt gttggataac ttaaatgaag tattgaacag ttatgaaata
     3961 gaagaaataa aaacaaatgt ggcgaatgtc tttgtcagtc ccgaagtggc atcttatgcc
     4021 ttgcagttag tgtcagcaac aagaagtcat gctgccatcc agcttggcat cagtccacga
     4081 ggaagccttt cctttattca ggcagcaaaa gcttatgctt taatccatgg gagaaattac
     4141 gtcacgccca aagatttgca ggatttagtt ccttacgtgt tttcccatcg cctgattttt
     4201 tacgatcgtt cattgaagga agacgaaaag aaacaaatca ttcatactct tgttgcacaa
     4261 attcctattc ctgtgaggta gattgaggta gacaatggaa aaaatcaaag attatttcat
     4321 taattttgtg ttatttctgg tatatttcat cattgtgctt tttgctcttg tattcaacaa
     4381 ttctttaggc tggttcttgt tggccttttt tactctttat gtatcatgga gcttgatttc
     4441 attaagcagc tttctaaaga aactttcggt tacttgtaca ttgcagccac tcgtttatcg
     4501 caaaaaaagt atgcaggtta ttttttccat agcaaaaaag agaacttttt ctttacctct
     4561 ccctcgttta caaatcggct tcccagcaat ctttgcagaa aaaaacaaga gattttgatt
     4621 cttacaaata aaccgcaaga agtccaattg ctttggcagc caaaagaaag aggatgccat
     4681 gaaacattaa ctgtcatttt tacagcctat gatagtctag gacttttcag aaaaagtgca
     4741 aaaagagaga tctcttttcc tgtgactgtt ctgcctgctt tttgtaagat tcaagcagaa
     4801 aaattacaga gagttctgga aaaaaaacag cagctgaacg cgtaccaaag aggtcttgat
     4861 tttcgggaac atcgcgctta tcgtccaggt gattctttca accgaatcga ttggaaattg
     4921 tctgctcata ctcaagagtg gttataccgt gaatacgatt atcaagaaga agaatgttct
     4981 cctctttttt gcttttgggg ttcgcccagt ccaaaatttg aacctctgct agacttgtat
     5041 ttttctttat ggcatttgag aaaagaaaag cagccggaac ttctgatatt aggcaaccgc
     5101 gcctttcatg gcaaagaccc cggtcatacc tattttgctt ctttggaagc tggcgatgag
     5161 aaagcttttt tcgcacactt gaaaaaatat gcaaaaaaac aaatcgtcct ctttgttcct
     5221 gagaattctc ctgaagtctc agaagctatt gaaacattgt caaaagatcg aacggcttat
     5281 cttgtttatt tcgtagaaaa acaactcatg gtccaagaaa aagacaagat atgttcattg
     5341 ccaggaggtg aatggattga cgattaaacg ggaaaaatgg ctgcgtggcg tttactcctt
     5401 tgtgatacta agtgtaggtg ctccgatatt tgtagtagct aatcaattgc aaaacagcta
     5461 ccttcttatc ctaactattg gtgtcctatg ccttattgtc tgctgtattc ctaaaaaatg
     5521 ggtacgtttg ctgctttctt tgccagtaat gataggttgt ttgtttttat attttccttc
     5581 gaagacattt tccatattat ggatcttaca gtttttttcg tcagttacag aggaattctt
     5641 ccatctatcc caaggtaaac tttattattt gccagaaaag acagcttttt ttctcataat
     5701 gttgctgatc tatttattta ttacattgac cgtggattac gactattggt acgcaccctt
     5761 tctttctttg atggcttact tcatgatgct taccgttttt cggaaaaaag atttactgta
     5821 cgaaatgatt gcggtagtaa ctgttttgtt cgtgtatctg gcagcaaggc aatttttctc
     5881 catccggttg ttgtcgggaa acactcgatt ccagtcattg actacagttc tgcttatttg
     5941 tttcttaact tttagcgcta tcttgccttt atatcttccg aaagtacacc aatctctcga
     6001 ggaaacgagt gaaccgatcc gagaatatct taatgatgaa gggctttatg acacactcca
     6061 tgagtatcaa ttaaccggaa gtgcaagaac aggcttcagt gagaatgatg aacagcttgg
     6121 tggaccttta tatgataatg gcgaagttgt ttttacagcg aaccaaaaag gaaatggcta
     6181 ttggaagatc gaaaataaaa atcattacac cggttctgga tggatagaag atcaggacgc
     6241 acccatccaa ccaatccaag aattccctgt tgacatcacg agctaccaaa ccgttataga
     6301 agacaaagaa aacattacat tgaccctaaa aaaacaagct ttcttacctt atccttatgg
     6361 aaatactacc ttgactgatt tttcattagc tagtggacaa acgctttact atttgcctga
     6421 aagccagcga ttcattcctg tcccgaatga atcgacacgt tctgcaactt tacaaacaga
     6481 aaggttagtt tttacgagag aagaattaaa aagcagtcag ttaaaagctg atgatttaga
     6541 aaatcagcag acaaatcttc aattgccaaa aagtttgcct aaacgtattg aggacttggc
     6601 ggtcgaattg accaaaaatg aagcaacttt aattcgtaaa gtcgaagctg tctaacagta
     6661 tttaaaaaca agcggaaatt tccgctattc caaaacggat accgggcata ctccgaaaga
     6721 acacgattat gtggattatt tcctctttga ttcaaaagtc ggctattgtg acaacttttc
     6781 aacttctatg gtcgtccttc ttcgaacact tggtatccca gctcgctgga caaaaggttt
     6841 tacaaatggg ctcagaacag gcacagataa tcaagggaat gaggaataca gcattctcaa
     6901 tagtcatgct cattcttggc cagaggtata ctttcctgga tttggctggc ttccatttga
     6961 accaacccct tcatttgcca atccaatggg ttcacaacca gttgctgaaa caaccgaaac
     7021 cgtccagcct gctatcgaac aaccaaatga aagcgaaaat atggcagaaa ctatttccag
     7081 tgagagatct gaggtcattc catcagaaga aacaacactc acaccagctt ataaaatcgt
     7141 cttattcagc cttggagtgt gtttatccgt cttgttgttc tttctattgt tattcagaaa
     7201 atctctgcgc tggacaagtt atcttatcta tcttcatttt tttgcttcta ctaatacgat
     7261 tgcatatcaa aaacttctta accctttgga aacattggta ccgcgcaaac ccgcagaatc
     7321 tttgcaggaa tatagcctgc gaatagaaca agaattcaag ctagatctgc gtttcattcg
     7381 attaactact caatatgaag cagaaatata tggacgacaa gcagatccgg taaaattcga
     7441 taaaaaaacc tatttagaaa taacgcgtat aataagaaaa acggtataca aaaaaattct
     7501 gacaaaaggg agccgcttca aaaaataaga taaaatttct ggagaatcta cttctggaat
     7561 gccgagaacc agctgattga gtatcgatta ctcgaaaaaa aggttgtgac acgttttttg
     7621 tcacaacctt ttttcattat tatttgattt tcaatgtcaa agcatttaga gctacaacta
     7681 ccgtacttaa cgacatcaag acagcaccaa ctgctggact taatatgata ccaattggcg
     7741 caagaattcc ggctgctagc ggaattgcaa gaatattgta acctgccccc caccagaggt
     7801 tctggatcat tttctttcgc gtttgcttgg ctagatcaag aaaatgcaaa atatcttttg
     7861 gatcgctgtt tgtcaatacg acatccgcag aatcgattgc gatatctgtc cctgcaccga
     7921 ttgccatccc gattgaagca cgggctaaac ttggcgcatc attgatccca tcacccacca
     7981 tgattacttt tttcccttgg tcaaggtatt tctggacaac agcttcttta tcatctggta
     8041 ataaaccgcc ataatattct tcgatcccta aataatctgc tactgcttgg gcagcgtttt
     8101 tattgtcacc agtaagcatg acaggagtga tttttctttc tttcaatgtt cggataaatt
     8161 cttttgcttc tgtttttact acgtcaccta aagcaacgat tgcagctaga tgattttcta
     8221 aaatcaggaa gctgatggta tttccttgtt cttggtaagg tttcaataat gcttgttcta
     8281 cgtttaatcc gagacgctca acttcttttt cattaactat tttcacttgt tgttttttga
     8341 ctgttgcctc tagcccgact cctgaaagat tttttagatt ttcagcttga taaggcttga
     8401 tttcatgttc tttcaaataa ttcataatgc caacagccaa tggatgattg gcattcgctt
     8461 ccaaggctcc tagatattga aggatttcat ttttattgaa cgcatcatca agtacttcga
     8521 cacccgttac agtaaatttt ccttctgtta gtgttcctgt tttgtccagc atgatcacat
     8581 ctagatcatt ggcttgctcc agtgcattgc ggtttttcaa aagtaatccg tttttagctg
     8641 ctattgatgt ggagcgagca acaactaatg ggatagctaa acctagagca tgaggacagg
     8701 cgatgataaa gactgttacc atacgttcta atgcattagg aagatctgtc aagaacaacc
     8761 aggcgatgaa tgccaggata cccacaatta atgcgacata aaacagccat tttgccactt
     8821 tgtcagataa tgactctagc ttggattttt ctccttgtgc ttttcgcacc atttccataa
     8881 ccttcgctag ataaccattt tcaccagttc cagttacttc gatttcgatc gttccatccc
     8941 cattgataga accgccaatc actttgtcat tggcttgttt tttgattcct cttgattcgc
     9001 cagtcacagc tgattcgtca acaattgttt cacctttcaa gattacacca tctgttggca
     9061 ttttatcacc agaccggacg atcaaacgat caccttcgtt tacttctttc aatgaaactg
     9121 tctcttcttt tccatctttc gtcaagcgtt tcactgattc tggcagtaat tctgctagtt
     9181 tttgtaaagc attgctggca ttcgacacag cattcatttc aatccagtga cctagtagca
     9241 taataacgat caaagtagcc aattcccaga agaaatccat gacgtgtgta tgtggattga
     9301 gcagatttgc gataaacgaa tagacactat aaatatatgc aactgtaatc cccatcgcga
     9361 tcagtgtcat catagcagga cttttttgtt tcaattccat cttagctcca cttaaaaatg
     9421 gctgaccgcc atagataaat agtatagttg ctaagatcaa tacgagccaa tcagaaccag
     9481 gaaacgttac ttgaaatgga aattccattc ccatcattgg tgaaaaaaga atgattggga
     9541 tagctaggat aagcgatagc caaaattttt gtttgaaatt ccccatgtgc atggaatgat
     9601 ccatgccgcc gtgttcgtga tccatgtgca tcgaatggtc catttgtttg ctgtttgcta
     9661 tatctccatg gttatgtccc atgtgcatgg aatgatccat ctgactatgg tcatgtttca
     9721 tctccatctg tttttcatga gattctgtac tttctttgtt tactacttct tttttatgct
     9781 catgtttttt ctccatatcg ttcccattca tttttactcc atcccttctc ttactttttc
     9841 atgtgtcgat ttaagctcag ggagttcaac aatacactga cggaactaaa cgccatcgct
     9901 cctcctgcaa tgattggatt caaaaagcct agtgcggcaa aaggaatacc aatcgtatta
     9961 tagacaaaag cccagaaaag attttgtttg atttttctca atgtcaacct agacaactcg
    10021 attgtttgtg caatggatgc caaactgcta ttcatcagcg tcacatcggc tgtttccatc
    10081 gcaatgtccg ttccacttcc cattgccata ccgacatcag ctaatgctaa ggccggtgca
    10141 tcattgattc catcgcctgc cataccgact tttttgccat ctttctgaag tttttctacg
    10201 taggctgctt tatcctctgg aagtacttca gcaaagatat gctttggatc aataccaact
    10261 tgtttaccaa tcgtttcggc tgccagtttg ttgtctcctg tcagcataaa aacatctaat
    10321 cctttattct ggagctgctt gattgcttgt ttggcttcta gtttgatttg atcagccacg
    10381 gcaatccacc cgatcacttg cttttcatca gctaaaaaca tgactgtttt tccctgctgt
    10441 tctaattcta acgcgtgttc ttggtattca tcaaatgata ggttcaattc aattaatcgt
    10501 ttacgtgtac ccgcaaaata gcgtactcca ttaatcgtac cgctgatccc agcacctggg
    10561 tgggctgtaa aatcaatcat atcgtatgtt gctgcttctt gcagttttcc gtattccacg
    10621 attgctttac ctagtggatg ttcagaagct tgttctaaag tataaaaaat agataggact
    10681 tcttttgaac cgactagatc tgtaactttg ggctttcctt ctgtaatcgt tccagtttta
    10741 tccaaaacaa tgctgtctaa gtgagcagct gcttctaatg cttctccgcc tttaattaaa
    10801 attccatttc gtgcaccaac acccgttcct accataatag ctgttggggt cgctaaacct
    10861 aatgcacatg gacacgcaat gactaataca gagacactat ggatcaatgc gagttgccaa
    10921 tcaccagtga tcaaacctgt agctatgagc gtgataaacg cgatgactaa tacgatcgga
    10981 acaaaaatcc ctgagatccg atctgcgatt ttttggattg gggctttgct cccttgagca
    11041 tcttccacca tttgaatgat ttgcgctaat actgtctgct tgccgacttg tgtgacttcc
    11101 atgtgtagca gaccattcgt attgattgtc ccgccaaaaa gcgtgtcatc cggattcttt
    11161 tctacaggaa gattttcacc agtaagcata ctttcatcta tcgcactgct tcctgaaata
    11221 attcgaccat ctgctggaat ctgctcacca ggacggatga ctagttgatc accaacgact
    11281 acttcttcaa tcgctaacgt ttcttctttc ccgtttctta tcacttgggc ggtttttgtt
    11341 tgaagagaca tcaattgttt gattgcatta cctgttttcg tttttgctgt atgttccaaa
    11401 tatttgccga gcaaaatcag cgtgatgatc atactgctgc tttcaaaata aagatcttgg
    11461 gggtgtccac ggaaaaatcc gttgtaaatg ctcaatgcaa aggcagccga tgttccaatt
    11521 gctaccaata catccatatt tggtgctttc gtcttgattg catggtacgc acctttataa
    11581 aaacgagcac cgacataaaa ttggacagga gcagacagca ctaactggac gattggaaaa
    11641 tgaaaaaagt gaacaatcgc cgcatggctt cctaacatca tagcaatcat tgacaacatc
    11701 aacggcaaag tcaaaaccgt actcaatata agatcgaata gcattttttt caaatacgct
    11761 tgcttttctt ctgcaatttt ttgtttgtgg gcttcatcgt acaaaattgc accataaccg
    11821 atgttttcta cgctttggat taatttttca gtggtcgtcc cttcatattt gacacttgct
    11881 ttttcagtgg ccaagttgac tgtggcattg accacaccag gttgtccgtt cagctctttt
    11941 tcgatcctag cagaacaatt tgcacaagtc atacctgtaa tgacgaacgt atccattttg
    12001 gtttctgctg ttgccatcaa atcacctctg ctttataacc caatccattg attgcttgac
    12061 aaatcttttc ggattgaact tttgtttcat caaacttgac cgtaccgttt gcttttttca
    12121 aatttacttt cactttttgc acgccgtcaa gtgcgcttac tgtttcttct actcttgctg
    12181 cacaatgatt gcagctcata ccttcgattg aaaattgttg tttcattttt tcttcctcct
    12241 attgatgttg tttacattca cattgacctg ggatacagtt acattcgatc gtatctactg
    12301 gttgcttttg ttccagttgt ttcaaaatct cattgatatc ttcttgagtc aaagtggctt
    12361 cttctactag atccgcaatc gtctctccaa tcctttttgc acagatatgg gaaaaaagat
    12421 tttccgtcgc actgcgaact gtttccatct ccgagacagc tggatgataa atgaattttt
    12481 ttccttcttg ttctgtccag atcacttctt tttttaccaa acgtcccagc aaagttttga
    12541 tcgttgctgg tttccaatcc atcgaatcag ctaagatctg cgtgatttct tgggctgtcg
    12601 tctgtccaag tgtccaaata acacgcatga tctcccactc tgaatcactg atctttactg
    12661 gcgttttgac tttttccatt tatttcacac ctttcgttta caaatgtaat cattagctta
    12721 tgaatacaga ttacgcttgt aaacaaaaac tgtcaattat ttttgctctc tattttcaat
    12781 ttagttaaaa attcttgttt ctctcgtctt gttttaagga tgatcagttt atttcttgca
    12841 ttagctactt ggatcagctg ctgtattttc ggttcattgt tttcttcaaa atgccagata
    12901 aaagataaaa attctaagta ttccctgttt agtttctctg aaaatgattc aggcatatct
    12961 ggccgagatt ttttgtttat ctttcttttt agagaacgcc aaactactcg gtagatggcg
    13021 agataacgcg gcactttaag ccaaatgatc ttatctgctt ctttaatcct ttcctctaat
    13081 gtagaagtat aatttccttc aacaatccat gacggattag ataccataaa cgattgctgt
    13141 tcttgcaaaa accacttttt tgcctgaata ctgtagtctg ttgtatgcca taaaaggtct
    13201 agcggcagta gcggaaagtc ggtgatttct ttgatcttcc ttgcgagggt cgattttccc
    13261 gacccacttt gtccaatcac gatgatcttc attcttctcc ctcacttctc tttggaattt
    13321 ggttcgatca taccacttat aacgccgcaa aaaaacaagg gactgtgaca aaagtcgtgt
    13381 caaagtccct tgtttcgaat aaatggtgtt caatcagctg accttcagca ttaactcaaa
    13441 aaacggcaaa atatgaggag ctattttttc gtttgttcct ctaatactca ggtcaaatcg
    13501 ctgattttac aacctcttcc ttaaacggtg ttctcctgcc aacacctcgg tcacaaggca
    13561 caatagtcaa caaacatgag aagttgtttt ttgaaattgc tccttgtgac tcggagttaa
    13621 acggcaggct cactacctct ttcttaaacg gtgttcaaaa gcttgcatct gcggtaataa
    13681 gcccagcaaa acgaaaaata tgaggggcta tttttgtttg gctgttctta ttacttgatg
    13741 caggacagct ttttcacaac ctctgattca cggtgttcca gaagtagctt ctgcgaaaaa
    13801 caagccgaaa cttcacaaaa atttgagaag caattttcgt aaattccttc ttatttcttg
    13861 aagctgacca cttctgtcac aacctcttgt aacaagtatt tctattttaa ggctgaacca
    13921 ctgtttctgg ttcagtgatt tctttgtttt tgtctcgacg agcaattgct ggcaagatca
    13981 tacctaataa gatcaagaca attggtgtga tgatattcgt gaataattgg aaccaccatg
    14041 ctttaggatc agcagtataa tctacttttg gcaccattcc taggatacat gcaaacgcag
    14101 tgaaggcaaa gcaccaagca ccaaagataa aaccgatttt tggattttta acaaatttat
    14161 attcagaatt gaatttctta taagctttat tcaacatcat atatgccaag aacacccata
    14221 ggtaacgcat tggcatgaca actgagttca agttcgtcaa ccattttact aattcattca
    14281 tatctttgat accaaagatc ggcaacaaaa taatgattcc tactagtata cctgtcagaa
    14341 gataaccatt caccaatgta cctttacgat tacgtttacg tagccagttt ggtacaaatt
    14401 ctggatccgc atctgctaac aagattttta gtggtgcatc aattgaaaag gctaatgcgg
    14461 agatttgtcc aatcatgttt gtcaatgcgt aaatgatcat caatgagtta ccgataccat
    14521 agtaattacc taaaatttga aaagcactgt atgccccatt tgccatcaag tctttaggaa
    14581 tattatcgct agcaaacagc atacccattg cgactgagcc taatacagca caaatcccta
    14641 ccattgctgc taagaagatc atccctttag gaaattcctt tgcaggattt cttgtagagt
    14701 tgacataagg ggatattttt tccgctcctc caactgcaaa tactagcatc gaaacggttg
    14761 tgaaataact gaaatcaaat gttggaatat atgttttcac ttgattcata tgtggtgtgg
    14821 caaattccat cgttggattg atgaacggtg ccgttacagc taaaagaata aataagatcg
    14881 acatgacgaa catcgctgtt ccggccagtc cgccaatcac tttcaaagta gaaagtcctt
    14941 ttgttgataa atataaaaag gcaagaaata taataagtga aataactgct accatttgta
    15001 ctgacatcgt atttacgata tttccatttc cttggactgc ccaaccgcca gcaatcagga
    15061 ttgcttgagg cttttgtgcc aaataaggaa tatggacgac ccagtaagtc caagcggcat
    15121 agtatgccag tcgtttatta gatgtcgtat tttcgaccca tgaactgacg cctcctttac
    15181 tatccttgaa ggctgaccct aattgtccaa cgatcaatgc gtatggaata aaataaatgg
    15241 ccaaaatcaa tagccaagag gtgacaactg atactccttg ctgagcatag ttattaacta
    15301 cattccccat gccccaaacc atgttaaatg caattaacgc aacagtaaac cagcgtaatt
    15361 gtttgttgtt cattcgttct ctcctttatg ataaaaattt gttatccaga tgtaatcata
    15421 ttcaattttt aacaaactta caagacagtg ttattttatg tgtgtattct tttatttaat
    15481 acacatcgtt ttttcatatt ttttccatca aaaaaataag ttattttctt ttatttcatt
    15541 taccttattg aatttcaagc tattttgaat tttctgcaaa attaaaaaca ttagttattt
    15601 tataatccta tctattttct ttacattttt cattaagaaa aggatataaa aaaaagagga
    15661 cgttcattcg tcctcttcat tgatcaacgt ttatttttta catgcaagct tcgcatggca
    15721 ttcaaaacag ctaatactgt caccccaaca tctgcaaata ctgcatctcc cattgatgca
    15781 aaaccaaaag cacctaaggc aagtaccaaa atttttatcc caatagcaaa gacaatgttc
    15841 tgtttgacga ttttcaatgt tttctttgct aaatggattg cttctgcaat cttcgatggt
    15901 tgatcattca tgatgactac atctgctgct tcaattgcag catcgcttcc caatccaccc
    15961 attgcgattc ctacatctgc tcttgctagc accggtgcat cgttgattcc atctccgaca
    16021 aatgcggtct ttttattgtt tctttgtaag atttcctcta accgctgcac tttatcttct
    16081 ggaagcaatt cgctatatat cttgtccacg cctacttgct tcccgatatg atcagcaatt
    16141 tttttcgaat cgcccgtcaa catgactgtg ttcttcacac cagccttttt taattgaacc
    16201 aatgcgtcta cggcatcttc tttcaaggta tctgcaataa caagataacc actgtaactt
    16261 tgatcgatcg ctagataaag caatgtaccg atttcttgac ttgaaggaaa agagatttca
    16321 aatctttcca tcaatttgtg attacctgct aatacttgtt tgccatcaac ttcagctaag
    16381 atcccttcac ctgcaaattc ttggatatta gctgttggta aaagcggacc tgcgtagcct
    16441 tctaaaacag attgagcaat cggatgtgta gaaaactgtt ctgcactcgc aacatattgc
    16501 atgaatatct ttggatcgat ttttgtatcg atcgtctgga tcgaaaagtc acctttcgtt
    16561 aatgtgccgg ttttatcgaa taccactgtc tcagtttgtg caagcaactc caagtaattt
    16621 gaacctttta ctaatacacc gattttactt gctcctccga taccgccaaa gaaactaagg
    16681 ggtacggaga tcactaatgc acatggacat gagatcacta aaaatgtcaa tgctcgatag
    16741 atccattcag aaaacgccgc tccagaaaca acaagtggag gtaaaacagc taataaaacg
    16801 gccaatccta caacgactgg tgtgtaatag cgcgcgaatt tagtaatgaa attttcagct
    16861 ggtgcttttt tactgctggc attttctact aattctaata atttgctgat agtagaattt
    16921 tcataagatt tttctacttg aactaaaaat ggtttatttt tattgataaa tccacttaat
    16981 actgtttcac caacatatac agatttaggg actgattctc ctgtaagtgc tgacgtatcg
    17041 actaatgatt ctccttcgat gatcgttcca tctaacggta cacgttcacc aggcttcaca
    17101 agtacaaact gaccgatctg cacgtcttca gggttcacgg tcatcaatcc ttccgtcgtc
    17161 tgcacgtgag ctgtttctgg acggatcgcc atcaattcgc gaatcgattt tcttgaatga
    17221 ctgactgcca atccttggaa atactcgccg atttgataaa atagcatcac tgctacggct
    17281 tctggatatt caccgatcaa catagcgcca atcgtagcaa cagacataag aaaattctca
    17341 tcaaagattt ttcctttgac gatattcatc actgccttct tcactacatc atatcctatc
    17401 agcagataaa tcaataaata ccctatcaag cgaattggtt cattaaaatc aaataatagc
    17461 aataaagcga tacctgcaag ggaaaataaa atccggaaca gcgtatttct ctctttttca
    17521 tttccatgtt cagatacttt ttccttatct atttctgtta ctttgacatc tggttctaat
    17581 tccttcacta ctttttttat ttcttgttca atcgtagcca aatgatcttc ctctgcgtca
    17641 atcgttagtt ttccagaagt gaagtttacc gttgcttcct tgactccatt gatcttctga
    17701 actccttttt cgattttcat tgcacagttc gcacaatcta aaccttctaa tctgtaactt
    17761 ttcattttaa tgacctcctc gcctcaacat atgaatatct cttcataact aattataaat
    17821 gaacggttat tcatatgtca aggtatccga aagaaaaaat gaaaaacatc tgcatcggaa
    17881 aatttcttcc atacagatgt tttggcttat tctttctttt tcttttcgat cattgccact
    17941 gctcttatcc aacccgcact tgccttcttg atcttgacag gatagcaact aagtttgaag
    18001 cctgttgctg gcaatttgtc taaattcatt aatttttcga taggacaata tcttttttca
    18061 atccctgcaa aatgtccttc ccagatattc gaagctccac tacgctttca aaaatataga
    18121 aagagttctt taatgaaaat tatttcaact ttccataaac agtaacccca tataaataaa
    18181 agacgtaaaa gtcacatgga agaacttata caggagaacg catagagtca ggtgaaatac
    18241 atgtatccca cctgactcta tgctttatct aacctttatt attctctttt atacaatttc
    18301 tgcaccgaat ggcatcaacg ccaatcgagc aagtttgaaa gattgcttgg caaaaggtat
    18361 ccctatgatc gtgatacata gaaggatgcc gctgacgaca tgtgcgagag caattggtag
    18421 gcctgaaacg atcagccaga tgatattaac aatcaatgac atgccagact ggtgataaat
    18481 tacttctttg ccaaaaggcg caaaagaaag ggaggcgatc ttaaaacatt gcataccgat
    18541 tgggatacct atgatcgtga tacaccaaag tatccccgct gctaaccagc taagaccacc
    18601 gattagtccg ccgaatataa accagatgac atttcctaaa caactcattg tatttctcct
    18661 tctattactg tttttgatta aaactgggca aaaaatgatt ttattcttct ttttttagct
    18721 tccagtatca aatattctgc aaattttttt tggatttctt ccgtattttc ttttgttact
    18781 aatgtgtgtt ggattagttt tcctgatggt cgctgatcca atcgttcttc tgactgttgc
    18841 acgtccgctt gcgcaaatcc gataatccgt ttttcttttg ttacagcaac gagcaatagt
    18901 ttattcttat catttaggtc agctgccgtt tttgctttta cgaatgattc tggatgatca
    18961 gtaatcagct gattgatttg aggtagatct tcttttactg cccaccgtat ttccatcatt
    19021 tctcactttt ctttcgctca tttttatctc cactaaaaaa atcgactgcg gcaattgcat
    19081 ctatccagct ttgtgcaatg atctcatgtc cgccaatcgt tgggtggacc ccatcttctc
    19141 ttgtatattt cgaaaaacca taaacaatcc ctaaagcatt gaatatacca tctaaaggaa
    19201 ccagcaatgc gccatattcc ttagcaagtt tacgaacaat ttggatttta gggtctaagt
    19261 ctttacgcca gccatgacgg tcgtcggtta taggaagtac aaacggctcc atcaagatga
    19321 tgttgcagat tcccactgtt tgaagttcat ttagtaattt tcgataaacg gattcaaatt
    19381 gctgagcaga tttctcagtt ccgaatatag cctgatcgct agtattatgc caggtatcat
    19441 tgattccgat caaaatcgat acatagtcag gttgtaaagc taaacaatct tcttgccagc
    19501 gtacttgcag atctgcaatt ttgtctcctc ctatcccacg gttcatgcat tcaaattcca
    19561 tcgttggata ttgggagatt agttttccgg caatcaaaag tggataacct ttacctagat
    19621 cagttgcttc tgctcggtct cttcctgcgt ctgtaatgct atcaccgata aataatatac
    19681 gttttttcat ttgtcaaaaa ctcctttttg aataaaacga tgattttctt ccttatatta
    19741 tcccataagc cctatcaaga cgaaagagaa aatatatgta taaaaaatga caccagccta
    19801 gtcccttcaa acaattgaat gaacgctcat caagtgtcta gttttttatg ctattttttt
    19861 cttaacgtgc cggattcccc ttgattcctt cgttgtttgg tccagagaga tcgatcacac
    19921 ctaagcttcc gtcaatcaga ccactgatga cgccatcaga acatatattt ataggctcat
    19981 ctaagacttc tagtaaatgt ccctttacta atatctcttc tagattgctt gggatagatt
    20041 ggtcataact ttatctggtc cgatgatttc aagggtgaat tgaacttaca tatggattat
    20101 tgcatgctga aaatacggac ctactccatc ttcgaatgtt actattagtt tgttttcagg
    20161 tgctagatgc tgctttattt caacagcaga agcatcatca aatcagattt ccatttgaat
    20221 taccttcttt gtaaatgttt gattgattgc gatttcgtta tagaaccttg tgaatcaaat
    20281 agcaatttct tttgctttct tctcttgtct gctggaaact tcctttatcc cgtattttaa
    20341 aaaaagaact gcgagagttc gcagttcttt tttagaataa acggtgctct ccggccaact
    20401 cctcggtcac aagtcacaat agtcatcaaa catgaaaaac tgttttttga aattactcct
    20461 tgtgactcgg agctgaacgg caggcgcaca acctcttcct taaacggtgt tcaaaagcca
    20521 gtttctcggt attaagccaa atttatgcaa aaatgtggaa agccatttcc acaaaatttt
    20581 tcttaatact cgaaactaaa cagctttttc acaacctctc cactaaacgg tattcaaaag
    20641 cttacatctg cggtaataag tcccgccaat tcaaaaacat gaacagctgt ttttgcttgg
    20701 ctgttctaat tacttgatgc agaacagctt tttcatcacc tcttatggat aaattcggtt
    20761 caacaaacgt gggaatggaa tagcttcacg tacatggtcg ataccagcaa tccaagtgac
    20821 tgttcgttca agacctagac caaaaccaga gtgcggaaca gaaccgtatt tacgtagatc
    20881 aagataccat gaatattctt tttcatccaa accgtgatta cggatttgtt ctagcaagaa
    20941 atcatagtct gttgcccgtt cgcttccgcc aataatttca ccgtaacctt ctggtgcgat
    21001 catatctgca cagatcacca cgtcttctct tgttggatgt ggtttcatgt agaatggctt
    21061 gatggctttt ggatagttta ggataaagac aggctgttcg aatgaattcg caatgaacgt
    21121 ttcatgaggt gaaccaaagt cttcgcccca ttcgatgtca tcaaatccat ttttcttcag
    21181 taattcgatc gcatcatcat aagtaatacg agggaatggc aattttgtat aagtttttaa
    21241 gacttctttg tctcgtccta gtacatctaa tgcatattcg caatgatcta aaacattttg
    21301 tactaagaat gctacatatt gttcctgtac ttctaaactt tcttcttgat gagtgaaagc
    21361 catttctggt tcgatcatcc agaattcgat caaatgacgt cgtgtttttg atttttcagc
    21421 acggaaagtc ggaccgaatg aaaagacttt tccgaaagcc atagcagcag cttccatgta
    21481 taattggcca ctttgagata aataagcttt ttgatcaaag taatttgttt cgaatagatc
    21541 cgtagtgcct tctggtgcag aaccagtcaa gatcggcgga tcaattttga caaaatgatg
    21601 ttgattaaag aattcatacg ttgcacgaat gatttcattt ctgatctgca taatcgcatg
    21661 ttgtcttgaa gaacgcagcc ataaatgacg atgatccatc aagaagtctg ttccgtgttc
    21721 ttttggtgtg atcggataat cgtggctctc gcttaccact tccacatttg taattccgat
    21781 ttcataacca aattttgaac gactgtcttc acggatttct ccagttacta acacggatgt
    21841 ttcttggtgt agatttttag ctgtttggaa aacttcttcc gacacttcgc tttttacaac
    21901 tacaccttgg aaaaaggctg ttccgtctct taattgtaaa aatgcgattt ttccgcttga
    21961 tcgcttattt gcgacccaag cgccgattgt tactgtttct cccacatggt ttttcgaatc
    22021 gatgatttga atttgttcca cgtatgactc tccttttata ttccttgaat ttctttagga
    22081 ttaattttga cttttactct caataaattg cttgattctg cgtaccgctt cttctaacgt
    22141 agcgcgatca gtagcataac tcatgcggac attttctggt gccccaaagc cttcgccagt
    22201 cacaagcgct acccctgttt cttctaaaag atcttctacc cattgtgtga cattttcata
    22261 tttacacatg cgtagcgttt cttttacatt aggaaaaaga taaaaagctc cttgtggctt
    22321 ttcaagagaa acacccggca gttctgccac aagcgaatat aacgtattca ggcgttcttc
    22381 aaaggcgttc cgcatttctt ccactgtatc ttgctcgcct ttcaatgctt caacagctgc
    22441 atattgactg actgctgtcg gattactggt tgactgggaa gcaacagcga tcattccatt
    22501 gatgatcaca ggatttccca ccgcataacc aatgcgccag cctgtcatcg catatgtttt
    22561 agacacgccg ttgatgatga tagtctgatt tttgattgct tctgaaatcg ttgcaattgg
    22621 agtaaactca ttgccattat aaactaaacg accataaata tcatcagcaa ctatcaaaat
    22681 atcatttttt actgcccatt caccgattgc ctgcagttca ttcttgctat aaatcattcc
    22741 agtcggattc gatggagaat tgatgattac ggcttttgtt ttttctgaac gtgcttgttc
    22801 tagctgttca acagtaactt tccagctatt ggattcttcc cctttcacaa agacaggaag
    22861 tccttctgcc agttttactt gctcaccgta actgacccag taaggcacag gaatgatgac
    22921 ttcatcttgc gggtcaagga tcgtttggaa aagggtatac aaagcaaatt tcgctccgtc
    22981 tgttacgact gtttgtgaag gttcatagac aagtccatag tttttcttca gataatcaac
    23041 gattgcctgt cgaagctctg ggattcctgc tgttggtgta taatagctag ctttcccgtt
    23101 gcggattgct tcgattgctg cttcttggat gttttctggt gtcgcaaaat ccggttctcc
    23161 cacagtcaaa ctcagcacat ctctgccttt tgcttttaat gccttggcct ttgccgcagc
    23221 tgccaaagtg acagacggtt ctaatcgttt tgttcttttt gataactcca tcccattcat
    23281 tcctttacct tttaaattcc cttgattgtc ttgacttcat ctccagattc aaagccaatc
    23341 aggtaataat tcagtttccc atttgtttct tttcctacta cttcccacac aggctgatca
    23401 tcatacatcc ctaaagcagc tttttcgatt tggatttcag gatgggccgc agcaattgtc
    23461 tgttttgctt cgttttcagt cagtcccttt gtttgatcca atacttttac tttttcacca
    23521 gatttaggaa tgataacggc gatttctttt cctttatcat ttgtacctgt cagtgaaaag
    23581 tatgtcttct ttcgagtaaa ccaataaaaa tgatcggctt cctcaagatt tgcatatttc
    23641 tttgcgattt ctactgcttc tttcttcgcc tgcaccgccg gacgactgga acgcaagtaa
    23701 aaaatcgagc tgatcacgat gaccgctaat aaaaatacga tcaagccaag taacacgcga
    23761 ttaatcgttt tttcctttgt ctgttttttc ttcaagggtc gtcgctcctt tatcttcatt
    23821 ccttttcatt atagcagatt ttaattatga aaaaagacag ctcctctaac ttccttcttc
    23881 atttttcttt aagaattcat ggatgtcctg atagatttcc tccatagcta gctctttgat
    23941 cggtactttt ttcggtaatg catggatgat ccgtgcacca tattttgcag tgactagtct
    24001 tcgatccaaa ataagcaata cgccacgatc tgtctccgtc cttatcaagc gacctaatgc
    24061 ttggcgtaac ttcaaagctg ctttaggtac ggattcctgg taaaaaggat tgatcccctc
    24121 tgattctaaa taagcgttcc tagctttgac catcgggcga agcggattat cgaagggtaa
    24181 acgtgtgact acaacgatct gcaaagcatc cccaggcaaa tctacacctt cccaaaagct
    24241 gtcagcgcca aacaatacgc ttttttctga caaaatgaag cgtttcaaaa gcttctcccg
    24301 gcttccgcca aacccttgag ctagtatttc tcgtcctttt tctagcatag acagatgcat
    24361 tttctgatag accttctgca aaacatcatg agaagtgaat aacacaagta ttggccgatc
    24421 ttcttgttcg atcagacctg taagtgctgt ctctaaataa tcgatatatg cttgattgct
    24481 gacttgatgg acggcaggtg cctcttttgg cacatagatc cttgcctgtt tctcataatc
    24541 ataaggagag gcaatcactt taagaggtgc tgcttcaatg ccaagccgct tagagagata
    24601 gtgtcgatta gggccgatct tcagcgttcc tccaatataa agaatacgat catatcgagg
    24661 ataccaaaca gtcgacggca acatagccgc ttgaaaatca tggagatgaa aaatcatctg
    24721 ctgcgctgtg ttcccaggaa cgaaccaatg gatcaaatga tctcgccagt cttccagcca
    24781 ttgatccatc agcaagtact gcttgtctaa ttgatcaaat aagctatata aacttgctaa
    24841 atcgatccgc atctggtttg tccagctttc ttggataatc tcaaaagtca gacgcatttg
    24901 tgactgtagc tgctggatct cctgataaaa aagctgcagg tgatcaatgg ctgtcccaga
    24961 ttccagtggc agctgatcca acatctcttt tgtcatcatg acttcttctg gatactgtcc
    25021 gttaggttgc ttgccgctca ctaaactcat taatccttct gccatacaaa tctgtgcttc
    25081 gcatagcgca gaaagttctt ctcgataaat cgataaccag cgtaaggctt catgttcttt
    25141 cggtaaatag ctttgtattc ggtcaaacag ctgatttggt tcttctaatt gctggatttg
    25201 tttatggaaa tagttaatac ttaatcgttt gtcatttact ttttcagcga tttccggcaa
    25261 atgatgtact tcatcgatca acaaaaatgg actagctgga agcaatggtt cttttcgctg
    25321 tgtttcttgt attaaaaacg catgattgac aatcaaaaaa ttactttgcc tgattttctt
    25381 ttgcaagaaa cgaacaaagt cttgttcgta aaatgactga tttcttgcta aaaatccaat
    25441 acctctgtgc gcgatatctg caaagaatga atggttcaaa tttgttaaat gtaattcatc
    25501 caaatcaccg gtcttcgttt gcgtcagcca aactaagacc gccatttgat acaaagcata
    25561 ttgtttttgt tctggcggtt ccacaagtgt gcctttaaaa cgctgtaaat caatatagtg
    25621 gcgatagctt ttgataatta caggttgtat gggctgatct agcaattgat tcagcaatgg
    25681 aatgtctttt tctatgatct gctgctgcaa aactagcgaa acggtactga tgacagcggg
    25741 tttttcaggt gtagccaaat aactcaatgg cagcagataa ccgattgttt tacccattcc
    25801 tgtagctgct tcgatcatca aatcttttga ttcatctttt gtgaaatggt cgtagaccgc
    25861 attcatcagt ctgttctgtt cttttcgata catcagtgtt ttaccaaaca ttttttcttt
    25921 tgcttgtttt tttcttggat atgtccgctc gccataatgg acggatgtga atagttccac
    25981 ttcttttttc tgcaaagcta gcccatcgac gatttctaat gagggatcaa gtggctctgg
    26041 tttttctttc atttcttcaa tcacatgata aatgaaacga ctcgtatcca tacctgtgtg
    26101 tctacttaat cgcgcaatct gctctaacgt gatgattggc aattttctca tccgttcttc
    26161 gatcagcaaa agaagttgac ccgtcacttc ggcatcacta tctgcctgat gaggattctc
    26221 atgaataaat ccaaggcttt ctgacaaatc agctaagcga aagcttggtt ccgtcggcaa
    26281 gaaaatttgt gcaagttcta ctgtatcgat gcctggtatc ttcaaactag gaagtccgca
    26341 tcgcatgagt tcatggttca aaaaattata atcaaaataa atattatgcg cgacaaatat
    26401 ggtgtctgcc aaaagattgt aaatcgtttg ggcgacatct tcgaaataag gcgctttttg
    26461 gacgcgttga ttcgtgatcc ctgttaaatg ctggatttgt tttgaaatct tccgccctgg
    26521 atgaatatcg atcgaaaaac gtgaagtgat ccgacctgat tcaatcatga cgcagccaaa
    26581 ttggatgatc cgatcttctt ttggattcgt cccagtcgtt tcgatatcaa cgactgcata
    26641 agttcttggt ttcaaaatat cttcctcttc tcctgcctct aatgttcttt tgaaattata
    26701 ctacaaagtg cagaaaaata
//
LOCUS       DACGLW010000032        26538 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.032, whole genome shotgun sequence.
ACCESSION   DACGLW010000032 DACGLW010000000
VERSION     DACGLW010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26538
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.032"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            complement(99..1229)
                     /locus_tag="IXT53_001382"
     CDS             complement(99..1229)
                     /locus_tag="IXT53_001382"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HAQ4905217.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     gene            complement(1878..3080)
                     /locus_tag="IXT53_001383"
     CDS             complement(1878..3080)
                     /locus_tag="IXT53_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ4905218.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3140..3343)
                     /locus_tag="IXT53_001384"
     CDS             complement(3140..3343)
                     /locus_tag="IXT53_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HAQ4905219.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3485..4717)
                     /locus_tag="IXT53_001385"
                     /pseudo
     CDS             complement(3485..4717)
                     /locus_tag="IXT53_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
     gene            4880..5692
                     /locus_tag="IXT53_001386"
     CDS             4880..5692
                     /locus_tag="IXT53_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ4905220.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6716..6868)
                     /locus_tag="IXT53_001387"
     CDS             complement(6716..6868)
                     /locus_tag="IXT53_001387"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905221.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
     gene            complement(7059..7937)
                     /locus_tag="IXT53_001388"
     CDS             complement(7059..7937)
                     /locus_tag="IXT53_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ4905222.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(7976..9094)
                     /locus_tag="IXT53_001389"
     CDS             complement(7976..9094)
                     /locus_tag="IXT53_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HAQ4905223.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(9091..11190)
                     /locus_tag="IXT53_001390"
     CDS             complement(9091..11190)
                     /locus_tag="IXT53_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HAQ4905224.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(11241..12062)
                     /locus_tag="IXT53_001391"
     CDS             complement(11241..12062)
                     /locus_tag="IXT53_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ4905225.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(12059..12868)
                     /locus_tag="IXT53_001392"
     CDS             complement(12059..12868)
                     /locus_tag="IXT53_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ4905226.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(12889..13383)
                     /locus_tag="IXT53_001393"
     CDS             complement(12889..13383)
                     /locus_tag="IXT53_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ4905227.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(13395..13883)
                     /locus_tag="IXT53_001394"
     CDS             complement(13395..13883)
                     /locus_tag="IXT53_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ4905228.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            complement(13960..16656)
                     /locus_tag="IXT53_001395"
     CDS             complement(13960..16656)
                     /locus_tag="IXT53_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ4905229.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            complement(17084..18157)
                     /locus_tag="IXT53_001396"
     CDS             complement(17084..18157)
                     /locus_tag="IXT53_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ4905230.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            complement(18154..18981)
                     /locus_tag="IXT53_001397"
     CDS             complement(18154..18981)
                     /locus_tag="IXT53_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ4905231.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            complement(18982..19788)
                     /locus_tag="IXT53_001398"
     CDS             complement(18982..19788)
                     /locus_tag="IXT53_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ4905232.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            complement(19800..20885)
                     /locus_tag="IXT53_001399"
     CDS             complement(19800..20885)
                     /locus_tag="IXT53_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4905233.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            complement(20902..21444)
                     /locus_tag="IXT53_001400"
     CDS             complement(20902..21444)
                     /locus_tag="IXT53_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ4905234.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            21702..22691
                     /locus_tag="IXT53_001401"
     CDS             21702..22691
                     /locus_tag="IXT53_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HAQ4905235.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            22688..23425
                     /locus_tag="IXT53_001402"
     CDS             22688..23425
                     /locus_tag="IXT53_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HAQ4905236.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            23422..23973
                     /gene="coaC"
                     /locus_tag="IXT53_001403"
     CDS             23422..23973
                     /gene="coaC"
                     /locus_tag="IXT53_001403"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HAQ4905237.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            23989..24621
                     /locus_tag="IXT53_001404"
     CDS             23989..24621
                     /locus_tag="IXT53_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ4905238.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            24706..25353
                     /locus_tag="IXT53_001405"
     CDS             24706..25353
                     /locus_tag="IXT53_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ4905239.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            25358..26341
                     /locus_tag="IXT53_001406"
     CDS             25358..26341
                     /locus_tag="IXT53_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HAQ4905240.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
BASE COUNT         8114 a         5605 c         4500 g         8319 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaaatcct tttactagca aatactagta aaaggacgat tatactcgtg gtaccacctt
     1321 ttttcagaaa aaacattctt tctctcagcc tgacaataaa atcagtggtc ttggtaacga
     1381 aagactgttc gtttttgcct acctttcgac aaaaaatcct ctaaggccat tttcgacagt
     1441 caacttctag ctttttacac caaccaaagc ctctcttgaa ccattttctg tctactttcc
     1501 ttctcatcgg atactattag aataaaatta gaatagcatg tttgaaagaa aagtcaatag
     1561 ggaataagaa caaacaaaaa ctaatttgtt attcttagta atttgtttgt ggaatacaat
     1621 aaagcttttc taatatgata attgttcagt cgtcctttca tcttagatca cttccttaaa
     1681 atgatcatac ccgtttcaac cacataaaaa aggacttaat tttgaaaaat ttcccatcaa
     1741 aactaagtcc tttttattat agttatgcaa atttgcttat tttctcgcta tttccgatac
     1801 atcttgaaca ggagcaattc gactgaaaac actggtgcaa aagcatttat ttgtcttatg
     1861 gtagaaccgt ggaaaactca gactggtaat aaattgtcat tttttgaaag aatagcgccc
     1921 tcttgaacat tatcaatcgc acttaccact tgttgatctg actttgcata taagtgaccg
     1981 taagttccta aagtgatttg aatattcgca tggcccaatc gttcttgaat agatagcggt
     2041 tgaatattct gctcaattag aaatgcaaca tgagaatgtc gtagcgcgtg aagcttaatc
     2101 ggtttgacac ccgctttttt agcagcttct tttaaccagt tcgcaaaaga ataaggtggc
     2161 gtaaatgttc cgtccaactg agcaacatat tcaaaatttc caatacgttt ttgcaagtca
     2221 taccactgtc tgagttttaa ggaaagcttc ttccccaacc caatcgttcg ataagcacat
     2281 ttagttttgg gagtcgaaaa gtaccaatca tcttttgtat tataaatcaa tgtcttctca
     2341 atcgttatcg tgtttttctc aaaatctaca ttcgaccaag acaaagcttc aagttctcca
     2401 atccgaatgc cagtataaaa gagcatttca tacacaatct tccgatggtg ggtttgaatc
     2461 ttatcatcct tcaattcatt catcactttt tgaaactcac tgtgttccca aaactcaact
     2521 ttcttattct cagaaggaag cgaagaaaca tcttcaacgg gatttttagc aatcaccccc
     2581 tctttaacag cccgatccaa tacaattttt aggtgccctc gaatccgatt gatataagat
     2641 tttgataacg tttcgccatt aacttttttc cgtgaattac gtgactctgt ctggcttagt
     2701 tcaaataacc aatcctcaat atcgtgactt gtaatatctc gaacacgaaa atgatagaaa
     2761 aactgcatac gcttttcgaa aatgaattgc gcattctcat atgtttgact tttgacctgt
     2821 ctctcgtacc attttacaaa cctatccttg taaaactctt cgaaagtcat ttgattggta
     2881 gaacacagtt gattaggaat tgtcttgtta aaatcatgtg tttccatgta cttgtcataa
     2941 gcaattttgg catctttttg tgaagcataa cctcgtttag tcttctgaac ctttcttcca
     3001 ttttcatcta tacctagata cgctcggaag aaccattttt ttgtcttttt atcttggtaa
     3061 atatttttaa tttttggcat cgttccattc ctccctgtac ttgagaaaag ctactttaat
     3121 agtaccgcgc ttgtactgtt tattccgcaa cttgaatgtg aaaaagatgt tcaattactc
     3181 gggatggcac cacaccaact tggcgattat tataaaaatc aataccttca atccttgcaa
     3241 gatatgcctt gctctcttta atcattcgtg cagcttgact aggtttaaag cccatctcta
     3301 tcaaaattgc acgatttacc atgaaatcac attttgtttc cattctctca cctcctgatt
     3361 ctttactcct tttaaaataa tcctctttat aatctattgt ttctcattcg atggttgtgt
     3421 tatactcgtc ttgtcgaggc ggagtacgtt gcacaacttg ttgtgtaagc agtactttgc
     3481 ttttttagta gcttgttttc tcatttgata tactttgtaa ttttcgttca taatactcaa
     3541 cctcctcctg ataatactcc gggtctgcct tcatattttg gagaagtttg atctgtcttg
     3601 gattcatttc tccttgttca ataatcaaag gcaacaagga agaatgaaac gtttgatcga
     3661 cctgtgcaat aaaaactaat gatggtgaaa caccatgaat taaccaactc atagttttct
     3721 caaaactttg aggaattgta tctaaagaaa tggtgattgc gccataattg ctaataaagg
     3781 ctgcccattt ggcatcaact tttgcaccta gatcatttgg atcacggtca taaaaacaaa
     3841 ttgctccatt aagtaactca aagaacagtt tggttaaatc atgggttttt gaaataatct
     3901 ttgccaagct ttgcgctttc tcttttcgat accgtagttc aaagcgattc tttacttttg
     3961 cctcttctaa aggaattcct cgtcgcttcc gttgttcatg gtccttctga taaaagcaaa
     4021 aacgacactg actcttcttt gagcctaaat acaaggtctc accatttgat tcattgttta
     4081 tgatatccgt accgccatga gattcactca cttgaaaagt ggtccacaca tggcctagta
     4141 ttaccttatg cttaagttct ggaatgctga gactccccac aaaatcatct aaagtaaaat
     4201 caatccgtgt aaagttcccc tgataatcta ataccttacg aaaaaactgt tgccaatttg
     4261 tttttcgtgt ttttagccat cttgctaagt gtttacagcc ttgacctgaa aactccatca
     4321 cagttccttt aaccgcatcg tcaatggaat accgaatcgt gattacttga ttccacacgt
     4381 attggccaat atagcccttt ggcgcataat ccagttcctc aaaacgttca atgggcattt
     4441 caataatctc ttcaattagc tttaaatgtt gattcgagaa aaagtgaatg gttagataat
     4501 caatcaaaac acggttaact ggaacgacct cttcttgcaa actatccaac acaagattca
     4561 atttttcttc acttaattgt ctcgttcctt tttctacttt cgataataac tatctcgaaa
     4621 tccccacttt ttctgctaaa tatcgttgtg tccaccccct ttcttctcga gaaactcgaa
     4681 tggcttcacc gagatcatgt tgattaactt ggttcatcga caaacctcct tctgctattt
     4741 acttgtcaaa gaacctattg aacttatttt aagtacattt ttattgtacg cctgtattta
     4801 ttttaagtca atatgttttg acttttttta agtacacacg tatactaaat ttaagaactt
     4861 atcctgagga ggagttttca tggagataga caagcaagca gtcggcaatc gaattcggca
     4921 gattcgccaa gaattaaagt tgagtatgga gaaatttggc aaactaattg gtgatttacc
     4981 gcgaagtaca gtcaataact gggaacgtgg gatcaacctt ccaaaaaccg aaacccttca
     5041 ccaaatcgca gagataggtc atgtcaccaa tgaatatctt ttgtatggtg atcaagagaa
     5101 tcaatacatt ttagaaatgc tccaaaaaaa ggcaggtaag cttgatccta agattgaagg
     5161 tctcatagta gacgaaatca aacacgctga ccttgttagt gaaaaagaaa tggatcgaat
     5221 gattgaattt tttattacaa atctcattcc accaactgaa caagaccagt ttactttcca
     5281 atgtatcgat gaaaaaaagc agctttatct tggttcaact aattttggta aggaagccca
     5341 gctctatttg caacatgata atcaaaatca tattctgcac atgatgccct ttaccttctc
     5401 caactttagt gtggatcgca tactcgtcta tttagcaaat caaaaatcct attcctattt
     5461 tgggaaacat ttacccaagg aactaggtga gaaagccatc cttctatact caattaatca
     5521 attgaccagc gatgtgcgaa ttgccccgtt ggtctattca gaagaaaccg cttcttatca
     5581 gtacacagag gacaatcaat atctactaga acagcaatat ctttatctac cattcgtgat
     5641 ggaagtcgag aaagaacgcc tgttaaatgc agcgtatcca tcatcaaaat gaggtgacaa
     5701 aattgataag cacgtgacaa agaaaatact gctaggacac tccccaatgg gtagttgtca
     5761 cagtttcctg ttctttgtta gccccctgtt agaaccgggg gctttattcc tgcagtccgg
     5821 ctacgccttc ctgcaataga caaaactcca gatcgggtaa ttcgcttgtg gcgaaacccg
     5881 acctggagtt tgtctctgta ttttgaaccc taaaaattaa tcaaacagac tgatattctc
     5941 ataatcagcg cgaaataagc cattaagtac agttttccta cctatttttg ataacccgct
     6001 ggttagttaa tattcctttt ccgagagaga taaatgattg gttaaatcta tatacatctc
     6061 aacctttaaa aatgttttcg aaatacttca tcagtacatg tcatacaaat taccagtgca
     6121 ttctcaaatt ctagttcttt aattgatttc agttgaggag cagttacttt tggcggaaat
     6181 ttccattttt gtccaaacct cttcccccga tgaaatttct ttgttgcaat tattgcaatg
     6241 aaaaattttt gtttcaaaaa tatacataat tacttcctcc aataaaaact atctacctct
     6301 caaattattt ttctctaata agaaaaacag aaaaggtaat aagatacaac agtgatgatt
     6361 cctaataaag aaaagactac aataatattt ttctaactta tcgccttcaa agaaatagtt
     6421 tcttattttt cactaaaata aaaaatttat tgcaaaaaaa atagaagtga caaaattatc
     6481 aatcatttca aggtaatata actacctgag tttattatcg attaaaatgc taactacatt
     6541 tgcaagagaa gcgagagtta aaggtaaata tagaatttct acttttttag caatagaaat
     6601 aggaataaaa tatttaaaat gtatagaaac tttctttttt tttcttgcta tctttttata
     6661 atcacaactc tctataatag aattacgatt tattttatac ggctgaaaaa atctatcacc
     6721 aaaaaaattc aaacattaag ccaaggatta tgaagtcaac atttcgaaaa atgaacatac
     6781 attggtaagc aactataact attaccacag acataaagta acacaatata ttactattta
     6841 agtgtacgcc accagtgaat atcctcaagg tatacagcgc acaacaaaaa gaaaaaagga
     6901 aacgcagaag catttccttt cagtgattta atgaaaacaa cacgatttga cttagagcct
     6961 ttttatttga attcattttc gctttatcca acttcatttg atcatttgat tactcggtta
     7021 aatacttttg acatgagtat caatgaaaca ttcattaatt atggttgctt atttcttacc
     7081 gcttttctgg ccaatgctaa tccgccgatt attccggcat tatcacctaa ttcgggcgta
     7141 ataatgtagc tatcaattgg tggtatttca acatatccat ttataagctc aacaaattta
     7201 tcctgaactt ttttcttcat gtgttcttgt ttcattacac caccgccaaa aataattcta
     7261 tctggcgaaa acatcagtgt tgtattgtat gcacattggg caaggtaaaa ggcttcaatc
     7321 tgccaaaacg aatcgtcagc caataaattc tggcctttta cacccaaccg tttttcaatc
     7381 gctggtcctg ctgccatccc ctctaaacaa tcttggtgga agggacagtt tcctgaaaaa
     7441 ttgtcctttg gatgtcgctt gatcaacata tgccccattt ccggatgact gaacccttcc
     7501 acgaaagtcc cattgaacaa ggcaccacct ccgatacctg ttcccacagt gaagtatgca
     7561 acacctgcta accctttgcc agaaccagca acatattcac catagcaagc cgcattgaca
     7621 tctgttgtcc attcaatcgg aatattgaaa tattgcttta gcgttcctac aaaattgaaa
     7681 ttttgccaag caagttttgg tgttgatgtg atatgaccga aactcgttga tttgcattga
     7741 atatcaattg gaccaaagca gccgaccccg atgcctgcca attgttcctt aaaaggttta
     7801 aaaaaatcaa tgacctgctt cattgtttca actggagttg ttgtgggaaa actgactctt
     7861 tccaaaatcg ttaattcctc agtaccgact gcacagacaa atttagtccc tccagcttca
     7921 attgccccat atatcatcat aggttcctcc cttcgaatat gtgaaaaaca cttcattaac
     7981 gcggcaagcg ttctcgatca gtgaaatgac gtttagtatt tcctgaagct tctgttgaat
     8041 accagtaagc gacactagca acatcgtctt gccgctcaaa taagcgaaca tcatcgttgc
     8101 cgatttgttg taaagtgacc gataaatctt cggtaaaaca gatggggtct tgaagatgcc
     8161 aacgataaag accatgtcgt ggcattgcgt cgtcaccaaa ggcatgaacg gcattcaatt
     8221 ttcccgtttc aaaacgatct cttgtggcat ctctatttga ttggtaagga taccctaaaa
     8281 aggctgttga atagtttttc gcatgtggac gtccgaactc atcgtactca tgaaaggccc
     8341 acgccccgcc aaaataatct tctgaacctg ttgaggttac ggtcggatac tcactatccc
     8401 catcaatgta gaatttaaac tcgccttcgc cccaccaata acgttctagg caggtaagcg
     8461 caaggtaagt acccacgtaa tagccttgac cctttaatcg atcaattaac ttataatcct
     8521 cctgtaattt ggtaatgcgt tcccgatgcc aatttgcgtg aaaatatagg ctattttctt
     8581 ccaattcgtc caccaaagcg taatttatcg tataaaaaaa tgaggacata tccttcggat
     8641 gtaaattctc gatcgtaatt ttagcggatg tttggaaagg catttcgaaa taggaattaa
     8701 agccccctgt aggattaacc actatcggca acgagttcac ctcgcaacgc tctccaaacc
     8761 cattacaaaa gaaatcccct aaaggacttt caacagcagg gctatccgac ccatcccaat
     8821 aaattcttaa gattaaatca cgtaaaacaa aactgccaaa ttctgttcga tcacgaatcg
     8881 tcatccacat atgacgaatt attcccgttc cagtaatatc cgcaatcaca gtttcttcac
     8941 cagaagtcag cggaattgcg ccacgacctt tgcggcttgg tcctagctcg cttggcaaca
     9001 tcgcagcttt acctttctcg cctgttggat tttcgggagt gattgctcga ctctggattt
     9061 ttttaaattg ggtaatattt tttagtatac tcatcttttt ctcctttgaa tgtgaatcgt
     9121 ttttcccttt tctacgtgaa gaattttttt cgtatcactt attttcaatg gcttttctga
     9181 caaatttgaa atactcaact cggattgcgt acattcaatc gaaatgaagg cttgaccgaa
     9241 cttcaaattc ttccaagaaa acgttaaatt atcaacagga tcaatttgca atactccttt
     9301 tttgaatttc aatccgaaat attgactgat cattaggctg acaaaaagac ccgatgccca
     9361 gccgcatttg ccgcagccaa agtctcgcac gacttctcct tgttgagcct ctaagcgata
     9421 cggccaccac caccacgaac cgtctaaatc tgccaaacaa attagttctt ctaatcgggt
     9481 tacgaaagct tgagggttgt gcagctcgct cattaaaaca gtgataaagc caggaaaagt
     9541 ggcgccggat tctaatcccc aagtgatgcc tttgatttcc tcgctatagg ttggattgaa
     9601 agaactagca gaaaaggcca tggtgttctg atataaatga tctttattat ctaaaaattg
     9661 ataaaaaggc atcaacgtag tgtctgattc ttctccatca tgcatcaata aattaatttt
     9721 tccatccaca atcacatcac ttaaaaagat taacccttgc tcaagaatcg gtttttgata
     9781 gtgcttcact gaataggttg ttttctcctc atcaccgatt ccctccaaaa attgctcacc
     9841 aaaaggacca tcgatagtca tttgtttaca aattgattcc tttagtttac aggcaatgtt
     9901 tttgtatcgc ttgctaagac tcagttgttc tagggcttca aaaatttctg caagtccaat
     9961 acaggccttc caaagacaaa gattgctgcc ggtatgatac tttccaagtg caaaggcgtc
    10021 tgataaccaa acagatggaa aaagcattgg ttcccctgac cgacgttgag ctaatatgtc
    10081 ctcaataatt cttgaagcat tcagtagaac ctgcggacac cgctcaaaga attttgtatc
    10141 cccagtatgt tcaaaataaa gactagccag taacgcactg gataacgaat tactcagaga
    10201 atgattgata cctcctgtaa attttgaacc aggaaactta acgccatatt gatcaaacca
    10261 tagaaccgtt ttttgacata actccggttc aaacatggtg aatggcaatg atgcgtaata
    10321 catatcctta ttccaaattc gattagtagc aggatagctg ccccaattgc tgccgacgac
    10381 ctgtccctgt cgattcatac cgaaactaga aaagctttga taaagtgcac gacgatagag
    10441 atggacaact ctatcgtttg gcattgacag ctgcccaaat agctgagcaa aatagttcaa
    10501 tgtttccttc agccattttt cgatatcaga ttcctgaaaa gtcattactt cttcaaaagc
    10561 atttgggtcc acaaaagcaa tggcgaagct tgccacttcc tgttgtgcga tcgtgtcctt
    10621 atttcctttt tgagaaatca acacattttg ctgatccgaa tacttctctt gatacgaagg
    10681 cacatctttc actgaaactt gaaggctttc gtgtgtttca ttcacaatgt aaacctgttg
    10741 aacaagcata cttaaacgct tattagccag tatgggagca aaggatacta caatgacctt
    10801 aaacgtcgaa taatgatgaa tatatctagg caaacagtct tgaatcacat cgacttcaat
    10861 acgatgatcc gattctgaca aatggtagac aatcccgttt acttcaacct ttaaggataa
    10921 cgaattggtt tgagagagct gtttattgac ccaaacacct ggtttttcct cagttagtaa
    10981 attttctcga taaaaggtca acgtttgaat cgtcccgcaa acatcaaggt ttgctaatgt
    11041 cttttgatta cttacatcga aggtcaatcc ctttacatca ggaatattcc gatagtagct
    11101 gtattcgttt gaagtagtca catttccgtc gtttaactca gcattaaaaa atgggtgttt
    11161 gggtgaataa attacttcat taatagtcat cttttccctc cttgctaaaa aaactgcagc
    11221 ctagtagctg cagcttccta ctatgcgatt ccaagtaatg acaataaaat tcccaagacg
    11281 accattccaa aaataatttt gatgacgccg acttttttat ctagtaattt gaaacagctc
    11341 aatgttacca ataacggtac aattccttta aaaatactat cgaggatttc ttggacattc
    11401 attagttctt cgcccttcat tgaaaagctc aaaacggtat tgaatttgac catactgatc
    11461 gtcattgcac ctaccatgat cagacccaag atactagcag cttttgtcag aatttcaatt
    11521 aacccgttcg catacagctt ttcaatatat ttagagccta atgaatagcc taaaacgccg
    11581 ccatagtatc gcatcaagta attaggtata ttgaacataa ctaaaaagat aattggtgcc
    11641 aaaatatttc cggcattggc taagttgatc gctagtcccg cggtaacgac acgccaaact
    11701 ccccagaaca atgaatcgcc aattccagcc aatggtccca tcaaactcgt tttgatcgcg
    11761 ttgatacttt ccacgtcaaa accagctttt tcagaatttt ccttctccat cgatgccgca
    11821 atccccatga caaaggacgc gcaaggaatg gtcgtcgaaa aatactgtgt atgacgttcc
    11881 agcgccgcag ctcgatcttc ttcacttttg tagaaatgat taatgaaggg catcatagaa
    11941 taggcgaaac caccgccgcc catcgtcacc ggattcaccg aagcatacat agtcatcgag
    12001 cgccaaaaca tcttccgaac caattttttc tcatctttag aaattccatc agctaatttc
    12061 attgaaagaa atcctcctct tcttggtcaa gcgttgcatt tacagcagca tcgctttgaa
    12121 ctgcatgaac agatatattt tttagttgta gatctctttg ggctataaca attgcaataa
    12181 tcgttccaac tgcggcaatt gcaatcaatg gtaactcgag atacgccata acaacggccc
    12241 caaagaaaaa ccacacagca atatttttac tccaaagcat ccgtaacagc atcgccatcc
    12301 caactgccgg cagcagattt ccagcgactg ttaatccgtt catgatgacc tctggaatat
    12361 tatccaagac gcttttgaca acatctgctc caaataaaac ggcaaagaat ggaataagag
    12421 cataaagcgc atagtggaca aaccacataa agaagtgcag attgatgatt cctttttgat
    12481 caccagattt tgcaaatttg aaaaatttag cagccatcgg tccacataca aaagaaaaaa
    12541 tcatattcat taactgtaat cctaatactg ctagtggaaa gacaatggga ataatcgcat
    12601 ccacgtttcc tcccattttg attgcgaacg ctgtagccag cccagcagcc aaagaaggct
    12661 ccgccgtact agagattccg atattgacga cacccataaa gatggcttct agtgacgccc
    12721 cgacaatcaa ccccgtctgc aaatccccca tcaacgctcc tgctaacgga gcaacaacaa
    12781 tcggacgact cagcatggac atcccgaaca tttcatgtcc agcaactcca ataaaaacgg
    12841 ccaaagcgac tacaaatgca tcatacataa tttttctcct tcctttttct aaaaatcact
    12901 caaaatagag atagctgaac gtttttccat cgccggtgta ggctgaaaaa cggtatctgg
    12961 ataaagttca actaattctt ttaagtcttg aatttctgac gtcgttaaca tcacttcctt
    13021 tgataaggtt ttcttatctt ccgatggcgg tgtcattttc ccagcattcc ccacgttcat
    13081 taattcgatt tccgttggga aagctttcgc aacttttacc gcatctttga cattagggac
    13141 taaaaccatc aagcgcatgt ccttcgcccg cggatctttt aacaaatgaa tcgcgtcatc
    13201 aactgtttta ataatgactt tgacattcga tggtgcagcc atcttcaatg tcatcttttg
    13261 aatatcgttg gtagccgcct catcgcttgc tactactaac ccggttaaat ttagactctt
    13321 tgtccaagtc atcgctactt gtccatgaat taatcgttca tcaattcgca ctgctttaat
    13381 catttttctt cctcctaaaa aaaatcttcc tcttcattta ctacatgtac ctcatcaaga
    13441 atggtcatct ttaattcctg ttggcattta tttaccagtt cctgaatatc ctcctttgtt
    13501 aattccttgg ttgccaatac tatttcgagc agaattggca gattaaagcc agacaaaata
    13561 gttacagcaa tctctttctc caaaaaggtt ttggtaatct tctgattgac acttccgcca
    13621 aataaatcgg taaaaacaaa aatctgatcc tgctcgttca cctggtttac aaatacattc
    13681 aactcctgtt caaaatcaca ctcatccaca taagcattaa tcaccgtaat ctcttgaacc
    13741 ttccccgtca gtaattgaat actgctaaga aagcctgaag ctaagtgacc gtgagtcgct
    13801 attagaattt tcttcatatg gttaattcct ttctttctgt tcacttttat ataagcaatt
    13861 cctgtgccaa cttttaaaaa cattggttta atcacgttaa ttcacattaa aaaaagtgtc
    13921 taacctaaaa aaaacagatt agacactttt ttagacactt tatttttgaa tatacggtcc
    13981 taataattca tagagcagcg aaagctcata agcattgact ttaaaattat atttcatctc
    14041 cattgattga aaaatacttc tagaaacata ataaaattca tcttcatctg gtttcaaatc
    14101 ataattcggt tcttcagatt tttctgtccg agccaagaat aaacgttcca tcatcaatgc
    14161 cgtatgcata tagagattta gttttacacg gccatttaaa gtgaaatgat agtaattttc
    14221 atatttagaa atgactgttt ccacttcact catcacgatc gttgggttca gaaagttcaa
    14281 acggtccgca attccttcca acgaaaagaa ttttactagc tcctgaatca agcggttaaa
    14341 ttctgccggc ttcatttcag gactcaaaat atgctccaaa ttggctgctc catcaccgtc
    14401 taagatgtca taaacattaa tattaggaat tgaaaaggaa cgtggaagat ccgaagtcgt
    14461 gatgaccaat tttgtcttgt caaaataagt ttcatcattt tggtcgatta attcacgtag
    14521 ttctctgtag tccttcgtga aaacttcgat ggattcatcc gataaatgat gacgaaaaac
    14581 atctttcaat ttgtccgaaa tccccaaacc cgacatacag gaaatgataa tgttgctact
    14641 ttgagaaaag ccttcaaagt attgaacatt gattgaatac ttgtctgctg catcctccgc
    14701 aatctgttta aatggcgcat tcatcgtcat cttcaagcca atatcaagtg caatggaagt
    14761 cgtcaaatta ttcagaagaa gtaactcccc ttttaaatcg ttcttgatct gtgtgtaaat
    14821 ttgatttaaa gagcccatat ctacgatcag cactagacta tctgtattca cttgccgttt
    14881 cacgaaattc cttgtcgatt cgacaatctc tgacacatta ctctctactg gcatatccaa
    14941 cgcttcaaaa acataattgc cgcataattg attcactact gcttgaatgc tgctagccgt
    15001 actatctccg tgagcaagca acaggccctt cagctcgatg gattcatcta cataatcaga
    15061 taaagtcacg acaataagca gtcttagaaa gttctcgtcc ttttctgaga gcggtggtaa
    15121 atgctccaag aactgattac ataagtaagt cgtccgaggt agatttgcag ctaatgtacg
    15181 atttatcccg gttaagtctg tcttatagct tggggaaatg acataggatc tgctaataat
    15241 ttctgcaaca ggtccactcg tcgtcaatcc gtacgttttt cctaaaacgg atacccagac
    15301 ctttctaaac atctgaatca tcaattttct actcattgat aaaaaagaat ccttatcaat
    15361 gtgatgcacc aaatgctgta cttcattctt tatcgaagaa aaattttcat tgaaattacc
    15421 ttcatttatc agctgagtaa ttttttccaa aatcgaatca taatcaaacg gtttgattcg
    15481 atccgttttt atcttttgat ttttctttat caataaacta gcaatcggca agctttctac
    15541 ttcggattcc agcatattta actgaatcaa gatctcattt ttcccaatcg ttaatggttc
    15601 ttcttgtgtt cgactataca catccgcaca actgatttta atgatattct tcagcttacc
    15661 aatattccca ctaaagggat gagataacat taaagacaag gcttctcttg aaacaataat
    15721 atctttttgt aaaacatccg cttcattttg aaaaaataat tgaatcagct ctaatcgttc
    15781 tcgaatgggg cgatcttcaa aagttggcaa cataacacta atttgaatac gccgatcaaa
    15841 ggtgtctaat aaataatttt cacctttttc cgtcgtggcg aaaataaaac gaaccttagc
    15901 agaatgccaa tttacatttt caccgactgg acgatagttt ccagtatcaa taaatagaaa
    15961 gagcttttct tgattttctt tcgataggcg atggacctca tcgagaaaaa gatagccgtt
    16021 gttggccgtt gccaataaac catcggtatc cttttcagcc cccgtaaagg cccccttctt
    16081 gtatccaaac agtgttgccg aaagcagttc gggattgtcc gcataatcgg cacagtttaa
    16141 gatagcaaat ggtgcatctt tttcaataat ttgctcatgg atagcgtatt caaaaatttt
    16201 tgtggctaaa aaactttttc caacacccgt ggctccattt attaatacac tgagtccgtt
    16261 aggcggatat ttgacagcgg ctatacattt ttgaatgacc tctctcagac ttccgtcatg
    16321 accaactaca gaatgaaagg cttcgtttat ttgctccgta ttatctacaa ccgcatattc
    16381 atctaaaggc ttccacaaaa caggctttcc cggtaatttc tcgacctttt tatcttccac
    16441 taatctcgtc aaataatggc tggcattctg acgagagata cctagattct tcgaaatttt
    16501 ctcggcagtg acagaagaca tattccctgt tagtcgtatc agctcatcaa aaactcgatc
    16561 tttagcgctc tttttcattt tttcctccag tttaatgtgt ctaaaagcat aataaactat
    16621 cggctgcaat atgtctattc tatatttgta ttccaatttt tactttgaaa cacaaatagt
    16681 cgaatatttg cctcagatat ctgacaaaat aatttatcta ccaatttagt ttttatctaa
    16741 tatgattttt atgttttttc atagctacaa atattgaact ggattcatat attagtttta
    16801 ttaatagatt acagaagggt taagtatatt tgcatagttt ccaattactt tatccttctg
    16861 taactttttc aatcttagat ttactagccc gtctcgggcc ctattttaca atggttttgt
    16921 ccacttttct ctggtagaaa aacgtggtag aaaaaataaa ataacacaaa aaaagcggcg
    16981 tttcatccgc ttttggtaga agattggaaa actcagatag aaaaaggacg atttttagct
    17041 ctttagaacg ttgataagtc cccattctta ctgagttttg ccattatctc ctatacatct
    17101 tgaattctaa gaataggtca ttatagatgc ctaggtattt cgatcctaga tctttgtaga
    17161 cttcaagatg ggagatcgtg tcatcagatg gatagaattg ttcgtccgac gaaatgtctt
    17221 ttggcaataa cgcttttgct tcttcatttg gtgtagaata accaatatac tctgcatttt
    17281 gtgccgcatt ttccggttct agcataaagt tgatgaaatc ataggcacct tctttatttt
    17341 tcacggtttt tggcataacg atattatcaa accaaagatt ggatccttct ggtgggatca
    17401 cataatgtaa atgttcattt tcccacatca tatcggcagc ttcgccagaa aatgtcactg
    17461 ccacggaggc ttcttcattg atcatataca ttttgatctc atctgccacg attgctttga
    17521 catttggtgt taaatgattc aatttatttg ctgcttctgt cagctgtgcc atattcttac
    17581 tattcaaaga atagcctaaa ctattcaaag aaagccccat aacttctcgt gcaccgtcaa
    17641 tcagcatcaa actgtctttt aattccggct tccacaaatc atcccaatgc ttaatttgat
    17701 ttgcatcaaa gaatttatcg ttatatacga tccctaaagt tccccaaaaa tatggaatcg
    17761 aatactgatt atctggatca aaatccaagt ctaaaaatcg tggatcaata ttatttaatc
    17821 ctttgatctt cgaatgatct aatggaagta ctagtttttc tttgatcatt ttttggatca
    17881 tatattcact tggtatagcg atgtcgtagt ttgtcccacc ttgctggatt tttgtgaaca
    17941 tcgcttcatt agaatcaaat gtttcataat tcactttgta tccatattct ttttcaaatt
    18001 tcgtgatcaa actaggatcg atataatctc cccagttata gatggtaagt acctttgctc
    18061 cactcatgcc acttgttttt tcaagttgac gaacaccaag cagcagcacc gcaataatga
    18121 tgagaatacc taaaaacaga gattgtaatt ttttcatcgc agccgtgcca ccccctcgcg
    18181 tactcttctg tttttcttca gccgttttgg acggttttct ttactaataa agtagtatcc
    18241 aatgaccaat atcatggaga acaagaaaac taacgcactt agcgcattga tttctaaact
    18301 gattccttgt cttgcacgag aatagatctc tactgagagc gtcgtaaagc catttcctgt
    18361 gacgaaaaat gtaaccgcaa aatcatcaag agaataggta aatgccataa aatatccagc
    18421 tatgattcct ggagaaagat aaggcaaaat gatatttttg actacttgga aattattagc
    18481 accaaggtct ctagccgcat cgaccattga atcgttcatt tcctgcaatt ttggcaagac
    18541 catcaagacg acgattggaa tgctgaaagc aatatgggaa agcaatacac tctgaaaacc
    18601 taatccgaat cctagtagtg taaagaaaat caagaaacta gcaccgataa taacatcagg
    18661 agagactaat aagatgttat tcatgctcaa caacgctgtc cgtgcacgtc gttttttcgt
    18721 gtaatagatt cccatagccc caaatgtccc gatgatcgtg gcgatcaatg cagataagaa
    18781 agccagcatg aacgtattca atacgataat gatcaaacgt gtatcttcaa aaacagcttc
    18841 ataattttcc caagtaaatc ctgtaaagct gttcatcgtt cctccttcat tgaaggaata
    18901 aaaaatcaga tagaagatcg gtgcatataa gaataaaaag acgatcccta aataaagata
    18961 cgaccattta aattttttca tttatgagcg acctcctttt ttcttctcgc ctgtcaggag
    19021 catcacgatg aacatagcaa tgattaaaat cacaccgatt gtagaaccca tcccccagtt
    19081 ttgtgtaacc aagaaatgct gttcgatagc cgttcctaaa gtgatcaccc gatttccgcc
    19141 aatcagacga gtcagcatga ataaagaaag agaaggaata aatactgctt ggacaccact
    19201 tttcacacca tttaatgata acggaaaaat cactcgtcgg aaggtctcaa agctattcgc
    19261 gcctaaatct ctactagcac tgatcaaaga aggattcatc tcttccaatg cattgaaaat
    19321 tggcataatc ataaatggaa tctcgatata cgttgccacg aacagaaaac tgaagtctgt
    19381 gaataatatc tgtttcggcc cgatcccgat aaaatcaaag aaatgattca cgctgccgtg
    19441 cacactgaaa atcccaataa atgcgtaagc tttaagcagc agattcaccc atgtaggcaa
    19501 aataatcagc atcagccata attgcttgtg cttcagtttt gttaaaaaat aagcagtcgg
    19561 gtaactgatg atcagcgtga aaaacgtgat caaaaatgca taccatacag aattgatcgt
    19621 catagaaagc gatgtccccg acgtaaaata agatagataa ttattcagcg tgaattgccc
    19681 attcatatca aaaaaagatt gataaatgat cattaataca ggcgcgatca cgaataaacc
    19741 gagccacaaa atataaggga cggaatagat cctgcgcatt gttttcattc ttgtcccccc
    19801 tattcttcgt agctgtcaag acgtgcatcg aattcttctt cagactcgtt gaaacgcatg
    19861 acatggatat cttctggctc aaaggccaat cctactttgc ttccttcttt agcttttttc
    19921 gttgaatgga ccatccattc attttgctgt tcatcataac agatgatttc ataatggaca
    19981 ccacgaaaca gctgagtatc gaccgttacg actagttttc ctttatctgg tgtcgtgatc
    20041 gtcaaatcct ctgggcgtaa aacaacttca actggttcat ttagacgcat cccgccatcg
    20101 acacattcaa attgcttacc aacaaactca actagattat cttcgatcat tacaccgtcc
    20161 acgatattgc tctccccaac aaaatcagca acgaaatgat tgattggctc atcataaata
    20221 tctacgggtg taccactttg gacgattttc cctttattca tgacaaaaat ctcatcactc
    20281 attgccaacg cttcttcttg gtcatgagta acaaaaataa acgtgattcc taatcgttgt
    20341 tgcagatccc gcagttcata ctgcatatct gtacgtagtt tcaaatcaag cgccgataac
    20401 ggttcatcta atagcaatac tttcggttca tttacaattg ctcgtgcaat cgctacacgc
    20461 tgtctttgac caccagacat ttcactaatc tcccgttttt cgtatccagg aagctggacc
    20521 atacgcaaag catcttttac cttcttttcg atatctgatt tggacatttt cttgatcttc
    20581 aatccaaaag caacgttatc aaaaacattc atatgaggaa aaagtgcata atcttggaag
    20641 actgtattta cttgtcgctt attagctggc aaatcattca accgtttccc ttcaaaatag
    20701 atgtcccctt ctgtagcttc ggtgaatcca gctataatac gtaggatcgt tgttttacca
    20761 cagccggaag gaccaagcag tgtataaaat tttccttgtt cgatctcaaa acttactttt
    20821 tttaatactg tttcatcatc atagcgtttc actacttgat caaatgaaat gattggtttc
    20881 cccacgtttt taccccctat attacaaata tgattgcgtt gcaacaatca atacttttgt
    20941 tcgttttttg agatgattcc ttaattgatg ttgttttgtc gcttgataat agatcgactg
    21001 tcctgctttt gccacataga ccgtctctcc taagcttaac tctaattccc cttctaatac
    21061 aaaaataaac gtttcagata atgaaggttc aaacgtctta tattctcctg caccttcaaa
    21121 agtcagcaag acaggctcca tttctttttc attcgaatca gccacgagcc attccaattc
    21181 atacccgttt tcttcatcga aataaaccgt atgatcttct tttgaataaa ccacttgcag
    21241 attcgctgct tcttgatgaa aaaattcttc tggagtcaca cctaagactt caagaataga
    21301 aaagaatgtt tccattgatg gtgaacttaa atctcgttcc aactgggaaa tatacccttt
    21361 tgacaaatct gtgcgttcgc ctagttcttc ttgtgtcaaa ttcttttgga tgcgcaaatt
    21421 tcttaacttc tcaccaattt ccataatttc ctccgaatcg aagttttcta atagtaaact
    21481 tcctgtttac taccactcgt attagtatac gaaatttatt gaaaaaggca accctttgta
    21541 aaattttttt tgctgaatat ttctttatct tttggaataa attctagttg tttgattctt
    21601 tatttttcac ctaatttttg tctaaattta cggaagaaca agaataaaag agtaggcttt
    21661 atggtatgat aaaagaaaat tttaaaagga gtgtctcacg aatgaacgct tttcgatctt
    21721 ttcaggtaat aaaggaacct gttttccata cagcaatcag tgatctgcca tttactgagt
    21781 tggctgaaaa tgaagtcttg gtaaaaattg cctattctga tgtcaattat aaagatgcat
    21841 tggcaatgag cgagtctggt caggtgatac gaacatatcc gatgactcca gggattgatt
    21901 tatccggaac tgtcgtccaa agtaacaatc cacgtttttc aaaagaagat cttgtacttg
    21961 ctactggttt tgggcttggc gtcacccatc caggcggtta tagccaatat caaaaagtac
    22021 caggagactg gttagttcct ctaccaaaaa atatgacgct gcgtcaagcg atgatccttg
    22081 gtacagcagg gtttactgct atgttgtgtg tgaacgcttt aatccgtcaa ggtatgacct
    22141 ccgataaaaa agttgtcgtt actggtgctt ctggcggtgt aggcagtaca gcgatagccc
    22201 tgttacataa actaggattc cagtcaatca ttgccttttc cagaaaagaa gagtctgtca
    22261 catggctaaa aagtctaggt gctttgcaag tcgtacgacc tgatgaattt ctgccagaga
    22321 caacaaaagc tttaggaaaa cagcaaatcg actatgtcat cgacacagta ggtggcgagc
    22381 agctaagcag tcttcttcca ttgatctctt ataacggtgc agtagcttta tgtggcaatg
    22441 ctgggggaat caaactaaac gcgactgtct tgccttttat tctgcgaaac atccagttga
    22501 ttggaattga ttctgtcaat gtaccaatag atcaacgtct ttctttatgg caacagatgg
    22561 cggatttgca gattgcagat gaactggtcg tccaagagat tactctggat caattgcctg
    22621 aaacagcatc taaactactt gctggaacac accaaggaag aacactagta aatgtaggtg
    22681 accataaatg aagattctaa tcacagctgg aggaacgact gaaaaaatcg atcaagtacg
    22741 tgctattaca aatcattcga caggacgatt agggcaagca ttagctgatt atttagctgc
    22801 caatccggat accacaatcg attacgtgac tacgaaacaa gcactgaaac cgaaaagaca
    22861 ctccaatata acgatttata cgatcgaatc tgcactggac ttatttttac aattagaaga
    22921 actaacgaaa aaagaacact acgatgcgat tatccacagt atggcagtca gtgattttac
    22981 tcctgctttt agtttttcag aagaacagct ggcaaaaaac ctacccactt cttcaacgca
    23041 agaagaactg gacaattggt ttgcggaaaa tgaacagaca gataccactg tttcaaagat
    23101 ctcttctaac accgagcatc ttgtgctcgt tttgaagaaa acaccaaaaa tcatttctta
    23161 tctacgtgaa tggcagccaa aagcaaaaat cattggtttc aaactattag tagatgtacc
    23221 aaaggaatct cttctggcag tcgcaaaaaa tagtctgata aataacaaaa ctgattttat
    23281 tttcgcaaat gatttgacgg aaatccatgg cgagacacac cacggctatc tcttatctaa
    23341 agatggaaca gtggaagaag ctcaaagtaa atctgagatt gctgcattga ttactgagaa
    23401 aattagactg gaggaatcaa aatgaaaaag atcttactag gcgttactgg aagtatttct
    23461 gcttacaaaa gcgctgatct tacaagtcaa ttcgtcaaat taggtgccca ggtagatgtg
    23521 attatgtcta aaagcagcac acattttatc acgccattaa ccttgcaatc tctgtcgaag
    23581 cgcgcagtcc atacagacgt catgcaagaa tcaaatccgc aggtaatcaa tcatatcgaa
    23641 ctagcaaaac aagctgactt gtttctcatc gcgcctgcta cagcaaacat tatcggcaag
    23701 ctagcaaacg gcattgcaga cgatcttctt tcaacagtcg caatggcgct tccagtggga
    23761 acaccaaaat tgattgcccc tgccatgaac acgaatatgt atcaaaaccc gatcactcag
    23821 cgtaatctgg aaacattgag atctgtcggc tatcaggaaa ttgaaccaag agaatctctt
    23881 ttagcttgtg gagattttgg aaaaggtgcc ttagctgata ttcagacgat catcgaacag
    23941 gtcatgctga ctcttaatga aagaaatgct tagaaaaaag gagtacttat gaaaaataca
    24001 aaaaacttta cattaactgc gatgttttta gctattttga ttttattagc tgttacgcct
    24061 ttaggtttta tccctatcgg tccaatcaat gcaacaacca tgcatattcc tgtaatcatt
    24121 gcttcgatcg ttttaggacc aagactaggt gcttttcttg gcggtacatt tggactgatc
    24181 agcatgatcc gtagcacagt tatccaaaca cctttgtctt ttgtcttttc tccgtttatc
    24241 ccagtcatcg gaacagaaca tggaagctgg aaagccttgc tgatcgcgtt tattcctcgc
    24301 atcttgattg gggtggttcc ttatttcgtt tataaaggtc tgcgcaaact aatgaaacaa
    24361 aaagcagatt ccgtttcttt attcttagct ggactttccg gttcattgac caatacgatt
    24421 ttagtgatga atatgatcta ctttttattc cagcaagatt atgccaaagt catcggaaca
    24481 aatttaaatg ctgtttattc agctattcta gcggttattt ttacaagtgg tatacctgaa
    24541 ggaatcgtcg caggtcttgc gactgcagca gtatgcaatg tcttgttaag atacttcaag
    24601 cacccttctc ttactaactg atagtcatcg tagccgatct ctgatttttt tctcttttgg
    24661 tgttactatt gaatggaaag atttgacgtc gaaaggatga tccaaatgaa aatttttgtt
    24721 gtaggtgcaa acggacaaat tggccgacac ctgataaaag atttagctcc aagttcccat
    24781 gaaatttttg caggggtgag agatgttgct tcccaaacat tagtaaaaaa agagaatgtt
    24841 tcgtatgtct cttttgattt aacttggtcc gtggaaaaaa tgaccgaagc attcaaaggc
    24901 atcgatgtat tgatttttgc tgccggctct caaggaaaga atcttctgca agtagacttg
    24961 gatggcgcaa tcaaaacagt gatcgcagct gaaaatgcgc atgtctctcg ctatctgatg
    25021 gtcagtgctg tttatgcaga tgaaccagca aaatggcctg agtccatgac agattattat
    25081 attactaaac attatgcaga tgaatggctg aaacgaacta acttagattt tgtcattctt
    25141 caaccagtca cattgactaa cgatgaagaa gttacttcca ttcaattaac taagcctaat
    25201 gagaaggcat caaaaacgat cactcgctct acagttgctg ctgtacttgc cgctctagtg
    25261 gaagaaacgg atatttctcg tacgactcta gtactttctg aaggtagcaa agagttgaat
    25321 acagcattcc aagaatgggc aaaggaggaa tagatccatg cgtgccattt caatcaagca
    25381 tcctggttct gtagatgtat tagaacaaat tgaagtgcct gttcctaaac caaaacaagg
    25441 cgaattactg atcaaagtcc atacagcagc tgtcaatcga acagatatta tgaaaagaga
    25501 atcagtctct ttgaaacctc cctaccctat tttgggagtc gaagtagctg gagaggtagt
    25561 tgagaataaa agtgatatct ccacttttac tcctgggaca aaagtatacg gattagtcaa
    25621 tctaggtggt tatgccgaat atgctgtgat gcctgccgat cgtgcaatcc ttctccctga
    25681 tacgctagac tatgtttctg ctgctggaat ctcagaagtt tttttgactg cttatcaaac
    25741 actttactgg cttggaaaat tacaagaagg cgaaactgtc ttgatccatg caggagcaag
    25801 tggtgtggga acagcagcga tccaattagc tagacagctt tctcacgcga aaatcatcgt
    25861 tactgcagga tcagaagaaa aactcgcctt ttgtaaagaa cttggtgcag atgaattgat
    25921 caactataaa aaacaagact ttcaagaaga ggtacgaaaa atcactgaag gtcggggagt
    25981 cgatgttatt ttggatttca tcggcgcttc ttactgggaa aagaatttag cttctatagc
    26041 cgttgatgga cgttgggtct tgattggtat gctaggtggg accatcgtgc ctgagataga
    26101 ccttggtgta ttgattggga aacgtgtcca actgcttggt acattattga cacctagaag
    26161 tgatcagtat aaagcagagc tcgcacaaac ttttatgcaa aaagctgggc cttttctaga
    26221 aaatggcaag attcggccaa tcattgaccg tacattccaa ttcagcgcag tcaaacaagc
    26281 gcatgaatat atggaagcaa acaaaaacat cggaaaaatc attttgaaga taactgaata
    26341 aaatcatgct gatcaaaaaa gaggctggga cagaagcgtt taactccaag aaataagaag
    26401 aaattcacga aaattgcttt tcaaattttt gtgaatttca gcttatttcc gaaggaattt
    26461 cttctgcttc cgccgtttat acgtgtttag agcgtgagac aaaagtgttc tttacttttg
    26521 tcccacgctc tttttccg
//
LOCUS       DACGLW010000033        26170 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.033, whole genome shotgun sequence.
ACCESSION   DACGLW010000033 DACGLW010000000
VERSION     DACGLW010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26170
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.033"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            complement(192..662)
                     /locus_tag="IXT53_001407"
     CDS             complement(192..662)
                     /locus_tag="IXT53_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HAQ4905241.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            992..2632
                     /locus_tag="IXT53_001408"
     CDS             992..2632
                     /locus_tag="IXT53_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HAQ4905242.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            2786..4108
                     /locus_tag="IXT53_001409"
     CDS             2786..4108
                     /locus_tag="IXT53_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HAQ4905243.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(4176..4610)
                     /locus_tag="IXT53_001410"
     CDS             complement(4176..4610)
                     /locus_tag="IXT53_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ4905244.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(4675..5511)
                     /locus_tag="IXT53_001411"
     CDS             complement(4675..5511)
                     /locus_tag="IXT53_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HAQ4905245.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLVNCYNAFVILQTLGESRLTQVKITETYC
                     SK"
     gene            complement(6253..6879)
                     /locus_tag="IXT53_001412"
     CDS             complement(6253..6879)
                     /locus_tag="IXT53_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905246.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            complement(7174..7509)
                     /locus_tag="IXT53_001413"
     CDS             complement(7174..7509)
                     /locus_tag="IXT53_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HAQ4905247.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            complement(7496..7780)
                     /locus_tag="IXT53_001414"
     CDS             complement(7496..7780)
                     /locus_tag="IXT53_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HAQ4905248.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            complement(7836..9359)
                     /locus_tag="IXT53_001415"
     CDS             complement(7836..9359)
                     /locus_tag="IXT53_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HAQ4905249.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            complement(9352..10527)
                     /locus_tag="IXT53_001416"
     CDS             complement(9352..10527)
                     /locus_tag="IXT53_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HAQ4905250.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            complement(10531..10716)
                     /locus_tag="IXT53_001417"
     CDS             complement(10531..10716)
                     /locus_tag="IXT53_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905251.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            complement(10682..12376)
                     /locus_tag="IXT53_001418"
     CDS             complement(10682..12376)
                     /locus_tag="IXT53_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HAQ4905252.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            complement(12373..12846)
                     /locus_tag="IXT53_001419"
     CDS             complement(12373..12846)
                     /locus_tag="IXT53_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HAQ4905253.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            complement(12915..13070)
                     /locus_tag="IXT53_001420"
     CDS             complement(12915..13070)
                     /locus_tag="IXT53_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HAQ4905254.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            complement(13204..13584)
                     /locus_tag="IXT53_001421"
     CDS             complement(13204..13584)
                     /locus_tag="IXT53_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HAQ4905255.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            complement(13588..13803)
                     /locus_tag="IXT53_001422"
     CDS             complement(13588..13803)
                     /locus_tag="IXT53_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905256.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            complement(13806..14213)
                     /locus_tag="IXT53_001423"
     CDS             complement(13806..14213)
                     /locus_tag="IXT53_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HAQ4905257.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            complement(14479..15954)
                     /locus_tag="IXT53_001424"
     CDS             complement(14479..15954)
                     /locus_tag="IXT53_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HAQ4905258.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            complement(15944..16792)
                     /locus_tag="IXT53_001425"
     CDS             complement(15944..16792)
                     /locus_tag="IXT53_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein"
                     /protein_id="HAQ4905259.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            complement(16829..17191)
                     /locus_tag="IXT53_001426"
     CDS             complement(16829..17191)
                     /locus_tag="IXT53_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905260.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            complement(17491..18115)
                     /locus_tag="IXT53_001427"
                     /pseudo
     CDS             complement(17491..18115)
                     /locus_tag="IXT53_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            complement(18112..18423)
                     /locus_tag="IXT53_001428"
     CDS             complement(18112..18423)
                     /locus_tag="IXT53_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905261.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            complement(18467..18760)
                     /locus_tag="IXT53_001429"
     CDS             complement(18467..18760)
                     /locus_tag="IXT53_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905262.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            18954..19601
                     /locus_tag="IXT53_001430"
     CDS             18954..19601
                     /locus_tag="IXT53_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ4905263.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            19662..20807
                     /locus_tag="IXT53_001431"
     CDS             19662..20807
                     /locus_tag="IXT53_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ4905264.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(21246..22064)
                     /locus_tag="IXT53_001432"
     CDS             complement(21246..22064)
                     /locus_tag="IXT53_001432"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905265.1"
                     /translation="MVQGLFGGGTSYEGQSISDIKEDVNNWKIYTENVKSQIVEKKEA
                     LIKNGFWNKIPWNFQQTINESIFCLGTFLSDFQIVESAFEENFISEREIKLLRKIGKN
                     SVQFNHSYGKYFKEEYEWREYGNPEFEIVESIYQHGRDFFVTLQDASNAAGRLEDYMS
                     NNSIYNKVNIFGNSYNTNIQQGHNNNMIVSIDDMSELLSKLDLLLENLNDSLPEEEVE
                     EAQEYIETIKDEAIKEKPKKTMLRFAVDSLKKLKWSTEFLASVATIAQLVAPLL"
     gene            complement(22164..23489)
                     /locus_tag="IXT53_001433"
     CDS             complement(22164..23489)
                     /locus_tag="IXT53_001433"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905266.1"
                     /translation="MENIEETFLKVLSPYISDSILRFQEQEKNYKFGFDIKNYNDSLH
                     VVFQYNKTGKIGIKEVHIFQIFLAKVNIEASKSKLNYTTLLDTNALFFSKKLLPYLHS
                     YEWALRKLIYLLAPAYFSDNWAEESIPNEIISSIKRKQIQAIGKFDSKNLLQWIDLYD
                     FEEYLFGENYILVTQENEENIVRYKEISQDQLLKLFQDNSHTISDSYSLWSEVFSKYM
                     DVKLEEIQTDMSLIREGRNIVSHNKEIEMALYQKLIKKLKRYTNQLEKAFQKILAGDI
                     AGEDLSDMSDDFEGYIGNYFSNIDLAGVTKLSESLSAMKVLNTDSMKKSMESIGQTLS
                     VQQEIMQNFDTEGIRRATEGINQSLQSIKILNTDSMKRSIESIGQTLSALSAQRAIMQ
                     NFDTKGIRSATEGINQSLQSMKIVNTDSMKRSMESIGKISSTNQVLKSE"
     gene            complement(23658..24587)
                     /locus_tag="IXT53_001434"
     CDS             complement(23658..24587)
                     /locus_tag="IXT53_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905267.1"
                     /translation="MRSDSRFFVSNLQDDELIRQIDSLLETITESDKRIFLNYVELTR
                     HIIELDKLFNVFRYNLTNLLKHFTIFTNDLIESTGEKLTEDQYYYQINALTINLISSA
                     KTLTESIEVCMKNFLAEKDFKSFKNKILSKPYDEHFSYRFLLHMRNYSQHGHLPVNIH
                     QQRVYFDLDEILAMPHFDFNGQLKKELDVLRKDIYNQFRDSPRISYVYTVAKFNLVIT
                     EIYFNYLKEIKPILIKLNDEKNELLLNTKFQLINSDGSSSDMLFYEFDGENYHCFNRK
                     DNSISMYADIKREVKKILKVEEQYYKEIEKRNQ"
     gene            complement(24835..25185)
                     /locus_tag="IXT53_001435"
     CDS             complement(24835..25185)
                     /locus_tag="IXT53_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905268.1"
                     /translation="MIFENESEEIGRMAEENKVVPFETLVINKEEFEQLFLDFFIQYD
                     RIYDFSSSLDLFELEAEIIENSREKNGKIIISVEKFNELFSETFEIDPDYYQPQEVFR
                     EFRNQIIRSSNIEN"
     gene            complement(25226..25720)
                     /locus_tag="IXT53_001436"
     CDS             complement(25226..25720)
                     /locus_tag="IXT53_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905269.1"
                     /translation="MFKVYFPFQWLVKGILLVLLGLILFGSYLTIKARHFPEEKPIKI
                     VTIDLKIGETYQADGVELTFKNIEVTEKRNEFAQHDRVLQVTYTLKNDTEKPIRPQQW
                     SIENKDGEELYEYAMILDNPEYIESDKEETVNYYYELPIERQDYQIVVRRANGYFVGL
                     PFTL"
     gene            complement(25713..26027)
                     /locus_tag="IXT53_001437"
     CDS             complement(25713..26027)
                     /locus_tag="IXT53_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905270.1"
                     /translation="MNYPYFKVSASEETKEIFNNFYNQNKGVFGSKANMFRVMVSNLP
                     VLASPSNNKFNDSESIKFEQKISELESMISNEVIEKLDDIDQKLSYSLKNKYKTEEKK
                     DV"
BASE COUNT         7729 a         5191 c         4136 g         9114 t
ORIGIN      
        1 tgacttcaca gtctcttcct taaacggtgt tcaaaaactt gcatctgcgg taataagccc
       61 aaccaaacga aaaataggag gagctatttt tgtttggctg ttcttattac ttgatgcagg
      121 gcaggttttt cacgacctct gattcacggt gtttcttttt catcaacatt tttacgaaat
      181 actgtggaag actattgaaa taagtgtgga ttgtttcttt gtgtttcaat cgtttcttgc
      241 cattcttcat ttaattttgg attaccgttt tcttccttga actcataagg tcctaattca
      301 aaaatcccag acagtatttg attcacgatt tcatatagtt cagtgtgatc agacacatga
      361 ttcttgaaga tcatctcatt atcaccacgt ttgaacctca cattcgggct ctcatgatga
      421 acctctatgc atttttctcc tttttttaac tgacggactt ctgaaaacat ctcataaata
      481 tcttctccag taaaaccttc agcaagcgcc agactgtcca caagaccgcc tactgtcagt
      541 tcagagatta ttgcttgatt ggaatctaat tttaatgtat agcttgtcgc ttctactacg
      601 ccacatgcat tgcaaccgcc ttctgatagt tcgctttttc taatcaattg aacttctttc
      661 attttctctt cctcctctta attttctact tatgtatttt tatcacctga cataggtgac
      721 tcctatcaac gctatcatgt gcttttttcc tcagaaatcc tatttatcca ttccatctat
      781 cccttattct tctacttttc tttctctagc atataagaga tacgcatagt attcaagcaa
      841 ggattgtcga tataaaaaga acaaatactg ctattttgat ttcttttggc tatattacat
      901 ataaataaaa tttatcatac ctgctctttt tgactatatc atcatctgac ttctatactt
      961 cgtttgtatt aaaacttagg aggaatcatt catgcttaca gggttaaact tattaaataa
     1021 tccttttcta aataaaggaa cagcttttac aaaagaagaa cgggcaaaat atggaatcac
     1081 tggtatgctt ccaagtactg tccaaacact ggaacaacaa tctgttcaag cttatggtca
     1141 atacttaagc aaacaaacag atttggaaaa acgtatcttc ttaatgaatc tattcaatac
     1201 gaatcggaca ttgttttata aattgatggg cgaacattta gttgaattca tgcctgtcgt
     1261 ttatgatcct gtcgttgcgg attcaatcga acaatacaac gaaattttct tgaaaccgca
     1321 agatgctgca tttctttcaa tcgacgaacc agaaaacatc aaagcaagtt tgaagaacgc
     1381 ggcagatgga cgagacattc gtttgattgt tgtgactgat gcagaaggaa tcttaggtat
     1441 gggcgactgg ggtgtgaacg gtgttgacat cgctatcggc aaattgatgg tctatactgc
     1501 tgctgctggt attaatccag cacaagtttt gcctgtatcg atcgacgctg gtactaataa
     1561 tgaagaatta ttgaataatc ccttatactt aggcaatcgt catgctcgtg tagaaggcga
     1621 aacttactat gaattcatcg atcaattcgt tcaatcagct accgaattat tcccagaatt
     1681 gttattgcat tgggaagatt ttggacgagg aaatgctgca gcgatcttag aaaaatacga
     1741 agatcaaatc actactttca acgacgatat ccaaggtaca ggaattgttg tcctagcagg
     1801 tgtattaggc ggattgaata tatccggtga acaattgaaa gatcaaataa ttttgacatt
     1861 tggtgctggt actgctggtg tgggtatcgc aaacatcctt ttagatgaga tgatccgtca
     1921 aggtgtacct gaagaagaag cacgccaaca tttctatcaa gtagataaac aaggtttatt
     1981 gtttgaagat acagaaggat tgactcctgg tcaaatccca ttcgctcgca aacgctcaga
     2041 tttcactaat gctgatgaat tgacaaactt agaagcagtt gtaaaagcga tccatccgac
     2101 aatcatgatt ggtacttcta cccaacctgg agcattcact gaagaaatcg tgaaagaaat
     2161 ggctgctcat acaccacgtc cgatcatcat gcctttgtct aatccaacaa aattggcaga
     2221 agcaaaagca aaagatttaa tagaatggac ggacggaaaa gcattagttg gaacaggtat
     2281 ccctgctgct gatgttgaat ataatggtgt aacttaccaa atcggtcaag caaataatgc
     2341 tttgatgtac ccaggtttag gacttggttt gattgcttca acagctacac gagtaaatgc
     2401 tgaaatcatt tctcaagcta gtcgagcatt aggcggaatc gttgatgtaa caaaaccagg
     2461 agcagctatc ttgcctccag tagcaaaaat cactgaattt tctcaaacga ttgctgaaac
     2521 tgtcgcaaaa tcagttgtcg cacaaaactt gaatcgtgaa gaaatcacgg atatcaaaga
     2581 agcagttgaa tctgctaaat gggttcctga atataaatct ttagaagatt aagatcgtta
     2641 tagtaaaaga acatacagaa actttgtatg acgtctaaaa agaaaaaagc gactgcggca
     2701 aaacagcggc cagccaactc ctgctctttt gtcgcttttc tttggctttt ttcatatgat
     2761 acagaataag aaaggatctg taaaaatggc acaaaaaaca acaactgcaa ctgcagttga
     2821 ggaaaagaaa aaagaaggtt tttggtccac aaaaatcacc ggcgtaagtt tgccacttta
     2881 tttgatcatg gtccttgtcc tgatcattgc aatggcttta gggaaattac cgacgaatat
     2941 gattggacca atcgcagttt tggttatttt cggtaattta tttcatttta tcgggacaaa
     3001 aatccctatc gtcaaaagtt atttgggtgg tggttccgtt tttgcgatct tcgcttctgc
     3061 tgcgattgct acatttggga tcttacctga atatgtagtg aaatccacag aaaactttgt
     3121 cagcaatatg ggcttttggg acttttatat tgctgccttg attactggta gcatccttgg
     3181 tatgaaccgt aatctattga tgaaagcttc tgttcgtttt attcccgttg cacttatttc
     3241 aatggtcgtc tccttttttg ccgtaggact cgtgggcatg cttatcggaa atggttttgc
     3301 aaattccgtt ttatacatct cattacctgc gatggctggt ggtgttggag ccggagccgt
     3361 tcctttatca acgatttatg caaatgtttt aggtacggat gcttcttcta tcatttcccg
     3421 cctgattcct gcgacagcaa tgacaaatgt cttagctatc atcggtgcag cattagttgc
     3481 tcgtgctgga caatcaatgc caaaattcaa cggcaatggt aaattaatgg aaaaaggcga
     3541 tcttggcgat ggaaaacctc gaactgtgaa accttccatt ccacagttag gtgttggttt
     3601 gatggtatca ttgactttct tcatcttagg acaaattggt aactattttg ttcctaaagt
     3661 ccatgcttac gcatttatga ttattatcgt tgtcatctgt aaaatcgcca acctattacc
     3721 agagtattat gaagatgcag ctatcatgtt caataacttg attgtcaaaa acttgacagc
     3781 agctgttctt gccggtatcg gtattgcgtt gctgaatctg aatgtattag cttctgcttt
     3841 gacatggcaa tttgttgtct tatgtctgac aagtgttatc gcgatttcag ttgtttcagg
     3901 atttgtcggt cgtttgtttg gtttatatcc aattgaatca actattactg ctggcttatg
     3961 caacaactcg atgggcggaa caggaaatgt agccgtctta tctgcagcta atcggatgga
     4021 acttattgct tttgctcaaa tgggcaatcg acttggtggt gccatcgttt tgatcatctc
     4081 tggtttcttg atgcaattac tttcttagtc aaaaaaacaa aaatacctga tcatcaagta
     4141 tcggcttggt gatcgggtat ttttttgata aagatttaca ggattttcaa aaatcttttt
     4201 tgcattttat cggaatgata accgatgtgt tcataaaatt ggtgtgcttc tactctgtat
     4261 tctgcagaat taagccttat tgtcgttatt cctcgttttt tcgcttcttc ttctacgcca
     4321 gtcatcaaag cctttccgat gcctcttttt tcagcatatt tagacacagc aagtgccata
     4381 atattgaaca ttggatcgct aaaaatcgat tcataggact ccgcttgtac atagccagct
     4441 gcttttttag aagtaggatc ttcataaacc aagaaaaaat ttttctctgg ctccttcagc
     4501 agtttcttca tttgtctcac cgtaacggtt aatggtatat cataccctaa ttgatccgca
     4561 ttgatttcac gtagtgcttc tgcatcttct aaacgcattt cccgtaacat ccaaatcacc
     4621 agccaatttc tgatttttta aaatgaattt ttttatcata gtatactctt tttttcattt
     4681 agaacaatat gtttcagtga ttttgacttg cgttagtcta gattctccta atgtttgtaa
     4741 gatgacaaag gcattgtaac aattgaccaa aagaaccacg aggacaatta cttgaaaaat
     4801 aaaggtcact ttttttggag acaaaaccct gctaaatctt gctccccaca aacctccaat
     4861 aacagcagcg gggataacat aaaacaacat agataaatca aagcgcataa agctacttgt
     4921 taaagcaatc gttaccaatt ttgccagttg agaaaagaaa atcgtagata gagaatatag
     4981 tgttgcttct tttattggca atgcaaacat cagcatcaac aaagaaacat tgactggtcc
     5041 accaccaata cctaaaaaac tagctaaaaa gcccagaacc cccccacata taaggtacca
     5101 agtccatgac gtcaaatgaa attttggctg atgatgcttg gtgtagaaga acgcaaaaac
     5161 caaggtcaat accgtcaaaa aaatttggat cagctgcaca tgcttctcgt tttcaaataa
     5221 ctgtaaaaaa acttcgaata caatgttgcc gactactcct ccaacaacag ctccactaga
     5281 tacccaaccg acaatctgcc aattcagtga tttcccgctt gatacttgtc gcacagtcga
     5341 aacaatggac atcgtgaata ccgcaactgt tgagtaaaaa gaaatccctg caacagaatg
     5401 ggcgccgata agatcaagaa tcggtttgat caacacgcct ccacccatcc cggaaatagc
     5461 gccaactgtg ttcgccagaa cgatcaccac aaaataaatc agaccaatca tcttgtattt
     5521 cctttctctt gtttatatct tttagcgatt gttactcttt tcttttatta tacagtaaaa
     5581 gaaacaaacg aggcttcgaa agcttttcta ctttttaagg aaatttagtg ctaaacgaaa
     5641 agaggttgtg aaaaagctgt cctgcatcaa gtaataagaa cagccaaaca aaaatagctc
     5701 ctcataattt tcgtttggtt gaacttatta ccgcagatgc aagcttttga acaccgttta
     5761 agaaagaggt cgtgagcctg ccgttcagct ccgagtcaca aggaacaatt tcaaaaaaca
     5821 gcttctcatg tttgttgaat attgtgactt gtgaccgagg agttggcagg agaacaccgt
     5881 ttaaggaaga gactgtgaag tcaacgtttt gacctgatta ttagagaaac aaatggcaaa
     5941 atagttcttc atatttcgcc gtttttcgag cgaatgccga ggggcagctg attgaacatc
     6001 gtttatttgg aaaaagggat tgtgacaaga cgtttgtcac aatccctttt tttatatctt
     6061 attaattcat tcttttgttc tattcttctt cttccgccat gaatgagaaa cccatatcaa
     6121 agaactataa aggttatcaa aacagcgttt atgactaaat gaattagagt tatctatcac
     6181 tatttttcaa gcgatttaaa ccaaatggca tacaatatgg catacaaaaa cttcaataag
     6241 ccgtgggggt ttttaaattt cttcttgttc tttaaaatca atgagtggag ctacataatt
     6301 tgaatccact ttttcaaacg tcactttttg catcgacgaa tgttttcttt cccaagataa
     6361 cgtaaataga tttgactctg gacttataaa gtctacttca ccttctagta ttactttctt
     6421 atgcaaatat tcctctaata aatatagact tgctaaagca tacaacacat tttccaaatt
     6481 tgcttcctta taatttgaag aacgaccatg ttttactctg ttatatattt tccaccaatc
     6541 tggtgtatct tcaggccacc catctccaaa aggttttagt gaagtcaatg ttaaagaatt
     6601 cataacccta acaccattat ccaaacacat taattcgcta gatttaataa cagctttaaa
     6661 atcagttatg cttttattgc tactgctatc aaatccgcaa agttctttga aaataatctc
     6721 acactccgaa ccaatcgcta gtagtagttt ttgatattca atagagaatg ttttgaaatt
     6781 attctcatcg agcatcacaa aatctgtggt attaataaac tcattttcta aatgtagata
     6841 atacttccaa taaaaatttg taaactctgc tttgtccatt aaatcacctc ttttgaatta
     6901 taacaaaaaa aacacgtcca caacagacgt gttctttgat ttctacacaa tcagcttgcc
     6961 aaaaccatcc aatcagtcct agcatgcctg tattaaaatt gaacgttcat catctatcat
     7021 ctttcttagt ccagcatgtt tgcaaccaca cctacaattt tcagtccaac aatagatttc
     7081 gactagctaa gtcttgcaaa attcaggcag ctaacccttc atttactacc ttaattatac
     7141 taaataccca caacgtttac aagtgtaatc attctaagaa atctgtttag ctatgatcgt
     7201 tgtataatct ttaaacgccg ttccttcatt gattgaaata attttataac tcttgttttt
     7261 ccaagctact ttcatatcgt ttgttagttc ataatcttga tcgtatcgaa taataaacgt
     7321 caacgttcct tcaagaatgg tacctacact agcaagcacg tcttttagca tttgtgaacg
     7381 cacattagcc caacattcaa atacagtttc ttcactatcc accatctgcc catcttcatc
     7441 ttttacccgt tttgtctttt taaacttgac tctctcagtc aattcattta cattcttaat
     7501 gattggcata ataagcacct ctcatttgtt gtactaatgc ttgtaccgtt actggtatcc
     7561 gttcgctgct ggcttcttga cggttcaaat accaatgttg agtaagtaat gacaccgcca
     7621 aatcaaattg ctgataatta tccatatctt tatccgttgc gctactatca atagcacttt
     7681 ttatatattc gcctgctgta tcaatcaatt gttgaataaa gtcatcatca atagtgtgat
     7741 cgatccgcat actttttttt atgctatcca aaattaccat tttatctctc ctaatgaaaa
     7801 aaaggcagtg ggataaaccg ccgcccaata tttttctata cttttggtgt tacaactggc
     7861 gtaaactcaa tatacactga agcattttga tcaattttct tataatcgtt gcgaacgatc
     7921 actgcaagtc cttgtgagta gtaatcgaat ttttcccatt gagtcgttac ttgatttcta
     7981 cgagcaacaa agatagattg tgcaatatcc cccataatca tagggaatgt tccagcttta
     8041 ggattggcaa acaatgtatc tgcaatcaat actactggca taccgaataa tgatttacca
     8101 ctaggtgctg tcacatcggg ttgtaaaatg taacgtccat ctgaatcttt caatgtatct
     8161 aggtggtaat atccgctttg gtttagaatt accattttat ttaatgctgg gtctaatgcc
     8221 acattgtata gttgtttcaa atcgtccaac gtagcagccg ttttcttagt gaatgttttt
     8281 aataaatcca tgatatgctt attgtcagtg ttttctacca atttcgctaa ctgatctttg
     8341 acctcttgta caatattcac tgctgaatct tccacaacct catttgataa ggcaatctta
     8401 ccagcacgag tttctacttt ataatcaact gaagtaaaca tttcagcgtc aatatcacca
     8461 atttcagcaa gttcagcttt tgttgctaac actgcttgtt ggttagttgc tactggatat
     8521 ttaccttgtc catttgatac tgtttttact gttgcatatt gagctaagtt ataatttgaa
     8581 cgtttcaaat caaagacttc accgattact tcttcgggaa caactgccgc tgcatttaca
     8641 gtagtgactc catcacgcac ttcgccttgt gaacggatat attcttcata cccacgcaat
     8701 tctgtttttt cgttatcgat taaagttttt tccatgttat tactctcctt ttgtgcttta
     8761 gaagtggatt ctaagctatt gtttgtttgt tttgcttggt tgttactcat aaacgattcg
     8821 taagaacgct tatttacttg gacatttgat gaatcataag cgggaacggt cacgacacta
     8881 atttcattaa gtgctttgat tttatctatt gatcgttcaa ttgtgccatc ttcttttttg
     8941 tcgaatgaat ctaatccaag cacgaaccca aaggacatag aatcaacaac cccttttgag
     9001 atattctcat acacatcatt tgaataggtc gtgtcggtta gctctgcttc aaaatgtaat
     9061 cctgtatcat cgacattcaa tttcaacgta cctgctttga cactagctag tggctttgaa
     9121 taatcgtgtc catataataa gaacacattt gataaatcca cctcttttaa tgcttcgggt
     9181 gtaatcactt ctacaaagcc gcctaaatct tttgacggtt gcccgaattt caacgcatag
     9241 cctgaaatag ttttgccgtc tgttggttgt ttttcatttt ctttctctac tttgggagaa
     9301 tcggctgtta gctcagcttc ttctgtcagc cgtttttcct tttcttcttc attattcaac
     9361 tggtgtaact ccttcctgtt cttttaattg atatttctcc aacgtatcta aatacgtaaa
     9421 gttcaaactt gctagtaaac gatcgccacc atccatgggg ggtagcccca ttttcgaccg
     9481 cccctcatta atcgtgagaa gtgacccttt gacctgatcc aatacatttt ttacttttgt
     9541 ttcaggatca gtctctagta actgatcgga attaaagcgg atattggttt caagtttcgt
     9601 atctaattca ctcacaaaca cattaaagta atgaatcagc gtactttgga tatactggag
     9661 attactttga acggtgctag aatgttcatt ttcaacgcct aaacgctcaa tcggtacacc
     9721 aaacgccttg gcaatttgtt ttgtgttcca atcattggaa ttgataagct tcaaaacatc
     9781 ggtgtttatt tctaatgttt tgtagtccat tgtttcatca agaatgatcg ttctaagagc
     9841 attatctcca ctacttgaac cgttggcttc ttcaaacttt tcacgaattg cgttctttgc
     9901 tgatccgtct aaatctgact tatgaacctt caaaatacct gacccactga caccacgagt
     9961 aaagaaattg tgcaatgtgc ggttgccagc ttgttgaatt ttcagttcat cacgtaacgc
    10021 ataaagtggt ggtaatcctg ttaaaccatc ttgggtgaaa tatttgaagt gcaaaatatc
    10081 gctagacttc acacgtcttt ttttatcacc aatctgataa gacaacgtac cattatctaa
    10141 ttgagtaacc gttacacttg agttaggtaa taagtgaatt tcttctacct tttcaccgtt
    10201 gcgtttaatc tctgcaaagc tattaccgtt tagcaacata ttgaccgcca aagcaaactt
    10261 taaatgccaa ccatccattt ctgaattagg tttctcgttt agtaacttca ccagttcatt
    10321 atcagcttgt ggcatattgt ttttgaccaa ttgaatcgga cttgaagcaa tatcactagc
    10381 aataattcga acggctgcaa acacatcact atttcttaat gcacgaacgc ttgtaaagct
    10441 ggtggtgtaa tcatccgatt ggattgatac cacatgttgt aggaaaggat ctcccgttgt
    10501 attttgtctc gctttaaaaa atgccatctt ttaacctccc ttctgttctg tatttagcat
    10561 gaccgctacc ccaataagca ctatgcccag cgctaacaag ccatagtaga catttgttag
    10621 taaggtgata gcaaccaaca cacacaccaa tccaagaagt aaaagaacgg tatgaatgta
    10681 attagaaaga aaattcttca cttgtataaa attcattgtc tgctttcgct ccttccgttg
    10741 tggtgaagta atccattgca aacacatagg cattgattaa tgcagctatt ggatcaattt
    10801 tgttactgtt ctttgattta ttaatctgaa tgccgttgtt atccgttttt aaaatagcgt
    10861 tgttcactgc atgggtgaga atcgtatttt tattgtgaat aacgttgcct tcataaactt
    10921 gttctctaaa agttcgggtc ggaacgttta gagtaatcgt tccttgtctt acttctaaca
    10981 ttgggtagtt ggctttttca gctttactga taagcgaatt agcgttatac gggtcataac
    11041 aaatccccat acattccaaa tcattttctt gaatcaaatc ttgaataaac tgaaatactt
    11101 ggtcatagtc cacgattcca ctttctaatt gagtaatgga acactcacca gctttttcta
    11161 gttctcgata aggcaaaccg tcgcgctttt ctttgtcttg aagcccatat ttggtggcta
    11221 caaagctatg tgaatcacaa taaagttcgc cgttatctag cggtacgatc catgaaacgc
    11281 tagttaaatc atctgtttta gataaatcca ctccgatata aacgggcttg cctgtaatat
    11341 cttgtggttc tacttcggtt gcctgccagt cttcagcaat catatagctg tcctcactcg
    11401 cttgtctcca taaattgaag tttttcacca atacagaatt aaggttattt tgtttcaaag
    11461 caaggttcac atcatcttga atggttggaa tcattacttt tttgatttct tcactttcaa
    11521 aaatcggatt ggctttgatc catttctctt gatcgtgaat ttcttcggga tcgtccaatt
    11581 cccatatcgc tataaagtac ctgtcggctt gttcttcgcc ttttagcaca cgatctaaca
    11641 ataagtattc ctcgtacata ggaacgttta aatcaagccc agcggtacta ataacacaca
    11701 ataagccgtt cttttgttgg gtcattcctg acttaatgac gttgtacgtt ttacgagttt
    11761 ttgcttcgtg ccactcgtca agaattgcgg ttgttggtgc aaacccatct agcgtgctgg
    11821 tttcactagc cagtgccata gcgaacgaat tagagggcaa gtgctgaatc ttagagttca
    11881 taattttcag ttgtggtctt aaaaacttac tagacttgac cacgtttcta agtgaattag
    11941 acagcatatc atagcccaat ttagcttgtt tcaaagcgtt acttacaaac aatacttgcc
    12001 ttgcttctgc tggttcttgt tccataatca acgcattggc agccatgcca cttgcgatat
    12061 acgttttccc attcttacgg ctcatactgg taaacgctcg attgaatcgc ctgtaattcc
    12121 cttctttagt acgccagcca taaagactgc ctagaatgaa tttttgaaat cctaacatct
    12181 tgattgcttt accgtccgta gatggtagca actcaatgaa tttaatcgct ttggtggctt
    12241 gttcttcatc gaacacataa gggaagctat ctgacttaga acgctccaaa tcgcttatat
    12301 gacgctcaca cgcttggata attttcttgg gtgcaatcag cttacctgat aggactttct
    12361 caatgtaatc attcatgaat tcatcaactc tttgaacggg tcttcgggtt catcattttt
    12421 aaccttgttc aaagctagtt ttgatcgtga ttctaaggtc atacctagtg aggtggcttg
    12481 ttttttcaaa tcactcatag cttgtgattg aacccgaacc gctggattgg ctttctttac
    12541 tccgttttcg catgtgataa acgtacctga ttttcttatc tcggcttcgg ctgttttcat
    12601 tcgagcaaag gctaaacaat aggctaccaa tgaaccataa tctgcttcac tcaatggata
    12661 atcttttttt aatgttggta cgatacgatt ccattcactt ctagcgctcg ctgccatcca
    12721 gtcagggggc gtgatattta ttaatggttg ctgcttgaac aattcttctc tagcgtccac
    12781 acggattgct ttttcttcac ttgatatatt gccttttgta tcgtgtaata ttttagggtg
    12841 tcccatgttg tcctcctttc agacaaaaga aaaaggaacg taagcaatta agctcacatt
    12901 ccttcgtttt tcgatcagaa tttttcttta tctaatttat ctatataaat gatttttccg
    12961 ttctgcattt gaatggtcac ttgaccaaaa tgaggtaact caatggtgcg ctgtttccct
    13021 gttttatcag taataacaat tgtatttttc aataaatctc ctactttcat tatatacttt
    13081 ttcacttgtg attacaagtg taatcgttat ttgtaattac ccttatgcct tttcacaacg
    13141 gttatttgta ccaaaatgag taggcatcga ttgtcagtaa gtttcacact atgccccctt
    13201 aactcaaagg gtactctttt ctcgtctttg tctttcattg gctgtcttgg aattatggtg
    13261 tgcatggcat aaagaacgaa aattagtttc atctaaccgt ctattccaat catcttttag
    13321 ttcaattttg tgatctacaa catcagcttt ccttatcaag ccttcttcta ggcaatcctc
    13381 acatactgga tgatttaacc gatacaagta tgaagccttt cgccatgttc tactttgata
    13441 gaattgaaaa tatttacctt cgctttgctt tctttcttca cgcttaattg ccgtttgctt
    13501 tgctttatgc ttcccacaat acttctgcct ataatcaatc aatgttttac atccagcatg
    13561 attacattgt tttttaactg ccatagatta ccaaccaaag cgatctcgtt cgtcagaatc
    13621 atcttgttcc cattttgatt tgtattcaat ataaggcgaa actatcttca agcacgttcc
    13681 gtctttaaaa ttaactttag tgattttatc ttcctcaaaa ctaataaagt ctacatcttc
    13741 ttgagtgttt gaatactcaa tgtgtttctc accatcgaag tatctaatac tttccatatg
    13801 cataattatt ctacctccaa taaattagtt atcaattcat catctgttag tagcaaagcc
    13861 aagaacttat catggatgtt taaaaacaaa ttatcctcat tcgatgtaag ctctcgttgt
    13921 ctcgctcgat catataggtt aagcagacaa ttaaattcat ccattagctc acctgtatca
    13981 tcatagttgc cactatctac caatgatccc actactagcg attgacacca cgctactgac
    14041 tcctgcagcc aatcaggttc ttcttccatt atctttaata cttctggatc aaatgtggaa
    14101 taatcttctg ttaccaatcc cacactaaat gccagcgtgt tcattagaaa ccgatccatt
    14161 tcatctgtat attgaaagtt cggtgctttg tttgttgaaa taatattgag catttacttt
    14221 cctccttgat tcgtaacacg tggtaacacc tacgtaacag gtacccgtta ccactcaatc
    14281 ccaacgggga caagggttct agcgttttgg taacaggtaa cgggtaaaat ccagtaacat
    14341 tattccccag catattagct aactatttca aaagttttaa ggaaataagg tgttaccccg
    14401 ttaccaaatt gctgtatccc ttggggcagt aagggtaaac tagtaacggg ctaggtgtta
    14461 ctgacccgtt actacccgtt actttttgta ctttccagct cgatttttat tttcataggc
    14521 atttgactta ttgtaaaatc ctcgtcgttt atttcttttt ggcagttgct tccgttccca
    14581 accaggaata ctgctaataa tcaaggcaat ctttttagtt tctgcatctg atcgaccatt
    14641 caataaatcc tttgcttgtt tatcaaaaac agcttccaaa atatcagcgg tgagtacgct
    14701 atcaactaaa taaaactctt gttgcatagt agctccatta tagaaatgct gtaaattttt
    14761 ctcattacat ttactgtcag gatagttctg ataataagaa cgtctcgccc acgctggagc
    14821 ttcttcccat ttatacggga tttccatttc caaatattta gtgattgctt ctttaattgg
    14881 attctccatc ttggcatcct cttgatagtc ttttgcaatt tctaagactt ctttatcttc
    14941 atcgggacta aaatagattc gttgtccttt atcaaaaagc acctttgctt cagctaacac
    15001 ttgaagaaaa tattcatcct ttgttttaaa cggattcatc tttggcttat tcttacaagg
    15061 caaaggataa aaacggcggt ttcctgtatc atctttcaaa tactgcccat cattcgacgt
    15121 tccaataaat acacattgcc ttgcccaagc tatgacattc cgaccataag gaggtctaat
    15181 tttatcgatc ttggcactga tgaagttttt tatctgttca attttagttt tgttcattga
    15241 agataattct ccaagttcaa taattacatt gccttgaagc tgcatataat catctttctt
    15301 ttcacctagc gaatctaatg aatcggtgaa aaaatcatcc gtgtaaagta atgctggaac
    15361 ggtactctta ccaatccctt gcggtccatc caaaactgga acgatctcaa actttattcc
    15421 tggtttatag atacgtgcga ctgatccgac tatccaacgt tttgttactt ctcgtgtgta
    15481 aaggttgtcg tctaccccta aaaggtcaat aaacagtgtt tccagtctag gctgttgatc
    15541 ccatttaact ttttcgatac gttcttttac tgggttgaaa acattctttc tagccacatt
    15601 tactactgct ttgtgtattt tgttgtcatc aaaaactaca ttgtagtttt cttcaatata
    15661 tgccgttaag gcatcctcaa aactgtcttc ccattctcca gcggtacgat tgatccaagg
    15721 agaatctttt aataggtgaa tgtatccgct aaattcgtta taagcaattg ttccagctaa
    15781 attgctatca ttggttaaaa tcaattctgc attcacaaca ctgttacttt taatctgatc
    15841 gtctttattg catcttaatc gtgacatcca ggttttcaat tcaatgatat tgtcttcacg
    15901 ctcgctggct tcgggaaagt ctaaaatact atctaattta tgcttatgag ccatgagctt
    15961 tccccctcaa atctttttta atcatcgatc gaataatttg attcacttct ttatctttta
    16021 gcggtggttc attgtacgca tttgctgtat aaatcaattc tgctaaagtt tgattatcta
    16081 caccgtgcca tagtaaccaa ccgcataact tagttaaatg atcgtttcta ctaccttcgc
    16141 cagcgccttg aacaatcata tctaattttt ttgctgtccc tgtttttgct acgggtcggt
    16201 attgagtctt tatttcttgc ggttttttta gcagtaggtt cactagccaa tcgggagctg
    16261 gtgcaatatt atttagagat acgttttcaa taggttcata tgctctccca acacttcttg
    16321 aaataatcat agttggcgct attggaactc ctttagtgat aatatcaatc ccgcttaaac
    16381 ttttaccttt atcatcacgc ccgaagatat cagcaaatgc acctgtttct tgttggataa
    16441 ttacaccatc aggaatttta aagaagaaat ggattccttt acctggtgtg atttccgtat
    16501 aagtagaagg aaatggatca tacattgaag caagtttttt ataattctca acgccgtttc
    16561 gtgtttcaga atgttgatct aggtcaacta ccaccatttg atgatcttca agcattaacc
    16621 caatatttct agttgcatct tccgtccacc attcattaat ttgattagaa tcgttagttg
    16681 catcattcca accactagag ccttttattg gaattttagt tccaggagat aagggataaa
    16741 ttgaccatcc cgacttttgt aattccgttg ctgcttgata attatttgcc aataaacctc
    16801 tcctcctttt tcaaaatact ggtataaatc attcagacac ctccatgagc ttcaactgct
    16861 caagttcgta tttcaaccca tcccgaacaa attcagcacc ttttaattga ttctgatact
    16921 ttacccatgt attaaatgtt ttcacccttg cttcttgcat atcagtaatg tttaaccatg
    16981 cggttaaaat ctgatttttt agctcgttga tttctcgaca aattattttg tgttctgcat
    17041 gttttttgag tacatacaaa actgttagtt tttcattgaa tgaatttcca aagtaggttt
    17101 tcccgatatt attttctagt tcagcgatcc cttgattaat ttcctctaat tcaaggctat
    17161 cgagttggtt taatagtttt gttttgtaca tctacttatg ccctccagtt cttaattgat
    17221 agtcgtactc gtcataagca gtgaagagat ataacgctaa tcctgcaaag atagctattt
    17281 ttgcaattaa tggtaataag ccgaaaattc cgccgattaa aatgcttcct ataattaagt
    17341 ttcgtttcat aatggttccc tccgattttt taatatggta taatcggaac aaagaatatc
    17401 gggttttatc ccaattaagt tgcctttatc ctgtccgcca agactgttat ggataaggct
    17461 ttttctttgt tctcgatcat gttaatgctc ctaagttgtc tgcaattcag cagaacgatt
    17521 ataaattttc tcactcactg aaaactgcaa cccatatttt tctttaacag tcattaattc
    17581 tactgtctca tctaaaatag gttcacgatc aaccaacatt tcaggagtca tatctttctt
    17641 ttttaccatt ttggaatgac cataaatagt tgatacagct ttattggcta tcacattcgc
    17701 tttaatgtag tctactggtt ttggttcagt aatagcgtgg cttaatttag tcattgcttc
    17761 tttttggtgt tctttgtcta gcatgcggaa tacttgaaag ccttcaagtc ctgtggactg
    17821 gcgtagttct ttgatgatgt cgaaaatcca gtcttgaaat gcctcggctt cttttttacg
    17881 gcttttgaat gccatgcggt aaatattttt tttcgttgat gatgtttaca tcttgtgttc
    17941 tgcccaatga atcgatgacc ttactagtag taaccccatc tttaggcaaa gagtgaatgg
    18001 ctctagtaac ctgtttaaga cctaacgctt ccgcaacatc tttcgccact gcccaccatt
    18061 catcattaat atcgacaaat cttataatgt gtccgttcca aatttcagtt ttcattcaac
    18121 gtcctccagt tcaagcccta gcaagctcgc taacaactca acttgctgat acaaaatttc
    18181 ttgatactct tcaatagaaa gtcgttcttt aaactctgct tgtactaaat cgttcaagcc
    18241 acgcaatact ccgcttaccg ttggttcttg tggtagcaat ttttgatagt ccatttgttc
    18301 gtacaaacat tcaaacgttt cttctagcgc tttgccttcc aagtttgcta attctgccgc
    18361 tgtgtcttct gtaagaattg ttgataatac tttttcgatt gctttatttt gtgtcgattc
    18421 cattattatt tcctcctatt aatccctttg cccccacggc ttgcgtttat ttttggtatt
    18481 cttccataaa gcgatcacat tcatcttgtg aaattcttac gactccatca actataatca
    18541 actttaaacc gttaggaaca tatttctgca tcatagtatt gtatgaaacg ttcatatact
    18601 tagcagcttg tgccttgttc atatattttg gaaactggta ctttttttct aataatgaaa
    18661 gtaacgttcc ttgagtttgc tctacttgtt gaacaactcg atcgtcaata attttaaaag
    18721 ctggatcatc aagcgtgagt ttgtcaattt ttaattccat atttatcccc cctcgttagc
    18781 atacatttct atacaaagat tttaaaaaaa tgcaagaggc tcttgaatac atttctatac
    18841 ctcttataaa ctcaatatac catcatacct tttcatttgc aagaatttta acatactttt
    18901 ttgaactttt atgtttcaca catatttaaa tcgtgatata gtagagaaaa tatatgaatg
    18961 gtggtgaaaa tgtggctaga aaaaaatacc ccgaagcagt ggagacagga caaagaatta
    19021 gactggctag aattgaaaag ggactctcaa tggaacaact tggaggtaaa ttatccccac
    19081 cggccagtaa aggtgccgtt agtaattggg aaaatggcta taatcttccc aataacacca
    19141 ggcttaaaca actttcaaag gtattagatg tatccactac ctatctatta aacggatctt
    19201 atacattaag agatatgcat atgatgcccg aaattaagcg aaatgtgctt gaaaagcaat
    19261 tgaaaaaaac tattgaaaaa aataactcta taattgattc taatctttct aatgctatca
    19321 ataacatcga agaagataac ctgactgtct cacagaagat ttatttaaca aacgcaataa
    19381 aatatcttaa aacaggatct cgaattgaat ttcctgacgg gagtacccca attgcttatc
    19441 tcgcagctct tctaaatacg cttttacgat atgactcatt aattaatgat tcagaaaaaa
    19501 atacagatga gaaaaaagag tatcaagaat tttatgaaaa tgatcttcta aaagagttca
    19561 cagctcttgt tactgagcta aataaacact attcaaaata ataataaact gcctcctcca
    19621 accgccccca cggcttgcta cactatggag ggaaaactat tatggcaaca ttcaagcaat
    19681 atgaaactaa aaaaggtaaa ttttggctct atgaagccta tcttggaatg aacaaaatga
    19741 ctggcaaacc tgataaggtt cgtagacgag gttacaaaac aaaaaaagaa gcccagcttg
    19801 ccttgtcacg cttgcaagtg gactacgata aaaacggtat taaaaaagcg aacaacgaaa
    19861 cgtttaaaga agtttatgac ttgtggctag aaacatataa aaccactgtc cgagaagcca
    19921 ccttcatgaa aactgaaatc aagtttagaa agtggattct tccaaaatat ggaaatcttc
    19981 gtgtgaatga agtcacggta aaaaaagccc aacaaattgt aaatagctgg gctgaaacta
    20041 ctgaccaata caaggtactt cattcaactt ctaagcgtat ttttagctac gctatcaatc
    20101 ttgggattat tgagactaat ccactagaca acgtattaat gcctaaaaga accaccacta
    20161 agcataaaga aaaggtcaaa gtttacagta aagaacaact gatcaccttg tttgattacc
    20221 taaacagcaa acctgaaact tatcgaaacg actttgataa aacgttacta cgctttctct
    20281 tttatagcgg ggttaggatc agtgaagccc ttgcattgaa ttggtcagac attgattttc
    20341 aagaaaatac ggtcaccatc aataaaacac taagccaatc caaaaatggc tataaaatca
    20401 gtgatcctaa aactgattgt agtgctgggg ttcttccact agatgaaaga acaatttcta
    20461 tattaaaaaa atggcagatt aatcagcgga aatatatgct gacacttggt gtaactgatc
    20521 cgactatgat tttttgtggt atctataaag aactaatcac acaccacgct atttatgcaa
    20581 gattgattac tatttgtgaa aaagctatgg ttccatttct cggtgtgcat gtagcaagac
    20641 acactcatgc ttcaatgctc ttggacgctg gagctactat ggtagaagtt caaactagat
    20701 tacggcatac aaaaattagt cagacgatcg attgttacgg tcacttggca aaagaaacga
    20761 aagaaaagac tgttgaaaaa ttagtacaac acttaaatat tgactagatg gcatacaata
    20821 tggcatacag taaaaaataa aaaaaggaga aaccctatca tatcaaggtt tctcctagct
    20881 ttatagtcta ttcttcttct tccgccatga agttaagctc attaaagata attgacgata
    20941 gttggtattt caacgatttt tgaatagaac taatcacttt attaagtcta attcacctta
    21001 agtggttttt tagtcgtatt caaaaacgta ttcaagttgc gtggcgtgct tttgataaga
    21061 aatcgtaaga actagttaat caaacgactt ataaataatg taacaccact gattaatcag
    21121 ctatatccaa aaaaacaagc attcattcag atacatcaat ctctatagaa tacaaagctt
    21181 tcattatctc aaaccattac cacaacttgg caagtcggtt aattgaagaa cacctcattc
    21241 tccacttata gcagtggagc aacaagttga gctattgttg caacagaagc taagaattct
    21301 gtactccatt taagtttttt tagactatct accgcaaacc tcagcatagt ttttttaggc
    21361 ttttctttaa tagcttcatc tttaatggtt tcgatatact cctgagcctc ttctacctct
    21421 tcttcaggta aggaatcatt caaattctcc aaaagcaaat caagctttga taataattcc
    21481 gacatatcat caatagaaac aatcatatta ttattgtggc cttgctgtat attagtgttg
    21541 taactatttc caaatatatt tactttatta tatattgaat tgttgctcat atagtcctcc
    21601 agtcgtcctg ctgcattact tgcatcttgt aacgtcacaa aaaaatctct cccatgttgg
    21661 taaattgatt caacgatttc aaattctgga tttccatatt cgcgccattc gtattcttcc
    21721 ttaaagtatt ttccgtaact atggttaaat tgcacagaat ttttaccaat tttccttaga
    21781 agttttatct ctctctcaga aataaaattt tcctcaaaag ctgattcaac tatctgaaaa
    21841 tcactaagaa atgttcctaa acagaagatt gattcattaa tagtttgttg aaaattccat
    21901 ggtattttgt tccaaaaacc attcttaatt aaagcctctt ttttttcaac aatctgtgat
    21961 tttacatttt ctgtatatat cttccaatta ttgacgtctt ccttaatgtc cgaaatcgat
    22021 tgaccttcat aagaagtccc tccaccaaaa agtccttgaa ccattttatc tcatcctctc
    22081 tataataatt actctaaatc caatacttgc tacaaatgat agcatttcgt gtggtaacat
    22141 gattgccatc gttcctgttt tatttactct gacttcaata cttggtttgt tgatgaaatt
    22201 ttaccaattg attccatcga tcgtttcatg gaatctgtat ttactatttt cattgattgt
    22261 agagattgat ttattccctc tgttgcactt ctgatacctt tagtatcaaa gttttgcatg
    22321 attgctcgct gagctgataa agctgataat gtttgaccta ttgactctat cgacctcttc
    22381 atggaatctg tatttaatat ttttattgat tgtagagatt gatttattcc ctctgttgcc
    22441 cttctgatac cttcagtatc aaagttctgc ataatttctt gctggactga taatgtttga
    22501 cctattgact ccatcgactt tttcatggaa tctgtattta atactttcat tgctgataga
    22561 gactctgaca atttagtaac acctgctaaa tcgatattac taaaataatt tccaatatat
    22621 ccttcaaaat catctgacat atcactaagg tcctcaccag caatatctcc agccaatatt
    22681 ttttgaaaag ctttctctag ttgatttgta tatctcttca atttctttat taatttttgg
    22741 tataaagcca tttctatttc tttgttatgg cttacaatat tcctaccttc tctgattaaa
    22801 ctcatgtctg tttgaatttc ttctagctta acatccatat atttactaaa tacttcactc
    22861 cacaaagaat atgaatcaga tatagtgtga ctgttatctt gaaataactt caataattga
    22921 tcctgagata tttctttata ccttacaata ttctcttcat tttcttgtgt aactaaaata
    22981 taattttctc caaagagata ttcttcaaaa tcatataaat ctatccattg taaaagattc
    23041 tttgagtcaa actttcctat tgcttgtatt tgttttcttt ttattgacga aatgatttca
    23101 tttggaattg attcctctgc ccagttatca gaaaaatatg ccggagctaa taaatagatt
    23161 aattttctta atgcccattc gtaactatgc aaatacggta atagtttttt cgaaaaaaac
    23221 aacgcatttg tatctaaaag ggtagtgtag tttaattttg atttacttgc ttcaatattg
    23281 accttagcta ggaaaatttg aaatatatgt acctctttta ttcctatctt accagtctta
    23341 ttatattgaa atactacgtg taacgaatca ttatagttct ttatgtcaaa gccaaattta
    23401 taattttttt cctgctcctg aaatcttaag atagaatcac taatatatgg tgacaacact
    23461 ttcagaaagg tttcttcgat attttccatt cttccttttt taggataata taaatattca
    23521 acaacttgct tactaatatg tcatcaactt ctttcctttc aacaatttta attttcctat
    23581 taacttgata tttgctacat aagccacaac tagcaaacag aaagagaact ttttctttac
    23641 cttgataact gtgttaatta ctggtttctt ttttctattt ctttatagta ttgttcttct
    23701 acttttaata tttttttaac ttctcttttt atgtcagcat acatagatat actgttgtcc
    23761 tttcggttga agcagtgata attctcacca tcaaactcat aaaaaagcat atcacttgag
    23821 cttccatctg aattaattaa ctgaaatttg gtattgagta gtaattcatt tttctcgtca
    23881 ttcaacttta ttaaaatagg tttgatctct tttaaataat tgaaatatat ttcggttatt
    23941 actaaattaa atttcgctac tgtataaaca taagagatac gtggggaatc tctaaattgg
    24001 ttatatatat ctttccttaa aacatctaac tcctttttta attgtccgtt aaaatcaaaa
    24061 tgaggcattg ctagaatttc atccaaatca aagtacactc tttgttgatg aatatttact
    24121 ggcagatgac catgctgaga ataatttctc atatgtaata aaaacctata cgaaaaatgc
    24181 tcatcataag gcttactcag aattttattc ttaaaagatt taaaatcttt ttctgctaaa
    24241 aagtttttca tacaaacttc aatggattca gtcaaagttt tggctgaact aattaagttt
    24301 atcgtcaaag catttatctg ataataatac tggtcttccg ttaatttttc acctgtactt
    24361 tcaattaaat cattagtaaa aattgtgaag tgtttgagca aattagttaa attatatcta
    24421 aacacattaa aaagtttatc caactctata atatgacgtg ttaattctac ataatttaaa
    24481 aatattcttt tatcagattc tgttattgtt tctaataatg aatcaatctg ccttattagc
    24541 tcgtcatctt gtaaattact gacaaaaaac ctactatcac ttctcataat tatattctcc
    24601 taatatgatt ttttcaaaat aatcaatatg taacatagaa gtatactcta ttgttacata
    24661 ccttatttct tattatatca gtatttctct gtgacacata agggtgtaac ataattattt
    24721 taaacgaaat gactacaagt agtcattgaa gcaaatctcc ataagacatt tgtaaaatct
    24781 tttaaaaaga agcgtcgaaa gaaggaactt ttcttcaacg cttcttttat tgactcaatt
    24841 ttcaatatta cttgaacgaa taatctgatt tctaaactct cgaaaaactt cttgtggttg
    24901 ataatagtct ggatctattt caaatgtttc tgagaataat tcattgaatt tttctactga
    24961 aataatgatt ttaccattct tctctcgact gttttctatt atttctgctt ctaattcaaa
    25021 taaatctagt gacgaactaa aatcataaat cctgtcatat tgaataaaaa aatcaagaaa
    25081 aagttgctca aactcttcct tatttatcac taatgtttca aacggaacaa ctttattttc
    25141 ttcagccatt cttccaattt cctcactttc attttcaaaa atcacaaaaa tgatattaac
    25201 ttaaaagcca ctaacaaaat aagacttaca aagtaaaggg taatccaaca aaataaccat
    25261 tcgctctacg tactacgatt tggtagtcct gcctttcaat cggtaattca taatagtaat
    25321 ttactgtttc ttctttgtca ctttcaatat actctggatt atctaatatc atagcatact
    25381 cataaagctc ttcaccatcc ttattttcaa tcgaccattg ttgtggacgt attggttttt
    25441 ctgtatcatt tttaagtgta taggtcactt gtaatacacg atcatgttgt gcaaattcat
    25501 ttcttttttc ggtcacttca atatttttaa atgttagttc aaccccatct gcctgataag
    25561 tttcacctat ttttaaatct attgttacaa tttttattgg cttttcttct ggaaaatgtc
    25621 tggctttaat tgttagataa cttccaaaaa gaatcaatcc tagtagcact aaaagaattc
    25681 ctttaacgag ccactgaaac gggaagtaaa ctttaaacat cttttttctc ctccgttttg
    25741 tatttgtttt ttagagaata cgataacttt tgatcaatgt catcaagctt ttcaattact
    25801 tcattactta tcattgattc tagttctgaa attttttgtt caaatttaat gctttcagaa
    25861 tcattaaatt tattatttga tggactagct aacactggta aatttgaaac cataacacga
    25921 aacatatttg ccttggaacc aaaaactcct ttgttttggt tgtaaaaatt gttgaatatt
    25981 tctttagttt cttctgaagc cgagacttta aaatatggat agttcatttt tttcttccac
    26041 ctttcttttt ttgggaggga gtctgtaggg agcttgctcc ctcagagatt tacccaccat
    26101 ctgacttgcc agaaagtagc ctagcgtgag cggggcggat tgactggcaa gtagatgggt
    26161 agctggcgta
//
LOCUS       DACGLW010000034        26138 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.034, whole genome shotgun sequence.
ACCESSION   DACGLW010000034 DACGLW010000000
VERSION     DACGLW010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26138
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.034"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            complement(128..1402)
                     /locus_tag="IXT53_001438"
     CDS             complement(128..1402)
                     /locus_tag="IXT53_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element IS1476 family transposase"
                     /protein_id="HAQ4905271.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSP
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFLRFPRKARLKVKYLV
                     MDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYR
                     RLKRYWKLLLKDSTTWEPLKRHYHRLFKRPISQTEIVDELLSYNEELRTAYHFCQLLR
                     YYFVKRGTEGFQETLKTISSTLPQSFKKKFTIFHRYQSGISNAFKVSHSNGVTEGLNN
                     KIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
     gene            1710..2516
                     /locus_tag="IXT53_001439"
     CDS             1710..2516
                     /locus_tag="IXT53_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ4905272.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            2528..3748
                     /locus_tag="IXT53_001440"
     CDS             2528..3748
                     /locus_tag="IXT53_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4905273.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     NKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            3738..5324
                     /locus_tag="IXT53_001441"
     CDS             3738..5324
                     /locus_tag="IXT53_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ4905274.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVVQTSIAQPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSEAT
                     ERMVFRFAQQPSFIEFSAEAAGEKLIFIDSNAVVKTVNDCYLFDGKEIPEFALTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENQHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKKTSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSRDEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            5363..7501
                     /locus_tag="IXT53_001442"
     CDS             5363..7501
                     /locus_tag="IXT53_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ4905275.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKIRLSGKMRGKAILDFQTAKHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKTIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRQENGHISLATNSALEMAEGEFIALLDNDDELPPF
                     ALYEVAKVLNIHPELDLIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKKLGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYPNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTAMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPSYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            7789..9180
                     /locus_tag="IXT53_001443"
     CDS             7789..9180
                     /locus_tag="IXT53_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HAQ4905276.1"
                     /translation="MNKNGEWNAARRIFIIIMDVLVLNVSIYLSFLLKFQGEVPARNL
                     ETFQHSAVFISIIFIALNILLGAYVFYSRVISDIVFITVIGQVLIALGIMVVTFAGRW
                     FAFPRGVIVLSFILGTILLSVYRIVVYKLYLRVSRDKKVLIVGLEKDVAPAIQNFQSK
                     KNNKHKVEAVVIDKYYENTKKLIDDIDIVYLASKIPEDEKINIYQLVTKKEKKLFLNT
                     TFENLTMINPNIMNFEDESIIEASGFKIQPEYELFKRLFDIIVSLILLILASPFMLIT
                     AILVKTTSPGPIIYKQVRITKNQKEFSIYKFRSMSVTAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNEENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLLLDLKILLQTIKILFDKMSSQGLDEDENIRS
                     LAEIDFSQEKIYR"
     gene            9239..10048
                     /locus_tag="IXT53_001444"
     CDS             9239..10048
                     /locus_tag="IXT53_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ4905277.1"
                     /translation="MLLSLYDKEKPEYLKEALTSIFEQTVMPKEIVLVYDGPINEKLQ
                     VVVTYFQQKDPHLFTVVKLEKNQGLGIALAIGLEHTKNRLVARMDTDDIMEPQRLEKQ
                     LEIFQKDPEIAIVGSNIEEFTGTLSNVIGRRIVPEYNDEIRVFSKRRNPFNHMTVMFD
                     KNAILEVGNYRPLIGFEDYYLWVRLLKAGYKGYNIQEILVHARAGADMYARRGGSKYL
                     LPGIKGRYRVWKDGLGSLKDFLIVICGHLVISLLPNKIRGKVYESKLRKTN"
     gene            10020..11009
                     /locus_tag="IXT53_001445"
     CDS             10020..11009
                     /locus_tag="IXT53_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ4905278.1"
                     /translation="MNQNLEKQTKLSIIIPFYNAKNTLKRAVDSVLTQGYPNIEILLI
                     DDGSTDGSSDIGDEIANLESKVACYHLENAGPSVARNCGIKEATGNLVGFLDSDDYFL
                     PDCFNNVLKDYHRNYPDIIAFGLSKGKNIENANLFVPEKKIGCSSEEAIEQMFRSKAV
                     DFYVWNKFFKRELFNHIVFPENKLYEDMVPMYEAFRAASKIDILPFSGIFYYQNNDSI
                     VYQSFNSKQYDNILQRKILVDEVKKDYPRLINLANARLVDGYLSTGFKITGKNQNQKE
                     KQKYYNQSKREIKSIYSKIRSNNEISIAKKLALFLYLINPKTYFILYKKVLKK"
     gene            11123..12118
                     /locus_tag="IXT53_001446"
     CDS             11123..12118
                     /locus_tag="IXT53_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ4905279.1"
                     /translation="MHKISVIVPAYNVEDYLENCLESISNQTHSNIEIIVVNDGSPDR
                     SGEIAEEYKKKDMRLKVIHQVNGGLSAARNSGLDSATGDFVCFIDSDDWIEPDYLELL
                     YFGTQTYGADISVLKIKKISSLEQLKEHTIAERKWEIFDREQAMKNLFINNKIGYSAN
                     NKLFNRNLFDNIRFPLGKLMEDKATTYLLIDHSEKVAVNSSAKYHYYQSPKSILRGSF
                     NPRIFDSFEVHEDILIFIDKFYPELSINVRTRYVYEAIRMMMTMIKNNYTNKEDYLRS
                     INILQRFEKEVKIDPDFSPKLKKLVKFLLINKNLPYYMSSSKIASKFLIKIESVR"
     gene            12122..13264
                     /locus_tag="IXT53_001447"
     CDS             12122..13264
                     /locus_tag="IXT53_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid biosynthesis protein F"
                     /protein_id="HAQ4905280.1"
                     /translation="MKNIKKFFSRNIFPVFIKILPIQKNLIVFSSFGGRNYSGNPRVI
                     SEKIHEKKLNYRIIWLIKKEAIITDLPDYIKRIDSDSFLAAFYLYTAKYWIDDSRKTI
                     KYAKRKKQLYFQTWHGTPLKKIEFDAKDKLPKRYLSYAKKDSESITYLLSGNRYSSKK
                     YVSAFNIVPSKILEVGTPRNDELASSKEQVFDLKKKQINVLFAPTFRNNIEDNGITQL
                     EWLGVDNLREFFKKQQQELNFMTKFHPNVHSKLATDSKSIEYMKKHQITLINDGIPLE
                     MLFEEIDVLITDYSSIFFDFALTKKPIILFNYDEKEYSLERGFYIQLSELPIISVQDS
                     YGLVDVFKNQQEKLLKSSQELLDYIGNYETGASTEKVLELIERDIK"
     gene            13261..14688
                     /locus_tag="IXT53_001448"
     CDS             13261..14688
                     /locus_tag="IXT53_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HAQ4905281.1"
                     /translation="MKKTVKDIFYNAIYQVFLIVLPLLTIPILSRRIGSTGLGIYGYV
                     FSISQFLMTVIAVGMNPFRIRNIAKSRKDKKALSLQFWNIYFIQFLIGLSVSFLYIAY
                     IYFFDVKYKNLYLIQLIFIVGLTLDISWFFQGIEEFSKVVIRNTVIKLSSVLLIIIFV
                     STKDDLWIYVFITSITNFLGSLVFWLSIHGKVGKPIFNSNVFKQLWKPAFIILTPQLF
                     MQVYTTLDKTIVGYFVDPTELSYYDQSQKIARIILAVLTSITIVMLPKISKAQAEGKS
                     ENILVYTKKSFDYTIIIALILFSVVFINTKEFVPWFFGENFKPMTINMLISCLIIVVS
                     PIGGVFANQFAIAIEKDKEYAYPMIIGAIVSIIGNVAFVPTYGAVAATIVLVVVELLV
                     FFFRVFFVRKEIKISYFFGKNVILFFIVNLTLTTWAYYFLPQLFSSSFLDMAIKSIMI
                     FIIYLPFVYFLFPDIRNEIRKITRK"
     gene            complement(14755..16044)
                     /locus_tag="IXT53_001449"
                     /pseudo
     CDS             complement(14755..16044)
                     /locus_tag="IXT53_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(16224..16634)
                     /gene="tagD"
                     /locus_tag="IXT53_001450"
     CDS             complement(16224..16634)
                     /gene="tagD"
                     /locus_tag="IXT53_001450"
                     /EC_number="2.7.7.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate cytidylyltransferase"
                     /protein_id="HAQ4905282.1"
                     /translation="MKRVITYGTFDLLHYGHINLLKRAKQYGDYLIVALSTDEFNWNA
                     KQKKCYFSYEKRKQLLEAIRYVDLVIPEENWEQKVKDIKEYHADYFVMGDDWAGQFDE
                     LEQLTDAQVIYLPRTPEISTTQIKNELNSLNSDK"
     gene            16760..18151
                     /locus_tag="IXT53_001451"
     CDS             16760..18151
                     /locus_tag="IXT53_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymerase"
                     /protein_id="HAQ4905283.1"
                     /translation="MLNEKFLKYVKGWYFIYFGFILMLRAIGPYLLLPTRYDTLLFWP
                     AGIAGVVLIIIDAIIAFREKNLKKYDWLLIAFIGAMLVSSLMNLKYGYSENIKLIMWQ
                     LIFFFMIYQFGKDYSSNFFFKVFTWILSLVWFLANLVSLYWFVIQYGIKIPIKYKYYP
                     VRLGWLGNRLFGVYTDPNFGAVISLIVIIIAIYYLVTYKHLNLLFKIFFAINIFIQFS
                     FIALSGSRTTLVCLLIATFVFSFFTSYSSRFLKTNTLKRWLTALVVGLLSIIAMYYVT
                     DITAQAWAHVPHITEIKKDISDSSGTTSSNKVDLKRPDTDGGDVSNLRFGLWKSAIEI
                     FKSKPISGGSPGYYIEYAHDKLPYTLMGKDTLTAHNFIFLVMAATGGLGLLTLLTFLF
                     IQCFKTLRYCFTQPNLVKDKHFFAILMILTIAVSALFITELILVSTIGSFIFWGYLGS
                     IQKTISKEEGVTN"
     gene            18284..19231
                     /locus_tag="IXT53_001452"
     CDS             18284..19231
                     /locus_tag="IXT53_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HAQ4905284.1"
                     /translation="MDTILITGGAGFIGSTLANYLGKENKIVVVDDLSMGKKENLDME
                     KQVTFIEGDVSDPQLMERIMKQYQFAYIFHLAAVASVADSVERPLETHRVNFDSALLL
                     LELVRKYQSGLKRLVFSSSAAVYGDEPTLPKKEESVIRPLTPYAIDKFASEQYVLDYC
                     HLYDVPTSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQLAGEKTEFTLFGDGSQSR
                     DFVYIEDVIQALLLVAKEEKALGQQFNVGTGKSTTLLELIHSIDQILGTELALKYEAE
                     RSGDIRDSLADISKIRSLGYQPKFDILNGMERYLKTEID"
     gene            complement(19271..20431)
                     /locus_tag="IXT53_001453"
     CDS             complement(19271..20431)
                     /locus_tag="IXT53_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAQ4905285.1"
                     /translation="MAKGKHQKKQQISLSFLAVLLLAVSFFSTGIITGWQLTKQPQPQ
                     IYKTEPAQLASNLTSNEQMLASKIDQLLQKSDYIGSIYVRKNNRVILEKGYGYANLRI
                     KESNSPSLYYQIGSIQKAMTALLILKQIQSGKITFDTKLSEFYPQIPGSQQISIKNML
                     YMRSGLHRTASPKKPMSDSEIIQFALHHLKFTGYDTYHYEPLNYTLLTGVLIKLMNQP
                     YETLLKKEIIRPLHLEHTDFYENVKNSPQHAVSYQMSATDDYSKALVEPETDIRNELG
                     TGNISMSVYDLNKFFTSVLSGDIIPKELLFSLWQNNGDKHPYSGGVYSGNDYILAQGN
                     INRFHAVAAMKKDTKDAIVMESNIQSDKNIKLPATDLRNQIYELMEGVNLKS"
     gene            20596..21771
                     /gene="mltG"
                     /locus_tag="IXT53_001454"
     CDS             20596..21771
                     /gene="mltG"
                     /locus_tag="IXT53_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HAQ4905286.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPNGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELYQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEKAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVQKYVNNS"
     gene            21907..22386
                     /gene="greA"
                     /locus_tag="IXT53_001455"
     CDS             21907..22386
                     /gene="greA"
                     /locus_tag="IXT53_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HAQ4905287.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGD
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            22554..23285
                     /locus_tag="IXT53_001456"
     CDS             22554..23285
                     /locus_tag="IXT53_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HAQ4905288.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKYPRTKFQNFQLILGSLVIFFSLVNSPALWMMVVLAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMVQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAIISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKICSS
                     DYDENPRRLKIITNTLVGDVEVIRI"
     gene            23282..24349
                     /locus_tag="IXT53_001457"
     CDS             23282..24349
                     /locus_tag="IXT53_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ4905289.1"
                     /translation="MMGRISKAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLMAISLGVGLIVFLLLSLIQKAKYGKIEEKLRALSSGNYESKLLHQP
                     IPSASDDLYIKDIDMEIAKIKEKMIEVSSELQIMTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLTSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            24356..24988
                     /locus_tag="IXT53_001458"
     CDS             24356..24988
                     /locus_tag="IXT53_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ4905290.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     ALRPDVILMDLVMEEMDGIESTKAILKDCPKAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNDPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            25128..25793
                     /locus_tag="IXT53_001459"
     CDS             25128..25793
                     /locus_tag="IXT53_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HAQ4905291.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            25821..26114
                     /locus_tag="IXT53_001460"
     CDS             25821..26114
                     /locus_tag="IXT53_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HAQ4905292.1"
                     /translation="MELTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
BASE COUNT         8830 a         3945 c         5092 g         8271 t
ORIGIN      
        1 aaaatacgag agaggctgtg acaaccgtcg cagccttttt tggctcttcg tcaaatcgtg
       61 ttggtgaaga caaatttgaa taaaacttag tccgatatgc ggcgcgttct gtgcgccgca
      121 tattttttta gttttcaaag atcagacctt gttgtaaata gatccgtgcc cgaaagttat
      181 aaaagttgcg ataaccaaac gcaattcgtt taatcagctt gatcttgtta ttcagcccct
      241 cagtgacgcc gtttgagtgg gacaccttaa aggcatttga aatacccgat tggtagcgat
      301 ggaagatcgt gaatttcttt ttaaacgact gaggtaacgt agatgaaatt gttttaaggg
      361 tttcttgaaa gccttccgta cctcgtttaa cgaaataata gcgtaataat tgacaaaaat
      421 gatacgcagt tcgcaattct tcgttgtagc ttaacagctc atcaacgatt tctgtttgac
      481 taattggtcg tttaaacagt cgatgataat gccgtttgag tggctcccat gtcgtggaat
      541 ctttgagtaa gagtttccaa tatcgcttga gccgacggta cttcttctga ttttcagagt
      601 gtgaggttcg aaaacgattc atgatctgga cacgtaattg attgaagctg cgattgatgt
      661 gttggacaat atgaaagcgg tcagttacga tctccgcatt tggaaagact gttttaagca
      721 actgacaata gctggcattc atgtccatca ctaggtactt gacctttaaa cgggcttttc
      781 taggaaagcg caggaagtat tgagttagtt tgtaaagtct gcgatcttca aggatcgtaa
      841 atagctgatg ggttgtccca tcaacacaaa taaaactcat ggaaccagaa acagacttca
      901 ttgatttgaa ctcatccata cacaacaccg tcggaaggaa gttcatacgt ggctggtaat
      961 gcttcgttgc ttcacgcaag atccgtgtca ccgtcccatc cgagacgaag tacttcttag
     1021 cgattagttt acgtgattga ttttcgtaca gttccatgag aatcgcctgc ttcagttcct
     1081 ttgttagttg atgatgttca tcaactaagt ccgttttcgc agaaaaggtc cttccacaat
     1141 catggcagag aaaacgggta cgttttaatt ctaaaatagt taacttttct ttgactttca
     1201 acatctgagt gcgtgtcgta taggaaccgt tgcggataat acgaaccgat cgacagtgtt
     1261 cacaatagga aggagagtca tccaattttc ctttaataaa ctgcgcttga acgcctttat
     1321 attctttttc aatcagccat tcttcatcaa aaaataaatg tttatctgtt aatccgagta
     1381 attttctagt atgattatcc attgagagac tcctttggta ggatgacgtt ttggtcgact
     1441 aaattttacc aaaagtgagt ctctttttga gtttaaacga agaaaaagtg ttagtagaaa
     1501 atcgccttcg attttccacc aacacaaaat attatagaac cccttttttg gtttattgca
     1561 attgtctagc atcttctgta aaatagaaac gttagagaaa atcttgtatc atagaaaatt
     1621 aagaaagaaa taagaacgca gctcggtacc aaaaatatgg taaaatggca cttgactgta
     1681 attcactgaa atcgcttgga ggaacacaaa tgtttaaaga tttgtattta tttatgaaag
     1741 acatttatca gaatagaaaa ttactgctac agttttcgat caatgacttc aaatcaagat
     1801 atgcaggatc gtttttagga atcttatggg cgtttatcaa tccgctattt actgtattga
     1861 tttattggct ggtatttggt gttggactga aagcacaaat gatcgatgca aaatacccat
     1921 ttatcgtata tttagttacc gggatgattc cctggttctt cttctcagat gcatttgcat
     1981 caactacggt tgtttttcgg gaatatactt atttagtaaa gaaagtcgta ttcaatattc
     2041 gtatcttgcc cacgacgaaa atcctatcca atctgtacac ccatttattc ttcattctga
     2101 ttggcatagc tgtcgcgctt ataaatggta tatatccttc agcaatgtct ttacagctga
     2161 tttattattt attttgcatg tgtgctttct tgacaggatt gacttggctg acggcatcga
     2221 tccaaccatt tttacctgat atcatgcaat taattacgat cgtgctgcag ttgatcatgt
     2281 ggacattacc gatattatgg agccctaatc agtttgattc aggtcttgta agcatattga
     2341 aattaaatcc gctgtactat gttgttcaag gttatcgtga gtcattttta agtgaagctt
     2401 ggttctggga gcatggaaca tatactttgt atttctgggt tttcacgtta gtaatgttcg
     2461 taatcggctc tgcagtgttc cgtagattga aaccacattt ttcagatgtg ctgtaaggag
     2521 aatagctatg acagaatatg caattagact aaaagatatc acaaaatctt ataatatgta
     2581 cgcaaaacct tcggatcgat ttcgtgaagc cctcaatcct tttaagaaat cctatcatga
     2641 tgtattttat gcgttgaaaa acgtgaatgt agaaataaaa aaaggtgaga tgatcggttt
     2701 cgtgggagaa aacggatcgg gaaaatctac aatgttgaaa atcattacgg gagtactgac
     2761 accaacatcc ggcacgatgg aaatcgaagg aaaaatctca gctttgctag aactaggatc
     2821 aggattcaat cctgagtatt ctggctatga aaatatctat ttaaatggaa tggttctagg
     2881 attttcaaga gaagaagtcg atgaaatggt cgatgatatc attgaatttg ctgatattgg
     2941 cgaccatatt tatcaacctg taaaaaccta ttcaagtggg atgttcgttc gtctagcttt
     3001 tgctgtagcg atcaatgtga atcctgatat tttgattgtg gatgaagcat tggcagtagg
     3061 agatcttgaa ttccaactga aatgtatgga aaaatttaca gaaattaaga actcgggaaa
     3121 aacgatttta ttcgtttctc atgatattag ttcgattcgc cgtttttgtg atcgttccta
     3181 ttggttgaaa aatggagaag ttgtcgaata tggtgatacg atggaagtaa cgacgaatta
     3241 cgaaaacttt ttgaagaaaa aatcagttaa aacagtagat cgaaataaaa accaagtaga
     3301 acgcttcgca gctccagata tcgtagacgt gacgaaagcc gaattgttgg ataaaaataa
     3361 aaatccagtg gatatgctgg aacaaggaga aaaactatac gttaaagtaa cctatgaagt
     3421 aaaagacgat acaatcaaaa atccagtttt aggaattgct ctgcgtacag tcgacaattt
     3481 ttatgtgtgc ggactcaata ctctactaga tggtatcaag atcccttgga aaaaaggtga
     3541 gaatatcttt tatctggagt atgaaaagtt aggtttgtta gctggtgaat attattttga
     3601 cgttgcggtc tttgaagaaa atgcaacggt tcctttggta tacaagacaa aatacatgaa
     3661 cttgtttgtc agcggttctt atatcggaga aggcatcgtg gtcttagacc ataaatggga
     3721 agaagggact catagcaatg aaatatgatt ttgaaatgga cctagatgaa caaagcagtg
     3781 tgggaaaaat tgccgcacag atcaaaccag gaagtaaagt tttagagttt ggtcccggaa
     3841 acggtcgatt aacgaaacat ttgatcggag caaaacaatg tgaagtgagc attgttgagc
     3901 tagataagga attattcgat tttgtcagcg aatttgccca agatggattt tatggagata
     3961 tcgaaagttt tgaatgggca aactattatg ctggacagac atttgattac gtcttgtttg
     4021 cggatgtttt agaacattta gtagatcctg gaaaaacatt gaagaaagtc agagaatttt
     4081 tgaatgaaga aggagagatc ctgattacat tccctaactt agcacataat tctgtcatga
     4141 tcgatttgtt caataatcaa ttgccttggg cttcttatgg tctcctagat gaaacccata
     4201 attcttttta tactcatgat ggtttccaaa aagtttttga aaaagccgga ttattcatta
     4261 atattgaaga ttacttgtac ttggctgtag gagatacaga attaaaatca acttatgaag
     4321 agttgccgga agctgtacgc tatgatttca aaatgcgacc atttggtgaa gtctaccaat
     4381 actttttctc attgatgaaa catccagtag tacaaacttc cattgcacag ccacaaaact
     4441 ctaattatgt aaaagtacta gaagtaaccc agcaaacgaa acaagatgaa acaatccaac
     4501 aaataccatt caataatttc acaggggaaa atgagacact aagtttcccc gtgtcagaag
     4561 caacagaaag aatggtgttt cgcttcgcac agcaacctag ctttatcgaa ttcagtgcag
     4621 aagctgctgg agaaaaactc atatttatcg attcaaatgc tgtagtcaaa acagtaaatg
     4681 attgttattt gtttgatggg aaagaaatac cagagttcgc tctaacggat atttctggga
     4741 aagaagtgac tatccattgt cactatcgct ttatcgggga attgacacca acaatgaaag
     4801 aattgctgga aacaattcgt cctatggctg aagtcacaaa gcagctttct gagaaaaatg
     4861 atgaattaga aagagaaaat cagcatcttc ttgaggaaaa tacaagacta gaccatacgt
     4921 taaaaacaac cacaaatcgc tattgcacat tgttggaaag tgatgaatgg acgatcaaac
     4981 atagactagg acgacgtaag aaaaaaacat cgaaaaaaat acaagaaaaa gagctttcta
     5041 tatgtattga tgagaaaata tgggatgcag aaacaaaaat attaaaaatc atcggatggg
     5101 gaatcgctaa ttcggatcgc cagccattgt cctataagtt atcagctgac caatcgccat
     5161 ttttcgaagc aattcctgtt tcacgagatg aagtcaatca agcagaacag ctcgcaaaag
     5221 gaacagaagc gggatttgaa ttacgaatcc tttgcgagca agaacgttca ttcttagtgg
     5281 aagcagtagc tcaaaatggt caatcatgga tcattgaaat gtaaataatg attaacttat
     5341 aaagataagg agtaaacgaa tcgtgcaaaa aaaagaaatt gccgtattta tcgatcaaat
     5401 cactcgtgaa cgggccactg gcgatttgct tattgtgggc tgggcaattg atgaagtgac
     5461 caaagaaatc cctacgataa aggtagaaaa agaaaatgtg attgctgaag caacacatgt
     5521 cgtaagattg gatatcaatc atttatataa tcttgatgtt aagacccaaa gcggttttaa
     5581 gatacgttta tccgggaaaa tgagaggaaa agcgatctta gattttcaaa cggcaaaaca
     5641 tcaaaatggc atagctgtga aactaaatgg taaataccct tatgatgacg gcattgaatc
     5701 ttcatgggaa agaaaaaaaa gattactgaa aaaagggata aattatgcaa ggactcatgg
     5761 cgtaaaaaaa acaatccgcc gggtcaaact ggaattgaaa ccaggatcga tcgactatgc
     5821 agagtggatc agtcgtcacg agaaacctga tctgaaagag caaaggaaag aagcacaagg
     5881 gtttgcatac cgtccattga tttccatact tatgcctgtc tataacgttg aaatcaaatg
     5941 gctcgaaaaa tgcattgatt cggtattgga tcaaacgtat gatcactggg aactatgtat
     6001 cagtgatgat gcttctactg atccagctat ccgaaagtgt ctggaatcct atcaagcaaa
     6061 ggatgataga atcaaagttg ttttccgaca agaaaatgga catatcagtc tggcaactaa
     6121 ttctgcgttg gaaatggcag aaggagaatt tatagcgctc ttagataatg atgatgaatt
     6181 accgccattt gcattatatg aagtggcaaa agtgctaaac atacatccag aactggattt
     6241 gatctacagt gacgaagata aaatcgatgc agatggcaat cgctttgatc cgcacttcaa
     6301 agcagactgg tctcccgata cattgatggg aaataattat atctctcact taggcgtata
     6361 tcgaacaagt attgtgaaaa agcttggcgg tttccgtaaa ggctacgaag gctctcaaga
     6421 ttatgatttg gtccttcgtg tgacagaaca aatccctgca gatcatattt accatattga
     6481 ccgagtcttg tatcattggc ggacgatccc aggatcaacc gcaagtaatg gtgaagccaa
     6541 aagttatatc tatgattctg gagtgaaagc attgacagat gccctgtcta gaaggaatat
     6601 caaaggaagc gtacgtcctg gcagaatttc tggattttat gagattgctt atgacgtctt
     6661 gcaagaggat ttggtcagcg tgatcattcc aacgaagaat ggctatgaag atctaaaaac
     6721 ttgtgtggat tcgattatcg aaaaaacgtc ttatccaaat tatgaaatca ttattgcaga
     6781 taatggaagt acagatccta aaatgcagga attatttgca gaatacaagc atcagctgaa
     6841 agaccgattt atcgttgaat taattgacat tccattcaat tattcacgta tcaacaatct
     6901 ggcagcagaa aaagcaaacg gcaaatactt tttattcttg aataacgata cagaagtgat
     6961 tgaacctgac tggatgacag cgatggtttc ttatgcacag tttgatcgaa tcggctgtgt
     7021 aggagcaaaa ctgttttatc cagatgatac aacgcagcat gcaggagtat tattaggaat
     7081 cggtggtgtt gcaggacatg cattgaataa ctatgatcga acacattgtg gttatttcgg
     7141 ccgattggtc attgacgtca attacttagc agtcactgca gcttgtatga tggtcaaggc
     7201 agctgacttc aatgctgtca atggatttga tgaaacatta gaagtagcat tcaatgatgt
     7261 tgacttgtgt ttgaaagttt atgaactggg tcgttacaat gtctatgcac atcaagcaga
     7321 attgtatcat ttcgaatcta aatcaagagg ttatgaagat acaccagaaa aacaaagacg
     7381 ttttgccggg gaaataaaaa aaatgcaaga taagtggcct tcttatattg ctcatgaccc
     7441 attttataac gataatctaa ctaaagaagg aatcggcgat ttttcattga gacctgattg
     7501 attaaaagaa ctgtattttc atttttattg atgaaaatac agttttttta gtatatgaaa
     7561 attgtaactt gtgaccgaag agatgatagg aagaacaccc attcagtagg aagaatgtga
     7621 tacgattttt gtccaactct ctgttaggca gaaatataca tataaacata caatagtcac
     7681 cttttctagt ttcttttaat ttattttaca atttggttat tcactaggtt tttaaacttt
     7741 gctatggtaa gatgatagag gcattatttt atggttagga gtgtatatat gaataaaaat
     7801 ggggagtgga atgcggcacg taggatattc attatcatca tggatgtact tgtattgaat
     7861 gtgtcgatct atcttagctt tttgctgaag ttccaaggag aagtcccagc aagaaactta
     7921 gagacctttc agcattcagc agtttttatt tccattatct ttattgcgtt gaatattttg
     7981 ttaggagctt atgtattcta tagccgagtg atcagtgata tcgtcttcat tactgttatt
     8041 ggacaggtgc tgatagcatt ggggatcatg gtagttactt ttgctggacg ttggtttgca
     8101 tttccgcgtg gcgtcatcgt gctctcgttt atacttggca cgatattact tagcgtttat
     8161 cggatagttg tttataaact atacttaaga gtcagtcgtg ataagaaagt ccttatcgta
     8221 ggtttggaga aagatgtcgc accagctatc caaaactttc aatctaagaa aaacaacaaa
     8281 cataaagttg aagcagtagt gatcgacaag tattacgaaa acacaaaaaa attgattgat
     8341 gatatcgata ttgtctactt ggcttctaaa atcccagaag acgaaaaaat caatatttat
     8401 caattagtaa ctaaaaaaga gaagaagcta tttttaaata caacttttga aaatttaacg
     8461 atgatcaatc cgaacatcat gaatttcgaa gatgaaagta tcattgaagc ttccggtttc
     8521 aaaattcagc cggaatatga attattcaaa cgtctttttg atatcatcgt ttcgcttatt
     8581 ttactgattc ttgcttcacc ttttatgcta atcacagcga ttttggtaaa aacaacttca
     8641 cctggaccaa ttatttataa acaagtacgt attacgaaaa atcaaaagga attttcaatc
     8701 tataaatttc gttccatgag tgtaacagct gaagcaaaat caggaccggt cttggcaaag
     8761 agcaatgatg cacgtgtgac accagtaggc aaatttatcc gtgccgtacg ttttgacgaa
     8821 ttacctcaaa tctttaatgt attgaaaggc gatatgtcga tcgtcggacc acgtccagaa
     8881 agaccatttt tcgttgatca gttcaatgaa gaaaatcctt attattattt aagacacaat
     8941 gtacgtgcag ggattaccgg atatgcacaa gtatacggaa aatatgtatc agattataat
     9001 agtaaactac gttttgattt gttgtatatc aaaaagtatt ccttactatt agatttgaag
     9061 attttacttc aaacaatcaa aatcttattt gataagatgt cctcacaagg actagatgaa
     9121 gatgaaaata tccgttcgtt agcagaaatc gacttttcac aagagaaaat ttatcgctag
     9181 atgggaaaaa ttttgagcaa agaagtagga gagaacattg gaaaataaaa atataagtat
     9241 gctgctttct ttatatgata aagaaaaacc agagtattta aaggaagcat taactagtat
     9301 ttttgagcaa acagtaatgc caaaagaaat tgtattagtt tatgacggcc ctataaatga
     9361 aaaacttcaa gtagttgtca cttatttcca acaaaaagat cctcacttgt ttacagttgt
     9421 aaaattagaa aaaaatcaag gattggggat tgctttagca attggattag aacatacgaa
     9481 aaatcgctta gtagccagaa tggatacaga tgatatcatg gaaccccaac gattagaaaa
     9541 acaattagag atcttccaaa aagatccaga aatagcgatt gttgggagta atattgagga
     9601 atttactggc acgcttagca atgttatcgg aagaaggatt gtacctgaat ataatgatga
     9661 aatccgtgtg ttttcgaaaa gaagaaatcc ctttaatcat atgacagtca tgtttgataa
     9721 aaatgctatt ttagaagtgg gaaactatcg cccattaatt ggatttgaag attattattt
     9781 gtgggtaaga ctgttgaagg cgggatataa agggtataat atccaagaga ttctagtcca
     9841 tgctagagca ggtgctgaca tgtatgctag acgtggagga agtaaatatc ttttaccagg
     9901 aattaaaggc cgatatcgcg tatggaaaga tggtctagga tcactaaaag attttctgat
     9961 agttatttgc ggacatcttg taattagttt attaccgaat aaaattaggg gaaaagtata
    10021 tgaatcaaaa cttagaaaaa caaactaaac tttctataat catacctttt tacaatgcaa
    10081 aaaatacttt aaaaagagct gtagacagcg tcttaacgca aggttatccc aatattgaaa
    10141 tcttgttaat tgatgatgga tctacagatg gtagtagtga cattggtgac gaaattgcta
    10201 atctagagag taaagtagca tgttatcact tggaaaacgc tggtccatca gttgctagga
    10261 attgtgggat aaaagaagca actggaaatt tggtaggatt tttagatagt gacgattatt
    10321 ttttacctga ttgttttaat aatgttttga aagactacca taggaattac cccgatatca
    10381 ttgcttttgg attaagtaaa ggaaaaaata tagaaaatgc aaatttattt gttcccgaaa
    10441 agaagattgg ttgttcaagt gaagaagcaa ttgagcaaat gtttagaagt aaggcagtgg
    10501 atttttacgt atggaataaa ttttttaaaa gggaattatt caatcatata gtttttccag
    10561 aaaataaatt atatgaggat atggttccta tgtatgaagc ttttagagcg gctagtaaaa
    10621 ttgatatttt gccattttca ggtatttttt attatcaaaa taatgatagc atcgtttatc
    10681 agtcttttaa ttcaaaacag tatgataata ttttgcaaag aaaaatatta gttgatgaag
    10741 taaagaaaga ttatcctcga cttattaatt tggccaatgc tcgattagta gacggatacc
    10801 tttcaacagg gtttaaaatt actggcaaga atcaaaatca gaaagaaaag caaaaatatt
    10861 ataatcagtc taaaagagaa ataaaaagta tatattcaaa aataagaagt aacaatgaga
    10921 tatcaattgc taaaaaatta gctttatttt tatacttaat taatcctaaa acttatttta
    10981 ttttatataa aaaagttcta aaaaaataaa gtttttttcg atatgttatt ttttatgttt
    11041 attaacttcg gaagactaaa attgttactg gtaatgatac atttgtaaaa ataaaaaaga
    11101 tgatttttag gaagtgaaga gagtgcataa aattagtgtt atagtacccg catataacgt
    11161 agaagactat ttagaaaact gcttagaatc tatttccaac cagacacatt caaatattga
    11221 aataatagta gtcaatgatg gttccccgga tcgaagtggg gaaattgcag aagaatataa
    11281 aaagaaagat atgcgattaa aagtgataca tcaagtgaat ggtggtttaa gtgcggctag
    11341 gaattctggt cttgattctg caacaggaga ttttgtgtgc tttattgaca gcgatgattg
    11401 gatagaacca gactatcttg agttactata ttttggcacg caaacttatg gagcagatat
    11461 ctcggtgcta aaaataaaaa agatttcaag tttagaacaa ctaaaagaac atacaattgc
    11521 tgagagaaaa tgggaaatat ttgatagaga acaagcaatg aaaaacttat ttataaataa
    11581 taaaattggc tattctgcaa ataataaatt attcaataga aatttatttg ataatataag
    11641 attcccactg ggtaaattaa tggaagataa ggctactact tatttgctga tcgatcattc
    11701 cgaaaaagtt gctgtcaatt catctgctaa atatcactat taccaaagcc ctaaaagtat
    11761 actgagagga agcttcaatc ctagaatttt tgattctttt gaagtccatg aagatatact
    11821 aatattcata gataagtttt atccggaatt atcaattaat gttagaactc gctatgtcta
    11881 tgaagcaata aggatgatga tgacaatgat taaaaataac tataccaata aagaagatta
    11941 tttacgttct ataaatattt tacaacgctt tgaaaaagaa gtgaaaattg atcccgactt
    12001 ttcaccaaaa ttaaaaaaat tagtaaagtt tttattaata aataaaaact tgccttacta
    12061 tatgtcaagt agtaagatag caagtaaatt cttgattaag atagaatctg tgaggtaaga
    12121 gatgaagaat ataaaaaaat ttttttcaag gaatattttc ccggttttta ttaaaatatt
    12181 acctattcaa aaaaatttaa ttgtattttc aagttttggc gggcgtaact attctggaaa
    12241 tccaagagtt atttcagaga aaattcatga aaaaaaattg aattacagaa ttatttggtt
    12301 aatcaaaaaa gaagcgatta taactgattt accagattat attaaaagaa ttgactccga
    12361 ttctttttta gctgcctttt atttatatac agctaagtat tggatagacg atagtcggaa
    12421 aacgattaag tatgcaaaaa ggaaaaaaca actttatttt caaacatggc acggaacgcc
    12481 tttaaaaaaa attgaatttg atgcaaaaga taagttacct aaacgttacc taagctatgc
    12541 gaaaaaagat agtgagtcaa ttacatattt attatctggt aatcgctata gttctaagaa
    12601 gtatgtttca gcattcaata tcgttccttc taaaatatta gaagtaggaa ctcctagaaa
    12661 cgatgaatta gcctctagta aagagcaagt gtttgacttg aaaaagaaac aaatcaatgt
    12721 cttatttgct ccaacttttc gaaataatat tgaggacaac ggaattacgc aactagaatg
    12781 gttaggtgtg gataacttac gtgaattttt taaaaagcaa caacaagaat taaattttat
    12841 gactaaattt catcctaatg ttcatagtaa attagcaact gattctaaaa gtatagaata
    12901 tatgaaaaaa catcaaatta ctttaattaa tgatgggatt cctcttgaaa tgctttttga
    12961 agagatagat gttttaatca cagattattc atctattttt ttcgattttg cgctaacaaa
    13021 aaaaccaatt attcttttta actatgatga aaaagaatat agcttagaaa gaggttttta
    13081 tatacagtta agtgagttac caattataag cgtacaagat agttatggcc ttgttgatgt
    13141 ttttaaaaac caacaagaaa agcttttgaa aagttctcaa gagttacttg attatattgg
    13201 aaattatgaa acaggagcaa gtacagaaaa ggttcttgag ttgatagaga gggatataaa
    13261 gtgaaaaaaa cagtaaaaga tatattttat aatgcgatat atcaagtctt tttgattgtt
    13321 ttacccttac ttactatacc tatacttagt cgtagaattg gttcaactgg tttagggatt
    13381 tatgggtatg ttttctcaat atcacagttt ttaatgactg tcattgctgt gggaatgaat
    13441 ccttttcgta ttcgaaatat cgctaaatca aggaaagata aaaaagcact atctttacaa
    13501 ttctggaata tttactttat tcaattttta ataggcttga gtgtttcctt tctctatata
    13561 gcctatattt atttttttga tgtaaagtat aaaaatttat acttaattca attgatattt
    13621 attgtagggc taacattaga tatttcttgg ttttttcaag gaattgaaga attttcgaaa
    13681 gtggttatta gaaatacagt tatcaaatta tcatcggtat tgttaataat catttttgtt
    13741 tcaactaaag atgatttgtg gatttacgtg ttcattactt caattactaa ctttttagga
    13801 agtttagttt tctggttaag tatccacgga aaagtaggta aaccaatctt taactctaat
    13861 gtttttaaac agttgtggaa accagctttt attattttga ctcctcagtt gtttatgcaa
    13921 gtgtacacaa ctttagacaa aacgatagtt gggtattttg tggatccaac ggaattatct
    13981 tattatgatc agtctcaaaa aattgctcgt attatattgg cagtcttaac atcaattact
    14041 attgttatgt tgcctaaaat atctaaagca caggcagagg ggaaatcaga aaatatttta
    14101 gtttacacaa aaaaatcttt tgattacacg attatcattg cattaattct ttttagtgtg
    14161 gtgtttatca atacaaaaga atttgttcca tggttttttg gggagaattt caaaccgatg
    14221 acaataaaca tgcttatttc ttgtctaatt atagttgtta gtccaattgg tggagttttt
    14281 gctaaccaat ttgcaattgc tatagaaaaa gataaggagt atgcttatcc aatgatcatt
    14341 ggtgcgattg taagtattat tggaaatgta gcttttgtgc ctacttatgg cgctgtagcg
    14401 gctacgatcg tactcgtggt agtagaatta ttagtctttt tctttagagt attttttgta
    14461 cgaaaagaaa taaaaatatc ctatttcttt gggaagaacg ttatcttatt ttttatagtc
    14521 aatcttactt tgactacttg ggcctattac tttcttccac aattattctc ttcttcattt
    14581 ttagatatgg caattaaatc aataatgatt ttcattattt atcttccgtt tgtttatttt
    14641 ctttttccag atatacgaaa tgaaataaga aagattactc gaaaataaaa taatgttgta
    14701 aaaaagtagt tctttgtcaa atcgggctga taaggtcatt tatgaacaac aaagttagta
    14761 agagttacta gcttttaatg catagctgat taacctgttg attagaaaaa tccacaagcg
    14821 catattggta aaactgcgaa acccataagc aaatcgtttc aatgttttga tatgtgtgcg
    14881 aagattctct aattttgtta ttagaatact ttttcgaaaa tgctagttaa aagccttttt
    14941 cagatatgaa aaaggctttt tgagatagtg aagtttcgag ggaacttctt tggtcgtttc
    15001 tcgaagtagc tagctgtttc ttgtgataat tgctaagcag cttttgataa actgcttagc
    15061 aattttaagt tcataagaga ttgagggtag acgatcaact acttcacttt ctgttaaata
    15121 gcgccattga aaattctgcc atttcttgaa tgttgtgtaa tccaactcgt catctgattt
    15181 tagaagtaat ttatacaaag gtttcaattg tcgatagtac ttagcttgtg aagtgtcttt
    15241 ttgattcaac tgcttcatca cacgttttcg aacacgatcg aaaaacttgt tcaagtgttg
    15301 tccaatgtga aagcaatcaa tgatgatttg ggcgtttgga aaggactctt tgactaatga
    15361 aaaataaggg gcattcatat ttgtgacaat gtatcgaacc cccatatgag cttttcttgt
    15421 aaaacgcatg aaataagcaa tcaaatcttt ttttctccgg ctggacaaaa tgtcaaagag
    15481 cttccctgtc tctctattca tacaagaaaa ggagaacttt ccatgggaaa ttgaaagaga
    15541 gcgaaactcg tcgaatagta aacaatgagg taaatagtct agcatctgtc gcagcattgc
    15601 cgatagagca gataatgtcc gttgaaatgt cgttactgaa agatcacaac gtttcgtaag
    15661 tgtctttaac gcttgacgct cggtcaactc ttctaatatc gtgtaatgaa cttgtttcga
    15721 aataaaacat gaaggaacga caagatacgt atgagcagta aaatattttc cgcatgattg
    15781 gtagtggtaa tgttgttttc taagacgcaa ataggtttat ttattcccac tgcgatttaa
    15841 gataatcatt gaaatcttct tgccgttttt cacgagctgc ttcgcagttt ctttacaaaa
    15901 tagacaatgt ctaaaagtgc gaactaatgt accttcgatt acgatggctt tcttgtcctt
    15961 ataaaaagtt ttttgaaaag aattaatttc aatattttta tctttaatag tgaagtgctc
    16021 tttgatagaa cgagaaatgg atatacaaaa tcttcctttc agattgatgt tttagacgac
    16081 tctattttat cgaaattttt gtatgtctgt tttttttaca agaaaatcta ggatcgacag
    16141 aaaattctat cagtcctaga aattatagag ccattttttt acgtcttatc cagatatatt
    16201 gttctttcat tgtttaaaaa atattattta tcactattta aagaattaag ttcgttcttt
    16261 atttgagttg tagagatctc aggtgttctt ggtaaataaa ttacttgtgc atctgttagt
    16321 tgttcaagtt catcaaattg tccagcccaa tcatcgccca tcacgaaata atctgcatgg
    16381 tattctttta tatcttttac tttttgttcc caattttctt caggaataac taaatcaacg
    16441 tagcgaatag cttcaagtaa ctgttttctt ttttcataac taaaataaca ttttttttgt
    16501 ttcgcattcc aattgaattc atctgtagaa agggcaacaa taagataatc tccatattgt
    16561 tttgctcgtt tcagtaaatt gatatgcccg tagtgaagca aatcaaaggt accgtaagta
    16621 attactcttt tcatctttca cctctcgaaa ttcattaata tacattgtta gtttagcatt
    16681 tttttgttag aattgaaaga accagtttat cctaaaaagg ataagaaatg tttatgagaa
    16741 ataggtggat ggaggaaaaa tgttgaatga aaaattttta aaatatgtga aaggttggta
    16801 ttttatttat tttggtttta ttctaatgtt acgagcaata ggcccgtatt tactattacc
    16861 tactagatat gatactttac ttttttggcc tgctggaata gcgggagttg tcttgatcat
    16921 tatagatgca attattgcat ttagagaaaa aaatcttaaa aaatatgatt ggctattaat
    16981 tgcttttatt ggcgcgatgc tagtaagcag tttaatgaat ttaaaatatg gatatagcga
    17041 aaatatcaag ttaatcatgt ggcaattaat ctttttcttt atgatttatc agtttggcaa
    17101 agactacagt agtaactttt ttttcaaagt atttacttgg atactttcat tggtatggtt
    17161 tttagctaat ttagtatcat tatactggtt tgtcattcaa tatggcataa aaattccaat
    17221 aaaatataag tattacccag ttcgtttagg gtggttaggc aaccgtttat ttggtgtata
    17281 tacagatcca aattttggtg cggttatatc attaattgtc atcattattg ctatatatta
    17341 tttggtcact tacaagcatt taaatcttct atttaaaata ttttttgcaa ttaatatttt
    17401 tattcaattc tcgtttattg ctttatcggg ctcacgcacc acattagttt gtttattaat
    17461 agcaactttt gtttttagtt tctttacaag ttatagtagt cgttttctta aaactaatac
    17521 tttaaaaaga tggctaactg cattagtagt aggattgcta agtataattg cgatgtatta
    17581 tgtaacagat ataactgctc aagcatgggc acatgttccg catattacag aaataaaaaa
    17641 agatatatct gattcatcag gaacaactag cagcaataaa gttgatttaa aacgccctga
    17701 cactgatggc ggggatgttt ctaatttaag gtttggactt tggaaaagtg caattgaaat
    17761 attcaagagt aagccgatat ccggcggttc tccaggatac tatattgaat acgcacatga
    17821 taaactacca tatacattaa tgggaaaaga tactttaact gctcataatt ttattttctt
    17881 agttatggct gctacaggag gattaggtct attgacattg ttaaccttct tatttatcca
    17941 atgctttaaa actttgcgtt attgtttcac acaaccaaat ctagtaaaag ataagcactt
    18001 ttttgctatt ttaatgattc ttacaatagc agtttcggca ttatttatca cagaactcat
    18061 tttggttagt acgataggtt catttatttt ttggggatat ctgggttcta tccaaaaaac
    18121 tatatctaaa gaagaaggag taactaattg aaactatttt taggtatata aagtagaaaa
    18181 gaaaactagt tatcccaact tcaacaaatt ttatagtttt tacaatataa aaatggtaaa
    18241 ctaactttat taagtatgag aatgcataat tggagtgaat ttgatggata ctatattgat
    18301 tactggtgga gctggtttta tcggttcaac gctagcgaat tatttgggaa aagaaaacaa
    18361 aatcgtagta gtcgacgatt tatcaatggg aaaaaaagaa aacttggata tggagaaaca
    18421 agtgactttc attgaggggg atgtatcaga tcctcaattg atggaaagaa tcatgaaaca
    18481 gtatcagttt gcctatattt ttcatttagc tgctgtagct agtgtggcag attctgtaga
    18541 acgtcctcta gaaactcatc gtgtaaattt tgacagtgcg cttttattgc ttgaattggt
    18601 acgaaaatat caatctggtt taaaacgatt ggtatttagt tcatcagctg cagtttatgg
    18661 cgatgaacca acgttgccaa aaaaagaaga atcggtcatc cgtcctttaa caccatatgc
    18721 catcgataag tttgcatcag aacaatatgt tttggattac tgtcatttat atgatgttcc
    18781 gactagtgct gtccgatttt ttaacgttta tggtccaaat caaaatccta actctccata
    18841 tagcggagtt atttcgattc tagtggaccg atacaaaaaa caattggcag gagaaaaaac
    18901 agaatttact ttatttggtg atggtagcca atctagagac ttcgtttata tagaagatgt
    18961 tatacaagca cttttacttg ttgctaagga agaaaaagct ttaggacagc aattcaatgt
    19021 cggtacagga aaaagcacta cgctacttga attgattcat tcaattgatc aaatcttagg
    19081 aacagagcta gcgcttaaat atgaagcgga gcgttcaggg gatattcgtg attctttagc
    19141 ggatatttca aagatccgtt ctttaggtta tcaacccaaa tttgatatat tgaacggaat
    19201 ggaacgttat ttaaagacag aaatcgattg aaacaaaaaa ccagcatcct gctggttttt
    19261 ttgtctcgac ttagcttttt aaattaacac cttccatcag ttcgtaaatc tgatttctta
    19321 aatcagtggc cggcaatttg atgtttttat cagattggat attactttcc atgacgattg
    19381 catcttttgt gtcttttttc atcgccgcta ctgcatggaa tcgattgata tttccctgag
    19441 caaggatata atcatttcca ctgtaaacac cgccgctgta aggatgttta tcaccattat
    19501 tttgccacaa tgagaaaagt agttcttttg gtataatatc gccagacaga acactggtaa
    19561 agaatttgtt caagtcataa acagacatac ttatatttcc tgtgcctaat tcatttcgga
    19621 tgtctgtttc aggctcgacc aatgcttttg aataatcgtc agttgcagac atttggtaag
    19681 atacagcatg ctgaggggaa ttttttacat tttcataaaa atctgtatgt tctagatgga
    19741 gtggccgaat gatttctttt tttaaaagag tttcataagg ctgattcatt agtttgatca
    19801 atacccctgt aagcaatgta taatttaatg gttcataatg atacgtatca taaccagtaa
    19861 atttcaaatg atgaagggca aactgaatga tctcactatc tgacataggt ttttttggag
    19921 aagcggttcg atgaagacca gagcgcatgt aaagcatatt tttgattgag atttgctgac
    19981 ttccaggaat ctgaggataa aattcagata gctttgtgtc aaaagttatc ttgccagatt
    20041 gaatttgttt taggataagt aaagcagtca tcgctttttg gatcgaaccg atttgatagt
    20101 aaagagaagg agaattcgat tcttttatgc gtagatttgc gtacccatat cctttttcta
    20161 atatcactcg attatttttg cgtacataaa tcgaaccgat ataatctgat ttttgcagta
    20221 attgatcaat tttgctggca agcatctgct cattagatgt taaattggaa gcaagttgtg
    20281 cgggttccgt tttataaatt tgtggttgag gttgttttgt taattgccat cctgtaatga
    20341 tgcctgttga aaagaagctg acagccagca acaaaactgc gagaaaagaa agagagatct
    20401 gctgtttttt ttgatgtttt ccttttgcca tattaaatcc tttctatcca tttttaacta
    20461 tttttcattg taatcctcaa aaagagcatt tgccacttac aattttgaca taatatttgg
    20521 taaaataatt cacggcagta gaaatttcat gtataataaa cagagttacg aagatgatag
    20581 taaggaggag ttgttttgtc aggaaacaaa caacacccaa gcaaagacaa aaaagaagcc
    20641 agtaaggcag ctacagcaga tataatcgac aagcggagaa atctgcgtaa aaaagaagat
    20701 aagatcgtga ggaagatcat tttagtgatc gctcttacat tattgattat tggcggattt
    20761 ctcgggttta ccgtatatcg ttatgtagat agcgggttga agcctttaga taaatcggac
    20821 gaccaattag tccaagtgga aatcccaaac ggctcttcta ataagcagat tggcgaaatt
    20881 ttggagaaag acaatattat taaaagtggg attgttttca attattatac aaaatttaaa
    20941 aacttaactg gctttcaagc tggttattat cagttagcgc ctaatatgac tcttgatgag
    21001 attggcaagc agttgcaaga aggcggcaca tctgagccaa caaaagttgc agatgggaaa
    21061 attgccattc cagaaggcta cgacatcgat cagattgctg agcgggtagc gaaggtaaca
    21121 ggtaaagaca agaaagaatt tttagattta gtaaatgatg agactttctt taacgagttg
    21181 tatcaaaaat tcccagaatt gttggaaagt gcctctaaag ctgagaacac aaaataccgt
    21241 ttggaaggct acttattccc agctacttat gattactaca cgagtacttc gcttaaggat
    21301 cttgtgattg agatggtgaa caagacaaat acggtcatgc aaaactatta ttcagctatc
    21361 aaacagaaaa atttgactgt acaagaagtt ttaactttgg cttctctagt tgaaaaagaa
    21421 ggcgtaaaag aaaatgatcg taaaaatatt gcacaagtct tctttaaccg aatcaaggca
    21481 aatatgccac ttcagtcaga tatctctgtt ttatatgcat taggagaaca taaagaattg
    21541 gtgacgtatg aagatacagc tgtagattca ccatacaatt tgtataccaa taccggctat
    21601 gggcctggtc cttttgacaa tccaagcgaa aaggcaatca aggcagtatt agaaccagca
    21661 gaaaatgact attattattt cgtagcagat acgagcactg gaaatgttta tttcgctaaa
    21721 acgtatgaag aacatatgga actagtgcaa aaatatgtaa ataattctta gaaaaacagg
    21781 ggctagtcat tgatcatttt tagaaaaaca tttacatttt aagaaaagcg tggtaatctt
    21841 ttttggacta tattcctcaa aagattacct tttgcggtat tttatcgaaa aaaggagacg
    21901 ttacacatgg tagagaaagt ttatccaatg actctagaag gtaaagaaaa attagaaaaa
    21961 gaattagaag aactaaaaac agttaaacgc ggagaaatca ttgaacgaat caaaatcgct
    22021 cgcggtttcg gagatctttc cgaaaactcc gaatacgaat cagctaaaga tgaacaagca
    22081 tttgttgaag gaagaatcac aacaattgaa aatatgatcc gttttgcaca aatcatcgat
    22141 aatgacggcg tagatgcaga tgaggtatcg atcggtaaaa cagttacgtt catcgagttg
    22201 cctgatggag atgaagaaga gtacacgatt gtcggaagtg ctgaagcaga cccgttttct
    22261 ggaaagattt ccaatgattc tcctatcgcg caagcattga ttggcaaacg taaaaatgat
    22321 gaagtaacca ttgctacacc aggtggcgat atgttagtta aaatcgtcaa agtagaacaa
    22381 gcgtagtctg actgaaagat ctagagagtt catcttactg atgacgcact ctagatcttt
    22441 tctctttgag tattgcaggt ttcactgaac gatcttacat ctaaagtcct atagccaaca
    22501 gtcaaaaaaa taggtatgat gaaatgagaa tgtgtcagga gggagaaaaa gcgatgaata
    22561 gttcttggcg tttttttgtg gtagtcgaag cactgctgct gttatttgct gtttggcaaa
    22621 tcgtaaacaa tactggactt ttgcttttag taatattcgg tattttcaat atatatctag
    22681 ctatgcgcaa atatcctcgt actaaatttc aaaattttca attgatctta gggagcttgg
    22741 tcattttttt tagcttagtc aacagtcctg ctttatggat gatggttgta ttggccgttt
    22801 tatttatcgg gctgaaaggt gtagagattt caggaataga tttgacaaaa aatgcttttt
    22861 ggcgaaaaaa acagatcatg atggtccaaa cagagcaatt aaaaacacat aataatgagc
    22921 gtaaaagaca gcaattattt ggtaaccaac gtattggaaa cgacgtctat gaatgggatg
    22981 atatcaacat tgccatcatt tctggtgata ctatcatcga tttgggtaat acacttttgc
    23041 caaaagatga taatattgtg attgtaagaa aagggatagg cagaacacgg atcttagtac
    23101 ccctgggagt ggctattcgt ttagagcatg cgacattagt aggaaatgtc ttgtttgaag
    23161 aagagcagtt ttccttaaaa aacgaacaga tcaaaatctg cagcagtgat tatgatgaaa
    23221 atcctcgcag attgaagatc attaccaata ccttggttgg agatgtcgag gtgatacgaa
    23281 tatgatggga agaatatcca aagcaatgct agctgtttat tcggggattg cggctttcct
    23341 tattattcta ttttcacttt tcacttattt ttatgccagt aaccaaagtc attggtgggg
    23401 agaattgctg cgtgcgcgtt tattatacgt tccgcttatc ttccatttga tggcaatttc
    23461 attaggtgta ggattgatcg tatttctatt gttatcactt attcaaaagg caaaatacgg
    23521 gaaaatcgaa gaaaagcttc gtgcactttc ttctggcaat tatgaatcca aacttttgca
    23581 tcagccgatc ccaagcgcgt cggatgatct gtacatcaaa gatattgata tggaaattgc
    23641 taaaataaaa gaaaaaatga tcgaagtatc cagtgaatta cagattatga ctagccgtcc
    23701 tcaatatgta gatggacaaa ccaaagaaga gattttagaa ttagaaaggc atcgattagc
    23761 tcgtgagctt catgattcag tttcacagca attatttgca gcgatgatga tgatgtcggc
    23821 attgacggag caagcagaaa aaagtgagac accagaaatg ttccgtaagc agttgaaaat
    23881 ggtggcagag atcatcaacg cttcccagtc tgagatgcgt gcgctgcttc ttcatctgcg
    23941 tccagtcaat ttagaagaaa aaagtttaaa acaaggcatt gagcagttat tgaaggaatt
    24001 gcagaataaa atccagattt cgctaaaatg ggatgtagaa gatgtaaaat taactagttc
    24061 cattgaggat cacttgttcc gaatcgttca agaattgtta tcaaacacat taagacatgc
    24121 taaggctaat gaattagaag tctatttgca taaaatagat aacaatcttt tattacgtat
    24181 cattgacgac gggacaggat ttaatatgaa tgaaaccaaa acgggaagtt atggattgaa
    24241 caatatcaaa gaacgagtag ctggtattgg tggaacagta aaaatcatta gttttaaagg
    24301 gcaaggtacg agcgtggaga tcaaggtccc tttgatgaag gaggcataga taaagatgat
    24361 aaaagtttta ttagtagatg accatgaaat ggtacgctta ggcgtctctt cttatttatc
    24421 tatccaagaa gacattgaag tgattggaga agctgaaaat ggacgtcaag gctatgaaaa
    24481 ggcgatggca cttcgaccag atgtcatttt aatggatcta gtcatggaag aaatggatgg
    24541 catcgaatca acaaaagcaa tcttaaaaga ttgtccaaaa gccaaaatca ttatcgtcac
    24601 aagttttatt gatgatgaaa aagtttatcc tgcaattgaa gcaggagcag caggatattt
    24661 gttgaagacg tctaccgcac atgaaatagc tgatgctata agggctacgc aacgcggcga
    24721 gcgagtattg gaacctgaag tgacaacaaa aatgatggaa aaaatgagtc ggcgaaatga
    24781 tccagtattg catgaagaat taacgaatcg agaaaatgaa attttgatgc tgatttctga
    24841 agggaaaagt aatcaggaaa tcgcggatga attgtttatc acactgaaaa cagttaaaac
    24901 gcatgtgtcg aatatattag ctaagttaga ggtagaagac cgaactcaag cagctatcta
    24961 tgcgtttaaa catggtttgg tgaaataaaa acgatgataa agaggataat caaaagctga
    25021 gaaaataact attatttttc ttggcttttt tacaataatg atgcttgctt ttgtttttgg
    25081 aaaggctttg ttatactgag aaaagaagat actaaaggag gaaaatggtg aaacaaaatt
    25141 ttgcaattgt aggccttgga cgatttggcg gaagcatctg ccgtacactg atcgaagctg
    25201 gacaagaagt actggctatt gacagtaatg aagatcgagt caatgaatat atgaatatag
    25261 ccactcatgc tgttgtggca aatgcacagg atgagatgac acttcgttca ttaggtataa
    25321 gaaatttcga tcatgtaatt gtcgctattg gagaagatat ccaagccagt atcttagtta
    25381 ctctgatggt aaaggaaatg ggcgtaccga atattttagc aaaagcccaa aatgagtacc
    25441 atgcccgtgt actagagaag atcggagcag atcgtgtcgt acaccctgag cgagacatgg
    25501 ggatacggat tgcacataac ttagtatcaa agaatattct agactactta gagctgtcag
    25561 ataaatattc tttagcagaa atccgagtct ccaatcctaa atttttcaac aaaacattag
    25621 ctgaattaaa tttcaggcaa cgttttgact taacagtagt cgccatacga agagtagatc
    25681 aaacggttat cgcttctcct gcagcagatg aatatgttcg cgaaaatgat aatctactgg
    25741 tgattggaga gacaggcgat gtggatattc tagacgataa gatgaatcaa tagaaagaaa
    25801 agagaaagta ggttgttggg atggaattaa cggtaacgcc aaaagcacaa aagtggtttg
    25861 actcagaaat tgatctgcca gaaacttacg gaatacgttt ttttggaaaa gtctacggca
    25921 aaacagaggt tcatgatggt ttttcgattg gaatgtcagt tgaacagcca gaacatcctg
    25981 taaagaaagt ggaaatggat gggacacttt tttttatcga ggaagcagat gaatggttct
    26041 ttaaaggata tgatttactt gttgattatg atcccaacct ggaggagcca acctatcact
    26101 ttaaaaaaca ataaaaaaag agagggtgac acaagttt
//
LOCUS       DACGLW010000035        26068 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.035, whole genome shotgun sequence.
ACCESSION   DACGLW010000035 DACGLW010000000
VERSION     DACGLW010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26068)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26068)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26068
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.035"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            complement(84..722)
                     /gene="plsY"
                     /locus_tag="IXT53_001461"
     CDS             complement(84..722)
                     /gene="plsY"
                     /locus_tag="IXT53_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HAQ4905293.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            849..1283
                     /locus_tag="IXT53_001462"
     CDS             849..1283
                     /locus_tag="IXT53_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HAQ4905294.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            1449..3494
                     /gene="parE"
                     /locus_tag="IXT53_001463"
     CDS             1449..3494
                     /gene="parE"
                     /locus_tag="IXT53_001463"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HAQ4905295.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            3506..5956
                     /gene="parC"
                     /locus_tag="IXT53_001464"
     CDS             3506..5956
                     /gene="parC"
                     /locus_tag="IXT53_001464"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HAQ4905296.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            6233..8464
                     /gene="pflB"
                     /locus_tag="IXT53_001465"
     CDS             6233..8464
                     /gene="pflB"
                     /locus_tag="IXT53_001465"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HAQ4905297.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            8708..9469
                     /gene="pflA"
                     /locus_tag="IXT53_001466"
     CDS             8708..9469
                     /gene="pflA"
                     /locus_tag="IXT53_001466"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HAQ4905298.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            9554..10480
                     /locus_tag="IXT53_001467"
     CDS             9554..10480
                     /locus_tag="IXT53_001467"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HAQ4905299.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            10662..11555
                     /locus_tag="IXT53_001468"
     CDS             10662..11555
                     /locus_tag="IXT53_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HAQ4905300.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            11689..11814
                     /locus_tag="IXT53_001469"
     CDS             11689..11814
                     /locus_tag="IXT53_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ4905301.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(11948..12580)
                     /locus_tag="IXT53_001470"
     CDS             complement(11948..12580)
                     /locus_tag="IXT53_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905302.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(12728..13270)
                     /locus_tag="IXT53_001471"
     CDS             complement(12728..13270)
                     /locus_tag="IXT53_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HAQ4905303.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            13405..13794
                     /locus_tag="IXT53_001472"
     CDS             13405..13794
                     /locus_tag="IXT53_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905304.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            13798..14562
                     /locus_tag="IXT53_001473"
     CDS             13798..14562
                     /locus_tag="IXT53_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ4905305.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            14691..15638
                     /locus_tag="IXT53_001474"
     CDS             14691..15638
                     /locus_tag="IXT53_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ4905306.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            15768..16508
                     /locus_tag="IXT53_001475"
     CDS             15768..16508
                     /locus_tag="IXT53_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905307.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(16799..17467)
                     /locus_tag="IXT53_001476"
     CDS             complement(16799..17467)
                     /locus_tag="IXT53_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ4905308.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            17601..17888
                     /locus_tag="IXT53_001477"
     CDS             17601..17888
                     /locus_tag="IXT53_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905309.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            18304..19842
                     /locus_tag="IXT53_001478"
     CDS             18304..19842
                     /locus_tag="IXT53_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HAQ4905310.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            19839..20804
                     /locus_tag="IXT53_001479"
     CDS             19839..20804
                     /locus_tag="IXT53_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4905311.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            20791..22113
                     /locus_tag="IXT53_001480"
     CDS             20791..22113
                     /locus_tag="IXT53_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ4905312.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      22176..22280
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            22354..23097
                     /locus_tag="IXT53_001481"
     CDS             22354..23097
                     /locus_tag="IXT53_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ4905313.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            23112..24104
                     /locus_tag="IXT53_001482"
     CDS             23112..24104
                     /locus_tag="IXT53_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ4905314.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            24116..24853
                     /locus_tag="IXT53_001483"
     CDS             24116..24853
                     /locus_tag="IXT53_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4905315.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            24843..25565
                     /locus_tag="IXT53_001484"
     CDS             24843..25565
                     /locus_tag="IXT53_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HAQ4905316.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(25649..>26068)
                     /locus_tag="IXT53_001485"
     CDS             complement(25649..>26068)
                     /locus_tag="IXT53_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HAQ4905317.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         8971 a         4430 c         5437 g         7230 t
ORIGIN      
        1 taaaaaagct atcctgtaag taataaggga gtgtgacatc gatttgtcac actcccgttt
       61 tatctctttg gaatgaaaaa gtatcaggat tttttcttgc tgctattcaa accaaacggg
      121 attcgacttt cagtatggtt tctgatacgc ttgatattgt ctttatgccg ataaaagata
      181 aaggaagaaa ctaaaaaggc aatgatcgtc agtaaccaat tgggcttagc caaaatcaca
      241 ggagctacat aaggaaggac gatcgtagat aaagtgatca atactgcact gatcatactg
      301 ctcaaactca ccatacttgt acaaaataaa ctaacgataa agatgataca agaataaaca
      361 aaaaatacag gattaaatcc taaaagcatt cctgcacttg tcgcaacagc ttttcctcct
      421 ttgaacccag caaaaatcgg aaatgtatgg ccaagaatgg cgcatacccc aaaccaaaga
      481 gggttaatcc cagaaaggtg gaagatcatc ggcaaacaag tagccaatgt accttttaaa
      541 atatccataa gcaggacaac aatcccggct gttttgccta atacacgaaa tgtattggtc
      601 gttccagtat taccgctccc gtgttgtcgg atatcttttt tgaaaaacaa tttgccaatc
      661 cagacaccgg aaggaataga acctaaaaga tatgctaaaa taagtaataa gatcaatttc
      721 attttttacc ctctttcaag ttgaaaacta tcctttattt tagcatgaat aggtaacagg
      781 aacaattgaa aaaatgaaag atacaagtat aatagatgga ataaaaagaa aagcaagggg
      841 tggcagaaat gtctgtaaaa aatccaacac aagaaacgat tttcaactat ttggaagaag
      901 caaaaaatat tgccgtcgta ggcttgagca ataaaccaga acgaacaagt tacatggttg
      961 cacaggtatt gcaagacaat ggatataaaa tcataccagt aaatccatta ttggctggtg
     1021 aagaaattct aggcgagaaa gtctatgcga atttgacaga aatcgatgat ccaatcgata
     1081 tcgtagatgt atttagacgg agtgaatttt tacctgaaat agcaggtgat tttttgaaaa
     1141 ctgatgctaa agtgttctgg gcacaattag ggattgaaaa caaagaagca gctgagatgc
     1201 taagaaaagc aggacgtaca gatatcgtaa tggatcgttg tattaagatt gaacttggta
     1261 agttgaaaga aaaccaaaaa taaaaatgac cacaaagaag agatgatcga tagaggggaa
     1321 tgtcgaaaaa gcggttagga gataattaaa attgatttct cgtctaccct cttttttttc
     1381 acgacctttt ctagtatgat aatcgtggtg cggttttgcc gcaaagtatt attgaaggag
     1441 cgtttgcctt ggcaaaaaaa gtaaacaacg aatacaatga ctcgtccatc caagtattgg
     1501 aaggattaga ggcagttaga aaaagaccag ggatgtacat tggatccact gacagccgag
     1561 gcttgcatca tttagtctat gaaatcgtcg ataatgcagt tgacgaagca ttatcaggtt
     1621 atggcagtga gattgatgtg accatccatg aagacaatag tataactgtt gcagatagcg
     1681 gacgtgggat gcctgtagga atgcatgctt ccggaatacc aacagttgaa gtgattttta
     1741 ctgtgctgca tgctggcgga aagtttggtc aaggagggta caaaacttct ggtggactgc
     1801 acggtgtagg agccagtgtg gtaaatgcct tatcgaaatg gctaattgta acgatcgtta
     1861 gagatggcgt agagtatcag cagaaattta aaaacggcgg taaacctgac ggtactttga
     1921 aaaagatagg aaaaacaaaa aaagcaaacg ggacgactgt ccatttttta ccagatgata
     1981 caatattcag tacaacgaaa ttttcatatg aaattttagc cgaacggtta agagaatctg
     2041 cctttcttct aaaaggcgtt aaaatctctc tttctgatct tcgtggagaa gaaccagtca
     2101 aggaaatttt tcattacgaa gaaggaatca aagaatttgt tgattatctg aatgaagaaa
     2161 aagatacatt gacaccagtt gtttattttt caggagaaaa agaaggtatc gaagtagagg
     2221 tcgcttatca atacaatgac ggttattcag aaaatgtttt atcttttgtc aataacgttc
     2281 ggacaaaaga tgggggaacc catgaagctg gtatgaaagc ggcgatgacg aaatcttata
     2341 atgaatatgc gagaaaagtt ggtttgctga aagaacggga caagaatctg gaaggtagtg
     2401 atttcagaga agggttagct gctgtacttt ctatccgtgt acctgaaaat cttctgcaat
     2461 ttgaagggca aacaaaagaa aaacttggta cgccagttgc tcgtaccgta gtagataacg
     2521 tcatcagtga acaaatgggc ttttatttgc aggaaaatag cgaaatgagc cagatgttgg
     2581 tccgtaaagc aatcaaagcc agagaagcga gagaagcagc acgtaaagcc agagaagaaa
     2641 gccgaaatgg gaagaagcgt aaaaaaggcg aatctcttct ttcaggaaaa ttgacacccg
     2701 cacaatcccg aaaccctaag aaaaatgaat tgtatttagt cgaaggggat tctgctggcg
     2761 gatctgctaa acaaggaaga gacagaaagt tccaagcaat tctaccttta cgaggaaaag
     2821 tcatcaatac cgaaaaagct aaaatgcaag atatcttgaa aaatgaagaa atcaatacga
     2881 tgatctatac gattggtgca ggagtcggtc ctgagttttc tatcgaagac tgtaattacg
     2941 acaaagtaat catcatgacc gatgcggata cagacggcgc ccatattcaa gtattactgc
     3001 ttactttctt ttatcgttat atgaaaccgc tgattgaagc tgggaaagta tatatcgcgt
     3061 taccgccttt atataaagta tccaaaggac aaggaaagaa gcaagtcatt gaatatgcgt
     3121 ggacagatga cgaattggca gcaatgatta aaaaagtcgg caaaggatat atgctgcagc
     3181 gatataaagg acttggagag atgaatgcgg aacaattgtg ggaaacaacg atggatccga
     3241 cttcccgtac gttgatccgc gtccgtatcg atgatgctgc gcaagctgaa cgtcgagtaa
     3301 ccacactgat gggtgacaaa gtagaacctc gaagaaaatg gatcgaaaat cacgtgcaat
     3361 ttacgctcga agaagatggc agtatcttag ataaaaaaga ggatactgaa atctctcctt
     3421 cagtttctaa tgatttatta gatgaagaac gagcagataa aaatgaaaac aatcagttat
     3481 ttgaagtaga ataggaggtc acctgatgga aaatcgacaa gaagtacaag aattaacttt
     3541 agaagaagtg atgggtgacc gcttcggacg ttattccaaa tatattatcc aagaacgtgc
     3601 actgcctgat atccgtgatg gattgaagcc agtgcaacgc cggatattat ttgccatgaa
     3661 caaagatggc aatacgtatg acaaaggatt ccgtaaatca gcaaaatctg tcggaaatat
     3721 catggggaat tatcatcctc atggtgacat cagtatttat gaagcgatgg tccgcatgag
     3781 ccaagactgg aagttgcggg aagtactgat cgaaatgcac gggaataacg ggagtatgga
     3841 cggtgatcca cctgctgcga tgcgttatac tgaagccaga ttatccaagt taagtggaga
     3901 gatgctagca gatatcgaga aagaaacagt cgatctcgtg tggaattttg atgatacaga
     3961 aaaagaacca acagtccttc cagctcgata cccaaacctt ctcgtcaatg gatcgacagg
     4021 tatatctgct ggatatgcaa cagaaatccc gactcataac ttagctgaag tgatcgatgg
     4081 cacaatctat atgatcgatc atccacaagc gagccttgaa aaattgatgg aatatattcc
     4141 tggacctgac tttccaacag gagggatctt gcaaggaaaa gatgaaatca aaaaagctta
     4201 tgaaacagga cgaggaaaag ttattcttcg ttcgaaaaca aagatagagg caatgaaggg
     4261 taataaacag caaatcgtga tcacagaaat cccatatgaa gtaaataaag cgaccttggt
     4321 gaaaaaaatg gatgagatcc gtttgaataa gaaaatcgat ggaatagctg aagtacgaga
     4381 tgaaagtgat agaacaggtc tgcaaattgt cgtcgaattg aaaaaagacg ccaatgctca
     4441 aggaattttg aattatttgt tcaaaaacac tgagctacag atcaattaca atttcaatat
     4501 ggtcgctatc gaccatatga ctccgcatca agttggactg aaagacattt taagaagtta
     4561 tatcgagcat cgaaagcaag tcataacaaa acgtagtcaa ttcgatttag ccaaagctca
     4621 aaaaagacag catatcgtag aaggattgat gaaagcttta tcgattctgg atgaagtaat
     4681 cgcaacgatt cgtgaaagta aagacaaaaa agacgccaaa aagaatttag tcgacgtctt
     4741 tcaatttaca gaagaacaag cagaagcaat tgtcacattg caattatacc gtttgacgaa
     4801 cacggatatc acagagcttc aaaaggaatc cgaaagcttg attgcgcaaa tcacagagtt
     4861 gaataaaatc ctttcaaatg acaaagagct tttctctgtt atgaaaaaag aattgcgaga
     4921 agtgaaaaaa aattattctt ccgctcgtct aacgacaatc gaagacgaaa tcgaagaaat
     4981 caaaattgat acgcaagtct tagtggcaca agaagacgta attgtcagtg tgacgcgaga
     5041 aggctatgtg aagcgaacaa gccttcgttc ttatagtgct tccaaaccag aagaaatcgg
     5101 gatgcgagaa ggcgactact tgctatactc aggtgaattg agtacattgg accatgttct
     5161 tctgatcaca aataaagcaa atgtgatcta ccgccctgtc catgaactac ccgatttgaa
     5221 atggaaagat gctggtgagc acatttctca aacaatctcc aatctgtctg tagacgaatc
     5281 gattttagct gttttcccat atcaaaaaat tcaagcagag aaaacatttg tattcatcag
     5341 caagaatgga ttgatcaagc aaacacgaat gacagagttc gaaccatggc gcacctataa
     5401 gagccgtccg ctcagctgta tgaagctaaa ggcggaagaa gatgaactga ttgctgtgta
     5461 tctgaaagaa gatcagccag gcttagatgt tttcttagta acgcatcaag gaatgggttt
     5521 gcgctatcct ttaacagatg ttcccgttgt tggagcaaag gcagctggag tgaagtctat
     5581 gaatttgaaa gatggagata ggattatcaa cgggctgctt gtgttagcag aaggtgatac
     5641 gccgatcgta atcgttactc agcgaggagc tgttaaacgc atgcttgctc aagaaatcag
     5701 ccaaacttca cgtgcgaagc ggggagtgac ggtcatgcgt gagctgaaaa aaaatccgca
     5761 ccgtattatc tatatgtcgg aaggaagatc aaaagaaata accttgatga atcaaaaagg
     5821 gcagcagtta acgattgacc caactgattt ccctataggt gatcgaacat cgaatggatc
     5881 atttgcgatg gatgagaaaa aaggtggaga agtcatcgaa gttatcgatg ctcctgagat
     5941 tttcatcgag tcgtaactgt tgtagtattt taatgaagag acgccatctg tattaataca
     6001 ggtggcgtct ctttttcatt catttttaat aaatccttat acgtttgcaa aaaaagatga
     6061 aagtgttcac aatgcttaaa tgttggtatg tcaacgttga tttaaatata tgtaaataaa
     6121 acataaaaat tgttaaataa aaaacaagaa aacccttgca aagatagggt gctgtgttat
     6181 actaatcacg taaaaactgt tacataaaac agaaatttag gaggtccata caatggagca
     6241 atggaatgga tttaaaggta ccaaatggaa agaaggcgta gacactagag actttattca
     6301 aaataactac actgaatatc gcggtgatga cagctttttg gaaggcccaa cagaagcaac
     6361 agaaaaatta tgggatgaat tgcaaaagtt aaatgatatc caattcgagc gtaacggtgt
     6421 ctatgatatg gacacagaaa tcgtatcaac tattacttca cacgatgcag gctatttgga
     6481 taaagatttg gaaaaaatcg tcggtctaca gacagacaaa cctttgaaac aagcattcat
     6541 gccgttcggc ggaatcaata tggctaacaa tgcgctaaaa agcaatggct atacaccaaa
     6601 cgatgaattg acacatattt tcactgactg gcgcaaaaca cataatcaag gtgtatttga
     6661 cgcttatact gctgaaatgc gtgctgctcg taaaaacaaa atcattacag ggcttccaga
     6721 cgcttacggt cgcggacgta tcatcggtga ctatcgtcgt gtggcattgt atggaatcga
     6781 ctatttgatg gaacaaaaga aaaaagatca tgatatgact ggttatcata caatgagtga
     6841 agaagtgatt cgtttacgtg aagaaatcac tgaacaatat cgtgcattag cgcaaatgaa
     6901 agaaatggct gcgaaatatg gctatgatat ttctaaacca gctgcaaatg caaaagaagc
     6961 tgtccaatgg ctatacttcg gatatttagc agcaatcaaa tcacaaaatg gtgctgcgat
     7021 gtcaatcgga cgtatctcag cattccttga tatctatatc caacgtgatt tggataatgg
     7081 agtaatcaca gaaaaagaag cacaagaatt gatcgaccac ttagtaatga aattacgtat
     7141 ggtcaaattc gcgcgtacac ctgaatataa ccaattattc tcaggattcc ctatctgggc
     7201 tactttatcc attgcaggta tgggattaga tggtcgttca ttagttacaa agaatgattt
     7261 ccgtctgctt catacattaa caaatatggg accttcacca gaaccaaact tgactgtttt
     7321 gtattctgaa caccttccag aaggattcag aacatatgct agcagaatcg ctatcgaaag
     7381 ttcttcgatt caattcgaaa atgataatca gcttcgtgaa caatggggtt ctgatgattg
     7441 tgcgattgct tgctgtgtat caggaacagt tgtcggaaaa gacatgcaat tctttggcgc
     7501 tcgtgctaac ttggctaaaa cactattata tgccatcaat ggcggagtcg atgaagtgac
     7561 tggcgcccaa gttggaccag aatatcgtcc aatcacatca gaaatactag actttgaaga
     7621 agtgaaacat aacttcatga atatgatgga ttggttagca gaactatatg tcaatacgtt
     7681 gaacatcatt cattatatgc atgataaata ttcttatgaa gcagctcagc tggctttgat
     7741 ggattctgac ttgcaacgca catttgctac aggtattgct gggatctctc atgcggcaga
     7801 cagcttgtca gcgatcaaat atgcaaaagt aaaagcaatc cgtaatgaaa aaggaatagc
     7861 agttgatttt gaagtggatg gcgacttccc taaatacgga aatgatgatg accgtgcaga
     7921 tgaattagtt tcatggatct tggaatactt catgggacaa atcaaacgtc ataaaactta
     7981 ccgcaactca aaaccaacaa cttcattatt gacgattact tcaaatgttg tttacggtaa
     8041 aaatacagga aatactcctg atggccgtga agctggaaaa ccacttgcgc caggagcaaa
     8101 tccaagttac aatgcggaac aaagtgggtt attagctagc ctgaactcaa cagctaaaat
     8161 gaattacggc tgtgcattgg atggaatttc aaatacccaa acaatcaatc cttcaggtct
     8221 tggaaaagaa caagatacaa gaatcgaaaa cttgcgtaat gtcttagatg gatacttctc
     8281 aaaaggagct tatcatttga atgtcaatgt attcagtaat gatttattgc gtgatgcaat
     8341 ggaaaatcca atgaaatatc cgaacttgac gatccgggta tctggttatg cagttaaatt
     8401 ccgtgacttg acaagagaac aacaattaga tgtcttgatg agaacttcac acgacagaat
     8461 gtaaaaagtt cgtgaaaaaa gtttttcgac ttagcgtcga aaggacgttg aatagccatc
     8521 gcatcaaaat cgaataatca gtatgcttat ttgaaagaaa cgatttacag gggcaggact
     8581 tgtatgttcc gtccctgtaa attttacaca taacgaagcg gcaattgcga aaaattttgt
     8641 tcagaaagct tcgcttcgac agctaacttg tacttaaaat gaaatcaaag aaaagatgtg
     8701 aacttaaatg gaagaaaaaa caatcggcta tgttcattct atcgaaactt tcggttcagt
     8761 agacggacct ggactgcgct ttgtcgtgtt tatgcaagga tgccgcatgc gttgccaatt
     8821 ttgtcacaat ccagatactt ggaatattgg cggagggaaa gaatacacag cagatgaact
     8881 tttagataaa gcagaacgct tcagacctta ctggggagat aaaggtggta ttactgttag
     8941 tggtggagaa cctctattgc agatcgattt tttgatcgaa ctatttaaaa aagcaaaaga
     9001 gcggaagatg catactacat tggatacatg cggaaaacct tttacgtatg aagaaccttt
     9061 cttttcacgt tttcaagaat tgatgaagta tacagattta ctgttatttg acatcaaaca
     9121 catcgataat gaagagcata aaaaactaac gcattgggac aatgaaaata ttttagaaat
     9181 ggctcaatat ttgtcaaaaa tcaacaaacc ggtttggatt cgccatgtac tagttcctga
     9241 gcgaagtgat tatgatgaat atttgatccg actagacaac tttattcaaa ccttgtcaaa
     9301 tgtagacaga gtagagattc ttccttatca tacgatggga aaatacaagt gggaaacact
     9361 tggtttgaaa tatccattag aagggatcga accacctaca aaagaacgtg tcgaaaatgc
     9421 taaacgattg cttcatacag aagagtatac aaaatattta acgcgatagg tcgcatcgga
     9481 aagaagataa gtattgcttc tttccgtttt tttgctatta ttaaatagga gaaatcacaa
     9541 aggagcgaat gacatgtcaa aaattttagt ttttggtcac caaaatccag atacagatgc
     9601 aatcggggca gccattgcgt tttcacactt gcaaaatgaa ttaggtaaag aaacagaagc
     9661 agttgctcta ggtacaccta atgaagaaac acaatacgca ttagaccact ttcagctatc
     9721 tgcaccacga gtagtagagt cagctaaagc agaaaatgca gatgtcatgc ttgtggatca
     9781 taatgaattc caacaaagta tttcagatat cagcgaagta aatattttag ctgtagtaga
     9841 ccaccatcgt attgcaaact ttgaaacagc taatccattg tactatcgtg cagaaccagt
     9901 tggatgtaca agtacgatta ttttaaaact atataaagaa aaccaagtaa caattcctaa
     9961 agaaatcgca ggaatcatgc tctcagctat catttcagat acattgttgt tcaaatcacc
    10021 aacatgtacg cctgaagatg aagcagctgc aaaagaactt gcagcaatcg ctgaagtcga
    10081 tctaaacact tatggacttg aaatgttgaa agcaggaaca aatctaagtg ataaatcagc
    10141 tgcggttctt cttgatttgg atgcaaaaag tttcccaatg ggcagttcca acgttcgtat
    10201 cgctcaagtc aatacagtgg accttaatga agtgatggat cgacaagcag agctagaagc
    10261 agcaatggaa gatgaaatga aagcaaatgg ctacgatctg ttcgtattag ttgtcacaaa
    10321 catcttagac agtgattcag aattacttgt tgctggtaaa ggaaaagaaa aagtagaaga
    10381 agctttccaa acaactttga ccaacaaccg tgctttgctt aaaggtgtcg tttcacgtaa
    10441 aaaacaagtc gtacctcaat tgacggctgt atttagctaa tcaaatggta gtatgaagca
    10501 ttcaaaatga atggataagc gattgatata aaaagaaaca tagaacatac taaaggccga
    10561 agaaatggag gagaataaca gtcaattatg actcttgttc ttcctccttt ttacgtaaaa
    10621 gccttttaag taaaataaaa cggatagaaa agagaacata tatgaactat tattcaaaaa
    10681 ataaggaaaa cttttatcaa aaactgaccg atgatccgct tttttcttca ttgaccgatt
    10741 atctatatga acatcgagaa caagaaacga ttctaagaga attgaaaaaa gaattttcgc
    10801 aaaataaatt ttcccatttt cttgatttat tgatcgatgc aggattaatc aagagggaag
    10861 aacgaagata tcatttgaac tttccaatat ttgatcctaa agattacttg caacaagcca
    10921 cttcggctgc tgaaaccata gctgaacaat tgaagagact ctctgttgat gaacaaaaat
    10981 tagcgatggg agaagttatt tgggcgtatt gtttcgaaga tgaacggaaa gaagcgtatt
    11041 tttatggagt aagaaacagc cgagagacag aattgctgag gacaactgca ggaaatgaga
    11101 agtaccgttt tatcacactt tcctctatag aacacttccc gcttactttg gctaattact
    11161 tttttgtaca gaagaatcag cttcctgtaa cgaaggcatt caaagaatta gccgaactta
    11221 ttggagacgt aaatgaatcg tacttttttg atcaaataga agttatcgtt gacaggatac
    11281 gtaagaataa atataaaaat cgccggccta gtattttcca tcagtctcta cttgtgacga
    11341 atactataaa ggaagaagaa tcattcacat tagaattacc tattgtcgaa aaaaacaatt
    11401 ttgagattga attaccaaca ttggatcctt cattgacaat ggaggaaatg gcctttctta
    11461 aacgacaaat cttttctgag ctgagcaaga aatttatacc gcatgcattt tcttatatca
    11521 aagaatatgg aacagtttta gtgagtaaaa cctaagagag cagtaagcaa aaattatact
    11581 gactcttgca ggaggagcct ttctcttcct ttatggttaa aagaattatt tgagtatttt
    11641 gcctatctac cacctattag attcactaca tggtctggaa acaaaggaat gcaaaaaaat
    11701 ggtgagaaat gtggaatgac aaaggaagta gtgataagaa aagttagatt tttaaataat
    11761 caatattatg attcagtcaa gtatggcatt ttatgggaag aactagctga ttagattagg
    11821 aagtccattt gataatccag aatcactaag aaacgaatgg cgagtcatta ataatttatc
    11881 caatcttggg ttattctttt agattgaagt gtttaaaact aaaaaagcat ttatgaagtc
    11941 gtaatagtca ggcttcaaaa tgctttttat tttgtttgtt ttttctttgc cacataaata
    12001 cacctagact gacgatgaag agcatatcca acagatttcc tgcgacttgg atcgtcgaag
    12061 ggttggtcag tggcattaaa gcaaggacaa aaaaggaaaa caagctgtaa ataaaacagg
    12121 tgacaggaac taatcgaaag aagagcggac atttcaattg gctaaaaatg agtgaaaaaa
    12181 taaaataagt aaaaatggat aaaatcacga ctaaaccatt catgataaag aagaaaagcg
    12241 tattgtgatt aaacgaataa aaaagaattg ctaaaaaaga aaacgtttta aatgcaatca
    12301 atacgcttga agcttcgttt aacgtaccag gatgtcgatg aaaaaaatag gcaccgaaat
    12361 aaacagaaaa cagtaagccg caaagagaag acaaaatcaa aaaaaacgga ttttcttcgg
    12421 cttctgtgat atcagaatga tccaaaagca accagcctaa tgcactagaa ggcgcatgtg
    12481 aggtcttaag gtatgaataa catagaaaaa ataagatcgt caaagttgca aaaaaagagc
    12541 ctaaatgtaa aaaattagtg gttctacgtt gaatttccat aatatctcca aattaaattg
    12601 ataagtatat aaagtataat aacaaattga tgagtatatg tcgatgtttt ttaatcaaaa
    12661 taataaataa aaactgtcta acatttaaaa tttaatttag atattagaca gtttttaagt
    12721 ttgtgagtta ttttccttgt cgttttctca cttttttagt ccagaaacca cctgaaatat
    12781 attttggctc ataagaaatg ataaaagcac gctcttcgat ttctttgatc aggcgataca
    12841 gttcacgttc gctttttcta ggagaaagta tttcgagaac tagccgctca ccttccagcc
    12901 ccattgcaga agtctgtgtc acaccataac cgtgttctct cagtataccc ggcaaacttt
    12961 tatcttctgc gctatttgct ggcaaaatcg ttgtgaccat gatgtatcct aatgccaatt
    13021 tgtcttcaat caaaattccg acactgatac ctactgcgta acctaacgca tagacaaata
    13081 aattcagtgg attatctaaa cgatctaaaa caagggataa gccgacgata taaatcgtga
    13141 tctcgaccat actgacaagt ggagcaatca gtcgatagcc tttcattgtc aacatgaaac
    13201 gtaacgtgtt cagagtgata taagcaaaat taatgaggaa gatcatcccc aacatttttg
    13261 gatcaatcat ttgtaaaact cctttcattt atataacaac aatatcaaaa aaaatcgtaa
    13321 gtgaagaaaa attgaaactt tatacataca aaaaaatgat aaaatgatta taatagaatt
    13381 ataaagatag gttaggtgat taaaatgaaa acggtcaatc gtgtgttttg tgccagccac
    13441 gtgctaagtc tccttctcag tatatactta ggtgtctatt cgattcaaca gccaattgag
    13501 gtagagaaca ttgtttttca gatgtcgctg acagatggtt taattttaag cgtttttttc
    13561 tttttgatac tgtttattgg taatattttc ggtagcgttg ccagtttttt gaatttttct
    13621 atttatccac tcctttcgct agcattagga gtggtgggac tttgttctct gtgcttattt
    13681 cccgagccat tcagtcctgc atttatatta tttggtacac tgaacctgtt ccagacaact
    13741 gttggagcat ggttgctatg gcgatcagga aatttaatga aaataggtga gtaacaattg
    13801 aatccaatta tattgaaacg ttctgtccat catattccag tgttagaagt tgttctgaaa
    13861 gaattaagag aggaacgtat tcccgttgtc atctattatc acggatggca gacaagtaag
    13921 gagttagttt tgacgcaagg aagaaaaatt gcgcagcagg gcattcgtgt gatcctgcca
    13981 gatgcgatga atcatgggga gcgcaaacag ccggtttctt ctattccatc ctatacattt
    14041 tgggatagta tctacggaaa tctgtttgaa tttgacacac ttatcaagca tttagaaaaa
    14101 agaggtcttc tacatgatca gcttgcagtt ggcggggttt cgatgggggg aatgacaaca
    14161 tgcgccttgt tggctaaaca tcctcatata gtagcgggcg tgtgtttgat gggttcccct
    14221 gcacctttgg aatatggaaa agaaatcgta aggcgcgcat cggaatataa ttatgtcgta
    14281 ccaaaagact attttgattt gatttcatgg gttaagagct atgatttgtc attgcagccg
    14341 gaaaagttag ctggacgtcc tctgttcttt tggcatggac aagctgacga aaaaatccct
    14401 tatatacaaa cggaacgatt tgtagaaaaa aatcggcata agtcttacgg aaaagaaatt
    14461 gtttttcgtt cctcaaaaac acagcgacat atggtacaaa taccactaat ggaagaagct
    14521 tccacttttt tagcaaaaaa tttgataaaa aagtataaat aattgaaacg gaaaatcact
    14581 catgacgata gaggattttc cgttttttat tttgggctat aataaactct gttaggaaaa
    14641 tttggtataa ttgatagtaa gaatgacaac atgttagaaa ggaagagagc atgcccactt
    14701 tagcagatgt tgcaaaacgt gccaatgttt caaaaatgac ggtctctcga gtcatcaatc
    14761 atccggaaca ggtgactgac gaattgaaag agttagtttt ctcagcaatg gctgaactgg
    14821 attaccgtcc aaatattgct gcaaaagcac ttgtcacgaa tcggtcgcag atcatcaaat
    14881 tatttatact ggaagaaatc gacacaactg agccctatta catgaatttg ctgatgggaa
    14941 tcgctaaacg gatcggtaaa gcgcattatt ctctccaatt agttacaaat gatgccttcg
    15001 acgtcggttc ttgtgatgga tacatcatca caggtgtcag aaaaaaagac ttcgattgga
    15061 tcgaccgttt ggaaaaacca gttgtcttgt ttggggaaaa ttctgaagga tatgattatg
    15121 tagacagcga taatgaatat ggtacagcaa aagcaacgca atatgcctta gaaagagatt
    15181 atcaaacgat tatttatatc ggtatcgaag tggacgagcc ttttgaaatt tcaagggaaa
    15241 ctggttattt atctgtgatg aaggaagttg atcttcctag taaaatcttg cggtttccaa
    15301 atcattcgac agaagtagaa agatatatga cagaaaactg ggcagatttt acagataaaa
    15361 cttgttttgt atgtagttca gatcggctag cactaggagc agtaagaggg attataaatg
    15421 ctggaggaca cttgcccgaa gaatttggtg tgattggatt tgatggggta tttttagacc
    15481 aaatcagttc acctaaattg acaacgatca agcaagatat tatacgtatg ggtgaagtat
    15541 gtggagaaat gttgttaaaa aaaatagaag aaaaaggggc gagtcaaggt tatcgtcgtt
    15601 tttttcctga actagttatc agagagacaa cgaattaagt cttattacta tatatatatt
    15661 tatgaaattc atcgctattt tcttaaaata atcagcgcat tgctactgaa aacaatgaat
    15721 ctgtgctatt attgagaaaa gttccatttt tgagaaggat ggtttgaatg aaagaaatca
    15781 gacgaaaaca tttccgtttt cctgcgtacg atgatgagtt aggcgtaaaa ttaaccaacg
    15841 acaagaagcg agtattattt gacggacaag aagatctttt gactgaacag aatcagtccc
    15901 cgtttgaaca gatgaaagaa tttcatttca cgaacacgaa caccgagaaa atccgtcgca
    15961 agcgtcgtgc aacaaatgga ttgaagtcag ctccgcgtaa ggaaaatttg gaaaagcata
    16021 aaggaaaatt accggattat accggccatt accaatcaga aatcacatcg acaggacgaa
    16081 aaaaactttt tggtgattcc tctcgttctg tcagacaaag tgaaacaagg acagagtcca
    16141 agcatactac acgaaacgca tctgttgaca cgaaggtgcg ggagcgttct tattttgttc
    16201 ctaaatatgt acccgcatcg attatcccag accctagaga aagcaaaatt tctgaggatc
    16261 atttaatcga atcgatgaaa aaaacggatg actcttatct gttgttcgat acagaaccga
    16321 cgccgtatca agaaaaaaga aatggtgagc cgtctgtacg aacattcaat cgaacaaaaa
    16381 cgttagagga aacaaagcag tcagaagaaa aaaaatcgaa aaaaagccat ggtatcttag
    16441 aacgctcatt acaaggattg atcgatgatc aaggaaactc tttgggagaa aatagctatt
    16501 ttaaataagt gatatacaaa tttatattcg gctacagttg gaaaggaaac gctgcggcgt
    16561 ttccttattt tatactctat ccgaaagttt atatcgtatc ctcaaatttt tttgagttta
    16621 agcgaatttt ttctcccaat aagtaagaaa ataatggcaa aataaaaaaa caaactattt
    16681 tcgcagatcg tttgcaaaaa tagtttattt ttttataaag aagattttac tgaataatat
    16741 cattttagtt tacataatga tatttctgta aattaaataa agtagtgaaa aataagaatc
    16801 agtgctctaa ctgttcaaga ggatcaagtg gttcaaatgt ttctgtagtc tgttttgctt
    16861 ccaattgggg caaaacagat ccaatttcca gatacttcac tttattcaat gtataagata
    16921 gaatagaaca gctaagtaat gtatacagaa aatgtgtgag atccaaataa agcaaggaga
    16981 caactaaaat accaaaagta atcaagaaaa gacttttgcc aatcttaata tgaaaatatt
    17041 tttcaatggc ttgataaatg atatctgttc cgcctgtgct tccgccgaat cgaaagacga
    17101 gaccgcaacc aatgccagaa agaatacctg tcatcaaact attgatcagt agactatcta
    17161 cagcaaaatg tatatgaagg ctctcccata aatgtaaaaa gaaaactagc gcaaatgttc
    17221 cgacgatcgt ccggagaaaa gcgtgttttc caaaaagaaa caatgagata agcaataaag
    17281 gtaaattaaa aagcagtgaa gtgacagaag gagcgaatcc aaaaagatta tgcaaaagca
    17341 aagcaatacc agtaacccca ccatctgcca gcttagatgg aatattgatt cctgtcacag
    17401 ctaatccgta gattcctgcg cctaaaatga ttccgcacaa ttctgtaaat aaatttaatt
    17461 ttttcatttc tagcctcttt tcttttgaaa attttcagca acttctttat tatactgact
    17521 tatactctta cttcaagagg aaaatgaaaa aaaattattt ttggctgttt ctttttattt
    17581 atcaaataag gagtggaaaa atgcgagcag cagacttatt gacaattctt acaaaatcac
    17641 ccaaaaaaac agagtatcaa ggagtttttt tggctcaaga acaaaaactg gtgcctgtga
    17701 caatggtcca gataaataaa gcaaatcaat ttatcttttt atttgaacac ggcaaagcac
    17761 cacttgctat gaaagacatc tttctttttt tgatgaagaa ccgtgagaaa gctgtttatt
    17821 atcagtcaaa agaagaactc attcctcttt atggagtgaa agagatagaa aataaagtgg
    17881 tgatataagt aaaaataaga aaacaagacg tttatgatgt caaaacaacc attttttaca
    17941 tttttggcaa aaaagtgtca tgatacagta taatattcgt tgaacaggcc aataaaggtg
    18001 ttctccccca cgagatttct actttattgg ctgtttcttt ttattaaaat aaaagttgtg
    18061 tgtgtaaaaa agatatagct gattccaagt gacaatttcg tatagatttt tatggtattc
    18121 gtagtgaaaa aacgagaacc aaaaagttga tccgactttg ttgcttggat tctttttttg
    18181 tcagcatatt tagaggtatt tttttctttt ttttgttcgt gctgtaaggt taaggcaaga
    18241 caagttagtt gggaaaaagg aaagaaatgt ttatacttaa agaataacag aaaggaaggt
    18301 cgcatgaata attttataca aacattaagt gaaagaaaag gcgagttgct ggaagctgca
    18361 tttcaacatc tgtccatttc tctcatttct ttgctgattg cagcactgat tgctattcct
    18421 ttagcgattt gggcagccaa tcataaaaaa atggcagaag tcttattgca gatcacgggt
    18481 gtgctccaaa cgattccttc acttgcctta ttaggtttac ttatcccatt tgtagggatc
    18541 ggaactgttc cagcattgat tgcacttgtt atttacgctt tgcttccgat tttccaaaac
    18601 acgtatattg gtctagctga aattgacccg tctattgaag aagcagcggt agcatttggt
    18661 atgtccagaa tgagacgctt gctgaaagta gagcttccca tcgcattacc tgtcattatc
    18721 tcaggtatcc gaactgcctt agttttaatc atcggtacgg ctactttggc agcattgatc
    18781 ggagcaggtg gattagggac atttattttg ttaggtattg accgaaatac acctgttttg
    18841 acattgattg gtgcgatcag ttctgccttg cttgcaatta tattcagcgg actgattcgc
    18901 tggttgcagc ataagaaacc tcgatattcc atcattactt tagcagcagt ggtgtgttta
    18961 tttggcggcg tttctttata tcagtctgcc ttgtttaaaa atgaaaccat cacgattgct
    19021 ggaaaattag gagcggaacc tgaaatcctg atccaaatgt ataaagaatt gattgaagac
    19081 aacacaaaaa cagaagttga attaaaaccg aattttggca agacgagttt tttgttcaat
    19141 gcactggatc aaggtcaaat cgatatctat cctgagttca caggtactgt cctagaaagc
    19201 ttggtgaagg tacctgaagc aatgaaaagt gctgatttga caaaagaaaa aacatatgac
    19261 gaagcagaag aattattgaa taagcaattc gatatgacat tattaaaacc aatggcttac
    19321 caaaatacgt atgccttggc agtcaagaga tcttttgcga acgaacatca acttaaaaag
    19381 atttccgatt tggcagctat ctctgatcag atcaaagcgg gattcacact ggaatttatt
    19441 gatcgttcag atggatataa aggaatccaa gaagcatatg gcttgaattt ttcaagtgta
    19501 caaagcatgg agccaagttt gcgttaccaa gcaatcaata atggagatgt caatattgtg
    19561 gatgcctatt ccacagatag ccagctcaag gagtacgatc tagtgactct agaagatgat
    19621 cgagggttat tcccagctta tcaaggcgca ccgctgatga aaaaagattt tgctaaagaa
    19681 catcctgaaa tcgttaaagc actgaataaa ttaagcggaa aaataacaga ggatcaaatg
    19741 attgatatga attaccaagt caatgtcgaa aagaaaaagc cgacagaagt agcacgaatc
    19801 ttcctgaaaa atgaaggatt agtaaaggag gaagatcagt gactaaatta atcgagttca
    19861 aacatgtgca aaaaaagtat gatggaaagt atgtcatcga tgacctaaat ctggcaatca
    19921 aaaaaggaga aatctttgtt ttggttggtc cttccggtag tggaaaaaca actactttga
    19981 aaatgatcaa tggattatct aaaccatcag caggagacat ctacttcaaa ggaaaatcgt
    20041 taaatgaata taatctgcag aaaatgcggt ggaatatggg atatgtgctg cagcaaatcg
    20101 ctttgttccc gaccatgaca gtcaagcaaa atattgaagt catccctgaa atgttaggtt
    20161 gggaaaagca gaaaagagca gaccgtgtag atgaacttct tcaaaaagtt ggcttatctc
    20221 ctgatattta tcgagatcgt atgcctagag agttgtctgg aggagaacag caacggatcg
    20281 ggatcatccg agctattgct gcaagcccag atgtgatttt gatggacgag ccatttagcg
    20341 cattagatcc gatttcaaga aactcattac aagaattggt tttgtcctta cacgaagaat
    20401 tgggcacgac catcgttttc gttacgcaca atatggaaga agcaatcaag ctaggtgatc
    20461 gtattgcatt tatgaaggat ggagaaatta tccaatgtga cacaccagag cagttattga
    20521 tgaatcctaa aaacgactac gttcggcatt tctttgatga accgaaacag acgaaagagt
    20581 ggcgagtaga agatttagtc gtcaatggat atttcttaaa tgaaatacca gaaaatgctc
    20641 gtcaacaagt ttgctttgat acgcctatga aagaagtata ttcgcttctt tcggtgtatc
    20701 caagcattgt gatagtagaa aagcaaagaa gtattgggtc gctgacaagt aaagaaatct
    20761 ttgctttttt aagtcgagag gaggaaggaa atgaaaacat atgatgcaat catcatcggt
    20821 tcaggtgtga gcggcttaag tgctgcttat ggattgaaag aagcaggtaa aacagtacta
    20881 gtggtcgaag aagatctatg gggcgggact tgtcctaacc gtggatgtga tcctaaaaaa
    20941 gtcttgttaa gtgcagtaga ggcacgtaat cgggtaaaac aattgagtgg aaaaggtttt
    21001 aacgagatcc caactgccaa ctgggaagaa cttcaaaaat tcaaacgaac atttacggat
    21061 ccagtcccag aaagccgaaa aaaacaatta gcagaagctg aaatcgatca tctttcagga
    21121 accgctcgtt ttttggatga ttcatctatt gaagtcaacg aagaagtctt ccatgcggat
    21181 tacttagttt tggcaaccgg gcaaagacct acgatcttac cagtggaggg aaaggaatac
    21241 ttgaaaacta gtgcagactt tttatcattg cctgtactgc ctaaagaaat catttttatc
    21301 ggtggtggtt atattgcttt tgagctagct acgattgcca atgctgcagg gagcaaagta
    21361 accattgtcc atcataatca acggccatta aaagaatttg aggcgtcttt ggtagaggaa
    21421 gccgtccatc aaatggaagc atctggcatt cagtttgctt ttggtgttga aacccaaaag
    21481 atcatttcag aaggtactcg ctatcgttta gtcggaaaag agacggaatt agtagcagat
    21541 atgatttttt gtgcgactgg gagacagccg aatacagaat ctttagcact tgagcaagcg
    21601 aatatcgtct ttgataaaca cggaattgca gtcaatgatt atctgcaaac gagcaatcca
    21661 aaaatttttg cgtgtggaga tattgtttca aggaaaaccc caaaattgac accagtcgcc
    21721 acttttgaag gaaattatgt agctaagcgg ataacggatg caacaagcga acctatcaaa
    21781 tatccgatta tacctacgat cgtctacgcc agtcctaaac ttgctgaagt aggagttaca
    21841 aaaagtcacg cctcttcgtc agatcaagtg gtagagatgg atcttaccag ttggttcacc
    21901 tatcatcgtg tcaatgaacc agttgctaag gctgaattga cctttgacca gcaaaattat
    21961 ttgattggtg ctgcagtgat tagcgagcaa gcagacgaat tgatcgatga tttgacactc
    22021 gtcatcaatc aaaaattgac taaaaaagag ttggatagct atatcatggg gtatccgaca
    22081 ttggcaagtg atctctccta tttattgaaa taatcagcat gataactaaa gcgcttgttg
    22141 ctttttcctt cttgtgctac actttaaaaa atgaatacct gtttctgggg atgtcgtaga
    22201 cgacatgaga gtaagttgtg aacttagacc cattgaacct gtcagttaat actggtgaag
    22261 ggagaaacat agcagacgct tgaagcgggt tactatgtcg tgcccatttc aagcgttttt
    22321 tgttattcat attagaagaa aggaggtaac aaaatgaaga aaaatcttta tccagcagtc
    22381 agtatggcat tcattttaat tatttggcag attgttgtca tagtttggaa aatacctgcc
    22441 tttattttac ccggacctgc tgctgtattt tctgcattag cggcagatgc acaagtatta
    22501 ctttcccatt ctgtcacgac attaaatgaa gcagtgcttg gtttggtaat agctagcgtt
    22561 ctttctttta ttactgcctt ggcaatggat tattggaagt ttttagaaat gtctttttat
    22621 ccgttactag tgatttcaca aatgttgcca atcatggtgt tagggccatt acttacccta
    22681 tggtttggtt tcggtatgct tccaaaagtg atccttgttg tcttgataag ctattttccg
    22741 atcgtagttt ctttttcgga tacattgagg aaagtatcaa aagagcaaat catctttttg
    22801 aaaacgatgg gggcagatac agggaagatt tatcgaatat ataaaatacc agcgggttta
    22861 tccggcttct tttccggttt aaaagtggct gctacttatt gtgtcagcgg agcagtcgtt
    22921 ggagaatggc taagcgctca agcaggctta gggtattaca tgatacgcgt aaaaaacagc
    22981 taccagatcg ataaagtttt tgctgcgatc atttgtgtca ttctattgag tttattgctg
    23041 aatggcgctt gttcattatt gaaaaaaggg tattatcgta tgctttatat tttttaagag
    23101 aaggggatca aatgaaaaaa ttatggcttt tattaccgct actgttgctt ttatccgctt
    23161 gtggcactgc aaaagaaaca agtagcaaac aagaaataaa ggatttgaaa aaagtgacac
    23221 ttgttttgga ttatgtaccg aatactaatc atactggcat ctacctagca aaagaaaaag
    23281 gctattataa agaggctggt ttaaatgtcc aaatcatcga accaggagat aattcgacaa
    23341 gtattggtct agtaggtgcg gacaaagcac aatttggtgt aagttatcaa gaagatgtca
    23401 cttatgctca cgcagatgga cagaatatac cagtcaaagc aattgctaca gtgatcaaac
    23461 ataatacttc tggattcgcc accctatcag acagtaacat ccattcacca aaagattttg
    23521 aaggaaaaac atatgcaggt tggcaaagtc caagtgaaga agctgtctta aaagctgtga
    23581 tggaaaaaga cggcggggac ttttcaaaac tgacaatggt aggaagcaat ggagaagggc
    23641 cagaaagctt aggaaaatcc tcagatatcc aatggtactt tgaaggttgg gatatgatca
    23701 aagcaaaaga agcgggtatt gaactaaact atatcccatt gaaagaatta gatgaacggt
    23761 tagattacta tactccggta attattacga atgatcaact gatcaaaagt gatccagaat
    23821 tggtacagtc ttttatggat gccacgaaaa aaggctatca ggaagctatt aaggatccaa
    23881 atgacagtgc taaactgttg caaaaatatg caaaagaaaa tgatcggacg tttttagaag
    23941 aatcacaagc attcttgtca aaaaattata cggatgatcc tgagaattgg ggattgatgg
    24001 aagaaaaagt ttggcgcaat tatacagctt ttatgcagga aaacggattg atcaagcaag
    24061 atgtaccttc gcaagaacta ttcactaatc agtttataaa ataggagtga tagatatgtc
    24121 aatccaaatc agccacttga atgtagtcat tgaaaacctg aaaatattag aagacatcca
    24181 tttcactatc cctaaagata cctttacatc gattgtcgcg ccaagcggag ccggtaagtc
    24241 aacattgctg aaaactttaa caggagtcca gcctatgact tctggaacga tcaaagtaga
    24301 cgatcaaaaa gtaactggtc tccatacagc atttagttat atgccgcaag aagatatgtt
    24361 acttccttgg ctgagcgtct atcaaaacgt tacgttatat caaaaaatca atcatctaac
    24421 aatcaatgaa gaacaagtaa gggattatct ggatatcttt gggttaaacg gctatgaaca
    24481 ttttttgccg gaacaattat ctggcgggat gaagcagcga acggccttgt tacgaacaat
    24541 catgaatcct tcttcttact tgcttcttga tgaaccattt ggagcgttag atgctatgac
    24601 cagagggcag atgcaagact ggatgttgaa gctgcctaaa aaagcaaagc ggactacttt
    24661 acttgtgacg catgatatcg aagaagcaat ttatttatca gacaggattc ttgtgctttc
    24721 cgcccgtccg gcacatgtga ttgctgagat tcaagtgcct gaaaaacgac ggtcacgtga
    24781 atggctactg cagcaatcag agttgaagca aacgatttat cagttattgg caggtgaatt
    24841 agatgttaag tgatgctcat tgtcatttag atgggagtca gagtttagca ctccttcagc
    24901 aggaacacac gattttgacg atcatcaatt gtgattcgcc agaagaatgg aaggaaaaca
    24961 gacagcttgc agcaagtaag actcaagcct taagttacgg tatccatcct tggaaagcag
    25021 atagctatac gtttgaacaa gttgagcctt ttttgaaaaa agcaaggatc atcggtgaaa
    25081 tagggttaga taatatttgg acgaatgtac cgatgactac acaaaaaaaa gtatttgagc
    25141 gacagcttgc ttttgcagcc ataaatgaga aacctgttgt actgcacaca aaaggatgcg
    25201 aaaaagagat cttaaattat atccaagctt accctaatca ctatttgatc cattggtatt
    25261 cttctttgga ctatcaacaa gactatattg atttaggctg ttatttcagc attggtcttg
    25321 atttgaaaaa gaatccagca gtctggcaac tagctaaagc agtcccaatc gaccacttac
    25381 ttattgaaac ggatggacta catgcagcag agtgggcttt aggggggatt ttttcagaaa
    25441 aagattatgt ctcactgctt gaagaacagt taaagacaat cgctgagatc aaagagctat
    25501 cagttgaaga agttaaaaaa aatacttatc aaaacttgga acgtttcatt agattaggta
    25561 aataaaaaag agatgtgaca caactagtgt cgcacctctt tttggctttc tttgtataaa
    25621 agtaaactgt ttgattaata aatgaatctt agtaccaacc gtttgcttcc cagaatgctt
    25681 tcgccgcttc ccaagaacca taacgtgaag taacatattg ttctgctact ctttcttggt
    25741 ttgcagcaga gtagtcacca tttaagtaag aagaatctaa ttggtaacgt ccgatgtaac
    25801 gtccgttcgt tgctgtgtaa gaaccgcttg attctttttg tgcgatccat tcttttgcag
    25861 agcttgtgct tgctggagta gcagcagttt ctgttacagc aggagcaggt tgtgtttcag
    25921 caacaggcgc tgcttgtttt tccgtttgaa ccgtttcttg tacaacaggt tgttcagctt
    25981 ggactggtgc ttcttgaacg gctgcttgag gttcagcagc tgcttgtgca gagccttctg
    26041 ttgggattgt taattgttgt cctgagta
//
LOCUS       DACGLW010000036        26051 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.036, whole genome shotgun sequence.
ACCESSION   DACGLW010000036 DACGLW010000000
VERSION     DACGLW010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26051
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.036"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            44..379
                     /locus_tag="IXT53_001486"
     CDS             44..379
                     /locus_tag="IXT53_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905318.1"
                     /translation="MKQYLNEWKAIEGSLVTQRIKELPDCLEKDHLFQIREMLKKEQF
                     DPDQFLVVEYPTIGVYCCNHINDGKYFIIQEYGGQLTPFYTTWEMSEEGINNFPCESI
                     EDSISHTEC"
     gene            complement(636..2219)
                     /locus_tag="IXT53_001487"
     CDS             complement(636..2219)
                     /locus_tag="IXT53_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905319.1"
                     /translation="MFILDDEIKKVKNPRIIPLLEEVRSSFYQGNYRSAIAVNYSALI
                     LDLIDKLSDLANIYEDNTSKKILEDIARLRKSDPNSPKWEMELLEKAYSSTELIDSYE
                     YEDLKYIKDQRNYSAHPVVTYNGNTWELRDITKETCQDVIRKSYEIIFLKNPILGKKV
                     TIEIIQYASRIHAMSVTPEEFHSALKNSYLNKLSEKAKENLCKTAFKFVFQLSYGNEE
                     ADRNRESTFRLLNALIFDNKEFYLNILKKEIAKYRFTAESREEINKSLGENQQITYTK
                     GFFLLTFIATHPELQHDFSDETIQNLQISINDFKPKQQVLTEIEYYYRLRSLAVLGNN
                     NEELQNHLNSLVDPILQQKMRVSSLPSSTLETIYNQYLYFGEKEMFIDFMIEHLTDAS
                     SFRLADKDMEVLPWIYNKLNQEQFNKLLYNLNGNNQFYKNSNFPPFISNLLDFYNQKF
                     QVNLRKHYSGLLYPNAVTMDQGFQLKDKELKKLYELAESKSDMYLDIIDNIINKSEDY
                     LRKEINSEKELLNYVHKIK"
     gene            2341..2925
                     /locus_tag="IXT53_001488"
     CDS             2341..2925
                     /locus_tag="IXT53_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905320.1"
                     /translation="MDLKQSFYIWLYPDNPMSIMADIFVVSILLYLFYLIFFHNLIGY
                     MILDWKGMFDIERKPFANNQTYTVFESGDWEENEVDVIHEIGLALSGIQDADVLVSMT
                     TDTGYNAYEVFYLMYFIWRDGQEIINGRAEKTINSNTEISFCMGEGEMIVYKDGSANI
                     KTAFGDIETSIKELREYVQNPYNFQYLAGRINLV"
     gene            2936..3181
                     /locus_tag="IXT53_001489"
     CDS             2936..3181
                     /locus_tag="IXT53_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905321.1"
                     /translation="MKKIINQPTNHLIIEGVIFVTMYFTVGNGISNTIIQKNPQYLLF
                     SLYAWIVQMVWVSILMLLFYWIVRVILLLLNKRKKDR"
     gene            3187..3909
                     /locus_tag="IXT53_001490"
     CDS             3187..3909
                     /locus_tag="IXT53_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905322.1"
                     /translation="MKSTKLKVVLLSTAALITLTACGTTNSANNNKDTKSSSTMVTKN
                     STTSASTTMSSMTENNAEKYQKYEDINKGQKPDEVYNNFINTVKNDENYGNDPLQNKN
                     LTIVLYKPDCPDCQKAESKIMQEIKEGFSYAPTLMHDITINKPAYEYYAVAVDITDGV
                     PEWVQNIPGLADENNQYHTPTVAVVTPKKVNGTLYWDVMGMTTNTTDEMIESTTKYYM
                     DLGAYKLYQQSEPVMDDQSKVK"
     gene            4040..4783
                     /locus_tag="IXT53_001491"
     CDS             4040..4783
                     /locus_tag="IXT53_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905323.1"
                     /translation="MKEISANEAHIPQKNQVGIYKVLHGEGIYKIQLLTGYNNGANGK
                     WLKATSIRELRSEVEKIRLGLTSEEIAKLKGNNSFVIEKEKEKHLLYRELKIEEYYEI
                     DLPEKLRIIDQRSEEKWKTEIYPSLNDYKTSGKPMYNYTGFLTYTQYKYAFRKLKGIK
                     QAEIALYEMKKVDRLKLIQDLVQNTWNQAIVELEKENGKILTNKYLIVNEIEELIGLF
                     SCEKGEVFLKSSPTYSILDSPYQFIQARR"
     gene            4789..5154
                     /locus_tag="IXT53_001492"
     CDS             4789..5154
                     /locus_tag="IXT53_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905324.1"
                     /translation="MSSLKDDYIQAKQEYEKIQMLKSEKVELFLTQKLAEDHIANLDA
                     KTLEEYDRQFNQIHHNIILLVEQAKRRFEKAEYDLLDQGIVCLDKGLRRYYGAELKKQ
                     NVDRNALKHEILELIMQRI"
     gene            5170..5700
                     /locus_tag="IXT53_001493"
     CDS             5170..5700
                     /locus_tag="IXT53_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905325.1"
                     /translation="MSQNISAVKQLIEEDLETTVQRLAERIHFANYQTISNWSSRNRN
                     PEEIPMKFFYRIGKEYGVDPIKIFEKTMIYYYHGTPNEVLEHLLEEAKEKSSILELSQ
                     NDKVIQVKCVDPTGKIIVGFDIERKSKIDFITQLKKINNLDILTLRPDHEQLFMSFKK
                     QLKEIQKRYNKVFPNA"
     gene            5862..6092
                     /locus_tag="IXT53_001494"
     CDS             5862..6092
                     /locus_tag="IXT53_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905326.1"
                     /translation="MWNNRLKTGLLLIVISCAMMIGMRIQREQSYFEVSANNVIEKCY
                     YGQHYWSEEVRENIDREYVQRIVRLPVSELKK"
     gene            complement(6296..6775)
                     /locus_tag="IXT53_001495"
     CDS             complement(6296..6775)
                     /locus_tag="IXT53_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905327.1"
                     /translation="MTDKTSDLPKLAPKTSRVKSKKDGKTKNAVKKKSVKETSSLLLD
                     IPMSSFDDNTDETSVKIATHVGMNNQNDSFYNLLNSLRELEDKKNQVIASKAPDLTTD
                     LDAEIIQIKQDERSVKSLLKTYFDGFDKELAPYSQAILTKPIQAPHISISLQKKSDD"
     gene            6895..8232
                     /locus_tag="IXT53_001496"
     CDS             6895..8232
                     /locus_tag="IXT53_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HAQ4905328.1"
                     /translation="MLMVQFTGIAMSVITINPQVIHADGIATYDAQFNCTTEGSGSGT
                     DSDGNDTGSGAVTGDVAKNIEDIYKYMNGEYGLSGASIAGILGNWKQESGIDPRAVEG
                     YYGDGQREVDFIKQFTDGLMGIGYGQWTAGRHTALVDWSKKNNGGSWWTTEAQLDFMF
                     KGDGGFVGILKDYALNASDDVVENAVTFHKNWEISADSESAVIAVRGENAKEIWKYMK
                     SKGMDGAKDESKINKISGGSSTGDGSNNSSSSASGTVEDACATTDDANSGSGSIGKEG
                     KINGKKGQVIANMTYEEALKKYGKAITLPKFKEPDWSTSPFNGIYQGQCTELTWAYMS
                     QLYDGNQPTTGNGGYVWESYKSAGAKITNKPIVGYGFSASDGWLWAVGDAGHTGVVIA
                     VFEDGSFLCANFNVPPTQAPTRGVSVTLIDGVDGSDNVHFFSGVGKAKFEIEK"
     gene            8255..8782
                     /locus_tag="IXT53_001497"
     CDS             8255..8782
                     /locus_tag="IXT53_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905329.1"
                     /translation="MNSKVKDTIIISLLSGMLLALLVGILFFNKQNQDIEAEKHSVHI
                     EDKLTFFKEVKPNEVDGLRKDLENDIQEMLNGSVQNKFADQSGEEFLRKLFIVTGKPP
                     ITDKSSEKEKIEYYKPFTFKLTNFGIKTSSSGLYEALTDFEVQYNGKKVYDTTLTITL
                     NNDKKVTGGAFYAKS"
     gene            8769..9332
                     /locus_tag="IXT53_001498"
     CDS             8769..9332
                     /locus_tag="IXT53_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905330.1"
                     /translation="MQNHEITTAKKAIGILSIIVILCCVVLFQKKNAYDNNVKELEDL
                     KSKQIEVVKNNQKVKAMQEAQYKELGNAEIKEQSRKFFNLFYNWNSWEKYTKNMKEIQ
                     KEFPQIVKSSPVDISGTMVGTGNSPISSYTADYYTTNQKGKMVELITQTRNTPTTATT
                     TIWRGVTSIGENQLFYLEDLDSYVRAE"
     gene            9755..10492
                     /locus_tag="IXT53_001499"
     CDS             9755..10492
                     /locus_tag="IXT53_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905331.1"
                     /translation="MADRKLEKLLEETWNPKEFSEFFMENFETDLAVIVKDALREQGY
                     PETANYININFTLYTENKGTWDFWATLANKELSDKSDTGIRNFFESNRDDYMYANHQD
                     KLNFRVEFDETPEEFIERQPPKENVAKVLEDRWNSDEIVSTISELGGQYEPLVEAVRE
                     ELRLNKFPDVQNIDVSQIEINVKITNKLDYGSWADIALEKYIYSTLKEFIENRMDIMY
                     LQHPQYLNFGVEIATPLEEWKMEQGLD"
     gene            complement(10509..10925)
                     /locus_tag="IXT53_001500"
     CDS             complement(10509..10925)
                     /locus_tag="IXT53_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905332.1"
                     /translation="MNKKRKLSVLITLVSILTILSGILSLKIFNQINTSSNAKDGIVV
                     ENTSNSKETVTTTKKDKSAEITTKNYLIKTVYFDERKTAILKDEEGSELKLPITVSDK
                     FNGTFSLQTAEGSPDNLKVGQTIILGKNDELYSFVK"
     gene            11317..12564
                     /locus_tag="IXT53_001501"
     CDS             11317..12564
                     /locus_tag="IXT53_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HAQ4905333.1"
                     /translation="MNMKKNSLGMVLFASAVLAGLSVPVTSHADEWTANTPDQIQITT
                     GQSSYAFKKGDTIWSTVEKLKSLGYKTTVAEFAELNNVDLAKGEQRTIPIGRTFNISK
                     DTLTVKEADGEVVSQTVQKDKPDPVENTEQENKKSETAKESEPTANTSDKTETSASSV
                     DGASSSNTNNSTSEKEASQGTSESSTTETDTGTSTNNSQSTSNDANESTSTSSSDQSQ
                     TGSSDETDSSTSPSKPEQPTNVVLGVPFISQGNTMLCEGTSLLEALHYKGVATNQDLM
                     TFVKSMPLSPDNNPNNGFSGEWRHNVDGTYQGMNPAPVVSWGVANGGNVVDISGSSVQ
                     GLKDEIVNGNPVVAWVTYAFEPAQHKSMFWGDAIWNRHVVAVDGFKDGFYHVVDPVFG
                     ASWINAGSFETAYNVSQLAVSVR"
     gene            12834..15224
                     /locus_tag="IXT53_001502"
     CDS             12834..15224
                     /locus_tag="IXT53_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Gln thioester bond-forming surface protein"
                     /protein_id="HAQ4905334.1"
                     /translation="MKHEKTIILKSKIIYCFATLVMVLSTLIMPFSAIRAEADVTKPT
                     SAKFTETGVKTDGYIRVNMPNHPNEWMISSQFKDSHGNIGYCMDSELPSPTGSGAGSL
                     KYKGAGSDEFYRMFKGGFPSKTAKELGAGNDTEAWYATQLVSWVLAGNFKVSQIVWSH
                     PNHTAAETARVKKAFEKIYDYAKNGKDTPNTEFSITASKTADEGKYHTFTYKTASNKT
                     GNAKLTFTSAKPAGMKIYDADGKEITNNTVKLNSSFTIKVPVTTPSGTLSFKGTANVS
                     TTNPFTFDGRGVYQDAVVMITTSETKDSKSLSAKWTRAVGQVTVKKVDQDGNALKGAE
                     FTLTDNNNAKSVKTTGDDGLATFDLQQGLTYSLEETKVPEGYQGSFKKDGITVNTDGQ
                     KFSYTATNTIKKGKVKVKKVDQDGKPLANAEFTLTDDSSQTQAEKTDENGLASFDIVA
                     NKTYSLEETGVPTGYTGAFKQEGITVKDDGQTFEYTATNTLKKGVVKVHKVDQDGKPL
                     ANAEFTLTDEKGATQVEKTDENGLASFNIVANKTYSLEETGVPVGYTGAFKQEGISVK
                     DDGQTFEYTATNNLKKGVVKVHKVDQDGKPLADAEFTLTDEKGVKEVKKTDATGLATF
                     NIIANKTYNLEETKVPTGYTGAFKQEGITVKDDGQVFEYTAKNTLKKGVIKVHKVDDQ
                     GKALANAEFTLTDDKGVKVVQKTDRNGLATFNIVANKTYKIEETQNPNGYTGKWSKEG
                     ITVADDGQTFEYTVINHLVPETVRTGGIGTSAFLLVFLIAGLSMVLLYYREQHKAK"
     gene            15273..17108
                     /locus_tag="IXT53_001503"
     CDS             15273..17108
                     /locus_tag="IXT53_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ4905335.1"
                     /translation="MKSKKIFSNLMLFGTLLGVGLGASSISSSLGLGGQVAYAQQNYN
                     YQGTGTIHIHKVTNTNGDGNPVSDIDNKGKEIALPNGVKPLEGVEFSYVKLTKDEAKS
                     LGWTDSDTTKGQHIDDAKARLLLNKKYSEVSRMKTDSQGNIDIPIQVNTDDLTDNLYL
                     IVETGTVKGVVEEAYPMIVNVPELNGSDDQYFNYDVHVYPKNYFQLNSAELDKKDEDG
                     KALAGTVFELRKDDGSDQVVGTYKTDSTGKLTVDGLELGDYYFLETDLGTDANRTTYL
                     LDGKKFKFSVKRGETAKVVLDVTNYKRPVITKVIDKETVDTGEDVQYTLKPSIPGNIG
                     NYEKYVVKDVLGAELTYDSSEVIEGMTLDTDYSIERDGQTTNYVFTESGMDKLQKQFD
                     AGDRFFTILMNAHTNSKAKMDTPLKNDVSTIYNNGYEPEDETPVDHAYTIIGDHQFIK
                     TDGDTSKALSGAKFVVKNKIDPNKVTYLKQDPTTKAVTWIADKSKATEFVSGSDGKFQ
                     IEGLQYTYDVDFDKQGNPTITDKKINDYALEEVVAPSGYTPTRNDVPFVVSNDDASSE
                     VTDIKNLKTPETVRTGGIGTVVFLVVAGVAGTGLYVVYKREQHKA"
     gene            17183..18040
                     /locus_tag="IXT53_001504"
     CDS             17183..18040
                     /locus_tag="IXT53_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ4905336.1"
                     /translation="MKKKNIKLIILKIGMLLCLGVGLYPVITQAYTYYQTQQTLNSYK
                     EKYDEASEKENEEVFKRYSEVNRKRKDTEQTSVTIPEDNNNIPSNGNLQKDKLGELVG
                     EILIPKINVDLPIYDGVNDFQLSAGAGILTGTDYPTGGIGNHSVISAHSGIPNKKYFT
                     DLELLKLGDKFFIDISGKKLAYQVDRIKTILPTEFEDLLPVAGKDYVTLMTCTPVGDN
                     SHRLLVRGVSIPYQKSIVEKEKKKEDLKVKAQMNWYPIVVVGVLLIIAVIVIIKKIVA
                     KRRGMKNEE"
     gene            18030..19139
                     /locus_tag="IXT53_001505"
     CDS             18030..19139
                     /locus_tag="IXT53_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ4905337.1"
                     /translation="MKNKILLFLTLICTLVLTIYPANQLYHQFKTKENVAEIQEKIEK
                     KQTPSKEEVAKKVEQIKSNSGNQGLDNTQKTDKGVDNTQETDGGSFDIREAEGKVNPY
                     TGDVFSKNQTKDFEPSTYSGAVGYVYIPKINVLELLFLGASQEHLYRGVAQMSATSLP
                     LSGKGHQTVIAGHRGGYTSPQFLYVDQLVAGDHIYIDYLGEKAVYTVFRTDVIRNYGQ
                     NSTLTDIPDLDMLTLYTCHPYPQNYDRLLVRSLRDEKAKYITPQVTVGNTAYADAIDS
                     ESVSDKPETVTSSNDLFEDVDRANKELDGNNDLNIPEQGSVEMQDRTFAEKASSIFTG
                     ITNKHQKMLLVIWTVLGALASLKVLILLIKEFRKA"
     gene            19478..20278
                     /locus_tag="IXT53_001506"
     CDS             19478..20278
                     /locus_tag="IXT53_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905338.1"
                     /translation="MVEISKTALDSLAKLFSSRFDGAEFVEYLSQNESGKTLIETLKK
                     DYLNENDFPTWTNIEDATWDVVCTIYSPWVTIADFVLDKIGGDTYPDAKSLATSDFKT
                     FYGYLMGLIGNGIDLKVTPKQTPEEIEKEKGEVQMAYADDREKVKLLEGKLSEEELGK
                     MVQEINEDSNYFPELKVYQNDEDFFNEMFSNSPWEAVSKTNLGEWNFQDDLVAFSNDG
                     YLRSYSESEYRAELKTYETDILMSYLNLLESGQGELRDKVEELLKEEL"
     gene            20366..21172
                     /locus_tag="IXT53_001507"
     CDS             20366..21172
                     /locus_tag="IXT53_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905339.1"
                     /translation="MRGDQTMSATTDMALEAVIKDNTGNVENVEDFSDIAINLSAWIS
                     AVGEENIEFEIKNMDDLKAKFPFIASKLSQFAEEKSWNELVSFSEKIYDITQEDECVS
                     WEMNNQYKKIDAFVKSRIEGTAFEFEGIDTADNWLNKESFIKSIADDLEKQWSKEDYA
                     EYLKTERDTEVKEKLTEVCLDEHIDPNIVKQEDYEVVIDKVPTDFEEVVKYIVEEALS
                     QYSSDEEVDEETVRTRVWQLVFDDKDYKYLSDQNSVNWSVRLLIDQNDIE"
     gene            21404..22273
                     /locus_tag="IXT53_001508"
     CDS             21404..22273
                     /locus_tag="IXT53_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HAQ4905340.1"
                     /translation="MKKKKFSRSLLGILVLVAVGLSGKGYFQNNSTQEHDFQIKSSVQ
                     VGNKADSESKEFLGIRYDPNKYPNNIVNINDGKYTLTKEDHEALSEKGSQTFWVNYAD
                     LDQLGRAGDVEALITYDAINSHSSKNVERPSFKSSVHIAGEYKDGVFDQERDTWVGKD
                     SNNEITQLEGYKGYLYNKSHSLAWSLGGDMEVHNLTLGTRAQNVGKNDQNGGMAYSET
                     IVRDAIYKNKNEKVLYKVDPIYKGNELVPRGSHVEAFSVNDNGKTVNLNVYVFNNQKG
                     ININYQNGTWVRD"
     gene            22293..22640
                     /locus_tag="IXT53_001509"
     CDS             22293..22640
                     /locus_tag="IXT53_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905341.1"
                     /translation="METISINGLDKSVANRIKKGNGYYLLKKGKYSVARELETMSIVY
                     VAKNHFDVEQAIFDPDSEIGKCVELSEKTVSTKGAMIIEREMLDEIRVIIPKDTQMCE
                     DSISDDEPKSERK"
     gene            22653..23915
                     /locus_tag="IXT53_001510"
     CDS             22653..23915
                     /locus_tag="IXT53_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905342.1"
                     /translation="MSEKSIELKEYKRSDGEKYYPVASFSKSVYLVGRALVVGAIGIY
                     ILHWVTKVVQLLVDTLVSILVSLNDVNGTDDLGLKEMFKELLNAFFPNYLSYSYNVQS
                     FFAKLHLVDISILVLEAGTVFFVGYLVTLMFRHHYGEQRPFFDDMESRRLKREALEAM
                     DVKYDPNKIIGDETNNKKRKRNKTQLESDVLSTLKNIKISVHTRRDLSVNEDIREYIV
                     KIPQPKGKKVRETVLRQIQDLGSILTRATSGRAKFGEKEDEVSGDYFIFRASVNIERE
                     RKERVSTKNKETERATSSIAVSNSQFSFPINLFADNSKNIQNQTEKARKYAETQKQSL
                     ELYLSSADVPASLTDMHVGNTSIEYLFTLSRTRKIQLSSMKEEIETYLHISGVIVRIN
                     AGRIQITLPLGENDRIPIDVPSMINSVF"
     gene            23995..25371
                     /locus_tag="IXT53_001511"
     CDS             23995..25371
                     /locus_tag="IXT53_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HAQ4905343.1"
                     /translation="MAMKLLEKVKKRAEKAESILLDKEPNIKLNGKFSVNMLNTKAKD
                     PTDAIFGIKPDGTIAHFCISEMPHMLVAGTTGSGKSVLLNEILVTVMCHSTPDELKIG
                     IVDPKRVEFGRYKKLPFMLADPITDMDEAYDFFEYLVILMHERYKLMEKAGLQNITLY
                     NEYAEKNGLDRFPYVILLVDEYSQLVGTHKEVEGLIVQLGQMARAAGIHVILATQSPR
                     STVVTGIIKANFPSRVCLMVASDLESRIVLDEGGGESLSPKGDMLIKLVDGSMVRAQG
                     AYISNKEIETIFNHLRNTMPEPEYVDYRAIVAETRGEGGEDEDFGGIGKAPVRKSAKR
                     NLNTPLANPSSKEPSIEKKSAFAIKLEKNKEEKVEQGAKTLEKLQAEKLEKVLRALGK
                     ADPEPQTSNNTNNKQPIASKTKPKANDPKGSENVVITTDKSEKKQINGSTSPIMQKML
                     KAKKQVHK"
     gene            25368..25997
                     /locus_tag="IXT53_001512"
     CDS             25368..25997
                     /locus_tag="IXT53_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905344.1"
                     /translation="MIIVSSFLRWLTPYKYTDEVVVKRAKKRDKKAVLTPKQMLDHGI
                     LGVKVLEVNKPFESNSAREKLLGAEADVAYFYHDKSKHQIAIKFNPKSAWRYQARDDL
                     KRRKLRPMLYPEGKEAFDRTHIVPIGFHGTESDNRLLVGWNSNQNRVQINKFEKKVRK
                     FNDRKTILWFADICKQPDGTAKWEAVIFDENGKKVMSETWHDKAKFVWQ"
BASE COUNT         9700 a         3662 c         5102 g         7587 t
ORIGIN      
        1 aatttcctag gaaaaatagt taacataaaa ggaatgaatt gttatgaaac aatatttaaa
       61 tgaatggaaa gcgatagagg ggtcattagt aacgcaaaga ataaaggaat tacctgattg
      121 cttagagaag gatcatttgt ttcaaatacg agaaatgttg aaaaaagaac agtttgatcc
      181 agaccaattt ttagtagtcg agtatccaac tattggagtg tactgttgta accatatcaa
      241 tgatggaaaa tattttatta ttcaggagta tggaggacag ttaactcctt tctatactac
      301 atgggaaatg agtgaagaag gaatcaataa ttttccatgt gaaagcatcg aagattcaat
      361 ttcacacacc gaatgttaat tgattataac tataaaaaag gattgaacat tatgttcaat
      421 ccttttttca atgaaatgtt ttccgccatt tcattgtttg gtgcgatttc tattttatta
      481 tatcaaaata aagagagatt ctaaagaaag aagagggaaa tatataaatc aagcgacagt
      541 atatttatta ttaaatgcta ttgtgagaat ataactaatt agtttaattt gaaagtaaat
      601 atacgctata aaagtgcatc ttgtgttata aaaaattatt ttattttgtg aacataattt
      661 aacaattctt tctcggaatt gatttctttt cttaaatagt cttcagactt attaataata
      721 ttatctatta tgtctagata catatcactt ttcgattcag ctaattcata cagttttttt
      781 aattctttat cctttaattg aaaaccctga tccatagtca ctgcatttgg atatagaagc
      841 ccactataat gtttacgtaa attcacttgg aatttctgat tataaaagtc taacagatta
      901 gaaataaaag gcggaaaatt actgtttttg taaaattgat tatttccatt taggttgtat
      961 agtaatttgt taaattgctc ctgatttaat ttattgtaaa tccatggtaa tacttccata
     1021 tccttatctg ctaacctgaa tgaactagca tcggtaagat gttcgatcat aaaatctatg
     1081 aacatctctt tttcaccaaa atataaatat tgattatata ttgtttctaa tgttgaagat
     1141 ggtagcgaag aaactctcat tttttgctgg agtataggat caactagact atttaagtga
     1201 ttttgtaatt cttcattgtt gtttcctaga actgcaaggg aacgtaaacg gtagtaatat
     1261 tctatttctg ttagtacttg ttgtttcggt ttaaaatcat ttattgatat ttgtaaattt
     1321 tgaattgtct cgtctgaaaa atcatgttgt aattctggat gggtagctat aaaagtaagt
     1381 aaaaagaacc cttttgtata cgtgatctgt tgattttcac caagtgattt attaatttcc
     1441 tctcttgatt cagctgtgaa cctatacttt gcaatttctt tttttagaat atttaaatag
     1501 aattctttat tatcaaaaat tagggcatta agtagtctga aagtactttc cctatttcta
     1561 tctgcttcct catttccata agataattga aaaacaaact taaaggctgt cttacatagg
     1621 ttttctttgg ctttttcgct tagtttattt agatagctat tttttagtgc agagtgaaat
     1681 tcttcaggtg taacagacat agcatgtatg cgacttgcat attgtataat ttcgatcgtt
     1741 actttttttc ctaatattgg attttttagg aatataattt cataggactt ccgtataaca
     1801 tcctgacaag tttcttttgt tatatctctt aattcccaag tattaccgtt gtaagtgact
     1861 acaggatgag ctgaataatt tctttgatcc ttaatatatt ttaaatcctc atattcatag
     1921 ctatcaatta gctcagttga actataagct ttttctagta gttccatttc ccattttgga
     1981 gagtttggat cactttttct tagtctagct atatcttcta aaattttctt agaagtatta
     2041 tcctcgtaaa tatttgctaa atcagataat ttatcaatta aatctaaaat aagcgcactg
     2101 taattgacag ctattgcaga acgataattt ccctgataaa aactacttct aacttcttct
     2161 aataatggaa taattctagg atttttaact ttttttattt catcatctaa tataaacata
     2221 taaatctcct ttttttattt gtatgacaag tttaccataa aaaaaaatta aaaagcgaga
     2281 catatttatg ttgttgtgtt atcataataa cataaatatg tagagtgaga tgatgaaaaa
     2341 atggatttga aacaatcgtt ttacatctgg ttatatcctg ataatcctat gtcaataatg
     2401 gctgatattt ttgtagttag catcttattg tatttatttt atctaatttt ctttcacaat
     2461 ttaatcgggt acatgattct tgattggaaa ggaatgttcg atatagaaag aaaaccattt
     2521 gctaacaatc aaacctatac cgtcttcgaa agtggagatt gggaagaaaa tgaagttgat
     2581 gtgatccatg aaattggatt agctttatca ggaatacaag atgctgatgt tttggtatca
     2641 atgacaacgg atactggata caacgcttat gaggtatttt atcttatgta ttttatatgg
     2701 cgtgatggtc aagaaattat caatggtcgt gctgaaaaaa caatcaattc taatactgaa
     2761 atttcttttt gtatgggtga aggggaaatg attgtatata aagatgggtc agcaaacatt
     2821 aaaacagcct ttggtgatat tgaaacaagt attaaggaac ttagggaata tgtgcagaac
     2881 ccatataatt ttcaatattt agcaggaaga ataaatttag tttaggagta aaaatatgaa
     2941 aaaaattata aatcaaccaa caaatcactt gataattgaa ggtgttattt tcgtaacaat
     3001 gtattttact gtaggaaatg gcatctcaaa tacaatcatt caaaaaaatc cacaatacct
     3061 attatttagt ctttatgctt ggattgtaca aatggtttgg gtttccatat tgatgctgtt
     3121 gttttattgg atagtacggg ttattctttt attgttaaac aaaagaaaaa aggataggtg
     3181 aataaaatga aatcaactaa attgaaggtt gtattgttat cgacagcagc attaataacc
     3241 cttacagcat gtggtacaac taatagtgct aataataaca aagacacaaa gtctagtagt
     3301 acgatggtta ctaaaaacag cactacatcg gcatcaacaa caatgtcttc aatgactgaa
     3361 aataatgctg aaaagtatca aaaatacgaa gacattaata aaggtcaaaa gccggatgaa
     3421 gtttataaca actttattaa tacagtaaaa aatgatgaaa attatgggaa tgatccattg
     3481 caaaataaaa atctgactat cgttctttac aaacctgatt gtccagattg tcaaaaagca
     3541 gaatcaaaaa tcatgcaaga aataaaagaa ggatttagtt atgctccaac attaatgcat
     3601 gatattacca taaataaacc agcttacgag tattacgcag ttgctgtaga tattactgat
     3661 ggtgtacctg agtgggtaca aaacattcct ggtctggctg atgagaacaa tcaatatcat
     3721 acgccaacag ttgcagtagt aacaccaaag aaagttaacg gcacactata ctgggatgta
     3781 atgggaatga caacaaatac aactgatgaa atgattgaaa gtacaactaa atattatatg
     3841 gatttgggag catataaatt atatcaacaa tcagagcctg ttatggacga tcaatccaaa
     3901 gttaaataga tctttagggc gtaagcccta tttttttaaa attaagtgat aacaaataaa
     3961 tgatttctga aacaaacgtt cttactcaac tactcgaaac acacaagcta attaactaga
     4021 ttataaagga gaatgcgtaa tgaaagaaat cagtgcaaat gaagctcata ttccgcaaaa
     4081 gaatcaggtt ggtatatata aggtgcttca tggtgaagga atttataaaa tacagttact
     4141 taccggttac aataatggtg caaatgggaa gtggttaaag gcaacaagta ttcgtgaact
     4201 tagaagtgaa gtagaaaaaa ttaggcttgg tctcacaagt gaagaaatag ctaaactgaa
     4261 gggaaataat tcttttgtaa ttgaaaaaga aaaggaaaaa catctacttt atcgtgagct
     4321 gaaaattgaa gaatactatg aaattgattt acctgaaaaa ttaagaatta ttgatcaacg
     4381 ttcagaagaa aaatggaaaa ctgaaatata tccaagctta aatgactaca agacatcagg
     4441 aaagcccatg tataactaca caggcttttt aacctacaca caatacaaat atgcctttcg
     4501 caaattaaaa ggcattaaac aagctgaaat tgctctgtat gagatgaaaa aagtggatag
     4561 attgaaattg attcaagatt tagtgcaaaa tacatggaat caagcgattg tcgagctaga
     4621 aaaagaaaat gggaaaattt taactaataa atatctgatc gtaaatgaaa ttgaagaatt
     4681 aattggttta ttttcatgtg aaaaaggtga agtcttcttg aagtcatcac caacctattc
     4741 aattttggac agtccgtatc agtttataca ggcaaggaga tgagttagat gtctagcttg
     4801 aaagatgact atatccaagc aaaacaagag tatgaaaaaa tacagatgtt gaaatctgag
     4861 aaagtcgaat tatttttaac acaaaaatta gcagaggatc atattgctaa cttagatgct
     4921 aaaactttag aggaatatga tcggcagttt aatcagatac atcacaatat cattttgctt
     4981 gttgagcagg caaagcgaag gtttgaaaaa gcggaatatg atctattaga tcaaggaatt
     5041 gtatgcttgg ataagggatt gcgaagatat tatggtgcag agttaaaaaa gcaaaatgta
     5101 gatagaaatg cactaaaaca tgaaatctta gaattgataa tgcaacgaat ttgaagggat
     5161 agaaagcaaa tgtcacaaaa cattagtgct gtaaaacaat taatagaaga agatttagag
     5221 accacagttc agagattagc ggaacgaatc cattttgcaa attatcaaac catctctaac
     5281 tggtcaagca ggaacagaaa tccggaagaa attccaatga aattttttta tcgaatcgga
     5341 aaagaatatg gtgttgatcc gataaaaatt ttcgagaaga ccatgattta ttactatcat
     5401 ggaacaccta acgaagtgtt agaacatctt ttggaagagg caaaagaaaa atcatcaata
     5461 ttagagctat cgcaaaatga caaagttatt caagtgaaat gcgttgatcc aacaggaaag
     5521 attattgtcg gttttgatat tgaaagaaaa tccaaaatag attttattac acaattaaaa
     5581 aagataaata atttagatat tctaacgctt agaccagatc atgaacagtt gtttatgagt
     5641 tttaaaaaac aattgaaaga aatacaaaaa agatataaca aggtgtttcc taatgcgtga
     5701 aaagctcaaa gaaatcagag ttttttggat aattaacaaa gaatgtatca tcttatatac
     5761 tcttggattt cttagcatga tcttgttttg gtggttatat ttaaaacagt aattggagag
     5821 ggtcaagagg atttttatca agataaggat gtggaaactg catgtggaat aatagattaa
     5881 aaacaggatt gttattaata gttatttcct gcgctatgat gattggtatg aggatacaaa
     5941 gggaacaatc ctattttgaa gtttctgcta acaacgtaat agaaaaatgc tattacggtc
     6001 aacattattg gagtgaggaa gtcagagaaa atatagatcg tgaatatgtt caaagaattg
     6061 ttagattacc agtaagtgaa ctaaaaaaat gatgagatta tcagcatatc ttttaatgtt
     6121 tttgaatgtg gtcacgttaa tctttgtagt aatcggtgtg actttcgatt taatggaata
     6181 gtgggaaatc tataatggaa aaaagagaca cgaatgaaca tattttttga atcaatattg
     6241 ttgatggttt ttgcgatcag cgcttaccag aaatctaaaa aagagtaatg attgtttaat
     6301 catcactctt tttttgtagg gaaattgaaa tatgtggagc ttgtattggt ttagttagga
     6361 ttgcttggct ataaggagct agttctttat caaaaccatc aaagtaggtt ttcagtagcg
     6421 atttaactga tcgctcatct tgtttgattt ggataatttc agcatccaaa tcggttgtga
     6481 gatcaggagc ttttgatgcg attacctgat tttttttatc ttccaactct cttaggctgt
     6541 ttaacaagtt gtaaaaagaa tcattttgat tgttcattcc aacgtgagtt gcaattttaa
     6601 cagaagtttc gtccgtattg tcatcaaaag aactcattgg aatatcaagt agtaacgagg
     6661 atgtttcttt tactgacttt ttctttacag cattttttgt ttttccatct tttttgctct
     6721 ttactcgaga tgtttttgga gctagtttag gtaaatcact tgttttatca gtcatttatt
     6781 agcttttcct ttctattaat ttatgttatt atattatcat aataacataa aaaagtacac
     6841 tttttggggg cgtagttatt gaaaaaatct aagagaaaga ttgccacatt gcttatgtta
     6901 atggtgcaat ttacagggat tgcaatgagt gtaatcacaa taaatccgca agtgatacat
     6961 gctgatggga tagctactta tgatgcacaa tttaactgta ctactgaagg tagtgggtca
     7021 ggaacagata gcgatggaaa cgatactgga agtggcgctg taacaggtga tgtagccaaa
     7081 aatattgaag atatttacaa atacatgaat ggagaatatg gcttatcagg tgctagtatt
     7141 gcaggtattt taggtaactg gaaacaagaa tcaggtattg atcctcgagc agtagaaggg
     7201 tattacggag atggacagag agaagttgac ttcatcaaac aatttactga tggtctgatg
     7261 ggtattggct atggtcaatg gacagctggg cgacatacag cattagttga ttggtctaag
     7321 aaaaataatg gcggttcttg gtggactaca gaagcccaat tggactttat gttcaaaggt
     7381 gatgggggat tcgtaggaat attaaaagat tatgctttaa atgcttctga tgatgtcgta
     7441 gaaaatgcag taacttttca taaaaactgg gaaatttcag ctgattccga atcagctgta
     7501 atagctgtac gtggtgaaaa tgcaaaagaa atctggaagt acatgaaaag caagggtatg
     7561 gacggtgcaa aagatgaaag taagatcaat aaaatatccg gaggttcaag taccggagat
     7621 ggatcgaata attcatcaag ttcagcatca ggaacagtag aagatgcctg tgcaactacg
     7681 gatgacgcta attctggttc tggatcaatt gggaaagaag gaaaaattaa cggtaaaaaa
     7741 ggtcaagtaa tagcgaatat gacttatgaa gaagctttga aaaaatatgg gaaagcgatc
     7801 acgctaccaa aattcaaaga accagattgg tcaacttcac cttttaacgg aatatatcaa
     7861 ggtcagtgta cagagttgac gtgggcttat atgagccaat tatacgatgg gaatcaaccg
     7921 actactggaa atggtggtta tgtatgggaa agctacaaat cggcaggagc aaaaataacg
     7981 aacaaaccaa tcgtaggtta tgggtttagt gcttcagatg gatggttatg ggctgttggc
     8041 gatgcagggc atactggtgt agttatagcg gtgtttgaag atgggtcatt tctatgtgct
     8101 aactttaatg ttccaccaac ccaagctcca acaaggggag tttcggtaac tttgattgat
     8161 ggagtggacg gatcagataa tgtgcatttc tttagcggtg taggaaaagc taaatttgaa
     8221 atcgaaaaat aaattggaat gaaggacgat atagatgaat agtaaagtga aagatacaat
     8281 tatcatttct ttgctttctg gtatgttatt agcactgctt gttggaatcc tattcttcaa
     8341 taagcagaat caggacatag aagctgaaaa acacagtgtg catattgaag ataagctgac
     8401 attctttaaa gaggttaaac caaatgaggt ggatggatta cggaaagatt tagaaaatga
     8461 tattcaagaa atgttgaatg gatcagttca aaataagttt gcagatcaat caggtgaaga
     8521 atttttgaga aaactattta ttgtaacagg taaaccacca attactgata agagtagtga
     8581 gaaagaaaaa atcgagtact ataagccttt tacttttaag ctaacaaact ttggaattaa
     8641 gacatctagt agtgggttgt atgaagcatt gaccgatttt gaagttcaat acaatggaaa
     8701 aaaagtgtat gataccacat taaccatcac actaaataat gataagaaag tgactggggg
     8761 tgctttttat gcaaaatcat gaaatcacta ctgctaaaaa ggcgatcgga atactttcca
     8821 taattgtgat cctttgttgt gtggtactat ttcaaaagaa aaatgcttat gataataatg
     8881 tcaaagaatt ggaagacctt aaaagtaaac aaatcgaagt agtcaagaat aaccagaaag
     8941 tgaaagctat gcaggaagct caatacaaag aattggggaa tgctgaaatc aaggaacaaa
     9001 gtaggaaatt ctttaattta ttttacaact ggaattcatg ggaaaaatat acgaagaata
     9061 tgaaggaaat acaaaaagaa tttcctcaga tagtaaagtc tagtcctgtt gatatttctg
     9121 gcacgatggt aggcactggt aatagtccaa tatctagcta tactgctgat tactatacaa
     9181 caaatcaaaa aggtaaaatg gttgaattga ttacacagac aagaaatact ccaacaacag
     9241 caacaacaac aatttggcgt ggagtaacaa gcataggaga aaatcaattg ttttacttgg
     9301 aggatttaga tagctatgta agagcggagt aaaataaaaa aagagctaac ggtaccaacg
     9361 ttagctcttt gtgctgacga tgataacaaa acctaccgaa aaaggattgt ttcatcacaa
     9421 acacaaacag aaaagaattg ataaaaaacc cgaacctcag aatttttatt aatcaattca
     9481 tttagattat aataaattga gctgaacatt acaatacgta tatgttgtaa aacgtaaaaa
     9541 aagtcaaaag taaatacttt cttaaaaagt caaataaaac tattaaagac aatatatatg
     9601 gtttatttga cataagaaaa tggtctagat gataattgta atatagaatt gtaggcttaa
     9661 ataaagcata aaataccttt tatgataagg tatagctctt aattttaagc agtttttaga
     9721 tataagagtg aaagtaaagt gggggagaat aataatggca gatcgtaaat tagaaaagtt
     9781 attagaagaa acgtggaatc caaaagaatt tagtgaattt tttatggaga attttgaaac
     9841 tgacttagca gtgattgtta aagatgctct tagagagcaa gggtatcctg aaactgcaaa
     9901 ctatataaac attaatttta ctttgtatac tgaaaataaa ggaacttggg atttttgggc
     9961 tactttagca aataaggagt taagtgataa atcagacaca ggaattcgta acttttttga
    10021 atcaaacaga gatgattata tgtatgctaa ccatcaagat aaattgaact ttagagtaga
    10081 gtttgatgaa acacctgaag aatttataga aagacaaccg cctaaagaaa atgtagcaaa
    10141 ggtattggaa gatcgttgga attcagatga aatagtttca acaattagcg aactaggcgg
    10201 acaatatgaa ccgctagtag aagcggttag agaagagcta agactgaata agtttcctga
    10261 tgttcaaaat attgatgttt ctcaaattga aataaacgtc aagatcacga ataaacttga
    10321 ttatggtagt tgggcagata tagcattaga aaaatacatc tactcaacgt taaaagagtt
    10381 tatcgaaaat agaatggata tcatgtatct acagcatcca cagtatttaa actttggtgt
    10441 tgaaatagct acacctcttg aagaatggaa aatggagcaa gggcttgact agcctttgct
    10501 ccattttttt attttacaaa tgaatacagt tcgtcgtttt taccaagaat gattgtttgc
    10561 ccaactttga gattgtcagg tgatccttca gcggtttgta aggagaacgt tccgttgaac
    10621 ttatcggata ccgttatagg tagctttaat tctgatcctt cctcatcttt gagaattgct
    10681 gtttttcgtt catcaaaata aacagtcttt attaaataat ttttcgtagt gatctcagca
    10741 gatttgtctt tttttgtagt agtaacagtt tctttagaat tagaggtatt ttctacgact
    10801 attccatctt tggcatttga gcttgtgttt atttgattga atattttcag cgacaagata
    10861 ccgctaagaa tagtcaatat agaaacaagt gtaattaata ctgataattt cctttttttg
    10921 ttcatttttt cttaacttcc tttttgtttt gattagttag atattcctta tctaacaagc
    10981 ctttttaggc tattaaatga agataaaccc caaaaaaata ttgcaattat gacagtgcaa
    11041 tggtactatt attatacaat aaattttgcg aaaggggtag gtctaaatcg caaacaaaaa
    11101 gaaattgaac ctagacaaat acaacttgta caccgcaagg gaagcaagtt tgattttggg
    11161 taaaggtaga aattatgtta caaacataat caactattat ccaaatcgtt tgcctaaagg
    11221 ttcgttcaag aaagtgggtg acaaaaccta tattataacg gaagaaggaa tagaagcatt
    11281 gcgatcacaa acagaaaata aaaacaaaaa gaaggaatga atatgaagaa aaattcgctt
    11341 ggtatggttt tatttgcatc agctgtactt gcggggttat cagtaccagt aacctcacat
    11401 gctgatgaat ggactgcaaa cacaccggat caaatccaaa ttacaacagg acaatcaagt
    11461 tatgcgttca aaaaaggaga tactatttgg tcaactgtag agaagcttaa aagcttaggt
    11521 tacaagacta ctgttgctga gtttgccgaa ttgaacaatg ttgatttggc aaaaggagaa
    11581 caacggacta tcccaattgg acgaactttc aacatcagta aagatacatt aacagttaag
    11641 gaagcagatg gcgaagtagt tagtcaaact gttcaaaaag ataaacctga tcctgttgaa
    11701 aatacggagc aagagaacaa aaaatccgag actgcaaaag aatcggaacc aacagcaaac
    11761 acttctgata agacagaaac aagtgcttca agtgttgatg gagcaagttc atctaataca
    11821 aataattcta caagtgagaa agaagcatca cagggaacaa gtgaaagtag cactacggaa
    11881 actgatactg gaacatcaac aaataactca caatcaacat caaatgatgc aaatgaatca
    11941 accagtacat cttctagtga tcaatcacaa acaggttcga gtgatgaaac tgacagttct
    12001 acttcaccaa gtaaacccga acaaccaact aatgttgtgc taggcgttcc atttattagt
    12061 caaggtaaca caatgctttg cgaaggaaca tctttgctag aagcgttaca ttataaaggt
    12121 gttgcaacaa atcaagattt aatgactttt gttaaatcaa tgccactatc tcctgataat
    12181 aatccaaata atggtttttc tggtgaatgg agacacaatg tggatggaac ttatcaaggt
    12241 atgaatccgg ctcctgtagt ttcatggggt gttgctaatg gcggaaatgt tgtagatatt
    12301 tctggttcat cggtacaagg attaaaagat gagattgtaa atggaaatcc tgtagtagcg
    12361 tgggtaactt atgcctttga acctgctcaa cataaatcaa tgttttgggg cgatgctatt
    12421 tggaatcgac atgtagtagc cgttgatgga tttaaagacg gtttttacca tgttgtcgat
    12481 cctgtgtttg gggcaagttg gattaatgca ggatcatttg aaacagcata taacgtatct
    12541 caattagcgg tttcggtacg ttaataaaag agattgttta tatactaagt agttaagtat
    12601 agtctttatt tgtccttgct taactactta gaaaacttgt tgctcatggt gggtagaact
    12661 aaaaggggtg atgctatgaa tactagagcg ttgaaacagc ttgctttcaa cgaaaaaagt
    12721 gattaatagg tgtggtgatt ttaacttagg aatgggcgcg aaggtcatta ccacaccgac
    12781 atatcgtggc tcattttctt cgcctaactg gtataaagaa ggagtaacga gagatgaagc
    12841 atgaaaaaac gataatttta aaatcaaaaa taatttattg ttttgcgact ttagtaatgg
    12901 tgttatctac gcttataatg cctttttcag caataagagc tgaagcagat gtcacaaaac
    12961 cgacaagtgc aaaatttaca gaaactggcg taaaaacaga cggttatatt agggttaata
    13021 tgccgaatca tccaaatgaa tggatgattt caagtcaatt taaagattct catggaaata
    13081 ttggatattg tatggattca gaattgccat ccccaacagg aagtggagcc ggaagcttga
    13141 agtacaaggg agctggcagt gatgagttct atcgtatgtt taaaggtggt tttccatcaa
    13201 aaacagcaaa ggaactgggt gcaggaaatg atacagaagc atggtatgca acacaattag
    13261 tttcatgggt tttagccgga aactttaaag tttcacagat tgtatggtca catccaaacc
    13321 ataccgccgc agaaacagct cgtgtaaaaa aagcatttga aaaaatttat gactatgcta
    13381 agaatggaaa agatacgccc aacactgagt tttctatcac agcttctaag acagctgacg
    13441 aaggaaaata tcacactttc acatataaaa cagcatcaaa taagactggt aatgcgaagc
    13501 tcacttttac atcagcaaaa ccagctggta tgaagattta tgatgcagat ggtaaagaaa
    13561 ttactaacaa cactgtgaaa ttaaattcaa gttttactat taaagtacct gtgactacac
    13621 caagtggtac tttgtcattt aaaggtactg ctaatgttag cacaaccaat ccttttactt
    13681 ttgatggacg aggcgtgtat caagatgccg tagttatgat cactacttca gaaacaaagg
    13741 actctaaatc tttgagtgct aaatggacaa gagctgttgg tcaggtaaca gttaagaaag
    13801 ttgatcaaga tggaaatgct ttaaaaggag cggagttcac tctaactgac aataacaatg
    13861 ctaaatctgt taagacaacc ggagatgatg ggttagctac gtttgatttg caacaaggct
    13921 taacatacag cttagaagaa acaaaagttc ctgaaggtta tcaaggttca tttaaaaagg
    13981 atggtattac tgttaatacc gatggacaaa aatttagtta cactgctacg aacacaatta
    14041 aaaaaggtaa agtaaaagtt aaaaaggttg accaagatgg aaaaccatta gctaatgctg
    14101 aatttacctt gacggatgat tcatcccaaa cacaagcaga aaaaacagat gaaaacggac
    14161 ttgcaagctt cgatattgta gcaaacaaga cctacagcct tgaagagacc ggagtaccaa
    14221 ctggttatac aggcgcattc aagcaagaag gaattaccgt taaagatgat ggacaaactt
    14281 ttgaatatac agctacaaac actttgaaaa aaggtgtagt aaaagttcat aaagtcgatc
    14341 aagatggaaa acctttggca aatgcagagt tcactttaac cgatgaaaaa ggtgcaacac
    14401 aagtagaaaa aacagatgaa aatggacttg caagcttcaa tattgtagca aacaagacgt
    14461 atagtctaga agaaaccgga gtaccagtag gttacacagg tgcatttaag caagagggta
    14521 tcagtgttaa agacgatggg caaacttttg aatatacagc tacaaataat ttgaaaaaag
    14581 gtgtagtaaa agttcataaa gtcgatcaag atggaaaacc tttagcagat gcagaattca
    14641 ccttaacgga tgaaaaaggt gtaaaagaag ttaaaaagac ggatgcaact ggtctggcta
    14701 cgttcaacat tattgcgaac aaaacttaca acttagaaga aacaaaagta ccaacaggtt
    14761 acacaggcgc attcaaacaa gaaggaatta ccgtcaaaga tgatggacaa gtctttgaat
    14821 atacagctaa aaacactttg aaaaaaggtg tgatcaaagt tcataaagtc gatgatcaag
    14881 ggaaagcatt agcaaacgca gaattcactt tgacggacga caaaggtgta aaagttgtac
    14941 aaaaaacgga cagaaatggc ttggcaacat ttaatattgt tgccaacaaa acgtacaaaa
    15001 tcgaagaaac acaaaaccct aatggttata cagggaaatg gtctaaagaa ggcatcaccg
    15061 ttgctgacga tggacaaacc tttgaatata cagtaattaa tcaccttgta cctgaaacag
    15121 taagaacagg cggaatcggt acatcagcct tccttcttgt atttctaatt gcaggattga
    15181 gtatggtgct actgtattat cgagaacagc acaaagcaaa ataaaaaagt gttaatggaa
    15241 aagaagtaaa tatgaaaaaa gaaggctgac gtatgaaatc aaaaaaaatt ttttcaaatt
    15301 taatgctatt tggtactttg ctaggagtgg gattgggcgc aagctcaatc tcttctagcc
    15361 ttggactagg ggggcaagta gcatatgcac aacaaaacta taattatcaa ggaactggaa
    15421 cgattcatat ccataaagta acaaatacta atggggatgg gaaccctgtc tcagatattg
    15481 ataacaaagg gaaagaaata gctttaccaa atggtgtaaa acctcttgaa ggcgttgagt
    15541 tctcttatgt gaaacttact aaagatgaag ctaaatcctt gggatggacg gatagtgata
    15601 ctacaaaagg tcaacatatt gatgatgcta aagcacgatt attactaaac aaaaaataca
    15661 gtgaagtaag tagaatgaag actgattcac aagggaatat agacatacct atacaagtaa
    15721 atacagacga tttaacagat aacttgtatt tgattgttga aacaggaaca gtcaaaggtg
    15781 tagttgaaga agcgtatcct atgattgtta acgtacctga actaaacgga tcagatgatc
    15841 aatactttaa ttatgatgta catgtctatc ctaaaaacta tttccaatta aactcagcag
    15901 agcttgataa aaaagacgaa gatggaaagg ctcttgccgg aactgttttt gaattgcgaa
    15961 aagatgatgg atctgatcag gttgttggaa cttataaaac agacagcaca ggtaaattaa
    16021 ctgtggatgg tttggaatta ggtgactatt atttcctaga aaccgatttg ggaacagatg
    16081 ctaatagaac aacctattta ctagatggta aaaaattcaa atttagcgtt aaacgtgggg
    16141 agactgctaa agtagttctt gatgttacca actacaaacg acctgtaatt actaaggtaa
    16201 ttgataaaga aaccgttgat actggcgaag atgttcaata tacattgaaa ccatcaattc
    16261 ctggtaatat tggtaattac gaaaaatatg ttgtcaaaga tgttctaggt gcagagttaa
    16321 cttatgattc atcagaagtg atcgaaggaa tgactttaga tactgattac agtatcgaac
    16381 gtgacggaca aacgacaaac tatgtcttta ctgaatcagg tatggataag cttcagaaac
    16441 aatttgatgc aggggatcgc ttctttacaa ttttgatgaa cgctcatact aatagtaaag
    16501 ctaaaatgga tacaccactt aagaatgatg tttcaacgat ctacaacaat ggatatgaac
    16561 ccgaagacga aacaccagtt gatcacgctt atacaatcat tggtgatcac caattcatta
    16621 aaacagacgg agatacaagc aaagcattga gtggagcaaa atttgtagtg aagaacaaaa
    16681 ttgatccgaa taaagttact tacctaaaac aagatccaac aacgaaagct gttacttgga
    16741 ttgcagataa atctaaagct actgaattcg tttccggatc agacggtaag ttccaaattg
    16801 aaggtttgca atatacctat gatgtagatt ttgataaaca aggtaatcca acaatcacag
    16861 ataaaaaaat caatgattat gctcttgaag aagttgttgc tccttcagga tatacaccaa
    16921 ctagaaacga tgttccattt gtggtatcga atgatgatgc ttctagcgaa gtaacagaca
    16981 tcaagaacct taaaacacct gaaacagttc gtacaggtgg aattggaaca gtagtatttc
    17041 tagtggttgc aggtgtggca ggaacagggc tatatgttgt ctacaaacga gaacaacaca
    17101 aagcttaatg aaataaaatt ccctagagtg tttttggctc tagggtttct ttttaaaaag
    17161 tgtgtgaaaa gtaggtatga gcatgaaaaa gaagaatatc aagttgatca tcttaaaaat
    17221 tgggatgttg ctatgtttag gagtaggtct atatcctgta attactcagg cttataccta
    17281 ctaccaaact caacaaacac taaactccta taaggaaaaa tatgatgaag cttctgaaaa
    17341 ggagaatgaa gaagtcttca agagatattc agaagttaat aggaaacgaa aagacacaga
    17401 acaaacctca gtcacgattc ctgaagataa taacaatatt ccgtcaaatg gaaatcttca
    17461 gaaggataaa ttaggtgaat tagtgggtga aattcttata ccaaagatta atgtggattt
    17521 gccaatttat gatggtgtaa acgatttcca actatcagca ggagcaggaa tattaaccgg
    17581 aactgattat ccaactggtg ggattggaaa ccattctgtt atatcagctc attcaggcat
    17641 tcctaataaa aaatatttta cagatttaga gttgctcaaa cttggtgata aattttttat
    17701 tgatatttca gggaaaaaac ttgcttatca agtagatcgg ataaaaacaa ttctaccaac
    17761 cgaatttgag gatttgcttc ctgtggcagg aaaagattat gtaacactta tgacatgtac
    17821 acctgttgga gataattctc ataggttact cgttagaggg gttagcatac cttaccaaaa
    17881 aagtattgtt gagaaagaaa agaagaaaga agatttaaag gtaaaggcgc agatgaactg
    17941 gtatcctatc gtagttgtag gggttttatt aataatagct gtgatagtca ttattaaaaa
    18001 aatagtagca aagagacgag gaatgaaaaa tgaagaataa aatactactt tttttgactc
    18061 ttatttgcac tttggtttta acaatttatc ctgcaaatca gctctatcat caattcaaaa
    18121 caaaagagaa tgtggcggaa atccaagaga aaattgagaa aaaacaaact ccttcaaaag
    18181 aggaagtggc aaaaaaagtc gaacaaataa aatcgaacag cggtaaccaa ggattagaca
    18241 atactcagaa aacagataaa ggagtagaca atactcagga aacagacggt ggatcatttg
    18301 atattcgtga agctgaagga aaggtcaatc catacacagg agatgttttt tcaaaaaatc
    18361 aaacgaaaga ttttgaacca tctacctatt caggagcggt tggttatgtc tatattccaa
    18421 aaataaatgt gctagagctt ttgtttctag gcgcttcaca ggagcattta tatagaggtg
    18481 tggcacaaat gagtgctact agtctacctt tatcaggtaa aggtcatcaa acagtaatag
    18541 caggtcatcg tggcggttat acgtcaccac aatttcttta tgttgatcaa ttagtagccg
    18601 gagaccacat ttatatagat tatcttggtg aaaaagcagt ttatacggtt ttccgaacag
    18661 acgttattag aaattacggt cagaattcaa cactgactga tattcctgat ttagatatgt
    18721 taacacttta tacttgtcat ccatatcctc agaactacga tcgtttatta gttagaagtc
    18781 taagagatga aaaagcaaaa tacatcacgc cacaagtaac agttggcaat accgcatacg
    18841 ctgatgccat tgatagtgaa tccgtttcag ataagccaga aactgttaca tcttctaatg
    18901 atttatttga ggatgttgat cgtgccaaca aggaattaga tggaaataat gatcttaata
    18961 ttccggaaca aggtagcgta gagatgcaag acagaacgtt tgctgaaaag gcaagttcaa
    19021 tatttacagg aattaccaat aaacatcaga aaatgctttt agtcatttgg actgtattag
    19081 gagcattagc aagtctaaag gttcttattc ttttaatcaa agagtttaga aaagcataat
    19141 gaagattata aaaattgata gagtacagta aaaagggatt gatactatta ttgatctctt
    19201 ttttattttg aatagagcta gattcacttt taaataaaat aaaaactgat cttaattcca
    19261 aagaaaaagt caagaaaaaa atacaacaaa tttttttatc agaatattta tcagaatatt
    19321 attagaaaag ataaactcaa aagcttaatt taatagggtt attggttgta tttagaaata
    19381 atgtatggaa aagctataaa gcaatagata agatgtagaa aatcttattt gtgaatgaaa
    19441 aacaagtcat cgtaaaatat taataggagg tgtagtaatg gtggaaatat caaaaacagc
    19501 tttagactca ttagctaaac tatttagtag caggtttgat ggtgcagaat ttgttgaata
    19561 tctaagccaa aacgaaagtg gaaaaactct gatcgaaaca ctgaaaaaag actatttgaa
    19621 tgaaaatgac tttccgacat ggacgaatat tgaagatgct acatgggatg tagtgtgtac
    19681 gatatattct ccttgggtaa cgattgctga ttttgtttta gataaaattg gaggggatac
    19741 ataccctgat gccaaatctc ttgctacaag tgactttaaa acgttctatg gttatctaat
    19801 gggtctaata ggcaatggaa tagatttgaa agttacgcca aaacaaacac ctgaagaaat
    19861 tgaaaaagaa aaaggagagg ttcaaatggc ttatgcagat gacagagaaa aagtaaaatt
    19921 attggaaggt aaattatctg aagaagaact tggaaaaatg gtgcaagaaa tcaatgaaga
    19981 cagcaactat tttcctgaat tgaaggtata tcaaaacgac gaagatttct ttaatgaaat
    20041 gtttagtaat agtccatggg aagcagtgag taaaacaaat ttaggagaat ggaactttca
    20101 agatgatcta gttgcattta gcaatgatgg gtatttacga tcatattcag aaagtgagta
    20161 tagagctgaa ttaaaaacgt atgaaacgga tattctgatg agctatttaa atctattaga
    20221 gagtggtcag ggagagttaa gagataaagt tgaagaacta ttaaaagaag agctataggg
    20281 attatttcaa cataaacaca agcagaaaac taccaaacaa aacgtaccat gcaaaaagcc
    20341 ggttaaaccg gctttttgct ctattatgag aggagatcaa accatgagtg caacaaccga
    20401 tatggcttta gaagcagtaa taaaagataa tacaggaaat gtcgaaaatg tagaagactt
    20461 tagcgatatt gcaatcaatc tttcagcatg gatttcagct gtaggagaag aaaatatcga
    20521 atttgagatc aagaatatgg atgacttaaa agcaaaattt ccttttatcg catcgaaact
    20581 aagtcaattt gccgaggaga aatcgtggaa tgaattagtt agtttttccg aaaaaattta
    20641 tgatattacg caagaagatg agtgtgtttc atgggaaatg aataatcaat acaagaaaat
    20701 tgatgctttc gtcaaatcac gtatagaagg aacagctttt gagtttgaag gtatagatac
    20761 cgcagataat tggttaaata aagaatcctt tattaaaagt atagcggatg atcttgaaaa
    20821 acaatggtct aaggaagact atgcagagta cctaaaaaca gaaagagata cagaggtgaa
    20881 agaaaaactc actgaggtat gtttggatga acatattgat cccaatatcg taaaacaaga
    20941 agactatgaa gtggttattg ataaagttcc tactgatttt gaggaagttg taaagtatat
    21001 agttgaagaa gcattatcgc aatactcttc agatgaggaa gtggacgaag aaactgttcg
    21061 taccagagtt tggcaactgg tttttgatga caaagattat aaatatttga gtgatcaaaa
    21121 ctcggttaat tggagtgtac gattattaat tgatcaaaat gatattgaat aaaatagagc
    21181 tgaaaagctc tatttttttt gataaaaaaa ctagctattt ttttaaaaat aatttggttc
    21241 aaaatttatt tttttaactt gataattttt tgccatcctt ttttaaattg gctatatatc
    21301 aaggtttata gtggggataa ataaaaatac atttgacaaa atttacaata tagtttatta
    21361 tatatttatc ttattatgat aacacaataa aaggaatgat ggtatgaaaa agaagaagtt
    21421 tagtcgatcg ttgttaggaa ttttagtttt agttgcggtt ggtcttagtg gcaagggtta
    21481 ctttcagaat aacagcactc aggaacatga ttttcagatc aaaagtagtg ttcaggtagg
    21541 taataaagca gacagtgaat caaaagaatt tttagggata cgttatgatc caaacaaata
    21601 tccaaataat atcgtaaaca ttaatgatgg gaaatataca ctgacaaaag aagaccatga
    21661 agcattatca gaaaaaggca gtcaaacctt ttgggtaaat tatgcagatt tagatcaact
    21721 tggaagagca ggagatgttg aagcgttgat cacctatgat gctataaatt ctcactcatc
    21781 taaaaatgtg gaacgtccat ctttcaaaag ttctgtccat attgcaggag aatacaagga
    21841 tggagtattt gatcaagaga gagatacttg ggtcggcaaa gattcaaaca atgaaatcac
    21901 tcagctagaa ggatataaag gttatctcta taacaaaagt cattcattag catggtcgct
    21961 aggaggagat atggaggtac acaatttaac acttggaacg agagcgcaaa atgttgggaa
    22021 aaatgatcag aatggaggga tggcttactc agaaacgatt gtacgtgatg caatctataa
    22081 aaataagaat gaaaaagtat tgtataaagt tgatccaatt tataaaggga atgaattggt
    22141 tcctcgtggt tctcatgtcg aagctttctc agttaacgat aatggaaaaa cggtaaacct
    22201 taatgtttat gtatttaaca atcaaaaagg aatcaacatc aactatcaaa atggtacttg
    22261 ggtaagagat tagaaattga gaaggtgaag aattggaaac aataagcatc aatggattag
    22321 ataaatcagt tgcaaatcga ataaaaaaag gaaatggcta ctatcttctg aagaagggta
    22381 aatattcagt tgctagagag ttagaaacaa tgagtatcgt gtatgtagct aagaatcatt
    22441 ttgacgtaga acaggctatt tttgatccag attctgaaat agggaaatgt gttgaattat
    22501 cagagaaaac tgttagtaca aaaggggcga tgatcattga gagagaaatg ttagacgaaa
    22561 tcagagtaat cattcctaaa gatacgcaga tgtgtgagga cagcataagt gacgatgaac
    22621 caaaatcaga aagaaaataa ggagatattc agatgagcga aaaatccatt gaattaaagg
    22681 aatataagcg aagcgatgga gaaaaatatt atcctgtagc cagtttttct aaatcagtat
    22741 atctggttgg acgtgcattg gtagttggtg caattggcat atatattttg cattgggtaa
    22801 caaaggttgt acaactgctt gtagatacct tagtaagtat tcttgtctcg ttaaatgatg
    22861 ttaacggaac agatgattta ggattaaaag aaatgtttaa agagttactc aatgcgtttt
    22921 ttcccaatta tttaagttat tcatataatg tacaaagctt ttttgcaaag ctacacttgg
    22981 tagacatctc tattttagta ttagaagcag gaacggtatt ctttgttgga tatttggtta
    23041 ctttgatgtt caggcatcac tatggagaac aaagaccatt ttttgatgat atggaaagta
    23101 gaaggttgaa aagagaagct ctagaagcta tggatgtgaa gtatgatcca aacaagatta
    23161 taggagatga aacgaacaac aaaaaaagaa aacgaaacaa aacgcaatta gaatccgatg
    23221 tgctatcaac gcttaaaaat atcaaaattt ctgttcatac aagacgtgat ctaagtgtca
    23281 atgaagatat tcgagaatat atcgtaaaaa tcccgcaacc aaaagggaag aaagttagag
    23341 aaacagtttt acgacaaatt caagatttag ggtcgatcct gacaagagca acaagcggac
    23401 gagccaaatt tggtgaaaaa gaagatgaag tttcaggcga ttatttcatt ttccgagcat
    23461 cagtaaatat tgaaagagaa cgtaaggaaa gagtaagtac caaaaacaaa gaaacggaaa
    23521 gagcaacatc ttcaatcgca gtttcaaatt cacaattttc ttttccaatc aatctctttg
    23581 ccgataattc caaaaatatc caaaaccaaa ccgaaaaagc tagaaaatat gccgaaactc
    23641 aaaagcaatc tttagaactt tatctatctt ctgctgacgt acctgcaagt ctaacagata
    23701 tgcacgttgg gaatacatct attgaatatt tgtttacttt atctcgtacc agaaaaattc
    23761 aactaagctc aatgaaagaa gaaattgaaa cttacttgca tatatcaggt gtaatcgtgc
    23821 ggatcaacgc agggcgtatt caaattactt taccattagg agaaaatgat cgtattccaa
    23881 ttgatgtacc atcaatgatc aatagcgtgt tttagaagac atcacaaata gaaacagaaa
    23941 atacaaacta ccagttcaca aacagaaaag taccgagaaa agtgaggaat agaaatggct
    24001 atgaaattat tagagaaagt caaaaagaga gccgaaaaag ctgaatctat tttgctagat
    24061 aaagaaccta atataaagct taatggaaaa ttcagtgtca atatgttgaa cacaaaggct
    24121 aaagacccaa cagatgctat ttttggaatt aagcctgatg gcacaattgc tcatttttgc
    24181 atcagcgaaa tgccacacat gttggttgca ggaacgacag gatcagggaa atcagtatta
    24241 ctgaatgaaa ttcttgttac agtaatgtgt cactcaacgc ctgatgaatt aaaaatagga
    24301 attgtagacc caaaaagggt tgaatttgga agatacaaaa aattgccgtt catgttggct
    24361 gatccaatta cagatatgga tgaagcttat gatttttttg aatatcttgt gatcttgatg
    24421 catgaacgtt acaaactgat ggaaaaagca gggttacaaa acatcacgct atataacgaa
    24481 tatgccgaaa aaaatggatt agatagattt ccctatgtga tcttattagt tgatgaatat
    24541 tcacagttgg ttggaacaca taaagaagta gaaggtttga ttgttcaatt aggacaaatg
    24601 gcaagggcag caggtattca tgttatctta gcaacacaat ctcctcgatc aacagttgtc
    24661 actggtatta tcaaagccaa ttttccgtca cgtgtttgcc ttatggtagc aagtgattta
    24721 gaaagtagga ttgtattaga tgaaggcggt ggagaaagtc taagtccaaa gggagatatg
    24781 cttattaagc ttgttgatgg aagtatggtt agagcgcagg gagcttacat cagtaacaaa
    24841 gaaattgaaa caatcttcaa tcaccttaga aatactatgc cagaaccaga atatgttgat
    24901 taccgagcaa tcgtagctga aactcgtggt gagggcggtg aagacgagga ttttggtgga
    24961 attggtaaag ctcctgtaag aaaatctgct aaaagaaatt tgaacacacc tttagcaaat
    25021 ccatcatcta aagagccatc aattgaaaag aagagtgcgt ttgctattaa gcttgaaaag
    25081 aacaaagaag aaaaagtcga gcagggagct aaaacactag aaaaactgca agcagaaaaa
    25141 ctagaaaaag ttttaagggc attaggaaaa gctgatcctg aaccgcaaac ctcaaacaac
    25201 acgaataata aacagccgat tgcatctaaa accaaaccta aagctaatga tccaaaaggc
    25261 tcagaaaacg tagtgatcac aactgataaa tctgaaaaga aacaaataaa tggaagtact
    25321 tcacctatca tgcaaaaaat gttaaaagca aaaaaacagg tgcataaatg atcatcgtca
    25381 gttctttttt aagatggctt actccatata aatatacaga tgaagtagtt gtgaaacgag
    25441 cgaagaaaag agataaaaaa gcggtactaa ctcctaaaca gatgcttgat catggcattt
    25501 taggggtgaa ggttttagaa gtgaataaac cgtttgaatc aaatagtgca agagaaaagt
    25561 tgcttggagc tgaagccgat gtcgcatatt tttaccatga caaaagcaag catcaaatag
    25621 ctattaaatt caatccaaaa tcggcatggc ggtatcaagc aagagatgac ttgaaacgta
    25681 ggaaacttag accaatgctc tatccagaag gaaaagaagc atttgataga acccacatcg
    25741 ttcccattgg ttttcatggt acagaatcag ataaccgcct attagtaggt tggaacagca
    25801 atcaaaatag agttcagatc aataaatttg agaagaaagt aaggaaattc aacgatcgaa
    25861 aaacgatcct atggtttgca gatatttgta aacagccgga tggaacagca aagtgggaag
    25921 cggtgatatt tgacgaaaat ggtaaaaaag taatgtctga aacatggcat gataaagcta
    25981 aatttgtttg gcagtagcag tagaggtgaa aaagattgct agaacttttt gaagcaaata
    26041 gaacaggctt t
//
LOCUS       DACGLW010000037        26000 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.037, whole genome shotgun sequence.
ACCESSION   DACGLW010000037 DACGLW010000000
VERSION     DACGLW010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.037"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            289..606
                     /locus_tag="IXT53_001513"
     CDS             289..606
                     /locus_tag="IXT53_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905345.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNASIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(697..1062)
                     /locus_tag="IXT53_001514"
     CDS             complement(697..1062)
                     /locus_tag="IXT53_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905346.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            1270..1752
                     /locus_tag="IXT53_001515"
     CDS             1270..1752
                     /locus_tag="IXT53_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905347.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     LVVSRNFPALTVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSMFSLLPRLIIGP
                     NGLSESGLKDSAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            1949..2839
                     /gene="pstS"
                     /locus_tag="IXT53_001516"
     CDS             1949..2839
                     /gene="pstS"
                     /locus_tag="IXT53_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HAQ4905348.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
     gene            3165..5639
                     /locus_tag="IXT53_001517"
     CDS             3165..5639
                     /locus_tag="IXT53_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ4905349.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGHQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKVIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLLKRRNNTVAMTGDGVNDI
                     LAMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLV
                     KNIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNV
                     MEKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKF
                     RSQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLF
                     ERKNEKEQKPFSRVVKKFLSIFKREN"
     gene            complement(5742..7478)
                     /locus_tag="IXT53_001518"
     CDS             complement(5742..7478)
                     /locus_tag="IXT53_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HAQ4905350.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNGDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRAYTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLHGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(7475..8179)
                     /locus_tag="IXT53_001519"
     CDS             complement(7475..8179)
                     /locus_tag="IXT53_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ4905351.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            8310..8813
                     /locus_tag="IXT53_001520"
     CDS             8310..8813
                     /locus_tag="IXT53_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HAQ4905352.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            8773..9111
                     /locus_tag="IXT53_001521"
     CDS             8773..9111
                     /locus_tag="IXT53_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HAQ4905353.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            9132..10550
                     /gene="ffh"
                     /locus_tag="IXT53_001522"
     CDS             9132..10550
                     /gene="ffh"
                     /locus_tag="IXT53_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HAQ4905354.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            10662..11240
                     /locus_tag="IXT53_001523"
     CDS             10662..11240
                     /locus_tag="IXT53_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HAQ4905355.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            11244..11765
                     /locus_tag="IXT53_001524"
     CDS             11244..11765
                     /locus_tag="IXT53_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ4905356.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(11844..12398)
                     /locus_tag="IXT53_001525"
     CDS             complement(11844..12398)
                     /locus_tag="IXT53_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ4905357.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            12651..12926
                     /gene="rpsP"
                     /locus_tag="IXT53_001526"
     CDS             12651..12926
                     /gene="rpsP"
                     /locus_tag="IXT53_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HAQ4905358.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            12938..13189
                     /locus_tag="IXT53_001527"
     CDS             12938..13189
                     /locus_tag="IXT53_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HAQ4905359.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            13313..14104
                     /locus_tag="IXT53_001528"
     CDS             13313..14104
                     /locus_tag="IXT53_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905360.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTATTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            14274..14795
                     /gene="rimM"
                     /locus_tag="IXT53_001529"
     CDS             14274..14795
                     /gene="rimM"
                     /locus_tag="IXT53_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HAQ4905361.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            14785..15546
                     /gene="trmD"
                     /locus_tag="IXT53_001530"
     CDS             14785..15546
                     /gene="trmD"
                     /locus_tag="IXT53_001530"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HAQ4905362.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            15682..16029
                     /gene="rplS"
                     /locus_tag="IXT53_001531"
     CDS             15682..16029
                     /gene="rplS"
                     /locus_tag="IXT53_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HAQ4905363.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            16339..16581
                     /locus_tag="IXT53_001532"
     CDS             16339..16581
                     /locus_tag="IXT53_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HAQ4905364.1"
                     /translation="MNDGKVKQVPSSTKKKNILLKEVLKRFDHGVTYTETEVNSILLN
                     VFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            16932..17186
                     /locus_tag="IXT53_001533"
     CDS             16932..17186
                     /locus_tag="IXT53_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAQ4905365.1"
                     /translation="MLELSKQLPVSDPRHFDYEEIAIKILEELQKNYTTKRVNGSNGL
                     LLHAVYDKNSLKGVDECVIWGDYFYVEGITRLAKTWYCYW"
     gene            17215..18474
                     /locus_tag="IXT53_001534"
     CDS             17215..18474
                     /locus_tag="IXT53_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ4905366.1"
                     /translation="MKRWKKMMIGSIVLGMSLVFGACGNQEDSSEESANEITVSTWNY
                     ETTPEFKALFEAFEKKTGIKVKAVDIASDDYDTKLTTMLSSGDTTDVLTMKNLLSYSN
                     YALRDQLVDQTERIRELDTDAAEETYEMYDIDGKTYALPYRTDFWVLYYNKKMFDDAG
                     IEYPKNLTWDEYEDLAKKLSKNDGQVYGAYQHIWRSTIQAIAAAQNEANLVEPDYQYM
                     TDYYDRALRMQKEGAQMDFGTAKSTKVTYQSQFEEQKAAMMYMGTWYMAGILANKEAS
                     KTEVEWGITAIPQKKKGESVTTFGSPTAFAVNKNSKKQKAAQEFIEFAASEEGAKVLA
                     GVGVVPSYRTDEIDQLYFNLAGMPTDEISKKAFSPDEIKLEFPIDTYGPAIDKILQEE
                     YDLVLVGDETPEKGTVNMEKRVAAEKE"
     gene            18527..19423
                     /locus_tag="IXT53_001535"
     CDS             18527..19423
                     /locus_tag="IXT53_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ4905367.1"
                     /translation="MENSINKKATNHLRRKRTWIAYSFIAPNFIGFFVFTLIPILFSL
                     VLAFMKWDSFSTPEFAGLDNFRKMANDETFWISLKNTFLYTAAVVPGTLVCSLGLALL
                     LNQKIRGMKLFRTIFFFPYVTSLVAIAVVWNMLFHPTVGPINIFLRRFIEHPPGWTSS
                     SNWALWAIVIVSIWRGMGYYMILYLAGLQSIQKELYEASSLDGANRWKQFLNVTLPSL
                     RPTTFFVTIMLVINCFKVFDLVQVMTDGGPGRATNVLVYQIYNEAFKKFDFGYASAIS
                     LVLFVIVLGVTVVQFSWNKRRT"
     gene            19434..20303
                     /locus_tag="IXT53_001536"
     CDS             19434..20303
                     /locus_tag="IXT53_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ4905368.1"
                     /translation="MEESVRNERKKAKIRSKNMPFIQWLGMILLIVLALLTIIPFLWM
                     VSASFKANNEVLTIPIQWIPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFT
                     SSFAVYGFSELNFKGRDILFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQ
                     AFNAFGVFLLKQYYSSIPDSLCESARIDGLSEWEIYRKIILPLTKPAIASLTIITFVN
                     TWNDYMGPFIYLSSTENKTIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQK
                     YFVEGIASSGMKE"
     gene            20313..20988
                     /locus_tag="IXT53_001537"
                     /pseudo
     CDS             20313..20988
                     /locus_tag="IXT53_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
     gene            21194..22858
                     /gene="alsS"
                     /locus_tag="IXT53_001538"
     CDS             21194..22858
                     /gene="alsS"
                     /locus_tag="IXT53_001538"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HAQ4905369.1"
                     /translation="MAEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            22871..23581
                     /gene="budA"
                     /locus_tag="IXT53_001539"
     CDS             22871..23581
                     /gene="budA"
                     /locus_tag="IXT53_001539"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HAQ4905370.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            23711..24208
                     /locus_tag="IXT53_001540"
     CDS             23711..24208
                     /locus_tag="IXT53_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HAQ4905371.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            24393..24653
                     /locus_tag="IXT53_001541"
     CDS             24393..24653
                     /locus_tag="IXT53_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905372.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVIGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(25015..25254)
                     /locus_tag="IXT53_001542"
     CDS             complement(25015..25254)
                     /locus_tag="IXT53_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905373.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            25540..25854
                     /locus_tag="IXT53_001543"
     CDS             25540..25854
                     /locus_tag="IXT53_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905374.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT         8636 a         4373 c         5306 g         7685 t
ORIGIN      
        1 agattacaaa tagtatcgta tttcgcaatc ttaaaattat cttcttactg tgaataagat
       61 aataaaattt gtatttgtcc tttttaggta acttatttta atctatatac ctttctggat
      121 caacgaaagt atactttata tagtcataac gccgatggta actatgcact gtaacgataa
      181 tgctgctaaa atatcttttt acgaaaaaca tcacaaggaa aaacttaact atggtattat
      241 ctaaacataa ttttttcggt atttaatatt taattaggag gtaatatttt gcttttttat
      301 agtaatttta tcttaatagt agcgatatta ttacttctga atatatggat ttttgatagg
      361 agtagaaatg caagtatagg ttttagaact aaacgaagtc tgtcttctaa aaaaaattgg
      421 gtatactcac aaactatttt ttatggagga attgttttaa ttagtttact ttcttcaaca
      481 ctttattctt tgaatattat agatgtttcc acctcaaatt caatatctat tattggaatc
      541 attattgctg caattattac acaactgttt ttagtgtttg gagagaagaa aagaagtaaa
      601 aaatagaaaa actagtgata gttttggtga tataagaaaa acatatatgt ataaaaagaa
      661 gaaagtagct tctctttgta catatatggt ttgtttttat ggatgccgag cttttttata
      721 agataaggct accgcccata acgacccaag gatttgtgga actaagtaaa tacctgcaag
      781 aagcaagatc agctgggtcg gttttaaagg atgatagaag taacgccatt cagtaaaatt
      841 ggcgcaaaat aaataggaag tgacagtaga tataatattg gcaatgaata aagatataat
      901 acgcctgttc ctttgaaaat aatatccagc aaaaagagat aaaaatatca atgtgatgaa
      961 cccgcttacg gaatgataac gaaaatcaag cagtaaggcg aaatgcacat aaggaacaaa
     1021 agataaaagc aacggaaggt ttttccagca agtttttatc atctttcatc accacctaac
     1081 aagtgttatt attttgttag ttttattgta aattgaatcc tgcaataatc aattactttt
     1141 tcattatttc tagattaaat gaataaactc tcatgccatt atctctaaaa aaaaagtttt
     1201 tccccctaat ttttttagat agaaaattgt atagtatgat gagagaaaaa gtagtttggg
     1261 ggaacgttaa tgagaagaaa agcagtaatc gatcggcttt atttattttt ttggtttttt
     1321 cctatcgttt tgctaccttt tacatgggta aatgctgttg tacaaggtgc ggagatatta
     1381 ggcctgatcc aaggaagcgg attagtggtt tctaggaatt ttccagcttt gactgttttc
     1441 tatttagtga taagtgtagg tctcttatta ccaaaatctc tgatcaatca ctacaattgg
     1501 cagctgcttt acttttcttg cttattagta tttacatcta tgttttcttt gctgcctcgc
     1561 ttgattatcg gccctaatgg tttgagcgag agtggattga aggattcagc ttattcctcc
     1621 atgtatttct tgacgatcaa accatggttc tatttagtaa ttgcctcatt tattcttgca
     1681 acattttttt acttctggac agaaatcagg ataaaaagtg aacgatctgt atctataaaa
     1741 aataactatt gatggtggat catcagtaaa aaagcatatg agctgcgtaa aatgcatgtt
     1801 tctcatatgc tttttttatt attatgtaaa gaatgcgaaa aacgtttaca tgatttttac
     1861 ataacttaac tatattttta cagcaaccat acgagggatt tacagtcgac tgataaactt
     1921 tagttgtaaa taagaaagga gcaataaaat gaaaaaagta ttgtttactc taggtatgat
     1981 gggaattctt ttagccggat gcggctcagg atcaggaaat acagatggct ctgccacaca
     2041 aaataattca agtgactcaa atgaacaagt aaaaatcgtt gcggtaggtt cgacagcatt
     2101 acagccgtta gtcgatgcag cacaagaaag ctttgtacaa gaaaatccaa attatcaaat
     2161 ctctgtacaa ggaggaggaa gcggaactgg tttgagccaa gtcgaagctg gagcagttac
     2221 tatcggaaac tctgatgtat ttgcagaaga aagagacgga gttgatgctt caaaattagt
     2281 ggatcataaa gtagcagtgg tcgggatggc accaatcgtc aataaagata cagatgtcaa
     2341 agatatcacc aaacaggaat tgattgatat ctttacagga aaaatcacta actggaagga
     2401 agttggcggg aaagatcaaa aaatcaacgt cgtgaaccgt gcaaacggca gcggtacccg
     2461 cgcgacattc gaaaaatggg gactggatgg tgctacccct gtgcagtccc aagaacaaga
     2521 ttcatcagga acagttcggc agctggtaag tcaaacacca ggagcaatca gctatttagc
     2581 tttctcatat ctcgatgatt ccacacaagc actaagtatc gacggtgtag aaccaaaaga
     2641 agagaatgta gcagataaca gctggggaat ctggtcatat gaacatatgt acacgaacgg
     2701 aaaaccttcc cctgaagtcc aaaaattctt agactacatg atgacagaag aaatccaaga
     2761 aggacctgtc aaagaattgg gctatttacc aatcacaatg atggaagtag aacgtgatca
     2821 cgaaggaaat atcaaataac tagcagataa acatgccacg gtagtcgtta ctattaagga
     2881 aaaatcaact atgtttcccc acatatacat ttttccttta attgtgaaaa atagttcctc
     2941 atattttgcc gttttttgag ctaataccga gggtcagctg attgaacacc gtttattcga
     3001 aaaaaagagc tacgacatag aactgtcgta gctttttttt tgctatatga caggaatgaa
     3061 aggtgtttgc aagcttaatg tgaggaacac tttccatatc tgttctatac tggataggag
     3121 aagtttggaa atatccagaa aacgaaatca atcgaggtag ccacatgaag aataaatgga
     3181 taaatcgaag taaaaaaaag aaagtaacac aaagatcatc agataaagtc atccgtcttt
     3241 ttccatcgta taaagaagga ttaacggcag atcaagtcaa ggaacgaata gaaaaaggtg
     3301 cagcaaataa ttcagtcgat cccacattca aaacaaatca gcaaatcgta ttggaaaaca
     3361 tatttacgta tttcaatttg atctttctta tcttagccat tttgctttgt ttagtagaat
     3421 cctataagaa cttgactttt ttaccagtta tcatagccaa tacaggaatc gccatttttc
     3481 aggaaattcg atccaaaaag atcttggacg aactaaatgt tttaaataca gcaaaagtca
     3541 atgtcatacg agacgggata gaacaaacaa tagatataga agaccttgta atagatgaca
     3601 ttgttatttt agagactgga catcaaattc cagcagatgc agaggtcgtg gaagggaaac
     3661 tgcaagttaa tgaggcttta ttgacaggcg aagcagatga aataaccaaa gaaataggcg
     3721 atccattaat gtccggcagt ttcattgttt ccggaaaagc ttacgcccga ttagacaaag
     3781 ttggagcaga ttcgtatatt tcccagctga cagtcaaagc caagacaatg ggagaaggag
     3841 aacagtcgga gatgattgct tctcttaatc aattgatcaa atggattggg atcatcatta
     3901 ttcctatcgg tatcctgctt ttttctcaaa gttacttttt taatggaaac acgatcaaag
     3961 aaagtgtcgt tgcaatggaa gcagcattga tcggtatgat acctgaagga ctgtacttgc
     4021 tgacgacgat tgcattagca ttaagtgcga ctaaactagc aaagcaaaga gttcttttac
     4081 acaatatgaa aagcattgag acattggcaa gggtaaatgt cctttgtgta gataaaacag
     4141 gaacgatcac agaaaataaa atgtctgttc aaaaagtgat cgtctctaaa aaacaagaaa
     4201 taatcagtca gtcagatcaa ttggaagcat gcattgctga ctatgcaaaa gcgatggcag
     4261 gagacaatgc aacaatggaa gctgttaaag catattttac aaaaaccact gatcaatcct
     4321 atcaccatgt tattcctttt tcttctgtcc aaaaattcag cagtgtagcg ttggcagaca
     4381 aggtgtatgt tttaggagcg cccgaaatgg ttttgcgcga tcaattgccg gaatatgcgg
     4441 aagagtttgt ttctttcgct gaaaaaggct atcgcgtgtt ggtctttggt gtgtacgatg
     4501 ggattcttga agaaccagta ttgacagaag cagtcacacc attaggctat attctgattg
     4561 ctaatcctat cagaaaagaa gcaagagcca catttgatta cttcaaaaaa cagcaagtag
     4621 ccatcaaagt gatttctgga gataatccgc tcaccgtatc caatgtagct gtccaagcag
     4681 ggatttcaca tgctgatcag tacgttgatg catctaaatt atcagaagaa gaatatgaag
     4741 cagctgtcga aaaatataca gtttttggac gcgtcaagcc agaacaaaag aaaatcttcg
     4801 ttcaattgct aaagcgtcgt aacaatacag tagctatgac aggtgatggc gtgaatgata
     4861 ttctagccat gaaagaagca gactgcagta tagcgatggc ttccggaaat gaagccgcca
     4921 tgcaggcctc acaagtggtc ttacttgatt ctgacttttc acgcatgcca gaagttgttg
     4981 cagaaggacg gcgcgttgtg aacaatattg agcgatcagc cagcttgttt ttagtgaaga
     5041 atattttctc ctttttgctc tcactgtttt cagtgatctt tgctctgacc tatcctttag
     5101 aaccatcaca aatcacattg attagtttgt ttactatcgg acttccatct ttcttgcttg
     5161 ctctagaaga aaacaaaaag cgaatcagag ggaaattcat catgaatgtg atggaaaaag
     5221 ctgtgccagg tggactaaca gatatgatcg tagtcggtgc gttggtgatc tgcggggtga
     5281 ctttgaatct aaataaaaca gatgtttcta cagcatctac catgcttttg attgcagtag
     5341 ggttcctcgt tctctataaa atctgttcac cgctgaataa attcagaagt cagatcattc
     5401 ttttctgtgc tagcggtaac tttttttcgg tcatcttttt gcataagctg ttttcaatca
     5461 ccggtatctc agctgtatca cttttgctgt tgagcattct atttttctcg gcagattcga
     5521 tttttaggca tttgaccaca ttggtagaat atttgtttga acgtaaaaat gaaaaagagc
     5581 aaaagccgtt tagtcgcgta gttaaaaaat ttctatcgat atttaaacga gaaaattgac
     5641 gaaaagtatc tcaaaaaaga gtaaaaaaga acaaccactc gtgtatcttt cgaagcagga
     5701 tgagtcatcc aactttaaaa cgcacgagca gttgctcttt tttacatttt cggaatggta
     5761 acggtgaaag tcgaacctgt tccaagatga ctgtctacag taatcgtacc atgaaggagt
     5821 tgcacgtagt cttttacaat cgctaaccct aatccggtcc cgcctgaatg ccggcttcgc
     5881 gccttgtcta cgcggtaaaa acgctcaaag atccgttcct gatcctcaat gtcgatacct
     5941 ataccaaagt cttggacaga aaacgataga tcatctgttg cctgatagcg gataataatc
     6001 tcgctgtcgc tttttgaata ttggacagca ttttcaatca gatttttgat aattggataa
     6061 aggatatctg ttttagtagt aaatggttcg ttctttccaa tcaatcgaat cgtcaaccgt
     6121 ttcttttcga tgattggctg ataattttga acgatttgct ggaaataagg ttctgtataa
     6181 attgtctgga tctcgtaaga attctcgcca tcttttgaca gctggatgat ttcccgaatc
     6241 agtttatcta accggatcgc atctttttgc atgatctcaa gaaaagaagt cagagtttgc
     6301 gggtcatctt ttgcaccatc cagtaaagtc tcggtaaaac caatcagtga agtcacagga
     6361 gtttttaatt cgtgagagac gttgccaacg aaatcctttt gtagcttttc taaacgtcgc
     6421 actttcgtta agtcatacgc cacccctaga atctgtccgg taccttctga attagtaaag
     6481 tagcgcaaac tcaagtctaa tgtctgccga ccttctgtaa gggtgatctc ctgatggatc
     6541 agcggatgct caggagtcac ttgatgaatc agctgaatga gttttggctc ttgaatgact
     6601 tctgtgtacc gctgttcagg ttgatagctt accacgttta aatgtagctg catctttgga
     6661 ttaagcagta gtaaacgaga atcagcatca tcaatcagga atacgccaat catcagttcg
     6721 ttcagtaacg tatataattg ctcttctgtg gtagtatacg cccgatacat cttactcatt
     6781 tgttcgctca acgcattgat cgtctgatac aattcttccc attgatcggg tgtttgcatg
     6841 attacttccg cctgattagg attttgcacc atttttttta gtacgggcag cacggttttc
     6901 aatggctggt ttttttgata gatcagataa tagatcattc ctgcaagaac aaggaagaaa
     6961 gacagaaaga agaaaaagac ccaacggcga aaactctcag ttctcggtaa aaaaccgctt
     7021 gtcggttctg cgatacgtaa aatggcttct aactggccac tttttttgac tggtaatgcg
     7081 acatataaaa gatccccatt caaagtagta cttttgcgca aagcgctacc taggttccca
     7141 ccattcaata cggctttgat ttcgggacgg gagtttcgct gttcattcaa tgtttcattg
     7201 tttgtgtcaa ataagatctt tccagtagca tccattaaag taatccgttc gttcgactga
     7261 tggacgaatt cttccaaagc agttttgttt tgcgctgctt ggaggttttc gacatctaat
     7321 tgccggatca gcagttgtgc tttttttgtc aggtagcttt cttgctgttc cacgatctgc
     7381 tgttgaaaat aatgggaaat caattgccag ccaccaaaaa acaaactaac taaaagaata
     7441 agaaagaaga gacgttcata aatttttttc catttcatcg tttgggttcc tgaaacttat
     7501 agccaaatcc tctaatagtc accaagtact ttgggtgttt tggatctcgc tcgatttttt
     7561 ctcgcaaatg actaacgtgt acatctacga ttcggctttg tcctgcgaag tcaaaattcc
     7621 aaatgcggtc tagcaatgtg tctcggtcga tcacacggtc ttttcgtttc atgaaataaa
     7681 caagtaattc aaattctttc ggtgttagtt caatcagatg gtcgtctacg gtcacttgat
     7741 aattggtcaa atcagcagtg atttgtccaa tcgatagata ttcaggctca gcttcatctt
     7801 ttgattgctt acgaggttca attcgtctga atattgcttt catacgggct aatacttcgc
     7861 gaggactaaa tggtttggtc aaatagtcgt cagcacctat ttctaggcca atgatccgat
     7921 ccacttggtc atctttagcc gtcagcatca aaatcggtgt ttcatttttt tcttgacgca
     7981 gccgctgagt gatctccatg ccatccatcg ttggaagcat cacatctaaa atgatgaaat
     8041 caaatgattg ttctaatgct aattctaatc cttcgcctcc atctgtggca ctggttacct
     8101 tgtagccttc tttttccaaa ttgaacgtca agagcgttac aatagaaggt tcatcatcaa
     8161 caactagtat ttttttcatt caaatctcct ttaaataggt ttcattctgt ttatatttta
     8221 gcatagaatc agtacaaagg gctattttac aaaagaaata aatggtataa taggaaggca
     8281 ctttgctgaa tagaaaggaa gagcagtcaa tgattggaat cagtgcatgt ttaggcggtc
     8341 tggcctgtcg ctatgatgga cagaaaaaag aagtaccaga attgatggaa ctagttgaaa
     8401 ctggagaagc tgtcatggtc tgtccagaag tgatcggcgg attaccaatt cctagagatc
     8461 ccgctgaaat cattggtgga aatggattcg atgtgtggaa agatcaagca aaagtatgga
     8521 cgacaagcgg cgaagatgta acggaagcgt acaaaaatgg cgcgatcaaa gcctatcaaa
     8581 aattgcagga aaaacacatt gatctagtca tcatgaagga aaaaagccct tcatgcggaa
     8641 cccattcgat ttatgacggg agtttttccg gaataaaaaa aaatggtgcc ggagtggcta
     8701 ctgcttattt tattcagcaa aaaatgactg tgctctctga tgaagaatgg ctgaaaacta
     8761 gaggtgagca gaatggaaat cgaaaagacc aatcgaatga acgcgctctt tgaattttat
     8821 tcgaccttat tgacagaaaa acaaatgaac tacatggaat tatattatgc agatgatttt
     8881 tcattaggtg agatcgcgga agaatacgaa gtaagccgac aagcagtata tgacaatatc
     8941 aagcggacaa gcaagatctt ggaggattat gaaaaaaaac ttcatctttt ttctaattat
     9001 attgttcgag aacaggtatt ggaaaatatg actgcatata ttacagataa gtacccggaa
     9061 gataaaaaac tacttgaata tgtacagcaa attcaagcaa tcgaagaata aataaaagga
     9121 atgaagaaaa tatggcattt gaaagcttaa ctgaacgtct ccagcaggcg atgggaaaaa
     9181 taagaaaaaa aggaaaagta tcagaagctg acgtaaaaga aatgatgcgg gaaatccgcc
     9241 ttgccttact agaagccgat gttaacttgc aagtagtcaa agattttact aagcgcgtac
     9301 gcgaacgtgc ggtaggggca gaagtgctgg aaagtttatc acctgcacaa caaatcgtaa
     9361 aaatcgttga tgaagaattg acgatcacac ttggttctga aacagcagag ttaaataaat
     9421 caccaaaaat accaactgtt atcatgatgg ctggtcttca aggggcaggt aaaacgacct
     9481 ttactggtaa attagccaat tatttaaaga aaaatgaaaa tgctcgtccg ctcctaatcg
     9541 caggggacgt atatcgacca gctgcgatcg accaattgaa agtattaggt caacagctag
     9601 acgttcctgt atttgatatg ggaactgaag tcagcccggt agagatcgtc cgtcaaggga
     9661 tggagttagc aaaagaaaag aaaaacgact atgtattgat cgatacggca ggacgtctgc
     9721 atatcgatga aacattgatg gatgagttga aacaaatcaa agaattgacg caaccaaatg
     9781 agattttgct tgttgtcgat gccatgactg gtcaagatgc cgtcaacgta gctgatagct
     9841 tcaaccagca attaggtatt acaggggtcg tcatcactaa acttgatggg gatactcgag
     9901 gcggggcagc actatcgatc cgctcagtga caggcgcacc aatcaaattc atcggttctg
     9961 gtgaaaaatt gactgactta gaagtattcc acccagatcg tatggctagc cgtatccttg
    10021 gtatggggga tatgctgaca ttgattgaaa aagcgcagca agactatgac gagaaaaaag
    10081 cggaagaact tgcacagaaa atgcgggaaa attcattcga tttcaatgac tttattgaac
    10141 agcttgatca agtgatgggt atgggcccac tggaagatct aatcaaaatg atcccaggaa
    10201 tgaatcaagt ccctggaatc gaaaacgtca aggtagatcc aaaagatgtt gaacgcaaaa
    10261 aagcgatggt ttattctatg acgccagcag aaagagcaaa tcctgatctg ttgaacccaa
    10321 gccgtcgtcg ccgaattgct gccggttctg gaaacagtgt tgttgaagtg aatcgaatga
    10381 tcaaacaatt caaggaatca agaaaaatga tgcagcaaat gtccaaaggg gatatgaaca
    10441 tcccaggtat ggaccaaatg tttggttcag gcgtcaaagg aaaactaggc aaaatggcga
    10501 tgaatcgaat gatcaagaaa aataagaaga aaaagaaaaa acggaaataa gatgaaaaaa
    10561 acagcttcgg tacagactga aaatgtctac tgccggagct tttctatcag caatttttat
    10621 tctttactaa tacaattact ctaaacgaag aggtggcaca gatgagatgt ccgtggagtg
    10681 aacaaacaga aagtatgcgg caatatcatg atactgtctg gggtgttcct gaatatgatg
    10741 accaaaagct gttccgaaaa ttgatgcttg atattaatca agccggattg agctggcaga
    10801 cgattttaaa taaaagtgaa gcattcgatg aagcatatga tcgattcgat attaaaaaag
    10861 tcgcaaatta tggagaaaca aaaatcagtg aactaatgga aaacaaagga attattcgta
    10921 accgaagaaa aatcgaagca gccgtccaaa acgccaaagc agtcttgaaa attcaagaag
    10981 aaatcggcag tttttcagag tatctttggt cgttttctaa tggaaaaatc gttgattctt
    11041 cttatcattc acaagaagaa gtcccaacaa caagtcctct ttcagatgaa ttagcaaaag
    11101 atctaaagaa aagaggattt aaatttatcg gaagcacaac aatctatgct tttttagaag
    11161 ctgtcgggat catcaatgat caccttgaca gttgtttccg caaagaagag gtcgtacgta
    11221 tcggcagaaa gggcgaataa agcatgcgaa aaatcatatt ttatggtgct ataagtttag
    11281 atggctattt ggcaacgaaa acggatgatc ttcaatggct ttttgacact ccaacaggta
    11341 agcaaaccac ttatgaagct ttttatgaaa caatcgatac gaccatcatg ggcagaaaaa
    11401 cttatcagga agcaaaaaaa tatttggaga ccgaagcaat ttatccagag aaaaagaatt
    11461 tcgttttttc cacccacgca caattacaat tgccagatgc tgaggtcgta aacgaagatc
    11521 ctgttgcttt tctaaaaaat ctacaaatga cagaaggaaa agatatctgg gttgtgggtg
    11581 gcggtcgatt gatcaaaccg ctattggaac atcaaatgat tgatgaatgg tatatccaga
    11641 ttacccctgt attattaggg gacgggattc ctttattcca aaaaggaaca tatgaagcgc
    11701 gttttgaaat gctagacacg acacgattcg gtgaatttat cgaattgcat tatgtacgaa
    11761 agtagaagta aataaaataa aaaaggcaac tatatgcaga aatgtcctgt acatagttgc
    11821 cttttttatt accgttatgc tttttactct gttaatttgt tagaaaaatc tagatacgca
    11881 tctaaaatcg tttggaagaa acttaatgtt ccttcaacga tattaccatt ttcatcaatt
    11941 agattcgcaa cgtttccgat atatgcttcc ggttgctgca aggttggcac attcaaaaag
    12001 acaagtgatt gtcgtagatg gtggtttgcg ccaaagccgc cgatcccacc tggtgatgca
    12061 gtaacgacta aacctggttt atgatcccaa acactgtgtc cataaggtct tgagcctaca
    12121 tctaaagcat ttttcaatac tgctggcaca gaacggttgt attcaggagt aacgaaaacg
    12181 acaccgtcta gtcccctcac ttcctcacga aaacgagtcc attcagcagg agcctgttct
    12241 ggagtttcga gatcctcatt gtaaaatggc agatcatcga tttttacaaa tacactttcg
    12301 tacccttcag gaagcaattt cgcaaaagct tctgctactt ttttgttgta agaatctttt
    12361 cttaaactac caataaaaaa tccaattttt tttgtcatac gatttttcct ccttcaaact
    12421 atctataaca atcttacaaa aaataagtaa ctggagcaaa taaaaacact tactgacaaa
    12481 agctacatag aagatagatt acttattttt aacaaaaaag aagtctaaaa tcttctattt
    12541 cctaaaaaaa atcgaattta atcaagctgt aaagaaaaaa agctttacag ggtaaaaaaa
    12601 aactgttaca ctacatcttg tgaataaaac aatggaggtg gaaataaata atggcagtta
    12661 aaatccgttt aaaacgtatg ggttctaaaa agagtccgtt ttaccgtatc gtcgtagctg
    12721 attcacgttc tcctcgtgat ggacgtttca tcgaaactgt aggtacttac aatcctttga
    12781 aagaccctgc agaagtagtt ttaaaagaag acttagttct tgactggtta tcaaaaggtg
    12841 cacaaccttc tgacactgta cgtaacatcc tttcaaaaga aggcgttatg caaaaacacc
    12901 acgaagctaa attctcaaag aaataaggtg accggatatg aaagatttaa gcgacttagt
    12961 cttaacaatc gttcgtccgc tagtcactta tcctgatcaa gtcaagttag acgtcgtaga
    13021 gtcgaaagac ttttacgagt acaatctaac agtggctcct gaagatattg gccgtatcat
    13081 tggaaaacaa gggcgtgttg ctaaagcaat ccgcacaatc gtttacggtg ttcggatcaa
    13141 tgcaccgaaa aaagtgcgtt tgaatatcat cgatgataag gaaaagtaaa taaacgtgct
    13201 gtactcgaat tttcgagtgc agcttttttg tttttttatt aattgaagaa atgattattt
    13261 ggcaaaacaa gcaagaaagc gtatccttat aggaggaggg agtgaacagc caatgagaaa
    13321 gaaaatgtta tttattccat ttgctctatt gttaagcgct tgtagttcgg ttactcaaga
    13381 atcagacaat acacatctat caagcagtat atctgaaact gttaccagtc cagaaaaaag
    13441 tagtaccaaa acggctacca ccactgaaca aacaatggct acctcaaata atgagaaaaa
    13501 gactgcttta gaccagttga aagaacaaca gccaaatgtt cccatgcctc tagatgtacc
    13561 agtctcttca ggctatctca atatcgcagc gacacataca aagcaaggtt attctatttt
    13621 atattacaga acagatcgtc ccctcgggtt gaatgctgac gaattgaatc aagaaacgcc
    13681 tattgcaacc tatctatatc aatatgggtt tgcttctagt caagaaacaa tccaagtttt
    13741 gcagcccttt gagatcgaca caaatggtca gcaagtggac ttaggttctc gaatcacagg
    13801 gtatcagcaa ggtgcagcag gttcaagttt tcttgaatgg caagaaggaa actggcgtat
    13861 tcgaatccgt gggaataata tagaaggcca agatccctta ctattagcta aagaaatcgt
    13921 agcttatttg gaagaaaaca gtctaccagc acctgaacaa tttggtaaaa ttactgttga
    13981 tatgggtgat acgacgaatc gagccgtaga agtaagctgg caagaaccga aaaatgccta
    14041 tacgatcact catcaagatc ctatgtctgc gttgaagatg gcagtttcta tgaagaggtt
    14101 gtgaaatcag cgcgctgatc tgagcccatc atttaactcc aagtcacagg aagtaatcaa
    14161 aaatagcacg ccgcattttt gcataatttt ggcttaatac cttaaaagaa aactagaatt
    14221 gcctcttcac acatgctaaa ataaaacaga caaaaaagaa atggggatat aagttgactg
    14281 aatacttgaa tgtaggaaaa atcgtaaaca cacaaggtat caaaggcgaa gtccgtgtta
    14341 tctcaacaac ggatttccca gaagaacgtt acaaaaaagg aacggtgtta acactattcc
    14401 aagacggaaa agcacctgtg gaattaacag tcaaaagtca tcgcaaacat aaaaattttg
    14461 acttattgag ttttgaagga catccttcta tcaatgatgt ggaaaaatat cgggatggca
    14521 tattaaaagt gtcaaaagac aactctgtag atctagctga aaatgaattc tattaccatg
    14581 agatcatcgg tctgcatgta ttagaagatg gaactgagtt aggtaaagta aaagaaatct
    14641 tgtcaccagg tgccaatgat gtttgggtcg ttcaacgtcc gaaaaaagct gatatattgc
    14701 ttccatatat cgcttctgtc gtcaaagaag tcgatttaga agctggagtg gtccgtgtag
    14761 aaattccaga aggactgatt gacgatgaag attgatgtat tgactttatt tccaagaatg
    14821 tttgaaggtc ctatggggga atccatcatc ggtaaggctg tagataaagg attattagat
    14881 atcaatatct ctaacttccg cgattattca gataacaaac atcagacagt agatgattat
    14941 ccatacggcg gtggagcagg tatgctgctc aaagtccagc ccatctatga taacatccaa
    15001 gccattgaaa aagctgcacc ggatgtcaaa aaaagagtaa ttcttcttga cccagctggt
    15061 aaacgattcg atcagaaaat ggcagaagaa ttttcacaag aagaacactt agtattcatt
    15121 tgtggtcatt atgagggata tgatgaacgt atccgcacat tagtgacaga tgaagtttcc
    15181 ttaggagatt atgtccttac tgggggagaa ttaggtgcga tggtcatgat cgatgcaaca
    15241 gttcggcttt tgccagatgt gttagggaat cagacttcag ctcaaacgga ttcttattca
    15301 accggtttat tggaacatcc ccaatatact cgcccggcag aatataaagg gatgaaagta
    15361 ccagaagtat tgacaaatgg aaatcataaa ttgattgaag aatggcaatt aaaagaatca
    15421 ctaagaagga cctatcaacg tcggcctgat cttttggaaa cacttgaatg gacacctcaa
    15481 atgacgaaat ttttagaaga aatcaagaaa gaagaacaag aaaaaacagc agatgaagcc
    15541 aagtgacttt ttctaaaata ttctttacag taggttgata atatgttagt attttatggt
    15601 gagtgcaaaa gcacttaact attacgatat tccgctatga gaaaagctca taagaatatt
    15661 tggaataagg agaagaaaac catgaatcca ttaatcgaag aattaacaaa agaacaatta
    15721 cgttctgaca tcccagcgtt ccgccctggt gacactgtgc gtgttcacgc aaaagttgtt
    15781 gaaggtactc gtgaacgtat ccagttattt gaaggtgttg ttatcaaacg ccgcggtgct
    15841 ggaatcagcg aaacttatac agtacgtaaa gtttctaacg gagtgggcgt tgaacgtaca
    15901 ttcccattgc atacaccacg tgttgcaaaa atcgaagtag ttcgctacgg taaagtacgt
    15961 cgtgcaaaat tgtactacct acgtgcatta cacggaaaag ctgctcgtat caaagaaatc
    16021 cgtcgttaat cattgttatc agactgagaa agaacccttg gtattcaagg gttctttttt
    16081 cttagcatgt aatttgaaca ccctgatcca gtttttttct ttaattattg gtaggggaga
    16141 ataatggatt agaataaatc tacctataag tagtagtaaa aaaaagagca ctatgataat
    16201 atctttctta agagtaaccg ctttaataaa aaatccagaa tattctaaca taaatggagt
    16261 attcttagaa tcaaaaggag aaagaagatg gtttatactt atacggagca agaaaaaatg
    16321 aaagtttgag acacattcat gaatgatgga aaagtaaaac aagtaccaag ttcaacaaaa
    16381 aagaaaaaca tcctattgaa agaagtcttg aaacgctttg accatggagt gacttataca
    16441 gaaacagagg ttaattcgat actgctgaac gtattttcta gtggcgatta tgtcgaacaa
    16501 agacgttatc taatcacatt tggctttttc aaaagaagtt cagacggtcg ggcatatcag
    16561 atgatgggaa ttgaaaatta gaagttttaa tttatactca ttgaaaaaag ttcaagtatg
    16621 cgatattggt tctacaccgc atacttgaac tttttttgtc atctgtatct ataatacaaa
    16681 gccaagaacg ttattcaaaa tatggatagc tatgataaaa gctaatgagc gattcgcagt
    16741 taaataagca atcgtcagta aaatcgttcc aggagcgtac atgaaaaaat caattggatg
    16801 cgttacatga ataaagataa ttctctttta tttgtacgaa taaataccaa aacgtacctc
    16861 ctaaaattag attcaaagaa gatagcaaag aggaaaaaga tagttctgca gcagctatag
    16921 cggcatgtgg attgctagaa ctttccaaac aactgcctgt ttcagatcct cgtcatttcg
    16981 attacgaaga gatagcaatc aaaatcttgg aagaattaca gaaaaactat acaacgaaac
    17041 gagttaacgg atcaaacggt ctgcttctcc atgccgttta tgataaaaac agcttaaaag
    17101 gtgtagatga atgtgtgatc tggggcgatt acttttatgt agaaggaatc actcgtctag
    17161 ctaaaacatg gtactgttac tggtgatgta taagagaaga ggaggattca tataatgaag
    17221 cgatggaaaa aaatgatgat tggatcaata gttttaggaa tgtcactcgt atttggtgct
    17281 tgtggtaatc aagaagacag tagtgaagaa tcagcaaatg aaattaccgt ttcgacatgg
    17341 aattatgaaa caacacctga attcaaagcg ctctttgaag cattcgaaaa gaaaacggga
    17401 atcaaagtga aagcagtaga tattgcttca gatgattatg atacgaaact caccacgatg
    17461 ctttcttctg gagacacgac agatgtattg acgatgaaaa atcttttgtc gtattcaaat
    17521 tatgctttac gagatcagtt agtcgatcaa acagaacgaa ttagagaatt agacactgat
    17581 gctgctgaag aaacatatga aatgtatgat atcgatggaa aaacatatgc attgccatat
    17641 cgaacggact tttgggtact atattacaat aagaaaatgt ttgatgatgc aggaatcgaa
    17701 tatccaaaaa atctaacttg ggacgaatat gaagatttag caaaaaaact tagtaaaaat
    17761 gacggacaag tttacggagc atatcaacat atctggcgtt cgacaatcca agctattgca
    17821 gctgctcaaa acgaggcgaa tcttgttgaa ccagattatc aatatatgac tgattattat
    17881 gatcgagcat tgaggatgca aaaagaaggc gcgcagatgg attttggcac ggctaaatca
    17941 acgaaagtaa cctatcagtc tcaatttgaa gaacaaaaag cagcaatgat gtacatggga
    18001 acttggtata tggctggcat actcgcaaac aaagaagcaa gtaagacaga agtagaatgg
    18061 ggaattacag ctattcctca gaagaaaaaa ggagaatcgg tcacgacgtt tggctcacca
    18121 acagcatttg ctgtcaataa aaactcaaag aaacaaaaag cggcgcaaga atttattgaa
    18181 tttgcagcca gtgaagaagg agcaaaagta ttagcaggag taggagtagt tccttcatat
    18241 cgtacggatg agattgatca gctttatttt aatctcgctg gaatgccaac ggatgaaatt
    18301 tctaaaaaag ctttttctcc tgatgaaatc aaacttgaat ttccgattga tacgtatggg
    18361 cctgcaatcg ataagatttt acaagaagaa tatgacttag ttttggttgg agatgaaacg
    18421 ccggaaaaag gaacggtaaa tatggagaaa cgtgtagctg cggaaaaaga ataatcatga
    18481 aaggagagaa gccaagagat aaaacactct tggtttcgtc atcgaaatgg agaatagtat
    18541 aaataagaaa gctacaaatc atttgcgtag aaaacgaacg tggatcgctt attcgtttat
    18601 cgccccgaat ttcattggtt ttttcgtttt tacattaata ccaatcctgt tttcgcttgt
    18661 actagcattt atgaaatggg attctttttc aacgccagaa tttgctggac tagataactt
    18721 tcgtaaaatg gccaacgatg agacattttg gatttctttg aaaaatactt ttttatatac
    18781 cgctgctgta gtgcctggca cacttgtttg ttcgttaggg ctagctttac tactaaatca
    18841 gaaaattaga gggatgaagc tttttcgtac gatatttttc tttccgtatg ttacttcctt
    18901 agtagcaatt gctgttgttt ggaatatgtt gtttcatcca acagtgggac ctattaatat
    18961 ctttttaaga cgatttattg aacatcctcc aggttggact tcaagttcaa attgggcgct
    19021 ttgggcaatt gtaattgtca gtatttggcg agggatgggt tattacatga ttctatacct
    19081 tgcaggacta caaagtatcc aaaaagaact atatgaagcg tcttcgctag atggagcaaa
    19141 ccgttggaaa caatttttga atgtgacatt accttcgttg agaccaacaa ccttctttgt
    19201 aacgatcatg cttgtcatca attgtttcaa ggtgtttgac cttgtgcaag tgatgacgga
    19261 tggcggccct ggtcgagcga caaatgtgct ggtttaccaa atctacaatg aagcattcaa
    19321 aaaatttgat tttggttatg cttcagctat ttcacttgtt ttgttcgtaa ttgttttggg
    19381 cgttacggtt gttcaatttt cttggaataa gcgtcgtact taggaggtgt gatatggaag
    19441 agagtgttcg aaatgaaaga aaaaaagcaa aaattcgttc aaaaaacatg ccctttattc
    19501 aatggttggg tatgattctg ctgattgtat tagctttatt gacgatcatt ccttttttat
    19561 ggatggtttc tgcttcattc aaagcaaata atgaagtgtt aacgattccg attcaatgga
    19621 tccctaaaac ttggcattta gagaattatc tggttatttg gtctcgtatt cctttagtga
    19681 cattttttaa gaatacagct tttttaagcg ttgtgatcac gttgattcag ttgtttactt
    19741 ctagttttgc tgtttacggg ttttctgaac taaattttaa agggcgcgat attttatttt
    19801 tagcatatat cggcacgatt gctgtccctt ggcagtctta tatgatcccg cagttcatta
    19861 tgatgagaaa agtgggttta acagatacgt tgtggtcgtt ggtgctatta caagcgttca
    19921 atgcttttgg tgtgttcttg ttgaagcaat attacagtag tatcccagat tctttatgtg
    19981 agagtgcgcg gatcgatggc ttatcagagt gggaaatcta tcgcaagatc attttacctc
    20041 ttacaaaacc ggcgattgcc agtttgacga tcatcacatt cgttaataca tggaacgatt
    20101 atatggggcc attcatttat ttgtcttcta cggaaaataa aacaatccag ttagggttga
    20161 aaatgtttat tgggctattt gatgcagagt acgcattaat catggctgcc tcagttgttt
    20221 ctgttcttcc tgttgctttt atatttttag cgatgcaaaa atattttgta gaagggatcg
    20281 cttcatcagg aatgaaggaa taggaggtga ttatgaaaga gcagcgtttt gacaaaaatg
    20341 tttatatgga attcttttat tggttgtatc aatcattggc aagtagcctt tgtttttggt
    20401 gtgtcaatat cttgtatata gcagctgcgt tccttttgtc gatagatgga aggaatgtct
    20461 tttcctttat tttttcttta ctttttattg gtccaggttg catcgtgatg ataggtatga
    20521 cagaagaatt agcaaaagaa aaaagttttt caccagtacg tttgttcata aagacgtttc
    20581 gccaattttg gctaaaggga tttttttatt ggctatttgc ttggatagtt agtgtgataa
    20641 tgatttttga ttgtttcttt tttatacgtt tttcttatgg aaaatggttg attccgttgt
    20701 ttgttttact tgcttgtttt acttgcttgt ttatcagttt cctttagcat caactgctgg
    20761 tattttcagg tgagaaatcc agcgtcgaaa ccaaatcaag ttctacggat agcgttctac
    20821 tatacgttga aaaaatggta tgtgagttta ctagattttt tactattgac aagtttgttt
    20881 ctcttctttt tcgtaaagcc ccaatggtgt attctactag gtccttctat cgtatttggt
    20941 ttgatttact tcaacaatcg taaattgatg agaactatgg atctgtagac tttataaatt
    21001 gaaactttca attaagaatg acagtttttt atgtaaaaac tgtcattctt tttcattttt
    21061 tgaaacatat ggctaaacaa atgaaataaa acatgctata cttttgaatg aaacaggttt
    21121 agaaactgta tcacttattt gtgattattt ctaactaata attaaaaata aagcataaaa
    21181 aggacgatgt tcaatggctg aaaaaatgaa acaaggttcg aaatctgtag tagaaagttt
    21241 gattaatcat catgttgatt atgtattcgg tataccaggt gcaaaaatcg atggtgtatt
    21301 taatgaatta gaggatcaag gtcctgaatt aatcgtgact cgtcacgaac aaaatgctgc
    21361 atttatggca caagcaatcg gacgtattac aggagagcca ggagtagtta tcgcaacaag
    21421 tggtcctggt gctagtaact tagctacagg tttggtaaca gcgacagcag aaggtgaccc
    21481 agttttagca attgccggac aagtaaaacg aagcgatcta ttgaagttga cccatcaaag
    21541 catggataat gcagcactgt tccagccaat cacaaaatac agtgcagaaa tccaagatcc
    21601 agaaacaatt tcagaagtga ttgccaatgc ttatcgtatg gctaagagtt caaaaaaagg
    21661 cgcgagcttt atcagtatcc cgcaagatgt agtggatgca cctgtccaag gtaatgtcat
    21721 caagccattg agcgatccta aattaggttc tgcttctgca gatgatatcc gttacctagc
    21781 tgaacgcatt agagaagcaa aactaccggt actattagta ggtatgcgag gatcaagtga
    21841 aaaagaaacg ttagccatca ggcaattagt tgaaaagaca gcattgcctg ttgtagagac
    21901 attccaagca gctggagtga tctcacgtga attagaagcg catttctttg gtcgtgtggg
    21961 tttatttaga aaccaaccag gagatatgct tttgaaacga agtgatctag ttattgcaat
    22021 tggctatgat ccaatcgaat atgaagcaag aaactggaat gcggaaaaag atgcgcggat
    22081 cattgtgatc gatgaagcgc cagctgaaat cgatcctttt atgcagcctg aaagagaatt
    22141 gatcggtgat atctcagcca ctcttgattt attgacaggt tcattagagc ctcaacaagt
    22201 gtcagaagat gcaaaagaat atttagcatc tcttcaagca aaattaacgg agcgggacat
    22261 tgttcaatca aaaggagaag caggtatctt gcatccttta gaagttatta acacgcttca
    22321 gtcaaaagta acagatgata tgactgttac cgtagatgta ggtagtcatt atatttggat
    22381 ggcacgccac tttagaagtt atgaacctcg tcatttgtta ttcagtaatg gtatgcaaac
    22441 gcttggtgtt gcattgccat gggcgatttc tgctgcattg gtgcgtccaa atacacagat
    22501 cgtatcagtt tccggcgatg ggggattttt attctctgcc caggatttag aaacggcggt
    22561 acgtaaaaaa ttgaatatcg ttcatttgat ttggaatgat ggacactaca atatggtaga
    22621 gttccaagaa aaaatgaaat atcagcgggc gtcaggtgta gactttggtc cagttgattt
    22681 tgtgaaatat gcagaagctt ttggcgctaa aggaatccga gctacaagtg tagaggaatt
    22741 agaaaaagct ttagaagaag gatttgcgac agaaggaccc gtaattattg atattccgat
    22801 tgattaccgt gataatgaga aattgggaga aacaatctta ccagatcaat tctattaagg
    22861 aagtgtcgac atgactgaaa aaatcttata ccagcacggg acattaggag ctttgatggc
    22921 cggcttgatg gatggaacag aaacgattgc tcatatatta gaaaaaggaa ctctgggatt
    22981 gggaacattg catgggcttg atggtgaagt catcttttta gatggcgtag cttatcaagg
    23041 aagatcagat ggctcagttg cccaattaga tggaagtgaa cttaccccct atgcagcaat
    23101 tacagatttt acgccagata ctagtttttc tgtctcaaaa actgcagatg gagaacaatt
    23161 gaaaaaagac atattagtaa gagaagccgg ggaaaaccta tttttggcag tgaagataac
    23221 tggattattc aaaaatatgc acatccgtat catgccgaag caagaaaaac cttacagaag
    23281 actagcaaaa atttcagagt ctcaaccaga attccaacaa agcaatgtcc aaggaacatt
    23341 agtcgggttc tttacgcctg aattattcca aggagttgct gcagcgggat ttcacctgca
    23401 ttttatcgat gatacacata catttggcgg acatgtaatg gattttgaag tggctgaagg
    23461 aaaagttgag attagccgca tctcgtccct tgtacagcat tttccagttg atgatccgac
    23521 ttttgttcac gaaacgattg attatgctga tttagcagca gaaatccaac aagcagaata
    23581 gaaaaagaca gcgagatgcg ttcaaggtga ttaacctgac gtatctcgtt tttttgagga
    23641 gaaaacctag cgtttacaaa aaaactgagc tataatcttc tccatagatc aaaaaggagc
    23701 ggtttttatc atgtggacag aaaaacagaa aaaagatgca tacgaaattt tattattaca
    23761 gcaacaagga ttattagaag cagaagataa ctggttagca aatttagcaa attcatctgc
    23821 attgttgaat gagactttgc ctgagactgt ctttgcagga tattatttat tcgatggtaa
    23881 tgaattgatt ttaggaccgt tccaagggcg agtttcttgc acacgtatca agatgggaaa
    23941 aggagtttgt ggagaatcag ctgaaaaaca agaaacattg atcgttgatg atgtcaaaac
    24001 ccatgcaaat tacatttcct gcgattcagc cgcacaatca gaaatcgtag tgccaatggt
    24061 aaaagaaggt aatttaatcg gtgttttaga tatcgattgc ggagtaacga aagggtatga
    24121 tgaaatcgat caagcatttc tagaaaaata tgtagatctt ttattagcaa attttaatat
    24181 aaatgctttt tcatttatta ataactaaaa tcgtctattg aaacaaaaaa gaagagaaat
    24241 caagaataaa aagagccgga aagacagtgg gcatttctgt ttttcggttt ttttattttt
    24301 ttataaaatc acaagtgtta ttgattttca taatcatatt ttaatgttat tatcatttta
    24361 aataatgaaa aaacattaag gatggatgaa atatgaataa cgaaactatc attttaggaa
    24421 aaaaatatat ttgccaacct attggcttga aacaaaaagt gattggagaa gtaatcagta
    24481 aattagaaaa ttgtgttgtg atctgcgtag acgaatatca tgacgtcgat cgcaatgaaa
    24541 tattagaaaa atgcggaaaa gtagtggcaa aatatactca cgtttatgag ttggaaacaa
    24601 cagaatatct atataactat agcaacaaat atgaacatac tttagtttca taaagattta
    24661 aataaagtgc cttcaaaagc gagaagacca agcttttgaa ggcactttat ttagttggca
    24721 gtgattgttc atgatttttg atttattcaa aaggttcgcc tagagaatct gtctctgtaa
    24781 cgaggcctgg tggagaaact cgataaaaac ctatactgcc agaactgtcc tattttcgga
    24841 tagacaaact agtagtttta aaaagatcat ccgatccaat catttcaaaa aatgcaaaac
    24901 tgatatcagg atcatttagc tcaggcttgt tatcgtaaag catttgtaaa ggttgcattt
    24961 agttaggagt aaacgcgaaa ctatcatcat tatttgctga agaagatgaa gaagctaatt
    25021 cagagtttgt ttgaaataaa ggagacgtgc tgacagtttc tttaccctgt tttttgcaga
    25081 ggtagaaact tttatatcgg aagtttctac cggagaattt ttctcatttg tagaaattgt
    25141 tgaatcgata ctattcgaaa tttgcgtatc tgaaggtatg ttctttgatg cagaagtccc
    25201 acaagcagta agtaacaata aatttgataa actgataaac agaattcttt tcattatgta
    25261 cacactcctt atagtatcgt ttagcataca cctatgctca gtattaggca aaatcaatgc
    25321 aaaactatca agtcttttat agtatctatt agtcttagta atacatatat ggagagggtg
    25381 atatcccagt tcaaaccaga tacattggtt accatctgta ttaacgatta tgcctctaaa
    25441 cgcttttttt atgaaaaaat ttataagtga aaacgtaggt atgatattat ctagaggtaa
    25501 tttttttggg tatttaaata gttgctagga ggatagcttt tggattttta cagtaatttt
    25561 atcttaataa tagcaatatt attacttcta aatatttggt tttttgataa gagtagaaat
    25621 gcagggatag gttttagaac caaacgaagt acgtcttctg aaaaaaagtg ggtatattca
    25681 cagactattt tttacggagg ggttatttca atcagtttac tttcttcaac actttattct
    25741 tttaatgtta tagacgtttc catgtcaaat tttatatcta ttattggaat ccttatttct
    25801 gcaattatta cacaactact tcttgtgttt gaagagaagt caaaaaataa ttaaagcaat
    25861 tatgcctttt agtacaggaa ttattacttc ttacaaatgt atagcgaatg tgaagcctgc
    25921 tgttttaatt gttctagaaa aacttttgag tgaacaatta ttttatgtta ggatgaatta
    25981 atttctctag agtttataat
//
LOCUS       DACGLW010000038        25365 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.038, whole genome shotgun sequence.
ACCESSION   DACGLW010000038 DACGLW010000000
VERSION     DACGLW010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25365
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.038"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            complement(3..2060)
                     /locus_tag="IXT53_001544"
     CDS             complement(3..2060)
                     /locus_tag="IXT53_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HAQ4905375.1"
                     /translation="MKLFNPKTVLAIGTFAASLSLAGSVDAASTNVMYRLYNPNNNEH
                     FYTSSKKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNKLDSAYKGLKKKPVPTVNKAELQALYNRVKSTAKGDFTEESWNNFQTAL
                     SNAKKVLDDSKVSQTQVNNAKNSLDAAFKGLQHKPETPKEYTVTINHIDNQTGQVIAT
                     DQAKVTSGSTYTAKAKAFKYSEGAKDNFSYKVIGNDTQSKTITGNTTITFNYNQVHQI
                     NLLCNNNYKNEGRVNILKEQIVNVTHGDSVTLTAPSIQGYVLDDRVEPTNSVTLNNVT
                     ESQNYIFNYTRKFNVTVNHVNADTNAVLSSESKTVYEGDSFATPWKNMTDQNYFLCRN
                     DDPTVTVDKNGQRSINNVDQNKTITFKYKKISLDELRNFMRQKELAWLNDYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENISQTGLYID
                     DLLSDDGAIDAMVGWKNSPAHNANLLYNNQSILGLGHNFVVDSAGRIGVDNVFISANP
                     IFK"
     gene            complement(2174..2386)
                     /locus_tag="IXT53_001545"
     CDS             complement(2174..2386)
                     /locus_tag="IXT53_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905376.1"
                     /translation="MYADKKKIEWLLFDSGKSVRSISIESGVPLTTVSDLANKRSSID
                     NMRFINAMKLNKLAEEIVDINTSTNK"
     gene            2573..3040
                     /locus_tag="IXT53_001546"
     CDS             2573..3040
                     /locus_tag="IXT53_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905377.1"
                     /translation="MKKKQLWFVSMFAIFFIILSGCSSNKKSEAKEDNSLLQPGTVWK
                     EEVGGLIHNLKIIDDTTWEYSESVWHPDPVQITVKRQKDYEGLERYKIVDSAGIREFI
                     NKPDSLFIVVPYEKDGVKKIIFLGSSKDEKRTKEKLIHDGSELNKYKLQKTSE"
     gene            3037..3279
                     /locus_tag="IXT53_001547"
     CDS             3037..3279
                     /locus_tag="IXT53_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905378.1"
                     /translation="MIASLFIYPEGGITVAKVKTPFEKAASKILERRGVSEIQWKKEV
                     LEKAQFDLLRGKDKDLEEYIYQREREKLVFDEIIKH"
     gene            3297..3599
                     /locus_tag="IXT53_001548"
     CDS             3297..3599
                     /locus_tag="IXT53_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905379.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAVAA
                     VASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVILAAASIVVSMFSMFGQTPQGL"
     gene            3671..5725
                     /locus_tag="IXT53_001549"
     CDS             3671..5725
                     /locus_tag="IXT53_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905380.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLIDLIR
                     LDVFKDTDETMSVEESYRNEKLLQVLEEELESDQRKVNQVSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETDEVEVDPVEA
                     NEESTWTEGDFQEYIEPLISEEVEKNETEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKVKEELNKEEQRVIEEEVQKVKERYEKKRDNAYSLCVE
                     KINNFTKTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKHEQIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLEIRKREQD
                     LAEQKMAIEKQRLEQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPVAAVQTPVNNQ
                     ANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTASIKEEAQE
                     QARKEYEQQLEELLNRTNQSTTESSMENSKESDSSKAKEGSSDSTVQSNTSESSESNE
                     VK"
     gene            5725..8337
                     /locus_tag="IXT53_001550"
     CDS             5725..8337
                     /locus_tag="IXT53_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HAQ4905381.1"
                     /translation="MRVRETTKRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     KKIFYSGLVYLPLEYLVTRVPEVFNRLEIVKGSLQGINLFQLQEMPQVFHFDSAGFWN
                     PFTPTTSLGTRLAISTVCVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPENEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSIGFPKYLDLKLHESLLNQRIVVEFYHKGK
                     KFHEERVKVGYRGFCEINFDCHLEQGDHVCLKYNHQGKQYVAWYEFSQKVLKDEEGKI
                     VYRKKRGETHLPELEKEAVLTLDEKKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSFENETAKKLLIQFYEEQATDRLQEYVEKLIKNEEISEMI
                     RKRLLLEAEKAVKE"
     gene            8334..10712
                     /locus_tag="IXT53_001551"
     CDS             8334..10712
                     /locus_tag="IXT53_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905382.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDADPKTVTKVLQDYGNYLQYQDV
                     FSWICNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNELFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKNFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PDQAKKMSESSDISTVQKDRLKALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYRRY
                     ISSPGIILAGESSLSVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSAIE
                     DISQSFLLLAFTILELRIYTLMLSGIGDLHAVGKINGFLYVVALIVLTIALFKGSQSV
                     TKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGFGH
                     RKDITHDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGTVE
                     DLTEKAKDTLEEKLDGTRAKEFAEAVKNPSEALEKQKEKASAVKEGLKDAYGEGQVNA
                     AVKANTTSEKENQEGAAIPSAKDHQDELHSNENSIGEKDSEEGQHGQLPKTDPEQYRY
                     KSNINSENEKRISDLPKRNQEVQTDEDSIDLPNDNGIEVGTEKSTKIGLPKTQNLPMK
                     DSRLNQFKENDQSFVHQSPAGTVQMKSSRLPENGNLTTVSYNLAESGETENASVQNIV
                     TKEAAPKPINKVHNGYRPANVAFTAPTKINVTRETKNRNNELKEQLLKDIKP"
     gene            10773..11315
                     /locus_tag="IXT53_001552"
     CDS             10773..11315
                     /locus_tag="IXT53_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905383.1"
                     /translation="MEDILIATFITALGAFLTIFVVTIKGFKDTDSLQKDHQSILDGQ
                     KTHTESIIVHLSEGFTYLKDAMPTFTVKLTEIFHSVEQISSELKEQRQQKDRQLGSLN
                     FRQETIQESLENVNRLNDEMQRVHLENEELRAQNYDLIQKKRNLEQKIVELKEENRQL
                     NYQLRRGKNRSRDLGNELEL"
     gene            11393..11725
                     /locus_tag="IXT53_001553"
     CDS             11393..11725
                     /locus_tag="IXT53_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905384.1"
                     /translation="MDKYIHMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKIVNNLV
                     HLPLFFALLNYGLWLAIALVIVWRPYNNPGQRQIFAIIESIFFTDKNHYHALDPNRNY
                     HGTYKEDE"
     gene            11726..12319
                     /locus_tag="IXT53_001554"
     CDS             11726..12319
                     /locus_tag="IXT53_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905385.1"
                     /translation="MRVKGQNSVDKKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLRKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            12341..14035
                     /locus_tag="IXT53_001555"
     CDS             12341..14035
                     /locus_tag="IXT53_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HAQ4905386.1"
                     /translation="MLDVIKTAFSTQSNQKVIDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTDTQVTGKDPEAYPTFKEFLAFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNGATCSAVKSCGLQVV"
     gene            14723..16600
                     /gene="ltrA"
                     /locus_tag="IXT53_001556"
     CDS             14723..16600
                     /gene="ltrA"
                     /locus_tag="IXT53_001556"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ4905387.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
     gene            16731..17108
                     /locus_tag="IXT53_001557"
                     /pseudo
     CDS             16731..17108
                     /locus_tag="IXT53_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
     gene            17108..18313
                     /locus_tag="IXT53_001558"
     CDS             17108..18313
                     /locus_tag="IXT53_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAQ4905388.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWLFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDP
                     LPFLNSSKK"
     gene            18329..18973
                     /locus_tag="IXT53_001559"
     CDS             18329..18973
                     /locus_tag="IXT53_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905389.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQAANTYKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPTDDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVIDFSYFTR"
     gene            18984..19790
                     /locus_tag="IXT53_001560"
     CDS             18984..19790
                     /locus_tag="IXT53_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905390.1"
                     /translation="MSKKKGERQRFKKRKSYLRWLGIFMILGFLILASPIYTNQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIRAKVIKVSDDYFAVKLTHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQLKDQEEIIQKLQEKIDRMTKEKI"
     gene            19812..20042
                     /locus_tag="IXT53_001561"
     CDS             19812..20042
                     /locus_tag="IXT53_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905391.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
     gene            20588..20863
                     /locus_tag="IXT53_001562"
     CDS             20588..20863
                     /locus_tag="IXT53_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905392.1"
                     /translation="MDLLIRNIEEKYINKIDKRCEELTVKTGKKWSRNQYLKVLIEND
                     FDHALLNYKKDQFDRLLEKFVDIQTYNTKTLEEYIATNNQLIGLLIE"
     gene            20905..21327
                     /locus_tag="IXT53_001563"
     CDS             20905..21327
                     /locus_tag="IXT53_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905393.1"
                     /translation="MAKATRKHVYLYDMHIEVLDEIISTHEDISNYSEAVRHLCMMYD
                     PKIEEKINPIQIKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAEERVE
                     GKIKSNQTKKYSSKLKKPVNESVHTRVDPFDPTKILKR"
     gene            21346..22938
                     /locus_tag="IXT53_001564"
     CDS             21346..22938
                     /locus_tag="IXT53_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905394.1"
                     /translation="MNKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEMERRTPEE
                     EQELQRIKNALEDFYIPEGETFSESKTDKEFSETSNEAQSLMEHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKELTQREQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVISHDNSYLEKMGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEIENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPEKRSEWNYGDENRTKLSPFTRGKLDELTKYVLTKEPGYQEYIQ
                     ISKALKEEAKSLYGESKRETKNAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVN
                     QMQSIYNERSFRTLKQLPTNEYKYSYQEFVKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            22950..23285
                     /locus_tag="IXT53_001565"
     CDS             22950..23285
                     /locus_tag="IXT53_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905395.1"
                     /translation="MKQYLDQWKVIEGSLKEERIEQLPDCLEKEHLFQISEMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEINEEGINNFPCESI
                     EESISLTEC"
     gene            23432..23626
                     /locus_tag="IXT53_001566"
     CDS             23432..23626
                     /locus_tag="IXT53_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905396.1"
                     /translation="MTQTTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQNGNI
                     LTLNLTTKMFVDAVENYPFV"
     gene            23658..>25365
                     /locus_tag="IXT53_001567"
     CDS             23658..>25365
                     /locus_tag="IXT53_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905397.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNNQFQKLFDHQKLTNGQ
                     PMNDKRNGRSWFNQQEKIENKIQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKEKDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNHPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSSEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNT"
BASE COUNT         9348 a         3961 c         5156 g         6900 t
ORIGIN      
        1 ttttatttga agattggatt cgcactaata aatacattat ctacgccaat tctaccagca
       61 gagtcaacta caaaattatg acctaatccg agaatacttt ggttattata aagtaaattt
      121 gcattatggg cagggctatt tttccatcct accatggcat cgatagcacc atcgtctgat
      181 aaaaggtcat caatatacag cccagtttga cttatgtttt ctcccttagc accgtagcag
      241 cctaagcttt ccaataaatc ttggaaagta ccaccaccag ggcgatagtg actgaagctg
      301 acttgtaatt ctttagctcg aatatctgca gcctgttgaa caatatcatt gaattgcatt
      361 ggggcaacac cattttgttg gcgataatca tttaaccaag caagttcttt ttgacgcata
      421 aagttacgta attcgtctaa tgaaatcttt ttatatttga atgtgattgt tttattttga
      481 tcgacgttat taatggatcg ttggccgttt ttatccacgg taacggtagg atcgtcattt
      541 ctgcacaaga aataattttg atcagtcatg tttttccaag gagtagcaaa gctatcccct
      601 tcgtatactg tttttgattc actagagaga acagcgttgg tatcagcatt tacatgatta
      661 acagtcacat taaatttacg agtgtagttg aaaatataat tttgactttc agtcacattg
      721 tttaaggtta ctgaatttgt tggttctacc cgatcgtcta aaacatatcc ttgaatagaa
      781 ggagcagtta aagtgacact gtcaccgtga gtgacattga ctatctgttc tttcaaaata
      841 ttaactcgtc cttcgttttt gtagttgtta ttgcacaaca aattgatttg atgcacttga
      901 ttataattga atgtgattgt tgtattacct gtgattgttt tgctttgagt atcattacca
      961 atcactttgt agctaaaatt atctttagcg ccttcgctat atttaaatgc ttttgcctta
     1021 gcagtgtaag tagaaccaga agttactttt gcttgatctg tagcaatcac ttgacctgtt
     1081 tgattgtcaa tatggttaat agtaaccgta tattcttttg gtgtttcagg tttgtgttgc
     1141 aaccctttaa atgcagcatc aagactattt ttagcattgt ttacttgtgt ttgagaaacc
     1201 ttagaatcgt ctagtacttt tttagcattg gataaagcag tctgaaaatt attccaagat
     1261 tcttctgtaa aatctccttt agcagttgat tttactcggt tgtataaagc ttgtaattca
     1321 gctttattca cagtaggaac tggttttttc tttaatcctt tgtaagcaga atctaattta
     1381 tttttggcac tatccacttg cttttgagtc gcttttgagt catttaacac tttcttagca
     1441 ttgtttaaag cggtttggaa tgcgttccag gtattgtcgg tatagtcacc ttttttcgtt
     1501 cccttaacgc tcgtataaag agcttctaga gccgatttat tcactttggg tttagaggtg
     1561 tcagcgccat accaaccgat accttcgtat ttccagccat agtctttggt taaccattgt
     1621 acttctttcc aacttgcagt gtagtgatgt gagccagatt ttacaccagg aaggaaaagg
     1681 cggtagatcg gtttattatt tttttcggca gaacgccagg caggcccttc ataagtccag
     1741 ccgtaatttt tagtcaacca tttcacttca tcccaactag ctgtgtaatg gtgatcacga
     1801 agacctttat tgtacaaacg atagacaaga tggccaccgc tagtcggtgc ttcccaggcg
     1861 gggccttcat aatttcccca ttttatcttt actagatggt cacgttcttt tttgctagag
     1921 gtgtaaaaat gttcattatt atttggattg taaaggcgat acatgacgtt agttgaagca
     1981 gcgtcaacag atccagcaag actaagtgag gcagcaaatg ttccaatagc taaaacagtt
     2041 tttggattaa ataatttcat tttttcaact cctaaattta attaaaaatg tttacatgat
     2101 tattatatat cggctaaccg atatagtcaa taaaaaataa gaaaagcctt atataatttt
     2161 ggcttttctt attttatttg ttagttgaag tattaatatc aacgatttct tcagccaatt
     2221 tatttaattt catagcattt atgaatctca tgttgtctat actagagcgt ttgttggcaa
     2281 ggtcacttac tgtagttaag ggtacgccag attctatgga tatagaacga actgatttac
     2341 cactatcaaa cagcaaccat tcgattttct ttttatcagc gtacatttca atgctccttc
     2401 ttgttttttc tttattatag ctcttttttg taatgaatca caatatcaag agaaaaattt
     2461 tctatagaaa ggtggatttt tatgagtatt cgttctccgt gtatccaaat gaggtttgtt
     2521 gctactttcg ttatttgata tactaaaaat aaacaacgaa agaaggaata aaatgaagaa
     2581 aaagcaacta tggtttgtct ctatgtttgc aatatttttt attattttaa gtggatgttc
     2641 aagtaataag aaatcggaag ctaaagaaga taatagttta ttgcaaccag gaactgtttg
     2701 gaaagaagaa gtaggtggtc ttattcataa tttaaaaatt atagatgata ctacctggga
     2761 atattctgaa agtgtatggc atccagatcc tgtacaaata acagttaaga ggcaaaaaga
     2821 ctatgaaggt ttagaaagat acaaaatagt agattctgca ggcattaggg aatttataaa
     2881 caaacctgat tcattattta ttgttgttcc ctatgagaag gatggcgtaa aaaaaataat
     2941 ttttcttgga tctagcaagg atgagaagcg aacgaaagag aaattaattc atgatggatc
     3001 agagttaaat aaatacaaat tacaaaagac tagtgagtga ttgctagtct ttttatttat
     3061 cctgaaggag gaatcacagt ggcaaaagtt aaaacaccat tcgaaaaagc cgcgtctaaa
     3121 atcttagaac gccgtggtgt ttctgaaata cagtggaaaa aagaagttct tgaaaaagca
     3181 caatttgacc tattacgagg aaaagataag gacttagaag aatatatcta tcaacgtgaa
     3241 cgtgaaaaac ttgtattcga tgaaattatt aaacattaaa aggagcgaat aatcttatgt
     3301 ttgaatcaat tacaatgaat tttttaactc aaccgaaagt agtagctgca gttgatattt
     3361 ttagagcatt acaaactgcc gcaactagtt tgaaaaataa tctgatgatt tttgctgctg
     3421 ttgcggcagt ggcttccatt gctcttggag gtattttctt cttctttggg cgaagaggag
     3481 ctgaaaacgg gaagacacac gtaggttatg tattgttggg ggtaggagtt attttagctg
     3541 ccgcttctat tgtagtttca atgttctcaa tgtttggaca aaccccacaa ggactataaa
     3601 aaaaacaagt aggaggagta aaaagtacgt ggctactttt tactcctttt tttatgaaag
     3661 gagaatattc atgtttttta cgatttcagg aaatgcaaaa ttggataaag agttaataag
     3721 aaaagcaacg aagaaagtgg gaaagcgtac ttccttttta ccgaaaacaa ttaaatattc
     3781 tgtcattgaa acggaagaac agctacatgt gttaggacaa gtgttagaac aagagaatca
     3841 atcttctgtt aaattgactc tcacgttaca tgataaagaa ggaaaaatag aaaaggctgg
     3901 cacagaatac gtttttggcc cactggatta tcaaatttca gaaggttttt atcctacatt
     3961 gatagattta atccgccttg atgtgtttaa agatacggat gaaactatga gtgtggaaga
     4021 gtcttatcga aacgaaaaac ttttacaagt tttagaagaa gagttagaga gcgaccaaag
     4081 aaaagtgaat caagtgagtg aagtcgaagt aaatgaagaa gtaccatggg aacgaccaat
     4141 ctttccaaag aaggagtcaa aagaaaaaga gacagtctat gttgacgaac aagctgatat
     4201 tccgactcag aaatcgatat ttgaggaaga gaacgatgtg atgggtcaat tggaggtaga
     4261 tccctttgaa gaaacaatgg aggatgaaga gacggacgaa gtagaagttg atccagttga
     4321 agctaatgaa gagtcaacat ggactgaagg ggattttcag gaatatattg aaccacttat
     4381 cagtgaagaa gtagaaaaaa acgaaacaga gatacaaaag gcaactgtat ttgattatat
     4441 tttggatcaa tatccgccag agcatgaatg gatgaaagaa aaaaacaaat tatttttaaa
     4501 tgagctttat tcgaaatatc aattacccca aacctatcaa gcatttttag agagtaaaga
     4561 agaattaatc tcacagggta aaggcacact tttagaacag ttagagtctg tccaatcaac
     4621 aaattggcat gagcttgcac aagaagattt attagccacc tttgaagaac gtcaacaaga
     4681 aacagatcaa gaaattaatg agtatgcgac ggaacagtcg tctaattggg agaaagtaaa
     4741 agaggaacta aataaagaag aacagcgagt gattgaagaa gaagtacaaa aagtcaaaga
     4801 gcggtacgaa aagaaacgcg acaatgctta ttccttgtgt gtagaaaaga tcaacaattt
     4861 tactaagacg aatcaagaag cgttatcgaa agaaaaagaa aacttgttaa aagaacgtat
     4921 taaagaacga aaagaagagt tctaccgcgg tttacgtaca gataagctaa agattagcaa
     4981 agaattaaat gaacaactga ataagctata tcaaacaacg actgaagttc ttatggaaaa
     5041 acacgaacag atccagcaag aattacaagt acaaattcca aaatggaaaa aagagtatga
     5101 aattcaacaa caagaattga aagaacaaga agaaaaacaa cggcaaaaag aaaaagagcg
     5161 tgaacaatta gaaataagaa aacgggaaca ggatcttgcg gaacaaaaaa tggcgataga
     5221 aaaacaacgg ttagagcaag aaaaggaaaa agaagcagct gctcgtaagg aacgtcaaga
     5281 acaaatggat acattgcgtc aagcacagtt gaattatcta gcacaacctc aaccagtagc
     5341 tgctgttcaa acgccagtga acaaccaagc aaatttaact ccttcacccc agcgttcctt
     5401 taacggatgg atggtgggat gtattgcaag tttaagcttg cttctaggtg gaggaacagt
     5461 atttgctttt aatcatatca atcaatcttc acaggctgat tctgtagcca caacggcgtc
     5521 aatcaaggaa gaagcccagg aacaagcaag aaaagagtat gaacaacaat tagaagaatt
     5581 attgaatcgt accaatcagt caacaacaga gtcaagcatg gaaaatagca aagagagtga
     5641 ttctagtaag gctaaagaag gatcaagcga ttcaactgta caatcaaaca cttccgaaag
     5701 ttcagaatca aatgaggtga aataatgcgt gttagagaaa caacgaagcg aaaagacaaa
     5761 cgattcaatt tagaaaagaa taggtatctt tctcatttct atcgctatac tccctcagga
     5821 gaagagctac cagaaaaaca tgacaaacgc atttcgaaaa agatttttta tagtggactt
     5881 gtatacctcc ctttagagta tttagtaaca agagtaccag aagttttcaa ccgattggaa
     5941 attgttaagg gaagcttaca aggaataaat ctgtttcaat tacaggaaat gccacaggtc
     6001 tttcattttg attcagctgg tttttggaat ccatttactc ctacaactag tttgggtaca
     6061 cgtttagcaa tttcgacagt ttgtgtggtt ccagctgtac ttatgggatt gaaaatggac
     6121 atgtggtttc gtcctctagc agatggacaa aaaggggata atcgattaat gaccttaaaa
     6181 gaaatccaac agatttatcc gcaaatacct gaaacaaaaa ctacttttcc aggttatgga
     6241 ggtattccaa tcacacatta caaaaaatat tggtacattc aaacagatac tgttaatacg
     6301 tgtattgttg gtacctctcg atcagggaaa ggtcagattg aagtgttagc caccattgac
     6361 aatttatctc gtgcagaaaa acaaagttca atggtcgtta atgatccaaa aacagaatta
     6421 tatgttggta gtaaagatga actagaagct agagggtatg atgtatatgc ctttaatatc
     6481 accgatccac tgcaatcaat gagtgataat ccattggcac tcattgtgaa atactggaag
     6541 cgaggagaca tagacaccgc aacacagtta accaatactt ttacgaatac gatttattat
     6601 gatgcaaatg cttcagacaa taaatacttt aacgaaaatg cgcagaaagc tgtcaatgcg
     6661 cttatttttg cgttattgga acaagcggat caaacagggg attactccaa attaacacca
     6721 aataatctcg ttgaactact ttcagaaatt ggcggtttta attattatcc agatcctgag
     6781 aatgaattca agcagctcaa tgccttagat gagttcatga atcagttacc ccctggaaat
     6841 atagcaaaaa aacaatatgg ggcaacaaaa attggatctg ataaagcgaa agggaatatc
     6901 ttgtctacgg caattacagg attgaatcca tttaccctaa cgaaaattgc gaagatgact
     6961 tctcaatcga ccattgatta caagtccatt ggttttccga agtatttgga tctcaaatta
     7021 catgaaagcc tattaaatca acgcattgtc gtggaatttt atcataaggg aaagaaattt
     7081 cacgaggaac gagtcaaagt tggctatcgt ggtttttgtg aaatcaactt tgattgtcat
     7141 ttagagcagg gagatcatgt ttgcttaaaa tacaatcatc aaggaaaaca atatgttgct
     7201 tggtatgagt tttctcaaaa agtattgaaa gacgaagagg gaaaaatcgt ttatcgaaaa
     7261 aaacgaggag aaactcattt gccagaacta gaaaaagaag ccgttctaac attagatgag
     7321 aagaaaagta acctattagt agagtctatt cggatgcact ataccgataa accaatggct
     7381 atttttatgt taacgcctga ttatgatcca tctaaccata tcattgttag catctttctt
     7441 agtcagttgt ataaagaatt atctacgcaa tgtgttaaga ctaaaggaga taaatgtcac
     7501 cgtaggattc attttatttt agatgaattt ggaaatatgc caccgatgga caacatggaa
     7561 ggaatcatga cggttacggc aggtcgtaat atgttatggg atctgtttat tcaatcttat
     7621 cagcaacttt atgcgaagta tggagaacaa aacgcagcga ttatcaaaga aaattgtcag
     7681 acacatattt atattatgtc gacagatgat aataccatcg aagaattttc taaaaaagtg
     7741 gggaataaga cggttgaaca agagaacagt accatcaatt cattaggtat gaatactcat
     7801 atcaatcgca atgttgattc agatcgtatc ttaactcctg aaagaatttc tactttttta
     7861 gaaggtgaaa cgattgtact cgctccttta aaacgacaag atttgaaagg tcataaaata
     7921 cggccgtatc ctatttttaa tcatggggaa acgaatcttc cttatgctta tcaatatatt
     7981 gaagatttca atccatcgtg ggatatcaat gatatagaca tacattgtac acatgcggac
     8041 ttggatctta aggctttaca aattgattat gattatttcc taccagatga atatgctaga
     8101 aatctctatc atggactgca tgatcaatta gaagaaaaaa acgaagaaaa tatgttttat
     8161 aaaatcgttc aatcttttga gaatgagacg gctaaaaaat tgttaattca attttacgaa
     8221 gaacaagcaa cggatcgtct tcaagaatat gttgaaaagt tgatcaaaaa tgaagaaatt
     8281 tctgaaatga ttaggaaacg gctgttattg gaagctgaaa aggcggtgaa agaatgatca
     8341 ttggaggaat tttctctggc atagccgact ggtggtcagg tttattcgga gaaggggatg
     8401 cagatcccaa aaccgtaacg aaagtcttac aagattatgg taattatctg cagtatcaag
     8461 atgtattcag ttggatttgt aatacttttt tatggggatt gatcaaaggg ttatataatc
     8521 tcaatacttt attagaaaaa acaatttatc aaagctttga tttaaaagac ttattgaacg
     8581 ccgctggtgt taatgaacta tttcaaagtt tgattagtaa ggtattggct cttttttgtg
     8641 tcgtcacatt aatttattta ggcttgaaat tttttatgag taaacaccca ccgaaagtca
     8701 aaaatatctt ggttaattta tttatggcca tgatcttgat ttttggtggt agttctttaa
     8761 tcgatgaagg gttaaatatc agtaaaaact tttatggaga cgtcactgca gcaaataaag
     8821 cagataaaac gggttcacct gcttttcaaa ttattcaaaa taatgtgatt gatgtcagta
     8881 cattactagc gactgacccg aataaagtag aatccttacc aacagataaa agaaatgctt
     8941 taacagcgaa aaatttcaaa acagcgaata tcaatgggat tattacacct gatcaagcta
     9001 agaaaatgtc tgaatcaagt gatattagta cagtacaaaa agatcggtta aaggcattac
     9061 agtatcgact agagttagac gataatggga aagaaatccc tgttcaaatt agtgatgaag
     9121 gtttagcaaa atatgtgtat acgtcaggct atcgtcgata cattagtagt ccagggatta
     9181 ttttggctgg ggaatcttct ttgtcggtag cctatttatt tattttgttt acaatcgtta
     9241 cttgcattat tgagctagta tttaaaaaat tctatttagt aattgccgcg tcaacggact
     9301 ttgagacagg acaacggatg aaaagtgcga tagaagatat ttctcaatcc ttcttgttat
     9361 tggcgtttac gattttagaa ttacgaatct atacattaat gctttcaggt atcggtgatt
     9421 tgcatgcggt aggaaagatc aatggattct tgtatgttgt tgctttaatt gtgctaacta
     9481 ttgcgctatt caaaggctct caatcggtta cgaagatttt cggtgttgat acgagtctga
     9541 aaaacggcag caattctctt cttagcatgt ttgccttagg aaatattgcg aaaaatgtgg
     9601 gtagcggagc taaaaacctt gcaagtgctg gtaagagtgg tttaagccat ttaaatgata
     9661 ttcgtaagaa tggatttggc catcgaaaag atattaccca tgataacggg gaagaacaat
     9721 cagacgataa agctacaact gtacctaata gtaagaagac agtttccgaa atgttcggga
     9781 aagcgaaaaa tggtgtttct aaagccgcag aaggtcttgg ttacgtcaat gaacgtgggt
     9841 taaaaggaac agttgaagat cttacagaaa aagccaaaga caccttagaa gagaaattag
     9901 acggcacgag agctaaagaa tttgctgaag ctgtgaagaa tccatccgaa gcactagaaa
     9961 aacaaaaaga aaaagcttca gcagtcaaag aaggactaaa agatgcgtat ggtgaaggac
    10021 aagtcaatgc agcagtgaag gcaaatacaa caagtgaaaa agaaaatcaa gaaggtgcag
    10081 cgattccttc agccaaagat caccaagatg agttacattc taacgaaaat tcgatcggag
    10141 aaaaagattc agaagaagga caacatggtc agttaccaaa aaccgatcca gaacaatatc
    10201 gttataaatc taacattaat tctgaaaacg aaaaacgcat ttcagattta ccaaaacgaa
    10261 atcaagaagt acaaacggat gaggattcca tcgatctgcc aaatgataat ggcatcgaag
    10321 ttggaactga aaaatcaacg aaaattggtt tgcctaagac acaaaattta ccaatgaaag
    10381 atagtcgttt gaatcaattc aaagagaatg atcaaagctt tgttcatcaa tcacctgcag
    10441 gtactgttca aatgaaatca agtcgtttgc ctgaaaatgg aaatctgaca actgtttctt
    10501 acaatttagc tgaaagtggt gaaacagaga atgcgtccgt tcaaaatatt gtgacaaaag
    10561 aagctgcacc taagccaatc aataaagttc ataatggata cagaccagca aacgtagcat
    10621 ttactgcacc gacaaaaata aatgtgacaa gagaaactaa aaacaggaac aatgaattga
    10681 aagaacaact attgaaggat atcaaaccat aaaaggccta tttaagggcc tttttcttta
    10741 taaaacaagt ttaaaatgga aaggagaaaa ttgtggagga tattttaata gctacgttta
    10801 taactgcact gggagcattt ttaacgatat ttgtggtgac tattaaagga tttaaagata
    10861 cggatagttt acaaaaagat catcagtcta ttttagatgg tcaaaaaaca cataccgaaa
    10921 gcattattgt acacctttca gaaggattca cttatttgaa agatgctatg cccacattca
    10981 cagtaaaatt aacggaaata tttcattctg ttgagcaaat atcgtcagaa ttgaaagagc
    11041 aaagacaaca aaaagatcgt caacttggct cacttaattt taggcaagaa acgatacaag
    11101 aatcattgga gaatgtaaat cgattaaacg atgaaatgca acgagttcat ttggaaaatg
    11161 aagaattacg cgcacaaaat tatgatttaa ttcaaaagaa aagaaatctg gaacaaaaaa
    11221 ttgttgaatt aaaagaagaa aatcgtcaat tgaattatca attacgacga ggaaaaaatc
    11281 gttctcgtga tttaggaaat gaattagagt tgtaatattt agaaaaacgc tgtgagaaat
    11341 cacagtgttt tttatttcta aaaatacaaa atataagggg tatggttata ttatggataa
    11401 atacattcat atgaaaaatc ctccaagatt tagtggggaa tggaagtttt tcgccttttt
    11461 ctttttagat ctattaacgc ttggtggctg ctttttttgg gcgaaaatag tcaataatct
    11521 tgtccactta ccactctttt ttgcactctt gaattatgga ttatggcttg cgatagcgtt
    11581 agtgattgtt tggcgaccct ataataaccc tggtcaacgc caaatttttg cgattatcga
    11641 atctattttc tttacggata agaaccacta tcatgcgtta gatcctaacc gaaattatca
    11701 tggtacgtat aaggaggatg aataaatgcg agtcaaagga caaaattcag ttgataagaa
    11761 atccattttg cagtttggaa aaccagaaga gcgctctatc ttggacttat ttccatataa
    11821 atcctgtaca gaagagggag tcttaattac tagagaagat cattttcagc gttattatcg
    11881 tgtcgtttcc actgacgtgg agggattaaa tgaacaagag aaactagaac gaatgaatca
    11941 gttgaccacc gtcatgcgta cgtatgtgcc aacaattaag ttagttagtt tgaccacaga
    12001 gacggatcta acggaacaaa tcgtgcaaaa gcggcagtta ttagataaaa atcgtatgga
    12061 acagtcacta ggaagaaatc tacagcgatt aagaaaatat gaaaagaaat tagtagagga
    12121 gatccaggaa ttaaaacggg cagaacaaga acgtcctgac ttaagcttct ttttcgttat
    12181 tgaaggaaag acaatcaagg atttacaaac tagaaatcgt cagttaatgc gatctagtgg
    12241 cattttaggg ttaaaaccac tctacaaaaa agaattgatt aagattcttt atcgaatgaa
    12301 caacatgaat gacgaataaa aaagatggag gaaatgattc atgctagatg tgataaaaac
    12361 agctttttca acgcaaagta atcaaaaggt gattgatcca ttaaaacaac ttggtaatcg
    12421 tgaaatagag attttaaaat caaaaggtta tgatttagat ttcttaaaac aagtgatgcc
    12481 acaaggtggg ttgaattttg atgaagaata tgtgacctat ggaaatggtt attcacgatg
    12541 tgtgacagtt tatcattaca caaaaaatcc tactcttttt tggttggcca atctcatgaa
    12601 caatcctcat acagtggcca cactagatgt tagcacagat gaaaagcaaa aagtgattga
    12661 tcaaatcaac cgcgcattag atgaattaaa tgatcgatca gataatgaac gaaaagcgac
    12721 tgaccgaaat aaaagtgcgg aagaatacct ggaactagcg gaatatgccc gaaaattgac
    12781 ttctaatggg gaaatttcta aacaagtcaa agtgagagta catgtttacg gaaatacgat
    12841 ccaagaagtg gatgaacggg cgcaggaact gatcgaaaat ctaaaaggga tggattatcg
    12901 tgcggtgact tatctgcata aaaccaaacg agattggcaa tcgatgttta ccatgttcga
    12961 tgaacaagct gaatggtttg gaaatatcaa agggcaatca cttccatcgt taaacattgg
    13021 cggaggattc ccgttccacc accaaacgct aaaagatcca ggaggcacac aatatgggga
    13081 aaccatgaca ggcggcccat ttgtctggaa tgccacgtat aaaaatagtg tacgtttatc
    13141 taccaatacc ttgcttctag gaatgatggg agctggtaaa tcgaccgttc ttaaaatgat
    13201 cgcggaagcc catttagctg caggagactt tatttggggc tttgaaaagg gaaaagactt
    13261 tattcctttc ttaaaggaat ataacggtat tatggtgcgt ttagacggat ctgacggaat
    13321 gatcaatcct ttagagatat ttgccacacg gacgtatgat gaagcatcca gtttatatga
    13381 cgatggatca gtcaaagatt tgaagatcaa tgaagcagcg tcctatcaaa cacacttaga
    13441 caaagtggtc tatcaggtac agttagtgag tcctcagcta aaaggaacga tgaaagcaga
    13501 atttaagacc tacttaaatc gtttttatga agagtatgga acagtaccac gcggctttac
    13561 ttcctcaaat tcgcgttcaa atactgatac gcaagtcaca gggaaagatc cagaagctta
    13621 tccgacgttc aaagaatttt tagccttttt aggacaactg gaactaccag gggcttccca
    13681 agagaaaaag aatcggaaag aagaaatgga gtctatcgtg gaatccttat gtgagactta
    13741 tggcatgatt tttgatggtc atagtacgat tcgacacctt gaccaacaac agttggtctt
    13801 ttttgacatt gactctatta caggcctaga tcgaggggta caacaatgtc agatgtacat
    13861 ggccatgacg ttgatttgga atcaggcatt gattcaagga cgaagacaac gacgcttaat
    13921 taaagaaaaa caaatggaag aacaagactt caaacgcttc ttggcgatga ttgatgagtg
    13981 ccataatggt gcgacctgtt ctgcagtgaa aagctgtggg ttgcaagtag tctaaaaact
    14041 attgtaataa ctcttataac gagaagtcaa atgagtgggt aacgccacaa agggcttcca
    14101 ctgatactcc gacatgcaac aagtagttta tgcagaccaa cgttgtgtga agctcggtga
    14161 agtcggctga aggataccgt aaggtaaaag aaatagtgaa taccacgaaa gttctctaga
    14221 ggtaaagggt gtatccgata agtcgggtgt ccaaaaaaac ttaatatggt gagtatgtac
    14281 aaacgtactg acgaactagc gaatgtactg tccgaaaggc atagctccta gaaatgggag
    14341 agacagtgag caaaaaatca ctcattgtgc taacttgtgg ttgagtaaaa gtcgttgtta
    14401 tgaaagacca tgctgcgtta caggcgtagg agaagttagg gggcaaaagc tagcatctaa
    14461 gttaaggcat gtggatagat ataagggaac aggggaagcc atcaacatgg agagtgcata
    14521 ctcgatgaag tggtagtaag aaatatgtga atgacaaata acttacttta gtgatggtga
    14581 aagtagagtc acgactgatg aaggttctgt aatggaactg gaggaacagg ctcgattcgt
    14641 ctgtaaaagt catgaaaccc gttcaaccat gggattctcg tatgactaaa aaggtcacga
    14701 gccaattgga gcgtgataaa ccatgacaaa agaaacaagt ccaaaagata ggaagttgcg
    14761 gcatgcagaa tattatggca tgacggaaat cttcgataag atatactcca aaagtttgaa
    14821 gggagataat ttcaagaatc taatgcagat tatcatttct gataacaaca ttgtcctagc
    14881 atatcgtaat atcaagagaa atcacggtag tgtgacaaga ggagaagatg gagtaacgat
    14941 taaagatatt gaaaatctca accgtaatga attcgtagaa attgttagga agcgattcgc
    15001 acattataac cctcggaaag taaggcgtgt agaaattcct aaatataatg gtaaattaag
    15061 accgttgggt attccatcaa tatgggatag aatcgcgcag caatgcatct tacaagtatt
    15121 agaaccaata tgtgaagcaa aattcaataa tcacagctat ggtttccgcc caaatagatc
    15181 agcagaacac gcaatagcag attgtatggc ccgaatgaat caaagtcaca tggaatatgt
    15241 ggtagatgta gatatcaaag gtttcttcga cgaagtcaat catactaaac tcatgagaca
    15301 aatctggtct atgggtatac gagacaaaca gcttctctta atcattagga aaatgttaag
    15361 agcacccgta gtacttccaa atgggaagtt gatataccca acaaaaggaa caccacaagg
    15421 cggaatactt agcccattac ttgcgaacat caatctcaac gaatttgact ggtggatagc
    15481 aaatcagtgg gaggaaaaag atgccaaaga gctaaaacat cattttagga aaaatggagc
    15541 ttgggataaa ggtaatatct atcacaagct cagaaagagt acaactctaa aagaaatata
    15601 cctagtgaga tacgcagatg attttaaaat tttctgtaga aattataatg aagcgaaaaa
    15661 agttttctat gcaactaaaa tgtggttaca ggagcgtcta aagttaccaa tatctgaaga
    15721 aaagtcaaaa atcacaaatc tttcaaaaga agaaagtgaa tttttaggct ttacgatgaa
    15781 attagtgacc aaaggtaaca aaaaggttgt taatacgcat ataggtacaa aagcacaaag
    15841 catgattaaa gtgaaactaa aaaaacaaat caaacgaatt caaaaaagtc ctaatagcaa
    15901 agataccttt atctatattg gagtctataa cagtatggtt attgggattc ataattactt
    15961 caaaattgcg actcattgta gtgtagcaat gcgaaaaatt gcaagagaac tcagaccaag
    16021 ttttgaaata cgactacgag aacaaggaat ctcaaaagaa ggacaatacg gaaagtacga
    16081 aggatacaag ccttacctac ggtcaggaca aatacgatat ataagagggt ttccaatatt
    16141 accagtaggg tatattcaac acagacatcc gatgtgtaaa aaaagaaata ttaataaata
    16201 caccgaagaa ggtagggaag ccattcataa gaagcaacaa accgtagaag aatggaagat
    16261 atcgtggttg tgtagcaatc cagtgcttag taagagagca acgattgaat ttgcggacaa
    16321 tcgaatttca aaattcatcg ctcaaaaggg aagatgtgca gtaacaggtg aggagcttat
    16381 cttatctgaa atgcattgtc atcatattat cccttatcat gaaagcaaaa gtgatagcta
    16441 tgaaaaccta gtgattgtta ctgaggaggt tcatcgggta atacacgcta cacaaagtga
    16501 aacgattgag gaacttctaa aatatctaaa attaaatcca aaacaaaaag aaaaactcaa
    16561 tgagttgaga ttaaaagttg gtaacgaaga aatatcttaa taaatatttg taggacggga
    16621 aaacagaatg tcttttatag acgagggaac gccgtatgcg gtgaaagtcg cacgtacggt
    16681 gtggactggg ggaaaagacg gagataatat caaactctta cctatcagta tcgattaatc
    16741 cagattttat tgaaacggta aattatgtca caaactttca acgagaaatg cgtaaagtag
    16801 aagcaatgac tgttttagct acacaatccc cacaagaaat ggtgccagaa aatatgtctt
    16861 ctgataactt ctcgaaactg aagaaagtct ttgaattttc ttctacgaag atctttatgc
    16921 gtatggatga atctatttta cgtcatatag aaggattagt gggtaaatca atgacgaact
    16981 cagaattaga atccattcct caacaaaatc aaggagacgc tgtgatcaat tttggctcaa
    17041 aagaaaccat tcgtgttcat tttagtccga acaaacgtca attaaaattg tttgatggag
    17101 gaaaataatg gaaaaccaaa atgaaagttt aatcaagcag tatgttaagc gtcgtgcaaa
    17161 gcggcgctta tttttgtggt tgtttggtac ttctgcaggt ttgattacta ttttgatcac
    17221 tgtgttcgtg accctatttc taatacttgc ggcgggctcg attgataatt cggattcaga
    17281 ttcaagcagt ggtggcgaag cttttacagg agaatattcg gaaggcttac cgatttataa
    17341 agaaatcaaa ggaagaggcc ctttttctga tgagattgcg caatatgccg taggtgcagc
    17401 agtgaaatat aaattattac caagtgtgat cctttcgcaa tacggttacg aatcagcgtt
    17461 tggtacatcg gcgtccgcaa gaaatgatct caactatttt gggattactt ggtttgatgg
    17521 ttgtctgttt cctaaaggaa ctgcacgagg aattggtggc attgaaggtg gctggtacat
    17581 gaagtttccc aactcaaaag ctgcattttc ctattacggc tttatggtag ccacacaatc
    17641 caattttaat gcttgtgtag gaaataaaag ccctggcgca agcttattga tcttaggacg
    17701 aggcggttat gccgctgcag gaattacgga agatagtccg tattatacag gttgtatgtc
    17761 aattatcact tctaataaat taaccgaata tgatgaattc gccatcaaac attggggtga
    17821 aggtggcaat aacaacggca cgattactgg tgaatggacg aatccattcc ctggcagcag
    17881 tttagataaa agttccttca gcggtggtca attatttgga acgaatccag gcggtgaatt
    17941 ccgtccgaac ggcttccatg atggattaga ttttggttca gttgatcatc caggaagtga
    18001 gattcatgcg gttcatggcg gaaaagtcgt gtacgttggc aatcctggta tttcaggttt
    18061 gggcgcgtgt gtgatcgtga ttaattacga tggcttgaat atggtgtatc aagaatttgc
    18121 aaatagtaca ggaaattctc gagtgaaagt aggcgatcaa gtgaaagttg gtcaagtaat
    18181 tgggatacga gatacagcac acttgcattt aggatttact cgaatggatt ggcgtcaagc
    18241 acaaggacat gcattcattg atgatggtac atggatcgat ccgttaccat ttttgaatag
    18301 cagtaaaaaa tagatcagga gaaaaaatat ggaaaataaa acgttaaaaa tcgtcttaac
    18361 agctgcaatt agcgttagtt taatcggcaa tattgcgttg ttttggcagc tgaaagagga
    18421 acaagcagca aatacctata aaattgaaga agtggctaaa aacgcgtctg agaagctcaa
    18481 aaaaaattcc tctgaggcac aggcgaagat cgacaagtta aatcaaacca tcaatgagaa
    18541 aaaccaagaa atcgatcagc tgaagcaaca agatggcaca aaagcaaaag aggaagtaac
    18601 tgaagcacaa agaaagacgg cagaagagtt cggtcaattg gccattgata aatcattaag
    18661 cagaaatgaa ttaacggaaa aactaaaaga tgtcgcaacc caagaagtga tcgacaaatt
    18721 aagtcctaca gacgatgatc accaacatga tgattacgga agttactcct ttacattagg
    18781 tgacgtgcaa acttacgctc aaacaagcag tgtaaaagag gaacaaaatt ttgtcgtatt
    18841 tgttgattat acaatcggca atccgcaatt caaagatgtc aaaccacaaa aaataaaagg
    18901 tggattaact gtcactgaaa aacaagtaga tggtcaatgg aaagtcatag actttagtta
    18961 tttcactcgt taggaggaag aaaatgagta agaaaaaagg agaaagacaa cgtttcaaaa
    19021 aacgaaaaag ttatcttaga tggctgggaa tctttatgat tctcggcttt ttgattcttg
    19081 ctagtccgat ttatacgaat cagaaggtag atataccaga tgaggtaagc acgaacgcct
    19141 ttttaaacac gaaagagctg acaatggttt caaaagaaaa aggagcgaac cagcaattag
    19201 tttttgaatt tgcgatcgat gatgaagatc aaagcttgtt aaaagaactg gctaatctta
    19261 attatcgcgt agaagtaaag acagctaaag gggattatca agcaatacga gccaaagtga
    19321 ttaaggtgtc tgatgattat tttgcagtga agctaacgca tgtaccagaa gaatatatcg
    19381 ccatgcgttt aaccattatt cctgaaaaaa ttgatccaaa agtggatatg caagaaccac
    19441 aagatctgat ttattacgtg cacgaagata aggttaaaga tcaagtgaaa gatgaagatt
    19501 atgaacaaca cgcgatcaat tacaaagtga aaggatacaa gaaagaacaa aaagcagctc
    19561 aaaaacaaat ggaatctttc caagcaacca ttgatttgaa tgaagaatta gtgaagaagc
    19621 taaaagatca attgccttat caagtcgcag atgatcagga aaatactgaa aacaagatta
    19681 atagctacca gcaagaaatt gaaacatcga aaacgcaact gaaagatcaa gaagagatca
    19741 ttcaaaaact tcaagaaaaa attgatcgta tgacaaaaga aaagatttag aaggacaggt
    19801 ggagtgaaaa aatgaagcga acaaacttgg ctgacaaaga tactttcgct gagcagaaag
    19861 tattgattaa agcctatctc aaagttgtaa aagctaaaga aatatgtcga gaagtgaatt
    19921 tatctaacac aacaatatct gagttgaaga atggtcgaaa gaatttagat actttaaaat
    19981 tcagatcatt tgaagctcta tatgtagctg ctaaaaaata ttttacagct gataacaaat
    20041 aaaaaacagg agtattttat gtgaaaaaag tatataaaaa atgcttatga atcagctata
    20101 tcaccttaac catttgttaa tgagatggga gaaaacatta accacgattc taacgcaaac
    20161 atcaagcaca taaaacattt aacactcctc ctttctggct gaagatttca accaaacagg
    20221 aagacagcgt atcaattata gcatacactt tttcttaaat tcatttttca tatgacaaaa
    20281 tatttccttc taatcgacaa agttagaagg attttttatt gtctaaatac acaaataacc
    20341 ttaattttta aagagcataa ggaaacgtga tatcactttc tcacgaaagg tgttaggtag
    20401 gctgtgccta ccacgtttct aagaaagata aatgatatca aaattttgga tttgtgtgta
    20461 gatttgtggg taggatgaaa gcgaatgtgg gcagtaaaat aagcgtttgt gtgtagcaaa
    20521 atcaaaaaat gtgtagggat atgtgtagct acacatatcc ctacacataa taaatttgga
    20581 ggaaaatatg gacttattaa ttagaaatat tgaagaaaag tatattaata aaattgacaa
    20641 acgttgtgaa gaacttacgg tcaagactgg caaaaaatgg agtcgaaatc aatacctaaa
    20701 agttttgatc gaaaatgatt ttgatcacgc tcttctgaat tataaaaaag accaatttga
    20761 tcggttgtta gaaaaatttg tggatattca aacttataat acgaaaactt tagaagaata
    20821 tattgcaaca aataatcaat taattggttt gttaattgaa taataaaaat agtattgtga
    20881 atttataaaa ggaggaaatc aattttggca aaagcaacaa gaaaacatgt atatctttac
    20941 gatatgcata tcgaagtact tgatgaaata atttctactc atgaagatat atctaattat
    21001 agtgaagcag tcagacattt gtgtatgatg tatgatccaa aaatcgaaga aaaaataaat
    21061 ccaattcaaa ttaaattgaa tgcaatggga aaagaaattt ctattttaac cgaaatggtt
    21121 tcagagtttg cggatcttca tttgaaagat acaggaatac taattggtac aaattcaact
    21181 gtttatcaag aagcagaaga acgtgtggaa ggaaaaatta aatccaatca aaccaaaaaa
    21241 tattcttcta agttaaagaa accagtgaat gaatctgttc ataccagagt agatccattt
    21301 gatccaacga agattctaaa aagataaaag tagaaggaga acgtgatgaa taaatcgcct
    21361 gcagttattc ttacttgtca atttaccttg cccaataaaa aatctttttc aacatacatt
    21421 gattatatga caagagaaaa agcactggaa gaaatggaac gacgtacgcc cgaagaagaa
    21481 caggaactgc aacgaatcaa gaatgcttta gaggattttt atattcctga aggagaaact
    21541 ttttcggaaa gtaagacgga taaagaattc agtgaaacgt cgaatgaagc tcaatcgttg
    21601 atggaacatg gaatcaattt tgacaagtta gaggaacaag attttaccaa atatctttct
    21661 tatatgaccc gtcactatgc gttagaacaa aagaaagaac tgacgcaaag ggaacaaaaa
    21721 gaataccaac ggttgaacca agcaatgaaa aaatatcaaa cagagccaca taaaaaagct
    21781 tcaggaaatc ttcctggagt tttttctatg gcttcagacg aagtgaaagg aagtgatctg
    21841 aaggagatca aacaaatctt ccaaagagga caacaaaaag gctcaatcat ttatcaagat
    21901 gttatctctc acgacaattc gtatcttgaa aaaatgggct tgtacgatcc caaaacagat
    21961 accttagatg aagaaggttt gaaagcagct ggaagaaaaa tgatggagac attattcgag
    22021 aaagaacaat tgaatgaaac aggttactgg atggcaacga ttcaccgtaa cactaagcat
    22081 atccatattc attttgcagt tgttgaaaaa gaaaatacgc gtaaaccgta tactgagatt
    22141 gaaaatggtg tgagttatat cgtaccgaag gggaagagaa gacaagcaac attagacgca
    22201 atgaaaacca attatgtaca tgagttagaa cgtttctctt atgaaaagga tcgaatgatc
    22261 ggtatggaga aacgaaattt attgacacga aaaagtgatt tgcgtaacac cttaacaaaa
    22321 gtagtaaaag aaccagcacg ttacgatcga cacgccatga acttattaaa tgaagtatat
    22381 aaaaccctac ctgaaaaacg ttcggaatgg aattatggag atgaaaatcg aaccaagctt
    22441 tcaccattta caaggggaaa acttgatgag ttaaccaaat atgttttaac aaaagaacct
    22501 ggatatcaag aatatataca gatttcaaaa gcattgaaag aagaagccaa aagcctttat
    22561 ggggaaagca aaagagaaac aaagaatgca gaaaaaaacg ccctctttga tttaaaaaag
    22621 cgcacaggaa atgcgatttt aacagaactg aagagacagg atagtcaaat aaaaccatta
    22681 atagaaagtg tcaatcagat gcaatctatt tacaatgaac gatcctttcg aacactcaaa
    22741 cagttgccaa caaatgaata caagtatagt tatcaagaat tcgtaaagcg taggaaacaa
    22801 ttccaacaaa gagttgtcac gcagcgaagc ttgcgtcaat taaatcgaac aattaatgca
    22861 catgaagaaa aataccacgc aagtaaagca tatgaagagc aacagcgaag aatccaaatg
    22921 gaacaagaaa gacaatagga ggaagagaaa tgaaacaata tttagatcaa tggaaagtca
    22981 tagaaggatc actaaaagaa gaacgaatcg aacaacttcc tgattgttta gaaaaagagc
    23041 atttgtttca gataagcgaa atgttgagaa acgaacagtt tgatccaaat caatttttag
    23101 ttgttgagta cccagctact ggtgtgtatt gttgtaatca tgtgaagggt gaaaaatatt
    23161 ttattattca agaatacgaa ggtaagttag cgccttatta tactacttgg gaaataaatg
    23221 aagagggaat caataatttc ccatgtgaaa gcattgaaga gtctatttct ctcacggaat
    23281 gttaaataaa tagagacgta aaagagtcct gaacgtgatt gtccaggact ctttttcaat
    23341 gaattgtttt ccgccaattc actgagtggt gcgattcaat tttattatat caaaagaaag
    23401 tgagattcca aagtgaaggg aggaagaaga aatgactcag acaacgattg gtgttgtggt
    23461 tgaaaaaagc agggataatc tcgtttttgt gacagaaatt caaacaggta gagcttttgt
    23521 agtcacagac aaagctgcaa aagcgtatca aaatggcaat attttaacgt taaatctgac
    23581 tacgaagatg ttcgttgatg cagtagaaaa ttatccattt gtttagacaa acgatatttt
    23641 acaaggagga aacggacatg gcagaagaaa aagaaccact agataatagt cggttgggaa
    23701 agtccaaaag aaaattagta cggttgcaaa acgaattaaa taatcaattt caaaagctat
    23761 ttgatcacca aaaattgaca aatgggcaac caatgaatga caaacgaaat ggtcgttcat
    23821 ggtttaacca gcaagaaaaa atagaaaata aaatacaaag tttgagagaa gagatcaagc
    23881 aacaagaaaa acaggtagaa aaacttgaaa gacaagaaga gctaaaggaa atgggctata
    23941 acaagtacgg cggactggat atgacgatcg ataatattcc aagaatcaaa gaagaaatcg
    24001 aacgctttga aaaaggtgaa tcgacttttt cagccgccac gatccgaaaa tatcaacgga
    24061 agttggaaac attagaacag ctgaaagaaa aatcagaaaa aggaaaagaa aatatcctcc
    24121 ctgaagtaca agcgatcatt gacagcggac gagtgacgca atggaaaaaa aatccaacca
    24181 tttatttttt gaaaggctat cgaaaagttg ctttagaact gaatgaaaac gggggatttg
    24241 aggaatcaaa aaaataccaa gccacgtcgg aagaggaaaa agcagctgtt cgtgaattac
    24301 ttgaagaggc tgcaccaact gattctctga aggagaaaga aaaagatcat ttctatcgag
    24361 tagaattcaa cgaaaatgat ccagaaaaag gattgaaaag ttatggtggg gagatcgtaa
    24421 cattagaatt gatcgatcaa cttaaaacgt tggataacca atacgctgca gaagcaggtt
    24481 attataaatt ctattttgaa gaagtgaaga acggagaagt agtagatcag atccgaatgg
    24541 atctagggga tggcgtcaac agtaatcacc ccatctattt agagttagca aaattgtgcg
    24601 aacaagtacc tgagaaggaa caaaaacaat cggtcaaaaa tgaacctgtt tcgaaagaac
    24661 aaatgcagtc gaaagaaaca aaaacaaaac aaaagggcta tcaaaaacga acggcagaag
    24721 agatcaaaca agaagttaaa tccctttcgc aacaggcact ggatgcggtg aaaaagcata
    24781 cccattcttc tgaagacgtc aaagaacttt tgaactttat gtctcgtttt ccagaacgat
    24841 cctttagaaa tcaattattg attgaggaac aatttccagg tgcaacggct tgtttaggtc
    24901 gtgccgcgtt gaaaaaagaa ggaatctata tcaaaaaagg ggaacaaggg aacaagatct
    24961 ttgtcagaaa aacggtcaaa ggattttatg agaaaggacg tggctttgtt cgagaaacag
    25021 aagctacacc agaagataaa aaacgaatcg aaagtggtca aatcgaagtg atcaaaaaac
    25081 cttactacac gattgaaaaa gtgtttgata tcacacaaac acagttaaaa cctgaagact
    25141 atccgaaaat ctttccgaac cgtgtctttg attttcaatt ggataaaaaa ggacagtcag
    25201 aacttcaagc tgggattcaa gcggtagcca acaatattgg aatacaaatt cgagatatga
    25261 cagaaagtga agtctatcaa agagaattag gacaagcacg tggggcgtat gttcgtaacg
    25321 aatttactgg cgaagaagaa atcgtcatga atactcgaaa tactc
//
LOCUS       DACGLW010000039        25363 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.039, whole genome shotgun sequence.
ACCESSION   DACGLW010000039 DACGLW010000000
VERSION     DACGLW010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25363
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.039"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            complement(137..514)
                     /locus_tag="IXT53_001568"
     CDS             complement(137..514)
                     /locus_tag="IXT53_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905398.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            770..1498
                     /locus_tag="IXT53_001569"
     CDS             770..1498
                     /locus_tag="IXT53_001569"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HAQ4905399.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            1498..1752
                     /gene="purS"
                     /locus_tag="IXT53_001570"
     CDS             1498..1752
                     /gene="purS"
                     /locus_tag="IXT53_001570"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010758866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HAQ4905400.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1754..2425
                     /gene="purQ"
                     /locus_tag="IXT53_001571"
     CDS             1754..2425
                     /gene="purQ"
                     /locus_tag="IXT53_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HAQ4905401.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            2426..4648
                     /gene="purL"
                     /locus_tag="IXT53_001572"
     CDS             2426..4648
                     /gene="purL"
                     /locus_tag="IXT53_001572"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HAQ4905402.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4633..6072
                     /locus_tag="IXT53_001573"
     CDS             4633..6072
                     /locus_tag="IXT53_001573"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HAQ4905403.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            6104..7147
                     /locus_tag="IXT53_001574"
     CDS             6104..7147
                     /locus_tag="IXT53_001574"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HAQ4905404.1"
                     /translation="MANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGFGGC
                     FDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEPLYF
                     LDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFAVGI
                     AEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKKTLG
                     EELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSWPIL
                     PIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGKVIA
                     KEKHRVVFIGEER"
     gene            7144..7722
                     /gene="purN"
                     /locus_tag="IXT53_001575"
     CDS             7144..7722
                     /gene="purN"
                     /locus_tag="IXT53_001575"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HAQ4905405.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7719..9254
                     /gene="purH"
                     /locus_tag="IXT53_001576"
     CDS             7719..9254
                     /gene="purH"
                     /locus_tag="IXT53_001576"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HAQ4905406.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            9270..10544
                     /gene="purD"
                     /locus_tag="IXT53_001577"
     CDS             9270..10544
                     /gene="purD"
                     /locus_tag="IXT53_001577"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HAQ4905407.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10666..11580
                     /locus_tag="IXT53_001578"
     CDS             10666..11580
                     /locus_tag="IXT53_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HAQ4905408.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11713..13278)
                     /locus_tag="IXT53_001579"
     CDS             complement(11713..13278)
                     /locus_tag="IXT53_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ4905409.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(13271..14722)
                     /locus_tag="IXT53_001580"
     CDS             complement(13271..14722)
                     /locus_tag="IXT53_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ4905410.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14736..15752)
                     /locus_tag="IXT53_001581"
     CDS             complement(14736..15752)
                     /locus_tag="IXT53_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ4905411.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15972..17060
                     /locus_tag="IXT53_001582"
     CDS             15972..17060
                     /locus_tag="IXT53_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ4905412.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            17114..18640
                     /locus_tag="IXT53_001583"
     CDS             17114..18640
                     /locus_tag="IXT53_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HAQ4905413.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            18642..19847
                     /locus_tag="IXT53_001584"
     CDS             18642..19847
                     /locus_tag="IXT53_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ4905414.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            20104..21081
                     /gene="guaC"
                     /locus_tag="IXT53_001585"
     CDS             20104..21081
                     /gene="guaC"
                     /locus_tag="IXT53_001585"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HAQ4905415.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            21345..22769
                     /locus_tag="IXT53_001586"
     CDS             21345..22769
                     /locus_tag="IXT53_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HAQ4905416.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            23230..24339
                     /gene="pdhA"
                     /locus_tag="IXT53_001587"
     CDS             23230..24339
                     /gene="pdhA"
                     /locus_tag="IXT53_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HAQ4905417.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            24342..25319
                     /locus_tag="IXT53_001588"
     CDS             24342..25319
                     /locus_tag="IXT53_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HAQ4905418.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
BASE COUNT         8205 a         4317 c         5696 g         7145 t
ORIGIN      
        1 ataaccaaaa aagaagcaga tccgttagcg gttctgcttc ttttagttta ttatattttc
       61 ttatcaataa taggaacctg attttaaaag cggctacctc tttaccgtgc ctctttacat
      121 acaaaaaata gaggaattaa gcgtaaatgg cttggctatt ataactaaaa tgctggttat
      181 ggagatgttg cagcaagttt tcgagaaaag cgtattccat ctcttgtcca ttgaataaga
      241 tctggatacg ataatgattt ttgaaacgct tctgttcatg aatctgactg attgaaagtt
      301 cgcctttgtg ttctgtctga taaagaaaag tccttttacg gcattcagca cagcgcttgg
      361 ttgctcggcg gatttttcgg aatgtataaa aactagtatc tttattgcat aaataaacgt
      421 ctttgatcga cagcaaatca ttatatagac aggaacgttc ttcagctgtt aattctgata
      481 aaaaaagctg accttgtttt attgcatctt ccatcggcaa gtgcctcctc gaacccatta
      541 tccaaatttt tagttaattc taatatacca tcattaagta taaatgtaaa cgcttgttga
      601 tggtataaga ttttcgtttc tgataaaaca cgaacattaa aagaatctaa tgccttaaat
      661 gtacatattt ttgttgattt ataaaaagat gattgctaaa atttaaagtg aaaaaacgaa
      721 caaaaaacga tgtatttgct tttaatgtaa ataaaaagga gagacgtaca tggagaagaa
      781 agaacttttg tatgaaggaa aagccaaaaa aatatatctt acttctgatg agaacttgat
      841 ttggatagaa tatttagatc aagcaactgc gttgaatggc gcgaggaaag accagataag
      901 tggaaaagga gaattgaata atcaaatcac aagtgcaatt ttccgttatc tgactcaaag
      961 aggaataaaa aatcatttta ttgaacaatt gtctaaaacc gaacaattag caaaaaaagt
     1021 acaaattatc cctctggaag tggttatccg aaatattgca gcaggaagtt tctcaaaacg
     1081 tctaggtgtt cccgaaggta aagaattcat caaaccaatc attgaattct actataagga
     1141 tgaccagttg gatgatccat ttatcaatga agagcatatc tatttcttac aacttgcaaa
     1201 gccggaagaa atcagtgagc tgaaagccca agcgttggct attaatgaaa ttttgaccga
     1261 attatttgct aaggtcggtt tggttttagt agatttcaag ctagaatttg gccggtcgaa
     1321 tggacaaatt ttgcttgcgg atgaagtaac accggatact tgccgattat gggatgaacg
     1381 tacgaatgaa catatggaca aagacgttta ccgcagagat ctaggtgatt tgatcccagt
     1441 ctatcaagaa gtatataacc gtctatcgga actaactgaa gaaaaggagc gagtatgatg
     1501 tatttcgtaa aagtatatgt aacctataaa gattcagtgt tagatcctca aggggaggca
     1561 gtaaaaaaag cagtccatcg tatgggatat gaaacaatcg aagatatccg aataggaaaa
     1621 tactttgaga tacaacttgc tgatgcagaa gaaccaatcg atacagtgat tgaaacgatt
     1681 tgtgacaagc tgcttgcaaa tgtaaatatg gaaacatacc gatatgaaat caaaaaaatg
     1741 gaggaagtat aacatgaaat ttgcggtcat cgtttttcca ggttcaaatt gtgacgcaga
     1801 tatgttatgg gcagttcgcg agatcatggg cgctgatgcc gaatttgtac ggcatgatga
     1861 gtcctcttta gaaggatttg atggggtttt actgccggga ggattctcct atggtgacta
     1921 tttgagatgc ggtgcgattg ctcggttttc tacaatcatg gaagaagtga ttcgttttgc
     1981 tgaagaaggg aaaccagtat tcggtacatg caatggattc cagatattga cagaagcagg
     2041 acttttgcca ggtgtactga gacgaaatga gtctttgcat tttatttgca aaactgtacc
     2101 attgaaagtc aaccccaaga cggcctttac ttcagagtat gaagccgacg aaataatcta
     2161 tcttccagtt gctcatgggg aaggaaatta tttctgtgac gaagaaacac ttcaagaact
     2221 aaaaaaaaat aatcagattg cctttactta taatgacaac atcaacggca gcgtggaaaa
     2281 cattgcaggg attacgaaca aagcagggaa tgtgttagga atgatgcctc atccagaacg
     2341 tgcagtagaa gacttgctag gatcagaaga tgggaaacgg ttctttgctt ccattttaaa
     2401 aaacttcgga aaggtgaccg tatagatgaa aacattgaaa gaaccgacag ctgaagagat
     2461 caagaaaaaa agaatctatg ccgaatgggg attgactgat gaagaatatc gaatgatcga
     2521 agaagatatc ttaggaagaa tgcctaatta tacggaaact ggactgtttt ctgttatgtg
     2581 gagcgaacat tgttcctata aaaattctaa accggttcta cgcaaatttc ctactagtgg
     2641 acctcatgtg ttgcaaggcc ctggagaagg cgcaggcatc gtcgacatcg gtgatggact
     2701 agcagttgtc ttcaaagcag aaagtcacaa tcatccttca gccgtggagc cttatgaagg
     2761 agctgcgaca ggagttggcg gaatcatcag agatattttc agcatggggg ctcgtccaat
     2821 tgcgttgcta gattcattga ggttcggcga attagataat ccaagaacga aatatttact
     2881 agaagaagta gttgcaggta tcggcggata cggcaattgt atcggcatac cgactgtagg
     2941 cggcgaaatt gcatttgatc cttgttatga aggaaaccca ttagtcaatg caatgtgcgt
     3001 aggtttgatt gaacacaaac atatccagaa agggcaagcc gctggcgtcg gtaattcgat
     3061 catgtatgta ggtgcaaaaa caggtcgcga cggtatccac ggagcaactt ttgcctcaga
     3121 agaatttaat caagaagaag aacagcaacg ttctgctgta caagtaggcg atccttttat
     3181 ggaaaagctg ctgcttgaag cgtgtttgga attaatcttg catcattcgg attctctaat
     3241 aggtatacag gatatgggag cagcaggact tgtttcatct agtgcggaaa tggcttcaaa
     3301 agctggctca ggacttatat tgacattaga tgaagtccca caaagagaaa ctgggatgac
     3361 accttatgaa atgatgttat cggaatcaca agaacggatg ttgatttgtg ttaaatcagg
     3421 agaggaagaa aaaatccaag aactatttca gaagtatgat ctagatgcag tcactatcgg
     3481 taaagtaaca gacgacgggc aatatcggct atatcatcaa ggagaagaag tagcaaatct
     3541 gccagtcgat gcattagctg aagatgcacc agtttatcat aaagaaatga aagaaccagc
     3601 tcgaatcgta gaatttcagc agttagctcc ttatcagcca gttatcgaag aacctggaca
     3661 agtattgctt gatctgctgc agcaaccgac gattgcttca aaacgttcaa tctacgaaac
     3721 ttatgattca caagtacaaa ccaatacagt tgtccgtcca ggaagcgatg ctgctgtgat
     3781 gagggttaga gggacgaata aagcgttagc tatgaccaca gactgcaacg cgcgctatct
     3841 gtatttggat ccaaaaatcg gaggacaaat agcagtggca gaagctgcta gaaatattat
     3901 tgctagcggt ggtaagccct tggcgatcac cgactgcctg aactatggtt ctcctgataa
     3961 accagaagtt ttttgggaat tatcgacttc agctgacgga attgctgctg cgtgtgaaac
     4021 gttaggaact cctgttattt caggaaatgt ctctttatat aatgaaacgg atggtcaagc
     4081 aatctatcca acaccaatga ttggtatggt cggattgatt gaggatcata aacatatcac
     4141 tactcaggaa ttcaaaaaat caggggatct aatctatata ttaggtaaga cgtttgcaga
     4201 ttttgatggc agtgaattac aaaagatgca gcttggaaga atcgaaggtg tgatccgaaa
     4261 ctttgatcta tccattgaaa agagaaacca agagttagta ttgacagcca tccaaaacgg
     4321 attgattgaa agtgctcatg actgttcaga aggtggcttg gcaattgcac tggcagaatc
     4381 agctttcaaa catcagctag ggatatctgt acagtttgaa ttgtcctctg ctcaattatt
     4441 tgcagaaact caatcaagat ttgtgttgac agtcgcaccg gaaaataaga cacgattcga
     4501 agaaatgatg ggagatgctg ctgttttagc aggtaaggtg acggatgaag cgataatcga
     4561 aatttcagca actgacggtc agatcaaaat agaaactgca gtagcaagaa agtgctggga
     4621 ggatgcaatc gtatgtctta tgaagtgaag agtttaaatg aagaatgcgg gatcttcggt
     4681 gtttggggtc atccagacgc cgctagtgtg gcatacttcg ggctccatag tcttcagcat
     4741 cgaggacaag aaggtgcagg tatcgtgtcc aatgatcgag gaaagcttaa ggggcatcga
     4801 gatttggggc tgctttctga ggtattcaaa gatcaaagaa aattgcagca gttaacagga
     4861 gaagctgcaa tcggacatgt acgttatgca acagcaggca atggaagtgt ggacaatatt
     4921 cagccttttc tattcaaatt tcatgatcag tcgatcggct tagcgcataa tggtaacttg
     4981 atcaatgctc gttcccttag aaaagagtta gaggaaaatg gggcgatctt ccattctaac
     5041 tccgatacgg agatattgat gcatttgatc cgacgaagtg aagaaacaga ttttttaaat
     5101 aaattaaaaa gtgctttgaa tgaagtcaaa ggtggctttg cctatttatt aatgacagaa
     5161 acggcaatga ttgccgcttt ggacccaaat ggttttcgtc cattagcaat cggacaaatg
     5221 aaaaacggag catacgtgat tgcctcggaa acttgtgcat tagaagttat tggggcacgt
     5281 tttataagag atgtccagcc tggtgagata gtgatcatca atgacgaagg aatccagatt
     5341 gattgcttta cgaaggacac gcagctttct atttgttcga tggaatatat ctattttgcc
     5401 agaccagatt caaacatcgc agggatcaat gtgcatactg cacggaaaaa tatgggacgg
     5461 aatttagcga aagaatcgcc agtaaaagca gatatggtcg ttggcgtacc taattcttct
     5521 ttgtcagccg caagcggtta tgcggaagct agtggaatcc cttatgaaat cggattagta
     5581 aaaaatcaat acattgcccg aacatttatc cagccaacgc ctgagctaag agagcaaggt
     5641 gttcgaatga aattatctgc tgtacgagga gtagtggaag gtaaaaaggt gattatggtc
     5701 gatgattcaa ttgttcgggg tacgacaagc agacggatcg tccatctttt gaaagaagca
     5761 ggggcaaaag aagtccatgt gaggattgct tctcctcctt tgaaatatcc atgtttttac
     5821 gggattgata ttcaaacgcg taaagaactc attgcggcaa atcacacgat tgatgaaatc
     5881 aaagaatgta tcggtgcaga ttctttaggt tttttaagtg aagagggtct gatcgaatcg
     5941 atcggtctgg aaaaagatgc tccgtattca ggattgtgta tggcttattt caacggagat
     6001 tatccgacgc cgttatatga ctatgaagaa aaatatcagg aatcactaaa agaaaaagta
     6061 tcgttctttt aaaagcagac caagaaggga gtatatgaat tcaatggcaa atgcatacgc
     6121 aaaagctggc gtagatgtcg aagctggata tgaagtagta gaacggatca agaagcatgt
     6181 caaaaaaacg gaacgtttag gtatgatggg cgctatagga ggattcggcg gttgttttga
     6241 tttatcggtc tttcaattaa aagagccagt ccttgtctcg gggaccgatg gcgtggggac
     6301 gaaattagca gtggcaatcg aagcagacaa acacgataca atcgggatcg actgcgtagc
     6361 gatgtgtgtc aatgatatct tagcacaagg cgcagagccg ttatatttct tagactactt
     6421 agcaacgggg aaaaatgatc cgttacgttt ggaaaatgta gtaaaaggtg tagcagatgg
     6481 atgtgttcaa ggaaatatgg cgctgattgg cggagaaaca gctgaaatgc ctggtatgta
     6541 ctcaggtaat gattatgatc tagccggatt tgctgtaggg atagctgaaa aatctcaatt
     6601 attgacagga gaaacgatca acccaggaga tgtgttgctt ggaatagcat ctagcgggat
     6661 tcattcaaat gggtactctc ttgttcgcaa aattttcttt gagcagcagc agttttcttt
     6721 atcatcaaaa ctgccagaat tgaaaaaaac acttggcgaa gaattgcttg agccaacgaa
     6781 aatctatgtt tcttccttgc ttccgctgat caaagaaaag aaaataaaag gagctgcaca
     6841 tatcactggt ggagggtttg tagaaaatat cccgcgaatg ttaccagaat ctatggctgc
     6901 acaaatcaaa cttggctcat ggccgattct tccaatattc ggctgtttac aaaaatatgg
     6961 tgaaattcct gagatggaga tgtatgaaat cttcaatatg ggcatcggta tggtcctggc
     7021 agtcgaagaa tcgaaagtaa aagaaatagt acacgaaatt gagcaagctg gcgaaaaagt
     7081 ttatgaaatc gggaaagtga tcgccaaaga aaaacatcgt gtggtgttta taggagaaga
     7141 acgatgagaa tcgctgtttt tgcttctgga aacggaagca actttcaagc gctagccgat
     7201 tatctatcga aaaaaggatt ggaatcttcg attgactggt tgttttgtga tcagcctgaa
     7261 gcatatgttt tgaaacgtgc aactgcgttg agtgtaccgg cagattgttt ttcacctaaa
     7321 gaatttgatt caaaaaaaga gtatgaagaa gcaattttgc ataaattgaa agaaaaaaag
     7381 atcgatttga tcgtattagc tggatatatg cgaatcattg gaccggtatt gctgaaaaat
     7441 tatgataaaa gaatcatcaa tatccatccg tctcttttac ctgccttccc tggtttgcat
     7501 ggcatacgtg atgcctttga agctggagta aaagaaacgg gtgtcacaat tcattatatc
     7561 gaccaaggtg tagatactgg accaatcatc cggcaggaaa aagtgagaat tgaacaggag
     7621 gacacgttcg attctttaga agaaaaaatc catagagtag agcatcgaat ctatccagaa
     7681 gtgattagtg aaattattga aaatggaggc tatctgaaat gacaagagca ttgatcagtg
     7741 tatcagataa aactgggatt gtagcattcg cccaaggttt gattgaacaa ggagtagaaa
     7801 tcatttcaac aggaggaaca aagcatgcgt tggataaagc tgggatcaaa acgctttcga
     7861 tcgaagaagt gacagggttt cctgaaatga tggatggccg tgtcaaaaca cttcatccta
     7921 aaatccatgg tggtctttta ggaagacgcg atctccctag ccatatggag gcaatggaaa
     7981 cggaaaatat ccaaccaatc gactttgttt gtgtcaattt atatcctttc aaagaaacga
     8041 ttatgaaacc agatgtcgaa atagcagaag ctattgaaaa cattgatatc ggtggtccta
     8101 gtatgctccg tagtgcagca aaaaaccatg aatttgtcac ctctgttgtt gatccatctg
     8161 actatgaaga agtactttca gaattacgtc agtatgggca tacttcactt tctttcagac
     8221 aaaaactagc ggcaaaagtc tttcgtcata ctgcggcata tgatgcactg atcgctcaat
     8281 acttaacgga ttgggtggaa gaaaaagaac cggaaaaatt gacacttacg tatgaacgaa
     8341 aacaaacgct tcgttacggt gaaaatagcc atcaagccgc tgccttttat cagtccgctc
     8401 ttctggaaaa atattcgatc gcagcagcaa aacaactcca tgggaaagaa ctttcttata
     8461 ataatatccg agatgcagat gctgcgctac ggatcatgaa ggaatatgac gagccaacgg
     8521 tagtcgcctt gaaacacatg aatccttgtg gtattgggac agcatctacg attttagaag
     8581 cgtgggaagc agcgtatgaa gcagatcctg tttcaatctt tggtgggatc atcgttttga
     8641 atcgagaagt agatctgcca acagcagaac agatgcataa gctgtttcta gaaatcatca
     8701 ttgccccttc ttatacacaa gaagctttgg aattactgca aaaaaagaaa aacatccgat
     8761 tactgacagt agactttgcg caggaagaaa atcaaaatcg agaagaaacg gtctcagtct
     8821 taggtggact tcttgtacaa gatcaagatc tggcaataga aaatgaagaa gagtggaaag
     8881 tcgtgacaaa acgtaagccg actccagcag aagcaaaagc attagcgttt gcttggagag
     8941 ctgtcaaaca tgtgaaatct aatgcgatcg tactggcaaa cgaacgtcag acagtaggaa
     9001 tcggagccgg gcagatgaat cgagtcggtt cagtaaaaat tgcgattgaa caagcgcaag
     9061 agaaaatgga aggtgcagtt ttagctagcg atgcttattt tccaatggat gattcggttg
     9121 aatatgcagc caaacatggt ataaaagcaa tcattcagcc aggaggaagc atcaaagacc
     9181 aagattctat tgatatggcg aataaatacg atctagcaat ggtctttact gatgttcggc
     9241 attttagaca ttagagagga gtcggataaa tgaacatttt ggtgatcggt gcaggtggtc
     9301 gtgaacatgc aataagtaaa aaacttttag ccagtccgaa ggtggatacg gtgtattgtg
     9361 cgccaggaaa tccggggatg acaaaagatg gtatcaattg tgtcgctatc agtgagcgag
     9421 atcattcagc gttaatccat tttgttaaga aagaggctat tgattggagt atcgttggtc
     9481 ctgaagttcc tttgttaaat gggatcgtcg atgattttat ggcggaagga ttgaaaatct
     9541 tcggtccgac aaaagcagca gctctgatcg aaggttcgaa aagttttgct aaacaattaa
     9601 tggatactta tcaaatcccg acagctaaat ccagaagttt tgaatgttac gaaaaagcag
     9661 cagcgtatat caaagaaaat ggtgcgccaa tcgtagtgaa agcggacggc ttagcagctg
     9721 ggaaaggtgt agtcgtggct accacagtga aggaagcgct ggatgcggca gaacaaatgt
     9781 tgaaccagca tcgatttggg gatagtagca aaaaaatcgt cgtagaagag tttttggcag
     9841 gagaagaatt ctcccttttg gcgtttgtcc gtaataccaa ggtgtatccg atggtcattt
     9901 cgcaagatca taagcgggca tttgataacg ataaagggcc aaatacaggc ggaatgggtg
     9961 cttattcacc agtaccgcaa atttcaaaaa agatgattga aaaagctgtg gaagaagttt
    10021 taaaaccagc ggctttggga atgaaagaac taggacgctc tttcacgggt gttttatatg
    10081 cgggtttgat tgctacgaag gaaggaccaa aagtgatcga attcaatgcg cgtttcggtg
    10141 atcctgaaac gcaagtcgtt ctttctcggt tgaagagtga ctttgcgcaa gtaatcgatg
    10201 atttattaga gaatcgtaca gtagagttaa agtggcagca agaaggatac aatgtaggcg
    10261 ttgtggttgc agctgccggt taccccgaag aatatgagac aggaatggtt ctaccagatt
    10321 ttctggaaga agagctttct gtttactatg ctggagtttc ctcaaataac ggcaaactag
    10381 ttggagcagg aggacgtctt tatttagtag aagcatacgg tgagacgatc aaagaagcac
    10441 agaaaaaaat ttacaccgcg ttaatggata aaacgacaga tggaacattt tatcgtacag
    10501 atattggaga aaaagctgcc aatagcgaac tgatcgtgaa gtaaatcagc tgcttccatt
    10561 tataaaaaat acaacaaaca taatcggaag cgccgatttt agtgaagcct tttcataaag
    10621 agtgtggtac acttttgttg tgtatataaa taaggaggag ttcagatgaa aaaaattcaa
    10681 ttaggtactt ctgatcagaa agcagctgca gtgatcttag gctgtatgcg tatcaacaat
    10741 gcggaagatc cagtaaaagt gattgagaca gcttatgaaa acggcattga tttttttgac
    10801 catgcagata tttatggaaa cggtgaatgt gaagaaatct ttgcagatgc tctagcaaaa
    10861 acatcaatca aacgagaaga tctttttatt caaacaaaat gtggaatcgt tccaggtgtg
    10921 atgtttgatt tttctaaaaa acatatcatt gaatctgtag aaaaaagttt gacccgtctg
    10981 aaaatggatt atgtggatgc tttacttctt catcgtccag atacattggt agaaccagag
    11041 gaagtagctg aagcgtttga tgaattggaa cggtcaggaa aagttcgtta ttttggcgtc
    11101 agcaatcaaa aaccaatgca aatcgaattg ctgaaaaaat atgttcgtca accattagta
    11161 gctaatcagc tgcaatttgg cttgaaacat actggaatga tcgatcaagg gattcatgtc
    11221 aatatgacag atgaagcaag tatcgatcat gacggtagtg tacttgatta ttcccgattg
    11281 catgatatga cgatccaagc atggtcacca taccagtatg gattttttga aggtgtattt
    11341 atcgggaacg aaaaattccc tgaattaaac caaacattag aagaactagc agctaaatat
    11401 caaacgacac caactggctt ggcttctgct tggattttac gccatccagc caatatgcaa
    11461 gtgatcgccg gtacgatgaa cgtagggcga atcaaggaaa tcgctcaagc ttcagaaatt
    11521 aatatgtctc gggaagattg gtacgaattg taccgtgctg ctggaaacat tttaccttaa
    11581 agattgtcat aagcaggtca aaagggctat aaattaaaaa gacagaataa cttgaaagct
    11641 gttttcagct tcgagatatt ctgtcttttt ttattgcaaa tggaaagagt atcattgtac
    11701 ggatttcgtt tattagttat ttttgttttt aaggaaaagc tgtgtccgat attcttttgg
    11761 agaaatccct tgttttttct taaataaata actgaaatag ttagggtcat tataaccaat
    11821 ttcaaaagca atatctgaaa tacgatagtc tgattgctgc aataattttt ttgctaattc
    11881 gattctttgt tctgtcagat aatcgatgaa tgtttggtta aacgattgag aaaaaatcgt
    11941 actgaaatga gagggactta atgcaacttg ttgagcgacc caagttaagg acatatctgg
    12001 atcggtaaaa ttttctttga taaaatccaa tgcatggttc aaaattggct gatatttgtt
    12061 ccttgaaggc tgtgagggca gagcagttaa gaaagaaatg aacatataaa ctgtcgaacg
    12121 gtaaagcgag tcgtcggcgc tgattgctaa gcgttgactg gtggatgtgc aatgaaccat
    12181 ttcttgcggg attttctttt ctttcaggca gacatgctgg agtagcttag tcaagacaaa
    12241 taaacgatat aatcgtgttc gagttggtgt gccttgtggt ttcgtcaagt gttcgatgta
    12301 cttagatact tcgtttttcg ataatgaaga caaatcaagt gagaggtcaa agtaaaagac
    12361 agaatcaact ggaagtttcg cttcttgaat caaatcctca tagctgataa tttgttcttt
    12421 aagggaactg gcggtttcaa gtatcatctg cgtgatctca aaagatgcag gtaattcgct
    12481 gattctttct gcgacaggac caatggcgac agaaaaatta tttttttgtc ccttttctaa
    12541 ttcatggacc aacgtttggg ctacctgata actggtctcg atcacatttt cttcaagaga
    12601 atcaactatt aaaaatttga tgaatcgaga ggaaacagca gagaagatca cttgttgatt
    12661 ctccctaaat aaaaacttca aataatcact gagatgagcg taatcttcaa agtctgtact
    12721 gacttggttt gctgctaata atacggtcat ttttttccct gctaaagagc gattgaaaga
    12781 ttcagcaagt ttcaatgttt cactgatcgt aagtgagcct ttgaacaaac cgttcaagaa
    12841 atgatttttt ttcaattcga aaacaagatc agaagaagtg tgcgtttctt tttgctgcaa
    12901 atctaaagca gttactgctt tttctattgc ttgtttcaat tcgctacttt taatcggttt
    12961 aagcagataa tcatctgcct gtacctggat tgctcctttc atataatcga aatcgtcgaa
    13021 tccactaata aatatgagac gagtccaagg aagtagtttc cttgcttctt ttgaaaaagt
    13081 caacccgtcc atgaaaggca tttggatatc agtcagaatg atgtcgggct gtaagtcgat
    13141 aattgaagac agtgccatct caccatctcc agcttcgccg acataagtga ttggataata
    13201 agaaccaagg acagttattt gttcccgcaa gttattgcga atgaggtatt cgtcttcgat
    13261 gataaaaact ttatacatgt ttgtttcctc caattgtttt gggagataga gggatcatca
    13321 gtgtgacagt tgttccttct cggtatttgc tgtcaatcgt gataggtttt tgatcatttc
    13381 cgtaataaag aaggagacgc ttgtttacgt tgtaaagtcc gtagccgata ctgaaatctg
    13441 aatcgatccc tttggctaac tctttacgta tttctgccag tttttctgca gggatgccaa
    13501 tcccgttgtc tataactttg aattggatca tctcatcaat tttgcatgtg gtaatacgaa
    13561 taaatccttt gtgacggacg tacttcgttc catggtatac agcattttca atcaatggct
    13621 gcaagatcat ttttaaaata gaaacaggaa aaagtgtttc tggtatatcg atcttatagt
    13681 caagcatatg gccgtatctt atttttaaga tgactagata atcattgata tggctaattt
    13741 ctttttcaag aggaatccag tcttgtcctt tgcttaaaga cgtacgaaaa aagccggaaa
    13801 gtgcatgggt catagtcaga gcttcttcaa agcagtcttg ttcgatcaaa gaaataatgg
    13861 catccaaact gttataaata aaatggggag taatctgagc ttgcaataca cgaacttcac
    13921 tttgtgctaa atggtattgt ttcaaagcgt tctcccgtaa agactggttt agatcttccg
    13981 ccattttatt catgctagcg gtaagtgttt gtaattccag tgtttccggc agggttaaac
    14041 gaaagccaag gttccctgaa gaaagttgtt ctgacatctc gatcagctga ttgatcggtt
    14101 cttggacctt tctctctaaa aagcgacgat tctttttcgc atagaaaaaa atggtactaa
    14161 gaactccaag ttccactatt gttaatagaa tgagcgagcg gacaagtgtc gtattttttt
    14221 tgcttgctaa gtcgatctcc acacgaacaa aatcttgtaa gatatctgac agcagttgag
    14281 tgacagaatc tacctgattg atgatctctt cattggtatc cataaaatct tttgccaaga
    14341 tattattgat cagctcgttt tgataatctt ccagtgtatc gatcgtacgt agcgatacat
    14401 ctaacgtact aatttcttta agtgtatttg tattattttt gatctctcga atatcattcc
    14461 taagttcttc taggatattt tcctcattga actcatctac ggttacttgg ccagaaatca
    14521 agtcccacat actttccaaa actttttgat caaccttcaa agcaattgca tctgcttttt
    14581 ggatatttgc gatcgtctgt tgataaatca aaagctgttg cgaatataac agtatgctca
    14641 gtagcaaagg gataacagca aatagaagca tggctttgtt tgcttgggca aaaatctgtt
    14701 ttatgcttaa attttttgtc atgatacttc tccagtcaat aatttctggt tgggatgtta
    14761 gctatattct gctgggaaaa gacagtctcg gaaataaaaa tatctttagg taatgttttt
    14821 cctgtttgaa tatttccaaa ataacgagta acggtattgg ctacatacca gccgacatca
    14881 ggtttacact cgactacaca attgacgatt cctgagcgta gcttctcgat catatcttct
    14941 tgtccatcaa tagttacaat gatcaaatct tctccaggtt tgattgatgt ttcttttatc
    15001 gcagctaaag caccgtgagt catttcatca ctatggctgt ataatacatc gatttcggat
    15061 aaatcatttg tttctaaata tcgtttcatc tggtcttttc ctttcatccg aataaaatca
    15121 ccgacaattc ggtgaacaat agtcatcgga ggtttatggt cgatggttcc atcttttttt
    15181 agggttggtt tacgattgat tgattccaaa aatccttcgg agcgtagctt ggtcggtgtg
    15241 gagttctctg aaccagcaag ttctaggatt ctgatttcct gtttctttga atttgtgaag
    15301 tgattgctga caaataaacc cgcccgattt ccttctgctt tgaaactggg gccaatgtgg
    15361 gtgacgaaaa gctcgggatc attcgtttga atatgtctgt cgacaagtat gacagggatc
    15421 cctgctcgct ttgcttcttt taatacagag tcccacccgg attcttgcaa aggagtaaaa
    15481 atgatcatat ccactttata ggcaatgaac gtccgtatat cttggatttg ccgttcctga
    15541 ttcatgtagc catttcggta gagaacttgg taatcttctt tttcaagcgc agtgataatt
    15601 gattcagtat gctttttccg ccagctactc tccgttcctg attgcgaaaa accaacgact
    15661 agtgggtcag gtgcttcctt tgtattaggt gtacacccca tcatttggta agcgattaca
    15721 acaagggcta aaaaaaataa aacacgtttc actttaaatc actccatctg gatttgttag
    15781 tactatctta tcagataaaa gggaaaatca aagtcaaaag gaatcaatcg aaaaaaatcc
    15841 aagaaactta aaaaaatcca agacaaaaaa agtacaccta aaaaatttaa agtaaatccc
    15901 ataaaaaaat caatgttaac gctttcttta ttagctatac tcggtgtatc aattgaaatg
    15961 ggggataaaa aatgaaaaac aaatttagaa agattgttgg gactatcgct attttgaccg
    16021 caagcattct attaagtgcg tgtggcaatt caggatctgc agatgatagt acggggtatg
    16081 taggaatcgc tatgccgacc aaatcagcag aaagatggat cgctgatggg aacaacatgg
    16141 tctcggaatt agaaaaactg ggttataaaa cagatcttca atatggagaa gataaagtag
    16201 aaaatcaagt agcacaaata gaaaatatga ttacaaaagg ggtagataca cttgtgattg
    16261 cttctatcga tggttcggct ttaactgatg tgttagctaa agcaaaagaa gcagatatca
    16321 aggtgatcgc ctatgatcgt ttactgatga attcagaaaa tgtcgattat tacgcgactt
    16381 ttgataattt tggagtaggt gtatcgcaag cagcttatat tgaagagcac ttaggattga
    16441 aagaaggaaa aggaccattc acgattgaat tatttggtgg ctcgcctgac gacaacaacg
    16501 cactaatcaa ttacaacgga gtgatgtctg ttttacaacc atatatggac aacggtcagt
    16561 tggtcgtgcc atctggacag acgagtttca gtcaaatcgc tacgcttcgc tgggatggtt
    16621 ctacggcgca agcaaggatg gataatctgt taagcgcaaa ttatactgac caaacattgg
    16681 atgctgtttt gtcgccatat gatccaataa gtttaggaat catttcgtca ttaaaaggcg
    16741 tggggtatgg gagtgaaagt aaacctttac ctgtgattac tggacaagat gcgacagttg
    16801 ctggcgttaa atcgatcatt gccggggaac aaacacaaac gatctttaaa gacacacgta
    16861 tcttagcaaa aaatacgatt gaaatgatca aagcaatcag tgatggagaa gaggttccgg
    16921 tcaatgatac tgaaacttat gataatgggg tgaaaactgt ccctacgtat ttagcaaata
    16981 cagtatctgt cgataaagat aattatcaag cagagctgat cgacaccgat tattataaag
    17041 aatccgatct gaaaaattaa gaggaaccaa acaaggaact aaagagatct aagtcagaga
    17101 gaggataaga attatggcgg attgtatttt aaaaatggca gatatcacca aaaaattttc
    17161 tggaatccga gcgttagatt gcgtgaatct gatgattgaa cgtggggaaa tccatgcatt
    17221 gtgcggagaa aacggagcgg gaaaatcaac attgatgaat gtcttgagcg gcttgtatcc
    17281 atatggctcc tatgacgggg aaatctttta tgatggaaag ctgtgtcaat ttaaagattt
    17341 gaaagatagt gaagaaaaag gcatcgtcat cattcatcaa gaattagcac taagtcctta
    17401 tctttcaata aaagagaata tttttctcgg aaatgaacaa gcagaaagag gcgtgattga
    17461 ttgggaactg acagaaaaga aaacatatga actgctgaat aaagtcggtt tgtctatcga
    17521 cccgaatctt ctagtatccc agattggcgt agggcaacag caactagttg aaatagctaa
    17581 agcattatca aaatccgtcc gtctgttgat tttggatgag cctaccgctg cattgaatga
    17641 agaagaaagc gcgaatttgc tgagattgat ccgagagttg aaagagcaag gtgtaacctc
    17701 catcattatt tcacataagc tgaatgaaat cgtggatatc gcagatcgaa tcaccatttt
    17761 acgggatgga cagacgattg agacactaga aaatgaaatg atcagcgaag agcatattat
    17821 tcgcggaatg gtgggcagag atttagttga tcgttatcct gaaagaaaac cggatattgg
    17881 agaagtttat tttaaagtag aagactggac ggtctatcac cctcttgatc acgagcgaat
    17941 cataaacgac cacatcaact tgcatttgaa aaaaggagag attgtaggaa tagctggact
    18001 gatgggagcg ggcagaactg aattcgctat gagtctattt ggacattctt atggaagcaa
    18061 gatatccgga cgtatattaa aggaaggggc agagatatca gttaagaccg ttccttcagc
    18121 cattgcaaac ggactcgctt atgtctcgga ggaccgaaaa tctttaggac tgaatctgct
    18181 gatggatatt cgggaaaacg tgacgctgag cagtttggat aaaataagtc aaaaaggagt
    18241 attagataaa gaaaaagaag taactgaggc agaaaatttc cggaaaaaga tgcgtataaa
    18301 aacaaactcc atccatcaaa ttgtcagtag tcttagtggt gggaatcaac aaaaagtcgt
    18361 tttatccaag tggctgatga cacaacccga tattcttttt ttggatgagc caacccgtgg
    18421 gatagatgtt ggggcaaaat atgagatcta cacgattatc gaagaaatgg cagcaaatgg
    18481 caaagctgtc tgtcttatct cttctgaact tcctgaaata ttagggatgt gtgatcgaat
    18541 ctatacgatg aacgaaggac gtattacagg agaagtctcg cgagaagatg cagatcagga
    18601 agtcttgatg aaattaatga cgaaagaaga ggtgggttaa gttggaacag gcaaaaaatc
    18661 ttacggataa aaaagagaaa aaactaaatt tgaaaagtag agcagctgat ctttttggtc
    18721 gctacagcat ggtgatcatt ttagcgggac tacttattgc gtttcaattg atgacggatg
    18781 gtattttctg gcgtccattg aatataacta accttgttct gcaaaatagc catatattag
    18841 tactagctgc agggatgtta ttagttgtgc tcttaggtca tgtggatttg tcggtagggt
    18901 cagtgatggc gtttgttggt gcaattgcgg gcatgatgat ggtcaatcgc caaatgaacc
    18961 cgtggatgac agttatcgca tgtcttgcag taggcgggct gatcggcgca tggcaaggtt
    19021 tttgggtagc ttatgcaggg ataccggcgt ttatcgtgac tttagcagga ttgctgatgt
    19081 ttcgtggact gacacaagtt gttctaggag gccaatctct agcacctttt ccagctgttt
    19141 tccaaaaaat gtctacgggt tatttaccag acttaagcga aggaagcagc cttcacttct
    19201 tgacattgat tttaggaatg atcatcacga tcgtgttagt tgttggtcaa tggcgaacaa
    19261 gaaatcgtcg gaaaaatcat ttatttgaag tggaatctcc aacagctttc ttggtaaagg
    19321 cggtatttac agcagtcgtt gtactaggaa taacttatat ctttgcctct tatcaagggt
    19381 atccgattat tttgatcatt cttggtttga tcgttgcagc atatgggttt ttgacaaata
    19441 aaacagtagc aggtcgacag atttatgcga cgggcggaaa tcgaaaagcg gctgaactat
    19501 cagggatcaa aacaaagcga atcacgttct gggtattcgt caacatggga gtcatggcag
    19561 ctttagctgg attagtttta gctgctcgat tgaatgcggc aacacctcaa gcaggtacat
    19621 cgatggaact ggatgcaatg gcggcagtat attttggtgg tgcttcgact tccggcggca
    19681 ttggaacaat catgggtgcg attgtcggtg gcttggtcat gggtgtattg aacaatggca
    19741 tgtcgatttt aggcgtcggg gtagactggc agcaggcaat caaaggattg atcttgcttt
    19801 tagccgttgt gctagacttg tataataaga aaaaacgaac aacgtaggag aacgccggtt
    19861 attcagaaaa aagactgtga caagacattt gtcacagtct ttttttcgct aaaagcgaac
    19921 attcattgtt tcgtttgtat agactaaaaa taatatatgt ttttttacca ttcgtataaa
    19981 ttctcttttt gctcttgatt tttttgctta attgagttaa attacatagg gcgctaaaaa
    20041 aagccttgat taaattaata atgttcgtgt tttgctggat gtgaacatca ggaggataat
    20101 gtaatgaaag tatttgatta cgaagatatt caattaattc ctaataaatg tatcgtaaat
    20161 agccgttctg aatgtgatac aacggtcaca ttaggtaaac atacattcaa aatgccagta
    20221 gtgcctgcca acatgcagac gattattgat gaaacaatcg cagaattctt agcggaaaat
    20281 ggttatttct atatcatgca tcggtttgat gaagcagctc gaattccgtt tatcaaaaaa
    20341 atgaaaaagc gtggattgat tacctcgatc agtgttggcg tcaagaaaga ggaatattcc
    20401 tttatagaga agttggcaga agaatcgcta aatcctgatt atatcactat cgacatcgcg
    20461 catggacatg ctaactcagt tattgatatg attcagcata ttaaaaaata tttgcctgaa
    20521 acttttgtga ttgccggtaa tgttggtaca ccggaagccg tccgtgaatt ggaaaatgcg
    20581 ggcgcagatg caacaaaagt cgggattggt ccaggaaaag tatgtattac aaaaatcaag
    20641 acaggctttg ggactggagg ctggcaactg gcagctctta gatggtgtgc gaaagcagct
    20701 cgcaagccga tcatcgctga cggtgggatc agaacacatg gcgatattgc aaaatctgtc
    20761 cgttttggtg cgacaatggt catgattggt tcattgtttg ccggtcacga ggaatctcct
    20821 ggcgaaacaa aagtcgaaaa tggaatcgtg tataaagaat actttggttc tgcttcagaa
    20881 ttccaaaaag gcgaaaaacg aaatgttgaa ggaaagaaaa tctggatcca acacaaagga
    20941 tctttgaaag atacactagt ggaaatgcaa caagaccttc aatcatctat ttcttatgct
    21001 ggaggaagag atctggaagc cattcgaaaa gttgactatg tcatcgtgaa aaattcgatt
    21061 tttaatggag acacaatcta aaaaataaat aaaatattag gatgttgtga aaaagttgtc
    21121 ctgcattaag taataagaac agccaaacaa aaatagttct ttatatgttt tgtttggctg
    21181 gaattattat cgcagataca agcttttgaa catcattatt cagaataagg gactgtgata
    21241 agatttttgt cacagtccct tatgttttgc aaaaaaagtt tttctttttt ctcgtaaatt
    21301 cggtaaaatc aaaagaaaaa atagacaata gaaaagagaa gcttatgtgg gaaaaagtag
    21361 agaaaaaaca aggaccgatt tatcgtcaaa ttatggaaca gattatgaga gaaatcgaaa
    21421 acggaaggat caatcctgga agtcaacttg aatcagaacg aaaactaggg caactttttg
    21481 gtgtgaatcg ttccaccgtt gtccatgcat tggaagagct tagggagctt ggggtactaa
    21541 cgagcaaacg tggcagcgga tggtttgtta atcagactga atgggggaaa ttcgctgttc
    21601 caagaatcga ttggcggcaa atgatctctc ctcgttatga gcagacggac tggtatgagc
    21661 aaaaaataag agaagcaaag cagatccaaa aaacatcgtt tcttgattta tacgctagcg
    21721 agatgccaga tgagcttttg ccgaattttg agtttccttc ttattctctt gaagaaatct
    21781 tagcggaaga aaaagaaatg gatcttttag gctatactcc gttacgtaac aaaatcaaac
    21841 ggtatttgga aagtaaattt gaattggcat tttcccccaa ccagcttctt gtgactggtg
    21901 gcggtcagca agcaatcttt ttgattttac aaacgattct ttcagctgga gaggggatag
    21961 ctgtagagtc accttctttt ttttaccgat tacctctttt caaagcttca ggaacgcgtt
    22021 tatttgggat accgatggat gaagaaggga tcgacttaga aaaactgaaa gaatcgatcc
    22081 ataaaaataa attgaaagca gtgcttatca acccgaattt tcaaaaccct acaggaaaag
    22141 tgatgagtca aaaacgacga gaaaagctag tagaattgtg tcgacagtat cggcttccaa
    22201 ttattgaaga cgatgtattt acggatcttt catttctaaa ccaagaagaa gtccttccta
    22261 tacgaacgat tgatccggat aatgtgttat acgtcggttc gttatctagg gttttaggaa
    22321 aaacaacgaa aatcggttgg gtgataggac ctgagacttt tatctcgcaa ctagctcaag
    22381 cgcaagaaat gatggagttt tcgatgaata tccttacaca aatcacagtg gctaatgtat
    22441 ttaatgaagc aattgatttg aaaatgcaag atgtaagaaa aaagttgaaa gaaaaaagcc
    22501 gactgataag cgcttgggga gattcacaat cgtttttcaa gcttcattca attttaggtg
    22561 gttactatgc ctggattact tgggatggga aaaaactaga ccccgaacta gcagaaaaag
    22621 tagtagatat gggtcttgga attgcaccaa gtctactttt tgggcaagat ataaatggaa
    22681 tcagaatcaa ttacagtagg ctggatgaaa accaaatacc gttgttttat tcaaagatga
    22741 aaattctaga aaagtggtta acagaataat ttcacaaagt aaatagatta aagaatataa
    22801 cagcttggat ttaaatttca agctgtttct tttttttcgg aaaaagaata cgatataaag
    22861 aaactaagaa aaaaccatta tttgtacgtc cgttcacgat gtgtaaataa aataaatgtt
    22921 tgcaacaaaa ctgtataaca ccaacgtttt taaatagaaa cagatggttg taagtttgat
    22981 tagaaaatag aaaaaatgca gatgaaatat aaaacgcttt ccttattgtg attttattca
    23041 ctaatgctgt taatatgcgg attggcataa tttcctttca tcgtgaaaat ttgtgctttt
    23101 agcttgtatt tctcataaat ccatgataat ttattaatga agacggttaa atagtgatac
    23161 agttttattc actgtttcaa tcgcagaatg aagagatttt cttatagatg agagaggaat
    23221 gtgtgacaaa tggcaaacaa aaaagcaccg atcgacttcc aagcactgct agatgaagtc
    23281 aatgcagatt tcccagtata tcaagcactt gatcaagatg gaaacgtagt taacaaagat
    23341 ttagtacctg atttatccga tgatgaactc gttgaattaa tgactcatat ggtatggtct
    23401 cgtgtattag accaacgttc aacagcgctg aaccgccaag gacgcctagg cttttttgca
    23461 ccaactgctg gacaagaggc aagtcagctg gctagtgctt acgcatttga taaagaagat
    23521 gttcttcttc caggttatcg ggatgtacct caactagtaa aacatggttt acctttatct
    23581 caagcttttt tatggtctcg aggacatgct gcaggtaatt cttatccgga agaattgaaa
    23641 gctctaccac cacaaattat catcggtgca caatatgtac aagcagccgg tgttgcatta
    23701 ggtttgaaga aacgaaacaa gaaaaatgtc gtcttcactt atactggtga tggcggttca
    23761 tcacaaggtg atttttacga agcaatcaat ttcgctggcg cttaccatgc aaatgccgta
    23821 ttttatatcc aaaacaacgg gtatgcgatc tctacacctc gtgaaaaaca aactgcagca
    23881 aaaacacttg cacaaaaagc tgtcgcagct gggattcctg gtattcaagt cgatggaatg
    23941 gatccgcttg cagtatatac tgtatctaaa atggcacggg aatgggcgat cagtggaaat
    24001 ggaccagtcc taatcgaaac actgacttat cgttatggac cacatacact atctggtgac
    24061 gatccaaccc gctaccgtac aaaagacaca gatgacgatt ggcagaaaaa agatccattg
    24121 atccgcttcc gtaaatattt gacggaaaaa ggactttggt ctgaagaaaa agaagaacaa
    24181 gtcatcgaag caactaaaga agaaatcaaa gcagctattg ctgaagccga caaagtacca
    24241 aaacaaaagg tttcagattt cttgaaaaac atgttcgaag tatctccaca atcaatcaaa
    24301 gaacaaattg caatttatga agcgaaggag tcgaaataat catggcacaa aaaacaatga
    24361 tccaagcaat cacagatgcc ttagcacttg aactagaaaa tgacgagaat gtcgttgttt
    24421 ttggtgaaga tgttgggaaa aacggaggag ttttccgggc aactgaagga ttgcaggaaa
    24481 agttcggaga agaccgagta ttcgatactc ctttagctga atctggaatc gcaggattga
    24541 gtttcggtct tgcgttagaa ggatttcgtc cagttcctga aatccaattc tttggtttta
    24601 ttttcgaagc aatggacgaa gtagtagctc aaatggctcg tacaagatat cgaatgagcg
    24661 gcactagaaa tctacccatc acaattcgtt cgccatttgg tggcggtgtt catacaccag
    24721 aattgcattc ggataattta gaaggtttga tcgctcagtc tcctggtatt cgagtagtga
    24781 ttccttccaa cccttatgat gcaaaaggac tattgatttc ttctattaga agtaacgatc
    24841 cggttgtttt cctagaacac atgaaactat atcgctcatt ccgagaagaa gtgccggatg
    24901 aagcttacga agttcctttg gataaagcag cagttactcg tgagggaaca gatgtatcta
    24961 ttatcacata cggagcaatg gtgcgtgaag caatcaaagc agcagacaat cttgcaaaag
    25021 aaaatatatc tgttgaaatc attgatcttc gtacagttgc gccattagat gtagaaacga
    25081 tcattcaatc cgtggaaaaa actggtcgcg tagttgttgt ccaagaagca caacgacaag
    25141 cgggcgttgc cgcgcaagta gtttctgaga tctcagaacg cgctatcctt tcattagaag
    25201 ccccaatcgg acgagtttct gcacctgata cagtcttccc atttggacaa gcagaaaatg
    25261 tctggttgcc aaatgcaaaa gatatcgaag acaaagtaaa agaaatcgcg gaattttaag
    25321 aaaagaaggg acgaaacaaa gtcatggctt atcaatttaa att
//
LOCUS       DACGLW010000040        24578 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.040, whole genome shotgun sequence.
ACCESSION   DACGLW010000040 DACGLW010000000
VERSION     DACGLW010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24578
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.040"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            complement(153..551)
                     /locus_tag="IXT53_001589"
     CDS             complement(153..551)
                     /locus_tag="IXT53_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HAQ4905419.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(548..1321)
                     /locus_tag="IXT53_001590"
     CDS             complement(548..1321)
                     /locus_tag="IXT53_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAQ4905420.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1318..2001)
                     /locus_tag="IXT53_001591"
     CDS             complement(1318..2001)
                     /locus_tag="IXT53_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HAQ4905421.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2168..3055)
                     /locus_tag="IXT53_001592"
     CDS             complement(2168..3055)
                     /locus_tag="IXT53_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ4905422.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3052..3921)
                     /locus_tag="IXT53_001593"
     CDS             complement(3052..3921)
                     /locus_tag="IXT53_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ4905423.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4058..5344)
                     /locus_tag="IXT53_001594"
     CDS             complement(4058..5344)
                     /locus_tag="IXT53_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ4905424.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5617..6195
                     /locus_tag="IXT53_001595"
     CDS             5617..6195
                     /locus_tag="IXT53_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HAQ4905425.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6195..7124
                     /locus_tag="IXT53_001596"
     CDS             6195..7124
                     /locus_tag="IXT53_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ4905426.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7121..8857
                     /locus_tag="IXT53_001597"
     CDS             7121..8857
                     /locus_tag="IXT53_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ4905427.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(8920..9756)
                     /gene="pheA"
                     /locus_tag="IXT53_001598"
     CDS             complement(8920..9756)
                     /gene="pheA"
                     /locus_tag="IXT53_001598"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HAQ4905428.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(9753..10262)
                     /locus_tag="IXT53_001599"
     CDS             complement(9753..10262)
                     /locus_tag="IXT53_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HAQ4905429.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10255..11550)
                     /gene="aroA"
                     /locus_tag="IXT53_001600"
     CDS             complement(10255..11550)
                     /gene="aroA"
                     /locus_tag="IXT53_001600"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HAQ4905430.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11563..12654)
                     /locus_tag="IXT53_001601"
     CDS             complement(11563..12654)
                     /locus_tag="IXT53_001601"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HAQ4905431.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(12671..13837)
                     /gene="aroC"
                     /locus_tag="IXT53_001602"
     CDS             complement(12671..13837)
                     /gene="aroC"
                     /locus_tag="IXT53_001602"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HAQ4905432.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(13839..14903)
                     /gene="aroB"
                     /locus_tag="IXT53_001603"
     CDS             complement(13839..14903)
                     /gene="aroB"
                     /locus_tag="IXT53_001603"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HAQ4905433.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(14936..15958)
                     /gene="aroF"
                     /locus_tag="IXT53_001604"
     CDS             complement(14936..15958)
                     /gene="aroF"
                     /locus_tag="IXT53_001604"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HAQ4905434.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(16617..17591)
                     /locus_tag="IXT53_001605"
     CDS             complement(16617..17591)
                     /locus_tag="IXT53_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HAQ4905435.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(17572..19455)
                     /locus_tag="IXT53_001606"
     CDS             complement(17572..19455)
                     /locus_tag="IXT53_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HAQ4905436.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(19473..21698)
                     /locus_tag="IXT53_001607"
     CDS             complement(19473..21698)
                     /locus_tag="IXT53_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAQ4905437.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            21813..22847
                     /locus_tag="IXT53_001608"
     CDS             21813..22847
                     /locus_tag="IXT53_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ4905438.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(22914..23927)
                     /locus_tag="IXT53_001609"
     CDS             complement(22914..23927)
                     /locus_tag="IXT53_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ4905439.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(24005..24193)
                     /locus_tag="IXT53_001610"
     CDS             complement(24005..24193)
                     /locus_tag="IXT53_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HAQ4905440.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
BASE COUNT         7064 a         5341 c         4397 g         7776 t
ORIGIN      
        1 agcatatttt gaaaagaaag tctgatgctt agagtagaat ttcataaaaa tttactattt
       61 cataagtatt cacgagaaaa atacaaacaa aaaagcggaa ataacgctca tccgccttct
      121 tgtttgtgtt tatttcctct tcaaacgctt gtctatgatt cagacttttc tctatcgtta
      181 ttttcatcta aaagttcttg cattccgata atttctcgat attgatgaat cttagtgata
      241 ccaatgactt tgtctacatc aatactaaaa caaataccat tacctggaat atcgatgttg
      301 atgccttgac caatcgcatc cattacttta ttcgtcaaac tatcgggtac taatgtcaac
      361 acaatatctt tttctggcga aatgtccaaa cctaaaaatt tccctgaatc atgtacacca
      421 gaacctcttc catgtaagat cgttccgcct gctgcacctg cttcttttga aaagtcgata
      481 acatcttcgc ccattcctcc gtcaacgatc gtcacaatca tgtgaagatt caatgctaca
      541 agatctttca tttgctttcc cccaattttt gcaatctttc tttttcgatt ctgtcttttt
      601 catcttttcg tttataaata tatcctagta ctaaagtgga taatattggt gtcaatgcaa
      661 tcattgcaac cacaccaaac ccgtcaatga tcggatctct tccttcaatt tgtgcagcga
      721 ttgccacgct cattgataaa ataaatgtcg aacacatcgg gcccgttgct actccaccgt
      781 tatcaaaagc cattgctaaa aagatcttgt ccacttttcg tcctaaaata aatgctagcc
      841 cgtatcctgg gatcaagaaa taataaagag agaatccgac gagcaaacgc cacatagaca
      901 atcctactgc tgcgccgatc ccaattgaaa ttaccatcag catgattttc gatcggattc
      961 tcccctcgct caattcttca acctgtttga tcattacatg aatcgccggc tcagcaaacc
     1021 ctactaagaa ccccattaaa aagccaaggg gaaccagtat ataattgtaa ggcaatcccg
     1081 caatagcact gcctaaatgc tgtcctaccg gtacataagc catattgact ccgtgcagga
     1141 aaaggatcaa cccaatagtc gttatcaaga aacctttcat aatgtcaaag aaacgctttt
     1201 tgcgcatacg aaaactaatg atattcatta aaacaaatag gattacgata ggtgccactg
     1261 ccaaaatgac ttccatggta acgatttcga ttccatctat cacttgctgc cagaaactca
     1321 ttggaagatc acccccaaaa tcatcacggc aagaatcggc cctaacgaag caataccgac
     1381 cattccaaaa ctgtcatttt catttttctt ttgtttgatc atactagtca tccctccggc
     1441 aagggccaag ataaatggaa cagttactgg accagtagtc actccccccg aatcaaaagc
     1501 aatcggcata aattcggaac tggtgaaaaa tgaggcgatc aaaacgccta tgtagcctat
     1561 agccatcaat tgataatagg atagcttgaa tacgacccga agcagcgcaa aggctagaaa
     1621 tactccagtg ccgacgctga cgatcccaat cagcaacact ctgccaatct ctccgtcaga
     1681 tatatcatat acttgttgtc ccaaaacttg cacggaaggt tcagcaatcg tgatcacgat
     1741 cccgatagca aatcccaaac tgaccaaaac ggtcaaactt ttctttttga tcatatattt
     1801 ccctactaga tctccgactt ccatcataga ataatctgct ccaaacaaaa agagcgtcat
     1861 tccgatcatc atgattgccg caccaaccag aaatgaagcg aggtcttctc cttctaacgg
     1921 tgcaaaaaca aacgttaaaa tgacaatcag gacagtcatc ggcaatatag cagcaatcac
     1981 ttctttaaaa ttttttagca tgtatcctcc tcctttactc tttaagttta ccttaaaatg
     2041 aaagagtttt cgagcacaaa cttaaaacgt tatcatttaa tttttctcaa cataaaataa
     2101 cacacgtccc aaaagtatct ttttacttgg aaaaatgact ttgaaggaac gcgcttattg
     2161 tgttttttta ttttacagag ccgatcattc cttgaacgaa ttgcttttgc aaaacaaaaa
     2221 atacgatggc agttggtaaa gtagcgatga ctaatccagc catgatcatt ccatagtctg
     2281 gtgtatagga agcacccata gcagataaaa caagcggaac cgtccgttta tcaggagatt
     2341 gcaaagctac taagggccat aaataattgt tccagctact catgaatgtg atgattgcag
     2401 ccgcggcata ggtattttta gccgtcggca tatacactct aaaaaaaata cttagttctt
     2461 ttagaccgtc taatcgagcg gcttctacta gatcttttgg aaaagattta acattttgtc
     2521 gaaagaagaa gatcaaaaaa gcagtgctaa cagatggcag gatgacaacc aagaaactgt
     2581 tgattcccaa aaccgttccg ttcaattgtg aaaacattcg atataaaggg atcatcaatg
     2641 ccgcaaaagg aaccatcatc gatagcagta gaatattgaa aattaggtct tttcgtttac
     2701 tttgataaat ctcaaatcca tatcctgcca ttgacgaaat cagcaatgca aacacagtgg
     2761 tcaccaaagc gatcatcgct gaattccaaa gggaacgagt aaaattcagc tcattggaaa
     2821 acaaattttg aaaattgatg atcaagttac tgccaatctt tatttttccc gatgtgatat
     2881 ccacgggtgt attcgtcatt cccaaaacca tccagacaaa agggaaaatg gaaataaatg
     2941 ataggatgct taatgctgta tactttaata ctgacccaat tgtcgaacga agttctaatg
     3001 cttgtttttt gacgttgggg cgttcgattg tttctttttc taccatattt ttcatcgact
     3061 ttctcctcct actttcgttt ggattatcga caatatcaca ataaaggaaa caataacaaa
     3121 ggaaactgct gccgcatatc caaaattagg cgtgaattta taactcaaat tatagatgta
     3181 ttgagatagg gtggtcgttg catttgctgg tcccccgcca gtaatatttt gtacttcatc
     3241 aaaaagctgc aatgtcccga tcgtcgaagt aatggaagta aacaaaatga ttggttttaa
     3301 atttgggatc gtcactttga taaactgctg ccatttagaa gcaccgtcaa tagatgctgc
     3361 ttcataaatc tccgaatcaa tattttgcat gcctgaaagg aaaaaaatca tattgtatcc
     3421 agtccaacgc caagtgatcg aaagaatgat cagaactctc gcccaaattg gatgagtcaa
     3481 ccagccaatc ggtgtgtcaa tcataccaat gcccaacaac aagttgttta caaggccaga
     3541 ttgagaaaat agacttttca tgatcagaga ataactgacc atcgaagtga tacatggaag
     3601 aaagatagcg gttcgaaaca accctttata tttcaacttt ttatcattta aaaggttgga
     3661 aataaaaagt gctagaaaca acatgatcgg tacttgtaca agtaaataca ggaatgtatt
     3721 aaaaaaagct tttttaaaag ttgcatctgt caacatccgc tgataattac ctaacccgtt
     3781 aaatgtcata ttgacagggg gaccactttg aaaagacgtg atcaaagcca aaaccatcgg
     3841 aataaaaacc atgattgata tcaaaatgac tggcaagatt aaaaaagcat gcccgttttt
     3901 gaataaatct ttctgcttca ttcttttccc tccaattcta tcattgatta tctacatgaa
     3961 aagacaagaa agacaatatc tgtaaggcag acgtgatctt ttttgtcttt tcgtttgtct
     4021 gatgaaaaag cgaagaaatt cactttttta ccaccgatca attagccaat tgtgcttcta
     4081 cttgtttttg tgtatctgct agtaccttgt ctgtctcttc tccattgatg attcgatgaa
     4141 gagcttcagc tacgactgct tcaattgcat aggtctcatg tccgtaattc acttctggta
     4201 tttcagatgt ccatgttgaa aaatcgtcaa agatcttttg accactgtaa aattcagaag
     4261 cttcttgata agcttcttgt tcattcgcgc taagcatcgt agatactagt ccgatttctt
     4321 tcgcaagagt gcccatcagt tcttcattcg aagcaaacgt tgcttctagg aaatctttcg
     4381 ctgaatcttc tccagctact cctttgatca cataccagcc agcgccgcca aggttagaag
     4441 cttgcgcttt ttgcagattt tctggaagtg caggtattgg tgcaatcttc cattttccag
     4501 attggttttc tgctccttgg atcgttgaag aataccaagc gccagttggg ctagaagcta
     4561 ctgctcctga ttgtatcgca gttactccag cattccagtc agaggtttgt tcgactagat
     4621 cttctttcaa aagagtggca aacaattcaa gaccatactt taaagatgca ttgtcttgga
     4681 tcgtgactgt ctccccatct tcagatgtat accattctcc tgcctgttgc ataatcatcc
     4741 ttactcgacc aaggtcagaa ggattgactt cagcgatttt ctttcccgtc ttttctttga
     4801 catctcgcgc tacttgaata tactcgtccc acttcagatt ctccatatcc tcttctgtat
     4861 aacctgcttc tgccatcaga tccgtacgat agaagtttgc tgttacgccc gaatcgaaag
     4921 gaacaccgta tattttatct cctactttgt ttaccgcaaa tttatacggt gcaaaatgat
     4981 cttcctttac aatagcagac aaattagcga atgcatctgg ataagaagtc aaatagcctt
     5041 gtatcctata atcttcaatc aagacgatat tgggcaatcc ttctgtattc ccgctcgcta
     5101 aagcggtgtt cagtttctga acgatatcat cctgagacat cgtaacaact tctatctctg
     5161 tttcttcgtt ctcataaact ttttttgctt cattcactgc tttgatattg aatgtctcat
     5221 cccacgccca gacagtgact ttttcttctt tgtctgctgt actactagca cttccgcatc
     5281 ctgttaagaa acctgatgct gccgctaaac aaacgactcc agcaattaac tttctgatgt
     5341 tcatttttat ccgctccttt tccatccatt agttgtttac gtttgctaca ttcatcatac
     5401 caaaatttga aaacggtttc tttattattc agatacttca aaaatttctc ctaaaataat
     5461 aaaaaaatac aaatcaacta tgacaaaagg aataatctta gcatctagac caaagatcga
     5521 aatggtgagg tttctttaaa ataaaaatat ccttgcattt tctcctagaa ataagtattt
     5581 ttaaattaca agactattct taaggcgggt aaaaaaatgc caatacgcgt aattcttatc
     5641 gatgatgaac agacgatttt agatggaatg aaacagttga tcaattggtc tgagcacggg
     5701 tttcaattaa tagggacttt ttccagtccg ctgaaagcgc ttgatttttc tttggtacat
     5761 gcaatcgata tcgtggtcac agatgtaatg atgcctgaat taaatggaat cgaattatcg
     5821 cggctattaa aacagcaaca acctgaaaca cagatcctta tcctctctag ctatgatgaa
     5881 tttgatatgg ttaaagattc ttttaaagaa ggcgtcgctg attatctttt aaagcctcga
     5941 ttgagtcctg atttttttct aaattcctta actgccgttt cacaaaaaat caaaaaaaag
     6001 cccaacaaaa gcccaatcac caatcgttat gagcagatct gcgagaatct ttcacagcag
     6061 attgctggac aaactcgtcc attgcttgac cctcttcttt ttgaacacac cttgtttttc
     6121 tgttatatac cgaaaaacgg ccattcagtt caaaacaagg ctctaaacaa aaacagtcga
     6181 tcacccattc attgatggat agcgacgatt cgtttactat ttatcctgtt tctacctttt
     6241 cggaagaatc cggctatgta atcaatactg attccaaaga tacacttagg aagtttgttc
     6301 aaaagtttcg tcaagaagtt tttccaggtg atttttttgt tgtttctgat tcttttgcaa
     6361 tccaagatat caatgaaata ttccaagaga tcagaaattg caccaaagga caacgtttct
     6421 atcataaggg aaagaacctt gcttatcagc atgaacttct tccgttatta accaaccacg
     6481 agcagcaagc aagtagttat ttaacaaaaa tcgttcataa agactacgct ttagctgtca
     6541 aagaacttga gaatttcttc acgacgatat tggaacagcc tattgatcca gcttatttga
     6601 aacatgaggc aatcaatcgt ttctatgcat tactcaacgc tttggctgac gagcatgttg
     6661 ggaatgaaga actttctttg ctcaagatcg cccttccttc tttattagcc gatgctgagt
     6721 atttcgaaga tttttctatt cttgtcaaag aaacacttga gaaactcacc cacttttctg
     6781 caaatgcaaa aaaagacaat ttcgatatct tgactttgat ctacgagttc gtccaggaaa
     6841 attatgagaa agaaatcaat ttacaacttt tatccgaaaa atatcatttt tcatatagct
     6901 atctctcagc catctttact gaaaaatttg gtatcaactt ttcaaaatat ctaaaaaaag
     6961 tccgtatcag taaagccaag ctctttttga cggaaacacg gcttactttg acggaaatct
     7021 gtgagaaagt cggctatacc gaactgggtt atttctccag agtttttaaa gaagagacag
     7081 gaatgacacc ttctcaatac cgtaaaggaa cttttgtaaa atgagaaaaa aaatcaaaga
     7141 gcatgaactc tttgccaaac ttctgttcat tttgttcatc ggtctttttc tgcaagccat
     7201 cgtcatttct ttgtttattt atcatcgatc aagggatgcc tatatccagc tgttcaatca
     7261 atcgaatgat gtggtactaa aaaagattca atctgaattt gaaagtttga atgatacaat
     7321 cgagaataca ttagccgctt ttgattctaa tccagctgtc aaaagctatt tttccaatga
     7381 tccagcccaa catatggaac agcttcagca actaagaaca atccaaaaaa tgaatgactc
     7441 tctttcaaaa atccatccaa tgatcgatta cgatgtattg atctttggag aaaatggaag
     7501 aacttttgtc ggaaacgaca tgctcactgc cgtatctgcc gattcttttt ttcaatctgc
     7561 gattgctcag agagtgaatg aacgtgctgc tgatacacaa atgctttttg cacaccacgg
     7621 attgactctt cgcgataaga aagcacccag cgtcttcttt gtcaaaaaac tgaaaaatac
     7681 attgaatcac gtttacggat atgctgtttt atcgatctca tccaagcaac tagcgaatct
     7741 ttttcaatct gtagtaaatc ctgagatatc gaagatctcc ttcatgaacg aagaacagac
     7801 gatcatcgca tcaaatgaag aaaatacaat tgggagccga tcaatgattt tcaaaaaact
     7861 aacagtaggt gaaacaacga tttcaaatgg acagcagttg acccgtcttc ctctgtaccg
     7921 ccaggattgt acattactca gtcaagtcga tcttcattct ttatctaacc aaatgggcgt
     7981 gatttttccg attattttgt ataatatcgt gacacttgct tttgctggct cattggtatt
     8041 tatgtatttg gatcgacaca caaaatcgat ctaccgcttg attgattcgc taaaacggct
     8101 agaggaagat ggcttaaaag ctactgttcc tgtacaagga agccatgaag tccaattgct
     8161 agggaatacg atcaatcaat tgctaagtaa tgctcagcat cattatgaac agacgatcca
     8221 agacgaaaag aagaaacgtt cgcttgaaat ccaaactatg caagcacaga tacagcccca
     8281 ttttatttat aatacgctga ctagcttgaa atttttgatt tggcagaaaa aaacagatga
     8341 agcagttcaa ggaatcgaca gtttcattac tttactaaga agtacgattg ggaaaaaaga
     8401 agaagtgatt ccagtcaagg aagaaatcaa aagcgtacaa agttacatcg atcttttgag
     8461 tatgcgatat ggcgaaagaa tctcggctaa aataatgatt cctgatgaac tgcagtcatt
     8521 atctatgccg aatatgatcc ttcaaccaat tatcgaaaac gcctatcttc atgcttttca
     8581 caataaaacg caaggattca tcacggtgta cggcaaaaca aaaaatcagc ttcttttatt
     8641 cgaagtgatt gataacggcg atggcttcga tactgaaaat accaaacaaa aaaatgatca
     8701 tttttcagga atcggcatca gccatgtaga tgagcggata cgcctaatgt atggcgatgc
     8761 atatggctta acgatccaat cagtgattgg gacaggaacg actgtaacga tttgtctacc
     8821 gcttcttcct aaaaatcaat cagaatatca aaaataaaga atagccaagc gctgattttt
     8881 ttattttaag ttaaataaat caatcagtat tcttgacttt catctacgcg tctcttctac
     8941 tgtcagcact ggataacagc ccaatatttg actttctcct ccgagcatct tgatttcttg
     9001 gagcgcattc tctactaatg tcattggacg gtccaacaac aaatcgatca cgaaaaaata
     9061 ttctcccaag ctagttttca atggtcggga ttcaatctta cttaagttga tttcccgcca
     9121 gccaaaagct gccagcattt tatgaagcat ccctggccga ttagcaggta gtgtcaagat
     9181 gacactcatt tttactggag ctgactgctg gctggtcatc tttcggtcac cgacgatcca
     9241 aaaacgtgtc tggttcaact cattatcttg gatattctca gctaagatct ccaatccaac
     9301 atgctgtgct gtttctaatg aagcaatcgc tgctgcgtct tctttcggat gttctgctac
     9361 atacattgct gcagcagttg ttgattctgt tgctactaaa ggaacattag gataatgcgt
     9421 ttctaaaaac tgttgtgatt gtgctagtgc ttgtggatgt gaaaggattt ttgtaatctt
     9481 tgttgctgga ttgcctaaaa gctgctgctt gataggaaga acgatttcac ttttaacttc
     9541 gacttctgga tgtttggaaa gaagatcgat tgtgtgatga acagctcctt ccaatgaatt
     9601 ctctacagga acgacagcca gatcgacttg tttgcgaaac aaagcgcgca aacaaacagg
     9661 gatggaagca tacgcagtca attgttctgt aggaaatagc tgacaagccg cctgataagt
     9721 gaatgaactt tctggcccta gatagctgac gttcatatgc ttttgatcct ttctataatg
     9781 gtatcgatga tcttttctgg tgtttgattg gtcgtgttga ttcgatgtgt cgcactttct
     9841 tcataccaag aaagtcgttg tgccaataat gcttttattt cttctcttgt ttttccaaga
     9901 gctaaaggtc gaatagacgt ttcatcttgt tggatacgat caactagaca gtccgcttct
     9961 gcctctaagt aaagaacaat cggctgtgtt tttaaaaaac gccgattttc tgcactttgg
    10021 acaatccctc caccagtcgc gataattttg tcttcatttg acagctttct taaaatatct
    10081 gactccactt ttcgaaagct tttttcaccg taatagtgaa aatattcggc aatcgacaat
    10141 tggatatgtt cctcaatctt tgtatctaaa tctacatact ctttttgtaa acattgagct
    10201 aaccctttac cgattgttgt ttttccagcg cccatgaaac caatcagcaa aatgctagcc
    10261 atgagcttct cctcctgaca caagttctgc taaatcgtca aaaaatgccg gataagagat
    10321 tgaaacagct tcagctcctt ctagttcgca aggtccatca gctatcaaag cagctacttg
    10381 cagcatcata ccgattcgat gatcgcctct gctggacact tttgcgccat gcagaggtgt
    10441 cggcccatgg atgatcagac cgtctgctgt cgcttttatc tttgctccca ttttctgtaa
    10501 ttcttctgcc accgcatcaa ttctgttggt ttcttttact tttaattctt ctgcgtcttt
    10561 gatgatcgtg attccttctg cttgagtagc cactaaagct agaatgggca attcatcaat
    10621 cagacgcgga atgatttctc caccaatcgt tgctttcttc aaatgactcg tcttgacact
    10681 taaatcagcc gattggttct gttcattgtg attcgtctgg gtaatcgctg cgcccatctc
    10741 ctccaacaca tctaagatac cagttcttgt tggattgacc ccgacatttt ttaacaacag
    10801 ttggctttcc ggaacaagaa gtcctgctgc tagaaagaaa gcagctgatg aaatatcacc
    10861 tggaacagtt atctgttgac cagttaattt ttgcggtccg gtcactatga tcgttttatc
    10921 ttccactgtg atccttccgc caaattgacg aatcatttct tccgtatggt tacgggaacg
    10981 ttctttttca acgattacgg atgtgccttc tgcttgtaat gccgcaaaca gcaaagcaga
    11041 tttgacttgc gcactggcta caggcatatt gtatcggata ggtgataaag agcgggttcc
    11101 tgtcacagtg attggtagat actggtcatt ttcttctcct tgcaagtcag cgcccatctc
    11161 gcgtaaaggt tccatcactc gccccattgg acgtttagac aacgaagcat ccccgaacag
    11221 agtagtcgta aaaggctgtc cagccaaaat acccatcaat aaacgtatgg tcgtaccgga
    11281 gtttcccatg tcaattgatt cggttggttg gacaaatgag tggctaccgc gaccatgaat
    11341 cttgatcacc tcttcctctt cttcgatctc gacacctaat tgttggaagg catgtaaagt
    11401 actcagacaa tcttctgcgc gcaaaaaatt ttgtatagtc gtcgttcctt cagcaattgc
    11461 tccaaacatg atgctgcgat gggaaattga tttatctgct ggtatcctaa cagtccctct
    11521 taatccatgt atttgttgta ataattgcat ttatccagcc tcctatttga tccagcaatg
    11581 ataactggta ttagacataa tgattctttt tgcttgtaac aaatcctgtt ggtttttaaa
    11641 actgatttgc agtattccta aaatatcttc cctggtctct tggatcttca aattaatcaa
    11701 agaaactccc gcttttccga tcagtccagt tacttctgca ataacgccag gttcatctgg
    11761 cacatcgata aataaatcaa aaaaggcagg aatcgcgcct tgcttatgga caggcaactg
    11821 gtctcgactt tcttttgccc gattgaaaaa agaaaagatt tccgattggt tctcagtcat
    11881 gatccattct gctatttgtg tcatttcttg ttgccaatct gccaatcgtt ccaataatat
    11941 ctgcttattg ctcaacaaaa tatctgtcca catgacagga tctgaggaag cgatcctagt
    12001 gatatctcga aagcctcctg ctgccagttg tttggctcgc ggatactctt gattgaattg
    12061 ttctgcctga ttgactaagc cagctgccag aatatgtgga aaatgactta gcattcctgt
    12121 tatacggtca tgttctcttg ctgtaagagt tacatatttt gctttggttc cgcaaaatag
    12181 ttcttgcagt ttttcaacat cttttttcgt cttttccaaa ggtgtaaaga tgtaataggc
    12241 attttcaaat agtttagcat ctgctgcctg tatacctgat ttatgagaac cagccattgg
    12301 atgtccaccg acaaaccgaa gatttttctg ctcagcggat gtcacgactt gctgcttcgt
    12361 actgctgaca tccgtaatga tgacgtgatc agacaagttg attctttcaa ggacatcaag
    12421 ataagctaag gttgtacgga cgggaacggc taataagata acgtctgctt gttctgcacc
    12481 tgtttcaaaa tcacttgcaa cgtaatcaac gatctgctgt tctactgctt gctcacgggt
    12541 ttttgactga ctatcccaac caactactat cagttctgga ttggtctgac gaatacaaag
    12601 tgccatagaa gcaccaatca gtccaagccc cacgataaaa acatgttgtg ccatcttatt
    12661 tgcccccttt ttagtaatgt ttcaagtagt cacggtactc cttcactgca gctttcaact
    12721 caacaagcga atcacttggg aattttgaca atagctctgt cgctaccaca gtggctacaa
    12781 cattttccgc aacgacgcta gctgcaggaa ctgctgtact atccgaacgt tctacacttg
    12841 ctttgtaagg ttcttttgta tcgatatcaa cactttgaag tggcttgtac aatgtaggaa
    12901 tgggtttcat gaccccgcga atgacaagtg gttcgccatt ggtcattccg ccttcgaagc
    12961 cgcctagatt attggtacgt cggaaatagc ctctttcttc ttcccatagg atctcatcca
    13021 tcacttctga gcctggtaat gaaccagaag tgaatccgac cccaaattct acgcctttaa
    13081 aggcattgat actaaccaca gcttgtgcga ttttggcatc tagcttcgtg tcccattgta
    13141 cgtagcttcc taatccagct ggaacgcctc caacgagcac ttctacaatt cctccgatcg
    13201 tatctccatt tttctttgtc tgatcgatca aatcttttat tctttgctca actgctggat
    13261 caacaactcg tacttctgac gcttcagaaa tttctttgat ttcttgaatc gagatatttc
    13321 caggtatttc tgcttggatt ccaccaagac aaatcacgtg tcctgctacg atgatgtcaa
    13381 gttcttccag aattttcttg gcaattgcgc cgattgctac acgcatggtc gtctctcttg
    13441 cagaggaccg ttcaagcaca ttccgaagat ctcgatgatg gtatttcata ccgcctacca
    13501 aatccgcgtg tcctggacgc ggacgtgcaa ctcgccgcag tgattggtct ttttcaggca
    13561 cttctgctac agacatgaca gttgtccaat ttttccaatc tttattttct accactaaag
    13621 tgacaggtga accaattgtt ttgccgtgac ggatgccaga cgtgatccga acacgatctt
    13681 tttcgatctt catccttcct cctctgccgt aaccagcttg tctgcgagcg agttcgtgat
    13741 tgatttcttc ttctgacaga ggtaatcctg ctggtagtcc ttcaataatg gctgttaatt
    13801 ctggtccatg ggattctccc gcagtcatat aacgcatctt acagtcctcc taagtaatct
    13861 ttgaatgtct ctgtgggaac aggaacgatt tttgcttgcc caatcttttc tagcaagatg
    13921 atattcaatt ggcttcctct tgctttctta tcatgggtta tcgcgtggaa taattcatct
    13981 ggatgactta gatcgtacgt aatcggcaga tggaattttt gaagtatcct ttctaaagca
    14041 gctgtcgatc cagttggaga caagccgatt ttttctgcat gagaagtgat ggctaccatt
    14101 ccaatagcta ctccttctcc atgactgact accccatatc ctgctgtatt ttcaattgca
    14161 tggccaattg tatgaccaaa atttaaaata agtcgatttc cttggtcaaa gacatcttct
    14221 tccactactt ttcgtttgat ttccaacgta gcagtaatga ttggaacagc atgttccaga
    14281 agatcttttt cgtctttcag ctcgttcagc tgtttccaaa gcgttgcatc tgctatagct
    14341 gctgatttga tgatttcagc gattccttct cgtaaacgac gtaccggcaa agtttttaga
    14401 acctcaggat caatcaatac accagctggc tggctaaatg ttccgacaag gtttttcgct
    14461 ttgtttgtat tgactgctgt cttcccgcca atactagaat ctacttgtgc aagtagcgta
    14521 gtcggaattt gaacgaaatg gatcccacgc atgaacgtcg aggccacgaa tgctgctaga
    14581 tcacccacca ttcctccacc aagtgcaagt atcccatcgc tgcgggtaaa atcattatct
    14641 gctaaaaaat cgtataactc agctgcttgt tctaaagatt tactggtctc acccgccggt
    14701 acgattgaaa ggactggttc aaaattagct tgtctcaatt ggaacatgat tttttctgca
    14761 tatagtggag caacgtttgt atcagtgatc acagctactt tttgaggtct ccaaatcgtt
    14821 tgaagccatg atccagcctg ttctagtgcg cctttttcaa tatgaatatc gtatttttgg
    14881 ttatcaacat gtacagtaag cattcgtttc acttctttct tttaaaaagc tggaattaat
    14941 cgagtgtctg aatcttttcc attgcttctt tcatgatcgc aacttctttc atcatgcgtg
    15001 agtaagtctt ttcattcaac gattggggac cgtctgacca agcatttgca ggatcaggat
    15061 ggatctctac gatcatgcca tctgctccgc ttgccacacc cgcacgagcc attggtggta
    15121 caagttccca aattcctgta ccatgacttg gatcaacaat cacagggaaa tgagttaatt
    15181 ttttcatcaa tgggacagca cttaaatcaa acgtattacg tgtcgctgtt tcgtaagtcc
    15241 gaatcccacg ttcgataaaa atgacaggag atttgtcttc gactgcaatg tattctgcag
    15301 cattaagcca ttcattgatc gtaccggaaa ttccacgttt caatccaaca ggtttacctg
    15361 ttttaccgac agcagatagc agtttaaaat tttgcatatt acgtgcgcca atctgtagga
    15421 tatcgctgta ctcagccacc atatcgatat gcccttcatc catcacttca gtaatcactt
    15481 tcattccaaa ttcatcagcc gcttgacgga tgtatttcaa tccttcttct tctaaacctt
    15541 ggaaagcata tggagaagta cgtggtttga aggcaccacc tcgcagaatt tttgcgcccc
    15601 cggctttggc cattcgcgca cattctctga tttggtctaa tccttcgata gaacaaggac
    15661 cagccatcgt cacgaaactg ccgtcaccaa tttttactcc gtctacatcc acgactgtat
    15721 tgccaggatg gaattctcga gaagtcaatt tgtatgtgtt taaaatacgc acagcatttt
    15781 ctactccttc atagcgtgta aatgctcctt cttgtaaact tcgcgtatca ccttttagtc
    15841 caatcactac tcgttctgtt ccttgatcga ttgagacttt gaatccttct ttttcgatat
    15901 gatcgacgac tttttttatt tgttcttccg ttgcttcttt tttcataatt actatcattg
    15961 tttttcccgc tttcttttgt ttacttatct atcttggctg attttctcta ctttttcaag
    16021 gtggttttac attaattcct actaaatgac cgtgtcctgt tgttctcatt ttcgctcctt
    16081 ctttcctact tatgacccag ataatcaaca aaaaaagatg tccctgtagg aaattgcctc
    16141 tacagtgaac atcttcaacg agccttaaaa agcgctgcaa aaatataaaa tagccactat
    16201 agatgaattt ctctacgtgg ctgtaagtcg tgtttaaaaa tctttagcca tcatagatac
    16261 aaacctggtt tgtttgtacc cacgaagtca tgaatacaaa cctatctaaa atagctaaag
    16321 taatacgtat tcagttttgt aagttttcta gatttcataa aaaatcgctc caaactgatg
    16381 taagaattgt acctagtata gacataaaaa aatcgactgt caatcatttt tatgaaactt
    16441 tttttaattt tctgatagtt aactttgttt ttcagaaaaa taacaattgg ttttattgaa
    16501 gaaaatagtc atgaacaaaa gtatctgaga acttttttac tctcttgttc atgacatata
    16561 gaataaagca gctttgtctt gtaaactccg tttttcaaat aataatctaa agattgctac
    16621 tgaataataa agccgatttc atggtcctct tcgctgctga ttcggtaagg tgactcaaca
    16681 tccgctcccc agctattgat acccccgact cctcttactt ttgcaaaaat cgtaagaacc
    16741 gttcgtcttt ctggcggtag ttcttctata tgtgttgcct gttccagttc ttctgctttg
    16801 aatggtaaag cggaaaaagc aaatggcgta tcctgctgtt caaatcgcaa cgaaaaatct
    16861 tcttgatctt tatccgtatt atccaacgtt tgatttcttg ttagttctac ccatttcgtt
    16921 tccatatgca tgccgcattc ttggggaaca aggtatgggg aaacggggag tcctgccact
    16981 ttataaatac cttcgacacc accagccatc cgatccggat atgtttcccc tgataggcct
    17041 tcatactcaa aaaattttgc tttcgttggc ataatgaacc ttaaacccaa tgcgggtaat
    17101 tccggtaatc ctttttgtcc ataataatgc atattgacaa gcattttacc ctctccagcg
    17161 atttggtagg aaattgtcac ttgggttttt ggtactgtgt tgatttccag tgtaaaaatg
    17221 atttcaaccg tttcagcgta ttcattactt gaatattgat tatttactgg agcaatcggc
    17281 aaatcgatct ttttattgtc caccagtact tctatttctt tgacttttgg atacaatccg
    17341 gctcctaacc acataccgga tttaaatcca aatccgctac ctctgtcatt atcggtcgtt
    17401 gctcgccaaa aaactggaag tgtttcccgg tatagccatt cttttccgtt tttattgagc
    17461 gattccagtc cgcctcttgt ataagaaaaa atataatgcg tattccccga tttgacacct
    17521 aatgtacagt cgccgaagac gattgcaact ttttccgtat ccatatgact tttatttact
    17581 gtatttttca taataatatc ttacctcctg catggcgggt ttttcttgtc catctgcaaa
    17641 aacgatccca ttccctgaga attcatagtc acttggttta tcgtcaaagt caccgccata
    17701 gcgcatcact tctctgcctg tgattggatc agtcacttgg atcgcctgat ctttaaaatc
    17761 ccatatatag cctccttggt acatttcata acgatcaagc aacgtcatgt aagaatccat
    17821 tccacctaac gaattcccca tgtcatgcat atattcacat aagatgaacg gtttttccgc
    17881 tttattttca aggtagtcaa tgatctcttc tggtggcgca tacatgcagc tttttacatc
    17941 tgaaatcgaa tcttcgaatg ctggatttgg aaaattccct tcataatgga caagtcgatc
    18001 ggggtctact tttttgaagt aatcgttcat ttgtcgaatg ttttcttccg cataagattc
    18061 atttccaaga gaccaaaaaa gaatacttgg atggtttttt aatgtttcga aattcgtttt
    18121 tgctcgatca agaacagccg cctgccactg aggtactgca cctggtacgt tgtaagaagg
    18181 ctcaaatgcg cccatttttt gccatgagcc atgggattcg aggttcgttt ccgccatcac
    18241 atggattcct gcttggtcgc ataaataata ccaagtgatc tggttggggt aatgagatgt
    18301 ccgtacgccg ttgatctttg cattttttat gatagccaaa tctttttcca tatcttctat
    18361 agtgatggcg cgtccttttt tcggatgcca ctcatgtcga tttaccccgt tgataatcat
    18421 ccgttttcca ttgaagtaga tgattttatc tttgatttcg attttacgaa aaccgaagga
    18481 atagtcaata tactccgtta ctcttttttc ttcgtctatc gtctccaatt caagcttata
    18541 taaattgggc tgaaaatgcc cccacagtgc cacattttca aatccatcga tgcgaatcgt
    18601 catttgctct tttaacggat attgctgttg atagatttcc ttcccatcag gatctagtat
    18661 cgctacattc agctgactgt ttttggctac ttgctcaaaa gacaaatcta cttcaagttt
    18721 gccattttga catttgtcat ctaagcgagg tttgatccaa agatcttcta aatggttttg
    18781 tcctcgagca actaagaaga catctctgaa gatcccaaaa aatcggaaaa agtcctgatc
    18841 ttccaagaat gcagctgtac ttcttttgta aacctccaca gctagaatat tttcttcttc
    18901 caaataaggt gttaaatcaa attcagacgg agtgaacgaa tcttcggcat acccgataaa
    18961 atgaccattc aaccaaataa acattgcttg ttctacacca gcaaaatgaa tcgagatttg
    19021 ttttcccttt agaccttctt ctaatcgaaa agtcgtcaaa tacgaaccaa ccggattgtc
    19081 tttagcagct gaaaagatcc cttccgcctc tttctcaggg cttgtcgtga aagggggacg
    19141 gcgaaataca tgtccctccc acggatacat cgtattgatg taatggatct tgtcataatt
    19201 tgctaattca atatgactag gaacagtgat ttcatcaaaa gaacaggcat caaaatcttt
    19261 tttataaaat gtttttggtc gtaccgaagg atttggcgaa taacaaaaac gccatgatcc
    19321 atttaaagac tgtttaaggg aggatgtggt ttctacttct tcccttgttg cataacaaac
    19381 atgatcgcta tgtgcatttg attggttcac tctaaaaact tgtggatcat ctaaccattt
    19441 tatatttgct ttcatacttt tttccttctc tctcataact ttttgaaatg aagcagttct
    19501 gtttggaaat caccttgaaa atcggggaag taaagtccaa ctgtcatcaa ttcggccccc
    19561 gaaaatatcc gcttcgaggt ctgttcctga taaagtgcct catcatctaa ccccatcaga
    19621 tatacttcat ggaacgctgg ttgagctgaa gctagtatcc tccctaaaaa cacaatagct
    19681 tcatcttgtt taggagacac aaacatccat gcggcaatat tttcttcaaa aggactcgcc
    19741 aaacgataat aatggccata ctggatcacc ggacgaatcg tttgatagga tataatttgg
    19801 tcttttactt gctttttttc ttcaggagac agctttgtca aatccagttc ataaccaaaa
    19861 acagcgctca tagctgtttc tcctcttgtt ttcaacgaag tagatctgtc ggtctgatga
    19921 tttggtacag ctgatacatg agctgtgatg gacgaaatcg gataagcaag gcttgttcca
    19981 tattggattt tcatacgctc cactgcatcc gtgttatcac ttgtccatga ctgcggcata
    20041 taataaataa acccggcatc aaaccgtcca cctccaccag aacagccttc ccataagatt
    20101 tccggataat cagttgtcaa atgttcaagt agttcataaa gcccgagcat ataacgatgc
    20161 gctacttctc cttgtctctg tgggtcaagg gtaatcgaat agacatctga aagcgaacgg
    20221 ttcatatccc atttgatgta gtcaagagga atcgtatcta aaatctttct catttgtttc
    20281 tcaatcgtct gccgaacatc taaccttgta aaatccagta catgttgtga tctggaagca
    20341 gaaggcattc tacctggctc ctgcataaga aaatcaggat gtgttcgata cagttcagaa
    20401 tcaaccgaga tcatttcagg ttcaaaccat aaaccaaatt tcaatccttt gctatgagca
    20461 taatctgcta tttcacgtaa tccatttgtc aacttgcctt catactcgaa ccagtctcct
    20521 aatgaagaat tgtccgcatc tctttttcca aaccagccat catctaatac gaacatttcg
    20581 atgcctagct cctttgcttc atcaatgatc gccttgattt tttcactatt gaagtcaaag
    20641 taagtggctt cccagttatt gaccagaatc ggccgttcag caaattgatg acgacctctt
    20701 gcgattcttt cacgtagcaa ctcatggtgg atctgactca ttttgttcaa tccctgactt
    20761 gagtaagaaa ggatcatttc aggtgtttga aaagactggc ctgcattcaa ttcccacgaa
    20821 aaacgatggc tgttgatccc accgacgact ctcaattgat tgatctgatc tttttcaagt
    20881 tcgaaagaat ggttgccgga ataaacaaat tgaagtccga tcgcttctcc agtattttct
    20941 gtcgtatgat gatgaactaa agcaataaag ttattcatat ggtgactgct cgttcctcga
    21001 cggctttcaa atacttttgt tccagataaa acagattgcc gtgaaatctg tctttcattg
    21061 acatgtgctc ctggcaaagc aatgacttca tcaaatcgcc tagtatgggc aaagtctagc
    21121 tggaaactag ccgctttttc tagtttgatt gtttctccgg tttcatttct tatctttaca
    21181 gatcgtgtga tcactgctct atcagtaaat atggtataga acaaatcgaa ataaatcgct
    21241 gtttctctat ccttaagtga aatcaccaac gtttgtgcct ctgacttgtc catcacataa
    21301 gtttgaggca agccttcgat ctctggcttc ccatctgtaa tataatggct gtcatatctc
    21361 agatccagta agtctgatcc attcttgcgc tgaatgatcg atgcgggcgt tcgaaagtcc
    21421 atgctgccat gagaagagta ttcttgcggt agtgtatctt tcgacagaga ccggtcagga
    21481 tttaagggaa catttcctga aaatccacga tctcttcggg gataattttt attgtctttg
    21541 tactgcttga ctggtttgcc aaaataaata tgcgccaata cattgctttc ttctatcttt
    21601 atcagataag aaagaaatgt atttcgtaaa tggaagactc ggctcttttc atcaaataag
    21661 atgaaatttt tatctgtttt tctatgcgta atatccatca tttcactccc aacaaaacgg
    21721 aaaccgttta ctttttttta taattcctgt atactgttta ctaaacaatt ttgtcaataa
    21781 aaatttttta tttttttgag gagtgtgtaa aaatggttgg aattagagat gtcgcacgaa
    21841 aggcaaatgt ctctcctgct acagtttccc gtgtgttgaa taacgatcag acgatccatg
    21901 tggctcagac tactcggcaa cggatagtag aggcagctgc tgcgttggat tatgacatca
    21961 gtactcgtaa atatatcaaa aagagaatgc cttcgattgg tgtgatctct acgatcggca
    22021 aagcgagcga agaacaggat atctattaca aacatttgcg agtagggatg gaagaagaag
    22081 ctcgacgtct gcatttaggg atgaatcgaa tctataatct atctgataat cctaaacaat
    22141 gggaagatct agatcagtta ggtgcggtga tcgttgttgg gactgttacg aaagaatcga
    22201 ttaccgcctt gttaaaacaa aacatgcaaa tagtcgtcgt ggataaccct gacatccagc
    22261 aagaggtcga tatggtgtat gcagatcttg aacggatgac taaaaccgta ctggaattgt
    22321 tcttagaaaa tggtcataaa agaatagcct atatcggggg atataaagtc gatatggatg
    22381 aattcggtgg gaagcagatt tctataaatg aaaagaggat gcgtgcctat cagcatttta
    22441 tgatcgatca tcacttagaa aaatatatgt cttatcatct aggtggctgg acagaaaaac
    22501 aaggtgaggt acttgcgact aatctattag aaaatgaatc ccctcttcct actgccattc
    22561 ttgtcgggag cgatccaatg gcaattggtg tctatcgggc attgaaacaa aaaaatatcc
    22621 aaattggaga agagattgtt gtagcaagct ttgataatat cgagacaatc gcaaacctct
    22681 cccctggctt gaccagtgtt caaatcaatg cgaaatcaat tgggcaagca gctgttcgaa
    22741 tggcattgga gcgaattgac ggtatacggg acgaaccaat cattttgact tatcctgcac
    22801 gattgattgt tagggaaagt tttatcccaa gaacaaaaag gtattgaaag agaggttgtg
    22861 aaaagccgct tcgtttcttc atactcaaaa cgaaacggct ttttcacaac ctcttactta
    22921 tacttattca caaaatccaa tgtctgctgg taacaatttt gcgattcttt tagcgtgtaa
    22981 tcaaattggt attcatgggt aatctctttt tttgtatctg tataaaacaa ggattgcacg
    23041 ggtaccttta attctgtcaa ccgattttct aatgcttgac cttgctcctg gaatgagtat
    23101 gcatttccgt cagttatata cgttggcgga aattccttag aaacatggtc agccacacta
    23161 gcttctttca attgctcact cgttttccaa tcttttgttc caatcaacga ccacgcaacg
    23221 gttttgacga aaaacttcat aaatcgatca tccgaatgct gctgtgctgt tagcttcaaa
    23281 tcaacaggtc cgcaataaga aatggttcct ttcagactat cttttggaag tagctgtttg
    23341 attttcattt gtttagcata cgcatcattc gtttgtatag ctgcatattg caatgcgatt
    23401 tgtgaacctg cactatctcc accaaaaaat aattgttcca tgtctagcaa tttgtcgtct
    23461 ttttcttcag ctaataaatc tttgatcagt tcattcactt gaagaacttg attagggtat
    23521 tctgaatcag gcgctaattc ataattcatc gcaaccactg cgatgttggc gtctgaaact
    23581 aattttgtcg caaattcttt gacacccgat ttatctccgc cgacgaatcc tccaccatgt
    23641 acccaaaata gaacaggaac aggccccttg ctgttttttg gatagtagat gtcataagta
    23701 ttctctttat acttggactc atacgtcaaa tctgtttttt tcgccacgtt tttttctgct
    23761 tgatcgaacg tttttttatc agtgatttga acagtatttg aaaacatatg gttgatcaag
    23821 aatgctccag gtctggggga aagctggaag gcaagaaaga ttgcaaacgc gatcccaaga
    23881 atgattccga agaaccatcc gataaattta agaattttct ttttcataag gctccctcag
    23941 tagttagtat taagcattct gattatacca tagtgttatt tcctatcaag ctctcctaga
    24001 tgatttaatg attttcccca aacttcttct gcaatgcttt tgacaaaaga aattttttcc
    24061 attgttcttc aaacaaaaca ttttcttctt ctgttgaagc aaatccacat tgaggactta
    24121 agcacaaacg atccaaaggc agatatcgtg tcgcttcgta gatccggtca atcacttgct
    24181 ggtgctcttc catttcgcca tttttcgtta cagtcaaaat ctaagacaac taatttatct
    24241 tcagagactt tctctaaaag aacaaaactc ccagcacgtt cactatcgta ttctaaataa
    24301 taggcacgga cattttcttg agtaaaataa tgggtctgct acgtcgtcat cccccctgat
    24361 gccgccaaaa tcgaataata atttccgcgt caaacatgta tatttagtgt tgaatcattt
    24421 gagaatatga aaataaaaat ctttttgatc aatcagttaa tcaaattgaa ataacgaaaa
    24481 atctgagaca gaatgacgtt cattctgacc cagatttgat tttgcacaac tcaacagtcc
    24541 ttattggcaa atcgccaaaa taatgtagac taactaaa
//
LOCUS       DACGLW010000041        24437 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.041, whole genome shotgun sequence.
ACCESSION   DACGLW010000041 DACGLW010000000
VERSION     DACGLW010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.041"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            complement(369..1487)
                     /locus_tag="IXT53_001611"
     CDS             complement(369..1487)
                     /locus_tag="IXT53_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905441.1"
                     /translation="MAKKDKELLTQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            1958..3265
                     /locus_tag="IXT53_001612"
     CDS             1958..3265
                     /locus_tag="IXT53_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAQ4905442.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWGDKVSISDYAENLSIT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETDRLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAD
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            3384..4583
                     /locus_tag="IXT53_001613"
     CDS             3384..4583
                     /locus_tag="IXT53_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HAQ4905443.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYGSTWTFTVI
                     LTYFVVLEIFYSTFYFLIPLLFFSIFAFSYFTEKRRLLNGLLFNVLLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVLLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCVPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYRAQSRATLNPPILLMSGGQGADEKVPE
                     AIAMKQYAMEQGIPERDILVETNSTTTLENMLYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYLAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(4610..5878)
                     /locus_tag="IXT53_001614"
     CDS             complement(4610..5878)
                     /locus_tag="IXT53_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HAQ4905444.1"
                     /translation="MEKQLPSPVRILVYMLKWLVITAILGIFMGSLSAFFLNSLTVVT
                     DIRLSHPWLLCLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNTARLFRLDKSEREILIISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVCRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHVYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAALA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAVYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRMKEKNKV"
     gene            complement(6041..6687)
                     /locus_tag="IXT53_001615"
                     /pseudo
     CDS             complement(6041..6687)
                     /locus_tag="IXT53_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FusB/FusC family EF-G-binding protein"
     gene            complement(7095..7598)
                     /locus_tag="IXT53_001616"
     CDS             complement(7095..7598)
                     /locus_tag="IXT53_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HAQ4905445.1"
                     /translation="MTEKWREDEEYMSYVQDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(7721..8485)
                     /locus_tag="IXT53_001617"
     CDS             complement(7721..8485)
                     /locus_tag="IXT53_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HAQ4905446.1"
                     /translation="MKEIQSVKNPRIKELKKLHKKKYREEKAEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAVNKEISTFLVSDEVMTAMSDLPTPQGILAVMTLTSE
                     DTPDFSGGWLLLDNVQDPGNVGTMIRTADAFGMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLAEVIPVMKGKGFTVYGTELNEEAHALDKVEKTEDFAIIMGNEGQGVSQ
                     EILSSTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            8592..8867
                     /locus_tag="IXT53_001618"
     CDS             8592..8867
                     /locus_tag="IXT53_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HAQ4905447.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            9583..10536
                     /gene="yidC"
                     /locus_tag="IXT53_001619"
     CDS             9583..10536
                     /gene="yidC"
                     /locus_tag="IXT53_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HAQ4905448.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVAIAGGAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVIMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAAKPADNKQATKQIKQPKNSTNNKGRNAGRQQKRK"
     gene            10658..11867
                     /locus_tag="IXT53_001620"
                     /pseudo
     CDS             10658..11867
                     /locus_tag="IXT53_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017466553.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
     gene            complement(12177..12398)
                     /locus_tag="IXT53_001621"
     CDS             complement(12177..12398)
                     /locus_tag="IXT53_001621"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ4905449.1"
                     /translation="MFKNYTINQKILPLDMEQYISETDVAFAVNALVEEKIKVDLYVS
                     SKFTNVKVVGADLFVVNVRKQKVSETDKF"
     gene            complement(12559..13080)
                     /gene="tnpA"
                     /locus_tag="IXT53_001622"
                     /pseudo
     CDS             complement(12559..13080)
                     /gene="tnpA"
                     /locus_tag="IXT53_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631987.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(13171..13537)
                     /locus_tag="IXT53_001623"
                     /pseudo
     CDS             complement(13171..13537)
                     /locus_tag="IXT53_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875519.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
     gene            complement(13551..13836)
                     /locus_tag="IXT53_001624"
                     /pseudo
     CDS             complement(13551..13836)
                     /locus_tag="IXT53_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
     gene            complement(13842..14036)
                     /locus_tag="IXT53_001625"
     CDS             complement(13842..14036)
                     /locus_tag="IXT53_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905450.1"
                     /translation="MAETRPKGYPKLKEYTPSRFMLSECHYDKARADRAVNFIGQLRH
                     TKGKWAGNRFWLLPWQEQII"
     gene            complement(14036..14512)
                     /locus_tag="IXT53_001626"
                     /pseudo
     CDS             complement(14036..14512)
                     /locus_tag="IXT53_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004070864.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
     gene            complement(14980..15333)
                     /locus_tag="IXT53_001627"
     CDS             complement(14980..15333)
                     /locus_tag="IXT53_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905451.1"
                     /translation="MAMSEKAKKYLKEIKGAKTIPELKDVEIAIKRDGILAWAEFTKL
                     NEAVEEKKVALRKKKQETSLQEILFWAYKKESDKLLKMMDEGADKDAIQMQVQRYDSI
                     GQIIAEADLEDEYEV"
     gene            complement(15461..16000)
                     /locus_tag="IXT53_001628"
     CDS             complement(15461..16000)
                     /locus_tag="IXT53_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905452.1"
                     /translation="MQLLLYRIGDSTKIRFLINVRFRVIYSTVLPKGIPQRQKWMIHM
                     EEIMQMILGNAKFQLGQVVSTPAVLETFNPLQTANGIPEALSNLWFRQEISIAEIEDA
                     VLDAKNGKELANSLNRIKLFRKFTLDRELDGMVRLKGTDVWKNISYLKVYTATPPKKP
                     TPPRFQSNGWSLFFSHKSA"
     gene            16030..16221
                     /locus_tag="IXT53_001629"
     CDS             16030..16221
                     /locus_tag="IXT53_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905453.1"
                     /translation="MIIRDDHIYTCDSCHYSFPADEQPERCPDCEKTATRLDTEIETE
                     DYYRVRAEIKAEIKALNAG"
     gene            complement(16263..17129)
                     /locus_tag="IXT53_001630"
     CDS             complement(16263..17129)
                     /locus_tag="IXT53_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ4905454.1"
                     /translation="MEELFSNALKSVKSNRVLYTASFFARSSLLYLQEIGQLTALKAH
                     TSRRTGLSSYLCFLVEVGSGTLEYKGQKYTLQVGDMVFIDCLKPYVHESSEDDLWTLR
                     WCHFNGIGMTEVYKKYLERGGQPVFKTSKTDKYADLLRSLHMVAEGQEYVRDMKINVL
                     LSELLMHLMEDSWNPDKVPNFSTKKMELQNIKQYLDDHYTEKITLDQLSAEFFINKQY
                     LAKIFRETYGTTVVGYLNQKRITQAKHLLRFTNLSMEEIATKIGITDANYFSRTFFKV
                     EGVRPSEFRKMW"
     gene            complement(17153..18805)
                     /locus_tag="IXT53_001631"
     CDS             complement(17153..18805)
                     /locus_tag="IXT53_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HAQ4905455.1"
                     /translation="MNYKVEYERWLNHANADIIEELRSYSEKDIEDSFYKNLSFGTGG
                     LRGTIGAGTNRMNVHTVGKASQGLADYLNKNYEAASVVVGYDSRIKSDVFAKTAADIF
                     AANGIQVHLWPELLPVPTVSFATRYLKADAGVMITASHNPSKYNGYKVYGADGCQITT
                     EAATAILAEIEKLDIFADVKMDGDASKIASIQSEVLTAFIEEVKGQSVLFGEEVDKNV
                     AIVYSPLNGTGLVPVTRTLAEMGYSNVTVVEEQRLPDGNFPTCPYPNPEIKEAMSLGM
                     EYAKKYNADLLLATDPDCDRVGIAVKSAHGDYVLLSGNETGLLLLDYICSQRIKHGKM
                     PADPVMVKTIVTMDLGERIASNYGVRTIDVLTGFKFIGEQIGMLEKAGKVDSYIFGFE
                     ESYGYLSGSYVRDKDGVNAAYLICEMFSYYKTQGISLLDKLNEIYTKYGYCLNTLHSF
                     EFDGAAGFTKMQEIMVEFHKGLDIIGGKKVIETLDYSKGLNGLPKSDVLKFMLEDNCS
                     VVVRPSGTEPKLKTYISVSAENKEAAEAMEAIMAAELAEMFE"
     gene            complement(18810..19712)
                     /locus_tag="IXT53_001632"
     CDS             complement(18810..19712)
                     /locus_tag="IXT53_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HAQ4905456.1"
                     /translation="MMNLQKELRCLLLRKLGMLYAPGWTELCCVWSEYQGMTLTEVLQ
                     EHPEFLGTHPQTKGELPILIKFIDAKKDLSVQVHPTDEYAIEKENGQLGKTEMWYVLD
                     ASLIYGLQKITEEAVIREAIAQGTVERYLQKVPVKKNDVFFIEAGTIHAIGAGALIAE
                     IQENSNLTYRLYDYDRVDKNGKKRELHVDKALEVANLKGLSEPKQPLRVLKYRAGVAA
                     ELLCRCKHFEVSRMIVNTERRQTVHYRADSISFRVLLCVNGCGTVRFDNESLDIYKGD
                     CLFVPADSTEISIHGSIQFLDVRG"
     gene            complement(19796..21109)
                     /locus_tag="IXT53_001633"
     CDS             complement(19796..21109)
                     /locus_tag="IXT53_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HAQ4905457.1"
                     /translation="MNIVLLSGGSGKRLWPLSNDIRSKQFIKIFKTEDGYESMVQRVY
                     RQIKKVDNDATVTIATSKTQVSAIHNQLGEDVGVSVEPCRRDTFPAIALATAYLVDVM
                     GIDPEESVVVCPVDPYVEDDYFEALKSLSDQADKGEANLVLMGIEPTYPSEKYGYIIP
                     ESAEQTAFVNTFKEKPSEAVAKEYIAQGALWNGGVFVYKLKYVMERAHQLIDFVDYQD
                     LFNKYDTLTKISFDYAVVEHEDKIQVQRFAGMWKDLGTWNTLTEGMEESIIGKGVMNE
                     NCDGVHILNELDVPVLAMGLHDVVICASREGILVSDKEQSSYIKPFVDKFEQQIMFAE
                     KSWGSYKVMDVEAESMTIKVTLNASHSMNYHSHKNRDEVWVVLAGTGKTIVDGYEQVV
                     KAGDVITMSAGCRHTIMADTELKLIEVQLGKDINVKDKQKFELEY"
     gene            complement(21177..22121)
                     /locus_tag="IXT53_001634"
     CDS             complement(21177..22121)
                     /locus_tag="IXT53_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019150212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HAQ4905458.1"
                     /translation="MMEKDAKIYVAGHRGMAGSAIVRELNRQDYNNIITRTHKELDLC
                     RQDAVEAFFAQEKPDYVFLAAAKVGGIIANQNALADFMYENMILEMNVINSAWENGCK
                     KLQFLGSSCIYPRMAPQPMPESCLLTSELEKTNEAYALAKISGLKYCEFLNKQYGTDY
                     ISVMPTNLYGPNDNYHPTHSHVLPALIRRFHEAKEAGLPTVTCWGDGSPLREFLYVDD
                     LANLCVFLMNNYSGDETVNAGTGKELSIKELTEMVAKVIGYEGEILWDTSKPNGTPRK
                     LLDVSKATKLGWTYKTELEDGIRLSYEDFLNNPMRAER"
     gene            complement(22158..23171)
                     /locus_tag="IXT53_001635"
     CDS             complement(22158..23171)
                     /locus_tag="IXT53_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905459.1"
                     /translation="MATLCRCEAKITLVCGANHLDFAKRNLEQELPKELIDRVRYRPI
                     NTDIGLINREGTLLVDTGKLTEICEYFLAKVPSLAKTEAEWLKREAVSAVLCDMPIWA
                     MDACDMAGVPFLYVGNFTWTELYREFLPEHIWKEYAKRYGKIQHTMLYALNNEEMLEF
                     LQNSEKTPTSVVARPIHNDEVQRIKSQHEKPPVFVALGMSARFKQPIDVSHAPYDFIT
                     TLGVPLVGENVTVISNSTPNTQDYIAAADYVITKAGWGTVAECILAQRPMALFARDTV
                     LEDRTTICKLQKLGLAISVTPENLHRVERVIEDLKQLDTSNFDTFYDAASEIADKLIS
                     LMQ"
     gene            complement(23251..24297)
                     /gene="gmd"
                     /locus_tag="IXT53_001636"
     CDS             complement(23251..24297)
                     /gene="gmd"
                     /locus_tag="IXT53_001636"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HAQ4905460.1"
                     /translation="MKKALITGITGQDGSYLADFLLEKGYEVHGITRRASISNTARID
                     HLMGKITLHDGDLSDSSSLIRIISIVQPDEIYNLAAQSHVQVSFDVPEYSGDVDALGV
                     MRILEACRILGLTKKTKIYQASTSELYGKVEEVPQRETTPFHPYSPYAVAKQYGFWIT
                     KEYREAYGMFAVNGILFNHESERRGENFVTRKITLAAGRIAEGLQDHLELGNMDSLRD
                     WGYAKDYVECMWMILQHETPEDFVIATGEQHTVRDFTEKAFAANGMTIRWEGTGIDEK
                     GYDVETGKMLVCVNPQWFRPTDVDNLWGDPTKAKTVLGWNPQKTSYEELVNIMAKHDR
                     ELAKREAAMKNAVK"
BASE COUNT         6949 a         5537 c         4652 g         7299 t
ORIGIN      
        1 ttttttaaac ggtgttccag aagtagcttc ttcggaaata agccgaaatt tcacaaaaat
       61 ttgagaaata atttttgtca atctcttctt atttcttgaa gctgaccact tctgtcacaa
      121 cctcttcctt aaacggtgtt caaaagcttg catctgcggt aataagccca ccaaacaaaa
      181 aatatgaaga gctatttttg cttgctgttc ttattacttg atgcagaaca gctttttcac
      241 aacctctcat acacggtgtt taatcagctg accttcggta ttagctcaaa aaacggaaaa
      301 atatgtgaaa ctatttttcc gtttgtttct ctaatactca ggtcaaaaca ctgatttcac
      361 aacctctttt aataacgaag aatcgctgtc agactctttt catctggcgc atctttttga
      421 tccaaaataa acactttccc gccattaatg atcacatcat ctgctaatgc attcaacact
      481 tgacgccgat catattctgt ttcaggatct tcaccgaaac catcagctaa gtttgacgta
      541 gcgataaata aatgtgacac ttttccttct ttcgcagctg gaacgatatc aactaactga
      601 tcgatgaatt tcttatctaa gagtttattg tattcttttg attcttgagc tgcgagttca
      661 cttgacagct tcttcgcccc ttcttcgata tctctaaagg atagctgcgc aggagaagca
      721 gaaatcgcaa cgtctgcaca ataataggga tttttagcaa tttttttgaa catcgtctga
      781 ttttctggta atgcaaataa ataaatcggc cattgttcag gattttcgaa agaatccttc
      841 aagtacgtat caacagcttg gtaatagttg acccaatcga tctctacttc ttcatctttt
      901 ggatttacgc catggaaagt tactccatct cgggctccgc ctcctccatt agaagagaag
      961 ttcaaaccgc cacctgtcag ttcagatccc aatgcagtaa cgacatcaac cggtgcccct
     1021 tctggtagag atacttcttt tacttgtttg ttgctgacct catacaattt catggagtcg
     1081 cggttcaatc ccaataaata ataagaatag ttgaactgcc catttttgat aatagccagt
     1141 aaataaggaa gatcacttac atagtattga tcgtctaccg ggatactcaa acgatggata
     1201 taggtctggt cagcagttat aatgaccgct acactcgtag ttgcactacg ccaaaacgaa
     1261 ggatctccta acaaattttc aattttttct tggaacgggg accagtcatg ttcggaatat
     1321 tttttctcaa aacgtttctt tgcttctttc gcaaaatttt tgaatttgat ctgatctttt
     1381 tccacatctt gatgggcgac atgcgtattc agcatgatcg tgacgaatgg tcctttagcg
     1441 ccttctgaag tcaattgtgt caataattct ttatcctttt ttgccataaa cgtacccctc
     1501 ctaaaaaaat cattcctttg tttctatcta aagtctacca caacactatt tttccataca
     1561 ataaaacgta tctaaaaaaa taaaacggtt tctacggttt taaatttagc tattttttta
     1621 agttatcttc ataatttcct caattattta gccccagtct tcaactattc ttcatgaaat
     1681 gctgataaac taagaaaaag aataaaagag gtggatctta tgcatttgat tatgattttc
     1741 agtttattaa gtattatcag tttatttgct ttcagcgctt cccgcttatt agatgcggaa
     1801 acacagccta ttcctgtaaa agtacgtaaa aacgaccgct aaacttccat aacgcgcgtt
     1861 cttatcctaa agttttttca gaaacattga aactctctcc cctctctgat aaaattttaa
     1921 tttgtagcga aacaaattaa ttacaaggag atcggatatg tccataaaaa aaacacgtct
     1981 agtcctatta ttggctagtc tcattctttg ccttgcttct tttctaccag ctgttgctgt
     2041 gcaagctgaa gatacgttca aagtaaatgc aaaagcagct tttgctgtag atgcgcaaag
     2101 cggtaagatt ctttatgatc aagatggaga aaaaaccatg gggatcgctt caatcacaaa
     2161 aatcatcggt ctttacatcg tccttgatca agtaaaagaa ggaaaactgt catggggtga
     2221 taaagtttcg atttcagatt atgcagaaaa tttgagtatc accccagatt tatcgaatgt
     2281 tcctttgcat aaagaaaaca cctacactgt caaagaatta ttcgactctg caatcatcca
     2341 gtcagccaat gcttcaatgg tggccttggc agaaaaaatc tctggcagcg aagctaaatt
     2401 cactgaacga atgaaagaac aattgaaaga ttggggaatc aaagatgcaa caatcgttaa
     2461 tgcatcagga ttgaacaact cttatcttgg cgaaaaccga ccagaaggca ctggagaaaa
     2521 cgacgaaaat caaatgtctg ctcaagatgt ggccatcgtt gcccgccatt tgatcttgga
     2581 tttcccagaa atactggatg tatcaagtac tacgacccaa atgtttggcg aaaatacgca
     2641 atcaccagtg gaaatggtca actggaactg gatgctccca ggattcgtta attacaagga
     2701 aggcgtggac ggattaaaaa ctggtacgac tgagcttgca ggtgcttgtt tcgttggtac
     2761 gatcactaaa aacgatcaac ggatcatcac tgtcgtattg aatgcggacg gccatgcgga
     2821 aaacccgagt gcccgcttca atgaaacaga ccgtttgatg gactactgct atgacaattg
     2881 gtcagaaaaa gagctcggaa aagctaacgc aagtattcct agtctaaaaa ccattgatgt
     2941 aaaagacgga aaagaatcaa cagttcctgt tgtgcttaaa agccctgtaa aagtatgggt
     3001 acgaaacgat atggatactg gcaaattaac gatcacgcca acacttgaca agaagcaagt
     3061 cactgacaat gagatccaag cacctatgga taaaggtaca aaaatcggta ccgctacgat
     3121 caccttggca gacgataaat taggctattt agaagacaac aatgcaccat caacagagat
     3181 cattacggat aaaacagtgg aaaaagcgaa tatattcgtg attggctggc gacgcgtcgc
     3241 agaatttttc ggcaacttgt tttaattgaa caataagaaa aacatctttc catcggaaag
     3301 atgttttttt gctttttcta gtttaaataa cagctttttt catctatttt cgttatactg
     3361 tataagaaaa aggagttgaa tttatgaatc taaccagaga tctgatcttt ttagttctct
     3421 atcttattgg ggtattctct atcgtttatt ggcgaaaaca gaagaaggat ccttccttgt
     3481 tcttttacgg cagtacgtgg acatttacgg taatcttaac ttattttgtc gtgttggaaa
     3541 ttttttattc gaccttttat tttttgattc cattgttgtt ctttagtatc tttgcttttt
     3601 cttatttcac agaaaaacga aggctactga atggtctttt attcaatgtt ttactcatca
     3661 gtttcggcat ctacttgttt gtcttgctgt atgaaacgca aaatattttt cttggcggac
     3721 tgatcgcctt gatcacgatt ccattgctac ttgtcctttt atttggtatt tatggattga
     3781 ttgtttttct tttttggaac ggggtcacag ttttacgtag agaatcgcat tcattagcta
     3841 atcttttaac gttgatacta gccatttttc ttacactgtt tcttgttttt gactttttct
     3901 tgttaaaata tttgccgcaa tggataaatg ctttgttttt ctgcgttccc ctgatcttga
     3961 tctatctatt tatcgtattc tacaactttt tgactgtctc attcttgtat cagttcaacc
     4021 ggcctcgata caatcaagac ttcatcgttg tgttaggcgc tggattgatc aatggagaaa
     4081 cagtttctcc tcttctagcc aagcgaatca ataaggcaat tgctttttat cgggctcaaa
     4141 gccgtgctac attaaatccg cccatcttat tgatgtctgg cgggcaagga gcagacgaaa
     4201 aagtaccaga ggccattgcg atgaaacaat atgccatgga acaaggaatc cctgaaagag
     4261 acattcttgt cgaaaccaat tcgacaacca ctttggaaaa catgctctat tcaaaggaaa
     4321 tcatggatca gcaaatgaaa gggccatacc gcgcgatctt tagttccaac aattaccaca
     4381 tttttagagc cggtctttat gcgcggcaag ccaaattgaa agcaaatggt attggtgcaa
     4441 aaacagcttt ctactacttg cctaatgcgt ttttaagaga atacctcgcc atcttgatga
     4501 tgaacaaaaa aagacatatg atcatttgcg gattgctttt cgcaggctcc gcattcttat
     4561 cgctgattac tttacttttc taaaaaaaca aggtgtgaca aaacatatgt cacaccttgt
     4621 ttttttcttt cattctctta actattggca aaaagagtat cccttttttg accgctactt
     4681 gctgagcaga ataaataccg ctgttgcctg aacacataaa gctgatcaag cagatcataa
     4741 agaagtatac tgccgcttgt gaaccgaaca attcgatccc catcacaaag caggcaatcg
     4801 gtgtatttgt cgcaccagaa aatactccga taaagcccaa tcctgcaaga aaaggaacag
     4861 aaacatggag caaaggcgct agtgctgcac caagcgttgc accaatttca aacaatggtg
     4921 tcacttctcc cccttgatat cccgcgccta aagacaaaac tgtgaagaat aacttcccaa
     4981 taaaatcata gacatgtgcc tgtccattaa atgattcttg aagtaacgga agactcaatc
     5041 cgagataacg ttgtgtttgc aaaatcaatg ctgctgccac aactacagct ccacctatcg
     5101 tatttcgaca aacaggatga ggaatccatt gtgcatatcg cgcttttaaa aagacgatcg
     5161 aacggctgaa tacccaacca atcaaaccaa aagcaatccc agcaacaaaa actttgaaga
     5221 acagcaacat actccattca ggaatcacac ccatttggta atgtagatga gaaacaccat
     5281 agctttctgt catgaaattt gcaaacagtg cagcaaagaa gctagggaag atcgcttctg
     5341 ttcggacttt cccgattgcc aacacttcta atccaaataa tgttccagct aaaggtgtgc
     5401 caaatacaga actaaaaccc gcactgattc cactgataat caagatttcc cgttctgatt
     5461 tatccaatcg gaacaaacgt gccgtattat ctgctaacgc tccccccatt tggactgctg
     5521 ttccttcacg gccaacagat cccccaaata aatgggtagt tatggttcca aacagtgtaa
     5581 gtggaataag ccgcaacggg attttttctt ctccaccatt tccttgatcg atcactaagt
     5641 tgtttccacg actagaaagt ccgccgtgat acgcatagag ataagcaaaa agcgcaccac
     5701 taactggtag tagacaaagg agccaaggat gtgatagtcg gatatctgtc acaactgtca
     5761 ggctatttaa gaaaaaagca gacaagctcc ccatgaatat accgagaatc gctgtgatca
     5821 ccagccactt cagcatatac actaaaatgc gtactggact cggtaattgt ttttccataa
     5881 aaaattcctc ctcaataaaa aaacgcctac taaagaaagg ggctgtcccc agactttagt
     5941 aggcgtcatt agctatcttt cgttagcatt taaaggcgaa caccatcacc tattcacttc
     6001 cattaaaaga tagcatattt ttctgtaaaa aacaataaaa ttatcgttct ttctttacac
     6061 gttcagcaaa ttcatagaaa ggagccaatt tcgttaactg ttgattacat tggtcactat
     6121 catgacaaat ataattcccc tttttcgtat acgtgccatc agaacctgct ttcgttgtag
     6181 aaagaaaaag cgaaacattc gatgttttct gacagattgc acagattccc ttatttatcg
     6241 ttggtgataa agtaccgcta atccctttta atttttcatc ttcgtaatag accatgaatt
     6301 ttttctgcgt acctggatca ttccagccga tgtacgtatg ttcttgcaag tcgatttcat
     6361 tccaagctgg ttgtttgagt tttttcactt ttcggaataa tttctcgatt tgtggtttgc
     6421 taggctgcat aaacggctga acgatttgct tcagctcctc caatgctctt tctgcttgcg
     6481 cttttgtcag ttttacgtct aaaactgtcg ttaaaaatgt atcgattctg gcagttcgtg
     6541 atctaatatg tctcgaatct tttcagctgc tagtgcctga actgtttgta ccgtttttgg
     6601 atcattgaca gatgagtacg catttaataa ctgttctact tctcttatta taaaaaaata
     6661 ttggtatggt tttattgttt gttccatgat tttaaagtcc tttcttctaa ccaaagtgca
     6721 tactttgtag aaaaaagtga tcttctgcaa agtatgtgtt acctgaagat cctggactct
     6781 tttaaataag atacgttttg catcttatca tcaccccctt taatgcttat tattctatgt
     6841 acatgattca ttcgataaat aaaagtaaac cgtatcttga acagatagtc aactgcaaac
     6901 agaaaaaagg actgtgacaa aagtcgtgtc aaagtccctt gtttcgaatg aatggtgttc
     6961 aaaagcttgc atctgcggta ataagccgaa acttcacaaa aatttgagaa gtaattttcg
     7021 taaattcctt cttatttctt gaagctgacc acttctgtca caacctcaat atggtctatt
     7081 attggtttta cctgttaaat cgaggattct ttcccaaagt aacgttgcca tctcttacgc
     7141 atacttgcag acatgggctg agccgcttct ttgactgcgc agtatttgtc cacgaatgtc
     7201 acaatatatc cttctttata cttaggcggt gcgatcgttg caccccacat gtgcttcaaa
     7261 atgatatctc gctcaagatc cgacaaatcg gtgatttttt ctgcattctt tacagcaatt
     7321 cttggatgga tatacgcatg tgtcccctcg tcaaatttcg tcgtacgcca atcatagtag
     7381 aacagatcat gcaaaagtcc agcacgcgct gttgctttag cattgccacc ccattttttt
     7441 gcgagcagat aactatagta agaaacactg atggaatgct ccaaacgagt ggaatgaaca
     7501 tgctgtgtgt aattcgctaa ttttttcact gcttctgttt ctaaaagatc ttgtacataa
     7561 gacatatatt cttcgtcttc acgccatttt tctgtcataa ttatcgattt gccacctttt
     7621 tgaatttcat tttcttctga aattgatcta agtataccac cagtgttcgg attattctag
     7681 tcgctctaga taaaatagct aaagcttaat agttccttaa tcataaagaa aaatgataaa
     7741 gaacgatccc tgctgcgact ccgacattca atgattccgc atttcctttc attgggatat
     7801 atacgttctg atcagtcgaa gacaagattt cctgacttac tccctgacct tcgtttccca
     7861 tgattatagc gaaatcctct gttttttcta ctttgtctaa tgcatgtgct tcttcattta
     7921 attctgtacc gtacaccgta aaccctttgc ctttcattac agggatcact tctgccaaat
     7981 ctttttgtaa cacaggcaaa tgataattgc ttccctgcat gcttcgcagc acctttgtac
     8041 tataaatatc tgccgttcct ttacctaata aaacaccagc cataccaaac gcatctgccg
     8101 tacggatcat cgttcccaca tttccaggat cctgtacatt atctagtaaa agccagcctc
     8161 ctgaaaaatc tggcgtatct tcagatgtta acgtcatcac tgctaagatc ccttgcggtg
     8221 taggtagatc tgacatagcg gtcatgacct catctgaaac tagaaaagta gaaatctctt
     8281 tgtttacagc cagccagtct ccccattctt tcatacctct ctgtgtcaca aaaagttctt
     8341 ttatttctgc atcagacgac gcagcttctt cgatcagatg gaacccttcc aaaagatatt
     8401 ctgccttttc ttctctgtat ttctttttat gtagtttttt taattctttg attcttggat
     8461 tttttactga ttggatctct ttcacattcc tcacctcaac tgattatatc atggaatagg
     8521 tgcttgagct tgtttcatcc taaaaaaatc ggtatcattg atttataaaa caggaaagaa
     8581 aggtgacgaa catgcgcaaa gttaaaatga atgtccaagg acgtgtccag ggcgtgggat
     8641 ttcgctatat gaccaagatg gttgctgatc agttaggcgt gaccggaacg gtaaaaaacg
     8701 aagacgacgg ctcagtcaca atcgaagcaa tcggaaatga cgacatcatc caaaaattta
     8761 tcgaagaagt caagaagtct ccgagcccaa gtggacgagt gcagtatgtg gatgtccaag
     8821 aagatccgat gattgaggaa cgaaagaaat ttgatgtggt gggataaggt tgtttttgaa
     8881 gcgctttgcc tcgagtatta aggagaaaca ttttccaaat agcttctcat attttgaaat
     8941 gtaatgactt aataccgaga ggcagctgat aaaacaccgt caataaggtt gtgaaaaagc
     9001 tggtctgcat caagcattaa ggactggttg tgaaaaatag ctctccatat ttttcaacag
     9061 gctggactta atgccgtaga tgcaagcttt tgaacatcgt cgataaggtt gcgaaaaagc
     9121 tgatctgcat caagcattaa ggactggttg tgaaaaagtc gcttagtttt gagtattaag
     9181 gaaaattttg tgaaaatggc tttccacatt tttgcataaa tttgacttaa taccaagaaa
     9241 ctgacttttg aacatcgtcg ataaggttgt ttttgaggca ctttgcctca agtattaaag
     9301 acaggtagta aaaaagctga tctataactt gcaataaatt tgcttaaccc atattttcca
     9361 gacagaacaa aaaagttttt agttttttaa ataagaaaga aacacttgga atcactcttt
     9421 tctttctaag tatttctttc ttgatttttt gtcttttttc aaaatttttc gctacgaaaa
     9481 agaacgaata tttatatgaa atttctatga agttgattga attccatgcg cttttttagt
     9541 atagttaagt ttgtgtaaaa attatagaag aggaagtatt ctatgaataa atggaaaaac
     9601 tggttattgg gatccggact tgtcagtatc ctgctctttt tatctggctg tgtgcggatg
     9661 gattcaaacg gaaaccctga cacttcagga atcgtttatc gagtactcgt ccatcctatg
     9721 ggccaagcga ttacatatct agttgagaat ttcaattggt cttacggctg ggcagttatt
     9781 gcaatgactg tgatcgttcg tatcatcatc ttgccactag gtatcagtca atcgaaaaaa
     9841 acaatgatcc aatctgaaaa aatgcaagcg ttaaaaccac aagttgaagc agcacaacaa
     9901 aagctaaaag ctgcaacaac acgtgaagaa caaatggcag cacaagcaga aatgcagcaa
     9961 gtatatcgcg aaaatggatt gagtatgacc ggcggaatcg gctgtctgcc tctattgatc
    10021 caaatgccga tcttctctgc cttgtatttt acggctcgct acacagaagg tatccgtgaa
    10081 tcatcttttt acggcatcga tctaggctca ccaagtatgg tgcttgtggc catcgcaggg
    10141 ggcgcttatc tgcttcaagg atatatttca acaatcggta ttcctgaaga acagaaaaag
    10201 acgatgcgta cgatgctgat cgtctctcct gctatgatcg tcttcatgtc gatcagtgcg
    10261 ccagccggtg ttacacttta ctgggtagtc ggtggtgtgt tcagctgtct gcaaacattc
    10321 atcacaaacg ttataatgaa accgcgtttg aaagcacaag tggcagaaga gttgaaacaa
    10381 aacccgccaa aacaagtcgt tactccaaga aaagacgtga cagaatcagc agcaaagcca
    10441 gctgacaaca aacaagcaac aaaacaaatc aaacaaccaa aaaacagtac aaacaacaaa
    10501 ggccgcaacg caggaaggca gcagaagcgg aaataaaaat atatgacaaa aaactgtaga
    10561 caaataaaaa aatttctttg ttctacagtt tttttgttac aattaaagaa aacgttcgca
    10621 tttcgaggag gaatgtataa tgatgtccaa ataatcaatg tatagtcgaa tggaagaaga
    10681 caaaatttcg ctagatgcac ttgttcctaa aaatcattta gtccgtaaaa ttgacaaatt
    10741 attgtcattt gattttattt atctcattgt tgaatcaact tactcgacca ttggtcgccc
    10801 tagcgttgat cctgtcgtct tagtaaaatt agtattcatt caatatttat ttggaatttg
    10861 ttcgatgcgt cagaccatta aataagttga aacaaaccta gcttatcgtt ggtttcttgg
    10921 gcttagttta attgataaag ttcctcattt ttcaactttt ggtaaaaact atgcacggcg
    10981 tttcagagaa accgatttgt ttgaacagat atttatcatt attctcgaat aagcagttga
    11041 tgctagattt attcatgaag aaattttgta tatggattca actcatatta aagcgaatgc
    11101 gaacaaacgt atttttacaa gagatatggc acataaagaa gccattttat accaaaaaca
    11161 attagaagaa gaaatcaatg aggcaagaat tgccgaaggc aaacgtcctt ttggttcaat
    11221 cattcgtcag gaggtggtcg aaagaaaatt tagcatcgca gatccagaaa gtggttatta
    11281 tgtaaaaaca gaacgagaaa aacagtttgc ttattctgct cataccgttg gtgatgaaaa
    11341 tggttttgta ttagatgtca tgattacgcc tggaaatcta catgatagcc gaatgctcgt
    11401 gccacaaatc gaacgcgtta aatcacgtta tggtgtagct gctaatgctg cgtacaaaac
    11461 accttggaac gtcaagtatt taatcgaacg aaaactacga cccattctcc cttatatcag
    11521 tttcgcacaa ctactcgaga tggctataaa aaatatgtat ctaaatcttt tatttgtgaa
    11581 agttgtccat tattgaaaaa ttgtacagaa agccaaaaac accaaaaaga aatacatcga
    11641 catatttggg aagactactt agatgaagca gaacatttac gccatacggt gtacaatcgt
    11701 gaaaaatata aaaaaagaaa agaaacgatt gaacgcattt ttgcagacgc aaaggaaaaa
    11761 catggaatgc gctacaccaa gtatcgaggc ttggaaaaaa tcaggtgcat acaatgcttg
    11821 tttttgtcgc aatgaatctg aaaaaattag ctacttggca gtgggagcat atgtcgttat
    11881 atcttttatt atacaaaaat agacaaaagt atcagtctac actcttagta aagaggaaac
    11941 tgatgctttt gtctacagtc ttagagagtg ggacaatagt tttttcttct ttacgtcttt
    12001 tcctctccat gtattttcct aattttctta atttaacgcc atgagtgcca agccaatgtc
    12061 aatcttggct ccttgtttcc ttcataagtg gaaatccgtt tccgatagat cgaaccttct
    12121 ttttcctcta aaagtttttt tacattctgc tttgaaataa cgccatgtat tattcactaa
    12181 aatttgtcgg tttcgctcac tttttgcttt cgtacattga ctacgaacag gtcagcaccg
    12241 accactttca cattcgtaaa ttttgaagac acgtataaat ccacttttat cttttcttcg
    12301 actaaagcgt tgaccgcaaa agcgacatct gtttcagaaa tatattgttc catatctaat
    12361 ggtaaaattt tttggttgat ggtataattt ttaaacataa agcactcgct tcctttctga
    12421 ttgggtttgg tcactttaat cctacaggga tgagtgcttt ttgtgtacct taaaatcagt
    12481 aaatatccat ccgtgaaaac gggtggcttt ttaacagccc tagaagggct caccactcac
    12541 agccctatct ctctttaact atttaaatgg atctacatac tcttgtttac ttatactgtc
    12601 tctaattcga tcatctgctt cttgttctca aatatatatt tcaattgttt tttgatttaa
    12661 cccaaccgta ctcacgtagt atcctttggt ccaaaagctt ttattcccat acttatattt
    12721 caaatttgca tgtttttcat ggattaaggt tgcacttcgt ccttttaagt agcccataaa
    12781 actagaaata ctaatctttg gcggtattct tactaacata tgaatgtgat caggcattgc
    12841 atgagcttca atgatttcaa catctttcct ctcacacaat tttcttaata tctggccaat
    12901 acttactctt aactttccat aaatcacttt tcgtcgatac ttcggggtaa agactatgtg
    12961 atacttacaa tcccatctcg tatgtgctaa acttttatcg ctaaagctat ttcgtccacg
    13021 cgtagaaacg cgcgtttttt tgtttcgtac gctacgccaa cgaaactgac taaagtcaat
    13081 aaaaaaagtg gggcaataat cagatttttt tctgactatt gtcccactct ctttttatta
    13141 ctcttcatct tctactattc cactaattaa ttagttgaca gatacaatgc tttcacatcc
    13201 tgcacgaaca aactcgggct ctgaaccaat cacttgcgag ttgataaatt caatgaccat
    13261 aggaagatta atccgacata aagtttaatc tgttgtcctt ccattatcag tttagaaatg
    13321 atattagtag gtgtaccgcc taaaagatcg catagtacca caaaatcatt taacgaatct
    13381 atgacagtaa gagcttttgt ctgtatgttt cagtgccatc ttctggtaat aaaggtaacg
    13441 tgtagatatt ctcttgaggt cccataatca tttttgtact ttttttcaat tcctcacaaa
    13501 attttccgtg actaatcaat atcaattttc tactcatttt tggctctccc ttatttcgaa
    13561 atcacacaca atgcagataa agccacacac aagtaatacg ataaaaatcg ctcgagttgg
    13621 gtcatatttt tctttcctaa caaccaataa acacctcgac gatcccagcg agtaagattt
    13681 tagataatat catatcgata tagttttgca tattcaatgt cacatcgcca atcgttggta
    13741 ccataaaaat ttaatatcaa tcattgttgc aactaatgct cctaccatga acactcttaa
    13801 taaagtcgct acgtccgtta atacagttaa gcgagattac actagatgat ctgttcctgc
    13861 caaggcaaga gccagaagcg gttgcctgcc catttgcctt tggtgtgtcg gagctgtccg
    13921 ataaagttca ccgcccgatc cgctctggct ttgtcgtagt gacattccga aagcatgaat
    13981 cggcttggtg tgtattcctt taatttggga tagccctttg gtctggtttc tgccatcagc
    14041 taccacctcc caacagagcc tccatcgcat cttctgctcc gacaccaccg ccatcaccgg
    14101 caatgatacg gcttctggac gaaggcgtca gaccgaactg ctcggaaaat cggttcataa
    14161 ttttcagata ggtctgggca ataaacacct gtggtacctg ctgccaatag ccggataggg
    14221 ttttcacgat gataccgtgc tgggtaatga attcctcggc ttccttccag cgggcgtagg
    14281 cttggcacta accggcaaag gcagccatat ccacatctgt caagattccg atggcctcca
    14341 tctgctttgc cagacgcttc cattccttct ttgcctctgg ttccagccac ttcggacagg
    14401 tcggtgcctt cttttccggc gttgactctt ttgcattgag tgggcgattg cccggatttc
    14461 cctccagctt cttcatggct gtcggggttg attttcttcc tctggttgcc acagtcgtca
    14521 cctcccttca atgtatttct gccagtagac agagaacaga gccaagtcga aacctgactc
    14581 tcttctctat ctgctaactt gtatttcagt ccactcttct gatgaatcgc acccgcagga
    14641 ttttcaagat gccgcaggca aattccaaga agtttctgac cgcatcataa ccatcgtagc
    14701 cgtgtagcca catccttcca cctcgccgcc gtcctcgtcg ctgatataaa tgattctgta
    14761 ttcgctcatc tgttgttttc tccagtcttt tgtttttttg tagtacatat atcactctaa
    14821 aagtgattat tatcaagtcg tttgaaagcc ataatgtaca caatcattcg actggttttt
    14881 ggttcataat tgaccgccaa agtgagcaaa agaaagggac ctctgtctgt ttcagaagtc
    14941 cctttcggta tttggttctt agtggttcag gctgtattct caaacctcat actcatcttc
    15001 gaggtctgcc tctgcaatga tctggccgat gctgtcgtaa cgctggacct gcatctggat
    15061 ggcatccttg tctgctccct cgtccatcat cttcagcagc ttgtctgatt cctttttgta
    15121 ggcccagaaa aggatctcct gaaggctggt ctcctgcttt ttcttgcgaa gggcgacctt
    15181 cttctcttcc accgcctcgt tcaacttggt gaactctgcc caagcaagga taccgtctcg
    15241 cttgattgcg atctccacat ccttcagctc cgggatggtc tttgcaccct tgatctcctt
    15301 caaatatttc tttgcctttt cgctcattgc cattagcgtt tcctccttgt ttttgggatt
    15361 ttcccctttg gttgtgtgta tattgccata gtctctcgac tatatcaagt caatccacag
    15421 gcatatacta cacaatcatt tggagctgta tttgtataca ttatgccgat ttatgagaga
    15481 agaaaaggct ccagccgttc gactggaacc ttgggggggt cggtttctta ggcggcgtgg
    15541 cggtgtagac cttgaggtag ctgatgttct tccagacgtc cgtgcccttc agtctgacca
    15601 tgccgtccag ctctcggtcg agggtgaact tgcggaaaag cttgatgcgg ttgaggctgt
    15661 ttgctagctc cttgccgttc ttggcatcca gaactgcgtc ctcgatctcc gcaatactaa
    15721 tttcctgtct aaaccaaagg ttgctcaagg cttcgggaat gccgtttgcg gtctgcaggg
    15781 ggttgaaagt ttccaataca gcaggggtcg aaaccacctg tccgagctgg aattttgcgt
    15841 tccctaaaat catctgcata atttcttcca tgtgaatcat ccatttctgc cgttgtggta
    15901 ttccctttgg cagtacagta ctatatatca ctctgaatcg tacatttatc aagaagcgga
    15961 tttttgtgga atcaccaatt cgatataata gtaattgcat gaaaaatcta agcactggat
    16021 tggtaggtga tgattatacg tgacgatcac atttacacct gtgattcctg ccactacagc
    16081 ttccctgccg acgaacagcc agagcgttgt cctgactgcg aaaagacagc aactcggctg
    16141 gataccgaaa tcgaaactga agattactat cgtgttcgtg ctgaaatcaa agctgagatt
    16201 aaagctttga atgcgggtta acgacaggta ctgaccggcg gctcaacacc gccggctttt
    16261 tattaccaca tcttgcggaa ttcgctggga cgaacacctt ccaccttgaa aaaagttctg
    16321 gaaaaatagt ttgcatctgt tatgccaatt tttgttgcaa tctcttccat acttagattt
    16381 gtaaagcgta gcaagtgctt ggcctgtgtg attcgtttct gattcagata accgacaaca
    16441 gttgtaccgt aagtttcacg aaaaatctta gctaagtact gtttattgat aaaaaactct
    16501 gcactcaact gatccagcgt aatcttctcc gtgtaatggt catccagata ttgttttatg
    16561 ttctgcaatt ccattttttt tgtagagaaa ttcggcactt tgtcaggatt ccaagaatct
    16621 tccatcaaat gcatcagcag ttcactgagt agaacattga ttttcatatc ccggacatac
    16681 tcctgtccct ctgccaccat atgaaggctc ctcagcaagt cagcatactt atctgttttt
    16741 gatgttttaa agaccggttg accacctcgt tcaaggtatt ttttgtagac ttccgtcata
    16801 ccaatgccat taaaatggca ccaccgcaaa gtccacaaat catcttctga tgactcatgg
    16861 acatacggct tcaaacagtc aataaacacc atatctccta cttgaagcgt atatttctgc
    16921 cccttatact ccaatgtgcc tgaaccaact tctaccagga aacaaaggta agatgacaat
    16981 cctgtacgcc tagatgtatg tgccttcaga gcagtcagct gtccaatttc ctgcaaatac
    17041 aaaagagagg atcttgcaaa gaacgatgcc gtgtacagca cacgatttga cttcaccgat
    17101 ttcaaagcgt tagaaaacag ttcctccatt acaagatttc tcctttcttt tcttactcga
    17161 acatttccgc cagttcagca gccataatag cttccatagc ttcggcggct tccttgttct
    17221 ctgcgctgac gctgatatag gtcttcagct tcggctcagt accagaagga cgaaccacaa
    17281 cagagcagtt atcttccagc ataaacttca aaacatcaga cttcggcaga ccattcaggc
    17341 ccttactgta gtctaaagtt tcaataacct tcttaccacc aattatatcg agacccttgt
    17401 ggaattcaac cataatttcc tgcatcttgg taaaacctgc cgcaccgtca aactcgaagg
    17461 aatgcagggt gttcaaacaa tagccatact ttgtataaat ctcgttcagc ttatccagca
    17521 ggctgatacc ctgagtcttg tagtaagaga acatttcaca gattagataa gcagcgttaa
    17581 caccgtcctt gtcacggaca tagctaccgg acagatagcc gtaagattcc tcaaagccga
    17641 agatatagct gtcaacctta ccagcctttt ccaacatacc aatctgctca ccgataaact
    17701 taaagccggt cagcacatcg atggtacgaa caccatagtt ggatgcgata cgttcaccca
    17761 aatccatggt gacgatggtc ttgaccatca cagggtcagc aggcattttt ccatgcttaa
    17821 ttctctgaga acagatgtag tccagaagta gcagaccagt ctcattacca gacaacagaa
    17881 catagtcacc atgtgcgctc ttaactgcga taccaacacg gtcgcagtca gggtcagtgg
    17941 ccaacagcag atccgcattg tacttcttgg catactccat acccagagac atagcttcct
    18001 taatttcagg gttcggataa ggacaagttg ggaaattacc gtccggcaga cgctgttctt
    18061 ccacaacggt cacattgctg tatcccatct ctgccagggt gcgggtaaca ggcaccagac
    18121 cggtaccatt cagaggagaa tacacgatag caacattctt gtccacttct tcgccaaaca
    18181 ggacggactg ccccttcact tcttcgatga aagcagtcag aacttccgac tgaatggatg
    18241 caatcttaga agcatcgcca tccatcttta catcagcaaa gatgtccagc ttttcgattt
    18301 ctgccagaat tgcagtagca gcctcggtag taatctgaca gccgtctgca ccgtatacct
    18361 tgtagccgtt atatttagaa gggttatgag atgccgtaat cataacaccg gcatctgcct
    18421 tcagataacg agtcgcaaaa gatactgtag gaacaggaag cagttctggc cacaagtgca
    18481 cctgaatacc atttgcagca aagatgtctg cggcagtctt tgcaaacaca tcagatttga
    18541 tacgggaatc atagccaacc accacggaag cggcttcata gttcttattc agatagtctg
    18601 ccagaccttg gcttgcctta cctacggtgt gtacgttcat gcgattagtg cctgcaccga
    18661 tggtaccacg cagaccacca gtaccgaaag acaaattttt atagaaagag tcctcaatgt
    18721 ccttctcaga ataggaacgc agttcttcta taatatctgc attggcatga ttcagccaac
    18781 gctcatattc tactttataa ttcatattat tatcctctca catccagaaa ctgaatcgac
    18841 ccgtgaatgc tgatttcagt agaatcggcc ggcacaaaaa ggcagtcccc cttatagatg
    18901 tccaacgatt cattatcaaa tcgaacggta ccacagccat tgacacacag caacacacgg
    18961 aagctgattg aatccgcacg atagtgtacc gtctgtcttc tctcagtgtt gacaatcata
    19021 cgagagacct cgaaatgctt gcatctgcaa agcaattccg cagctacacc agcacgatat
    19081 tttagaacac gaagcggctg tttcggttct gacaaccctt tcaagttcgc tacttccagc
    19141 gctttgtcta catgaagttc tctcttttta ccatttttgt caacacggtc ataatcgtag
    19201 agacgataag tcagattgga attttcctga atttctgcaa ttagtgcacc cgcaccaatc
    19261 gcatgaatcg ttcccgcctc aataaagaac acatcattct ttttgaccgg aaccttctga
    19321 agataacgct caacagttcc ctgagcaatc gcttcacgga tcacagcttc ttctgttatt
    19381 ttctgcagcc catagattaa gctggcatcc agtacatacc acatctctgt ctttccaagc
    19441 tgcccgtttt ctttttcaat tgcatattca tctgtgggat gtacctgaac agacaaatcc
    19501 tttttcgcat caatgaattt gatcaggatc ggcaattctc ctttggtctg cggatgcgtt
    19561 cccagaaact ctgggtgttc ttgcaacacc tccgtcaagg tcatcccctg atactctgac
    19621 cagacacaac aaagctcggt ccatccgggt gcgtagagca ttccaagttt ccgcaagagg
    19681 agacatctca attccttttg caaattcatc attcaggcgg ttccccccca cagataatcc
    19741 tttgcggaag gcttcaataa aaacggtttc tgtataacga gccctcctcc gttttttaat
    19801 actctaactc aaatttctgt ttatctttaa cattgatatc cttgccaagc tgtacttcaa
    19861 tcagcttcag ctcagtatct gccataatgg tatgccgaca gccagcactc atggtgatca
    19921 catcgccagc cttaacaacc tgctcatatc catccacgat ggtcttacca gtaccggcca
    19981 gaaccaccca cacctcgtca cggttcttgt ggctgtgata attcatggag tgactagcat
    20041 tcagagtaac cttaatggtc atgctctctg cttctacatc cataacctta taactacccc
    20101 aagacttctc tgcgaacatg atctgctgct cgaacttgtc aacgaaaggc ttaatatagc
    20161 tggactgttc cttatcggaa accagaatac cttcccgaga tgcacagatg accacatcat
    20221 gcagacccat agccaacaca ggaacatcca gttcgttcag gatgtgtacg ccatcgcaat
    20281 tctcattcat aacaccttta ccgataatgg attcctccat accttcggtc agagtattcc
    20341 aagtccccag atccttccac ataccagcaa aacgctgtac ctgaatcttg tcctcatgct
    20401 caacaacggc atagtcgaag gaaatcttag tcagagtatc gtatttgttg aacagatcct
    20461 gataatctac aaagtcaatc agctgatgtg cacgctccat gacatacttc agcttgtaga
    20521 caaacacacc accgttccac agtgcaccct gtgcgatata ttcctttgcc acagcctcag
    20581 aaggtttttc cttgaaggta tttacgaaag cagtctgctc tgcgctctca ggaatgatgt
    20641 agccgtactt ctcggaagga taagtcggtt caatacccat gagcaccaga ttagcttcgc
    20701 ctttgtcagc ctgatcggac agagatttca gggcctcaaa atagtcatct tctacgtaag
    20761 ggtcaaccgg gcaaacaact acggactctt ctggatcaat gcccatcaca tctaccagat
    20821 aagcggtagc caatgcgatt gccgggaagg tatcgcggcg acaaggctct acagatacac
    20881 ccacatcctc accaagttgg ttatggatag cagaaacctg tgtcttagag gttgcaatcg
    20941 taacagttgc gtcgttatct accttcttaa tctggcggta gacacgctga accatggatt
    21001 catatccatc ttcagttttg aaaatcttaa tgaactgctt ggaacggatg tcattagaca
    21061 gcggccaaag gcgcttacca gaaccgccag acagtaaaac aatattcata atgatctctc
    21121 ctttttataa gaaagaggct gctgcaatcc tcaaaaatca cagcaacctc ggtaaattag
    21181 cgttcagcac gcatcgggtt attcaggaag tcctcgtagg acagacggat gccatcttcc
    21241 aactcagtct tatatgtcca tcccagcttg gttgccttgg aaacatccag cagcttacga
    21301 ggtgtaccgt tcggcttgct ggtatcccac agaatctcgc cttcgtaacc aataaccttt
    21361 gctaccatct cggtcagttc cttgatagac agttccttgc cagtacctgc attaacagtc
    21421 tcgtcaccgc tatagttatt catcaggaac acacacaggt ttgccagatc gtcaacatat
    21481 aggaattcgc gcagaggaga accatcaccc cagcaggtaa cagtaggcag acctgcttcc
    21541 ttagcctcat ggaagcgacg aatcaaagcg gggagcacat gactgtgagt aggatgatag
    21601 ttgtcgttag gtccatacag gttagtaggc ataacagaga tatagtcagt accgtactgc
    21661 ttgtttagaa actcacagta cttcaggcca gaaatctttg ccagtgcata agcctcgttg
    21721 gtcttttcca gctcacttgt cagcagacag ctttccggca tcggctgcgg tgccatacgc
    21781 ggatagatac aggaagaacc gaggaactgt aacttcttac agccattctc ccatgcggag
    21841 ttgatgacgt tcatttccag aatcatgttt tcatacatga agtctgccag agcgttctgg
    21901 tttgcgataa taccgccaac ctttgcagca gcgagaaata cataatccgg cttttcctgt
    21961 gcgaaaaatg cttcaacagc gtcctgacgg cacaggtcaa gctccttatg ggtgcgggtg
    22021 ataatattgt tgtagtcctg acggttcagc tcgcgcacga ttgcggaacc agccatgcca
    22081 cgatgaccgg ctacgtagat ttttgcgtct ttttccatca taataatgct tgctcctttc
    22141 taaatttaga gcatctctca ttgcatgaga gaaatcaatt tgtctgcgat ttcgctcgca
    22201 gcatcataaa atgtatcaaa atttgaggtg tctagctgtt tgaggtcctc aatcacccgc
    22261 tctacgcggt ggaggttttc cggtgtgacg gaaattgcca gacccagttt ttgcaattta
    22321 caaatagttg ttctatcttc caaaactgta tcgcgggcga acagtgccat cggacgctgt
    22381 gcgagaatac actccgcaac agttccccat ccagcctttg taataacgta atctgccgcc
    22441 gctatatagt cctgtgtatt gggggtgctg ttagaaataa cggttacgtt ttctcccaca
    22501 agcggaacac ctagcgttgt aatgaaatca tacggcgcgt gactgacatc aatcggctgt
    22561 ttgaaccgcg cggacatacc gagtgcgaca aatacaggcg gtttttcatg ctgtgattta
    22621 atgcgttgca cttcgtcgtt atgaatcgga cgcgccacaa cggaagtcgg tgttttttcg
    22681 ctgttttgga gaaattccaa catttcttcg ttatttagcg cataaagcat ggtgtgctga
    22741 attttaccgt aacgcttggc atattctttc caaatatgtt cgggtaaaaa ttcgcgatac
    22801 agctctgtcc aagtgaagtt tcctacatac aggaaaggta ctcctgccat atcgcaggca
    22861 tccattgccc agattggcat atcgcaaagc actgcgctga cagcttctcg cttgagccat
    22921 tcggcttctg tctttgccaa agaaggaacc tttgccaaaa aatattcaca gatctcggtc
    22981 agcttacccg tgtccaccag tagggtgccc tcacggttaa tcagaccgat atctgtgttt
    23041 attggacgat agcgcacgcg gtctatcagc tctttgggca gttcctgctc caaattgcgc
    23101 tttgcaaaat ccagatgatt tgcgccgcaa accagtgtaa tcttcgcttc gcatcggcag
    23161 agtgttgcca taataatggg cagattccgc gcgatatgtc cgaagccgtg actggaaaga
    23221 tagaaacaga tatgtttatt caatgtcagc ttacttgact gcgttcttca ttgcagcttc
    23281 gcgctttgcc aattcacggt catgctttgc catgatgtta accagttcct cgtaagaggt
    23341 cttctgcgga ttccaaccga gaacggtctt tgccttagtc gggtcgcccc acaggttgtc
    23401 aacgtcagtc ggacggaacc actgtggatt tacacaaacg agcatcttgc cggtttcaac
    23461 atcgtagccc ttttcatcaa tgccagtgcc ttcccaacga atggtcatgc catttgctgc
    23521 gaatgccttc tcggtgaagt cacgaacagt gtgctgctcg ccggtagcga tcacgaagtc
    23581 ctcgggagtc tcatgctgga gaatcatcca catgcactca acatagtcct ttgcatagcc
    23641 ccagtcacgc agggagtcca tgttgcccag ttccagatgg tcctgcaggc cttctgcaat
    23701 gcggccggca gcgagggtga tcttacgggt gacgaaattc tcaccgcgac gttcggattc
    23761 atggttgaac agaataccgt tgacagcgaa catgccataa gcttcacgat attccttagt
    23821 gatccagaag ccatactgct ttgcaacagc ataagggctg taaggatgaa acggagtggt
    23881 ttcacgctga ggaacttctt caaccttgcc atacagctca gaagtagatg cctgataaat
    23941 cttagtcttc ttcgtcaggc ccagaatacg gcaagcttcc agaatacgca taacgcccaa
    24001 agcatcaaca tcgccggagt actcaggaac atcaaaagaa acctgaacat ggctctgtgc
    24061 agcgaggtta tagatttcat caggctgaac aatgctgatg atacgaatca gagaagatga
    24121 gtcggacagg tcaccgtcat gaagggtgat cttgcccatc aaatggtcaa tgcgagcggt
    24181 attggaaata gaagcacggc gagtgatacc gtgaacttcg tagcccttct ctagcaggaa
    24241 atctgccaga taggaaccgt cctgaccagt aataccagta atcagcgctt ttttcatgat
    24301 acattatcct ttcctatgta agtgataagg ccatataaat ggctcgtaat ttctaatact
    24361 tttcaagaat acacgataat tttcgatatt tcatagaaca atctacgcac gcattagcac
    24421 aatattagtt tttcgtg
//
LOCUS       DACGLW010000042        24269 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.042, whole genome shotgun sequence.
ACCESSION   DACGLW010000042 DACGLW010000000
VERSION     DACGLW010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24269
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.042"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            <1..570
                     /locus_tag="IXT53_001637"
     CDS             <1..570
                     /locus_tag="IXT53_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ4905461.1"
                     /translation="ASRIAIMSEGRVIQFDTPENILRHPANDFVEELIGEDRLLQAKP
                     NATRVGEVMLNTAITITPEKSLQEAIKLMREKRVDTLLVTDNSNVLKGFIDVETLNKK
                     RGKVSSVGDILNKHVFYVKESAYLRDTLQRILKRGLKYVPVVDEQNKVVGILTRASLV
                     DIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            574..1215
                     /locus_tag="IXT53_001638"
     CDS             574..1215
                     /locus_tag="IXT53_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ4905462.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            1212..2129
                     /locus_tag="IXT53_001639"
     CDS             1212..2129
                     /locus_tag="IXT53_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ4905463.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            2126..2797
                     /locus_tag="IXT53_001640"
     CDS             2126..2797
                     /locus_tag="IXT53_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ4905464.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(3338..3826)
                     /locus_tag="IXT53_001641"
     CDS             complement(3338..3826)
                     /locus_tag="IXT53_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HAQ4905465.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(4027..4293)
                     /locus_tag="IXT53_001642"
     CDS             complement(4027..4293)
                     /locus_tag="IXT53_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAQ4905466.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(4293..4556)
                     /locus_tag="IXT53_001643"
     CDS             complement(4293..4556)
                     /locus_tag="IXT53_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ4905467.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            complement(4549..4821)
                     /locus_tag="IXT53_001644"
     CDS             complement(4549..4821)
                     /locus_tag="IXT53_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905468.1"
                     /translation="MYWCCCIDSQALDSTFAKDDTLLPITLRLATASRKGVRSERGQR
                     SSLKRGLFLYVKKRKSLLNLSALLYFFDVLNQVRYNYIRKRRRDNG"
     gene            5032..5217
                     /locus_tag="IXT53_001645"
     CDS             5032..5217
                     /locus_tag="IXT53_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ4905469.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(5359..7182)
                     /locus_tag="IXT53_001646"
     CDS             complement(5359..7182)
                     /locus_tag="IXT53_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HAQ4905470.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(7780..10485)
                     /locus_tag="IXT53_001647"
     CDS             complement(7780..10485)
                     /locus_tag="IXT53_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HAQ4905471.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(10791..11738)
                     /locus_tag="IXT53_001648"
     CDS             complement(10791..11738)
                     /locus_tag="IXT53_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HAQ4905472.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            11928..12197
                     /gene="rpsN"
                     /locus_tag="IXT53_001649"
     CDS             11928..12197
                     /gene="rpsN"
                     /locus_tag="IXT53_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HAQ4905473.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(12373..12735)
                     /locus_tag="IXT53_001650"
     CDS             complement(12373..12735)
                     /locus_tag="IXT53_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ4905474.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(12746..13867)
                     /locus_tag="IXT53_001651"
     CDS             complement(12746..13867)
                     /locus_tag="IXT53_001651"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HAQ4905475.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(13883..14233)
                     /locus_tag="IXT53_001652"
     CDS             complement(13883..14233)
                     /locus_tag="IXT53_001652"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HAQ4905476.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(14363..15874)
                     /locus_tag="IXT53_001653"
     CDS             complement(14363..15874)
                     /locus_tag="IXT53_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HAQ4905477.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(16196..17548)
                     /locus_tag="IXT53_001654"
     CDS             complement(16196..17548)
                     /locus_tag="IXT53_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HAQ4905478.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYLEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(17701..18777)
                     /locus_tag="IXT53_001655"
     CDS             complement(17701..18777)
                     /locus_tag="IXT53_001655"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HAQ4905479.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            18973..20718
                     /locus_tag="IXT53_001656"
     CDS             18973..20718
                     /locus_tag="IXT53_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HAQ4905480.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(20998..21435)
                     /locus_tag="IXT53_001657"
     CDS             complement(20998..21435)
                     /locus_tag="IXT53_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HAQ4905481.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(21419..22792)
                     /locus_tag="IXT53_001658"
     CDS             complement(21419..22792)
                     /locus_tag="IXT53_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905482.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(23114..23602)
                     /locus_tag="IXT53_001659"
     CDS             complement(23114..23602)
                     /locus_tag="IXT53_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ4905483.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(23624..24226)
                     /locus_tag="IXT53_001660"
     CDS             complement(23624..24226)
                     /locus_tag="IXT53_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HAQ4905484.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         6957 a         5166 c         4487 g         7659 t
ORIGIN      
        1 gcaagccgta tcgccatcat gagtgaaggc cgtgtgatcc aatttgatac accagaaaat
       61 attttacgtc atcctgcaaa cgatttcgta gaagagttga ttggagaaga tcgcctacta
      121 caagccaagc caaatgcgac aagagtcggc gaagttatgc taaatacagc aatcacgatt
      181 acaccggaaa aatcattaca agaagcaatc aaactgatga gggaaaaacg tgtagatacg
      241 ttgttagtta ccgataattc gaatgtatta aaaggattta tcgatgtaga aacgttgaat
      301 aaaaaacgag gaaaagtatc cagtgtcggc gacattttga ataaacatgt tttttacgta
      361 aaagaatccg cctatcttcg tgataccttg caacgcattt taaaacgcgg gctgaaatac
      421 gtgccggtcg ttgatgaaca gaacaaagtc gtaggtatcc tgactcgtgc atcattagtg
      481 gatatcgtct acgatgtgat ttggggagaa gatcccgcaa ctactgatgt tgcagagtca
      541 gctactcctg aagaatctaa ggaggtctga cctatgcagc agttttttca tcagcacggt
      601 tcagaaattc tctcaaaagg ttttgaacat ctttatattt caggacttgc tttgatcatt
      661 ggtatcatca ttgctgtacc gcttggtgtc ttactgacac gttttccaaa agctgccacg
      721 attgtgattg gcttaaccag tgccttgcag acaatccctt ctcttgcttt attggcattg
      781 atgatcccgc tatttggcgt aggtaaatta cctgcgatta tcgcactctt tatttattca
      841 ttattgccga ttttacgtaa tacgtacatt gggatgaaaa atgtcgattg gaattatcgg
      901 gatgtggcaa aaggcatggg gatgactgag ttccaatcca ttttttccgt tgaattgccc
      961 attgctatgc caacgatcat ggctggtatt cgtttagctg ccgtttacgt gattgcatgg
     1021 gctacacttg cctcttatat cggcgctggt ggtttaggcg atttgatttt tagtggactg
     1081 aataattacc aaccagatct cattttggca ggtacgatcc ctgtaacaat tttagcacta
     1141 ttggctgatt ggcttctagg tctcttggaa caccggctga caccgatcgc acttagagag
     1201 gagaatgaag aatgaaacgt atactaaaaa tgaccatggt tcttttctct cttcttatac
     1261 ttgcgagttg ttccttccct ggtcttgcca gcaatacaga tgaagacacc atttcgatca
     1321 caggtggaat cacttcagaa gcacagatct tagcaagtct agttgccgga atggttgaac
     1381 attatacaga taaaaatacc gcaatcatca acaatctggc tactacaact atcaaccatc
     1441 aagcgatgat gaacggagat gcttcaattt cagcggctcg ctacacagga acagatttaa
     1501 cgactacatt gaaccttcct cctgaaaaag atccgaaaaa agcgtttgct accgtgaaag
     1561 atgaatttga gaaaagatac ggtcaaacgt ggttcccgtc ctatggtttt gaaaatacgt
     1621 atgtctttct tgttcgaaaa gacaccgcac aaaaatacca tctatctaaa gtcagcgatc
     1681 tgaaaaatgt agcggatgaa ttagtagctg gtgtagatac ttcttggatc aatcgtaaag
     1741 gagacggtta tgacggcttc caagaaactt atggcttctc cttcaattcg atattaccta
     1801 tgcagattgg acttgtttat gacgcagtcg aagcaggaaa aatggatatt gttcttggct
     1861 actctacgga tggacggatc gccagttatg atctagtcat gttggaagat gatcgtcaat
     1921 tcttcccacc atatgatgct gctccagttg tagacaacaa gatattagct gatacgcctc
     1981 atttaaaaga agccttaaat aagttaggtc ataccatcag tacggaaaaa atgcaggaat
     2041 tgaactacga agcagataat aatctagtgg aaccatcagt agtagcacaa cgtttcttac
     2101 aagaaaacca ctattttgaa gggaagtgaa gcaattggat aaaatgaatg tctttcaaca
     2161 atttttctat tatttccagg aaaatggctc ctatatcttt gcacaattta tccgtcactt
     2221 cctgatttct atctatggtg tcctctttgc agcagttgtc gggattccgg ttgggatcat
     2281 gatttctagg agacgtaaat tagcaaactg ggtgattcgt ttggcgaata tcatccagac
     2341 gatcccttct ctagccatga tctccatttt aatcattgga ttaggcctgg gagtcaatgt
     2401 cgtgattgtt actgtatttt tgtattccct cttaccgatc atcaagaata cgtataccgg
     2461 gatgatccaa gtagacaaaa acatccttga tgtcggcaaa ggcatgggga tgactgctag
     2521 acaacgacta ttcatggtag agttaccact ctctgtttct gttatcatgg caggcataag
     2581 aaacgcgtta gttgttgcca tcgggataac cgcgatcggt gcattcgtcg gtgctggtgg
     2641 tcttggagat atcattatcc gaggaaccaa tgcaacagag ggcacatcga ttattttagc
     2701 aggtgcattg ccaactgcat taatggcgat catcacggac tggcttttag gtattttgga
     2761 aagacggcta gatccagcca gccgtacatc acgctgatgt tattgtaaaa aacaaaagct
     2821 tctgcgacca tttgttggtc gcaaaggctt ttttagttct gtcattttta aggcaataat
     2881 ccactctgat tcttccctcc ttatgggcaa agagttgctt ttcttagcta tttatccatt
     2941 ccgtatactc caacttcata ctgccaacgg gttcatatct gcatccaccg actaagtttt
     3001 tttcttatcc gctaaggcat tttgccaatc cattttccca tcactcgtca tggaattcat
     3061 accacatgat ctctaaatac tcgtctgctt gttctttgaa ttgggcatca attcttacat
     3121 cctttggcgt ggctttcatt taagcctttt ccatcagtcc tcgattgttc gatgccttca
     3181 gctgcatcat caaaactggt gactgccgta tcggtaactt cgatcatctt ttatacaatc
     3241 gggaacttgc catcctcatc cttggctaaa aacatatcta gccattccag cttctcgcca
     3301 tgggaggttt gcggttccta aaaccggtac acgattctta tggactgcat ccgtcgcaac
     3361 tagacttggc gatacgatga tccttcacca gaagagcacc cccgtaaacc aattgatcga
     3421 tatattgcca ataagaaaag acattggcgt aaaagatatc gatccctcgt gatgcattgc
     3481 cgttgatact gctattcatg gccgaaagcg ccacgacttt aacttcttcc gtttgagtga
     3541 cattcatgtt cggcaatgtc gctttggata cccgctctgc taaaggaacg acactggtat
     3601 tgtatttcgt atctgtatct tttccaaaag accatgccaa cgaatcctct gggaaccaat
     3661 aagacgactc cggttgattg tccattgctt ttttcaacat acttttggct gtttctcgat
     3721 aaacgacatt tccaattgtc ttcgcctcac tttggcgata atcgatggtc gttgccactg
     3781 ccaacactaa caacgatgct ccaattttac tgattttcct tgtcacactt cttcctcctt
     3841 gttttacata aacccgtcaa cattccattc ctaaactcaa acaatgaccc ttcaaaggtg
     3901 gagatatagg aacaaattaa agaaatcatg caataaatcc aagaaggttg gttttagaac
     3961 aaaaaaagag tctccttttt ggggagactc tttgttaact atctaaaaag gttggctctt
     4021 ctcacttcac tcataatgtg tccaggcaga ataaatttct acaactttat tttcctcatc
     4081 aactttgtaa accactcgat gctgtctatt cagcctgcgt gagtatctgc cttcatgtgt
     4141 aggtctcagc ttttcaaaag actgcgtggg catatatgga tcttccttca atgtctgaat
     4201 cacttcttca aattgtttct ttaaattcgt ttgcttgatt tttcttaaat ccactttcgc
     4261 agagtttttg attgcgactg tatagttact catcaaagag cttcccaatc aatatcatcg
     4321 atattcgtaa agctactgtc gtctttttcg cggtctcgta ctttatctaa agtacctgtt
     4381 tgctcaagaa aaagggtttc ttgaatcgct ctccagtctt ccagcccaat caatacagca
     4441 ttgtttccac tacgatcact aataatctcg atttcagtat gattttcatt tacctctttc
     4501 aataactgat aaaaattttt acgagcagtg ctaggattca tcgttgtatt agccattgtc
     4561 tctcctcctt tttctgatat aattatagcg tacttgatta agtacgtcaa aaaaatacaa
     4621 taaagcactt aaattcagca aacttttcct cttttttaca tagagaaaaa gacccctttt
     4681 tagggaggat ctttgtccac gctcacttct tactccttta cgactagcgg tagccaaacg
     4741 taaagtaatt ggtaacaacg tatcatcttt agcaaaggtc gaatccaaag cctgtgagtc
     4801 tatgcaacaa caccaataca cgaaggttcc tcgttgcagt gtgacttgat tgatatcttg
     4861 aataacctga ggatacgcac gaaagatttg taacggttat tcaaaactga ttttggccgt
     4921 caagtcttct actgcctcaa aaatgattgc ctctttcgtc tcctgtcatt catgttcact
     4981 atcgatttga tacttgctta gaccgttggg tttgtgaatc accaattaaa aatggcactg
     5041 agttatggta acaaaaaatg gtcacttgac gaggctgaga ttcctactta tgaagacaca
     5101 tggcacttaa gagtcgaggt aaaggaagaa gctgaaaata taggaggctg tacattattt
     5161 ggcaaaggat tcggaaatta tgatcaagac atcaaaatca aaatgtttta cagctaaata
     5221 ggaagaataa taacatttca tacgctgttc ccatctttaa tggcttatag agggcacctt
     5281 tagctgagaa taagacaaaa cagaccctct agcttcaatt aagagctaga gggtctgttt
     5341 ttgattttaa aacttggttt atttcttcaa atggtagcta taggtgctga caacgatatt
     5401 ttcacgccaa gacaatcgca atcttaaccg caaacttgtt tggttatgga gaatattctg
     5461 ccagcgtctg tttggtacaa attgaggaat caacacagta gttgtatagt tttgtttcgc
     5521 tgcattttta cttacgatat ctacataacg gataattgga ttttcaatcg agcgataaga
     5581 agagtggacg attgaaaaac ggacatctgg gaaatgcttt ttaaattctg cttcgatttc
     5641 tttttctttt tcgacatctt cgtctagcga aacatgcatc gcaacgacat aatcaccgat
     5701 cgaacgtgcg taattcaacg caccgatatt tacccgtgtc acattcccga ctaatacaag
     5761 aacggtattc ccgtcataat catgtagttt tgcatcattt tctaaacgaa gctgttctgc
     5821 tactttttta taatgatcat ggatcttata aaaaatgaac ataacgattg gcataatgat
     5881 gaagaaaggc caaatgtccc ctaaccgata gacaaacaaa atcgcaatga ttgcatatga
     5941 gatgaacgca cccgtaatat tggcaatcga tttgcttaac catctttttc cttctttttt
     6001 ccatttgacg accatccctg tttgagataa agcaaatgga atgaacacgc cgatagagta
     6061 taacggaatc aagcgttctg ttgagccatg gaaaataaac agcaatacga tcgaacctag
     6121 cgctagtgtg ataatcccat tggaatagcc tagtcgatcc ccacgatctt gatacatgtg
     6181 aggcatgaat ttatcttttg ccagattgta tgcaagaact ggaaaagcag aaaaaccggt
     6241 gttagcagca actgctaaaa tcagcgctgt agcaaattga aggacgtaat acaaaatacc
     6301 atgtccaaag acagctttac caatttgaga caaaacagtg acttcttttt caggaacgat
     6361 cccataccaa tagttgataa atgtgatccc cacgaagaag aaacctaaga tcaatgccat
     6421 catcgtcaat gtagcagcgg cattttttgc tcttggcttc ttaaagaatg gtacagcatt
     6481 actaatcgcc tccaccccag ttaaagagga agaaccagaa gaaaatgctc gtaaaatcaa
     6541 agcgatagaa acacccggca cagccgctcc tggaagtgca gtagcttgca atggcacagc
     6601 acctgtcacg atattatata aaccaacaac gatcagtaac gagatcacaa ggataaatgt
     6661 gtacaccgga aataatagga acgatgcaga ttccctcagc ccacgaagat tcagcatcat
     6721 cagtagcaaa acgattgtca cagaaatagc gacttgatga ccgtagagtg ctggcaccgc
     6781 tgatgtgatc gcttcagcac ctgctgagac agatactgct actgtcaaca tgtagtcgat
     6841 caataaagag ccaccggaaa tcaagccagc attctttcca agattctcac tgctgacgac
     6901 atatgctccc ccgccatgag gataagcatg gatgatttga cgatatgata gcgttaatga
     6961 aatcaataaa ataataacaa aagcggcaat gggtagcgaa taccagatgg ctgcagcaga
     7021 caaagcgact aaaacgacaa cgatttgttc cgtaccatat gcaatagatg ataacgcatc
     7081 agacgacaat aatgccaaag cagcaaatcg ggttaatttg tgctcatcat tttcagcaga
     7141 ttttaaaggc ttacctacta acaacctttt taagtaatcc aatttctaaa ctcctttaca
     7201 cacaatttta cttcaagttt tatagtcgaa aatttatagt aaacaagatg ttatagtacg
     7261 ctcactgacc ttaaaagtca ataaacttta agaaaataat tggaaaaaaa cagaaaaata
     7321 tcaagcgctt tttcaaacgc tttaatacag caaaaatcta acattttttt atgggctttt
     7381 tctttgaatt ctataataaa aggatagaac ttgacttaga aaatcaagtt ctatcctttt
     7441 tgatatctta acttttggaa gagagatgta aggctgcttt ctacccatca ttcttactac
     7501 tcgaagcata gcgcagttgt cacaacctct tgataaacgg tgtttaaaag cttgcatctg
     7561 cggtaataag cccaaccaaa ttaaaaacat aagaagctgt ttttatttgg tcgttcttat
     7621 tacttgatgc agagcagctt ttttacaacc tcttggtaca cggtgttcaa tcagctgacc
     7681 ctcggtatta gctcaaaaaa acggcaaaat atgaggaact atttttccat ttttttctct
     7741 aatactcagg tcaaaacact gatttcacaa cctctttttt taatgaaaaa tatggctctc
     7801 atgcgccgct tctttgaatt tcgtcatttt ttcttcgatg aacggataga ttgccgttcc
     7861 tacgattcca aagacaacaa acaaaccgat catgatccag attgctttcg tagcatttgg
     7921 ccaataaatt cctcctgcag attcccgtaa caagtttacc gcatgagtaa atggcaagaa
     7981 cggattgatc atttggaaga atttccctga gacttggatc ggatagttcc cgcctccgcc
     8041 agagatcgac agtactaaga tgataatagc gatcccttta ccgacattat cgaataatgc
     8101 agccaagaca tagacgatca tcatgaacgc taaggcgatc aagacagcaa atgacacgct
     8161 gtagaccgga ttttggacat ctactcctag taagaaaata ttccctaatg taacgatcaa
     8221 gctttgcgca atcgccattg tcaaaaatgt cagcatacga gcaacgaatt gttctcgttt
     8281 cgtaaaattg tttcggtctt tctttcctaa atagtattct gtcgtagcaa cacttgaaag
     8341 aagaacagcg ccgacccaca gacatagtgc agtataaaac ggggtgctag ctgaaccgtt
     8401 atttgcgatt ggataaagcg cattggtttg tagctcaaca ggttgtgtaa agaagtcact
     8461 ttcttttgtt gcgtctagtt tcaataattt gatgacttcg cctagatcga cttcttgttc
     8521 gccttttcgg atcgcgtctg ctgctttttg gataccagct ttgatatttg gccagtcatt
     8581 gttgatcaag tccgcagcag cgttcacagc tgtttcgact tctggcattt tttcatttac
     8641 tgttgtcaat gtaccggtga tttcattttt tagtgctggc caatcatttt ggataaaatc
     8701 agcggccaat cctaattttt gctgaatgat cggtaattca ttgttgtata ggtctgcccc
     8761 ttcattgatg ccattgacga tggtatccat atttccattc agcattgtat tggcatcatg
     8821 gatttcttgt ccaattgcag gcatttcttt ttgatatttt tctaaaattg ccactgcatt
     8881 cgttacagtt gctttcgttg agctgagaag cgaatcaaag tcgatctggt ccgctttgct
     8941 cagtgcgcct tgtgcagtcc caatcgtatc gatgacttgg tctagtagag aagtgacagt
     9001 ggaactgatt tgacctgtat tgataccagc aatgatgcca ttgatattat taacaacggc
     9061 atcaatttga tctagcaatt catcaatagc cttcgtgtct tcattttgaa cagctgtgtt
     9121 caaagcagat aggcggtttt gcagattgct tagcaagggt tttaattgat ttagctgatc
     9181 aataattggc tgtaatttcg tatttccagc acttgtttgc aatttagtca ggaactcgac
     9241 cagtttatcg attgcatttt gttgttgaga tattccgtca ataaagccgt tcaatacatt
     9301 gctgatgttt ttcttttcat catcagtcag cttaccatct tcaatagctg ttcttacagt
     9361 atctattaac gaacctgtcg ttgttgccac ttgtccaatt gacttcaatg taacatctac
     9421 actgtttgtg atgcttggta tcgcatcttt cagctgagag gcacctgatt ttgtttggtt
     9481 tgctagatca cttgcctgat tttctaaatt tttcacatct ggcaagatat cttgtacttg
     9541 ttggatgatc gtcaagcctt gttttgcttc atcgattcct tggttcattg tctcttggat
     9601 cgaagcaaaa tcgccgtcga tctctgctag ttgcttaccg gcattttgga tctctgggat
     9661 tttttcttgc aaatcaagga tgatttttgc ttgatctttc aactcaggca ttttgtcatt
     9721 caatgcaacg actttttcac ccatctcatc tactttaggg atgtaatcca cgaattcatt
     9781 tgctttattc agtttttcct tgatttcagg tagttgactt tgtaattcga ggacttgttt
     9841 tgtgtagccg tcgatcgtgt ccaaattttc atctgttgat aaaatcatat ctttaacttt
     9901 attgatactt actaggttcg tatcgatgtc gtaacctatc tcattgaata ctttcaataa
     9961 agtcgaactg gctgttttga taaattcatt tgtgatttgc gattggatcg aacttgctcc
    10021 tttatcggtg attttcggtg caatcgcatt gattttttca tttaccgtat attcgatttt
    10081 tggtttcttg atatcaccac tagtaaaact cagcagatct tctgagaaat cctttggcaa
    10141 gtaaatacct gcgtaatact ttccggaacg gactccgtct tctaattgtt cttttgaatc
    10201 aacgaattgc catcctaatt gtttattttt atgaagcgat tcgatgactt gctccccaat
    10261 ggccacttct ttaccttgaa actctgctgg cttgtctgca ctgtaaacag caattggcag
    10321 ctctccagta tttccatatg gatcccataa tgctttgatg ttaaaccacg catatagcga
    10381 agggatgatc atgatcgcga tgatcagaag tgttgcaatc gggtttttga aaatccgttt
    10441 ccagtcaagc aaatataatc gaagcgtatt tttgattgac tgcatagttc tcccccttct
    10501 tcttttttca gctgaattac tttaccatgt atttcatact tttttcaatc ggcttttttc
    10561 aaaaaagtcg aatttttgga catatcacgg caattgtctc attgtaatct cttacatatt
    10621 gagaaataat taaggattac agcctttact ttatcatgaa ataaaaggga aagataattt
    10681 tatgccaaag tttttatcta ttatacgtat acgcaaaaaa gctaaaacat tttttgtctt
    10741 tgcccctaca aaaatgtttc agcttttttc gttttttatt cctgtatttc ttatttttcg
    10801 aatgacagga tgattttccc agcaggagga tgtccttcaa ctagctgatg tgcttggatg
    10861 acatgcttaa gtgaagcaga cagattcatt gaaataaaaa catgaaaggc tcctttttgg
    10921 taggctttga ttatgccagc aaaggcttct gcatctagat attcctttct tggaacaaac
    10981 gattcataaa agccttcctt tttttgccgc aaatctagat ctgggaggtc atttaatgca
    11041 acatacctgc cgcctggttt catgatctgg atgccggttt ctcctttgat acttcctttc
    11101 gtcgcatcga ttacaaggtc tgcctgatca gcaaattgca gacctggatt tgttttatca
    11161 taagcagcaa atgctgaagc tcctaatttt tttacctttt cctcattttt actggatgca
    11221 ctggttaaga ttcggattcc cttttcatgg agaagctgga tcagacttga cccgaccgca
    11281 cctgatgctc ctagtatcat tacagtatct gtcggctgga tttccgtcaa atggttgatt
    11341 agattataag ccgtaattcc tggcgtgacc attccggctg cttcttccca actcatttta
    11401 tctggaattt ttgctacttt tgctgatggc aacacgactt cttctccgta agtcccgccg
    11461 actgcatgga cagctactcg atcacctaca tgaacagttg tgacatctga accaatctct
    11521 gtcacaatac cagcgccatc attacctggt acataaggga atttcaatgt tcgtatctct
    11581 ttcatttctc ctaaacgcaa ggcaacatca taaggattca cactgaatgc ttttatttct
    11641 acacgaacat gtccagaacc gacttctctg ggatgagcat cgatttcttc aaaaacatct
    11701 tgtgcttctc cgtaaccgtt aattgcaaat cttcgcatgg tcaccatctc cctttttctt
    11761 taagtttacg tttatgcctt tattttgaca aaaaaaatgt cttttcgttt tggcgcatga
    11821 ttcaaaacaa aatagttgtc ttttttcgcg taacgtgtta aattatttat ctaaaagcgt
    11881 aaagattacg atttacgcta ataaaaaata attggaggaa tttgttgatg gctaaaaaat
    11941 caaagattgc caaagcaaaa aaacagcaag aaacgattga acgctatgct gctttacgcc
    12001 aggaattaaa agcgcaaaaa gattacgaag gcttgcgtaa acttccttta gatgctcgtc
    12061 cagaacgatt gaaaaatcgt gatttaatcg atggacgccc tcgcggatac atgcgtaaat
    12121 tcgggatgtc ccgtataaaa ttccgtgaat tagcacataa aggtttgatt ccaggagtaa
    12181 aaaaagccag ctggtaatcc aagtctggct gtaatgtaaa ggaggagggc gcatggacca
    12241 ctccttggat catcaaaaaa gatacgttcg cttcttctag cagaaatgct ggtactagaa
    12301 gcagacgtat ctttttttac gcacagctgg tcctttttta tatgtaaagg ctgcatggtt
    12361 acttcgctat atttatttag taacaggagt cagtgcttcg atacccacac taattttcaa
    12421 tgctcggtca acttctgcca taatgggtcg atccaaatgg cagacctttt ctttcaatct
    12481 tgatttatcg atcgttctga tctgctccaa caggataacg gagtctcttt ctattcctgt
    12541 tccagtagct ttgatcccaa tgtgagtcgg aagcttaggt ttagccattt ttgccgttat
    12601 ggccgcaaca atgatcgtcg ggctaaaatg gttacctaga ttattttgaa taaccaaaac
    12661 tgggcgtgtt ccgccttgct ccgatcccac gactggagaa agatcagcaa aataaatatc
    12721 tcctcttttt accataggat cctccttatt cttgatattc tcttggtatc cgctgtccta
    12781 atccgcaagc cacttcatag tggatcgtac ctaattgatc tgctatatcc tgcatagtga
    12841 tttctttgtc tgcatttttg ccgataagcg tcacttttgt tccgacagga aattcttgag
    12901 gcaatcggat catcagttga tccatacata cacgcccaat cgtttcacag tagttccctt
    12961 ctaccaatag agaaaaaccc tgcatctttc gtggccagcc atctgcataa ccgatcggta
    13021 tcgttccgat ccactctgct tcaggagtga tatatgtttc tccatagccg attccttcac
    13081 ctgctgggag ttttttgact tgaatcaatt cagaaaccag ctcaagtgct ggctgcaatg
    13141 gataaacttc cggtagtgcg tgcccagagg gattaagtcc gtacattgct actccataac
    13201 gaatcatatt gccgattgtt tcatcatgcc ataaagccgt tgcactattg ctgacatgta
    13261 catatcgagg aagttcggga agttttttca gcacttctat aaagcgttct ttttgcagat
    13321 tccaatatgt atcatctgcc tgatctgctg tagaaaaatg ggtaaataaa ccttcccatt
    13381 ctaatacacg actttctttg atgaatgcag ctgcctcttt tacttcttca ggtgtacaaa
    13441 atccgatacg acccatccca gtgtctgctt ttagatgaat cgaaagaggc gtctctagct
    13501 tacttttctc taaaacagca gctgctgctt gcaaccattc ccgtgtacct gcagttactg
    13561 ataaatcatg agcaacagct acagtcagtg cgtcgtaagg aaccatattc aagataagga
    13621 tgggctgagt aaatccagct tctctcaact cgactccttc atctaaattt gaaacacaaa
    13681 agcccgaagc tccacctgca actgctgcct cagctgtttc aatagctcca tgaccgtatc
    13741 cgttcgcttt tactactgca aacaattctt ttccttgcgg aagtcttgca acttcatttg
    13801 ctacattttc agttatagct tttttatgta tcactgcttt cgttgggcgg tgccaagcga
    13861 caaccatcgt ttttccttct ttctagtctt ccaaaataat ttgtgcaaaa gctgtttcat
    13921 ttgtatgagt aatactgacc catacattgc cttcatgagg tgaacgcgtc acaacaggct
    13981 gaccattttc attttttaaa atctcgacat cttgaaaggt aacttttccg atcccagttc
    14041 cccaagcttt agagaaagcc tctttgcaag cgtaacgccc gcctaaaaat tctacttggc
    14101 gatgaaaagg caaggactgg aatagtttca tttcatcaga ggtcaagatt cgtgcaatga
    14161 attttggttt ttcttcgatc agccgagtga tcctaggaag ttcgactgca tcgatcccaa
    14221 ttccttttat catatgcttt tctcttttca ataaatttag aacactctca ttctaccaaa
    14281 ttataaccat tttgccacaa ataagtccac aaataacaaa aatgaaagtt tctcttaaaa
    14341 aagagaaact ttcattttat gtttaatcgt tatttgaacg gattacaaat ccgcgttttt
    14401 tatcattgtg acgtttaccg ccatcttttt ggctgttatt atttttgcta taaccgcctt
    14461 ttgatttgtt tccacgataa ttaccgccgt cacgacgatt acggttgttt ccgccaccac
    14521 gtttgccatt tttattgtat ccttttttct gcattggcaa tgggcgttct ggtgtgatct
    14581 tcactggcac tgcatcagct ggatcttttg ctgtcgtttt tagcaaaagt gcaactaaat
    14641 cttgtgcaga atattcttct aataatttat ctgctgtttg caggtattta tctagaccat
    14701 tttctgcaag ttttgtttcg atttgttcaa ctgcagcacc taattgacct ttgaatgctt
    14761 ctttttcggt tggtggacgt aaagtcgtca tccgtttttt cgtcaagttt tcaatcacat
    14821 gcaagtagtc catttcattt ggtgtaacga aagtcacaga cataccgcct tttccggcac
    14881 ggcctgtacg tccgatacgg tgaacgtagc tttctggatc ttgagggata tcatagttgt
    14941 acacatgagt gacacctgaa atatctaatc cacgtgcagc aacgtctgtc gctacaagga
    15001 tatctaaatg accgctctta aatgaacgca atacgctcat tcgtttttgt tgagacaaat
    15061 cgccatggat tccttctgca cggtaaccgc gtgcttctaa tccgcgtgcc aattcgtcta
    15121 cacggcgttt tgtacgtccg aagacgattg ttaattcagg tgtttgaaca tcaaacaagc
    15181 gagtcatgat atcgaatttt tcgtattctt ttgcgcggac atagtattga tcgatcaagt
    15241 cagctgtcat ttctttcgct ttgatcttca catgatgtgg atttttcatg aatttaacgc
    15301 caatattctt gatagctggc ggcatcgttg ctgagaaaag taatgtttgt ctttgatcag
    15361 gaacttgtga aatgattttt tcgatatctt ccaagaagcc catattcaac atttcatctg
    15421 cttcatccaa aactaatgtt tgaacggttc caagtttcaa tgtgtggcgg ttaatgtggt
    15481 caagcatacg tcctggtgta cccacaacga tatgtggacg atctttcaag ccacggatct
    15541 gacggccaat atctgctccg ccgtaaacgg cttgtacacg tacttttttg tcgcgcccta
    15601 aacgatataa ttcttcttgt gtttggatag ctaattcacg tgttggtgcg ataactaaac
    15661 cttgtaattc gtgacgatct ggatcgattt tttctaacat tggtaatcca aatgcagctg
    15721 ttttccctgt acctgtctgt gcttgtccaa taacgtcttt tccagctaat gctagtggaa
    15781 tcgtttctgc ttggataggc gttgcttctt caaaaccagc gcgttcgact gatttcaaca
    15841 attctggtga taattctagt tctttaaatt tcaaatgaat cctcctatat ggttgttaac
    15901 tgaggatgta gcttttttgc taccccgtcg atttgttcgg tcaaagtgta ttgtttcttt
    15961 ttgacagtgg ccacgcacgt ttttatccat tcttagcccc tgtggcacca ccgaaaaatc
    16021 actttgcgaa gtttaggcat tagaaaagcc aaacgggtcg cgttcagctt tatcattatt
    16081 atgacatctg gcttcaacaa gttgtttcta tggcaaaacc agtccaattt ctcttcatct
    16141 ttcggcaata ggttattcta acatagtttt tcgttttctg caagtttaca gatttttatt
    16201 tcatttgctg aagttgttct accacttcaa ataagcccat gctgttgctg ccttttagca
    16261 taatactatc tgttgcttca agattttgtt tgatagcatc aatcaattta ttcttttctt
    16321 tttccaaata gaacacaggt agttgggggt atttttggtt caattcttct tttagtgcat
    16381 gcatttgttc cccataaagg aaaatcatgg cgaatttctc aggatcgata tgttcactca
    16441 tttgcgcatg catatttaaa gactctttcc ctaattccag catatctgct aacacagcga
    16501 ttctgcgtcc ttttgttggc agatttttaa aagtatctaa taccagtccc atggctgttg
    16561 gatttgcatt gtagacatcg ctcaaaatag ctgcaccatt tcctgccgtt agccattcgg
    16621 ttctgttttg agtgatagaa acatgggcga gcccttggcg tatctcaggt atggacagtc
    16681 caaagaagcg cccgatcgta tacgcaatca aagcattcgt tacattataa cttccaggca
    16741 gcgggatcgt aaatgtttct ccttctacta caaaatcggt ttgtgctttt ccttttgctg
    16801 tcacttccgc atgaacatcg ccatttccaa taccgaatgt tgtgactgtt tgtactaatg
    16861 ttgccgttaa aggtgtcagc aacggttcat ctgcgggcac aacgagtaat ccgtctgata
    16921 caagtccgtc agtgatttcc attttggctt tagcgattcc ttctcgcgac ccaagatttt
    16981 ctatgtgtgc ttcaccgatc attgtgattg ctgcagcttt tggttgagcc ataagtgaaa
    17041 gttccgtgat ttcattggcg tgatccatgc ccatttccaa aacgatcttt tctgtatctt
    17101 ggggcatgtg caaaatcgtg taaggaagac caatattatt gttgtaattt ccttgcgtct
    17161 tatatgtttt gtatttttca gacaagacag acgcggttag atctttcgtg gtggttttac
    17221 cattgcttcc tgtaattgca acgacttctg cgcctacttt ttccaagtaa taggttgcca
    17281 attgctgcat ggctttcaaa ggatctgcta caggaataac tggaaaattt tttggagctt
    17341 gttcaacagg caagctccaa aatgaagcag atgctccgtt tgtgatcgct tgctggataa
    17401 attcatgtcc atctcgcgta ccagcaagag gaacgaataa acttccttcg gttatcttcc
    17461 ggctgtcaaa ttcgacattg gtaatctttg tcagtgcaga gcattcgctg cccacggctt
    17521 gtgcaatttc ttgtattgtt agattcattg agatctctcc taaaaaaggt tgtgaaaaaa
    17581 gcgtttggcc taaacggcgg gttcgcatga ctctcacttc caagtaaatt tctctattcg
    17641 tatttagaag ctagaatgaa cgcggttacg caatcactcc agcctcttct ttatttctct
    17701 ttattcattt ttttcaaaaa aagattgacg ctgatggtat cgattcattc ctaactggat
    17761 cagttcttca atcaaatcac cgtatttcaa gcccatattt tcccataaga gtgggtacat
    17821 actgaactcc gtaaatcctg gcatagagtt taattcattc aggaataatt catttttatt
    17881 tgtcaaaaag aaatcgcacc ggctcaatcc gcttccacct aacatcgtgt aagctaactt
    17941 cgcgtactct tgcgcttttt gataaacttc ttctggcact tcggctggaa tctgcatttc
    18001 gattttatta ttgatatatt ttgcttcata atcatagaat gctacgtctt ttacgacttc
    18061 accaggcaaa gtcgtccgaa cgtcttcatt tcctaataca gcaacttcga tttcgcgcgc
    18121 ttcaattcct tgttcaacga ttgctcgaga atcatactga taggctgttg ctaaagcatt
    18181 ttgcagctct tctcggtttt ctgcttttgt aatgccgaca ctagaaccca tattcgccgg
    18241 tttgacaaac atcggataaa gcaaagaacc ttcacattga tcaaatactt ttttaggatt
    18301 ttctttccat tgattcttaa gtactggtac ataaggaact tgcggcacac cagcagcttg
    18361 taaaatatac ttggtcatga ttttatccat ggcacatgca ctggtcaata cgcctgcgcc
    18421 gacataaggc atattcaatg tctctaagaa gccttggatc gttccatctt ccccgtttgg
    18481 cccatgtaaa actggaaaaa cgatggctcc ttcttctttg atttcgcccg gattgatcac
    18541 ttttcctgta aagccttcct ctgtctgtcc acttgggtcc catgaaagat gcaagacatc
    18601 tttgctggca ggtttttctg ttagtaatgg acctttgacc cattgtcctt cttttgtaat
    18661 aaatacgagt tgaacttggt aataattata ataaatggca tttaaaactg aaaatgcgga
    18721 aagaatggac acttcatgct ctgcgctgcg tccgccatat agtaaagtaa tcttcaaaag
    18781 attgtcctcc tgtattctaa atcgttcagt gatttactct catattttag cacaaaacaa
    18841 aaataaacga ataacttatt tataaaaaaa cgagccagct tctaaaagaa cgagcgtcat
    18901 ttattttttg tcagctagat tatttgtccc gttttttata aaaagttatg ataaagaaaa
    18961 ggagggatga tgatggcgca aacaaatcca cgcagccgct tggcagaaat cattcgagtg
    19021 ttcatcaatt acaatgtagt gccaaacttt gtacagcaaa aaaatcccga acaggtaaaa
    19081 aaagcctttg aagaactagg ccccaccttc atcaagatag gacagatgct ttctgttcga
    19141 gaagatttgc tttcttctgc cttcactcaa acattcaaaa cattacaaga tagtgtacct
    19201 agcgacacat tttcaacggt caaaaagacg atcgaaacag aactttctct ttcattatca
    19261 gatatattcg atgatttttc aaaatcgcct tttgcctctg cttctatggg gcaggctcat
    19321 cgtgccaagc tgaagaacgg tgacgcggta gtcgttaaga tccaacaccc taacatcgcc
    19381 gaagagatcc gacttgatct tcaactattt gaacgagcga tccctttgat caaatacatt
    19441 cctgaaacta gcgtagttga tctaaaaggg gtactccaag aagtaaaacg ttccttgatc
    19501 aatgaaatgg acttcttaaa agaaagtcaa aatggcgaac aattctatca aaagaacaat
    19561 ggctggaaag agatccgcag ccccaagatt tatgacgctt tttgttcgaa aaaagtgatc
    19621 gtgatggaag aaatgtctgg taaaaacttg aatcatttaa tgaacgccga aaacaaaact
    19681 gaaactttta tcactggaat tcaaaacaag cagctgaaac aagaagttgc aaagctttta
    19741 gttgagaact ttatgaaaca ggtgtttgat gatggttttt tccatgctga tcctcatcca
    19801 gggaatctgc tattccatgt actaacaaaa gaagaacaga cacaagctag ccgaaaaact
    19861 gaaacagtcc atgaaaaaga gttcggttcc tttgcttttc gagcctccac ttctgcagaa
    19921 gaccctgttg cgccatatac catcaactac atcgattttg gtatgatggg gcatctttct
    19981 gcaggactta ggcaaaaact cacacaagcc gttttagcgc tgtatacgaa agatgcttat
    20041 cgaatcgaaa aagcggtcct tcgactttgt cagcaagaag gctcgtttga tgaaagtcgg
    20101 ttccaccaag aactgacaag ttttcttgaa cagtattatg attctcctat tgatgaaatc
    20161 aacttgcaag aagtattcac acatgtcgtg acgatttgtc atcaaaataa tctgcagttc
    20221 gatcgtgata tcacgctttt gctgaaagca tttgggacat tagaaggggt gatccgcgtc
    20281 ctcgatcctg aagtttccct catggaagtc gcttcgcctt ttgctcaaca ttattttctc
    20341 actcatctgg atgtagaaga tacattgaaa caaagcggat tagacttatt ggaagggatg
    20401 aaggctgcgc ctaagatccc tcaacagctc caccatctat tagaaatgtg gacttcggga
    20461 caaggaaaag tcaatttgga attgaaaaag caagacaaat tattgagcag gatcgaaagt
    20521 atgatcaatc ggcttgtttt cggtatgatt ttagctgcat tgatcgtagg gtcctccctg
    20581 cttgtccaag cagcacctgt agaaaacgca gaggtcgttt ccttattagg gatatttacg
    20641 tatgctattg ccgcattcgt cattattttt ttggcaatcg atgcattgat ccaaatgtat
    20701 aagaagagga aaaagtagaa aaaggttgtg acagaaagaa tcagcttcaa gaaataagaa
    20761 ggggttttac ataaattgtt tctcaaattt ttgtgaaaat tcggcttatt tttcgaagag
    20821 gctacttctg aaacaccgag aataaaaaat gtgaaaaagc tgttctgcac ccagtaataa
    20881 gaacaatcaa aaagggaaca tggaacacat ttatcctgtt tccctgttcc ctttttgatg
    20941 tattcgagca taaataaaat accttcgtta cctactatct tctgtacttg gtaaacctta
    21001 gtcaattgcc ttttttaacg cttgacgaaa agacatatga tctttctccg taaagggttt
    21061 tggacccttt gtccttatgc cagcttttct taactttcca ctatgataac gttgttctag
    21121 aagcgtgccg atattcgctt cgtcaaattc ttttccagag tgatggaaca ctcgcacacc
    21181 cttcggcaat acaagtgaag ctaaaccata atcttgcgtg atcaaaaagt cattcttctt
    21241 aaccataccg acgattttat aatccgcact atcagctccg cggtctacgt agacgatttg
    21301 gacatgatta gcgtattctt tggtcgtata atggtccaca ctggtcacga taaccacgtc
    21361 aagaccatag gattctccta attttatgac atcttctttt actggagaac catctccatc
    21421 aatgaatagc ctcattcttt ttcgccttct tccataaatg ataaacagtt actaatccgc
    21481 tcagtaaaag accaacattg atccattttg ccaagataag cagttgtaca ctttctatcg
    21541 gcactgattc atggttcagc gtgatcaata atagatggat tggctctccc atttcttttg
    21601 ctaaaacaaa caaaagcgtc atcaataaaa actggtggat cacactccct ttattttgag
    21661 tagtatactt gacaacgatc cggcgatcca ttggaatagc tgtatcagtt tgataatatt
    21721 ccttatttag ccagctgcct gttactacgt gccagaaact aagcagaaag acacctatcg
    21781 caaaaccatt tggaatcaag aggaccaata aagaagaact cagtccaagc aattgcatag
    21841 ccaatggaga ccatgttaaa cgtttcaaaa tccatggtcc gactattttt gctgcaatca
    21901 agcctgctgc ataaggcaaa tacataaaaa tcgaaagttt ttctaatccg tacagtatgc
    21961 caacataaaa actgctaaat aaaaaaagga aattccctag cattccattt agaaaagtaa
    22021 acagatttag ccagcttggc aacttccatt ccttcttctg gtgtcctaaa acaaacacga
    22081 acaagatgaa cagcagcaag aaaccataaa tagcaagatc aaaaagcctt tcatcgaata
    22141 acaatcgggc atttctcaaa agaaacaata agccaaagaa tatcaaaaat aaaacaagct
    22201 ctcgcgtagc taccagattt cttttgatgt ctttgaaatc cagttcgtaa cgcggataat
    22261 gactaaccgt atggtaggcc atgacgtaaa aaagtgtata aatagtaaag cttgctgcaa
    22321 tctgagtcgg tgcaggcaac tcaatcgctt gatacaggag aaatagccag atagctgcaa
    22381 ataagtactt tttcccatcc atattgataa aaccttgcgt cttttcgtga aaattgaccg
    22441 tggtattagc aggtacaagc catgctgcac tcagccctag caaaacactt gaaaaataaa
    22501 ataaaggaaa ataaaatacg cctaatattc caagcaatga accagcacca ccaatcagca
    22561 gagcgatcat cagcaacgtg taactatcta gaccaaatcg tagactgttg actaaaaata
    22621 gtccagtcat ccgaaaggca taaaacagcg taaatggcaa aatcgtcagc caattatgcg
    22681 agtctgttag tcccaaaaat aaaagataag gaataaaaat aaccgtgtta cttaagataa
    22741 agggcgttgt ttgtgtaaaa ttacgcccaa atcgtttgag tttttcgaac atgacaatgg
    22801 ctccttaaac agtcttataa ctaatctgct cttcgatttt agcattaaag gggaaataaa
    22861 ccaattttac agaatatatt cttgtaaaaa aaaatacaga aagaagcata agcttctttc
    22921 tgcattttta cggtacttga ttactcaagc agacaagccc ttctccacaa aagtcttgcc
    22981 cgacacccct gcatccggca ggtgataaag atagaaatca attatcctgt gcctccaaaa
    23041 tcaattagga gctctgtaaa ccagagtcct tctcgacccg tcgcataaat ttactttaac
    23101 acagttccta tgtttatgcg agagcttttt tcattttata atcgaccatt tgaaatcctt
    23161 gaccgatatc tgaaataagt tcttctgtaa tctcaaatcc ttcatgctta taaacctcga
    23221 ttgcttgatg attcccctga ttcactcgca aaaagatctc tctttccttt gtttgcgcag
    23281 caagttcttc ataccagcga aaaactgaag aagtcaatcc ttgaccacgt actttattta
    23341 gcaaatataa tttacttaaa taaatttttc catttatctc ttcataagca gtgtaaccga
    23401 cagtcttttc ttcgcttttt aataaaaagt aggacacacc ttctgtcagt tctttttgaa
    23461 tctgtttttt tgactgataa gttttcagca tgtaatccac ttgtttttga ccaatgatcg
    23521 gcacaaaagc ttccggccat acttcttgta tgactgccat cagctcatcc agttcatttt
    23581 ctgttacttt tattttttcc atacacccac gtcctttttt acattataaa tgaccttgcg
    23641 cttgatggaa taaaaagagc atgatcatgc tatacacacc caccgaccat ggtttcaaga
    23701 aaatcacaat ctgcataaat tccctaaaag ataatttcgt caaaccagct actagacaga
    23761 tcaagtcgtc tggcgcaaag ggaagcagca aggtaacaac tagtactttt ttaatatctt
    23821 tcgtttttct tgtcagcaat tcatcaaatt tctgatactt ttttggcgat aaaataagct
    23881 ggacaaaggc tacgccatag taacggacaa gcaaaaatcc gattgcttcc ccaatcacta
    23941 ggccaataca actatataaa agcccaaatc cattgccaaa catcagcatg cccgctactg
    24001 aggagatccc tcccggcaaa ataggaatga tcacttggat cacttgcaac agaacaaata
    24061 tcatcactgc atacttgcca aattgctgga taaacgcttg caaagacgtg ctcgatcgaa
    24121 aaatcccatg atgataagca taaagaatca acacaaagaa aaaaaacaga ccaataatgg
    24181 gcatgatctg aagacatttt tgtaaaactg atcctttttt agacatctat attccccaca
    24241 tcttttttca tttattaaaa ctgcctcct
//
LOCUS       DACGLW010000043        23405 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.043, whole genome shotgun sequence.
ACCESSION   DACGLW010000043 DACGLW010000000
VERSION     DACGLW010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23405
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.043"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            complement(51..1778)
                     /gene="ptsP"
                     /locus_tag="IXT53_001661"
     CDS             complement(51..1778)
                     /gene="ptsP"
                     /locus_tag="IXT53_001661"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HAQ4905485.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDTMEEV
                     VELVHEYVK"
     gene            complement(1778..2044)
                     /locus_tag="IXT53_001662"
     CDS             complement(1778..2044)
                     /locus_tag="IXT53_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HAQ4905486.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            2523..4757
                     /locus_tag="IXT53_001663"
     CDS             2523..4757
                     /locus_tag="IXT53_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAQ4905487.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            4905..5225
                     /locus_tag="IXT53_001664"
     CDS             4905..5225
                     /locus_tag="IXT53_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HAQ4905488.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(5430..5654)
                     /locus_tag="IXT53_001665"
     CDS             complement(5430..5654)
                     /locus_tag="IXT53_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HAQ4905489.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(5852..7432)
                     /locus_tag="IXT53_001666"
     CDS             complement(5852..7432)
                     /locus_tag="IXT53_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HAQ4905490.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(7833..9209)
                     /locus_tag="IXT53_001667"
     CDS             complement(7833..9209)
                     /locus_tag="IXT53_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010767236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAQ4905491.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(9337..10455)
                     /locus_tag="IXT53_001668"
     CDS             complement(9337..10455)
                     /locus_tag="IXT53_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ4905492.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            10597..11031
                     /locus_tag="IXT53_001669"
     CDS             10597..11031
                     /locus_tag="IXT53_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ4905493.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
     gene            complement(11109..11642)
                     /locus_tag="IXT53_001670"
     CDS             complement(11109..11642)
                     /locus_tag="IXT53_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HAQ4905494.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(11736..12914)
                     /gene="mutY"
                     /locus_tag="IXT53_001671"
     CDS             complement(11736..12914)
                     /gene="mutY"
                     /locus_tag="IXT53_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HAQ4905495.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(12907..13704)
                     /gene="recX"
                     /locus_tag="IXT53_001672"
     CDS             complement(12907..13704)
                     /gene="recX"
                     /locus_tag="IXT53_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HAQ4905496.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            13811..15193
                     /gene="rlmD"
                     /locus_tag="IXT53_001673"
     CDS             13811..15193
                     /gene="rlmD"
                     /locus_tag="IXT53_001673"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HAQ4905497.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(15367..15945)
                     /locus_tag="IXT53_001674"
     CDS             complement(15367..15945)
                     /locus_tag="IXT53_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin-binding protein"
                     /protein_id="HAQ4905498.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            16267..16737
                     /locus_tag="IXT53_001675"
     CDS             16267..16737
                     /locus_tag="IXT53_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HAQ4905499.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(16845..18071)
                     /locus_tag="IXT53_001676"
     CDS             complement(16845..18071)
                     /locus_tag="IXT53_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR family transcriptional regulator"
                     /protein_id="HAQ4905500.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(18825..19025)
                     /locus_tag="IXT53_001677"
     CDS             complement(18825..19025)
                     /locus_tag="IXT53_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ4905501.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(19159..19767)
                     /locus_tag="IXT53_001678"
     CDS             complement(19159..19767)
                     /locus_tag="IXT53_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HAQ4905502.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNAAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(19852..20322)
                     /locus_tag="IXT53_001679"
     CDS             complement(19852..20322)
                     /locus_tag="IXT53_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HAQ4905503.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(20370..21284)
                     /locus_tag="IXT53_001680"
     CDS             complement(20370..21284)
                     /locus_tag="IXT53_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HAQ4905504.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKNVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            21430..22233
                     /locus_tag="IXT53_001681"
     CDS             21430..22233
                     /locus_tag="IXT53_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HAQ4905505.1"
                     /translation="MTTKQLIISSFIRFKDLKETRNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKELR
                     SFLSKASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            22411..23358
                     /locus_tag="IXT53_001682"
     CDS             22411..23358
                     /locus_tag="IXT53_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAQ4905506.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
BASE COUNT         7097 a         4695 c         3955 g         7658 t
ORIGIN      
        1 aaaaactggc gtagctcttt tacaagccac accagctttt gatacgttgt ttattttaca
       61 tattcatgaa caagttcaac gacttcttcc atcgtatcgc attctttcaa tgcacgatca
      121 gcaagttcag ccattttcgc tgtgtccaag cgtttcatta aactacgcgt tttaagaata
      181 gatgtcgcac tcattgagaa ctcatctaga cccataccta ctagcaatgg aacagctgtt
      241 tgatctccag ccatttctcc acacatacct gcccattttc cttcagcgtg tgctgcatca
      301 attacatttt tgatcaaacg taaaattgat gggttatatg gttggtacaa gtaagaaacg
      361 cgttcgttca tacggtctgc tgccattgtg tattgaatca aatcgttcgt acctacagag
      421 aagaaatcta cttcttttgc aaacttgtct gctaagactg ctgctgctgg aatctcgatc
      481 atgatgccga cttggatcgt atcagatact tccttgcctt cacttatcaa tttttgtttt
      541 tcttcttcaa agatcgcttt tgctgcgcgg aattctttga gtgtagctac catagggaac
      601 atgatacgta aattaccatg aacagatgca cgaagcaatg cacgcatttg tgtacggaac
      661 atttcgtcgc cttgttcaga taaactgata cgcaatgcac gatatcctaa gaatggattc
      721 atttcatgag gtaattgcag atatggtagt tctttatctc caccgatgtc cattgtacga
      781 acaactacag gttttccttc cataccttct aatacagctt tgtaagcttc atattggtca
      841 tcttctgttg ggaagtctgg agaatccata tacaggaatt cagtacggta cagaccaact
      901 gcttctccac cattattatg gacaccaact agatcttttg gtgttccgat gtttgcagct
      961 aattcaaaat gcttgccgtc agctgtgaca gtttctgcat tttttaattt ttcccattct
     1021 gctttttgtg ctgcgtaatc tgcacctgct ttttcaaatt cagctttttg ttcatctgta
     1081 ggatcgatga ttacatcgcc ttcgatcccg ttgacagcca aaatatcgcc ttctttgact
     1141 ttagcagtaa tttcttttgt cccaacgatt gctggaattt ctaaagaacg tgccatgata
     1201 gcagaatgtg aagtacgtcc accaatgtct gttacaaacg cttttacaaa attgcgatct
     1261 aattgtgctg tatcacttgg agtcaagtca tgtgcaacaa cgaccacttc ttcgttgatc
     1321 attgatggat ttggtaaagt aacgcctaat aagtgcgcta aaatacgttt agcaacgtca
     1381 cgaatatctg cagcacgctc ttgcatataa gcattatctt ccattgcttc gaacataccg
     1441 atatacatat ccgttacttc ttttagcgct gattcagcat ttacgctgtt gtctttaata
     1501 ttttgcttga tttgacctac catttctgga tctgaaagaa ccatcaaatg ggcatcaaat
     1561 acttgcgctt ctgcttcacc taagctttgc gctgcttttt cacgaatttg ctgcaattct
     1621 tcagtagatt tagctaatgc agcatccaaa cgagcttctt cagccgcagt atcttctact
     1681 gttgccttac tgaatgttaa atccggttga actagcaggt aagcttttgc aacggctaca
     1741 ccgtcactag cggcaatccc ttttagcatt tcaaccatta ttcagataag ccttctttct
     1801 tcattgtttc aacgattgct gccattgcat cagcttcgtc agcaccttct gcagtaatag
     1861 taacatcaga accttgacct acacctaaag acataacgcc catgattgat ttcaagttta
     1921 ctgatttacc tttatactct aagtttacat cagagttaaa tttgcttgct gtttgtacta
     1981 atagtgtagc tggacgagcg tggatcccag tttctgctac tacgtgaaat tctttctttt
     2041 ccatattaat cggtctcctt taagtgagtg ttgtttttat gtgagggttt tcttttttag
     2101 taattcagag aatcctaaca aatgccaccc tttcatctgt aaagattaac attttttttc
     2161 gttagataca acagttttca ttcaatttca gataaaagta ttcaaattat tccggattct
     2221 ttttcatcct tttcttcaca ttttataaca aacgtgttta tttccttttt cttgtcgctt
     2281 tctcttctcc tgacgtttat cattctttcc tttgaccctc tttgttttca tatgaaacga
     2341 attacttgct tcccttttta tttttagtat aataagagtc aaaggtcagg aatggtcaat
     2401 agagatttca ccttttctac ctcataaatc tcttaaaaaa ctaggggtta ttcttttacc
     2461 tcatgaaaaa cgagatgtaa aatatggatg tacccatttt tgtgaaagga cgtgaatgat
     2521 tcatgctttg tcaaaactgc ggcaagaatg aggcaacgat tcacttgtat gccactgtca
     2581 atggtcagcg gacacaactc gattactgtc agagctgtta tcaaaaaatc aaaaatcaac
     2641 aaaatggagg tctgactaat atggcccaaa atgatccatt tggatttggc agtttggacg
     2701 atcttttccg ttccatgtct cgccaaatgc aacagcaagg aaattatgag caaacaccac
     2761 caacacaatt tggcggaaac aacaacttca atggtggtca gccacctcaa ggcaatacag
     2821 aaggtttatt gggagaatac ggaatcaata tcacagaaca agcacgacaa ggtgatatcg
     2881 atcctgtgat cggacgcgat gacgaaatca aacgagtcat tgaaatttta aatcgccgaa
     2941 ccaagaacaa tcctgtcttg attggagaac caggtgttgg taaaacagca gttgttgaag
     3001 gtttagcaca aaaaatcgta gatggtgatg taccgcaaaa attaatggac aaagaagtca
     3061 ttcgtttaga tgtcgtatct ttagtccaag gtactggtat acgaggacaa tttgaagaac
     3121 gtatgcaaaa attgattgaa gagatccgtc aagcagaaaa tgtgatccta ttcatagatg
     3181 aagttcacga aatcgtcggt gctggttctg ctggtgatgg aaatatggat gctggtaata
     3241 ttttaaaacc agctttagct cgaggagaat tgcaaatggt tggtgctact accttaaatg
     3301 agtaccgcat catcgaaaaa gacgctgctt tagaacgtcg tatgcaacct gtacgggtag
     3361 atgaaccaac tgtcgaagaa acgatcagta ttttgaaagg tttgcaaaaa cgttatgaag
     3421 actatcatca tgtaaaatat actgatgagg ccatcgaagc agccgcaact ttatccaatc
     3481 gttacgtcca agatcgcttc ttgccggata aagccatcga tttgctagat gaaacaggtt
     3541 ctaagaaaaa cttgacgatc caaatcgttg atcctaaaac aatcgagaaa aaactgcaag
     3601 aagcagaaga acaaaaagtt ttagcttctc gagaagaaga tttcgaaaaa gcagcttatt
     3661 atcgcgatca gatcaataaa cttcaaaaaa tgaaagaacg tcagctcact gaagaggaaa
     3721 cacctgtgat taccgaaaaa gatatggaaa aaatcgttga acaacggaca ggtattccag
     3781 tgggtgaatt aaaagaaaaa gaacagacac aactgaaaaa cttagcagat gacttgaaag
     3841 ctcatgtcat cggacaagat aacgctgtag atcgagtagc gaaagcgatt cgccgaaacc
     3901 gtgttggatt aaacaaacag aatcgtccaa tcggctcctt cttatttgtt ggaccaactg
     3961 gtgtaggtaa aacagagctt gcgaaacaac ttgcctacga actattcggt tcacaagatt
     4021 caatgattcg tttcgatatg tctgaatata tggaaaaaca tagtgtctct aaattgattg
     4081 gttctcctcc aggttatgtc ggatacgaag aagctggtca attaacagaa aaagtacgga
     4141 gaaatcctta cagtcttgtc ttgttagatg aagtagaaaa agctcatcca gatgttttgc
     4201 atatgttctt gcaaatctta gatgatggtc gattgacaga tgcacaagga agaacggtca
     4261 gctttaaaga tacgatcatc attatgacaa gtaatgccgg aactggtaaa gtagaagcaa
     4321 acgttggttt tggtgcggca cgcgaaggtg ttactcgttc tgtattgaac caattaaaca
     4381 actactttac accagaattt ctgaaccgtt ttgacgggat catcgaattc agtgcattat
     4441 caaaagaaaa cttgatgaca atcgtcacac taatgctaga cgatgtcaac caaatgctgg
     4501 cagcacagca acttcacatc gaagttccta cgaatgtcaa agaaaaatta gttgacttag
     4561 gctatgatcc atctatgggt gctcgtcctc ttcgacgcac gatccaagaa caaatcgaag
     4621 atggtatcgc tgaattttat cttgatcacc cttctatcca tgagttgaaa gcaaaactgg
     4681 ataaagatgg aaaaatcatc gttacttcta aaccagaacg tttggctaaa gaatcagccg
     4741 aagaaacggc tgaataaatc cagttttata gcgtgagacg gtatttcttt gagaaatgcc
     4801 gtctcattct tttttcagaa aagtcaaaat actgttcatt ttataaaaaa gaaattataa
     4861 taagagaaac aagcaattaa aaatacataa aagaggtgag cgccatgaaa ttggtaaatg
     4921 taacaaacag ccattcccgt cttgtattaa accagcttga aaacactgat gcccatctag
     4981 tcaaggtcta cacagccgga aataccacta ttgtctacac tgaagcccct gaacataatg
     5041 aaattctgct gatcaatgac aaacgtaaaa tccagccgaa agaaatcgaa gatgctaaaa
     5101 attatttctt agataaaatc aaagacgccg tttaccacga ggatgaaatc aaagtaatcg
     5161 aattggcagg ccttgttgaa atctcgattc caaaacatga attaggcgct acattagccg
     5221 tgtaaacaaa ttttgaacat aataattaaa gagcgtggga caaaagtaaa gaacactttt
     5281 gtctcacgct ctaaacacgt ataaacggcg aaagcagaag caactccttc ggaaataagc
     5341 tgaaattcac gaaaattgct tttcaaattt ttgtgaattc cagcttattt cttggagtta
     5401 aacgcttctg tctcagcctc tttttctttc tagctttttg atttttctct tttcttttgt
     5461 acttttctct tttcattttc agttctacgc gtgtctttat ttttaacttt ttcctcttta
     5521 tggatgattg atcgtacttt ttgtgcaatt tgctcgtttt tgtcccagtc ttttacatct
     5581 aataatcgac ataatcgtaa catctcttct tttttctgtt tttttctttc acgtctttgc
     5641 cattctgttt ccatgtgtca cactctcctt tctttaatca tatgtacgca aaataatttt
     5701 tatatttttt tgattagaca tttctatgat tttaatatag taaaaagagc cgtcacggaa
     5761 aatccgtcac ggctctaaac aaaatatgag aggctgtttt tgtttggcta ttcttattac
     5821 ttgatgcagg gcagcttgtg tcaagacttc tttacatcaa actttttaat tttacattgg
     5881 gatatttatc cgcaaaccag cgttctgcaa attggttctc gaataagaac aacggttggt
     5941 cgaaacggtc acgagccaag atattgcgac ttgaactcat cttctcatcc aaatcttctg
     6001 gatcgatcca gcgagcgatt ttgtgtccca ttggagacat gattacttcg gcgttatact
     6061 catttaacat acgatattgg aatacttcga attgtagctg accaacagct ccaatgatat
     6121 attcttccgt tagatatgtt ttataaagct gaatcgctcc ttcttgaact aattgataga
     6181 ttcctttgtg gaaggatttt tgtttcatga cattttttgc agtcactttc ataaacagct
     6241 ctggagtaaa tgaagggagt tcttcataag caacttttag ttttccttcg aacaatgtat
     6301 ccccaatctg ataattgcct gtatcatata caccgatgat atcgcctgct actgcttttt
     6361 cgacgttttc tctcgaatct gccataaatt gtgtgacatt gcttaatttc agttttttat
     6421 ttgtacgttc caaccagaca tccattcctc gttcgaatgt tccagaacag attcggacaa
     6481 aagcgattcg gtcacgatgg gctggattca tatttgcttg gatcttgaat acgaaacctg
     6541 aaaattcttc ctcataagga cttaccatct gatcatcttg tgttttgtgt ccataaggag
     6601 ctggtgccaa ttcaacgaaa gtctctaaaa aagtctgtac accgaagtta gtcaatgcag
     6661 aaccaaaaaa tactggtgtc tgatcgccac gagcaatttt ttcttgatta aatgcatctc
     6721 cagcctcttt gattagttct acttcttcta agacttgttc atataaggaa ttttcagtta
     6781 gaggatgatt ttcgggaagc agtccatctt catccaattc tactaatcgt tcatcctgat
     6841 aattttccgg acgataaaac tctacacgat tatggtagat atcataaaga ccttctagcc
     6901 ctttacccat accgattggc caattcatcg gataagattc gatatctaat agctcttcta
     6961 attcttctaa caattccaat ggttcacgac catcacgatc tagtttgttg ataaaagtaa
     7021 atatcgggat gccacgtttt tttacaactt ggaataattt ctttgtctgt gcttcgatcc
     7081 cttttgcact atcgattacc atgaccgcac tatccacggc catcaaagta cgataagtat
     7141 cttcagaaaa atcttcatgt cctggtgtat ccaagatatt tactcgtttc ccttgataat
     7201 caaactgcat aacagaacta gtaacagaaa tcccacgttg tttctcgatt tccatccagt
     7261 ctgatttggc aaaattacct gtttttttgc cttttacggt cccggcttga cggatagccc
     7321 caccaaacaa tagtaattgt tctgtaatcg ttgttttccc agcatccgga tgggaaatga
     7381 tcgcaaaagt tctgcgattg tccacttgtt gctttaattt tggattattc ataacctaac
     7441 tccatttctt ccattcaatt atttatattt tctctaggca cagattgtcc gatctcttaa
     7501 agaaagacaa tttcatatag ggctgtgaca aaagccgtgt agcagtctct ttttccgagt
     7561 aaacggcgtt ctagagacag cctatcagca ataagcccac ccaagccaaa aatacgaaaa
     7621 gctgtttttg cttggctgct cttattactt aatgcaggat agctttttca caacctttgt
     7681 caactactac aactgaatca gtatagcatg gattcagcga atgaaaaagc caccaaacca
     7741 taagagtcaa atattttttt gtcagctgac taaaagaaaa gacgtgttgc ttgtttattc
     7801 aagcaacacg tctttcataa ccaagtttcc aattatcttc ttggctcgtc atcttcgaat
     7861 tcttttctga agtatcttcg ttcttcttca ggttccgctt ctgtttcttc cggatcatga
     7921 aaaatgacac ggattttaag gattcgagaa ccttccattt cttcagaaac cagcgtaata
     7981 ttatcgacat caaacgataa cttttcgcct tcatccggaa tcatgcctaa tgctgtaatc
     8041 aaataaccag ccatcgtatc tacatcactc atatgcagat cactgtcaaa agcttcattg
     8101 aactcatcaa tcagcatacg tccttggatg atatattcgt gttcatcgat tttttcataa
     8161 agattctcaa cttcatctga ttcatcatcg atttctccga cgatttcttc taaaaggtct
     8221 tctagtgttg ctaacccaac gacgccgcca tattcatcta ataaaatagc catctgattc
     8281 tgcgtctttt tcatttcata taacagatca tcaataaaga ccgtttcagg aacgaataaa
     8341 ggctcctgca tgattttctt gatatctaaa ttatcaaagc cgaatttatg ggcagctttt
     8401 agtaaattct ttgtatgaag gattccgact actttatctt tgtcttcgtt ataaacaggg
     8461 attcttgaat agttttcgga taaaacttca ttcacatttt cttccacagt gtcgttgata
     8521 tcaatcataa atgcatccgt tcgtgggacc atgacttctc gggcaacttt cgtatcaaga
     8581 gaaaaaaccc cttggagcat ttctaattct tcattattca atacgccttc gttttcaagc
     8641 atataacgca tttcatctct ggtcattttt gaatcttcat catcaaaagt catcggcgtg
     8701 atacgcgcca ataaacttgt ggaagcagaa agcagccaga cgaagggctt agctactaca
     8761 ccaatcaagc gtatcagccc tgaagtgaac tttgcgactt cttctgattt atttaaagca
     8821 atacgtttag ggtacaattc tccaaaaaca atcgatacat aggtcaaaat agccagcaca
     8881 atgatatttg caatattttt tgccgctgtg ccgccaccta ataccggagc taaccgttcc
     8941 gataaagtat tagctagtga agcaccagac aaaatgttga ccaaagtgat tcctacttgg
     9001 attgtcgata ggaaattatt tggattctgc aatattttta aaagcttttg agatttcaca
     9061 tctccttcat cagctttttg ctcgattcga ttcttattga tcgaaacaac agcaatttcg
     9121 gaagctgcta aaaaagcatt gatcaatgtc aaaacgatca ataataaaat ctgcgctaac
     9181 agcgactgac tctcagggtc agcattcata atcgcattct ctccttataa aattaatttt
     9241 tagtttacga tagaaaaaaa cgtggcacta tagccacgtt aaaatggtat cacaaagcaa
     9301 ttttctttgc aataaaaaca agtgtactaa aactacctac gaggtcgtag caattttctt
     9361 cttgttttca ttttgcttgt cttctttgac gattttgatt atataggaaa tgatcgtctt
     9421 gcagattgcg taaaaaggaa cacctaagaa gataccaagc aatccggcaa tatttccagc
     9481 tactagaaga acgataatga tcgttaatgg atggattttc agtgatttcc caattacatt
     9541 aggataaatg atattcccat ccaactgttg gacgattagc acaaccacac aacaaagtaa
     9601 tgcttttacc ggatcattga agactgttac tagaaatgca ggtgctagtc ctagataagg
     9661 tcctaaatat ggaattaggt ttgtcaatcc cgcaatgacc ccaaataaga aagcataatc
     9721 cacaccaatt accatatatc ctaaaaatgt gaaggtacca acgaataaac attcgattgc
     9781 ttgtccgcta atgtaattag ccaatgtttt attcagttgc cccaataact ccacaatttg
     9841 gtttcttctt ttttctggga aaaactgttg gatgtttggc actagttttt ctccgtcttt
     9901 caacatatag aacaagatga atggtgctgt aatgattact acggttgccg aagcgatcgt
     9961 tccaacgatc gaacctaaac tattggatac tccactaagg aattgctgaa tgattgtccc
    10021 gtaagaaatg tctaattgct caaaatattt attgatatct acttccttga acaaaggcaa
    10081 ttcggctacc tcttttaacc agctttctac ctgtttgatg aaatcaggca tattagatgc
    10141 caatgaggaa atttgctgta ctagacttgg gatgacacta agtaaaatcc aaataattgc
    10201 tgcaactaaa aggagcagca caattgcgac agcccatatt cttttgattt ttgctttcat
    10261 caacagcttg acaataggat tcaataaata gtaaagaaag cctgcaatca gtacaggtgc
    10321 aaataacgtg gaaaaaaatg tcccgatcgg ttcgaaaata aagtttattt ctgatgaaat
    10381 catgatcaat gcagctatga tcaataattc aagcgaccaa aacattagtt tagactgtct
    10441 aagtttttca aacaatagat ttcctccttt ctttcttact agtatatatt atattcgtta
    10501 aaaattcttt tattcttttt ttatttttga aaacttgcca tactttttat ttgttttatt
    10561 ttccagtata ctatacgaaa ggaaggtggc tagaccatga aagttgtaca aacaaaagat
    10621 acaatgagca atatttactt agatgctgta cggatcaggc atcaagtttt tgtagtcgaa
    10681 cagggtgtgc ctttgtctcg agaaatagac aaagatgaag cccattgtat ccacttcgtc
    10741 ctgtactcag ataaaaaaga accgcaagga actgtacgtc ttttgccttt agaaaacgga
    10801 aaaatgaaat tacagcggat ggcgattctt tctgaataca gacaccaagg gttaggaaaa
    10861 atattgattg aagaagcaga aaactttgct aagaatcaag gctataatac gattttatta
    10921 ggggcacagt ccacagctga aactttttac gaaaaactag gatatacagc ttacggcgat
    10981 ccttttgaag atgctggcat gcctcatatt tatatgaaaa agactttata aaaatttatt
    11041 tcgaaaaaag caaaaaacag caagcttttg agaaaaatct ctcactggct tactgttctt
    11101 ttttgtgttt acttacgcga caattgcttg taacggttat accaaatatc aatatagcct
    11161 tccgagaacg ggccatctcc attattgatc cagtcaacca gaatcttcac attttctttt
    11221 aggatgaagt caatatcgtt cgggtaattc atcattttgc tgtgcatctc atattcatcg
    11281 acatccaata gtcgtttctc gccgtcagga aataccttga tatccaagtc atagtcgata
    11341 tatttcaagg cctcatcgtc cagcaaaaaa ggtgaagcta gattacaata ataggaaacc
    11401 cccttttctc tgatcattgc tattacattg aaccagtatt ttttatgaaa ataaacaatc
    11461 gctggttcgc gcgttaccca tcggcgtcca tcagattcag tgactaatgt gtgatcattc
    11521 acgccgatca tagagtgttc gcttgttttt aataccatgg tatctcgcca cgttcgatgc
    11581 aatttgccgt cgtgcttgta actttggatc gtcacaaact ctccttcttt tggaactccc
    11641 atagcaaccc ttccttaagc agcattttgt gctttgtgtt tgggtgttca ttacaccgac
    11701 atcataatta ttatatcaat aaaaatgagc tttgcctaga aaggattcct attgtttcga
    11761 tttttctcta actgatctac cagtttcatt tggaccttag gaaaaacgac cgaatcaaat
    11821 gcatcaggct tcaaccactt cgaagttttg ttctcggtaa attcttgttc tgctccctcg
    11881 gttgcccgtc cgtaaaaaag gagaacatgc catttcagat gactaaaaat atgagtaact
    11941 tctcctaaat gtcgtgtctg ccaaacaaag agttcctgct tttcaaatgg gagattttgc
    12001 tcttcttctg ctactaggtc atcaaacaga tcgatttctg gctttgtttc ccattctttt
    12061 ttcaatcgct catactcctc ttgtgtcact tccatcattg gaaatgtcca catattcgcc
    12121 agcaattttt ggctatctct ttcttcaaaa taatacgctc cagaatgatt ttgaagagct
    12181 gcactgatat aataaacgtc ttttggcttt gctttttttg tttttacagg aaaactcgtt
    12241 tggattcctc gtttgttagc taaacaaaag gcttggatcg gacaagtctc acactttgga
    12301 gaagttggtg tacagatagc tgaacctaga tccatcattg cttggttgaa ttcacctgga
    12361 tgcttttcat caatgatttt ccgcatcgct tcatcaaaga ttttcctgct agaagctttt
    12421 gcgatgtctg cttcaatgca aaacaatctg ctgactactc gcatcacgtt gccgtcaact
    12481 gcaggttctg gaagtccaaa tgcgatactt gcaatcgcgc ctgtcgtata tggtcctatt
    12541 cccttcaatg agctaatttc ttcaggcgtt tgcggcattt ccccatcaaa ttctgacatg
    12601 atctgtttag cagcagcttg gatgttccgg gctcttgagt aataaccaag gccctcccat
    12661 gcttttaaaa gcttttcttc tggagcattt gctagctctt caattgtagg aaaccattcc
    12721 atgaaacgat aaaaataatc gatcactgtg tctactctgg tttgctgaag catgatctca
    12781 gagatccaga tacgataagg atcacgatta tagcgccaag gcaggtttct tttttcttgt
    12841 tcataccatt gaatgaactg atcttgaaat tcttttgttt ctttatctgt ccatttttcc
    12901 cattctttat tcactttcct cttccttttc cgtaatgaat cgctggatca tatccaaatc
    12961 aaatcctttt tgatataagc tttgctttac ttttagattt ctttttttag gttcaaacct
    13021 taaatttctt cgccatagtt tatctccttg tttgactaaa taatcatatt ctatttcttg
    13081 atctgcttct tgcggaagat cagctatcgc ttgttggatc acttcttgag aaaagccttt
    13141 cgtcatcaat gtttgttgga tttttctcaa taattctttt tggctttttc cgtgattttt
    13201 tccaaacgct ttttcggctg tctgatgtgc attttgaact tggtcttcaa atgtatattg
    13261 gctgagcgct tgttcaatga tttcaggttt caaacctttt tgctgcatct gctgcgctaa
    13321 tttctttggt cctttgtcac ttaagcgcat attcgtgcgc acataacttt ccccatagac
    13381 caaatcatcg acaaggtcca attcttttaa gcgggctacg attttatttc gatcttcttg
    13441 aaggatttcc ttttctttta agtatgttcg gatttctttt tccgatcgca actgataact
    13501 taagtaattc attgctaggt gaaaaccgaa atcagctccg ctactcttct tgatctcttg
    13561 gatagtttct tcgtctaatt cccgtccttt taataatcga tagcgtacta atacatcttc
    13621 agatacttgt aaagcttctc cttcagataa atctacgcga tagaaagggc ctttgccttt
    13681 actgattctc gtaacggtta acataaacat cctcctcgaa catatattct ctatcatacc
    13741 acagaaaatc cccgtaatca ggtattatat gttagaataa accacagaaa aaagaaaaag
    13801 ggagatactt atggcagaaa caattgtcaa aactggacaa acggttcctt tgaaaatcaa
    13861 gcgtttaggc ataaatggtg aaggaatcgg ttatttcaaa cggttgatca tatttgttcc
    13921 atatgcacta ccaaaagaag aagtactagt gaaaatcacg aaagcaactc ctcgctatgc
    13981 agaaggagaa ttaataaaag tcaaaaaaac aagcaaagat cgcgtagtgg caccatgtcc
    14041 tgtttactat gaatgcggtg gctgtcaatt acaacactta gcttatcaag ctcaattgga
    14101 ttttaaaaag gatctgcttt tgcaagcatt ggaaaaattc aaaccagcag gctttcgatc
    14161 ttatcaactt ttgccgacga tcgggatgga tgagccttgg cattatagaa acaaagcaca
    14221 atttcagtta cgaaaaaata agcagactca aaaaattgaa gctggtttat acgaagcaaa
    14281 ctctcatcag ttagtcccta taacagactg cttggttcaa gaacctcgaa cacaaaaagt
    14341 aatgaatact gttgttcaac tattgaataa atttgatgca cccatctatg atgaacggac
    14401 aaacagtgga atcttccgca caatcatggt tagaatcggt gttaaaactg gggagttaca
    14461 agttgttttt attactcgca gtaaaaaatt cccgcaaaaa aacgcaatga tccgtgagat
    14521 caatgaacga ctgcccgaag ttgtttcaat catgcaaaat gtccaggcac aaaaaacgtc
    14581 tctcgtgatg ggggatgaaa cgatccactt atggggaaaa gaagccattg aagaacaaat
    14641 caatgaagtg actttcgatc tttctcctcg tgccttcttt caactcaatc ctcagcagac
    14701 cgaattgctt tatggcgaag gaatcaaagc attagatgtc cagcctaatg aaacgatcgt
    14761 ggatgcttat tgcggagttg ggacgatcgg cttaagtgta gcaaaacaag ccaaagaagt
    14821 acggggaatg gataccgttc ctcaagcaat cgctgatgca aaaaaaaatg cggaaagatt
    14881 aggatatcac aacactcgat atgaagtcgg gacagcagag caagttctgc ctaaatggtt
    14941 gaatgaaggt tttcagcctg atggtatcat cgtagatcca ccacgtacgg gtttggataa
    15001 cgcattgatc caatcattat tgaaataccc tccaaaaaaa cttgtttata tctcctgtaa
    15061 tgtttctaca cttgccagag atctggttaa tttaagtaaa aagttcaaga ttgaatattt
    15121 acagtccgtc gacatgtttc ctcagactgc acgttgtgaa gttgtcgtga agatgacgag
    15181 actcgctgac taaatgaaag aggttgtgaa aaagtctgcc ctgtatcaag taataagaac
    15241 agccaaacaa aaatagctct tcatgtttta tgaatactgt acttgtgacc gaagagttaa
    15301 caggaggaca ctatttattt aaaaaaaggg ttgtgacata gtttttgtca cagccctttt
    15361 tgattattat tggatattca cgtctatcac ttgataaaat gcatttgtcg tatcgctgat
    15421 attccatact gctaaaatca cattatatcc ttttcgatcc tgcggaagtg tgatcgtttg
    15481 gttgactgtt tttggtggaa cagttgcttt gtcgtctatt tttgtgatca attcaaaatc
    15541 actaaatttc aaaggttgat taggattcca agttggtttg gtaatgtagt aatcccaagt
    15601 acttgtccga tgttgtgctg ttaaattcca agtaatattt aatgttcctg gattaaccat
    15661 gtttttatgc catctgttag atgtctgttc atctaaaggc tcaaagccgc tgactcctgc
    15721 actagcaatt tttccatcaa tgaatgtatt tttggtagct tcgatacttt gcggttccca
    15781 ttgtgctctt ccgacatttt gatttaagtt tcctgctgct gatgttccta ggtatgcacg
    15841 gcttcctgga gaagtaatat aaccatgcgc aaaacctgga atgctaaaac cgaacaaccc
    15901 taaactaact aacccaataa acaataacgt actaatcttt ttcatgtaga aacctccttc
    15961 atttgataac cacattgtaa acgctttatt gtattaacac aaacattatg cttttataat
    16021 tttatgttga agtaaaaaag gtgaaaaaag taagatgcag attataacag aagtagacga
    16081 gaaaagtaca aatagaaaac atgagaatca aatcatatga tctttttcaa aaacatcatt
    16141 catataaaca aatgttcaac tttttaataa caacaattat caattttttt cttacttcac
    16201 aaactaaata caggtcttgc tgtgagcctt catggtaaac taatattata gaaaggagtt
    16261 agtaaaatga agatgataaa agatattcgt ttttgggttt gtgttatagg aattgtcata
    16321 ttaggctttt tatcagggtt gcttagcgga aatcccggaa aatattacta ttctttgcaa
    16381 cttccaccat ttgcaccacc tagctggata tttgggccga tgtggacctt actgtatata
    16441 ttgatgggta tttctcttta tttgttactt aatcacaaca acaaaaaaca gcgaaacaat
    16501 cttgtcggtc tttttgtgat tcagttcatt tttaatttta tatggtctgc tctatttttt
    16561 aatttaagaa atattttcat tgcagcaatc gatattacac ttttagttat tttcttgtca
    16621 gtgcttatgt atcagttgtg gctgcatcat cgcctggcaa tgtggctcat gattccttat
    16681 tatttatggg tactattcgc aactctattg aattattcaa tttatttttt aaactaagcg
    16741 ctaaaaagag acgtcctgta atttaacaca tagaaaaagt gccacctaag ttagattttt
    16801 tgtctaattt aggtggcact ctttattaac caaactattt ttttctagta cccacttgtt
    16861 gttccatact cattattctg gtcataacca taaccatttt gttcctgtcc agcgtaagga
    16921 tcataacttt gagtatcttg acttggtgta tagtactgac ttgtatcttc tgtttgctga
    16981 taataggtat tcggatcttg gtactgatca tatgtgtttt ctgaatagga agaataatcg
    17041 gtgtaccctt cactattcga tccatcaccg tttgtcgaat cgccatcttc cgaatcatca
    17101 aagaacatca ttgaagcatt ctcatttttc aaattaggta atgtatcgct tgtttttata
    17161 tttaattgtt tcttcagttc attttggatc gaaagaagat cattttttcc taaaatttga
    17221 tagtagatat cattgatcat ttgtccttcg ccttgcaact ggtcttgttt gattgtttca
    17281 aacgccgggg tgtagttcgt cgctacatca agcatgtcat cccaatctaa gtccgtcgtc
    17341 acattgttct caactgcttt taaaattttt ctataattgc ttacgccatc taaacttaaa
    17401 actttattga cgattttcgt cactacttcc cgctgacgac gttgtcgacc gatatcgcct
    17461 tctggatctt cgtgtcgcat tcgagaatag gctaaacctt tttctccatt caatgtttgt
    17521 tcacctactg gtacatggac accatccaaa gtaaagtcaa ttttattatt cactttgatg
    17581 ccaccgaccg cattgatcaa atcttccatg ccatccaaat tgatcgttac ataatgatca
    17641 ataggaatat cgagcagttg ctcaattgaa tccatggcca tttcgacacc accaaatgcg
    17701 taggcatgat ttaatttatc taccgttcca taacctacga tattcgtata aatatctcgg
    17761 tccaaactga tgatcgttga ttttttttgt ttcggattaa ctgttactac catcatcgta
    17821 tcagaacgtc cttgttccgt tcttcctaat ccaccggtat ctaaacctaa tagcaaaacc
    17881 gaaaaaggtt ctttgtcatc aatgcttact ctctcagaac gtttggagga tattcgatta
    17941 gagtttttag aaattttgtt tactgtttgg ctcgcttctc ccataaatct gattccgtat
    18001 gctgataatc cggcaatcag caaaacgaat acccctaaca aggttaatag aactttttgc
    18061 catcgcttca taatacacct ctgtttcaca ttttctaaaa tattgtctca tataactgtt
    18121 caatcagcaa attcactttt taatcataag aaaactttaa aaaacatttt tgtgtaacga
    18181 ttattatttt ttaaaatttc tcaaaattct taactatcat accataccgt gaaaaagatc
    18241 gaaagaccga atacacgcag ttcgcaatcg ttttatcttt ttttatgtat tttttccaga
    18301 aaaagcgaac aacaaggtat tttccaaata atcgtgcagt aaaatgatcg cacatacctt
    18361 tcccatcttt ttaacagtct ttgcctaccg ttcagattat cattcgattt cacgacaatt
    18421 tcttcctatg actggtcctt ttgttaaacc catcttctat tcgttttatt tccagctcac
    18481 tgcatggctc ttaaaatgac tcgaactagt gattgcatta ccatcatctt ttaatggata
    18541 gatatgctcc tcttcaaagt aaaacgattt tcttttaaag taatagaatt gacatggtaa
    18601 tgagtatgta tactagtggt ttcacaaatg gtcttttatt caacatccaa aaactgaaat
    18661 ttctttaaaa tccattacta acgaagaaaa tgttccttgc ttgataatta gttaggacat
    18721 gtcaagtgaa caaatgcatt tacttagcca aaaaatctaa aaacttcata aatagacaaa
    18781 aagacacgac tctctaagaa gtcgtgtctt tattcaacaa attattataa tttagtcact
    18841 tcagcagctt gaggtccgcg tgcaccttca acaatcgtga attctacttc ttgaccttct
    18901 tctaggctct tgaagccgtc tccttgaatt gctgagaaat gaacaaatac gtcatctccg
    18961 ccgtctactg taataaaccc aaatcctttt tcattgttga accatttaac cgttccgtgt
    19021 tccataaatg tatagcctcc aaaaaagttc ttgcaagtaa ccttgcaaga acattatacc
    19081 caatttagaa aacgctagca agctttcgct cttttatttt ccaaattatt tttaagactg
    19141 atatgacgga ggaaatggtt attttgctgc ttcgaaacgt ttgtttactt catcccaatt
    19201 aataacattc cagaatgctg caatataatc tggtcggaca tttttatatt tcaaataata
    19261 agcatgttcc caaacgtcca aacctagaat tggtgtcttt ccttccatca atggagaatc
    19321 ttgatttgca gtagaggtaa tagctaattt tccattttcc attacaagcc aagcccatcc
    19381 agaaccaaat cgtccagcgg ctgctttttt gaattcttct ttaaaagaag aaaaatcacc
    19441 aaaagcttca ttaatcgctt cttttatttc tcctgtaggt tcgccaccag catttggtgc
    19501 catgatttcc cagaaaaatg aatggttagc atgtccgcca ccattgttac gtacagctgt
    19561 cttgatatct gttggaatag cgtccatatc agataataat tcttctattg ttttttcgcc
    19621 taattctggg tatttctcaa ttgctgcatt taaattcgtc acataagtat tgtgatgctt
    19681 atcatgatgc agatgcatcg tttcttcgtc aatataaggt tccaatgcgt catatgcata
    19741 aggtagatct ggtaatgtgt aagtcataaa aaaattcctc ctcaatgttt tcttaataat
    19801 gtctacatta caaagagtaa caaagagaag gaaattcttc aattgttatg cctagtcgtc
    19861 tttttcactt tgacctactt cttcttcaaa aacctcagtt tctttaaaaa taaacaactt
    19921 gctgaaaata tagttcgcaa ggataacaat aatttgagtg atgattttcg ccacatactc
    19981 attgctgttc ataacattga tcaacaatac cattgccccc atgtccaatc cgtaagaaag
    20041 gatacggtag aagaagaact taccgcattc aataagtgca tgtgtaaaat tcggtgattt
    20101 tgaatggaat acccaaagtt tgttcgttac aaaagcaaac aatacagaac aaaaccacga
    20161 aatagaattg gataaggcca tacttaaatc aaaatattgg cgtgcgagag caaaactaac
    20221 aaaatttaca atcgtagcca aaccaccaaa aaagagatag actagtatct cccaatatcc
    20281 ttttgcttct aaatatcctt ttaactgtcg atactgcttc atgagccaac ctaccttatt
    20341 aaaaattctt atttatcata ctctatattt caataacttt catctttatt taaaaaaatt
    20401 aatttttttt gatcctttgc ctttgtctct gcttggatcg tcacatgtgt aataccatat
    20461 tttaatttta ataaagaaat tatacgagga tatatctttt caatctcgct tagtagctga
    20521 tcttcaaaat caatatgtcc gtcgaaaata attatctttt cgctatactg ccaaatatgg
    20581 acatgatgaa cattctttac cccatcgatt gcttcaattt cttgttccat tttttcatag
    20641 tcaatagcag gtgcactttg tatcaaaata gcggcagctt ttttgatcac cattatcgct
    20701 tctatcaata tatatatcga aatgatcagt gtaataactg gatcgatcca gtagatatcg
    20761 aaaaaacgga tcaaaattgc tccgataatc acacttaccg aagacagtgt atcacttaac
    20821 aaatgcaaat aactagattt tatattcaaa ctttcatctg cttcttgtcg taataataat
    20881 actgagaaca aattcgcgag caatccaata actgctactg ttagcatcaa atgactattt
    20941 atcttttctg gtgaagtgaa ccgcttgaat gcttcaacgg ttaaaaacaa ggataatatg
    21001 atcaatatga atgagttgat gaaagcactc aatatttcta aacgctggta gccatacgta
    21061 cgttttttat ttgcttcttt tcgggccatt ttttgtgccc cgtaagaaaa aataattgcc
    21121 aatgtatcac taaggttatg gaaagcatct gaaacaagag acaaactccc tgacataatc
    21181 cctccaataa attctgataa agtgatcacc gcattcaaaa ccactactcc aaatagtttt
    21241 tttgatgatc tctgtggctg tgtaggcact attccttcct ccattttatt tatattggat
    21301 agtctttaga caagaataac atttttttga tatttcacca atcattcaga tgtttcccta
    21361 aagcacttgt atttcacttg atacttcgct agaattgcaa gtgatgacac tactagatga
    21421 ggagtactta tgacaacaaa acaattaatt atcagttcct ttatacgatt caaagaccta
    21481 aaggagacga gaaatgtccc attttggaaa gccattgtct atcttttagt tttgagtata
    21541 attatggctt tgccaatctc ttttcaagtt tttcaagtct taaacaacat tcaattggat
    21601 gggcaaaaga ttgctactcg cattcctgat tttacaatcc aaaacgggca gcttcaaacc
    21661 gaagaaaaag aaggattcat ttaccaaaca aactccatta tcttcacatt tgatccagag
    21721 ggaaaacgaa cagaacagga catctctact gatttgatgg ggaattttgt tagtgtcggg
    21781 atgttaaaaa acaaacttat catagctctt cctaatacag gcacgacttc tgctttgttg
    21841 aacaataacc aattagaact tccctacacc aatgaatctt taaaaaactt aacaggtaag
    21901 gagctccgtt cttttttaag taaagcatct ataccacttt ggataaaagt cctgacattt
    21961 atattttcta tttatcctag ttttctgaat ctgctgatca ctttgctatt taccaacatt
    22021 gctgcttttc tctatgcacg ctttcgacta acaaaagcta cttttcttga ttgtttaaaa
    22081 acattgatct attcaatttc attgcctact ataattgcaa caatcttgat ggtctttctt
    22141 ccttcttttg acacaagcgc cttcattgct tttgcaggaa tctttatttt tgctcaagct
    22201 gtcaaaggat gggcaaaaat atcgatcaat taagaaataa agacaactcg ttatttctac
    22261 ttcaatagac ccttttagag gttgtgacat ttttttgtca cagccccttt tttatttgaa
    22321 aaatatttct ttctcaaaaa acaagcaagg cgcttcaaag ttcttcttgt atgcgctaca
    22381 cttagagtaa agaaaggcgg gaaattaact atgtcagaaa gaatcattta tgcagatcgt
    22441 ccatttcgtg atgaatttat cgaagaaatc catactatag cgcctggtta tatctttaaa
    22501 acagaattac aaccagagga tctacaaaat gtagagatca gtttgggctg gaactcagac
    22561 taccaagaaa aactattagc ctcttctacc ttgaaatggg tccaatctat ctcagctggt
    22621 gttgatcagc taccccttga gactttttca aagaaacata ttttactttc aaatggtagt
    22681 ggcatccaca gtgaatcgat tacggagcat gtcttaggaa tcatccttgg gtattctcgt
    22741 ggtctttttc aagcacaaaa agcacagagt gacatgaaat ggttaggttc atcggtccat
    22801 taccaatcta tccaaggaaa aaaaatacta atcattggaa ctggccatat tggaaaaatg
    22861 attgcaaaaa aatgcaaagc attagacatg aactgctatg gaatcaatac atccggtcat
    22921 ccagcagaag gaatggaaaa gactttttct ttaaatcaac taaaggaagt tcttcctgaa
    22981 gtagatttcg tagtcaatat cttacctcta acggaggaaa caactgggct ctttgatcag
    23041 catatgtttg aacactttga tccaaaatct gtattcatca atgttggccg tggtgcttct
    23101 gttaagacac aagatctggt gcaggcttta aataaccagc aactttcctt tgcagcatta
    23161 gacgtttttg aggaagagcc tcttccacaa gatcatcctc tttggaaaat ggacaatgta
    23221 ctgattactt cccacattgc aggtttgaca cctgattttc aaaagaaatt aatggctatt
    23281 tttttaacca atctgaaaag ctactttgcc acccatgagc ttcaaaccaa tcaagtaaaa
    23341 ttgtctgcag gatattaata gctgaaaaca atattttcaa aagaacaaag agaaggtgac
    23401 aaaac
//
LOCUS       DACGLW010000044        22941 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.044, whole genome shotgun sequence.
ACCESSION   DACGLW010000044 DACGLW010000000
VERSION     DACGLW010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22941
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.044"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            160..390
                     /locus_tag="IXT53_001683"
     CDS             160..390
                     /locus_tag="IXT53_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HAQ4905507.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            487..1788
                     /gene="murA"
                     /locus_tag="IXT53_001684"
     CDS             487..1788
                     /gene="murA"
                     /locus_tag="IXT53_001684"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAQ4905508.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1801..1977
                     /locus_tag="IXT53_001685"
     CDS             1801..1977
                     /locus_tag="IXT53_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HAQ4905509.1"
                     /translation="MSNQRYIILTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            2112..2408
                     /gene="yidD"
                     /locus_tag="IXT53_001686"
     CDS             2112..2408
                     /gene="yidD"
                     /locus_tag="IXT53_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HAQ4905510.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            2592..3059
                     /locus_tag="IXT53_001687"
     CDS             2592..3059
                     /locus_tag="IXT53_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ4905511.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            3144..4028
                     /locus_tag="IXT53_001688"
     CDS             3144..4028
                     /locus_tag="IXT53_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ4905512.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            4145..4378
                     /locus_tag="IXT53_001689"
     CDS             4145..4378
                     /locus_tag="IXT53_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HAQ4905513.1"
                     /translation="MSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYGP
                     KEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            4466..4546
                     /locus_tag="IXT53_001690"
     tRNA            4466..4546
                     /locus_tag="IXT53_001690"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4500..4502,aa:Tyr,seq:gta)
     gene            4553..4624
                     /locus_tag="IXT53_001691"
     tRNA            4553..4624
                     /locus_tag="IXT53_001691"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4585..4587,aa:Gln,seq:ttg)
     gene            5139..5846
                     /locus_tag="IXT53_001692"
     CDS             5139..5846
                     /locus_tag="IXT53_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ4905514.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(5906..6550)
                     /locus_tag="IXT53_001693"
     CDS             complement(5906..6550)
                     /locus_tag="IXT53_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HAQ4905515.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            6677..6964
                     /locus_tag="IXT53_001694"
     CDS             6677..6964
                     /locus_tag="IXT53_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905516.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            7072..7392
                     /locus_tag="IXT53_001695"
     CDS             7072..7392
                     /locus_tag="IXT53_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ4905517.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            7473..8786
                     /locus_tag="IXT53_001696"
     CDS             7473..8786
                     /locus_tag="IXT53_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HAQ4905518.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            8783..9502
                     /locus_tag="IXT53_001697"
     CDS             8783..9502
                     /locus_tag="IXT53_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HAQ4905519.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            9630..10181
                     /gene="raiA"
                     /locus_tag="IXT53_001698"
     CDS             9630..10181
                     /gene="raiA"
                     /locus_tag="IXT53_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HAQ4905520.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            10403..12937
                     /gene="secA"
                     /locus_tag="IXT53_001699"
     CDS             10403..12937
                     /gene="secA"
                     /locus_tag="IXT53_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HAQ4905521.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDIQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGDE
                     DAVIQSKMLTKQVEAAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIVDFAGSTLVHEDSISLTDIEGKTSQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKDAN"
     gene            13064..14165
                     /gene="prfB"
                     /locus_tag="IXT53_001700"
     CDS             join(13064..13126,13128..14165)
                     /gene="prfB"
                     /locus_tag="IXT53_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078989099.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HAQ4905522.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRGLFDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAVMQEEAEARI
                     NALDEKMNTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            14270..14956
                     /gene="ftsE"
                     /locus_tag="IXT53_001701"
     CDS             14270..14956
                     /gene="ftsE"
                     /locus_tag="IXT53_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HAQ4905523.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            14940..15833
                     /locus_tag="IXT53_001702"
     CDS             14940..15833
                     /locus_tag="IXT53_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ4905524.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEKAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFYQLINPSLLRSHYSLIHPENIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            15985..17433
                     /gene="cls"
                     /locus_tag="IXT53_001703"
     CDS             15985..17433
                     /gene="cls"
                     /locus_tag="IXT53_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAQ4905525.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDTLGE
                     EVREALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPEFFPTIHSTGDMAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLIYDERMASRVRKAFFEDS
                     KISTHLTKEVYENRGMMIKMKEGLARLISPLL"
     gene            17770..18627
                     /locus_tag="IXT53_001704"
     CDS             17770..18627
                     /locus_tag="IXT53_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HAQ4905526.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQAENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            18742..19662
                     /gene="pstC"
                     /locus_tag="IXT53_001705"
     CDS             18742..19662
                     /gene="pstC"
                     /locus_tag="IXT53_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HAQ4905527.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            19662..20546
                     /gene="pstA"
                     /locus_tag="IXT53_001706"
     CDS             19662..20546
                     /gene="pstA"
                     /locus_tag="IXT53_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HAQ4905528.1"
                     /translation="MNAKRSDKIATGVLYVVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            20561..21367
                     /gene="pstB"
                     /locus_tag="IXT53_001707"
     CDS             20561..21367
                     /gene="pstB"
                     /locus_tag="IXT53_001707"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAQ4905529.1"
                     /translation="MKKYNWNETNVITMPKDKEVALYTNDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYKDVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            21383..22141
                     /locus_tag="IXT53_001708"
     CDS             21383..22141
                     /locus_tag="IXT53_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HAQ4905530.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWEDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            22154..22831
                     /gene="phoU"
                     /locus_tag="IXT53_001709"
     CDS             22154..22831
                     /gene="phoU"
                     /locus_tag="IXT53_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HAQ4905531.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
BASE COUNT         7571 a         3923 c         5025 g         6422 t
ORIGIN      
        1 ttgtctcagt cccttttttt gcatattttt agcatttatt tcctaaaaaa tgtaagggga
       61 ttcctatatt tttaagtata cttgtttttc cttttataca ttttatggta ttatcatttt
      121 attagttatt ataaaacaat cgttagaaag gacgtcatta tgcaagtata cggaattgat
      181 gcggtgcttc gaatcgtgag tcatttggcg tttatctact tagccttctg gtctttacgt
      241 tcgttacgca ttgaaacttt tttcagacca atgcataata cgcaaattag aatggcaatc
      301 gtactttttt caattttttt agggtacact gcaagtaatt ttttcttaga agttatcgct
      361 ttatgccgaa atcttttcgt tagcctgtaa attttaaaaa aaggtactat tttaaaagca
      421 aggatgttat aatagaaaag attttgagta agtgtatgct cattggaatt ttttggaggg
      481 aacaacatgg aagagatcat cgtaagaggt ggcaatcaat taaatggaac agtaagaatt
      541 gaaggagcta aaaatgctgt tttgcctatt ttagctgcta gtttgttagc agaagaaggt
      601 attacgacat tagacaacgt tccgattctt tctgatgtct tcactatgaa tcaagtgatt
      661 cgtcacttaa acgtagatgt agattttgat gaacaaaaga atcaagtaac aatcgatgct
      721 tcaagacaat tagaaatcga agcgccttat gagtatgtta gtcaaatgcg tgcttcaatc
      781 gttgtgatgg gtccgctact tgccagaaat ggacatgcaa aagtagctat gcctggtgga
      841 tgtgcgattg gtaaacgacc aatcgatttg catttaaaag gatttcaagc tttaggagca
      901 aaaattattc aaaaaaacgg ctatatcgaa gcaattgcgg atgaattgat cggtaacacg
      961 atttatttag atttccctag tgtaggtgct actcaaaata tcatgatggc tgccgtaaaa
     1021 gcaaaaggaa caaccatcat tgaaaacgta gctcgcgaac cagaaatcgt tgatttagca
     1081 aacattttga ataaaatggg cgctcaagtc tatggagctg gtactgaaac aatgcgtatt
     1141 gaaggcgtgg atcatttaca tgcagtcaac cactcaatcg tacaagatcg tatcgaagca
     1201 ggtaccttta tggttgctgc tgcaatgaca caaggcaatg tgctgattgc agatgctatc
     1261 tctgaacata accgcccatt gatttctaaa ttgatcgaaa tgggtgcaga aatcattgaa
     1321 gaagaaggcg gagtccgtgt catcggacca aaacatatct tacctacaga tgtaaaaacg
     1381 atgcctcatc ctggtttccc tacagatatg caagctcaaa tgacagctat ccaattagtt
     1441 gcagaaggta caagtgtagt gactgaaaca gtgtttgaaa atcgtttcca acatttagaa
     1501 gagatgcgcc gtatgaacgc tcatgtcaaa atcgacggaa atgtagcaat catggatgga
     1561 aaccatgaat tgcaaggtgc agaagtatat gcaacagact tgcgggcagc agcagcatta
     1621 gttttagctg gcttgaaagc aaatggaatc acacgtgtgc gtaacttgaa ttatctagac
     1681 cgtggatatt ataatttcca tatcaaacta cagcaattag gtgcagacgt tgaacgtgta
     1741 gacatggatc aaacttctgc tgaaaaaaca gcacaaacga ttgcttaagg aagtggctga
     1801 atgagtaacc aacgatatat catattaact ttgataaaga ttttagtggt gatcttattg
     1861 cttatccttc tttttgtggc cggaacgatg atcggatatg gtgtcatcgg tggcggcaat
     1921 ccatttaaag tattccagcc aagtttgtgg attcatattc gagacttttt tcattgataa
     1981 ataagaatca cagtggttct agtcgatatg aagtgagcca tgaagcagaa catgacaaaa
     2041 gaaaacagaa gggaacgttg acgttagtcg cgatcccttt ttttagtaaa agaaaagaag
     2101 gaatctaatt gatgaagaag atattgattt atttagttag aggatatcag cgctttattt
     2161 cgcctgcatt tccacctagc tgccgttatt acccaacctg ttccaattat atgataaaag
     2221 caattcaaac ccatggtgca ctaaaaggga cggtaatggg gactgcacgg atcttacgct
     2281 gtcatccttt tgtcaaaggc ggtatcgatt atgtaccatt gacattccaa ttaacgcgaa
     2341 ataagaaaga taaaaaagaa ccagattata aaaaggttgt gaaaaatacg agtagcactg
     2401 agcgataaag aaacaatttg gaaaatagct tcttatattt tgcaaaattg tgagttattg
     2461 ccgaagtgtt ggattttgaa caccgtcaaa aaaaggttgt gaaaaatacg ggtagcactg
     2521 agcgataaag aaaaagagga aaattattgt caaagtaaca actccgcaac tacttatatg
     2581 ggggacaaaa aatgatcgaa gaactaaaca agaaagattt accatggtcc ttgttattag
     2641 atgcagatcc tgataaagaa aaagttcaag tatatgtttc cagagggagt ggctggatat
     2701 ggaaagaaaa agaaaaaaca ataggtgtac tgatctatgt ggcaagggaa aaagaatacg
     2761 aaatcgtcaa tgtagcagta gccccaacgt atcaaggaaa ggggattggc ggaaaattac
     2821 ttgagacagc ttttcagaag ctttcacaac tcgcttccag tcaaactcag atcatcatac
     2881 gaaccggcag tactagcagt gcggcgttac atctctatca aaaaatgggc tatgtggaga
     2941 ttggtcgagt caaagactat tttatccatc attactctga gcccattttt gaaaatggag
     3001 atcaattaag ggatcaagta attttagcaa ttcacttacc taaaaatagc tttaactaat
     3061 aaactttcaa ttcgagaaaa tgctttcaaa tgctaaatag atacattaca atagctttag
     3121 caaacaaaaa ggagaatatt tttatgggaa tattagtttt tgattttggt ggatcggctg
     3181 tcaaatatgg ttgttgggat ggcaaagaga ttaaaggtaa gggacaattt gcaacgcctg
     3241 aatcttggga agaaatgaaa gcgcaattat ttcaagttta taaaaaaata ctcgtttctt
     3301 ttgaagtcga aggaattgga atcagctctc ctggagtagt gaataatgag aaacaggtga
     3361 ttgagggaat cagtgcgatt ccttatattc atggatttaa tattttccga gatttggaaa
     3421 ctctgtttca actcccagta acgattgaaa atgacgccaa ctgtgccgga atggctgaat
     3481 tttatgaagg tgctgcccgt gattatcaag atgttgcttt tgttgtggca ggaactggga
     3541 ttggcggggc gttgttcacg aaaggacaaa tcaataaagg ggcacatctt tatggtggag
     3601 aatttgggtt gatgttctta aatggcgata agacattcag taaacttggt acagcggtac
     3661 agatggcttg gcgttattgt gaacgcaaag gtctcgataa aaattcttat accggaaaag
     3721 atgtctttga attagcagaa actggagatg caatagcaaa agaagaagtt gagagttttt
     3781 atacgtatct tacaaaggga ttattcagta ttcaattttc gtttgatcca gaagtgatcg
     3841 tattaggcgg cggtgtttct gcaaaagaag ggctcattga tgagatcaaa agccgaatga
     3901 agaagctgac agaacaattc gatctgcatg atttcgaacc gcagatttta ttatgtgaat
     3961 acagaaacga tgctaatctt gtaggtgcag cagcaaacta tctcgctgta cgtcaagggg
     4021 agaattaaga gctttagaga aggtgcagct tgcataaaag aagtgaatga aatgaatagg
     4081 agatttttac ctaaggaatg ttttatttgc tgcagtatga taaaatatct gagaggtgaa
     4141 aatgatgtca aaaaaaaata aagatgttga agtgcgtata gaagaagtga aaaaagatat
     4201 caaaggtaag aattatgaag tcactcagtt atttataggg aaaaaaatga ttggtgagat
     4261 cttagcctat gggccaaaag aatatgaaat tttctttggt gaagaagatt ttggaaaaga
     4321 aaaaagtctt gaaaatgcga tagaaacagt gattcgacac tggaatctgc acgaataaaa
     4381 aaatatcaaa aaaactttca ataaagggtt gcaaaaatct ggaaaattag gtattataac
     4441 taagtcaggt tgatacaaac ctttcggagg agtagcgaag tggctaaacg cgacggactg
     4501 taaatccgtt ccttagggtt cagtggttcg aatccactct cctccatttc attggggtat
     4561 agccaagcgg taaggcaagg gactttgact ccctcatgcg ttggttcgaa tccagctacc
     4621 ccagtatacc acaatcgtaa gctctcgtta gttcgagagc tctttttttt gctttgctac
     4681 cttgttcctt gaagattctg ctgatttaag atagagttaa gttaactaca agaattggag
     4741 ggatccacat gaaatggaaa gaaaaagtag acgaagcagc tgaaaagtta tatgatttgg
     4801 tcaaatcaga aaaatacaat attgaagtga atattccgaa aaaaggcgga aaagcggtcc
     4861 aggtaaaatc aaaacgccca acgaatcata caaaaaaatg gatggctaaa aaccgctaat
     4921 aataacaaaa gatccataag tcggcatttt catcgatttt agggtctttt gttattttct
     4981 aggttgaaca tatctaaaaa attgtttctg acaataggag taggaagaac gatgtgtgtc
     5041 taaatcctct gttgatggat agaaaccttt atctttttca gttgtgaatc aggtatatac
     5101 cagttataat agaagaaacg aaaggacggg tgtacgttat gtcgaatcaa ttacctgtat
     5161 atatacagat tcatgaccaa ttaaaaaatg aaatcgaaca aggtatatgg aaagtcggag
     5221 accgtcttcc ttcagaaaga gaattagcag tgaaattcaa tgtcagccgt atgacgttga
     5281 gacaagcaat tcaaaacttg gcagatgaag gaatcttaga aagaaagatc ggttcgggta
     5341 catatgttgc gcgagaaaaa gtacaagaaa aaatgtctgg tgcaacaagt tttacagaaa
     5401 ttatggaatc gcaaaatcga gtaccgtcta gtcggacaat ctcttatttc ttgacttcgc
     5461 caagctcaag tgaaatggaa aaactaaact tgagtaaaga tgacacgatc ttgcgaatgg
     5521 aacgtattcg tttcgcagat gatgtcccta tttgttttga agttgccagt gtgccacaaa
     5581 agctgatcga tggctacagc aaagcagaga ttactgaatc gttgtatcgc acgctagaag
     5641 aaaaaggcgg gaaaaagatc ggtccagcga atcaaacgat tactgctgtg ttggcttctg
     5701 agaaaattgc tgactattta gaggtaaaac gaggagatgc agttcttcgt ttgcgccaga
     5761 tttcttattt tgaagacggt acgccgtttg agtatgtgcg tacgcaatac gtaggaaatc
     5821 gttttgagtt ttatttggaa aaataaaaaa caggtgatcc ctgattcctt acttagaaat
     5881 aaaagagaat cagaatcacc tgtttttata tatgatcagt taccagttgc tcgtgataag
     5941 aaacaggacc ctcttcaaat tggacttgtc cgttcagcag ctcaatgatg gattctttaa
     6001 agtggtcagc acttttttca tctacagcac agatgacgct aacttgatca gtatattgga
     6061 tttcttttat aaaaaaatgt tggagttcaa gataattctg taattttcct agttgcggat
     6121 acgtgatcat cacagagatc tcctgttgca aggtaccttc tacgattcca atgtcagaca
     6181 atgcttgaga gacagcatga gtgtatgctc gtattaatcc gcctgtgccg agtttcgttc
     6241 ctccaaaata tcgagtgact actgcagcta cattgatcag ttgatttttt ttgaggactt
     6301 ccaacatcgg tacgccagcc gttccacttg gttctccatc atcgctgctt cgctggattt
     6361 catttttttc tccaatgatg aaggcgctgc agttatggtt cgctttccaa tgttcttttt
     6421 ttatttcttg aatgaaactt tttgcttggt cctctgattc tattcgtcta agagaacaga
     6481 taaagcgtga ttttttgatc tcaatctcac tttgtccatt ttcttttatt gtacgatagc
     6541 gctcgatcat cttgtcacct ctttttttca ttttctttat tcagtataaa taaagttcta
     6601 ggttgtttca acactgtaac ggttgcgcaa tcgctgtagt atgcgataat aaagacaaag
     6661 gtaggtggat ctggggatgc aggaacgaat gaaaaagtac gatgcaatta cgcattattt
     6721 gaagaacaat ggcggctctc aagtaacgtt gacctttacc caatttgacg aactgctgtt
     6781 tcccagtaac ggacttccca aaacagcaag ggaaagtaca gattggtggg caaatgatta
     6841 taaacaccct gaaaaaggtg cctatggctg gataaatgca ggctatgaag tagttgtcat
     6901 caatttggac aaagaatatg tcgtgttcaa taaattagtg aaatctagtt ggctatttga
     6961 ttaaataaac cgcgtttaga aaacgttgac aaatttcttt ctttttatat aatagagtaa
     7021 cagacgcgaa gaaaacgttt gcgtagtttg ttttaaaaag gagagaaaag tatggctaaa
     7081 aaaacaatca tgcttgtatg ttcggctggt atgagcacaa gtttactagt aacaaaaatg
     7141 caaaaagctg ctgaagcaaa aggaatcgac tcggatattt tcgctgtgtc cgcttctgac
     7201 gcagataaca atcttgcaaa taaagatgta gatgtactgt tattaggacc tcaagttcgc
     7261 tttatgaaag ctgattttga aaaacgtctg gaaccaaaag gcattccttt agacgtcatc
     7321 aatatggctg attatggtat gatgaatggc gaaaaagtgt tagatcaagc aatcacgctc
     7381 ataggtgaat aaatagaaca gtataactat cgaacaagcg atccaaaaaa tagatcgctt
     7441 gttttttcgt atattttttt agggcgaata atatgaatca tttttggggt agaaggcttc
     7501 tgacgagaga tattaaagca atagactgtg aggaggtaaa tttaccaaat tatgagaaga
     7561 tcgcagcagt ggaacgtttg ggcaactgga ttctttgtca tcgctgtgga atgaggacgt
     7621 ccgtcattga tggacagctt gcagattacg gttatttttg tacacattgt ctatatttag
     7681 ggcgatgcga cactcaacaa gatttatatt tgtttgatca gcctgaagca aaacctcgag
     7741 aagtcgtctt tttttggaca ggacagctta ctgaaaaaca aacggaaatc gcaaagagaa
     7801 tactttgcca ttcagaaaaa agacatcatc ttatttgggc tgtaacaggt gcagggaaaa
     7861 cagagatgct ttatcctatt ttagtggaaa ctttaaaagc tggaggaaga gtagcgattt
     7921 gtacaccgcg agtcgatgtt tgtaatgaat tgtttttacg ctatcgttat gtttttccag
     7981 aagaaaaaat cacgttgctc catgggaata cagcaacggc ttataccttt acttcttttg
     8041 ttatctgtac gattcatcag ctataccgtt tttatcggtc ctttgattta atagtgatcg
     8101 atgagatcga tgcgtttcct tataacgggg atgcaggatt agctcatgct gtgcaaacag
     8161 cgattaaacc agacggacga ttcatctact taagtgccac acctgataaa aagttactaa
     8221 aggaaatcaa gcagacgttt gacatccaca aacttgcttt gcgctttcat cggcgtttac
     8281 ttcctgaacc cgtcttggtt ttcatgaata attggcgaca aaaatgcaag aacaagaaga
     8341 agataaaatc tttggtgcgg atcattcatc aattaattaa agaaaaccat atccttttat
     8401 tttgtccaag tatcacgatg atgcatcgtt tgaaagccac acttcaagag gctcttccga
     8461 gatatgagat cacagatgtt tcttcaagag acaatcagcg ggcggaaaaa gtccagaaaa
     8521 tgcgagaagc aaaatatgac atactgttaa ccacgatgat ccttgaacgt ggagtcacat
     8581 ttgaaagaat atccgttatc gttttaggag cggatcatcc ggtttttact aaatcttctt
     8641 tggtacaaat cgcaggaaga gccgatcgta aagggcctta tacgaacagt caagtatact
     8701 ttttctatga agaaaaaact gcagctattc gaaaagcttg tcaggaaatc aaagaaatga
     8761 atgaagaagg gaagaagatg ctgtgaggtg cagttgctgc agtaaagata tcgtccaaaa
     8821 tttaacattc agcgagatta ttggtgtttc gaaatacaga ttacctcaga cttgttcagt
     8881 ttgtcgcgca caatttcatt tattggaaac aagaggctgt tctggctgtc aaaaaccaat
     8941 ccatttcaat ctagagagta cgaaaaagaa agtaaagtct attcccgttt actgcagtga
     9001 ctgtcaagaa tggaagaaga aatatccgac ctatgatttt cagcaccgtg ccttttttgt
     9061 gtacgatgaa aatatgaagg cctggctcaa tcaatacaag tttatgggag atatccgact
     9121 agcaggcacc tttgcatata aatggacgca gataaaaaaa gaatacccga catacatcta
     9181 ttgtccaata cctttatctg aaaaaaggcg aacagaaaga ggattcaatc aagtaagcga
     9241 gatgttgaaa gcagcaagaa ttccttttca gatgctactc aaaagagaga agcatctgct
     9301 ggctcaagca aaaaacacgc gaaaggaaag attggagatg cctcaacctt ttgaacttgc
     9361 agtcaccttt tctaagatac aaaataaaaa aatattgatt attgatgatg tctatacaac
     9421 aggacagact cttttccacg cagcagattg tttgcaggtc gcacagccga aaatcatccg
     9481 aaccatttct ttagcaagat gaattagatg tggaaatttg atagcgattt aatttgcaaa
     9541 taacctagag ttcgatataa tagaattaag aagagggaaa gagtggtcct tctccttctt
     9601 gattgtggta gacgaaaggg gtaattttta tgtttagata taatgtacgc ggcgaaaata
     9661 tcgaagtaac tgaagcgatt cgcgactacg ttgagaaaaa agtaggcaaa ctagaacgct
     9721 atttcagtga cgcacccgat gcaacggcgc atgtgaactt aaaagtttat acagaaaaaa
     9781 cagcaaaagt ggaagtaacg atcccattac cttatcttgt attacgtgcc gaagaaactt
     9841 ctccagattt atacgcaagt atcgatttgg ttgttgacaa attggaacgc caaatccgca
     9901 aattcaaaac aaaaattaat cgtaaatccc gtgaaacggg catgaatact gcagatgcta
     9961 ctgctttatt caatgaagag gacgaaaacg aaaatgattc tgaattagat atcgtacgta
    10021 cgaaacgcct ttcattgaaa ccaatggata gtgaagaagc agtcttacaa atgaacatgc
    10081 tgggacataa cttctttatt tttgaagatg cagaaacaaa tggtacaagt atcgtttacc
    10141 gccgtaaaga tggaaaatac ggactgattg aaacagactg atcagcctta cttaaataac
    10201 aacgaaaact tgcagcagct actgacagct gccgcaagtt tttttgagtt tcctcctctt
    10261 ttagatgaaa tatgaataaa ttatgaagtt cttactttaa tacgcttaag atatttcaat
    10321 tatctataac taatgataaa atgaaaagta aagtctacat agtctggata atacacagaa
    10381 ggaaaaggag attacacaac gcatggccaa tttgattcgt tcacttatcg aaaacgataa
    10441 gaaagaacta aaacgtttag atagcattgc aaaaaaagtt gaagcatacg ctgaccaaat
    10501 ggcagcatta actgatgaac aattacaggc aaaaactgct gaattcaagg gcagatacca
    10561 aaaaggagaa acattagatc aattacttcc ggaagctttt gcagtcgtcc gagaagcagc
    10621 aaaacgtgta ttggggttat atccatacca cgtacaatta atgggtggga tcgtgcttca
    10681 cgatgggaat atccctgaaa tgcgtactgg tgaaggtaaa accttgactg caacgatgcc
    10741 ggtgtatctg aatgcgttga ctggcgaagg ggtccacgta gttaccgtca atgaatattt
    10801 agcaacccgt gactcgacgg aaatgggcga gctttataat ttcttaggat tgacagttgg
    10861 gttgaatatc aactcaaaat cttcggatga aaaacgcgag gcttataatt gcgatatcac
    10921 ttatagcacg aataatgaat taggatttga ctatttgaga gacaacatgg tcgtttaccg
    10981 tcatcaaatg gtacaacgtc cgctgaatta tgccattgtc gatgaagttg actcgatctt
    11041 gattgatgaa gcgagaacgc cgctgatcat ttcagggcaa gcagaaaaat caacagcttt
    11101 atatacaaga gcagacaact tcgtgaaacg tttgaaagaa gaagaagatt ataaaatcga
    11161 tatacaatca aaaacaatca gtttaactga agcagggatc gaaaaagctg aagaaacatt
    11221 cggtttggac aacttatatg acattgaaaa tactgcattg acccatcact tggatcaagc
    11281 attgagagcc aactacatca tgcttttaga catcgattat gttgttcaag atggaaaagt
    11341 attgattgtt gaccaattta cgggtcggat catggatgga cgccgttatt cagatggtct
    11401 tcatcaagcg atcgaagcta aagaaggcgt agagatcgaa gacgaaacga aaacgatggc
    11461 gacgatcact ttccagaact atttccgtat gtacaagaaa cttgcgggga tgactggtac
    11521 agctaagaca gaagaagaag agttcagaga gatttataat attcaagtat accagattcc
    11581 gacaaaccgt ccggtcatcc gtgacgatcg accagatttg ctttacccaa cattacaaag
    11641 caagttccat gctgtcgtac aagatatcaa agatcgctac cgcaaaggac agcctgtctt
    11701 agtcggaact gttgcagttg aaacatctga gttattatca gatatgctga atcaagaacg
    11761 tattcctcat gaagtactga atgcgaaaaa tcactttaaa gaagcagaaa tcatcatgaa
    11821 tgcgggtcaa aaaggtgcag tgacgattgc taccaacatg gctggtcggg gaacagatat
    11881 caagctaggg cttggcgtaa gagaacttgg tggcctagca gttatcggga cagaacgaca
    11941 tgaatctcgt cgtatcgata accagcttcg aggacgtgca ggacgtcaag gagaccctgg
    12001 tatgtcacaa ttctacttgt ctttagaaga tgatttgatg aaacgattcg gttcagaacg
    12061 aatcaaagct ttcttagatc gaatgaagat cggtgacgaa gatgctgtga tccaaagcaa
    12121 gatgctgacg aaacaagtag aagctgcaca aaaacgcgta gaaggaaaca actacgacac
    12181 acgtaagaac gtcttgcaat acgatgacgt gatgcgtgag caacgtgaag taatctataa
    12241 acagcgtcaa gaagtgatca tggaagaaaa aagcttatct aaaccactga tgaatatggt
    12301 caaacggaca atcagtcgtg tggtagatgc gcatacgcaa ttagaaaaag aaaattggaa
    12361 tttggaaagt atcgtcgatt ttgctggcag cacgcttgtc catgaagaca gtatttccct
    12421 tacagatatc gaaggaaaaa cctctcaaga aatcaaagaa tatctaaacg aacgagcaca
    12481 agaagtattc aataccaaag cagctcaatt gaacggacca gagcaactgc ttgaatttga
    12541 aaaagtcgtg atcttacgtg tcgtggactc taaatggaca gatcatattg atgcaatgga
    12601 ccaattacgt cagtcaatcg gcttgcgtgc ctatggtcaa aacaatccat tagtcgaata
    12661 ccaaacagaa ggttataaga tgttcgaaga tatggtaggt gccattgaat atgaagtcac
    12721 tcgtatcttc atgaaagctg agatccgtca aaacgtacaa cgtgagcaag tttctcaagg
    12781 aacagcttct cactcagaag atggagatgc caaagaaaac agtaatacta gcgcgaaaaa
    12841 acaaccaatc catgtgaaca aggttggtag aaatgatcct tgtccttgtg gaagcgggaa
    12901 aaaatataag aactgtcacg gaaaagatgc aaactaattt ctgacagcag gattggtgag
    12961 acaaattctt ttttaaagaa tttgtctcac cttctttctg ttatttgacg attctgttag
    13021 actagatata ataaaaaaag tatagtgaaa gaaggaaaaa aacatggaaa tatccgaaat
    13081 tcgaaatctc ctgacagaaa tgcaggaaaa ggtcacaagc ttcaggaggt ctctttgact
    13141 tagaccagct ggaagaagac attgcggaag cagaagcaaa aatggctgaa cctggttttt
    13201 gggatgatag cgaagctgct caaaaagtga tcagtgaaaa caatgcaaat aaagaaactt
    13261 atgatcaatt ccattctctt gctgaagaac tggaagaaat cgaattgatg gtggagatgt
    13321 tgcaggaaga acctgatgca gtaatgcaag aagaagcaga agcacgtatc aatgcgctag
    13381 atgaaaaaat gaatacgtat gaattatcta tgttgttaga tggtccttat gatcgaaaca
    13441 acgccatcgt cgaattgcat ccaggggctg gcggaacgga gtcccaagac tggggcagca
    13501 tgcttttacg catgtataca cgctgggcag agcagcatgg gtatcaagta gagacactag
    13561 attatcaagc aggcgatgaa gcaggaatca agagtgtgac cctactgatc aaaggacaca
    13621 acgcatttgg ctatctgaaa tcagaaaaag gtgttcatcg ccttgtacgt atctcgcctt
    13681 ttgactcagc aaaacgtcgt catacttctt tttgttcggt agatgtgatg ccagagcttg
    13741 atgaaacgat cgatatagaa atcaatccag atgatttgaa gatcgacaca tatcgggcga
    13801 gtggtgcagg tggtcagcac atcaataaga cagaatccgc tgtacgaatc actcacttgc
    13861 ctacaggaac agtagtagcg agtcaggcac aacgttcaca attgaaaaac cgggaacaag
    13921 cgatggggat gctgaaagct aagctttacc aattagaagt ggaaaagaaa gaacaagaag
    13981 cagctgcact gagaggagaa caaaaagaaa tcggctgggg atcacagatc cgttcttatg
    14041 ttttccatcc atattctatg gtaaaagacc atcggacaaa cgtagagaca ggaaatgtcc
    14101 aagctgttat ggacggagat cttgatttat ttattgacgg ctatttgaaa atgcatctgc
    14161 aataagagcc ctttgttaaa ttgaaataaa attgtaagat tttgcaagca agttgaaaca
    14221 ttgccatgct ataatagcta aagactgaaa aagatatgga gagaacgcca tgattgaaat
    14281 gaaggatgta atgaagaagt actcaaacgg taccactgct atccgcaatc tctcgattgt
    14341 catcgaacaa ggagagtttg tgtatgttgt tggtccgtct ggagctggta aatctacatt
    14401 catcaaattg atgtatcgag aagaaaaagc ttcaaaagga actttgaacg tcgcaggtca
    14461 tgatctaata gccatcaaaa accgcgaagt tccttatcta cgcagagaaa tcggcgtggt
    14521 gttccaagac tataaattat taccaagaaa aacagtatat gaaaatgttg cgtacgccat
    14581 gcaagttatc ggcagaaaac ctcgggatat caaaaaacga gtaatggaag tgctggatct
    14641 tgttggtttg aaacacaaag tccgagtctt tcctagcgaa ttatctggtg gggaacaaca
    14701 acgtgtttct attgcccgag caatcgtaaa tacacctaaa gtacttatcg cggatgaacc
    14761 gacagggaat ctagatccgg aaaactcatg ggaaatcatg aaactattag atcggatcaa
    14821 tgcacaagga acaactgtcg tgatggctac acataacagt acgatcgtaa atacgattcg
    14881 acaccgagta attgctatcg aaaacggccg cattattcga gaccaagcgg aaggggaata
    14941 tggatacgat gattagaacc tttttccgcc atttattaga aagtatcaag agtttgaaac
    15001 gtaacggctg gatgacagta gcttcggtca gtgcggtaac gatcacgttg actttagttg
    15061 gtgtgttcat ggcggtcatc atgaacgcaa ccaaactagc acaagatata gaaggaaacg
    15121 tggatgtatc tgtgttcgta gacattggta caaaagacga agagatgaaa aaattagaaa
    15181 aagaattaaa tgacttagat catgtcaaaa aagtcgaatt ttcaagcaaa gaacaggaat
    15241 tgaagaaaat ccaaaacgaa atgggcgatt cttggaaatt gtttgaaggg gataataacc
    15301 cgttatatga tgtatacgta gtaagtgcta ccgatccttc ttatacaaaa caagtagcag
    15361 aaaaagcggc agatttgaag aatgtttccc gttctgatta cggtggggct tcctcagatc
    15421 ggattttcca aattgctggt gcagttcgaa catggggatt agttgcggcg gcattgctgc
    15481 tgtttgtagc aatgtttctc atttctaaca cgattcggat cacgatttta tctagacagc
    15541 gtgaaattca aatcatgcgt ttagtagggg caaaaaatgg atatatccgc tggccatttt
    15601 tccttgaagg tggttggatc ggcttaatcg gtgccattct tccgatactg atcattacat
    15661 ttggttacgc atggttttac caattgatca atccgtcatt attacgatct cattattcat
    15721 tgatccatcc agaaaatatc gtttggaaaa tcaatctttt gatggtcggt attggtgtga
    15781 tcattggttc actcggctcg atcatctcta tgagacgttt cttgaaattc tagtgtcaca
    15841 agataaatat aatcagaaag ttggacgatt tcgttccagc tttctttttt tatcagctag
    15901 gtggagtaaa tggggcggct tttttctctt tactttaaaa aacaacttgg gtattcttaa
    15961 gttggtagaa aaagaggtga agccgtgaag attatcgttg acaatttttt tacgatcctt
    16021 ttgattatga atattttgct ttcctttatt atcgtttttc gagaaagaaa ggaaactgcg
    16081 caaacatggg catggctgct cgttttgatg ttcattccag ttgtaggatt tattttatat
    16141 atttttttag gaagagggat ttccaaagag aaaatctttg atttgaagat ccaagggaaa
    16201 atcggtaaga atctagaaat cgaagaagat aaacaagcat tgatgcgagg gctttatcct
    16261 catccgccta ctggacaagt agatgtgaaa caattgatct atatgcttac tgtatttgaa
    16321 agtacgcttt atacgactaa aaatgaaatc actttattta cagatggtcg tgaaaaattc
    16381 gatgcgctga tcgaggatat caaacaggca gaagaccata tccatttcca atattatatt
    16441 taccgcagtg atacattagg tgaagaagtt cgagaggcat tgacagaggc agcaagacgc
    16501 ggagtaaaag tcagagtcct tcttgatgca tggggttcca cacaagtttc ttctagtttt
    16561 tttcagaatt tgaaaaaagc aggtggagaa gtcgcatttt tctttcctct attcgttccg
    16621 tatatcaatc ctcgaatcaa ttatcgcaat catcgtaaaa tcgtagtgat tgatgggaaa
    16681 atcggatata caggtgggtt caatgttgga aacgaatatc taggattggt aaagaaattt
    16741 ggttattggc gagacaatca tctgcgtatc tatggagaag cggtttacac cttgcaaaac
    16801 cgttttttga tggactggaa ttctcagcat acaaaagaag taggatattc accggaattt
    16861 ttcccgacca ttcattctac aggtgatatg gctgttcaaa tcgtgacaag cggaccagat
    16921 accgaacatg agcaaatcaa aatgacatat ttgaaaatga tttcgttagc caaacgggag
    16981 atattgatcc aaacgccgta ttacataccg gatggatcga ttcatgaggc attgaagttg
    17041 gctcttctct caggcgtaaa agtccatatc caaataccta acaaaccaga tcatcttctc
    17101 gtttactggg caacgtattc tttcgctgca gagctgatcg agtatggggc aagaatcgaa
    17161 acatatgaaa atggatttat ccatgcaaag acgatgatta tcgatggcgg catcgtttcc
    17221 gttggttcag ctaatatcga cgttcgctca tttagattgg attttgaagt caatacactg
    17281 atctatgatg aacgaatggc ttctcgcgta agaaaagcat tctttgaaga ttcaaagatt
    17341 tcgacgcatc tcacaaaaga agtgtatgaa aaccgaggga tgatgatcaa gatgaaagaa
    17401 ggattagcaa gactgatttc gccgctcctt taaaatacaa aaaggacagg agagccatca
    17461 atagatggaa atctcgcgtc cttttttctc ggggccattt ctgagagtac gtcagaaatg
    17521 gagaaagcgt ctcgcatgag cgaaattaac tttggggtat ctcttgtttt ttggggaaag
    17581 caagaaataa gctaatatat gctattaaaa gagacacatt tcaaagccag tatagcatag
    17641 aaacttttaa tgagatagct ttttttgcaa ttatgagaat ttattttgtt atttaatgat
    17701 tttgattcgt tttcatcaag taatgaaaat gatagaataa acatacttta ggattaaagg
    17761 agatttctaa tgaaaaaact aaaattattc ttaccaataa taggattagc tattctttta
    17821 tcaggctgtt caaagtggat tgatcgagga gaatccatta ctgcagttgg ctcttctgcc
    17881 ttacagcctt tagtagaaac ggttgctgaa acctaccagg cagaaaatcc tggaaaattc
    17941 atcaatgtac aagggggcgg aagcggaact gggttgtctc aagtacagtc aggagcagtc
    18001 gatatcggaa attctgattt gttcgcggaa gaaaaatcag ggattgatgc ttcggcatta
    18061 gtcgatcatc gagtagcagt tgttggaatc actcctattg tgaacaagga tattggcgta
    18121 aaagatatca gtatggaaaa cctgaaaaaa ttatttacgg gcgagatcaa aaactggagt
    18181 gaagtcggtg ggaaagatca gccagtagtt atattgaacc gtgcatcagg aagcgggacc
    18241 cgtagtacgt ttgaaaaatg ggtattggac ggagaaacag ctgttcaggc acaagaacaa
    18301 gattcaagcg gaatggtgcg ccaaatcgtt gcggacacac ccggagcaat cagctatgtt
    18361 gctttttctt acgtttccga tgatgtggat acgttgaacg ttaacggtgt ggaaccaaca
    18421 gatgaaaatg tgacaacgaa caaatggacg atttgggcat atgagcatat gtacacaaga
    18481 gaccatccaa aaaaactgac aaaagaattt ttagactata tcttatctga tgaagtacaa
    18541 gataatattg tcggagagct aggctatatc cctgtttcac atatgaaagt tgaacgtgat
    18601 tggcaaggaa acattgttaa agagtaatct ttacacaaaa tttacaaact tagctaaact
    18661 tttacatgtt attaaaacca aattgatgtt caatttgtac actgagtgag tatgttaaac
    18721 ctgattaagg gggagtcatc tgtggaagat gtgaaaaaag tattattaac aaaatcaaaa
    18781 cgttcaaaaa tggaacagcg cggaaaattc attagttttt tgtgtatcgc gctgatcgtg
    18841 ttggttgtat tatctatttt ttattttgtc gctagtaaag gattggccac cttttttact
    18901 gataagatca atgtatttga ttttctgttt ggtacagatt ggaatccaag ccaaatagga
    18961 gcaaatggga aacccatggt aggggcattg cctatgatca caggttcatt tattgtgacc
    19021 tttttatctg ctattgttgc cacaccattt gctattggag cagcagtgtt catgacggag
    19081 atttctccta aaaacggaac gaagatcttg caaccggtca tcgagctact tgtaggtatt
    19141 ccgtctgtcg tttacggatt tatcggttta tctgttatcg tgccttttgt cagaagtatc
    19201 tttggcggga cagggttcgg tatcttagct ggtacatttg tgttattcgt tatgatctta
    19261 ccgactgtca caacgatgac tgtggatgca ttaaaggcag tgccgcgaca ctatcgggaa
    19321 gcttcattag cgctaggtgc cacaagatgg caaacaatct acaaagtggt gctgcgagca
    19381 gcagtgcccg gaattttaac agcagtcgtt tttggtatgg ctcgtgcgtt tggcgaagcc
    19441 ttagctatcc aaatggttat tggtaatgcg gcattaatgc caacaggact tactactcct
    19501 gcttctacat tgacgtccat cttgacaatg ggaatcggaa atacaatcat gggaacagtt
    19561 gaaaataatg tgttatggtc attagcgctg atcttgctat tgatgtcatt attctttaat
    19621 atcgtgatcc gtatgattgg gaagaaagga gagctgaaat aatgaacgca aaacgctcag
    19681 ataaaattgc tacaggtgtt ctatatgtgg tttcagggat catcgtattg atcttagcag
    19741 ctttattact ttatatctta gttcgaggaa taccgcacat ttcatggtca ttcttgactg
    19801 aaccttccaa agcctaccaa gaaggcggtg ggatcgggat tcaattattt aactcgattt
    19861 atttgctttt gattaccatg atcatcagtt ttcctatctc tttaggtgcg ggtatctacc
    19921 tttcggaata tgccggaaaa aactggctga cagacgtgat ccgtacgtct atcgaaatct
    19981 tgagctcttt gccttctgtt gttgtcggtt tattcggttt cttggttttc gttattcaat
    20041 tccagtatgg tttctcaatc atttctggtg cattagcttt gaccgtattc aacttgcctc
    20101 ttttgactcg aacggtcgag gaatctttac aagcggtcca ttacacacaa cgagaagctg
    20161 gattggcgtt aggtttgtca cgctgggaaa cagtgataaa agtggttgtt ccagaagcga
    20221 caccaggaat tttgactggt gtgatcctaa gttcaggaag aatttttggt gaagctgccg
    20281 cattgattta tacagctggt caaagcgcgc cagcattaga ttttacgaac tggaatccat
    20341 taagtgtttc tagtccgatc agcattttcc gccaagctga aacactggct gtccatattt
    20401 ggaaaatcaa cacagaaggt acgatgcctg atggtgtttc tgtttcggct ggcgcttctg
    20461 ccgtgttgat cattgctgtt ttactgttca acttcggcgc tcgtgcgatt ggcaaacgat
    20521 tataccgaaa aatgacttca gcctaaggag aagaagtaaa atgaaaaaat ataattggaa
    20581 tgaaacaaat gtcattacca tgccaaaaga taaggaagtt gcattatata cgaatgatct
    20641 gcatgtttgg tatggcgaca atgaagcaat caagggagtg gatctagaat tcgaaaaaaa
    20701 taagatcact gcattgattg gtccctctgg ctgcggaaaa tccacgtatc ttcgttcttt
    20761 gaatcgtatg aatgatggga ttgctaatac gaaaattact ggaaagatta tgtataaaga
    20821 cgtggatatc aatgcgcctc aagtggatgt ttatgaaatg cgtaaacgga tcggcatggt
    20881 gttccaacgc cccaaccctt ttagcaaatc gatttatgac aatataactt ttgctttgaa
    20941 acagcatggt gaaaaaaaca aaaagaaatt agatgaaatc gttgaaacaa gcttgaaaca
    21001 agcagctttg tgggatcaag taaaagatag cttggataaa agtgccttgg ctttatctgg
    21061 cggacaacag caacgtttgt gtatcgctcg tgcaatcgcg atgaaacccg atatcttgct
    21121 gttagacgag ccggcaagtg ccttggatcc tatttcgaca ggaacggttg aagaaacact
    21181 tgtaaacttg aaagaagact acacgatcat tatcgtgacg cataatatgc aacaagcagc
    21241 tcgtatcagc gactatactg cctttttcta tctaggaaaa gtaatggaat atgacaaaac
    21301 aaacaagata tttaccagac caaaaattca ggcaaccgaa gattatgtgt ccggtcattt
    21361 tggttaagac ggaggcctca acatgacaaa agaaattatc tcatccaaag atcttcatct
    21421 gtattatgga aagaaagaag cactaaaggg aattgatcta agtttcaatc aaggtgaaat
    21481 cacagcgatg atcggtcctt ctggttgcgg gaaatctacc tatctacgtt cgttgaatcg
    21541 aatgaacgat ctgattccaa atgtaacgat caccggaagt gttttatata aagacaaaga
    21601 catctatggt ccgaaaacag atgttgttga actaagaaaa gaaatcggca tggtgttcca
    21661 acagccaaat ccatttcctt tttctattta tgaaaatgtc atctatggat tgaagttaaa
    21721 aggtgaaaaa gataaaaaag tccttgatca agtagtggaa gaaagtctga aggcagcgtc
    21781 cgtttgggag gatgtgaaag ataaactgca taaaagtgcc ttgtctttat ccggtgggca
    21841 gcagcagcgt gtatgtatcg cacgtgtttt ggcagtgaat cccgaaatca tcttattgga
    21901 cgaaccaaca agtgcacttg accctgtttc tactgggaaa atcgaaagta tgctgttaga
    21961 attaagagag cggtatacga tgatcatcgt gacccataat atgtctcaag cttcacgtat
    22021 ttcagataag acggcattct ttttagatgg tcacttgatc gaatacaacg atacgaagaa
    22081 gatattcatg aatccagaaa aacaagagac agaagattat atttcaggtc gatttggata
    22141 aggaggaaac ggaatgctac gtacacaatt tgaagaagaa ttgctgaatc tccataatca
    22201 gttctacgaa atggggatga tggtgagcag cgctgtgcac aagtcagttc gtgcatacat
    22261 caaacacgat aaaatcttag ctcaagaagt tatcgaaaac gatataaata tcaataatat
    22321 ggaaacgcgt ttggagaaga aaagttttga aatgattgca ttacaacagc cagttacaac
    22381 tgacttgcgt atgattatca cggtcatgaa agctagctct gatttagaac gtatggccga
    22441 tcatgcggtg tccgttgcta aatccacaat ccgcgtaaaa ggacagacga gaataccgga
    22501 aatcgaaaaa gagatctccg atatgtccga ttacgtgaaa aaaatggtgg acaacgtcct
    22561 agttgcgtat gtcaaaacag acgaaaaaga tgcgcgtaca atcgccaata tggatcaaag
    22621 agtcaatgac tatttcaatc gaatctataa tgcaaccatc aaaaacatgc aagaaaatcc
    22681 tgaaacagtc atcagtggca cagactattt gaatgttgcc agctatctag aacgaatcgg
    22741 agactatgtg acaaacatct gtgaatggat cgtctacttg gctacaggga aaatcactga
    22801 attaaatacg aaccacaatg agattttcta aactgaagtt gtgattctgc tgtttattta
    22861 ggaaaagagg ttgtgaaatc agcgttttgt cctgagtatt agagaaacaa acggtaaaat
    22921 agttcctcat attttgccgt t
//
LOCUS       DACGLW010000045        21408 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.045, whole genome shotgun sequence.
ACCESSION   DACGLW010000045 DACGLW010000000
VERSION     DACGLW010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.045"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            2..400
                     /locus_tag="IXT53_001710"
     CDS             2..400
                     /locus_tag="IXT53_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HAQ4905532.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            384..1079
                     /locus_tag="IXT53_001711"
     CDS             384..1079
                     /locus_tag="IXT53_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HAQ4905533.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(1673..2560)
                     /locus_tag="IXT53_001712"
     CDS             complement(1673..2560)
                     /locus_tag="IXT53_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rotamase"
                     /protein_id="HAQ4905534.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            2745..3584
                     /locus_tag="IXT53_001713"
     CDS             2745..3584
                     /locus_tag="IXT53_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ4905535.1"
                     /translation="MIRIIRLIGSVLIVFVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            3780..4754
                     /gene="sgrA"
                     /locus_tag="IXT53_001714"
     CDS             3780..4754
                     /gene="sgrA"
                     /locus_tag="IXT53_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HAQ4905536.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            5052..5408
                     /locus_tag="IXT53_001715"
     CDS             5052..5408
                     /locus_tag="IXT53_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905537.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            5411..6568
                     /locus_tag="IXT53_001716"
     CDS             5411..6568
                     /locus_tag="IXT53_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905538.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6594..7034)
                     /locus_tag="IXT53_001717"
     CDS             complement(6594..7034)
                     /locus_tag="IXT53_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ4905539.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            7194..7700
                     /locus_tag="IXT53_001718"
     CDS             7194..7700
                     /locus_tag="IXT53_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ4905540.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKNHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            7868..9781
                     /locus_tag="IXT53_001719"
     CDS             7868..9781
                     /locus_tag="IXT53_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HAQ4905541.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            9786..10787
                     /locus_tag="IXT53_001720"
     CDS             9786..10787
                     /locus_tag="IXT53_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ4905542.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(10989..11195)
                     /locus_tag="IXT53_001721"
     CDS             complement(10989..11195)
                     /locus_tag="IXT53_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HAQ4905543.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(11841..12689)
                     /locus_tag="IXT53_001722"
     CDS             complement(11841..12689)
                     /locus_tag="IXT53_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ4905544.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            12853..13755
                     /gene="gnd"
                     /locus_tag="IXT53_001723"
     CDS             12853..13755
                     /gene="gnd"
                     /locus_tag="IXT53_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HAQ4905545.1"
                     /translation="MEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDI
                     PAYQEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSI
                     RRAEEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLY
                     AGASGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWL
                     MEIMEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEED
                     SFSAKVVSALRNGFGGHEIVEKGE"
     gene            13757..14758
                     /locus_tag="IXT53_001724"
     CDS             13757..14758
                     /locus_tag="IXT53_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HAQ4905546.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            14755..15039
                     /locus_tag="IXT53_001725"
     CDS             14755..15039
                     /locus_tag="IXT53_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ4905547.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            15072..15869
                     /locus_tag="IXT53_001726"
     CDS             15072..15869
                     /locus_tag="IXT53_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ4905548.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            15883..16716
                     /locus_tag="IXT53_001727"
     CDS             15883..16716
                     /locus_tag="IXT53_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ4905549.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            16734..17591
                     /locus_tag="IXT53_001728"
     CDS             16734..17591
                     /locus_tag="IXT53_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ4905550.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            17613..18122
                     /locus_tag="IXT53_001729"
     CDS             17613..18122
                     /locus_tag="IXT53_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ4905551.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            18159..18560
                     /locus_tag="IXT53_001730"
     CDS             18159..18560
                     /locus_tag="IXT53_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905552.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            18672..19025
                     /locus_tag="IXT53_001731"
     CDS             18672..19025
                     /locus_tag="IXT53_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905553.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            19059..19502
                     /locus_tag="IXT53_001732"
     CDS             19059..19502
                     /locus_tag="IXT53_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAQ4905554.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            19519..20604
                     /gene="uxuA"
                     /locus_tag="IXT53_001733"
     CDS             19519..20604
                     /gene="uxuA"
                     /locus_tag="IXT53_001733"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAQ4905555.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            20597..21310
                     /locus_tag="IXT53_001734"
     CDS             20597..21310
                     /locus_tag="IXT53_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ4905556.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
BASE COUNT         7364 a         3285 c         4469 g         6290 t
ORIGIN      
        1 gatgacgatc tttgaaacgg ttgtaacaga tgttggtata gaagcagaag tattcaggaa
       61 agaaaaaata atgattttat ttggagatga agcaccggaa acattaacag attattgtta
      121 taagattgaa ttgaatcagg taagaaaaga aataacttgt gatatgaagc tctgttttga
      181 taaacaaatg tatgtaatca cagcagtagg agatgttgtc caaactaatt tgaatgaatt
      241 aggacatatc acgataaaat ttgatggatc tcatgagccg gaacttccag gaacaatgta
      301 tgtagaagat aaggaaatgc cgcatttaag aaaggggaca atcattaaaa tcgatcaaga
      361 ggatcagaat gggggaaagc tatatggaaa tcatgcttga tacagcaaat acaaaaagta
      421 tttattcttg tcaacagttt ttgaatttaa caggcgtaac aacaaatcct acaattctga
      481 aaaaagaagg agcgtcaaac ttcttctcgc atatgaaaga aatcaaaaaa ataatcggac
      541 agattcctat ccatattcag acaataggaa gaacggaaga agaaatgata gaggatgctc
      601 gagttatcat agaagaactg ggaaaagata cgtttatcaa ggtgccggtg aatcaagcag
      661 gccttcaagc gatcaaaaag ttgaaaaaag aaggttattt gattaccggt acagctattt
      721 acacagaatt acaagggtat ttagctatta ataatggagc agattatatt gcaccttatt
      781 ataacaggat gcaaaatcaa ggcattgatg ctatgaaagt gattgattcg ttgcggactg
      841 agatcgaacg tacgcaaaga ccaacaaaaa tattagcagc tagtttcaaa aattgtgaac
      901 aagtcactac agccattcga tctggagctc atgcgataac gttaggtaaa gagctactag
      961 atcaagtatt agagatgccc tcgattgata aagcagtgag gggcttccaa gaagattggg
     1021 tgcaaacttt ccaagcagaa acgattggtg aactaaaaaa ggtcgctgat tacttctgaa
     1081 tcaatttctc cataaaataa acgatagcct tctcgaaaac tcgaggaggc tatctgacga
     1141 ttcaatattc attaatttaa agtttgggtg actattacta actattactt atttctcatt
     1201 taaacgatgt attccctaac ctctaataca tccttgtaac tctgaagtta cgccagtttt
     1261 gaataaattt cataaaaaca aatgaaaagt gtcgcataaa aatatctttc gtgtttctct
     1321 agtataacag aataagaaga acaagtccag aagggatatt tttagtcttt tttaaaataa
     1381 cttttcagtc gagtttgagt ttatgatagt tgtttttgaa gcgatacggc aagtgaaaaa
     1441 atagggaagc atgaaagaat ctacttaatg gcgcattgat tcatgtgata gaaataaaaa
     1501 acttttttaa attatatcct catgattgta acaaatccag tgatttgttt tggtacaaat
     1561 aaaaaaaggc cccgatgaag ttgtggcaaa agtggtttgc ctcaaaaata agaaggaatt
     1621 tccaaaaatc acttctcaaa ttttgccaca tccccaattg ggtcaaattt gtttatttga
     1681 atatattttt tacataagga tctttaatag ttacattgtc ttttttcatc actttacgga
     1741 ttgttttatc catgaattcc gtatcaagag tttttgcttc ggtagcaatt ttttctagct
     1801 cagatttgta tttgtcttta ttagaacctt tatcctgttt ttttaccatt tttactagat
     1861 aatagctttg ctgataagtc gttggatctg ttacaggaat gatatcagaa acttccccgt
     1921 tttttagttt aaatgctgct ttttttactt cagtaggaat ctctggattg gttgaatcaa
     1981 aattcatttt tccatcagtt tcctttaatg ttttattttt tccataagct tgaacaagct
     2041 tggagaaatt ttctccttca tttgcttctt tttttgcttt atctgcttct tctttagtcg
     2101 aaaatagaat gatctgtgta ctaacttctg gatagaattc tttccaagca gtgtctagat
     2161 ctttatctgt taattttaca ttggctttca aaccgtattg gaaagccagc atctgtttaa
     2221 agagtttctt atatgtctct tctgtttggc caactgcttt taattgttcc ttgtagtttg
     2281 gaccgagttt tttgatttgt tcattatatt cttttgttac ttcctttgaa gaaatctctt
     2341 ttccgtatac atcatcaaat gctttgaaga caatcatttt ttttaatagt gtttgattat
     2401 ttgatttaac ttttgggtta tcataaaaat cctgaactgt aagtgttcca cctttaaacg
     2461 ttgcaatcgt ttcattggac ttgctacaac ctgagaatag taaaattgca catgctgaaa
     2521 ggcacataat tagtagttgt tttttcaaaa aatttcccat cctttcctta ttaaaaacag
     2581 tctaacagaa gaattaggga gacagtcttt cattttcgta aggttgctgt agatattctt
     2641 agttcgtaaa aatctattct ctctatgatg gagaacattt acatagtttt cacgagacaa
     2701 gaaagaaaag ggttgttaaa cttaatataa aggagggatg aaagatgatc aggattattc
     2761 ggttgattgg atctgtttta atagtctttg ttggttttgt tttaggaatt cttgtcaata
     2821 atgcacttgc ggatatgttt attggaaata gtgaatggat tggagcaatt gccggtacgg
     2881 tcggacattt agctgttttt ttactagctt tatttcttgc tagtaaggtc gaaggaaaaa
     2941 gaatagaaga ttatggtgtt tctgttcgag gaaaagactg ggggtattta gcgggaggtc
     3001 tcgttgttgg aattggagtg tttctgctta ttactagtcc attatatttg acgggggcgt
     3061 atcggttaga ttcaggaaac gcaaacattg tgccgctgat aacgagtttt attttattta
     3121 ttgcagtggg ggcatcagaa gagcttttat tccgtggctt ttttcagcat cagatgctta
     3181 gatttggtcc gttaatagca atgattggat ctgctgcatt atttgctctt ttgcacggat
     3241 tgaatcctaa catgacattt ctggcagttt tcaatatctt tttagcaggt tgctttttca
     3301 gcgcacttat ctacagtaca ggaagtttat ttacggctat tggtgctcat attacttgga
     3361 attggacaca aggagctatt ttgggtatac cagtcagcgg aacgcaagaa caaggttttt
     3421 tctccactct gatccaatcg caaaatacaa taattactgg gggaaacttt ggggctgaag
     3481 catctatatc aactacatta ttcttgttag tcttgattgt tggtttgtta ctctatgcat
     3541 ataaaacaaa gcggatgaag aaatacactg ctgtttatca ataaaagatg aagaaagcaa
     3601 ggctgatatc ggtcttgctt ttttaagtct gtcattgatt aaacgaaaaa gtaaacaaat
     3661 tcaagcgaat acacgacatc ttttgttaga ttttcacaac tgactgtcgt gttctcatca
     3721 ttttcatttt atataattcc tttatcaaat agaaggtaat tatatggaga gtgatagaaa
     3781 tgaagaaaac agcgaccgta ttatttgtta ttcttgggct gagtataatt gcagcaatga
     3841 ttggaatcag tacttctgca aatgaacggg caaatgagaa tctttcattt actgtgaaaa
     3901 ctgatcggat tgtttatgac atgacacaac aagtcattac tattccagtc aaaccaaaca
     3961 aaagcgtgaa tgctagtgat gtacatgctg ttctaacata tggttgggat ggtaatggaa
     4021 gttcagaaaa agttattggg gaagtttatt taaaagacgt acaatggacc gctggtattg
     4081 agtatacaat aatgatttct gcagaactat cgattgatga gatcaagagt aaagataaag
     4141 ttgatttgat tgttttttat gatggacaga tgacgatcac ggagaatttg aaaccaagca
     4201 gttggacagt tgttggaccg ccaagcagta cggatagcac aagtagtgaa agcagtacag
     4261 aaaacacgag cggtgaaagc agtacagaga gtacgagcag tgaaagtagc acagaaaaca
     4321 cgagcagcga aagcagtacg gagagtacga gtagtgaaag tagcacagga agcacgagca
     4381 gcgaaagcag tacggagagt acgagtagtg aaagtagcac aggaagcatg agtagtgaga
     4441 gtagcacagg aagcacgagc agcgaaagca gcacaggaaa agtaaataat gaacttgatt
     4501 tggtacctat cataccaact aaggaacaaa agagaaatgc agggagcaac ctaaaaagtg
     4561 tgctaaaaca gcctgatttt gtttctcgtg gaattgatca tcaagaaaaa gcgaaagaaa
     4621 cagataatag aaaaagcgat ttaccagaaa ctggtagtag aaccttgaac aagctaatta
     4681 catggatggg tgtgttactc attttgattg taggagcaag ctattttcgt tctctcttcc
     4741 atcgagtgaa ataaagaata aaagcaacag atctaaaatt gaataaaacg atagattttt
     4801 gagtgaatat ttcattcgag agtgctgtat tgcctggcga agttgtagct ttgccagatc
     4861 tatttttgtt tactaacata tttgagaaac aggaaataac aggaacacaa atggaaaata
     4921 agagcaagaa taattgacaa tgcacactgc gcatggtagt atctctgaaa aatcagtaac
     4981 gaatctattt gtacgttatt catgtagctg tcggaataag tctaaataat agataaaaag
     5041 gagtatgtac tttgtttgaa gaatttattg atatcaatga aaggcaagta tatcaatttt
     5101 tgaactattg ttatgaaaga gacgagaaat tatatgtggt gaaggatatc gctttagatc
     5161 taaactacac cctggctaag atgaattcgg tgattcaaca agcagaatcg ttttgcgaaa
     5221 ggtatccaga atataaatta tcttttcttt cagaaaacaa gatgataaaa gtcgagtttt
     5281 cttctcagtt tttactgagt aaagtttatt ctatactttt agagggaacg attggttata
     5341 ttttactgga ttcactttac aaaggtactt atcaatcctt agaaaatctt agtcaaaaaa
     5401 ttatataagt ttgcgtacga ttcaaagaaa attaagagaa cttaatacta ttttgaaaaa
     5461 ctatcattta tcttgcagct tgaaaaaagg taatccgata gaaggagaag aataccgtat
     5521 ccgatacttt tttcacttga tgtattggca gtcattcgac gaaaatcatt ctgatatatt
     5581 atcaagtata aaaaataaac gtcaactatt agaaaaattt gcgaaacatg cgccatatac
     5641 gcgtagtata gatagaaaga aatggatcag cctactaaga atttcgttgc aacgaatgaa
     5701 acaaggccac tcgattacgt atgtcccaga agagatgaac aaatttgtgc atccattgat
     5761 aacaaaagaa gagtttacgc attacatact agagcctttt tttgaagcaa acttctggtc
     5821 gatgaatcac atcactccaa cagagcaagc ttacctttat tttatgtttg gtgtaatgaa
     5881 cacctatttg gaagaagatt tgttggatac tccagataaa gtacaagaaa gttggggctg
     5941 gattgaatcg tctattgtag aaaattttga aaaaaacttc aagatttgtt ttacaacttc
     6001 agaaaaaaat tatctatatg tcaatttggc aatgatccat ttgtattcaa gggtttttgg
     6061 gacaaaaaag aaggtggatg cttttggaaa atcagcagaa gacagagatt ttcagcatat
     6121 atttccagtg atgtatccaa tcatgcaaca gttttttagt ggggtagctc gcagaattcc
     6181 agagcttaat acttattatg aaaagaacag acggttgatt tttcaatact gtatgttagt
     6241 cagggatatt tttataaaac acgaacaacc tgtcatattc tatattcaat caaagtatgg
     6301 taaaacacag gaattatggg caaagaaaag gatattatct atgactgaac gcccaataaa
     6361 atatagtgca tccgctgatc agttacctga tattgtcatt agcgattatc ctatcgacaa
     6421 aaaggcatat gatcaaacga atacacagat attttactgg aatggacaac ctacaaaaaa
     6481 tgattgggcg cgtctgaaag ctgaaatcat aaaaatcagg aatgagaaaa atagatccat
     6541 attggaaaaa ttggatttcg ttaattaaga aaagcagatt gtttatgcca aaattaagac
     6601 attgcaaaaa gattattttc tttaaaatcg acaagatgac cagaaagcaa gagttcgttg
     6661 agctcttgct ttcttttagt tttagaaagg aacatcctga ttttatcttc gctaaatcca
     6721 gtcaaaagtt ttgcagattg gtcgtagata atcggttttt tcaataaaga aggggatgta
     6781 cgaatctcaa tatatagttc actcaaactg agttcatcta ttctgtgaac aagattttta
     6841 taactaatag aatgagtaga gagcatttcg gtcacgccgt tgtttgtcaa ttttagtaag
     6901 tggataaatt catttttggt tagtggttga cgaattaagt tctgttcgat aaacggtata
     6961 tcatggacga taagccaacg ttttgcgcgt cttgaagacg agcagccaga acctgtatac
     7021 aatttaatca tcatacattc ctccaatagc taagtattta tgaaataata acatgaagaa
     7081 atagtcgaaa agtgccattt agaggtgtaa aataagaaaa tgttgtcacg tataatggaa
     7141 ctagctagat aagaaatact attaaatcaa gaaaaaagaa agaaggcaca agtatgtcaa
     7201 acacagccga cgcaatagta gtgatcgatt tacaaaaagg ggtatgtttt gattcttcgc
     7261 cgatacacga attagaaaca gtgacagcat tagtgaataa aagaatcaaa acatacgaaa
     7321 cgattgggaa accagttatt ttcgttcaac acgaagatgc tcaattagtg ccacaaacaa
     7381 aaccatggga gctattgtcg gaattatcta ttcctgagca cagttatttt gtgagaaaaa
     7441 accatgcgaa ttcattttat aagactaatt tgagtgtttt attaactgaa ttggactgtc
     7501 attcgatcga attatgtggg gcacaagcag agtattgtat agatacgact gtgaagtttg
     7561 ctcatggatt aggataccag cttatgatgt gtagaggagc aacgacaact tatgataatc
     7621 cttttatgaa agcaaaggaa acaatcgctt tttacgagaa aatttgggat aaacgatttc
     7681 tgacattttt ggagcaataa gttgtttctt cgtttaaaaa aagcagaatg gatactggtt
     7741 tatccattct gctttattac gtaaaatgag aaaataatcc tgttctattt ttatgggata
     7801 aaccatttat tttttgtata aaaaatgttt gcgtttacat ataattctcc tagaaacata
     7861 aaaaggagtg aatagcttga gaacaaaaca agtattaaag ggagcgttgg tcatcttatt
     7921 tgctgtaagt acaagcgtcc cgcaatggac agtatatgga gagagcattt cttccgaatc
     7981 ggaatctcgg gaggtagaaa ccacagaaac tactgatgct ggaaatgaaa cgaaatcgag
     8041 tacttcacaa acagaaacaa atactgaaac aacggttgag acatctgtta ctagcacaga
     8101 tcaagaaacg accgctacca cagataacca acaaacagaa gaagcaacag ctacaaaaat
     8161 gtcaaacgac gaatcctctt ctgctaaagt aagtgacaaa gagaatggga cagaaaatga
     8221 tgatatcggt aaaggtgaaa atttcaatgg taaagaatat ttgaattact tcggtaaaca
     8281 gatgacagaa atacttaatg gaccaacaga agacatcaag tcaaaattaa tagagttatt
     8341 agtcactgag cttgctgtta atgattatgg tgtagataaa gagattatca aacatctgat
     8401 tgctacttat ctattgtacg atatcgcttt tgcaaaaaac agtgtgttga tgtcgatgct
     8461 tggaggtaca gatgatggga caacgcctac agaaaacaca ggcttctatc caaatacaaa
     8521 acaacttcat caaactgtac taaacaaaca agatggcaaa atgatggatg tatatgcata
     8581 ttatgtcgat caaggttcag ataaaacagt catgatccat ggcggatttc gagggaattg
     8641 gaataacggg atcgttacag aagaatacaa tgatttttat aaggccgggt ataatttatt
     8701 atttgttgat tccagagcga ctggtaatag cggaggcgat tatgtgacct atggacaata
     8761 tgagtcagat gacgtccttt actggataaa tcaagaagtt cgagagcggc catctcaaaa
     8821 gattttactt tatggtggtt cgatgggagc agctacgatg atgtctgtat tagcgaaaga
     8881 tattcctgtc aatgtcaaag gaattattga aaattgcgga tttgcatcaa ttgatgaaca
     8941 actacgtttt acgtacagcc agacagtagc ccccgcacta ccggatgcca taaaaaatca
     9001 gttagacatc attggggatc aagagcatga agatttattt atgggccttt tgaaacaata
     9061 ctattttgat caggagatgc atttggatac aaaagctgct ctacctacga ttggaatgtc
     9121 agattcattg cctaaattga ttatccatgg aacagcagat gatgtagtac ctgtgtctaa
     9181 cgcgcaaaaa ttatatgagt tatcaggagg gtataaagat cttttattag tagaaggagc
     9241 agggcatgga aaggcacagg aagttgacca tgctgcctac acgaaacatg taacggactt
     9301 tttgaaagta gtgttccatg atcagataaa cgtaaagtat gtggatgaaa acaataaaag
     9361 tctactcaaa gaccaggacg aaatccagct ttacggagct tatggagaaa attatatgac
     9421 agagcaaaag acattcgaag gatacgaatt agcaaatgtt gaaggaccga ctgaagggat
     9481 ttttaatgag acgataccaa ctattatttt ctattacaaa aaaatcccag tagctccacc
     9541 aaaaaagcaa gatccgacag agaatgcaga caataaggga aagaatgaac ctcgagagga
     9601 tcaatcaagt gatgatacgc aaccgattca gatgacagtg aaagtacaaa aaaagacaga
     9661 tgcaaaaaaa agaatgttgc cgaaaactgg agaaaagaaa cattcgcttc tcatttattc
     9721 aggtggattt ttatctgtta gcacgattat cacatggatt tggcgaagaa agagagggta
     9781 aatgaatgaa atggttcaag cgaatagtga ttgcagtacc agttatcatc gtagttattt
     9841 tagtcactgt atttttgatt aatcaaatca caccaaagcc gattagcttg cttgttagaa
     9901 aacaatttga cacaagtgaa aaagaacaag tatatgcccg accagatgat ttccagcaaa
     9961 aaatcgaaca aatcgatata aaaaaagaaa tatcctatcc ttcaaagtac aaaaataata
    10021 atatggatat ctacacaccg aaagtggaaa agaaaaaatt accgattctt ttctggatgc
    10081 atggaggcgc atatgtagga ggggataaaa atgattgtcg tgattatttg gaatatctat
    10141 gttcagacac gcagcaaatc atcgtaaata tagattatga acgatcaccg gaagcaaaac
    10201 atcctacacc agttatccaa ttgaacgaag ccattcaagc agctaaaaaa gagataaaag
    10261 atcaagcaga ttggtcgaat atttcgattg gtggaaattc ggcaggtgca caaattagtg
    10321 gagaatattt gttagcactt caaaatgaag aaattaaaaa agctgatcat cttgatccga
    10381 ctttggagaa caagcagatt acgaagttta tttctttgtc tggtctgttg gatccttctg
    10441 cacttactca agtaagtgat aaaatctcta gctttcttta tgaaaaatgt ggatgggctt
    10501 actttgataa taaggacttt gaacaatcag agaaggtcaa aagtttagcc ttggtcagac
    10561 atgcggatcg ttggacgcaa aacacattct taacggatgg aaatacaaat acgtttacga
    10621 aacaaatgga agaagtaact tctgtatttg agaaagcggg ggtgaaagtc acaaaagtag
    10681 attatgcgga gaaaaatgct gttttgaacc atgaatatca gtttgatttc agcaataaac
    10741 aagcgcaaga aacatatcaa aagttagtaa tgtttttcaa ggagtaaagt cttatgatgc
    10801 aaaagaggtt gtaatctgtc acttagctcc aagtcacaag gagcaatttc aataaacaag
    10861 cttttcatgg tttatgaata ttgtaacttg tgactgagga gttagagatg gcaaggggca
    10921 ccttttattc gaaatttggg aatatcacac gattattgtc acgatctctt ttacgtaaat
    10981 tttataagtc agatttgttg ttttgatcgt ttaaaaaaag caacaaatcc gaagcctgcg
    11041 gaggcaagaa gggacaaaaa gccgagtggt aagcaaaaga atgctggttc cactaatatt
    11101 ccgttgttat tgatataagg ctgggaacaa agatgaatag caaataaaat caatgcacta
    11161 gcgaaaaaaa gcaaagaaat atggatagct ttcataagca gcacctcctt ttttataatt
    11221 gtatgataaa tagattaaga atctataaat gtgacaagtt tgttaggatg tgaataaaat
    11281 gatcatctag cgaacttgaa acaaagatgg ctaatgataa gcaattaatg attcagcaat
    11341 cagataggta caaagtttac catcatacgt ctagaccaat aatagaaaat atgggaatat
    11401 aatagaaaag atttaggata cccccaaaag aatttcccca aaaattcgat gtatcctaaa
    11461 cttttttatt ttacataaca atcatatcaa ctgtgaaata aaattacaaa tgataaccta
    11521 aaatgaggcc aaaagccagt gttatcaagg aaatacttaa ttcaccaaaa ttttttttaa
    11581 ttttttagaa aataagaaac aaaaataata aaaaaaagaa aagataaatt ttttactact
    11641 gtatactaac tttgttatcg catctaacaa gtaactcgaa taataccaat acaaccttct
    11701 ccaaagatgc gatgacatac gttctaaccc accgactatt gatgttctgt accacccttg
    11761 cagaaccaaa aaagccacta ccaaatattt ggtagtggct ttttttgttt tatggatttt
    11821 tcgctttttc tgttatggtg ctattctttg atttcagaaa tagtatgtcg aactttagcg
    11881 atagaacgac gtgattcttt ttcgttgtaa aacatcaaaa tcacatatag agagtccaaa
    11941 atgcttagtt gagaaatacg ggaagctaaa gcttcagatc ggaattctgt ttcttcagaa
    12001 atagaaataa aaacgacatc gccaagttta gcaagggaag aattagcttg gctagtaata
    12061 acgattgttt ttgctccagt ttttcttacg gtttcagcaa tatggattgt ttcttttgat
    12121 tgccctgtgt gagaaatcaa aacagcacag tctttttccg tcaataaaga agcttccatc
    12181 aattgaatat ggtaatccgt gctgtgagaa gttgcgatag gggaacgcaa aaatttatga
    12241 taagcgtcag ttgctacaat ctctgaaccg ccaacaccga aaaaatagac acgtttagat
    12301 tcattgataa tagtagccgc tttttttaga tccttttgtt gtaacaactt ttttgtgtca
    12361 tttaaagttg aaatactaga ctcgaacacc ttttgcgcca tagtgagctc agtatctgtt
    12421 ttctgaatat tttcgtggat agaaatagca gatagatcat tttcttggat caacatggcc
    12481 attttaaaat ctttaaatcc attataacca agtttttttg taaattgaaa aaaagtagag
    12541 tcagcaatgc ccagttcact tgctaaattg ctaataggac tatgacttac tttgcttgga
    12601 ttttctaaaa tataatcggc gatagcctgt tcttttgggc tgaagtcgtt gtacattgtt
    12661 ttgatttgta gtcttatgga gacgctcatg agaattcctc attttcataa taagattatc
    12721 attttttccg ttctcattaa tgttaaagat acaaggagag taggaaaaaa tcaaacaaaa
    12781 aatatttttg taaaatctat ttacaaaaaa atttttttgt ataatgaggg tataagaaat
    12841 ggaggcgcta tcatggaaat tggtgtaatt ggtttgggaa aaatgggatt gaatttagct
    12901 ttaaacttaa aagagcacca tcacaaagta caaggactcg acatttcaag tacagctgtt
    12961 agtcaggcaa gagaaaatga cattcctgct tatcaagaga tctctgaatt tctagctact
    13021 tttgagcaga aaaaaatcat ctggttaatg cttcctgctg gagagataac cgaaaacatg
    13081 ttaagaaaac tttttccaaa actcggacca ggagacatta ttattgaagg cggaaactct
    13141 tattataaag attcaatccg tcgagcagaa gagtttcaga aaaatgaaat cggttacttg
    13201 gattgtggga cttctggagg aatcgaagga gcacgacatc aagcctgctt aatgattgga
    13261 ggagataaag aaacttttct atccgtggaa caattattca aagatgtagc aattgaaaga
    13321 ggatatctgt atgctggtgc atccggaagc ggacacttct tgaaaatgat ccataatgga
    13381 atcgaatatg gaatgatgca agcaatcggt gaaggattcc aacttgtaca aaaaagtgag
    13441 tatgcttttg acttggaaca agtcgcacgt gtttggaatc atggttcggt gattcgcggc
    13501 tggctgatgg aaatcatgga agatcagtta aacgaacatc ccaatttaag taattatcgg
    13561 ggaatcgttt ctgcatcagg tgaagcaaaa tggacggttg aaacagcgct agaaatggct
    13621 gttccagttc cagtaattgc aactagtttg ttcatgagga atctttcaca ggaagaagat
    13681 agtttttcag caaaagtagt ttcagcacta agaaatgggt ttggcggaca tgaaatagtt
    13741 gaaaagggtg agtaaaatgc cagattatca tatagcaatc gttaattcca gcagtttcgg
    13801 aagagtcttt ccacaccact tggaaagatt aaagtcagtt ggagacgttc atttctttac
    13861 tgttgatcaa gagattccag gaaaagaact agcggagtta cttaaaggat tcaatatcat
    13921 tattgcaagt gttacgccat tttttcgacg agattttttt gaaaacaaag atgaactgct
    13981 attgatttcc cgacatggta ttggttacaa caatattgat ttaaaagcag caaaagaaca
    14041 ccagacaatc gtatctatca tacctgcttt agttgaacga aatgctgtag cagaaaataa
    14101 tataactaat ttgttgtcca tgcttcgacg cacgacacaa gcacaagcaa gagtcaagga
    14161 cgatcgatgg gaagatcggg cggagttcgt cggtaggacg ctgtttaaca aaacagttgg
    14221 tgtgattggt gcaggaaata ccggaagcgg agtaatcgaa attttgcgta acggttttcg
    14281 ctgtgatgtt ctagcttatg atccttacaa atcagattta tacatcgaaa gctttggtgc
    14341 aaaaaaagta gaacttcaag aattactgga acaatcagat atcatttgtt tatgtgcgaa
    14401 tttgacagaa gagaactatc acatgatttc tgataaagaa gtcgatcaga tgaaagaaaa
    14461 tgtttatcta tctaatagtg caagaggcgc tctagttgat gaagaagcga tgattaatgg
    14521 tctgaaaagc ggaaagatcg caggatatgc cacagatgtt ttagaagaag aaccgggacg
    14581 tcaagatcat ccgtatttag cgtttgaaca agtagtgatt acgccacata tatcggccta
    14641 cacaagggaa tgtttagaag caatgggtga aaaatgtgtc agagatgtcg aacagatcag
    14701 tcagcacctt cttcctgaac gttctgtcca gacaaagagc aactatataa aaggatgatg
    14761 aagaaaatgg tgaacggttt aaaagtgaaa acaggccctc aattttattt atatgaagaa
    14821 ggtggtattt caaaagtttc tgatctcttg aaatcatacg gcgcgaaaag agtactggtc
    14881 acccacggaa cggtatcatg ggaaaaggcg ctacctaaat tagtattttt gaatgacgaa
    14941 acgattcaat ttttttatca tcgttattct ggagaatgta gttatgctga agccagacga
    15001 atagctacaa tcatcaaaaa aatgaaatcg atttcgtagt tggcgttggc ggaggaaaac
    15061 tgaccgatct agtgggttat agtgccaatc ttgcaagaat cgatttcgga gtcatcccta
    15121 ctttagctag taattgtgct ccatggacac ctcttgctgt catgtataag gagaatggac
    15181 aagcagaagg gaaaacagaa cacttttaca gacaagctgc tttttttata acagatcccc
    15241 aattagtgat tgattcccct gttccatatt tcattgccgg gttagcagat acactagcaa
    15301 aatggtatga atctgagctc atcttagaac aagatttttt gcaagatgaa tcatttttga
    15361 agctagcaca agatactgcg aagatctgca aagaagagat tttgaatcat tctgctaaag
    15421 caatccaaga tatggcacaa agaaaactga catcggagtt caaacagctt tcagagatta
    15481 tctttgctgt tgctggttta gttggaggat taggagataa atacgcaaga aatgcggcag
    15541 cacatgctat gcatgacgct atcagtaaat atattccgga aagccatcgt tttctacatg
    15601 gagaaaaagt agcgtatggg attttctatc aactggcact agaaggtcgc tgggaaaaaa
    15661 tagacgagtt atatcctttg tatcgggaat tagaactgcc ggtttctcta gaacaaatgg
    15721 gattatttcc gaaagataaa cagataatag ataaaatggc tgaatttatt gattcgaaag
    15781 agaaagtgca tttgattcct atagaggtaa cacagaaatc actgatacag tcaatgatgg
    15841 aactggaagg ttatatcaga aagatctgaa ggaggaggac acatggaaac attaagtgta
    15901 gtacaaagtt tgttgattgc tttatgggta gccgcggtta tgtcccgctg gttaggtgga
    15961 ggagccacgt tgaccttacg tttttctcct ttgatgactg gattagtcgt cgggttgatc
    16021 atgggggaag ttgcacaagc gatgattgtt acagccgctt tgcaaatgat ttatatgggg
    16081 gttttttctc caggaggatc aatgccagct gaaccatcta ttgcagcagc tatcgcagta
    16141 ccagtcgcat tattagggga tctgtcgcca gaagcagcga ttgccgttgc ggtgccagtc
    16201 ggattgctcg gaagttactt gtatcagttt cgttttttca tcaatacttt tttaggtaaa
    16261 tatacagaca aagctgtaga agatttgaat acaaaagcta tggcccgatc aattattctt
    16321 tatccaacgg ttgcctcatt tttattattc gttccgcttg ttttcattgc tttatattta
    16381 ggggcgccat tgatcgctga tgtaatcaaa gctttagagg gaacagtcgt tatccacatt
    16441 cttgaagtag taggtggagg gttagcggca atcggtatcg cgacaactgt atacgttatc
    16501 ggtcgaaaag attatctggt ctttttcttc cttgcttatt ttatgagtgt tgttttgaaa
    16561 tctttagata ttacgatggt tacttatgcc atttttggga ttattatcgc tttgatcttt
    16621 gtccagtcgc aaaaagggaa acaagtacag cctgccacaa gcaataacaa ttcagcatct
    16681 tttgatgacg aagatgacta tgatgacgga ttttaattta ggaggaatgg gaaatggctg
    16741 aaataatgga taatcaaaca ggtgctcctg aagaaatcac gaaaaaagat gtaacaaaag
    16801 cctatctgag atggcatttt gcaaacgaaa ttcctcactc ttttgaacgt tacttggcgc
    16861 catcactact atacggaatg atgccaatct tgaaaaaact ttataaagat gaagactctt
    16921 taaaagcagc atataaacgg cagttactgt tcttcaacac tcaattaagc tggggtggcg
    16981 gtgtgattac tggcctaatg tcttctatgg aacagcaaag agcagaagaa gagtatgaag
    17041 aaaaagagat cttgatgcag gatgacttga tgtataacac gaaagcggga ttgatggggg
    17101 cactagcagg aatcggagat gcaatcgatt caggaaccgt ccaatatatt tttatcgcaa
    17161 tcgcggtacc ttgggcacaa cagggaagtc ctttaggcgc actttttcct tttatctgct
    17221 ttgggttgta tcaggtttta ctaggggtat ttttcgcaag acaagctttc actatgggcc
    17281 gtaatgctac aggcctaatg caaaacactg gtgttcagac agcgattgaa atgctatcag
    17341 ttttagggtt gtttatgatg ggggtgcttg cgggaaacta tgtaaaagta gaatcttcat
    17401 tgaaatttgc gatatccgga cgtgagtttg ttatccagga aattcttgat caaatcgtgc
    17461 cagggatatt acctttagct gtcgtaatgg gtgtgtactt gttctatacg aaaaaaggat
    17521 tgaaagtcac acaagcatta ttatggctga ctggaatatt aattgtgcta gcaggaatcg
    17581 gtattttata aaaagaaggg aacgtgaata agatgggtgt agtcaattta gcgcgtgtgg
    17641 atgagcgttt gatccatggg caagtaatgg taacgttatc tcaaaaaagt ggagtgaatt
    17701 ccatttttgt tgtagacgaa gttgtagcaa aagataaatt tatgagagat ctttacaaaa
    17761 atgcaggaag ccgtacagga caaaaaacaa ttgtcattac accagagaaa gctaaattct
    17821 attgggatga atatcaattt aaagaataca attgtatttt gatcgcaaaa acagtccaaa
    17881 cgatttacga cttagtaaaa cacggaatcc ccatggaaga attgaatatt ggagggattg
    17941 ctcaaaaaaa tcctgataca gatcttttag tgacgaaatc tgtttattta aataaatcag
    18001 atgctgaaaa attgaaagaa atgaatgaac aatatggcgt aaagaatatt tattttcaag
    18061 ccactccatc tgcacctaaa acaggattaa aagatgtatt gaacaagttt gatttgtcat
    18121 aaacaaatgc ttagaaaaaa gataaagagg gcgaaaaaat gaagaagaac gagctgtttc
    18181 gtgattggga atttcgttat cgctatattt accgtaagcg ccgtacaaaa aagagtaaac
    18241 agcgttttct ttcagcatta gtatcagata tttacagtat gagaacagat gtgacagtca
    18301 ttgcttacga tacgctagcc taccgatcaa aaaatattta tgttggagat atcgaaaaag
    18361 cagagaaagt tatctgtact tattacgata cacccgttca tgcattagga agttatttca
    18421 tgtttgattg gaaagaccaa aggaaaaaaa caatctactc tatattattg tccttcattt
    18481 tgttgttcag tttaggttgg tggggaatga tgatatataa caaaaatcct catcacgtat
    18541 tcgatctgct atccgtataa acaggtatca ctgtactagc atttgggagc tactttttct
    18601 tattgggaaa agcagctcgc ggatggccta gcagacaaac ttttattcga aatacttcat
    18661 ccatattaac gatgctagaa atgatccgta cgattgatga tccgagtgta gcgtatgcgt
    18721 ttgttgatga aggctgttat ggagaaaagg gcttagacag cgtgagatcg ggtatgaaaa
    18781 aagaggctat tcttttttat ctagactctg ttggggcaga tacaccgcta cagttttcgg
    18841 gaaattattt ttcaaataaa gaacagtggc tgaaacaagt tgataagttg aaagaaaaaa
    18901 acgtcaatta tatttttagt gcaagaaaaa agcaagcgca gttcttctat ttgaccaaaa
    18961 cagatttacg agggaaaaca tttaattggc aaaatgccaa tcagattatt gctctttttc
    19021 gataagtaaa aagtgcaaaa caaggagtga gaagactaat gttggaaatc atgatcgtta
    19081 cacatggtaa gttgagcgaa ggactgaaag atgcggcgga agtaattgtc gggacatcga
    19141 gccatattga aacaatgtgc ttagagtctg gggaggacat ccaaacattc ggagagaagg
    19201 tcaaagaaaa attaactatg atcaacacag cagaacccat gctagtattt gttgatctaa
    19261 ttagcgcaag tccgtacaat caagcgctct tggcagtcaa tgaactgact gaagaaaaga
    19321 aagcagccac ttatgtcata gggggcgtga atctgccaat gttgttagaa gcaatcaatc
    19381 atcagatgct tcacacaccg atagaagaag cggtggaaca aattgtcaaa caaggaaaag
    19441 ataatttaac tgtttggcat gcttcagcgg aagaaaaaaa attagatgag gacgattttt
    19501 agaaggaagg ctgaaaagat gaagtgggga tttagatggt atggtgaaaa aggggattcg
    19561 atcccattaa aatatgttcg ccaaataccg ggaataaatg ggataattgg aactttgctg
    19621 actaaaatgc ctggagatgt ttgggagatt tcagaaattg aggagttgaa agcatctgtt
    19681 gagagccaag gactggaact gcttggaatt gaaagtgtag cgatccacga tgcaatcaag
    19741 gcagggacag atgaacgaga ttactatatt gataactata tacgaacaat caaaaacttg
    19801 tctcgatgtg gggttcgatt gatttgttat agcttcaaac caatttttgg atgggcgaaa
    19861 actgatttgt ttaatgaaaa caacgatgga agctattctc ttttatacga tcagaaaatc
    19921 gtcgatcaga tggagccagg agaaatgtat caattgatac acagccagtc aaagggattc
    19981 aaattgccgg gttgggaaga agaacggttg aaaaaattcg ctcagctgga aaaactgtat
    20041 gaaggaatga ccgaagaaga attatttgaa aatctgactt attttttaga aaaagtgatt
    20101 ccagtgtgcg aaaaagcaga tgtaaaaatg gctatccatc cagatgatcc gccatgggaa
    20161 ctttttggtt ttcctagagt gacgaaaaat cttggcgact tgaagaaaat tctaaatgca
    20221 gtagattctc catataatgg actaacgctg tgcacaggat ctttaggggc aaatccggaa
    20281 aacgatttag ttgaaatcat ccatgaggta ggttcgcgca tccattttgt ccacttcaga
    20341 aacgtggagt ttttaggaga aagaaagttc aaagaaacgg cacatccctc tacagaaggc
    20401 tcccttgata tgtatcatat tatgaaagcg ttggtgcaag aagggttcga cggtcctata
    20461 cgccctgatc atggtagaac cgtgtggggt gaagtggcta tgcctggata tggcctgtat
    20521 gatcgtgcaa tgggactcac ttacatgcaa ggtctatatg aggcgataag taaggaaaaa
    20581 ccactgggag agcattatga ataaaatcaa gctcttcatc tttgatatgg atggcctttt
    20641 attagaaact ggacgtttag cttatcgtgc ttatgtaaaa tcagcacaaa agtatgatta
    20701 tgaaatgcgt aaggaagtct attatttact aactggccaa acagaaatgg cgataagaga
    20761 acaaatgggc attttgtacg gagaagatgt tccatatatc aaatggcgag aagcaatcaa
    20821 tcaatataaa gaaaaaatcg tgaaagaaga taagagagta tatacgaaaa aaggagcaga
    20881 agaaatttta tcttttgcaa aagaaagagg aatccataca atcgtggctt cgtctaatac
    20941 acgggtaaaa gtagaaatgt atttaaaaat ggaaaatcta tatgccttgt ttgatcaaat
    21001 catttcaggg gatgatgtaa aaaaaggaaa gccggaacca gagatctttt tgaaagcttg
    21061 ttcaaaaatg aatattcctc caagtgaagc acttgtattt gaagactcga ttgcagggat
    21121 cgaagcagca aggcgagcgg gcattttgtc ttttctcatc aaagatcatt tgatagggtt
    21181 gcccgatcat aatgggaagc ataagttgaa agtagatgta agtttatatg aatccaataa
    21241 agcagactat gaattttatg acctgcatca agcaaaacga tttttagaaa gtaaagggtt
    21301 gtatctctaa taaagaggac tattttctag tcctctttat ttattgtcct gcacgactga
    21361 gggaacagat gaaaaagaag taatttttta ttttgaagta tctacctt
//
LOCUS       DACGLW010000046        21071 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.046, whole genome shotgun sequence.
ACCESSION   DACGLW010000046 DACGLW010000000
VERSION     DACGLW010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21071
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.046"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            <1..570
                     /locus_tag="IXT53_001735"
     CDS             <1..570
                     /locus_tag="IXT53_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ4905557.1"
                     /translation="APGVYQLVETQAPTGYELDATPIEFEIERSQTAVVELTKENRLT
                     PGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGAYQLVE
                     TQAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKHLPKTG
                     ETIISQFILSILGVLLVFISIKFRKKRNI"
     gene            complement(994..1164)
                     /locus_tag="IXT53_001736"
     CDS             complement(994..1164)
                     /locus_tag="IXT53_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ4905558.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(1358..1537)
                     /locus_tag="IXT53_001737"
     CDS             complement(1358..1537)
                     /locus_tag="IXT53_001737"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905559.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            complement(1547..1771)
                     /locus_tag="IXT53_001738"
     CDS             complement(1547..1771)
                     /locus_tag="IXT53_001738"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905560.1"
                     /translation="MNGSIKKILRSIDKNLMITEVSYETLQKKKTLIVAAVLFPAPCV
                     CRNCGSAVVDGNRKLMVVKNKKKETIVHFE"
     gene            1924..3036
                     /locus_tag="IXT53_001739"
     CDS             1924..3036
                     /locus_tag="IXT53_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ4905561.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(3114..3407)
                     /locus_tag="IXT53_001740"
     CDS             complement(3114..3407)
                     /locus_tag="IXT53_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HAQ4905562.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            3575..3889
                     /locus_tag="IXT53_001741"
     CDS             3575..3889
                     /locus_tag="IXT53_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HAQ4905563.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            3910..5889
                     /locus_tag="IXT53_001742"
     CDS             3910..5889
                     /locus_tag="IXT53_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ4905564.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            5902..6228
                     /locus_tag="IXT53_001743"
     CDS             5902..6228
                     /locus_tag="IXT53_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HAQ4905565.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            6244..6762
                     /locus_tag="IXT53_001744"
     CDS             6244..6762
                     /locus_tag="IXT53_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905566.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            6762..8114
                     /gene="celB"
                     /locus_tag="IXT53_001745"
     CDS             6762..8114
                     /gene="celB"
                     /locus_tag="IXT53_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAQ4905567.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(8198..9703)
                     /locus_tag="IXT53_001746"
     CDS             complement(8198..9703)
                     /locus_tag="IXT53_001746"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HAQ4905568.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(9795..10385)
                     /locus_tag="IXT53_001747"
     CDS             complement(9795..10385)
                     /locus_tag="IXT53_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ4905569.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            10564..12210
                     /locus_tag="IXT53_001748"
     CDS             10564..12210
                     /locus_tag="IXT53_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HAQ4905570.1"
                     /translation="MDNQRKPSLDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            12259..12978
                     /locus_tag="IXT53_001749"
     CDS             12259..12978
                     /locus_tag="IXT53_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HAQ4905571.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13457..14404
                     /locus_tag="IXT53_001750"
     CDS             13457..14404
                     /locus_tag="IXT53_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ4905572.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14416..15336
                     /locus_tag="IXT53_001751"
     CDS             14416..15336
                     /locus_tag="IXT53_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ4905573.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15377..16834
                     /locus_tag="IXT53_001752"
     CDS             15377..16834
                     /locus_tag="IXT53_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HAQ4905574.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            17179..17793
                     /locus_tag="IXT53_001753"
     CDS             17179..17793
                     /locus_tag="IXT53_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HAQ4905575.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            17786..19510
                     /locus_tag="IXT53_001754"
     CDS             17786..19510
                     /locus_tag="IXT53_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ4905576.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            19507..20958
                     /locus_tag="IXT53_001755"
     CDS             19507..20958
                     /locus_tag="IXT53_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ4905577.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
BASE COUNT         6796 a         3563 c         4244 g         6468 t
ORIGIN      
        1 gcaccaggag tgtatcagct ggtagaaacc caagcgccaa cggggtatga attggatgcg
       61 acaccaatcg agtttgagat cgaaagaagt cagacagcgg tggtagaatt gacgaaagaa
      121 aatcgactta caccaggcgg tgtcgtgttg acaaagatcg atgaccaaag cggagaagtc
      181 ctgcaaggag ctgtttttga gttacaagat gccaatggaa ccgttctgca gtcaggtcta
      241 accacaggag cagatggaaa actggcgata gatggcttag caccaggagc atatcagctg
      301 gtagaaactc aagcgccaac ggggtatgaa ttggatgcga caccagtaac tttcaagatc
      361 gaaaaagaac aagaagtggc gatcttttta acaaaagaga atagaaaagt tgctgatagc
      421 tcagatataa gaggaaacaa tccaacaagc agtggaaata agcatcttcc aaaaactgga
      481 gaaaccatca ttagccagtt tatattgtca atattgggtg tgctacttgt tttcatcagt
      541 ataaaattta gaaagaaacg aaatatatga gctaaaataa aaaattgaat aagcatagtt
      601 tttaaaatca actagtaccc atcaattgaa tggaaactac cgattttaga aggggtatac
      661 aaacaaatat tagtatcgga gtagagaagg ttgaacccaa ccagtaaata agtaaagaca
      721 attttgtgct gcgttgagaa gtgaaaaata aacctttgta gtaatactag ttaataaaaa
      781 atatacaaaa acactgcgaa aatcattctt gagtttcgca gtgttttttg cctaatgact
      841 caagtttatt gggtaatact ttgattttct ttctacaaat aatcagcgat cagttgtaat
      901 tttttctcta gttttaatgg ctctttgtca ataaggagaa aaacaagtcg ggtcttttgt
      961 ctcggaaagc ttcaaccaac tcgtgaataa aggttacgcc tccttgaaag gcgtggaaaa
     1021 accaagtaat cgatcaatca ccaatttcgc atttggtaga acatgtttgg tgagctgaaa
     1081 gtaggtggcg ttcatgtctg taactaagaa ttctatttct ttaggattag tgcagctaag
     1141 gaaatagttt gttaatcgag gaattttttg tgtaggtaaa ttttctcgat taatttttct
     1201 gtctcgccat ccgcggaaat aaaactcagt ttatcctcaa cggaaacgtg agaacgaaat
     1261 tcacaaccct caacactcaa gacagaattc ttttggaaga ctttggtaaa tagcttctca
     1321 actctttcaa tgtgcgaata acggttgtca aagataccta acaacttttc gaaataaaaa
     1381 ataacgacac tttttcagtc agtaaagaag tgattttatg tctgacatga tttgaaatcg
     1441 agtgccttgg acggataaaa taactttgag cgttccaatg gcttcgacag tttttacagg
     1501 tatagcgctg cttttttagg cgtataacca ttggcatatg attatattat tcaaagtgga
     1561 caatggtttc cttcttcttg tttttcacca ccattaattt cctatttcca tctaccacag
     1621 cagaaccaca atttctacag acacatggag cagggaagag aactgcagca acgatcaatg
     1681 tttttttctt ctgtaaggtt tcgtaagaga cctctgtaat catcaaattt ttgtctattg
     1741 atctcagaat ttttttgata gaaccattca taaagcatat cgttctctca gttgtttatt
     1801 ttgtggtgat ttaatcatac aagaaaacga tatgtttttt tataaaaaca tgaataggaa
     1861 aaatagttga caagaaaatg acaacgccat atgatgttcc atagtattta caacctgtca
     1921 tagatgactg taaaaaataa aagtaaaaag aaaggacgac aacaggtgga tttttatcag
     1981 tctctacaac tagatccgtt tattttaaaa caaaaaatac gagaagcagc ttctaaaaaa
     2041 gaaaagtgca tgtatatttg ttcattgttt ttacgatcat tattcatcgt attgtttgcc
     2101 atttgtttta tcattataat tacaacatta tttgaatcga agcataaacc atacgctgtg
     2161 gttttattct gtatgttgat gagtattcgt tttgttgact ttggttataa gatttcccat
     2221 tcgatcatag gtttagcaat cgtcatgttc tcactattag tagcaccgta tgtccagctg
     2281 atcaaatggt cagttatggg catgttgatt cattttattt tactaagcag catattaatg
     2341 gcaaccgcta gtgatcccaa aatggggaat gcttctttgt acggcttttc atatctcttt
     2401 attgtttatt cgttacctaa agattcgttg aacaagaatt tctttacaca aacaagtagc
     2461 ttattatttt tgtttttttg ttggttttca gttctccttt acagaaaaca tcgagaaaag
     2521 aacaaagaca aaagcttgtt taaagagatt tttttgaaag gcatgtattc gcaggaaaaa
     2581 atttggatgc tcatctatgc atttggcatt agtcttttga tcgttgcagg agagtatgtc
     2641 ccttttcagc gattgatgtg ggcagggttt gcattttctt ctattgtatc tagctatgga
     2701 ttgatgtcga ttggtttcaa agaacgagca gtagaccgaa tcattggctc attgattgga
     2761 tgtgttttat ttattggtat cagccaattt attcctttta actgggtcgg tatattaggt
     2821 ggtctagctt taggtgtttg ttctacctat agatataaaa cagtttttaa ttcttttggt
     2881 gctttggcaa tcactgccag tttattcggg gttccaggag cagtaacgat ccgcattttt
     2941 gagaatatcc ttggggtatg tttaggaatc atgtatattg gggtgactga aatacttatc
     3001 cgaaaaataa gagaaaaaca cggtttgaat cattagatga gatcgaataa aaaataagga
     3061 aaaagtgaag atacacatgg atgataaatt gctatcattc catgtgtatc tttttacggt
     3121 aaatcagtga aacgttcgac tttcattttt aatttatact ttttacggag ttcgtttatt
     3181 tgagccatca tttcggattt gtgatgcttt tcgattgctt cttcatcttt ccaacggtcg
     3241 atcagtaaaa gagattctgg atcttctagt ggaaagtaat agtcatattt ctcgtttcct
     3301 tcttctgcac gaatcgcttt gacaatgttt ctttcgacca tttcttctgc aaattttctg
     3361 gcttgtccat ttgtacttgt atacaaaata ttcactgtga tactcactaa atcccctcct
     3421 gaaaaaattc tatcactagt atatcacggt gccagaaatt attattcaag atatgataca
     3481 cattttttcc gcaatgctac ggaaatatat attttgaata gaaaacgctt acaagatatg
     3541 cttggtttat aaaaataaaa ggagtgattg atttatgaaa aaagcattga ttatttgtgc
     3601 ggcaggaatg tcatcctcga tgatggcctc aaagacaacg gatttcttta agcaaaaaag
     3661 agaagaggta ctcgtagatg cagtttcagc tacagaaggc gataatatga ttaagaatag
     3721 tgattttgat ttatttctga tcagcccgca aacgacgatg tttttggata aattcaaaaa
     3781 attaggagaa tctgttggaa agcctgttgt tagtatccct ttccaagcgt atgtcccaat
     3841 ccctacaggc atccagaagt tagcagaggt gatcgaagag aacatatgat ggaataggag
     3901 tggatgataa tgaaacagaa ggaaaaattg ctgttgagat atttgattga tcacaagaag
     3961 gagtatgtca ccagtcaacg tttagcttct gaattgttat tatctgatcg gacaatccgt
     4021 aattacttgc agcggatcaa agagttagta gaaaaaaacg gaggtaagat cattgctaaa
     4081 ccaggttatg gctaccaact gcatatttta caaagactga catttgactt gtttttgagt
     4141 cggcaagaaa tagtacctgg atatgctaga gaaccgcaag gattttatga gtccgaagat
     4201 cgacaaaagt atatattgaa ccaattgctt ttggaagatg ccattctttt tatggatgat
     4261 ttggcagaag agctttatat cagtcgttcc agtttgacaa aggacatgca agaaatcaag
     4321 gagcgattga ttccatattc actaaaaatc gtttctaagc atggtcaagg gacatggatc
     4381 gaaggggaag aaagaaatcg acggcatttt atcatggata ctttttttgg aaaccattat
     4441 ggaaattcat tgaaagaata tttagggcaa agccgttttt tcccagacat cagttttgaa
     4501 gagctagtca ttatcattct cgatgaaaca agagaagcta agttaaaagt ttcggatttt
     4561 atcatacaga atctgacttt acacttggct ttggggatca aacggctccg agaagggttt
     4621 gaaatcaagg aattaggcat cgaacaagaa gtttctaagc gagtagagta tcaagtggcg
     4681 cagcggatcg ttcaacgtat tgaggtaatc gcaggtgtat cgtttcccga agaagaaatt
     4741 gcttatttga cattacatct catggctaag tcgaatcaca aagaaaatca aaatgatcag
     4801 gaactatcaa aggaattagc gaaagttctg aaaagattgt ctcaggaatt tcatttttca
     4861 ttagtagagg atctccagct tttcaatgga ctactcgatc atttgtcacc gatgatgatt
     4921 cgattgagcc gaggtattcg cattgagaac ccattgactg aggagataaa gaaagaaaat
     4981 cccaaagtct ttgacgcagt aaaaaggcat ttcagcaaca tgccggcttt gaaaaactat
     5041 accatcaatg aagatgaatg ggcctatttg gctttgcatc tcatggcggc attggaaaaa
     5101 gaaagagctg cgcataaact acatgcattg attatctgtg caacgggtta cggcagtgcc
     5161 caactattaa agaatcgcgt agtcagtgaa ttcggtaaaa atattacagt ggtaagtgta
     5221 aaaggctatt atgagatcaa tgaaacaact ttaaaaggaa tcgacctgat tatttcttct
     5281 attgatcttt cgacaatgtt tttcaaaatt ccagttttac atgtcagtat ttttttgaat
     5341 gaagatgatg ttcgtaaaat acgaaaggta gttggagaat ctatacccta tcattcctca
     5401 gatagaccga taccagcatt ttctctacag caaaaaaagc agatttacac cgaacagttg
     5461 tctgaaaaat ttttcaagac ttatacgtac gaaccgaaaa aggaagaaat tcttcatgat
     5521 ttgctgcaaa agctgagcat aaatgaagaa gaaggatata taagagaaat gcgaaagcag
     5581 atccagcaac gtgaaaatat gggacaaatc atattcagtg atacagtggc agtcccacat
     5641 cccgctgttc cagttggaat cgaaacaaag gtagccgttg cattgattcc ttctgggatg
     5701 cagtgggatc aatatcaaga aattcatttt gtttttttag tttcgccttc ttatgtcgaa
     5761 aatgaaggaa ttacggtcgt gactaaagca atcgttaggt tagttgacca gctaaaagta
     5821 cagcaggaaa tcttagctga acctattttt gagaatttca gcagtaaatt tatcaaactg
     5881 atcgaataag gaggaaaaca gatggctgaa aaaataaaca gtgaaacact acaagtgact
     5941 tcttttgata tcattttcca tagcggaaat gcacggacac tgatacatga agcatttcgc
     6001 tcgatgcgta aagagatgtt tgaagaagta gaagagaagt tgcgacaagc aaatgaagag
     6061 attcttcatg cgcatcagtc acaaactcaa cttctgaaag aatatgccag cgggacaaaa
     6121 atagaaatgg aaattatcat ggtccatgca caagatcatc tgatgacgac gatgacatta
     6181 ttggaagttg cacaggaaat gaattatttg tatcaaaaaa ataaatagaa aggaaggatc
     6241 attgtgcagg atacagtaaa aaggaaaatc gaattagaaa aaaagcaact tagtttgaaa
     6301 aacatgtatt tcaatcgtta tctaatcgta cgttacctga ctgccttttt ctttttcatg
     6361 aacatgcaat ggatgattct gcttctatca gctaaaagtt taggaagtag tttaccgatg
     6421 attctgattt tagctatttt gcctgcagta ggagaacaag taaagcttta tcgaaaacac
     6481 caaacgaacg ttccatggac aaagtggtat tttttgttcc agagtgtctt taatttatta
     6541 gtgattccag tgttgtttac ctcaggattt acattcttgt atccttttat ggcaaataac
     6601 aatggaggac agttatttgt tttcattctt gttgtaagcg gtatttttgc cagtgcattt
     6661 atacagtatc gtttaaagaa aatttcactc aaccaagacc aacagtatat ccgtattaaa
     6721 caatatgaaa aagcgttata tttaggaaag gagaataact aatgtttgat tttttgcaga
     6781 aatacctgat gggacctatg gggaaaattg cgcaacttaa aatcgttcgg gctgtcatgg
     6841 cagcggggat ggcttctatt cctttcacga ttgtaggctc gatgtttttg gttttaaata
     6901 ttttgccatt accgttccca tttttagaag gattttttaa tgcaactttt ttcaaagtta
     6961 gtgacttgta catgatcgtc aatacaatga cgatggggat tttatctatt tactttgcca
     7021 ttgtgtttgc ttatgaatta acgtcaattg aacgagatga acaaaaatta aatgttaatc
     7081 cgctcacggg ggcattacta tctgtttttg cattctttat gtgtatccct gaattgatca
     7141 tgactgatgg taaaatcagc ttgattgctt ctatcaatga gacagaaact gtagtaaatg
     7201 gtattaggat gggctctttt gtcgaacgtt taggaacttc aggaatcttt acagctatta
     7261 ttatgtcgat tattgcggtc gaattgtatt gtatgtgtgt caagagaaat tgggtcataa
     7321 aaatgccaga tgttgttccg ccaggagttt ctcgttcttt cactgctctg atcccaacat
     7381 ttgtcattgc gtttgtggtg atgatcatca atggattatt agttgcacta ggaacggata
     7441 tcttcaaaat gattgctatc ccatttgggt ttgttacgca gttgacgaat acatggcttg
     7501 gtgtcatggt gatttatttc ttgatccatg cattgtggat cgtagggatc catggagcga
     7561 atatcatcac ttctttcttg accccgatcg tattagctaa tatggcagct aacgcacaag
     7621 gcgcaaatta tccgttggca ggagaattca acaattctta tgtaaccgtt ggtggatcag
     7681 gtgcgacttt aggattgatc atctttattg cattcctcgc gaaatctgat cagctgaaag
     7741 tgttaggaaa agcctcactt gtaccaggat tattcaatat caatgaacca atcattttcg
     7801 ggatgccaat cgtctacaat ccctatttag cggttccgtt tttcttagct ccgatggctt
     7861 ctgctacact cgcttatttt gccattcggc tggaaatcgt caaaccgatg cttgcacaga
     7921 tgccgtggcc ttcaccagtc gggatcggag catttgttgg tagtggtggt gattggaaag
     7981 ctgctgtgtt agcagtcatt tgtgcaatca tcgcattcat tatctggttc ccatttatca
     8041 aattctatga taataaatta ttaaatgaag aaagagaaaa ggcagcagaa ttagctagtg
     8101 aaaagttaaa atgatttcta agaaaaaagg aactgtgaca aaaaatcaca gtcccttttt
     8161 ttcttagaaa tgggaaacca ctagtatata aataccatta ttcttcaatc aaccgcttca
     8221 cgattgcata atccccttca taaggttcat ctacaccatt gattttttga tagggatcca
     8281 cgccttttcc cgcaataacg actgaatctt gcggagcgct ttctgctaaa gcgcgtcgaa
     8341 tcgcttcagg acgatccatt tcataatgaa cgatcaaatc tggattagta attgcttcat
     8401 tgatttcttc cgcgattttt ttcggatctt caaatgctgg gtcatctgcc gtcaaaaagg
     8461 cgatgtcagc tgtttctgat agcacttttc caaaatcatg tcgacgggag attgctttgt
     8521 ttcctgggct gcctaataca acaattacac ggccatctgg atgttcattt ttggcaaatt
     8581 tcagcaatgt tttcaagctt agataattat gtgcatagtc cacatagaca tgcgctccat
     8641 tggattggat caattgatcc attcggcctg gtacacgagc ttcttttaat ccgtcttgtg
     8701 catcttgact ggaggcacct gttagtgctg cggcaatgat cgcactggct gcattttctt
     8761 ggttgaatcc tccagccagc aaaatatcat aagcagtatc gcagatagct aagcgatctt
     8821 catgagaaga taaagtaaaa ccgtgagtac ctttatctga acggatcact tgataattgc
     8881 tttcttccga acgaccataa gtgatcgttg gaatctggaa tagatcggct gtttccctaa
     8941 gtaaatcaaa atagtcactt tcatgattta aaacaactgt ttttgaattc aagatcaatt
     9001 gtcgcttgca atagaaatag tcatcaaatg tcggatgttc gatcggactg atatggtcag
     9061 gcgagatatt caagaagatt ccgacatcta aagtcaagcc gtatacgcgt tgtgttttgt
     9121 aagcttgtga agaaacttcc atcactagat gagtcatacc attttcaact gcttctgaca
     9181 tcatacgata taaatctaat gactcaggcg ttgtcaaatg ggatttgaaa tatgttttgc
     9241 catctaacgt tgtattcata gtagaaagca atgctgtttt tttatttgtt gtgtgatcca
     9301 agattgcttt tgtaaagtac gcagcagttg ttttaccttt tgttccagtg aatccgatca
     9361 ctttcaattt attttgcgga tagtcgtaaa aagccatagc aagaagcgcc attgctttac
     9421 ggatatccgt tacgatgatt cctactgctg aattttcgta tggttgttct gatacataat
     9481 attgaatacc ctgttcgatt gctgaatcca aatattcttc tttgaaattc attcctttac
     9541 agaaaaacaa agtagaagca tcagcttttc ttgaatcata acttaactga agaaattcag
     9601 ctttttctgg tacatcaaga tgccagcctc ttgttgaaac aaattctttt aatagatttt
     9661 cttttaatag taaatgacgg atgtcttcta aagagaagct cattgattga tccctcgctt
     9721 tattcaattt cttggtttat tatagcacac tctttttaat atatcgtttg tttccgctat
     9781 gacaaaatcc agctttattc aaattttctt gacacagggg acaagcggat gagtattttc
     9841 ataaacaatg aactgactgt aatagatagg agaacgaata gtgtctccaa caatccaaaa
     9901 acataataat tcaaaaagac gataagacta ttggtaagta aaagtccttg cggcattgga
     9961 agccgaaaaa atttgtggaa aataatcggc ggtatcgtta taccgccatt tgaggccccg
    10021 actgtgtaca atgtgtaaac cccaagactg aacaaaagac ttcctgaaat cagtgaaaag
    10081 aaatgtgagg agatcatttc cacttttgga acaatctcta aaacaacagg aaaaagcaga
    10141 cttcccgcta cagtcttaaa aaatactgat tttcctaata ggacagcggc tagaagcagc
    10201 atcataagat tgatgctcca aacagtaact gagcgttgga tagaaaaggc cgtttcaact
    10261 aaatgtccga tggcgcccac tccggcggaa gctatatcgt gagggagcaa gaaccaattc
    10321 aatccaactg tgataaagaa aagacctaaa aatataacgg gtagtttttt tatttcttgt
    10381 gtcattgata tctcctataa atgttagcca taaaaggctg tatatttctt tactttacca
    10441 aaaaaacgaa accgaattca aaaaaacaat tgttcttata aaagttatat aaaatcgatt
    10501 gccagtttct gttttaccaa ttaaaatgat agtaatgttt tgtagaaagt tggaatctga
    10561 ccgatggata atcaaagaaa accatcatta gatcaattaa caccgcaaga aaaaatggca
    10621 cgaggctctg cttggatgac cgccagtaat atcatttcaa gattgctagg cgctgtctat
    10681 atcattccct ggtatgcttg gatgggcgaa aatgctaagg ctgccaatgg attattcaat
    10741 atgggataca atatatatgc attgtttcta ctgatctcta cagcaggaat acctgctgcg
    10801 attgcaaaac aaactgcacg ctataattca ttgaatgaat atggcacttc acgacgatta
    10861 tttatccgtg cattacagat gatgggcggg ttaggcctgt tgtttgcttt gttcatgtac
    10921 atcgcctctc cttggctagc tcatgcttct ggtggcggag aagaactgat tccaacgatg
    10981 cgttctttga gtatcgctgt attggttttt ccttgtatga gtgtgatacg agggtatttg
    11041 caaggcaatc aagaaatgat gccttatgcg ctgtcgcaga ttgtagaaca ggtggcacgt
    11101 attttttata tgctattggc tacattcatc atcatgaaag tcatggatgg tgactatgtg
    11161 acggcggtca cgcaatcaac tttcgcggca tttatcggta tgctagcaag tattcttgtc
    11221 cttttgtatt ttttgaagaa acatcaagcg tatacatcgg cgtttattca ctacagcgaa
    11281 aacaaaataa atattgcaac gaaggaattg ttgttagata ccgttaaaga agcgattccg
    11341 tttattattg tgggttcagg ggtaacgatc ttcaaattag tcgatcagtt cacttttatg
    11401 aagatcatgt ctgcatccac cgagtattcc aatgcacagt tactggatct tttttcgatt
    11461 tttagtgcca atccagataa gttgacgatg gtagttattg cattggcaac atcgatcgcg
    11521 tctactgggt taccgttgat tacagaagct gttacgttga aagatcgagt aggtctggca
    11581 aaattgacaa gcggaaattt acagttgttt tcatttttta tgtttccggc gacatttggt
    11641 gttatgttac ttgcctatcc gctaaataca ttattttata caccggattc tctaggaagt
    11701 aatgtactga tccaagcaag ttttgtagga ctcttcctag gactatatat gctagtttcc
    11761 aacatgctgc aaggaatgtt tgaaaacaag gcggcaatta attatttggt tattgggttt
    11821 ctagtgaaac tccttcttca atacccagcc attcgtttat ttgaagtata cggcccttta
    11881 ttggcgacaa tgatcggatt tgctgtgtct tgtaccttga tcttaaagag aatccatgaa
    11941 gtagcccgct ttaaaccaag ttttgtctgg cgcaggacat tgctgatctt tattcttacc
    12001 ttgattatgc ttttggcttc gtggatcaca aagatgattt ttggtgcttt tttaaacgaa
    12061 gatagcaaat tccagtctct attgttgatt cttgtagtag caggtatagg ggctttagtg
    12121 tatggttatt tagctttgaa aatccgcttg gcagaaagac tactaggacc aggaatagcg
    12181 cgtttacgac ataaattaaa aataaaataa gtgaatcaga agagctagat tgatgctctt
    12241 ttgaaatggg ggaaacgcat gcgtttagat aagtttttag cagatatggg acttggcagc
    12301 cgcaaagaag tcaaaggatt aatcaaaaaa ggaacgatcg aagtcaatgg acaagtggtg
    12361 aaaagcgata aagcgcaagt aaatgaattc gaagatgcgg ttacttattt aggagaaatc
    12421 attacgtatc aaaaagattt ttactacatt ttgcataaac ctgcaggagt catttctgct
    12481 actcaggata atcatgatca aaccgtaatc gatttattgg aagaccagga ttatcgggaa
    12541 gatctttttc cagtcggccg attagataaa gacacagagg gcctcttgat tttaacgaat
    12601 gacggggtgt ttgcccatca gttactatca cctaaaaaac atgtggagaa agaatatcta
    12661 gctgaggtta gcggtataat gacagaagaa gatatccgtt tatttgcaga aggactgatt
    12721 attgatggcg atgagcagac attgccggca aaactccaaa ttgatcaagt agatcaagag
    12781 acagaaactt caattatccg tctgattttg catgaaggaa agtttcacca agttaagcga
    12841 atggtcaaag cagtaggaaa agaagtctta tatttaaaaa gaatccgtat gggaaaattt
    12901 tggctgccta aagatttgaa taaagggcaa tatcgaccca tgaccaaaga agagctagaa
    12961 caagtgagag aaaaataatt gaatggatag ctaaaagaat aagcagagta gttgtagcgc
    13021 catctgaaaa gcagacttgt ccgtctgact ttcggatggc ttttttgtga aatttcggct
    13081 tatttccgta gaagctactt ctggaacacc gtttgttcgg aataagggac tgtgacaaga
    13141 cttttgttac agtccccatt gttccttgtg actcagagtt agatagtaag gacacgatct
    13201 ccctaatact caggccaaaa cgctgaattc acaacctctg ataaacggtg ttcaatcagc
    13261 tgaccctcgg tattagctca aaaaacggca aaatatgagg aactattttc ccgttttttt
    13321 ctctaatact caggccaaaa cgctgatttc acaacctctc ctatactttg tttcattttc
    13381 cctatacttt gtttatttat tctcattatt gaaagcgcta taattacgtt gcgaatgaat
    13441 cgaaaggggt ttttagatga aaaaacgaaa ggggttcaat cattttttta tgaatctatg
    13501 gcgttataaa gcacttgtcc tgatggctgt accgggtatg ctttggatga tttttttctt
    13561 ttacatacct gtaatggcga atgtcgtagc attcaaaaat tttcatattt cagcagatgg
    13621 gttcttggct agcttgaggg aaagtccttg ggtgggttgg gaaaatttca aatttttatt
    13681 ttcttcggat caagcatttc tcatcactaa aaatacaatt ttatataatg tagcatttat
    13741 ccttttgaat ctgctgatct cagtcgtatt tgcaatcatt atgagcgagt tgagaaataa
    13801 acgactcgtt aaagtttatc aaacgatgtc cttactacct tattttctat cttgggtcat
    13861 tatcagttac tttgtttacg cttttttaag tcctgacaaa ggaatattca atcagtggat
    13921 cactggacat ggaggagaag ctgtcaattg gtataatgaa ccgaaatatt ggccgtttat
    13981 cttggtgttc attggtacat ggaaagggat tggttataac agcattattt actttgcatc
    14041 tgttatggga atcgatccga cttattatga agctgctatg gttgatggag ctagcaaatg
    14101 gcagcagatc aaacatgtga cgataccaca acttattcca ctaatgacga ttttaacgat
    14161 tttagccgta ggaaacattt tccgagctga ttttggcctg ttttataaca ttcctaggaa
    14221 ttccggtgca ttatatgatg tgactcaagt tttggatacg tatatctaca atggcctgac
    14281 atccacagga gattttggta tgacggctgc tgcaggactt taccaatcag ttgtcggctt
    14341 tgtcttgttg atgatcacaa atacgatcgc ccgacgtttt gatagcgatt ctgcattatt
    14401 ctaaggagga aaaaaatgaa aaaaaagaaa gtggagagag tccaagtccg ctctttcaat
    14461 aagacggcta attttttctc taatataatg attgcactct ttgctatttc ctgtattttg
    14521 ccatttatat ttgttatcgc catctctttt accgatgagt ccatattgtc gactcacggg
    14581 tacagttttt ggccagaaaa gttcagtata tttggttata cgtacttgtt cgaccagatg
    14641 caggataaaa ttttccaagc gcttttcgtc actgtacttg ttactgtttt aggtacactg
    14701 atcaatagca cggctacttc tttatatgct tatgcgattt cacggtcaaa ctttccattc
    14761 cgtcgttttt ttaccgtttt ttgtttgatt acgatgttgt tctcaccagg gatggtggcc
    14821 aattatcttg ttatgacaaa tcttcttcag ttaaaagata cgatttgggc attgatcttg
    14881 ccaatggctg tcagtccctt caatattatt gtgatgcgca cattctttaa acgttcggtc
    14941 tctgattcga ttattgaatc ggcacggatc gatggagcta gcgaactgcg aatatttgtt
    15001 cagatcgttt taccgttagc tgttccaggt attgcgacaa tcagtttatt tgccgcattg
    15061 ggttactgga atgactggtt caatgcttta ctatacatcc aagatgataa gctagttcca
    15121 ctacagtatc ttttgatgaa aatccaaagt aatattcaat atctgacaca aaattcagga
    15181 gcaggaagcc aattgacagg cggattagcc tcagttccag gagaatctgc cagaatggcg
    15241 atcgtagtca tctcgacgtt gcctattgcg atcagctatc catttttcca gaagcatttt
    15301 gtcaaaggtc taacaatcgg cggagtaaaa gaataaagaa acaaaaatat aaatcataaa
    15361 attaggagga agaaaaatga agttatggaa gaaactagcc tttacaggtg tttcagcgtt
    15421 gttgttggga acattagcag catgcggagg ttctggttct aaagaccagg cagaagctag
    15481 caattcggac actttgcaga tgtatcaaat cggagataaa ccagataatt tcgatcaatt
    15541 gatggaagta gcaaacaaac gaatcgaaaa agagatcggt gtaaaagtca atatcaatta
    15601 cattggctgg ggcgattacg aaaagaaaat gaacgtcatc atttcttccg gggaaaatta
    15661 tgatatcgcc tttgcgaaca actatgtttc caatgcccaa aaaggagcct ttgctgattt
    15721 gactgaatta gcgcctaagt acgctgaaaa agcttataat gatttagatg aagcatatat
    15781 taaaggaaat ctggtcaatg gaaaattata tgctttccca gtcaatggaa atgtctttgc
    15841 tcaacaagta cttactttta acaaagcttt actagataaa tacgatcttt caattgatgg
    15901 aatccaatct tacgctgatg cagaaaaagt cttgaaggaa ttccatgaaa aagaaccaaa
    15961 cacggcagct tttgccattg gacaaggctt caaagtccaa gggaactttg attatccttt
    16021 agggaatacg ttaccatttg ctattgatct ggatggagat gattcgaaaa tcatcaacca
    16081 atacgataat gaaacgtata tcggactgtt acgtacaatg catagctggt acaaacaagg
    16141 actcattcca agtgacgctg caacgagcaa taaggattat ccactagaag gaaatacttg
    16201 gctaatgcgt ggagaaacac aaggaccgta tgattatggc gatacaattt tgacaaatgc
    16261 agcaaaacaa gaacttgtct caaaagcaat tactgttcca ctaaaatcta cagctcaagc
    16321 acaaatggca aattttgtcg tatcaaatac ttcaaaaaat aaagaaaaat cagtagaact
    16381 tctagggctg cttaacagtg atccagaatt attgaatggg cttgtttggg ggatcgaggg
    16441 agaagcatgg gaaaaagtcg atggttcaga tgataaaatc aaattgctag atggttatca
    16501 gccaaataca catatgtctg cttggaatac aggaaacaac aagattctat atacacaaga
    16561 gtctattacg gatgacatga ttgccaaacg tgatcaatcg attgctgatg cagaaacttc
    16621 accgatttta ggattcagct tcaacactga cagtgtcaaa acagaattaa gtaatatttc
    16681 taatgtcatg aatcaatatt tagatggatt gaatacaggg actgttgatc cagatgaaac
    16741 attaccgaaa ttgaaagacg ctttggacaa ggctggctat gataaggtat tgaaagaaat
    16801 gcaaaaacaa tatgatgagt tccgacaaga ataataatga ataaagacaa aggggttgtg
    16861 acaaaagacg tgtcacaacc tcttgttccg aataaacggt gttcaaaagc cagtttctcg
    16921 gtattaagct aaatatatgt aaaaatgtgg aaagccatat tcacaaaatt tttcttacta
    16981 ctcgaaacta agcggctatt tcacaaactc ttggtaaacg gtgttcaatc agctaaccct
    17041 cggtattcgc tcacaaaacg acaaaatatg aggaactatt tttccgtttg tttctctaat
    17101 aatcaggtca aggtgctgat tgcccaacct ttttttgtat aatgagaaag tataaactta
    17161 atgtttggag gagaagatat gttagggaag gttttggaag agttatttat ccggatctgg
    17221 gtcattataa aactaacctt gtatttctgg atatatacat ttgccggcgg gatcatcttt
    17281 ggattaggtg cggcatggaa aacagtcaat gaactatttt atttgtacgg attcgagtat
    17341 aaagaaatca caataaaaag aggctggaat atatataaaa gaaatttttt aagaggcaat
    17401 ctgctattta gtttattttt atctggtact gctttattga gttacaattt atatttatct
    17461 gttcaaatca aagggctcat ttttttagct attgatttta ttttactttt tgccttgctc
    17521 tgcctatttg ctacttatca atacagtatg attttagata gtgaatatac gatctctgtt
    17581 cctaatctgc taaagctaag ttttatttcg gttttttcca gcttttcgtc ttttctaaaa
    17641 ataattattg gtagcggtat aattttaggc gtgacttggc aattcaaagg attgatcctg
    17701 tttggtgtca ttggtctttt gacggtttgg aatggtacga tgacaacaca ttggcgtgaa
    17761 gaattagata aacagttgga aagttatgaa taagtttcgg attctatttt caaaaggaac
    17821 cttactaaat cgtctgttgc ggaaatacgc ggttatcatg atcggtgtaa cgatgactgc
    17881 taccgttatt tttagtatcc atacttggga acaaaatcaa aaacaggcag aaaacatgac
    17941 aagtgatgcc gttcaaagca caagcagaat gctgaatgat aaaacaacct tgagtaggat
    18001 catcaaaaac cagcttgtag ggaattccga aaagatcgaa aatgtgacga cctacttaac
    18061 aaaacccata gatcagtatc tgatgtatgt atacgaacag caaaacagta ccgatgaatt
    18121 agtttctttc ccaaatcaaa tcaaagatct ctacgcaaat tatgaagaac taagtgcgat
    18181 atatatcgtg ttgaatcaac taccggagta ttatgaatct tctagagaaa acaaaggtgg
    18241 ggaaataaaa aaggggacac caaaattact agacgccttt tatatcaagt tgcctatcac
    18301 acaaggagga gctgagtcag gaagtatttt tattggtttt caaaagaaag aactagatat
    18361 gattttagaa aatctaacgt cattcaatgg actctcttta tacatgattt ctggaacgac
    18421 aaatcgacta tacacattcc atgataataa aatagctaaa gaaacgtttg aaagacaaga
    18481 aatgatcatc acagagagtc tgaagaaaaa ttcacaattg ccgattgacg atctggaaaa
    18541 aaacaatttt gttcaacacc aagaactgtc tgatgattat cgtatattag ctgtactaga
    18601 taaaaaaagt gtaaaaatcg aaacgggaaa aaatcttgcc ccgctgatca ttggtatcat
    18661 tattttagac aatatactgc ttatctttct ttataaaaca ttcaaacgat attcacagca
    18721 agtatctatc gtcatgcagg ccatggatca aacagcagca gggaatttag agacgcggat
    18781 cgacataaca aacacggaat atgaactgaa ggaactttca atcggtatca acgaaatgtt
    18841 ggatagcatc aatcagtttg tagaagacat ctacaaatta gaaatcaaac agcaagacgc
    18901 acatatgcgg gcgcttcaag cacagatcaa tccacacttt ttatataata cattagaata
    18961 tatccgaatg tatgcaatca gcgaaggaag tgaagaatta gcagatgttg tgtatgcgtt
    19021 ttccgctttg cttcgaaaca atacgaatca agagaaaacg atcactttaa aagaagaact
    19081 ggacttttgc gaaaaatatg tctatttgta tcaaatgcgt tatcctaatc gagtagccta
    19141 tcattttatg atcgatcctg atttggaaaa gatcgaagta cctaaatttg tgatccagcc
    19201 attagttgaa aattatttta aacacggtat tgattttact cgattcgata atgcgctaag
    19261 tgtcaaagtc ttgcaggaag gcaaacgggt aaggatcatc atcaaagata atggaaaagg
    19321 gatgactgaa aaacggctaa aacagattga agaaaaactg tctcatccta aagtggaact
    19381 tcacggttct atcggtctgc aaaacgtgaa tgaacgttta cgtgccagct tcggcagcag
    19441 ttattatatg tcgcttgaga ataatgagac aggtggactg accgtttcta ttacattcaa
    19501 ggagggatga gcgtgtatca agtgttattg gtagatgatg aatacatgat tttgaatggg
    19561 ttaaaaaaga taattgattg gcaaagttta ggctttcaaa tagtagcaac agcagagaat
    19621 gccaaagaag gactagctgt acttgaacag cgacagattg atttagtagt aacggatgtg
    19681 acaatgccag aaataaatgg actggaattt attgaagcgg ctcaaaaaga acgtcataat
    19741 tttgaattta tgattttatc aggataccaa gaattcgatt atttgaaagg aggtatgcaa
    19801 ttaggcgccg tgaattattt gatgaaaccc gtgaataagt tcgaattggt tgaaagcctc
    19861 aagaaaatca aaacacggtt agatcagcaa aatgaacaaa aaaatcaaca agaaatctat
    19921 caagaactgt tattcagtca atggttgaat gaagaattgg atgaaataag tgaagaagaa
    19981 ataataggac aaataagtga gaagcagcat aggatagtat tgattcaact ggcaagaact
    20041 catgatactc ttatcaatag atggttgcaa gaacggcaac aaatctttta ctatcaacga
    20101 aattacggag accgtgtatt gatcacgttg ttgttggagg cagatgaagt tgatggcttc
    20161 tgttgttttg ttcaagaaaa ctttcctgat caggaatggc tgatcagtat cggggaagag
    20221 acagattctt tggacaaaat ccctgaaaca ttccaacaag cgaaagacaa tctacagttg
    20281 catcaatttt atggagataa agacgagcat gttttttacg cagtacaagc agaaacaaaa
    20341 gatcaaacca tcgatttttc aagtttcaga cgcgtgctgc aaaataaaca attggaagtc
    20401 gctcaaaaaa tgatcactga tttttttgaa cagtttcagt tagcagccat gatgccggaa
    20461 gatatacgat attccagttt tttactgttt atggagattc aacgggaatt gattgatctg
    20521 gaagatgagg aatatttaca aggaatcgaa aaaatccatc aggcaaaaac ggtgcaagaa
    20581 cttcagcagc tacttctgtc attcatcaaa aagcatcaga gacagaaaaa gtacagtgac
    20641 aatgtggaaa aagtaatcgt aatcctgcac cagcattatc aagaaccact tacactcaaa
    20701 gaagtgtcag aaaacctgca tttaaatgtc atgtatctag gacagctgtt caaaaaagaa
    20761 acgaaaaaaa gtttttcagc ttatttgaac catttacgta tggaaaaagc gaaacaactg
    20821 cttttgcaca gtaatcaaaa catcaatgag atagcaagtg agattggcta caacaacacc
    20881 acgtatttct ctaaattatt caagaaaatc gtcggcagat cccctaaaga gtaccgagaa
    20941 aacagtggag aatcataaaa gaaaaagcca atgtaatgaa ataacttata aaaaattgtc
    21001 tagaaatcta attttgggct gttcttatta cttgaggcaa ggcagctttt tcatgacctc
    21061 tcgataaacg g
//
LOCUS       DACGLW010000047        20663 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.049, whole genome shotgun sequence.
ACCESSION   DACGLW010000047 DACGLW010000000
VERSION     DACGLW010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20663
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.049"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            167..361
                     /locus_tag="IXT53_001756"
     CDS             167..361
                     /locus_tag="IXT53_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905578.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            630..929
                     /locus_tag="IXT53_001757"
     CDS             630..929
                     /locus_tag="IXT53_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905579.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(1026..1460)
                     /locus_tag="IXT53_001758"
     CDS             complement(1026..1460)
                     /locus_tag="IXT53_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ4905580.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1692..2402)
                     /locus_tag="IXT53_001759"
     CDS             complement(1692..2402)
                     /locus_tag="IXT53_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HAQ4905581.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2392..3252)
                     /locus_tag="IXT53_001760"
     CDS             complement(2392..3252)
                     /locus_tag="IXT53_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HAQ4905582.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3257..3895)
                     /locus_tag="IXT53_001761"
     CDS             complement(3257..3895)
                     /locus_tag="IXT53_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HAQ4905583.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3910..4209)
                     /locus_tag="IXT53_001762"
     CDS             complement(3910..4209)
                     /locus_tag="IXT53_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ4905584.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4252..5736)
                     /locus_tag="IXT53_001763"
     CDS             complement(4252..5736)
                     /locus_tag="IXT53_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HAQ4905585.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQVLIFMGLILGCYWAVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5756..6217)
                     /locus_tag="IXT53_001764"
     CDS             complement(5756..6217)
                     /locus_tag="IXT53_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ4905586.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6235..7302)
                     /gene="ulaG"
                     /locus_tag="IXT53_001765"
     CDS             complement(6235..7302)
                     /gene="ulaG"
                     /locus_tag="IXT53_001765"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HAQ4905587.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7520..8290
                     /locus_tag="IXT53_001766"
     CDS             7520..8290
                     /locus_tag="IXT53_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ4905588.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8563..9390)
                     /locus_tag="IXT53_001767"
     CDS             complement(8563..9390)
                     /locus_tag="IXT53_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ4905589.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9958..11193
                     /locus_tag="IXT53_001768"
     CDS             9958..11193
                     /locus_tag="IXT53_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HAQ4905590.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12192..12452)
                     /locus_tag="IXT53_001769"
     CDS             complement(12192..12452)
                     /locus_tag="IXT53_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HAQ4905591.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12479..13780)
                     /locus_tag="IXT53_001770"
     CDS             complement(12479..13780)
                     /locus_tag="IXT53_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ4905592.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(14072..14530)
                     /locus_tag="IXT53_001771"
     CDS             complement(14072..14530)
                     /locus_tag="IXT53_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAQ4905593.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(14543..15868)
                     /locus_tag="IXT53_001772"
     CDS             complement(14543..15868)
                     /locus_tag="IXT53_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005227470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HAQ4905594.1"
                     /translation="MKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDEER
                     NMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKIPL
                     KHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVRRK
                     YPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREIMP
                     EIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVVEH
                     TDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLNDDR
                     RRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAVEK
                     LLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(15885..17297)
                     /locus_tag="IXT53_001773"
     CDS             complement(15885..17297)
                     /locus_tag="IXT53_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ4905595.1"
                     /translation="MNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADDMLNIPVIGAA
                     GNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMDPEVNMGIFGG
                     IISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLGFIWPLIQIGI
                     DNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSPSKEVFRGEMT
                     RFINGDPQAGLFLSGFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMGSGAGTSFIAN
                     ITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVDYLINFRIATN
                     AILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEETELGLASKNYE
                     YMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISGIIKPTEKNYQ
                     IIIGLEVTYVMAEFNKLLEE"
     gene            complement(17314..18078)
                     /locus_tag="IXT53_001774"
     CDS             complement(17314..18078)
                     /locus_tag="IXT53_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HAQ4905596.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(18182..19057)
                     /locus_tag="IXT53_001775"
     CDS             complement(18182..19057)
                     /locus_tag="IXT53_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ4905597.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(19312..19396)
                     /locus_tag="IXT53_001776"
     tRNA            complement(19312..19396)
                     /locus_tag="IXT53_001776"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19360..19362),aa:Leu,seq:caa)
     gene            complement(19414..19485)
                     /locus_tag="IXT53_001777"
     tRNA            complement(19414..19485)
                     /locus_tag="IXT53_001777"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19451..19453),aa:Gln,seq:ttg)
     gene            complement(19495..19567)
                     /locus_tag="IXT53_001778"
     tRNA            complement(19495..19567)
                     /locus_tag="IXT53_001778"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19532..19534),aa:His,seq:gtg)
     gene            complement(19629..19713)
                     /locus_tag="IXT53_001779"
     tRNA            complement(19629..19713)
                     /locus_tag="IXT53_001779"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19677..19679),aa:Leu,seq:caa)
     gene            complement(19734..19804)
                     /locus_tag="IXT53_001780"
     tRNA            complement(19734..19804)
                     /locus_tag="IXT53_001780"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19770..19772),aa:Cys,seq:gca)
     gene            complement(19850..19921)
                     /locus_tag="IXT53_001781"
     tRNA            complement(19850..19921)
                     /locus_tag="IXT53_001781"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19887..19889),aa:Gln,seq:ttg)
     gene            complement(19933..20005)
                     /locus_tag="IXT53_001782"
     tRNA            complement(19933..20005)
                     /locus_tag="IXT53_001782"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19970..19972),aa:His,seq:gtg)
     gene            complement(20008..20081)
                     /locus_tag="IXT53_001783"
     tRNA            complement(20008..20081)
                     /locus_tag="IXT53_001783"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20047..20049),aa:Trp,seq:cca)
     gene            complement(20088..20168)
                     /locus_tag="IXT53_001784"
     tRNA            complement(20088..20168)
                     /locus_tag="IXT53_001784"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20132..20134),aa:Tyr,seq:gta)
     gene            complement(20170..20245)
                     /locus_tag="IXT53_001785"
     tRNA            complement(20170..20245)
                     /locus_tag="IXT53_001785"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20210..20212),aa:Phe,seq:gaa)
     gene            complement(20281..20353)
                     /locus_tag="IXT53_001786"
     tRNA            complement(20281..20353)
                     /locus_tag="IXT53_001786"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20318..20320),aa:Asp,seq:gtc)
     gene            complement(20363..20435)
                     /locus_tag="IXT53_001787"
     tRNA            complement(20363..20435)
                     /locus_tag="IXT53_001787"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20400..20402),aa:Val,seq:tac)
     gene            complement(20451..20522)
                     /locus_tag="IXT53_001788"
     tRNA            complement(20451..20522)
                     /locus_tag="IXT53_001788"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20487..20489),aa:Glu,seq:ttc)
     gene            complement(20537..20626)
                     /locus_tag="IXT53_001789"
     tRNA            complement(20537..20626)
                     /locus_tag="IXT53_001789"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20590..20592),aa:Ser,seq:gga)
BASE COUNT         6531 a         4321 c         3274 g         6537 t
ORIGIN      
        1 aagaacaata gccttttatt atatatatta ttcaaattag ttatatcgag tgaaaaaaac
       61 tttcaaattg attcttttta tttatcataa agatatagat acataatcat tttcacttac
      121 atatatttga atattaacta aaaacagcaa gaaagaagag gatcaaatgt attatgtaga
      181 agtaaaaaca aaaggcgtca aaaacaaaca atatgtgaaa ggtatcagta acgaatatcc
      241 tttacttggt tcatggaaag aagctgctcc tttttccaaa ccatgtgcca taaaaataaa
      301 gaacgagcta gaaaaagaac tgacttgtgg taaagctgtt gtagatatca tcgaaaagta
      361 aatccaatgt atagaaagga agatcacaca tgaaaagcga cgtacaaatt gctaagatta
      421 tagaattaga agaagaagac gcagtgttga caattgaaaa aaaataaatg tttgtatacc
      481 atatgattat ttcaatatgt atcctggaat tggagatctc gtagagctat atcaagatga
      541 atcaaatata cttgtcattc atcatgaaat atagagactt ataaaattgt ttatttttat
      601 aatgtatcag tatagaaagg aaataaaaca tggattatac tgttgaattt gacagtgaac
      661 tacttaagca atttccacaa gaagaacatt tcatgtataa agaagcaatt tcccacaaag
      721 tgacagagta tttggagcat aatttttttc gtataaagcc agtgcgtact cgttcggcaa
      781 aaggaatata cgagatgaaa gtcaaaatta ataaaaaaaa ttatcgcata gcatttgctt
      841 taaaaaaaaa tcatgtgttc attttttata ttagttcgaa tttgcaaaaa attgtatttg
      901 ataaagaggt gagtaaaaaa ataaaatagc tgagcattgg aggatagatt gagtgatact
      961 aagtctatcc tcttgttttt ctaaagaact aagctagaat tttttctttt gtacgttatt
     1021 atatgttatt tgtcattacc aaaaagcacc ttttttcttc tagtatctat gacataagaa
     1081 ataccaccca tttcctttac ttgaacaaat cctactgatt cataaaattt ttttgttttc
     1141 tcagtatcat ctgtcaataa ttcgatttgg actggtccat aactagtgat cttctccaaa
     1201 gcttcattca aaagcacttt gcctattcct tggcgctgat acctcggatg aatgaggata
     1261 tcttgaataa atgcgatcgt cgcaaaatca gtgatccaac gaacgattcc gataatcttc
     1321 ccttccccat tacgctttat cagagattca gattgttcaa atgctctagc taaccttgca
     1381 gtgtcttttg tgtaattcgt ccaaccaacg ctcttatata attcagaaat ctcatcgatc
     1441 ataagggaat caatattcat ttttttcttc ctttcttata caattctccc tctaataata
     1501 cagttctttg actatttaat ctatcacata taactctcct gtaaagattc tctcgattcc
     1561 ttaaaattcc tctttccttt tagaatgcat actttttgat aaataaaaag gttgtgataa
     1621 attttcttca caacctctgt ttctctctat caagtgttat cttttaattt tctaccctta
     1681 ccctagcatc tctattgccc gtagtatgca tactttccat gtttgcgaaa ataatgttta
     1741 tctagtaaat attgcgggat ttcacatata ttggggttga tcagctcgtt ttctttggcc
     1801 atcagacaaa tttcatctaa gatcaaactg ttttctactg ctttcatagg tgaatccccc
     1861 catgtgaacg gtccatgtcc atagacaagt actccaggga cttcgtttgg atctaatttt
     1921 ctttccttaa acgtctcgac aatcacattc cctgtatgaa cttcgtaagc ttctcttact
     1981 tcttctttcg ttaattggcg tgtacaagga accttaccat aaaaagcatc agcatgagtc
     2041 gtgccataag ctggcaaatc gcgtcctgct tgcgcccaca tgacggagtg cgtggaatga
     2101 gtatgagtaa tagcagttat atcttcaaaa gtttgataca aaacaacatg tgtggggaga
     2161 tctgaagatg gttttaagct atcttcttct attatatttc ccgacaaatc tgttaccacc
     2221 atttgatctg cttgcatgca ttcatatctg acaccgcttg gcttaatgac gatgactcct
     2281 agctcacggt ccacttcact cacattgccc caagtcaatt tgactaatcc gtatcgcggc
     2341 aatgccagat ttgcttcaag gacacgttgc ttcattgctg caattttttc ttcacaagac
     2401 atcatagcca gcctccttca atttagaact taagtagtct tttgcctgct ggatttctct
     2461 tcgaaagtct tggctttttt cactccacat ttcaatcaga aatgttccat cataatctag
     2521 acgtttcaaa tgtcttaata atccaagaaa atcaacacaa ccgctaccaa aaggaacatc
     2581 acgaaactgg cctgaatatt cttttgttac agggtaagta tcttttaagt ggatagcagt
     2641 gatataatcg atccctttct caagttctcg agccggatta ttttccggcc atgcggacaa
     2701 attgcctagg tctggatata cttgtagaaa aggtgaatga atctgttctt tgatttctaa
     2761 aaattttgaa atggagttca taaatggatc atccatgatt tccacggaaa gaatgatgcc
     2821 atatttagaa gcttccttta cgctttcttt tattccttcg ataaagtctt ctctggaatt
     2881 cattgatttc tcttcataat aaacatcgta tccagctatt tggattactt tgacgcttag
     2941 attatgcgcc aaatggattg cttttttcat tagttgtttc gctgcttttt tcttttctgg
     3001 atcagcagaa ccaaacggaa atcgccgatg cgcacttaaa caaatcgaat ggatacgtac
     3061 accggagctg aacatctctt ctctcagctc agctatcttt tcttcagacc aatctaatcg
     3121 tgctaaccgc tcatccgtct catcaatcga catctcgata aaatcgaagt ccatttcttt
     3181 tacaagggaa aaacgttctt tccatgaaat atctttgggt aacgcttttt cataaatccc
     3241 aatttgagcc atttccttac ccccagatac gaatgatctc atctttgaag ttttgtgcgg
     3301 ccttcatcgg atcatttgct gctgtaatac cacgaccagt gataaaggta tacacatcga
     3361 tcccttcaaa taagtgtaat gtgtgtggat ttaatccacc agttacagaa acattaaagc
     3421 ctagttcaat cagttttttg atcttcgata aatctttttc accccaagtt tcaccagata
     3481 acagcgcatc acggctttga tggtagatta cctgccggat accgatgtta tgccaatcca
     3541 tggcttgttc aaaagtccaa ttgccgtaga gttctacttg caattcacct atttcttttt
     3601 gtgctgcctc catagttgga atcgttgcac aacaaatgac tgtcatccag tctgctcctg
     3661 cttttgcaac attactcgcc actgttcctc cagcgtctgc acatttcgta tcagcgacta
     3721 gttttttatc tgggaacata ctgcgaatac agcgaatcgc tttttgtcct tcctgtaagc
     3781 aaagaattgt gcccacttca ataatatcta ctacatcacc aactttcata acatctccta
     3841 atgcatgttc taagtcattg tgatctaacg caatttgcaa attcggcctg ctcatcttag
     3901 ttccttcctt tacttgtttt tttggtacca ctttgtttca gcaaattttt cactgatttc
     3961 tttgactgac atcacatttt tgataccgat aactgtaact ccttttttct ctgcctgagt
     4021 aaacatattc aaaaaattga ctggcgtaaa aactacatcg tattggccag ctgaactttt
     4081 tccctctgaa atggcacaat gatggatttt ggtaattggt acgtttagtt ctttcattgc
     4141 tttttccaca cttctcatca tcatcaagct tgttcctgat ccatttgcac acgatactaa
     4201 aattctcatc ttaatccctc cattatgagt gttgtctaat cagattttct tttatactct
     4261 ctgcttttct gcatatgtgt catagtcttc tacaatcaaa aaataaccat caggattttt
     4321 ccgatattgt aactgcggaa tagccaataa accaatgaca actacggcaa ttccagcata
     4381 acctaggact ttcatgatta ctgtaaacaa tggccaagct gttgcccagt cgaacatccc
     4441 gatataacca ccgtatgttg acatcccaat ccatgtagcg aataatgctg aaccgcctac
     4501 ttgtatgatt cctgaaagaa acgggaacag gcaagcggct ttgaatccac ctcggttatt
     4561 ggcataaact gctataacgg cattatcaaa gaacaacgga atgaaaccgg ctatgacgat
     4621 cgttggtgat ttcaataaaa tcagtaaacc aatcatcaaa aactgtccca aagcaccaaa
     4681 caagaaccca atcgttaccg catttggcga accaaagcca aaagtagcag ctacgtcaat
     4741 tcctggaaca gctcctggta aaatcgtgtt tgagattcct tggaaagatt gagttagttc
     4801 atcgacgaat gtccgaacac ctaattgcaa gatcgccaga tataccgcaa aatttaaaga
     4861 agtcgtcata atgtagaata agaaactctg cccttcttgc atgaattgcg cttgaatcaa
     4921 ataatctttg cctagtacaa tcagaatgat cccaaagaaa aatagcatga gtaacgaagt
     4981 agcaaccata ttctcattga aaatcgacaa gaatcctggt aatttgatat cttcaatttt
     5041 tttgtcttgc ttgattgatc tatttttctt ttcatcccgc tttttcatcc actcagctaa
     5101 ttttgcaaat ataaagattc cgaacatttg ctgatgtgcc actgcaaatc cggcaccttc
     5161 tgtcaattcc tgcgtgattc ctactgttaa gttagaagct actgcccaat aacaacctaa
     5221 aatcagtccc ataaaaatca atacttgtat cctacctaga tcaggaaagc aaaacagtaa
     5281 aatccaaaaa gcggtagctg cttgttgaac ctgaacgttt ccggtagtga aaacggaacg
     5341 cagtttcgta tatttctgaa aacggactaa taagatattt aaaacaaatg caatcagtaa
     5401 taaaatcatc acatctccaa aggtacggcc aaatgtctcc attaatccag catccacagc
     5461 attttgtcca aaataaggat ctgtaaccat ggcatccaaa ttgaaccgtt cctttagtcc
     5521 aaccaagatt ggccgaaaat tattgaccaa accaccagag ccaacagtca aaataaagta
     5581 accgaccgtt gcctttaaga atccagcgat gcattcatga agcgggcgtt tcagcaaaat
     5641 atagcctaat aacacgataa aaccaatcag gtatgctggc tgcgtcaaga tatttgttgc
     5701 aaaatacgtc caaatgctta acaaaaattc ccccatcttt tttctcctct atcaattaat
     5761 tatattctgc catcacaact tggtaatcat cgatggagtt cgtagagatg agacgctcaa
     5821 ccaatccatc tgtcattaat aattcggata atttttggat attttctaga tgttcttctg
     5881 gatcttttgc tgcgagtgta aaaaacaaac tagccgtttt ctcctcattc tccccttcac
     5941 tttcaaaacg gattggctgt ttcaacttgg taaatgaaat agctgttcca aaaacaccat
     6001 cacttttttc tgaagaatgc ggcattgcta cttgaggtgc gatcacaata taaggaccgt
     6061 atttttctac acaatggatg atctcctcca catacgtttc atcaataatt tccttatcga
     6121 tcaatacaag acagctttcg gcgatagctt cacgccaatc cgtgatttct ttatctgggt
     6181 aatgaatcaa ttgatggtca aaaaagtatt tcagcaagaa actccccttc tttcttataa
     6241 aaacgacgga aacggtgtat cattttcaat cgagaagaca tcttcaaatc ctcgataaaa
     6301 attaaactca agatcatctt tgtttgtcgg atagatgaat ttcccgccta cttgccagat
     6361 ataaggttta aatccatact tcaggcgatc ctttttgaat ttccaaattt cagtgatctc
     6421 ttttggatca gccataaaat tagaccaaat atcgtagtgg acaggaatca ctacttttgc
     6481 attcaatgac tccgccatgc gcagcatatc aacagaagta actttgtctg ttatccctcg
     6541 tgggttttca ccataggccc ctaaacagac atcgattttg tgttcattac catgtttggc
     6601 aaataaattg gagtaatgtg aatctcctgc atgataaaga ttccctcctg atgtttcgaa
     6661 taaatagttt actgcaattt gatccatctc ctgaggcatt tttcctttta atacctcttc
     6721 tggatcttca caagtgacca acgctgtccg atcaaacgct tctaaagcta ctacattgat
     6781 gtctttgatt ttgactgcat cacctggctt tactgtaatc gttttttctt caggaattcc
     6841 ccatttttgc cacgtgcgaa ccacttcttt tggcccaatg aatgttgctt caggacaatt
     6901 ttgatgaact gctgcggctg tatggatatc tagatgatcg gaatggatat gtgtaacgat
     6961 taaagcgtct acatttttta tcgcaaaagg atcgattacg aaaggctgag tacgtaaatt
     7021 aggctgcata ttttgaacgc cactcatacg catcatctga tggccttttt tcatcatgcc
     7081 attcccatga ctacgttttc ccgtcccaca ccatagatca cataaaatat tcgttccttc
     7141 atgacttttc agccaaattc ccgtacagcc cagccaccac atagcaaaag ttccaggatc
     7201 cactacttct gtttcgattt cttcattcaa gtatgtgccc cactcaggaa atgtttctac
     7261 taaccacttt tcttttgtta cttcattgac agttttactc atttgcttgc tccccccata
     7321 tcgttgttcc aacttacaag taaatattaa tgtgtatcag catccatgaa aaccctttta
     7381 tatgaccgtt tgttttttat atgattttct ttcagtaatc aatgattgtt tcagtataat
     7441 aaagacagat atgaatgttt aaatgaacaa ctagtcaaat aaatccatgg atcaagatgt
     7501 ccctcaggag gcaaaagtca tgaaaaactc acttgtcaat attgaaaaac gtcaaaaaga
     7561 aattttggaa ctgcttcagc agactgaaca actatcaact ttcgaattag ctgaatccct
     7621 tgatgtatct attagtacga tacgaagaga tttaaattta ttagaagaaa aaaacgagat
     7681 catacgcaat tacggttatt gttcttataa ttacaaaaac cagactgatt ttgatcaatc
     7741 tggtcctgaa aggattaaac aagcgattgc cagagaagct agtaaataca ttaacgatta
     7801 tgatacgctg tttatcaatt ctagctcgac tgcgttgaaa acactggatt ttcttcaatc
     7861 taaccatttg acagtcatta caaacaacgt gaaaatcaat tactctccgc acaaagataa
     7921 ttgtagttat attttaactg gtggagagat gcgctttcca aaagaagcac tagtgggcga
     7981 catcgcatta aatacgattt cttcagtcaa tgcagacatt tgtatcattg gctgtagcgg
     8041 agtcgatgta gtacatgggg tcacaacaaa gatattaaat gaatcaacga tcaacgaaat
     8101 gatggtcaac aagacaatca aatgtaagat tttagtcgct gatcatcgaa aaatcggact
     8161 gacatcaaag ttcaagattg ctgatatttc agtctttgat tatttgatca ctgataaatt
     8221 ttgttctgaa aaagtattag aggagatcag aaatacgggg ataaaaacca ttcaagtaga
     8281 ttcattttaa gaaccaggac gtaaatatgt aaataggatt atcaaaaaaa tgaataacaa
     8341 atttatcgat aaatgaaaaa cacgctacgc tcaaattgat aaaggaatcc caagatgtat
     8401 ccatccaatc gataccatca taatctattg cttcttttat aaccgttata tgttgcctaa
     8461 ataggaatca aacaatatcg aaaaaaggag gtatgacaaa agtcacacct ccttttttcg
     8521 aataaacggt attcaaatca gaacactgat ttcacaacca ctttatttca ggtcgttttc
     8581 aatcaccttt accgcgtaat catttccgtt gaaacgcgct attttttttg cgtccatata
     8641 tctttctaaa taatcatctt ctcctaattg cttatggcaa gaagcaattt cataaagcaa
     8701 gtattccaat gcataagtcg atttatgttt cttattatac tcaataccct tctcacataa
     8761 ttggattgct ttatcataat cctcgatttt acggtaatag gtggcgatat cataatatac
     8821 aatgcagacc atcagattat caaaatcagt ctcactcaat attttaagtg tacgctccac
     8881 ataaactttc gcaaaatcta attctccttt tgacaagtag atcattgcca cgtttgcagc
     8941 tgcttgtaag tgatggatat ctgtttcggt cgtttgttcc aacacttgat taaaataaaa
     9001 taatgctgca tctggttttt tcgcataaag atgatcaagc agtccttgat aaaataaaat
     9061 ttgatttaaa agatcagaat tacttacttt ttcctgatcg atcgtttgta aaattttata
     9121 agattcttgg tattttgata cagaattcaa tgattgaatc tcatttattt ttttcctgat
     9181 tttgtcttct tctgtttcta ctaatatatc ggagatctcg acattcagct ttttgcaaag
     9241 cgcacttaag atattgatgc ctggcgacat attcttgttt tcgatattgc tgacagttgc
     9301 ttgttcacaa atgccttctg ctaattgtac ttgtgttaac ccctttttct ttcggtattc
     9361 ttttagtaat gctccatcta ctttaatcaa aacaatttcc ttccctttaa aaaattattt
     9421 ctcctttata tcatacacaa aaaaaagttc taaacaacca aaaatgttta gaacagaata
     9481 aaaattttaa ttttaaaata atattcaaat taaacaactg gcgtatctgc tccttgattt
     9541 ctttttgtgt tatctgaata attatcgttt acagttataa cacatagtga aaattgtcat
     9601 ggcgagaaag tctaagaata tttttaaaca taactatttc tttcttacgt gtgtataatg
     9661 acgacagcac acctatcaat taatataatt attttatttt tttatgttct catgtttatt
     9721 tttaatgaca caacttctcg tatttatctg gcacaacata taaatcgatc aaaaaattgt
     9781 tgcgtgtttt tttgaaaatt ggagtttcaa cttgattaca aaatttaaat ttagaagttg
     9841 tgaaaacagc gagagacagt ttaatattca aaatatacat aggctactcg aacgctgtgt
     9901 tttttagtat actagtaaga aacgaaggga tagactcatt ccaataagga ggatttcatg
     9961 aagacaattc tttttgctcc tgcagtattc aatttagctg agacaacacg aatgattgag
    10021 gttgccaaag ctatgaaaga ccgttattac tgtgtattct ttggcttttc taaaactttt
    10081 gctcctttga tcacagaaag tggctttgag tatcatttac tagcgccgat ccttacgaaa
    10141 aaacaagaag aacaaatcat aaaatttgac cagttgcgta cacttaaaaa cccttttacc
    10201 aaagaaatgg ttgcctctcg tatcactagt gaacagagtc tgattgctaa gtttgagcct
    10261 cgactgattg tgacaggttc gaatgtgaca atttttttat cggcccgcaa taaaaagatt
    10321 cctttagtat atgttaaacc ctatgctttg agtgaacctt tttttcaatc agagcatgcg
    10381 ctgatgcccc acgagttacg tttcttcggc cctttaagca atcccctttg gcactggaca
    10441 aaaaagcgta tagtaaatac ccgatggatc cccaatgcct tcaaacacgc tgcacaaaaa
    10501 gaaatgcttc ctttaccaaa aacaagttta caaatgatgg atgctgatct aaatctcatc
    10561 acaacgccta aactattttt ggaagaagaa cagctgccgg aaaattatcg aatcgttggt
    10621 ccaatttttg ctaagctcag tacttccttg cctgaagaag tgctaacttt tatacagcga
    10681 aagcaacaag aaagaaaacg tattatctat ttcgcaatgg gtagttcagg caatccgcgt
    10741 atgttacgca aaattttgtt cttcttacaa aaacaaccgg atctagcggt tattgcacct
    10801 attgactatc tgcttgatga cattcaatct gactcagaag atcttttaat aacagaatat
    10861 ctgtctagtc atcttttgca tgaattcatt gatttttctt ttatccacgg cggggaaggg
    10921 accgtccaaa ctgcctgtga atccggcaaa ccgtttatcg gtgtggggat gcaatttgag
    10981 caaagctgta atattgacta ttgtgtaaaa tacggaaacg caatcgccct cacaaaacca
    11041 gtgactgaaa gaaaactgca gattgctctg cgcgaagttt cttcacccaa gattcgtcag
    11101 caggcacgca cgctgcaagc cgtttttcca ataaatggtc cacaacaagc aattcaagaa
    11161 atcgaaacct tcctgagcaa ccacccaata tagtttcaag tagaaaaagc ttttttaata
    11221 gagactcatc agtaatttcg ctattcgaga cccagacagt gaaaatacaa aaaaagtaaa
    11281 tagtggattg aaaagttaga gttacttaaa ttacggtgtc taaaaaacac aaaatcatcc
    11341 ttaaaataaa agatctgacg ctatccaaat gagcataggt agcgtctgcc atttattctt
    11401 agtcaaattc tttgacacct ttctctggtt ggatcatctc aacgtttctt caaaagaaag
    11461 accataataa ttaggcaaat acattttctt aaaatgtgtc atatagatct agaagcatcg
    11521 ctttgtagag atttgtgccg ctagtcccct caacaattac taaatcaaat gtaggattta
    11581 ccactttatc tttgataatc aacctccctc ttctgttgag acaatgatct cgcaatccga
    11641 tgcatactcc tatataaact gccagcaatc cccacatatt gagtagatca tgtcagaatg
    11701 ttctatgtcg tcagcaacga tacactaaaa gtcacgttca ccatgactga ttgctattcc
    11761 cagcacaatt atttctatac acgtttcatg atttgaataa acactcacat catacatatt
    11821 accattcata caccatactg cagcccctac tatatggttg taagctccca aatctatatt
    11881 tgacttgatc actttggtag tagatctact aattctaaat ccattgctga taaaatttcc
    11941 attttttcct ccttctttct gccaaaacag ttaaaacaat aataatcttt atgtaaaaaa
    12001 gggctgtgac aaaagccttg tcacagtccc atattccgaa taaacggtgg tcaaaagctt
    12061 gcatctgcgg taataagttc aaccaaacga aaaataagcc aacatttcac aaaaatttaa
    12121 gaagcaattt tcgtaaattt cttcttattt cttgaagcta accacttctg ttacaaccac
    12181 ttttagaaca tttaaagctt cgatgtgaca aaccatctat gctgctcgat cataaaattg
    12241 atagcaaaaa ttccacaaag tacatatgtc ccccatgaaa acaaaacatt catatgcggt
    12301 acaatgatct ttggaagaat cacgatcaaa aatgatgcca gtgcccaaat ccatcgattg
    12361 atttgaatat gatatgtagc tagcatattc aatataaggt gactagccgt taataaaaag
    12421 ataaaaacaa aaaataaaat cagccaatac attgtttccc accttctaaa aaccaaactt
    12481 agacagtctg gacttcgttt tctccacgac tttgttcatc tgacagcatc tttttatcat
    12541 aagcttttac aaatggataa tagataataa atgctatcaa tgtgttgatc atgatcaaga
    12601 taacaacacg ccagtcattt cctgctgaaa gataaccgcc gattggagct ggtaatgtcc
    12661 atgttggcaa aacagtaaat ctattgacca tttctaattt gactgcaaaa taactgataa
    12721 tagtcaaaac taaaggaact aagttgaacg ggatgaacat gtaaggattc aacatcatag
    12781 gagctccaaa aattatgggc tcattgatat tgaagatacc aggaataata gaaaaacgac
    12841 caacctcttt caagaatttt gatttacaga agaatataaa caaaacagat accacgatcg
    12901 ttgctcctgc accaccaatt gttaccgtcc attgataaaa ctgttcagga gcgatatagg
    12961 gtaattgggt ggcagctgta ccatctgcca atgcctttgc attctcatcc aacatgatca
    13021 gccacattgg acgaacaaca gcccctacaa tactcatgcc atggatacca aatgaccaca
    13081 acaaatggat caaaaacatt ggtagtaaaa caccaagcaa attattccct aaaatattgg
    13141 ttaaaggctc gaataacgat aataaggcag cgtttacatc aaagcccgcg acgcttcgaa
    13201 tcagccaagt aattgttaaa atgactgcac ctggaatcaa tgcctcaaaa ctacgggcaa
    13261 cagcaggtgg cacttgttct ggcattttga tagtaatatt tcgattttta aagaaacgat
    13321 atacttccac tgcaaaaatc atagataaga tccccgcaaa catcccagat gctcccaaag
    13381 aactcatagg taagacccag ccaagaggtg cctcttcagg agttaaaacg tttacaggaa
    13441 cttgtaacat aaagaacgtt cctaaagata atataccacc tgtcacacta tctaatccat
    13501 atgatttagc tagatccgaa cccataacaa aagttgcata aatagccatc aatcccatcg
    13561 tcatcctaaa gggtaaaaca atcgttgccg aataaggggc aataaattca ttccatccag
    13621 gaaacggtaa attgataaag accataaaaa atgtcccaat caaaatcaat gacaatgttg
    13681 aaacaacccc attacgaatt gctaataaat gacgctgtgt accaattttt gccattggtg
    13741 ggacaagttt ctcttcgacc catttcatca gcttttccac taaaaccact ccttttcaat
    13801 aaaatatgaa ccattcattg catgacaacg caaagaatgt tcataactaa ttatataatt
    13861 aagaaatttt atttcaatat attctcttaa ataatcaaat ttatttttaa gaatcctgaa
    13921 aataagtttt ctaaatcata ggagcaacaa aaaaattcga taattaacaa aaatcaaaat
    13981 gattaaatct agtgattgaa acatatcatt tcaaaaaaat tttgtaaatt cacccataaa
    14041 tatcacccta tttaactttt ttcatcgtac actaaaattc aaaacaacta tctttaatct
    14101 ttacttcttg gttttgtttt aataaatttc cttcaaaaat gcctggagaa actacaatga
    14161 tcaccgtatc tttttgtgct tgctccaact tcttcaaatc tataatagct aacagtgtcc
    14221 cttcttttac ctcctggtta ctttcaaccc gtatctgaaa cggacttcct tcgagttcaa
    14281 ctgtatctaa acccatatgg atcagtattt ttctgccaga ttcactttcg atcgttatgg
    14341 catgtcgggt aggaaatata ttatcgatta ttcctttaac tggcgcatgg atatatccat
    14401 catgattcgt tatagcgtat ccaactccta tcatttttga agaaaagacc tcgtctttta
    14461 ctgctgttaa tggaataatt tgaccactag ctacagcttt tagaatgttt cttgttttaa
    14521 agaaatgcac tttttatcac ctctacttta atgctggcca atattttttg tttactttga
    14581 tcatttcatc tagtattttt tttgcgactc tggcatttgg tacggtttga ttcaatgtaa
    14641 atgcttgtaa agctttttga taagaacctt caaagtaagc atctactagt aacttctcta
    14701 ctgcaacttg tgcctccatc aatcctttat gaaaatcact gattggtttt cttagtgtaa
    14761 ttggctccac accagttgca ccaacatatg ccggtatttc aacaactgca tctgaccgta
    14821 gattcggaat cgccccttga ttaggaacga ttagcataaa gcgacgacga tcatcattca
    14881 acaaggaaat agctatatcg acgatatatt gtccatgctc cccaaaatta aagttcaata
    14941 cttcttccac aataccgcgg cgtattttat cagccatttc tttggtattt ttctctcgtc
    15001 catccatgac catattagca cgtgtatact ctttgtcagt atgctcaacg accatatcag
    15061 gatataagta atactctaag taattattcg ggatattact tggaaaattt ttcgtgatga
    15121 ctgacatcat ttgaaaggtt ttttgccaag ttttatctcc tatattaaaa tcggcgactt
    15181 gcatttcttt tgttattagt ttttcaataa tctctggcat aatttctcgt tgcaattctt
    15241 tatcataaat cgatgtatac cagccaaagt ggtttaaacc ataatacgta acgatccaat
    15301 tcttacgatc ataaccgtaa ttcactgcaa tcgtttcttc aattgaaata gtcatatcac
    15361 aggcattaat catttttaca ttcggatatt tacgacgaac agcttcagaa acgatcgact
    15421 ctggatttgt ataattcaaa atccatgctt tgggggcata gtcttgtata tgatcgacaa
    15481 tttccaataa acctttgatt gagcgcatcc cataagcaaa tcctccgagt ccacacgttt
    15541 cttgtcctac taatccatgt ttaagtggaa tcttttcgtc cttttctcgc atctccaatc
    15601 ctccaacacg aatttgtgag aaaacaaaat cgcatcctgt aaaagcaagc tttggatcat
    15661 ccgttgcacg aatgcgaata tttgaaaaac cttttctttt cagcataaat ttaacaatca
    15721 agtacatatc catatttctt tcttcatcaa tatcatataa acgtatttca gataatggca
    15781 accgtttttc attgttcaaa atggcttgaa cgattcctgg tgtatacgta ctcccgcctc
    15841 cagctatagt gataatctgt tttttcatca tcatcttttc ctttctattc ttctaataat
    15901 ttattgaatt ctgccataac ataagtcact tctaagccga tgataatttg ataatttttt
    15961 tcagttggtt taatgatccc gcttatccct aattgcttga ttcgctgttc ctcgaccatg
    16021 gtgggatcca ctacctctac ccgtaggcgt gtattacaac tatagacatc caggatattg
    16081 gcttttcctc caaacgcttg tagcattttt gttgccatat attcataatt tttacttgct
    16141 aaccctaact ctgtttcttc taaggttact tcattgccaa actctgcctt agcttcacgt
    16201 cctaatgtct gaatgtttct cttattgatc aaataataaa aagtaacgta atacaaggca
    16261 aaaaagaaaa ttccgattgg aagaatcaaa atagcattgg tagcgattcg aaaattgatg
    16321 agataatcaa caatacatgc tccgaatgta aaaccaattc gtatattgaa taagtaacag
    16381 acaatccctg ctaaaccggc aaaaagtgca tggatcacgt acaacatagg agaaagaaac
    16441 atgaaactaa attcaacagg ctcagtgata tttgcaataa atgaagttcc tgcaccgctt
    16501 cccatgattc ctttgaccat ctctttattt tctttataag ctgctttata gatagctaga
    16561 catatagctg gtataccaaa catcattact acaaaaaaac ctgataaaaa cagtccagcc
    16621 tgaggatctc cgttgataaa gcgtgtcatc tctcctctga aaacttcctt gctaggagat
    16681 gtatagcttc ctaaatcgta ataaatatac gtattcaaca catggtgcag tccaaagggg
    16741 atcagcaaac gattcaaaaa cataaatatc cccacaccta atgctcccat atcaacgagc
    16801 agttttgcaa aattatcaat cccaatttgg atgagcggcc agataaagcc caaaaaaaca
    16861 gctgcgattg tttgtagaat catcaccata gtcaatggga atttttcgcc cgcaaaataa
    16921 gaaaaaacta ctggtaattt ttgttcttta aagcgattat acgtccatgc cgctactaaa
    16981 ccggaaataa ttccaccaaa aatccccata ttcacttctg ggtccataat ttctaagcct
    17041 tttttcaagg tagcaatcgc taaaaaacca gtaagtgcag gtattccttt gtccttattt
    17101 ttgacaaaac cgattgcaac tcctatagca aataagatat ccaagtttcc aaaaacggca
    17161 tttccagctg caccaattac tgggatgttc agcatatcgt cagctgataa acgcatgatc
    17221 aatcccgcta atggcatcgc tgcaactgga agtagcatcg ctcttccaag cttctgcaca
    17281 tttgatttaa cattcatttt tattcctcct ctattaaata tcctcatatg ttactaactc
    17341 aatcaaagga tcatttttca ctttttgagc aaatgctgaa tgaattaaaa attcgacttg
    17401 gtgggtacga ggatacacaa aacttgaacc atttaacaat tcctgatcca aatacgctgc
    17461 gtgacacatg acttcgatcg ttttatcttc tctcaatatc atgagtaaac ttgccaaata
    17521 ttcttccaca ctcgtattga tttgtcgttt tttttcatca gtttctccta cgacatcgaa
    17581 acaaggctca aaataatcga catgacgaac ttcatctttt cgttcaaagt tgctccgaac
    17641 cggcaaatca tatttttttg ctaatgtaac aactacctcc tgattttcat ccaaagtatg
    17701 agtatgatga tgactgtcta aatgagttgg tatgatcccc gcatgataaa ttttttgaat
    17761 ctgagcattc cattcctgat acagttgatc tttgtcaatg ttaaatggat gctgataata
    17821 agataaggat ctgaactctc cgctttcgat aagagtatcg acagttttga ggagtggttt
    17881 gccacacgtc aatgttaaat ggactcctac acctagccta ggcatttcct ttcttagttt
    17941 cacagcatgc tcgaaacccg gcatatttgc catcaatgta gttgatgtca aaattcctcg
    18001 ctgataagca tccataatcc cataattcac accatggcta tagccaaaat catccgaatt
    18061 aaaaatcact tttcccattt cattctcctt cttttttcta ctaaaatagg cgcaaccaaa
    18121 taatcatagt ccatttacta tgtgtcattt ggttgcgcct atagatagtt acgcccttat
    18181 tttaatagat tcttgctaga tgaattgcca aatagatgat ttcattttct tgaatggcaa
    18241 gttgaaattg ctgttcgatg atatctttaa tctttagtgc tgtacaatac gctttgctat
    18301 gtttaaatgt ttcagtaaaa aaaccaatat tttctactgg cgtttcttcc tgataaatca
    18361 agcgtttaac caggaatttg atatgaacca ttaagcgatg ataagtcagt gattctttat
    18421 cataaacgtt tgaataggat ttttctagat gggacaaaat cgtggtcgtc atttctacta
    18481 tttttaagct ctttaaacta ttaggctctc ctagctcagc attaatgata tgcatagtga
    18541 tgaatcctac ttcatttgac cctaactcgg tttgatatcg atgattgatt cgctcgacca
    18601 cttctactgc tatatcatat tcatttggat aaaggaatct catttctggc aacataattg
    18661 ctgtgacatc tctatcttct ctttttcgtt gaatagcaaa ataaatatga tctgtcaatg
    18721 taatatagat atttgaatct aatggagagg agatcctagc tgttatcagt tcaatcactt
    18781 cttccgcaaa atccataaat tcgattggaa tctgcgagac tatctcactg aatcgtttga
    18841 tcttttcttt agaatctaat acaaataatt tgtcagcatc ctcaacatca aatggcttac
    18901 tcactttagc agaaaagcca atccctttcc ccatcgcaat tttttctatt ccatcgtcat
    18961 cgatcaatac agcattatga ttcagctttt tcatcacata caccgttaat tcacctcctc
    19021 tatatgataa aacaaaacaa cttgcagact ccttcataca tatttttgtc tccaaacaat
    19081 ttttaaagac aaaaaacgac ctaagaaaga accctttctt aggtcgtgcc taattgaata
    19141 gtagcaccct acataattta caaaaatagt ttaacacata ataaaagcgt tatcaatatc
    19201 ttttttactc gttttatgat aatacttgta tcgaattgat ttggaggtaa ttacataaaa
    19261 acaaaaaaaa tatagcaact ctctataaga aagttgctat attgataatt ataccggcgg
    19321 ccggggtcga accggcacgt cctcaacgga cactggattt tgagtccagc gcgtctgcca
    19381 attccgccac gccggcaaat ttaaaaataa taactggggt agctggattc gaaccaacgc
    19441 atgagggagt caaagtccct tgccttaccg cttggctata ccccaagaat aaagggcgaa
    19501 tgatgggaat cgaacccacg aatgccggag ccacaatccg gtgcgttaac cacttcgcca
    19561 cattcgccat cataaaagat gttatttaat ttctcaataa aaaactaaga tgaagacatc
    19621 ttagtcaata ccggcggccg gggtcgaacc ggcacgccct caacgggcac tggattttga
    19681 gtccagcgcg tctgccaatt ccgccacgcc ggcaagttga tttattagta ctaaggcggt
    19741 aaccggattt gaaccggtga tgaaggtttt gcagacctct gccttaccac ttggctatac
    19801 cgccattata ctattatcaa tctataaaat aaagaataaa ttatagaaac tggggtagct
    19861 ggattcgaac caacgcatga gggagtcaaa gtcccttgcc ttaccgcttg gctatacccc
    19921 aagaataaaa agggcgaatg atgggaatcg aacccacgaa tgccggagcc acaatccggt
    19981 gcgttaacca cttcgccaca ttcgccatgg caggggcagc aggaattgaa cccacactaa
    20041 cggttttgga gaccgctgtt ctacctttaa actatgcccc tataaaatgg aggagagtgg
    20101 attcgaacca ctgaacccta aggaacggat ttacagtccg tcgcgtttag ccacttcgct
    20161 actcctccat ggtggcgcgg gacagaatcg aactgccgac acacggagct tcaatccgtt
    20221 gctctaccaa ctgagctacc gcgccatatt ttaaaattat taattgaata acaagaacga
    20281 cggttccgac gggaatcgaa cccgcgatct cctgcgtgac aggcaggcat gttaacccct
    20341 acaccacgga accagttcat tatggaggtt aacgggatcg aaccgctgac cctctgcttg
    20401 taaggcagat gctctcccag ctgagctaaa cctccaacat tcaattaaaa tgacccgtac
    20461 gggattcgaa cccgtgttac cgccgtgaaa gggcggtgtc ttaaccgctt gaccaacggg
    20521 ccaggtaaat ttgctacgga gagtaaggga ttcgaaccct tgagacagtt tggcaccatc
    20581 tacatgattt ccaatcatgc tccttcggcc tctcggacaa ctctccagag tgaaagaagc
    20641 tcaatactct ttctcaaaat gag
//
LOCUS       DACGLW010000048        20263 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.050, whole genome shotgun sequence.
ACCESSION   DACGLW010000048 DACGLW010000000
VERSION     DACGLW010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20263
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.050"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            208..2250
                     /locus_tag="IXT53_001790"
     CDS             208..2250
                     /locus_tag="IXT53_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ4905598.1"
                     /translation="MNKRIREILKLIIKNPEMKLSTLTSELDLTRRQINYAINQFNEE
                     LKTKNIPLIQRSHSGNITVPIEVIQMISQPDYDIGDDQELVVLTDSERQALIVMTLIA
                     NIDYISLDHLVDAVDVSKTTVMEDIKRTEPFIRNYSLTIRYNRMNGYQLVGSEHRILQ
                     LLSDLVKRFPIFRREMIRRKLAQDVSEEEIVHLIHNMEQMLHLSYSDESVDYLQTSAR
                     FLVSRGIKMSDKHAFFPDDVTDTPEFRMLMILVHETGWSLSKSYMEWFTLLFLTSNIF
                     ESKTTQNYDSDQELRKLISQMVENFQNQTLILIDDREDFERRILSHLRPACFRIRYNL
                     SLGVYSIDSLIKDSNHAILIDLMKELIIPIENWLNKAFPNDELDLLSYYFGYQLSNHN
                     KLNKQRQRAVVVCTNGVMVSKLVRANMEKLFPEIHFLASLSVRDFYKFEADYDLVFTT
                     TPLKSSMLQFMIEPIMTYQQQISLRYRVLSDLGITKVDRAMDELLTIIRKYANITNPV
                     SLREELQYFLIKEDGSTPLDNFNVLPSLTYYLRPSYVQIVEEEMSWERAVEVACRPLL
                     EHQIIDSRFIADCLDQIRQPGYAGYLGMKTCIPHTTVDKGVISDGVSILVCKKPIRFP
                     NGEDVSLILPLSFFDLTKHLRAINQIADIAKDDSLIEQLLIADDEKTVYQLLRQRS"
     gene            2261..2881
                     /locus_tag="IXT53_001791"
     CDS             2261..2881
                     /locus_tag="IXT53_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HAQ4905599.1"
                     /translation="MRLADYPKLTVIMRGYTFDQADAILQAMQEFDHQFAVEMTMNTK
                     GALENIHELNQRYGDTTYIGAGTVRTLEQAQACYEAGAKFLLGPHMFTKEMLSYANKK
                     GLLAVPAAMTPSEVDRMFKEGADIVKVFPAAVVTPRFFKDIQAPLGKLPLMGVGGISK
                     ENAKEFFEQGASYLGLGSGMFNKQDIEELNVKNLARSMKELIEAIK"
     gene            2893..3351
                     /locus_tag="IXT53_001792"
     CDS             2893..3351
                     /locus_tag="IXT53_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ4905600.1"
                     /translation="MELYLNEELVFRNVQAKTDSEVLAFLASEMYKKNYVKEEYIQAI
                     QEREKEYPTGLPSTPPGIAIPHANYEMVNKTTLAIATLKDPVLFHNMEDNNEQLPIQI
                     VIMMAIGEPHGQVEMLQKIVGIIQDEPLRQEMIRAGNDTELLELLKKAVF"
     gene            3370..3648
                     /locus_tag="IXT53_001793"
     CDS             3370..3648
                     /locus_tag="IXT53_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ4905601.1"
                     /translation="MKRILVACGNGIATSTVVATKVREYLQQHGIDVSTTQTKLMEVP
                     GKVQDYDLLVTTGQFDGQTGGVPVIKGMPILTGIGADQTMEEILNLLK"
     gene            3673..5040
                     /locus_tag="IXT53_001794"
     CDS             3673..5040
                     /locus_tag="IXT53_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002386123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAQ4905602.1"
                     /translation="MKIIMNVFNFFIDAGPTVMLPVIITIIGLIFGLKISRAFKSGLT
                     LGIGFAGIKLILDFMTTNVGPAAKAMVDRTGVKLDALDVGWGSIAAVTWASPIIPILI
                     FAILLVNIVLLILKRTHTLDVDIWNYHHMAIVGVIVYFVTKNVFLGVGASVVMAIATF
                     KISDWSQPMVESFFGIPGVSLPTVSALSSLVIAWPLNWLLDRIPLFRKSKFTIKDAQK
                     YLGFFGDSMIMGLVIGMVIGVLAGYDIKAVLQLGVSMSAVLVLIPKMTSLFMEGLMPI
                     SDAAQKWSQKKFKGTRLFIGLDAAVVVGNPDVITTALIIIPLTIALALVLPGNRVLPF
                     ADLAVVPFRVAMVVALTRGNLLKNIIIGLVVTASLLWCGSATAPMLTAIAKSVGINLG
                     TSSMLISSFAATSMIQSYLVFIAFAYKPIIGIPVLLLAFAGAWYYFDGIKKVNHEMDE
                     PLKAQ"
     gene            5055..6200
                     /gene="dgoD"
                     /locus_tag="IXT53_001795"
     CDS             5055..6200
                     /gene="dgoD"
                     /locus_tag="IXT53_001795"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HAQ4905603.1"
                     /translation="MKISDVTVYKVKPRWIFVKISTDEGIEGWGEMISGTKTETVVAG
                     AYEIGNRLIGRNPFEIERLFQEMHRSFFRGGPINGTIVSGLEMALWDIKGKAFNVPVY
                     ELLGGAARDKIKVYSWIGGDRPSEVAEQAQDRFDRGFTAVKMNATEELHYIDSYQKVD
                     EVVERVASIRERFGDAMDIGVDFHGRVHKPMAKVLAKALEPFHPMFLEEVVLPENEEH
                     FKEVADSVAVPLATGERLYTRWQFKNLFKQGTVDIIQPDVALCGGILETRKIAAMAEA
                     YDMAVAPHAPYGPVALAATLQVDSCTPNVFIQEQSLGIHYNKGFDLLDFVKNKEVFQY
                     KDGYVDLPSKPGLGLEMDEDKIKEIAQEGLIWTNPQWKNYDGTIAEW"
     gene            6227..6718
                     /locus_tag="IXT53_001796"
     CDS             6227..6718
                     /locus_tag="IXT53_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905604.1"
                     /translation="MAEALMITVFLGLLIILQIIDQKKIIVFAQSSPVRKSLSILLTV
                     AILLIFWPEHLTDQIKLVAFASVILINGFLKEGLAKDSVVRFGVLNGNFNRYPQVQVE
                     KLGDDKSFISFYKSKNNSFSMIFDKDQTEMMDVFQELDIHSKITLDQSSEEKRAVVKK
                     DNE"
     gene            7161..8360
                     /locus_tag="IXT53_001797"
     CDS             7161..8360
                     /locus_tag="IXT53_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ4905605.1"
                     /translation="MRDQTSGKMIRFYYTSLHFLYWMMFCSVYGFATLFLIYFKIDPA
                     KIGGILALVNIVSTVLQPFVSQVIIKKRKIPLITVLKTMIAGLGVILLAGVLIPNLPI
                     ITYILGGILVVSMQSFLNALAFEYSNTGYRINFGFSRAGGSFSYAVTSFLLGQLLAIY
                     SAAILPILVIVEAVIFFVILHLLPDVKNQEVFPMTKEQDRSVSLRKKYPFLILLFLGF
                     SFLFTFHTMINSFLAQIFENIHGGSKEVGIALMIAALCELPGMIFFEQLVKKKSSKFW
                     LSVASISFAVRGFMMLLASSILMMEMTHLLQAFSFALFIPASAYLFNQFLADDDRVFG
                     QTMIIIATTLGGVIGNVLGGSLLQHFGVWEMLIFGTGFAVLGALLTILGIRKIPKTGN
                     DSDSLVE"
     gene            complement(8855..9613)
                     /locus_tag="IXT53_001798"
     CDS             complement(8855..9613)
                     /locus_tag="IXT53_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ4905606.1"
                     /translation="MDTKLSESEKYLWDFIQSHILEIPSYSIVKLSEYANVSTATVVR
                     TMKKKGYEGFTSFKHSLKEKENTNINFAALDKVDEGIKTAILKNEQEVIRTINMVESG
                     NIEDAIQRIKAARRIMIFARGFSELIAQEMMVKFQLTGKYCEMHTDPNIIKNISRKLS
                     KDDIVIFVSLNGETKELTTAAENCYKAEIGTILLTANRHSSLMEHIEIPFVGFKSEGS
                     FFPDYEVRSRLPLSILARILLDSYALRTSGFTQH"
     gene            9781..10716
                     /gene="pfkB"
                     /locus_tag="IXT53_001799"
     CDS             9781..10716
                     /gene="pfkB"
                     /locus_tag="IXT53_001799"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HAQ4905607.1"
                     /translation="MSVYTCTLNLAIDLFIETDEMKPSQVNRTNDDDIQANGKGVNVS
                     LIMKQLGIDSTALGFKAGFTGNYIEDYLHQKDISTDFISVPGMTRINVFTQVNKTNEE
                     FKLVNKGPEIPQEQIKTFLNQIQKLQADDYLCVSGSLPRGVSPEILIDISRICFENKV
                     HLIIDSSYPEIIDCLPYQPFLLKPNEEELQSWYNVELSDREGYIHYAERLIADGAKNV
                     LLSLGGEGGIFLNKDIVYSGNSPKGKVVNTACAGDTLLGTFLAGYLKGEQVDINLKKS
                     LAAGSSTAFRKGLTDFSDVAELEKQIEIKEGASKK"
     gene            10713..12158
                     /locus_tag="IXT53_001800"
     CDS             10713..12158
                     /locus_tag="IXT53_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ4905608.1"
                     /translation="MTTYKIVAATGCPTGIAHTYMAQEALEQAAKRKGVSIKVETHGQ
                     VGIENELTPEEIKNADAVIIAADKDVQAERFAGKRVIDVPVSKGIKDADQLIEDALAG
                     KGSITAENKTVDAAGEKAEMGVAAGIGHSIYKNLMNGVSHMLPFVVAGGVIIAISFAI
                     WGVYSFDPNDPTYNPTAAMLKGVGDAAMGMMVPILAAYIAEGIAKRPGLVVGFVGGLL
                     ANSGGAGFLGGIFAGFLAGYFILLLQKVMKGLPKQLDGLKAIFLYPVIGVLFVGVVMS
                     LLANPMEAVNQGMMNFLAGFQNSNPLILGIIVGCMSAFDMGGPVNKAAYVTGTALLAQ
                     GNHTFMAGVSAACIAPPLITGFAALFFGKYFDTNDRNAGLVNFILGSTHITEGAIPFA
                     AKDPLRVLPTMMLGSSIAAVLTYMFGVQVPAPHGGFLVLPVVTGKVAWVGSILLGSIV
                     GGVILGLIQKRSVEKREAEAAAVEMKEATNN"
     gene            12186..12641
                     /locus_tag="IXT53_001801"
     CDS             12186..12641
                     /locus_tag="IXT53_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ4905609.1"
                     /translation="MTLIKTNKVFMNKNFTTQDEVMNFLADQSIVLSISEDRDAVYEK
                     LMEREEESTTGMMDGFAIPHAKDASIKDADIVIVRLNGKVDWKSLDGEGTDFVIALFI
                     PDSEAGTTHLKFLSQVARLLMHKDVTEGLKQAQSAEEIAELLNQKLEEN"
     gene            12660..13634
                     /gene="lacD"
                     /locus_tag="IXT53_001802"
     CDS             12660..13634
                     /gene="lacD"
                     /locus_tag="IXT53_001802"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAQ4905610.1"
                     /translation="MPLTEKKKTYIRQLSNESGIISALAIDQRGALKKMIGKFQNKSA
                     TDEQIINFKKIISSELTPYASAILLDPEYGLPAAEVRDKNAGLLLAYEKTGYDASTPG
                     RLPDILSIWSVKRLKEQGADACKFLLYYDVDESDDINDQKKAFMERIGSECVAEDLPF
                     FLELVSYDANDPDTSSKEYALKKPHKVNEMMKEFSKPRYGVDVLKVEVPVNMNYVDGY
                     TKNEVAYTKEEAAAYFKEQSETTNLPFIFLSAGVTAELFQETLKFAKEAGSTFNGVLC
                     GRATWANGVEPFVTKGEYAAKEWMQTQGRKNIEELNNVLKETATAIKL"
     gene            complement(13745..14306)
                     /locus_tag="IXT53_001803"
                     /pseudo
     CDS             complement(13745..14306)
                     /locus_tag="IXT53_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364878.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
     gene            14450..15064
                     /locus_tag="IXT53_001804"
     CDS             14450..15064
                     /locus_tag="IXT53_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ4905611.1"
                     /translation="MELAFKQLEKKDYKKAIQFAIKGMHFDWYTKNKLLLNLYGRYFW
                     YLELNRATQIIALYADDELAGVLLADIKGKSKKHHSFSQKLYVKLFDFLQNAFVKDSK
                     GGYDDTNDELLSDYLKKHSPDGEIVFLAANPDLKIKGIGSKLLKEFERREQGKEVFLF
                     TDSGCTYQFYERRGFERKKEKDNVMKILNKEVDLRCMLYSKIIQ"
     gene            15305..15688
                     /locus_tag="IXT53_001805"
     CDS             15305..15688
                     /locus_tag="IXT53_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ4905612.1"
                     /translation="MPKYKEIALALKERIVEGKYQEGSKLPEQESLAREFKTSRVTIR
                     KAIQLLIDEGLLYTRRGSGTFIRSNIRQDNENVTQVNNVFGTSSQENTNVTSKIIRFD
                     VRFPTDYERRLSATYFRSPSCKSWV"
     gene            16051..16560
                     /locus_tag="IXT53_001806"
     CDS             16051..16560
                     /locus_tag="IXT53_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HAQ4905613.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILEVFAEAELYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            16660..17310
                     /locus_tag="IXT53_001807"
     CDS             16660..17310
                     /locus_tag="IXT53_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905614.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEVRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNTNHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            17761..18699
                     /locus_tag="IXT53_001808"
     CDS             17761..18699
                     /locus_tag="IXT53_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAQ4905615.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            18712..19764
                     /locus_tag="IXT53_001809"
     CDS             18712..19764
                     /locus_tag="IXT53_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ4905616.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            19822..>20263
                     /locus_tag="IXT53_001810"
     CDS             19822..>20263
                     /locus_tag="IXT53_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HAQ4905617.1"
                     /translation="MTYVFLPKYGFYFLLLGVISDLSTPYILGLFYPKLNQMTTVISV
                     FGDVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFVGTSKTLAILAVATVGLYGIGDCI
                     FTGLFSINTNESSWNLSTWIHNTGSGLGYAGFLLFPLLLVLLYRQ"
BASE COUNT         6421 a         3221 c         4277 g         6344 t
ORIGIN      
        1 catccaatat tctaccgaat atttaatatt cacattagcc ctaaaataat tgtcatacca
       61 tgttacattt gatataacag cgttcatttt aaaaatagct gttagtgtga caaaacatta
      121 aaccaagtgt gacaaaaaac gtgatgaaag ctttccggaa acgtatgtga acaagtatat
      181 aatgattttg tcaggaggat agtcaaaatg aataaacgaa ttcgtgaaat actcaaactg
      241 attattaaaa accctgaaat gaaactttcc actttaacaa gtgaacttga tttgacacgt
      301 cgtcaaatca attacgcgat caatcaattt aatgaagaat tgaaaacaaa gaacattccg
      361 ttaattcaga gaagtcattc tggaaatatt acggtaccaa tcgaggttat ccaaatgata
      421 tcacagcctg attacgacat aggtgatgat caggaacttg tcgttctcac tgatagtgaa
      481 agacaagcat tgatagtgat gactctgatc gctaatatcg attacatcag tctggatcat
      541 ttggtagatg cggttgatgt tagtaagacg actgttatgg aagatatcaa acgaacggaa
      601 ccgttcatca gaaattatag tctgacaatt cgatataatc ggatgaatgg ttaccaattg
      661 gttggttcgg aacaccgtat cttacagctg ttatcggatc tagtaaaacg gtttccgatt
      721 tttcgccgag aaatgattcg cagaaaattg gcacaagatg tttctgaaga agaaatcgtc
      781 catttgatcc ataacatgga acaaatgttg catcttagtt attctgatga gtcggtagac
      841 tatttgcaga catcagcgag atttttagta agccgtggga tcaagatgtc agataagcac
      901 gcgttttttc ccgatgatgt gacggatact cctgaattcc gaatgctgat gatcttggta
      961 catgaaacag gttggagtct atccaaaagt tatatggagt ggttcacgtt attattttta
     1021 acttccaata tttttgagag caaaactact caaaattatg attcggatca agaattgcga
     1081 aaattgatca gccaaatggt tgaaaatttc caaaatcaaa cgttgatttt gatcgatgac
     1141 cgagaagact ttgaacgtag gatactgagt catctgcgtc cagcctgttt tcgaatacgc
     1201 tataacttga gtttaggagt ttattcaata gatagtctga tcaaggatag taatcatgcg
     1261 attttgattg atttgatgaa agaattgatt attcctattg agaattggct aaataaggcc
     1321 tttccaaatg atgaattaga tcttttatcc tattactttg gctatcaatt aagtaatcat
     1381 aataaattga ataagcaacg acaaagggct gtagttgttt gtactaatgg tgtcatggtc
     1441 tcaaagttag tacgagccaa tatggaaaaa ctttttccag agatccattt tttagcttcc
     1501 ctatctgtgc gtgatttcta caaatttgaa gctgattatg atctggtctt tactacgacg
     1561 ccactaaaaa gttctatgct gcagtttatg atcgagccaa tcatgacata tcagcaacaa
     1621 atcagtttac gatatcgagt tttgagtgat ttaggaatta ctaaggttga tcgagcaatg
     1681 gatgaattgt taacaatcat taggaaatat gcaaatatca ccaatccagt ttcgttgcga
     1741 gaagaactgc agtacttttt gatcaaggaa gatggaagca cgccgctgga caacttcaat
     1801 gtattgcctt cattgactta ttacttgcgg ccgagttatg tacaaatcgt tgaagaagag
     1861 atgtcttggg aacgagcagt ggaggtggct tgccgaccgt tattggaaca tcaaatcatt
     1921 gactcgcgtt ttatcgctga ttgcttggat caaatcagac aaccgggata tgcaggttac
     1981 ttgggaatga agacttgcat tccccataca acggtcgata aaggtgtaat cagtgacggt
     2041 gtcagcattt tagtttgtaa aaagccgata cgatttccta atggtgaaga tgtcagcttg
     2101 attttgccgc tttcattttt tgatttaacg aagcatctgc gagccatcaa tcaaatcgct
     2161 gatatcgcca aagatgattc attgattgag cagttattga ttgctgatga cgaaaagaca
     2221 gtttatcaac tattaagaca aagatcttaa ggaggaaaac atgagactag cagattatcc
     2281 taaattgaca gtgatcatgc gtggttatac ttttgatcag gcagatgcta ttttacaagc
     2341 aatgcaggaa tttgaccatc aatttgccgt agagatgaca atgaacacaa aaggagctct
     2401 tgaaaatata catgaactaa atcaacgtta tggtgacacg acttatatcg gcgctgggac
     2461 ggtaaggaca ttagaacaag cccaagcctg ctatgaggct ggtgcaaaat ttttattagg
     2521 accacatatg ttcacaaaag aaatgttgtc ttacgccaat aagaaaggat tattggcggt
     2581 acctgccgca atgactcctt cagaagtcga ccgtatgttt aaagaaggag ctgatattgt
     2641 aaaagtattt cctgcagcgg ttgttactcc acgttttttc aaggatattc aagcaccttt
     2701 aggtaagtta ccactaatgg gggttggtgg aatcagtaag gagaatgcga aagagttttt
     2761 tgaacaaggt gctagctatt taggtcttgg ttccggtatg ttcaacaagc aagacatcga
     2821 agagctaaat gttaaaaact tagcacgttc gatgaaagaa ttaattgagg ctattaaata
     2881 ggagtgagga ctttggaact ttatttgaat gaggaactcg tttttcgaaa tgttcaagca
     2941 aaaacagata gtgaagtctt agcgttttta gcaagcgaga tgtacaaaaa aaactacgtg
     3001 aaggaggaat atattcaagc aatccaagaa cgggaaaaag aataccctac tggattacca
     3061 tcaacaccac caggaatcgc aattccccat gcaaactatg aaatggtcaa taaaacaact
     3121 ttagcaattg cgactcttaa agatccggtc ctatttcata atatggaaga taacaatgag
     3181 cagttaccaa tccaaattgt gatcatgatg gcgattgggg aaccacatgg gcaagttgaa
     3241 atgttacaaa aaattgttgg aatcatacaa gatgagccac tgcggcaaga aatgatcaga
     3301 gcaggcaatg atactgaatt attagaacta ttaaagaaag cggtttttta atataaggag
     3361 gaaatcataa tgaaacgaat tttagtagca tgcggaaatg gtatcgccac ttcaacagta
     3421 gtagcaacaa aagttagaga gtatttgcaa caacatggca ttgatgtttc aacgactcaa
     3481 acaaaattga tggaagttcc aggaaaagtt caagactatg atctattggt gacgactggt
     3541 caatttgatg gtcaaacagg tggtgtacca gtaatcaaag ggatgccgat tttaacaggt
     3601 atcggtgccg atcagacaat ggaagagatt ttgaacttac tgaaataata gctgatcaaa
     3661 ggagctgaaa aaatgaaaat cattatgaac gtctttaact tctttattga tgctggacca
     3721 acagttatgt tgccagtcat catcacgatt attggtttga tttttgggtt aaagatttct
     3781 cgtgctttta aatcaggttt gacattaggt atcggttttg ccgggatcaa gttgatattg
     3841 gactttatga cgaccaatgt tggaccagct gccaaggcga tggttgatcg aactggtgtt
     3901 aaactagatg ctttggatgt tggctggggt tcgattgcgg ctgttacttg ggcatcgcca
     3961 atcattccaa tcttgatttt tgccattcta ttggtcaata tcgttttgct gattttgaaa
     4021 cgtacacata cgctggatgt tgatatctgg aactatcacc atatggcaat cgttggtgtt
     4081 atagtgtatt ttgtgactaa gaatgtcttc ttaggtgtag gtgcatcagt agtgatggca
     4141 attgcgactt ttaagatttc agactggtcg caaccgatgg tagaaagttt cttcggaatc
     4201 cctggtgttt ctttaccaac tgtttctgcg ctttcatcac tggttattgc ttggccgtta
     4261 aattggctgc tggatcgtat cccgttattc agaaaatcaa aattcaccat caaagatgca
     4321 caaaaatatt tgggattttt tggtgattct atgatcatgg ggttagttat tgggatggtg
     4381 attggtgttt tagccggcta tgatatcaaa gcggttttac aattaggtgt cagcatgtca
     4441 gctgttttgg ttttgattcc taagatgact tcattattta tggaaggatt gatgccaatt
     4501 tcagatgctg cacaaaaatg gtcacaaaaa aaattcaaag gtacacgttt atttatcgga
     4561 ttggatgctg cagttgttgt aggtaatcca gatgttatca caacagcatt gatcattatt
     4621 ccactaacga ttgcattagc tttagtattg ccaggaaacc gtgtgttacc attcgcagat
     4681 ttagcggttg ttcctttccg cgtggcaatg gtcgttgcct tgactcgtgg taatttatta
     4741 aaaaatatca ttattggctt agttgtgaca gcttcattgc tatggtgtgg gtcagctact
     4801 gcgccaatgc tgacagcaat tgccaaatcg gttggtatta acttaggaac ttcttcaatg
     4861 ttaatctcat catttgctgc gacttccatg atccaaagtt atttggtatt tatcgcattt
     4921 gcttacaaac caatcatcgg tattccagtg ttgttattag cctttgcggg cgcatggtat
     4981 tacttcgatg gtatcaaaaa agttaatcat gaaatggatg aaccattgaa ggcacaatag
     5041 taggagggtt taaattgaaa atttcagatg tcacagtgta taaagtaaaa ccaagatgga
     5101 tttttgttaa gatctcgact gacgaaggaa tcgaaggctg gggagaaatg atctcaggaa
     5161 ctaaaacaga aacggttgtt gctggagctt atgagatagg aaaccggctg attggacgta
     5221 atccgtttga aatcgagcgc ctcttccaag aaatgcatcg atctttcttc cgaggcggtc
     5281 ctattaatgg aaccatcgtt tcgggtttag aaatggctct gtgggatatc aaaggaaaag
     5341 cgtttaatgt tccggtttat gaattattgg gcggtgcagc acgtgataaa atcaaggttt
     5401 attcttggat tggtggagat cgaccaagtg aagttgcaga acaagcacaa gatcggtttg
     5461 atcggggctt tacggccgtc aagatgaacg cgacagaaga gctgcattat atcgattcct
     5521 accaaaaagt tgatgaagtg gtggaacgag ttgcgtctat tcgcgaacgt tttggtgatg
     5581 caatggatat tggtgttgat tttcatggac gggtccataa accgatggca aaagtgctag
     5641 caaaagcttt ggagccattc catccaatgt ttttggaaga agtcgtttta cctgaaaatg
     5701 aagaacactt taaagaagta gctgattctg tggctgtacc attagctact ggtgaacgct
     5761 tgtacactcg ttggcaattc aagaacctct ttaaacaagg tactgttgat attatccaac
     5821 cagatgtggc tctttgtggg ggaatcttgg aaacccgtaa aatcgcagcg atggcggaag
     5881 cttacgacat ggctgttgcg cctcatgcgc catatggtcc tgttgcacta gcagcaactc
     5941 tgcaagtaga ctcatgtacg ccaaacgtct ttatccaaga acaaagctta gggatccact
     6001 ataacaaagg ttttgatctg ttagattttg tgaagaacaa agaagttttc caatataaag
     6061 acggctacgt agatctacct tcaaaaccag gtctgggctt ggaaatggat gaagacaaaa
     6121 tcaaagaaat tgcacaagaa ggtttgattt ggaccaatcc gcaatggaaa aattatgacg
     6181 gtacgatcgc tgagtggtga taaatgaaaa taaaggatga tgaaagatgg cagaagcact
     6241 tatgatcaca gtttttttag gactgctgat tattttgcaa ataatcgatc aaaagaaaat
     6301 tatcgtgttt gctcagagta gtcctgtaag gaagagtttg agtattttac tgacagtcgc
     6361 catcctgttg attttctggc cggaacatct gactgatcag attaaactgg ttgcttttgc
     6421 ttctgtcatt ttgatcaatg gttttttgaa ggagggtcta gcaaaagatt ctgttgttag
     6481 atttggagta ttgaacggta actttaatcg atatcctcaa gtccaagtag aaaaactagg
     6541 tgacgataaa tcatttattt cattttacaa aagcaagaat aattcttttt caatgatttt
     6601 tgataaagat caaactgaaa tgatggatgt ctttcaagaa cttgatatcc attccaaaat
     6661 tactttagat caaagttcgg aagaaaaaag agcagttgta aaaaaagata atgaatgatt
     6721 ctactaccga aattatgatt tctttatcac gaataatgaa acttgtttta atgaatcata
     6781 ttgattgaag tatacaatgt atattaagtt ttggaaagaa atatattggt acaagatata
     6841 gcaggaatcg attactcgat ccctgctttt tttaacttcc tttgcagcaa aaaaagctcg
     6901 taatctaaga gatgatggat agtttttaga atatatatat tttcttcaat tccagaaaaa
     6961 aatgaccaag ctagtatgct aaaaaagtac gatgcttcta tgatcttttt tccgtcttta
     7021 ttaacagatg ttttaattga tcaacgcata ttgtaatcta ccctaatttc catagttctt
     7081 caccactttt aaaaatagta aaacccctac aagtatgcta tagtaaattg ctaaagaaaa
     7141 accaataagg tgggagatgt gtgcgcgatc aaacaagcgg aaaaatgatt cgtttttatt
     7201 atacaagttt gcattttctt tattggatga tgttttgttc tgtctatggg tttgcgacat
     7261 tgtttttgat ttatttcaag attgatccag ctaaaatcgg agggatctta gcattggtca
     7321 atattgtttc aacggtgctt cagccttttg tcagtcaagt gatcatcaag aaaagaaaga
     7381 tcccactgat caccgtgctg aagacgatga tagcaggatt aggagtgatt ctcttagctg
     7441 gggtattgat tccgaatttg ccgatcatta cttatatctt gggtgggatt ttagtagttt
     7501 ctatgcaatc atttctcaat gcgctggcat ttgagtattc aaataccggc tacaggatca
     7561 actttggctt ttcaagagct ggtggttcat tttcttatgc tgtaacttct tttttactgg
     7621 gacagctgtt agcgatttat tctgccgcga ttttgccaat cttggttata gttgaagcag
     7681 tgatattttt cgttattttg catctgctgc cggatgtaaa aaatcaagaa gtttttccta
     7741 tgacaaaaga acaggatcgc tcagtttctt tgagaaaaaa atatcctttc ctgattttgt
     7801 tatttttagg tttcagtttt ctttttactt tccacaccat gatcaacagc tttttggcac
     7861 agatctttga aaatatccac ggcggcagta aagaagttgg catcgcattg atgatcgccg
     7921 ctttatgcga attaccggga atgatctttt ttgaacagct tgtcaaaaag aaatccagca
     7981 aattttggct gtctgttgcc agtatttcat ttgctgtcag aggattcatg atgctgctgg
     8041 ccagttcgat cctgatgatg gaaatgacgc atctcctaca agccttcagt ttcgctttgt
     8101 ttattccagc ttcggcttat ttgttcaatc aatttctggc ggatgatgat cgtgtatttg
     8161 gacaaacgat gatcatcatc gccacaactc ttggtggggt gataggaaat gtcttgggcg
     8221 gcagtctgct gcagcatttc ggtgtatggg agatgctgat tttcggtact ggttttgcgg
     8281 tgctaggtgc tttattaact attttgggca ttcgaaaaat accgaagaca ggaaacgata
     8341 gtgattctct tgttgagtag cataaaaata cttagatact agttttatat aaataacata
     8401 tagtgattat tggcgtaatt acaaaaaacg agaataactt aagtaagcat ttctaaataa
     8461 ttgagaaacc tcatctaaaa agataagaaa atatccctaa taaaacttct gtaacatttt
     8521 agataactga ttgtaatctg aataataaac tataaggaga cggttagacg gtctcctttt
     8581 tttgcgtatt ataattctag aattgttgga aattgataat aattaacaag aaatattaga
     8641 caggaacttc gaactgctta tcacattctt attattatat atttgcgtaa gtagtcctgg
     8701 tagtctcaat gcactattct atcttgaaac taagaagtta tagaaggagc aatgatagaa
     8761 agcacgagaa tacaaatagc gattaaatat ttttctagac aaccttagtt aattcacaca
     8821 gatatatttt aggtacagtc ttacagatat agagttagtg ctgtgtgaat cctgatgttc
     8881 gcagtgcata ggagtcaagt aagattctag ccagtataga aagtggaagt ctggagcgta
     8941 cctcataatc tgggaagaag gagccttctg atttaaagcc aacaaaagga atctcgatat
     9001 gttccatcaa acttgaatga cgattagcag taagcaaaat tgtcccgatc tcagctttat
     9061 aacagttttc agctgcggta gtcaattcct ttgtttcacc gtttagtgaa acaaagatca
     9121 cgatgtcatc tttggataat tttctactaa tgttttttat gatattggga tcagtatgca
     9181 tctcacaata tttaccggta agttgaaatt tgaccatcat ttcctgagca atcaattctg
     9241 aaaaacctcg ggcaaaaatc atgattcttc tagcagcctt gattcgttga atagcatctt
     9301 caatattccc gctttctacc atattgatgg tgcggattac ctcttgctca tttttaagaa
     9361 tagcagtctt tattccttca tcaactttat caagagcggc aaaattgata tttgtatttt
     9421 ctttttcttt taacgaatgt ttaaaagacg taaacccctc atatcctttt ttcttcatag
     9481 tacgaacgac ggttgctgtt gaaacgttgg catattcact tagttttaca atggaatagg
     9541 aggggatttc gagtatgtga ctttgaataa aatcccagag atatttctcc gattcactta
     9601 attttgtatc catattttgc ctccgattag ttaggataat tctattgtaa agcgggagtt
     9661 tcctttactt caagtttatt taatatattt tgtaaaagtt ttcaatgaat ttagtcattt
     9721 atgaaaacat ttacattcga tcttgataca taatgaatat gtaagaaagg aggagcgtaa
     9781 atgtcagttt atacatgtac tttaaacttg gcaatcgacc tttttatcga aacagatgaa
     9841 atgaaaccat cccaagtcaa tcgaacaaac gatgatgata tacaagctaa tggaaaaggt
     9901 gtgaatgttt cattgatcat gaagcaatta ggtatcgata gtacagcttt aggatttaaa
     9961 gcaggtttta ccgggaatta tattgaggat tatttgcatc aaaaagatat tagcactgat
    10021 tttattagcg taccgggaat gactagaatc aacgttttta cacaagttaa taaaaccaat
    10081 gaagaattca aattggttaa taaaggacct gagattcctc aggaacaaat caaaactttt
    10141 ctaaatcaaa tacaaaagct gcaagctgac gattatttgt gtgtttctgg cagtttgccg
    10201 cgaggggtct cacccgaaat tcttattgat atcagtcgta tttgttttga aaataaggtc
    10261 catctgatta ttgattcaag ctatccggaa ataattgatt gtctgccgta tcagccgttc
    10321 ttattaaaac caaacgagga ggaattgcaa tcttggtata atgtcgagct tagtgatcgt
    10381 gaaggatata ttcattatgc tgaacgcttg attgccgatg gagcgaaaaa tgttctcctt
    10441 tctctaggag gagagggagg aatattttta aataaggata tcgtttattc tgggaattct
    10501 cctaaaggta aagtagtcaa tacagcgtgt gctggtgaca ctttgttggg aacatttctt
    10561 gcaggatatt taaaaggtga acaggtagat attaatttga agaaaagttt agcagctgga
    10621 agttcaaccg cttttcgtaa aggattaaca gatttttcgg atgttgcaga gttggaaaaa
    10681 caaatcgaaa tcaaagaggg agcgagtaaa aaatgacaac ttacaaaatt gttgccgcaa
    10741 caggatgtcc aacaggaatt gcccacactt atatggctca agaagcattg gagcaggcag
    10801 ctaagcgcaa aggggtatct atcaaagttg aaacccatgg tcaagtaggg atagagaatg
    10861 aattaacacc agaagaaata aaaaacgctg acgctgtaat cattgcagct gacaaagatg
    10921 ttcaagcaga acgtttcgca ggtaagcgtg taatcgatgt gcctgtcagc aaggggatta
    10981 aagatgctga ccaattgatc gaagatgcgt tggctggtaa aggttcaatc actgcagaaa
    11041 acaaaacagt tgacgctgct ggtgagaaag cggagatggg ggttgcagca ggcattggtc
    11101 acagtattta taaaaatctc atgaacggtg tttctcatat gctgccgttt gtagtggcag
    11161 gtggggtaat tattgcaatt tcgtttgcca tctggggtgt gtactctttt gatcccaatg
    11221 atcctactta caatccgact gccgctatgt taaagggtgt tggtgatgcg gcaatgggaa
    11281 tgatggttcc tattttagca gcttatatcg ctgaaggaat cgctaaacgt cctggtttgg
    11341 ttgttgggtt tgttggcgga ctgttagcca attctggcgg agccggtttc ttaggcggaa
    11401 tttttgcagg tttcttagca ggctacttta tcttactatt gcaaaaggtc atgaaaggct
    11461 tgcctaaaca attggatggt ctgaaggcga tcttcttata tccggtgatc ggtgtcttgt
    11521 ttgttggtgt agtcatgtct ttattagcaa atccaatgga agcagtcaac caaggaatga
    11581 tgaatttctt ggcaggattc caaaattcta atccattaat tttagggatt atcgttggct
    11641 gtatgtcagc atttgatatg ggaggtccag tcaataaagc ggcttatgtt acagggactg
    11701 cgttgttggc acaaggtaac catacattta tggcaggggt ttcggcagct tgtatcgcgc
    11761 ccccattgat tactggattt gccgcactgt tttttggaaa atatttcgac acgaatgatc
    11821 gcaacgctgg tttagtcaac tttatcttgg gctcaacgca cattaccgaa ggagcaattc
    11881 catttgcggc taaagatccg ctgcgtgttc tgccaactat gatgctaggt tcatcaattg
    11941 ctgctgtact gacatatatg tttggtgtcc aagttcccgc acctcatgga ggttttctag
    12001 tattacctgt tgttactggt aaggttgcat gggtagggtc gattttactt ggttcaatcg
    12061 ttggcggagt aattttaggg ttgatccaaa aacgtagtgt agaaaaacgt gaagcagaag
    12121 cagctgctgt tgaaatgaaa gaagcaacca acaactaaag gttattctag aaagaaggat
    12181 ttcaaatgac tttgatcaaa acaaataaag tttttatgaa taaaaatttt acgacgcaag
    12241 atgaggtaat gaacttcctt gcagatcagt caattgtctt gagcattagt gaagaccgag
    12301 atgcagttta cgaaaaattg atggaaagag aagaagaaag cacgactggt atgatggatg
    12361 gttttgccat ccctcatgcc aaagatgcta gtatcaagga tgccgatatt gtgattgttc
    12421 ggttaaacgg taaggtcgat tggaaaagtt tagatggaga aggaacggat tttgtaatcg
    12481 ctctgtttat tccagattca gaggctggaa caacccatct aaaattttta tctcaggtag
    12541 ccagactttt gatgcacaaa gatgtgacag aaggtctgaa acaagcccag tctgctgaag
    12601 aaatagctga acttttaaac caaaaattag aagaaaacta aaaaggaagt agtgaaatca
    12661 tgcctttaac agaaaaaaag aaaacttata ttcgtcaact aagtaatgaa agtggcatca
    12721 tcagtgcgtt agcaatcgat caacgtggtg ccttgaaaaa aatgatcggt aaatttcaaa
    12781 ataaatcagc aaccgatgaa caaatcatca atttcaaaaa aatcatttct tctgaattga
    12841 ctccatatgc ttcagcaatc ttattagatc cagaatatgg gttgccggca gccgaagttc
    12901 gcgataaaaa tgctggtctg ttgttggcct atgaaaaaac tggttacgat gcttcaactc
    12961 caggacgttt accggatatt ttatctattt ggtctgtaaa acgtctaaag gagcaagggg
    13021 ccgatgcttg taagttcttg ttatactacg atgtagatga atctgatgac atcaatgacc
    13081 aaaagaaggc gtttatggag cggattggtt ctgaatgtgt tgctgaagat ttgccatttt
    13141 tcttagaatt ggtttcttat gatgccaacg atcctgatac ttcttcgaag gaatatgcct
    13201 tgaaaaaacc gcataaagta aatgaaatga tgaaggaatt ttctaagcct cgctatggag
    13261 ttgacgtttt gaaagtggaa gttcctgtta atatgaacta tgtggatggt tacactaaaa
    13321 atgaagttgc ttataccaaa gaagaagctg ctgcttattt caaagaacaa tccgagacta
    13381 ccaacctgcc atttattttc ttgagcgcag gagtaactgc tgaattgttc caagaaacct
    13441 tgaaatttgc aaaagaggct ggctctacat tcaatggggt actctgtggg cgcgcaactt
    13501 gggcgaatgg tgtagaacca ttcgttacta aaggtgaata tgctgccaaa gaatggatgc
    13561 agacacaagg acgcaagaac atcgaagagc tgaataatgt tttgaaagaa acagcaacag
    13621 ctattaaatt gtaagaaagc tttaactata accttaagtt tgtaacctaa tgatttttaa
    13681 taatcaattt gagcttttaa ataaagaatt ggaagcgtcc cgacgcttcc tttttcgttt
    13741 gtttttaagg catgataggt tctatgttgt cgttcaataa gcgcaagaag gtcagcattt
    13801 ttttcttaga gtatgcaaaa ttgcttttct acccaatgat ttaaaacgga gaggcaccct
    13861 tggatatggt agatggtgaa gtaacgcatt tcttctgtga tcacggtttt tttgtcttct
    13921 tgtagccaga tttttaaata attttcttcg atcagattat ttaatctctt ttgaaattgg
    13981 atatctccgt ggttgatgac gaataacttg tatacgaacg tattcgtata gacatagtcg
    14041 atcaattgag cgtatagatc ctcgtagcta tgtgtcgtat cgtaggaaat gatttcatcc
    14101 aagtcttgga aaaagctatt ttccagtttt tcataaagat catagatatc cgtataatgt
    14161 gtataaaatg tcgaacggtg aagatcagca gtttcggtca gttcacggat ggtgatgttt
    14221 tgaatttttt tatctactaa taattttgat aatgcttctg ctaacgcttt ttctgttttt
    14281 ttgacgcggc gatcctcttt tttcattttg cagcctccta atcaacataa tcaaggaatt
    14341 gtgtcggtta tccgacaatt tcaccgctat ctgaacatag ataaccgaca ccttgtctat
    14401 tataatataa gtatagcata tcaatattta aagaatagga gaataagtga tggaattagc
    14461 ctttaagcaa ctggaaaaga aagactacaa aaaagccatt caattcgcga ttaaaggtat
    14521 gcactttgat tggtatacca agaacaaact ccttttgaat ctttatggaa gatacttttg
    14581 gtatctagaa ttgaatcgcg cgacacaaat cattgcttta tatgcggatg atgagttggc
    14641 aggtgtttta ttagcagaca tcaaaggaaa atcaaaaaag catcattcat ttagtcaaaa
    14701 gttatatgtt aaactatttg atttcttaca aaatgcattt gtgaaagaca gcaaaggcgg
    14761 gtatgacgac accaatgacg aattgctttc agactattta aaaaaacatt ccccagatgg
    14821 agaaattgtc tttttagctg ccaaccctga tttaaagatc aaggggatcg gtagtaaatt
    14881 attaaaagaa tttgaacgcc gagagcaagg aaaagaagta tttctgttca cggatagcgg
    14941 ctgtacgtat caattttatg aacgacgcgg tttcgaacgg aaaaaagaaa aagataacgt
    15001 catgaagatc cttaataaag aagtagatct ccgctgtatg ttatatagta agatcattca
    15061 gtaatgtttt ttactatcag aaagcagaag taccacgaag gtcaatggaa aaagcgagta
    15121 attgatccat ttagatgaag ataggtgtag tctcaaagat attagctggc aaagatatcc
    15181 ccagttaaaa atagatttct gtatccaaca ataaaaatat ttttgtattt attagtatat
    15241 atactttaca aaaatttgta tatatactaa tttttttgta ggatcaatga aatgagggga
    15301 aaatatgcca aaatataaag aaatagcctt ggctctaaaa gagaggatcg ttgagggaaa
    15361 atatcaggaa ggttctaaat tacctgagca agaaagtctg gctagagagt ttaaaacaag
    15421 ccgtgttact atccgcaaag ctatacaact ccttattgat gaaggtttgt tatatacgag
    15481 acgtggatct ggtactttta tacgaagcaa tatcagacag gacaatgaga atgtcactca
    15541 agtaaataat gtatttggca cttcctcaca agaaaataca aatgtgacaa gtaaaataat
    15601 ccgttttgat gtccggtttc cgacagatta tgaaaggcgg ctttctgcaa cctacttccg
    15661 atccccttcc tgcaaatcat gggtataaaa aataggcata ttaagcgggc taggaggaga
    15721 aatattcaca tatcattttt tcattccgca agctgcgtca agttatttgg aatttgtaga
    15781 acagatgaga gaggtagaag cagcactaca aactactttt caataagtag gaattataga
    15841 aattttactc aaaaaatctt cattaaaaaa tctgactaaa taagttgaga aaacagaata
    15901 tacttcaata tccagtgatt tataaatatc attcactcat gatacccagc tccgtatcat
    15961 aagtgatgcg gtttttatca ttgatcctaa aaatcttagc aagacaaaaa gtacgtaaga
    16021 aagtaaagac taaatacttc ggaggaacaa atgaataaca atgataggtt attgcgattg
    16081 cgttatgcga ttgatttgaa agatacggat ttgattaaag cttttgagct aggcggtgta
    16141 ccactgacta aagaagaagc tcaggcggta ctgaccaaag tacaggataa gaacaaagat
    16201 aatgcagaaa ataatgtgta tgaaaaaacc atcaacaacc aagtttttga cgcattccta
    16261 aatggcctga ttctcttggc gcgtggtccg caaaaagata agccgatagt agacacgccg
    16321 caaaaaagca aggaaatcaa atatatcaac aatgtgttgc taaaaaaatt aaaaatcgcc
    16381 ttgtctatga ccactgatga tattttagaa gtttttgctg aggcagaact ctatccatca
    16441 aaaggcgaaa tcggggcatt tttgagaaaa gaaggccaac gaaattttaa gccttgcgga
    16501 gataaatata tgcgtaattt tctcaagggg ttaggaattt ataatcggag aaaagtataa
    16561 agaaatttca tgagaaatca aaacaatggt tgttttgatg agagctagtt tttgtatgag
    16621 attaaaatat tatcttaatc gaggagctgg attggtttta tgaatgatga atataagaat
    16681 gatgaagata agatgctttt tgaagagata gagaatcgat gtaggctcaa ttttgagctt
    16741 cggggaaaga tgtctttaat acaacaaaaa aagtatttag cgaataaatc agagtttact
    16801 ctgggacatg ttgaaaaatt aatctcggat tggattagta gtagaagcga atttacaaaa
    16861 ataaaacagc cgattaaatt tgacatgaaa aaacttttgt taaataagag tgagataggt
    16921 aatagagatc aatatattag agctaaaggg caagaaataa ttgattcatt aggtgaagtg
    16981 aggtcataca attatttgta tgtaacacat agagctgatg gaatggtcat tactgtgggg
    17041 aaatcttctt ctaatgatat ttttttagat ggagatcttt tttatcaact caatacaaat
    17101 cacttgtctg gcactgaaaa tataatttta aggacggaat atggcaatga aatatttgca
    17161 aaatatgatg agattttaaa aaattatttg gactgggctt ggataattcc ggttgagtca
    17221 ggagatgcta agaaactgga gagattattg ggcgatgaac taattaataa aaaagtacca
    17281 attttaaatt attattccca tagacaataa aaggctattt ttcatgataa tactagttat
    17341 aactgtaaaa atggaagaaa atctgggaaa gaagttttta aataagtgta ttgttcgatt
    17401 ttaataaata ataagcaata ccatccgtct gtgttgcgca acatcgtact acaatgaata
    17461 ttttttcaca aaaagaaaat catccatcga atgaatggaa ggttttagta tacacgaggt
    17521 taatgtcccc tcttttataa tcagaggagt gaggtttttt ctatgaatgt ttctattttc
    17581 ttattgctag tctagtttct cttacgatga aattgggatt ctattccagt ttttattgat
    17641 ttgctgataa aataacttga atggtggata taaacatgat aaataagaga agaataacaa
    17701 aacaaggttg tgaaatgatt ctcaaataag tataatagaa agaaaaagga ggaagaatca
    17761 atgaaaatag caattgcagg cgctggcgca atgggaagtc gctttgggtt aatgcttcac
    17821 caaagtggga acgaagtatt attaatcgat ggctgggcag aacatgtcca acaaatcaaa
    17881 gaacatggat tacaagcaaa ttttaatggt aaagaggtag aagcaaaact accaatcgtt
    17941 cttcaatccg aagtagaaaa agaagatcaa gttgatctga ttattctatt taccaaagcg
    18001 atgcagctgg aaaaaatgct gcaggatatc caatcattaa tcaaaaaaga tacagaggtc
    18061 ttatgtctat taaatggtat cgggcatgaa gatattattg agaaatttgt accaatggaa
    18121 aatatctata ttggaaatac catgtggacg gcaggtcttg aaggtcctgg tcaggtcaaa
    18181 ttatttggaa gtggttcagt agaattacaa aatttaggtg atggaaaaga agcagctgca
    18241 aaaaaattag cagacaaatt gtctgaatca ggattaaatg ctcatttttc tgacaatatt
    18301 cactattcta tttatcgcaa agcatgtgtg aacggaacaa tgaacggact atgcacaatc
    18361 ttagacgtta atatggctga gctaggaaaa acatcgactg ctcataaaat ggtggcgacg
    18421 attgtcaatg agtttgccaa agtagcagca gtagagaaga ttgaactaga tgtcccagaa
    18481 gtcattgcac attgtgaatc ttgttttgac ccggaaacaa ttgggttaca ttatccttca
    18541 atgtatcaag acttgattaa gaaccatcga ttaacagaaa tcgattatat caatggcgca
    18601 atttctagaa aagggaagaa atatggtgtt gcgacgcctt attgtgattt cttgactgag
    18661 cttgtccatg caaaagaaga tagtttgaac gtaaaataaa ggagagattg aatggaaact
    18721 tcacaaaaaa tatcaccgaa aatttttctg aataaagtcc tcgctggtac agcaacagga
    18781 atcattattg gattgattcc taatgcagta ttaggtgcta ttttaaaata ttttagtgcg
    18841 tatccctttg cggtaacgat cgcacagtta gcagttattt ttcaattagc aacacctttg
    18901 atcattggtg gtttgattgc cttacaattt ggatttgatc cgatgagaat gatggttgta
    18961 gccggagctt cttttgttgg ttctggtgta gtcaaattca cacctgattt gggtgaaacg
    19021 ggtgtttacg ttggtgctgg tacaggcgat attattaata cgatgctgac agcatcgatt
    19081 gctgtcgggc tgattttact gattggaaat agttttgggt cagtgtcgat tgtgttaaca
    19141 ccgattgttg taggtgtagg tgcaggttta cttggttttt atttattccc ttttgtaaca
    19201 gcaattactt ctgcaattgg atcattgatt aataacttca ctaccttaca accgctgtta
    19261 atgtctatct tgattggttg ttcctttgcc tttttgatta tctctcctat ttcaacagta
    19321 gcaatcggaa tggcaatcca actagatggt gtttctgctg gtgcggctgc gatgggcgta
    19381 gctgcaacca cttttgtttt agtagtgaac tcttggaagg ttaataagtc aggtgtgaca
    19441 attgcgattg ctttaggcgc tatgaagatg atgatgccta atctatttag aaaacctgtg
    19501 attcttattc cttgtttagt tagtgcagtg attacagcta ttcctgtcgc tctattttct
    19561 atttcaggta cgcctgcttc tgctggtttc ggtgtggtcg gacttgttgg tccgttggct
    19621 tctttagatg cagggctaaa tggctttctt gttgttcttt gttggttagt catccctgtt
    19681 tttgcggcac taattactca atttttatgc gagaaagtat ttaaactgta tgatcgagaa
    19741 gaagtcttca aatttttagg ctagtagatt ttactaagaa attttcaatc aatggatgcg
    19801 taagtgagat gtcaacaaca tctgacttac gtttttttgc caaaatatgg tttttatttt
    19861 ttattattag gtgtgattag tgatttatcg acaccgtata tccttggcct tttttatccc
    19921 aaactgaatc aaatgactac cgtaattagt gtgtttggtg acgtagatag tccagtcaga
    19981 cgtgctttct tggtctggtc agttgtatct ggactttttt ttgttctttc gttgcctgcg
    20041 ttgtatcacc tatttgttgg aacatcaaaa acacttgcaa tacttgctgt tgcaacagtt
    20101 ggtttatatg ggataggtga ttgtattttt actgggttat tcagtatcaa tacgaatgaa
    20161 agctcttgga atctatcgac ttggatccat aatacaggtt ctgggctagg ctatgcaggc
    20221 tttctacttt tcccattgtt acttgttctg ctttatcgtc aaa
//
LOCUS       DACGLW010000049        20220 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.051, whole genome shotgun sequence.
ACCESSION   DACGLW010000049 DACGLW010000000
VERSION     DACGLW010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.051"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            66..1100
                     /locus_tag="IXT53_001811"
     CDS             66..1100
                     /locus_tag="IXT53_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAQ4905618.1"
                     /translation="MQKKISIIITLFFICILASWFIQRQAALYDVPVIEIERVRIEED
                     QQLITGRLMNTDQKGEVISLTAPYQKNQIETANITTRQIYLVQMHGDEWQLVAKKNDG
                     WLFFFTSVFIGTMLLIGGKQGVFALGSLIINIVLLLLLLLLFTKTEGISLLNIAILFV
                     VIGIIVSILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTR
                     PYRSVFLSSVLIGTAGGTLDTVITVIATLEELTKQQPKISAKQLWLSGRQVGRDVIGS
                     MANILLFVYISGAIPSLLLYLGNQWSFKETVERHLSLELLRVIAGSLGIVLSIPIAVL
                     FFLAVRRLKK"
     gene            1097..1864
                     /locus_tag="IXT53_001812"
     CDS             1097..1864
                     /locus_tag="IXT53_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HAQ4905619.1"
                     /translation="MNVLIILVGIFAISVLFVGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILVLSIIGFLLIAVVILFFINGYNLKMRAAFASILIFLFCFLLLIPITDFLAI
                     QGFTSIELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     MSFNQLRHASFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDISFQQIINEKSFVL
                     EYTMAILTTLAALLVLPLTCVTTAYLFTKKKEHLKME"
     gene            1964..3532
                     /gene="liaX"
                     /locus_tag="IXT53_001813"
     CDS             1964..3532
                     /gene="liaX"
                     /locus_tag="IXT53_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HAQ4905620.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDELENGEEKISEPVDPKEKERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEATGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSDDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVNQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATPENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDSIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDDEIAQVQL
                     STTTGSIYLKDTDK"
     gene            3711..4016
                     /locus_tag="IXT53_001814"
     CDS             3711..4016
                     /locus_tag="IXT53_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HAQ4905621.1"
                     /translation="MKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVAF
                     GSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTKKRKEAEKIDDDEWSDF"
     gene            4016..4366
                     /locus_tag="IXT53_001815"
     CDS             4016..4366
                     /locus_tag="IXT53_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HAQ4905622.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALFVAILM
                     SLVNVIVSEHNSRK"
     gene            4532..5470
                     /locus_tag="IXT53_001816"
     CDS             4532..5470
                     /locus_tag="IXT53_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HAQ4905623.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            5485..6315
                     /locus_tag="IXT53_001817"
     CDS             5485..6315
                     /locus_tag="IXT53_001817"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HAQ4905624.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLVLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGSLPDYDRSLGKNQQII
                     "
     gene            6359..7384
                     /locus_tag="IXT53_001818"
     CDS             6359..7384
                     /locus_tag="IXT53_001818"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HAQ4905625.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHHLPDSIRAFTDMEDAVKDADAVLFVIPTKAIRSVAKELVAKLNTKPVI
                     IHASKGLEQGTHKRISEILLEEIPTEKRKAVAVLSGPSHAEEVAKHDITTITAASTNV
                     KAAKYVQELFMNHYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            7399..8337
                     /gene="galU"
                     /locus_tag="IXT53_001819"
     CDS             7399..8337
                     /gene="galU"
                     /locus_tag="IXT53_001819"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAQ4905626.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYIMSLGKTLEAEGSKINHNPKGITEIKEEKKK"
     gene            8462..8911
                     /locus_tag="IXT53_001820"
     CDS             8462..8911
                     /locus_tag="IXT53_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HAQ4905627.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTQDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIVTKVGGIGKTTAKATVAGKVGGTAMKFLKTKNILKRQEENKNG"
     gene            8904..9518
                     /locus_tag="IXT53_001821"
     CDS             8904..9518
                     /locus_tag="IXT53_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ4905628.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSSELTGIAKEKAQDLSQQAGDLAGNVKQKVSESAEEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDLKKPADELGSIAKDTSEDIMIDVKDAADKAGETVNEG
                     MSEAKPVADDTKEMLKMAKEDAKEDAKEAKKEMKDTMTDSTTNH"
     gene            complement(9581..10684)
                     /locus_tag="IXT53_001822"
     CDS             complement(9581..10684)
                     /locus_tag="IXT53_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HAQ4905629.1"
                     /translation="MNNVKINELRQWMAQHQLELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVDRFDAISDILTGTDSYTDLTSIIQRLQLIKTVEEKDKLIEAGKWADVAF
                     EIGFKTVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGSTKVSP
                     NELVLFDLGVIWNGYCSDATRTVAYQKPTEFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLE"
     gene            10894..11895
                     /gene="ccpA"
                     /locus_tag="IXT53_001823"
     CDS             10894..11895
                     /gene="ccpA"
                     /locus_tag="IXT53_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HAQ4905630.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            12249..12644
                     /locus_tag="IXT53_001824"
     CDS             12249..12644
                     /locus_tag="IXT53_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905631.1"
                     /translation="MPKKRRKTIVIILTILTIGIGGKFYMDKREAQKQQDLLAVEKQS
                     VKVLKNTFADIKEVKIEQFEKNDMTGIYEALITMTNTEGKAVYFDYSFTKESNEIDGY
                     GVENEEVQKEGITVDEIIVIYTDGSEEKV"
     gene            12641..13897
                     /locus_tag="IXT53_001825"
     CDS             12641..13897
                     /locus_tag="IXT53_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HAQ4905632.1"
                     /translation="MTITDKQYKGISNKVYWLDPKHPQYSKSYKEGAIKNFGETKFQI
                     LKIKNSFDGMQAMAVAPIKNGKVDISQVVIAFAGTNLSDWKDLETDARSVMNLYGNRP
                     GASFSEKATTNKILSVSGLKTAGQINSAIAFANEVKKEYGEAAISTTGHSLGGFLALY
                     VAAEKQWKNVGFNGPDPYDLLSPEAKKWVEENPGMLTNYRNHADFIGNFGGNGTGAEV
                     RISMETAYLNLLASHSLTSWKFDENGKLIIPENENNVKARKQQKENQATQLFSYQLQE
                     LNNIRKKLRLSGGRLTVNEQIYLDDTQARLVVEKLSVKMKSAMEEVIRINQRGIFELE
                     KKWQEGLRQARVVAPSLTEQEIIEALASVGATKENLVDSAKEVYLLRINKARRMGEAF
                     DTLAKEIQDKINEIVARDRELAQQFN"
     gene            13909..14232
                     /locus_tag="IXT53_001826"
     CDS             13909..14232
                     /locus_tag="IXT53_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905633.1"
                     /translation="MKLEELERDYRSERRQLEAQEDELIHFKNQGLRLMEQEEQRLHH
                     VLKNLALDNEPLFQARRSYSNYQEHYQQALQVERRKLYQQQDELERSYRQKIRQITME
                     KSSYE"
     gene            14225..14491
                     /locus_tag="IXT53_001827"
     CDS             14225..14491
                     /locus_tag="IXT53_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905634.1"
                     /translation="MNEFLMQLLKELEKNLHRSNVRSVGMGISQQTWAEKRLTDQNHQ
                     MTAEEATQEFSNRHAHLKESVKGQFAEKLTELFQRQEKKLKQFR"
     gene            complement(14654..16999)
                     /locus_tag="IXT53_001828"
     CDS             complement(14654..16999)
                     /locus_tag="IXT53_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HAQ4905635.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLLFAA
                     LGIGIGAGYFAYLVEDTQLPTKKELQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIVLSAQVEKHFSKDEIITTYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSEAYYQKAQQTIQNKGYTIHSTIDKDIYAALQTGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLVGSES
                     RVSDYATTWQEGKNAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINEKITTPFKDVISSLNGNLGNA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSQARIRSANYLANLINQAY
                     QANPEIFGTDEKFDLSSDVMKKKVSGFTGQTAGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANPETKESDDKDD"
     gene            17278..18180
                     /locus_tag="IXT53_001829"
     CDS             17278..18180
                     /locus_tag="IXT53_001829"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HAQ4905636.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAFMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGAEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKVIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     ISMFKAQAFDPEVFPELLAEEVTL"
     gene            18391..20166
                     /locus_tag="IXT53_001830"
     CDS             18391..20166
                     /locus_tag="IXT53_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HAQ4905637.1"
                     /translation="MLNILNPLIDQLKKKHTYFILGIAVLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIHYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     ILASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPTVEIIIA
                     CLLAIYYRKYNSSKKLLPWFLGLAVSFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEAKKLKERPFLLSKQEWLKLSLFLLPLVGFSIVYQWIEYKNETTYGIAAIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALDASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYQKGT
                     IKKKKEFYLMGSGDGKTMNDLPIVFDFLKSGYAYAWRYKDFHQGGNYSFGTQEQVEQA
                     QELLNLPLMDNWIDSSDPRTPDFQLTKTAKVANVMIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVIGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIAVTSMLVFSKLNLKKALSSK"
BASE COUNT         6647 a         3604 c         4219 g         5750 t
ORIGIN      
        1 ggaaaagggt ttgtgacaaa acatgtcaca aacccttttc attattccaa tagaaaggat
       61 aaagaatgca aaaaaagatt agcatcatca tcactctatt ttttatttgt atacttgctt
      121 catggtttat tcagcgtcaa gctgctctat atgacgtacc tgtcatcgag atcgaaagag
      181 tgagaataga agaggatcaa caattgatta caggaagatt gatgaatacg gaccaaaaag
      241 gagaagtgat ttctttaacg gctccttatc aaaaaaatca aatcgaaaca gcaaatataa
      301 caacaagaca aatctatctt gtccaaatgc atggagatga atggcaacta gtagcaaaaa
      361 aaaatgatgg ctggcttttt ttctttacaa gtgtcttcat tggcaccatg ttgcttattg
      421 gtggaaaaca gggcgtattt gctttaggaa gtctcatcat caacatcgtt ttacttttac
      481 ttttgttgct cctctttacc aaaacagaag gtatttctct cttaaatata gcaatcttat
      541 ttgtcgttat agggataatc gttagtatat tggcgttaga cggatggaat agaagcagta
      601 taatcaaaat ttcggctgca gttacaagtg tttttttagc attttttatt tgtcttttga
      661 caatgaatca ttggaaagat caaggactgc gctatgaaga actgaattat ctgacacgac
      721 cgtatcgttc tgtttttctt tccagcgtgc tgattgggac agctggaggg acactcgata
      781 ccgtgatcac agtcattgcg acattagaag aattgaccaa gcaacaaccg aaaatatcag
      841 cgaaacagtt atggctttca ggaaggcaag tgggccgaga tgtgattggt tcgatggcta
      901 atatcctttt attcgtctat atcagcggag cgattccatc gcttctgtta tatttaggaa
      961 atcaatggtc tttcaaagaa acggtagaac gacatttatc attagaactt ttacgcgtca
     1021 ttgctggcag cttaggaata gtattatcaa tccccatcgc ggtcctcttt tttctcgctg
     1081 taaggaggtt aaaaaaatga atgtacttat tatacttgtt gggatttttg caatcagtgt
     1141 gctatttgtc ggtggtacgc aaggtatgta tattctttta ggattattca ttaatttagg
     1201 aatctttttc ctattattat tcggctatca tcagaagtgg ccgatattgg tattgtcgat
     1261 tatagggttc ctattgatcg ctgtggttat cttatttttt atcaatggtt ataatctgaa
     1321 aatgcgagcc gcttttgcca gtatactgat ctttttgttt tgtttcctac tattgattcc
     1381 tatcactgat tttttagcga tccaaggctt tacttcgata gaactagaag aactatctgg
     1441 cttagataag acgttagcaa ttgattttag actacttacg aggtcgttgc ttctgatcag
     1501 tcttagcgga gctgttttag atgcgagtgt agcgatttct agtggaacat tcgaagtata
     1561 tcaggcaaat ccgcaaatgt cttttaacca attgcgccat gctagctttg ccattgctaa
     1621 aaaaatattg gcatcaacag tgatgacgct tctttttgcg tttatgggga gttctttagc
     1681 gttgattctt tggtttattg acttagacat ttcttttcag caaataatca atgaaaaatc
     1741 atttgtcttg gaatatacga tggctattct taccacactg gccgcactac ttgtcctgcc
     1801 cttaacttgc gtgactacag catatctctt tacaaaaaag aaagaacatc tcaagatgga
     1861 gtaaaagtta caacttaagt cctatgacat ttttactgaa tcagtgcata atatggatgt
     1921 aaggaaaagc ttgcaaacaa ttttgcttaa ggaggcaatt tccatgaaag aaagagaacg
     1981 tattttagac ttagtgaaaa aaggaatcct ttcgactgaa gaaggcttag atttattaga
     2041 aagcatggca actgaaaaag acgaaaaaca aatcaaaaaa gaagcagaca aggtaactgc
     2101 tagtcataaa gaaaaagatc aggcaagtca attgatcgat gaacttgaaa acggtgaaga
     2161 aaaaatttct gagccagttg atccaaaaga aaaagaaaga caagatcaag aaaacttaga
     2221 aaagatctta gatgaactag caactgaagc gaataaaact tcggcgcgtc tagacgaagt
     2281 aaatgctcaa ttcgctgaca ataaggcagc acgtaatgag aaacaggaag cattgatgca
     2341 gctgaataca aaagaagaat taggcgagtt gacagaagaa gaattagcac agcgtcaaac
     2401 gttagaagca gaaatcaaag agttagaagc aaccggcgat acactattag aagaacaatt
     2461 gaagctggaa gcagaattaa aagatatccg caagaatcaa tggtctgaga aaaaagaaac
     2521 atttacggat aagtttgaat tgccagatga ttggaaagat caagcgacag atacgctgaa
     2581 tcaagtaggc gaaaaaatgt cagaagctgg atcacagtta ggcaaattct tgaagaaaac
     2641 attccaaact gtttcagaaa cagtaaatga taacatggaa tggaaagatg tcagcttacg
     2701 cgtccctgga attgcgacaa cgaaatttga acacgaattt tattatgaag ctcctgcagc
     2761 aagtatcctt gatatcaagg cagcaaacgg aaacgtgacg ttaaaaacgt gggacagtga
     2821 tgatgtgaaa gtggaagcca agatcaaact ttacggaaaa atgggtgctg aaccatttga
     2881 agcattctct gaacggagcc agatcgaggt caatgaagat catatctcct tccaaattcc
     2941 aaataagcgt gtgcgtgcag accttgtttt ctacttgcct aaacgagtat acgaccatgc
     3001 agctatcaaa cttctaaatg gcaacatcat gattgagaca ttggaagcta aagatattta
     3061 tacaaaatct acgaatggga acatcatcgt gaaccaatta actgcaacga tgctggaagt
     3121 agaaggtgta aacggcaata tcgatattcg aaacgggaac atcttagatt ctattataga
     3181 aacagtaaac ggtacagtga catttggtgc cacaccagaa aacctttctg tgtctttagt
     3241 aaatggtgat gtacgtctta cgattaaaga agataatttg aaaaaagtag aagcaagttc
     3301 agtgaacggg aatgtcaaag ttgctttacc agatagcatc ggtttggaag gacacgctaa
     3361 aacaagtctt ggaagcatta acagccgtct gtccaattac gaagtagttc gtgagaaaaa
     3421 agaacggaca aaccaaatgt tgcaattccg tcgtgtttca gatgatgaga tcgcacaagt
     3481 gcagctttca acgacaacag gaagtatcta tttaaaagat acagataaat aaagaggttg
     3541 tgaaagagat gttcagcctt gagcaataag aagcgatttt ttaaaatagc ttcccctatt
     3601 ttaaaaaaat gtgccttatt gccgataggc tatctcttga acaccgatta agaagaaggt
     3661 tgtcacaacg attttcttca tttaaaatag tagaacagag gtgagacatg atgaaaaaaa
     3721 agttgacaaa atccaataaa aatgttgttt tgacaggtac tttagcaggg attggggaat
     3781 atatcgggat tgatcccact gttatccgtg tgctttatgt attcttaagc ttagttgctt
     3841 ttggaagtcc aatcatttta tacattttat tagctttgat tatcccatct gcgcgttctg
     3901 cacgaacaaa ctatggacat aacaatgcat attatgaaaa aaacaattat cgtgaacaaa
     3961 caaaaaaacg caaagaagcg gaaaaaatcg atgatgacga atggagtgac ttttaatgtc
     4021 gtactttcag cgtattttgg ttaatacgtt aacattcata tcgttggctg ttctttttcc
     4081 tgcgatggtc catgtgagca gtatttggac ggccgtcctt gccagcttcg tattatctgt
     4141 gttgaacatg tttgtcaaac cgattttgac gatcctgtcc ctgcccttta cattagtcac
     4201 attgggattg tttacgtttg ttatcaatgc agcgattctt aaaatgactt ccttttttgt
     4261 aggagcagaa cattttagtt tttcttcatt tggtgcagcg ttattcgtag ctatcttgat
     4321 gtcacttgtc aatgtaattg ttagtgaaca taacagcaga aaataaaaaa agctgtgccg
     4381 agatctcggc gcagcttttt tgtttagaat aaagaatgcg ttttctatta cattggtaat
     4441 taaataaaaa cgtttttttt aattggtagt acttttggct agacaatgat aaaatgatag
     4501 agaacactct aatagaaatg aggtagcatg aatgtcagaa gttgtaaaag ttagccaatt
     4561 agtaaaaaaa ttatctttgg aaatcgtcac aggcgacgag aaaagtttag accgcgtcat
     4621 cgtgactggg gacatctcac gcccaggact agagttgacc ggttatttta attattattc
     4681 ccataatcgt ttacagctat ttggcagtaa agaaatcagt tttgcggaac ggatgatccc
     4741 agaagagcgt ttgatcatta tgagaagatt atgtagtgaa gatatgcccg catttattat
     4801 ttcaagagga ttgccggctc cggaagaaat gatccaagca gccaatgaaa atgggatagc
     4861 tgtgctgcgt tcgcctatct caacgtcccg gctgctaggt gaattatcta gttatttaga
     4921 tggacgctta gcccctcgaa caagtgtcca cggtgtgtta gtcgatgtct acggattagg
     4981 tgtactaatt caaggggaca gtgggatagg gaaaagtgaa acggcgttgg aactgattaa
     5041 gcgcggacat cgattgatcg cagacgatcg agtagatgtc tatcagcaag atgaactgac
     5101 ggtaatcgga gaacctccta aaatcctgga acatctgatt gagattcgag ggatcggtat
     5161 catagatgta atgaatcttt tcggtgcgag tgcggtacga ggctctatgc aagtccagtt
     5221 agctgtttat ctagaagcat gggcgaaaga taagaaatac gaccgtctgg gctctgaaga
     5281 cacgaccgtt gaaatcgcgg aagtgggcat tccacaaata aaaatccctg tcaaaacagg
     5341 acgaaacgtt gcaatcatca ttgaagtagc cgcaatgaat ttccgcgcac gtacaatggg
     5401 ctttgacgca acgaaaacat ttgaagaaag attaagtcgc ttgattgaag aaaactcagg
     5461 caatgactag caaggagtga caatatgtta gcacaaatca atcgagttgc ttttgagctt
     5521 gtaggaattc ctatctactg gtatgcattg attatcgtat caggaatcat tattgccatg
     5581 tggctcagca gcagagaagc tgttcgggta ggattaaaag cagatgacgt gacagatttt
     5641 atgttgtggg gattgccgat ttccattatt ggtgcccgct tgtattatgt tttattcgat
     5701 cttccccaat atatagctga tccaatccag atattcaata tccgttcagg aggattagcg
     5761 atttacgggg gattgattgc aggtgcaatc acactgtact tttttaccaa atatcatttt
     5821 atttctttgt ggaccttttt agatatcgct gcaccaagcg tattattggc gcaggcaatc
     5881 ggacgatggg gaaatttcat gaaccacgaa gcgtatggtc cagacacgac acgaagtttc
     5941 ttagaaagtc tgcatttacc gcaattcatc attgataaca tgtatatcga aggaatctat
     6001 cgacaaccaa cctttcttta tgaatcactt tggagtttga ttggatttat tctgcttttg
     6061 gttttgcgga agaaaccaca tcttttgaaa aaaggagaag tccttttatg ttatctaatg
     6121 tggtatagtt tcggacgttt ctttatcgaa gggttacgca cagacagcct ttacttttta
     6181 ggtgtgatcc gcatttctca attattgtct gctgttttat ttgtcggcac attgattctg
     6241 tttatttggc gtagaaaaaa aggaagttta ccggattatg accgctcatt aggaaaaaat
     6301 caacaaatta tttgattttc agagcgttaa aaatgaaaaa aatttcagga gtggtaaaat
     6361 gaattataga gtagcggttt taggaccagg ctcttggggg actgctttag cacaagtcct
     6421 cgctgaaaac ggacatgacg ttcggatttg gggacatcgt gcttcacaag tagaagaaat
     6481 caacacccaa cacaccaatc aacgttattt accagatcat catctacccg attccatccg
     6541 agcattcacc gatatggaag atgcagtaaa agatgcagat gcggtactat tcgttatccc
     6601 gacgaaagcg atacgttctg ttgcaaaaga actagttgcc aaattgaata cgaagccagt
     6661 gatcatccac gcaagtaagg gattagaaca gggaacgcat aaaagaatct ctgaaatctt
     6721 gttagaggaa atcccgacag aaaaaagaaa agctgttgct gttctttctg ggccaagcca
     6781 tgcggaagaa gtagcaaagc atgacattac gacgatcaca gcggcaagca caaatgtaaa
     6841 agcagcaaaa tatgtacaag agctgttcat gaaccattat tttcgaatct ataccaaccc
     6901 tgatgtgatt ggtgtagaga tgggagcagc tttgaaaaat attattgcca ttggagctgg
     6961 tgctattcac ggcttagggt ttggcgataa tgcaaaagcg gcaatcatga cgcgcggatt
     7021 agctgagatc agccgtttag gtgtagcaat gggtgccaac ccgctgacct ttattggctt
     7081 aagcggagta ggtgatctag tcgttacttg cacaagtgtt cattccagaa actggcgcgc
     7141 aggcaacttg ttaggacaag gtcagcctct agaagaggtg ctagccaata tgggaatggt
     7201 tgttgaaggt gtgaatacta ctcaagcagc gatggaacta gcccaaatga tggaaatcga
     7261 aatgcccatc actcaaacaa tctatgaagt gctgtatgaa caaaaagaaa tcaaaacagc
     7321 cgcaaaagaa atcatgttac gtgatgggaa aaaagaaaat gaattctcat taacaatttt
     7381 ttgaaggagc gaatgtatat gaaagtaaaa aaagcagtta ttccagcagc aggattagga
     7441 acaagatttt taccagcaac aaaggcgatg gcaaaggaaa tgttgccaat cgtggacaaa
     7501 ccaacgatcc aattcatcgt tgaagaagcg ttgaaatcag gaatcgaaga catcttgatc
     7561 gttacaggaa aagctaaacg accaatcgaa gatcactttg attcaaatat cgaattagaa
     7621 acaaacctaa gtgaaaaagg aaaaaccgaa ttattgaaat tagtagaaga aacgacagat
     7681 gtcaatttgc atttcatccg tcaatctcat ccaaaggggt taggacacgc cgttcttcaa
     7741 gcaagagcct ttattggaaa tgaaccgttt gtcgttatgc taggcgatga tatcatggaa
     7801 gacgaagtac cattgacaca gcaattgatc gatgactatg agcgtacaca cgcttctacg
     7861 attgccgtta tgcaagttcc tcacgatgaa acttcaaaat acggaatcat caacccaggg
     7921 gaagtactgg aagatgggtt atacaatgtc aaaaactttg ttgaaaaacc agatccaagc
     7981 aaagcaccaa gtgatttagc tattattggg cgttacttat tgactccaga aatctttgat
     8041 gtattatcag aacaacaacc aggcgctgga aatgagatcc aactaactga cgcaatcgat
     8101 acgttgaata aaacacaacg cgtatttgcc cgtaaattca caggtaaacg ttatgacgtt
     8161 ggtgacaaat ttggttttat gaaaacaagt attgaatatg gtctgacaca tccagaagta
     8221 ggggcacctt tgcgtgaata catcatgtct ttaggtaaaa cattagaagc agaaggaagc
     8281 aagatcaatc ataatccaaa aggaataaca gaaatcaaag aagaaaagaa aaaataatct
     8341 ttttttctaa cgagcttcag ctgctaattg aataaaaagt agttgaagct cgtttttatt
     8401 tgtttaaaaa acacgaaaat gctatcctta tagaaaagag agacaagaag ggagtttgat
     8461 catgtcagga ggagaaattg caggattgat cgcagcaatc gcctttgcga tacttgtcgt
     8521 tttcatcgtt cgcgtattgt tgcaagtttc caaaacttta gaaaaagtag accagactgt
     8581 cgcagaagca aatacgacaa ttgagatcgt cacacaagac gtagatcttt tatctagaca
     8641 agtagaaggc ttgctagtca aaagtaatga gctgctcttg gatatcaatg gaaaagtcgc
     8701 aacgatcgat ccattgttca cagcagttgc tgatttaagt gaaagtgtat cggaacttaa
     8761 tcattctagt aaaaacatcg tgacgaaagt cggcggaatc ggaaaaacga cagctaaagc
     8821 gactgttgcc ggaaaagtcg gtggaacagc gatgaaattt ttaaaaacaa aaaacatact
     8881 gaaacgacag gaggaaaata agaatggcta aaaaaggtgg atttttatta ggtgcagtag
     8941 ttggaggtac agccgcagca atcgccgcat tattgttagc acccaaatct ggtaaagaac
     9001 ttcgggatga attggctgct caagcagatg atttgatgga catggcttca gattacacag
     9061 atatggcaaa agaaaaatca tctgagttaa caggtattgc aaaagaaaaa gcgcaagatc
     9121 tttctcaaca agcaggggat ctagcaggca atgttaagca gaaggtaagt gaatcagcag
     9181 aggaagcagg aagcgaatca gatgatatct tgttaaacct caaagaacaa tcagctgatt
     9241 tatctaatcg tttcaaaaaa acagcagaag atctgaaaaa gccagcagac gaattaggta
     9301 gtattgctaa agatacttca gaagatatca tgatcgatgt aaaagatgct gcagataaag
     9361 caggagagac ggtcaatgaa ggaatgtcag aagcaaaacc agttgcagat gacacaaaag
     9421 aaatgttgaa aatggcaaaa gaagatgcaa aagaagatgc aaaagaagct aaaaaagaaa
     9481 tgaaagatac aatgacagat tcgactacca atcactaaga ttactcattt ctagaataaa
     9541 aaaggtgaga gcggaccagc tctcaccttt tttattttca ttactctaaa acaagtaatt
     9601 cttttggtgt agtcgtgaat ggcacacaac catcacttgt cacatacaca caatcttcga
     9661 tacgtacccc tactttttca ggaatataga ttcctggttc aagagagaag cacatccctt
     9721 cttcaatcac taagtcgttt ccttctacaa gagaagggaa ttcgtggacg gttgttccga
     9781 ttccgtgacc taaacggtga ttgaaatatt cgccgtagcc atagctgttg atcacgtttc
     9841 gtgcgatttg atcaagttca cctgctgtca cacctgggcg aactgcctct tgtgcggcaa
     9901 gctgtgcttc aagagtgatg ttatagattt tttcttggaa ttcagttggt ttttgataag
     9961 caactgtacg agtagcatca ctgcaatagc cattccaaat aacgccaaga tcgaataaca
    10021 ctagttcatt tggtgaaacc ttcgtgcttc ctggtgtacc atgagggctt gcagcatttg
    10081 ctcctgcaag gactaatgtg tcgaaagaca tttgtgaaac accttgtttt tttaattggt
    10141 attcgatttc tgcgataatt gcttgttcag ttacgccttc ttttactgtt ttgaagccaa
    10201 tttcaaaggc aacatcggcc catttaccag cttcgatcag tttgtctttt tcttctaccg
    10261 ttttgatcag ttgcagacgc tggataatag aagttagatc ggtgtaagaa tctgtacctg
    10321 tcaagatatc agagatagca tcgaagcgat cgacagtcag agcattcttt tcaataccta
    10381 cattaccagg ttgcccatag cgtgcgcgaa gctcttttcc gatgatctcc caaggatttt
    10441 cgctgtctaa gtagccacgt acatcgtacg cccagctgct gttttgagca tcttcaactt
    10501 ccagagcagg agtgaaaagg aaaggatctt gttccagagg gataaaaaga gctagtacac
    10561 gctcgtgagg atcactttca tatccggaaa agtaagcgat gtggccaggg tcgctgatat
    10621 aagccaattc taattggtgt tgtgccatcc attgacgtag ttcattgatt tttacgttat
    10681 tcatttggca acctactttc taaattaatt ttgtattcaa taattgtacc actgattgat
    10741 taaaagacca acgttcttaa aactgaaaaa agtaaaaatt ttcatgaaaa cagagataaa
    10801 acggttgcaa ttgttgagat tatttgatat cctattttag aaatgtaaga cgtattttca
    10861 gaaaacatat aaaatatgaa taggagaaca caaatggaaa agcaaacaat cactatctat
    10921 gatgtagcga gagaggcaaa tgtttcgatg gctactgttt cacgtgtcgt aaatggcaat
    10981 ccaaacgtca aaccagcaac aagaaaaaaa gtcttggaag ttatcgatcg cttggattat
    11041 cgtccaaatg cagtcgcacg tggcttggca agtaagaaaa caacaacagt tggggtaatc
    11101 attcctgatg tcagcaatat gttctttgcc tctttagcac gtggtatcga cgatgtggca
    11161 acaatgtaca aatacaatat catcttagca aactcagatg gcaacgatca aaaagaagtc
    11221 aatgtattga ataatttatt ggctaagcaa gtggacggcg tgatctttat gggtcaccat
    11281 atcacagacg aaatccgtgg agaattttca cgttcaaaaa cgcctgttgt actagctggt
    11341 tccattgatc cagatgaaca agtaggtagc gtaaacattg attatgtatc agcaacaaaa
    11401 gatgcagtaa ataaattagc aaaaagcggc aataaaaaaa tcgcttttgt cagcggtgcg
    11461 ctgattgatc ctatcaatgg tcaaaaccgt ctaaaaggct ataaagaagc attaagcgaa
    11521 aacaatctac catatagcga aggattgatt tttgaggcac aatacaactt taaagatggt
    11581 ctgtctctag ttgaccggat ccataatagc ggtgcaacag ctgtctatgt ttcagacgat
    11641 gaattagcca ttggtatctt ggatggttta ttagaccgcg gcgtaaaagt accagaagaa
    11701 ttcgaaatca tcacaagcaa caattcttta ctgacagaag tagctcgtcc tcgtttgaca
    11761 agtatcacac aaccattata cgatttagga gcggtatcca tgcgcctatt gacgaaaatg
    11821 atgaataaag aagaagtcga agaaaaaacg attatcttgc cttatggaat cgaagaaaaa
    11881 ggatcaacta aataataaaa gatcaatcat ttaaaggttg ttcccaaagt ggtcaacttc
    11941 tggaacaacc tttatacgag aaaaggggtt atgacaaaat tttgtcataa cccctttttt
    12001 tgtgacttta aaaaaaagag agaatggcgg aaacaaacct tacgtccatc gtatacgaca
    12061 caatctgtgc gtagatgcta cattttgttc actactatca aaattacttt aaaatgaaaa
    12121 cgtgataaaa aagaaagtag aaaagtagaa aagtaactct aaaaaatcca aaaaaatcca
    12181 ggctcgattg cgtacttatc ttaaaggaga aacaatccat catacataac ccgaaaggag
    12241 acttctattt gcctaagaaa agacgaaaga ccatagtcat cattttgacc attcttacta
    12301 taggaatcgg aggaaaattt tacatggaca aaagagaagc gcaaaaacag caagatttac
    12361 tcgcagtaga aaaacagtcc gtcaaagttc tgaaaaatac ctttgcggat atcaaagaag
    12421 tgaaaatcga acaatttgag aaaaacgaca tgacaggaat ctatgaagca ttgataacga
    12481 tgaccaatac agaaggaaaa gccgtttatt ttgattatag ctttacaaaa gaaagcaatg
    12541 agatagatgg ctatggtgtt gaaaatgaag aagtacaaaa agaagggatt acggttgatg
    12601 agattatagt tatctataca gatgggagtg aggaaaaagt atgacaatta ctgataagca
    12661 gtataaagga ataagtaata aagtatattg gctagatcca aaacatcctc aatatagtaa
    12721 aagttacaaa gaaggcgcca ttaaaaactt tggagaaaca aaattccaaa tcctaaaaat
    12781 caaaaactcc ttcgacggca tgcaggcgat ggctgtagcg ccaatcaaga acggtaaagt
    12841 ggatatttct caagtagtga ttgcttttgc aggaacgaac ttaagcgatt ggaaagattt
    12901 ggaaacggat gcaagaagtg ttatgaatct ctatgggaat agaccaggag caagtttttc
    12961 tgaaaaagca acaacaaaca agattttatc cgttagtgga ttgaaaacag cggggcaaat
    13021 caattcagcg attgcatttg ctaatgaagt aaaaaaggaa tatggagaag ctgctatttc
    13081 tactaccggc cattcattag gtggattctt agcattatat gtagcagcag agaaacaatg
    13141 gaaaaatgtc ggcttcaacg ggccggaccc gtatgatctc ttatcaccag aagcaaaaaa
    13201 atgggtggaa gaaaatcccg gtatgctcac caattaccga aatcacgccg attttatcgg
    13261 taattttggt ggtaatggca caggtgctga agttcggatc agtatggaaa cagcatactt
    13321 gaatctgctt gcttcccaca gtttaacctc ttggaagttt gatgaaaatg gaaagctgat
    13381 tattcctgaa aatgaaaaca atgttaaagc tcgaaaacaa cagaaagaaa accaagcgac
    13441 ccagttgttt tcctatcagt tgcaagaact gaacaacata cgtaaaaaac tacgattaag
    13501 tggcggccgg ctgacagtta atgaacaaat ttacttagat gacacacaag ctaggttagt
    13561 ggtggaaaag ttatcggtaa aaatgaaaag cgctatggaa gaagttatcc gcatcaacca
    13621 aagaggaatt tttgagctag agaaaaaatg gcaagaaggc ttgcggcaag cacgtgttgt
    13681 cgccccttcg ttgactgaac aggaaatcat agaagcatta gctagtgtgg gcgccacaaa
    13741 agagaactta gtcgattctg cgaaagaagt ctatctgcta cgaatcaata aggcaagacg
    13801 tatgggggag gcctttgata cattggcgaa ggagatccaa gacaaaatca atgaaatcgt
    13861 ggcacgagat cgagaattag cacagcaatt caattaaagg agaagtagat gaaactagaa
    13921 gaattggaaa gagattatcg aagtgaacga agacaattag aagctcaaga agatgaattg
    13981 atccacttta agaatcaagg attacgattg atggagcaag aggagcagcg actgcatcat
    14041 gtattaaaga acttagcttt agataatgag cctctgtttc aagcaagacg aagttattcg
    14101 aattatcagg agcattatca acaagcccta caagtagaac gacgaaaact ctatcagcag
    14161 caagacgaac tagaacgttc ctatcgtcaa aaaatcagac aaataactat ggagaaatca
    14221 tcttatgaat gaatttttaa tgcaattact aaaagaatta gagaaaaatc tacatcgatc
    14281 aaatgtacgt tcagttggta tggggatatc tcagcaaact tgggcagaaa aacgattgac
    14341 agatcaaaac caccaaatga ctgcagaaga agctactcaa gaattttcta accgacatgc
    14401 acacttaaaa gaatcagtaa aagggcaatt tgcagaaaaa ctaaccgaat tattccaaag
    14461 acaagaaaag aaattaaaac agtttcgtta gtaaagttat atagaaaaat tttgtgaaaa
    14521 tggtttttca catttttaca taaatttgtc ttaatatcgt agatgtaagc ttttgaacac
    14581 catttattcg gaaaaaggga gctgtgacaa gatttttgtc gcagctccct ttaattctga
    14641 ttaatctatt agatcaatca tctttatcgt ctgattcttt ggtctctgga ttcgctctag
    14701 cataagctga agcggtgttc cagtaatctg tatagttttc atcagtaccg ccaacgccga
    14761 atttaaaggc gcttttcttc ggaccatttt ttgcccaaag tgattccact gtttttcctg
    14821 gcgtttggat agatttttta ttgacagtaa cttttccagc tgtttgtcct gtgaacccag
    14881 atactttttt cttcatcaca tcggatgata agtcaaattt ctcatcagtg ccgaaaatct
    14941 cagggtttgc ttggtaagct tgattgatta gattggcaag ataattagcc gaacgtattc
    15001 ttgcttggct gtccattcca gtattgtcat cgtgtcctgc ccaactactg atagtgatag
    15061 atggagttga gacgatcagc catgaatctc ggtattcatt ggtagaacca gtttttccga
    15121 cccaatctgc atttcctaga tttccgttta agcttgagat gacatctttg aatggtgtag
    15181 tgattttttc attgatcaca ctacgcatca tgtcattcat gatcgatgcc gttgcttcgg
    15241 aataaatacg gacagaatta ctttcatgtt tataaatcac attacctgca ttatctgtga
    15301 tggaatcaat gatatagcct tcttcgtaga ctccaccatt tgctaaggcc tggaatccat
    15361 ttgtctgttg aagcgtcgtt acatctactg tccctaaagg agccgattca acgccccaat
    15421 tatcatttgc tgggtagttc atcttcgcca agtagtgctc gtaagcgtat tgtttcgatc
    15481 caccggaatt caacacatct tgatataagt gataagctgg gatattgttg gaccattcca
    15541 gtgactcacg gacggtttgg aaggtgttcg acccttcatt ggtcgcattg acgattttct
    15601 caccagcatt ttttccttct tgccaagtcg ttgcataatc tgatacacgg gattctgatc
    15661 cgacaagtcc catatcgatt gcaggaccgt aaacaagaac cggtttgatg gatgaaccag
    15721 cttgacgttc tgtatcaaag gcgtggttat tttgattctg gctgtaattc cgaccaccca
    15781 cgaagccata gatacgtccg gttctgttat ccatcaatac atttccagtc tccacttggc
    15841 taccagcacc atcatctaac atgtagccat aattttctac gccggtttgc aatgcagcat
    15901 agatatcttt atcaatcgtc gaatggatcg tatatccttt gttttggatc gtttgctgtg
    15961 ctttttgata ataagcttca ctgaccgatg aatctgacat atcagcatta tctttttctg
    16021 ccaattgttg tgcaataatc cttgttgctt cgttcatcac ggtataatac aagaattctc
    16081 tgtcagtctg ttccgcgatt tgttgtggga gaaagtcttt tgtaagatca taggctttcg
    16141 cttcttcgta ttgttctttc gtaatttgtc cttcacggta catactgaat aaaacaaaat
    16201 cttttctggc aagaccggca gaaagatctt cttttagcgc gcctgtattc gtgtatggac
    16261 tatacgtgat agggctttga ggcaaaccag cgatataagc agcttgcggc agagtcacat
    16321 cctttgcatc tacaccaaag attcctctag cagcttcctg gactccagca atattttgcc
    16381 ccttattatt ccgaccaaat ggtgagacat tcaaatacgt agtaatgatc tcatcttttg
    16441 aaaagtgttt ttctacttgt gcagaaagaa cgatctcgtt tgctttacgt ttgaatgttg
    16501 tttcatcggt taggatctgt tgtttgacta attgctgtgt caaagtggat cctccagacg
    16561 agccgatgcc agtcgcttct gaaactagtg ctcgtaaaac agctttaggg acataacctt
    16621 ggtgtttatc gaagtattca tcttctgtac tgataatcgc ggttttcaat aaaggcgaaa
    16681 tcttatcaga tgaaatagtc gtccgcatca gatctgtctg gatttttgat atctctgtat
    16741 tatctgcata aacaatcttt gacgtttccg taatgtttcc tagttctgtc tgcagttctt
    16801 ttttagtcgg caattgtgtg tcttcgacaa gataggcaaa gtagccagca cctattccga
    16861 tacctagagc tgcgaacaac aaaagaagaa cagtaataaa aacagtcaat gattgaaaca
    16921 ctctaaaaat aatcttagga acaaaccatt tttctttttt tcttctattc tgtttttttc
    16981 gagtattttt gcgtggcaaa agggtcacct caactaatgg ttttatttcg ctaattatag
    17041 caaataatag gacaaaaaaa cagacaaggc tgtttttgac aggaatttct ttttcgaatg
    17101 agataaaacg tgaggaaatg atctttttta tgaaagagtt cttaagcttt tctttaggga
    17161 aagtttcagt tactatggag aacagggcaa agaaaaacaa actattttct ttgtacatca
    17221 tcttatcaat atacaggaat tgttcgaaac aattgccttt caaggaggaa tccatccatg
    17281 ttattaggct cacacgtcag catgagcgga aagaaaatgc tgttaggcgc ggcagaagaa
    17341 gcagccagtt acggatcttc gacctttatg atctatacag gcgctcctca aaatactaga
    17401 agaaaagcta tcgaagagat gaatattgat gccggaaaag catttatggc agaaaacgga
    17461 ctaacaaata tcgtcgttca tgcgccttat atcattaatt tgggaaatac gatcaaacca
    17521 gaaatgtttc catttgctgt acaatttctg agagaagaga ttgtccgtgc ggaagcttta
    17581 ggtgctactc aaatcaccct tcacccaggc gctcatgtag gagcaggggc agaagctggg
    17641 attgctagaa ttattgaggg gttgaatgaa gttttgacaa aagaacagac ggcacagatc
    17701 gctttggaga cgatggcagg gaaaggtacg gaaatcggtc gaacatttga ggaattagcg
    17761 caaatcatcg atggagtaaa attgaatgaa aaattatccg ttacttttga tacatgtcat
    17821 acgaatgatg ccggatataa tataaaagaa gatttttcag gtgtaatgga agagtttgat
    17881 aaggtcatcg gattagatcg gcttaaagta ctgcatatca atgattctaa aaatcctcaa
    17941 ggttctcata aagatcggca tgccaatatc ggttttggaa caattggatt tgacacattg
    18001 aacaaaatcg tccatgatcc aatgtttgac aacatttcga aaatattaga aaccccatat
    18061 gtcggtaaag ataaaaagaa agcttacgca ccatatggaa aagaaatttc catgttcaaa
    18121 gcgcaagcat tcgatcccga agtgttccct gagttattag cagaagaagt cacactttaa
    18181 gttttctacc tgacagcaac aaaaaaggat tgggcagtct gccttgtcct tttttgttta
    18241 agaaacaaag gagtgctttt tgcattcgtt ttcgccatgc tccttttgcc ccctcttttt
    18301 aaattcagac aaacagggta tactgataga aaagtaaaaa acgaaaagtt tcacaaacaa
    18361 gtagcaaaac gtgattgaaa ggacgaaaag atgctgaaca ttttaaaccc cttgatcgac
    18421 caactaaaaa agaaacatac atactttatt cttggcatag ccgtattatt tacaattatt
    18481 cggttgtatt tatatagtaa agcaatctat ggaacagata tgtcggcgac ctatgatgat
    18541 cagctgctga ttcactatgc tcaaaatatt ttggatggta aatggctagg ggagtacacg
    18601 actacgacac tttcaaaagg gatttcctac agcttgtttt tagtacttgc acataaactg
    18661 catctttctt atgcagtttt attcggcatg ttcagtattc ttgccagtat attggttgca
    18721 ctagcgttta aaccaatcgt gaagaacaag tatcttcttt taagtatcta tttttatttt
    18781 ttgttttctc cgattagttt tacacatgaa tattccactc gaacatatcg taatgctctc
    18841 gtgatcccga ctgttgagat cattatcgct tgtttactag ctatttatta ccgcaaatat
    18901 aattccagca aaaaattgct gccgtggttt ttagggctag ctgtttcttt tcctttcttt
    18961 tggttcattc gcgaagattc catctggctt cttcctttta ctgtagtcgc gttaggcatc
    19021 agtttcgttc agatcttgct aggacaagac ttttctttca aagaagcaaa gaagttaaaa
    19081 gaacgaccat ttctcctttc gaaacaagaa tggctgaaac tttcattgtt tttactccct
    19141 ctagtcggtt tctccatcgt ctatcagtgg atcgaatata aaaacgagac aacatatggt
    19201 attgcagcca tcaatgaccg ttcgagtggc gaatttggac gtttgatgaa acagctgatc
    19261 cgaatagatg acgaaacaaa cttaaataaa gaaaacagtg agatatgggt ctcgcatgct
    19321 gcattaaata aagctttaga cgcatctcct acatttcata ctgtttctac tcgtattgac
    19381 gaattgtata cgactcatgc gtggacccaa ggcggaaaag taaaagaatt accaggagat
    19441 atcatttttt gggcattgcg agatgtggta agcgaatctg gttattacca aaacaatgca
    19501 aaagaaacca atgatttttg gaaaaaagtc aatcaagaat tagaagaagc ctatcaaaag
    19561 ggaactatca aaaagaaaaa agaattctat ctgatgggat caggggatgg aaagacgatg
    19621 aatgatctgc caatcgtatt tgattttctg aaatcaggat atgcttatgc atggcgatac
    19681 aaagatttcc atcaaggggg gaattatagt tttgggacgc aagaacaagt cgaacaggca
    19741 caagaattat tgaatcttcc actaatggat aattggatcg atagcagtga tccgagaaca
    19801 cctgatttcc agttgacaaa aacagcaaaa gtcgcgaatg tcatgataaa aatttaccaa
    19861 gttctggcca ttcccgtgct gattgcttca atagcaggga tattacttat gatactagga
    19921 accgtatttg caaaagctga tcgtgcctat tatagttcct ctttgattgt agtcatcgga
    19981 ttaatactga cacaattggt ttttcttttc ggtgtctcgt ggttttgttc ttatgcgcca
    20041 gaaaaaaagg attatttttt aagtgtctac actggcgcag gcgtgccatt aacgcaattc
    20101 atcgcagtaa caagcatgct agtttttagt aaattgaatc tgaaaaaagc tttatcttcc
    20161 aaataaaaag ggaactgtga caaaagtctt gtcgcagttc ctttttccga ataaacggtg
//
LOCUS       DACGLW010000050        20205 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.052, whole genome shotgun sequence.
ACCESSION   DACGLW010000050 DACGLW010000000
VERSION     DACGLW010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20205
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.052"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            42..197
                     /locus_tag="IXT53_001831"
     CDS             42..197
                     /locus_tag="IXT53_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002392227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905638.1"
                     /translation="MVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGVKDVLLNL
                     FNRIVGA"
     gene            210..713
                     /locus_tag="IXT53_001832"
     CDS             210..713
                     /locus_tag="IXT53_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAQ4905639.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKEQIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCNLAEELEGTPIGEAASEIQHAFFNSFEEMVEH
                     VDDIVYYPDCDDMSDVAHYLIVEAGDFGEVPEQLKPYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPE"
     gene            840..1877
                     /locus_tag="IXT53_001833"
     CDS             840..1877
                     /locus_tag="IXT53_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905640.1"
                     /translation="MANIQTSFIDFHNSIRLDVEDNTLLKDYKDQVIDGLKDYLPDDV
                     KFETFLQGSYSVYTGIKSCDEKIDFDIDIAVAFEIDHTVYEDPREPKLWVKEALVEIF
                     PNAQVNLKVPCVTATFTGKKTKKNVHVDVAVYAKEDENYFLAKAKEFSAPENRCWEEA
                     DPKVLKEKINSHVADSDDRKQFRRCIRYLKRWKDNNFNQEYKPTGIGLTINVMDTFLV
                     NKSTDFLTRKVQYNDMECMKQIVSSLKDSFVYEYSETDGWHYRLHAYLPVKPNSDTYS
                     KMTVNQMSDFKNKLSKLYDDLIFAIDTEDEYEATKRLNNQFGEDFLIISEEEVTEKNL
                     RNAFVTDYPSA"
     gene            1892..3589
                     /locus_tag="IXT53_001834"
     CDS             1892..3589
                     /locus_tag="IXT53_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine biosynthesis protein ThiF"
                     /protein_id="HAQ4905641.1"
                     /translation="MDYTDILKEKLTDVVRLFDGNYYGDFSFATIGKLRLKIEIPKHF
                     PLVLPRIYIHNFDHLGLYLPHVEKDGLVCFSSDINYLVDEENSELVLLQCVNKTIKTI
                     EEGLKGLNKNDFRKEFVAFWDKQENQISLFYLCEPALFIKSVDTIMYGKSLVALDKEN
                     ELQLNAASRLFNGEKEDTHELDAYYVPIRNKNNIMPPKNNKAWSKKDILQIVKKNVTG
                     ANKRFFLKLEKRKNRHNVLLILDIPIEEKSNNVIIGFLFTKQSGSIKDYKGTPRTVLV
                     QRHDELYLVNRTSGENYGLGANIAIVGVGSVGSKIAENLGPLGVRKMTLIDNDCFQAE
                     NLYRNSLGADSLKNARSQNNFFKVDLMQEELEKKIPYLCVEVESMNVLDLISMQPGFF
                     KEYDYVFVCIGDTMASLALNNYFSKSSQKVFYSWLEPYGIGGHTLFINYSLNQKGCFN
                     CLYTDSNSKNRCSNRASLVKEGQIFERTLASCRSKFIPYNAIASTECAINAIKLFHKV
                     NQNNMTNSTLFSWIGDGSTFENLGYQYSDRNTFLNGVNKFEERFFSKYCKVCGELDD"
     gene            3582..4043
                     /locus_tag="IXT53_001835"
     CDS             3582..4043
                     /locus_tag="IXT53_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905642.1"
                     /translation="MIKFSCDRIVIEVRDEVLKIFEQYRQGKDDLPEAGGVLHGKRLK
                     NGSIIITDITTPQIGDIQKRLLFKKNREIHQQLSNEKWIESNKFSIALGEWHTHPEAV
                     PTPSNVDKKSWRLNVSKQHDDRVYVFIIVGFSDLRMWLLSKKSDIRQAIKL"
     gene            4056..4916
                     /locus_tag="IXT53_001836"
     CDS             4056..4916
                     /locus_tag="IXT53_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905643.1"
                     /translation="MKSQKNIWWWGFLVGKVMIPALLLISLPMITFFFTNKKMSKEAV
                     IFFFGDQKATFIETLVTSLQLNLNYIFSIIIVISLLNFFKKRNSGKVFNSNGNVYYNY
                     FYFVFWVAATLLGYDKIQIAGIPIHMQYKLVLSGIFSEVLPDIYDDHYDSDGTCKVSI
                     EKENFDDIDGYDSVNLLIIDTYDIKMSELSMENQTYPTIIVRGNSIDGVRKVNRSLIL
                     EIKKTMDEIQKSDFKKVFVASTSNPKNSINIINSSFRFFGRSRRFKLYVLQKDYASNG
                     KYSKKYRIFI"
     gene            4954..5343
                     /locus_tag="IXT53_001837"
     CDS             4954..5343
                     /locus_tag="IXT53_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ4905644.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLMFV
                     IVFAHVPPLSMIEGAFLKYLGIPIAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            5330..7777
                     /locus_tag="IXT53_001838"
     CDS             5330..7777
                     /locus_tag="IXT53_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ4905645.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDLQTDALVSMI
                     GDSQIDYRFFIGFKLISTDEEVNLKNLRKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVKRVTPSDLTYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPTRCLIEEKPRYLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAKSVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HKEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALASAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVIVELRKEDSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDKEDESNQKCMRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            7782..9890
                     /locus_tag="IXT53_001839"
     CDS             7782..9890
                     /locus_tag="IXT53_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HAQ4905646.1"
                     /translation="MKKKLFRIFGIVLISLVTLIILLSLVGTVAEATGLVDDTVGAGN
                     LYSQYSLGNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEVGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMNMFNLQSNDSQSMGRRVMRKPQMLMNRTLR
                     QLNRNVGRTLAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEIPSDLNKEK
                     PIPNNKKQSRMNLAGRKVGKVLDTQALVKDKTKQVKDGVRNTPTNLKYNFHKGIEKTK
                     KAPEEFKRGLVQEKANRGELREKQQQRRDEKIAEKRKVLNEIGNPHGKRRENVPIKAK
                     VQSQKDRAPKRIVRANSNPEIKRKFASQEIISKGINQSSSKKNSFQQVKRRSTLSKRT
                     NRKVQKSLNPHPKPRSGEKK"
     gene            9887..10891
                     /locus_tag="IXT53_001840"
     CDS             9887..10891
                     /locus_tag="IXT53_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAQ4905647.1"
                     /translation="MNVKKLSAISLTFLTISFMGILMCVGLLFGEDSEGSSSSGAPPG
                     GSSVSEEVLKHRAMVEKYAKESDITEYVPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNFGGGYLDYVAKNGKKHSFALA
                     ESFSKEKSCGQKIDYPNPIANKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSRPETGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTESYWQQHLIGAGRYK
                     "
     gene            10910..11821
                     /locus_tag="IXT53_001841"
     CDS             10910..11821
                     /locus_tag="IXT53_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ4905648.1"
                     /translation="MKIKIERNQKEKSLKKKKERVVSVGKHRKMVLALWLLLSCSLTF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGIENFTKDFSKEYFSWKNNKEAIEKRMNNL
                     EQYLTEEGFALSQDMVRADIPTNSEVQSVKVLDVEKSSEEFVVSFLVEQKITEGKKAQ
                     SISSAYRVTIFEDENRNYIVTSLPTMIAKPDRAKYKTKQVENDSKIDAKTTEEIAEFL
                     ETFFKLYPSASEKELEYYVENDAMRPINIDLKFSKLEDPVYLQNGKKILVSTKIKYLD
                     NVSKTTSYFQYKLILQKNGNWKIIDVK"
     gene            12008..12295
                     /locus_tag="IXT53_001842"
                     /pseudo
     CDS             12008..12295
                     /locus_tag="IXT53_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(12483..12698)
                     /locus_tag="IXT53_001843"
     CDS             complement(12483..12698)
                     /locus_tag="IXT53_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905649.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(12957..13124)
                     /locus_tag="IXT53_001844"
     CDS             complement(12957..13124)
                     /locus_tag="IXT53_001844"
                     /inference="COORDINATES: protein motif:HMM:NF018933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ4905650.1"
                     /translation="MGSECGHTDGKKSFTIREWTCPICRTHHDRNVNVSINILIKGYV
                     YKHCLRKHQEP"
     gene            complement(13469..14908)
                     /locus_tag="IXT53_001845"
                     /pseudo
     CDS             complement(13469..14908)
                     /locus_tag="IXT53_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172772.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(15456..16127)
                     /locus_tag="IXT53_001846"
     CDS             complement(15456..16127)
                     /locus_tag="IXT53_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HAQ4905651.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRYLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCQCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEKWIAYITLK
                     KRGTI"
     gene            complement(16476..17927)
                     /locus_tag="IXT53_001847"
     CDS             complement(16476..17927)
                     /locus_tag="IXT53_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HAQ4905652.1"
                     /translation="MKKSLKLASAVILMSQLFIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTLSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTTSN
                     AESSTTSSSESSTISSAESSTTSSSTSEITSSKVTRPNHIESSKKEAPKTEGQTKNFS
                     TPQQIESTESFQSQPILSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIA
                     QAILESASGQSQLAQAPNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYEN
                     YEESFEDYAKLLTKGISGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNEL
                     IKTYDLTNYDKEVKGPQMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIH
                     AVKAGKVVKAEFHYSWGNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGST
                     GNSTGPHLHLEICKDSSLSQSMLVDPKTLLFGD"
     gene            complement(18039..19382)
                     /locus_tag="IXT53_001848"
     CDS             complement(18039..19382)
                     /locus_tag="IXT53_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HAQ4905653.1"
                     /translation="MKKIVIIGSNHSGLAAARFLNLCDSNYEVILIDKKSEISYLECG
                     TSLFIKREVSNLEDLFYQNRRELNSKIKKLFLETAVDKIDIQNKKIICVKKDGTRFSQ
                     EYDKLILATGSKQIMLDVPGNNLANIFSIKNFPNAKEIFKALDDNTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGKKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHIDNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPVFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
     gene            complement(19516..>20205)
                     /locus_tag="IXT53_001849"
     CDS             complement(19516..>20205)
                     /locus_tag="IXT53_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ4905654.1"
                     /translation="NPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTA
                     GNKKGIVVVTYPDGSKEEVEVTISVEDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTV
                     TLPNGSQYHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNEKDSE
                     KAVSKDNKTDNQGSKQNKNRGKSSPQKQSSKAYPKTGEIDSNIFTISGGLILLGTLGL
                     LGYKNRKKENE"
BASE COUNT         6516 a         3424 c         3759 g         6506 t
ORIGIN      
        1 aatggttttt aaccaatgct caatctttag tcttactagc gattgtggtc attggtttgt
       61 ttttaggatt taaacgggag ttttccaaac tgattggctt cttggtgatt gctttgattg
      121 cggtaggtct ggtctttaat gcttctggtg tgaaggatgt cctactcaat ttgtttaacc
      181 gaattgtcgg tgcctgaggg ggaatcacaa tggaacagat gcgtgtttat attgcgaacc
      241 ttggaaaata caatgaaggt gagttggtgg gggcatggtt taccccacct gtcgattttg
      301 atgaagtgaa agaacagatt ggattaaatg acgaatatga agagtacgcg attcatgatt
      361 atgaactgcc ttttgagatt gatgagtata cgccaattga agagattaat cggttgtgta
      421 acttagcgga ggaactagaa ggaacgccaa ttggtgaagc tgcttctgag attcaacacg
      481 ctttcttcaa ttctttcgag gaaatggtag agcatgtgga tgatattgtc tattatccag
      541 attgcgatga tatgagtgat gtggcgcatt acttgattgt agaagctggt gattttggcg
      601 aagttccaga acaattgaag ccgtatattg attttgatgc ttgcggtcga gatttggaga
      661 ttggaggaaa ttatttggtt actagtcgtg gagtttttga gtatccagaa tgaatcaaac
      721 actatatata gtgtttgatt agtatatgta agcaaaaaaa cacaatatat agagtgcaaa
      781 ctgaagggta atgtgatata atatttgccg aagaaatatt tttgaaatga ggtaatatta
      841 tggcaaatat tcaaacaagt tttattgatt tccataattc aattagattg gacgtagaag
      901 acaatacgct acttaaggac tataaagacc aagtaattga tggattaaaa gattacttac
      961 ctgatgatgt gaagtttgaa acttttttac agggaagcta ttctgtttat accggaataa
     1021 aaagctgtga tgaaaaaatt gattttgaca tcgatattgc agttgctttc gaaattgatc
     1081 atactgttta tgaagatcca agggagccta aactgtgggt aaaagaggcg ctagttgaaa
     1141 tttttcctaa tgctcaagtt aatttaaaag taccttgtgt aactgctaca tttactggaa
     1201 aaaagacaaa aaaaaatgtg cacgttgatg ttgcggttta tgctaaagaa gatgaaaatt
     1261 atttcttagc aaaagcaaaa gaattcagtg ctcccgaaaa ccgatgctgg gaggaagcgg
     1321 atcccaaagt attaaaagag aaaattaata gtcatgtagc agattcagat gatcgaaaac
     1381 aatttcgaag atgtattcgc tatttaaaaa gatggaaaga taacaatttt aaccaagaat
     1441 ataaaccaac aggaattgga cttacaatta atgtgatgga tacgttccta gttaataagt
     1501 ccactgattt tttaactaga aaagttcagt ataatgatat ggaatgtatg aagcagattg
     1561 tttcttcgct gaaagattca tttgtgtatg agtacagtga aacagatggt tggcattatc
     1621 gtctacatgc atatcttcca gtgaagccta acagtgacac ctattcaaaa atgacagtga
     1681 atcagatgag tgattttaaa aacaaacttt ctaaattata tgatgatttg attttcgcta
     1741 tcgatactga agatgagtat gaagctacaa aacgattaaa taatcaattt ggggaggatt
     1801 ttttaattat ttcagaagaa gaagttacag aaaaaaactt aagaaatgca tttgttacag
     1861 actatccgag tgcttaggtt ggtgttaaag attggattat actgatattt taaaagaaaa
     1921 gctaacagat gttgtgcgtt tattcgatgg taattactat ggggattttt catttgctac
     1981 aattggaaag ttgcgtttaa aaattgaaat accaaaacat tttccattgg tacttcctag
     2041 gatttacatt cataattttg atcatttagg actttaccta ccacatgtag aaaaagatgg
     2101 gttggtatgt ttttcttcag atataaatta tttagtagat gaagaaaatt cagaattggt
     2161 tttattgcag tgtgttaata aaactataaa aacaattgag gaaggtttga aagggttaaa
     2221 taaaaatgat tttcgaaaag agtttgttgc tttttgggac aaacaagaga atcaaatttc
     2281 gttattctac ctttgtgaac cagcgttgtt tatcaagtca gttgatacaa taatgtacgg
     2341 taagtctttg gttgctttgg ataaagagaa cgaattacaa ttaaatgcag cgagtagatt
     2401 gtttaatgga gagaaagaag atactcatga attagatgca tactatgttc caattagaaa
     2461 caagaataat attatgcccc cgaagaataa taaagcatgg tcaaagaagg atattttaca
     2521 aatagtaaaa aaaaatgtaa caggtgcaaa taaaagattt tttttaaaac ttgaaaaaag
     2581 aaagaataga cataatgttt tacttattct agatatccca attgaagaaa agtcaaataa
     2641 tgtcataatt ggatttcttt ttacaaaaca atctggatct ataaaagatt ataagggtac
     2701 gcctaggact gtattagttc agcgtcatga tgaattatat ttggtgaata gaacgagcgg
     2761 agaaaactat ggattgggtg ctaatatagc tattgttggg gtagggtcag ttggcagtaa
     2821 aattgctgaa aatcttggtc cattaggtgt gagaaagatg acactcatag acaatgattg
     2881 ttttcaagct gaaaatttgt atcgaaattc acttggtgca gatagtttaa aaaatgcccg
     2941 atctcagaat aattttttta aagttgattt aatgcaggag gagctcgaaa aaaaaatccc
     3001 atacctttgt gttgaagtgg agtcaatgaa cgttttagac ttaattagta tgcaacctgg
     3061 ttttttcaag gaatatgact atgtttttgt ttgcatagga gatacaatgg caagcctggc
     3121 tttaaataat tatttttcca aaagtagtca aaaagttttc tattcttggc tggagccata
     3181 tggcattggg ggacacactc tatttataaa ttattctcta aatcaaaaag gttgtttcaa
     3241 ttgtctttat acagattcaa actctaaaaa taggtgttca aatagagctt ctctggtaaa
     3301 agaaggacaa atttttgaaa ggactttagc gagttgccgc tcaaaattta taccatataa
     3361 tgctattgcc tcgacggaat gtgcaattaa tgcaataaaa cttttccata aagtcaatca
     3421 aaataatatg accaatagta ctcttttttc atggatagga gatgggagta cttttgagaa
     3481 tttaggatat caatatagcg acagaaatac ttttcttaat ggagtcaata agtttgaaga
     3541 aagattcttt tctaaatatt gcaaagtatg tggggagtta gatgattaaa ttttcgtgtg
     3601 accgtattgt cattgaagta agggatgaag ttcttaagat atttgaacaa tatcggcagg
     3661 gaaaggatga cttgcctgaa gctggaggag ttcttcatgg aaaaagactt aagaatggga
     3721 gcataataat taccgatata acaacacctc aaataggaga tatacaaaaa agattactct
     3781 ttaaaaaaaa ccgagaaatt catcaacagc tttctaatga gaaatggata gaatcaaata
     3841 agttttctat tgcattggga gaatggcata ctcatcctga agctgtacca actccatcaa
     3901 atgttgataa gaaaagctgg agactgaacg tgagcaagca acatgatgat agagtttatg
     3961 tttttattat cgttggattt tctgatttga gaatgtggtt actgtcaaaa aaatcagata
     4021 ttaggcaggc tataaaacta taaaggagtg attaggtgaa atcacaaaag aatatttggt
     4081 ggtgggggtt tttagtagga aaggtaatga taccagccct tctcttaatt tctctaccga
     4141 tgattacttt tttctttacc aataaaaaaa tgagtaaaga agccgtgatt tttttctttg
     4201 gagaccaaaa agctaccttt attgaaacgt tagtaacatc tcttcagctt aatttgaatt
     4261 acattttttc aattattatt gttatatcgt tattgaattt ttttaaaaaa agaaattcag
     4321 gaaaggtgtt taactctaat ggaaatgtgt actacaacta tttttatttt gtattttggg
     4381 tagcagcaac tttacttggt tatgataaaa ttcagattgc aggaattcct atacacatgc
     4441 aatataagtt ggttttgagt ggaatttttt ctgaggtgtt gccagatata tacgatgatc
     4501 attacgacag tgatggaaca tgtaaagtaa gcattgaaaa agaaaatttc gatgacatcg
     4561 atggatatga ctctgtgaac ttactgatta ttgatactta cgatataaaa atgagtgaat
     4621 tatctatgga aaatcaaaca tatcctacga taattgttag agggaattca atagatggag
     4681 ttcgtaaagt taatagatct ttaatcttgg aaataaaaaa aacaatggat gaaattcaaa
     4741 aaagtgactt taaaaaagta tttgttgctt caacatctaa tccaaaaaat agtatcaata
     4801 ttattaattc atcgtttaga tttttcggtc gatcacgtag gtttaaactt tatgttcttc
     4861 aaaaggatta tgcaagcaat ggaaagtata gtaagaaata tagaattttt atttaagaag
     4921 gaatttttcc ttctttttat tttggaggaa actatgaaaa aaataaaaag ctacaccagt
     4981 atttgggcag ttgaaaaagt catttatgcg attaatgact ttcagctgcc ttttcctgtg
     5041 acctttaacc agatggcttg gtttgtttta tccttgatgt ttgtcattgt atttgcccat
     5101 gtgcctccgc tatccatgat agagggggct tttttaaaat acttgggcat tccgatagcg
     5161 gtcacttggt tcatgtcgca aaagaccttt gacggtaaga aaccattggg atttttacga
     5221 tcctttatca gctatcactt acggtttaag gtcacttttg caggaaagaa agttaaagaa
     5281 cagaagaaac gatgggacga accaatcacc ttggtaagga gtgtgaatta tgtacccgat
     5341 taagtatatt gaaaataatc ttgtctttaa ccaagaaggc gagtgttttg cttactatga
     5401 attagtgcct tacaattact cctttttgtc acctgaacag aagtatcaag tacatgataa
     5461 ctttcgccaa ttaattgcgc aaaatcgtga aggtaagatc catgccttac aaattgccac
     5521 agaaagtagt attcgtgcca cacaagaacg ttcgaaaaag gaaattaccg gtagactcaa
     5581 agaagtagca aagcaacgaa ttgatttaca gacagatgca ttagtatcca tgattggcga
     5641 tagtcagatt gattatcgct tctttattgg ctttaaactg atttcgacag atgaagaggt
     5701 caatctaaag aatctcagga aatctttctt ttctggactt caagaatttg tctatggagt
     5761 gaatcatcat ttgatgggtg attttgtttc cttatccaat gaagaaattc gtcgctatac
     5821 caagcttgaa aaactaatgg aaagtaaatt ggctcgaaga tttaaagtca aacgagtaac
     5881 gccgagtgac ttgacgtatt taattgaaca tatctatggt gaaaaaggca caccgtttga
     5941 agaatatgaa ttccagctac caaagaaaaa gctgaaatca gaaacgctgg tgaaacgtta
     6001 cgacttatta cgtcccactc gttgtttgat tgaagaaaag cctcggtact tacggatgga
     6061 acatgaaaat catgagtctt atgtagcgta cttaacaatt aataccattg taggggaaat
     6121 ggagtttcct tcctcggaac ttttctatta ccagcaacaa caatttacgt ttccgattga
     6181 tacttcaatg aatgtggaaa ttgtgacgaa taaaaaggcc ctagctaccg ttcgtaataa
     6241 gaaaaaggaa ttaaaagatt tagacaacca tgcctatcaa tctgataatg aaacgaactc
     6301 caatgtatta gatgctttgg attccgtgga tgaattggaa accaccttgg atcagtcgaa
     6361 agaatccatg tataaactaa gctatgtggt tcgagtgagt gctaaatcag tggatgaatt
     6421 aaaacgccgc tgtgatgaag tgctcgattt ttatgacgat accaatgtaa aactagtccg
     6481 gccctttggc gatatgatgg gtctacacaa agaattctta ccctcaagta agcgatacat
     6541 gaatgactat attcaatatg ttacgtctga ctttttggcg ggacttggtt ttggggctac
     6601 tcaaatgcta ggagaattag aaggcattta ctttggctat aacgtagata cggggcgcaa
     6661 tgtttacctg aaaccggcat tggcatcaca aggggtcaaa ggctcagtaa ccaatgcttt
     6721 ggcatctgct ttccttggtt cgttaggtgg ggggaaatct tttagcaaca atctcttagt
     6781 ctattatgcc gtcctttttg gtgggcaagc tgtgattgtc gatccaaaag gagaaagagg
     6841 cggttggaaa gaaacgttgc cagaaattgc ggatgaaatt aatattctta acttgaccag
     6901 taagccagaa aatcaaggct tacttgatcc ctatgtgatt atgaagaaaa agaaagactc
     6961 agaaagtttg gcgattgata tcctgacctt tttaacaggg atttccagtc gggatgggga
     7021 gaagttccct gtgttacgca aagccattcg aacggttacc caaagtgatc agcgaggatt
     7081 attacaagta atcgttgaat tgcgtaaaga ggactcacca gtcgcggaaa atatcgcgga
     7141 tcacatcgag tcctttaccg attatgattt tgcccatttg cttttctcag atggttcgat
     7201 tacccagtcg attagtttag ataagcagtt aaatatcatt caagtagcgg atttggtttt
     7261 accagatgca gaaaccactt ttgaagaata taccaccatg gaattgctga gtgtttccat
     7321 gctaattgtt atttccactt ttgccttgga ctttatccat agcaaccgag aaattttcaa
     7381 gattgtggac ctggatgagg cttggagttt tcttcaagta gctcaaggaa aaacgttatc
     7441 gaataaatta gtgcgtgctg gtcgggccat gaatgccggt gtttacttcg tcacgcaaaa
     7501 cagtgatgac ttgttggatg aacgtttgaa gaataatatt gggctaaagt ttgcatttcg
     7561 cagtaccgat attcatgaaa tcaagaaaac cttggaattc tttggattag ataaagaaga
     7621 tgaaagtaat caaaaatgca tgcgagattt ggaaaatggg caatgcttga tgcaggattt
     7681 gtatggtcga gtaggtgtgg tccaagttca tccagtcttt gaagaacttt tccatgcttt
     7741 tgatacccgt ccgccagttc aagagagaat ggagtgagga gatgaaaaag aaactttttc
     7801 gtatctttgg aattgtttta atcagtttgg tcactctgat aatacttttg agcttagttg
     7861 gaacggtagc tgaggcaaca ggtctggttg atgatacagt tggagccgga aatctttatt
     7921 cccaatattc acttggtaac tatcagttgg atttctttgt ggatagttcg tgggattggc
     7981 tgccttggaa ttggggtgat ggcctaggga agagcgtaat gtatggactt tatgccatta
     8041 ctaattttat ttggacagtt agtctctact tatcaaatgc cacgggctac gtggttcaag
     8101 aagcctataa gctggatttc atttcagata cggctgaaag tattgggaag aatatccaaa
     8161 ccttagccgg cattaccgaa aatggactcc agacctcggg cttttacttt gggtttctat
     8221 tattgatgat tttagctcta ggagtttatg tggcgtatac aggattatta aaacgagaaa
     8281 ctaccaaagc agtccgggct gtcattaatt ttgtgatgat ttttctactt tcgggttcgt
     8341 tcattgcgta tgcgcccacc tatatcacaa aaatcaatga ttttagctca gatgtcagcg
     8401 aagcggctct tagccttgga accgagattg tcgtgccgaa ttcagaaagc caaggaaagg
     8461 acagtgtgga tttaatccga gatagcttgt tttccattca agtccaacaa ccgtggttac
     8521 tcttgcaatt tgatgattcc aatatagaag aagttggcga agatcgtgtt aacaaaatcc
     8581 tgtctgtaag tccagatgaa aataaaggca aggatcggga agaagcggtc aaagcggaga
     8641 ttgaagataa tgacaatgcg aatctcagta ttaccaagac catgaaccgc ttagggaccg
     8701 tggtcttttt ggtgttgttc aatattggca tatccttctt tgtctttctc ctaacaggga
     8761 ttatgttgtt ctcgcaaatt ctctttatta tctttgcgat gtttttgccg attagtttct
     8821 tattgtccat gttgccgact tatgagagtt tgggcaagaa ggcgattatt cgcttgttca
     8881 atacgattat gatgcgagca ggagttactt tggtgattac gactgcattt agcatctcca
     8941 cgatgttttt caatatttcg gcaacctatc catttttcat ggttgctttt ctgcaaattg
     9001 tgacctttgc cggtatctat ttcaaattgg gcgacattat gaatatgttc aatctccaga
     9061 gtaatgatag ccaatcaatg ggaagacggg tcatgcgaaa accgcaaatg ttaatgaatc
     9121 gaacattgcg gcaactcaat cgaaatgtgg gacggacttt ggcttttggt ggtggtgcag
     9181 cagttggaaa taagttggca aaggaacaat caaaatccaa atttaagcca tccggttctt
     9241 ctctacgaaa gaattcacga ttaccgaacg atcacgagat accatctgac ttgaataagg
     9301 aaaagccaat tccaaataat aagaaacaat cccgaatgaa tttggccggg agaaaagtcg
     9361 gcaaggttct ggatacccaa gctttggtga aagataagac caaacaagta aaggatggag
     9421 tacggaatac gccaaccaac ttgaaataca acttccataa aggaattgaa aaaacgaaaa
     9481 aagcaccgga agagtttaag cgaggactgg tccaagaaaa agcaaatcga ggagagttgc
     9541 gagaaaaaca acaacaacgt agagacgaaa aaatagctga aaagcgcaaa gtattgaacg
     9601 aaatagggaa tcctcatggg aaaagaaggg aaaatgttcc gataaaagca aaggtccaat
     9661 ctcaaaagga tagagcacca aagcggattg tgagagcaaa ttcgaatccc gaaatcaaac
     9721 gaaaattcgc tagccaagaa atcatttcaa aaggaataaa ccaatcctca agtaagaaaa
     9781 attcttttca acaagtgaaa cggcggtcta ctctgtccaa acgaaccaat cgaaaagtac
     9841 aaaaaagttt gaaccctcat cccaaaccaa ggtcaggtga gaaaaaatga acgtaaagaa
     9901 attaagtgcc atttcgttaa ctttcctaac aatctctttt atgggtatct tgatgtgtgt
     9961 gggcttattg tttggtgaag acagtgaagg aagtagtagt tcaggtgctc ctcctggggg
    10021 gagtagtgtc tcagaagagg ttttgaaaca ccgtgcaatg gtggaaaaat atgccaaaga
    10081 atcggatata acagagtatg ttccgattct tttagcgatt attcaagtgg aatctggtgg
    10141 cacaatggaa gatgtgatgc aaagttctga gtcagccggt ttaccaccga attcattaag
    10201 taccgaggca tccatcaaac aaggatgtct ctattttgcg tctttggtta agcgttccaa
    10261 agaattaggt tgtgatcaag atagtatcat tcaggcctat aactttggcg gtggttattt
    10321 ggactatgtg gcaaaaaacg ggaagaagca tagttttgct ttagctgaat ctttttcaaa
    10381 ggaaaaatct tgcggtcaaa aaatcgatta ccctaatccc atcgcgaata aggaaaatgg
    10441 tggatggcgc tataactatg gcaatatgtt ttattgctta ttggtgaagc aatacctaac
    10501 tacaacacag tttgacgata aaacagtcca agggattttt gacgaggcgt ttaagtatga
    10561 aggaacggct tatgtatttg gtgggtcaag acccgaaaca ggatttgatt gtagtggctt
    10621 gactcaatgg tgttatgcca aagttggtct gaaacttcct cgggtggctc aagatcagta
    10681 tgatgccatg acccatattg acctaaaaga tgccaaacca ggggatttgg ttttctttca
    10741 ttcgacttat gatgccggta cttatgtgac ccatgtcggt atttatgcag gggagaatcg
    10801 catgtttcat gcaggagatc cagtaggctg gacgaatcta acagaaagct attggcagca
    10861 gcatcttatc ggtgctggac ggtacaaata agagaaaaga ggaaaaagaa tgaagataaa
    10921 aattgaacgg aatcaaaagg aaaagagcct aaaaaagaaa aaagaacgag tcgtatccgt
    10981 gggtaagcat cgaaaaatgg tgcttgccct ttggctttta ttatcttgta gtcttacttt
    11041 tggcgtttat aaaaatttca ccgccattga tcaacataca actcatgaaa aagtggttat
    11101 taaagagaaa gtagtgaata cttctggtat tgaaaacttt acgaaggatt tttcgaaaga
    11161 atatttttcg tggaaaaata acaaagaagc aattgaaaag agaatgaaca acctcgaaca
    11221 gtaccttaca gaagaaggtt tcgctttaag tcaagatatg gttcgagcag atattcccac
    11281 taattccgaa gttcagtcag taaaagtctt agatgttgaa aagagctcgg aggaatttgt
    11341 cgtttcattt ttagtggagc aaaagattac agagggaaaa aaggcacaat cgatttcttc
    11401 tgcataccga gtgacgatct ttgaagatga aaacaggaat tatatagtaa cgagtttgcc
    11461 aactatgatt gcaaaacctg atagggcaaa atacaagaca aaacaagtag aaaatgattc
    11521 gaaaattgat gccaaaacta cagaagaaat cgctgagttt ttggaaacgt tctttaaatt
    11581 atatccatca gcttctgaaa aagaattgga gtattatgtt gaaaatgatg cgatgcgacc
    11641 aatcaatatt gacttaaagt tttcgaagtt agaggatcca gtttacctac aaaacggaaa
    11701 aaaaattcta gtgagtacaa agataaaata ccttgataat gtatcaaaaa ccactagcta
    11761 ctttcagtac aaattaatat tacaaaaaaa tggaaactgg aaaatcattg atgttaaata
    11821 ataatgactc aatctaaatt tatgagaagt tataagtagt ttaacttatt tcaggcagaa
    11881 gtcgcccatc ctctataggt gggtgatgaa tgccgttcgg taactcgaaa accgacaatt
    11941 aagacatagt gcctttttat tttaatccat gtcataatca gtctatataa gaaatggttg
    12001 atataccatg gaattagata caaataatca ttcattattt cttctttgtt accaccttat
    12061 tttagtagtg aaacattgta aacatctcat tgatgatgta atgttagctt atgcaaaaaa
    12121 ctatctttga aagaatatca gaatcacatc acattacctt agttgagtga aatcgtgata
    12181 aagactactt tcatattatg ctcaaagcac aacctaaaac cgaattaaca aaattcagca
    12241 atgcctaagc tcatgtctga taaaatgaga ctttctaagg gtcaaaaatt tcctttgaat
    12301 acgatcatta gcttagataa aagtaaaaac cttagaacta cggggatagc ttggttaata
    12361 agagatatcg ctgttaataa agacataagc ttgcaagtat gctctattcc caagaatctc
    12421 ccacttcaag cactaaggat cgttaaattc agctaagtgg caagtagttc actagacgat
    12481 aattatctcc atatttcaat ctcagctttt ccatttttca tagcaaattc cgttattttt
    12541 atagcatcta aaagattcag cttttccact gatctttcac cacttttcag cttttttatt
    12601 gtgcttaaat tgataccagt accttttgat attgaattgg aagtaaaatc attaagggtt
    12661 ttttttatta aattcatatc cgcaattcca gcgttcataa gaatgaagat tctcctctca
    12721 tagactactt tgaaaatggt actacagtgg ctgtttatat atttcagtag gttactgtag
    12781 tggtcatttt attataatta gaaaaggaac ctaaatcaaa aatgctaaag aaaattttca
    12841 tagtattaaa cctatcatga ctaaagccac ggaattcttg ataaaagagt atacttgcaa
    12901 gcctatgtct ttactaacag cgatatctct tattaaccaa tctatccccg ttgttcctaa
    12961 ggttcttgat gttttctaag gcaatgcttg tatacatagc ccttgatcaa aatattgata
    13021 ctaacgttga cgtttcgatc atgatgtgta cggcaaatag gacaagtcca ttctcgaatt
    13081 gtgaatgact tcttaccatc tgtgtgtcca cattctgaac caatttgact agacggaaat
    13141 catttatcta tcttgatgat ttttttgaca gaagtatcga aaatctttac ataatctggg
    13201 tgttgcattc ttatgcagat gagagcgtta gctccgtatt cataaccagt tgcctctaaa
    13261 acgtagtcca tcggtggata cctagcttgc ggttgccagt ggctttcgca gtcttcttgt
    13321 aatcaaatcg agcatctaat tgagtactag tattttgaag ataatacttg agtttttcgg
    13381 ttttcagaag ttcacttcat cttccatcat ccatggaaat cccactcacc catacgcagg
    13441 cctccaaaaa ttcttcaaca atggtttttc agacattttt ctgaatacct ccttgttttt
    13501 tgatacgcca gatagtcagg aaaaaggcaa caaaacacaa aacaaagctg cctaaaaagg
    13561 taatcttcat tccatataaa aagacatccg gccgatctgc gatatagtta gttactcgtt
    13621 ggcccatctt agcagacatc gcttgatata agaccgtcgt agaaagcgaa atcccgatga
    13681 ccatccccaa atttcgggca aaggaattca tggagcctgc cagtcccaaa ttttcgatag
    13741 tgacagaact cataaccatc gtattatttg gggactggaa caaggcattg ccggcaccca
    13801 tgatggcagt agcgacaatg tacagccaca aaggagagtc cactgacaaa aacatataca
    13861 tgacttgggt taccgacaat aaacccaagc ctactaatac aaaaatctcc gggccaaccc
    13921 ggtcggtcag gtatccgctt aaaggcgccc cgacgaccat catcaaggga aagaccatca
    13981 tcaacagtcc ggcatagcta ggactcaacc cgcgagcatt ttgcagataa aaaggaatca
    14041 ctacattaac aaagaagttg gaagaaaaaa tcaacaaggc tgtaatcaag ctcatggtaa
    14101 aaatcttgtt cttaaaaata ccgaaagaaa tcaatggaga ggctgctttc ttttcaactt
    14161 ttaaaaagct gacaaaggca atcactgaaa ttacgaacag tacaaggggg actggatgga
    14221 aaaagccact ttcttgcccc aaaaagattg ttccaaaaag gctgataatc gtagccgcaa
    14281 tggcagcaaa gccccctcca tcgattgtat ggccgctcat ggtgatatct tttggtaaga
    14341 atttttcagc aatcaacatc gttacgattc ccaccggtac attgatccaa aaaatgtacg
    14401 cccaagaaaa ttgggacaaa atcaagccgc caagacctgg tccagcaatc gagcctaatg
    14461 aaacaagcgc accgattgct cccaatgccc gaccacgttc atgtatggga aagacttccg
    14521 tgatgattcc ggcatttgta gccatagtca tactagcacc aatggcttgt accacccgtg
    14581 cgaacaatta aatacctagc gaatgattga acccacacaa cagcgagccc aagacaaaga
    14641 tcagcgtacc aatccgaaat actttgattt tcccacagct atcgccaatt ttaccaaaca
    14701 gcaacaaaca agaacagaca atcatcagat aaacggacac tacccattcc gcttgattca
    14761 ttggtatgcc catatcatct gagatggtag gtagtgcaat attgacaata ctgccatcca
    14821 atgtggacat gaaggtaaac atagatatag caatcaaaat ccaccagcga tttttttgga
    14881 cttttgcatc ttcttgataa ctatgcataa taggatttcc tttctaatga tcgtgtagct
    14941 aagaacaata taacctatgt ctactgccca acaacttctc gcgactctag ccataggaag
    15001 cttgagtaat ttcgatttgt tattctcatg taaccatgta agtttgagat gctttatttg
    15061 aacttactct atagtaacta gtgataattc gctttacttt cttctagcaa tttctaaaag
    15121 tcgacttcat tgttcttcag attaatagtg tgagctatct cgtgagacgt aagaaaattt
    15181 ccattctact ttttcccaat tttttcatat ttatggtgtt gcacctattt gttttccaat
    15241 ttgcttttca aatacattgc tatgatattc aattccatag gattatacta aatgtcaata
    15301 gtatgaataa tatattatag atgtttatat atcactgtta attattatcc acaataatta
    15361 atgattacct cgatatttgt caatcaaagg ttgaaacaat ttattttcag catcttttct
    15421 aacttgagct gcactcttaa tagaatcata cgatcctaaa tagtacctct tttttttaaa
    15481 gttatatacg caatccattt ctctttagaa cgataaactc ccttaacacc acttgtatta
    15541 tttttcggta attttgacgt tagacactct attactgtcc cttcaacaca tgtccttgtt
    15601 tcttgccttt ttaaacatcc acaactttga gtatgtccaa aaaataagtt actataactt
    15661 atctcacaca tactgccaca ctcacattga caaacccatt ttgttttcca tgaacagtta
    15721 ttgtccaaag gatactttgc agtaagtaat ccgaattttt tattttcaat gtgatgcttt
    15781 tttgatttca aacatccaca gctttttaca tcacctttta gaagtcgctt tgccgatact
    15841 aacttttttc tcccacacag acattgacat tcccaaacaa cagttccttc acttcgcagt
    15901 ttagttggct tgattgcaac taacttacca aaagttttat ttgtcaaatt tttatattta
    15961 tgcctatttt tataatttcc acatccacaa cttttggttc tacctgatag taagtttgat
    16021 cgatatacaa tcgtttcatt cccacagtca cataggtaac gatatttttt atatccatta
    16081 tgagtaatct cctcaacaac agtaaggtga ccatatcttt ccttcactgc acctaccctc
    16141 ccaagaaaga agtaccatat aatttcaatt aacttttaat aaatcagaaa aaaatatttt
    16201 caatttttct tagtctgatt ttatgactaa tttataatac aagactgaac gatacgtaca
    16261 cgaaatcctc aagatagata aattgttttc atctagtaaa acctgctcaa aatatgagca
    16321 taaagacggc aataatacac tcgatattcg ataatggact tgttttattt gtcaaactca
    16381 tcgtaatcga gatatcaatt ccagtctcaa tatcctgatc aaaggtctaa gaatccaacc
    16441 attttcttgt tactttgtca gattgattac cttttttagt ctccgaataa taaggttttc
    16501 ggatcaacta acatgctctg agaaagacta ctatctttgc aaatttccaa atgaaggtgt
    16561 gggccagtac tattcccagt acttcctaca tagccaatta tttgtccttg caaaacatta
    16621 tctccagttt ttactaggta ttgttgctgg tgagcatata acgcggttga tccatcttcg
    16681 tgttgtatca ccacgtaatt tccccaagag tagtgaaact ctgctttaac aaccttacca
    16741 gctttaactg catgaatcgg ctcaccctgt gtagctgcca tatcaagtcc tcggtgaaat
    16801 tcatttccac gatttccaaa caaactggtt attgaataat tatgaatagg cacttggtat
    16861 ccagtcttac tcatttgtgg acctttaact tctttatcat agttggttaa atcataggtt
    16921 ttaattaatt catttagctt caaatagtat tgtgtatctg ttgcataccg tcctgtaagg
    16981 aatttagttg cttctcgata tgttttacta ttagctttta atgctcctgc ataaaaatct
    17041 ttatttccac taattccctt agttaataat ttggcataat cttcaaaact ttcctcatag
    17101 ttttcataca ctctaaattt tgattgggtc gtataaagcg ttccattacc caaatcttcg
    17161 tttgttacca taatgacaaa gtttccatta taagttcctt ttatcccaaa taaattgtaa
    17221 ttcggtgcct gagctaactg gctttgacca ctggcagatt ctaagatcgc ttgtgcaatc
    17281 ataacagaag catataaatc atatttctga ccaatttttc tcgcagactc accaattttc
    17341 ataataaaat tttctacaga ttcatttttg tcaaaatgaa tttcttcatt accagatagt
    17401 attggttgtg attggaaaga ttctgttgat tctatttgct gtggtgtcga aaaatttttt
    17461 gtttgtcctt ccgtttttgg cgcttccttt tttgaagact ctatatggtt tggtcttgtc
    17521 actttcgaag aagttatttc acttgtacta cttgaagttg ttgaagattc ggcacttgaa
    17581 atcgtactac tttcacttga cgaagttgtt gaagactcgg catttgaagt cgtactactt
    17641 tcacttgacg aagttgttga agactcggca tttgaagtcg tactactttc acttgatgaa
    17701 gttgttgaag actcagcatt tgaagtcgta ctactttcac ttgataaagt tgtcaaaagt
    17761 tcactactct ctgctgttac atcggaatta ctcatttcag tcgtctcggt tctttctttt
    17821 gcaattttgt atgttgaatt ttcgcttgcc aacacatgga aaggtgttgc tataaagagt
    17881 tgggacatta atataactgc tgatgcaagt tttaaagatt ttttcatttg atttacctct
    17941 cttttttctc tttaatagtg ttttctatta aaaattatga taaatatttt caatgaattg
    18001 agtaatacag tatatttctt gatttcattt agtaacgatt atgtttcaat gtttaatcct
    18061 gttactgcct ttaaaataaa attatagggt tggctaaaac taggatgaaa taaataatct
    18121 aacattttca atttgtctaa tgtaatttgt tcttctatag caagagaaaa cattgataat
    18181 agtccagata aatcttcttg actacaaagt tgtacaccta ctattctctt tgtatttttt
    18241 tcaaagctaa ttcttaattt tacacgacta ttttttctca aaaaaacagg tctaatgaaa
    18301 tcttctattt ctatataatc tatagactca cttaataatc gtgctgtttt taaattcaaa
    18361 cctgctgcgt aaaaattgta attaaatata gaaaagccat ttgtaaattg ggaaccagaa
    18421 ggtgtaaaat cttgattgtc aatatgttga gttgcaatat aagctgttct tagagcatta
    18481 gaaatagaaa aatcaacact acttctatta atggacgtaa aaaaagttgt agcacaatcc
    18541 ccaatagcat atatatcttt tatatttgtt tgctggtatt cattagttag atatgctcca
    18601 ctaacatctc tcaagagttc ctttttgcca attggactat tagatagaaa tcctatagct
    18661 tgtacgacca tatccataga gtattgggta ccacttgtag tgactatttt ttcaatcttt
    18721 tttcttccta aatatttttt aggactttct gatagacaaa gatgtattcc attttttgat
    18781 agtatttctt caactttatc tgaaaaacta cggtcgtaat aagtcgacaa cactctatct
    18841 gcaacatcga aaagatatac ttctttccct ctttttctta tggcttcagc aatttcaact
    18901 ccaatatatc ctgctccaat aattcctact tttttaatag tattatcatc taaagcttta
    18961 aaaatctctt tagcatttgg aaaattcttt atcgaaaaaa tattagctaa attattcccc
    19021 ggtacatcta acataatttg ctttgaacct gtagctagta taagtttgtc atattcttgg
    19081 gaaaatcttg tgccatcttt ttttacacaa ataatctttt tattttgaat atcaattttg
    19141 tcaactgctg tctccaaaaa caactttttt atcttagaat ttagttctct tctattttga
    19201 taaaaaagat cttctaaatt gctaacttct ctttttatga acaacgatgt cccacattct
    19261 aagtaactaa tttctgattt tttatctatc agaataactt cataatttga atcacataag
    19321 tttagaaatc gagctgcagc tagccccgaa tggtttgatc caataattac tatttttttc
    19381 acttaatcac attcttttcc cttctatgaa aagtatcttc atttttaact aacaacctat
    19441 ctcccaaaat aaaaaacaaa aagaggaaga aaaagattct tcctcttttc agagaagatt
    19501 taagaaattg attttctatt cattctcttt ttttcgattt ttatacccta ataaccctaa
    19561 agttcctaac aggattaaac caccactaat agtaaagata ttactatcaa tctctcccgt
    19621 cttaggataa gcttttgagg actgcttttg aggactgctt ttgcctctat ttttgttttg
    19681 cttgctacct tgattgtcag tcttattatc tttactaacg gctttttcac tgtctttttc
    19741 gtttgaagaa acaactacat tagtaggttc actagtatta ccagattcat cagttgcagt
    19801 tactatcact tttgttcctg cctctaattt aggaacttta actgtaaaat taccattttt
    19861 atcagctgtc ccatggtatt gactcccgtt tggtaatgta actgtcacct ctgcatttgg
    19921 ttcagtttta ccagtaacaa tttggtcacc ctctgtaata ggatcaactt gaggtttatt
    19981 cggtgctttt ttatcttcta cagaaatagt aacctctact tcttctttag atccatctgg
    20041 atatgttact actacaatcc cttttttgtt tccagctgta ctaacatcta ctttctcttt
    20101 ccaagtataa gtagttcctt ttggtagatc ttctttgttc ttaattccct cagatgcgtc
    20161 tggctcttta tttaagtctg tagtcacttt ttgtccttca ggatt
//
LOCUS       DACGLW010000051        20126 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.053, whole genome shotgun sequence.
ACCESSION   DACGLW010000051 DACGLW010000000
VERSION     DACGLW010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20126
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.053"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            261..1130
                     /locus_tag="IXT53_001850"
     CDS             261..1130
                     /locus_tag="IXT53_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HAQ4905655.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            1339..2364
                     /locus_tag="IXT53_001851"
     CDS             1339..2364
                     /locus_tag="IXT53_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HAQ4905656.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            2403..3353
                     /gene="manA"
                     /locus_tag="IXT53_001852"
     CDS             2403..3353
                     /gene="manA"
                     /locus_tag="IXT53_001852"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HAQ4905657.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            3447..5150
                     /locus_tag="IXT53_001853"
     CDS             3447..5150
                     /locus_tag="IXT53_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ4905658.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            5152..7185
                     /locus_tag="IXT53_001854"
     CDS             5152..7185
                     /locus_tag="IXT53_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HAQ4905659.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            7196..7636
                     /locus_tag="IXT53_001855"
     CDS             7196..7636
                     /locus_tag="IXT53_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ4905660.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(7836..8963)
                     /locus_tag="IXT53_001856"
     CDS             complement(7836..8963)
                     /locus_tag="IXT53_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ4905661.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8966..9643)
                     /locus_tag="IXT53_001857"
     CDS             complement(8966..9643)
                     /locus_tag="IXT53_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4905662.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9907..11673
                     /locus_tag="IXT53_001858"
     CDS             9907..11673
                     /locus_tag="IXT53_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HAQ4905663.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            11688..12125
                     /locus_tag="IXT53_001859"
     CDS             11688..12125
                     /locus_tag="IXT53_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ4905664.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            12138..13289
                     /locus_tag="IXT53_001860"
     CDS             12138..13289
                     /locus_tag="IXT53_001860"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HAQ4905665.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(13351..14049)
                     /locus_tag="IXT53_001861"
     CDS             complement(13351..14049)
                     /locus_tag="IXT53_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobyric acid synthase"
                     /protein_id="HAQ4905666.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(14042..15394)
                     /locus_tag="IXT53_001862"
     CDS             complement(14042..15394)
                     /locus_tag="IXT53_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HAQ4905667.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(15617..17017)
                     /locus_tag="IXT53_001863"
     CDS             complement(15617..17017)
                     /locus_tag="IXT53_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HAQ4905668.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            17178..17915
                     /locus_tag="IXT53_001864"
     CDS             17178..17915
                     /locus_tag="IXT53_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HAQ4905669.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            18040..18519
                     /locus_tag="IXT53_001865"
     CDS             18040..18519
                     /locus_tag="IXT53_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HAQ4905670.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            18516..19283
                     /locus_tag="IXT53_001866"
     CDS             18516..19283
                     /locus_tag="IXT53_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905671.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            19513..20082
                     /gene="lepB"
                     /locus_tag="IXT53_001867"
     CDS             19513..20082
                     /gene="lepB"
                     /locus_tag="IXT53_001867"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ4905672.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
BASE COUNT         6833 a         3603 c         4016 g         5674 t
ORIGIN      
        1 tgttcaaaag cttgcatctg cggtaataag cccaaccaaa cgaaaaatag gaggagctat
       61 ttttgtttgg ctgttcttat tacttgatgc agaacagctt tttcacaacc tctttttata
      121 agagttttta ctcattaatt tacaaaaaac aagcaaaatg cgaattttct cacatttttt
      181 ttaacaaaat caaagttatt gcttgaaaga agaagaatcc atcgttaaaa taaagcttga
      241 cgtagagggg agcaagaaat atgaaagatt ttcaattggg ggctgccacc tatttaaaaa
      301 gtgtagccat tcgggtgaaa gaccgagata aaatgattga tttttacaag aacgttgttg
      361 gctttgattt gaaaagagaa gaaaacgaat tagccatttt aggaacagaa gaagctggaa
      421 gtgaaatgtt gctgctggaa gaaagtccta gagcgaatga tcacatgggg gtgatcaaaa
      481 agctgaaccg tttttcattg attatcccta gcacagaaga aatggcagat atcctttgtc
      541 gtatccgtaa aatgaattat ccaatcaaag aagctttaga agatcatgga cgtatgggga
      601 ttttgctcgt agacccagaa gagaatgaat tggaaattta ccatgaagag aataagaacg
      661 accaaagaga aattccagaa acaatggatc aagaagcatt attaagaaaa tctgaagaaa
      721 aattcagtaa actatcaaca ggttcttatt ttgaaaaaat ccatttaaat gttactaact
      781 taacaaaaga acgccaattt cttaatcatg tacttggttt gaaggtacat aatgaagaag
      841 agggcatcca tgtattgaat gaaggagatt tccatgttgg tttgacagaa gctagcggag
      901 gcgcaattga tttgcctaca gatgaagtga ttggacttga ttttctgaaa ttcatgatcg
      961 atactgaaag catggaagga ctgattgctc acttgcagca actatcagtt gatttcttct
     1021 tagacaaaaa gcacacagta ctcacaatct atgatgctat aggaatcgaa tggtggttcg
     1081 tcagtgagcc aaaagaaaag aataaaactg acattcgatg cgacgcttaa ctttttcttt
     1141 cttaacgatt gtcgcatgtc tcgtcatttt atgaacggtc atactatcgc ttattctaga
     1201 gatagaaaga gaaaatagta taccggggga aactgcgcgg tttttcgcgc agtttttttg
     1261 tgtaaaaaag gttgattcct catagattaa gctttaagct tggactgtaa gaatctgata
     1321 gaaaagcagg agaaaaagat gtatccacac acgaaacagc ttattttgaa aaagatggaa
     1381 gaaggatttt atccaggtgt agcagtcgcc ttcataaaag aaaatcaagt agaaacattt
     1441 acagccggct ttgcccaagt cgttcctgaa aagcagctga tgacagaaga tctgttgttt
     1501 gatgcagcct cactgacgaa agtcgttgca accacttcat tagttttacg tctattagaa
     1561 gatggaaaga tagaactcga tcaaccgctt cataaatatc ttcctgaatt tgaaaatcag
     1621 acaatcactt tgcgtcattt actgacacat acttctgata tcaaaacatg gataccaaac
     1681 agagataagc tatctcagga agaattgatc gccgcttatt tgaagctaga accaggtgag
     1741 caactaggaa aagaagtaaa atatacagat gccggtacga ttcttcttgg gttgatgcta
     1801 gaggaaattt accaagaaac tgcagtggaa atctttcaag aacaagtatt ggaaccatta
     1861 ggaatgatga acagcttatt tcttcctttt ccttccaaaa aaatcgttcc cacagaacaa
     1921 ctgccttcgg gtgaggtttt gcgtggacaa actcatgatc cgaaagcaag agttttggct
     1981 gaacatgcag gtaacgcggg tctctttatt gatttgaaag acagcattac ttttgtccaa
     2041 atgtatttga acaagggaaa aatccgtaat ggtcgttttt tagaagaaga gacgattgat
     2101 tctttactgc aagatcaaac accgaaaaaa aatggctatc gttctttagg atgggactta
     2161 aaaaaggatg tagaagacgg tcatgcgatt ctttttcata ctggttatac aggtacgttt
     2221 ttcttgattg atataaaaac acaagaagcg ttgatatttt tgtcaaatcg cgtacatcct
     2281 gtcgatcatc gtgcggagta tctcatatgt cgagatgaaa ttgttgaaaa ctttttgaaa
     2341 gagaaagcgg tattaccgaa ataatgctat aatagctatg aaaaatgaga ggagtttgaa
     2401 ttatgcagga acctatgttt ttaaaaccag tttttcaaga aaaaatttgg ggaggaagtc
     2461 gtttacgttc agtctttggt tttgatatcc ctaacgataa aatcggagaa gattgggcga
     2521 tcagtgcgca tcctcatggt gtcagtgtag tagaaaatgg cgaattcaaa gggaaaagat
     2581 tagatgaact atggtcagaa cacaaggaat tgtttggcca tccttcagaa ccagtgtttc
     2641 cattattgat caagatctta gacgcagaag atgatctatc tgtacaagta catccagatg
     2701 atgcctatgg gatgaaacat gaaggtgaat taggaaaaac tgaatgctgg tatatcatcg
     2761 atgctgaacc tggtgcagaa atcatctatg gtcatcatgc aaagacaaga gaagaactag
     2821 ctgagatgat caaagatggg cgctgggatg accttttgaa gaaagtacca gttaaaaaag
     2881 gtgatttctt ttatgtacca agcggaacga tccatgctat tggtaaagga atcatgattt
     2941 tagaaacaca gcaaagttct gacacgactt accgcgtgta tgactacgac agaaaagatg
     3001 cgcaaggaca aacaagagag ctgcatatcc aacaatcgat cgatgtaaca actgtccctg
     3061 cgaaaacacc agaattacaa atcaaagaag tccgaaaagg gaattcatcg atcgtcactt
     3121 atttagaaac tgagtttttc aatgtatatg agtgggatat taaaggaatc acatctttca
     3181 aaaaacaagc cccttataca ttgatgacgg taatcgaagg agcaggagac cttgtcgttg
     3241 acggaaaaac atatccatta gaaaaaggaa caagctgtat tattccaaat ggcgtttctg
     3301 agtgggcggt ccaaggtgaa ctagcaatca ttgcgtctga accaggcaaa tagttcccag
     3361 atagctgaaa gcagcagacg gtttttcgtt tgctgctttt tctacataaa atccgtccat
     3421 caataaatag aagtctggtg aaaaagatgg aatacaaatg gaaagcaaac aacagcagtc
     3481 gcgtgtctat gaaacaactg agaaaagctg gacatcactt aaatgaaatg atcatgccaa
     3541 atctaagtct gtttatcgct tggggaatct tgtcattagc aacaaaaggt gtgtctggcg
     3601 atttgcacac cacattagaa gaggtatgcc attggatgat ccagctgctt ttacccatct
     3661 tgatcagtta ttcaggcggg aaatcggtag ggggacaaaa aggggcagtc gccggtgcaa
     3721 ttgcttcttt agggttgatc gctcgttcca atgctccaca aatcgtagga gcgatgatta
     3781 tcgggccgct tgccggcatc gtatatgaac aatttgtttc tagattccat gtgaaattca
     3841 aggctggata tgaaatgctt gtcagcaacc tactagtagg actgactggt agcgttattt
     3901 gtataattgg aatcgttttt attgaaccag ttttaaatag tgttcacttg ttgctcgcct
     3961 ccgttgtgtc ttggctagtc acaatgaatc tgttaccact tgtcagtctg atcttagaac
     4021 ctctaaaagt actatttttc aataatgcaa tcaatcatgg tgtattgaca cctttaggaa
     4081 tcgaattctc acaaacagtc gggtactctt ccttattttt gatggaggca aatcctggac
     4141 ctggtttagg gatcttactt tctatccttt gttttgcaga aaaacaggaa aaagtaaatg
     4201 cgagtggagc attgatgatc catgcgattg gcgggattca cgaaatctat tttccatttg
     4261 tcttgctgcg accgtatcta tttttagctg taatggctgg tggggcaagc ggcacgttga
     4321 tatttcaatg gttagatacg ggattagctg cacctgtatc gcctgggtca ctactcgtga
     4381 tttttgccaa tgcgccttat ccacaactag ctggggtaag tatagggata gcaatcagta
     4441 catttgtgac cttcatttgt gcagcgatac tattgaaatt accaaataaa gaaaaagaga
     4501 acaaaacggt caaagaaagg ggagaaatct tggaaccatc tatcattcat tccgtcattt
     4561 ttgcttgcga ctcaggtatg ggatcaagtg ctatgggagc cgccctgctg aaaaagaaat
     4621 tagcagcaga aaaaatcgat ctttctgtcg gatacagctc tatctatgaa ttgaataacc
     4681 aagtaaatca gctggttatc gtgcaacatg aactagcggg tcttgcaaga gaaaaagtac
     4741 ctcaaagtca aatctatgta ctggatcact ttttggaaac agatatcttg ctgtcacgaa
     4801 taaaagaagc gatccaattt ccaaagcggg aagaaaaaaa agagatagca gatatcacag
     4861 aacaaaaaac gtcgcctgct aatattgtat ttttgtataa aaacaacaga cgcggctcac
     4921 aaactatggg catgacgatt ttaagtcaat tagcaaaaaa agcgaaaaaa cagtttgtta
     4981 tctcaaaaga atcaatagaa aaaatcaaaa tccaagaaga aacgatttat atcgcacaaa
     5041 gagaactagc tgtaactgaa cagctagaag aaaagatccc tcggctagtt ctagttgaac
     5101 attgggtagc tacgaatgaa tacgaaaaac tcgtgaaggg agaaatataa gatgtattta
     5161 tcgcccagag aaaaacaatt attagcagaa tttctcagta gcccgtcacc tgtatctatc
     5221 caaaaaatga tgaatttatt gaaggtcagt aaacggacta tttaccgcga attagataat
     5281 cttgaagtct cattgaaatc ggtaaatgct tctttgaaaa aagcagctag aggctcattt
     5341 gtcattgagg catcagaaga agtattagag caattaaaag aaaatacaga tgggaaaagt
     5401 aatgacttat ccactgctaa acgacaacac gcgattttag ccgagttgtt actgacgaca
     5461 gagccgttga gtcttgctca tttcatggaa tcatatcgca tcagtaatac cactttttat
     5521 gcagatatca aacagttgga agaaagtatt cgccaactgc ctttggaaat cattcggaac
     5581 cagggatacg agatcgaagg accggaaaaa taccgccggc ttctgacagc gaatgtatta
     5641 gaattagaaa tcaatgaata tgaacttttt catagtattt ctttcgatgc atcactgaat
     5701 tatttttttc gattcgttga tccgcaacat ctatctcttg cacgcaaagt aatcggggaa
     5761 gaattgatcc aaaaaaagac caatctaagt gatagaaagc ttgaacattt ggttttgatg
     5821 ctgacattga cgatggagcg tgtgacgaaa aatcatttac tgacaaatga gacatataat
     5881 agtctggcaa acaaagaatt actacagaca gctaaacgat tgttttcacg gatcgcttct
     5941 gagacaaaac aactttatcc agtaaatgag atcgtctttt ttgcaggatt attaaatgac
     6001 ttcacgaact catttgatca gaatttcttt gaagagacgt tcgatacaca gcttgcttat
     6061 ttagtaaaac aactgattca agaagtaagt gaagagacga atgtccattt ttatgaagat
     6121 gaaactttgt acaaaatgct gttgactcat ttatctggtg tgttttcaag agccgtttta
     6181 caagaagaga cactgaccaa tccgatttta gaaaaaatca tggttcaata cgaggaagtt
     6241 gcgaatgcaa tccgtaaagc agtagagcgg atttttcaag aaaaaaaatt atccgaagaa
     6301 gagatcgctt atatggtcct tcattttgcg aattcattgg aacgaagtcc gaaagaaagc
     6361 aatatcagta ttgcaggatt ttcgccaagt ggattagctt ctactagcat gttggaacta
     6421 cgattgaaaa ggtattttcc cttcatccaa caaattcatt tctatcggat tgctgattta
     6481 aaaaaaatcg atttacaaga agaatatgat gttgttattt ctacatctat tttaccagga
     6541 tacggtggaa aatatatttt agtctccccg ctactgctag aagatgaagt caagcagcta
     6601 aaagaagctt tttcgcatat tgagaagaaa aaaaggcaaa aaattacgta taacagcaat
     6661 aacgagtgga tgatagaaga tacttatgag gaaacaatgc atttcatgga tcaaatcaat
     6721 caattactcg gtcgtttttt tatccaagaa ctggataatc ctgagacgat ttccgaaacg
     6781 attcgcagct tatttcctta ttttcctgaa ggactagtaa cggatgaaga ggtggttcat
     6841 cgccggctgc ttaagcgata tgaacaagca cctatcggta ttcctcatac aaatatgggg
     6901 ttattccata catccagctc attgatttct aaaccgatct tttgtatttt caatttgaaa
     6961 catccgttga ccattgaagg gatggataga cagccagtcg atttgtcccg tatgttagtt
     7021 atgctggcgc cttcgccaat ccaagaaaaa gacgggattc ttttaggaaa gatcagtgga
     7081 gcaatcatca tgaatgactt gaacaccgag atcttccgct caggaaataa agaaatcgtt
     7141 tatcagttgt tagcgaaaat cctgattgaa gaaataaaga aatgaaggtg aaaagatgat
     7201 acagctacca gcagaaaaaa tattgctcga tcaatcctat caaaattgga aagaagcgtt
     7261 gaccgagatc ggccagtgga tgacccaaca agggggaatc aaaccggcat atatccaatc
     7321 aatgatcgaa agacaagaaa aagcgagtgt ctatatcggg aatttcgtag ccttgccaca
     7381 tgggactgac aaagatgaag aagtagtaga agaaggaatc tatttagtcc aagtgcctga
     7441 tggtgtggac tttggaacaa aagaggaacc aaaaatcgcg acactcttat ttgtagtggc
     7501 tttaaaaaaa gaacatcagt tagcggtgct ccaagattta gcctttttct gttcagacat
     7561 cgaccaagta atggcgctca gtgatgcgga aaccattgaa gaagtccaaa tgatttttga
     7621 acatggacat atataaccaa tgttcacagt aaaaaaagct gccttgcatc aagtaataac
     7681 ttcaaccaag ataaaaataa gaggagctat ttttgttggg aggttcttat tactgcggat
     7741 gcaagctttt gaaaactatt tattctagaa aaccgtttat ttgaaatagg ggacggtgac
     7801 aaggtttttg tcaccgcccc tattattttt gtgttttaat agctcttttt tatatagata
     7861 gtgcccatac ctatgaataa taaaacgata ctccatatac caagtgtcaa gataccattc
     7921 atgaaagtaa cctgttctga agctagtggc gagaaaatat ctcctcctat gatcacattt
     7981 tgaaaatcaa cataagaagt tggcaagtaa tgactgattg attctatcca tccaatcgaa
     8041 gaaaacaagc gatactctgc taagaaaaga caaagaagtg tacctaaaag attgactaga
     8101 gaatgttcaa ataaagcaga tagaagaaaa ctgattgtac ttaaaaagag cacccacaac
     8161 aacaggaaca gaaagctctg gaaaagatac tcatacatcg gcaggattcg tatctcctga
     8221 ccaatgatcg ttcctacagg ataacggaag tctcccaaac catttttgat tattactagt
     8281 atagagacga tcaaaaatgg catcacgaga cttagcatag ctgcaccata ataaatggta
     8341 aaacggatgc tatgtttctt taccatacta tccggtaata gattgactag agagattacc
     8401 tgctttcttt tttcataaga gatgatggaa gaaagaaaca aggcggttat acataaaatc
     8461 aaaaaactag aaatcattcc tgaaaaaata ttttcgtagt aatttgctaa agagagatgc
     8521 gctggcaagt caaaaataga atatctttgg atctgatgtt ctttcatata acggagcaac
     8581 tcgacacgtt tttgttgttc aatgattgga attccttgtc tttgaccaga aatcagtcga
     8641 tccagtatat ctttttcata atgataagtt gcttcgacca tttgctggac attccctgtg
     8701 ttttttgcat gtaagattgc ttcgatcagt ttgttgcttt tctctaaact ttccacaaat
     8761 tctttctcat tctcatcttc tttcaacgtt tcaatcaaac gttgcatctc ttcatagtta
     8821 tgattcagtt catcgaattc tgtataagca agtttatgat caaaaaaaag tgggaatgca
     8881 aagagcacga ccaacaatag aggaaataaa aagtttactt tgttttttag taagagattg
     8941 cgttcaaatg taagtaaaga catagttaca cctcatataa tttacgataa atagctaaaa
     9001 tctcttccat atttgaggat tctgtgacga tttctccatt ttttagaaaa catgctttag
     9061 aacaaatcga ttggatattc gcgatagcgt gggaagaaag aagaatcgtc ttcccctctt
     9121 tctgccattg tctgatgagg ctattcaaga tatcaatact tcctgggtct aatccgttca
     9181 acggttcatc gaataacatg acagatgaat cactgatcaa gtaagcagca agcaagacat
     9241 gttgtttcat tcctagagac atttttccaa tcggcaaagt aaggtactca gtcatcttca
     9301 atcgttggaa aacagtttgg cattctactt tgctctgcca atagtatttg atcaaagtca
     9361 aataatcaac agccgataga tagtcggata actgattcgt tgattctaga aaaaagaaag
     9421 accggcgata atctgtataa gcagtaggag agatgttgtg aagggacaat tgatcataag
     9481 ttgttggcag cagttgggca atgccattca gcaaagttgt tttgccaaat ccgttcgggg
     9541 caatcaaagc agctacttct cctgattcta ctaaaagatt caattgtcga atcacacgtt
     9601 ttttaccacg tttcatgttc atgtttttga tcagtaatgt catagatagg cctccaatat
     9661 aaaatttaat aacataatag aatggaaaac atctaaatta aatgctaaca aaagttattg
     9721 tttgcatatt ataaaaaaat taaaataaca gttttattta aaattgaagt tttctgatac
     9781 cgaaatcttt ttattttgct gacatactca aacctttggc acaaagtttc gtgccaaagt
     9841 acgactattt ctctttttag taagcggata caattagagt atcaaaagga gagagggaga
     9901 gacaaaatgg aacggacgat tcccaaaaac aacatttcgc taaaagcgag ggtacaaaaa
     9961 ttaggcagta ctttatcaag catggtcatg ccaaatattg gagcactgat tgcctggggt
    10021 gtattaacgg ctctatttat cccagatggt tatttaccaa atgaggcttt cgctaccatg
    10081 gtaagcccaa tgctaactta tctgattcct ttattgatcg gttatactgg cggaaaagta
    10141 attgctggtg accgcggatc agttgttggg gcaattgcaa cgatgggtgt gattgtcgga
    10201 acagatattc caatgatgct aggcgcgatg atcatgggac ctcttggtgg atatgccatc
    10261 aagaaatttg atcagctatt ccaaaaacgg atcaaatccg gttttgaaat gctagtcaat
    10321 aatttttcag caggtcttat cggttttgga ttagcgttat taggtttttc tgcgattggt
    10381 ccagttgtcg atgcattgac tcaagcaatg gcaaaaggtg tagagatcat tttatctgcc
    10441 catttgattc cattgacaag tatttttatt gaaccagcaa aaatcttatt tttgaataat
    10501 gcaatcaatc atgggatttt gacaccattg ggaactgaac aagtagcaga ggctggaaaa
    10561 tctattctat tcttgcttga agcaaatcct ggtccaggac ttggcgtatt acttgctttt
    10621 actttgtttg gcaaaggagc agcaaaatct tcagcaccag gagccatgat cattcacttt
    10681 ttaggcggga tccatgaaat ctattttcct tacgttatga tgaaaccgtt attattctta
    10741 tcagttatag gcggaggaat gtcaggaagt ttcattttcc aattgatgga tgctggacta
    10801 agagcgccag cttctccagg gtcgatcatt gcgattctag cgatgacacc gttcaattct
    10861 taccttcctg ttattttggg cgtgattgtt gccgcagctg tttcgtttgg tatttctgca
    10921 gtaattttga aagcggatgc gaaagataca aatgaagact tcgaaaaatc tgttcaagag
    10981 acaaaggcag ctaagcaagc ttccaaaggt gcccaaggta ttcctcaagg agctacattc
    11041 tctggcgtac aaaaaatcat ttttgcttgt gatgcaggaa tgggatcaag tgcgatgggc
    11101 gcttcgatct tgagaaaaaa agtacaggaa gcagacttgc ctcaaacggt gaccaatcaa
    11161 gcaatcagcc aactgacaga tgactcgaat acattgatcg ttactcaggt cgaactacaa
    11221 gaaagagcaa aacaaaaagc accgagcgcg cgttttgtct cagtagaaaa ctttttgaat
    11281 tcacctcgat atgatgaaat cattcaagaa ttaagcagca agcagaaaac atcagtttct
    11341 gaaattgaga atgagccagc agaagagttt ctttcagcaa acatcaaaca aatcaaagag
    11401 attctacttg tccacgatga tcgttccgga tcagcaacaa tggggatgac cgtgctcaaa
    11461 gatatattag acaagaacca tttaactatc ccgttaagaa aagtcaatat ccaagattta
    11521 aaagatgaac ctgattcact gatcgtagcc aaagaaagtt tagcggaaaa agtacagatg
    11581 gctgcacctc aatccattca tttatcagtc aacagcatgg tcaatccgca aaaatacgaa
    11641 tcgattgcac aatatttgaa acagacagct taagggagga aaacagcatg caaagtttgc
    11701 cattcgacat gatcgagctc aatcgatctt ttaaaaccaa agaagaagcg atccgctatt
    11761 gcggtcagat gcttgtagaa gcagggtgcg ttgatgagga atatatcgat gccatggtag
    11821 atagagacaa aatgttatct gtttatatgg ggaattttat cgcgattcct catggaacag
    11881 atgaagcaaa aaaatatgtg aaaaaatccg gcatctgtgt cgttcaagta ccagatggtg
    11941 tagattttgg taccgaagaa gaagaaaaga ttgcgactgt tttgttcggt attgcaggag
    12001 tcggagatga gcatttgcaa cttgtacagc aaatcgcact ttattgcagt gatatggaca
    12061 atgttgtcca gctagcagac gcgctcagca aagaagaagt aacggaaaat ctagcaattg
    12121 cttaaggaga gagaaaaatg agagcagtac attttggtgc aggaaatatc ggtcgaggat
    12181 ttattggaga ggttttggca gctaatggat ttgagattac tttcgtagat gtgaatcaca
    12241 cgatcattga tgctttaaat gaaagaaaag aatacgaaat cgaattagca gatgaatcaa
    12301 aagaaaaaat cactgtaaaa aatgtcaaag gaatcaacaa tcaaacaaat ccagaaaaag
    12361 taatccaaga aatcgctgaa gcagatttag ttactaccgc cattggcccg aatatcttac
    12421 cttttattgc tgagttgatt gcaaaaggta tccaagaaag agaaaaggaa ggaagcacaa
    12481 catctttaga tatcattgcc tgtgaaaata tgattggcgg ttcagcattt ctagaaaaag
    12541 aagtgtataa atatcttccg gaaacatcat ttactgacaa atatatcggc tttccaaatg
    12601 cagcagtcga tcgaatcgtc ccattacaac accatgaaga tcctttattc gtccaagtgg
    12661 aacctttcaa agaatgggtc attgacgata gccaaagaaa aaataaaaaa atccaattaa
    12721 aaggggtttt atatgtagat gatctagaac catatatcga acgcaaacta tttagtgtca
    12781 atactggtca tgcgactgtc gcttatacag gtgccttgtt aggttaccag accattgatg
    12841 aagcaatgca agatgcgtta gttgtggcac aattaaaatc agttttgcag gaaacaggaa
    12901 gtttgctgat tgccaaatgg gggtttgatg cagagcagca tcatgcgtat attgaaaaaa
    12961 tcattcatcg cttccaaaac aaaaatatct ctgatgcgat cacacgtgtc gctcggacac
    13021 cattacgtaa gttaggttat caagaaagat ttacacgtcc agtgagagaa ctacaagaac
    13081 ataacttgac ttgcccacat ttgacggcaa caatggggat tatttttaat tactatgatc
    13141 ctgaagatga acaaagccgt caattgcatg aaatgaagat ccatgaaaac ttggaacaat
    13201 tgattcaaga agtcacagga atcaatgatc cgaaaacaat cgggaatatc aagcaaaacg
    13261 tgaaccgtta tgccaagcaa gtcgcttaaa ataggatacg aaaaagggga atgtgacgaa
    13321 atttttgtca cattcccctt tttcgaataa ctaggctgtt tgtccttcaa gatagggcag
    13381 atccaattct ttaccgtaac gttgttctaa agctaggcgg atcaaacgag ctgccaggtt
    13441 ctcatttctt gcaaggatag gtccatggaa ataagaaccg aagacatttc ggtaaatgac
    13501 accttcagat tggtcttcac cattatttcc ttgaccttgt accactttcc ccaaaggtcg
    13561 ttcaccttca cctaagaaag tccgaccatt gtggttttca aatccataat aagtttcatt
    13621 gaattcttcg ttatggataa cgatatcacc gataaaccgg ttattttcct gacttaacgt
    13681 gtagtgatcc agtgcactga ttccttggat cttttctcca ttagctccca tgtagtagtg
    13741 acctagcaat tggtagccac cacaaatggc taacatgacg ccatcatttt cgatatagtt
    13801 gatcaaagaa tctttctttt tctggatgtc ttttgaaatg attaattgct caaagtcttg
    13861 accaccgcca aagaaaacca agtcatattt atcagcatca aaaggttcat gtatactaac
    13921 gatctccgta tggcaagaaa cccccatttt ttctgctata tatttcaaca tcaaaaggtt
    13981 tccgttatca ccatatgtgt tcatcaaatt tccataaagg tgggcgatat tgagttcata
    14041 attagccacg gttcattcct cctttgataa atccttgagc agctagttct ttacgaagct
    14101 gcaatacagc agtgtaagta gctaaaatat agacatgctc agttggcaat tctttgattt
    14161 tttcgattac ttctggtaat tcttttgttt ctgtcaattt attttctgga atcccggcaa
    14221 cttttagacg taaagtcata tctgtatgac ggtctcctcc agaaataaca gctgggatat
    14281 ccatatcggc aaagttttca aaattaccat cccaaatcca gctgacatcg attccgtctg
    14341 cgtaattggc atttaataaa gccactaaag aaaatggata aggagctaga ccaatcatat
    14401 cgattacttg attgatacct acaggatttt tgacaagtac taatgtacat tttttcccat
    14461 tgatattgat gacttcttgt cggccgaata ctttctcgtc atatcctaaa ccttgtttga
    14521 ttttatcagg atcgacattg aagtactctg ccactgcagt agctgctaaa gcattgtaga
    14581 cattatacat accgccaact tcgattccgt attcatgacc atcgatcacg aaatctgctg
    14641 acacattgtc catccgtacc atatcagtta atcgataatc caattcagga cgtttgaagt
    14701 cacaatttgg acagtaatat ttccctaagt ttgcataagt gatcatttta taatgcaaga
    14761 tgtgatgaca atttggacat aagacaccgt ctgtattgta atgagccatt tgttcacgat
    14821 cttcttcatg gtcaaagcca taatattttc taggattgac tgtttcgata gaattgaaga
    14881 ttggtgaatc accgttactg ataatcgttg catttggtga ctttgcggca ccgtctacaa
    14941 tcattttata ggtcgtatag atttctccat accgatccat ttgatcacgg aaaatatttg
    15001 taaataagaa cagttctggt ttcatatatt ctgtgacttt gctcaaactt gcttcatcta
    15061 tttctaggac agcaaacttt ttctgtcctt tttttggttt tgcctgcaag aaagtcgata
    15121 caattccctg taccatattc gcaccagtag ggttggttag aacttcatcg aattgctggc
    15181 gcaaaatatt gactgttaaa gcggtagtca atgttttacc gtttgttccg gtgacaacga
    15241 cgacttggta gtcttttgcc aaactatcta gtatatgtgg atcgactttc agcgctagtt
    15301 tacctggtaa acttgatccg cctttaaaaa aagtttttaa tgcccattgc gacgtttttc
    15361 caactgcaat cgcaaaatga ctgcgtattc ccattatgta acctccaaaa acgatttgct
    15421 gacgaataaa ttcaactaat cataccatat gttgaaaaaa aggttaaatt gcttttctta
    15481 ttcttaagaa aaggttacgg ctgaaagtgc atgaaatcgg acaaaatcgc atgatttaac
    15541 aaaaaaacag cttgaaacaa aatgattgtt tcaagctgct gtgattttat tattccctct
    15601 tacctatatt cccattttaa ccgataataa ttttctcgct tggatattca tagcctagat
    15661 ctcgtgtttc ttttggcacg aataacatca atgtatatag cattccgatc cgcccaataa
    15721 acatcagtgt agcaatcgtg attttgccca caacagacaa gtctccggta atccctaaag
    15781 ataaaccagt tgttccaaac gcagaagtta cttcgatgat gatcgagatc agcgtttgtt
    15841 cttctgtagc agataaaaaa acgatacaaa agaaacacat accaagagaa agcataaaga
    15901 caacgactga ttttcgcaca tcatcttgat cgattctccg tccaaaaata ttgatgttat
    15961 cttcactttt gaggaaggaa tatagataca aaccgatgat tgctacagta gttgttcgaa
    16021 tcccgccgcc tacagaacta ggactgcatc cgataaacat cagtaaagaa aaaatgatca
    16081 gtgttgtgat ttgaaaatca cccaaatcgt gaatctgcag accagcatta cgagtcgtaa
    16141 tcgaataaaa catcgaattg atccatttga cactcatatt ggaatcttgg aataaatggt
    16201 ccttttccaa tagataaatc aagacagttc ccgaaacaaa caagataaca aaagctaaca
    16261 cagctaattt ggtaaacaag gaaaaacgaa acggaagctt cgcattggaa cgtttgtata
    16321 atagccattc ccggcactcc atcaatacag gaaaaccaat tcctccgaca aaaatcaaaa
    16381 acatgatcag gatcaagaaa aaatagtcat gtgcaaaggg cttgatggaa tccccagtga
    16441 cgtcaaaccc agagttcgtg acagccgata tcgcctgata aaaaccgtaa aaaacggcat
    16501 cacgccaacg atcaaaatag cctctgtaat aaaaataaat agaaaagaat gtcccaaaaa
    16561 gtaattgaaa ccagattaaa atagcaaaag tgatccgaat cagtcggacg ataccagaca
    16621 aacgcggttg attcatatcc gtcatgatca gctgtcgctg tttcagtgtg atccgtcgtt
    16681 tagaaaaaat gacaaaagca gtagaaatca tcatgatccc caaaccacca acttgaaata
    16741 atatttccag cagtataata ccgttatcat tgaacacaga attgatatca aaagtcgata
    16801 gtccagtcac actcactgta ctgattgcca tgaacagcat gtccacaaaa gaaacatggc
    16861 tatctggttc gcgaaaaatc ggcatataaa atagaacgag tgataagacc gtcatcaata
    16921 tataataaaa aacgatgatt tggatcgatg agaaatgatt ggcagcaaac ttctgtcctc
    16981 gccgctgcag ccttcgaaag aataaatcaa catgcacagt actatcctcc tgacaatagt
    17041 cgtttctacc agtatacaag gggaattagg aatactcaaa gaaattatca aacagataac
    17101 aatacagctt tcgattaaaa tagaaatggc ttataataag gaaaatccta gaaaaaagaa
    17161 ggaggaggaa taacaaattg attaatgctc aacaaatcgt agaaaaaatg aaaaatcaaa
    17221 aaataaatta tgatacagtt ctgaaaaaaa tgatcatgca atgggagaaa gaagaagtaa
    17281 agcctaagat attaatacat acatgctgtg ctccttgcag tacgcacaca ttggaattca
    17341 tgtctcaata tgcagagatc acgttatttt tttcaaattc gaatattcat cctaaaagtg
    17401 agtatttgcg aagattacat gaacaaaaac gatttgtaga ccaatttaat gagaaaactg
    17461 accatcatgt tgcgctaatc gttgatgaat accgtccaaa tgaattcacc aaaatggttt
    17521 taacaaacca tcttgaaaac gaaaaagaag gtggtgctcg ctgcagtgca tgcttcaata
    17581 tgcgattaga tcttgttgcg caaaaagcgc aagaactagg atacgattac tttggaagtg
    17641 ccctgaccat ctcgccaaag aaaaatgcag ctttgatcaa tcagatcgga atggatatcc
    17701 aaaagattta cgggacacaa tacttgccaa gtgatttcaa gaaaaacaaa ggctatgaac
    17761 gctctttgca aatgtgcaaa gagtatgata tttatcgcca atgttattgt ggttgcgtgt
    17821 tcgctgccaa gcagcaagga tgcgatctga aagaaatcaa tcgggaagca aaagcttact
    17881 taaaaacatc aaccgtagtt tttgaggaaa attaaagaaa tctttcggaa aaacgtaaga
    17941 aaaaaataaa gaatttatgg tagaaataag gtagactgta taatttgtcc gtagtaaagt
    18001 aaggaaacaa gaataaattt caatgaagga agagataaga tgcgaaaagt agcacctatt
    18061 ttgaagccaa tcgctgtatt aacaatctcg acgattgtcg cgactactac aggtattgtg
    18121 aatagttcca gctaccgtgc accaagttca agttctagta ctgtagaaga aacaacacca
    18181 actacttcag tctcgacatc tgaaccaaca acaacggaaa gtttgaatga aaccactacg
    18241 acaagtacat gggacgatac aacaactagt tcgtcgtatg atgagacata tacaactgac
    18301 agctcaagcg aaacaagtaa tacggtacca gatggcactc catcggattc aaccaatcca
    18361 agtacaagtc cggatacaag tgctagctcc gaaacgatac caagcagtac gactgtagat
    18421 gataacacgc aaaatgatga tgcagcacgt acagcacagc aaaaccaaga ttcaggaaat
    18481 acccaaacgt ataaccagca agcaggtgat tctcaatgac atttttacta gaccttatca
    18541 acggggtgca taaattcctt ggctatttag acatcagtcc taaatatctt aaccgtggat
    18601 atacgatttt aagtgtgatt cccacgcttt atttattgag aattgtttat ggactttggc
    18661 aaaaccaaaa ctatcttcaa tttttcttat atggacttgc atttctagtg cttgtttatt
    18721 ttactgtttt aaatgtattt tattactttt tagataaaaa cacgaaagcc gatgtcactc
    18781 agctttttgt gaagtattta cctgatgaag cgttcaacat ccagacagaa gaatcgatag
    18841 ataatggaaa ttcgatcgat aaagtgaata cacaagaggt catggtttct tatgacgaag
    18901 attatcaatt gaaattagcc gagaatatgc ggtatttgat tggaaatggt gagatcaaaa
    18961 caaatgaact cggtcgcatg gatggatttc tggtcgatag aaatacattg tatccttatt
    19021 actatgtcaa gaaaacagga gagaaagagt atgttctaaa tattggacga agctactcgg
    19081 acatggaaaa agtagggacc attcgacttg actcaacaga agacacattg gaacctgtcg
    19141 gattgttcat cgtcggcggt gattttgtca aagacgggtt acgctatcat gagccttatc
    19201 gtctgaaatt gattgcaaaa aaggcacagt cgcaggaaga accagtggtt tatagtcgta
    19261 gccaaagaag aagacattca taatgtgatg aaacgccgtg tatcagtggt tgcgataaaa
    19321 acgtttattc ggaataaggg ggatgtgaca cgtcttttgt cacgttcctt ttttatataa
    19381 cgagaaagat aagacgaaca catcacttat tctttccttt catttataaa cattgtagaa
    19441 taaagaaagt acaacgaagt attgtgaaaa aacgtaataa ataacgagga aaatacaagt
    19501 aggaggtaag ctgtgaaaga atttttgaaa aattggggga ttttgatcct tgtattagcg
    19561 gctattttat tagctagagt gtacgtgttt acaccagtta ctgtaaacgg gcattcgatg
    19621 gaccctacat taagtgatgg acaacgattg atttcatcta agatctcaaa ctatgaacga
    19681 atggatatta ttactacaaa ggaaccgggg gatgaagaac ggatgattgt taaacggatc
    19741 atcggtatgc ctggagatac agtcaagatg gaaaatgacc agttgaccat taatggcaaa
    19801 aagtatgatg aaccttatct ggatgagttc aaaaaagaat tttcagaaga taagttacaa
    19861 ggagaatatg cttatagctc aggattccaa gcacaagcag aaagttcatc cacctttacg
    19921 agtgactttg aatatactgt gccaaaagga aagtatcttg ttctaggcga taaccgcttg
    19981 atttcaaaag acagccgaat gtttgggtta gtcgacaaag atatgatcca aggaaaagtt
    20041 gttttccgtt attggcctct ttcagaaatt aaaatcatgt gatgaaaaaa taaaaagaag
    20101 ctgtgacatg ttttttgtca cagctt
//
LOCUS       DACGLW010000052        19740 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.054, whole genome shotgun sequence.
ACCESSION   DACGLW010000052 DACGLW010000000
VERSION     DACGLW010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.054"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            complement(125..1705)
                     /locus_tag="IXT53_001868"
     CDS             complement(125..1705)
                     /locus_tag="IXT53_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HAQ4905673.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(1698..2441)
                     /locus_tag="IXT53_001869"
     CDS             complement(1698..2441)
                     /locus_tag="IXT53_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4905674.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(2617..3393)
                     /locus_tag="IXT53_001870"
     CDS             complement(2617..3393)
                     /locus_tag="IXT53_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ4905675.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(3641..4477)
                     /locus_tag="IXT53_001871"
     CDS             complement(3641..4477)
                     /locus_tag="IXT53_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HAQ4905676.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(4488..5417)
                     /locus_tag="IXT53_001872"
     CDS             complement(4488..5417)
                     /locus_tag="IXT53_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HAQ4905677.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            5609..5962
                     /locus_tag="IXT53_001873"
     CDS             5609..5962
                     /locus_tag="IXT53_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAQ4905678.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(6036..6692)
                     /locus_tag="IXT53_001874"
     CDS             complement(6036..6692)
                     /locus_tag="IXT53_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAQ4905679.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            6918..8852
                     /locus_tag="IXT53_001875"
     CDS             6918..8852
                     /locus_tag="IXT53_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ4905680.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(8938..10161)
                     /locus_tag="IXT53_001876"
     CDS             complement(8938..10161)
                     /locus_tag="IXT53_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronyl hydrolase"
                     /protein_id="HAQ4905681.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(10155..12053)
                     /locus_tag="IXT53_001877"
     CDS             complement(10155..12053)
                     /locus_tag="IXT53_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HAQ4905682.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(12047..13165)
                     /locus_tag="IXT53_001878"
     CDS             complement(12047..13165)
                     /locus_tag="IXT53_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 88"
                     /protein_id="HAQ4905683.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(13248..14081)
                     /locus_tag="IXT53_001879"
     CDS             complement(13248..14081)
                     /locus_tag="IXT53_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ4905684.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(14074..14991)
                     /locus_tag="IXT53_001880"
     CDS             complement(14074..14991)
                     /locus_tag="IXT53_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ4905685.1"
                     /translation="MIQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(15218..16342)
                     /locus_tag="IXT53_001881"
     CDS             complement(15218..16342)
                     /locus_tag="IXT53_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ4905686.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            16525..17433
                     /locus_tag="IXT53_001882"
     CDS             16525..17433
                     /locus_tag="IXT53_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ4905687.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            17574..19187
                     /locus_tag="IXT53_001883"
     CDS             17574..19187
                     /locus_tag="IXT53_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HAQ4905688.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT         6297 a         3836 c         3236 g         6371 t
ORIGIN      
        1 ttttgtaaaa atagcttctc acatttttac ataaaattga cttaatactg agaaactgac
       61 ttttgaacac cctctatctg taaaaaaggg gctgcgacat gatttttgtc acggcccctc
      121 ttctttattc aaattttttc caaaacgttc gttgataata aacgatccat agagcggaaa
      181 gtattagaac taaaccgatc acaggaatat tcactaacca aaatggataa aacaaagcca
      241 acattccgta taagacgata agaataatac tgatgatcaa tgaacccatc atagttgcga
      301 ctaaacccaa acttcctgaa cctctgttaa ataattggtt aatattggtc cagttcagca
      361 gcagcaaccg ataatctcta gcgaaataac gtagtgacaa taagtagcag ccgaataaag
      421 aaccaaatag caagctaggg ataaaaaaca aaggcagctg aaaagacagt ccgcttaata
      481 atgcgatccc accagttaaa aaactctgca atatccatcc gaatctgaac ttttctttta
      541 aatattggtt catagagatt ggcaaagaac ggataaacag aaaattttct tgatccaaag
      601 aaataagatt gctgataaaa gaagtctgat taactgttaa gaaagccaat gcgatcccta
      661 ccaggaaaac aacaccgatt aacttattat ctaaatcagt taattttatt tcgcctccta
      721 gggcaaaagc aagaataaaa atgattggag ttaacaagga agaagaaaga acttgcataa
      781 tcaaattagg ttcttttact aattgagcat tgtaactaaa taaaagctga tgcacatttt
      841 ggttcttttt atgggttctt tttattttga actcactgtt tgaatcagtc agttgctctg
      901 taagctttgg tactatcagc tgttttacag caaggaaaaa tcccagagcc gtgatagcta
      961 gtattcccca acccaataaa ccttgttgag aaagtggctg actggcagca tcatagaatg
     1021 gcaataatat cgaaatcgga tgccgatctg ttaactcatt ttcaatagtc cccgtttgat
     1081 gattcatcca catgattcca gcaaatgcga tcagcatcga gctgccaagc aagatactag
     1141 aaactgtttt tctatgtttt ttaaacactg ctgttcgagc caaaccaaaa acaatcaaac
     1201 tagccagaca aaaaagaata atcaaaaata gagaaaacaa aaggattgag ataatgatag
     1261 taagcaaaat ccctagatca gctcgccatc cggttaaaat aaacatgact agaagcggaa
     1321 aaacaaaagg aatgatggtc aaagcaacta ccaaaatctt tgctgaaaaa acctgttctt
     1381 gtccaaaagg cagaggcaaa aatacttgca ggtcatttcc ttcaaaaaag atattataaa
     1441 tgactgatat actttgagtt aaacctaaca taccaaaaag tgccacgtaa taagtaaaga
     1501 aacctggcaa tttaacaaag ttcatagcaa acatagcaaa tccgtaaatc agtaaaaaaa
     1561 tcagccctga aaaaagattt tgagacaaaa gatagatagt taatcgcttt cccttttttc
     1621 ctttctctct tgctttattc gtgagctgtg gattaacata taaaagatta actcgaatta
     1681 attctaataa ttgtcgctta tacatacggt ccatctcctg tcgcttgcct tccagccatt
     1741 ttcaaataaa tcttttctag cgtttcatca ggtgagccat tcaataattc actaactgcc
     1801 ccctgataaa tcaattcacc tttcttcaaa atagccagtt tatcgcataa ctgttgtgcg
     1861 gtatccaata catgtgtaga aaaaatgact gtttttcctt ttgcggcatg agccttcatc
     1921 atttctttca aatcaaatgc tgcttgagga tcaagtcctt gcaaaggttc atccaatacc
     1981 caaatatctg gatctggcag caacgtgccg atcaaaatcg ttttttgacg cattccatgt
     2041 gaaaaacttg cgattgtttc attttgatga ctatacatat caaaaagcgc tgtcaattct
     2101 gctaaacgct tttctttttt ttgtgtgtct aattcatagg cagctgaaat caagtcccaa
     2161 tattctccag ccgtcaattg taaaaaaata tctggggaat caggcacata accgattttt
     2221 tgtttgatac tcaatcgatt ttccgtcaat ttcattccat ctactaaaat cgttcccgat
     2281 gatggttcaa taatactaac taaactttta atcgtagttg attttccggc accattatgt
     2341 cctaaaaatc caaaaatttc cccttgtctt atcgagagag acaagtcttt taatgcttgt
     2401 tttttcccgt aacttttact tacattgatg aattcaatca tttctgtact ccttttgttt
     2461 gttattactt tttattgtac ccttcgttat tggtggagta aatgtcaaaa agagtgcata
     2521 acaaaaaagg atagaaacga ctaggctgct acttgggttg ttctcttcta caggaaaaca
     2581 ctcttctaac aggtaagata tgagtctttg ttttctttaa aatgattcat tttctatctt
     2641 ttcttgcata aaattcttta gtttatcaat acttctcttt cccatctcta gttgttctcc
     2701 atttgcaaat gttactattt cgtttactct gctaatagat gtaatgtttt cagtattaat
     2761 gatataagct tttaaatctt tacataaata ctcatattcg agtgattttt tatatttttt
     2821 aattgtgccg tttacgagaa tctcttcatc tttcaatact aggcctattt tatttttata
     2881 acctggcatg ctcattagat aaaggatttc atcgtaatgg atatagtgat cgctattacc
     2941 ataacggaca gttgtccacc tttttttcgt agcatgttga acaggatagt tttctttttc
     3001 tttcaataag taactttgga tgcttttttt atttttttca taagtgaatt cttttatcaa
     3061 ataaccacta ggcgtaatct ttcgattaat aacagttatt gctttattcg gatgagacgt
     3121 tagataaaca attttacact tttcattctt ttctctaagc atttcgccta gttcgatacc
     3181 gctataatag gcgttcaaat cgatgtctag gaaaaaaata tctgaggaat agatttcatt
     3241 atttttaatg aattcaacaa attgtataac gttcaatccc tgaacgatct caaatttcca
     3301 aggacttaat aaaattctgg attcttccaa aatttctact agcatttttt caaaaaccaa
     3361 actatcatcc aaaataaata tacgcgttac cataaaaagt acctccactc gatattttac
     3421 tatcaaaatc atttcataca acttttcaac atacatgtta ggaataaatt gtaattctct
     3481 tcacaatctt ttccaacacc tttgaaaccg atatcaaata ctttcaggat gctgcttcta
     3541 aaataaatat caaacaaatg aatacatcat gcaaaaaaag ccagacaacc gacgtccttg
     3601 cgtgtcagct gtctgagcta tgtttattta ttttgttctt ttaaaaagaa cgatacacat
     3661 actgattttt cggagcagag actttctcag cgtttgtaac aactacagat gggatttcac
     3721 gcttgaatgg ttgataatag tctgattgga tggttccttc tattttatac cattcatcat
     3781 ttttaaccgt tactccttcc ggcatatgcg tcaacaaacc aaagactcct gaatctgcca
     3841 cacaatggat aatgccgaat cggaacaaga agatatctgt attgctatcg ttcggtgttt
     3901 ggtaaacaaa tccttcatag ctgatttttt tgccaatgaa ttcgctaggg taattataaa
     3961 ttagctccat aacttctaaa tagttttcat ctgtgatctt gatagtttgc ttatccccat
     4021 acttttcttt caactcattc atttggtcta agtagtctga tttgttgaaa taaatactgg
     4081 tatccggctt taagtattgt gtctgcatct ccggatcccc tactgattct ttacttactg
     4141 ggaaattaaa tcctttggct tctacgatcg tagtgtcgag actaactgtt ggaaacaacg
     4201 tccccacgat tactggcaaa gctagcagta cataagcgat tccacgttga taaggttttg
     4261 acaacccatg gtcatgatcg tgctcgtgtg ccttttcttc tgcttttccg tttttgaccc
     4321 acaaatacag ctgaactaaa gccaaaagag cagataaaat cattgaaaga atagctaagt
     4381 agcgataatg gacattgata tattgatcga gtttgccaga aacttgcaag tacatcatca
     4441 attcgaaata cccagataaa atcaaaaacc gcagcatatt ttccctccta aataaataat
     4501 gtgaatccaa taacgctaag ggcaatcagt ccgatcagcg ataaaataaa gcgtcctgta
     4561 aaatatcgtt tcatcatcag tagatttttg atatcgacca ttgggccaaa gaccaaaaag
     4621 gcagctacag gtgctgtgcc gaacagactt aacaaagaag atcctataaa cgcatctgct
     4681 tctgagcaaa gagacatcgt aaaggccaat aacagcatga tgataatggc taaaatcttt
     4741 gttgagccga gcgttagcat aattctagtt ggtaaataag tctgcatcgc agctgcgatt
     4801 aatccgccaa tgatcaaata acgacctgta tcaaaaaatt cgtcgattga atgtgtcaac
     4861 acatgcccag tttgatggat cagctgattt tttggttcat tcttctcact ttgttcttct
     4921 tcatggacat gatgatggtg attttctgct actttagacg tgattttttc tttcaataca
     4981 gcgtcttttt gaaaataggc aagccagaca ccaatcacaa gggcaacaaa aaagctgcct
     5041 atcacccgcc agcttgccat ctgccaagaa ttgccaaatg cgataaaagt ggaaaaaatc
     5101 acgattggat tgatgatcgg agcagtcagc ataaaagcaa acgctgtgtg aactggtacg
     5161 ccttttttaa caaattgatg aacgatcggt acgatgccgc attcacatga aggaaagaaa
     5221 aatcctaaga tcgaaccagt aaaaatagag aagatacgat tttcaggaag ccatctcttc
     5281 acacgttctg gggtcaaaaa tacttgtaag gcgcctgaga taagacagcc taataatacg
     5341 aaaggtaatg cttcaataac gatcgataag aaaatcgtac tcatctgcaa aaccgaattt
     5401 ggaataaaag aaaacatgag tttcctcctt tttatttcta gttcattcgc tttgcaaata
     5461 tacgctttta ttcaaaaaaa acaagttgct ctgtaaattt ttacacaact cttatttttt
     5521 accaaagcac ttcaattgac tttttctgtt attcccatta aattaaggtc tcatacatat
     5581 cacttctatc ataaaaggga gaataaaaat gaaacaaacc actaacttga ctgaagttca
     5641 agacatactg caacagtcat ccgttgccgt catttatctt tcgatgccta attgcagtgt
     5701 ttgtcacgct gttcgtccac gactagagga acttctcaca cattatgata ttccggcact
     5761 tcatttggat gcatttgaaa caccagaagt agctagtaaa tttgaagtgc tgactgcgcc
     5821 agttgtcctg atttttcatc aaggaaaaga agtctttcgg caagcacggt tcatcgactt
     5881 taaaaaaatc cgttatctac tagatgaact gacagcagaa gatgattccc tttcttatga
     5941 agaaatcttc cgaacaaaat aatcgttgtc caaacagaaa aaagcgattg aacaagtgaa
     6001 agttttgcct tcatttgttc aatcgctttt tatttttatt ctgctgtatt ttcggaatgc
     6061 gcatttgcgt cagaatgcaa gaaatagatc aaggtttgca attcattcgt caaatcaaca
     6121 ttttggatca atacatctga cggagcattc aatcgagcag cggtaaagtt caagattcct
     6181 ttgattcctg cttccgttaa ctgattgact acttcttgtg ctctttcagc gggtatcgtt
     6241 aaaattgcta cttcgatttg ttggatcttg atttgttctg acatatcttc cattggataa
     6301 acggggatac cgtctacgat gcgcccaacg atttcctcat tcacatcaaa agcacaactg
     6361 atacgtatgc tattactttg atggaatttg tattttagta aagcacttcc caagttccct
     6421 acaccgatca gcgcaacatt tgtcaattga tcgtcgttta atgtctttgc aaaaaagtgc
     6481 ataagatctt ctacatcata accataccca cgttttccta attcgccaaa ataagaaaaa
     6541 tctcgacgga tcgttgcact atctacttga acggcttcac ttaattcagt tgaagacact
     6601 tttttctttc cagcattatt taaaatcctt aaatagcgat aatacaaagg cagtctttta
     6661 gctgtcgcct ttggaataat atgatctttc acgaaatgac ctccacgtat tgtctaatta
     6721 catctttgat ttctccatca tagcagtatt tataggaaaa aatcaattcc attgctcaga
     6781 tattgtgaaa ttttattctt tattcattta tttatcattt ctgcaaatct cgcagtataa
     6841 tggaatttat ggtagactac taattgaaaa atagtttttt atttgaggag aattcacaaa
     6901 gtagaaaaga ggattccatg atattattac aagccaatca gattgctcgc cattttggtg
     6961 cagaagtctt atttgaaaac atacatttag aaatcagtac aggcgctcgt gtcgctttag
     7021 ttggcagaaa tggcgctgga aaatcgactt tattaaagat cattgctgga attgaagcac
     7081 ctgatgaagg aaagatcgct aaaaataaaa cagcgactct cggatacctc gctcaggaca
     7141 caggacttga ttccaatgaa acagtttgga acgagatgct gaaagctttt gaacaagtta
     7201 gacaaatgga agagcggatg cgcgagctag agattttcat tagcgaaggt acgccagaca
     7261 cacctgctta tagttcttat ttaaaagaat acgaccggct gcaacacgat ttttcagatc
     7321 gaaacggcta tggttatgaa aatgaaattc gttccgtatt gcatgggttt aaattcgatt
     7381 cttcatttta tgatcagaaa atcgatactt tatcaggcgg acagcgcaca cgtttagctt
     7441 tagctcgcat gcttttgcaa caccctgaca ttttgattct agatgaacca acgaaccatt
     7501 tagatatcga gacactttcc tggcttgaga attatctgca aagctatact ggtgcattat
     7561 tgattgtttc ccatgaccgt tattttttag ataaagtagt caatgaagtc tatgaattaa
     7621 gcagaagaaa aatgcgccat tacaaaggta actattcgaa atatctagat ttgaaagcag
     7681 aacagttagc aagtgaatgg aaggcttatg aaaagcagca aacagaaatc cataaattag
     7741 aagacttcgt tgctcggaac cttgttcgcg cctctaccac aaaacgagcg caaagccgca
     7801 gaaagacatt agaaaaaatg gaccggctgg atcgtcctca aggggatgaa aaacaagcaa
     7861 actttctatt cagtatagaa aagccttctg gaaatgtagt cttgcaaatc gaagatgccg
     7921 ctatcggata tgataatgtc accttatctg aaccaatcaa tctagatatc cgtcgtcaag
     7981 aagcgattgc tctagtaggt ccgaatggta tcgggaaatc gacgttgctg aaatcaatca
     8041 ttcaccagat cccttttatc cgtggaaaag aaacgctagg gacaaatgtc accatcggct
     8101 attacgatca aggtcaagct gatctgcata caaacaagac gatcctagca gaattgtggg
     8161 atgagcatcc taccactcca gaaaaagaga tccgaagtgc tttaggtgct ttcttatttt
     8221 ctggcgaaga tgtagaaaaa ccaatccctc tgctaagtgg cggtgaaaaa gcacgggttg
     8281 ctttagccaa gttagcgatg aacaaagaaa attttttgat tttggatgaa ccaacaaacc
     8341 atttagatat tgacaacaaa gaagtattag aaaatgcatt gatcgattat gacggcacac
     8401 tgctttttgt ttcccatgac cgatatttca tcaatcggat cgcgacaaaa gtcgtcgaac
     8461 tctctgaaaa tggcagcaaa gtctatctag gagactatga ttattattta gagaaaaagc
     8521 aggaagaaga agaaatcgcc gaattgttgg cacaagatga gccagaatct gctcctgctc
     8581 ccacgaacaa atctgattat taccaaagca aagaacagca aaagcttatc cgtagccttc
     8641 aacgaaaaat tactcaggtt gaagaagaaa tggcgcggat cgatgaatgg atcgatcagt
     8701 tggaacaaga aatggtcgcg ccagaaaacc tggaagatca cgtaaaactc aatgagctga
     8761 accaagattt agaagctgct agacaagatc aagaaacaaa attgacggaa tgggaagaac
     8821 taagcttgga attagaagaa tatgaaaatt aacgaaaaaa ccaaaacaga aaccattcat
     8881 gctgttttgg tttttctttt tttattttat caaacaaaaa ttccatattt acctttttca
     8941 ccaataagaa gaccaattac tagtcattct cactaaagct tctaaataat agtaatcacc
     9001 ccaaatacaa ggtgtattta ccccattatt tttgggctta ttataaacag actgtgttaa
     9061 aaagccatct tcttcttccg ttgtagcata tctttctttc aattgtcgca taattttcca
     9121 aacagcagac tggtataagt tcttttctgt tttgtctaat agacctgatt ctattaattc
     9181 taataatcca caaaccatga tcaccatact agatgtatct ttttcttctc tgctagaatc
     9241 atcaaagatc aaatcccaat aacaaatggt atcttctggc aaccgattca taaaataatt
     9301 ggctaatatt ttgcttaaat caaaatattt ttgttctttc aaataacgat aacttaatgc
     9361 aaaaccgtaa atcccccatg cttggcctct agcccaacag gaattatcac tataaccttg
     9421 atgcgtactt ccatatcttg gttttccatc tttgacatcc atataaaatg tatgataagt
     9481 tgaaccatct tctcttacta aataatctgc tgcattttga atatgagcct gagcagcttt
     9541 tttatatttt tgatttccag tttcttcact cgcccaatac aataatggaa gattcatgca
     9601 acaatccatg atcattctac cttgttgtga aggatcacta ggatctcccc aagcttgtat
     9661 aactcctgcc ttctcccaaa aacgctgaag taatgtttct gcagcatcaa ttgaagcttc
     9721 cttcattttt cgatttcccg ttattttata atctgctacg gttgataaag aatacaaaaa
     9781 accaagatcg tgatgattag tgataatttg acagtcaatc ctgtggataa aatctatatc
     9841 tgctttttca gctgtttgtt gaaataaagg atcacccgtc ttctcaaata acaaccaatt
     9901 aattcctgtc caaaatcctt ctgtccaacc aatattgtct cttaatggat aacgtccttt
     9961 tataggacac tcgcttggga attgatactg actgttactg atacgtttga ttaacctctc
    10021 cgtgtgtctg cgacaacgct cgatctcatt attccattct ttttccatta attctttata
    10081 acattcattt atccattttt ctggatgtat tatctcttca aacatcactt tatcaagcat
    10141 ttccccacca gcctttagca taattttagt ctttttttat tgtttttatt ttggatagtt
    10201 agcccggttg tcccatcaaa atcaattttg ggacgattgt tccatacttc taaactataa
    10261 tcccgatctt gatgagcaac cactcctgtt ccgagccaat gggttccttt tccaaatgta
    10321 ccctctaatc caggaataaa agtgttttcc catgtcatca agtttgtatt gggcaaaccc
    10381 ataatagctg tggattttcg tttaatagta tcgtcactta aacagataat acctgaaaag
    10441 ccagcttcac tcaccctata gttttccttg tcttcttcta tgattattcc ttctttctca
    10501 ggagaacatt caatagcaaa ccctgtctca tatagaacat attctgtatc taattctact
    10561 ttgtgtacgc gaatctgcca gccttcaaaa ggaataagcc aagttctgat ttttgcctgt
    10621 aaagtaggtt cccatgtaga ggcaagtacc tctttcttac atacaatgtc tcgtacatta
    10681 tttcgaacga tgaactgtcc ataggttttg atgctctcat tcttaaaact ggtcgtgata
    10741 tatgtgtccg ctcgctgaat ccctagcatt gaatccattg ccatttctgc tttcaattga
    10801 ttcgctcgcg gtatcgaaaa accaaattga ctagaataag caaattttaa atatttttca
    10861 ttggtcaatg tatggaaatt tggccctaat tgtcccgcat tcaaaaaaaa tgtatgtcca
    10921 ttatcatgtt gtatgagcaa attggcttct gaaagaagtt tagttccttc acgaataggc
    10981 ataggcattt ctttagccgt ccaatactca tggtcttcag gtagcgccaa caataaaaaa
    11041 atcttattca gccataatgg agataacgta ctattatagg gttcagtcaa tgtcaattga
    11101 gggtaggtat aaccaatatt caggatacct ttttcatcaa atatttcttt ttgcatccac
    11161 catcttatat tccgattgac taaccccttg atttcaccta atttaaaagg caaaagatcg
    11221 gcccacaaca tagctgacca aaagcttgaa actgcaaaac gataaatcat gcttcgacca
    11281 aaaggcacat ttgcaccatc ttccgcaaaa aaatgaatat agtctctaga aaaatcaata
    11341 gctcggttct tatatatctg gctaatgggc ttttctggtt ctagtacgct ataaatcaaa
    11401 ccataataat gaaatgcaaa aggattataa taatcttggc gacctcttga actgtctcga
    11461 taccagccat tttgaagata acaattttct atattatcaa atgcttcttt cagtgctgat
    11521 tctttaactg gataaccaaa tttttggata attgttccga caattatctt aaaaaattgc
    11581 caattaccat ctgcacattc atattcaaaa atttgcgaga accaatgaat aatctggctt
    11641 ttttctcctg tatctaattt tttccataat aattgttcat gatgatataa cgctaataac
    11701 attgcgggca tttctaccat acgctggtct ctttcatgta tttctcccca ataatcggga
    11761 tgggaaggat ctgttccagc ttttattcct gatagaatta atttcaacca tcgttcatct
    11821 aattggtcaa ttgcgggccc cgctccccat aaaacccgag agaatccttc cattcctgca
    11881 attttatttc catatcctac cccttgatgt atataatcaa tttgtgcgcc acttgctcga
    11941 taaaacggct ggagtggttg atttatcttt ttaaataagt caaagtaatc ctgtctggtc
    12001 cttaatagtg gtactggatg tttcattgta ttttctcctc tttttactac caaatagcta
    12061 attccgactg ttctaatcga agtaagcctt caataaaaaa ataatcagaa tagatcagtg
    12121 gcacattata agtcagttct ctatggtatg cttctgctcc gcctccaatg atcccatctt
    12181 catccatatt ccaatttgca tagtgctttt ctgtcttttg gagtatattg atagcagctt
    12241 catgatacat ttttgatcgt acagaggaaa catatcctgc taattccaga agaccgcatg
    12301 cagcgcatac tccagcactg gtatcaatat tcgaatcttt tacagcaaaa tcaatgagag
    12361 gaatataacc agttttttcg attttctgaa taaaatttac tgctatcttt tctgctatag
    12421 ctagatagta cggatttttt gtgtaacgat aacttaacgc gaatccatat aaagcccatg
    12481 catttcctct gctccaacaa gaattctcat tgtatccttg cccgccaagt aaccgaatat
    12541 atttccctga ttctgcatca aattctgcga tatgcccggt tgatccatct ttcctcacta
    12601 gatacttagc tgttgtatct gcatgctgcc gagcaatttt agagaacctc ggatcaccag
    12661 tttccctcga agcccaatac agtaatggta aattcatcat actatcaata atcatccaac
    12721 caggtttatg gtcattccat gctactaaat agttccctga aatattatag cgtccagcaa
    12781 gtaccgtagc tgcatgtaac cctagttgtc gactatgttt gttttgagac tgttgataat
    12841 gtgcaatagc cgatggtaag aataaaaaac ctacatcatg atgaacgtct gaaaaatggt
    12901 acagggcaga ttctaataat tgttcttgtt gtattgctgc ctctttgaac ttttctcgtt
    12961 ttgtcttgtg aaagagctgc cataaaatac cactccaaaa accattggtc caccagtcga
    13021 ttcctctgtc ctttattacg tctgtatatt ttccgttgta agaaatatat ggaattttat
    13081 tccccattct gtctaattcc gcatctatct tttcagaagc tacattcaaa atttttcttg
    13141 accattcctt cgtctgttca ttcatggata tcccgccatt cttttaattt ttatgaatgt
    13201 ggagttgctc ctagaacgaa ctagatggtt aacattcgtt atttttttta cttatttgca
    13261 gctccaatgt cattctcctt aaatttagac tgcgcccaca cataaatcaa gaccattggg
    13321 atcactgaag ttgctaaaat tagcataatt gcgccataat taattggctc acctgtacta
    13381 gaaaccgtca tatttttgat aattaaggga accgtatatt tatctttatc tgaaagcatg
    13441 actaatggaa atagatattg attccaactt cccataaaga taagtaaacc actagtaata
    13501 atcgtaggtg ataaaatcgg tacagcgatt ttcgtaaaaa tcacccactc attggcacca
    13561 tcgattctgg cagcctccat cagtgctgta ggaaattgac tgctatattg tcgcatcata
    13621 aacactgaag ttgtactggc aatcgaaggg atgatcacac ctgcataagt attcgataat
    13681 cctaaacgac tcataatgta aaataaagga atcaatgtgg taaaaaaagg caccatccta
    13741 gatagcataa ctgtattgaa caacagttta ttccccataa aacgatactt tgctaatgca
    13801 taacccgcca ttgtagaact ggctgtagct aaaactgtac tgattacagc aataacaatc
    13861 gaattccaaa taatcaaggg ataattatat ctcgccatga tggcttgcca gttttttaat
    13921 agcatatccc caaacttgaa attgattccg ccacttaaaa tttcttgatt actttgcgtt
    13981 gaagctacaa tcatatataa atatggcatc aaaaatatta aggcaccaaa cacaataatg
    14041 acgtacgtaa aaattctgct ttgtatcttt tgtttagtca tttttatcac ctccgaattt
    14101 aaattgaata gccgataata taacagatac aaacaccatc gtccaggcaa tagcagaggc
    14161 ttttccaaaa tcaaccattt caaaacttgt tttatataaa ttgactccta cgaccaaagc
    14221 tgaattttct ggtccattgg aagtcccaaa gaatatattt gggatattga aattacccaa
    14281 gccattgata gtggctaaaa ctgtggccat taaaatgatt ggctttagca tggggatcgt
    14341 taataaaaag aattgccgaa atttcgatgc tccatctatt tcagcacttt cgtatatctc
    14401 tttagaaatc ccttgaagac cagctaaaaa taaaatagtg ttatatccta cactaagcca
    14461 aatcatcgtg atgatcattg atattcgtat ccaaaaagga tttgttaacc actgtacacc
    14521 gttaattcct agcgcattca aaaatccatt gaataaccca gtcggattga aaaaagtcat
    14581 gaatacggaa gctacagcca ccgttgatgt aatggttgga atataataga cagttctaaa
    14641 aaatcctttt ccttttaact gtgctgaatt caataaaaca gcaaagtaaa tagctaatag
    14701 catcgttatt ggaattgatc ctgccatgat aatcaaaaca ttcgtgtaag atttaataaa
    14761 caatggatct cggaagatag ctagataatt gtcaaatcca acaaattcaa atgagtagcc
    14821 aattttcata aaactaaaag caaacgagat aatgatggga atcaaaatcg tcacaataaa
    14881 gaaaataaca aaaggtgtga ccaatccata agcacttttt ttatgagacg tatctgttgc
    14941 ttttttcttg ctgttcttta ttgtcttgct tggtaacgaa gattgtatca tcgttaagtc
    15001 ctcctaatat tttttaatca ccagttaatt ttacgccgta cttatgagca aaattttctg
    15061 cttcttttag atatgaattt tcaaaatcac ctgtttgcca atagttatat gcagcggtag
    15121 tcaaataaga cgtagcatct gaataagtgg ctggtaaaac agtatttgat gtattttcat
    15181 tgattttatt caatagttca tagtattttt gtcctcctta gtagtcaaac tttttctcag
    15241 ctgcttctgt ttgataggcc aatgagttag atgtagccac gcccagctcc agtgcgttgg
    15301 ctaagcattc ttcatctgtc agcataaatg taagaaattg ttgtgccact tgtggatttt
    15361 tggcatcttt agggacataa aaacttgatc ctcctgatac tgcacctttt cctggatttt
    15421 cttttgaaaa aggaatcagt ggagccatgc gccatttgtc agatgcatca ggaaaattca
    15481 aagacatatt cgtagcatac cagccgccag caacaaacat tgcagattct tgaaatgctt
    15541 tctcagcatc ttgtgcaccg tagaaatgta caatatctga gtcgatcatt tctttgatta
    15601 actttgcagc atgtttcgct tctttagtat tcagacggat atttccttct tcatcaagca
    15661 aagattgatt ctgctgggct agcatgttga taaacaattc ttggtcccca gattctggca
    15721 aggcaattcc aaaactacca gttttttctt ttaatgcttt tgccacctgg atatattctt
    15781 cccaagaact gatttcttca aaatctacac ctatttcagc aaattttttt ggttgataat
    15841 aacaaagtgc tggggtaaaa tcactagccc aaggaataat cgtttcatta ctcgtttgtt
    15901 gacgcaaaat attcaatttt tgatcataaa actgatcgcc atatttgtca taaaatccat
    15961 attcatcagc actataaaaa gcatcttgaa atttttccag cacacctgct gctgaagtgt
    16021 ctgccatgga gataatatcc ggcatatctt cgtgtgttac caattttggg gtgatcattt
    16081 gtactgttgc tgttcccgaa gctacattca caatttttaa gtcaatattt gctccagttt
    16141 tgcggttata gatttttgca gcattttcaa catagtttgt cagtttgccg tttgcgacat
    16201 atacttctaa tgtagtggct ttttttatca ttggatctgc gatgtttgct gttacttctt
    16261 cgttagtttt tccacatgca cttaagctga ataatagggt aatgtttagc aaagctatca
    16321 ccaatttgta tctttttttc atgtctatcc cttctttctt ttcaagttct tatataaaac
    16381 gctttcactt atgtatatta acggaattag taacacaaat atttatataa aaaaatattt
    16441 tacttgtcgt tttggctttt catttttgtt ataatagtca ttaattataa atagaaagct
    16501 ttttatataa aaagagaaag gaaagtggtt cttttgttaa caaacaacca atcctccaat
    16561 cttgtcaaaa aaacactaac ctattccata gatacccatc ttcatttatc tgctgctccc
    16621 tattttcgac tactaataaa tgaaagcgct aatttatatg ctgaatttgc acataaatct
    16681 atagaactag ataatccttc ccttttattc attgataaac agcaagaagt ccaaatcttt
    16741 tctactcaaa aggaaacaca aacttttatc attatccagt tttattttga tgagcaatta
    16801 ttagcacata ttacgggaca atctttttct atagatcaag aaataacatc tatcattact
    16861 tttctgaaaa atgaacagtt caaaattgat tatctaaaac atagtaagcc agctatttcc
    16921 tatgatcagg aaatcaattc ttttttcata ctatgccaac agatgagcat tcatttgctt
    16981 tccttattag ttacttattg tctgcaaaaa attcaattgt cttccttacc aaggcattat
    17041 caacaggaac ttgctttatt gacaatacct tcaaatgaga aagtcattcc taaaaaaaag
    17101 catattcttt attcagatga attagttgat ccaatcatac gatatttaga agaacatctt
    17161 tcagaaaatt ttttgcttga acaattagcc caaatttttt ttatcagtcc agccagctta
    17221 aaaaaacatt ttagaaaagt gaatggaaaa agtgttatta gctattttaa agaactaaaa
    17281 atggaaaaag caaaacgtct cgtgctagaa aatgaattaa cctatacaga aattgcagat
    17341 atattaggat ttcattcgat ccatcatttt tcttcagctt ttaaaaaata tacagggtta
    17401 tccccttcaa agtattatct atccttaaaa tagaaaaaaa tttttaggtt ttatccacaa
    17461 attatttctc tatattttct gtctattcaa aataaacaat aattttccgg cacaattatt
    17521 tgctgttttt taccataaca tagtacgatc aacgtagaaa aggagatgtt tttatgaaag
    17581 ttgttgtcat tggcggtgta gctggcggtc cttcatttgc cactcgtttc cgtcgattga
    17641 atgaagcgca cgaaatcatt atctatgaac gcggagaaaa tatttcttat gcaagttgtg
    17701 ctttgcctta ttatttagga ggtgtgatca ctgaccgtga ctcgctgatc gaacgtacac
    17761 cagaaatatt gaaaacaaaa aacaacatcg atgtctttac taaacacgaa gtaacagcaa
    17821 tcgatccttc tactaagcga ttaacagtta aagacctatc cacaaatgaa gaaataaaaa
    17881 cagattacga taagttgatc atctcttctg gtgctagacc agattatccg gatattcccg
    17941 gagtttttga agcagaaaac ggctttgtcc tccgtagtgt gacggatgcg gatcgaatca
    18001 aatcgttcct tgaagaaaaa aatccacaac atgtcgtcat tcttggtgca ggtgttatgg
    18061 gtctggaatt agctgagaat ttcaagcatc gcggcttaga cgtgacttta atcgatcaat
    18121 tgccacaagt cgctttccct tatgatccag aaattgctaa tttagtttat gacaaattgc
    18181 tgaaagaagg attagccgtt catttagaaa caagagttac tgagatccgt gataaaggtc
    18241 gagaaatagt attatcagat ggctccgtcc tttctgctga tatgctaatt tttgctgttg
    18301 gtgtttctcc gaagaatgaa gtggtgaaag caaccggcat acaattatca gatacaggtc
    18361 agatcattgt tgatgaccag ttacaaacca atctgccgga catctatgcg attggcgata
    18421 ttatcgaaac gactagtgta gtgactggtc agccgatcca aagtatgctt tccagtgcgg
    18481 ccaatcgtca aggacatatg ctggcagata ttttaaatgg tacgcctatg cgctatcgcg
    18541 gatatattgg tgcaggtgtc gcaaaaatct ttgatcatac agcaagttat gcgggaatga
    18601 cagaacatga attaaaagca tcaggcataa caaattataa aactgttttt atcactccct
    18661 ttgaccatgc ctatttctat ccagaagcta caagattaaa tctaaagctg atttttgatg
    18721 cagatagcgg tcgtatttta ggtggacaag catttggaga aaaaggtgtc gataaacgga
    18781 tgggagaact ttctgtagca atcaccggaa acttgacagt ctttgatttg cctgatttgg
    18841 agttgcctta ctctccaccg tattctacta cccgtgaccc gttgaatata gctggttatg
    18901 tcgcaatcaa tcaaatgacg aatatcgtag aaacgatcaa agcaagtgat atacccgaaa
    18961 acgatttgaa agaagcgttc tttttagata tacgtgaacc taataaagca ccttctggca
    19021 gtatctcggc aacaaaaaat attccaatga atgagttacg agatcgaatc aatgaaatcc
    19081 caaaagataa aaaagtttac attactttca gaagaggatt gaatacttat acttctgccc
    19141 gaatcttggc aggtttgggt atcaaagcga ttttgattga agaataaaaa aacgaccgcg
    19201 gtgggtagcc gcggtctagg agttattttt acttgaggag taaaaatgaa aaagtatata
    19261 gggttgttgg ttggtatgta tatagaatag aagataaatg tgaagaaatt gtgcattatt
    19321 tcttcacatt tattaggtta tagtttaaga aagtttaaac attgcgcaat ttaattattt
    19381 tagctgtttc ttttcataaa aaaacatgtt gtgactctga gacaaatcat agtccatagt
    19441 gatgtctcta gagtaactac gcctaatatt acgaggcatc atattaatct atcagcacta
    19501 gtacctaaac tagcatccct ttcgaatatt ttttcttata taataaagtt tgctttcact
    19561 cctctaatct caccaaatcg actggcttgc gttgtgtgat actaggaact gattggttta
    19621 gaacgacttt tctattgtaa tcatattttt cgccatcctc gttgtcttct aatccttcaa
    19681 tgaatgatcc ctaatgtgtg tattaaataa tttaattttt ttgttcctat ttatttaaca
//
LOCUS       DACGLW010000053        19508 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.055, whole genome shotgun sequence.
ACCESSION   DACGLW010000053 DACGLW010000000
VERSION     DACGLW010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19508
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.055"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            complement(460..1089)
                     /gene="udk"
                     /locus_tag="IXT53_001884"
     CDS             complement(460..1089)
                     /gene="udk"
                     /locus_tag="IXT53_001884"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HAQ4905689.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1173..1868)
                     /locus_tag="IXT53_001885"
     CDS             complement(1173..1868)
                     /locus_tag="IXT53_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="noncanonical pyrimidine nucleotidase, YjjG
                     family"
                     /protein_id="HAQ4905690.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1881..2597)
                     /locus_tag="IXT53_001886"
     CDS             complement(1881..2597)
                     /locus_tag="IXT53_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HAQ4905691.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2927..3532
                     /locus_tag="IXT53_001887"
     CDS             2927..3532
                     /locus_tag="IXT53_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HAQ4905692.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEESTGEGGEPEVIGSTEE"
     gene            3857..4180
                     /locus_tag="IXT53_001888"
     CDS             3857..4180
                     /locus_tag="IXT53_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HAQ4905693.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4362..5732
                     /locus_tag="IXT53_001889"
     CDS             4362..5732
                     /locus_tag="IXT53_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HAQ4905694.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5862..7163)
                     /locus_tag="IXT53_001890"
     CDS             complement(5862..7163)
                     /locus_tag="IXT53_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HAQ4905695.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKIE"
     gene            complement(7393..9384)
                     /locus_tag="IXT53_001891"
     CDS             complement(7393..9384)
                     /locus_tag="IXT53_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HAQ4905696.1"
                     /translation="MATTHNTKKISMAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGL
                     SGINPDFVIGSVSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLII
                     PAMIGGAALLADGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFM
                     VQRFGTDIVGKAFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKL
                     GLFILGNIFLATTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQN
                     PEAQQIKNLNPFFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPR
                     LKIMYPGNSIGQMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALL
                     YFYLIQNGHSRWLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWE
                     QGNIIRESSTEDVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSI
                     LDKRPKRARVYWFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQ
                     VIHDLMKQGRLPKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAI
                     KKIATSPETWFGLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9528..9794)
                     /locus_tag="IXT53_001892"
     CDS             complement(9528..9794)
                     /locus_tag="IXT53_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ4905697.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10304..12430)
                     /gene="pnp"
                     /locus_tag="IXT53_001893"
     CDS             complement(10304..12430)
                     /gene="pnp"
                     /locus_tag="IXT53_001893"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HAQ4905698.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12618..12887)
                     /gene="rpsO"
                     /locus_tag="IXT53_001894"
     CDS             complement(12618..12887)
                     /gene="rpsO"
                     /locus_tag="IXT53_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HAQ4905699.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13138..13701
                     /locus_tag="IXT53_001895"
     CDS             13138..13701
                     /locus_tag="IXT53_001895"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HAQ4905700.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(13954..14475)
                     /locus_tag="IXT53_001896"
     CDS             complement(13954..14475)
                     /locus_tag="IXT53_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HAQ4905701.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14478..15320)
                     /locus_tag="IXT53_001897"
     CDS             complement(14478..15320)
                     /locus_tag="IXT53_001897"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HAQ4905702.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15313..16038)
                     /locus_tag="IXT53_001898"
     CDS             complement(15313..16038)
                     /locus_tag="IXT53_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HAQ4905703.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16214..16510
                     /locus_tag="IXT53_001899"
     CDS             16214..16510
                     /locus_tag="IXT53_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905704.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17078..18670
                     /locus_tag="IXT53_001900"
     CDS             17078..18670
                     /locus_tag="IXT53_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750719.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ4905705.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18683..19423
                     /locus_tag="IXT53_001901"
     CDS             18683..19423
                     /locus_tag="IXT53_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ4905706.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
BASE COUNT         5581 a         4002 c         3702 g         6223 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaaa cagttggctg gtgactgttc aaaaagcaga atccttgctt
      421 ttcaaacggt cgcagtccgt tcaactgttt cgctgcgcac tatttatggt ttaagaaact
      481 agctactttt gttgtaatca aatcaatagc aacgtggttt tctccgcctt ctggaacaat
      541 gatatccgca tagcgcttcg ttggttcgat aaattgatga tacatcggtt taacaactgt
      601 caaatattgt tcgatcacgg agtccaatgt gcgtccccgt tcttccatat cccgtttgat
      661 acgacgaatg atacgaatgt catcatctgt atctacgtat actttgatat ccatcaagtc
      721 acgcagacgt tcgtcttcta aaatcaggat tccttctaga atgatcactt cttttggttc
      781 ctgaatgatc gttgcctgac ttcttgtatg cgctacatag tcatacacgg gtttttcaat
      841 ggctttatag ttcagtaagt cgcctacatg ctgaatcagc agatcattat caaaggcaaa
      901 tgggtgatcg taatttgtgt tcaatcgttc ttcaaaactc aaatggctct gatccttgta
      961 ataagaatct tgttcaagca tcatgatgga atggtccggg aagttgttga aaatcgcacg
     1021 gctgacactt gtctttccgc ttccggatcc acccgtcaca ccaatgataa ttggcttgct
     1081 ttttgtcatt gactaaaacc tctttcgtat ccgttttttc attctaatct attatagcga
     1141 aaaaacacga aaatgctata tttttctgaa aattaatcct tttcaatttc caataatgaa
     1201 taaagttcct ctaatgaatc gattcgataa gtaggctgga tttcttcgat ttctggtaat
     1261 ttatttctat tgaaccaaat cgtatcgatc cctgccatat ttcctccttt gatatcagaa
     1321 gtcaaagaat caccaatgat cacagtttgc tttttatcaa aattaggaat gtgggaaaag
     1381 acatgatcaa aaaattcttt catcggcttt tgcgctccga cagcttctga gacaaagata
     1441 tccttgaagt aatcaagcat tcctgcatct gttaatcgcc gatgttgcgt gtgcgacaca
     1501 ccgttcgtca caatataaag atcagcatgc ggatggacct ttttcaggat ttctagactt
     1561 cctggaacaa gatcatgacc ttggttcaag tagtggcggt agcgtttttc catttgatca
     1621 ctatctactg ttttcccaaa ttgctggaaa agtaacccaa aacgactgcc tattatttct
     1681 tctcgtgata gtctgccttt ttcaaactcc tgccataaat gatgattcaa agccttatag
     1741 ctggtttcga tttctggtgt caatgtgatt ccttgttctg caaacaaagc acttaaagca
     1801 ttggcttcag ttaattgaaa atctaacaat gtatcatcta catcgaacaa taaagtttta
     1861 tatttcatct ttttgacctc ctatttaaaa taaatactta actttttcat ctctgctaat
     1921 gtcaacgatc gatactcgcc tactcctagc tgttcatcta attccaatgg tcccatgctt
     1981 agccgtttca atgcggtcac tttctttcct tgggctaaga acatcttttt gacttgatgg
     2041 aattttcctt cttggatcgt caataaaacc gtactttggt gcttgtctgc tttgagtatt
     2101 tttaaatcag ccggcttaca ttgataccca tcagcgaata cgattccatt ttcaaatgct
     2161 gttacgtctg ccggtgtcac cctttcgttc acaactactt gataagtctt tgaaaccttt
     2221 ttttctggaa gtagcatttc atatcccaat tgtccattcg aagtgatcag caatagtcct
     2281 gttgtatctc tatctagtct tcccactgga tataatggtt ggaaacgatc ggcacttgca
     2341 atcagatcga ttactgtcgt cttttctgga tctgatacag ctgtaacagc tttttcaggt
     2401 ttattgagaa gataatagga ctcatttgtt tctaagtgtt cacctgctac ttcgatttga
     2461 tgcagtaaac tatctacatt tcgattttgt cgaaattcga ctgtgccatc tatcatcact
     2521 tgcttcaaag caaacagccg cttcatttcc ttgcgtgtcg tttctaattt ttcttctatt
     2581 aatttatcta aacgcatgct gtttcctttc ttattactac taattaatta taccttgaat
     2641 attcttgctt gcaaatacat catcatagtt attccttgac ttaattaaca gttcacgtta
     2701 cggttaaata gtaaagtgaa ttggatttct catacacagc gtaatgacct ggtggagaaa
     2761 agttgaatcc tgactttcta aggggagatg cgcgcatctc tccttttgcg cttttttaag
     2821 cgccctgcct tcgatgatgt gcacgagatt agcctttaga attcacttaa ataaaatctg
     2881 tccgttacca aagcagacag tcacatttta aggaggaatt ttggttatgt cagtatcatt
     2941 agaagtaagt aaaagagaag tacgtccccg ttcattaaga aacaaacttc gtcacgaagg
     3001 gaaagttcca gctgttgttt atgggtataa agtagaaagt acccctatct cattcgatgg
     3061 tttgacacta agcaaattgt tgcgtgaaca tggagcgaac acagtcatca cgatggacat
     3121 cgatggacaa aaagtcaata cattggttca caagatccaa acgaacactt ttaccaatca
     3181 tttcgaacat gcagaatttc tatctgtaaa catgtcggaa gaaacagaag tcgaaacaga
     3241 agtcgttctt gttggcgaat cagtgggtgc taaatctggt ggagtgttag ctcaaaactt
     3301 gtatacagta gtcgtatctg ctacaccaga caaattgcct gaacgaattg aagttgacgt
     3361 gacaaacttg gaattaggtg attctattac agtagccgat cttccaaaaa acgacgatta
     3421 caaattcgtg accgacagtg aagagcaaat cgctgctgta gtcgctcaaa gtgaagaaga
     3481 aagcacaggt gaaggtggcg aaccagaagt gatcggttca acagaagaat aatactgaaa
     3541 caatcaggtg gtgttagaag cggcgggtca gcagctttta acacgccttt tttatttcta
     3601 atcgtttatt ttcatatggg gcttccactt ttgtaaatgg ctttacaaac gaaaaatcct
     3661 aatttaccaa gcttttcctc gcatactttt atcgtacctt tataaaaatt tatactttga
     3721 aaaaaagtat tcaattattg ttgcattttt cctgattctt tattagaatg aaccctcgtt
     3781 gaagtaagta tttctagttt atcctaggat taattataaa aataaaacat tcgttgattc
     3841 gaaggaggtt aaacagatgt ttgaaagttt tgatagcagc cgcaaccgtt acgcttcagt
     3901 tggtgtcgta tcaagtttgc cagatgaatt gattgacagt atatggttta ttattgacct
     3961 agatcttaag ggagtcattc cattagaaaa tatattaacc tttgatttga tcgaaaatcg
     4021 tggaaaagtg acgatgcatt tttctcaagc aggaagcaat gtggaaatgg atattgattt
     4081 gccgtttgcc tattcgaacc aatttcctgc tcaagtatac gcctatgatg acggtacgag
     4141 agaaacgatc cttttaccaa gtgagattag acaatattaa ggtgatagat aaacgtggat
     4201 gtcccttatt ttacagacca ggccaagttg ttgattcttt gactcaattc ttggcttctt
     4261 tttatggagt ttcataaatt atcattgatt tctaattggt attctcagga taaacgctat
     4321 atactactaa ctaaatttta aagatataag gagtttaaaa gatgcgcgat ttaacaaaag
     4381 gaagccccgc taaactgatt ttgatgttca ctgttccttt actgatcgga aatgtttttc
     4441 agcagttcta caatatgatc gatatgatca tcgtgggtca gacgctaggt aaaaatgcgc
     4501 tggcagcggt tggtgcaact ggaagcctga catttttgat cattggcttt gcccaaggtt
     4561 taacagctgg tttagccatt attaccgcac aacgttacgg tgcaaaagat tatcggggtc
     4621 tgaagaaaag ttttgctgct agtgttgtca ttagtcttgt cgtcactgtg attttaactg
     4681 tcctaagtct cctgtttatt cgtccgatgc ttcaattgat gcaaactcct cctgaaatac
     4741 ttgatcaagc acaaacattt atttcaatta ttttattagg gatctttgca tcagtcagtt
     4801 tcaatttgct ttctaatgtc attcgcgcac ttggtgacag ccgcacacca ctattctttc
     4861 tgatcattgc ggtcatcatc aacgtcgtac ttgatttgat ctttattatc tattttggca
     4921 tgggtgtgga aggtgcagct attgctacag taatcgcaca agtttcttcc agcgtactgt
     4981 gtctagtgta tatcaagaaa aaaatgcctt tgttacagtt acgcaaaaaa gatttcaaat
     5041 ttgaaaaaga agagtttgct gtacacctaa atgctgcttt gccaatggcg tttcaatctt
     5101 cgatcatcgc tgttggtgct atcgtgttac aagcagcact aaatagtcta gggacagatg
     5161 tcgtagctgc tcaagccgct gctggtcgta tcgaccaatt tgcaaatcag ccgatgatgt
     5221 catttggtat tgcaatggca actttcactg cacaaaacta tggtgcgaaa gaatatggcc
     5281 gaatcttgca aggggtacgt caaactttac tgatgagtgt cggatttagt ctcttagcag
     5341 gtgctatcgt cattttcttc ggtcatttct ttgttcaatt gtttgttagc ccatcagaaa
     5401 cacgtgtatt tgaattagca caaacctact tcaatatcaa tgggtctctt tactggatct
     5461 tagcagtcct gttcatttta cgctacacac ttcaagggct tggacaaagc aagatcccta
     5521 cgatcgccgg tatgatggaa ttgcttatgc gttcttttgc agctatcgtt cttacaggaa
     5581 tgtggggata cccaggagca gcagcagctt ctcctttagc ttggatcggt tctgttgccg
     5641 tgttgcttta ctcttatatg cgttcgatga aacaattgaa acacatgggt gaagaggaac
     5701 atttcacatt agaacatgca gaagcaagat agccttgatt aaaagaaatc gttgcggcta
     5761 aaaatgaaaa cttttattga aaatatcttc ttcgtttacc gaaaacaggt tttttagata
     5821 acaaaaaggc tgtgacaaaa aatgtcacag ccttttttct tttattctat ttttctttcc
     5881 gtggactcta ctttgaaata atcgatatgt gcatgctggt attctaatac cgtgaaacgg
     5941 aatgatgcaa tctcaatgat tgttcctgct tttatttctt tatatcggct agaagcaaag
     6001 cccgcaagtg tagtaaaatt cgtttcttca aactctggaa tctctacatg aaaataacgc
     6061 tggaaatcat ctaatgttag tttgccggag atgatataag agccatctga ctgttccgct
     6121 atcaatgctt cgtccaaatc tgtctcatct cggatttccc cgacgatttc ttctaaaata
     6181 tcttcaatcg tcactaagcc gcttgtccct ccgtattcat cttttaagat agcaatatgc
     6241 tttccctttt ttcgcatgat cgtcaataat ttttttatag ggatcgtttc agttgttacg
     6301 atcggctctt caatcaactg ttctactgtc agctggtcat ctttataaga ttgcttgatc
     6361 aaatctggca aggtgacata acctaaaaca ttgtcttttg attctaagat aaccggatag
     6421 cgagtatgtc cttgttcaat cgaagctttt atcgcttctt tgacggtttc tgaagcttcg
     6481 aatacttgca tggacgtacg gtctacttgt atatcttttg ccaaggtatc atcaaattca
     6541 aacacgtttt ccaaataatg atattcttct ttggtcaact ctcctttttc ataagaattc
     6601 aatgcgacta atgtcagctc ttcttgagac aatgtctctt ctccttcaga tacgagacgc
     6661 acacctaata gcttccctag ccctgcagct gaatgattca atacccaaat aaaaggaaac
     6721 attactttat aaaaataatg aagtggttta actacgaaca agacgacctt ctccgtattg
     6781 acgatactat agctcttagg aagcagttcg ccaataacca catcgatata cgtaactaaa
     6841 ataaatccca gcgccacaga gattgtactg ctaatagccg aactaaaagg tagtttaccg
     6901 atcactggtt ctagtaaagc cgcgacagat ccttcaccga cccacccgat gatcaaaccg
     6961 gttagcgtaa ttcctaattg actagctgat aaatagttat ctaaatggtg tgtaacatga
     7021 agagctagct tggcgttctt tacattttcc ttttctagtt gttccaatct tgatcgtctg
     7081 attttcacta aagcaaattc acttgctaca aaaaatgaag taattgcaat catcaaaaat
     7141 aaaataatga aattgataac catattctcc cgcctttctc tttttctatt gtaccctttt
     7201 tctacaaaaa gattgaaaat aatgcgtatt catatttagg aataaaaaag gagatgcggc
     7261 atttttcagc cacatctcct tatcacttca aaatcattgc gttacttgtt gctaaacagc
     7321 tttttcatga cctctgataa acggtgttca agtagctgac attcgacatt agttcaggtc
     7381 aaagcgctac tttcacaacc gctctttcaa ccatgttttc cgagtatttc caatgataag
     7441 cggaacagtt tcatgtttta cttcgctgta ctctagccca aaccatgttt ctggcgaggt
     7501 agcgattttt ttgattgcca gttttgcctg catgatttga cgttgccatt ttcctagagt
     7561 tgtcacattg gaaactactt catcaatcag gacaaattgg aaatcgccaa cttctcgccc
     7621 tggtgtcaga gagtatcttt gtggctgttt tggcaaacgg ccttgtttca tcaaatcatg
     7681 gatgacttgg cgaacatata cgttgatttc ctgttggacc ctgaaaccta agaacagatt
     7741 cacttggaca ataaaatcag tgtccatcat atctacttga tattctttcg tatatggctc
     7801 atccgtcact tccacatgaa caaaccaata gactcgtgct cttttaggcc gtttatctaa
     7861 aatcgaatag atgaactgtc ggccaacttt atcttcagcc atatcaggaa ccaagaagac
     7921 cacatttgtc tgatagattg gtaaagaagt atcatcacgt aattctctaa gctgcgggat
     7981 ataatctctc aaagacacat cctcggttga cgattcgcga ataatgttcc cttgttccca
     8041 aataaacatc acgactaaga tgatcaaacc aatcagaacg gctacaaaac cgcctcggaa
     8101 gaatttgaca acacttgaga taaagaaaac gacttctatc gcaccgaaga aaaaggtaac
     8161 taaatatgca agccaacgag aatgcccatt ttggataaga taaaagtaaa gcagtgctgt
     8221 tgtcatcaac atcgtcacag tgatcgatag accgtaagca gcttccatat gatgagacgt
     8281 tcggaatgtg acaaccacta gagaacaggc gatccataaa atcagattga ctgctgggat
     8341 atacatttgc ccgatactgt tcccaggata catgatcttt aaccgaggca gcagtttcaa
     8401 cttgattgct tctgaaacta gcgtaaacga acctgtaatc aatgcttgcg aagcaatgac
     8461 tgcagcgact gttgcaaaag caacgccgat cacgatccaa ctttgaggca tcatttggaa
     8521 gaatggattc aggttcttga tttgctgtgc ttcagggttt tgataaacat tcaacaacca
     8581 cgcagcctgc ccaaaataat tgataaccaa acaaattttg acgtaaggcc agctcagacg
     8641 aatatttcgt ttgccgacat gtccaagatc ggaataaagc gcctctgcac ctgtcgtagc
     8701 caggaaaata tttccaagaa tgaacaagcc taatttgtta tctgcactaa aaagcagctg
     8761 gatagcataa taaggattca gtgcacggat aactgtccag tcttgactga agttcattaa
     8821 tcccattacg cctaaaaaag taaaccaagc gaacatgatt ggtccgaatg cttttccgac
     8881 aatatctgta ccaaagcgtt ggaccatgaa taaaacaaaa ataataacta aagtgataat
     8941 gacgatcacg ttctggttat taccgaaccg gtcaaaaaac tgcggaattc ctcgcaagcc
     9001 ttcaatcgca gtcgttaccg tcacagcagg ggtcaatacc ccatctgcta gtaaggcagc
     9061 tcctccgatc attgcaggaa tgatcaaata tttcccacct tttcggacaa gtgtgtagag
     9121 tgagaaaatt ccaccttcac catggttatc tgcattcaat gcaatcacga catatttgat
     9181 tgttgtcagt agagtcaatg tccaaaaaat caatgagact gatccgataa caaaatcagg
     9241 attgattcct gacaatccgc cattatcttc aacaatcgat ttcataacgt aaagcggact
     9301 tgtcccgatg tctccgtaga cgactcccat cgctacaagc aggccagcca ttgatatctt
     9361 tttggtattg tgcgttgttg ccacgttttt ccctcatttc ttgttatatg gatatccttt
     9421 aggtaagctt gaatgataga tgatattctt atccatgaca cccgctttcc taaattgctt
     9481 ctaattatcc aagtaacaca ctgaagtgtc aacttatttg aataaaatta ttctttcttt
     9541 ggacgttctc tgatacgtct tgccacaaat gaataaagag caaggcaaaa aattgcaccc
     9601 aataatgctg gtatcaaata aaaaccgccg agttccggtc ccagtggtcc tagtaaaaat
     9661 tcgccgagcc aactacctaa aaaaccgata atgatatttc cagatacgcc tcccgggact
     9721 tcctttccta aaattgcgcc agctatcaaa cctaatatac cgccgataaa taacgaccat
     9781 aaaaaattca gcatctcctt tccctccttt atccatattt ctcactttta ttttacgctt
     9841 tattcagaag gactccaaaa atatgcgcca agaaactcca gaaaaaaaga agttgtaaca
     9901 aaagtgttca gcttatttcc gtagaagctg cttctggaac accgtgaatc agaggttgtg
     9961 aaatcagcgg tttgacctga gtattagaga aacaaacgga aaaatagttc ctcatatttt
    10021 gccgtttttt gagctaatat cgagggacag ctgattgaac atcgtttatt ggagaggttg
    10081 taaaaaagct gttctgcatc aagtaataag aacagccaag taaaaacagc tcctaatgtt
    10141 tttaacttgg ctggacttat taccgcagat gcaagctttt gaacaccgtt tattcgaaaa
    10201 aaaaggactg tgacacggct tttgtcacag tccccttttt tcgaataatt attgtccccc
    10261 tgtcaccttc acgatcacaa gtacattatt cctatgaatt aaattatttt tcttcatttt
    10321 tcgttgaacc atcttcgttc aacattgctt ttcttgacag atttacgcgt ccttgtttat
    10381 cgatttcagt tactttcaca aggatctcat cacctagttt aacaacatct tcgactttat
    10441 tgacacgatc gtttgctaat tgggaaatat ggacaagtcc atcttttcct ttgatcaagt
    10501 tgacgaatgc accgaatttt tcgatacgga caactttccc tagataaact tcaccaactt
    10561 tgacttcttt ggtcaaatct tcgatgatct tgatagcttt tttgatcatt tctgcatcag
    10621 ctgaagcaat acttactttt ccatcttgat cgatatcgat tttcacacca gtttcatcga
    10681 tgattccgtt gatcgtatcg ccgcctttac cgataacatc tttgatttta gcaggttcga
    10741 tctggatcat ttcgattttt ggtgcatatt ggcttaattc ttcacgtgga gcagcaatcg
    10801 ttgaagtcaa ttcttctaag atttccatac gtgcttgttt tgcttgagtt aatgcttcag
    10861 ttaggatttg ttctgtgatt ccttgaatct tgatatccat ctgtaaagca gtgataccat
    10921 cttttgtacc ggcaacttta aagtccatat cgccaagatg atcttccagc ccttggatat
    10981 cagttaggat cgtatagttt tcaccatcag aaacaagtcc catcgcaata ccggcgactg
    11041 gcgctttgat tggtacacct gcatccatca atgctaatgt gccagcacag atacttgctt
    11101 gggaagaaga accatttgat tccaatactt ctgctactaa acggatcgta taaggaaatt
    11161 cttcttctga agggattact tgtgctaacg cacgttcacc taatgcgcca tgtccgattt
    11221 cccgacgccc tggagagcct gcacgtcctg tagaaccaac agaaaattga gggaaattgt
    11281 agtggtggat gaatcgtttg ctgacttcca cccctaaacc atcaatgatt tggtgctctc
    11341 ctagaggtgc gagtgtacaa gctgataaag cttgtgtttg tcctcgtgtg aacaaacctg
    11401 atccatgtac acgtggcaat aagctgacct ctgatgacaa tggacggatt tcatccaatt
    11461 tacgcccgtc aggacggatc ttatcgatgg tgatcaattc acgtacgaca tctttttcaa
    11521 gatcttctgt gatttgttta acttctttca acaagtgatc ttcatcttca tgacctgcaa
    11581 atttctcagc ataagcttcg cggatatcga tcttcacctg ctcgatatta tcttcgcgag
    11641 caagtttttc ttcagtcatt actgctgctt tcattgtagc gtaagaagca tcaaagattt
    11701 cttttttcaa tacttcatca acttgcagta aagtcacttc cattttttct ttccctactg
    11761 cttgaacgat ttcttcttgg aaagcaacta actctttgat agcgtcaaaa ccaaacagca
    11821 atgcaccaag catgtcttct tctgaaactt ctttcgcgcc gctttcaacc atattgatgg
    11881 cttgtttcgt tccggcaaca gtcaattcga tatctgtttg ttctgcttgt tctactgttg
    11941 gattcaagac gtactcgcca tttacacgac caacttctac gcctgcgatt gggccgtcaa
    12001 atgggatatc tgagattgat agtgctaaag aagaacctag cattgctgcc atagctggcg
    12061 agcagtctgt ttctacactc atcacgatgt tcgttacttg gacttcatta cggaagcctt
    12121 ctgcaaacat cgggcgaatt ggacggtcga tcaaacgggc agtcaatgtt gcttccgtac
    12181 ttggacggcc ttctcgtttg atgaaacctc cagggatttt ccctgctgca tacatttttt
    12241 cttcataatt gatcgttaat gggaaaaagt ctgtatcttt tgcttcttta gaggccacag
    12301 ctgcacttaa gactactgta tctccataac ggactaaaac ggctccgttt gcttgttttg
    12361 ctagctgacc gacttcgatt tgtaagggac ggccgcccca tgtcgttttg aatacttgtt
    12421 tttctgacat aacttctcct tttccttctc gtgaaagcaa gacgctacta aacaaatgaa
    12481 aactgccgaa agccgtcttt tgagcagctt tcatttggtt agtaggagtc catctagctt
    12541 gatcacggaa ttttacttaa ttgatatacg agtaaaaaaa gtgagattca tgtgaatccc
    12601 actctttttg actaaaatta acgacgcaag cctaaacgtt tgatcaattc gcggtaacgt
    12661 tgaacgtcag ttttacgtaa gtaagctaac aagttacggc ggtgaccaac ttttttcatt
    12721 aggccgcggt atgaatgatg gtcttttttg tgcacgcgtg catgttcatt caagtggttg
    12781 atttcttcag ttaatacagc gatttgtact tctggagaac cagtatcccc ttcatggcga
    12841 gcgtattcgt tgatgatttc attcttgcgt tcttttgaaa ttgccatttc tttcacctct
    12901 ttcgattatt cccctgcgac tgagtaaaac gttggtgatt cgcttaacca agtggcaggt
    12961 catgtgccta gagcacacag ctatacttta cctgaaaccg ggttgaaaat caagtagtta
    13021 atcattaaga aaaggtagtt ttcaaacaat cttactacta cttttccatt tttgtggtaa
    13081 actataatgc gaaaatagac ttgaaacaca gtcttttaag aaaggggtac atattccatg
    13141 attacaatgg atgatatcat tcgtgaagga aatccgacct tacgcgaagt tgcaaaagaa
    13201 gtttctcttc cattaagcga agaagatatc tctttaggaa aagagatgct tgagttctta
    13261 aaaaacagcc aagatccaat caaagctgaa gaactacact tacgaggcgg tgttggctta
    13321 gctgctcctc aattagatat ttctaaaaga atcattgctg ttcacgtacc aagtcctgac
    13381 ccagaggcag atggaccttc tctaagtact gttatgtata atcctaaaat tttgagccat
    13441 tctgttcaag atgcttgttt aggggaaggt gaaggctgtt tatctgttga tcgagaagta
    13501 ccaggatatg ttgttcgcca tgccaaaatc actgtgtctt attatgatat gaatggtgaa
    13561 aaacacaaga ttcgtttgaa gaattatgaa tcgatcgtcg ttcaacatga aatcgaccat
    13621 atcaacggtg tgatgttcta tgaccacatc aacgatcaaa atccatttgc actaaaagaa
    13681 ggcgtattgg tgatcgagta gagaattggc aggttccttc gatactatgc tacaagaaag
    13741 ctttataaaa aaggggagtg ctcaaaagct gcacttccct tttttatgtt tctccactaa
    13801 tcgaaattta gatagacatc tagaaatcgc aattccaaaa aattctgtgt attggatacg
    13861 catcttttct tattttttct tatttcttcc atatgggtgt ttatctcaga gatgatagaa
    13921 tagtttctag gataaaaagt agcagctacg atgttatcct tcgtagagga ttcctgcttc
    13981 agctagtttt tgtttgatgt ttttcagcat atgcttttct gggtagcgga tcgtatgcag
    14041 atggatgccg cctgttaatt cggataacaa agtagcatta cttttttgat aacgtctcat
    14101 aaaggaatcg atatcttgtg gcgttttgat gtgcaaagtt cctgctaact caccgtaaag
    14161 gggatgttcc acaattacat caatgatttc accaccattt tcaacgatta gtctcagttc
    14221 ttcttctgtt tgatcttttc catgctggac agcgatttta ctgatgtgcc cagtattggt
    14281 ttcttctgtt tcgaggatat aacctcttgc tgttgccacg atacttttac ctgcagctcg
    14341 tagtaaagca atatcgccta caatgatctg acggctgact ccaaaagttt tcgcaaactt
    14401 gctagcactc actggttttt ctgtatttgc caaggtttgc atgatttgtt ttcgccgtgt
    14461 ctctccttcg atcacactta ttcctccttc attttgtttt ggtattcgcg cgagtgtttc
    14521 atcaaatcaa catacgtgtc tgttattgtt tccttatatc ccttattttt cagcagacta
    14581 acaactttat ccaatacttg ctgctctctt tttgcatcat aaactgcttg gttttcttct
    14641 tttttcactt gtcctatttt agttaccgta tccattcttt tttctaataa agcaaccaac
    14701 tctcgatcga tcatatcgat cttatgacgt aatgtttcaa gagactcttc tgttagttct
    14761 tcctctactg tcttgatgat acgagcagga tttcctgcaa tcactacatt gtcgggaaaa
    14821 gatttggtca caaccgaacc tgcgccgact actacgttgt cccctagagt gacacccggc
    14881 gtgacgataa ctcctccgcc taaccaaaca ttattaccga ttttaatggg ttttgcgtac
    14941 tccagaccgc tgttacgttt cactggatgt agtggatgcg tcgccgtgta aagctggaca
    15001 ttcggagcga acatgcagtt atcaccgatt tcaatcgtgc tgacatctaa aaatgtacaa
    15061 ttgaaatttg cataaaaatt ctccccaaca aatatattat atccgtaatc aaaattgata
    15121 actggctcca tatagatttt tttccctgta cggccaaaag tctctttcaa caattgggat
    15181 cgcagttccg ctgattctgc ttggttgatg atttggcttt gacttcttgc aaacatacgg
    15241 tcggcaacca gctcaggatc attggcaaaa taaagctcgc ctgcaatcat tttttctttt
    15301 tcggttttga attcagtcat aatctgctcc taattcttgt atttttttga aaacaaccgc
    15361 tccgtcttct tgaataaacg tataaggctc cgctaaataa acaggcgttt gattgattac
    15421 taagattttg gctgacggct ggcgaaactg taccagatca caaaacggat gaacttgaaa
    15481 actcgttcca acaatgacga tcagctctgc ttggctgacg gcataagccg cctgctccat
    15541 gactccatct tgaaaccctt caccatataa cacgatgtct gggcgtattt gtcctccgca
    15601 tgcttgatgt ttgtcgctgt gaagatattc tttccagtct accgtttgtt gacattttcg
    15661 acaatgacat tgatacagac tcccatgaaa attgacgagt tgcgggcttt tagcctttgc
    15721 gtgcaaaccg tcgatgtttt gggaaacaac ccaaacattt ttttctttcg ctagatgtgc
    15781 tatttccaaa tgaatgatat tgggttttgc ttcaggatga taaatctttt tgacaaattg
    15841 atagaattta tccggttcat tcaccattgc ttgatgactc agcaagtatt ccggctgctc
    15901 catcccgtgg tagattcctg acaaagatcg atagtctggc actccagaag gtgtggatac
    15961 ccctgcaccg gtcaaaaatg taatttgctg ataagctaat atctcttttg ctgcttcgtc
    16021 tactgttaat tgtttcatgt tttgtcacct cactttccat tatagcccat actcgaacaa
    16081 atttattgtt gaaaagtcag aaaaatggga ataaaaaagt gttatgggca tttgagcaat
    16141 ttatcacaat gttcactgac aaaagaatga aaacgtgtta caatactgct gtataaaagt
    16201 gaggtgaatc gatatgttac cattctttat ttattggggc gttattctgg cttgtatcgc
    16261 ctggttagcg ttaagcgttt atttctctgt tttttatctt gtacgcaaag aaaatggaaa
    16321 tctttgggct tttgctttat tcaacgtgat tgccgcaatt gttttagcca ttacattagc
    16381 cgtatatcga acatggggct ggggaatcac ccaatactca agtttgattt atctaatatt
    16441 agccatttat ggcgtcgttg ttcttttgca agcgatccta ggacgtgagc caaagaaagc
    16501 ggcagcttaa atgatcgaga tggaactgga cacaggaaat tttctgttct agttcttttt
    16561 ttattgtatt acatacattt ttgtgtataa ttatactatc gtttgcaatg gatagatgat
    16621 tcacattttg attgtttttt cggtaaaagt aaagatttct atatttatca gtattttttc
    16681 aaaaaccagc agttatagat tgacaaagcc tctcgagtat cgtaaacttt ttttgtataa
    16741 ataactgaat acgagatgcc tcaatcaatg aggtagaggt cgcgagaatc attaaccctg
    16801 tggagtgaag caaacatgga gaagcaggaa tagggaggat cgccgaaatg tgcacttttt
    16861 tgcttggagt gtatgttggg acgcaagtta agagcttgcg gactgtctta gcagtgatgc
    16921 taagttgcgc tatgttttgt gagaggttta tcaccttatg gaattttagc ggccttttta
    16981 tatctataaa tcggtcgctt tttatttttt cagataaagc tttaccgcat ttcatctaga
    17041 ccgctcgtgt atcatataat cagaaaggga gaaaaaaatg aaaaaaaata tggtttcagt
    17101 tgccgtacta tttgcttttg tcttctcact attcagtgtg ggatctctgg caaatgcaga
    17161 tgaaaaaaca ccgagcgatg agtttcgcgt aggtatggag gctgggtatg cacccttcaa
    17221 ctggtctcaa caaacagatg agaataatgc cttgccaatt caaggccaaa atagttacgc
    17281 cggcggttat gatgttcaaa tcgctaaaaa agtagcagat ggtttaggga aaaaattagt
    17341 catcgtccaa acgaaatggg atggtctggc tcctgccctt caatcaggca agatcgacgc
    17401 gatcatcgcc ggaatgagcc ctactgctga acgacgaaaa gaaattgatt ttaccgatcc
    17461 ttattacgaa tcacaattag tagtagtagt tcaaaaagat agcaaatatg caaaagcaaa
    17521 aagtttagca gatctttctg gagcaaagat cactgctcag ttaaatacat tccactatac
    17581 tgtgatcgat cagataccag atgtgcagaa acaacaagca atggataatt tctcagcaat
    17641 gagaacagct ttagcttctg gaatgatcga tggttatgtc agcgaacgtc cggaaggtgt
    17701 aacggcaact agtgtgaaca aagacttgga aatgcttgaa ttcagcggtg cagatggctt
    17761 ccagacaaac ccggaagatg ttcagatttc tgttggtatg cgtaaaggcg acccggatat
    17821 ccaaaaagta aaccagattc tttctggcat ctctaacgat gaacgaacca aaattatgga
    17881 ccaagcagtc aaagatcagc ctgctgcaac tgatagtgaa gaaaaacctg gcatcatttc
    17941 tgatttcact acgatttgga aacaatacgg caatatgttc ttacgaggtg ctggacttac
    18001 cttgttcatc gcattgatcg ggacagttgt cggtactaca cttggattac tgattggtgt
    18061 cttccgttcg gtacctgaat caggaaatgc actgactcgc ttcttccaaa aaatcggaaa
    18121 tgcacttctt tctatctatg tcgaagtatt ccgcggaaca ccgatgatgg tacaagcaat
    18181 ggtcatcttc tacggtctgg cactcgcttt tggcatttca ttgaaccgaa cgattgccgc
    18241 tttgtttatt gtctctctca atactggagc ttacatgaca gagatcgttc gtggggggat
    18301 ttttgcagta gatgaagggc agttcgaagc tgcgcaagcc attggtatga cccatggcca
    18361 aacgatgaga aaagttgtta ttccacaagt tttacggaat atcttgccag caacaggaaa
    18421 tgagtttgtt atcaatatca aagatacagc agttttaagt gtaatcggcg tagctgattt
    18481 gttcttccaa ggaaactctg cttccggtgc gaatttccaa ttcttccaaa catttacgat
    18541 cgtcgggatc atctacttag tcatgacctt tacgatcacc agaatcctgc gggcggtaga
    18601 aaagaaaatc gatggtcctt cagcttatac caagcttgaa gaaatcgaca attcaaattt
    18661 gcaggaataa ggaggaagaa tcatgaatga aaaaattatt gaaatcaaac atctaagaaa
    18721 aagcttcggt gaaaatgaag tattgaaaga tatcagcgta acagtcaata aaggtgaagt
    18781 tgtgacgatc atcggttctt ccggttctgg taaatctact ttcttgcgct gtatcaattt
    18841 actggaaaaa ccaacaggcg gcgagatcat ttataatgga gaaaatgtat tagctcctaa
    18901 atataattta cctaaatatc gaacgaatct aggtatggtt ttccaatcct tcaacctgtt
    18961 caacaatatg aatgtcttgg aaaactgtat gtctggtcaa acaactgtcc tgaaacgaga
    19021 taaagaaact gccaaaaaag tagcaatcga aaatctggaa aaagttggta tggatcgtta
    19081 tatcgaagcc aaaccagcac aattatctgg tggacagaaa cagcgtgtcg caattgctcg
    19141 ggcattatca atgaatccag atgtattgct attcgacgaa cctacttcgg cgttagaccc
    19201 tgaaatggtt ggcgaagtgc ttaatacgat caaagactta gcacatactg gcttgacaat
    19261 gcttatcgtg acgcatgaga tggaatttgc aaaagatgta tctgaccgtg tcatttttat
    19321 ggataaaggt gtgattgcag aagaaggtac accagaagat atctttgtcc atccgaaaga
    19381 agaacgtaca aaagaattcc tttcacgtat tttaaatgct taggatcaat agctgattgg
    19441 gacaaagagc tgcggtgttt accgcagttc ttttttatga attactcata aaaggtaaat
    19501 ttctgaaa
//
LOCUS       DACGLW010000054        19396 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.056, whole genome shotgun sequence.
ACCESSION   DACGLW010000054 DACGLW010000000
VERSION     DACGLW010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19396
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.056"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            70..2109
                     /locus_tag="IXT53_001902"
     CDS             70..2109
                     /locus_tag="IXT53_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HAQ4905707.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFQTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(2218..3369)
                     /locus_tag="IXT53_001903"
     CDS             complement(2218..3369)
                     /locus_tag="IXT53_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAQ4905708.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(3523..4053)
                     /locus_tag="IXT53_001904"
     CDS             complement(3523..4053)
                     /locus_tag="IXT53_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905709.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(4152..4502)
                     /locus_tag="IXT53_001905"
     CDS             complement(4152..4502)
                     /locus_tag="IXT53_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HAQ4905710.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(4659..6188)
                     /gene="ahpF"
                     /locus_tag="IXT53_001906"
     CDS             complement(4659..6188)
                     /gene="ahpF"
                     /locus_tag="IXT53_001906"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HAQ4905711.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(6198..6761)
                     /gene="ahpC"
                     /locus_tag="IXT53_001907"
     CDS             complement(6198..6761)
                     /gene="ahpC"
                     /locus_tag="IXT53_001907"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003756453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HAQ4905712.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(6905..7567)
                     /locus_tag="IXT53_001908"
     CDS             complement(6905..7567)
                     /locus_tag="IXT53_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ4905713.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            7751..9397
                     /locus_tag="IXT53_001909"
     CDS             7751..9397
                     /locus_tag="IXT53_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HAQ4905714.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            9388..10470
                     /gene="uxuA"
                     /locus_tag="IXT53_001910"
     CDS             9388..10470
                     /gene="uxuA"
                     /locus_tag="IXT53_001910"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HAQ4905715.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(10578..11480)
                     /locus_tag="IXT53_001911"
                     /pseudo
     CDS             complement(10578..11480)
                     /locus_tag="IXT53_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
     gene            11762..14806
                     /locus_tag="IXT53_001912"
     CDS             11762..14806
                     /locus_tag="IXT53_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005337696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SrtB-anchored collagen-binding adhesin"
                     /protein_id="HAQ4905716.1"
                     /translation="MKKILKRLATGFLAFATIVTALPTTAVHASETQYWTESAERAGY
                     IEKVMNDGSIGSTFNEGIMKVEGETAYCVDINTNFKNGYKTRADASTRMSADQISDVA
                     LSLEYVKQYAQSHSELNYKQVYLLEQCVVWQRLSVHLGWQCDNVRASYDEISKAVQDE
                     VYAGAKAFASENKGRYECGGYIYSGEGQDIGQFWAKLAVGNATLKKTSSNTSITDGNG
                     LYSIAGATYGIYSDKDCTKQLATLTTDNSGNTDTVEVKAGTVYIKELSAPAGYKVDST
                     VYSLNVEAGKTATLNVSDTPKVTDTLIELFKIDMETQKDAPQGDASLEGAEFTWKFYA
                     GHYTADNLPSEPTKTWVTKTIAEKDSDGTIHYVSRLSDEYKVSGDSFYTQDGKNVLPL
                     GTLTVEETKAPNGYLLDGAYMQANGSEEQIKGMYLTQITEDGDLAVLSGSNQYHVSDK
                     IIRGGVKIQKRDLETSDTKGQGSATLKDAEFEIISLNDNAVLVEGKLYSKNEVVKTIL
                     TDIEGVASTSADLLPYGKYRIEESKAPEGYLTDGAEPIEFEITEDGKIVDLTGTDTSI
                     YNQVKRGDLEGVKIGAGTHQRLAGVPFRITSKTTGESHIIVTDDNGQFSTSSDWASHK
                     NNTNAGKTSEDGIWFGTSEPDDSKGALIYDTYIIEELRCESNKGFELIPPFEIVVSRN
                     NVTVDLGTLTDEYEKEISIHTTATGKDGEKSIVAGKEVTIVDTVTLDGLEKGTKYQLK
                     GWQMLKEENAELLIDGQRVESDYTFTADSEEMKIEIEYTFNASSLGGQNLVTFEELYD
                     LSDEDEPVKVAEHKDIDDEGQTVLITEHIISIHTTATSEDGKKEIEAGKDVTIIDTVT
                     LDGLEIGTKYQLKGWQMIKDENAELIINGERVENDYTFTADSESMEVQITFTFDASEL
                     GGKELVTFEELYDLSNPDEPTKVAEHKDIEDDGQTVTITEVPETPEEPTEPEQPTTPD
                     TPTKTSDAPKTGDNTNIALFLGLLVLSGAGVAGTYFFKRRRS"
     gene            14995..15321
                     /locus_tag="IXT53_001913"
     CDS             14995..15321
                     /locus_tag="IXT53_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005337695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ4905717.1"
                     /translation="MKLKYIVPDMAQTFGNLEFAGENEVEQQRVNGRFVPVTRSYNLY
                     SDIQRADDIIVVLPAKAGEKRLKYEQKVKLVNPKITAEGKNVRGRGYTNYILTADDML
                     PAEESK"
     gene            15337..15729
                     /locus_tag="IXT53_001914"
     CDS             15337..15729
                     /locus_tag="IXT53_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003066616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ4905718.1"
                     /translation="MRLANGIVLDKEKTFGVLKFSALRHEVRVENEDGTTTEEIKRRT
                     YDLKCSQQERTIQVSIPAEIPVKDYPYNAEVELVNPVADTVANANYRGADVEWYIKAD
                     DIVLKNKGGQPTGNPQNHAPQGQQKNQK"
     gene            15733..15990
                     /locus_tag="IXT53_001915"
     CDS             15733..15990
                     /locus_tag="IXT53_001915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905719.1"
                     /translation="MENVAGYEDYEREFREVSLPEYRWLAQTMLTQAIEDSGCQMEGS
                     LIISMPDYISRDKCWYTFQFSIETIGWKNPASRIFTYIGCY"
     gene            16005..17384
                     /locus_tag="IXT53_001916"
     CDS             16005..17384
                     /locus_tag="IXT53_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ4905720.1"
                     /translation="MAFYKYKGKRIRASDKNLVYRFCAGTLLLLFVAILLLLNMGQLM
                     RTDWEHFSLLDNGVTLSTYNFITIGIATGVCALVAFLYYRFFHDSFKKLLHRQKLSRM
                     ILDNKWYEAQTVQDSGFFTDLQSRSREKIVWFPKIYYQMDTGLLHIRCEISLGKYQDQ
                     LLRLEDKLESGLYCELTDKTLHDGYIEYTLLYDMIANRITIDEVKAENGGLRLMKNLT
                     WEYDALPHALICGGTGGGKTYFLLTIIEALLQTNAQLYILDPKNADLADLGTVMDNVY
                     HTKEDMIECVNAFYEGMVQRSEEMKQHPDYKTGENYAYLGLPPCFLIFDEYVAFLEML
                     GTKESMSLLSQLKKIVMLGRQAGYFLIVACQRPDAKYFSDGIRDNFNFRVGLGRISEL
                     GYGMLFGSDVKKQFFQKRIKGRGYCDVGTSVISEFYTPLVPKGHDFLQTIGSLALARQ
                     AVSDERKEN"
     gene            17392..17796
                     /locus_tag="IXT53_001917"
     CDS             17392..17796
                     /locus_tag="IXT53_001917"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905721.1"
                     /translation="MGLLEMGYSDPTADLHVEGVCVDFDRFLEDLESVAGTTDDKCEE
                     FPTEAYHAHMEDILAEAGLGRLKLPLLFSVVLDEWLSIHGFNYRFTFLVVDKDFFRQI
                     YHEYEIDKDIARKCLSADTDVIVVYTGVTRVD"
     gene            18037..19227
                     /locus_tag="IXT53_001918"
     CDS             18037..19227
                     /locus_tag="IXT53_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ4905722.1"
                     /translation="MNESTFITALKEKRQSYGVSQTRLAIMAGISREHLNRIEAGKVT
                     LTDDMQDKLMEAVEKFNPDAPMFLLFDYVRIRFPTLDIQHVIKDILKLNVDYMLHEDY
                     GHYKYTEHYCLGDVFIYTSQDEEKGTLLELKGKGCRQFESYLLAQERSWYDFLMDALV
                     EGGVMKRLDLAINDRAGILDIPDLTAKCNREECVSLFRSFKSYASGELVKHNEQDKAD
                     MGHTLYIGSLKSEVYFCCYEKNYEQYAKLGVPIEEAPIKNRFEIRLKDERAYYAVREL
                     LTHYDAEQTAFSIINHYIRFVDKEPEKRKTDWKLNDRWAWFIGKDRPPIKLTTDPEPY
                     TLERTLGWISRQVAPTLKMLKKIDAGNSTSYLKEIEDNAKLTEKHLQIIRQQTADTEE
                     LVTE"
     gene            19243..19383
                     /locus_tag="IXT53_001919"
     CDS             19243..19383
                     /locus_tag="IXT53_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005337687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAQ4905723.1"
                     /translation="MAELVKDLVLVSLGMGIGVVLMCIAQVSKMADEQMEEIKKERID
                     KE"
BASE COUNT         6212 a         3856 c         4133 g         5195 t
ORIGIN      
        1 ttattatgtt agaataaaca ggtataaata gtgaaataaa ggaatgacaa gcaagagaag
       61 gaggaaaaaa tgaaaagaag tgacaagcac ggcaaaaatc gaacaggcgc ttatattgcc
      121 ggcgcagtga tcttaatagc agctgcgggt ggcggttatt tttactacca gcactatcaa
      181 gaaacccaag cagtagaagc tggagaaaag acagttgagc aatttgtcca agctttgaac
      241 aagggagatt ataacaaagc tgcagaaatg acatcaaaaa aggcggcaaa taaaagtgca
      301 ttatctgaaa aagagatctt agataaatac cagaatatat acggtgctgc tgatgtcaaa
      361 ggacttcaga tatcgaatct aaaagtagat aaaaaagacg attctactta cagtttttca
      421 tataaagcaa agatgaatac ctcattaggt gaattgaaag atctttctta taaaggaaca
      481 ttagacagaa atgatggtca aaccacgatc aactggcaac ccaacttggt ttttccagaa
      541 atggaaggaa atgacaaagt aagcctgacc acacaagaag cagcaagagg gaatattata
      601 gatcgaaatg gtgaaccatt agcaacaaca ggcaaactaa aacaattagg agtcgttcca
      661 agtaaacttg gagatggagg cgaaaaaaca gccaatatca aagcgattgc ttcctccttc
      721 gacttaacag aagatgctat caatcaggcg atttcacaaa gctgggtaca acccgattac
      781 tttgtcccat tgaaaatcat tgatggagca acgccagaac ttccagctgg agctaccatt
      841 caagaagtag acggcagata ttatcctttg ggtgaagcag ctgctcagct gattggttac
      901 gtgggagata tcacagcaga agatattgat aagaatccag aattaagcag taatggaaaa
      961 atcggtcgct caggtttgga gatggctttt gataaggatc ttcgtgggac aacaggtggg
     1021 aaattaagca ttacagatgc agacggtgta gagaaaaaag ttctgatcga acatgaagtt
     1081 caaaatggca aagatatcaa attgacaatc gatgcgaagg cacaaaaaac agcttttgac
     1141 agtctaggag gaaaagctgc atcaactgtt gcgacaacgc caaaaaccgg tgatcttctt
     1201 gcgcttgcta gctctccaag ctatgatcca aacaaaatga caaacgggat ctcacaagaa
     1261 gattacaaag cttatgaaga aaatcctgaa catccattca tcagccgatt tgcgacaggt
     1321 tatgctcctg gatctacgtt taaaatgatt acagcagcaa tcggtctcga caacggcact
     1381 atcgatccaa atgaagtgtt gacgatcaac gggcttaaat ggcaaaaaga cagttcttgg
     1441 ggatcttttc aaacaacgcg tgtcagtagt gatgtatcac aagtagactt aaaaactgct
     1501 ttgatctatt ccgataatat atatgcggca caagaaacgt tgaaaatggg tgagaaaaaa
     1561 tttcgtacag gcttagataa attcattttt ggtgaagacc ttgatttgcc aattagtatg
     1621 aatccagcac aaatttctaa tgaagatagc tttaactcag atatcttgct agctgatact
     1681 ggatatggac agggcgaact tctaattact cctatccagc aagcagcaat gtattctgtt
     1741 tttaccaaca atggcacact tgtctatcct aaattgattg cagataaaga gacaaaagat
     1801 aagaagaatg tcatcagcga aacagcagta caaacgatcg tgccagatct gagagaagtt
     1861 gtgcaagatg taaatggtac agcacattct ctttctgctt tagggattcc gttggcagcg
     1921 aaaactggta cagcggaaat caaagaaaaa caggatgtaa aggggcaaga gaacagtttc
     1981 ttgtttgctt tcaatcttga taaccaaggt tatatgatgg tcagcatgtt ggaaaataaa
     2041 gaagatgatg attcagcaac caaacgagca tcggaactat tgcaatacct caaccaaaat
     2101 tatcaataaa aaattgcttt ttaagtacag taaaaggact gtggcgttat cgtcacagtc
     2161 cttttttcaa ataaacggtt ttcacgatgt ctctttcaca acttcttttt gatttactca
     2221 cacttcataa actttttttg taaaatattt gagaacaagt ggagaaataa gtgtacttaa
     2281 taaaatcacg ataactaaag gtgaatagta ctgtggttcg atcaattgac tttgctgtcc
     2341 aatttgtaaa atgatcaaag ccatttctcc acgcgaaatc ataccagcac cgaccatcaa
     2401 tgaactatgt tgagaaaaac cggcaatctg agcaccaaaa taacctccaa gcaatttggt
     2461 caaaatcgct acaagcgtca atatagaaat aaagagtagt tgttcactaa agtcagaaaa
     2521 atcaacttct aatcctacac tgacaaaaaa tactggtata aatactgcat atcctaaagc
     2581 ctctacattg tggtatactt cttctttaac ctttgtctga ctgacagcta tcccagcaaa
     2641 aaaagcacca atgacagagc taagcccaac aagatccgct acgtaagaca ttcctaaaca
     2701 aataactagc gatacgatga taatagaaga atttgcgtac actttttctg caaatgaaag
     2761 taggaaaggc gcaatccatt tgactaataa gaaaataaaa acaaaataaa ttagctgttc
     2821 aatcaaaaca agcggtaaag aagcattgct agaagcttcc cccgttagaa aagataggct
     2881 gaaacttaga actaaaacga ctaaaatgtc atctaccaca gaagcaccca aaattgttga
     2941 tccttctttt gtattcacta cgtttagttc ttttaatact tctactgaaa tactgacaga
     3001 agttgccgca agaataatcc caaagaaaat cgcttcattt cccgaaactt ggaatgcttt
     3061 gcctgtcaac cagccaaaaa caaccgggaa gatgatccca agcaaagcaa cgaacatacc
     3121 aggtcggaaa tattttttca aaagagataa atcactttcc agaccagcta aaaacattaa
     3181 taagatcaca ccaatctccg aaaagtcatg gactagaata tcaggtttga cccaattcaa
     3241 tccagcttgg cctagcaaaa caccaacaag taattgtcca atcactgctg ggatacctaa
     3301 tctccgcgag atatgtgagc caatcgttgt tgaaatcaat atcaggcata aaataccaat
     3361 aaattccatt ctcatcattc ctttgtataa aatatagccc gaacttagca taacatacat
     3421 tattgaaaaa ataggaaaat atactcacaa aaaaaccaac aatccaaaag atttttttgg
     3481 attgttggtt tttatacggt ctattttaat aactaaaaat gattaacgta tagtcatttt
     3541 tcttattctg atgaaattga taatctcctg gatgcccatg aaaatcaaaa taaagccgaa
     3601 tatttggaat agaagcatca aacttttgaa tggattgaac aataagacga atccgccaat
     3661 caacaacaga atactactat aaacactcca acttttagct tttgtattca tgctgaagaa
     3721 caactggaac aagctgttga tgataacagc taatccaaga agaatcggca ggatagaaac
     3781 gattgcaggt gcaaaagcat agacgatcag agctaagatc aggaaaaaaa gaccactgaa
     3841 cagaccgata ctccaagaac ctgttttcct tttaaaacgg taagcctcaa aaagattcaa
     3901 caacccaagc aaaacaagat aggcggtgat cagataaccg ataaaatgaa aaacagcaga
     3961 agggtttagc acaatcgcaa tcccagaaat aatataaaca gcagctctta ataaggcgta
     4021 tttttgaata ttttgtttaa tcgtctcaaa catataagaa aacctccttt gacttatttt
     4081 aacgtgaact agtcatttta ccaaattaaa tgttacaaaa tgagcgaagg agaagaatat
     4141 ctctggaaaa atcaacttct gaggatcttt tccatttttt tgccttttgc tagctcatcg
     4201 atcattttat ctaaataacg aatttctttc atcaagttat cctcgatgtc ttctactcgt
     4261 acaccacaaa caacacctgt aatcaaggaa cgtgaaggat tggggacagg ggcttccgca
     4321 aaaaattgtt cgaaagtcac ttgtttttgt agttgatatt ctagttctgt ttgggaataa
     4381 cccgtcagcc aacaaatgat ttcatctact tcctctttta tacgatcttt tctctccgct
     4441 tttgctacat acataggata aacgcttgca aaacttgtcg taaaaatacg attttgcgcc
     4501 ataatcagtc acctttcttt ttttcaaatt atttactact tatagtatat aagaaaaaga
     4561 gaagaaaaag cgattattca aaaaaacaaa aaggattgtg acaaaattct tgtcaccatc
     4621 cttttttccg ctctctgact ttttacaatc ttcatagctc aagtgcgcat cagataatcg
     4681 aatgctccta aagctgcagt agctcctgaa cccattgaaa caatgatttg tttgtatgga
     4741 ctatctgtac aatcaccagc tgcgaaaacg ccggggatat tcgttgcgcc atttttctcg
     4801 gtcactattt catttcttgg attcatttcg atcgttccat ctagccaatc cgtattaggg
     4861 agtaagccga ctagaatgaa tactcctgaa acagctagtg tatgcatctc catcgtacgg
     4921 cgatcgatat aggagagaga ttctacctga ttcgtaccgc tgatttcttt cgtcgctgca
     4981 tgtttgataa cagtgacatt cgcaagagag actagtttat cctgtaagat ttgatctgct
     5041 tttaattctg gtaagaattc tagaacatat acatgttttg ctaacccagc caaatcgatt
     5101 gctgcttcaa caccagaatt accaccaccg ataacagcaa tctcttttcc tttaaataaa
     5161 gggccgtcac agtgtgggca ataagcaatt cctttatttt taaactcttt ttcgcctggt
     5221 acattgatag aacgccagtg cgcacctgtc gcgatgatgg cggtttttgc tttaagtgat
     5281 ataccgttag ctaagccaat attaataaaa tgttgctctt tatttaagga aacggcctgt
     5341 tgattcatca taatgtcgat agggtaacta cgcacatgac tttctacttg ctgcatcaat
     5401 tttggacctt ctgtgtaaga ggtaccaatc atattttcaa tccctagtgt ttcaacgact
     5461 tgtccgccga aagaatcagc gacaagtcct gtacggattc ctttacgtgc tgcatagata
     5521 gctgcactac tagctgctgg tccaccgcca atcactaata catcataagg ttctttttca
     5581 aacaactctt cttcaatcaa agggccagtg attttttcaa caatttgttc gatcgtcatg
     5641 cgtccgctac taaattcttc tccatttaaa aacacagttg gcactgccat gattttttta
     5701 tcatcgattt ctttttggaa catcccacct tcaatcatcg tatgagtaat atttggattt
     5761 aatacagcta atatattcaa tgcctgtaca acgtcggggc aattatgaca agtcaagcta
     5821 acataactgt caaaatgcaa tgtttgtgtt atttttttga ttttaagact caaagattca
     5881 ctgattttcg gtgcccgtcc actaatttgt aacatagcca gtaaaaaaga ggaaaattca
     5941 tgaccgaggg ggataccagc aaaaacgatt ccactttctt tgccaactgg attgatagaa
     6001 aaactaggtg tgcgcatcga atctcttttt tcccaagaga ttttaggaga catttctgct
     6061 atttccttcg taaattcagc tagtttttga ctattttcac tattatccaa actaacagaa
     6121 aaaacaatag gagattcaat cagtgctaaa tattgttcga gattttgttt agtttctttg
     6181 tctaacatat ccaccaccta aatctttcca acaagatcaa aacttggttt caatgtttcg
     6241 ccactttctt tccattttgc cggacaaact tcacctgggt gggtacgtac atattgagca
     6301 gcgcgaatct tatcaatcag tgcactagcg tcacgaccga ttccatctgc attgatttcc
     6361 atagcttgaa cgattccgtc tggatcaata ataaatgttc ctcgctgagc taatccttgc
     6421 tcttcatcca aaacatcaaa aatacgagaa atctgatgag acggatcacc gatcatcacg
     6481 tattcgattt taccgatagc atctgatgta tcatgccatg ctttgtgagt aaagtgggta
     6541 tcggtagagc aagaaaagac ttcaacacct aaagatttca atgtactgta ttgttcttgt
     6601 aagtcttcta attcagtagg acaaacaaaa gtaaaatcag ctggatagaa gcaaagaata
     6661 ctccagttcc ccataagatc tttttcactg acttctaaaa attctccttg atgataagca
     6721 tgtgcattaa attcttcgat ttttttccca atcaatgaca tagtaaaacc tccaataata
     6781 attatttatt tataattatt gtaatttagt tggagttgca aagtcaatgg atattttgtt
     6841 atttaataaa gattactcaa aaacgaaggt taattataaa aatagaagag gtcgcaagaa
     6901 aatgctactg ctggaaatac tcgggataac gttgagtaac agtttcctgt tctttcagca
     6961 tcatgtgtaa gtgcaatccc gtttgaaatc gagcttcttc tggtttttta tcaactaaag
     7021 ccttgagtaa atcataatgt tgttccatga tcgtttccca tggaagcccc tgaatggtca
     7081 ctcgtaacca gcggaaacgt tctaaatggg tattatggtc atctaaccag ttccaaactt
     7141 cttttcttcc tgcaatttcg aaacaagctt catgaaataa attatctgca cggaaaaaag
     7201 cgcgttcgtc atttattttg actgcttttt cttgttcttc taaaatcgtt tctagcacta
     7261 ttcgtgtttg tgtgtccaat ttggaacaac attcaatcat gatttgctct tccaactgtt
     7321 ctcggacaaa gcgagcattt tctgcagaaa caagattaat tttggaaaca tacgtaccac
     7381 tttgaggtat cgtatacacg agttcttgct gacgtagttg aatcaatgct tcgcgaatgg
     7441 gagtccggcc gatagcaaga tgttcctcca gtgccttttc agatattttc cttccaggtt
     7501 ctaagtcagc atagataatt tgtttacgaa tcgcttgata agcttgtgat tgcaagctgc
     7561 ttttcatctt tattcctcca aaatcatttc tatgaacgta gtataacaga agaggtctga
     7621 aaaacaatct ggtatgtcac attaactagt aaatttgtat aaatagtaaa attttggttg
     7681 acatagcgat ttttattgca taaaattcac ttgaatacaa aaactaatat gttagtttag
     7741 gaggggaaaa atgattgatt taaacaactg gaggaacttt atccctgaat tatctcaaaa
     7801 agaagtagaa atacctcatt ttgaccgtgc atcattaaga gaaaatggac aaaaaaatcc
     7861 tgtctggatc catttcggtg gcggaaatct ttatcgagga tttcatgcag aaatcgctca
     7921 acgtttagcg gatctaggcg aattgtccac tggtgtcatt gtttgtgaaa cattcgatga
     7981 agaaataatt gaaagggctt accaagattt tgatgatttg attcttgaag tggtcatgcc
     8041 aagcagtgga gagttcgtca aacgtatatt agctgcgaca gctgacagtc tgtattgtca
     8101 tccagattgt tctgaacact ttgataagat gattcgacac tttgaaaatc ctagtttaca
     8161 attagttact tttgctatta ctgaaaaagg ttatcgattg aaagatacaa ataataaata
     8221 tataggaccg attgcagtcg atataaaaaa tggtccaaag aagccatcac atacaatgag
     8281 tattcttgct gtgctacttt ataaacgttt tttatctgga gcttggccga ttgcacttgt
     8341 ctctacagat aatttttcac aaaacggtag tcaattacgt acttctttgc taacgatcgc
     8401 aaaagaatgg caaaaggaag gttatgtaca aaatacattt atccaatact tatcgaatga
     8461 agaaattgtt gcgtttccat ggacgatgat tgatcgtatc accccgaatc cttccttgtc
     8521 agttagcaaa atgttggcaa aagagggctt ttcaaatatg tcgattttcc aaaccgcaaa
     8581 aggaaataat attgctccat ttgtcaatac agaggacatc cattatttaa tcatggaaga
     8641 tcattttccc aatggacgtc cgaaattcga aaaagctggg gtattcttga ctgatcgtgc
     8701 aacggtagat aaagcagata cgatgaaggt gacgacttgc cttaatcctt tgcatacgtc
     8761 tttagctatt atgggttgtt tactggggta taggagcata gctgaggaga tgaaagatcc
     8821 agatttatcg ctgctagttt ctgaaatcgg gtacaatgaa ggcctcccag ttgtggagtc
     8881 tccaggtatc attgatccaa agatctttct ggaagaagta attactgtgc gcttttctaa
     8941 tccaaatatt cctaatatgc ctcagcgaat cgccacggat acctctcaaa aggtggcgat
     9001 tcgttatggt gagacgatta aaaaatatat ggaaatagaa gttaaagacc cgcagacact
     9061 tgtattcata ccattgactc tcgctgcttg gcttcgatac cttattggca ttgatgatca
     9121 aggaaaacca ttcatcccga gtccagaccc actattgaca gaccttcagc ataagctttc
     9181 cgatattcaa ctgggtacgc aagatacatc acatattcat gagaaaatgg ctcctatttt
     9241 gtcaaataaa gaaatttttg gtgtagatct ttaccaagca gagcttggca gaaaaataga
     9301 agaaatgttc atcaagatgg tagcaggacc aaatgccgta cgaaaaacat tacaggatta
     9361 cttacaagaa catggaggaa atttatcatg gaaatgactt ttagatggta cggatcacag
     9421 gaagaaaaga ttaccttaga tgaaatcaga cagattcctg cggtcaaagg gattgtaggc
     9481 acattgatgg atataccagt aggagaggta tggcctaggg agaggatcaa agaactaaaa
     9541 gaagaaattg agtctcgtgg gttaacacta aaagtaattg aaagcgtcaa tgtgcatgat
     9601 gccattaaaa ttggattacc ttcccgtgag cagtatatcg aaaactataa gcagacaatc
     9661 cgaaacttgg cagaatatgg aatagaagtc atctgctata attttatgcc tgtatttgac
     9721 tgggtgaaat ctgaccttga ctaccgctta gaagacggct catccacgtt agcttttata
     9781 tctgatgata ttccagatga tccaagagaa atcgtcgcgc gcatcgaaca ttccagcaat
     9841 gaatttggat tgccgggatg ggaacctgag cgcttagctc ctatcaaatc cttgcttgaa
     9901 gcctatgcat cagtagacga agaaaaactt cgagaaaatt ttgcctattt tctccaatca
     9961 atcatcccaa catgtgaaga ggtaggggtc aaaatggctg tacatccaga tgatccgcca
    10021 tactcgattt ttggattagc tcgaatcgta aagaatcgag aagatttaga ttggttgtgt
    10081 cgcatagtag acagccctta taacggaatc actctttgta cagggagtat tgcagaagat
    10141 ccagaaaatg atgtatatgg gattttagca gaattttgcc aaagagatcg aatcccattt
    10201 gcacatgttc gaaatattaa atttttagct aatcgagatt tctatgaggc accacataaa
    10261 tccgaatacg gttcattaga catgtaccgc atactaaaaa caatgtatga caacggattt
    10321 aacggataca tccgtcctga tcatggaaga atgatctgga atgagacagg aagaccagga
    10381 tacggcttat atgatcgagc attaggtgct gtttatctaa atggattatg ggaagcaatc
    10441 gaaaaaggaa acaaaagtaa agataattag tgaaaaaaac tgtaatttta ttatgtaaat
    10501 atgaaggatg ttcaaattct tgatacataa atccggaggt tgttaataag cattcggatt
    10561 tatgtatctt atttggtcta ggaagtaact tgattttctt ttttcgattg atccatgagt
    10621 gcttcttctt ttaattcaaa gatatattct gagatcctca taatcacttt tttccagtct
    10681 tctggtgtga tgattccttt taaaatatag atttttttga tatgagaagg caattgtttc
    10741 aaagggcagt cgctgataac gatgttgcag ttctccataa aatcattttg tattttgatc
    10801 gattcatttg aaaaaatttg ttctatctta acgatcagat ctttactgat ataaaaatca
    10861 cgcgtatagt tcacaccaat cacaattcgc ggctgttttt tgtagcgatc atacaaatgt
    10921 actaggtctt caaccatcca ttgcacattt tccttttcta aaataactgg taacaagtgt
    10981 tgattttgtt ctctgaaatc catataaaat tgttcaatgg cggtttggac ttctttcaat
    11041 acgctatgtt cttgataaag catgaaagta ggaaggggcg tatttggttg attgaagtta
    11101 agaaatccat tataaagttt atttcgtgcg aatgccaatg agtaaagaat cttttctttt
    11161 tcaggtataa acaaatcaaa tacagtacaa agggactcca ccatttctct tgaataaaga
    11221 acatgaggaa cgtttgcttt ttttaaaagt ttgtattgct ccaagcttgt ttcttccgat
    11281 tcgattttcg tgatagaaag tcgagctaaa atattcacta agatgcgttg ttcgcgacaa
    11341 attggcagat taggtcgcaa aatatctaca gagagatcaa taaaaaagtc aatatattca
    11401 tactctgtga attctttttg tacgtctttc acagaagttg caggaaaatt ctctttacat
    11461 atttttaaag gaaaaaacaa ctaaaaatct gattgtccgc taaaacactg tacttatcag
    11521 cacattcaag gattgaaata cttcaaatta ctacgatttt actaccattg aatgagcctt
    11581 gatacgttag aaatcccaga tatgcaaaga tatggtacag cacatcagta ttgaataaat
    11641 gaaaaattga atatcgcaag tagattatgg aacgccgata ttgacgttcc ttttctattt
    11701 ccagccgttc agaaatggac ggctttttct atatccaaac gaaaggagaa ttttaaaagc
    11761 tatgaaaaag atattgaagc gattagccac aggcttcctt gcctttgcga ctatcgttac
    11821 cgctttgccg acaacggcag tccacgcttc tgaaacgcaa tactggacag aaagtgcgga
    11881 gcgagcagga tatatcgaaa aggttatgaa tgacggttct atcggttcga cctttaatga
    11941 gggcattatg aaagttgagg gcgaaactgc ctactgcgtg gacatcaata ccaacttcaa
    12001 gaacggctac aagaccagag ccgacgcaag cacacgcatg agtgccgacc agatttccga
    12061 cgttgccctt tctctggaat acgtcaagca gtacgcacag agccacagcg agctgaatta
    12121 taaacaggtc tatcttcttg aacagtgcgt cgtatggcag agattgagcg ttcatcttgg
    12181 ctggcaatgc gataacgtca gagcttccta tgatgaaatc tcaaaagccg tacaggacga
    12241 ggtgtacgct ggggcgaaag cctttgccag cgagaacaag ggacgctatg aatgtggcgg
    12301 ttatatctac tctggcgagg gacaggacat tggacagttc tgggcgaaac ttgccgtagg
    12361 gaacgctact cttaaaaaga cttccagtaa taccagcatt accgacggga acgggcttta
    12421 ctctatcgct ggtgcgactt atggtatcta ttctgataag gactgtacga aacagcttgc
    12481 cacccttacg actgataaca gcggaaatac tgataccgtg gaagtaaagg ctggcacagt
    12541 ctacatcaaa gagctttctg ctcctgctgg ctataaggta gacagcactg tgtattcctt
    12601 aaatgtggaa gctggcaaga cagcgacact caatgtatca gatacgccaa aggttacaga
    12661 cacattgatt gaattattca agattgacat ggaaacacag aaagacgctc cacagggcga
    12721 tgcatcccta gagggtgcgg agttcacatg gaaattctat gcgggacact ataccgctga
    12781 caatctccct agtgagccta caaaaacatg ggtgacaaag acaatcgctg aaaaagacag
    12841 cgacggcact atccattacg tttcccgcct ttctgatgaa tacaaggtat ctggggacag
    12901 cttctataca caggacggaa agaacgtgct tccattagga acactgaccg ttgaggaaac
    12961 gaaagctcct aatggttatc ttcttgacgg tgcatatatg caggcaaatg gtagcgaaga
    13021 acagattaag ggaatgtacc ttacacagat taccgaggac ggcgaccttg ccgtactgtc
    13081 tggaagcaat cagtatcatg tatcagataa aatcattaga ggtggtgtaa aaatccaaaa
    13141 aagagattta gaaaccagcg ataccaaagg tcaaggaagt gctactttga aagacgctga
    13201 atttgaaatc atctccctta atgacaatgc cgtacttgtc gaggggaaac tttacagcaa
    13261 gaatgaagtg gtaaagacaa tccttaccga cattgagggc gtggcttcta cttctgctga
    13321 cctgttacct tacgggaaat accgtatcga agaaagcaaa gctccagagg gctatctgac
    13381 agacggtgca gaaccgattg aatttgaaat cacagaggac ggaaaaatcg ttgaccttac
    13441 aggtacggac acatcaatct acaatcaagt gaagcgtggc gacctagagg gtgtcaagat
    13501 tggggctggc acacaccagc gtcttgcggg agttccgttt aggatcacaa gcaagaccac
    13561 aggtgagagc cacattatcg taactgatga taacgggcag ttctccactt cttccgactg
    13621 ggcttcccat aagaacaaca ccaacgcagg aaagaccagc gaggacggta tctggtttgg
    13681 aacaagcgag ccagacgaca gcaaaggtgc tttgatttac gatacctata tcattgagga
    13741 attacgttgt gaaagcaaca agggctttga gcttatccca ccgtttgaaa tcgtggtgtc
    13801 cagaaataat gtgaccgttg acttagggac actgactgat gaatacgaga aagaaatctc
    13861 tatccatacc acagccacag gcaaggacgg cgagaagtct atcgtggctg gcaaagaggt
    13921 aacaatcgtt gatacggtta cacttgacgg actggaaaaa ggtacgaagt atcagctcaa
    13981 aggctggcag atgttgaaag aggaaaatgc cgagcttctt atcgacggac agcgtgtgga
    14041 aagtgattac acattcacag ccgacagcga agaaatgaag attgaaatag aatacacgtt
    14101 taacgcttct tctctgggcg gtcagaatct tgtgacattt gaggaacttt atgacctttc
    14161 cgacgaggac gagcctgtaa aagtagctga acacaaggac attgacgacg agggacagac
    14221 ggtgcttatc acagagcata tcatctctat ccataccaca gcgaccagcg aggacggaaa
    14281 gaaagagatt gaagctggaa aagatgtgac aatcattgat accgttaccc ttgacggctt
    14341 ggaaatcggc acaaagtatc agcttaaagg ctggcagatg attaaggacg aaaacgctga
    14401 gcttatcatc aatggtgaac gtgttgaaaa tgactatacc tttactgccg acagtgaaag
    14461 catggaagtc cagattacct ttacattcga cgcttccgag cttggcggga aagagcttgt
    14521 gacctttgag gaattatacg atttgagcaa tccagacgaa ccgactaagg ttgcagagca
    14581 taaggacatt gaggacgacg gacagactgt gacaatcaca gaagtaccag aaacgccgga
    14641 agaaccaaca gagccggaac agccgaccac accagataca cctaccaaaa caagcgacgc
    14701 tccaaagaca ggcgataaca caaacattgc cttattctta gggcttcttg ttctctctgg
    14761 tgcaggcgtg gcaggaacat acttcttcaa gcgtagacgt tcataatcgg acggtctgct
    14821 tatgatgaag aaatcacgtt ccccgccgta tgtacttata acaggaaatg aaaatttaaa
    14881 ctgttccttt tctgttaatc tgcttgcgga ttgacggaaa agaacagtcc actaggggcg
    14941 tggggagtgt agctccccac atatcaatca cagaaagaaa tgaggtatga acacatgaaa
    15001 ttgaaatata tcgtgcctga catggcacag acttttggta acttggagtt tgcaggagaa
    15061 aatgaagtgg agcaacagcg tgtcaatggt cgctttgttc ccgtgacgag aagctacaac
    15121 ctgtattccg atattcagag agccgacgac attatcgtgg tgcttcccgc aaaggctggg
    15181 gaaaagcgtt tgaagtatga gcagaaagta aagctcgtca atcctaagat taccgccgag
    15241 ggaaagaacg tgcgaggtcg tggctacacc aactatatct taacggctga tgatatgctt
    15301 ccagccgagg aaagcaagta aagggggtat cagattatga gattagcgaa tggaattgtg
    15361 ttagataagg aaaagacgtt tggagtgctg aaattctcgg cattaagaca tgaagtgcgt
    15421 gtggagaatg aggacgggac gacaaccgag gaaatcaaac gtagaactta cgatttgaag
    15481 tgcagtcagc aggaacgcac gatacaggta tctatcccag cggaaatccc tgtcaaggac
    15541 tatccgtaca atgccgaggt ggagcttgtc aatcccgttg ccgataccgt ggcaaatgcc
    15601 aactaccgag gggcagatgt ggagtggtac atcaaggcag acgacattgt gctgaaaaat
    15661 aaaggcggtc agcccacagg caatccacag aaccatgctc cacagggaca gcagaaaaat
    15721 cagaagtagc ctatggagaa cgtagcaggc tatgaagatt atgaaaggga gttccgagaa
    15781 gtctcgcttc cagaataccg ctggctggct caaactatgc tgacgcaggc aatcgaggat
    15841 agtggctgtc aaatggaagg tagtttgatt atctccatgc ccgattatat cagcagggac
    15901 aaatgctggt atacattcca gttttcgatt gagacgatag gctggaaaaa tccagccagc
    15961 aggatattta cctatatcgg ctgttactga tgaaaggagt atcaatggca ttttacaaat
    16021 acaagggaaa gcgtatcaga gccagcgaca agaaccttgt gtaccgcttc tgtgcaggta
    16081 cgctcctgct cctgttcgtg gctattctcc tgttactgaa catgggacaa ctcatgcgta
    16141 ccgactggga gcatttcagt ttactagata atggcgtgac gctttctaca tacaacttca
    16201 taaccatagg gatagcgact ggtgtttgtg cattggtcgc ttttctttat taccgcttct
    16261 tccatgacag cttcaaaaag ctattacacc gtcaaaagct ctcccgcatg atactggaca
    16321 ataagtggta tgaagcccag accgtacagg acagcggttt tttcactgac ctgcagagca
    16381 gatcaaggga aaaaatcgtc tggtttccta aaatctatta tcaaatggac acgggactgc
    16441 tccatatccg ctgtgaaatc tctttgggaa agtatcaaga ccagctcctt agactggaag
    16501 ataagctgga aagcgggctt tactgcgagc tgaccgacaa gacgctccat gacggctata
    16561 tcgagtacac cctgctctat gacatgatag cgaaccgtat cacgattgac gaggtaaagg
    16621 cggaaaacgg gggcttgcgt ctgatgaaga acctcacatg ggaatatgac gcactccctc
    16681 acgcccttat ctgtggcggg actggcggtg gaaagaccta cttcctgctg acaatcattg
    16741 aagccctctt gcagaccaac gcccagcttt acatcttaga ccctaagaac gctgaccttg
    16801 cggacttggg tacggtcatg gataacgtgt accacaccaa agaagatatg atagagtgcg
    16861 tcaatgcgtt ctatgagggc atggtacagc gaagcgagga aatgaaacag cacccagact
    16921 acaagacggg cgagaactat gcctatttgg gcttaccgcc ctgcttcctt atctttgacg
    16981 agtatgtggc gttcttggaa atgctgggga caaaggaaag catgagcctt ttaagccagc
    17041 taaagaaaat cgtcatgctt ggcagacagg cgggctattt cctgattgtc gcctgccagc
    17101 gtcccgacgc aaagtatttc agcgacggta tcagagataa cttcaacttc cgtgtgggct
    17161 tgggacgtat cagcgaactt ggctacggta tgctctttgg aagcgacgta aagaagcagt
    17221 tcttccagaa gcgtatcaag ggacgtggct attgtgatgt gggtacgagc gtcatatcgg
    17281 agttttacac tccccttgta ccaaaggggc atgatttttt acagactatc ggctctcttg
    17341 cactggcaag gcaagccgta tcagatgaac gaaaggagaa ttgaaaaata tatgggactt
    17401 ttagaaatgg gttacagcga ccctaccgca gatttgcacg tcgagggtgt ctgcgtagac
    17461 tttgaccgct tccttgagga tttggaaagt gtcgctggca cgactgacga taaatgtgag
    17521 gaatttccga cagaagccta tcatgcacac atggaagata ttctggcaga agcgggactt
    17581 gggagattga aacttccgtt gctcttttcc gtggtgctgg acgaatggct ttccattcat
    17641 ggcttcaact accgttttac gtttcttgtg gtggataagg attttttcag acagatatac
    17701 catgaatatg agattgacaa ggatatagcc agaaaatgcc tttctgctga taccgacgtt
    17761 atcgtagttt atacgggcgt aacaagggtt gactgaacca ctgacggctg gaacggtcag
    17821 cgaagcgacc cagaccgtgt aagccgtcct gtggagcagg gcttgccctg ctccacgaaa
    17881 gaagcccttc ggtatctaac agaggggcac aattccgctg gaaactacgg tcaaaaagtc
    17941 tggaaatccc gcttggtata aggattttct gaaactactg actgactgtg acaatcggcg
    18001 gaaaatatca cactttcacg attgggggta tgcaggctga atgaaagcac ctttataacc
    18061 gccttgaaag agaaacgcca gtcttacggc gtgtcccaga caaggcttgc aatcatggct
    18121 ggtatcagcc gtgaacactt gaaccgtatc gaagcgggca aggtaacgct cacggacgat
    18181 atgcaggata agctcatgga agcggttgag aaattcaatc ccgacgctcc tatgttcctg
    18241 ctgttcgact atgtgcgtat ccgtttcccc acgctggata tacagcacgt tatcaaagat
    18301 atactaaagc tcaatgttga ctatatgctc catgaggact acggacacta caaatacacc
    18361 gagcattact gtctgggtga tgtgttcatc tatacctcgc aggacgaaga aaaaggcacg
    18421 cttctggagc tgaaaggaaa aggctgtcgc cagtttgaaa gctatctgct ggcacaggag
    18481 cgaagctggt acgattttct catggacgct ctggttgagg gcggtgtgat gaaacgcctt
    18541 gaccttgcca ttaacgacag ggcgggcata ctggatattc cagatctgac cgccaaatgc
    18601 aaccgtgagg aatgtgtttc cctgttccgt tctttcaagt cctatgcttc tggcgagctg
    18661 gtgaagcaca acgagcagga caaggcggac atgggacaca cgctgtatat cggttctctg
    18721 aaatcggaag tctacttctg ctgttatgag aagaactacg aacagtacgc aaagctgggc
    18781 gtgccgattg aggaagcacc gattaagaac cgcttcgaga tacgattgaa agatgaaaga
    18841 gcttattatg ccgtccgtga actgctgacc cattatgacg cagaacagac ggcattttct
    18901 atcatcaatc actatatccg ctttgtggac aaagagccgg agaagcgaaa gacagactgg
    18961 aagctcaatg accgctgggc gtggtttatc ggaaaagacc gaccacctat caagctgaca
    19021 acagacccag agccgtatac tctggaaagg acgctgggct ggataagcag gcaggtcgcc
    19081 cctacgctga aaatgctgaa aaagatagac gctggcaaca gcacaagcta tctgaaagag
    19141 attgaggaca acgcaaagct gacggaaaag catttgcaga taatcagaca gcagacagcg
    19201 gacacagagg aacttgtcac agagtaaagg agaattttga ctatggcaga acttgtcaag
    19261 gacttggtgc tggtatcgct gggaatgggt atcggtgtcg tccttatgtg tatcgcacag
    19321 gtcagcaaaa tggctgacga gcaaatggaa gaaatcaaaa aagaaaggat tgataaagaa
    19381 tgaatttcgg acagaa
//
LOCUS       DACGLW010000055        18968 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.057, whole genome shotgun sequence.
ACCESSION   DACGLW010000055 DACGLW010000000
VERSION     DACGLW010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18968
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.057"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            complement(341..2311)
                     /locus_tag="IXT53_001920"
     CDS             complement(341..2311)
                     /locus_tag="IXT53_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905724.1"
                     /translation="MEIMGRSQLLNFKKKRKTKKEQKTRNSKVVNNTFVSDLTSLVGY
                     QFTPSYIRTGKRWGTIVKLVNTYGMNRNYGFGWMVKAIPEVRVEGVKAYLIEADKPVS
                     QKDQQEIMHENVGGSTNSYENNLEGKNSDAEISKTVMRIMDLNTASLLDSMANKIIDW
                     KLEILLVSDSPNKISEQLIKLQRLYDEKMYGLKLMSYGGDQRDLFDRLLSEPIGNVYE
                     YTSMSSDFAGNDHAIRKGLDDANGWAIGTMSTSYASGVAFMALDDSFTSKVLVASHKN
                     ATIKGYDEKISASSLWGQLIANNAMANHKRTFHIVLNKFDYFGDPTGRTFVAKPSLNS
                     VIEKVNLAEEGLNSLELFGNQKDIGDLYRRNLDKLTQIFYLLAGRTLDSATRVLLKKE
                     LGNFYISQKLWDINYEERSKLLVNSSDTMPVLGDFISYLTNAETKAKKSGTEKEVDRI
                     IVLKDTLRTALDSFSRMFNQRTTLKEIGKSDKLQFYYNLGDLRAETNVMEAQFLNTFD
                     FITSQAKKDDIVMIHGFDKLSIETVGLIKKRLTMLEEDGIRLAFLFDTIGSGEDNEYK
                     EDIETADIFNTDGKIYQSFESDFNYTIIGKMTSGELMKYEKKVAPARKGEDDQKLSVD
                     LRQYLTTGRSNKFQIRRPVDLTNNFVIGEFIL"
     gene            complement(2326..3192)
                     /locus_tag="IXT53_001921"
     CDS             complement(2326..3192)
                     /locus_tag="IXT53_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905725.1"
                     /translation="MADNSNYGFKHNLHQKPSETIVRVEWLEKHVNSTYSVTAKRLIV
                     SAIVVAVVVLGSIVYLVSDTIKLKFIFVPTLLMIIGFLILISQYMFIDPSSGSLISGS
                     IRLFKSVVNSARVSMGKKKPFRGTEILKVKQDGTIVYRDGSVALMFNLDGATSLTAFP
                     EEVMAQESKSIQYQNTRDRSLAEIKITSSQPQNTERQTRNMKIQKRNARSNKAVFDII
                     DQQERFVSEYIDGVKTTVIQYLILKSQSKMVLDEGVERLENYTSRGLYYSVTQLSAKQ
                     TKEILSDIYALK"
     gene            complement(3182..3415)
                     /locus_tag="IXT53_001922"
     CDS             complement(3182..3415)
                     /locus_tag="IXT53_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HAQ4905726.1"
                     /translation="MREEKIVYPVIQNNQENMTSSQHLQEAQLVYQVNELPQTNETMS
                     KSISLIGLLIVAIVITVGVYRKLNVKGSISDGR"
     gene            complement(3427..5583)
                     /locus_tag="IXT53_001923"
     CDS             complement(3427..5583)
                     /locus_tag="IXT53_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905727.1"
                     /translation="MAKFKDITESINYVAETVPVVALSLKPQLLEVYKKEEKEAKAKK
                     AKAKVWKEFDALLTKDLPKKNYMVIEDVQKLNSEGVIDEDDAILDSEKYVVLLKSSLN
                     YVADLPEKFKEKLLAGVRFMDSSGQLLVIPDDPNVFESEVEEATDFYSAVEQLEVATN
                     GLERIAETFNSSEEVEFEAVAENVPDEKEALFVDDNKPTEAENQEVSIEETQIQTETY
                     QQPKIDEKPESQSDKENYVYEAEKITSESDVPKNLDADFLEKNFEQIIADKFQPVVLD
                     EINDPVDLNEEMLSIPELKPLLFLAKESMNARISIANNKINSELNKTKEQLILKGKQM
                     LADELLRIEEATSLNGQNQFTNTLSVAKESYEAEASKIDDLVMEKSNIIRKDYDMAKQ
                     AYLDAELKKLEQRYDAEHLPEVKQQEDEYRKATEESLDQQYQEAIESIKNDALKQSQE
                     AKEVVVERIINSLKPEIEKSLTDVQEKTLEIVEKTKSENEAEFHDTKEKTTDVVVKYF
                     ANKEEVEKQVANNTKELETKLVEQQKTIEEYKDRMKEVKDLAEGYKLNSEKLAEQSEA
                     LREDLSRSRSDLAQMAEKTKSLAVATENQSSSKRDFTSKKMATIISVAAMACGTILGS
                     SYFISHNNQQAQAQTQTSISRVENSIGTNTNSASTNSSYEATPTTEFKVGKDVVISID
                     GKTTKAEVISIDGEKAVVKTPDGNQYKIQAHLSDNN"
     gene            complement(5603..5824)
                     /locus_tag="IXT53_001924"
     CDS             complement(5603..5824)
                     /locus_tag="IXT53_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905728.1"
                     /translation="MVFDREKMLAHANEVLMSSLKGTELAKIMNMNVNQFYDYRNGSK
                     KIEKARLETLIKFEKAYVYMLDKQKRTID"
     gene            complement(5934..6272)
                     /locus_tag="IXT53_001925"
     CDS             complement(5934..6272)
                     /locus_tag="IXT53_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905729.1"
                     /translation="MFDSIKNLVSGMTPDMKTLVQVVLYFLAFVFSLKPGFTAMKFFG
                     DAKWGDGLKYVGAIIAIFIIPVITTVMLYTMGTKTGNDLNQKANIISALIPVVGIYLV
                     SKFPKNELLG"
     gene            complement(6672..10313)
                     /locus_tag="IXT53_001926"
     CDS             complement(6672..10313)
                     /locus_tag="IXT53_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905730.1"
                     /translation="MKFKKYLLQNLRIKRWVVRGLVIGGVLAVTLGAPFGMNDPTPDN
                     QDSKQETVINKNVAYAESKPAEQGMWTLYARILMGDKEVNDSAAKTGQMNLLGQGGIS
                     ADFPYSDISKQGNSIAGGNAGTQLASTLATYSYYGYIETVSGNAIASQASSILGSIGR
                     TIGSFIGSISLILSNIAESINELLGKFFMSMNIFSLMGWGEGGSDVSNPVSKALTSLL
                     NNLGLNNELFKAIASLSMLFFTAILGYQGMKLLSKDGVNSKGLSPVLEKWIVRIFVLF
                     IFLPVAGSLLGVYINGIQSAKKDGDIPTNVVSDYIINTRLWASTQNLAPSSDTGGDVP
                     DGKPTSEIKFVDTDYEPSKKSAMIRAINTSSYARQNNDLDSNSVGMALLKEWSSNDTF
                     NINTYAGDVNVAENDAYMGRAPAVWTGYNLGDGKEGSAIKKPTNLNGYIWSANQNVTD
                     DNRKPDGKGTAHNFDATQPWGVANNATFSTQSVALLLQSALDTSGAKFYAYNIAPSGI
                     QGSAKNMSTVKTEWRTASLVGHGAMGKFGSWLAMNAESIVLAVLYLACAWAMFNVNIV
                     KAFKREFIRIWEVVLTGSLASGAGVMAISLGVVTTSLIGLQLPIFLITFIRAFGDTAV
                     GFIPNTLVGDGVANIVRSAMILGVGYLLVFKKGEKVNNLINMLIGLPLEVAFGFDERA
                     KLLFGNRNDAVRVVDGVSKAIKKSQGAKNPYYDGGNSSVKDMVAGGLGAVGAQKMAER
                     LNNMGDSNADAKSIADKVIPETPEGMSFNGGGVGSTINNTSFKDLVRQDQLRNNSQQY
                     IDHLGRMVDENGNFVDENGEILKDSKYGKDAKGNFIDEKGRLVDEDGNLIDTNGKPLE
                     DSEDGNIGMDSEGRLIDEKGRLVDEDGNLVDENGRRLLNGDFGHDSNGRLVDEKGRLV
                     DEDGNLVDEFGNKLGKHSDGYNVNGDLVDEDGNIIETRDALVADRNRELEQQRHTPIA
                     VAIPENEEDEITLDQTHQELRNEMSGEDVSDRVHRELGDGASGESQSRELNSNFDNSA
                     IRNDTVLKDRESLTKETDKEQSDVKKKFIPTPNVNQTDKYETNIGSGMSKDNQSIREP
                     ELTNKNNRTNNSKSTSDNSGKSNQSTKKPSTAKSAVEYLSKTGKNVYTSADKALAKAP
                     KGSLAGITYEAKNEAIKIGKGTAKVAKATATGASKTGKVVKKSTMSVKDYIVKYGNQK
                     STENKSEDE"
     gene            complement(10316..12265)
                     /locus_tag="IXT53_001927"
     CDS             complement(10316..12265)
                     /locus_tag="IXT53_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAQ4905731.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKAEDKDNEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQM
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFR
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            complement(12307..13365)
                     /locus_tag="IXT53_001928"
     CDS             complement(12307..13365)
                     /locus_tag="IXT53_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HAQ4905732.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLEERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVNKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            complement(13484..13939)
                     /locus_tag="IXT53_001929"
     CDS             complement(13484..13939)
                     /locus_tag="IXT53_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905733.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            complement(14523..15326)
                     /locus_tag="IXT53_001930"
     CDS             complement(14523..15326)
                     /locus_tag="IXT53_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAQ4905734.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKALSDKLGTLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
     gene            16306..17529
                     /locus_tag="IXT53_001931"
     CDS             16306..17529
                     /locus_tag="IXT53_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAQ4905735.1"
                     /translation="MESYQFQIFKLVPSLERRGNRMKITEKNVNPKFEPTESNLKKFD
                     LTVTLSNQLVDSIVEMDAMPQKIYLYAIGHLDPKNPPFNNTVKVSKKALFALMSSSSS
                     SSKHSRLKQVIEKLHQQSVIKIQRTKPNGKTEYKVISPLEITEFNDFSDAVSIKFSSS
                     IMPLLTNLEDHFTQYTFDQVVNLTSKYAISFFQLFTKDFNLYEANRDNLTWSTKSLDR
                     LKNPVFSIADLRKKFSIEKKYKAFRDFERRVIKEPIAQLNKYTDFKIDYELIKKGHTY
                     TNIKFLLTRTMSVPVPYKQEQRDPAYLKGAEDKTEKMNQSYLKAMNSKYTKLLIKNFL
                     ISSADLCDLKLMSRLQNKVYPLYDRLSKDRGFESVVNHLSYVATHKEDYTIKNTVKYL
                     EKSITSYLPYVNLYD"
     gene            17564..18331
                     /locus_tag="IXT53_001932"
     CDS             17564..18331
                     /locus_tag="IXT53_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAQ4905736.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPTKLKKT
                     GVVDLPVKAIINYMDVDQNLETELKTIVNTLQRQAVYEISNLDKKYKTYTTVSAIDRI
                     HWDLEEHSVSFSFSEDVIPLIVSIKKKYPFFVFEDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LNIDFAKVRKGRNYNFAVQFNISSEFDEKTYREKKLG"
     gene            18523..18921
                     /locus_tag="IXT53_001933"
     CDS             18523..18921
                     /locus_tag="IXT53_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin HicB"
                     /protein_id="HAQ4905737.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
BASE COUNT         5732 a         3615 c         2754 g         6867 t
ORIGIN      
        1 aactctcgct ttttcttcat cagtaaagtt tttatacaac tgattacaat aatgaggttt
       61 ttgaagtttc tgaataccta tactaacttc aaatccttct tcatgttgaa ttaggattac
      121 ttctgtgtct ctacctagaa atattcgatc taaaattttt atgtttggtt tcattattta
      181 acctccacta taattatttc actcagtaag gtttcacttt atgaaatttc tatagttctc
      241 ttttcacaaa acgtaccatg ttctctaaga ctgtttttag tctcaaatat acatgttttt
      301 tcttatcctt tccttataca ctcaaaatca aagataaaac ttaaagaata aactctccaa
      361 taacaaaatt attagtcaga tccactggtc tcctgatctg aaatttatta cttcttcctg
      421 ttgtcaaata ctgtctcaaa tcaacagaaa gtttttgatc atcttctcct tttctagcag
      481 gagcaacttt tttttcatac ttcattaact ctccactggt catctttcca atgatagtgt
      541 aattaaaatc actttcaaaa ctttggtaaa tttttccatc tgtattgaat atatcagctg
      601 tttcaatatc ttctttgtat tcattgtctt ctcctgaacc gattgtatca aatagaaagg
      661 caagacgaat tccatcttct tcaagcattg ttaatctttt tttaattagc ccaacggttt
      721 caatagataa cttatcgaag ccgtgaatca taacaatatc atcttttttt gcttgtgatg
      781 tgatgaaatc aaaggtattt aaaaattgag cttccataac attggtttct gctcttagat
      841 cacctaagtt atagtaaaac tgtaatttgt cacttttacc gatttctttc agtgtcgttc
      901 tttgattaaa catcctagag aagctgtcta atgctgttct caatgtgtct tttaatacaa
      961 taatacgatc gacttctttt tcagtaccag attttttggc ttttgtttca gcattagtca
     1021 aatagctaat aaaatcccca agaactggca ttgtatcact actgttcacc aaaagttttg
     1081 aacgttcttc ataattaata tcccaaagtt tctgacttat ataaaagtta cctaattctt
     1141 ttttcaataa aactctagtt gcagaatcta atgttctgcc tgctaacaaa taaaaaatct
     1201 gagttagttt atcgagattc cgtctatata aatcaccaat gtccttttga ttaccaaaaa
     1261 gctccaaaga gttaagacct tcttcagcca aattaacctt ttctattaca ctgtttaacg
     1321 aaggtttggc aacaaatgtt ctaccagttg gatcaccaaa gtaatcaaat ttatttaaga
     1381 caatatgaaa agttcgttta tgatttgcca ttgcattatt agcaatcaat tgcccccaaa
     1441 gtgatgatgc tgagattttt tcatcatagc ctttaattgt agcgtttttg tgtgatgcca
     1501 ctaaaacttt acttgtaaaa gaatcatcca gtgccataaa agctacacca cttgcgtagc
     1561 tagtagacat agtgccgata gcccatccat tcgcatcatc taaacctttg cgaattgcat
     1621 gatcatttcc tgcaaaatcc gaactcatag acgtatactc atagacattc ccgattggtt
     1681 cacttaaaag tctatcaaac agatcacgtt gatctcctcc atatgacata agctttaatc
     1741 catacatttt ttcatcatac aaccgttgga gtttaattag ctgttcgctg attttattag
     1801 gagaatcgga tactaatagg atttctagct tccaatcaat aattttattt gccatgctgt
     1861 ctagtaaaga tgctgtattc aaatccataa tccgcataac agttttgcta atttcagcat
     1921 ctgaattttt tccttctaaa ttattttcat aactattcgt tgatccccca acattctcgt
     1981 gcattatttc ttgttgatct ttttgtgaca ctggcttatc tgcttcaatt agataggctt
     2041 ttaccccttc tactctcact tcaggaatag ctttaaccat ccaaccaaat ccataatttc
     2101 tattcattcc ataagtattc actaatttca caattgttcc ccaccgctta cctgtcctaa
     2161 tgtaagatgg tgtaaattga taaccaacta aagatgttaa atcactaaca aaggtattat
     2221 tcactacctt tgaatttcta gttttttgtt cttttttagt ttttctcttt tttttgaaat
     2281 ttaatagttg tgatctaccc ataatttcca tttaatctct cacctctatt tcagtgcata
     2341 aatatcagat aatatttctt ttgtttgttt agctgacaac tgagtgacac tataataaag
     2401 tcctctactc gtatagtttt ctaaacgttc cacaccttca tccaatacca tcttagattg
     2461 agatttcaag ataagatatt ggattactgt tgttttcaca ccatcaatat attctgatac
     2521 aaatcgttct tgctgatcta ttatgtcaaa gactgctttg ttactccttg catttcgttt
     2581 ttgtattttc atatttcgag tttgtctttc cgtattctga ggttgagatg aagtgatttt
     2641 gatttcagct aaggaacgat ctctggtatt ttgatattgt attgatttcg attcctgagc
     2701 cataacttct tctggaaaag cggttagtga tgtagcccca tctagattga acattaatgc
     2761 tacactccca tctcgatata cgattgttcc atcttgtttc acttttaaaa tctctgtgcc
     2821 tcgaaatggt ttctttttcc ccatagatac tcttgctgaa ttaactactg atttgaacag
     2881 tctaatagaa ccagaaatca atgaaccact agatggatca ataaacatat actggctaat
     2941 tagaataaga aatccaatta tcattaataa cgttggaaca aaaataaatt tcagtttaat
     3001 cgtatcagaa actaagtaaa caattgatcc aagaacaacg actgcaacaa ctatagctga
     3061 tacaattaat ctttttgctg taacagaata ggtgctattg acatgctttt caagccattc
     3121 aactctgaca attgtttcag aaggtttttg atgtaagtta tgtttaaatc catagtttga
     3181 gttatctgcc atcagaaata cttcctttca cgttcaactt acggtaaaca cctacagtta
     3241 tcactattgc aacaataagc aagccaatta aagatattga cttgctcatt gtctcgttcg
     3301 tttgtggtag ttcatttacc tgatacacga gctgtgcttc ctgtaagtgc tgagaagagg
     3361 tcatgttttc ttgattgttt tgaattactg gataaactat tttttcttct cgcataatag
     3421 ccctccttag ttattatcag ataagtgagc ttgaattttg tactgatttc cgtctggagt
     3481 tttcacaact gctttttctc catcaataga aatcacttca gccttagttg ttttcccatc
     3541 aatactgatg acaacatctt tcccaacttt gaactctgta gttggtgttg cttcatagct
     3601 actatttgtt gaagcagagt tcgtatttgt cccaatcgaa ttttccactc tcgaaataga
     3661 tgtttgagtt tgagcctgtg cttgttgatt attatgacta ataaagtagg acgatcccaa
     3721 gatcgttccg caagccattg cagcgacaga aatgatagta gccatctttt tacttgtaaa
     3781 atcacgtttt gaacttgatt gattttctgt agcaactgct aatgatttcg ttttttctgc
     3841 catttgagct agatcacttc gggaacgaga taagtcttct cttaaagctt cactttgttc
     3901 tgccaacttt tcgctattta atttataacc ctctgctaaa tctttgacct ctttcattcg
     3961 atctttatat tcttcgatag ttttttgttg ctctacaagt tttgtttcta attctttcgt
     4021 gttgttagct acctgctttt caacttcttc tttgttcgca aaatatttga caaccacatc
     4081 tgttgttttc tcttttgtat catggaactc agcttcattt tctgactttg ttttttcgac
     4141 aatctctaaa gttttttctt gcacgtctgt taatgatttt tcaatttctg gtttcaatga
     4201 attgataatt cgttccacca caacttcttt tgcttcttgg ctttgtttca atgcatcgtt
     4261 tttaatagat tcaattgctt cttggtactg ttgatcaaga ctttcttcag ttgcttttct
     4321 atattcatct tcttgttgtt taacttctgg taaatgctca gcatcatacc gttgctctag
     4381 tttctttagt tcagcatcca aataagcctg tttcgccata tcataatctt tacgaatgat
     4441 attggatttt tccatcacta aatcatcaat tttagatgct tctgcttcgt agctctcttt
     4501 agcaaccgat agtgtatttg taaactgatt ttgaccattt aatgaggttg cttcctctat
     4561 ccgcaaaagc tcatcagcta acatttgctt tcctttgagg atcaattgct ccttcgtctt
     4621 atttaattca ctgttgattt tattattagc aatagagatg cgagcgttca tactctcttt
     4681 agctaaaaac agcaatggtt tcaactcagg tatggatagc atctcctcgt ttaaatcgac
     4741 agggtcatta atctcatcta aaactacagg ttgaaatttg tcagcaatga tctgttcaaa
     4801 atttttttct aaaaaatcag catctaagtt tttcggtaca tcactttcag atgtgatttt
     4861 ttcagcttca taaacataat tttctttgtc actttgggat tcgggttttt catcaatttt
     4921 tggttgttgg taggtttctg tttgtatttg tgtttcttct atgcttactt cttggttttc
     4981 tgcttcggtt ggtttgttat catccacaaa aagagcttct ttttcatctg gtacattttc
     5041 tgctacggct tcaaattcaa cttcttcaga agagttgaag gtctcagcaa ttcgttctaa
     5101 tccattagta gctacttcta attgctctac tgcgctatag aagtctgtag cttcttcaac
     5161 ttctgattca aacacattag gatcatccgg aataactaat aattgaccac tactatccat
     5221 aaaacggaca cctgctaata gcttctcttt aaatttttct ggtagatcag ctacataatt
     5281 caaacttgac ttcaatagaa cgacatactt ctcagaatcg agaatagcat catcctcatc
     5341 tataactccc tcactgttga gcttttgtac atcttcaatt accatataat ttttctttgg
     5401 taaatctttg gtcagcaacg catcgaattc tttccatacc tttgctttag ctttttttgc
     5461 cttcgcttct ttttcttctt ttttgtacac ttcgagtaat tgtggcttca aagagagtgc
     5521 aactacagga actgtttctg ccacataatt aatagattcg gtaatatctt tgaattttgc
     5581 cattattgta aaactccttt tttcagtcaa tagttctttt ctgtttatct agcatatata
     5641 catatgcttt ttcgaattta ataagcgttt caagtctggc tttttcaatc tttttactcc
     5701 catttcgata atcatagaac tggttaacat tcatattcat tatctttgcc agttcggtac
     5761 ctttcagact gctcattaat acttcgttgg cgtgagctaa cattttttct ctatcaaaaa
     5821 ccatcttaca tttactttct tccctcactt ctaaactacg cttggagcgt agttttttgg
     5881 taaaaagtaa attgaacaaa tcggcatatt atccaaattt gttcaattca cttttaacct
     5941 aacagttcat tttttggaaa ttttgaaact agatagatac caacaactgg aataagtgct
     6001 gatatgatat tagctttttg gttcaaatca ttacctgttt tagtacccat tgtgtaaagc
     6061 ataacggttg taattacagg aataataaag attgcaataa tcgccccaac atattttaat
     6121 ccatctcccc attttgcatc accaaaaaat ttcattgcag tgaatcctgg tttcaacgaa
     6181 aaaacgaatg ccaagaaata caaaacaact tgtactaaag ttttcatatc aggcgtcatt
     6241 ccgctaacca aatttttaat agaatcgaac atataaaaac ctccttttta atatttttaa
     6301 taagttaatt atatcatcga caaatcttat ttgttcactt gtcaatgatc taactaagaa
     6361 aaagacgtct cgatatttac atctttatgt tatcataata acataaattt tcgatgatta
     6421 aaagagattt tccattaaat tatttcactt tttttattgg ctaaaaatgc tggtttatca
     6481 agtttttgtt tagataataa ttctgttctt taccagcata agcccttttt aattatacaa
     6541 ctttgaacag taaatattta attcatttat agacaattta aaataatttt tttgcataaa
     6601 tagaaatttt ccttgacatc ttcagttaga aatcaaagta aaaagaagga gcaaatcaat
     6661 gctccttctt tttactcgtc ttcagactta ttttcagttg atttttgatt accgtatttt
     6721 acaatatagt ccttgactga catggtagat ttcttcacta ctttaccagt tttggatgct
     6781 cctgtagcag tagccttggc aaccttagcc gttcctttac ctattttaat agcttcgttt
     6841 ttagcttcat aggttatccc tgctagacta ccttttggag cttttgctaa agctttatct
     6901 gctgaggtgt aaacattttt accagttttt gatagatatt caacggctga tttagctgtt
     6961 gatggtttct tggttgattg attagatttc cctgaattat ctgaagtaga tttagaatta
     7021 tttgttctat tatttttatt tgtcagttca ggttctctaa ttgattgatt gtctttcgac
     7081 attcctgatc caatatttgt ttcatattta tctgtttgat taacattagg agtaggaatg
     7141 aactttttct tcacatctga ttgttcttta tccgtttctt ttgttagcga ttctctgtct
     7201 ttaagtactg tgtcattccg aatagcacta ttatcaaagt tgctgttaag ctctcttgac
     7261 tgactttcac cagatgctcc atctcctaat tcccgatgca ctctgtctga cacgtcctca
     7321 ccactcattt cattcctcag ttcttgatga gtttgatcta aagtaatctc gtcttcttca
     7381 ttctcaggaa ttgcaacagc aattggcgta tgccgttgtt gttcgagttc tctatttcga
     7441 tctgccacaa gcgcatctct cgtctcaatg atattcccat cctcatcgac taaatctcca
     7501 ttcacattat atccatcgct gtgtttacca agtttattac caaactcatc aacaagattt
     7561 ccatcttcat caactaaacg acctttttcg tcaactaaac gtccgttact atcatgacca
     7621 aaatcaccat ttagcaacct gcgtccattt tcgtcaacaa gatttccatc ttcatcgact
     7681 aagcgacctt tttcatcaat taaacgtcct tcgctatcca taccaatatt accatcttca
     7741 ctatcttcca atggtttacc attagtatct ataaggtttc catcttcatc gaccaaacgc
     7801 cctttttcgt caataaagtt gcctttagca tctttaccgt atttactatc ttttaatatt
     7861 tcaccatttt catcgacaaa gtttccgttt tcatcaacca tacgtcctaa atgatcaata
     7921 tattgttggg agttattacg tagttgatct tgacgaacta aatctttgaa agaggtatta
     7981 ttgattgtgg aaccaactcc gccaccgttg aatgacattc cttctggtgt ttcaggaatt
     8041 actttatcag caatggactt agcatcagca ttgctatctc ccatattgtt caaacgttca
     8101 gccatttttt gcgctccaac tgctccaaga cctcctgcaa ccatatcttt tactgagctg
     8161 ttgccaccat cataataggg atttttagct ccttggcttt tcttgattgc tttagaaacg
     8221 ccgtccacaa ctcgcacagc atcatttcta ttaccaaata ataacttagc acgttcatca
     8281 aacccaaacg ctacttcaag tggtaagcca ataagcatat taatcaaatt atttactttt
     8341 tcgccttttt taaagactaa aagatatcct acacctaaaa tcatagcaga tcgaacaata
     8401 tttgctacgc catcgccaac taatgtatta ggaatgaatc ccacagcagt atcaccaaat
     8461 gcacgaataa aagtaatcag aaagataggt aattgtagtc cgatcagaga tgttgtaaca
     8521 acacctaatg aaattgccat cacacctgct cctgatgcaa gtgatcctgt tagaactact
     8581 tcccaaatcc gaataaattc acgtttaaat gctttcacga tattcacatt gaacatcgcc
     8641 caagcacatg ctaagtaaag aactgctaaa acgattgatt cagcgttcat cgctagccac
     8701 gagccaaact tacccatagc tccatgacca actaaggatg ctgtacgcca ttctgttttt
     8761 actgttgaca tattcttagc actgccttga attcctgaag gagcgatatt atacgcataa
     8821 aacttagctc ccgatgtatc aagtgccgat tggagcaata aagctaccga ctgtgtgcta
     8881 aaagttgcat tgttagcaac gccccaaggt tgagtagcat caaagttatg cgctgttcct
     8941 tttccatcag gcttgcgatt gtcgtctgtc acattttgat ttgctgacca aatatatcca
     9001 tttaaattgg ttggtttctt aattgcactt ccttcttttc catcccctaa attatagcct
     9061 gtccaaacag caggcgctct tcccatataa gcatcatttt cggcgacatt aacatcaccg
     9121 gcataagtat taatattgaa agtatcattc gatgaccatt cttttaatag agccataccg
     9181 actgaattag aatctagatc attattttgt ctggcataac tgctagtatt gatcgctctt
     9241 atcattgcag attttttaga tggttcataa tctgtatcaa cgaacttgat ttcggatgta
     9301 ggctttccgt caggtacatc accaccagta tcagatgatg gagcaagatt ttgagtagaa
     9361 gcccaaagtc ttgtgttaat gatgtagtca gaaacaacat ttgttggtat atctccatct
     9421 ttttttgctg actggatgcc gttgatataa actcccaata gcgatcctgc cactggtaag
     9481 aatataaaca atacaaaaat acgaacaatc catttttcaa gtactggtga taatcccttc
     9541 gagttaacac catctttcga caataatttc attccttgat aacctagtat tgctgtgaaa
     9601 aagagcatag ataagcttgc tatcgcttta aataattcat tatttaatcc caaattgtta
     9661 agcaagctag ttaatgcttt agataccgga ttagatacat ccgatccacc ttctccccac
     9721 cccataagag aaaatatatt catactcatg aaaaattttc caaggagttc atttatggat
     9781 tcagcaatat tagaaaggat taaactaatt gatccgataa aactaccaat cgtcctacca
     9841 atacttccta gaatggatga tgcttgactg gcaatagcat ttcctgagac tgtttcaata
     9901 tatccgtagt aactataagt tgctaaagta gatgctagtt gagtacctgc attaccccct
     9961 gcaatcgaat taccttgttt agaaatgtca gaataaggga aatcggcact gataccacct
    10021 tgtcctaaca aattcatttg tccagtttta gcggcactat cattaacttc tttatctccc
    10081 attaggatac gagcatatag tgtccacatt ccttgttctg ccggtttact ttccgcatat
    10141 gcaacgttct tatttataac tgtttcttgt ttgctatctt gattgtcagg agttggatca
    10201 ttcattccaa aaggagcgcc taaagtaact gctaatactc ctccaataac tagacctctt
    10261 acgacccatc gttttattct aaggttttgt agtaaatatt tcttgaattt catagctacg
    10321 cccctccaat cgaaaataca atcttgtttt ctttactttc agcaacggaa ggctttgcaa
    10381 taatttcagc attagaatca ccttttcctt caaacgctcc ggcttcactc aacttaacat
    10441 cgaatatttt tgcgtttgga taagtaatta ttgcttcagt aatcgcttct tcaaactcag
    10501 attcaactaa atgcaagata ttccttgcac cccctgattt agaatcctct tgtgaagcca
    10561 tgtcaaaaac taagtgcatg gagagatcgt ctgcataaaa attttgacca tatactcttt
    10621 ctttcctagg tggaagacgt aaatctatat ttaaatgttt tcgtactaat tcagcactct
    10681 tcaacatttc attataagca atttccagaa gagtttgttc ggataaagcc caaaatggaa
    10741 ctattcgtcc aaatcggtta ataacttcag gctttagacc ttcaccaatc aaggcttgtt
    10801 tgatccctga tgcgactttt cggtattctc tttttagttc agtgctgtat tcattagaat
    10861 tttccttgtt tgtaacacga gatagtgagc tatatcggtt taacgaatcg aacatctccg
    10921 cacctgcatt tgttgtcgca aataaaattg tatttgacaa tgaaacggtg tcgtgaactt
    10981 ctcgatcatc ccatcctctt tcagtaaaac ttatcctacc ttcatctaaa attgataaaa
    11041 aggcatcgag tgtttctaca ccagctttct catattcatc acacacaata atcgcagaag
    11101 gatcatgcct tacatgagta cctataagtc gtctaaaacg cattgcatca tctttggttt
    11161 tgtagtccgg catattaact aagagaactt tattttcatc gccataaagt cctcttgcca
    11221 cggctttagc cattgcagtt ttaccgaccc ctgttgatcc tgtaaacaga gcagttgctt
    11281 ttggttgctt tcgaccttta aaacgatcaa atccaagctt catacgttta aacaacattg
    11341 ttatagtata aactgctaac ggttgtcctt ttatggactt taattcctct tgaagttttt
    11401 ccatatcaaa atcagaatct agctgaatat tatacttacc aaatatttgc aatagacttt
    11461 ctcgattata agcatttccg tctactcgat cttttgcaat caaatcctcc attacagcaa
    11521 ttgatttagc cggttcggac aaatcctcac gataaagttt gtttgcttgt acgatttctt
    11581 gaagtaattc atcaggaatc ctttgcgata atctaaaaga agcctgagga tcatctttaa
    11641 cagaggatgt atttagccat tgtctcaaaa tatcgacgac aacatcgctt tttagttcat
    11701 tcaattctaa cggttgtagc cgtctcccta aggcatcatc tcttttgata tagttttcat
    11761 attcttttct tgtagtagcg gtaatcattt gaagtgattc atttgctaac ggttctttta
    11821 gcgaatcacc cccaatttta ttgccactcc caaaagagct aacaacttta tgaaactcgt
    11881 caatgaagag gacaaattca ttatctttgt cctcagcttt taaaattcgc tcatattctt
    11941 tcaatcttgg aattaatgtt tctaaacgtg tttggaggac atcatctccc ccaacacgca
    12001 attgtccgat tgataattta tatacttgta ctttctttcc atcttgtcgc tgtctttcag
    12061 cccattcact tgcaagtgct gttttacctg ttccggcttc acctaacaag atagcttgcg
    12121 gtgtttccgg tctattcaag atcagtgaca actcatggag ttcgtcatca cgtcccacta
    12181 acttatgttt tggtggtttc attggtgtga gagcatgatt aaattcggtt tgaatattct
    12241 taaaaaactc ggtactattt tccatctgtt tgtgttcact tcctctaaaa taagaataat
    12301 ttacttctag tcagtcaaag ttaggtactt aaatgtcgtc ttcatgtctc caaaagaatc
    12361 tttgttaaag tcagcatttt tgtattgatc aatcaatttt tgtgtatcat tgattgcttt
    12421 aatttcggtc tccattcgac tgtctagttg acgaatactt tcttgcgaat ctttatcgtc
    12481 aggattttgt tgtaaacgtt ccagatactc agcttttgtg gcttcaattt tcttgatgtt
    12541 agtatttgaa gcatttaact gatcttctag cttatttaca acattattct tacttgtaac
    12601 tgctacaact tttgaataat caatatcgcc atctttggta agaaagcttc cttgatagac
    12661 attagttgaa tctgaatatg gatttattct gaaattgata ttatcaggag tattaacagg
    12721 ttttgctttt ttcccaaaag aaattactcc ttcattatta acatcattca tagaatattg
    12781 agaaagtgct tttgtaactg aatctgcttc attcacattg actttggtat catcgtcagc
    12841 tgtatcatca ccagaaatta gtgatacttg gtttgcaata aaaacttgat aagctctttc
    12901 ctctaattta ccttttagga ataagaaagc atccccatca gtacttaaca tcccatagct
    12961 catttcaagc ttcttaggac gatctttttc ttttgttgcc atatataagt tatagttttt
    13021 tccttgcgta gataacaact ttctagcgtt ttgggagtaa ccaagtttta ctactgttac
    13081 atctctattt tgatctgtcc acacatctga aatagtgatt tctgctcctg atcgagcaaa
    13141 ctccaatgaa gaccctatag gtgttgtatt gttcacgaga taataattat agtttcctaa
    13201 ttgataaatg tagctaccca agcctgttaa taaaaaaagc actcctagaa aagttgcaag
    13261 cataatgcgt aatccgtgac gtgttctctt atccttatca ccaagcaacc ctccaataat
    13321 actgtattga tctttggtag atgttgaatt gacgtttttt tccatcagtt tgtctgcccc
    13381 ctttcataaa agagcaaaca atcagacgtt ttttcggtaa ttctaggttc gggattttga
    13441 atcatttcgg tatattcttc cccattaata tttaacacaa aaattatctt cctttcttcg
    13501 ttcctttctt ttgctcttta ttttccaaat aatcaatgat ttttgctatt gtaccaagat
    13561 agtaattccc ctcagctttg ttttttcgga ttttgctaac cattggagta ggtacaatct
    13621 cttcatctct caattcactg aaagattttt tacctagata ttttccaata acagatttta
    13681 aaaaacgttc aaaatctttc tcgtaagttg ttttcttgcc ttttagttca tggttcatat
    13741 tgtcaacgac ttttgcggtt tcaataattg ttttagttct cagcttaata atttcatctg
    13801 gaattttcaa tcctcgtaat ttcatcgttg tactaactgg taacccatgc tttactgcat
    13861 cagcaatact aaaacttttt agatcatcta gtaagctctc catatagtta acaaattcat
    13921 gccaattaat agcattcatt gtctcacccc ttcatttttt taaaatgagg ttcgggcgta
    13981 acacatttat gttattataa tatcataata acataaataa atcaatcagc aaatttagat
    14041 atcccttaaa gtatcagcaa ttattcaaca agatatttat tttagctatt tcagtcatca
    14101 taaaagacta attagcaata aaataactaa tattatccat ttatcagacg aaaagaaaaa
    14161 acttatctaa ttactctata ttaactttta acaaatagtt gacttgtcca aaaatgttac
    14221 cttcaatctc acaaagatat taagatgttt cctaccaaaa ctgatatcac cggacttttt
    14281 attctgtgtt gtccaaaagt gttaccttta gtcgatcaac aaattaaaaa aaattgtttt
    14341 tttcttgact tgtccaaaag tgttacccat agttgttaaa atttgtatag attagccatt
    14401 ctttcgaata ttatttagac tgaaacttgc ttacaaaaaa acttcttgca tttattgtcc
    14461 aaaagtgtta cccatacttt tttaaagaag caggttgtat taaaattaca acctgcttct
    14521 tctcaatctt cttctaacca attatgcatt gaaatggttg gcaagtttga ttctggctca
    14581 ctaaaagtga atctgagccg atcaactctt cttcctttct ttgcatagat tttttcataa
    14641 tcaaaagttt caaatacctg tattccgttt tcatcaggag ctaaaaattc ttctttcgcc
    14701 tgctctagta ttcttttgtc aatatttgtc attctataac ttgaaggtat acttaatgct
    14761 cttctaaaat cttcaacttt gactacccaa taaccatttc gccactcttt atttctgtat
    14821 tgcatgagat gacgatacaa ttcttttgtg tagctacttc ttagttttgt aaaattatca
    14881 agttcaaaac gagtaaagtt tttgccaatt gagtttaaga taaattcaaa ttcctcattt
    14941 gccttaattg taactgtttc cttctgttta tctatccgga atttttgaaa aagccacagc
    15001 tgttcataga cgtctgaatc ttcaaaacgg taggttaaag tacctagttt gtcactcaaa
    15061 gcttttaaca cagaataaaa actgtccttt tctcttctgt cgtatttgac caaataacga
    15121 aagaggttga aatcaaaagt tacttctgct gttccttgac gtttcatttt tgataaaata
    15181 gcccaaaaca catccatttc aacaggctta aatcctcgca aagctgttgt attcagacca
    15241 ttgttataac gcacaattgc atttgtttgt tctgatacta aatctacatc actatcaata
    15301 atcttttcgg taacttcttg agacattgat tactctccaa tcttcttttt gcgctccttt
    15361 aatcatgtta atctggtact aattaaagga aacaattttt tctgcttgtt acctaaatta
    15421 aatcacaact aaaggtaaca gtcaacaaag atttgttact aatctaattt tctatttttt
    15481 cgccttccaa atcaagaaaa ccgaaggtaa cgtcttatcc tagtgtgata cctcaaatgt
    15541 ccacttttgt tacctttgct gtccaaaagt gttaccctta ctgtccactt ttgttacctt
    15601 tactgtccaa aagtgttacc tttatacccc tttaatactt actctcccaa gagattaagc
    15661 ctcccctaac agatatatta acagatattt aacagatata ttaacagata tttaataaag
    15721 gaggattact cctctcttat ttcaaaatct taaaaataaa aaatcttaaa aaaaactaac
    15781 actatctagg agtttatttt gcttgtgtaa ctttgaataa ttgaaaaaaa ggaaaaggag
    15841 gaagcttcag cttccccttt tatttatatt tataatatat attttaatct ttaaaacttt
    15901 agcaaccttc aaaaaggctt tatatcaatc ttaaatagat tttagggtgg acataaagcg
    15961 atgcgaacat ggacaaaaag cgatgtgaat gtggacaaaa agcgatgcaa acgtggacaa
    16021 aaagcgatgt ggtattccga atatatccaa aaaaagaaaa agtatgtgaa tttatttcta
    16081 aaaactagaa aaatatttgt tttttaattt tggtaatttt tataatttcg tatttttttg
    16141 aaaaggattt ttatattgaa aatgttctaa aatagcgatt tctgctgtct tatcaatgaa
    16201 aattgattaa aaaaataaaa tgtgttttac agagattgag ttatgttata aatttacgat
    16261 acatttctta ttgtatcgta tcgaaaacag gtctatactt attcaatgga atcgtaccag
    16321 ttccaaattt ttaagctcgt accaagctta gaaagaagag gcaatcgtat gaaaataaca
    16381 gaaaagaatg taaacccgaa atttgaacct accgaaagca atctaaaaaa gtttgattta
    16441 actgttactt tatcgaatca acttgtagat agcattgttg aaatggatgc tatgccacaa
    16501 aaaatttatc tttatgctat aggacatctt gaccctaaaa atccgccttt taataatact
    16561 gttaaagtat ctaaaaaggc tttatttgcg ttgatgtctt ctagttcatc ttcttcaaaa
    16621 cattcaagat taaaacaagt tattgaaaaa ttgcatcagc aatctgtcat taaaattcaa
    16681 agaactaaac ctaacggtaa gactgaatac aaagtcatct caccgctaga aattactgaa
    16741 ttcaatgatt tttctgatgc agtatctatt aaatttagta gttcaattat gcctttatta
    16801 acgaacttag aagatcattt tacgcaatat acatttgatc aagtagttaa cttgacaagc
    16861 aagtatgcga tcagtttttt tcaattattc actaaagatt ttaatctgta tgaagcaaat
    16921 agagataact taacatggag tacaaaatca cttgatagat taaaaaatcc tgttttctcc
    16981 attgcagatt tacgaaagaa atttagtatt gagaaaaaat ataaagcttt tcgagacttt
    17041 gaaagacgtg taattaaaga accgattgcc caactgaata aatacactga ttttaaaatt
    17101 gactatgagc ttatcaagaa gggtcatact tatacaaaca taaagttctt gcttacacgt
    17161 accatgtcag ttccggtacc ttataagcaa gaacaaagag accctgctta tttaaaaggc
    17221 gcagaagata aaacagagaa aatgaatcaa tcgtacttga aagctatgaa tagcaaatac
    17281 actaaattac taatcaaaaa ctttttgatc agttctgccg acttatgcga tcttaaactt
    17341 atgagccgac ttcagaataa agtttatccg ctttatgatc gtttatccaa agatagagga
    17401 tttgaatcag ttgtcaatca cctatcttac gttgctactc ataaggagga ttacacaata
    17461 aaaaatactg ttaaatatct cgaaaaatca attacgagtt atttgcctta tgtgaacctt
    17521 tatgattaac aactgaaaaa atgcgtgaag gaaggaaaaa cttttgaaaa aagatgaact
    17581 gacaaagtac aatgaggagt tcttagcaac aattgacatg aaaatggatt taaagtcgta
    17641 tgaactttat caattgctgt tcagttatat ctcacccaca aagctaaaga aaacaggcgt
    17701 agtagattta cctgttaaag cgattattaa ttacatggat gttgatcaaa atcttgaaac
    17761 ggaactaaaa accatagtta acactttgca aagacaagca gtttatgaaa tttcgaatct
    17821 agacaaaaaa tataagacgt atactactgt atctgctatt gatcggattc attgggattt
    17881 agaagagcat agtgtatctt tcagtttttc agaagatgta attcccttaa ttgtctcaat
    17941 caaaaagaag tatccgttct ttgtttttga agacttagca tcttttcaaa aaaaatatag
    18001 catcgtgtta tacaaatatt tggcgatgta ttataaaaaa tatcttatcg ccaaaaataa
    18061 aggagatttt gagaaagctg aaatatataa gaatcccctg attgaaatag ttaaaattcg
    18121 tgagattacg aaaacagaaa atgagtataa aacaatatcg aattttgata gacgtgtttt
    18181 aagggaatca gttgaggaaa ttaatgaaaa atcaatgctg aatattgatt ttgctaaagt
    18241 tagaaaaggc agaaattaca attttgctgt ccagtttaat atttctagtg agtttgatga
    18301 aaaaacttat agagaaaaaa aattaggtta atgcaaaata tatcttactc gtttgaagat
    18361 gcttcagacg tatgctaaga tatatagtgc cttgggagca aggtattaat ctcctttaga
    18421 ggttaattag cttgtgatcg cctatcaatt tttttggtag gcgattcttt tttttgaaac
    18481 aaaaaagcaa aaattatttt ttaattagag aggtgcaatg cgatgaaata ctttttttgt
    18541 gcagtattcg aatggaaagg aaatgaatat gcagttaaat tcccagattt tccagactta
    18601 tatacacatg gtgaaacttt agaagatgct tttgagatgg cgcaagaagt attggaagga
    18661 tacttggtgc ttttagagtt gcaagaaaaa gaaattccat cgcctacgcc tgttcaagag
    18721 ttaaatgcaa gaatcaagcg atctgagaat gttaatttat ttgtcgtaaa agctaatacg
    18781 aaagttgcta aagctcgaaa caagcaaaat tatgatcgaa aatcagtgac tgtggaacac
    18841 tatttaaatg ttttagcggc tgaagagaat attaatctgt cgaaagcttt gaatgatttg
    18901 ttgagagaaa agttgatttg atgtctctca gatgatcata ttaagcaaaa acagcttgtt
    18961 ttaggacg
//
LOCUS       DACGLW010000056        18942 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.058, whole genome shotgun sequence.
ACCESSION   DACGLW010000056 DACGLW010000000
VERSION     DACGLW010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18942
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.058"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            complement(21..251)
                     /locus_tag="IXT53_001934"
     CDS             complement(21..251)
                     /locus_tag="IXT53_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905738.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            complement(687..1253)
                     /locus_tag="IXT53_001935"
     CDS             complement(687..1253)
                     /locus_tag="IXT53_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ4905739.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1253..2209)
                     /locus_tag="IXT53_001936"
     CDS             complement(1253..2209)
                     /locus_tag="IXT53_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HAQ4905740.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2209..2865)
                     /locus_tag="IXT53_001937"
     CDS             complement(2209..2865)
                     /locus_tag="IXT53_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HAQ4905741.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            3235..5253
                     /locus_tag="IXT53_001938"
     CDS             3235..5253
                     /locus_tag="IXT53_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ4905742.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     gene            5680..8094
                     /locus_tag="IXT53_001939"
     CDS             5680..8094
                     /locus_tag="IXT53_001939"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016183651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HAQ4905743.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8442..9995
                     /locus_tag="IXT53_001940"
     CDS             8442..9995
                     /locus_tag="IXT53_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAQ4905744.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10376..12160)
                     /locus_tag="IXT53_001941"
     CDS             complement(10376..12160)
                     /locus_tag="IXT53_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4905745.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(12175..13896)
                     /locus_tag="IXT53_001942"
     CDS             complement(12175..13896)
                     /locus_tag="IXT53_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4905746.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13996..14757
                     /locus_tag="IXT53_001943"
     CDS             13996..14757
                     /locus_tag="IXT53_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ4905747.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
     gene            15724..15992
                     /locus_tag="IXT53_001944"
                     /pseudo
     CDS             15724..15992
                     /locus_tag="IXT53_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            16664..>18942
                     /locus_tag="IXT53_001945"
     CDS             16664..>18942
                     /locus_tag="IXT53_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905748.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTGLTTGEDGKLAIDGLAP"
BASE COUNT         6222 a         3361 c         3798 g         5561 t
ORIGIN      
        1 ctcattaaaa agcataacca ttactccgat ctgtttttaa aattagtttc tacctctaat
       61 atatatcgag tttttatttt gccttcagag aatactgttt ctttttttgc agttacaggt
      121 tgtttatttt cggaaaaagc taatatagct aagattgaaa catccatctg gaatttatct
      181 tttttactgc tttgatcata aaagtctgca tattcatcac tgatattttt tctaataaat
      241 tcttccatca taaaaatcac ctcaaaacga ttatacgaac ttacgttctt gttgtaaagc
      301 gatatttgag gggcaaaaaa ggggcataat ttgactgata ttaagttagc tattgatata
      361 tgaatgacat acaaaaatat aaaatctttt aaaaatgctt atatatcaat gtttttgcca
      421 ttcttttata tatctgttat atgtcaaaaa aatgtgttca atttcaattt gtatttgaat
      481 aaagaggttg tgaaaaagct gtcctgcatc aagtaataag aacagccaat caaaaacagc
      541 ttctcatgtt tttgatttgg ttggacttat taccgcagat ataagctttt gagcaccgtg
      601 aataaagagg ttatgaaatc aacgctctaa cctgagtatt agagaaaaaa acgcaaaaca
      661 tatcatttat gttttgcgtt ttttatttat ctttttttct caatacaata taaaataggc
      721 ggctggttct tttggttgat aaaccgataa ttcagcacat tatatgtttc ctgtggcagt
      781 ttggaacaaa aagaatcaac catatccagt tctttttctc caccttcatg accatagtag
      841 acgacaagga tcaatcgtcc gcgaggcaca agtcgtttca atatttcttc catagctgtg
      901 cgtgtagtct ctggcatagt gatgattgct ttatcacttt ttgggagata ccctagattg
      961 aaaatccctg ctttgagatt ttgttgttca gctatcactt ctcctaacgt ttcgtggcct
     1021 tgtagaaaca atgagacacg ttcttctaac tgacgctgtt ctagcctttc acgagtagca
     1081 tcgattgctt gttgctggat atcaaaacta tagacatgac cagtttttcc gatgtgttct
     1141 gctagaaaaa ccgtatcgtg gccattgccc atcgttgcat ctacgacgtg atcccctggt
     1201 tgcaagattt cttgcagtaa tgtatggctg aaatgcaaag cagtttgcaa catcagacgc
     1261 tcaccttttt ccgtacatca tattttcctt ggaagctatc tctgcgaaga agttcttcat
     1321 caattgcatt caagacttcc cattttttca agctccacat tggtccaatc aaggaatccc
     1381 acggcgcatc ccctgtcaaa cgatggatga tgatctcttt tggtatcatt tccagctggt
     1441 cgcaaatgac attgacatag tcaggacggc tcattaattg aaggcgacct tcatgatagt
     1501 cccgcaacat tctcgttttg cgcatcagat gcatcaaatg gagcttgatt ccttggatat
     1561 ctgaatctaa aatggtgcgt ctgacatttt ctttcatcat ttctaaactt tcgcctggta
     1621 atccgttgat caaatgcgta caaacacgga tattatgttt gcgtaattta gccacgccat
     1681 ctaaataagt ttgataatca tgtgctcggt tgatcagttt tgaagtttct tcaaaagttg
     1741 tttgcaatcc aagttctacc cataaataca tccgctcgtt cagttcagca aggtaatcaa
     1801 ctacttcatc tggcagacaa tccggtcgtg taccgactga taatcctacg acacctggta
     1861 aattgactac ttgctcaaat cgttctttga ttatttcaac tggcgcatgg gtgtttgtaa
     1921 aattttggaa ataaacgatg tattgttgga catgaggcca tttttgatgc atcatgtcga
     1981 tttctttctg gaattggatt ggaagagggt cttcaggtgc aacgatcatg tctcccgaac
     2041 cagatacact gcaaaaagta caaccgccat gggcaacagt cccatctcga ttcggacagt
     2101 caaatcctcc atcgatcggc accttgaaaa ttttcccgcc aaactcattt cgtaaagcgt
     2161 aattccaaga atgatatctt ttatttcctt ctgaataagg aaaagtcatt aaaagtcctt
     2221 ctttctgtaa gagttatcta tttgttttct tttttctcta tacactggat aagtcaggca
     2281 cagccaagaa agactgagta agtagccgcc gatgatatca gtaggaaaat gaacgcccag
     2341 ataaatgcgg cttatcccga cacttactat aataaacatc atcagcaatt gaagcaatgt
     2401 gcgtaaggtt cttttttgaa caaaagctgg aagcagaaat agtaaagaac cgtaaagaat
     2461 catactgcct gctgcatgtc cactagggaa actgtaactt gtttcaacta ctaagtgcgc
     2521 caatgaagga cgctctcgca tgaaaaatag tttgatcacc gggttgatga ttccagcaat
     2581 tacaataaat ccagaaacga accaaaatgt ttctgccctt tttttcttat accacatcca
     2641 taaagccgca caagcaaata aaatgatgac ggtaagagga ttaccgaatt tagtcataaa
     2701 gagttggtag ctgttcagat caggataaga agctctgaca atgcctgtaa accactgatc
     2761 gaaaccagaa agccacgaca gatggaactt tacgatccag cccaaaatga aaaacaggac
     2821 aagaaagaag ctaccagcca cctgaaatga tgttttatat ttcatacaat tcctcctttg
     2881 aagcaatgac ttattatagc atttttggtc cagcgaaaaa aacggtaaag agataaaacc
     2941 aatgtatgat tcctctttga ataaccaata ttcttataaa aaaaataact gtaatcggaa
     3001 agacattttt tggtaatgca aatgaaatgt tagggaaata aaatcagtgt ttttcgaaat
     3061 ccttttaaac aagaacgtag acgtaggaag aacttgaaaa tgaacagaag ttgttcatta
     3121 cggtaaatta caatatgatt acaaaaaaca acaaagctct taaataactt ttaataaata
     3181 ttaagagaag ttataattat taataataga taaattgaag ttggaggata gaagatggaa
     3241 aacattgcaa gaaaagaacg gagacgttta gaacagacca aacgttttcg aaaagcaaaa
     3301 cgaggggcag caatcgtggg aactgcgatg gtaggctgtt ctgtcgctgc cccattagta
     3361 cagccggttc ctgttcaagc ggatgaaaca cctgttcagt tcggcgcaag aatcaataca
     3421 gcggcattta tcacagaaat tgctacttat gctcaaccaa tcgctcaagc caacgatcta
     3481 tatgcatcag tgatgatcgc acaagcagtt gtagaaagcg gttggggcag tagtgcgtta
     3541 tctcaagcac cttattataa tctttttggg ataaaaggca gctatcaagg acaaaccgtc
     3601 tacatggata cgcttgaatt tttaaataat caatgggtaa ataaaaagga gcctttccga
     3661 caatatcctt cttttgctga atctttcaat gacaatgcct atgtacttag aaatacttca
     3721 tttggcaacg gttattacta tgccggtact tggaagagca acacgaaatc ctatacagat
     3781 gcgacagctt gtttgacagg gcgttatgcg actgatcctg gttacgcagg caaattaaat
     3841 aatatcatcg caacttacgg attgacccaa tatgatacac cagctagcgg aaacgcaggc
     3901 ggtggagcga ctacaggtgg caataatact ggaagtgcag gtagtactgg cggttcaagc
     3961 aattctggta atacaaactc aggttcagca acttcgacca cttatactgt ccaatctggt
     4021 gattctgttt ggggaatctc aaataaattc ggtatcacaa tggatcaatt gatccaatgg
     4081 aacaacatcc aaaacaattt catttatcca ggacaaaaac tgacaatcaa aggtagccag
     4141 gaaaatggct cttcgacgaa taattccggt aacaatacca actcttcagg aaatgctggt
     4201 acatcaaacg gaggacaaac aactggtgcg aaatacactg ttcaatctgg tgattctgtt
     4261 tggaagatct caaatgatca cggaattacg atggctcaat tgattgaatg gaacaatatc
     4321 aagaacaatt tcgtttatcc aggacaacag ctaatcgtat caaatggcgg ttcttctaat
     4381 gctggagcaa atactggctc aaatactggg aatacaagtg gctctagttc gtccagctca
     4441 agttcttcta atacagcttc aggaacgaaa tatacagtta aagccggaga ctctgtttgg
     4501 agcgtctcaa ataaatacgg aattacgatg gctcagctaa tccaatggaa caatatccaa
     4561 aataacttta tctacccagg acaacaactg actgtttcaa acagtggatc aacaaacagc
     4621 acgtcaggta atacaggttc gtcaaccaat acaaataaca gcagttcagt taatactggc
     4681 agcagttcgt ctcaaggcac ttataccgtg aaagcaggtg aatctgtttg gagcgtggca
     4741 aataaaaacg gcatcacgat ggatcagtta atcgaatgga ataatatcaa aaacaacttc
     4801 atttatccag gacagcaatt gatcgttaaa ggtggaactt cggcaaatac aaatacaggt
     4861 tcaactactt ctgcagcgaa accaaataca ccgaatacgt ctgctacgac aagtacatca
     4921 tccactggta atacgatgta taccgtgaaa gcgggcgagt cagtttggag cgtagcgaac
     4981 aaacatcata tcacgatgga tcagttaatc gaatggaata atatcaaaaa taatttcatc
     5041 tatccaggac agcaagtgat tgtgaagaaa ggttcatctc aatcaacaaa ccaacaatca
     5101 gttactggtt cgaaaaccta tactgtgaaa tctggagaat ctgtttggag tgttgctgac
     5161 agtcatggaa ttacaatggc tcaactaatc gaatggaaca atattaagaa caactttatt
     5221 tatccaggtc aaacgttgat tgtagctaaa taaaatagct gttcgaagaa ataacgattg
     5281 aattccacaa aaatatttgt tactataagg ctacttaaat atgacaacac ttgaaaagga
     5341 agtagtaaaa agactgaagt ttcagagagc gcatggctgg tgagaatgtg cacggatgct
     5401 tttgaactcg cctttgagtt gcccttgctg aatacccgag taagcaagcg gcggtgacgg
     5461 ccgttacaac gttcgaggcc ttttcactaa ggtaaaatta ggtggtaccg cgtagattac
     5521 gtcctataaa aagagcaatc tttttatagg atttttttat ttcttttgtg aagtttgaga
     5581 aaacaaagca aaaggaaata aaaataaaca gattctgccg gttttttcgt tatatttata
     5641 atagaaacaa gtgttttcaa tagaacagga ggacagcaaa tgagttacaa tcataaagaa
     5701 attgagaaaa aatggcaaaa atactgggca aaaacgaata ctttcaacac ccatgatgat
     5761 cctgaaaagc caaaatttta cgcactggat atgtttccct atccatccgg tcaagggcta
     5821 catgtagggc atcctgaagg ttatacggca acggatatcc tttcacgtgt gaaaagaagt
     5881 caaggataca acgtgctgca tccaatgggg tgggatgcat tcggtctgcc tgctgaacaa
     5941 tatgcgctag atacaggaaa cgatccagct gagtttaccc aaaaaaatat cgaaacattc
     6001 cgacgccaaa tcaattcttt aggatttagt tatgactgga atcgcgaagt gaatacaact
     6061 gatccggagt attataaatg gacacaatgg atctttacga agctttatga aaaaggtcta
     6121 gcttatgaag cagaagtagc ggtaaactgg gtacctgaat taggaacagt tatttcaaat
     6181 gaagaagtga tcgatgggaa aagtgaacgt ggtggctatg atgtagtcag aaaaccaatg
     6241 cgccaatgga tgctgaaaat cactgcttat gctgatcgct tattggatga tctagaatta
     6301 gttgattggc cagaaagcat taaagaaatg caaagaaatt ggatcggtcg ttcagtagga
     6361 gcaaatattg aatttaaagt ggctggtacg gataaatcct ataccgtatt tacgacacga
     6421 ccagatactc tattcggtgc aacttactcg gttcttgcac ctgaactaga tcttgttaga
     6481 gaaattacga cccctgaaca aaaagctgct gtagaagctt atatcgaaga gacagctaaa
     6541 aaatcagatc taaagagaac ggatcttgca aaagaaaaaa caggagtatt tactggtgct
     6601 tatgcaatca atccagttaa tggcaaggaa atcccaatct ggatcgccga ttatgtatta
     6661 tcttcatacg gcacaggagc catcatggct gttccagcac atgatgaacg tgactttgaa
     6721 ttcgctcaaa ctttcggact agaaattctt ccagttatcg aaggtggaga tgttgaaaaa
     6781 gctgcttaca cagaagatgg tacgcacatc aactctgaat ttctagacgg catgaacaaa
     6841 caagaagcaa tcgataaaat gaatgaatgg ctagaagaaa atggcgtagg gaaaaaagaa
     6901 gtcagctatc gcttacgtga ctggctattc tcccgtcaac gttactgggg tgaaccaatc
     6961 ccaatcatcc attgggagga cggaacagtc acagctgtcc cagaagaaga gctaccatta
     7021 cggttgccta agacaaaaaa catcaagcca agcggtactg gagaatctcc attagcaaat
     7081 atcgaggagt gggtcaatgt cgttgatcct gtaacaggta aaaaaggccg tcgcgaaacg
     7141 aatacaatgc cacaatgggc aggaagctca tggtattacc tgcgttatat cgaccctcat
     7201 aacaaaaaag aactagcaga ttatgaaaag ttgaagcgtt ggctaccagt tgacatctac
     7261 attggtggag cagaacatgc ggtacttcat ttactatatg cacgattctg gcataaattc
     7321 ttatacgata tcggtgttgt tcctacaaaa gaaccattcc aaaaactata caatcaagga
     7381 atgatcttag gcgaaaacaa cgaaaaaatg tcaaaatctc gtggtaatgt agtcaatcct
     7441 gatgatgtgg tcgaaactta cggtgcagat acactgcgca tgtacgaaat gttcatgggg
     7501 ccattagatg cttcgattgc ttggagcgaa aatggcttag aaggaagccg taaattcttg
     7561 gatcgtgtat ggcgtttgat cgttgatgag aacaataaaa tgcgtgaccg tatcactaca
     7621 ttgaatgatg gcaagcttga taaagtgtat catcaaacag tgaaaaaagt gacagaagac
     7681 tatgaaaatc ttcatttcaa tacagcaatc tcccaattga tggtctttat caatgaagcc
     7741 tataaagtgg acgctctgcc ttatgaatat atcgaaggat ttgttcaact actcgcaccg
     7801 attgctcctc acatgggtga agaactatgg gcaatccttg gaaatgatgg tggtatttct
     7861 tacgcaccat ggccaacata cgatgaagca gcacttgttg aagacgaagt agaagttgtt
     7921 ttccaagtaa acggtaaagt tcgtgcgaaa agcaatgttc cccgagactt aggaaaagat
     7981 gagctagaaa aagcagcatt agccaatgaa atagtgcaag aatatattga aggcaaaaca
     8041 gtcagaaaag ttattgcggt accaaacaag ttagtcaata tcgttgctaa ttaattggct
     8101 ctttgtcaat aaggactgat gagttgtgca aaatcaaatc tgggccagaa tgaacttcat
     8161 tctgtctcag attttttgtt atttcaaatt gattaattga ttgatcaaaa aggttttgat
     8221 tttcatgttc tcaaacacgt tcaatgtctt tttcttctga atggtcttgt aagagatctc
     8281 tgtaatcatc aaatttttgt ctattaatct tagtattttt tgatgtataa actacctgaa
     8341 attgagagta cataccaaat tcataaattt tttattagtg ggactagtga ttgtatggat
     8401 tttgacgcat aatgaatgaa gtatgagaaa aggagagaca aatggatcaa gaactattta
     8461 ttatcgaagt attatcagct aaacaaaaaa ttatcgagaa agcgaaaaaa agagcgaaaa
     8521 gaataatgtt caagctttta agcttagtat ttttctctgg tttgcttttt ttcggagctg
     8581 tatcgttcct atatcgccat atgagtgcag tagtccgttc agctgaaggg atgcaaaaat
     8641 taaataattt gtatcacttt tcgtggggag ttggcggaat aacaattgtt gtgcttattg
     8701 tttttcttct acttatttgg ctccgttacc atcaattgtc gcgtcatttc gatcaatggc
     8761 ttagtcaaag agcacaacag tttttacata ataaaccaac ttctcaagat gatgactatc
     8821 tttattttga gaagacttcg acgagtcaag cttttcggat acaaaaaagt gattgccagt
     8881 tattgctgag atcacttgat gatttaccta tctacttagg cgaaagcaaa tcaaaattcg
     8941 gaatgaacac attccatatt tttttactag aaaacgaacc cagatgggaa aaaagccagt
     9001 cttcgcctgg tggatacctg acaactaaga tgagtacatt agtcactggt tttttcctca
     9061 ttcttttagc tggaacaatc tattatggga gatacactca gccaaaaatg tcttctcata
     9121 taaatgctgc ttctcacatg acaatgaact caactaacga tacgcatata caaaagacaa
     9181 atgaagggtt gttagctgat cctaagcaaa aggtaaattt gacaacacaa aagataaatg
     9241 atctcaaact tgatacagag acgcactctc tctatatgac aacagatagt ggtgctaatt
     9301 gggtatttgt gccgattgat tcgcaatggt tgcgttttgg cgaatacacc ctgacaacgg
     9361 ggctgattcc tgaaggttat tggatggaca acacatatga tatttccgcc gatttctcat
     9421 ggtttatcta ttcttctggg gatgatgtac gtctgctgac ttcaagagat aatggtaaaa
     9481 catggcaaac gtctattatc acccaatcag ttcagccgat acgttatcga aaagcggtat
     9541 tttatggcaa cgatggtcta tttatctgta catcagccag tgggatgtct gcagaaactt
     9601 tgtacattta cactactatg gatggcggaa tcacttggat aaaaagtgga agtacaatga
     9661 tcaatcagcc agtacaaaat gtcagcttcg ttacaacttc tttaggtttt gtttcgacac
     9721 gtgaaagttt gttttataca aacaacggag gacgctcatt caaagaatca gttctgtcga
     9781 ttccggaaaa ctatcagaca ggggggatag atctttttca atcaccaaat gaagttactc
     9841 aaataagcgc gaatacgtta gagacaaaat tttatttgtt gaaaaaagga ccaatcgatg
     9901 cagggaaaat gtttgcctgc ctcttccgtt caactgataa cggggaaaca tggcaattcg
     9961 ttcagcaact atcgcaagta gaacaaggag attaaagaag agagattgtg agcctgacgt
    10021 tcagcaccga gtcacaagaa acaaattcaa aaaacagctt ctcatatttg ttgaatagtg
    10081 taactcgtga ttcggagttg acaagagcaa caagaagcga ttttgaagta tagcttctcg
    10141 tattttacaa gatcgtgtct tattgccgat aggttgtctc ttgaacaccg tttattcgta
    10201 aaaagggatt gtgacaagtc ttttgtcact acttaccgaa cgaaactgaa ggagctatcc
    10261 aaaaagtatc attagtatta ttctttgtgt ttctagtggc accactagta catttaaaaa
    10321 agctatcgat catggactaa tcatacatga tcgatagctt tttagataat gtttgttacg
    10381 ctatctgaaa ttgactatta taaagactgg cataaagcgt tggtgattgg agtagttcag
    10441 aatgtgttcc tttttcgacg atatcgccat tcttcattac aaggatcatg tctgcatttt
    10501 caatcgttga caaacggtgt gcaatcacaa aacttgtcct atcttttgtc acagcttcca
    10561 ttgcttgctg aatcaatgct tctgttcgtg tatctacgct agaagttgct tcatctaaaa
    10621 taacgacagg aggattcgct agaatgactc gagcaattgt caataattgt tgctgtcctt
    10681 gagataacgt gccgttttcg ctagaaatga tcgtatcata tccgtctggc agtgttcgaa
    10741 taaagtgatc acattgtgcg atttcagccg cttcgataat ttgttctctt gtggcatcca
    10801 ttttcccgta ggcgatatta tcagctaccg ttccttcaaa cagccaggta ttttgtaaaa
    10861 ccatcccaaa agaacgacgt agttctccac gtgaaagctt ggtaatatca agaccgtcga
    10921 attcgatctt tcctttattc agttcataaa aacgcatcag cagattgact aaagtcgttt
    10981 ttcctgcacc agttggacca acgatcgcta ccgtttgttt tggctttact gaaaaatcga
    11041 cttgattcat caatatttta tctgatgtgt aaccaaactg tacatttttg aaagctattt
    11101 ttcctttcgg ttgttctaaa agtcttgcat ctggaaggtc tggaatttca tctgtttcat
    11161 ctaatatctc aaatatccta tcgattgctg ccattgcgga ttggatcgag ttgatcacat
    11221 aagaagcggt agaaattggt tcagaaatct gattgatgta ctgaagataa gcttgtaaga
    11281 agccgatcgt cacaccacct gatagaacaa gcatagcacc gaataccgca cttatgatga
    11341 aggcgagttg attaatgaaa cgaatggccg gatagatagc acagttcata aattgagcac
    11401 gtttaaaagc gttgtattgc tttttattta cttcgtgtat agcagcttct gcatgagctt
    11461 gctgattgaa tgttttgacc actagattac cagctaagat ttcttccatt cgattattta
    11521 aaacacccag ttcatactga ttctgttcag aaatctgttt gtttttgttc gcaatcttag
    11581 ttgtaacaat tgaactgcca acgatcagca ataagacgag caaagtaagc ttgctttcga
    11641 tataaaacag catcagacca gcagcagcta ttgttacgac agaagtaaaa aattgattga
    11701 tacctgtcaa taacacttga gaaagttgat tgagaccggt agttgtccga ctaatgatgt
    11761 cacctacttg atgattgtca aaaaacgaca tcggtaatgt tttgaatttt tttgtgattt
    11821 cttttcgcac cctcaatgtg atcttttcgc ttaacgctgc cattgtccgt tcctgaataa
    11881 atgaagtgat gctgcttaag actgaacagc cgatcagcaa taagacaggg aacaacaatg
    11941 cttgtttgat atcactgaaa gaaagctgtc caatacctgt ttttcttatt tctgataaga
    12001 gatcatcgat cccgattccc atgatcaagg gcatggctac gactaatagg ttcccgaata
    12061 aactgcatag aaacaatccg taaaaatatt tatgttcagg tttgatcatt ttccaaaaac
    12121 gtatcaaagc acgcgtgttc aacgtttttt tcttttccat cgtatccctt ctttctatat
    12181 tccttgtgat tttgcaaact cctgataagt ttggcaatta gacagtagtt cttcatgagt
    12241 accttgtcca acgatttttc cttcatctaa aacaatgatg ttatctgctt gttggattgt
    12301 actgaggcgt tgagcaacaa tcacgagagt tttctccgac atgtgtttat gaagggctgc
    12361 tcttaatgct gcatctgttt tatagtctaa agcggagaaa ctatcatcga atacatagat
    12421 ctctgctggt ttgatcaacg ctcgagcaat acatatccgc tgtttctggc cccctgaata
    12481 attagaaccg ccttgagcaa caaagctgtc caatccgtca ggaagggtgt ccactagttc
    12541 ttctaattga gcgatcttga tcgctttaga caactcttct gttgaggcat attcatttcc
    12601 catctgcaag ttgcttaaaa ttgtcccgct gaataagaaa gccttctgag ggacataact
    12661 gatgtgggag cggatctgtt cttgagtcat ttgagtaaga ggaataccac caagggttac
    12721 ttttcctatc gtagggtcac taagtctcaa gagaagtttc gccaaactac tttttccaga
    12781 tccggtcccg ccaacgatcg ctgttgtttt tcctttaggt attgtgaaat caatatcttc
    12841 tagaactgga tcagagattt cagtatattt aaatgtgaca tgttcaaaag cagctgcatt
    12901 tgtctgatca aaggttggtt cagctactgt tttgatcgtc tcttcctcaa tttcgatctc
    12961 tgcttgcagt atttcttcta tccgtcgtaa tgaggcaaac gatttaggga taatgaccaa
    13021 taccatagct gcgatcatca agtaagcaag tgtcagcata gagtattcga ctacagcagt
    13081 cacgacaccg atttgtaatg ctccttctgc tacaagattt ccgccaaacc acaaaataga
    13141 agcataaacg attcccatga gcaaaaaagc aattggagtg acccaagcaa atattcggtt
    13201 cactttgatc atttgattag catagtcagc aaagctctta ttcgttcgct tttcttcata
    13261 tgattgatta tcaaaggcac ggatcatatt aatcccagta aagaattctc tcaatgtgat
    13321 ggtgatccga tccatttttg gctggatact caaagataaa ggtgcccctc gtttcatcag
    13381 tcgataaaca atccatacat aaaaaacaac agagatcaat gtaacccatg ttagtaatag
    13441 agaataacga aacatcatga gaatggtgaa aacgcaaata accggtgctg gtaagatcat
    13501 ttggcaaaat aaaacaatca tttgctggac attatcgaca tcattcgtca ttcttgtcaa
    13561 aagagatcca gaactaaaac gactgatatc cttcattgac agctgttgga atttacggaa
    13621 atatttctcc cgcgtttgga atccaaacct agcggaagcc aatgctgaaa agtaactaga
    13681 ggcaactgca gcgattgttc ctagaaacgc tacaagcagc atttcaactc caattttttt
    13741 tataactgag gcttcgccac ttgctatacc tgtgtcgatc agctttgcta ctaattttgg
    13801 tacacctaat gtaccggcga tttctaaaca gatcatcaca aaggtaagga gagcgagtcc
    13861 tttgtttttc tgtaagaatg tttttaagat agacatatta ttcctccttt tttcctgata
    13921 cttttcgatt ctataccttc aagtaacttg aaggtcaata gaaaaaagaa taaactgaaa
    13981 aagaggtgag aaaaaatgaa aaaaatgtat acgattggcc aagtatcgga actatttgat
    14041 ttgccaaaat caacgatccg ctattgggat gaacaaggat taatccgttc aagtagacaa
    14101 gaagagaatg attatcgatt gtttgatatc gatgatattt ttatgcttta tgatatcgtt
    14161 ttttatagaa aattagatat accgatcaaa cagatgaaga atttatatgg aaaaacattg
    14221 acggagttat atgagacact tgatgaaaca gaaagaagaa tccataaaga actggtggtg
    14281 atgaaacaaa aacaaaaaga gataagggag caaaaaaaac agttgaaatt gatgatcgat
    14341 acaaacgagg aagaatttcc tgtagaagag atcccttttg actgtatgat ctcaacggag
    14401 tttgaagata ttgtggaaat caaaaaattt ctacctaact attcaagctt tgggatgatg
    14461 agtatgccag cttccagttc acagacgatg tatggatttt ttatcgaccc tagtgaagta
    14521 catttattta ctcaagacgt gatttgggag aaaaaagata cggctgttta tcgtcgattc
    14581 ttattgaaat ccgagatgaa ccatgcggaa cgtaataata ttttagaaat cagagaaaga
    14641 atgtatgaaa aaggatggaa aacaggagaa gtgatcggcc agtatctatt gacgaatacg
    14701 gatgaaaaca atatcagaac tgaatattat catgcatgga tagaaatgaa aaaataggaa
    14761 atatgtgttt aagataactc gaaaatgatg gttgttttag ttataaagaa aaattttctg
    14821 gttcgattac gggtttaatg tgggggatta ttagtcgtgg atagatagaa ttttagtttt
    14881 ttagaggaaa tatgtcttta aaaatggaat ggttgcaatt cttagcgata tatacgatgt
    14941 aattgtcctc ttaaaaaagt atggaaaaat aattgtattg ggtgttatgt ataattcatg
    15001 gtgaactgag actaatgaga agaaaaattc atattgttat gagtgagatt gtatcagatg
    15061 agaatgataa attaaatagg tgcataaaag cattacactt aaaatacatt gttgcttaca
    15121 ttattacaat atgttaataa ggcaaaaata aatagatgga ttgaataaac atatttttta
    15181 atcaaatagt ccctactgtg actttcctca gctatcggtg atactaccga tagcattttt
    15241 ttgcttataa aataattata agtatttaga gatttttctt ctaacattct tcaaacaaga
    15301 aaaatcacaa aggttattgt tatttttata tattcttttt aagaatgatt gtttttttat
    15361 agaaatttta aaaaataaaa cacgttattg atggatagtt agtttgttat ttggtaatat
    15421 tatagaagtt gactggtagt gatttatttt tcgtcgtttt ataaaaatgt tattttttgg
    15481 aaaaattcac aaatatgtaa cgaatgtttt tctggaattt gctgtgttaa aaagcagtat
    15541 gactaatata aatactattg tcatttatgc aattttttat ggatagtaat tttagagggg
    15601 aatcaatgaa ggaagaatag aagggaaaaa tagttgcaaa tacgccagtt agcaaaaata
    15661 gcaatacatc ttcaagcttt aaaattcgag agaatcatct tggtagtgga atcaactaca
    15721 acaaaaggat taaaccaatt aatagataga attaccaatg gaaaaataga aaaggtagtt
    15781 attctataca aaagttgttt aattcgcttc ggttatgaac ttagtgaaac cctttgcaat
    15841 atatggtaca gaatatagaa attattgaca ttacagaaaa tgatgaagat ttaatacaga
    15901 ttgtcactat ttgtagatac cttttacaag gaaaccgagc caataaagct aagaagatga
    15961 ttaaggagtt gatggaagac gatacaagtt aagaaagtga aactacgccc aaataaagag
    16021 caaaaagagt aattggacgt ggaaaatgca agaaatgaac catcgattcg gtggacagtt
    16081 tattagtaat gacggtttga gaacatccat gacgagaatg aaggaacatc ctaaatgcgt
    16141 ttagttgaac caagtgccaa ataatatggc caaataagcg atcagttatc gatgaatccc
    16201 aagtattcta aactatatat tcattttgat gctaagcatt gatatatttt tttgggattg
    16261 atgaaggtta accaatacag ggactcactg atgaaattct agagtctgac cttggtatca
    16321 acgatttagt gattgtttct aatggaaaag tataaaagta tataagtaca ttaacaaaat
    16381 aactattagg gttacgctcc agcttgtgca aggcactggc aggaggaatc gtgaaatcat
    16441 aattatggtt aagccacacg gacaactaaa agtcatacaa agctatcgaa tcaatcaaca
    16501 gaagacaatc ggcaatcatt agctttgttt acagcttaca ttgcaacgct tgagttgaac
    16561 cgaaaatacg agggaggtaa cgcagccatt ttgagttggg gaatcttttt agaaaattat
    16621 cgggtacctt gttgtttagt caaaattata agggggagta atagtgagga acaaatttca
    16681 taaactgtta agtatgtttc tagttatgct tattgtgatc aatcaatttg ggaacataac
    16741 ggttctagct gagtctttag cagaacagcc agagcgttcg ctgacgatcg aagttgatca
    16801 agaggaggct gctttgggtg agcagttcca gttgcgagtc agaggaacac cagacctgct
    16861 aaatgcattg gtcattgata agagtgagga tttgattgaa ggggaagtga agcaactcaa
    16921 tgattacgaa cgtctgatta cttattcttc ccagcattca ggtaaatacg aattgaccgc
    16981 aagaagcggt gatattcaat caaatacggt ctcaatagaa atcagtgtac cagaagaaag
    17041 cacaagtgag tcttttaatg taccagaaga aagcacaagt gaatctttta atataccaga
    17101 agaaagctta agtgaatctt ttaatgaagc cagatcatct gagccatcta taacgcagga
    17161 atctcagatt gaaagacaag aggagctctt gaattatgac atcggcatct ctgctgagcg
    17221 ccccactttg ccgagcaggc atacacaaac gttaaaggga tacattcaag gaggcgctgg
    17281 cgatattctt gaagaatatg gggagatggt cgtacgagtt cctagagcat cgattggctt
    17341 aattgagttc attagagtgg aggtcgctga tgattcacct ttaaagccac tggcatttta
    17401 ccaagaaggc acagacgttg tttttccatt tacctttgat tatgaaggat taaatgaagc
    17461 cgctcagtcg tatacattca gtatcacgtt tgaagttgct ttctttcaat caggcggtac
    17521 tgagccaaat aatgactttc aagtgagtct tacttatgct gagcgagcag caaacacgga
    17581 attttcaatt acgcctattc cttcatcaag acccattttt tcgaaattat actatggctc
    17641 tagagaaatc gacagccaag gaaatcttgg gaatctgctg attgacgggc aagacgtgac
    17701 caacagtaat aaatttattt tgcaggtaaa ttatgaagga tcacgaaaag acggtgtagt
    17761 ggttaccgat gtactaccgc aaggaacaac tttggcaccg aatccgacta catggagttc
    17821 cactggaaat agagaaccta tttcaggtgg ggttcggatc gcagaagtaa cgaatccagg
    17881 tgaagcaaac gttagaactc attatgtttc cgaggaattt gtggacaaag tatcttttaa
    17941 taaggaaacg aatacacttg ttgtggactt tggtaatcta gcagagggtc aatcgtattt
    18001 tattgaatat gctcttgaag tgaccgatgt cgacttgttc aatgaaggaa gacaagagga
    18061 tgggttttct cggaatcgag cgagtatgac ctacaataat caggtaatga gccgagacta
    18121 ccgcttgaga atccctgaaa gtaatcttcc ggcaatctct ttaagaaaaa gtgtggacaa
    18181 aaatgttatc aatctcaatg aaaatgaact aacgtatact ctgcatgtct cgatgttttc
    18241 tggaacaaga aatacgcttg ttattgaaga tgttttggaa gaaggcatgg aatttgtgtc
    18301 atttctatca gatattccct ctttcattga tgccgaagtc aataatggaa tagttcgttt
    18361 cagtgcgaat aatacgattg gtggtccgac aagttttacc ctctctttta aggtcaatgt
    18421 agaggcgtat gcggtcggcg atcaaatttt aaactttgca acgcttgtga atagtgacaa
    18481 tcgattgaat acgagtactg taagaacaag gaaaatcgat gggcgtatta aagtaattaa
    18541 aactgatgag aatggccaaa ggctggcagg agcaaaattt gaaattttat caagcgattc
    18601 acaagtgata caagaaggta taacggcaga aaacggagaa tttttaagtg aaccgcttcc
    18661 tgtaggtagc tatcaagtcc gagaaacaga agcaccggaa gggtatcttt tagatgcgac
    18721 ggtacatgat gtttttataa atgaatactc catagaagaa gtgacgttaa ccattgaaaa
    18781 catccttgat acgggtggtg ttgaactaat taaacatgct gaaggcagtg gtgaagtcct
    18841 tcaaggagct gtttttgagt tacagaaccg agaaggagaa acgctacaaa ccggtttaac
    18901 tacaggagaa gatggaaaac tggcaattga cggtttagca cc
//
LOCUS       DACGLW010000057        18885 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.059, whole genome shotgun sequence.
ACCESSION   DACGLW010000057 DACGLW010000000
VERSION     DACGLW010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18885
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.059"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            <1..435
                     /locus_tag="IXT53_001946"
     CDS             <1..435
                     /locus_tag="IXT53_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HAQ4905749.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(759..842)
                     /locus_tag="IXT53_001947"
     tRNA            complement(759..842)
                     /locus_tag="IXT53_001947"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(806..808),aa:Leu,seq:cag)
     gene            1370..1870
                     /locus_tag="IXT53_001948"
     CDS             1370..1870
                     /locus_tag="IXT53_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ4905750.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(2060..2404)
                     /locus_tag="IXT53_001949"
     CDS             complement(2060..2404)
                     /locus_tag="IXT53_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HAQ4905751.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(2558..3478)
                     /locus_tag="IXT53_001950"
     CDS             complement(2558..3478)
                     /locus_tag="IXT53_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ4905752.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            3665..4156
                     /locus_tag="IXT53_001951"
     CDS             3665..4156
                     /locus_tag="IXT53_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HAQ4905753.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(4180..6555)
                     /locus_tag="IXT53_001952"
     CDS             complement(4180..6555)
                     /locus_tag="IXT53_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HAQ4905754.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            6881..7507
                     /locus_tag="IXT53_001953"
     CDS             6881..7507
                     /locus_tag="IXT53_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ4905755.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            7530..8234
                     /locus_tag="IXT53_001954"
     CDS             7530..8234
                     /locus_tag="IXT53_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ4905756.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            8426..9259
                     /locus_tag="IXT53_001955"
     CDS             8426..9259
                     /locus_tag="IXT53_001955"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HAQ4905757.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            9456..11384
                     /locus_tag="IXT53_001956"
     CDS             9456..11384
                     /locus_tag="IXT53_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HAQ4905758.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            11522..11977
                     /locus_tag="IXT53_001957"
     CDS             11522..11977
                     /locus_tag="IXT53_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HAQ4905759.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            12447..12716
                     /locus_tag="IXT53_001958"
     CDS             12447..12716
                     /locus_tag="IXT53_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905760.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            13116..13370
                     /locus_tag="IXT53_001959"
     CDS             13116..13370
                     /locus_tag="IXT53_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HAQ4905761.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(13459..14505)
                     /gene="holA"
                     /locus_tag="IXT53_001960"
     CDS             complement(13459..14505)
                     /gene="holA"
                     /locus_tag="IXT53_001960"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HAQ4905762.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(14543..16816)
                     /locus_tag="IXT53_001961"
     CDS             complement(14543..16816)
                     /locus_tag="IXT53_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HAQ4905763.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            complement(16809..17348)
                     /locus_tag="IXT53_001962"
     CDS             complement(16809..17348)
                     /locus_tag="IXT53_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HAQ4905764.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            complement(17484..17702)
                     /locus_tag="IXT53_001963"
     CDS             complement(17484..17702)
                     /locus_tag="IXT53_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HAQ4905765.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            complement(17699..17944)
                     /locus_tag="IXT53_001964"
     CDS             complement(17699..17944)
                     /locus_tag="IXT53_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ4905766.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            complement(18060..18788)
                     /locus_tag="IXT53_001965"
     CDS             complement(18060..18788)
                     /locus_tag="IXT53_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComEA"
                     /protein_id="HAQ4905767.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
BASE COUNT         5799 a         3654 c         3317 g         6115 t
ORIGIN      
        1 tactcaggac aacaattaac aatcccaaca gaaggctctg cacaagcagc tgctgaacct
       61 caagcagccg ttcaagaagc accagtccaa gctgaacctg ttcaagctga acaacctgtt
      121 gtacaagaaa cggttcaaac ggaaacacaa gcagcgcctg ttgctgaaac acaacctgct
      181 cctgctgtaa cagaaactgc tgctactcca gcaagtacaa gctctgcaaa agaatggatc
      241 gcacaaaaag aatcaagcgg ttcttacaca gcaacgaacg gacgttacat cggccgttac
      301 caattagatt cttcttactt aaatggtgac tactctgctg caaaccaaga aagagtagca
      361 gaacaatatg ttacttcacg ttatggttct tgggaagcgg cgaaagcatt ctgggaagca
      421 aacggttggt actaagattc attatttgaa cgtttaaaca cattcaaaat atagtaagag
      481 gtcatgcgat ttattctttc gcatgacctc tttttgagtt taagtttagt ctttattcct
      541 taactatcgt ttgccagtat ttcttgtatc agaatctata aatctactat ttgaatcaat
      601 ctattaggtt ttaaactgtc ccaccttcac acaaattttt tttgatttat ttgaatctgg
      661 ttccgttgtt taaaattttt cctatattct tttagataat atgttatttt ctttctcatc
      721 gctccaggtg taccttctct ttataaaaaa acaaattatg cggctaagaa gactcgaact
      781 tccacgaggt caccctcact agcgcctgaa gctagcgcgt ctgccaattc cgccatagcc
      841 gcatcacatc ttttagttta ggattacatg tgttttttgt caagaacata tttttgttct
      901 ttttcatttc tgtacgccct cccttatgcc atctacacaa ataattcatc cgagcaactc
      961 catcatctat tgagggttgg tgaaaaaatc tgttttatcc atatgatcga tttcaatgtt
     1021 aaacgaacga ctctgggatt agtaactatt ttattttttt cttcatttct tttttcctct
     1081 ttttctcgat tcagctatct tctttcattt ggatattgat tatttcttct gttgattact
     1141 atccttctgg aattctagac gctgaagtaa ttcacgtttt ctttcttcta gagatcataa
     1201 gtaacgaatg aacgaacttc aaataatttt tttattgtaa ttcatatttg tattgttcga
     1261 tgattcttaa tacgatatga cacaataaat aaaaataggc atggctattt acaacaatag
     1321 tgctatagta gaaaacaaat gaataaggtt acttaaagag gtgaatacga tgaaactgat
     1381 caaactatgg gaagcagatt taaagaaagc ttatcagcta caaactagct ttggaccaaa
     1441 tgagagtgga tttatcaaca atgcttatgg ctatacattt gctgaatttt tagattatgt
     1501 aaaaaaatgc gaaaaaacgt ctttaggaat cgatcttcct gagagttacg ttcccgcaac
     1561 cgtctttatt ttagaaagtg atgaacatga gtatgtagga atttttaatt tgcgccataa
     1621 actgaacgaa acattaagaa acggcgctgg ccatattggt tacggcatct cccctaatta
     1681 tcgtaaaaaa ggatatgcca cacaaggatt agcattagta ctacaagagg caaaaaaaat
     1741 cggcattgat gaggcacttt tgtctgtaaa taaaaaaaat cttggcagtc ttaaagcaca
     1801 acaaaaaaat ggggctgcga tttatcgtga agatgagaaa gaatactata cgaaaatcaa
     1861 actgatgtaa aaaagaagga ttccaaaagg aaagggattt gaatgtcctt tatcaataga
     1921 acacatttat ttaattaact tataatatag cagacaaaat tttataaaca aagaaacctt
     1981 ttatctcttt tcttctagaa aggataaaag gcttttttgt ttttcctatt tgcttctcat
     2041 agctagtcag ccaagatttc tatcgtatcc ccaacgcaat ccgtgcataa cggctcatct
     2101 tatctgtcgt ccatgctgga taccagacta gtttgacttc tgctttcgtt acttctggca
     2161 cttcttttaa tgcgtcatgg atagattctg ttaaaatatc tgctaatgga cagcccatag
     2221 ttgttagagt catcttaatg actgtttctc cattttcagg attgaattct atatcataaa
     2281 tcaagcctaa attgacgatg tcgattccta attcaggatc aatcaccatt tccaaggcag
     2341 caagaatacg ttctttgatt agttctattt cttgtccaga ccgttcttgg tttgcttcgc
     2401 tcatcgtacg acactcctta acacgatttt tatttatcat actctttttt tagctgcttg
     2461 taaatgagaa tttagaagta agattctcgt ttaaatttat aaaaatgttc ttattagatg
     2521 taatagatta aatttattct aaaataaacc tgattcctca ttttttttgt gaacgatttt
     2581 ttatttctgg caatacgcca gttgttcgat acattttttg ttcgttttca tttgcaaacc
     2641 ggccaaatac tgaatgagtt ggtaaaatat tgataaatgc atcgtcgtcg atttcatgaa
     2701 cgatctgatc aagatcatac atttcgtagc gggtgatgac catcatgatc atgttccctt
     2761 gtttttttga atagccaccg acagacggca gtaatgtcat tccacgtacc atccgttttg
     2821 ctagttcttc tgttacctct tctggcttga cggtaacgat cattgctgtt actttttgat
     2881 gactcgtgtg gatggcatca attacttgag tcattgcata aatggaaata atcgtataca
     2941 aggcactttc ccagttaaag aagaagcctg cgatgactac aatgatacca tttagaatca
     3001 gcatgtagtt tccgactgtt tttccagttg tttgtgaaag tacaagagag atgatatcca
     3061 tcccaccagt tgtaaagccc atttttaaag ataatcctac gccaagacct aataaaacac
     3121 cgcctactag ggcattcatc aaaatattat cagtgatctg tccgactgga agcagcatcg
     3181 tcatcaccga tacacataca acattcacaa aactaaggat cgttgctcgt tttcctactt
     3241 tgagaaaccc taatacaaac acagggatat tcaaaagaaa aataaatgtt cctgtgtcta
     3301 tcttgatatg aaaccattca tataaaagcg aggaaatgat ttgagcgatc ccattcatcc
     3361 ctgcagaaaa gacattggct ggaatcaaaa aataatttaa agcgaccgca gcggtcaatc
     3421 cggttatcaa aataaccact attttcttta atctttcatt tttatgatac gcagtcataa
     3481 ttttactcac aagcacctct cccctacttt ggtattccaa atgtaagaat aacagtttat
     3541 ctattaaaat aaaatacttc gaactcaaat gagaagaatt ttagcaaaca ggtaagaatc
     3601 tgccctaatc ctatgataag atagatgtat ggtaaatatt tatatgtata tcaggagggt
     3661 tcctatggca aaagatgcta gcttcgacat tgtatcagaa gtcaatacag aagaagtaaa
     3721 aaatgcgatt cagcaaacaa caaaagaatt gtccaatcgc tttgacttca aggattctac
     3781 agtagaaatc aaattagaga acagcaatct agttgtattg agcgatgatg atttcaaaat
     3841 cgaacagatc aaagatgtat tatttggtaa gctgatcaaa agaaatgtgc caatcaaaaa
     3901 tatccatttt tccgaatcag aacacgctct aggtggcaaa gctcgacaaa ctgccgattt
     3961 ggtcaacgga atcgatcgtg aaaacgcgaa gaaaatcact acagctatca aaaatgaaaa
     4021 actgaaagta aaagcacaaa tccaagaaga tcagattaga gtgacaggta agaaccgtga
     4081 tgatctacaa gctgtgattg ctttattacg taaacttgac ttaccaatcg agttgcaatt
     4141 cactaactat cgttaatcaa aaaacgaggc tgacgtctgt cattcctcgt ttttttgatt
     4201 tactgcttct tttgataacc aaaaacgctg tattccttct tccaattccc caacacttcg
     4261 tacttctctt gctgtttgcc aatgttgtgg gaataaacgt cgataagaaa aactagaaaa
     4321 tcgttgatct gccaatacaa cgattccttg atccgccatg ccacgaatca ctcgaccacc
     4381 agcttgcaag actttgttca ttccaggaag ctgataagcg taagcaaatc caaggtgttc
     4441 cttctcatca taatatgatt tgatcagttc ctgctcatga ttcatctgcg gcaaacctac
     4501 acctacgatc atactgccga tcaatcggtc ccctctcaaa tcgattcctt ctgaaaaaat
     4561 cccgccaagt acgcaaaatc caatcaatgt ttcagcggga tttgtttgaa attttgttaa
     4621 aaatcgttcg cgttcttcct cattcatgtc cgtttcttgg attagcacat tgctgtcggg
     4681 atattgctgt ctgaatgctt ccaccgcaag atctaaatat tgataagaag gaaagaaaac
     4741 gaaataattc ccgacctttc catcagcaaa agcatgaatc gtctctacta attttgccag
     4801 actgtgttcc ctttttcgat aagtcgtctc taggtaattt gcgatgaaaa cttgctggtt
     4861 tccttctggg aaaggactcg gcagtttgta agccagactt ttttgtcccc ccagtgtttc
     4921 ctgataataa gagagtggtg aaaaactagc agaaaaaaga atactgcttt ttcctttatc
     4981 caacgactgc tcaagaaaca agcttggatc aatacaaaac tcttttacta tcagatcgcg
     5041 atacgttttt tcgactgttg tttcgtagtg atcatcataa aattcactga ccttcaaaaa
     5101 atgaagcaaa tcaaaataat aaggaagcaa ttgttcttgt gatgggtgct caggaaattc
     5161 tgccagccat tctttaattt tttcgcttaa ttgataacct gcctttacta aagattcagc
     5221 cggtgctttt tgatgatgat aatcccaatg atcttctttt gctatttctc ggatcgaatc
     5281 aaattctttt aatagtttgt tgaaacggcg atgaagtttt ttgaattcct ttggtatagc
     5341 ttccttactt cgtttcgttt tttcataaga tagttctgcc gaatacatct ctcgggaacg
     5401 attcaccaaa ttatgtgctt cgtcaatcaa aaacagatag tcctcgtttt tttcatcttc
     5461 aaaaaaacgt cttaaataaa cagttggatc aaataaataa ttgtaatccc caatgattac
     5521 gtcacaccag atactgacat ctaatgatag ttcgaaagga caaagtgtat gctttcttgc
     5581 ataagtttcg atgacttcac gagtgatctg attctcatga ttcaaaagat cccataaacc
     5641 ttcattaatc cgattgtaat aaccattggc atatgggcac tgatctggat tacaggtcgt
     5701 ctcatctaaa aagcagatct tgtctttcgc cgtcaaagtg acacttttta cttcagctcc
     5761 tgtatctttt aatgctgtca tagcatcttc agctacttgt ctagttattg ttttagccgt
     5821 tagataaaag atcttttctc cctcttcttc ccctacagct ttcaaagcgg ggaataacgt
     5881 agagattgtt tttcctgttc cggtaggtgc ttctacaaac aatttctgtt tcgtacggat
     5941 cgttttataa gcagctaccg ctagttcacg ctgacctttc ctatactcat catgggggaa
     6001 tttcaaagcc atcaaagagg cattgcgtac agtgcgccag ttttcttgaa aaacaagcca
     6061 ttctttataa tcttcagtca gctttttaaa aaaagtttct aactgctctc ttgtctgatg
     6121 ttcaaccttt ctcgtgatca cttcttctgc cgtctggaag taagtcaatt gcagatttat
     6181 ttcttgtaaa tcatgttctt gactatagat ataggcatac acccttgctt gatgaaagaa
     6241 aagttccact tgttctggtt ctaaatcttc aaatcgtggt tcggaggttt tgatttcatc
     6301 tatataataa ataccgtctc ttttgaaaat accgtctgca cggccttcaa cgatcaggta
     6361 atattcatct aattgaactg ttgtttttaa aaagacttct ttttcatagt tttctcccgc
     6421 atctttttgg agcttgcggt ggattctcgc tccttctaat gcagtatggt tacttttttt
     6481 acgattgtcg atacttccac gtcgcaaaat aaattctact aagtgccgca cagatatttt
     6541 tgactgtttg atcatttcca ccacctcttt ctctctaaga ttacctgaaa aaacctagtt
     6601 tagcaaaaag agctctcttt ccaaacataa aaaaacaccc tgctgattag gcagggatat
     6661 aggatataga atttggattt ttggatgaat ctaatgtaac aaggacatat taaaatgcta
     6721 tgaaaactta ctttccttct ctttagaata tccaaatact tttcatatag tctgataatt
     6781 cagataagta aagagaaata agtacctgtt tttgtttcct agcaatgaat gcgttatact
     6841 aatggatgaa agaagttttg gctatcaagg aggaatgcac atgcatatcg aaagattaaa
     6901 aacaggaatg attgaagaaa actgttattt ggtctacaac gaagaagcac ttttgatcat
     6961 tgaccctgga gaagatgcgg aacgaatcaa acaagaaatc aaaaaaacaa accaacagcc
     7021 ggtagcgatt ttgcttaccc atactcacta cgatcatatc ggtgcagtgg aacctttacg
     7081 cgcgtattat caaatcccag tttatgtcag cccactagag caaaaatggc taggtgatcc
     7141 gattttgaat ctttctggac tcggtagaca tgatgatatt gctgatgtta ttgtctcgcc
     7201 tgcagaatat gaatttgaaa tgaaaactta tactttagga aacatgtctt ttcgcgtcgt
     7261 ccctacccca gggcattcta taggaagcgt cagctttatt tttgatgatt ttgtcgtttc
     7321 tggcgacgcc ctgtttagag gaagtatcgg tagaaccgat ctgtatacag ggaatttaga
     7381 acaattactt catagtattc ggacccagct gtttgtatta cctgatgaat ttgcagtcta
     7441 tccaggacat ggcgatgcaa cgacgattga acaggaaaaa aggacaaatc cttttttcaa
     7501 tcgttaaaac gacttaggag gagaataaaa tgacagaaac tacactttat atcgtacgac
     7561 acggaaaaac gatgttcaat acaattgaac gagtacaagg ctggtgtgat acacccctca
     7621 caaagcaagg acaagaaggc attcattatt taggtcgcgg gttaaaaaat gtcgactttt
     7681 catttgctta ttctagtgac agtggtcgtg caatcgaaac tgcccgtatt attctaggag
     7741 aacattctaa gggaaaagaa attccttatt ttattgaaaa aaggattaga gaatggtgtt
     7801 tcgggtctct tgaaggtgga tatgacatgg aaatgtgggg tgtgatccct cgtgtattga
     7861 attttaaaac atatgatgaa atgtttacca cagaagtttc ttttgaacaa attgctaatg
     7921 ctgtatacaa cttagatacc gctgggtggg cggaaactta tgaaacattg cgagaccgtg
     7981 tatggacagg atttgaagat atcgcccatc actgcgaaaa aaatggtggt ggaaatgttt
     8041 tagtcgtatc tcatggtttg acgatcgcct ttttgcttaa tctgatcgat cctaaacagc
     8101 ctgttcgtgc tgggctttta aatggcagcg taacaaaggt cacttatgca aacggtcgat
     8161 tttctatcca cggcatcaat gatacgtctt atatcgataa cgggaaaaca catagtcgcg
     8221 actcgtttct ataatgggga tattttaatt tttaccagag aatcataaaa ggcaaagtct
     8281 gaacgagggc tttgtcttct tttttctctt cgtgtactta tctgctttat ttctcaagtt
     8341 tttcttaata ttaaaaaaca gattgggttt ctcaaaaaat aacggtatac tataagatgt
     8401 atcttaattg ggaaggaagg aatttatgtt ttttgcaaat attatcaaag cagtgattct
     8461 cggtattatt gaaggaataa cggaatggct gcccatcagt agtactggtc atttgatcct
     8521 agcagatgaa ttcatcaaac tggatgccag ttcacaattt atgtcgatgt tcaatgtcgt
     8581 tatccaatta ggggcgatct tggctgtcgt cgttctttat tttcacaagt taaacccatt
     8641 ttctccaagt aaatcaacgg tagaaaaaaa agatacgtgg acactttggt ttaaagtgat
     8701 cgttgcttgc ataccagcca tcatcattgg tttgcctttg gatgattggt tagaagcaaa
     8761 tttccacaag ttcttaccag ttgccattgt tttgatcgtt tacggtattg cctttattat
     8821 tgtcgaaaaa cgcaacaaaa acaaaacacc tcgttggact tctctgaatg actttacgta
     8881 ccaagcagct cttcttgttg ggttattcca agtcttggca cttgttcccg gaacatcccg
     8941 ttctggcgca acgatcctag gatctatctt gattggtgct tcgcgttttg tcgctacaga
     9001 gttttcattt ttcttaggga ttcctgtcat gtttggcgca agcggcttga agatcgtcaa
     9061 atatttagct gaagggaatt cattcaaaat ggaagaaact gttgttcttc ttgtaggaac
     9121 attcgtatct tttgtggttt ccgtcttagc tatcaaattc ttactaagct atttgaagaa
     9181 aaacgacttt accgcttttg gctggtatcg tattatcctt ggtatcattt tgattggtta
     9241 ttggtttatt gcaatgtaga aaaaaccaga acacttctct tatttgagaa atgttctggt
     9301 tttttatttt ctttaataat aatcattata aataataaaa acaacatcaa gcttaatcac
     9361 caaatgataa ccattatcgt tctcatttag ctgttattat tatcaattac atttttatta
     9421 tgctatcctt gttttgacaa ggaggtctaa taacgatgac taatcagaaa aaagcttttg
     9481 cagcaacaat cggctgcctt ttatttatga tcttcggttt tatcttcgac cgattctctc
     9541 ttaccatcac cccacttttt tatatcagtg ccattatcat cggtggatat aaacaaacat
     9601 cggaaggctt gaaagaattg tggaatgatc ggacactgaa tgtggattta ttgatggctt
     9661 tagctgctat tggtgcctgt ttgatcggta attactttga aggagcaatg ttgacattca
     9721 tcttttgttt aagcggtgca ttagaggaat acacaactaa taaaagtcaa aaagaaatta
     9781 ctgctttgat gaatcttcaa ccacaaaaag cccaattatt aaaagaaaat ggacaaatgc
     9841 aagaagtaga tgtttctcaa ctaaaggtgg ataacttggt atttgtcgct aaaggagcag
     9901 ctgttccgat agatggcttt ttagaaagca cacaggcaac gatcgatgag tctgcattaa
     9961 gcggtgaatc tgtgcctgta gaaaaatatt taggcgatga gatgtatgca ggaacgctga
    10021 atcaaggaaa tcccatcacg attcgagtaa caaaaacgag ttccgaaact gtctttgcta
    10081 agattatcca attagtagaa gaagcgcaga atacgccaac tcaaacagct tcatttatcg
    10141 aacgaatcga aaataattat gtaaaattga ttttgcttgc tgttcctcta atgatccttc
    10201 tcccccactt tttcctaggt tggagttggg atgaaagctt ttatcgagga atggtattat
    10261 tagttgtggc ttctccttgt gcgcttgtcg catctgctac accagcttct ctagctgctc
    10321 tttcaaattc tgcaaaaaat gggattttaa tcaaaggcgg tatccattta gaaaagcttt
    10381 ctgaattaaa agcgattgcc tttgataaaa caggaacgct cactaaagga aaaccagttg
    10441 taactgacag ctttttcttt gatgaaaagg atttggcaca acaacttctt gttgctatgg
    10501 aacaaaaaac gacgcatcca cttgctcatg cgattttgca atatttcgat tgcacaatac
    10561 ctgaagaaat ccaatactta ccaatagaag aaattactgg ttttggatta caaactacct
    10621 atttaggtgc gcaatggaaa gttggcaagc acgcatatga tcctgagacg atgataatca
    10681 gcaaagagat tgcggaaatg atcgaacgtt tggaaaatca aggcaaaacc gtgatttatc
    10741 tttctaaaga tcagcagtta attgctgttt taggtctgct agatattcca aaagcaaata
    10801 cccaacaagt aatttcctac tttaaatccc aaaacatcca tacaagtatg ataaccggcg
    10861 accattctgg cacagcaaag gccattgctg aacaagtagg gattgaacat tactatgcaa
    10921 gctgtactcc ggaagaaaaa acacaactag tcaaaaaaga gaatgaactt taccaagtaa
    10981 atgcgatggt tggtgatgga gtcaatgatg cgcctgcttt agcagccgct tctttaggtg
    11041 tcgctatggg gcaagggaca gatgccgcta tggatatcgc agacatcgtc ttaatgaaaa
    11101 gcgacttaaa taaatttgtc tacagtcatc ggctatcttt aaaaatgcgg cggatcatca
    11161 aacaaaatat tattttttcg attcttgtta ttctcttgtt gattgcttct aacttcctgc
    11221 aatttttaaa tctacctctt ggagttgtag gacacgaagg aagcacaata ttggttattc
    11281 tgaatggctt gcgtttacta agacctttac ctgatctttc tgattcttca aaaaaatgta
    11341 agtcttgtcc actatacaaa gcgcggctgt cttctgtcaa ataagcaaag tgttttttta
    11401 tgattttgtt tattttatga aaaaaacagc ttatgaatag gtcttgaagg atgaattcct
    11461 acttttgtaa ctttctaaaa acatgttatg attgtttcga agaaagtgag gaatatattt
    11521 catggaaagt tggctttttt taggaattat tttacttgta gcaattttag ctaaaaatca
    11581 aagtctgatg atagcggcag cttttgtttt gattatgaag ctcataccag cctcccgttc
    11641 ctttttccct tggcttcaag gcaaaggaat caatattggg gtgacgatca tcacagcggc
    11701 tattctaatc cctatcgcaa caggagaaat cggtctacgt gatctattag aagcctttaa
    11761 atcccctatt ggctgggtag ctgttttttg cggtgggcta gtggctgtct tatcttccaa
    11821 gggtgtagga ttgattgctt ctgatccaca aatcactgtt gcacttgttt tcggcacgat
    11881 tttaggtgtt gtcttcttaa gaggaatcgc cgccggaccg gtcattgctt ctggtatcac
    11941 ttactgtatc ttacaagtat ttgctttagg aaaatagaac aaattttgtc tattttagga
    12001 gattcttaac acccccaatt tactgatccg tgatatactg acaacgaggt gatacaatgt
    12061 tcgaaaaatt aaagtatttc ataggaacaa tcggctggtt tcttctagcg gtacttttga
    12121 ttctgggctt agcttatcta ttatttcctg atttgataca aaatgtgttt gatactgctt
    12181 tttgattgac ctattaaaac gatatagggg atgctcctgc gatgcagggg ctttttttaa
    12241 tcaatgtttt gtaataattt aggccgtgcc aatgagactt cctatagaat tcgatgctct
    12301 tttttctttt tggctcttct gctctttttt gttatactag attacagtgg acaaaagaaa
    12361 gaatgatcgc atccacttca acgaatatca aaatggaaga attagtgcct gttttcaggt
    12421 tactttgaaa agaaaggaag acgcttgtgg aaacttttga aggtctttta attgatacgt
    12481 acgttttaca agaacagcca atgatgctga ctgggatcaa cttgcagctt gaggaccgtg
    12541 tgatcaatgg cctgcttaaa gatcgctatt tggcaaaata tatcttacgt ttgcccctaa
    12601 ataaatatgt gctggaagta agtggcaaat ggaaccgtcg gaaccagttg gtcataaaaa
    12661 aaatgttgat aaaaaatatc gatgaataca ttaaaataat cggtacacca gaatagcctt
    12721 tactgatcaa aggatactgg ttataaaata actgcagttt cttaatagaa aaacaaagaa
    12781 caacagggaa tcgtatccct tttcaaaaaa aactttgcaa acggttctct ttgttatata
    12841 ataacttagt accaaacatt cccattcaaa ctttgtttaa atagataaat aaaaatctag
    12901 tttggtttga cgctattgcc tcccacaata gcgtcttttt gatttttttc agcttgtact
    12961 atccgttttt actgaaaaaa aatcctaata aaagactgtt aaggaaaaaa acttgtcatg
    13021 ttcatattgc aaagcacgct gcctttaggt ataataatga atgttgaatt atgcggtaac
    13081 acgcgtaaga acttaagtta tcggaggtga aactaatgcc aaacattgaa tctgcaatca
    13141 aacgtgttcg tacaagcgaa aacgctaatg tgaaaaattc atctcaaaca agcgctatgc
    13201 gtactgcaat caaaaaattt gaagatgctg tagcttcagg tgctgacaac gtagatgcgt
    13261 tatataaaga agctgttaaa gctattgata tggctgaatc aaaaggtcta atccacaaaa
    13321 acaaagctaa ccgcgacaaa tctcgtttaa gcaaaaaaat cgctaaataa aagaatcggc
    13381 ttcggccgat ttttttattt gtcaaaaaaa ggacaaaaac aaacgacctt cccgcatgct
    13441 ttgccctcgt ctttcttctt atcgatttct taacaactgc cctgtgcgca aaacgaatag
    13501 ttgaaaactt aattcttttt cgatttgtcc cgttttgatt tgataatcca tctcaactaa
    13561 ttgatcaaac aactgtacca gcaaatgttc atcgaatttt tttacttcct gcatggcaag
    13621 ttttacacga tatggatgga ttttcaatgt ttcagcaata tttgcctgct gataaccaat
    13681 ctttacaaga aattttgtct gtaaaaataa acgtaattga gaaagcaaaa tcgcattaat
    13741 tttgattgtt tcttcccctt gcataactaa atcttcgtat aaacgcaaag cttgttccgt
    13801 cttccctttt aaaatatatt gggtcatgtc aaaaatatta tgttctaacg ttttaggaac
    13861 taattcttct acttctctta ccgtgatttg ctgttgattt tgtccataaa ggaccaattt
    13921 ctgtaactct ctcatcattt tggataaatc taagtcggtc aacctcaaga ataaatcaat
    13981 tgcacttcgt tccattttga ttgaggaatt ctctaaggta ttcatcagat attgacgtac
    14041 ttctttttca ttcaactgtt ggacattgat gataattgct gtttttttca attgtttcgt
    14101 tacttttttc cgttcatcca acttttcata tggcgcaaag aatactaata cagtcgattc
    14161 taatggttct ttcaaataag ccgtcaataa atcagtattt tgttcgattc cattgtttac
    14221 tttttcacct gttaaaaaat aaggattctc tacgaatact aaccgttgat ctccgaaaaa
    14281 cggcaacgtc tctgcttcag ctactacctc atctaaactt gcattttcca tgtcaaaact
    14341 taaaaaattt aagtcatctt cgccctctaa ttggattttt ttgacaattt ctgtccgaac
    14401 ttgatcttgg agaaactttt ctgttcctaa aatcaagtaa caagatgcaa gtttttcttt
    14461 acgtattttt tgtaactctg cttgtatact caaattactc accactttca aaaattatcc
    14521 taccatgagc agatgagaat tactagtcta tcattgtttc cactagccca tgaggatgaa
    14581 aaactggatg ccagcgatag gtgatcattc cttgttgatc cgttcgccat gtttttatct
    14641 gattttcttc tagtgtctcc aatacttcgc tgtgcgggtg accataacga ttatttacgc
    14701 cgcaggatat aatcccatgt tttgcttcaa tatgggaaag aaaatctgtg ttacttgacg
    14761 tacggctacc gtgatgacct aatttgacta catcggcttt cagattagga taaatcaaaa
    14821 gcaattgttt ttccattgct tgattcaaat ctcctaaaaa taaaaagcgc ctattgttat
    14881 attccatcca tagaccaatc gaatcttcat ttcttccctc tcccttttca aaaggagtca
    14941 atacataaag atcataataa ccgctgatcc tgtccccatg tttgatttca atgatctcag
    15001 taccattttt ttgagcttca gataaatgcc tttgtaatgg ctcatttcgt ccagcgcccc
    15061 aaccgatata aatttttttg atagtaaaat gctgagataa cgtgggtaat tctcccacat
    15121 gatccgtatc gtcgtgtgtc aagatgagtt tatcgattac acttatcccc tgacctttta
    15181 gatatggaac gatatttctt tcagcaggag gacgtatcat accttgcgcc cacttttctc
    15241 tttcaaagtg taactgacct ccagtatcga tcaaaatcgt ttcttttcga aaaggacttt
    15301 gcaaaaagat actgtctcct tgaccgacat cgataaaagc gatcgttgaa gaaaagcgta
    15361 aaaatggcaa agtcaaaaaa ataaaagaaa aaagaccaac ataaaacaag tgtttcacag
    15421 gttgttcttt caacctatcc atacaaatca gtaatccgaa aatatacatc ccagatagca
    15481 caaggggcaa acgtcctata atgagagacg accctgaaga aaaatcgatt attcgctcaa
    15541 ataagattaa tccctcttct atcagcaaaa gtaaagtttc cggcagccag cgattgaaga
    15601 gtgtgagaaa taacacacca ggaagaaaaa gaaacttgaa taccggtgca atcagtaatg
    15661 taaaaagact acctaaaata ggccactcat aaaaatgcca tacaatcaac ggcatcgtga
    15721 tcatagtaaa caggacgtga gaggccgttt ttttcaacca atctccatca aaagaaatca
    15781 ggataaacaa aaaactcatg aaaaaactaa gctgtccacc cgcatgaaaa agaaatcgag
    15841 gatcaaatag taaaagaagc caaaagacaa ctgcaaataa gtccatggtg gaataatgct
    15901 tattcagcaa tttgactagc aatcgcaata aaaacagcaa actagcacgc aaaacagaaa
    15961 tagaacttcc tgcaatagaa acataacaga tagttagtag actcaacgga ataatactga
    16021 cgtgtagtgg ccatccgata cgtcgaaata aataatacag ccaccctaaa taaaattgga
    16081 tgtgtagacc cgatagactg aacagatgca gcaaaccact ctccctaaaa atttcttctg
    16141 gttctctgaa tcctgcatct ttatagcccc ataataaaga attaatatat acacttgttt
    16201 tttgcggaaa ttcagattgg acaaaggaga taaaagatcc tctttttctc tgaagccagt
    16261 ttcgccagtg attttgcatg gtgatctgtt caatcttttg aatttggaac cgtccagaaa
    16321 ttctatggtt cctgaagtaa gcttcttggt caaaattata aagattccgc ttttttgctg
    16381 gagagatgaa ctcgccttca gcagtcaatt ctattccctt tcctcggaag ttaagccatg
    16441 ttttcaattc tgcttctgtg tttacttgat aagaaagctg gagcttttgt ttattctctg
    16501 ataaccttcc ttccattgtc aaaaaattac catttgcacg aatcgtatct gtaaatacag
    16561 taatttgcgc agatgttgtt ttttcctcta aaaatgaata ctcttgtagc ctataagaaa
    16621 tggataccgt tatgctacac aaaatacaca agtacgcgat aaaacgtcct tcttttagcg
    16681 aaaaccataa aaaaattccg gcaagaagca aacgataacc ccaattgtca gtgtaataca
    16741 acaaacagat aatcatcgac gtaattgccg gaaaaatcaa ccgatttcga acatgccttt
    16801 ctaaaacgct agacatgttt tcctgttaat tcattttgaa gttttgaaaa atgctgtgag
    16861 tctaaagcaa cttggtgtac agatacacct aattgttcga tcaacgcttg ggcataagga
    16921 tcatttcgat agtcatggag ataattgatc cctttcacac cagcttgcaa caaagccttt
    16981 gtacaggcca aacatggaaa atgtgtgaca tagatttctg aaccttccgt cgggatacca
    17041 agttttgcac actgtaggag tgcattcatc tctgcatgga tcgttcgcaa gcagtgtcca
    17101 tccacgacgt aacacccttc atctatgcag tggacatctc cactgactgc tccattatag
    17161 ccgccagcaa tgatcctttt ttctcgaacg agtgtcgctc ctacttctaa acgtgtacac
    17221 gtactacgta aagataacag tactgcctga gccataaagt actggtccca aggaatgcgt
    17281 tcattatgtg acttatcagt aactttttct ttattcgtat cgcatgcttt tgaagatttt
    17341 tctaacatgt tttatattcc tccgtttttt caagttgatt aaaaagtact cattgcttaa
    17401 acaaaatatt ctttaaatat aacgaatcat cctgtctgac aaaagatcct acccttcact
    17461 gccgaaatga agtccgtcat ccattagccc attataaaaa ttcttcgaat ccatttgttc
    17521 aagaaattcc gtataaaaat cgatacttca caatcatggc gttttgctac ttctgtgaga
    17581 acagagatat attctttgat caacttgtta ttccgatatt tttgttttgt ctcatcgact
    17641 gctggtggcg tgataaagaa agcatgatca gaactatagt cttttttcag ctgaactatc
    17701 atccactgca gattttttgg tacgtttctt tagaaagttg tttatgctct gctagatcat
    17761 tcgtcccaat gagaataaat actttatctg ctgtatagct aagtacatct ttggcaaagc
    17821 gttctcgtag agcttgactg ttattgccgc tgacagccgt attcataata tgaatcccag
    17881 atattttatt ttggagatag acattcagca ttggtttttt taatgtttct ttgcgaacaa
    17941 tcaagctatt tcctatccaa aaatactacc tcctcgtcct gccaagctct tagaattgga
    18001 ttattcttag tatccattag cgtccattta ttgctcgtgc cagtcgattt tgaatcattc
    18061 tatttaattg taacgaagtt ttttagtttt tcaactgtct tttcaccgat accagaaatc
    18121 tcctgcaggt cttccacttt ctggaaggat ccgttttctt ctctaaatcg aatgatttct
    18181 tgcgctttga ctgatccgat cccaggaatc tgctgaagtt ctgatggagt ggctgtattg
    18241 atatttattt tgggaggctc ggaatctgct gtaggctgtt ttccttccga ggatgccaac
    18301 tgatcaagat gttctggcag ctcttcacct acagcaggga tatataggac ttgctgatct
    18361 tctatcttcg cagaaaagtt gattcgatcg ctatccgctt cttctaataa tccacctgca
    18421 cgctttagta catcatagac ccgttcttct gaagaaaagg aatagatacc aggctccttc
    18481 acacttcctt tgatatcggt gtacatgacc tttggacgag catcctcttc tacatcgttt
    18541 ccttcagctg atggatcttc tccactggaa ctaaacgaag aaagtatttc ttttgcagca
    18601 gtctcttctg tttctctata aaatagcaaa tttgataata aaaaaccact tgcgatacaa
    18661 aagataagaa ctatcataga catccaaatg atcaatttgg gtgaagctag tattttcttg
    18721 ctgatttttt ctatcattgt tcttttttca aagaatgacc agtaaaaatc attcattact
    18781 cctttcattt aaaattgaag aaacagtgag ctctggcaaa atcgtccctt caacatctaa
    18841 ttacgtcaat tttttctttt gtttttattt ttatcgtgaa gattt
//
LOCUS       DACGLW010000058        18576 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.060, whole genome shotgun sequence.
ACCESSION   DACGLW010000058 DACGLW010000000
VERSION     DACGLW010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18576
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.060"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            complement(<1..1253)
                     /locus_tag="IXT53_001966"
     rRNA            complement(<1..1253)
                     /locus_tag="IXT53_001966"
                     /product="16S ribosomal RNA"
     gene            1173..1316
                     /locus_tag="IXT53_001967"
     CDS             1173..1316
                     /locus_tag="IXT53_001967"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905768.1"
                     /translation="MEKEAFDLHVLGTPPAFVLSQDQTLIKSSNNLAIVKLNLFASIL
                     LAC"
     gene            complement(1939..3444)
                     /locus_tag="IXT53_001968"
     CDS             complement(1939..3444)
                     /locus_tag="IXT53_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ4905769.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(3441..4127)
                     /locus_tag="IXT53_001969"
     CDS             complement(3441..4127)
                     /locus_tag="IXT53_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ4905770.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(4320..5741)
                     /gene="gndA"
                     /locus_tag="IXT53_001970"
     CDS             complement(4320..5741)
                     /gene="gndA"
                     /locus_tag="IXT53_001970"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HAQ4905771.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
     gene            complement(5911..6090)
                     /gene="rpmF"
                     /locus_tag="IXT53_001971"
     CDS             complement(5911..6090)
                     /gene="rpmF"
                     /locus_tag="IXT53_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HAQ4905772.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(6148..6717)
                     /locus_tag="IXT53_001972"
     CDS             complement(6148..6717)
                     /locus_tag="IXT53_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HAQ4905773.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            6903..7805
                     /locus_tag="IXT53_001973"
     CDS             6903..7805
                     /locus_tag="IXT53_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ4905774.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(7840..9624)
                     /gene="pyk"
                     /locus_tag="IXT53_001974"
     CDS             complement(7840..9624)
                     /gene="pyk"
                     /locus_tag="IXT53_001974"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HAQ4905775.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(9698..10660)
                     /gene="pfkA"
                     /locus_tag="IXT53_001975"
     CDS             complement(9698..10660)
                     /gene="pfkA"
                     /locus_tag="IXT53_001975"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HAQ4905776.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(10828..14142)
                     /gene="dnaE"
                     /locus_tag="IXT53_001976"
     CDS             complement(10828..14142)
                     /gene="dnaE"
                     /locus_tag="IXT53_001976"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HAQ4905777.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KRQLIYLLGEDNVVFK"
     gene            14373..14558
                     /locus_tag="IXT53_001977"
     CDS             14373..14558
                     /locus_tag="IXT53_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HAQ4905778.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(14620..15816)
                     /locus_tag="IXT53_001978"
     CDS             complement(14620..15816)
                     /locus_tag="IXT53_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ4905779.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(15986..17422)
                     /locus_tag="IXT53_001979"
     CDS             complement(15986..17422)
                     /locus_tag="IXT53_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HAQ4905780.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            17715..18524
                     /locus_tag="IXT53_001980"
     CDS             17715..18524
                     /locus_tag="IXT53_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ4905781.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         5346 a         4001 c         3291 g         5938 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatgcaccgc gggtccatcc atcagcgaca
     1021 cccgaaagcg cctttcaaat caaaaccatg cggttttgat tgttatacgg tattagcacc
     1081 tgtttccaag tgttatcccc ttctgatggg caggttaccc acgtgttact cacccgttcg
     1141 ccactcctct ttttccggtg gagcaagctc cggtggaaaa agaagcgttc gacttgcatg
     1201 tattaggcac gccgccagcg ttcgtcctga gccaggatca aactctcata aaaagttcga
     1261 acaatcttgc gattgttaag ctcaatttgt ttgctagcat attgcttgct tgttaaaaat
     1321 gtttgttgtc ttgaattgaa tcaagacgcc ctacacattt ggtttgtctt actttgttca
     1381 gttttcaaag gtctaatctt tttgttttaa caactcttta atcttatcag attcagaaga
     1441 gaatgtcaac aacttctttg tttatttttt ctaaacgtct ttgccactca ttagcagcga
     1501 cttgattatc ataccatctt tattgttatt tgtcaataac tttttcgaag tttttttcga
     1561 taaaatcaag ctttattagc tatctcatcc aaacgacttc gttattataa catgacatca
     1621 aaagtgcgtc aacactattt tttataattt ttagtgacac cttaaaggaa tcatttagaa
     1681 caaataataa tatagcaatc tctttataaa attgcaatag aaaattccct atttttattt
     1741 attattttta aacacgaata ttacagcatt ttattgtttt ttcgttcatc taaaagatac
     1801 ttacatttaa ttaacggatg ttattctatt taattgttcg ctagtttttt tataatgaca
     1861 tccctgaatt tctcacttgc ttgtattttg taccttattc tttgttggat atataaaaaa
     1921 acaagtaaag cacatgcttt acttgtcttc tgcctttgcg ataggaataa agattctaaa
     1981 aattgtccct tgccctacta cactttctgc taagatacgt cctttatagt tttcaaccag
     2041 ttgtttagcg atagacaacc ccagaccatt tccacccttc gttctagctc ttgctttatc
     2101 cactcgataa aaacgattga agatcttatt aaggtcttct ttgggtattc cttcgccaaa
     2161 gtcctgaatc gcgatctcaa attcattcag agtggaagaa atagagatat ggacttcttt
     2221 tcgtgtagtt gaatacttta ctgcgttatc caatatgata atgattaatt gttcaaagtg
     2281 attacgataa atttgcaggg tcacttcttt tgtcaaatca tcatctaaag tgatcacata
     2341 gtctggatat aatatcttga aattattata aacttgatag gtgacttctt ttcctttagt
     2401 tgtttcattc gcataatgaa catctacttg ttctgctctt gataagtcta gcatctcttg
     2461 aactaagctt ttcatacggc tgatttcttg catgctggcg cttaatgatt cttctaatat
     2521 ctcaggatca tctttccccc aacgatttag cagactaaga tgcccttcaa taattgcaac
     2581 aggtgtcctt aactcatgag atacgtcttc cacgaattgc tcttgttgtt caatatacaa
     2641 acgcatacgg tcaagcatct cattgaatat ttcagctaaa tctgccaatt catcatttgt
     2701 gtcgatttca ggcatgtgga tatctgattg cggatcttta cgtatagtat ccattgtatc
     2761 tctgagtacc ttgagtggtt ttaagaaata agaagataaa aagaaaccta aaatactact
     2821 taagatcaaa gaaacaactt ctaaaacaat aagagtcaat aatagcttat ttcgaatatc
     2881 atagaaagaa gataattcat agaagctttg cgcatagccg atcttttctc ttgtaccttt
     2941 agaatatata ggctggatag ataaaaagcc agtttttcct tctagtgtaa cgatcgttgg
     3001 aactttacgt gtagtctgaa tcaggtttcg actgttttca tgggttttaa aaatcaagtt
     3061 ctcgtccaaa tcataaacat aaagatccat tgatggctgc cctagctcag ctataaagga
     3121 atcgatcttc attaagtttc cttcgaccag tgaagtatga tctacgacct catctccgtc
     3181 atttgtcgct ttcgtcagat tacgatacgt atttactaat gtcagttctt catccgagtt
     3241 agccaaacga gaagctactt ctgaaatcgt acgctctaca ttgtttcttt cttttgccac
     3301 aatcagattg atagatgatt tataggtaat gacagcgaaa attgtaaaca caacgaatat
     3361 gaagaaagaa ctcgcaaagg cccacttgat tgtcaaggag ggccctttca gttctctttt
     3421 ttccgatttt ttcggcttta tcacgagcgc atcacataac ctgttccgcg tacagtttgg
     3481 atatagcttt cctccccggg aacgtcaatt ttgttgcgaa gataacgaat atatacgtcc
     3541 actacgtttg tttcaacttc tgtttcgtat ccccacactt tatttagtaa aacgtcacgt
     3601 gctaatacca cgttcacgtt ttccattaaa gtaagcagca actcgtattc tctttttgtt
     3661 agttcaatta cttcatttcc gcgacgtacg acacggtttt ctttttcgat agtcaaatcg
     3721 cgataagtaa tagtagtttg tttagccaca tttttatcgc cttcaatatc gattcgacgc
     3781 aacaatgcac gaagacgtgc taataattct tcgatagcaa atggtttgac gatgtagtca
     3841 tctgctccgt ggtcaagtcc agatacacga tcgatcacag aatctcttgc agtcatcata
     3901 atgattggtg tatttttgac ttggcgtaca cgtctgcaga cttctaaacc gttcaattct
     3961 gggagcatca gatccaacaa tatggcatcc cactcattat ttaatgccgc atctaaacca
     4021 gttcggccgt tgtagtggac ttctgcgtta tacccttcat gcttcaactc tagctcgaca
     4081 aatcgtgcca agttcttttc atcttcaatg attagaatat tgctcattta ttattttatc
     4141 ctttctattc tactggcttt ttaaaaactc tatcattata ccgataatat gcttaaaaat
     4201 ctatagtcat tttatcatgg atagcaattt taaaaaagag ctgaagtcta ctcataacaa
     4261 tcgacttcag ctctttcgtc ctctttttat tttctcattt cctaacagcc gaaagactat
     4321 tattcttcat ggtaccatga gtaatggaag attccttctt tgtcgacacg ctcataggta
     4381 tgtgcgccaa agtaatcgcg ttgggcttgg attatatttg caggcagacg ttctgctctg
     4441 tatgaatcga agtaagcgat ggctgatgag aaagtcggta caggtacgcc agcttggaca
     4501 gccaaggcaa cgacgtctct gactgattgc tgatattttt tcgtgatttc aacgaagtaa
     4561 tcatctagca ataaattttc gatctcagga tttttgtcat aggcatccgt aattttttgc
     4621 aagaatcgag ctcggatgat acatccagcg cgccagattt ttgcgatttc accaaatggc
     4681 aagtcccaat caaattcttt tgaagcgaca cgtaattgag caaatccttg ggcataactc
     4741 atgattttac tgaaatacaa cgcttctcgg attttttcga ccaattcttt tttgtctccc
     4801 gtgaactcaa aatcgttcgt acgagacaaa atcttgcttg cttgtacgcg ttcttcttta
     4861 taagcagaaa tatagcgggc aaatacagat tcagtgatca atggaagtgg cacacctaag
     4921 tcaagcgcgc tttggcttgt ccatttacct gttcctttat tcccagctgc atcaaggatc
     4981 acatctacta ctggtttacc tgttccttca tcgtctttac gtgtcagaat gtcagcagtg
     5041 atttctatca aatagctgtc taattcgcct tgattccatt ctttaaagat atcagccatt
     5101 tcttctactg aaaggtttaa aatattcttc attaagtcat aagattcagc aatcagttgc
     5161 atatctccat actcaatacc gttgtgtacc attttcacat aatgtccggc tccatttggg
     5221 ccaatatatg tgacacatgg tgctccgtct tctgcttttg cagaaatttg ctctaagatt
     5281 ggtgcaacta gcgcataggc ttctttttgt ccaccaggca tgattgaagg tccttttaaa
     5341 gctccttctt caccaccgga aacgcctgta ccgataaagt tgatgccaga gttggctaac
     5401 tcttcattac gacgcatcgt atctttaaag aatgtatttc ctccgtcaat caaaatatcg
     5461 cctttatcaa ggtgaggaag taactcttgg attgttgcat ctgttgccgg tcctgctttg
     5521 accattagca agatccgacg tggtttctcg atagattcta caaattcttc gattgtatat
     5581 gttgctttga aatttttatc tgggtgttct tctactacag cggttgtttt agcccctgta
     5641 cgattgaata aagccactga atagccgcgg ctttctatgt tcaaggctag atttttaccc
     5701 ataacggcca taccgacaac gccgatttgt tgttttgaca tatgaactcc tcctacatac
     5761 gggatttctc cctaatctct tacattatag cttaacacgg ctacattgca aactaaagaa
     5821 caacatattt tttatttacc gactacaaaa aaataaacct caaggctcca tggttagcct
     5881 tgaggtttta cattttctaa ttaaaatgta ttaagcttct tttgacatta cgtccttacc
     5941 atcatagtaa ccacaatttg cacatacgtg atggcttttt ttcatttcac cacagtttgg
     6001 acattcgttt aaccctttga ttgtcaattt gtagtgtgta cgacgttttg cttttttagc
     6061 ttttgatgtt cttctagctg gtactgccat tttgctacac ctccttgtaa agtcttgtat
     6121 ctaaattaga cacaatatat tccaatctta cttgttatcg tcgtcttttg atttagaatc
     6181 aaaaagttca gatagttttg caagacgagg atcaatttta ttctcggctt cttccatttt
     6241 tctgcgctgg tattcttctt cggaaacaac ttcccaatca ttgccttttg gaagttctgt
     6301 tgctgcttcc tcttcttttg tcaatacccg cattggaatc gccaataaga tgttgtcttc
     6361 aatcgactct gctaaattca ataatggacc ttcaattagc aaaatctcat ctgcagacat
     6421 ttgttcatct ctactttgga attgttcttc tgtcatgaat acttcgtcta cagaaaaatc
     6481 catgggcagt tcaacaggtt ctagtgaacg tgttgaagga actgtcaaag tcgtctgcac
     6541 agtgtaatga acgatatagc ctttcttatc aactgagacc aaaccttcga cttccactgg
     6601 cgcaacatca agtattaaat tgtctcgctt catgagagcg gctttcaagt cgagcgtctc
     6661 atgaaaagtt aacggtgtct cctggtattt tctcaattcc aataaagacc atttcatttt
     6721 tcatcactcc taagcaacaa aagcaattat acagggcaaa aagcatgttg tcaatgaaat
     6781 tttcttgaca ccttgcagtt cataaaaaaa cagaaaaatc tttattagaa agcctctgtt
     6841 tgctatcatc ctcagaatca tttacaataa tcattattcc attaaaggag cggatgatct
     6901 tcatgaacac gaaagatatc gaatactatg tccaaatcgt caaacaaaaa aattttacga
     6961 aagtggctaa ttatttccgt gtgagccaac ctactattac atatgcatta caacgtcttg
     7021 aaaaagagtt tgacaccgta cttattacaa gagatcgatc gcatcacgaa ttgatcgtca
     7081 cgccttctgg aaagcagctg tttctccatg cacaaaccat tctccaagag atttctactg
     7141 caagagctga aatcactcag ttgcaaaaga aaaagcttcg ttttggcatg cctcctatta
     7201 tcggcaatga atactttcct aaactgtcaa gttactttat caagcatgaa ttgatgaatc
     7261 aaattgagat taccgatgga ggctctcggg acttatacgg gatgatccga caagggagga
     7321 tcgatttagc tgtattaggc tcaacacagc caatcgtgga tcaatattta gaaacagagt
     7381 tacttttgga caaacgtttc atgatcgtcg tgccacctac acatcctctt tccaagcagg
     7441 atactgtctc tttcaaagat atagcagatg aacagtttgt actattgaac gaacattatg
     7501 tccaccccgc agcattcact aagctttcta aacaaacaca tactcatcca aaagttgttt
     7561 atcaaagcaa tgacttgacg attttaaaga gtatggtccg agaaaatgtg ggtatcggtt
     7621 ttttggcaga gattgctgtc catcctgaag atcatttagt ttgcctccct ctttcagatg
     7681 aagttcagcc tcgcttcttg atttcattgg caaaacgaac acagcagatc gtcacgcctc
     7741 ttcaagaaca agtagtccaa gttatccatg aattcgttac agcacaaaaa aacagctctt
     7801 cgtaaaagaa gagctgtttt tattttttat taagctaaat tagatagctg ttgtttcgcc
     7861 acggtaaacg atgccgcgac gtgaatcaac ggtaatcaat tcaccttctg taattgttgt
     7921 tgttgcgcct ttagcaccaa cgattacagg aatatcttgt gcaattccta caactgcagc
     7981 gtgagaagtc aatccgcctt cttcaacgat aacagcagct gctttttcga ttgcaggcat
     8041 atattctttg tcagttgatg gtacaactag aaccattccg tcttttgctt tttttacagc
     8101 ttcttcagca gatgaagcaa taactgcatt tgcaacgaca gatttttgtc caacgccttg
     8161 agcttctaat aatttagaac caatcatttg aattttcatc acgttagttg ttccgcgttc
     8221 accaactggt acaccagcag tgatcaagat taaatcgcct tctgatgcga aacctaattc
     8281 aacagctttt ttagctgcta gttcaaacat ttcatcagtt gttgatggtt tttcagcaac
     8341 tgttgggtaa acgccccagt ttaacatcaa accacgtttt gtgcgttcgt caaaagtaac
     8401 agccaagata tctgcatctg gacgatattt tgaaatcatt tttgcagtgt atcctgattc
     8461 agttgcagca acgattgttt tcacgcctag attttttgca gcacgagcta cagataaacc
     8521 gattgtttca gttacatctg ttttatcgaa ttcgttcaat tggaatgtac ctaattctga
     8581 taatgcattt tcagcttctt ggtcgatacg tgccattgtt gaaacagctt caactgggta
     8641 gtccccgttt gcagattcac cagatagcat tgttgcatct gtgccatcaa atacagcgtt
     8701 agcaacgtca gaagcttccg cacgtgttgg acgtgggttt tcttgcattg attctaacat
     8761 ttgtgtagca gtgataactg ggataccagc agcattacat tttttgataa tgcgtttttg
     8821 aaccattggt actagttcag ctgggatttc aacacccata tctccgcggg caatcataac
     8881 accgtcagaa actttgatga tttcgtcaat gttgtcgata ccttcttgag attcgatttt
     8941 agggaagatt tgaacatgag tcatgtcttt ttcttctaag atttcgcgga tatctagaac
     9001 gtcttgtgct ttacgtacaa aacttgcagc gataaagtcg atatcgttgt ctaaaccaaa
     9061 acgaatatcg tcagcatctt ttggagtaat acctggcaaa ctaatagaaa cgcctggtgc
     9121 atttacccct ttacgtgatc ctagcatacc tgcattttta acttctacga ctaattcacg
     9181 gtttgcttcg tctttttcaa tgatttgcat atcgatcaaa ccatcatcaa ataaaatgtg
     9241 tccgccaacg tgaacatcgt caaataatcc tgggtaagta accgcgattt tttcttttga
     9301 acctacatgt tcaggatcca tagagatgcg tgtttgatct cctacttcaa atttgatgta
     9361 tcctgcacga ccaaaatcag cttctgttgt gccttgaaca gttgtacgga tttcagctcc
     9421 ttttgtatct aaaagaatac cgacatcttt tcctgtcttt ttaactgctt cacggaccat
     9481 agtcatacgt gataattgtt cttcatggtc accatgagaa aaattaaagc ggaagacatt
     9541 tgccccagat tcaatcaatt gagaaataat ttctactgag ttacttgctg gtcctaatgt
     9601 actaacgatt ttggtttttt tcatttaaaa aactctccca ttcaattttg tttatttaaa
     9661 gctgtacaca gcttaaatag cttggtttct ttcactctta aaatgaaagt gaattattta
     9721 gatcatatag tgataagtct ggtttatgtt tgttgttttc aagtgtatca atgatgtcag
     9781 acgcaacgat ttcgttgtca tggataccga cacataatcc accttttcct tcttgaagca
     9841 attcgacagc gtaagcaccg aatttacttg ccaacacgcg gtcgcgtgcg cttggtgctc
     9901 caccacggac aacgtgtccc aagatagata cacgtgtatg atagtcacca aattctgaaa
     9961 gtttctcagc gaattcattt ccgcccatta caccttcagc caagatgatc aagcaatgtt
    10021 tcttcccgcg atcgcggcct tcttggattt ttttagcaac tgaagccata tcaaaatcat
    10081 gttctgggat gatgatttca tccgctccgc cagccacacc tgaccataaa gcgatgtctc
    10141 ctgcattgcg tcccattact tcgataacga atgtacgaac atgagaagtc gctgtgtcac
    10201 gaatacggtc aattgattct agtactgtat tgattgctgt atcaaaaccg attgtgaaat
    10261 cagtcccagg aatatcatta tcgattgttc caggaatacc tactgctggg aaaccgcgtt
    10321 ttgtcaaagc cattgcgcca tgataagagc cgtctcctcc gataaccact agaccttcga
    10381 ttccgaattt tttcaattgt tcgattcctt ttaattgacc ttcttcagtt gcaaattctg
    10441 gataacgtgc agaatacaag aatgtaccac cacgttggat cttgtcacct acatctgcca
    10501 catcaagacg acgaatatct ccagcaacta aaccggcaaa accataattg atcccgtata
    10561 cttccagtcc ttcgtaaata cctttacgaa caacggcacg aacagctgca ttcattccag
    10621 gtgcgtctcc gccactggtc aaaattccga tgcgtttcat aataaacatt cacctcatta
    10681 aaatacttta tcctaagtat gacttaggtt tacaaccatt tttgcgttaa ccacgactaa
    10741 cattctaaca ttatttttct cacttgtctt gatttacgga taaaaaaatg aaaaaaaatg
    10801 aaagcaagta gtatttttta ccgtaagtta tttgaaaacc acattatctt cgccaagcag
    10861 ataaataagt tgccgcttta tactcttgct gtcatccacc caataagcat ctgacagaac
    10921 tacttttcgt tgcgtttttt cgtgatacat aactactggt atatatcctg ggtgtttctg
    10981 aaaaatcact tgtaattgtt tcattatctc gggatcttcc cgttcttctg taatacgcaa
    11041 atagcaagtt tgtgaaggat actgggcttc taactggtta ggatcagcaa agctttctcc
    11101 aattagttgc agttcctgat tatatttgct gatttcgctc cgcccttgaa caaagatcac
    11161 tcgatcaacg gcaaacaaat tacgatattt acgataaaga gttggaaaaa ctgtgacaga
    11221 aatttctcca ctactgtcgg ttccttcaac aaaggccatc agttctcctt tttttgtccg
    11281 aatttctcta acttccttga tcatcactaa taaattgact ggttgattag ggacgatatc
    11341 tgtaatcagc tgaacttgtt tagctaaacg aagacgatca aacccttctg caggatgacc
    11401 tgacaaataa atacctaagt actcctcttc cagcttcaag cgttcttcta gtgtatagtc
    11461 agcgacttct tcttctttta atgccatgat tcccagcaag tcaagacttc caccactgta
    11521 aacgatattt tggatttttc cttcgagatc aagcatcaat tgtttcctgt taggagataa
    11581 ttcatcaaaa actcctactg ctgtcagtga ttgcagtaac tctaatttca gccaacgctt
    11641 atcgatccgt atgaggaatt gatccactga ctggaacggg ccattttgtt ttctttcgtt
    11701 taatatatct tctacaaaat cacgccgaat accttttatc gctgtcagtc cgaatctgat
    11761 ttcttcctta ttgaccaact caaaactgga attactttga ttaatggacg gcgaggaaag
    11821 tttgagttta tttttacgtg cttctgcaat atattctttc aattttgatg tgttatgccg
    11881 aacagaattc atcaggctgg taaagaaagc acctggatga tgggctttta gatacgccat
    11941 ttggaatccc acgaatgaat aagcaaatgc atgtgaccga ttgaatccat agtttgcaaa
    12001 tttttcgata tattcatata cggtcgctgc tgttttttcc gaatatccct gttgtaatgc
    12061 tcctgataca aaatgcgact tttcttcatc taatacgtct tttttctttt tactcactgc
    12121 ccgtcgcaga atatctgact gccctaatgt aaatccagcc atagtcgagg cgatctgcat
    12181 gatttgttcc tgatagacga tcactccata tgtgtttttc aaaatcggga tcagtgaatc
    12241 atctgggtag cgaatggctt cttttccttt cttcctggca atgaaggtat ctatattttg
    12301 catagggcct ggtcgataca atgcattgac tgcggcaata tcttcgatgt tttctggatc
    12361 taatcttcgt aaaacgttcc ggataccagc agattcaaat tgaaatactc ctgaggtttc
    12421 gcctcgttga aacaagagca gcgtttttgc atcatttagc gggatctctt ttaatcggat
    12481 ctcataccct tctactcgct taacagcttt caaagtaaaa tcaataatcg ataagttgcg
    12541 tagtcctaaa aagtccattt tcaaaagacc gattgcttct acctcattca tcgtatactg
    12601 ggtcagaaaa gcttcatcag aaccgcgttg gagcggaact agttcaagta gattcttatc
    12661 gctgataact acacctgctg cgtgagtcga aacatgtctt ggcaatcctt ctaaaatcac
    12721 tgcagttttg aataaaagtt ggtttttttc gcttgcctga actaattcta ctaattgatg
    12781 cgaagtttta taagcttggg tcaatgtgat tttcaattga ttgggtattg cttttgacca
    12841 gcggtttgct tcactttgcg ataaaccaaa cactctagct gcatctctaa gaaccatttt
    12901 tgctgccatt gtgccaaatg ttgcgatttg tgccatatga tattgtccat acttttctcg
    12961 aacataattc aatacttctt cacgtcgatt gtcaggaata tccaaatcga tatctggcat
    13021 cgtaaagcgt tcggggttta aaaagcgttc gaacagcaaa tcatattcta atggatccac
    13081 atctgtgatt tctaacacat aggctactaa tgaacccgca gcagaaccac gacctgcacc
    13141 ggtaacgatt ttgtttctat gagcaaaagc cattacatcc catacgatca agaaataatc
    13201 atcgaatccc atttgatgga tgacagataa ctcgtagtcc aaacgttctt ggtattcctt
    13261 tgtatagtca gtgattcggc gttccacttg cgaatagcat aactctctta aatattctcc
    13321 agcttgtttt ccattaggaa ctggataatg cggaagtaat ttctgatgga actggaattc
    13381 aaatgaaatc gattcagcta aacgctgggc gcctattaca gcttgggaaa gacccgcttt
    13441 ttcgaatcgt ttgatcattt cttgttctga aagaagatag acatttgctg ttagtggtaa
    13501 agatgcagat aaattttcta aaggtatttg gcttccctct ttgatatgtt tcatcacttc
    13561 aagtgcaaaa agttcttctt gttgtaaact agcgatggga tgtaatgcac aaagcgaata
    13621 attttctttt atcacatact gataccattc aggcaattga tcaggcgtgt tttctgtcaa
    13681 gccgatataa aaatttctag taaaaacatt ttcaagtaaa aactgccaat gaccgaaaaa
    13741 gttatcctct ttattgaaag cctcagaaac agaatgattt gccggaacaa caaacgctag
    13801 atggtcggtt tctgtcattt ctgcaagttc caatttttct tggatcattt ttcggctgga
    13861 aagtttcatc agctgctgat aacctatttc atcttttgcg tacgcaagca agccataatt
    13921 tttttcttca taagtataac taagatgtaa tccaatgatt ggctgtactc cttcttctac
    13981 acaaatacga tagaattcag gtgcaccgta taagacatct ttatccgtta tggctaatga
    14041 agtgtagcct tgtttttttg cttcttgtac atattctcga atacgtatcg tactttgtaa
    14101 aagagaataa gaagtaattg tataaagttg tgggaaaaac atcttgcatt ccgccttttt
    14161 tttcgttaaa attcactagc gttaaaaggt ttgatccttt tactttcatt aagatagcag
    14221 gcgctgaagt tgattattcc acttcatttg cttgcttgta atctgtagga atgagttctt
    14281 tcgcaagaac tcaaatttta ttgagatttg gtctcgtttc tttacattca aagaaaatct
    14341 ggtaaaatta agatagaaaa gaggtgttct ttatgcgata tatcgttaca ttattctggg
    14401 ctgtcgttct aggtcaagta gtcggttata ttggtgctgc attaacaagc ggaacatatg
    14461 attttacttt gacaacaatc atttcattca ttgcgggtgt cattatcctt ttgattggtg
    14521 cagttgcgcc aagaaaagaa acctctgccc attcgtaaaa aaacagccaa gagcttgaga
    14581 tttctcaagc tcttttttat tttctactcc tcaccaccat tataatctgc tgcgaagaac
    14641 tttacgaaag ttgaataaag aaagaagaat atttgacaga acaaacaaac ttgtcacaat
    14701 aaaaacagca ctgtaattat aagcatgcgc gactgtcgat cctagcatgg gacctaaaac
    14761 ttgtccaaag ttgctaaaca tctgattgta gctatagatt cggctgactc cttcttgagg
    14821 cgtaatctta ctgatcagtg tattgacaga aggcatcaaa gcgccagttg aaaagcctaa
    14881 aataaagcga agtataccta attggagcgg tgtagtgacg aatgccatag gtaaataaca
    14941 gcaaaatgat aaaatgagtc ctccaattag aactttttga ttcccaatct tatcccctag
    15001 ttttcctagt ttcggtgaag aaaagacagc ggaaacacca gctatcgaaa caatcaagcc
    15061 gctgacaaaa aggatatttt ctgtacttcc gctcaattca cgaatatata aggtcaaaat
    15121 agggctgatc gtagtgattc ctatctgtaa aatcaaggtc gtaatgaaga gaccgatcaa
    15181 aatagagaga tgatccattt ttgaaaaaat ttctttggtg cttatcagat cttttttctc
    15241 gattggatgg aaatcttctt tgactaagaa aatggtcaat accgttgtga tcatcaacaa
    15301 agcccctgtg atcaagaata cattttccat accaaaccac tgagccagtg caccgcccat
    15361 tgaaggtccg atcagatttc cagcaatcgc tccggtagac aatgttccta acgcccatcc
    15421 gctcttttct ctaggtgcct gtgaagcaat catcgctgtg gcatttggaa tatatccaga
    15481 caaaatccca ttccaaaacc gcatgatcaa aagccaatac gcgtttggta caaaagccaa
    15541 agatcccatt gttaatgtca tccctgctgc tgcacggatc atcatgagtt ttcggccttt
    15601 tcgatcagct agatttcccc agattggtgc aaccacggca gatgcaaaag cagtcacaga
    15661 gattgctagt cccgaaaaca attcgatttg atcttttggt gttcctagtt gctcaacata
    15721 aactggtatg aatggcataa ctaaactaat acttgcacca gtgaaaaaac agccaatcca
    15781 cgcaatcact aaattttttc tccaattaat ctccatctat catccctctt tcgtctcttt
    15841 tatcatacct attttcgaat aaacaagcaa cgatctcgcc agtaaaaaga gaaaaatccc
    15901 gcaaaggtca ttttatatgt aacgttcttt ttatgtaaaa caaaaagaga tcagaacaaa
    15961 tactgttcca atcccttctc tttagttaaa ttgttgctag aatgatgaag ttgataatga
    16021 ataaaatatc aacgatccac aaagcaacag aaacttgatt gtattttcct ttgattactt
    16081 tcacaattgt atagaaaata aatcctgctg caatcccata ggaaatgcta tagcacaatc
    16141 ccataaagat cgatgcaaag aaggccggta aagcttcttc catatctagc catttgatgt
    16201 ctgcaaaaga agccatcatc attactccaa ctaaaatcaa tgctggcgca gttgcttgat
    16261 ttggtacaat ggcgatcagt ggagagaata aactacttaa tgcgaatagc actgccacta
    16321 ctactgaagt caaaccagta cgtccaccgg ccccgatccc agctgcagat tcgacataag
    16381 ttgttgtatt cgatgtacca aagatagccc ccacagaagt agcgatcgca tcggcaaata
    16441 gtgctttatc catttttgtt ttgaacccac gaccatcttc caacgcgatc tcgtcttctt
    16501 ttgagaaaat acctgtgcgg cgtcctgtac cgataaatgt tccgatcgta tcgaaagtat
    16561 cagataaact gaatgcgatg atcgtcatca atacttgagg aatcttggaa gaatcactga
    16621 ataaagattg catgccttca gaaccaaatg ctgcaccgaa agttgttcct aattctttaa
    16681 atgaattacc taacgaattc gtttgccaat caatggaact taaatcaaca acacccataa
    16741 aaataccaag gactgtggtc gctacgatac cgataagaat agccccacgg acatttttta
    16801 caactaaaat ggctgtcagt accaaaccaa tcacagccaa taagatagga gcgttgtcaa
    16861 agttggctaa cgcagggacg attccaccat ttatcgtaac atttgtcgct tttccgcctt
    16921 caacgacaga gctcgaaata gctgactggt ctgctgaaaa agataagaat cctgcatttt
    16981 tgattccgac atacgcaacg aaaataccga tcccgccacc aatcgcatgt tgaagacttt
    17041 cgggaatcgc ccgaataatc atttttcgaa tatttgtgac agtaataaaa atattgatta
    17101 atccgcaaat aaacaccatt gccaaggctt gttgccaaga ataaccaagc ccaaaaacga
    17161 ccgtaaaagt gaaaaatgcg ttcaatccca taccaggtgc ctgagcatat ggtacatttg
    17221 cgaacaatcc cattaccagc gtaccaataa tcgatgcaat gattgtcgct agaaatactg
    17281 cttggaaggg catacctgat gctgacaaaa tcgtcggatt tacgaataag atataactca
    17341 tggcaaagaa tgtcgttact cccgccatta tttcagttga tacatttgta ttgtttttct
    17401 ttaaaccgaa aaacttttcc ataaaaagtt ccactccctg aatttttttc tgatggaaaa
    17461 ctataataat aaaaaataag taaaataggg caaatccatc gaacaatttc aaattatata
    17521 gacgtttcat tcgcttttca acgttttttt aacaaaaaaa gcgaaaataa tacttaacgt
    17581 tcgtgtttag cttatgttta gctatttata tagccataac tgtagttttt gttaggaata
    17641 gctgattgct cttgttaatt acctaaaata tgctagacta aaacaaatgc tagttgagga
    17701 aggaaggacc cttattgaat aaaaaattaa ttgccatcga tctggacgga actaccctca
    17761 atcaagattc gatgattaca tctaaaacaa aagagactct gaaaaaagca atcaatgccg
    17821 gtcatcatgt cagtattgct acaggacgcc cattccgaat gagccatcag ttttaccaac
    17881 aattagaatt aacaacaccg atggttaatt ttaacggcgc attagtacac atccctaatc
    17941 agcactggga tggtgaaaga gaaacgttga tcaatcgtga aatcgctttt gaaatcttat
    18001 cgcaaaaaaa gcagctaaat ttagatttta tcgctgctga aaatcgtgat acttttttta
    18061 ttgacagttt tgatttcttt gatgaaaaaa tttttgcttc ttctcgtcca ggagagaaga
    18121 atttgctttc tcctaaaaat ttgacgacaa atcctacatc tttgcttgtc cgaacagaca
    18181 aacgttttgc aggagctgta tctgctgaat taacacgtca atttggcagt tatgtcgatg
    18241 ttcggacatg gggtggacct actgccatct tagagattgt ttcaaaagga atccaaaaag
    18301 caaaaggcgt gcaagagatt gctaactacc tgtctatcga tcaaaaagac gtcattgcct
    18361 ttggtgacga gcataatgat ttggaattgt tggattatgc aggttgggga gtagctatgg
    18421 ctaacggtac tgaccaactc aaaggcattg caaatgatgt cactcctttg agcaatcagg
    18481 aagacggttt agctgtttat ctggaaaaac tacttaaact ataatagaga attagattcc
    18541 acaaaaaaga ggttgtgaca atttttgtca caacct
//
LOCUS       DACGLW010000059        18123 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.061, whole genome shotgun sequence.
ACCESSION   DACGLW010000059 DACGLW010000000
VERSION     DACGLW010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18123
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.061"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            complement(62..601)
                     /locus_tag="IXT53_001981"
     CDS             complement(62..601)
                     /locus_tag="IXT53_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HAQ4905782.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(741..1352)
                     /gene="rpsD"
                     /locus_tag="IXT53_001982"
     CDS             complement(741..1352)
                     /gene="rpsD"
                     /locus_tag="IXT53_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HAQ4905783.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            1668..2465
                     /locus_tag="IXT53_001983"
     CDS             1668..2465
                     /locus_tag="IXT53_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-nitrite transporter"
                     /protein_id="HAQ4905784.1"
                     /translation="MNPVSPLFEKIDASIEKKANLFENSFSRYAVRAVLACLFLTLGT
                     AVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAELGTSNMLYMTVGVFRQRLA
                     LSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNFMLESIAVKLAKPSATILV
                     EGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLGYEHVIANFPAFTLTYFAS
                     HGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQSKSSYVD"
     gene            2493..3128
                     /locus_tag="IXT53_001984"
     CDS             2493..3128
                     /locus_tag="IXT53_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HAQ4905785.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            3148..3921
                     /locus_tag="IXT53_001985"
     CDS             3148..3921
                     /locus_tag="IXT53_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ4905786.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            4329..5126
                     /locus_tag="IXT53_001986"
     CDS             4329..5126
                     /locus_tag="IXT53_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HAQ4905787.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            5104..5517
                     /locus_tag="IXT53_001987"
     CDS             5104..5517
                     /locus_tag="IXT53_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905788.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            5501..8146
                     /gene="mutS"
                     /locus_tag="IXT53_001988"
     CDS             5501..8146
                     /gene="mutS"
                     /locus_tag="IXT53_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HAQ4905789.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            8164..10272
                     /gene="mutL"
                     /locus_tag="IXT53_001989"
     CDS             8164..10272
                     /gene="mutL"
                     /locus_tag="IXT53_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HAQ4905790.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRQTGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            10294..10854
                     /locus_tag="IXT53_001990"
     CDS             10294..10854
                     /locus_tag="IXT53_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HAQ4905791.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(10939..12927)
                     /gene="treP"
                     /locus_tag="IXT53_001991"
     CDS             complement(10939..12927)
                     /gene="treP"
                     /locus_tag="IXT53_001991"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HAQ4905792.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            13150..13863
                     /gene="treR"
                     /locus_tag="IXT53_001992"
     CDS             13150..13863
                     /gene="treR"
                     /locus_tag="IXT53_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HAQ4905793.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            13940..14386
                     /gene="msrB"
                     /locus_tag="IXT53_001993"
     CDS             13940..14386
                     /gene="msrB"
                     /locus_tag="IXT53_001993"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HAQ4905794.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            14556..15158
                     /gene="ruvA"
                     /locus_tag="IXT53_001994"
     CDS             14556..15158
                     /gene="ruvA"
                     /locus_tag="IXT53_001994"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HAQ4905795.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            15171..16172
                     /gene="ruvB"
                     /locus_tag="IXT53_001995"
     CDS             15171..16172
                     /gene="ruvB"
                     /locus_tag="IXT53_001995"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HAQ4905796.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            16201..16779
                     /locus_tag="IXT53_001996"
     CDS             16201..16779
                     /locus_tag="IXT53_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAQ4905797.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            16831..17313
                     /locus_tag="IXT53_001997"
     CDS             16831..17313
                     /locus_tag="IXT53_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HAQ4905798.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            17430..17804
                     /locus_tag="IXT53_001998"
     CDS             17430..17804
                     /locus_tag="IXT53_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905799.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         6020 a         3253 c         3941 g         4909 t
ORIGIN      
        1 ttaaaatagg gcagcatgga agagactgaa tgttgagtct tcttccatgc tgcttttttg
       61 attaatttgt atgaagaaat cgatttagcc gttttttcat caagataccg atataagctg
      121 ctccagttgc cttgattgct tccggcagta aaaacggcag tagtccactg gcaaatgcag
      181 ttgtaaaagt aagatcacca cttattttca gccagatcgt tccgcagatc aatgcagcaa
      241 gagagcccat tagattggca aagattgcat ataagtaatg agcatccgtt ctttcgatca
      301 tccaaccagt tatccacgtt tgtaagagaa atccaatcaa atatccgcca gttggcccaa
      361 ataaaacagc tatacctcca gtcatccctg aaaatacagg aagaccaatc aagccaagta
      421 aaagataaac tccaacagat atcaatccta gttttcttcc gagaaaagtt gctgttaatc
      481 caatagcaaa tgtttgccct gtcaaaggaa ccaaaccaaa aggaatggtc atctgagaaa
      541 ggacagaaat aatcgcggca aattccgcag caagtatcaa ttctttcaat gttcggttca
      601 taacgccctc ttttcgttaa cttaatattt ttatttggtt aacgaaattg taaaacaaag
      661 tgattacttt gtaaatagaa aatataaaaa aagaccagct atccaataga tagcggtctt
      721 tttcaagaaa gcttttgaaa ttataagctt tggttgtagt attcaacgac aagagcttcg
      781 tcgatttctg gatatagttc gtcacgttct ggtaaacgag tcaatgaacc ttccaatttt
      841 tcagcgtcaa agcttacgaa tgctgggcgt ccaacagttg cttctacagc ttcttttact
      901 gttacgatgt tttgtgattt ttcacgaact gagatcactt gacccacttc tacgtggtat
      961 gaagggatat caacgcgttt gccatcaact aaaatgtgac catggttaac taattgacgt
     1021 gcttgacgac gagtcgttgc aagtcctaaa cggtaaacaa cattgtctaa acgttgttct
     1081 agtaagatca tgaagttaac accatgttta ccttctttga ttttgctagc ttttacgaac
     1141 aagttacgga attgacgttc gttcattccg tacatgtgac gaagtttttg tttttcagtt
     1201 aattgcatgc cgtattctga ttgtttgcca cggctattag gtccatgttg acctggtgcg
     1261 taagggcgac gtgctaattc ttttcctgtg cctgacaatg agatgcctaa gcgacgagaa
     1321 attttccatg atggtcctgt ataacgagac attaaaattt cctccaatga atataagttt
     1381 ttggagtaaa ataatccgtt gaaaaattca taatcatata gtttgttctt caatcttcac
     1441 ctttgcagcc gcggttacac aatcgaaccc gagacggggc aacaaactag ggatgagtta
     1501 ttatttttct gctgcattat tttacacaaa ttacagtata tgatagatca tccacgaatg
     1561 tcaagataat tagtacataa aaacgcttta caaacctagg ctgtctttat aaaatgagag
     1621 ggtatgtgca aatggaaaaa agcgaaggaa gtttaaggag aaaatcaatg aatccggtat
     1681 caccattatt tgagaaaatc gatgcttcaa ttgaaaagaa ggcgaatctt tttgaaaaca
     1741 gcttttcacg ttacgctgtt cgagcggttc tcgcctgttt gtttttgact ttaggaacgg
     1801 ctgtggcatt tgcgatcgcg atgaaggggg aagatatcgc gcatggtcta ggaaagattt
     1861 tatacgcatt tatgttcagc tggggtctgg tcatgattct atacatgaac gccgaattag
     1921 gaacatcgaa tatgctttat atgaccgtgg gtgtttttcg gcagagactt gctttaagta
     1981 aggcagccaa gattctattt gcctgtattt ttttcaatat gattggcggt atattatgtg
     2041 gatttctaat ttctatgact acgccatttc aagatttaac gttggacaac tttatgttag
     2101 aatcaatagc agtaaaacta gcaaaacctt cagcaacgat tttggtggaa gggatgtttg
     2161 cgaatatcgt tgtcaatacc gctgtactga tcagcatgcg gatgaaagag gatgtcggaa
     2221 aagtgatcac agtcgtattc atcattttca tttttgcttt tttaggatat gaacacgtga
     2281 tagcgaattt tcctgccttt acgcttactt actttgcttc tcacggacag atggatggaa
     2341 tgacagtcag caatgtgtta cataatttgt tttttgcttt ggcaggaaat tatattggtg
     2401 gcggtttagt aatgggactg ggatatgctt ggctgaatca gtcaaaatca tcctatgtgg
     2461 actaaaaagt cgttcattaa aggggaagtg ccatggaagt tttagttttt ccaaaaaatg
     2521 aggaaaaagc gttacaaatg gaaaagtaca tgaaaaatct tttttccttt gctggagtac
     2581 ctaagccgga acgcgctctt ttacaacaac cgttactgga tgagagcaaa aatttatcta
     2641 tttctgaagt tttagctttt gttcattatt attatgaaca aatcgaaaga gagtatcagt
     2701 atgtagcgat cgatttagtc cagaaaaaca tcaaacggct tacatttgaa gacttagtcg
     2761 tattgttgcc actagttcag aaaaaagaat ggtgggacag catcgactct tggagaaaag
     2821 tatacagcac atggtgtaaa gcctacccag agcagcgtga agaagtcttt caattgtttg
     2881 aaaaaaagga agatttttgg ctacgtcgaa tagcaatcac gttgcaatta ggttataaac
     2941 agcaaacaga cacaggacta ttgatccgag caatagacga agatcgtcaa acaaaggaat
     3001 tctttatcca aaaagcaatt ggttgggcat tacgcgatta tagtaagacc gatgccgatt
     3061 gggtaaaaag acagctgaca acagatttga gtaagttagc tgttcgagag gcaagtaagt
     3121 atttataaga aagcgagacg acaagagatg agacgcaaat tatttgcttt tgatattgat
     3181 ggaacgttgt tagattcaaa caaacaagct ttagatagca caagagaagc attggaaaaa
     3241 ttacgcaaag ccggtcatct ggtcacgatt gctacagggc ggagccgatt tcatgcgcaa
     3301 gagatcattc gcgatcttga ttttacgaac tacattttat gtaatggagc ggccggtttt
     3361 ttagaccatg aacaagtata taagaattta ctagatcgcg accagttgga tgcgttcgtt
     3421 caagaagcgc atcatgagca gatcgataca gcttttgtca gcttagacaa tatcaagcgt
     3481 tcttcttcca atgatgtaac ggtgatggaa gaagcgatgc attctttcgg tgctgtcttg
     3541 cctgaattag atcagttttt tgcagaggaa caagaaatat accaagcgtt agcatttttc
     3601 gatcaaacat atgatgggaa gttctcttca tatgataaaa ttcgatttgt ccgctggcat
     3661 gagaacagtg tagatgtcgt acctaaaaat ggttctaaag cagcgacaat tctgcactta
     3721 gcagaacaag taggcatccc tcatgaagac atcatcagct ttggcgatgg acaaaacgac
     3781 cgtgaaatgt tgcgcatgtc aggaattggg atagctatgg ggaatgctgt gccagaagta
     3841 caagcagaag ctaagatggt cactgataca aacgatcagg atggaatttg gaaggcattg
     3901 aaagagttaa gcgtgattta aaagaggttg tgaaaaagct gttctgcatc aagtaataag
     3961 cccagccaaa caaaaatagc tcctcctatt tttcgttttt ctgggcttat taccgcagat
     4021 gcaagctttc gaacaccgtt taaggaagag gtcgtgacag aagtggtcag tttcaagaaa
     4081 taagaaggga tttacgaaaa ttgcttctca aatttttgta aaattacggc ttatttctga
     4141 agaagtagat gaaacaacgt ttattcggga aatgggactg tgacaagatg tttgtcatag
     4201 tcccattttt ttattttatt tttcaaaact aagaaatacc aggaaagagt tccttttgtt
     4261 tgaccttttc tttcttattg aaaacaacta taatatagag tgatgttaaa agagaagagg
     4321 aatttagtgt gcgggtattg tttattggtg atgtagtagg ttcaatggga cgagagatga
     4381 ttactgaata tcttcctaga ttgaagaaga agtaccgtcc gcaagtaacg attgtaaatg
     4441 gtgagaatgc cgcttctgga cgtggaatca cagaaaaaat ctataaaaaa tttttgcaag
     4501 acggcgtaga tgtcgtgacg atggggaatc atacgtggga caaccgcgga atctttgagt
     4561 ttgtaaatga agctaaaaag atggttcgtc cggcaaattt tccggaaggg acgcctggac
     4621 aagggatggt ctttgtcaaa gtgaaccaag tcgaactggc agtgatcaac atgcaggctc
     4681 gttcttttat ggtggacttg gatgatcctt ttcgtaaaat gaatgagctg gtagaagaag
     4741 ccagaaaacg tacgccgatc atttttgtag attttcatgg agaaacaaca agtgaaaaac
     4801 aagcaatggg ctggttttta gatgggaaag tttcagcagt ggtcggtaca catacacacg
     4861 ttcagacaaa tgatgcgcga attttgccaa gaggaacagc gtatctgaca gacgttggga
     4921 tgacaggtcc ctatgatggt attttgggta tgcgcagaga acctgtaatc gaaaagtttc
     4981 tgactgcatt accaaaacgt tttgaagttg tggaacaagg ccgatcgatt ttaagcggct
     5041 gtatcttgga attggatgat acgacaggtc atgcaaaaga aatccagctg attcagatca
     5101 acgatgatcg accgttcatg gagtagaaag agaggcagtt ttttgcagca ggagccggaa
     5161 attcaaaaag aagtaaggaa actgacaaaa cttttaagag agaatgaaac gatcattcgc
     5221 tacaaagaac ttgaagaaaa gatccagcaa aaccagtatt tagctgagtt gagagaaaaa
     5281 atcaaacaag cgcaaaaaga tgctgttcat tttgcccact atgacaaacc agcagcagaa
     5341 aaagaagcaa tcaaacaagc ggatcaattc atgcaagaat ttgatcagca tccgcttgtt
     5401 gtagcttata gaaaacagct gttggaagca gatgatctgc ttcatcattt aacaactatg
     5461 atacaagaag aaatcaatgg acaaatagag gaggaaaaac atgcctcaaa aaactaaaaa
     5521 tactcctatg atggaacaat atctaagcat caaggcccaa taccaagatg cttttttgtt
     5581 ctatcgttta ggagactttt atgaactatt ttacgaagat gcgatcaaag cgtcacagct
     5641 tttggaatta acgttgacga gcagaaatcg aaatgcagaa gaacctattc ctatgtgcgg
     5701 tgtacctcac catgcagcac aaggatatat cgatgccttg attgaaaaag gctataaagt
     5761 agcgatttgc gagcaggtag aagatccaaa gacgactaaa gggatggtca agcgcgaagt
     5821 gatccagcta gtcactcctg gtacagtcat ggacagcaaa ggattgtcgg caaaagacaa
     5881 caatttttta acagctgtag ttagtcaagg aaatgaattt ggctttgctt atgccgattt
     5941 aagtacggga gaattgaaga cggctcggtt gcatgatgaa gaagctgttt taaatgaagc
     6001 ctctgcattg cagacaaaag aactcgtatt aggcagtgag atcactgata cccttcgtga
     6061 acagctaagc gggcgtttag ggctagtctt ttctcaacaa aatgagatgg aagaaaacgc
     6121 ggaatttagc tttttgacaa gcgaactagt cgatccgctg gaaaaagaag caaccggcaa
     6181 actattgact tatttatcgg tgacacaaaa gcggagttta gctcatatcc aaaaagcagt
     6241 agaataccaa ccagatcatt ttttgaaaat ggattattat tcaaaattca acctagaatt
     6301 aagccaatct attcggactg ggaaaaagca cggcactctt ctatggttgt tagatgaaac
     6361 aaaaacagca atgggtggcc gtttactaaa acaatggcta gaccgtccgc ttatccaggc
     6421 aaaacaaatt aaagcaagac aagaaatggt cgcttctttg ctagacagct attttgaacg
     6481 tatcgatttg cagtcagcat taacgaaagt ctatgatctg gaacgcctag caggtcgagt
     6541 agctttcggt aatgtcaatg ggcgagactt gatccagctg aaaacttctt tagaacaagt
     6601 gccaatgatc agagggttga ttgaaggaat cgatcaaggt caatggcaaa gtctgctttc
     6661 agaaatgcag ccgatggacc atctcgtcca attgatcgac caagctatcc aagatgatcc
     6721 gccgttacaa ataacggaag gaaacatcat caaagatgga ttcaatgagc agctcgatat
     6781 gtaccgttct gctatgagaa acggaaagaa atggttggca gaacttgaag caaaagagcg
     6841 tcaagaaaca ggaatcaaga acttaaaaat cggatttaac cgcgttttcg gttattatat
     6901 agaaattaca aaagcaaatc ttggaaatgc agaactggag aagtatgaac gaaaacaaac
     6961 tttagcaaat gcagagcgtt tcatcacacc agaactaaaa gaattggaga cacaaatcct
     7021 cgaagcagaa gaaaaatctg ttgatttaga atatcagttg tttttagctg tgagagaaga
     7081 agtcaaaaag gctatccagc cgctacaagt acttgcaaaa gcaatcagtg cagtagatgt
     7141 gctacaaagt tttgcaacga tcagcgaacg ctatcagtat gttcggccag aacttgtcag
     7201 tgacaagcat cagcttctga tcaaagacgg acgacatcct gtagtagaaa aagtattagg
     7261 acatcaggaa tatatcccta acagcgtaga aatggcagaa gacgaaatga tcttgctgat
     7321 tacaggaccg aatatgtctg gtaaaagtac gtatatgcga cagttggcat taactgttct
     7381 gatggcgcaa atgggctgtt tcgttccggc aaaacaagca gttttaccta tttttgatcg
     7441 gatttttacc cgaattggtg caagtgacga cttaattgct ggacaaagta cttttatggt
     7501 agagatgatg gaagccaatc aagccctgcg ttatgctacg ccaaacagtt tgattttgtt
     7561 tgacgaacta gggcgaggaa cagcaaccta tgacggaatg gcgttggccc aagcaatcat
     7621 cgaatatatc catcgtcatg tacaagcaaa aacactcttt tctactcatt atcatgaact
     7681 gacggtactt gagaaagaac ttccgcagct taaaaatgtc catgttggag ccgttgaaaa
     7741 agacggagaa gttgtcttct tgcataaaat gatggatgga cctgcggata aaagctacgg
     7801 catacatgtg gcaaagatcg caggattgcc ttccaatcta ctagaacgag cagcagatat
     7861 cctgcatacg ctagaaaatg gcgataacgg acaccatact gttccagctg aagtagaaga
     7921 gaaaactaaa acagccaaac aggagcaaat aaaaccacct gcagaaaaag taaatcaaga
     7981 ccatatacaa gaccgtgtaa aagaagaaaa ccaacagctt tctttattca gtgaattatc
     8041 cgaaaatgaa acagaagtca ttgaatcact gaagcaaata aatttactag aaatgacgcc
     8101 aatggatgca ctaaacacat tatatgaatt acaaaaacaa ctgtaggaag aaggcggata
     8161 tcgatggcca aaatccaaga attatctgaa cgtttagcta atcagatcgc tgcgggagaa
     8221 gtagtggagc gaccagcttc cgttgtgaaa gaattagtgg aaaatgcaat tgacgccggc
     8281 agtactcaga ttgatatctt agttgaagaa gcgggactgc ggacgatcca agtgattgat
     8341 aacggagaag gcattttggc agacgacgta gagaatgcct tcaagcggca tgcgacaagt
     8401 aagatccata atcgcgacga tctttttcgc atacgaacgc tgggctttcg aggggaagct
     8461 ttgccaagta ttgcctctgt ttctgaaatg actctggaaa cggcgaatca ggaagaaaaa
     8521 caaggcacct atctgtcgct gaaaggcgga gaagttattg agcatcgacc agcaccactg
     8581 cgtcaaggaa cgaagattac cgtcagcaat ctgtttttca atacgccagc ccgcttgaaa
     8641 tacgtgaaaa cactgcacac agaattagcg aacatcgggg acatcgtcaa tcgactggca
     8701 ttgagtcatc catcgattgc ttttcgttta gtacatgatg gaaacaaaat gctggcaact
     8761 gctggaaatg gcgatctgaa acaaacaatt gcgggaatct acggattatc taccgcaaaa
     8821 aaaatgctga aaatcgaagg aaaagatcta gattttgagt tatttggcta tgtttcattg
     8881 cctgaggtga ctcgtgccag taggaattac ttatcgacga tcatcaatgg acgttttatc
     8941 aagaactttt ccttgaataa agcaatcgtt gcaggttatg ggtcaaaatt aatggtcggt
     9001 cgtttcccta ttgctgtatt ggaaatcaaa atggacccgc ttttagtaga tgtaaatgtt
     9061 catccgacga agcaggaagt ccggctgtcg aaggaaaaag aactaacaga gctaattagt
     9121 aacgccattc aaaaagcatt gtctcaagaa aatctgattc caagtgcagc ggataatcta
     9181 cggtttaaga aaaaagtacc tgatcagcct aaaacagaac aaatcgaaat cgatttgtcc
     9241 tacgcttttg aagaagaaaa acctaaaaag ccaagtagtt tgaattttga tcgacagaca
     9301 ggtacctttt tcgtagaaga actaaagaac acatttgttg acagaattgt tgataagtct
     9361 gtggaaaatg ttgaaaacta ttctggtttt tctgaagaaa acaccactta tccagaaaaa
     9421 acagcgaaaa cggtggataa gtttggtgaa aatgttgata actttgtaga aaaaacagtg
     9481 gaaaaatctg aaaagaaaga atttcccatt gtggataaca tggagaaaac aaatgaagaa
     9541 caagcagata aatccgtaga agaggatgaa aagatttacg aggaagaatt gacactccat
     9601 cctgaatttg atttcactca aggaaacgac aaaacattag aacaagccat caagaaatta
     9661 gaaacggaaa aaccagtaca acgttttcct cagttggaat attttggaca gatgcacgga
     9721 acgtatcttt ttgcccaaag tagcgatggg ttgtacatca ttgatcagca tgctgcccaa
     9781 gagcgcatta agtatgaata tttccgggaa aaaattgggg aagtatctga tgatctgcaa
     9841 gaattgcttg ttccatttgt gttagactat ccaaacagtg atgctttgaa attaaaagag
     9901 caaaaacact tgctagaaga tgcagggatt tatttagaag aatttggtca aaacagcttt
     9961 attgtacggg cccatcctac ttggtatccc aatggagaag aagaatcgat tattcgggag
    10021 atgatcgata tgctgctgac aactggaagt gtgagtgtaa aaaaattccg tgaagcaaca
    10081 gcaatcatga tgagttgcaa acgatcgatt aaagccaacc attatttgaa tgaagcacaa
    10141 gcacgtgtgc tgctgaagga tctggcacga tgcgaaaatc ctttcaattg tccccatggc
    10201 cgtccagtat tgattcactt taccaattcg gatatggaaa ggatgttcaa acgaatccaa
    10261 gacccgcatt aagtcaaaga gaagagggaa aacatgaaga ttgtattagc atcccaatca
    10321 ccaagaagaa aagagttatt ggctcatatc gtttcagaat tcgagattga acctgctgat
    10381 atcgatgaaa cgccatttcc agacgaacag cccgtcgatt atgttcgtag aatggctgaa
    10441 gaaaaagcac gaactgtctg ggaacaagat gaaaaagaag accatttagt gattgccagt
    10501 gacacgactg ttgttctcaa tcaagagatc atgggtaaac cagaagatct ggctgaggct
    10561 gaattcatgc tgaaaaaact aagcggggaa acacatactg tatatacagc tgttgtttta
    10621 aaaacaaagg aacgagaaga acggatacta gcagaagcac atgtcacttt ttatcctttg
    10681 acagacgaag agatcaaacg ctacttggag acgggggatt atgcggataa ggcaggggca
    10741 tacggcatcc aaaatgctgc gagtgtattc gtcaaagaaa tttctggaga ttattacagt
    10801 atcgtaggat ttccgattgg tgctgtcaat caggcactga aaacattcat ttaaacaaaa
    10861 aagcggacta tctgattttc gattgaaaat ccatgatagc ccgttttttt tattgaaaag
    10921 attacttatc tactctgact aagattttac ttcagcttta ccgactgctt cgcccgggcg
    10981 aacaagtcct gattggtcaa gagaaagtcc tgcaactttt tcattattgg tcaaagcaac
    11041 aataatgtca gttttcttgc cagcttgttc tatttctggc aaatccattt cagcaatcag
    11101 ggtctctggt gtgacaaggt atccttcttt cacgaataca ttgaaaggaa caccctttag
    11161 ttcaacagta tctaatccca tgtgaacaag tacttccagc ccctcattag aaagaatacc
    11221 aatggcatgt tttgtttcaa agacactcgt cactttaccg tttactggtg catagacttt
    11281 gccgtttgac ggttcgacag cataaccgtc acccatcatt ttttgtgaga aaactgggtc
    11341 atttacttca gttatttcct tgatggtccc tgcagcgact gcgaataatg tttcttttgt
    11401 tgcaactgcc gtaccagttg ctgaaacagg ttcgaaagac tgtggcgttg ctgttgcgcc
    11461 atttgctgtt tgaagttgta catcagcagc attttcagga acagcaggat caatcttatt
    11521 caaaattccc tgacgtctaa agatgatcgt caagccaaat ggaatgatga cagcaatgat
    11581 cattgcgatg atgaaaggta cccatgtttc tgcttggata gctagtattc ctggcaaacc
    11641 gcctactccg atcgcgttag ctcgaacacc catcaagtta gcaaacatgc cagctaatcc
    11701 agagccgacc attgcagcga cgaaaggata gacgtatttc aaattgattc cgaacatagc
    11761 aggttcagtt acgcctaagt aacaagaaat cattgctgga atcgagattt gttcttcttt
    11821 tttattccca cgatgtaaga agatgatggc taagactgca gagccttggg cgatatttga
    11881 taaagcaatc atcggccata aattcgttga tccgaaatct gcaatcagct gcatatcgat
    11941 ggcattggtc atatggtgga gaccagtaat cactaaagga gcatacagga atccgaatac
    12001 ggcactaaac aaccagctga tagaagacgt caatccagta tttacgatat ttgagatcca
    12061 gcttccgact gtccagccga taggacctaa aataacgtgt gcagctaaaa cagttggtaa
    12121 caatgaaaac aatggaacaa aaatcatcga gatcgactga gggatatatt tgcggaagaa
    12181 gatttctaga tacgctaaca taaaccctgc cagcatggct ggaataactt gcgcttgata
    12241 accaatcatc tggacttgag caaaaccaaa atcccaaaat ggaatatctg ctgcggctgt
    12301 ggaagctaca ctataagcat ttaataattg cggagaaact aacgtaattc ctaaaacgat
    12361 tcctaatatt tgtgtagtac ccatcttttt tgcaatactc caagtgatac ctactggaag
    12421 aaaatggaaa attgcttcac cgggtaacca caaaaaggca ttgacaccat tccaaaactt
    12481 cgagtgttca acgattgttc caccaagact ttcaaactgg ataccttcaa gtacatttct
    12541 gaatccaagg atcaatccac cgacaatgat tgcaggaatc agtggtgtaa agatttctgc
    12601 taaaacggca atagcacgtt gaacgggatg aaggttttgt tttgcagctg cttttccttg
    12661 ttctttggaa accccttcaa ctccagatac tgcaacgaag tcgttataaa aagtagaaac
    12721 atcgtttcct atgatgactt ggaactgtcc tgcattcgtg aacatccctt ttacgctagg
    12781 tatatcttcg atcgcttttt cgtccgcttt tcctggatca ttcaggacaa aacgcatacg
    12841 agtcgcacaa tgactaactg cagcaatatt ttcttttccg ccgatatcat gtaacagctt
    12901 ctcggcatct gcttgatatt ttgccatatc aataaacccc tttctcttaa cttgtctatg
    12961 caagttgttt cttcatttac tatacttttt ttcggagaga attgcaagcg ttttattaaa
    13021 aagtacgaga gaaaatgata tcgttttaaa aagcttaaaa taagcttttc ttaaaaatag
    13081 gaacattgaa atacaaaaag agctgtagta tagtgaagaa atgatggaag aacgagtgag
    13141 ggaaaaaaca tgaataagtt caatgaaatt tttttagatt tggaacagaa aattttagcg
    13201 aaagaatatc ctccgcattc gcttttaccc agtgagaacc aattgatcaa aatttatggt
    13261 gtttcacgag aaacaattag aaaagcgctg aatcttttga ctacggaagg ttatattcaa
    13321 aaaaaacaag gaaaaggttc gatcgtactg gatcggaatc gcttcgactt tcctatttct
    13381 ggattgacta gctataaaga gctacaggag acccaacgta ttccaagtga aacgatcatc
    13441 catacgttag aagaaacaga agtgacaaaa gcattgaacg agatcactgg ctgggagatc
    13501 ggtgcgcctg tttggcattt gatacgcgaa agaaaaatca atggcgaagt agttatatta
    13561 gacaccgatt atctattaaa ggaaatcgtt cctattttaa ctccagcaca agcacaagga
    13621 tcgatctatg aatattttga aaatgagctt tctttgacga ttgcttacgc gcaaaaggag
    13681 atcacagtag aagaagtaac ggaaatcatg aaaacagcga tggatcttca cgatgacact
    13741 tatgcagtag tagtcagaag cctcgttcat ctggaagata cccgctgttt cgagtattct
    13801 gagtcggttc accgattaga taaattccgt ttcgttgagt ttgcccgacg ccgaaaagtg
    13861 taaatagttc aaaagaaagc aaattgttgg catctgtcca ctttggcgct atgataggat
    13921 cacaaagggg gcatttttaa tggataaacc aataagacca gaaaaagaag aactgaaaga
    13981 aaagttatca cccatggctt atgcagtcac acaagaaaat gcaacagaac gaccatttag
    14041 cgggaaatat gatgattttt acgaaaaggg gatctatgta gatatcgtca gcggtgaacc
    14101 gttattcagc tctgcagaaa aatatgatgc aggttgtggc tggccatcat tcagcaaacc
    14161 aataacgaaa agacaagtgc gcgaaaaagc cgatttttct catggcatgc atcgaataga
    14221 agttcgaagc aaagaagcag attctcatct cggacatgtg tttaccgatg gaccagtaga
    14281 tcaaggtggt ctacgttact gcatcaattc tgcctcactg aaattcattc catatgaaaa
    14341 gatggatgaa ttaggttatg gggaattcaa atcacttgta gaataatgga aatacacaaa
    14401 gagcggtaca atacggggat ttactcgatc gtccgctctt tttttattca agcgattgct
    14461 atttgaaaga aacgctccct agactccttt tttcacaagg tgaaatgtgc tacaataaag
    14521 ggaacgtatg tgtgcaaaag aaagaggact agaatatgta tgaatatctg acaggtgtgg
    14581 ttacctttat caacccctat tacatagtag tagaaaccaa tggtatcggc taccagatcg
    14641 cactaggaaa tccttaccgc tacagtagca aactaaatca aaaaataaaa atttatgtcc
    14701 atcaggtaat acgtgaagat gcgcatcttc tgtatggatt tgattcgcta gaagaaaagc
    14761 aactgttttt gcgattagta agtgtttcag gaatcggtcc gaaaagtgcc cttgcaatca
    14821 tggcaagcga agaccactcc ggtttgattc aagcagtaga atccagtgat gtcacttatt
    14881 tgacgaaatt tccaggagta gggaaaaaga cagcacaaca aatgatcttg gatctaaaag
    14941 gaaaattcgg tgagctgagt atcgatacac cttttagtct atttgatgaa gcaaacacaa
    15001 aagacgctac agctctatct gaagcaatgg aagctctctc ggctcttggt tacagtgatc
    15061 gagaaatcaa acgtgtagaa aaacaattaa aagaagtaga taatcagaca acggatgagt
    15121 atctacgcca agcactcaag ctaatgatga aaaaataagg aggagaccaa atgacagatg
    15181 aatacttgtt atcaccagaa tctggcgaaa atgaactgag tttggaaaaa tctcttcgac
    15241 cacagctgct ttctcaatat atcggacaga gaaaagtcaa acaagaactt cagatttaca
    15301 tcgaagctgc cagaaatcgg gaagaacctt tagatcatgt tctgttgtac gggcctcctg
    15361 gattaggaaa aacgacgatg gccatggtca ttgctaatga gatgaccgta aatattcgta
    15421 cgacgagtgg ccctgctatc gaacgtccgg gtgacttagt agcaatcttg aatgaactgg
    15481 aagcagggga tgtcctgttt atcgatgaga tccatcgatt acctagagtg gtagaagaga
    15541 tgctttattc agctatggaa gatttctatg tagatatcat ggtaggacaa gggccaaccg
    15601 cacatccggt tcatttccct cttcctccct ttactttgat tggcgcgacg acacgggcag
    15661 gtatgctttc tgcaccttta cgagatcgat ttggcatcat ttctcatatg gaatattatg
    15721 acgaacagga tctgcaggag atcgttttac gttcttcgga tatttttcaa acagagatca
    15781 ttgaagaagg ggcattggaa attgctcgcc gatcacgagg aacgcctaga atcgctaatc
    15841 gcttgctcaa acgggttcgt gattttgcgc aagtccaagg agacgggaaa atcgatcgtc
    15901 ctatagcgga tcaggcactt actttgctac aggtcgatca ggctggattg gattatgttg
    15961 accagaaatt gttgcgaaca atgatcgagt tatatggcgg cggaccagta gggttaagta
    16021 cgctttcagt aaacatagga gaagagcgtg agacggtaga agatatgtat gaaccgtatc
    16081 tgatccaaaa aggattttta aagcgtacac ctagagggcg aattgctact gcttatgcct
    16141 atgaacattt tggttacgac tatcatatat gatttttgct tttagattgg aggaaaatga
    16201 atgagtgata caacacgtac gaaacaattg atcaaaaacg tattgattga tttgtgccaa
    16261 acgcaatctt ttcgaaaaat cagcgtccaa aaaatcgcaa acgaagcaaa aatcaatcga
    16321 caaacatttt attatcattt tacagataaa tacgatttgc ttcgttgggt ctattgtgag
    16381 gatgcattgc attatttaga tccacaatgt ctttcacttg ataattggga agagcaagca
    16441 ttgaaaatgc tgaaagcaat tgctgagaag aaagaatttt atgcaaatac tgtgctttct
    16501 gacccagaaa ttttacaaaa agagttttct caactgatcc aaaaatcatt tatcaagatt
    16561 tttgaacaaa tggatgaaga aagacaactt tctgcaagag acaaactatt ttatgctcgt
    16621 tttttttctt acggttgcag tggtgtatta gtcaattgga tcacgaatga atatgaagaa
    16681 acaccgatgg aaatcgctat gcagcttttc cgtttagcca aggatacgga attatattct
    16741 tatcgtcttt atgcaaaaga agagttggaa gaacagtaat agaaaaattt tttgttggaa
    16801 aagcaataat cgctaaaagg agatggggaa atgacaaaaa ttttatttgt ttgtttggga
    16861 aatatttgcc gttcaccgat ggcagaaggc ttgctgagaa aacgaatagc attagaagga
    16921 agggaagcag aattttttgt ggattctgct gctacaagca cgtatgaagt tggaaagaca
    16981 ccgcaccctg gaacgaaaaa aatattaaac caagaaaaag tagatatgac gaatatggtt
    17041 gctcggcaaa tcacacccca cgattttgaa acttttgatt ggatcattgg catggatcaa
    17101 gaaaatgtag aagaactgaa acgaagagca ccacgctcag cgcaggggaa aatccatttg
    17161 tttttaagta gcattcctgg aaaagaaaaa gaaaatgttc ccgacccata ctataccaat
    17221 aactttgatg agacatatca cttgatcaca gaaggcctga atcattggtg gaatatttgg
    17281 attgatgaaa ataaaataac aaacaatttg taaagttaac gctttctttt taaggcgatg
    17341 ttagagcatt tccttgtttt tttgtaaaat gttaggtaaa ataaaattaa ttaagtaaca
    17401 ctttcttagc taaaggagga gtgcattcga tgaacgaaga gacagtatcg ccaatgaaga
    17461 agcttacgaa gttagttact catacttcgt atcaactttt tgattctttt gtaaatgaca
    17521 ctgaagaaca tgctaagaat gtacgattgc aaggaaagtt tattcttgag ctgcaaaaag
    17581 ctgctactga acatagtcta gttatcttac aagtaagagc aaatcgtttg agtagcaagt
    17641 atgaaacgat ctcaggctgg gtggcgacaa aaacggtcga aaaagaccga gtgatgatcc
    17701 gcctacaaaa cgaagagcag cagatccgga tggttcctgt agaacaaatt gaaaaaataa
    17761 caatcctttc accagcaggg gaacaggaac ggttatcaaa ataatttata ccagatagaa
    17821 aagcacgtat gagcttgtac catcaatcat tactaatcaa aaataaccga atgctttaag
    17881 ctgatatcag taaaacggaa cgttttagga tgagtatcat agcatgacag cattcggttg
    17941 attagttgaa tcgattggat ggtacaagtt tttttgataa aaaaaggtcc tgctagccgg
    18001 ggagctagca ggataaggag ttaaaaatga aaaagtgttg ttagggttgt ttgttggtat
    18061 gcttatataa tagtcattag ttgtgaatat tttgtgacga aagttttcga aagttgttaa
    18121 gtt
//
LOCUS       DACGLW010000060        17833 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.062, whole genome shotgun sequence.
ACCESSION   DACGLW010000060 DACGLW010000000
VERSION     DACGLW010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17833
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.062"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            122..934
                     /locus_tag="IXT53_001999"
     CDS             122..934
                     /locus_tag="IXT53_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HAQ4905800.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            931..1731
                     /locus_tag="IXT53_002000"
     CDS             931..1731
                     /locus_tag="IXT53_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HAQ4905801.1"
                     /translation="MKQRVITAAVALVLFIPIIWIGGIAVELTAAILAVVGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            1892..3160
                     /gene="rseP"
                     /locus_tag="IXT53_002001"
     CDS             1892..3160
                     /gene="rseP"
                     /locus_tag="IXT53_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HAQ4905802.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAIVLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPGKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            3228..4937
                     /locus_tag="IXT53_002002"
     CDS             3228..4937
                     /locus_tag="IXT53_002002"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HAQ4905803.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPKRDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTSLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELANTLPILLN
                     QGE"
     gene            5149..9501
                     /locus_tag="IXT53_002003"
     CDS             5149..9501
                     /locus_tag="IXT53_002003"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HAQ4905804.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIQ
                     LGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSFIV
                     KKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYAPE
                     GEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGKKA
                     GVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAVKM
                     YKGNVIESFDEFIDPGHPLSRTTVDLTGITDEMVRGSKSEEEVLRMFLEFSKDTILVA
                     HNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSLEQ
                     HHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILAKN
                     QAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKGVE
                     EARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVATGN
                     VHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEIVV
                     ENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRLEK
                     ELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAPHY
                     YCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPDID
                     LNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSAEV
                     DRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHSIH
                     DNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIYSK
                     TGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDATLA
                     EVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVPDW
                     YIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMCKG
                     KDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFVIE
                     GDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGVLK
                     DLPDENQLSLFDML"
     gene            9645..10118
                     /gene="rimP"
                     /locus_tag="IXT53_002004"
     CDS             9645..10118
                     /gene="rimP"
                     /locus_tag="IXT53_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HAQ4905805.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            10142..11317
                     /gene="nusA"
                     /locus_tag="IXT53_002005"
     CDS             10142..11317
                     /gene="nusA"
                     /locus_tag="IXT53_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HAQ4905806.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            11339..11632
                     /locus_tag="IXT53_002006"
     CDS             11339..11632
                     /locus_tag="IXT53_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HAQ4905807.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            11629..11940
                     /locus_tag="IXT53_002007"
     CDS             11629..11940
                     /locus_tag="IXT53_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HAQ4905808.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            11953..14259
                     /gene="infB"
                     /locus_tag="IXT53_002008"
     CDS             11953..14259
                     /gene="infB"
                     /locus_tag="IXT53_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HAQ4905809.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSSDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            14286..14633
                     /gene="rbfA"
                     /locus_tag="IXT53_002009"
     CDS             14286..14633
                     /gene="rbfA"
                     /locus_tag="IXT53_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HAQ4905810.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKKAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(14741..14962)
                     /locus_tag="IXT53_002010"
     CDS             complement(14741..14962)
                     /locus_tag="IXT53_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ4905811.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYYRKKKVEFFTFLFLFFG"
     gene            15410..17773
                     /locus_tag="IXT53_002011"
     CDS             15410..17773
                     /locus_tag="IXT53_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HAQ4905812.1"
                     /translation="MDYSSKEYFDKMTAWWRAANYLSVGQLYLKDNPLLRRTLKPEDV
                     KKHPIGHWGTIPGQNFIYVHLNRVINKYDLNMFYIEGPGHGGQVMVSNAYLDGSYTEI
                     YPEVTEDETGMQKLFKRFSFPGGIASHAAPETPGSIHEGGELGYSLSHAVGAVLDNPE
                     VISAVVIGDGEAETGPLAGSWFSNVFINPVIDGAVLPILHLNGAKIANPTILARKSDG
                     ELANYFNGLGWEPFFIEGNDPEKLNPVMAEKMDQAIEKIKSIQKEARLKTATDVVMPK
                     WPVLIVRTPKGWTGPKEWDGEPIEGTFRAHQVPIPVDQEHMDHADALLRWLKSYEPEK
                     LFDAQGRILEEIREIAPTGDQRMAKNPITNGGIDPRPLIMPDWKKYTLQFEKPGSVKA
                     EDMTELGKFVREIIEKNPENFRIFGPDETKSNRLNQVFKTTNRQWMEKIEPENDEWLS
                     PSGRVIDSQLSEHQDEGFLEGYVLTGRHGFFASYESFLRVVDSMLTQHFKWIRKSHDL
                     SWRKDYPSLNLIASSTVFQQDHNGYSHQDPGILTHLAEKKAEFIREYLPADANTLLAV
                     MDKAFRSSEKINLIISSKHPRAQFYSAEEAAVLVNEGLKIIDWASTAKEEEPELVIAA
                     AGTESNLEALAAVTLLLEEFPKLKIRFINVVDLLKLRHPSQDPRGLSDEEFDQYFTKD
                     KPILFAFHGYETLVRTIFFDRHNHHLMIHGYKENGDITTPFDMRVVNELDRYHLAKDA
                     ALKIKGSQAEDFAEKMDQKLQEHQNYIRENGIDLPEVLDWKWKNLDQ"
BASE COUNT         6095 a         3154 c         3920 g         4664 t
ORIGIN      
        1 taaaaaaaga aaaaattatt cagtttttaa ataaaaaaac aatgatttca taggaaaaac
       61 agttagatat tgctataata cttaagatag tgggaatctt ttaagtatag gaggaaaaag
      121 aatgttacgt ttttttccgc aaaaaaacaa atatattcaa gaaaaaagcg aatttcaatt
      181 taataccgat ggaccaattc ctcagcacat tgccatcatc atggacggca atggtcgttg
      241 ggcgcaaaac cgtcgtttgc cacgagttgc tggccataaa gaaggcatgg aaacagtaaa
      301 gaaagtcaca aaaaaagcat caaggcttgg agtgaaagtg ttgacgcttt atgccttttc
      361 gactgaaaac tggaagcgac caaaagatga agtaagtttt ctcatgcaac tacctgtcga
      421 cttctttgat acgttcgtac cagaattgat taaagaaaat gtcaaagttc atgtcatggg
      481 ttatgaaaac gtattgccag aacatacgca agatgctgta agacgtgcaa ttgagcaaac
      541 aaaaaacaat actggaatgg tattgaattt tgctttgaat tatggaagta gagcagagat
      601 cgtgactgct gtccaagaaa ttgccgaaga agtagcaaaa ggagaaatac acgcagaaga
      661 aatcgatgat gaattgattg ctaagcattt aatgacaggt ttcttgccta aagagttaca
      721 agatcctgag ctgatgatcc gtacaagtgg agaagagcga attagtaatt ttctgttatg
      781 gcagatcgca tatagtgagc tgtattttac aaaagcgctt tggcctgatt ttgatggtgc
      841 gcatcttgaa gaagcaatcg cctcttatca aaatcgagac cgtcgattcg gcggtgtaaa
      901 aaaaacagaa gatgagaaag gggatcagtc atgaaacaac gtgtgatcac agcagctgtg
      961 gcgctagtct tgtttattcc gattatatgg atcggaggca tagcggttga gcttacagca
     1021 gcaatcttag cggtagtagg agtttacgag ctgttccgga tgaaaggatt gacactctta
     1081 agttttgaag gagttttatc cgccgttggt gcagtgttac ttgttttgcc aaaagaaaga
     1141 tggtttttct ttcttccaga gaatgtaagt acatttatgt tattttatct tatagttatg
     1201 attcttttag gaacatcagt tatttctaaa aatacgtata cgattgatga agcaggtttt
     1261 cctgtgcttg tcagcttgta tgtgggaata ggtttccaga attttgtcaa tgcacgaaca
     1321 gaaagcttgg ctgttttgat atttggtcta tttattgttt gggcaactga tatcggagca
     1381 tatatggttg gtcgacgtta tggtcagcat aagttaagtc ctgatatttc gcccaacaaa
     1441 acgattgaag gtgcgctcgg tggtatcggt agtgctgttg tagtcgcatt tctttattta
     1501 ttgctatacc cagcaaaaga actattcaat catggtatgt ttgtcatgct tctgatgacg
     1561 attatttttt cagcagtcgg acaatttggt gatttggtcg aatctgcaat caaacgtcat
     1621 tatgatgtga aagactcggg aaatatctta ccaggccatg gtggaatctt agaccgtttc
     1681 gacagtttgc tgtttgtatt tcctatcatg catttatttg gcattattta gtaagtatac
     1741 ctgcgtttct ttgtgaattt cataaaagac gcaggttttt atttttcatt atattgatac
     1801 tattggtgtc tttggtatac tatggtaaaa ttatagtgtt ttatcgttgt tttataaaaa
     1861 acacaaaaaa caagtaaagg agaaaaaagc tatgaaaacg attctgacat ttatcatcgt
     1921 ttttggtata ttagtgattg ttcatgagtt tggtcatttc ttctttgcga aacgatcagg
     1981 aatcctcgtc cgcgaatttg ctatcggtat gggtccgaaa atttatgggc atcaagcaaa
     2041 agacgggaca acatatacgc ttcgcctatt acctattggc ggttatgttc gaatggcagg
     2101 caatggggat gatgaaacag aaatggcacc aggaatgcct ctttctcttt tactaaattc
     2161 tgacggcatc gtagagaaga taaatttaag taagaaaatc caattgacaa atgctatccc
     2221 gatggaattg agccgttatg atttagaaga tgagctgact atcactggtt atgtgaatgg
     2281 agacgaaaca gaagtagtaa cctaccctgt tgatcacgat gcgacgatta ttgaaaacga
     2341 cggaacagaa atcagaattg cgccaaaaga cgtacagttc caatcggcta aattgtggca
     2401 gcgtatgctg accaactttg ctggacccat gaataacttt attttggcaa tcgtgttatt
     2461 catcattctg gcatttatgc aaggtggtgt tcaagtgacg aacactaatc gcgtaggaga
     2521 gatcatgcct aatggagctg cggcagaagc aggcttaaaa gaaaatgatg aggtagtcag
     2581 tgtggacgga aaagaaatcc attcatggaa tgatctgaca acagtcatta cgaaaaatcc
     2641 aggtaaaaca ttagacttta agatcgaacg agaaggtcag gtgcaatctg tcgacgtgac
     2701 accgaaatct gtagagtcaa atggcgaaaa agttggccaa cttggtataa aagccccaat
     2761 gaatacaggt tttatggata agatcattgg agggacgcgt caagctttca gcggttcatt
     2821 ggaaattttc aaagccttag gatcactatt tacaggtttc agtttagata aattaggcgg
     2881 acctgtcatg atgtaccaat tgtcttcaga agcagcaaat caaggtgtaa caactgttat
     2941 tagtttgatg gcgcttcttt caatgaatct cggaatcgtc aatctgcttc cgattcctgc
     3001 cttagatggc gggaaattag tcttgaatat ttttgaaggg attcgcggga aaccattaag
     3061 tcaagaaaaa gaaggaatat tgacattagc tggctttgga tttttaatgt tgttgatggt
     3121 gctagttaca tggaacgata ttcaacgatt ctttttctag aaaaagttga caaatagggt
     3181 ataataacaa acgataacta ttttgaggaa ataaaggagt atatagaatg agacaatcaa
     3241 aaatgttgat tccaacatta cgcgaagttc caaatgatgc agaagtatta agccatcaaa
     3301 tcttgctaag agctggatat atccgccaag tatcagcggg tatttattca tatctgccat
     3361 tagcaaatcg tgtattggaa aaactgaaac gcattatgcg ggaagaattt gaaaaaatcg
     3421 gagcggtaga aatgctgatg cctgccatct tgccagcaga actttgggaa gaatccgggc
     3481 gttacgaaac atatggacca aacttgtatc gtttcaaaga ccgcaatgat cgaaaaatga
     3541 tccttggacc aactcacgaa gaaactttta ctgatttgat gcgtaatgaa atcaattcat
     3601 ataagaaatt acctttaaat ctttatcaaa tccaacctaa atatcgtgac gaaaaacgac
     3661 ctcgcttcgg tttgcttcgc ggaagagagt ttatcatgaa agatgcatat tctttccatg
     3721 caacggaaga aagtttggac gagacatata aagattacga aaaagcctat accgaagtat
     3781 tcaagcgttg tggcttagaa tttcgctcga tcatcggaga cggcggcgca atgggcggga
     3841 aagactcaaa ggaattcatg gcgatctcag aaatcgggga ggatacgatc tgttattcga
     3901 ctgaaagtga ttatgctgca aacttggaga tggctacaag ctactatgta tcaaaaaaat
     3961 cgcatgaaac acagcttgaa ttggagaaag tagctacacc taacgtaaaa tcaattgacg
     4021 aagtagcaga tttcttttct gttgaacctc aaaaaatcat caagtctgta ttgttcattg
     4081 cggatgaaca acctgtcctt gttttggtac gtggagacca tgaagtcaat gacgtaaaac
     4141 tgaaaaactt tttatctgct gactttttag aagaagcaac agaagaagaa gcacaaaaat
     4201 atctaggtgc tgagtttggt tctgtcggac cagtcggtgt ttcagatgac gtaaaagtat
     4261 atgcagaccg ttatgtccaa gatttagcga atgccattac tggagcgaac gaaacaggat
     4321 tccactataa aaatgtgaat ccaaaacgcg atttcactgt gctttcttat gaagatcttc
     4381 gttttgtaca agaaggagat ccttcaccag acaacaacgg tgttcttgcc tttactcgag
     4441 gaatcgaaat cggacatata ttcaaaatcg gcactcgtta cagtgaatct atgggagcaa
     4501 ctgtactaga cgaaaatgga cgagaaaaat ttgtgattat gggctgttat ggtatcggtg
     4561 taagtcgttt attatcagca atcgtagaac aacattcaga tgaacaagga atcaattggc
     4621 caaaaggaat tgcgccattt gatctgcata tcgtccaaat gaatctaaaa gacgaatacc
     4681 aaacaagcct gacgaatgaa ctagaagaag cgatgacaaa agctggctat gaagtactgg
     4741 ttgatgacag aaatgaacgc gcaggagtga aattcgcgga ttctgatttg attggctgtc
     4801 caatcagaat cactgtcggt aaaaaagcag tggatggaat cgtagaagtg aaaatcaaga
     4861 aaactggcga aatggttgaa gtacgtaaag aagaattggc gaatacttta ccaatcctct
     4921 tgaaccaagg cgaataataa gcgttaaaac acagaaaata aaaatagctg aatcagctgt
     4981 ttttgacaat ctgatgattc gcttttaaaa ttggaactgc gatttgaatc gcagttccaa
     5041 ttttttaatg taaaactcag aaaaaacgag tatactaaaa ggtactgaaa aagaagagga
     5101 taagatgcga tcaattgcat tgtttctcat cagaaagggc gtatatcttt gtcagaaaaa
     5161 cgagatttat ttgaaaaact gttggaacaa atccaactag aggaagcaga aaaaaatcat
     5221 ccactgctta ctgctggtga aatggatagt gtagtggtcc atcgtaaatc gcgtttatgg
     5281 gaatttacgc tacatttttc caaaattttg cctattatgc tatatcggtc attgactcag
     5341 catctgacta tcgcttttaa agatattgca caagtcaaac tgaatatctt agcagatgac
     5401 cagacattcg atgaacagct cttgcaagat tactgggccc aagctttgga aaatcagcaa
     5461 tgcgatacac cactagcaca gcaagtattg aaaacgcagg tacctgtgat aaaagatcgt
     5521 aaagtcatct tgccaatcga cagtacagga gctatttctt atttgaagca acaatacttg
     5581 ccattagtag aagagttgtt cgtcagttac ggattcccaa aatttcgtat cgaaccagaa
     5641 gtggatgagc agcaagcgga acgtgtattg aaactttttg aagaacgcaa acaagaacag
     5701 gcagaagcct ttatgaagca agcagctgaa agtctagttg tccacgaaca aaagaaaaag
     5761 gaaagaaaag agcaatcgcc agctcttgaa ggacccatcc agctaggacg aaatattcca
     5821 gccgatgaac caacgacacc aatgatcagt atcgttgaag aagaacgaag agtcacactt
     5881 gaggggtacg tattcgacaa agaagtcaga gaactacgtt caaaacgcaa gatcttgact
     5941 ttgaaaatca cagattatac ttcatcattt atcgtcaaaa aattctcgaa cggcgaaaaa
     6001 gatgaacaag tatttgatgc gatcagtgtc ggtagctggt taaaggtacg aggaagtatt
     6061 caagaagata cgtttgtccg tgatttggtc atgaatgcgc aagatatcat agaagtcaaa
     6121 catacacctc gcaaagatta tgctcctgaa ggggaaaaac gagtagagct tcatgttcat
     6181 agcaatatga gtacgatgga tgcaacaaac agcatctctg atcttgtggc acaagcagga
     6241 aaatggggac accgagctat cgccattaca gaccatggtg gtgcgcaagc ctttccggaa
     6301 gcacatagtg ctggaaaaaa agcaggtgtg aaaatcttat acggcgtgga agcgaatgtc
     6361 gtagatgatg gtgtcccaat tgcttataat gatgcacatg aagcattgag tgaagccaca
     6421 tacgtcgtat ttgacgtgga aacgacagga ttgtctgcag tatatgacac gatcatcgag
     6481 ctagccgcag tcaaaatgta taaaggaaat gtgatcgaaa gctttgatga atttatcgat
     6541 ccaggacatc cattgtcacg aacaacggta gatctgactg gtatcacgga tgaaatggtt
     6601 cgagggtcaa agtcggaaga agaagtattg cggatgttcc ttgaattttc aaaagataca
     6661 attttggtag cccacaacgc ggcttttgat atggggttct tgaatactag ctacgcaagg
     6721 tatggtattc ctgaagcggc caatccagtc attgatacgt tggaattagc tagatatctg
     6781 tatcctcaat tcaaacgttt cggattaggt gtgttatcta aaaagtttgg tgttagcttg
     6841 gaacagcatc accgagcaat ctatgatgct gaagcaactg gtcacttagc atggatcttt
     6901 gtgaaagagg caatggacaa tcataacatg ctttatcatg atcagctaaa tgagcatatc
     6961 ggagaaggag attcttacaa aagggctcga cctttccatg taacgatttt agcaaaaaat
     7021 caggcaggac tgaaagattt gttcaaactg atttcaatgt cgaatgtcga atacttcgaa
     7081 cgcgtcccac gaataccacg ctcccaattg aaaaagatgc gtgaaaatct attgatcggc
     7141 tcagcttgtg ataagggaga aatattcgaa gccatgatgc aaaaaggggt agaagaggca
     7201 agaaaccgag caaaattcta tgattatatt gaagttatgc caaaagcggt ctatgcgcct
     7261 ttgatcgagc aagaattggt aaaaaatgaa catgatttgg aagaaattat ccaaaatttg
     7321 gtagaaatcg gaaaaagttt ggataaaatc gttgttgcaa caggtaatgt acattatctg
     7381 aacgaagaag atgccattta ccgaaaaatt ttgatcaatt ctatgggcgg agcgaatccg
     7441 ttgaatcgtc atagtttgcc ggatgtccat ttccgaacaa ccgatgagat gctaactgca
     7501 tttcactttt taggggaaga aacagcgaaa gagatcgtag tagaaaatac gaataaaatc
     7561 gcagatatct gcgaagaagt gattccagtc aaagatgaac tttatacacc gaagattcca
     7621 ggttcagaag atgaaatatc agaattgagt tacacaaaag caaaacaaat gtacggagat
     7681 ccgttacctg agatcattca aaaacgatta gaaaaagagt tgaattctat taatggtaat
     7741 ggcttctcgg ttatttatct gatcgcccaa aaactagtgc ataaaagtaa tgaagatggc
     7801 tatctagtag gttcacgtgg ctctgttggc tctagctttg ttgcgacgat gacaggaatc
     7861 actgaagtta atccattagc ccctcattat tattgtccag agtgccaata ttccgagttt
     7921 tttgaagatg gtacgtatgg ttctggattt gacatgccag aaaaacaatg tccaaaatgt
     7981 ggtgcacgct taaacaaaga cggtcatgat attccatttg agacattttt aggattccat
     8041 ggagacaaag tgcccgatat tgatttgaac ttctcaggtg attatcaagc agaagcccat
     8101 aactatacga aagtattgtt tggcgaagac tatgtttacc gtgccggaac gattggtaca
     8161 gtcgccgaca aaacagcata tggctatgta aaaggatacg aaagggacaa taatctgcag
     8221 tttcgaagtg cagaagtcga cagattagca aaaggcgcta ctggagtcaa acggacaacc
     8281 ggccagcatc caggggggat catcgttatt cccgattata tggatgtcta tgattttacg
     8341 ccgatccaat atccagcaga cgatcaaaat tcagaatgga aaacgactca ttttgatttc
     8401 cactcgattc acgataatgt attgaaatta gatattctag ggcatgatga cccaactgtt
     8461 attcggatgc ttcaagattt atcagggatc gacccacaaa cgatccctac ggatgacccg
     8521 gaagtgatgc ggatatttgc cggtcctgaa gtacttggag taagtcagga acaaatttat
     8581 tccaaaacag ggacattagg tattccagaa ttcggcactc gttttgttcg agggatgtta
     8641 gaagaaacgc atcctacaac ttttgcagaa ctactgcaaa tttccggact atctcatggt
     8701 acagacgtat ggttaggaaa tgcggaagaa ttgattcgac gaggagatgc gacactggcg
     8761 gaagtaatcg gctgtcgtga tgatattatg gtttatttga tccatgctgg attagatagc
     8821 gggatggcat ttaaaatcat ggaaactgtt cgtaaaggac aatggaacaa gatccctgat
     8881 gaattgcgag aaacctatct atcggcgatg aaagaaaata atgtgcccga ctggtatatc
     8941 gattcttgtt caaaaatcaa atatatgttt ccgaaagccc atgctgcagc ttacgtattg
     9001 atggctcttc gtgtcgctta tttcaaggtt tatttcccta ttttgtatta ctgtgcatac
     9061 ttttctgtac gtgcagacga ctttgatcta gtctccatgt gcaaaggaaa agatgctgtg
     9121 aaacaagcaa tgaaagaaat cacggacaag ggattagatg cttccgtaaa agaaaagaat
     9181 caactgacag tgctggagtt agccaatgaa atgctggaac gaggattcaa atttggaatg
     9241 attgatttgt ataaatcgga tgccgttaac tttgttattg aaggtgatac gttgatcgca
     9301 ccatttagag ctgtcccaag tttgggaacc aacgtggcga aacaaatcgt tgaagcaaga
     9361 aaagacggtc catttttatc caaagaagat cttgccaccc gcggtaaagt ttcaaaaaca
     9421 ttgatcgaat atatgaacga caatggcgta ttgaaagatc taccagatga aaatcaatta
     9481 tcgttattcg atatgctata agcatctgtt ttcctgtctg cttgtaattt taagcagagt
     9541 atggtatagt tcttataggt aatgatacta actgtgagtg agcggatttt tcgctcactc
     9601 tttttaatgg aattacgcac ttaattttta aggaggcgaa acttttgagt agcgtcgttg
     9661 aaacagtcac agaaatggtg acaccgattt tagatgaaca aaattttgaa cttgtcgaag
     9721 tcgaatttgt aaaagaagga aaaagctggt tcttacgagt atttattgac aaagaaggcg
     9781 gtatcgatat cgaagaatgc gcctttgtca gtgagaaact gagtgaaaaa cttgatgcag
     9841 cagatccaga tccgattcct caagcttatt tcctggaggt atcctccccg ggagcagagc
     9901 ggccattgaa aaaagaaagc gactatgaaa aggcacttgg tgattatgtc catgtctcct
     9961 tataccaacc agtagatggc gaaaaacaat atgaaggatt tcttcaatca tttgatgctg
    10021 aacagcttac attgaaaata cgcataaaaa cacgagaaaa agaattgact tttgaccgaa
    10081 aaaatattgc aaaagctcgc ttagctatcc aattttaatc acggagagga atcagaaaaa
    10141 aatgagtaaa gaaatgttaa atgcgttaga cgctttagaa gctgaaaaag ggatctcaaa
    10201 agaaatcgtg atcgatgctt tagaagcagc attggtttct gcatacaaac gtcactatgg
    10261 ccaagcacaa aacgtcgaag tggaatttga tcagaaaaaa ggaaagatcc atgtctatgc
    10321 ggtaaaagaa gtaacggaag aagtgatgga ttcacagttg gaagtctcat tgaaagatgc
    10381 acttttaatc aatccagcat atgagatcgg tgatacgatc cgttttgaag taacaccaaa
    10441 agattttgga cggatcgctg cgcaaacggc taaacaagtt attttacagc gtgtacgtga
    10501 agcagaaaga acgattattt ataacgaatt cagtgcttat gaaaaagaca tcatgcaagg
    10561 aatcgtcgaa cgccaggata aacgttatat ttatgtcaat cttggtaaaa ttgaggcagt
    10621 tctttctaaa caagatcaaa tgccaaatga attttatcaa ccacatgatc gaatcaaagt
    10681 ttatgtttcc cgagtagaaa atacgtctaa aggtccgcaa gtatttgtta gccgcagcca
    10741 tccagaccta ttgcgtcgct tatttgaaca agaagtacca gaagtctatg atggaatcgt
    10801 tgaaattgtc agcatcgccc gtgaagctgg cgatcgttca aaagtggcag ttcgctcaaa
    10861 tgatccaaat atcgatgcag tcggtacatg tgtcgggcca aagggacaac gagttcaagc
    10921 aatcgtcaat gaattaaaag gcgaaaacat ggatatcgtc gaatggaatg aagatccagc
    10981 tgtattcatc gcaaatgcat tgaatcctgc acaagtcatg gatgtgatct tcgatgagac
    11041 aaatccaaag gcctgcacag ttgtcgttcc agactaccaa ttatcgctag caatcggtaa
    11101 acgcggacaa aatgcacgtt tagctgctaa attgactaac cataaaatcg atatcaaatc
    11161 agaatctgac atgacggaat tttatgaaaa tcaagaacaa caaaaagaaa cagaagaact
    11221 gcatgatgaa gcaatcgttc agtcagattt aacagacgat gaatatgaaa caattgcatt
    11281 caacaatgaa acggtcgaag aaaaaccaga agcttaactt tagtaaggag gcaaagagat
    11341 gaaacaaagg aaaattcctt tacgcaaatc cgtggtttca ggtgaaatga aacctaaaaa
    11401 agagatggtc cgaatcaccc gttcaaaaga aggggaagta tcgatcgatc caactggaaa
    11461 aatgcctgga cgaggagcgt atgtctcact tgagccggaa gaagtacaac aagcttggga
    11521 taaaaaaatt ctcgatcgag tactcgaaac aacattaaca gatgagtttt atcaagaact
    11581 tcttgactat gtcactcacc aaaaagcacg taaagagtta tttggaaaat gaatacagtt
    11641 aatcggcaaa aagccatgaa tctcatcggt ctagcgatgc gcgcgggaaa actaatcacg
    11701 ggtgaagaac tgaccattgc tgaaatccgc aaacaaaaag caaaattggt gtttgtggca
    11761 accgatgcaa gtgagaatac aagaaaaaag ataaaagata agagttcata ttatgaagtt
    11821 ccttgctttg agctattttc tgaagcagaa atcacgcaga tgatcggaaa acctcgcaag
    11881 gtattcggaa taatggatgc cggatttgcg aagaagacca aggagctaat tgaaggttag
    11941 gaaggtgatt gcatgggcaa caaaagaatc tatgaattag caaaagaact caaaaaatct
    12001 agtaaagacg tcgtggaaaa agcacaacaa cttggcatcg atgtgaaaaa ccatatgggt
    12061 gcgattacta gcagtgatga gaagaaattg caacaagcgt tcaaaaaccc aagcacgaaa
    12121 caacaagctg cacaacaagc aagcagaaaa ccagccaatc aacagccgaa cgatcaacgt
    12181 aaaaaagaaa caacgaacaa tcaacaaaaa aataaaaata accgcagtta tcaagatcgc
    12241 ggtcaaggga gcggtcaagt gaatcaagga aaaaaaccaa caacaaacca aaataatacg
    12301 ggaaatacca gcagccaaaa tcgtcaagga acgagccaag gcggcaatca aaaccgtact
    12361 aacaataaca acaaaccaaa caataacaat cgcggcaaat ttaataataa taaccgtaat
    12421 cgttataata aaaaaggtaa aaaaggcaaa cagcaacaat caactaaacc agctgtacca
    12481 ccacgcaaat tccgtgaatt gccagatgta ttagagtata ctgaaggtat gaacgtagca
    12541 gatattgcga aaaaaattca tcgtgagcca gcagaaatca ttaaaaaatt atttatgctt
    12601 ggtgtcatgg tcaatcaaaa ccaagcactg gataaagata cgattgaact gttagcgacg
    12661 gactatggca tggaaccaca agaaaaagtc caagttgaca ttgctgatat cgataaattc
    12721 ttcgaggcag aagaagtcga tccagataaa ttagtctcac gtccgccagt agtaacgatt
    12781 atgggacacg tagaccacgg gaaaacaacc ttattggata cattgcgcca ttcacgtgta
    12841 acaagcggcg aagccggcgg aatcacacag catatcggtg cctaccagat cgatattgat
    12901 ggtaaaccaa tcactttctt ggatacacca ggacatgcgg cctttacaag tatgcgtgca
    12961 cgtggagcaa gtatcacaga tatcacgatc ttagttgttg ctgcagatga tggcgtcatg
    13021 ccacaaacag ttgaagcaat caaccatgcc aaagctgcag gcgttccaat catcgtcgct
    13081 gtaaataaaa ttgataaacc aggtgcaaat cctcagcatg ttatgcaaga attaagtgag
    13141 tatgaattaa ttcctgaagc atggggtgga gaaacgattt ttgttgagat ctcagcgaaa
    13201 ttcggccaaa acatcgaaga attgctggaa atgatcctgt tagtcgcaga agtagaagat
    13261 ttaaaagcag atccaacaca acgcgcaatc ggaacagtga tcgaagcacg tcttgataaa
    13321 ggtaaaggtc ctgtgtcaac cttacttgtc caagaaggaa cacttcacgt tggtgaccca
    13381 atcgttgtcg gtaatactta cggacgtgtc cgtgtaatga caaacgatct tggccgtcgc
    13441 gataaagaag caggacctgc aacgccagta gaaatcactg gcttgaatga tgtacctcaa
    13501 gcaggtgacc gtttcgtagt cttcgaagac gaaaagacag cacgtgcagc gggtgaagaa
    13561 cgcgcaaaac gtgcattatt ggaaaaccgt gctgccacaa gccgtgtgac tttggacaac
    13621 ttatttgaaa gcctgaaaga aggcgaattg aaagaagtta acgtaatcat caaagcagat
    13681 gtacaaggtt cagcagaagc actggctgca tcattgaaga agattgatgt ggaaggtgtc
    13741 cgtgtaaaaa tcgtccattc agctgtcgga gcaatcaatg aaagtgatgt aaccttagca
    13801 gcagccagca acgctattat catcggtttc aacgttcgtc ctacaccgca ggcaaaagtg
    13861 caagctgata ctgaagaagt cgatattcgt cttcaccgta tcatctataa agccatcgaa
    13921 gaaatcgaaa ctgcgatgaa agggatgcta gaccctgaat ttgaagaaaa aatcaccggt
    13981 caaatgatcg ttcgtgaaac cttcaaagtt tctaaagttg gtacgattgc aggtgcctat
    14041 gtaacagaag gatatattcg tagagatagc ggtgtccgtg taatccgtga cggaatcgta
    14101 atctatgaag gccaacttgc aagtctgaaa cgattcaaag atgacgtgaa agaagtcaaa
    14161 atgggttatg aatgtggtgc gatgattgaa aaattcaatg acatcaaagt agatgacgta
    14221 attgaaggat ttatcatgga agaagtcaaa aatgactaaa aactgataaa aggaggccaa
    14281 tcattatggc taactaccgt gatcgccgag tcggtcaaga aatcttaaaa gaagtcaatg
    14341 atattttgcg caaaaaggtc cgtgaccctc gtgtggagaa cgtaacgatc acagatgtga
    14401 aagttacggg ggacttacag caagcaacga tctattacag cattttatct gacttagcat
    14461 cagataagaa aaaagcacaa gatggattga ataaagcatc aggattgatt cgtcgagagt
    14521 tagggcacaa tctaagtatt tataagacac ctgaattgtt ctttgaactt gatgaatcag
    14581 ttgtctatgg gaaccatatc gatgaattgc ttcgcgactt gaacaaagac taatcaaaaa
    14641 atcccgcaaa tcctgtgaaa tcaggggttg cgggattttt ttgttttcat taagacgtat
    14701 tacctaagag ttccatgact ttagtctggt agcgtgtcag ctaaccaaaa aagagaaaaa
    14761 gaaaagtaaa aaattcgact ttcttttttc tatagtatat aaatttttgc atatagataa
    14821 agatcaagat accgataacg gaagctagat tgtaatctat tttagtgaca gtcaggtata
    14881 gccaagtaac tagaagatca gtggaacctg caccataaaa gtttgagttt gtcggtccgt
    14941 tgacagttaa aagaaaaatc acgttgaagt tattgatatt tccgattacg ccgcttcgat
    15001 ttggtcacga tccaaatttt ggataatccc tgtagaaatt agcatagtag ctgggatacc
    15061 aattcacata ttgacaaaga taacagagac ttttacataa aacggatcaa tcaggaaagg
    15121 aattgctcga tccagcccca gttttccaac aatgtgttaa ctggcctagc gtcattcagc
    15181 aaattatgca tgatgaggag cgaaatgaac tgcggaacag ccattgtgat aacgacaagt
    15241 tttttggatc aatgtaaaaa acgtcagcga aaggaaaaat ttcgtggttt tttatacctg
    15301 ttcatttggc tagatataaa atccaaacaa atgaacagaa gtgcttatta ggtttcaatt
    15361 gggagtatct gcgctagaat aagagtaata atgaaaagga gtgttatgta tggattattc
    15421 tagtaaagaa tactttgata aaatgacggc ttggtggcgt gctgctaatt atctttctgt
    15481 tgggcaactt tatcttaaag ataacccctt actgagacgt acactgaaac cagaagatgt
    15541 caaaaaacat ccaatcggtc attggggaac gattcctggc caaaatttta tttatgtcca
    15601 tttgaaccga gtcatcaata aatatgattt gaacatgttt tatatcgaag ggccaggaca
    15661 cggcggacaa gtcatggtat cgaatgctta tttagatgga agttacacag agatctatcc
    15721 agaagtaacg gaagatgaga caggaatgca aaaattattc aagcgatttt catttcctgg
    15781 aggcattgct tctcatgcag caccagaaac accaggttct atccatgaag gcggagagct
    15841 tggatactct ctttcacatg cagtcggagc tgtattagac aatcctgaag taatctctgc
    15901 tgtcgtgatt ggtgatggtg aagcagaaac cggtccttta gctggaagct ggttctcaaa
    15961 tgtctttatc aatccagtga tagatggagc tgttttaccg atccttcatt taaatggtgc
    16021 aaaaatagcc aacccaacga tcttggcacg taaatcagat ggagaattag caaattactt
    16081 taacggactt ggctgggagc ctttctttat tgaaggaaat gatccagaaa aattaaatcc
    16141 tgttatggct gaaaaaatgg atcaagcaat cgagaaaatc aaatccatcc aaaaagaagc
    16201 tcgcctgaag acagcaacag atgtcgtgat gccaaaatgg ccagtattga tcgtacgcac
    16261 accaaaagga tggaccggtc caaaagaatg ggacggggaa ccaattgaag gaaccttccg
    16321 agcacaccaa gtaccgattc ctgtagatca agaacatatg gatcatgcag atgctttact
    16381 ccgttggctg aaatcctatg aaccagaaaa attatttgat gcacaaggac gtattttaga
    16441 agaaatcaga gaaattgcgc ctactggtga ccaaagaatg gcaaaaaatc ctatcaccaa
    16501 tggtggaatc gatccaagac cgcttattat gccagattgg aaaaaatata ctttgcaatt
    16561 tgaaaaacca ggctccgtta aagcagaaga tatgaccgaa cttggtaaat tcgttcgcga
    16621 gatcattgag aaaaatccag aaaatttccg tatatttgga ccagatgaaa caaaatcaaa
    16681 ccgattgaat caagttttca aaacgacaaa tcgccaatgg atggaaaaaa tcgaaccaga
    16741 aaatgatgaa tggctttcgc catctggtcg cgtcatcgac agtcaattat cggaacacca
    16801 agatgaaggg ttcttagaag ggtacgttct gactggacgg catggttttt ttgcaagtta
    16861 cgaatcattc ttgcgagtgg ttgactctat gttgactcag cacttcaaat ggatacggaa
    16921 atcacatgac ctttcttggc gaaaagatta tccttcgctg aatctcattg caagctcaac
    16981 agtcttccag caagatcaca atggatatag ccaccaagat ccaggaatat tgacacattt
    17041 ggcagaaaag aaggcggagt tcatccgtga atatctgcct gcggatgcaa atacattgct
    17101 cgcagtcatg gataaagcat tccgctcgtc agaaaaaatc aatttgatca tctcttccaa
    17161 acatccgcgt gctcaatttt actcagcaga agaagcagcc gtcttagtga atgaaggctt
    17221 gaaaatcatt gattgggctt cgacagcaaa agaggaggaa cctgaattgg tcattgctgc
    17281 tgcaggaacg gaatctaact tagaagcatt agcagctgta actttgctgc tggaagaatt
    17341 cccaaaacta aaaattcggt tcatcaatgt cgtagattta ttgaaattac gtcatccaag
    17401 tcaagatccg cgtgggttga gcgatgaaga atttgatcaa tattttacaa aagacaaacc
    17461 gatcttattt gctttccacg gttatgaaac attggtccgc acgattttct ttgatcgtca
    17521 taaccatcat ttaatgattc atggctacaa agaaaatgga gatatcacga caccatttga
    17581 catgcgtgta gtaaatgaac ttgatcgtta tcatttggca aaagacgcag ctttaaaaat
    17641 caaaggaagt caagcagaag attttgcgga aaaaatggat cagaaattac aagaacatca
    17701 aaactatatt cgtgaaaatg gtattgatct gccagaagta ttggattgga aatggaaaaa
    17761 tcttgaccaa taaaatgata aaatcgatcc cgcaaaacag aattttttac attgtattat
    17821 ttgtcaaatt aag
//
LOCUS       DACGLW010000061        17656 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.063, whole genome shotgun sequence.
ACCESSION   DACGLW010000061 DACGLW010000000
VERSION     DACGLW010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17656
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.063"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            complement(<1..1778)
                     /locus_tag="IXT53_002012"
     CDS             complement(<1..1778)
                     /locus_tag="IXT53_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015508912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905813.1"
                     /translation="MKIIEKKIANKKINWLVVNFLVLIAMIIFTSNIAHASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSAVNTIVIGLMILSLMFVGYKMITGKGTVDLKSVGMNVVMSIALI
                     LLMPTMISSGIQFAKTFYKDSTTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNNLTRKNFQQTDLTQVLTDKVIDKLEKENPIADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNSGQGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSNGFNRAKGSGE
                     GEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERATKPFKSMRDI
                     ANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSVPTRLSDRKDGAKVEN
                     ADRIMSSSERMREAMKNNAESNNNPA"
     gene            complement(1765..1983)
                     /locus_tag="IXT53_002013"
     CDS             complement(1765..1983)
                     /locus_tag="IXT53_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905814.1"
                     /translation="MIKNRLIDQVWCLDQNTDSLELIETICFNTIVLDLRAENDNCVT
                     HVIINQKMAQKLSESLRKWAKGGNDENN"
     gene            complement(2059..3111)
                     /locus_tag="IXT53_002014"
     CDS             complement(2059..3111)
                     /locus_tag="IXT53_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAQ4905815.1"
                     /translation="MNEKEVESLVEKQGYSNSDIYAELYYQYPIFLNVNEKYKKMKLS
                     SKLVYMLLKDRNRLSLKNNLYDENQKIFLIFEIKNLVEISGMSKNTILSALDELQKVK
                     LLVKRKNGFNKSKMQMFPNFYYLLKPVLCKDDVYKINKITHQENRQKNLIVQNLNYGT
                     NQYSVDNSKFELCDTSMKTRDKNNFADSSKNELELYKSYKDSKDIKESHNDDLLESLS
                     TKNNKLDEELIETFIEENLLKQIWGESIVNCLKLASNSDYRQFDNWYKKFKYAIRSAE
                     KEQKIQLKIIYEICNNKYFVDHVREQLSMTLRDLIRRAKTDHRIKQKDDYIFASLKNK
                     ALELIELQISEGIDKQ"
     gene            complement(3264..3590)
                     /locus_tag="IXT53_002015"
     CDS             complement(3264..3590)
                     /locus_tag="IXT53_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905816.1"
                     /translation="MASWLIEEIENERRKIMDAGITVMLDKQQTNQLKNYVFEMTKEA
                     IDQARIDTGLERPFLKGKEMAKYLNVSYTTFLKFKRMGLPVILLEKMELFSKEECKKW
                     ILSHQI"
     gene            complement(3602..3802)
                     /locus_tag="IXT53_002016"
     CDS             complement(3602..3802)
                     /locus_tag="IXT53_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ4905817.1"
                     /translation="MITLNDQFIRSLRRHRADLILTKNDAAKLIGINRKTYVKIENGS
                     KESIRASTYQKLVNWLLNDLKI"
     gene            3877..4527
                     /locus_tag="IXT53_002017"
     CDS             3877..4527
                     /locus_tag="IXT53_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ4905818.1"
                     /translation="MSIKILLNLWFSCYNSLEFTIGGVTLNRIKFYIDSSKKTIKQIS
                     TETGINYTTLTNYYRGERNPRNTETWNILADYFHVPVSYLMGLDETNSIEQPNLDRIK
                     TARLEKKDKIVKNIASLDSDYFTELSNLYFSMTSLLEVKLQEDDSDFLFLKSILEDSF
                     EYTILFAKQKKFINKALNLNIDERKELESELFGNLTQVQVNNLKREITARLISKLQ"
     gene            4582..5748
                     /locus_tag="IXT53_002018"
     CDS             4582..5748
                     /locus_tag="IXT53_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ4905819.1"
                     /translation="MSIQKYTTKSGARYSVRLYVRKDENGKQEYYVKKGFKTEKEAKL
                     HEARKKVEIEDTGYHKPSNDLFITVYEEWLPSYRNRVQETTYQRTKDLFRLHILPKFG
                     NKSISKITPVHCQNAVNEWALKFKNIKQLKSYSSQIFEYAIFAELLHRNPMANTNLPK
                     REKKENENYFSLEELKAFWDILNKEEPLKHILIFQLLITTGIRKGELAALHWSDIYFE
                     EQLMYVRKSYATIRNEEKNARRKTVRIQKNTKNISSERILPIDAQTIEMLKIWKKEQA
                     RELLQFGINTNTKNQLIFTYVNADGDINQPLHADYSNNILKRLEKKYKFKHVTIHGFR
                     HTHATLLLEGGASIKETQDRLGHKNAETTLNTYSHVTEKAQRNALEKFIAYTGL"
     gene            6165..9392
                     /gene="fss3"
                     /locus_tag="IXT53_002019"
     CDS             6165..9392
                     /gene="fss3"
                     /locus_tag="IXT53_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HAQ4905820.1"
                     /translation="MYKKQKKKLCVLTILSLAVQSIVGAIAPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCVEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIADYQKKPSFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENTANIDYRVN
                     GNQLIISPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTVKIDVE
                     MEGSLKIKKVDKESGDIIPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTATIKAGETISVTSKNTREKGQIILDKTGIETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGHETQGKAEFKRAEYTL
                     FTVKDGKAVKWTENFKPELLKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDSEKNAVITRDVAAKEQVIRFGFDFFKFAGSTTGTAETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDYTKSDYIFTITEKGQKQPIKTVTVPYGNLTNENFSVSLNR
                     LMLYDLPEEEDSLTSLATWKEGDKELTTLDSTELVDKLSYNLHEIKEDWYIVAQAIDV
                     DATKAAQEKDKKAKPVAIAETTATLANKEKTGTWEILHKLTVEQVLDKTIVLFNYVYE
                     SKEAYEAGDEPVAKDASLNNQAQTVNCMVERHVSIQTKAHLEEGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALFPDGTSKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYDKDGNINGKHNEDLKEKSQTLTPKKVPTTPSTPEQSEMPTVP
                     SGSQESSPTVKAFPQTGEKTSNVLLFIGFSLIFATAGYYFWNRRN"
     gene            9504..9818
                     /locus_tag="IXT53_002020"
     CDS             9504..9818
                     /locus_tag="IXT53_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ4905821.1"
                     /translation="MELKFVVPDMVETFGKLTYGGEGEVLSEGYGRNTTIVGRSYHLF
                     SNKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLIAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            9831..10205
                     /locus_tag="IXT53_002021"
     CDS             9831..10205
                     /locus_tag="IXT53_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ4905822.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQLNQGKPTNQNEGKK"
     gene            10215..10559
                     /locus_tag="IXT53_002022"
     CDS             10215..10559
                     /locus_tag="IXT53_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HAQ4905823.1"
                     /translation="MEINVEHILDCLDQFGKGELTEEQLTNALTYDEKMFLIMHQGLL
                     EVGNDAKEDFDVLDWLGEEESFFMVVEVNESLCRQAESVLEEIDVEMPDAIERFLKQL
                     VETKQLPVVVND"
     gene            10628..11644
                     /locus_tag="IXT53_002023"
     CDS             10628..11644
                     /locus_tag="IXT53_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HAQ4905824.1"
                     /translation="MYKGHRIRAGDQHLIYHFVLGWLIFLFIGWMSVFYLQELRQFDI
                     SKVSFSTSEIGWSMKDVICLLGSLALSGSMMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQGEGFFKDLNSSWTKESISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LENKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIEEIVAENGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIQALLKSDAELFILDPKNADLADLGTIMPHVYSQK
                     EEISECVEDFYERMIARSRSMKEMPNYKTGENYAYLGLPPNFLIFDEYVAYMGANRFP
                     TSIE"
     gene            12238..14113
                     /gene="ltrA"
                     /locus_tag="IXT53_002024"
                     /pseudo
     CDS             12238..14113
                     /gene="ltrA"
                     /locus_tag="IXT53_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330681.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
     gene            14239..14595
                     /locus_tag="IXT53_002025"
     CDS             14239..14595
                     /locus_tag="IXT53_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HAQ4905825.1"
                     /translation="MILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRV
                     ALGRMSELGYSMMFGEVDKNFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREIS
                     NIVGLTVHTERENNSV"
     gene            14640..15782
                     /gene="dcm"
                     /locus_tag="IXT53_002026"
     CDS             14640..15782
                     /gene="dcm"
                     /locus_tag="IXT53_002026"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HAQ4905826.1"
                     /translation="MTFLDLFAGIGGFRLGMEQAGHQCIGFCEIDEFARRSYKAIHDT
                     RKEVEMHDITSVSDEFVRSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     ATLLQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQSRERI
                     FLIGHSRDACTEQVFPIIGSSSTSDQNIRNLVNINPSNRGMGGQVYGSDSVAPTLTGD
                     EGIKIALPVKDGISVAGMLSGNFEQGNRIYEVTGTAPTLSTKQGGTKIMIRKNETEYQ
                     EVKPGDSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKGVKAYDL"
     gene            15772..16061
                     /locus_tag="IXT53_002027"
                     /pseudo
     CDS             15772..16061
                     /locus_tag="IXT53_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395166.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            16317..17501
                     /locus_tag="IXT53_002028"
     CDS             16317..17501
                     /locus_tag="IXT53_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ4905827.1"
                     /translation="MAQRNLDYRLLKERRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     IASTKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDVRKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYYAIQDLL
                     KGRSIESTTFSIINRYLRFADKVDGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     IV"
     gene            17516..17611
                     /locus_tag="IXT53_002029"
     CDS             17516..17611
                     /locus_tag="IXT53_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HAQ4905828.1"
                     /translation="MEIVKDMLLVLGGSMLGVTVMCLMNASAAAD"
BASE COUNT         5880 a         3048 c         3415 g         5313 t
ORIGIN      
        1 cttgcaggat tattattcga ttcagcatta tttttcattg cttcacgcat acgttcagaa
       61 ctagacataa ttctatcggc attttcaact ttagcaccat cttttctatc tgaaagacgt
      121 gttggcacgg aatcagcata ttttccagtt tcatcttctt tatttttagc taacataggt
      181 ttgctatttt tattaatagc ggacactgta ccatcatcta aaccttcttt aaacttattc
      241 ttggtatcat tagctatatc acgcatactc ttaaatggtt ttgttgctct ttcactagcc
      301 attgtagctc catctgaagc taaaccggat aatccacgtt catgggcata tcccaaagct
      361 cttccagttt tgccagcttt ttttgataga gtttcgccga aattcttttc tggttttcga
      421 tcttctcctt ctccgcttcc tttagcacga ttaaatccat ttgatccttt tctaaacagc
      481 attgcgccca ttccaaaagc agaagcaagt tgcccatagc cttctttgag tccaatatca
      541 acaccaaagt atctcatgac tgttccactg cctttgatta aaacaaaaac ggcacagata
      601 taagcaattg tttttataaa aatatttgtt gagatacctt gtcctgagtt cagaaaattg
      661 ataaacatag caaacattga taacccaaat ccttgaaacc caacagttaa gtaacattgt
      721 agaatatctg atagaacaac ttttgaacgc tgtcctgtct caatatctgt tgcaaaaaca
      781 agaacaccta acacacgttt aaatgcaagt tctagtattg ctgtaatgat cacaaacgcg
      841 ctgaacacat aggcgattgc taacgctgtc aacccaattg aaatccccca tagatttgct
      901 tggtatcggt aatagcctga tttcaagcta tcagaaaatg ttgataagaa gttgtcagaa
      961 aactttgtag caacaaattc attattagaa ttttcaacta attcataacg aagattgtcc
     1021 gcaattggat tttctttttc caatttatca atcactttat ccgttaacac ttgtgtgaga
     1081 tccgtctgtt gaaaattttt tcgtgttaaa ttatttcgat cctccgtttt atcaatgctt
     1141 gagtactgat ctgttttatt aatatatgct aaatctgtta ctccttgttt tatcaatgac
     1201 caagcgactc cgtcatctga atttgtaatc gtagtagagt ccttataaaa tgttttagca
     1261 aattgaatac cactagaaat catggttggc attaaaagta ttaatgctat agacatcact
     1321 acgttcattc caaccgattt taaatccact gttccttttc cagtaatcat tttgtaacca
     1381 acaaacatca atgaaaggat cattaatcca atgacaattg tatttacggc tgattgataa
     1441 acattattta gccctgtact ttcaatgaaa ttgaacaaat ccagcacatc tggaatcata
     1501 ttttgaatcg aatctgtcac agtaaacatg ccttttacca cagcccatcc gagccaaatc
     1561 ccgaactgac taaggaaatc tccattgtac ttttttaggt aaggtctata aagagaatta
     1621 ttcaaaaact ctgtttgact ttttgtgtca tcaaaccaat tgaaaatact cgcatgagca
     1681 atattacttg taaaaataat catagctatc agaacaagaa aattaacaac caaccaattt
     1741 atttttttat tggcaatctt tttttcaatt attttcatcg ttacctccct tagcccattt
     1801 tctgaggctc tcagagagct tctgagccat tttttgatta attatgacat gagttacaca
     1861 attatcattt tcagctctta aatcgagaac tatagtgttg aagcaaatag tctcgattaa
     1921 ttcgagacta tctgtatttt gatctagaca ccaaacttga tctattaaac gatttttaat
     1981 caaaataacc cctcctatat tcaaagttct caaatgaagt ggagataccc ctccatttca
     2041 tttgactcaa ttctattctt attgcttatc tattccttcg ctgatttgca attcaattag
     2101 ttctaacgct ttatttttta gagaagcaaa gatataatcg tctttttgtt tgatacgatg
     2161 atctgttttt gctcttcgta tcaagtcacg taaagtcatt gatagctgtt ctctaacatg
     2221 atctacaaaa tacttgttat tacaaatttc atagataatt ttcagttgaa ttttttgttc
     2281 tttttcagca gatctaatcg catatttgaa ttttttgtac cagttgtcga attgacgata
     2341 atcactattg ctagccagtt ttaagcaatt cacaatactt tctccccata tttgtttaag
     2401 aagattttct tcaataaatg tttcgatcaa ttcttcatct aatttattat ttttagttga
     2461 aagtgattca agaagatcat cattatgtga ttctttaata tctttactat ctttataaga
     2521 cttatataat tctagttcat tttttgaact atcagcaaaa ttgtttttgt ctcttgtttt
     2581 cattgatgtg tcgcatagtt caaattttga attatcaaca gaatattgat ttgttccata
     2641 gttcaaattt tgaactatca agtttttttg tctattttct tgatgggtta tcttattaat
     2701 tttataaaca tcatctttgc acagaacagg ctttaacaaa taataaaaat ttgggaacat
     2761 ttgcatttta cttttattga agccattttt tcttttcaca agaagtttaa ctttttgaag
     2821 ttcatctagt gctgataaga ttgtattttt tgacattccc gaaatttcta cgagattttt
     2881 gatttcaaat atcaaaaaaa ttttttgatt ctcatcatac aagttatttt tcaatgaaag
     2941 tctatttcta tcttttagta acatataaac taacttagaa cttagtttca ttttcttata
     3001 tttttcattt acgtttaaaa aaataggata ttgatagtat aattctgcat atatatcaga
     3061 attagaatag ccttgctttt caactaagct ttcaacttct ttttcattca ttgtcacacc
     3121 tgcttctgat tttatttgct ataatagatt caaataaagt tttctcagaa ctttattatt
     3181 gagtgttccc ccgatctgca aactgacggg aacactcttt ttaatttttt ctacttcatc
     3241 aaatttaaaa aacgaggtgt tttttaaatt tgatgactca atatccattt tttgcattct
     3301 tcttttgaaa acaactccat tttttctaat aaaataactg gcaatcccat tcttttaaat
     3361 ttcaaaaaag ttgtatatga tacattcaaa tatttagcca tctcttttcc ttttagaaat
     3421 ggtctttcta atccggtatc aattcttgct tgatcaatag cctcttttgt catttcaaaa
     3481 acataatttt tcaattgatt cgtttgttgt ttatctaaca ttactgtaat tcctgcatcc
     3541 attatttttc tcctctcatt ttctatttct tcaatcagcc aactcgccaa gttgtattta
     3601 tttagatttt caaatcatta agtaaccaat ttaccaactt ttgataagta gatgctctaa
     3661 tactttcttt gctcccattt tcgattttga catatgtttt ccgatttata cctatcaatt
     3721 ttgctgcatc gtttttagta agtataagat cggctctatg tcttcttaag cttctaatga
     3781 attgatcatt aagagttatc attcagcgcc tcctttcaaa ttattcactt tgtggttttt
     3841 atcttaataa gcacatttta aaccacaaaa cgagtaatat caattaaaat attactcaat
     3901 ttgtggtttt catgctataa tagtttggaa tttactatag ggggcgttac tttgaataga
     3961 ataaaatttt atattgattc cagtaaaaag accattaaac aaattagtac agaaactggt
     4021 attaactata caactcttac gaactattac cgtggtgagc gaaatcctag aaatactgaa
     4081 acatggaata tccttgctga ttatttccat gttcctgtaa gttatttgat gggattagac
     4141 gaaacaaatt ccatagaaca acctaatctt gatagaataa aaactgctcg tttagaaaaa
     4201 aaggacaaaa tagtcaagaa tattgccagt cttgactcag attattttac tgaattatct
     4261 aatctatact tttccatgac ttctttactt gaagtcaaac ttcaagaaga tgactccgac
     4321 tttctttttt taaaaagtat tctagaggac tcctttgaat acacgattct atttgccaaa
     4381 caaaagaagt ttattaataa agctttaaac ttaaatatcg atgaaagaaa agagttagaa
     4441 tctgagttat ttggcaactt aacccaagta caggttaaca atctaaaaag agagataaca
     4501 gctcgattaa tatcaaaact tcaataaaac taattattca atcagccaac tcgccaagtt
     4561 atcagattgg agagattagt tatgtcaata caaaagtaca caacaaaaag cggtgcacga
     4621 tatagtgtcc gcttgtatgt tcgtaaagat gaaaatggaa agcaagaata ctatgtaaaa
     4681 aaagggttta aaactgaaaa agaagccaag ttacacgaag caagaaaaaa ggtcgagatt
     4741 gaagataccg gctatcataa gccttctaat gacttgttta taacagtgta tgaagaatgg
     4801 ttgccttctt ataggaatcg tgtacaagaa acgacatatc aacgtacaaa agaccttttc
     4861 cgcttgcata tactaccaaa attcgggaat aagtccattt ctaagattac tcctgttcac
     4921 tgtcagaatg cagtcaatga atgggcttta aaattcaaga atatcaaaca gcttaagtct
     4981 tacagctctc aaatatttga gtatgccatt tttgcagagc tattacaccg aaatcctatg
     5041 gcaaatacaa atctaccaaa gcgggaaaag aaagaaaatg aaaattactt ttcattagaa
     5101 gaactaaaag ctttttggga tattctcaat aaagaagagc ctttaaaaca tattttaatc
     5161 ttccagcttt tgattactac tggtatacgt aaaggagagc tagctgctct tcattggtct
     5221 gacatttatt ttgaagaaca gctaatgtat gttagaaaga gctatgcaac tattcgaaat
     5281 gaagagaaga atgcaagaag aaaaactgtc aggattcaaa aaaatacaaa aaatatatct
     5341 tccgaacgaa tattaccaat tgatgcacaa actattgaaa tgttaaaaat ctggaaaaaa
     5401 gaacaagctc gagagctttt gcagtttggc ataaatacaa atacaaaaaa tcaacttatc
     5461 tttacctatg taaatgcaga cggtgacatt aatcaacctt tgcatgcgga ctattcaaat
     5521 aatatattga aacgtttaga gaagaaatat aaatttaagc acgttactat tcatggattc
     5581 cggcacactc acgctactct tctactagaa ggtggagcaa gtatcaaaga aacacaagat
     5641 cgattgggtc ataaaaatgc tgaaactacc cttaacactt attctcatgt tactgaaaaa
     5701 gctcaacgaa atgcgttaga aaaattcatt gcttacactg gtttataata ttgtcattac
     5761 ctttctcatt accagaacga aaaaaagaca ccttctcgca atgagaaagt gtctgaaatc
     5821 gttggtataa caacgattaa cgttttgaaa actgtgaagc tttacgtgct ttcttaagac
     5881 ctggtttttt cttcgtatca ttgaataagt ttgaaaagcc ttgatttata aaggtttttc
     5941 cgtaaatata ttttgttata tttgattaaa tttgaaatgt ttgtgttcta aaatgtgttc
     6001 taatttaaag gcagtatatt tcgtatttta tattaaattt ttaggctcat tcattctcaa
     6061 atccattcta tttagtcaat aaatatttta aaaagacttt ataaacaaag atgttcaagc
     6121 gagcatctta tttttttgtg cctaatttga aaggagaaca tttcatgtat aaaaaacaaa
     6181 aaaagaaatt atgtgtatta actattttga gtttggctgt tcaatcaatt gtaggagcaa
     6241 ttgctcctac gattgctttt gctgatgaaa ttacccatcc acaaacggta accgtagaac
     6301 tagatttagc acatcaatat gctgttgaag gaacttttag tgatggtcgt ccaatgtcag
     6361 aagtcactgt tccgcattat gcagtgtaca acggcgttaa acaagatatt ttctgtgttg
     6421 aaccaggagt cccgatttac aatgagttca ctcctggata tgagaagaac ccgttaccag
     6481 atatgtccga taaagccaaa ttagtttccg ttttatggaa aaaagtaggt acagatgcag
     6541 atactcatat tgtggcacag aagatgatct ggcaagaagt taatgggtat acccttcact
     6601 caataaaacg atcagatggt agtgccgtaa atatcgcagc cattgaggca aaaatcaatc
     6661 aagccattgc cgattatcag aagaaaccaa gcttccataa tagtacggct aaaacagtat
     6721 tagggcaatc gactactgtg acggatacga ataatctgaa tttgtcagag ttcgatgagg
     6781 ttgtggaaaa taccgcaaac attgattatc gtgtgaatgg caatcagttg ataatcagcc
     6841 caaatgccaa ttcaaaagaa agtggtgtgt tgacgcttaa gaaatctgct ggtaccggaa
     6901 cacctgtcgc ctataaaaag gctggccaac agactttaat ggccggggcg atcgataagc
     6961 cgaacactta tacggtcaaa attgatgtgg aaatggaggg ttctttgaag attaaaaaag
     7021 ttgataaaga atcgggtgat attataccag gaacggtttt ccatttagac tttggaaaaa
     7081 ctttacccgc aaaagacgtg accaccgata aagaaggcat tgcgacctta gatgagattc
     7141 ctcatggtac aaaggtaacc attactgaaa aatcggtgcc ggcgccttat acgattgata
     7201 ccacacccat gactgccaca attaaagcag gtgaaaccat ttcagtgact tcaaaaaata
     7261 cgcgagaaaa aggtcaaatt attctggata aaaccggaat tgaaacaggg agtgatcttt
     7321 ggaacgataa ttactcttta gcgggcaata cgtttgccat tcgcaaggat agcccaactg
     7381 gtgaaatcgt ccaagaaatg accacggatg aaaaaggtca tgcagaaaca ccaaaagaga
     7441 ttgccaatgc gttggaattg ggaacctact atgtgactga aaccaaggct agtcatggct
     7501 ttgtgaatac tttcaaacca gtaaaagtcg aattgaatta tgtcaatcaa acagtggctc
     7561 ttgttaccag taacgtaaaa gggcaaaacc aagaaattac tggtgaaacc actttgacaa
     7621 aagaagacaa agataccggt catgaaactc aagggaaagc tgagtttaaa agagctgaat
     7681 acaccttgtt tactgttaag gatggaaaag cggtcaaatg gaccgagaac tttaaaccag
     7741 aattgttgaa gggaacgaaa gcttctgatg aaaccgtaac tttgacttta gatgaaaaga
     7801 atcaagttgc cgttaaacac ttagcgatta atgagtatta ctggcaagaa accaaagcgc
     7861 ctgaaggata taccttggat gaaacaaagt atcctgtatc catcaaaaag gtggatgata
     7921 gcgaaaaaaa tgctgtgatt actcgagatg ttgcggcaaa agaacaagtg attcgctttg
     7981 gctttgattt ctttaaattt gctggatcaa ctactggcac tgccgaaact ggcttcaatg
     8041 atctaacctt taaagtgtcg ccattggaag gaaccaatga aatcaccggt gccgaagatg
     8101 aagcgaccac agcttataac gagcaattag ggtttgatgg ttatggcaag tttgaaaatc
     8161 ttccttatgg ggattattta cttgaagaag tagaagcccc agaaggtttt caaaagatta
     8221 cgccattaga aatccgttct acttttaaag aaaataaaga ggattatacc aagagtgatt
     8281 atatctttac tattaccgaa aaagggcaaa agcaaccgat taaaacggta acggtccctt
     8341 atgggaatct aaccaatgag aatttctcag tcagtttgaa ccgtttgatg ctttatgatt
     8401 tgcctgagga agaagatagc ttaacttctc ttgcgacttg gaaagaagga gataaagaat
     8461 tgactactct tgattctacc gagctagtcg ataaattgag ctataatcta catgaaatca
     8521 aagaagactg gtatatcgta gctcaagcca tcgatgtaga tgctacaaaa gctgcccaag
     8581 aaaaagataa aaaagccaaa ccggtggcga ttgcggaaac aaccgcaact ttagcgaata
     8641 aagagaaaac aggaacttgg gagattctgc ataaattaac tgtcgaacaa gttttagaca
     8701 agaccattgt cttattcaac tatgtgtatg aaagcaaaga agcctatgaa gctggtgatg
     8761 agccggtagc gaaggatgct agcttgaaca atcaagcgca aactgttaat tgtatggtgg
     8821 aacgtcatgt ttccatccaa accaaagcgc acttagaaga aggttctcag acctttactc
     8881 atggtgacgt ggtagatatg tttgatgatg tatccattac ccatgatgta ctagatggct
     8941 caaaagaagc ttttgaaaca attctgtacg ctttatttcc agatggtaca agtaaagaaa
     9001 tttggaaatc tggcaagatt gattacgaag tgaatgacaa agaatttacc aaaaccgtac
     9061 ttgcggaaaa agtggataca agtaaatatc cagaaggtac aagttttacc ttcaaagaaa
     9121 ttaactatga caaagatggg aatatcaatg gaaaacacaa tgaagatttg aaagaaaaat
     9181 ctcaaacttt aacgcccaaa aaagttccga caacaccaag tacaccagaa caatctgaaa
     9241 tgccaactgt tccaagtggc tctcaagaat ctagtcctac agtgaaggca ttccctcaaa
     9301 ccggggagaa aacttccaat gtgctactgt tcattggctt tagcttgatc tttgcgacgg
     9361 ctggctatta tttttggaat cgtcggaact aaggtgataa gatgaaacat gtaaatagta
     9421 gaaaacaata cgcgcgaccg cctcctaaaa aagtggtcgc gctatttttg tattcaaaac
     9481 taacaatgaa aagaggaaaa aatatggaat tgaaatttgt tgtaccagat atggtcgaga
     9541 cgttcggaaa actaacatac ggtggtgaag gtgaagttct atcggaaggg tatggtcgaa
     9601 atactacaat tgttggtcga agttaccatc tgttttcaaa taaacaacgg gccgatgata
     9661 ttgaagtggt ggtagctgcg gaagccggcg aaaaggattt tgaccaagat caaccactaa
     9721 aagctgtaaa tccccactta attgccaaag gctatgaaat tgagaatcgt gggtttaccg
     9781 attacgtgtt gtatgttgat gatttagtga aagcatagga ggaaaagaag atgagattaa
     9841 cagaaggtat tgtcgtagat tcaggattaa cctttggcaa gttgcgtttt tccgcattac
     9901 gtcgagaagt acgcaagcaa aatgaggacg gaacggttag taacgaggta aaagaacgga
     9961 cctataattt gaaatcctcc gcgcaaggtc gaatgatcca agtcagtatt ccagcaaatg
    10021 tacccttacg cgagtttgct tatgatgcag aggtagagct ggtcaatcca atcgtggata
    10081 cggtcgccaa ttatgttttt cgggaaggaa ccaccgttaa ttggtttatc aaggcagatg
    10141 atttagtctt gaaacgacaa ctgaatcaag gaaaacctac gaaccaaaat gaaggaaaga
    10201 aataggtgaa gaatatggaa attaatgttg aacatatttt agattgtctc gatcaatttg
    10261 gcaaaggtga gctaacggag gaacaactca ctaacgcatt aacctatgat gaaaaaatgt
    10321 ttctgattat gcatcaaggg ttactggaag taggtaacga tgccaaagaa gactttgatg
    10381 tactggattg gttaggggaa gaagagtctt tctttatggt tgtcgaggta aatgaaagtt
    10441 tatgtcggca ggcagaatct gttttggaag aaatcgatgt ggagatgccg gatgccatcg
    10501 aaagattttt aaaacaatta gtggaaacga aacaacttcc cgtagtagta aatgactagc
    10561 ataacttttt gaaattgccc aatgaaatcc tattgggctt tttcgactgg aggtggaaac
    10621 catcaaaatg tacaaaggac atcgcatacg cgcaggagat caacacttga tttatcactt
    10681 tgttcttggc tggctaatct ttttgttcat tggttggatg agcgtctttt atttacaaga
    10741 acttagacaa tttgatattt ctaaggtatc attttccact agtgaaattg gctggtctat
    10801 gaaggatgta atctgcttgc taggaagtct cgctctttca ggaagcatga tgttacttta
    10861 tatacacttt tttcaggatc attggcgaag tctttggcat cgacaaaagt tagctcggat
    10921 gattctagaa aaccattggt atgaagtaaa gcagactcaa ggtgaaggtt ttttcaaaga
    10981 tttgaatagt agttggacca aagagagtat cagttacttc cccaaaattt attatcgaat
    11041 gaaagatggc ttactttcta ttcgcgttca aatttcactg ggaaaatatc aggatcagct
    11101 attgaagttg gaaaataagt tagaaagcgg actgtattgt gagttagtag aaaaagaact
    11161 caaagactcc tatgtggaat acaccttgtt atacgatatg attgccaatc gaattgggat
    11221 agaagaaata gtggcagaaa atggggcgtt gcgattgatg aaaaatcaag tatgggcgta
    11281 tgattccttg ccgcatatgt taattgctgg cggcacaggt ggtgggaaga cctatttctt
    11341 actcaccatt attcaagcac tattgaagtc agatgcagaa ttgtttatcc tcgatcccaa
    11401 aaatgcggac ctcgccgatt taggtacgat aatgcctcac gtatactccc aaaaagaaga
    11461 aatttctgaa tgtgtggaag atttttacga acgcatgata gctcgtagta ggtcaatgaa
    11521 agaaatgccc aactacaaaa caggagagaa ttatgcgtat ctcggacttc cgcccaactt
    11581 tttaatcttt gatgaatacg tggcttatat gggtgcgaat cgttttccta caagcatcga
    11641 atagatgtga aatggaagcc tattgagatt atcaatagtg acaactaatg ttttgagaga
    11701 tggaatgaaa gccttcaagt tgagctgaaa catccccgct aatactccta catgctctaa
    11761 tcaatgaagt agattggttt tggtatgaag ctaggtgaag tcgtctgaaa tctgcctaag
    11821 tgcttgagca taaggctgac tatagggcgg gcggctgaaa aagataacta tggtaagaat
    11881 gtctgaaaat gactgacgaa tctgcgaatg tacgggtcta aaaggctgat cttattgaaa
    11941 gataggaaca tacggacgta tggtgagata ccgacaagta aggttccatg atatgaaaat
    12001 cgggatagct aacgaagaag ctataatttc acaggctcat agtagatacc taaggttata
    12061 catgatagct aaaaacattg gaacgatgta agcagaaaac tatcaattaa atgactactt
    12121 ctagataggt gcatataagt aataacgaaa ttgctttgtt tctgtgaaag taagggcaca
    12181 gtaccgatga agcgtctaac aaatgtggag ggatagccct aagtctattt aataacaatg
    12241 aatgttgaaa cagatgaagt tcaaggatct ggtaagaacg tgggaaaatc cgttatggag
    12301 gtgccacgct tgaacacaaa aatacgttac tgggaatatt atgatctaca agaaacattc
    12361 gatcagctat ttactcaaag tagaaacggg aaaaagtttt atcaattata tgagttaatc
    12421 atttctgaaa ataatattct tttagcatac cgtactatca aggcaaacaa ggggtcttcg
    12481 acaccaggaa cggattcctt cacaattgat aactataagg aaatgaacca agcagagttt
    12541 atccatctta ttctcagtca tttagaaaat tacaaaccaa aatccataaa gcgagtaatg
    12601 atacctaaac ccaatggtga gaaacgccca ttaggaattc catgcatgat tgatcgaatg
    12661 atccaacaga tgttcaagca gatactggag ccaatatgtg aagctaagtt ttatgaacat
    12721 agctacggtt tcagaccatt gagaagtgca aagcatgctc taggtcgcat tatgtatctc
    12781 ataaacatca gtaaaatgca ctatgcagta gacattgata tcaaaggttt ctttgataat
    12841 gtaaaccacc gtttactgat aaaacaatta tggaatatag gaatttgtga caaacaagtc
    12901 ttagctattc tttcaaaatc tctgaaatca cccattcaag gagaaggtat cccgagcaaa
    12961 ggaacgatcc aaggaggtat catttctcca ctattatcta atgttgttct aaacgactta
    13021 gaccattggg tctcaaagca gtggcatacc tttgaaacaa agtatcccta tactaaaggt
    13081 tacaataaat ttcgtgctct aagagataca aatctaaagc aaggctatat tgtcaggtac
    13141 gcagatgact ttaaaatcat gactaatgat tatccgacag cattaaaatg gttccacgct
    13201 gtcaagcttt acctaaaaga cagacttaaa ttagatattt ccaatgagaa gtctaaaatc
    13261 gtcaatttgc gtaaatgtaa gtcggaattt ttaggattcg ctatttgcgt gaaacaaaaa
    13321 gggaaaaaat gggtttgtaa ttcacgtatt tctaacaaaa aaaaaggatc agattaaaga
    13381 agagattaaa caaagaatca aagatattca aaagagtcct acagcacaaa atgctctcct
    13441 gtttaatgct ctcgtgctcg gtgttcataa ttatttcagg tatgccactc atattaattt
    13501 agatttgaat aggatgaact acgttctttc attcactact tacaaccgat tatcaaattg
    13561 tagtacaaga tgttacccca acaaagcttc cccaacttac aaaaagtttt actcaacaac
    13621 gatgaaaacg tataaagtag ctggagtcta tttgtaccct ctttgcgacg tatctacaaa
    13681 aacaatttac ggatttaata cggaggatac accgtttact ccgtttggtc gtcaaagatt
    13741 ggagcacaaa agcctacagt ctcttgtgtt ccaagaatta agaaaattga tggaatcgaa
    13801 aataccgaat agaacggttg agtatttaga tagtagaata tctcgttact caatgaaatc
    13861 aggcaagtgt gagattacaa aacaattttt gccagcaaaa gccgttcact gtcatcacta
    13921 tttgccaaaa agtcttggtg gagacgacaa attcgataac ttgagaatta ttcataagga
    13981 tatccatctt ctgatccata caacaaataa aatgataatc gatcactacg tgcatgaact
    14041 gaaactggtg ccagagcaaa tagcaaaaat caacctttat cgaaagatgt gtaatctgca
    14101 aaacattcaa tagaaaataa atagatggaa cgccgtatga ggtgaaagtc tcacgtacgg
    14161 tgtggagcgg gggaaaagat ggagattata tcaaattctt acctatcgct atagatgtta
    14221 acgacaaaag aaagtgcggt gattttgaat aagcttaaac aaatcgtgat gttaggccgt
    14281 caatctggtt tctttctgat tctagcttgt caaaggccgg atgcgaaata tctaggagat
    14341 gggattcgcg atcagtttaa cttccgtgtg gctttgggtc gtatgagtga gcttgggtat
    14401 tccatgatgt ttggagaagt cgataaaaat ttttttatga aacgcatcaa aggacgaggt
    14461 tatgtagata cagggggcag tgtgattagt gagttttata cgccacttgt accaaaaggg
    14521 tatgatttct taagggaaat tagcaacatt gttggattaa ccgttcatac ggaaagagaa
    14581 aataatagtg tgtagcctag gaatatcaat aatcctgggc ttttttattg aggtgattta
    14641 tgacattttt agatttattc gccgggattg gtggctttcg cttaggtatg gaacaagccg
    14701 gtcaccagtg tatcggtttt tgtgaaattg atgaatttgc tcggaggagt tataaagcaa
    14761 tccatgatac aaggaaggag gtggaaatgc atgacatcac aagtgtatca gacgaatttg
    14821 ttcgatccct cggaccagtg gacatccttt gcggcggctt tccgtgccaa gctttttcaa
    14881 ttgcgggaaa gcggcaagga ttttccgata ctcgaggtac tttattcttt gaaatcgctc
    14941 ggttcgccac tcttctccaa cctaagtttc tattccttga gaacgtccgg ggattgctca
    15001 atcacgaagg aggggctacg ttcgagacga tcctccgaac gttggatgga ttggggtatg
    15061 atgtggaatg gcaagtgctt aactcaaagg cctacgttcc ccaaagccgt gagcggatct
    15121 tccttatcgg acattctcga gatgcatgta ccgaacaagt atttcctatc attggatcgt
    15181 cttcaacatc tgatcaaaac atcagaaact tggtaaatat caatccttcc aatcgaggaa
    15241 tgggtggcca agtctatggt tcagatagcg tagcaccaac cttgaccggt gatgaaggaa
    15301 ttaaaattgc cttaccggta aaggatggga tttctgtagc gggtatgttg tcgggaaatt
    15361 ttgaacaagg caataggatt tatgaggtaa ccggaacagc ccccactttg tcaacgaaac
    15421 aagggggaac gaagattatg attcggaaaa atgaaacaga atatcaagag gtgaagccag
    15481 gggacagtgt gaacctggct tttcctaatt ccacaagtcg ccgaggtcgt ctgggaaaac
    15541 aaagtgtgca taccttacta acaggagacc aacaagcggt agtcacggat cagtaccaga
    15601 ttcgaaagtt aacacctagg gagtgctggc ggcttcaggg ctttccagat tgggcttttg
    15661 atcgagcttc tcaagtcaat tcagatagtc agttgtataa acaggccgga aactcagtaa
    15721 cagttccggt catttttgat attgctagaa gattaaaggg ggtaaaagca tatgatttat
    15781 gacgagttga ttggagaaat ctactggatg attggcaaga ttcaggcaga tccgaaattg
    15841 gaagaagaac tgtaccggct caattttgat attcgcaaaa acggggtgaa agtccaggtg
    15901 attcgtatgt agtggatgag gaaacggatg ctcgaatgga gctcaatcaa gtcatcggtg
    15961 aattggaacg aattgcggac cttgccaaag aacctgatat ccgccaatac ttgtttgaga
    16021 taaaagcaga gttggaaatt gagggtatca ccgcggagta atagggaaat aacccactga
    16081 taggcactga ccgcggggga tttgcgaagc agaaatcctc cgtggtgctg tgcctgttca
    16141 tggacggcgg cagccggcca tgatgtctca ccccccgtat ctaacagggg ggtacaaaaa
    16201 cacaaaaaac aagcaagtaa ccataaaaaa tgcttgttgt atcaaggata ttaagtaatt
    16261 tgtataggtg ttaactcatt tggaaaagtt aacaccttga atttggaggt gaaaacttgg
    16321 cgcaacgaaa tttagattat cggttattaa aagaacgacg caacgaatat ggcgtttcac
    16381 aaaataaatt agctactgct tgcggactta gccgcccata cctaaatcag atagaaaacg
    16441 gtggcgtaat cgcctctaca aagaccatga gaaaaatttt tgatcaactg gaaagtttca
    16501 atcccgattt acctttaacg ttactatttg actatgtaag gattcgcttt cccacaacgg
    16561 atgtgcggaa aattattcaa gaaattctcc atttgaaatt cgagtatatg cttcatgaag
    16621 attacgcctt ttactcctat caagaacaat atgtcatggg cgatattgta gtgatgttat
    16681 ctcatgaaga agataaaggt gtccttttgg aattaaaagg tcgtggttgc cggcagtttg
    16741 aaactttttt actcgcccaa aagcgtagct ggtacgactt ttttgaagat tgtctaaaag
    16801 ccggtggcgt gatgaaacgc ttggatttag caatcaatga tcgagtgggg ctactggata
    16861 ttcctgattt aacgaaaaaa tgtcagaagg aagaatgtat ctccttgttt cgtaccttca
    16921 aaagttaccg ttccggggaa ctgttgaaag ctgatgaaaa ggatggtatg ggcaatacct
    16981 tatatattgg cagcctcaaa agcgaggttt atttttgttt gtacgaaaaa gattatgaac
    17041 aatatatcaa gttagggatt ccattggacc agaccgaaac gaaaaatcgt tttgagattc
    17101 ggttgaaaaa tgaccgtgct tattatgcga ttcaagattt gttaaaaggt cgtagcattg
    17161 aaagtaccac gttttccatt atcaatcggt atttgcgatt tgcggataaa gtagatggaa
    17221 aacggcgaac caattggccc ttgaatgaac aatggggtcg ttttattgga cggaatcgga
    17281 aagagattca attaacttct gaaccgaaac cgtataccat tgaacgaaca ttaaattggc
    17341 taggtagaca agtcgcccct acgtggaaaa tggctaagga gttggatcga ttgaatcaaa
    17401 caacgtatat ccaggatatg gtacagaatg cgcgactctc ggaccggcat aaaaagattt
    17461 tggagcaaca aagtatggca atcgaaaact tgattgtatg aaaagaggaa ttttgatgga
    17521 aattgtaaaa gatatgttat tggtacttgg tggcagtatg cttggtgtta ccgtgatgtg
    17581 cttgatgaat gcaagtgcag cagcggattg agcaatggaa aagaaaggaa agtgacaaaa
    17641 tgaatttcgg acaaaa
//
LOCUS       DACGLW010000062        17327 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.065, whole genome shotgun sequence.
ACCESSION   DACGLW010000062 DACGLW010000000
VERSION     DACGLW010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17327
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.065"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            155..1414
                     /locus_tag="IXT53_002030"
     CDS             155..1414
                     /locus_tag="IXT53_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAQ4905829.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            1420..2001
                     /locus_tag="IXT53_002031"
     CDS             1420..2001
                     /locus_tag="IXT53_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAQ4905830.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(2083..2958)
                     /locus_tag="IXT53_002032"
     CDS             complement(2083..2958)
                     /locus_tag="IXT53_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ4905831.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            3106..3438
                     /locus_tag="IXT53_002033"
     CDS             3106..3438
                     /locus_tag="IXT53_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAQ4905832.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            3550..4758
                     /locus_tag="IXT53_002034"
     CDS             3550..4758
                     /locus_tag="IXT53_002034"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAQ4905833.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            4912..5367
                     /locus_tag="IXT53_002035"
     CDS             4912..5367
                     /locus_tag="IXT53_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905834.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            5729..6004
                     /locus_tag="IXT53_002036"
     CDS             5729..6004
                     /locus_tag="IXT53_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905835.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            6017..8095
                     /locus_tag="IXT53_002037"
     CDS             6017..8095
                     /locus_tag="IXT53_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAQ4905836.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            8255..10141
                     /locus_tag="IXT53_002038"
     CDS             8255..10141
                     /locus_tag="IXT53_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ4905837.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            10153..11100
                     /locus_tag="IXT53_002039"
     CDS             10153..11100
                     /locus_tag="IXT53_002039"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAQ4905838.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            11120..11647
                     /locus_tag="IXT53_002040"
     CDS             11120..11647
                     /locus_tag="IXT53_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ4905839.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(11710..12363)
                     /locus_tag="IXT53_002041"
     CDS             complement(11710..12363)
                     /locus_tag="IXT53_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAQ4905840.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            12495..13337
                     /locus_tag="IXT53_002042"
     CDS             12495..13337
                     /locus_tag="IXT53_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ4905841.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            13495..14391
                     /locus_tag="IXT53_002043"
     CDS             13495..14391
                     /locus_tag="IXT53_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ4905842.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            14394..15107
                     /locus_tag="IXT53_002044"
     CDS             14394..15107
                     /locus_tag="IXT53_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAQ4905843.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            15127..15645
                     /gene="msrA"
                     /locus_tag="IXT53_002045"
     CDS             15127..15645
                     /gene="msrA"
                     /locus_tag="IXT53_002045"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ4905844.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            15642..15869
                     /locus_tag="IXT53_002046"
     CDS             15642..15869
                     /locus_tag="IXT53_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAQ4905845.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            16021..16572
                     /gene="lepB"
                     /locus_tag="IXT53_002047"
     CDS             16021..16572
                     /gene="lepB"
                     /locus_tag="IXT53_002047"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ4905846.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(16655..17197)
                     /locus_tag="IXT53_002048"
     CDS             complement(16655..17197)
                     /locus_tag="IXT53_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAQ4905847.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
BASE COUNT         6054 a         2935 c         3559 g         4779 t
ORIGIN      
        1 gaaaaagttc tttttttggc agaaaaaagc aaaaaaatac ttgttttaga aaaaataggt
       61 ataatagacg gataccaaca tccaaacgaa aaagaattat tgagaacaat gacggtattt
      121 caacaaatcg tcagtgagat aagagggaat tgaaatgaat acaaatagcg aaaaaatgtt
      181 gcaagcttta tcagatgaag acttgtcaca agcacaaatc tttttagaaa aagcattaaa
      241 agaagaccca gcagatatat tagctgaact aggcgaagaa ttgctggcta ttggcttttt
      301 gatcgaagca aaacagattt ttgaacaact gatcaaccaa tacccagaaa atgacggact
      361 gaacatcccg ttagcagaaa ttgcaatcga agataatgag atcgacttag cttttgaata
      421 cctggaaaaa gtggataagc aaagtgatta ctatccgcaa agcttattgg cgatagctga
      481 tttgtaccaa gttatcggaa tccctgaagt gagtgaagca aaattgaaag aagcagctgg
      541 gttgcttcct gatgaaccat tgatccagtt tgcgctagct gaattgtatt tttcagttga
      601 tcgtttcaaa gaggcagcag tggtttatga aacattgttg gcaagcggga taaatgaaat
      661 ttcaggaatc tcgatgcagg aacgtttagg acaatcctta agtatgcaag gagaattcga
      721 agcagctatc gtacctttag aagaagcact gaaagaagaa aggacagatg atcgactgtt
      781 ccagctagct tttacgaatc ttcaattgaa agaaaatcag caggcaatca actatctgga
      841 agaacttcga gaagttaatc cgcattatca atcgttatat ctatatctag gacaagctct
      901 tcaagaagaa gaaatgattg aagaagcaca aacagtgtta gaagaaggaa tcaaagaaaa
      961 tccttaccaa gtcgaacttt atcacctggc ctcggaaaat gctttccgtc tgcatgatag
     1021 acagcgagca gaagaacttt tactgaaagc tttagaagta ggagacaaac aagatgaaac
     1081 attacttact ttaagtaatc tttacttgga tgatgaaaga tatgaagatg tgatacaagt
     1141 gatcaatcag atggaagaaa ctgctaatcc ttatgcagaa tggaacctag cccatgctta
     1201 taatgaatta gaagacttta ccgttgcagc tgtacactat gaacaggctt atcatgaact
     1261 atcacatgaa ccagatttct tgaaagagta tgcctttttc ttaagagaag aagggcagct
     1321 gaaaagagcg caagaattat tgacttatta tttaacactt gaaccaggag atatggaggc
     1381 actgtcatta ttagatgacc taacagagag gtgagaaaaa tgctaattga tgtacgggaa
     1441 aagaagaatt ttttaacgtg gatggtcaat catgtatctt ttagccgaag agaagttttt
     1501 tggatcttga attatcttgc caatcatgaa gcgattttaa gcaatgtcca ctttgttgaa
     1561 ggggcgaatt ttgcggatcg aggactgcag atcagtgatc tatctgttga aggggaaccc
     1621 atgaaattgt ttttgcaggg aaaagaattt acggacacag accagatttt tcatgaaatc
     1681 cgtttgaatt ggaaaaagcc tttatatgtg gaatgtatat tcgaaaatgc ttggcagaca
     1741 agagaatatc tatctatctt agaagacaat ccattttctt cttgggattc ttcagtcagt
     1801 gaagaagata ctgaattaat tgaacgatat tttcaagaaa aagaagaaga ggcaaaacta
     1861 aaaatgcttt atgctcaaat cgatcaagca ctagaagatg gagataggga tgcttttttg
     1921 aaattatctg atgaagttaa ccgaaagatc cttttgcaga cacaaaagaa aagtaaccgg
     1981 gtaagtgaac ataacaatta gttaaaaata gtatagtact aaaaaagaca aaggtcagct
     2041 ggatgtggaa tctttcctca tagcctatct ttgtcttttt tcttatagtt ttttcagtaa
     2101 tcttgtctgt ggcttcaaat aagtaaagcc ttgcccttcc acttcatgca cattgatgat
     2161 tgaaaggaaa gctttttcgt caatctcgtg gacgatcctc ttagcttcag ttaattcact
     2221 agagctaacg accatataga tgattttttt tgaattacca gagaatgcgc cttctccaga
     2281 aaggaaagtc acacctcttt gcagaccggt catcagtagt ggtgcaatct cttcgctttt
     2341 gttggagacg acaagtagac cttttgcaga atatccgcca tctaagatcg aatcaatcac
     2401 acgactgaaa acatacgaaa caatcaacgt atacatcata cgtttcaagt cgatataggt
     2461 taatgaagca agtaagacga tgatatcaaa tgccaatagg gaacgcccca tagtgatgcc
     2521 gtaattttgt tctaaaatcc gagcgataat atcactgccg cctgttgttc cacccatgcg
     2581 gtagacgatc ccgcttccta cacctgcaac taaacccgca agtagagaaa cgatcagtag
     2641 atcatgctgt aaattgattt ctagtggaac cctttgccag atccataaaa agaatgacaa
     2701 agaaagtgta cctagaacag tataggccaa ggaacgttta ccgaggatct ttcctcctag
     2761 taaaatcaat gggatgttga ttaataaagt cgtataagct ggatcaatac caaataaagc
     2821 tcttaagatc aaggtgatcc ctgagactcc cccttctgct aaatgattag cgatgttcaa
     2881 gtaaacaaga ccaaatgcat aaatacttgt tcctataatg ataaaaagaa cttcttttac
     2941 tgtttttcgt ttgaccatat gaattccagc tttcaattat gtattttcac agaatttcca
     3001 tttagaaatt ccttcctaaa tgtagcatgg aaattcgatt ttctcaattg ctcttatcta
     3061 ggatttgcgt taaagttttg gtaagatagt tttggaggga aaaagatgga taaaaaacgt
     3121 tctttataca gtatgcagca ggaagtggat gaatatatcc agcaattcaa ggtggggtat
     3181 ttctctccat tagctcagat ggctcgatta actgaagaag taggtgaatt ggcaagagaa
     3241 gtcaaccaca cttatggaga aaaaagcaaa aaggcttctg aaccagttaa ctcggtagca
     3301 gaagaacttg gtgatgtttt attcgttacg atgatcatgg ctaattcatt gaatatcgac
     3361 ttaactgaag tttttgaaaa aaacatggaa aaattcaatc aaagagacca taatcgcttt
     3421 gagcgtaagg acgagtaaac aagatgaagt ggtcctggcc ttgaagactc tgaaacggac
     3481 ttgtcaaaca gtcaccatgc aactgagtct ttatctcagg acaagaaaca cgcaggaaag
     3541 gttggaaaaa tgaaattaaa acaattacct ctggaatacc aaaaggcaat tccagttctg
     3601 aaaaaattag agaatgcagg atatgaagct tattttgtag gtggaagtgt tcgtgatatc
     3661 ttgctgaacc agccaatcca tgatgtggat atcgcaacaa gcgcatttcc agaagaaatc
     3721 aaacagcttt ttccaaaaac gatagatatt gggatcgaac atggaactgt gttggtatta
     3781 gaaaatgatg aacaatatga gatcaccaca tttcggacgg aatcaactta tcaggactac
     3841 cgtagaccag atcatgtcga attcgttcgt tcgttggcag aagatttgaa aagaagagat
     3901 tttacgatca atgcgtttgc tttgaaagaa gatggagaaa tcatcgattt gttcgatggt
     3961 ctgcaagatc tagaagaaca gatcttgcga gctgtaggca atcctcatga gcggttccat
     4021 gaagatgcct tacgaatgat gcgcgggttg cgttttgtca gtcaactagg gttcaaatta
     4081 gaggaagaaa ccttctacgc catcaaagaa aaccacgagc tattaggtaa aatatctgta
     4141 gaacgtataa atgtggaatt catcaagatg ctcttgggaa atgaacgggc cgcgggaatc
     4201 catgcattta ttgaaacaga gtgctacatt tgctgtcccg gtttgaaaga agaaggcgaa
     4261 gcacttttac gttttgctga attaccatcc gtcccattga aagaagagag ccaagcatgg
     4321 gcattattga tcagccaact ggatctaaaa gaaaatgaga ttcgttcctt tttgaaatcg
     4381 tggaagtgtt ctaaccaaat gattaaagac gttcaagcac ttgtccaagg actacgacaa
     4441 cgagaaactg gagctttgga accattcgaa ttgtacttct taggcaaaaa acatgcactg
     4501 caagtagaag aattgatgat ctattttgac gaaacacctc agatggatgc cgtaggagaa
     4561 tcctatgatc gtttacctat caagtcatta agcgaattag cagtagatgg taaaatcttg
     4621 ctagaagaaa ctggaaaaaa acctggtaag tgggttagtg aagctttgca attagctgaa
     4681 aaagccgtcg tagaacaaag aataaaaaat gataaaaaac aggttttgga ttttttatcg
     4741 aaagaaaaat tgatttaaaa aacactagac tgtatctgct cagctttgtt ggctacttaa
     4801 agtgggcaga tacgtcatta gaagaaaaaa cataagatgt gaccacttac ataaatttgt
     4861 gctaaactga ggtagtacaa gaataaatta catcataaag gagtcttttt aatgagtaaa
     4921 gaaatgacag cgctaaaatt ttattttaga aatggagaaa cctggacgat caaccgccgt
     4981 catatcggcg atctttggat caaacaaatc acaacaagtt ttggacgaat caatggcagt
     5041 gaatttgttg aaatccatcc gtgtgctgga tttaaaatcg aaatcttcca tgaaggggac
     5101 gcagttgcga cacatgatat caacctaggc ggactggaaa tgggcatgtt taatcgcgca
     5161 ttgaaatacg aagatatcga gcgaatggaa atcctttacc gaaatggcac acctgatctt
     5221 gtgtacttcc catatctaga taaaggaaca gaaggattag acaatcaata tcaatcaaca
     5281 aaaatcagcg aaaaaacagg taacttgtat atcgtgatca atccagatca acgtgtagaa
     5341 gatgtatacg gggaattttt cgaataaatc gatttttacg caaaaatgag gctgtgaaag
     5401 agatgtgtct tattactact gataggctgt ctcttccaca ccgtttattc agaataaggg
     5461 actgtgacaa gatttttgtc gcagccccat ttttttgttg tgataaaaga acaagaaagg
     5521 caggaatggg aaagtaaaca tccttttgtc agaaactttt atctttctta agttttactt
     5581 gatatctgat actatttgga atagaatgag gcgttgaaaa atttatggaa atttctaact
     5641 agcaataaac gatgaaacaa tgaaatattt ttcatccaaa aagaactttg ctataatgat
     5701 ggaagtttgt gtataggaga tctggaaaat gagagagagt tttgaacaac aaaaaaaact
     5761 gttgcatgat cgctatggcg ccctttcgat ggatgaccgc cgtcaaatac tttgtaagtt
     5821 gcgtaaaaga aatatattga tgtatcgtca gttggaacgt ctgaaacatg atctgcttcg
     5881 tttggagtca aaacgtgtac agtgtgaatt agaggggaac caaacacagg ttgaagtagt
     5941 cgaaaccaaa attttaaaga aaaaagaaca atttttaaaa atgcttacac aaaataaaaa
     6001 ataaagggga tggacgatgg aatttgctga gttagttatt gtatttgcag taacgatcac
     6061 cttatcgaac atagctagcc gcattttgcc aatgattcca gctccactga tccaaatttt
     6121 ccttggagtg gtattaggtc tgacagagtg gggacaatca atcgattttg aacctgaatt
     6181 gttcttagtt atgattattg caccattgtt atttagagaa ggagaaaaag cagatatttc
     6241 ttcgattctc aaaaatttcg ggacaatttt gttcttggca tttggaggag tcattttgac
     6301 ccttgtcggg gtaggtgcga cgttatcatt cctgttgcca agtgtcccac ttgctgcctg
     6361 ctttgctttt ggagcagcgt taggtccgac agatgcggta gcagtcagtt ctctttctgg
     6421 acgagtgaat atccctaaaa aggcgatgca tattttggaa ggagaaggtc ttttgaatga
     6481 tgcatctggt gttactgcct tccagtttgc tcttggtgcg ctgatcaccg gatctttttc
     6541 agcagtaaac gcggggatga gtctagtagt ttctagtatt gggggagctt tgatcggctt
     6601 tttgcttgtc tggttcaaac aaaaaataat tcatttgatc gaaaaagcat cagcacaaga
     6661 tgtcacaggg tatctgctga tcgaactttt gcttcctttt ctagcctatg tattggcaga
     6721 ggcatttgat gtatctggaa tcattgctgc agtggcagca ggtattctcc aagcttctgg
     6781 ttttaggaaa atctctgtgt ttgatgcaga attgtcgagc ttgtcccaca gtacatggac
     6841 aactattgct tttacactga atgcattagt atttatattt ttaggaatcg aattgacgca
     6901 agtgttctcg ccagtatggg gagacggttt atatccaaac ggtcttttat tagcgattat
     6961 cgtattaatc tctgtcatgc tttttgtgat cagattcatt tctatcagtc ttttttatgt
     7021 attcaaagat ggcagtaaaa agttcaaaaa acagttgaac gagatcttga tcttaacatt
     7081 tggcggtgta aagggaacag tcagtttggc tacgattttt attttgccat tttcaatcaa
     7141 taatatgatg ttttaccagc ggtctttgct tttgtttctg acggcgggtg tcatcttagt
     7201 gactttggtc atagggatca ttgtcttgcc tatgctgacg gaaacagagg aagcgaaaag
     7261 cacggatttg aatgctttga tgattttaga agaggtcgtt gaagttttgc gaaaagaaat
     7321 aaaggaaatt gatcgtaata caaaagaatt tttagccact gaagcagtga tagaaaatta
     7381 tcaagaacgg atacgtgatc tgtacttgga agatttaacc gatgatgaaa agcaagaagt
     7441 acaagaaata caagcactga ttttgtcgat cgaacgggat ggattggatg aaagttatcg
     7501 ttcgggcaag ctttcttcta atggttatcg tttttactcc cgttttcttt ctcgattcga
     7561 acattctatc acaagccaga ttttgtcttt tatcggcttt tggttcattg ttgtccgtag
     7621 gttaatgcga atcgttctcc atccgaaaat gttttggcaa agaagatatg ctgatcgtca
     7681 gacatttatt agtgatgaag atatccaaga aatcagagag acatatcaaa aaaatactca
     7741 gttgattatt gaaagtttag ataacttgac tgatatttat gatgaaacga tggttcaatt
     7801 ttttatccag caacgcaaac ttgaaggaat aaaaatgaac tcggggaatt tgatttcttc
     7861 gtggatgatc cagcaagata gtctgtttac gaaaaaaatg ctaagaggtt attatcttga
     7921 aagaaaaatc attgatgaat acgaagttgc tgaaaaaatc acgacgtttt ctgcgaacag
     7981 ctatcgcagg aatatcaatt tgttagaatc ttatacgatg aacaaaccat ctgataactt
     8041 ttctttccga tttgcgtttc gagcaaagtc aaaaaaaacg agtccaataa aataaaaagg
     8101 taaaaatttt caagttttat aaaaaacaaa acatatcaga acaggatcga atcggtcctg
     8161 ttcttttttt gagaacacaa aaagaacaaa taccaaaaaa attttcctga agttcactat
     8221 ttttatgata caatatccaa gataaggagg gaacatgttg aaagaattaa aagtaactga
     8281 cctcaaaaaa acttatggtg aaaaagattt gtttgaccag atttcttttt tggtccacga
     8341 aaaagaccgt atcgggttga ttgggacgaa cgggacgggt aaaacaagtc tcttaaatat
     8401 tattgccggt atcgatagca gcgacggtga tcggcaaaca gtgttttatc caactgatta
     8461 ccgtattggt tatctttctc aagcagctga attttccgat gaattaactg ttctccaagc
     8521 agtctttcaa ggaaacagcc ccttgatcca aactgttcga gcatatgagg aagcattgat
     8581 cgaactaggg gaaaacggag aagaccctga tgtgcaaaaa cgttatgcta aagcagaaga
     8641 acaaatgaat aaagaagatg cttggactac agataccaac gcaaagatta ttttgcaaaa
     8701 attaggaatc agtgaattag ataaaaaaat cagtacttta tcaggtggtc agaaaaaacg
     8761 agttagtctg gcgcaagtgt tgattgatga acccgattta ctattactag acgaaccaac
     8821 aaaccattta gattatgaag cgatcgagtg gttggaaaac tatttgaaac aataccgtgg
     8881 cgcactattg atggttaccc acgatcgtta ttttctagat agagtagcaa accgaatctt
     8941 cgaactttca tttggaaaat tatacgagta taaaggaaat tatgaagctt acgtcttaga
     9001 aaaagcagaa cgtgatcgtg tagaagtaga acaagaagaa aagcgcaagc ggctttataa
     9061 acaggaatta gcttggatga gggctggcgt acaggcacgt ggcaccaagc agcaagcacg
     9121 gatcaaccgt ttcgaagatc taaaggagaa cttgtaccaa gtaaaccaag aagatgattt
     9181 agagctgaac ctagcgacac aacgtttagg gaaaaaagta ctggaaatca aagatggcag
     9241 ttaccgtata aatgaccaaa cactgctaga acatttagat ttattgattc aatctcggga
     9301 aagactaggt attactggta aaaatggtgc gggaaaatcg acattgctta atattctagc
     9361 tggcagaatt cccctggata gcggtacaat gtctatcggt gaaacggttc atcttgcgta
     9421 ttatacacaa gaaaatgaag aaatggcacc agataaacgg atgattgctt acttacaaga
     9481 agctgctgaa gaagcaaaaa cagcagatgg cagtcaaatc ggagtagcag aattacttga
     9541 acgattttta tttccaagat ttatgcatgg gacactgata ggcaaacttt caggcggaga
     9601 aaaacgccgg ttatttttgt tgaaactgct gattcagcaa cctaatgttt tattattgga
     9661 tgaaccgacc aatgatttgg acatagcaac attgacgatt ttggaagatt attttagaag
     9721 ttttccaggt gctgtgatca ctgtttccca tgaccgttat ttcttggaca aggtagcaga
     9781 taagttatta gtattccaag gaaacggaaa acaagaactt tattacggaa acatgtccag
     9841 ttatttactg aagcaaaaag agacccagca gcctgctgaa aaagcgaaac caaaaataca
     9901 gagcaaggaa cctgctgaaa agaagaaatt atcttacatg gaacaaaaag aatgggaaac
     9961 gattgaagat gagattgcag aacttgaaga aaaaatctca ttgctccaag aagagatgaa
    10021 tcatcaagga gataatttca ctcgattaca agaattgcaa aacgacgtat ctgaaacaga
    10081 agcacaactt gaagaaaaaa tggcacggtg ggaatacttg agtgaatggg tagaagatta
    10141 ggaggaaaaa ggatggaaca agcgtatctt gacttaggaa gaaaattgtt ggaagaaggg
    10201 catcgtaaag aagacagaac aggcacaggc acattaagct tattcggata ccaaatgcgg
    10261 tttgatctgc aaaaagggtt tcctttgtta acaacgaaac gagtaccttt tggattgatc
    10321 aaaagcgaat tgctttggtt tttaaaagga gatacgaata tccgctatct attacaaaat
    10381 aaaaaccata tttgggatga atgggcattt gaacgctaca tcaaaagtcc agactataat
    10441 ggaccagata tgactgattt tggccataga tgtttgacag atcctgaatt caatgaagaa
    10501 tataaaaagc agagcaagct tttttgcgag aagatcctaa ccgatgattc ctttgctgaa
    10561 acatacggcg atcttggaca catctacggc tatcaatggc gtcactggga aacaaaagac
    10621 ggtggcttta tcgatcaaat caaacaagtg atcgaagcaa tcaaaaagac tcctgattct
    10681 agaagattga tcgtttctgc atggaatccg gaagatgttc ctagcatggc tttgcctcct
    10741 tgtcatacga tgtttcaatt ttatgttcaa gagggacggc taagctgcca attgtatcaa
    10801 cgcagtgggg atgtgtttct aggcgttccg ttcaacattg caagttatgc gttgctgact
    10861 cacctaattg cacatgaaac aggtttagcg gttggcgaat ttgttcacac attaggagat
    10921 gctcatcttt atatcaatca tatcgaacaa atgaaagaac aattgagccg agaaatacgc
    10981 gcatttccga ctttagtttt gaatcaggaa aagcagtcag tatttgattt tgagatggaa
    11041 gatatccaat tagaaggata tgacccacat ccggcgatca aagctcctat cgctgtttag
    11101 aatcagaaag gatgagaaaa tgttagctgc aatttgggca caggatgaaa acggattgat
    11161 tggaaaagaa gaccgtcttc cttggcgttt gccaaatgat ctgaaattct tcaaacaaat
    11221 gacagaagca aatacactag tgatgggacg aaaaacattc gaagggatgg gtagccgtcc
    11281 gttgccgaat cgtcagacga ttgttttgac gagagattct tcctataaag cagaaggcgt
    11341 gcatgtcatg catagtgtgg aagacgtgct tgctctggaa aaagaaacag acggtatctt
    11401 tttcatcgct ggagggtcag ctgtttatca agaatttctg ccattttgta cgatattata
    11461 ccgtacagtc atccatcatg tattcgatgg agatgcttat tttccacctg ttgactggtc
    11521 tgattggtca ctgatcaata ctagtcaagg agaaatggat gaaaaaaatc gttatccgca
    11581 tcaatttgaa acgtatcagc gtaatgagaa tcctgtaaaa agcgggacag gcgaattgac
    11641 gatataaaaa agctatggat agactagctg ttatttagag ctgtcccatc catagcttta
    11701 ttttttttat taagtaaaga ataaaacaga gaagtacata aaacctgcac ctaacataac
    11761 aaacaaatgc caaacaacat gcatatagcg gatagaattc aagctataaa agaatgcgcc
    11821 tatcgtgtaa gaaagcccgc ctgctaccat caaaataaca ccagtcattc caagagagtg
    11881 ataaagagga acagcagcca cgacacagag ccaccccata aaaatataga tgatcgtaga
    11941 tacttttgat actctctccc gtttatgcaa cgtcaacgct ttatatataa tgccaagaat
    12001 cgcaaacaac cagattaata caaataaagt ccaacctagc catcctcgga tactcagcaa
    12061 acaaaaagga gtataacttc ctgcaatcaa aagaaaaatc gaactatgat cgaatacttg
    12121 gaacaccttt tttgcttttg taaaaatcaa tgaatgaaac aaagtagacg ttaaaaataa
    12181 aagaatcatc atcgatccat agatagcata agagacaata tggagggagg aatgcaagtg
    12241 ggcacctttt actaaaagaa ccactagtcc cgcgatactc aatccagtcc caattccatg
    12301 ggtgactgca ttaaacactt catttaatat ttcatacgtt cgattaaatt tcgttttttc
    12361 catgaaaacg gctcctttac taggtgtaat taatgaaaaa tcattatttg tctgttatac
    12421 taaaaataga aattgtctta catgtagttt aacatgaata aggcatcttg ttaaagaaag
    12481 agagagagaa aataatgaca aatgtaaaaa tcgtaacaga ttcttcatgt acgatggaaa
    12541 aaaatgtacg cgatgcttta ggcattcgtg tcatgccgct atccatcatg atcgacggaa
    12601 cagtatatcc cgatgatgac caactagacg gcgaaagatt tatggaaatg atggcgaaag
    12661 caaaaaatct gcccaaaaca agccaaccac caattggaga attcatctcg atgtatgatg
    12721 agctaggaga agacggcagt gaagtgattt cgatccatat gacaaaaggc ttgagtggaa
    12781 cagtagaagc agcaagacaa gccagtaatc taacaaaaac gaaagtaacg gttatcgaca
    12841 gtgataccac tgaccaagga ttatcttttc aggtaattcg agcagctgaa ttagcaaaag
    12901 aaggtaaaac agtagagaaa atccttgaag aagtcaaaaa agtacaagac aatacaaaat
    12961 tatacatcgg aatatccaca ctagataact tggtaaaagg tggaagaatc agccgaacaa
    13021 ctggtttgct gtctagtgtt ttgaacatac gcgtggtgat gaatttcgcc cattctgaat
    13081 taatccctgt aactaagggg cgaggaaaga aaacgttcga taaatggttt aataatttga
    13141 aaaatgaact gaaagaattg ccaaatgtgc gacaaatcgg tatttcacat gcggatgcaa
    13201 aagaattggc gcaagaattc aaagagggtt tgcaagaatt attcccggac atgcatatcc
    13261 ctgtactgca tacgaatccg gttatcgcta cgcatactgg taaagacgcg tttgcaatca
    13321 tgtattatgt agattagtcg aaaagaggat aagccctcag cctgaaaggc ttgcagctta
    13381 ttctcttttt ctttataaaa ttaggaaaat aattgttttc ttttttcata aagactacct
    13441 ttttgataaa atttttgata acattagaac caatcgaata caagaggtga aaaaatgaaa
    13501 ttaacgaaac gacatttgat cctcgttttg ttcccattat taattgcgat catcatctat
    13561 gcaggtttag ctgcttctat accgaaagct ggcccactaa tgaaaccagt aactactgtg
    13621 agagaagatc caaataaaaa agaaaaactt cactacatag ctatcggaga ttcgctgact
    13681 gaaggagtag gagatcaaac caagcaagga ggattcgttc cccttgtcgc aaatgatcta
    13741 aaagaccgtt ataatttgac cgcggttgaa acagaaaact atggtgtaaa tggtgaacga
    13801 agtgaccaga tcttaaagcg agtaaaaaca aaagaagaaa ttcgaaacaa tatcaaaaca
    13861 gctgatttta tcacactgac tgttggcggt aatgatttga tgaaagttat ccaaaataat
    13921 ttattcgggt taacggtcaa ttcctttaaa aagccactga aaaactatca ggaaaatgtc
    13981 acagaactat tggaagaatt tcgatccctc aataaaaccg cacctattta cattttaggc
    14041 atctacaatc cattttattt gaactttcct gagatcactg acatgcagac aattgttaac
    14101 aattggaaca aaggaacaga aacggtgaca aaaaaaatgc cgaactgcta ctttatccca
    14161 atcaatgatt tgctttatca aggattgaat caggaagtag gtgtcacaga agaaggaaca
    14221 gatcaaacaa ctagcacgac agaaaatcta aatggcatca aaaataatgt gctctatgaa
    14281 gaagatcatt tccatccaaa taatttaggt tatcaattga tggcaaatgc agtgagagat
    14341 cagctgatcg agacacaaaa ccaatggtta gagaaggagg atgatcaata aacatgaaaa
    14401 caagatctga cgtatcttca aacaaaacaa agaaaacaaa ttactggaaa ttcgccttcc
    14461 ttatccttct tggggtagta cttggttcga ccgtattttt aggcagccgt atttttgcta
    14521 atcgggaacc agaactccca gaaataccag ctcttacgga aagacaagga gatcctgtct
    14581 taacgatcaa ttcaaataaa gaaaaagtca atcaaatcat cagctttttc cttagtgagt
    14641 atcaaaaaga tagtgatatt gaatataaat tttatttgga aaatgaagca ctactcaatg
    14701 gtactttcga agtactaggt tttcctatcg atttctattt atattttgat ccgtatgtga
    14761 tggaaaacgg aaatgtccaa ctaaaagcta gaagtctttc tatcggaaca ttgagtttgc
    14821 caatcaaaga tgtgatgaat atgatcaaga gaaactataa attaccagat tggatcgaag
    14881 tcaatacaga tgatctgact atcatgctta ggttggatca attccgaatg caaaacggga
    14941 tgtatattaa agcagataaa atcgacttag taaatgatga cattcggttt agtctatatt
    15001 tgccaaaaga tacctctgat gcttcaaaca actcaaatac caacaaaact agcaccagca
    15061 gtactgaaag caagacaaaa gaaaataaaa aatcatcgga tgagtagtat tagaggggga
    15121 atcataatgg aacgagcaat atttgcagga ggctgcttct ggtgtatgat ccagcctttc
    15181 gacacgcttc ctggtatcca tacagtgatg tcaggatata ctggagggca tgtaccagat
    15241 ccgacttatg aacaagtaaa aagccaaacg actggacata cagaagcagt ggagattatc
    15301 tatgatccaa cattgatttc atatgaacaa ttagtagaac tttactggca gcaaactgat
    15361 ccaactgacg ctttcggaca attcgaggat cgcggtgaca attatcgccc ggtgatcttt
    15421 tatttgacag aagaacagcg gatcattgct gaaaaaagta agaaacagtt agctgaaagt
    15481 ggacgtttcg aacagccaat cgttacaaaa atcgaaccag cccaaccatt ttatctagca
    15541 gaagaagacc atcaagagta ttataaaaag catcctgaaa attttaaaag aaaccatgca
    15601 cgccgggctg cctttatcga agaaaattgg aggaacgaag ggtgaatcga agcttttttc
    15661 attatttaat gacactgcga ggcggtggca cacatgatgc tttatctgtt ttcgctacag
    15721 aagccggtaa agacatccaa tttcctaagc acagctctag ctacgaagaa atttctaatt
    15781 atctggaact gaatgtcgac tatttgccgt ccatggatat ctttgacgaa gcatgggaaa
    15841 aatatttaga aaataatcat gacatataag agatagtttt gtactttatg atcaaaaaag
    15901 ttttttgtcc aatagcctaa ttgaaagaat tatttttatt tcagctatct agtcgcattt
    15961 ttttgatatt tttcttataa taaagaaaga atgaaaaaaa gagggatgga ttgatagaga
    16021 ttgacaaaaa aacaacgcta tattaatcgc ttctggcttg tattcaaata cttacttgtt
    16081 tctgtattcc ttgcgttcat gttgcgggga tttttattca tcccggtacc agtggaaggc
    16141 aattcaatgg aaaacgtgtt gaagcaaggt gatatggttg tgatggaaaa attttcggag
    16201 atcagacgat ttgatattgt tgtttttcaa ttagcagatg gcacgattta tatcaaacga
    16261 gtaatcggtc ttccaggaga aaatgtcagc taccaaaatg atcaattgaa gatcaatggg
    16321 aaagtagtta aggaacctta tctaactaaa aacctgaaat cagatcatgc aaatgcatcc
    16381 tatacgacgg actttacact acaagagcta acgggacaat ccaagcttcc ggaagataat
    16441 tattttgtac ttggagataa tcgaagagtt tcaaaagata gtcgttcgtt tggcacgatc
    16501 aataaaactg atattctagg taaagcaaga tttgtctatt atccacttga tgaaataaaa
    16561 tggatccagt gagaaacaat aaagcgtgca gtctgttgag actgcacgct ttattgttta
    16621 atccaaaatc agtttactac cttagtacgt aaaatcaaac agctcgttta aacacttttc
    16681 tagcctctaa agttttgata aatgaataaa caaccattat ttgaataggg atttggatca
    16741 tattcctgat gaatctacct agaaaaatta ctttgaaatc tgcaccaaac aaaacagaca
    16801 tcagccaaga attgataaaa aggctgactg ggatcataat caataattgg gcagcgatga
    16861 cggaaggcag gtttatcttt ttatgatata aaaataagcc attaataaat ccaccaagga
    16921 aagccgtgat aagaaaagca gcttgaaacg ttcctgaacc aaatatcgtg aaatttaaag
    16981 tgttcgacaa catttcaagc aaaccagccc agatcggtcc atatagataa cccataagcg
    17041 ccatacttaa aaatccgaat gtgatttgaa aataagcagg accaatcgct atcttgtata
    17101 agatgacatc aagggctaat aaaagagcag aggtcgacaa caatcttgca ttcatttcgg
    17161 gcaatctaaa aacgtctttt gcaatcaaag tgttcaaaat attctctcct ttgtcagtta
    17221 ataataaaag gataccataa aatcaccaat gtgtcatgac acattggtga tttacataca
    17281 ctggcatcta acaattaaaa aaagggtgag aagactgctt ttataaa
//
LOCUS       DACGLW010000063        17254 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.066, whole genome shotgun sequence.
ACCESSION   DACGLW010000063 DACGLW010000000
VERSION     DACGLW010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17254
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.066"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            complement(127..1146)
                     /locus_tag="IXT53_002049"
     CDS             complement(127..1146)
                     /locus_tag="IXT53_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ4905848.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(1168..2175)
                     /locus_tag="IXT53_002050"
     CDS             complement(1168..2175)
                     /locus_tag="IXT53_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HAQ4905849.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            complement(2168..2833)
                     /gene="pgmB"
                     /locus_tag="IXT53_002051"
     CDS             complement(2168..2833)
                     /gene="pgmB"
                     /locus_tag="IXT53_002051"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HAQ4905850.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            complement(2826..5120)
                     /locus_tag="IXT53_002052"
     CDS             complement(2826..5120)
                     /locus_tag="IXT53_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HAQ4905851.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            5380..7545
                     /locus_tag="IXT53_002053"
     CDS             5380..7545
                     /locus_tag="IXT53_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ4905852.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            7849..8682
                     /locus_tag="IXT53_002054"
     CDS             7849..8682
                     /locus_tag="IXT53_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HAQ4905853.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(8784..10484)
                     /locus_tag="IXT53_002055"
     CDS             complement(8784..10484)
                     /locus_tag="IXT53_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905854.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLHA
                     "
     gene            10991..12151
                     /locus_tag="IXT53_002056"
     CDS             10991..12151
                     /locus_tag="IXT53_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ4905855.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            12167..12610
                     /locus_tag="IXT53_002057"
     CDS             12167..12610
                     /locus_tag="IXT53_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ4905856.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(13093..13623)
                     /locus_tag="IXT53_002058"
     CDS             complement(13093..13623)
                     /locus_tag="IXT53_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HAQ4905857.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            13728..14279
                     /locus_tag="IXT53_002059"
     CDS             13728..14279
                     /locus_tag="IXT53_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ4905858.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(14432..14905)
                     /locus_tag="IXT53_002060"
     CDS             complement(14432..14905)
                     /locus_tag="IXT53_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ4905859.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            15043..16515
                     /locus_tag="IXT53_002061"
     CDS             15043..16515
                     /locus_tag="IXT53_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HAQ4905860.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            16512..17222
                     /locus_tag="IXT53_002062"
     CDS             16512..17222
                     /locus_tag="IXT53_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HAQ4905861.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
BASE COUNT         5360 a         3101 c         3545 g         5248 t
ORIGIN      
        1 aaagtgttct ttacttttgt cccacgctct tttttacaga taaacaatgt gttagaagta
       61 gtatcttctt ttacataaaa agctgaccat aggataaatg gtcagcgtat ttagtgagtc
      121 agctatttag tatgggtttt ctaaaggaac gactgtctct cttttgatca agcgatgagg
      181 gatgaccaaa cgtacgccac tgctcaattc ttcatggatc aattcgcgca gcttttgcat
      241 agcgacacgt cctaattcgg agatatcgat atcgatactt gtaaggtaag ggtgtgttaa
      301 tgttgagaag atcgagttgt tgaagctaat gacggagagt gtctctggaa tgacatagcc
      361 atacatttgc gccaactgca tcgtgcgaag ggcgaagata tcgtcaatga ccaccaatgc
      421 tgtggcttcg ttttctttca acgttttctc aaaggacaga taatcctccg gatgctctag
      481 tactacactg gataacgcag gaagaccagc catcatcatt gctttttgat aaccgaaata
      541 tcgttcgaaa tacaaacttt catgagtcgt gtttgtcaca aacaggatat tcttatgtcc
      601 attctggatc aaaaattctg ttgcttgttt acctagaagc tggttatcat tatctacata
      661 aacgatttcg ttctcatgat gataaggctg tccgatcaaa gtgaaaggga tgtggttttg
      721 gtataaataa tcaatgacag tatcttgact atctgaatac actagaataa aaccatctac
      781 ttgtttttgt aaatgcatac gctggacgtt ctctaacagc aattcaaaat ttttagcagt
      841 agcgattgct gtagtcatat cataattccg cgcttcttcg ttgattgctt ccatgatctc
      901 aagataaaat gggttaccga tccgctcttt tgaatcaaga gggggtaaga ttacgccgat
      961 ggcactggaa atgcgttttc ctagattgcg ggcagtcatg ttaggaacat aacctagttc
     1021 atccataatt tttcggactt tcttctttgt tgtctcggaa atactaggat gatcgttgat
     1081 gacacgagag acggtcgagg tggcaacacc ggcttttttc gccacatctt ttacggtaat
     1141 agccatgata aaaacacctt tctcttgtta ttgtgcagag aaccgataaa tcgtctgact
     1201 gatccgttct tgaccaggat ggaggttgat cgatccaaat ttggggaaat gaacgatatc
     1261 agggatttct tgcggttcga ttgctaatcc gtagtttgaa tgcatctgtt ttccattgac
     1321 agagaaatca gcttcgaatc cagtcgtaga aaataatacc atacttttat tagaagttgc
     1381 tatagtcatt tggcgaccag aatgcttctc gaataattgt aaggaagttt tggaaagcct
     1441 aggatgatgc aaaagaaata catcatctag tccatctgga taacatgcaa ggatctcttg
     1501 cagtgttggg gattttctaa agtcgattgg cagtttttct ttttttgtcc aatttccagt
     1561 aggcagtttt tcttgatcta attcaagcac accatctaaa aagaccgtta actgatgttc
     1621 atagatgtct cttgttccat ttcctgaaag gttgaagtag gcatggttgg tcggattgca
     1681 gatggtttca acaaggctac tagcagaggt tgtcatctct aattcgtttt ttgttaaacg
     1741 gtaagtgatc gtagcagtga ttggtcctgg ataggtagaa aattcatctt ttaagtaaaa
     1801 gaccacacct attgatgatg gagttttgaa aacttctact tcccaaaatt ggaaagacca
     1861 gcctttcgtg ccaccgtgga tatggtgtga tccgcagttt ttctccaatc tatgctcacc
     1921 ccaagaagca tcccggatcc ttccggctac aggtccgaca gttgctccaa aaaagctttt
     1981 gtctgttaga acatcttcga aacgatcgta agacaataaa acattttctg attttccttc
     2041 tttgtctggt gcgaagattt ggtgcaggcg ggcaccgtaa ttgagaagaa cgacttctaa
     2101 gtagtcgttc ttcaatgtga ttttctttat ttcagtctct ggaactggta cgacggagat
     2161 ctctggatta tccatggtca tgccactttt ctttcaagta atcgatcgta agaaaagtcg
     2221 tatcagggac aagcgggatc tcctctccaa ggtcttcagc acgtcccacg ccaattggtt
     2281 gagcgccact tgagaggatg gcttggatac cggcttgcgc gtcttcgatc ccgatacatt
     2341 cttctgcttt tagtccgaca gcttttgctg caaggaggaa gatatctggc gcaggttttc
     2401 cgtttgttgc ttctgccggg tcggcaatag catcaaagta aggtgtaaga cccatttttt
     2461 caagtaggaa tggcccgttt ttacttgcag aagcaagagc aatcttgatt ttttcttctt
     2521 ttaaagaatc cagtaaaggc acgaccccgg ggaagacatc ttttggttcg atcgtctgga
     2581 tcatctctaa ataataatcg ttttttcttt tcgctaattc tgcaaattct tcctttgaaa
     2641 aagagtgttc ttttccgcca taagctaaaa gcaaagagag tgagtcttct cggctgacgc
     2701 cttttagctg ttcgttgaat gtgtggtcga tcgtaatgcc gatttcctga ccaagctttc
     2761 gccaagcacg ataatggaat tcagcagtat cggtaatgac accgtctaaa tcaaacaata
     2821 cccctttaaa catttgcttc ctcctttctt agcgttattt ctagacgatc cgctaaagaa
     2881 taagactgtt catagacgta aatttctaga ggagaacctt ctaataactc gaaagtaact
     2941 tgttcatctg tgactgagat gaacaataaa cgtccgcgat aattgatatg gaaagaataa
     3001 gctttccaag tttttggcag aaatggtgcg aatctcaact gatcatgggc agttttcatt
     3061 tgagcaaatc cttggacaat cgacaaccag ctaccagtca tggaagtgat gtgcaagccg
     3121 tcttctgtgt cgttattgta attatccaaa tccaagcgag ctgttcgctg gtacatttct
     3181 acagctttgc tttccattcc taattctgct gctaaaatcg aatggatact tggcgaaagg
     3241 gaagactcat ggacagtcat tggttcatag aaatcaaagt ttttttgctt ttcttctttt
     3301 gtaaaagtat cattgaagaa ataaatcccc tgtagtacat ctgcctgctt gatgaagcaa
     3361 gatctaagga ttttgtccca agaccaatgc tgattcagag gaagatcgct tgtgggaata
     3421 tcggatacag gcatgagatc tttatctaag aatgtgtcat gctggacaaa gattcctaat
     3481 tcttcgtcaa ctgggaagta catgttctct gcgatatctt tccatttggc tagttcttct
     3541 tcgctgatag tgatagcagc ttctttttga taagttaaat aattttctcg cgtgtaacgc
     3601 aagacccaag ctgcgatcgt atttgtatac caattgttat tgatattgtt ctcatattcg
     3661 tttgggccgg tcacaccgtg gatcatgtat ttatttgctc gttttgaaaa atggacacgg
     3721 tctgcccaga agcgtgcgat ttctgctaaa acttccagtc cttcattttt caaatattcc
     3781 gtatcgccgg tgtaattcgt ataattgtag attgcatagg caatcgcgcc atttcggtga
     3841 atctcctcga atgtgatttc ccactcgtta tggcattcga caccagtgaa ggtgaccata
     3901 ggataaagcg cgccttttaa tccttgttgc tgagcgttgt ggattgcttg cggcagttga
     3961 ttatgccgat atttcagcag attcttagtt acttcaggtt tggctaaagc aagataaaga
     4021 gggacagcat acgcttctgt atcccagtaa gtggctccgc cgtatttttc tcctgtgaat
     4081 cctttggggc cgatattcaa tcgttcatct tcgccgtagt aagtagagaa tagttggaac
     4141 aaattgaatc gaataccttg ttgtgcttct tcgtctcctt cgatgaccac atcggcaagc
     4201 ttccatcgtt ctgcccaagc cgctgtttgc tcttcttttg cttggatata attgtttgtc
     4261 tggttcgtta attctgagac agctttttct gcttgctgcg cttctggaat atctctacta
     4321 gtaagaacga taatttcttt ttccactgac catgtctcac cttgagataa ttctgcagac
     4381 aagcaaccat gtaatgctaa agtatcttcg tgatagtcag ggatgactga ttttccatta
     4441 acgaaatggc gcattgctgc agtaacagta aattgttcaa tcccaaagtt gtttggaata
     4501 gttttagtcg tcaaatagcc gatttcttct atagttccac gatcaatctc attccaaaaa
     4561 tgttcatcat agttgctgtc ttcgttttga acatttccat ccaattttga tacgagtttg
     4621 actgttccgc taccttcaag catttctata gttaaataga tataagctga ttcttttttg
     4681 acaatgctca gaaaacgttc gaaggaaatg cgtactttat tagtagaagt agtgattgtg
     4741 aatgtacgag ataatacacc atttttcata tctaattccc agtaaaagtc ttctggttcc
     4801 aaagaagcca agtcgatcag ctgatcgttg atcgaaatat ctatcgcgat aaagttcaag
     4861 gcgttgatca cttttccaaa gtattctgga taaccatttt tccaccagcc gacacgtgtc
     4921 ttatcgggat accagacacc cgccaaataa gtaccttgat ggtggtcccc tgagtaagtt
     4981 tcttcaaagt tcccgcgcat ccccatataa ccgtttccaa tacttgttaa agattcttgc
     5041 agtcgaagat tttttttatc taattgtgtc gtgctgattt tccatggatc aatagcaaat
     5101 agtcgtttga tctgtttcat ttttctcctc ctaatgagct gttttatacg ttcataataa
     5161 acgcaaccga ttgcacaagt caatagacaa aagaaaatat ttcatagaaa aaagcttaaa
     5221 aaaataaatt tttaggagaa aattatagaa gggaacacac gtattatagg ttttatagaa
     5281 tggtatagac agaaaaataa aaaataaagc atttacaaaa aagaaaacgc acgctataat
     5341 acgtttaacg aaaccgattg cgtaaaggag ctaaacggaa tgaaaaagat atttagtttt
     5401 gagttttggc aaaaatttgg aaaggcttta atggttgtgg tagcagttat gccggcagct
     5461 gggttgatga tcagtatagg gaaatcaatt cccttgatca atcctgattg gacgccgctt
     5521 gtcacaacgg gcggagtaat tgaaaatatt ggttgggcaa ttattggtaa tcttcatatt
     5581 ttatttgctt tagcaatagg aggaagttgg gcaaaagaaa gagcaggcgg agcatttgca
     5641 gctgggatct catttatttt aatcaatcgg atcactgggg ctatctttgg tgtcacaagt
     5701 gacatgcttg cgaatgaaga tgcatttacc cacactctgt tcggtacgaa gatcatggtt
     5761 aaaggatttt ttacaagtgt attggaagca ccagctttga atatgggagt attcgtaggg
     5821 atcatcgctg gatttgtcgg ggcaatggct tataataagt actataatta tagaaaattg
     5881 ccggatgctt tgtcattttt caatggaaag cgctttgttc cgtttgttgt tatcttatgg
     5941 tcaacaatcg tggcattgat tcttgctgtg gtgtggccaa acgtacaggc tggaatcaat
     6001 aattttggtt tatggattgc agaatcacaa gaaagcgcac cgattctagc accgttttta
     6061 tttggaacat tagaacgttt actattgcca tttggattgc atcatatgct gacgattcct
     6121 atcaactata cacagcttgg cggatcgtat gagattttat caggcgcaca ggcggggaca
     6181 caagtatttg ggcaagatcc gttatggttg gcttgggcca ctgatttagt gaatcttaaa
     6241 ggtgcagggg atatgtcgca atatgagttt gtgttgcata actggacgcc ggctcgtttc
     6301 aaagtaggac aaatgattgg ttcttcaggt atcttgatgg gaatggcttt tgcgatgtat
     6361 cgtaatgtcg atcctgataa gaaagcaaga tataaatcaa tgtatttttc agcagcgttg
     6421 gctgtctttc taacaggtgt aacagaacca ttggagttca tgtttatgtt tgctgctgtg
     6481 ccattatatg tcatttattc agtgatccaa ggggctgcat ttgcgatggc agatatcttg
     6541 ccattgcggg tccattcatt tgggaatatc gaattgctga ctcggacacc gttagctatc
     6601 aaagcgggtc taggtggaga tcttatcaac ttcgtgttgt gtgtgatcgc ttttggtgtt
     6661 gttacgtatt tcttagcaaa tttcttgatc aaaaaattca actttgctac accgggacgt
     6721 aacggaaatt acgacaatga cagtgaagaa accgttgtat ctaacagcgg aacaggaaca
     6781 gcagatcagc aagtggtcca gatcattcat ttgctgggag gaaaagaaaa tatcaaagat
     6841 gtcgatgctt gtatgactcg gttacgcgta agtgtaaatg atcgagaaaa agtagggact
     6901 gaagaagcct ggaaacgagc aggggcgatg ggattgatcg tcaaagacaa tggagtccaa
     6961 gcagtttatg gaccaaaagc agatgtcttg aaatcagata tcgaagatct gctgcaatca
     7021 ggagcagaga ttccagagcc agaaatggtt tctacaggaa atggacaagc aggtagtaag
     7081 caatacttag gtatagaaca agaactagtt tccgcagctt ctggagaagt gattccttta
     7141 tctgaagtaa aagatccggt attctcacaa aaaatgatgg gtgacggctt tgctgttatt
     7201 ccaacatcaa gagaagttgt tgcgccgatc gctggaaaag tgacaagtat tttccctagc
     7261 aaacatgcaa ttgggctgga aacgaaagat ggtatcgaag ttttgatcca tatgggaatc
     7321 gatacagtcc aaatgaatca gccagctttt gagattgctg tcaaagaagg ccaagaagta
     7381 ggaacaggaa cgaaattagc tgagatggat cttgatgtga tccaaaacga agaaaaagat
     7441 ccaacgatca tgatcgtatt tacaaacgat aaagtagaag aagtagtgat caagcaatta
     7501 ggtacagtga cagctggtaa agtgatcggc tccattaaaa tgtaaggaga ggttgtgaaa
     7561 aagctgtcct gcatcaagtc ataagaacag ccaaacaaaa atagctcctc ctatttttcg
     7621 tttggttggg cttattaccg agaagcagca gtttgaatac tgtttagtgg agaggtcgtg
     7681 aaaaagctgc cctgcaccaa gtgataaaaa cagccaaaca aaaaatagct cctggtccat
     7741 tgagcttatt actgaggagt tagcaggaga acactatttg ctggagaggt tgtgacaaaa
     7801 attttgtcac aacccttttg tttcgaaatg aagacaagga gaaaaccaat gaaattcatg
     7861 acactcaaca cgcatagctg gcttgagcca gaaccagaga aaaaattgca agaattagca
     7921 gataagatcc tgttagaaga ttatgaagtg attgctttac aagaaatcaa ccaattactg
     7981 gaatctgaag aagtagaacc tgcgaagcta atgaagttct gtccggtaaa gaatcaagta
     8041 cctatccgta aagataattt tgcctaccgt ttagttcagc ttttaaagga acacggaaaa
     8101 gagtattttt ggagttggga aatgagccac atcggttatg ataaatatga agaaggaaat
     8161 gcactgctta cgaaaaaaac attggaaagt caggtcctta ccgtctccca atttcaaaag
     8221 aaagaagatt atcagacacg caagatcttg attgggaaaa caaagataga tgatcaggac
     8281 atttttgtct cgagctgcca cttttcttgg tggacagata aaaaaagtgg cttttatttt
     8341 gagtggaaaa acttggaaaa ctactttttg gaaactcgcg taccactttt tttcctaggt
     8401 gatttcaata atccagtaga ctctcaaggt tattatactg taagagaaag ttgccttctt
     8461 cttcaagatt cgtatgttgt cgcgaatgaa aaaggaaaag cagctactgt ggaaaagaaa
     8521 atagatggtt gggaacaaaa tacagaaaaa ttgcgtatag attttatttt cgtccctgaa
     8581 ggaatgcagg ttaaaaaata tcaacggatt tttgatggga tcgatagtcc aatcatcagt
     8641 gatcactacg gggtggaaat cgaattagat gtgaacgaat aaaaaatata acatataaca
     8701 taaaaaaact gattaaaata aaactctcca aacgattata ccgtttggag agttttttga
     8761 tcggatgtat ttatcccttt tgcctaagca tgtaaaagct tgtatctatg ataaataaat
     8821 aaacatagca aggtaaacca gccaatcaaa gtaagagaaa gaatgaaata gaaatagatt
     8881 ggcgtttgat aatgaagcgt gagctcgttg attccttttt gctgcttcac gattggggtc
     8941 ccgatatctg ttacttcata atcagtcaat gtttttccat tttgctgtag cacagtccga
     9001 tcgtagacga taacagggat atgaacagtt ttgtccccag tgtttttcca cttgattgtc
     9061 aaaagaccgc ctggatgtgt ttttataaaa gttgtttcgt tttctagtat ttcttcgtta
     9121 taccggtcgt atttattgtc ttttgtctcc ttatagattg gcagatagtc aggtgtggtt
     9181 ttttggaaaa cgaacaagct ggtagacata tctttgctga agaatgtttt ccttgcttct
     9241 tccggcgtat cgaatagata agtatgtcga ctgacaaatg tttcattttc ccacttttcc
     9301 agatgctggt ataagttctg gatcgtctgt gctgaaaaaa taagaatgcc aaatatactt
     9361 aaaagacgga accacttttg ttcagaccac tggttcagtg aaagggcaag gtaaagtaga
     9421 agcaatagcg taaatggcac gaaaaatcga aatggaaact ggatcaaact aataaaaggc
     9481 acattttttt cgatcaatgt cgtccatgga atcaagctag tagaaagaaa aaagaaaaca
     9541 agacttgtgt acaacacgat tttttggaat aaggtgttct tttgttgaaa gaaaataaaa
     9601 agcaaaaaac ctgataatat caatatagga aaggccttag ggtaatggag ccaataactg
     9661 ccatccagat ctatcgcctt ttgactcatt tctctgttaa caaagggagg aagaatctta
     9721 ttcccactgt aaacaatagc taatcctgcc caaatatttg ctgtcagaac aaaataaagc
     9781 aagaccgact taatcagttg aagcagtgct tttccttttt cagtctggtt aaaaaagtaa
     9841 taaagataaa aagggatata catcaaagct aacatgatcg aagacagtaa atggatctgt
     9901 gtcataagtc ccactgagac agcgactttg ataacaggga actttttatg aaggacgaaa
     9961 tcagcaatag ggatcataca taaaggataa aaacaagtcc cccagctagt gaatccttgg
    10021 ttgatagacc agtattgaat agaatacgta ctcatgaata aaacggctac tggtacactt
    10081 acccaatttc gaacattgac atatctcagt aaaatcagca tagaaaatcc tgaaataaga
    10141 taaagaatga aatttgaaag aatctgataa tgaaaccagc tacctgcaag caacacgatc
    10201 aatccttgta aataagcgaa aaatgggccg taaacggcat tcacgatcct gccactttgc
    10261 tggaatccat aaagggaagg aaaataatgg aaatccccat atttaatttg ttgcgcggca
    10321 tcatagaacc gattgtaatg gaatggggca tcagcaccca aaattagttt atgtgtgaat
    10381 aaatgaggca tgagaaatgc caaagcaatg aaaaaaattg ctaaaaatgg gccatatttt
    10441 ttcaattgtc gaactccttt gcaaacttta gataaattca tcatatatcc tatgcgagag
    10501 gtaggcaaga aattagaaaa ttcatgaaaa tatttatgaa ttttacaaga aaaacataag
    10561 tgaaagttct aaggagagag gatgcgacat aagttttgtt gcatctcctt gttttgaaac
    10621 agaatttttc ttaatactcg aaacaaagta gctttttcat gacctctcgg taaacggtgt
    10681 tcaatcagct gcttctcggt aataagctca ctagcctaga aaatatgaga agctattttc
    10741 tgactagcgc tcttattact caaagcataa cgcagttttc acaacctctt ccttaaacgg
    10801 tgttcaaaag cttgcatctg cggtaataag cccaacccaa caaaaaatat gagtagctat
    10861 ttttttgggc tgtacttatt acttgatgca ggacagcttt ttcacaacct catcggcttt
    10921 cattttctat ttttttcctg aacccatata ataaaagaaa gaacaaaaaa acttgcaagg
    10981 gaggacacat gtgaagcaga gtaagcaaaa acagtctcgt cgacttttgt ttcgtttgtt
    11041 tttattaata tgtatcgtcg tagggttact gatcgcttta tatccatttt atgtggattc
    11101 tttaaatagt tttattgatc aaaaacgtat cgaacaagta caaaaacgga ccgcagctga
    11161 aattgaagtg cagcgaaaga aaatggagga gaaaaaccaa cgattaactg accaaggatt
    11221 caatcctggt actgatcctt tcaatgaaaa gaacagaaat gaaagtacaa catccagcca
    11281 attagaggaa tggctgatcg gaagtatcaa tatacctaag atccaattaa atatttcact
    11341 ttatgatcgg ctcaatggta ggatccttga aaatggagca ggcgtcttgc aaggaacctc
    11401 tttcccgctt ggtggaaatt cgacgcattc agtgatctct gctcacagcg gtttacctaa
    11461 tcgacggtta tttaccgaat tagatcgttt agaaaacgga gacacgttta ttttgactgt
    11521 tttgggggag aaattggctt atcaagttaa aaatatacaa gtggtactgc cagatgacac
    11581 gtctgttcta acgatttcag aaggagaaga cttagttacc ttgcttacat gtacgccgta
    11641 tatgatcaac acgcatcgtt tgctagtgac aggacatcgg attccttata acgaatcggt
    11701 aaaaaaagaa gaaggaaaag gaaatcagga aagaaccatg cggcaatggt ttattcttgc
    11761 aggaacgata atagctgtag tcatactgtt agtgtttatc ggacgactaa tctatcagta
    11821 tagattaagt aaaaaagtgc ttgatttttc atttattatt agtgattcgt cagggaaacc
    11881 ggtaaatggt agttcattca ttttgaaaca taagaaaaaa acacttacta gaaatggtgt
    11941 gccgttttca gtacaaagcg accatcatgg gaaagtcaag ctggatcaat tgcctggtgg
    12001 aacttatcga atcgtttcag ctgatgatcc taaagttgct gtttcttttg gtattagaaa
    12061 agtaaaacaa gagaagatat acttttttga ggggcgaaaa cttgtaaaag agcttcaaaa
    12121 aaatggtttt tattttaaaa tcaatgaata agtaaggaga gaaaaaatga tccgcacaac
    12181 aacaaaattg tctgccaaag aactggaatc tgttctatcg atttggctgc atgctaactt
    12241 ggattctcat gaattcattc catcaactta ttgggagaat caagtagaat ctgttaggaa
    12301 agctttacca gatgcagtga tttacttagc agaaatagat caagaaatta ttggttttgc
    12361 tggggtgatt gatcagttta tcgcaggaat ctttgtgaaa agggaatacc ggcaacaaag
    12421 aatcggtacg caactaatga aaaaaataaa agaaaccaat aaaacactga ctttgacagt
    12481 ctatcaaaaa aatcaatcag ctattcattt ctatctttca caaggtttta caattcaaac
    12541 acagcaaaaa gatgaagcag taaatgagga ggaacttgtg atgaaatgga atcaaaacgc
    12601 tgatttttga tgaacagaat aacgaaataa tgctcccagc ctaatttttt gctgggagca
    12661 ttatttcatt ttgaagtcac taatttaact gtgtgaatat gcagttgtta caatctttga
    12721 taaacggtgt tcaatcagct gaccctcggt attagctcaa aaaacggcaa aatatgagga
    12781 actatttttc catttgtttc tctaatactc aggtcaaaac gctgatttca caacctcttg
    12841 ataaacggtg ttcaaacggc tgcttctcgg taataagccc actagcctag aaaatatgaa
    12901 aagctatttt ctaactagcg ttcttattac tcaaagcata acgctgattt cacaacctct
    12961 tgataaacgg tgttcaatca gctgaccctc ggtattagct caaaaaacgg caaaacatga
    13021 ggaactattt ttccgtttct ttctctaata ctcaggtcag aacgctgatt tcacaacctc
    13081 tctttcacaa ccttatttta ctcgtttata attttcatcg aagtattttc cgctacggat
    13141 atcatctggc attccatcgt atggttcttc gctaataaca tcttcattgt ctatacctgc
    13201 atcacccata taagtgatcg tagcaaattc aggaacaagt aattttggat aagtttcata
    13261 tttttcacga gctgcttcca tttctgcgat atgttcgttt tggaaagtaa cagtgatttc
    13321 tggatgttct tcaacccatt tagggaaaaa gatcattccg tgaagtttct tttcatttgg
    13381 cataaagtcc aatgccacgt ctgtccctgt tggctctgtc caagtgattt tgaatacgcc
    13441 gtccgtcaat ttaacgatat cagcttcttg gtcttttacc caacggcctt tgaccattcc
    13501 gccatggata cggtaatcaa cagtatggtc atttttggca taccattcat attcccaacc
    13561 attgtcatac gtatagataa agtgagttcc aataaaatct tccaattctt taaattcttt
    13621 catgtttaag catctcctta taaattaaaa catgtaataa actacatgtt ttcgattact
    13681 catttatgat agaatggatt taaagatttg tcaaggaggg aaaactgatg gcaagaaaaa
    13741 atacacttcg atatattttg ttagggttat taagtaagaa gaagatgaca ggatatgaat
    13801 tgaatcaatc gttcaaaaat gaaataggag aattttggca agcaaaacat agtcagattt
    13861 atcctgaact tgcaaaaatg gaagagcaag gaatcattca gcatcaagta gagatcacag
    13921 gtgaaaaatt ggaaaagaaa gtttatgaat taacagaaga agggaaggaa caattggcag
    13981 agtggattca tacacccacc aatgaactac cggttaatcg agacgaattt gttctgaaat
    14041 tgtatttcgt caaagacgtg aatgatccgg cattagtaga aattattcag cagcagcgtg
    14101 accttcatga agaaaaacga cttcatctat tagagcgaaa aaaaacgatc tttcctactg
    14161 aaaaagaaaa acatgaaaat tacggacact acctgatttt agcgcatgcg atcaatcggg
    14221 aaacagaata tgcaaattgg ctaacagatg tattagaaga aagtaaccga aaaaaataaa
    14281 aaaatatccg aacagtgatg gagaattcct ttctatatga aagaaacgca gatcaccgtt
    14341 cggatattct tatgttcaaa caggcctttt ttcaagtatt tatgtagcat ctacttatta
    14401 aaaagcttgg tacttaggag attagtggcg tttagagttc ttttaacaac tgttcgtagt
    14461 cagctttttc atcgacagga aggagcttgt aagttaattc gtctgtctcg ttgtcatgaa
    14521 tgatgtacat cttagcattc tcttctttga agttaccgat ttgtaaaatc gtatagcgtt
    14581 cgtcgatcaa ttcgatatct ttgattgctt ttgaaaaaac tttacggaca tggtgcattt
    14641 tcttttggcg gatttctgcc ttttctgcag cttttgacaa agtaaatccg tcatctaaat
    14701 attttttgat cagttctact tttatgactg taggtaaacg atattttcta ggacaattcg
    14761 catcagcttc gatcgaacga atgaatccct tttgttccca ataccttaat tgacgtggag
    14821 acacacctac gatatcactt aattcactga tgccaacaac caattgatca ttttccaaca
    14881 aacgtttaag ggaagcccca accatttttg tcactccttt tgtcctcttg cttcccttaa
    14941 ttatatccgt attaacttat gtgtcaagta ggttgacaaa tagcgaattt aggactaaag
    15001 tattctagta gtttcagaaa aaattcagaa agaaggagaa atatgagtaa caaccaaccc
    15061 gtagatattt acggaaaacc atataatcgg acacttttag ttgttgtctt attaattggt
    15121 acattttgta ccgttttgaa tcagacctta ttgacgacag cttttcctac attgatgaaa
    15181 gctttcgata tttcagcgtc tgatgtgcaa tggttaacga ctggtttctt gctagtcaac
    15241 gggatcatga tcccaataac agcatggctg atcaataaat tcagttcaag aaatttatat
    15301 ctttctgcca tgacgatctt tttgatcgga acgattactt gctttacagc acctaatttt
    15361 agtacattac taatcggtcg tttgatccaa gcttgcggtg tgggagtatc gatgccatta
    15421 ttgcaaaata tcatgctatc tattttcccg ccagaaaaaa gaggatcagc aatggggatg
    15481 tcagggatcg ttattggact tgctcctgct ttaggcccta cattatctgg ttatatcatt
    15541 gataactacc attggcgtga cttgtttggt atggtactgc caatcgttgt tcttgtcttg
    15601 gttttagcat tcttcctaat gaaaagtgtt attccattgt caaatcctag cattgatatt
    15661 ttatccgcta ttttatcaac gattggattc ggtagtttac tttacggatt ttctagtgta
    15721 ggagatgacg gctggggaag cttgaaagta attggtttct tagttgttgg tgtgattttc
    15781 atcgccttgt tctcatggcg tcaattacat ttggaacatc cattcttgga acttcgtgta
    15841 ttcaaatcat ctacattcac gattgcagct gttttagctg gtgtaacaaa tatggcgatg
    15901 gtaggggcag aaatggtcgt accattgtat attcaaaata ttcgtggaga atcagcattc
    15961 cattctggct tgatgttgtt gccgggtgca ttgatcatgg gggctatgat gcctgtgacg
    16021 ggtgcgattt tcgataaata cggcgcgaaa cgtttagcca tcacagggat gttcattttg
    16081 acagcggcaa ctgcaccatt tgctttcttg acaaaaacga ctccagtttt atatatcgtg
    16141 atcctttatg cgatccgtat gtttgggatc tccatggtca tgatgccggt aacgacttct
    16201 ggtatgaatg cattgccaaa taacttgatc agtcatggga cagcggtaaa taatacattc
    16261 cgtcaaatcg caagctcgat tggtacagct attttgatca gtgtgttgac gaatgtgaca
    16321 aaagcaaata tcccaagcaa atctgtattg gataatacgc cattagctta taaagatcag
    16381 gcaatcaatg ccacactgtc aggttaccat gccgcgttta tcgtagcgat catctttggg
    16441 attcttggct ttattattgc ctttttattg aagaaaaaag aacctgttct aatgaaagcg
    16501 ggggataatg catgattatt ggagttttga ttgtcagctt gcttgctttt gctttaacga
    16561 acgtgttcgc taaaaaaaca tggcaaacct ttttatcttt gattttcgga ctaatcttcg
    16621 tcgcttcact aagtttgatc gtcgctaatt tgtccaatca ctttggaatg gaaaaagtaa
    16681 cggaaacaaa aacagaaaaa attgtttcca gtgctgattc ccaaggagca gatatgcttt
    16741 tgtataaagc tttagggaat ggaaaagaaa aagtgtatct ttaccgaaca aatgaaaaac
    16801 aagaaaaacc aaaagcaact ggtacggata acgaaacaaa taaagtagaa aagacagacg
    16861 gcgatgctga aaaagtgacg aaaactactt attgggaata caaaaacgac atgtataaat
    16921 tctggttcaa tattgctgat aacaatcacg aatatgacag ccgagtgaat acattcaaaa
    16981 tcccagagac atgggtagaa ttaagtacgg accaagcagc caagttagct gaattagtga
    17041 agaaacagca aagtacgatg gaaagtgaag caaaagctta tgttcaagac ggtatggtaa
    17101 aagcaatgac tgaaaatcca aaaatgagca aagcagagca agaacaacgt acgaaagcct
    17161 tagctgctga attccaacaa caagcattcg caaaattagt gaaagaagca aaaggcgaat
    17221 aatattttga acaccatgta ggaggttgtg aaaa
//
LOCUS       DACGLW010000064        17235 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.067, whole genome shotgun sequence.
ACCESSION   DACGLW010000064 DACGLW010000000
VERSION     DACGLW010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17235
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.067"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            complement(61..663)
                     /locus_tag="IXT53_002063"
     CDS             complement(61..663)
                     /locus_tag="IXT53_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HAQ4905862.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            769..1238
                     /locus_tag="IXT53_002064"
                     /pseudo
     CDS             769..1238
                     /locus_tag="IXT53_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            1344..1928
                     /locus_tag="IXT53_002065"
     CDS             1344..1928
                     /locus_tag="IXT53_002065"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HAQ4905863.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            1931..3004
                     /gene="prfA"
                     /locus_tag="IXT53_002066"
     CDS             1931..3004
                     /gene="prfA"
                     /locus_tag="IXT53_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HAQ4905864.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            2997..3836
                     /gene="prmC"
                     /locus_tag="IXT53_002067"
     CDS             2997..3836
                     /gene="prmC"
                     /locus_tag="IXT53_002067"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HAQ4905865.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            3829..4008
                     /locus_tag="IXT53_002068"
     CDS             3829..4008
                     /locus_tag="IXT53_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905866.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            4030..5055
                     /locus_tag="IXT53_002069"
     CDS             4030..5055
                     /locus_tag="IXT53_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HAQ4905867.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            5230..6474
                     /locus_tag="IXT53_002070"
     CDS             5230..6474
                     /locus_tag="IXT53_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HAQ4905868.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            6627..7574
                     /locus_tag="IXT53_002071"
     CDS             6627..7574
                     /locus_tag="IXT53_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ4905869.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            7708..8337
                     /gene="upp"
                     /locus_tag="IXT53_002072"
     CDS             7708..8337
                     /gene="upp"
                     /locus_tag="IXT53_002072"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HAQ4905870.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(8476..8718)
                     /locus_tag="IXT53_002073"
     CDS             complement(8476..8718)
                     /locus_tag="IXT53_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905871.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            8839..8911
                     /locus_tag="IXT53_002074"
     tRNA            8839..8911
                     /locus_tag="IXT53_002074"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8872..8874,aa:Thr,seq:cgt)
     gene            complement(9030..9668)
                     /locus_tag="IXT53_002075"
     CDS             complement(9030..9668)
                     /locus_tag="IXT53_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ4905872.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(9791..10423)
                     /locus_tag="IXT53_002076"
     CDS             complement(9791..10423)
                     /locus_tag="IXT53_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAQ4905873.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            10774..11073
                     /locus_tag="IXT53_002077"
     CDS             10774..11073
                     /locus_tag="IXT53_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAQ4905874.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            11568..13733
                     /gene="acm"
                     /locus_tag="IXT53_002078"
     CDS             11568..13733
                     /gene="acm"
                     /locus_tag="IXT53_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HAQ4905875.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(14005..14490)
                     /locus_tag="IXT53_002079"
     CDS             complement(14005..14490)
                     /locus_tag="IXT53_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HAQ4905876.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     gene            14970..15611
                     /locus_tag="IXT53_002080"
     CDS             14970..15611
                     /locus_tag="IXT53_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ4905877.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            15630..16265
                     /locus_tag="IXT53_002081"
     CDS             15630..16265
                     /locus_tag="IXT53_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ4905878.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            16283..17098
                     /locus_tag="IXT53_002082"
     CDS             16283..17098
                     /locus_tag="IXT53_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ4905879.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
BASE COUNT         6002 a         2806 c         3515 g         4912 t
ORIGIN      
        1 tctttttccg aaaattcctt cttatttctt ggagttgctc actttttttg caatctcatt
       61 ttaagctttt tggctgatgg ataagaaaag cgaagcgttt ttgttcataa atagcttttc
      121 cccacgatgg aaggtatgac tagtagcaga gacaaagccc gttttttcca atcgattatt
      181 caattcttct tggctaaagc cattatggac tttcggatga ctaatttgtt cattcttgtc
      241 aaagtctacg atgatcaatt gaccgccagg agctaaaatt tcataaagtt ttgtaagaat
      301 attctccgta tcaggaatgt gtaaaagtac aagtgataga aggatgagat tagcttggac
      361 agcaggaaat tcgacagaag catcagcgtg gatcgttcgg acatttttca gatctgctgc
      421 ttggatcttt tcttctgcca ttttcagcat agtttctgaa gcatcggcaa tgatcagttc
      481 tttgaatctt tccgcaagcg gtaaactaac aagcccagtt ccgcctccgt aatccagcag
      541 gactttttgg tggctgtcgc gcggtagata agtagttagt tcttcgcgaa taatcttggc
      601 taatgcttga cggtcagggg aatcataatg gttagcgatg gaatcaaaaa tattttttgt
      661 catcaaatag ctccttacgt aaaactttga aattttaact tattatagca cgtgaaaaga
      721 aagaaagcat gctatactca attcaaaaag ggagaatcta gaaagaatat gccgatatcg
      781 tttgtaaaag atagagagga aaaaggaaag tgtgtccgag aaatattact agacttgcct
      841 gagtggtttg ggcttcctga atcaacggaa aaatatatcg aggaatcaag taaactacct
      901 ttatggtgtg aaaaaagaaa agaagaatat ttaggcttta tcactctttc acagacaagt
      961 gaagatactg ccgaaatcta ttctattgta tgggaataaa aaaagactat catcaccaag
     1021 gattaggaac aaaacttttt aaagaagcag aaggttacgc agcaaaacat tacaaatatt
     1081 tgcaagtgaa gactgtagac gaggggcact actctatata tgatcaaact atttgtttct
     1141 acgaatcatt aggattttca aggttggaag tttttccaaa tttatgggat gaatggaacc
     1201 catgtttagt tcttgtcaaa aaattggaac aaaagtgagc aatagattta atactaatct
     1261 gtgcttgatg aaaattctca ctgtaaaaaa tgctgaaaat caagtataat accataggaa
     1321 caaactggaa ggtgaaaaag agcatggcac aactattttt taagtatggc gcaatgaata
     1381 gcggcaagac gatcgagatc ctaaaagtcg cacataatta tgaagaacaa aataagccag
     1441 ttgtcttaat gacaagcggt attgatacaa gagatggttt aggcgttgtt tcaagccgaa
     1501 tcggactgaa acgtaatgcg atgccgatat ttgaagaaac agacgtattt gacgtaattc
     1561 agaaaatgga aagcaaaccc tattgtgtgc tgatcgatga agcgcaattc ttaaaaaaga
     1621 aacatgtcat tgcttttacg agaattgtgg acgaattgga tattccggtt atggcatttg
     1681 gtttgaaaaa tgattttcga aacgagttat ttgaaggatc aaaatattta ttgctttatg
     1741 cagacaaaat cgaagaaatg aaaacaatct gttggttctg ccacaaaaaa gcaatcatga
     1801 atttacatta tattgatgga aaccctgttt atgaaggcga tcaagtgcaa attggcggaa
     1861 atgaagctta ttaccccgtt tgtcgaaaac attactttca cccatcaatg attacagaag
     1921 gagaataagc atgtacgatc aattacaagc aattgaagac cgttatgaag agcttggaga
     1981 acttttaagt gacccagaag tgatttctga tacaaagcgt tttatgcagc tttcaaaaga
     2041 agaagcaaac acgcgcgaaa cagtcgaagt ttatcgtcgt tataaagaag tagtacaagg
     2101 aatcaccgat acagaagaac tgcttggtga aaaactggat gatgaaatgg ctgaactagc
     2161 aaaagaagaa ttatctgaat taaaaaaaga aaaagaagtc ttagaagaaa aaatcaagat
     2221 cttgctttta ccaaaagatc caaatgacga taaaaatatc atcatggaaa tccgtggagc
     2281 agcaggcgga gacgaagcag cattattcgc tggcgatctt ttcaatatgt accaaaagta
     2341 tgctgaagca caaggctgga aaacagaagt gatggaagct aacgttaccg gaatcggtgg
     2401 atataaagaa gtgatcatga tgatcaccgg agacaacgta tattcgaaat taaaatatga
     2461 aagtggtgcg caccgcgtcc agcgtgttcc ttcaactgaa tcgcaagggc gtatccatac
     2521 ttccaccgca acagttgtcg tgatgccaga agctgaagaa gtagagatcg atattgaaga
     2581 caaagatatc cgtacagata tctatcatgc ttcaggtgct ggcggacagc acgtcaataa
     2641 aacagcatct gctgtacgtt tgactcactt accaacaggg atcgttgttg ctatgcaaga
     2701 tgaacgttct caaatcaaga accgtgaaaa agcaatgaaa atcttgcgtg ctcgtgtgta
     2761 cgataagatc cagcaagaag cacaaagcga gtatgatgcc aatcgaaaat cagcagtcgg
     2821 aactggggat cgttcagaac ggatccggac gtataatttc ccgcaaaatc gtgtgactga
     2881 tcatcgtatc ggattaacta tccaaaaatt agatcagatt ttagcaggga aattagatga
     2941 gatcgttgat gcattgatca tgtatgatca aacatcaaaa ctggaagcta tgcaaaatgg
     3001 gtaaaatgac ctatcgagaa gtcctgtcac gggcttcttc ttttttagag gatcatggga
     3061 aagaagcata tagtatccaa tttttatttt tggaacgaaa acaatggaaa aaacttgatt
     3121 ggctccttca tatgaacgaa gagatttcag aagaagaaca gcggttgatc gaaaccgatc
     3181 tgcgacttct gcttgcagat catccacctc agtatttgct aggatatgca gatttttatg
     3241 atcatcggtt aaaggtaact gaagcaacac tgatccctcg accagaaaca gaggagttag
     3301 tcgaatggtg tttagacgag acgcctggtg taccgttaga agtgatagat attgggacag
     3361 ggactggagc aattgctatt agtttgaaag cagcaagaaa aaattggcat atatctgctg
     3421 tggatctttc agaagaagca ttagaagtag cgaaagaaaa tgctcagaaa gaaggaacga
     3481 aaatcagttt ttatcatgga gatacattag gaccagtaat ggatcagcaa tttgatgtga
     3541 ttatttctaa tccgccgtac atcagtcgga atgaatggga actgatggat gaaagtgtcc
     3601 ggtcattcga acctaaaatg gccctttttg ctgagaacga tggattagct atttatgaaa
     3661 aaatagcagt tgaagcacca agcgtactaa aatcaaatgg aaagattttt ttagaaatcg
     3721 gatttcgcca aggtgaagca gtaaaaaaaa tatttcagca agcatttcca gataaaaaag
     3781 tggcgataaa aaaggatctt tttggcaatg aacgaatgat ccaagtttat ggttgaagtt
     3841 gtggatgaaa aattttttat ttgttttttt attataaaaa aacacttatt tgacaaggat
     3901 gtaaacgact ttttggttta tcaacaagtt tatccactat tgacaaatag attatccaca
     3961 aacagtggaa aacttgagga taaactcaga caaaataaga atgtatgaaa aaggggttgt
     4021 ggataaactg tggaaacgaa acgatatgat gaaacagaat tgcaagaggc agccgaagca
     4081 ctgaaagaag gagaacttgt tgcttttcct accgaaacag tatacggatt gggcgccaac
     4141 gctcttttac caaacgctgt caagaaagtg ttttctgtaa aagggagacc gcaagataat
     4201 ccattaatcg tccatgtcgc ctcttttgaa caagtaaaag agtatgtgga caactttcat
     4261 ccggcagcag agaagattgt gaagaacttc tggcctggac cattaacttt gatatttaag
     4321 atcaaaaaag acactttgcc ctcagttgtg acgggaggat tatccacagc tgcatttcgt
     4381 ataccggata acaaaaaaac actagaagta atcgaacttt ccggtgttcc gcttgtcggt
     4441 ccaagtgcaa atacgtcggg aaaaccaagt ccaacgactg cagatcatgt atatcatgat
     4501 ctacaaggaa aaattaccgg gattattgat gatggagcta cgcgaattgg tgtagaatcc
     4561 actgttcttg atttaagtga tccaacagcc atgcctatga ttttacgacc aggtgcagta
     4621 acgaaagaac aaatcgaagc agtcatcgaa tctcctgttg ctattgatca gcatcttgta
     4681 aaagaaaatg aaacaccaaa agcccctggg atgaaataca aacactactc acccgataca
     4741 cgtgttctga tggtcagaga tggcgattgg tcaactgcag ttcaatgggc aaaaaataag
     4801 aagatccgtg caggtgtcat agcaagcccc gaaattgcgg atcaagtgcg gacagatact
     4861 gcagctgttt atatgtataa cgacaactca gtagaagcgg cggctaaagg attgtttgca
     4921 ggtctaagag gattagatga accaacactt ggattagatt tgatttttgt acaagtgtat
     4981 ccagaaactg gattggggaa tgcttacatg aatcgcttga aaaaagcagc aggacaaaat
     5041 tattttgaaa aatgatcaat tggttattgt cgcattcttt tagtatgata caatgctatc
     5101 aagtaaatct tctatcagtg aacgaaaaac taagcgtaac taatcgtaat agaagagata
     5161 cgaatatttg gcaatttgtt tttgatggga aaaataaaat tgtaggaaaa taaaaaggag
     5221 ctgttgtttg tggtagatta caaaacgttt gaccctgatc tttgggctgc gatcgccaaa
     5281 gaagaagaaa gacaagaaca taatttggag ctgattgcct ctgaaaactt tgtctccgaa
     5341 gcagtgatgg ctgcacaagg aagtatcttg acaaataaat acgcagaagg ttatccaggg
     5401 catcgctatt atggtgggtg tgaatttgtc gatatcgttg aaaatttagc gattgaccgt
     5461 gcaaaagaat tattcggagc gaaatttgcg aacgtccagc ctcactccgg ctctcaagcc
     5521 aacacggctg cttatctcgc tttggtagaa ccaggggaca cgatcttagg aatggatctt
     5581 tccgcaggcg gacatctaac acatggatca ccagtcaact tcagtggcaa aacctatcac
     5641 tttgttgctt acggtgttga tccgacaaca gaagttattg attataatgt ggtacgtatt
     5701 ttagctagaa aacatcaacc aaaattgatc gtagcaggtg ccagtgctta cggacgaacg
     5761 atcgactttg cgaaattcag agaaatcgca gatgaagtag gcgcaaaact gatggtagat
     5821 atggcacata tcgcaggatt agtagcagca ggacttcatc ctaatccagt tccttatgca
     5881 gatatcacaa cgactaccac tcataaaaca ttgcgtggcc ctcgaggcgg catgattttg
     5941 acaaacgatg aagcattagc aaaaaaaata aatagtgcgg tatttcctgg tattcaaggc
     6001 ggccctctag agcatgtgat tgcgggtaaa gcagtagcat tcaaagaagc attagatcct
     6061 gcatttaaag aatatagtga acaaatcatc gccaatgcaa aagcaatggt caaagttttc
     6121 aatcaagcaa ttggaaccag agtcatttca ggggcgacag ataatcatct gatgttgatc
     6181 gatgttcgcg agttaggtat aaatggaaaa gaagcagaga gcattttgga cagtgtgaat
     6241 atcactgtaa ataaaaactc gattcctttt gaaacactaa gtccattcaa aacaagcggt
     6301 attcgaatcg gcacgccagc aatcactact cgcgggttca aagaagaaga tgctgtaaaa
     6361 gtggctgaac tagttgtcaa agcattacaa gcaaaagatg ataacgctca actagacgaa
     6421 gtcaaaacag gtgtacgcga attaactgaa aaatttccgt tgcataaaaa ataaaaattt
     6481 aaattccatt agtaaacaga aaagttcaat gttgggcttt tctgtttatt ttttgggaaa
     6541 acggattctt tgacaatatc ggtgatgctc tctagtatat gaggtgtaat tttagtcttc
     6601 acactaaatc tcagggagga aaagtcatga ctgatcgtat ctcgttagaa aaggaagcag
     6661 tagcattcag tgatgcaaat gcgcctcatc cgcgtatcta tgaactagca ccggaagaag
     6721 gaagagtttt actagaagaa gtacaagaat cacctgtgaa caaatattcc gtcgatattg
     6781 aagacttgat ggtggatact ggcgaatggg gaaaactcaa tattcgtctc gtacgaccag
     6841 aagggaatat ggataaatta ccagttattt tttatattca tggagcaggc tgggtttttg
     6901 gcagtgctca aacccatgat aaattgatca gagaacttgc ggttcgtacg aattccgtcg
     6961 ttgtctttcc agaatatagt cgatcaccag aagcaaaata tccaacagca attgaacaaa
     7021 attatgctgt gttgcaacag ctgaataaat tagccaagtc aaaaaatctg gatctcgaga
     7081 atctaatcgt tgcaggagat tcagtaggtg gtaacatggc tacagtcatg acgatcttaa
     7141 caaaagaacg aaagggcctc ccaatatcca agcaattgct gtattatcca gtcacaaacg
     7201 ctgattttga tacagattcc tatcatgaat tcggagaaaa ctattttttg acaagagaag
     7261 gaatgatgtg gttttgggac caatatacga cgaatgaaga agagcgtgct gaaatcacag
     7321 cttcgccttt gagagcaaat ataaatgaac taaatgatct tccttcagca atgattttaa
     7381 cgggagaagc ggatgtatta agagatgaag gtgaggctta tgcgcgcaaa cttcgtgatg
     7441 caggggttcc tgttacacaa gtacgcttcc aaggaatcat ccatgatttt gtcatggtaa
     7501 atgctatgga tcagacaaag gcaacgcgtg cagcaatggc attatctact tcttggatca
     7561 agaaaaaaga ataagctttg cttgtccgca ggaaaacttc ttttcaaaaa cggattttct
     7621 taggaaaaat ggcgagtttg actagtcaat cacttgattt tgttttacaa ttaaaaagaa
     7681 acgacagaac aaaggagagc ttaaaaaatg ggcaaatttc aagtgatcga tcatccattg
     7741 attcaacaca aactaactat tattcgggac aaaaactgcg gaacaaaggt ttttcgtgaa
     7801 gtagtcgatg aaatcgctat gttgatggca tatgaagtat cacgggatat gccattggaa
     7861 gacatcgtga tcgaaacacc gatcactgaa acaacacaaa aaacgttgtc tggtaaaaaa
     7921 gtagcgatta ttccaatctt gcgcgcaggt attggaatgg ttgacggtat cttggaattg
     7981 attccagctg ctaaagtggg acatatcggt ctttatcgag atgaagaaac attggaacca
     8041 catgaatatt tcgtcaaact tccagaagac attgatgcac gtcagttgtt tgtagtagat
     8101 ccaatgttgg caacaggcgg atcagcaatc atggcaatcg atgcattgaa agaacgtggc
     8161 gcgtcgaaca ttaaatttgt ttgtttggta gcagcacctg agggagtaaa agctttgcaa
     8221 gaagcgcatc ctgatattga catctataca gcttctctag atgaaaaatt gaatgaacat
     8281 ggttatatcg ttcctggact aggggatgcg ggtgaccgtt tgtttggtac aaaataagta
     8341 aatacgaaaa agggtgtgac acaacttttg tcacatcctt ttttggtcat ttttaacgtt
     8401 tgatgcagaa cagctttttc acagctttcg tttgcatata aaaaaaccat cccaaaaatg
     8461 cagagatggt caaattcaaa tcttttttag ttctttgtag ttaacgatta ctacaccctt
     8521 actttgcgtc atcaagttcg cattttctgc agtttcatca atctcaacaa cagcggaatt
     8581 tttcaattgc tttgaaatcg ttccagtttg cgacttgccg tgaattgtga acattacgtg
     8641 agtattgatc ttgaattttt caccaatttc cgatggtacg atagttgtat caaattttcc
     8701 aaaagttgcc attttcataa aacctgcctt tcacatcaat tataccacgt tttatgaaaa
     8761 atatcatgaa attaaactaa ttttccatcg gactctttac aatcagccaa atagagtata
     8821 taatttcttt tggataaggc cagcttagct cagttggtag agcaacgcac tcgtaacgcg
     8881 taggtcgtag gttcgatccc tatagctggc attaaaagag aagacagata tatcaaggat
     8941 ttttaatcca ttgatacatc tgtctttttg tttaaaggaa ataaagtatg ctttcttagc
     9001 aatagagcat gtattatttc tataaaaatt taggagaata aaacgactag caatatagct
     9061 gtgaaatcaa atatcacatg aactgcccaa cttgtaatga tcgagttgct tttaatggca
     9121 gcttggttga ataacaatct tgccacccct tgtatcaaaa ggatgtgtgc tacagtgtgt
     9181 aaaatatttc cattatcata agtactgtaa tggataaatc caaagatgat tgctgacaag
     9241 aaactagcga caggaatgct gaacttacta gaaaaaatag cgagaaaata cactgagaac
     9301 aattcttctc caagtatcat gataggcaaa ataataaata ataacgggct gaaagcctca
     9361 tttattggat tgcctgttaa accccatttc aaaatttgtt gcaaaaatac cgagactgcg
     9421 gcactgatta ctacattgat aaaaaaatat ttggctatat ttttgacagg agcgacaggt
     9481 ttcgagaaca tttttttcgt atctttttta ccaaaaacaa gtgtaaatgc tataaatcca
     9541 gtgatagtta aagaaaaaat gctaataatg gatggcgcaa atagcaaccc taacaaaccg
     9601 agtacaacga caatagtgcc caatatccct tttttaagtt ctattttttt tcctaaaatg
     9661 ttattcatga taataactcc tactattcag tttttgaaga tagagtcagt atacatgaag
     9721 agttaaaata ggcaatgttt ttttatggat atttgtattg ctaaaaattt atcttatata
     9781 ttcattttga ttatactact tttatttgta aaaattttct agcaaaacca agtgcttgca
     9841 actgattaag cgtacaattt tttagatgtt ccgagcgtac ttcaatcatt tcaaaggagt
     9901 tgctacttaa atcgatattt tttaaatcag tatcaaaaaa gctagcattt tctaaatcat
     9961 tagtagaaaa cattatttta gtgagggaac agtctacaaa atctgcgctt gataagacag
    10021 agtgctcaaa ggtactttct tttatgtttg aaccagataa atttacatag tccattttac
    10081 aattctcgaa taacgtgttc ccaatataac tttccatcaa tttagtacct gttaatttac
    10141 aattgacaaa tttcacgcga tgcaacatgc tttttgtcaa tatacagcct gaaaaatcac
    10201 agttttcgaa tatacagtcg gtaatctcca ttctttgaaa atcattggaa gcaaaaaatg
    10261 catacttgaa aatagatttg taaataattg gatggcagat tatttggttt tcgatgacct
    10321 ctttttgtga aaggatatta tacagttcaa aatcttcgtt ttcatagata tgaaaaaagg
    10381 atcctttatt caagcttgct tcattaactt taggaaaaat catatttaat tacctccatt
    10441 ttcataaagt catattaaca aaagctaaac caacttacat tagcagaaaa tgagataggt
    10501 tttcgccttt ttcgataacg atagtgtcgc tatctcttga aacgttctta gaattgccct
    10561 gagagacaca aattaggcgt aattctaata aaaatcttag aataaaaatt tttactgatt
    10621 taatttagaa tgtttttaaa ataaaaatcg ttgccataac agtgtattga aaggttttaa
    10681 caatccagta atgatttaaa aaaatctaga tgtagcgtat actttgttta tagaatgtag
    10741 tgggaagaaa tatatttttg ggaggaaatc gtgatgtcta aaagtgaaga aatagaaaac
    10801 atggtactaa aattatatga tctcgtttta aatccagaga tcaaagaaaa ggaacgtata
    10861 cttttgatag acgcaaagac aggcttagaa aaaggtcagt attatcctaa agtcataaat
    10921 aatttggaac gtagtttacg tccgttagca atcaggggag aattgtctaa accagtatca
    10981 ccgttttata tggagatatc aacaatagga aaatttgaaa aagaattagg tcgaggaatg
    11041 gcttcggcac cgattacttt tgggcattta taaaaacgag ttattcatct caatgaatta
    11101 aaaagtatag tcaatctcat aatgcaggct aggttcattt atttgaacag agtctgtatt
    11161 tttttattct aaataaagaa acagatgttt ctttatattg tattttatcc tttaattaac
    11221 acaaaaacag ctagtataaa ctaataatgt taattaaaag tgtattttat gaaactatcg
    11281 ttatttatta atgttgcatt tttgtaagtt aaaaattgga aatatagatt gaaaaaatga
    11341 aagcaataag taaaataaaa tgaatatatg cgaaaaaata atttacatta ggaaaaacct
    11401 tgtaaaaaca gttttttggc atatttattt tttataaata tttgtaaaat aactatcaat
    11461 tttttagtta aaagattgga aatgagtggg ggaggaattg aagaaaggat ttttgagaga
    11521 tgatatagta ggttttattt ttagttaaaa taggggagat agaaaaaatg aaaaagtgta
    11581 gaaatattat tttatcaatg ttatttatta ttactaatat aacatcactg attcctgttc
    11641 atgtctatgc ggatgcaggc agagatatca gcagtaatgt cacttcgttg accgtggacc
    11701 caacgaatat tacagatgga ggaaacataa aagtaaaatt ctcgtttgac gaaaaaaaac
    11761 aaaatatcca accaggtgat tatctttgga tcaattggcc aagtgaagga aatattcgcg
    11821 gtgaaggatt ccaaaaagaa atcccattaa tgattgaaaa taaaaatgtt ggaactttaa
    11881 cggtcagaaa agattctgcg caggttgtgt ttaatgaaaa tattaaaaac ctagactcag
    11941 tagaaggttg ggggcaattt gagatacagg ccagaaacgt aaccgatacc ggtgaagaaa
    12001 atacgggacc attcaccgtg acgagcggtg ataaaacagc tactgtaaat gtgacaaagc
    12061 cagcttctgg ttcttcttct agcgtttttt attataaaac aggagacatg ctacctgaag
    12121 atactaagca cattcgatgg tttttgaata ttaacaataa cggcacttat gttgaacaac
    12181 cagttaaaat cagtgatgaa atccaaagtg gacaaagatt agatccaagt acgtttgaaa
    12241 ttaatcaaat acacttaggt gaacagaagg tttatagagg agaagaggga atccaacaat
    12301 ttttacaaga tttccccagc gctacattta atttttcagt aactgataat tacattgaaa
    12361 taactatacc aaaaaacttt gtcaatctta ggaaaataat ggtgagttat aagacgatca
    12421 tagaaaaccc agaacaaata aattttgaga atcattcgga agcttggttt aaagaattta
    12481 ataaacctgc tgttgatgga gaaagtttca atcatacagt taaaaatatt tcagcttctg
    12541 gtggagtcaa cggtacagtt agaggcgaat tgaagatttt caaatatatc aatgacacag
    12601 agattggtat cccaaatgtg actttcgaat taagacgagc agatgaacag cctattcaag
    12661 gacagtctag tatcctgttg acttcgaatg aacagggaga agctagtatc aaaggcctgc
    12721 aagtaggaga ctatgttgta aaagaaaaag aagcacccaa ttggattgat tttgatccac
    12781 taagttcaaa tgaactcaaa ttctccataa acgaaaatga tacagaggga gtcagtttac
    12841 ctatttataa caagaaaaag gttacgaaca tcacagcaac taaaatttgg aacggtggaa
    12901 ccactccaag accgagtatt tattttaaat tattcagagc aagtacaaac aattgggaac
    12961 cagttcctga tgcagaaaca aaacgattag acaatggaat aacttcagtg acttgggaag
    13021 atatccaaca gtatgatgat agtggaaacg aatatacgtt taaagtgcaa gaagtagatc
    13081 aaaacggaaa tgattatgtc ccttcaggtt atcaaaaaat tgaaaacggg ctagtcgtta
    13141 caaatgagaa taaagaagtc atttcagtct caggacaaaa aacatgggaa gacaatgaga
    13201 accaagacgg caaacgaccg acgacgatca cagtaaatct attagccgac ggtcaaccga
    13261 tccaacacaa agaagtaagt gaaaaagatg attggcgcta tcgtttcaca aatctgccaa
    13321 aatacagaga cgggcaagaa atcatctata ctgtaacgga agacaacgtg cccgaataca
    13381 gtacgacgat tgacgggtat aacatcaaaa acagttatac accggaaaaa acaaatatat
    13441 ctataccagg tagccatata acaccgtgga aaaattttcc agagaagaca gagagagaga
    13501 aaattagaaa tctattctct caaaaattgc aaatatttgc tcatacagga gaatccagcg
    13561 aacaaagaag caattatatt agtaagagag ctgtacctaa acagataaca aataaatcaa
    13621 aaagcattct tcctaaaaca agtagtaaaa aatctagttt tgctgtaatc attggtttat
    13681 tgatagtaac tatctgtgct gggatattct tacaaaaata taaaaaagtt tagttagaat
    13741 ataatatttt ttgttaaata tgactgaaat caaaagaata aaattccgat ttataatcga
    13801 aaaaagaagc atttttatat atgcttcttt tttcgtagaa tcaataaata tggatttggc
    13861 taactatgga agaatactcg tattagtttt cagcgttcat actagttgtt acattgtttg
    13921 attggccaaa agaatttctg atatattttg tgctaaataa agaaaaagac gcttgaattg
    13981 ggaaataaga aaactactct atcattacaa attttcataa actggcagat gttctaagta
    14041 aacaagtatc ttttcaaagt cggaaaaatc cataatcaac gtagtggtat tgatatttgg
    14101 atgaaagcct acggtatctt gacgatcaat agcagcatcg atcacgactt ggattttttt
    14161 ctctttgtcg tgcatcaatg ctaatggagt cactgtacct gctggaacgc ctaggaggtc
    14221 aggaagctgt tcttcagaaa caaaagataa gcgcttttct tttaattgtt cagctagttg
    14281 ttttaaatcg gcgtgctttt catcaggcaa gatgacaaga tagatttgtt ttccttttct
    14341 tgatttcaat aatagatttt ttacttgcgg accaggtagg gaaaaaggga catttttgac
    14401 tgacgtaatc gcaggatgat cgactttttg aaaagagata cccagttttt ttagtagttc
    14461 ataagcttct tgttcggttg cacgactcat cgcgcatcac cctccttttt ttattagtgt
    14521 atcataaaaa agagttgaca aatgaaaaat aatcatgtaa attacacgta aagttaaaac
    14581 aaatgacatc gaaaagaaga gtagccgtta ggtgttttta gagagtctct ggttggtgga
    14641 aagaggcagc agttaaccgt gaaacacatc ttggagtcga ttttctgaag cgtatagtag
    14701 gaaaatccgg ggagcccgtt acagctgcgg tcgttgctag atgaccagat aaggctggct
    14761 tttccgtgag gagtcggtaa aaaaaggtgg aaccacgtga ttaacgtcct ttgatcttcg
    14821 gatcaaagga ctttttgttt tcatctgaac ggcaacgact gtgtgaggtt ttttctgcaa
    14881 ggaaaaaata aacagaggtg gaaccacgta taatacgtcc tcttggcatt tgccaagagg
    14941 atttttttat tttaaaggag gaaaagaaga tggagtattt gattgaagtt atgccgcaat
    15001 tattagtagg agcactcaca acattgaaat tatttttatt taccttgatt ggctcattac
    15061 cattagggat tgttttagca ttaggattgt ctttgcattt taagccgcta cgctacttgc
    15121 ttcaaacata tgtatgggtt atgcggggaa caccgctgtt gttgcaactg atttttattt
    15181 tttatgggtt gccgacgatt gggatcgttt ttgatcgttt cgaagcagca ctcattgcct
    15241 ttatcttaaa ctatgcagct tattttgcag agatctttcg tggagggata caatcgattc
    15301 ctcaaggtca atatgaagca gcgcaagttt tgcaattaag tccctttcaa acggtgacga
    15361 aaatcgtcat cccacaagtg acaaagattg ttttaccttc tgtagggaat gaagttatca
    15421 acttggtcaa agattcttcc ttgatctata tattaggttt aggtgacgta atgcgtgctg
    15481 ggaaaatcgc gatggaacgc gacgcgactt tactgccttt gctaggcgtg gcaatcatct
    15541 atcttacatt gactggtatt ctaacaatcg taatgaaaca agttgaagga aaattaaatt
    15601 attaccgcta actgatagga ggaagaaata tgattgaatt gaagaaagtg aacaaagcat
    15661 ttggtgaaaa aaaaataatc aaagatttga atctaatcat tccagatggg aaaacattag
    15721 ctatcgtagg tccttccggt ggcggtaaaa ccacgctttt acgtatcttg gctggcttag
    15781 aaagttctga cagcggagaa ttttatttag acggtgtacc gttcaaccca ttcgagctta
    15841 aaatgaaaga acaagttgta ggagtcgtat tccaagactt ccagctattt ccacatctga
    15901 atatttttga aaacattacc ttagcaccaa aaatggtatt gaaacagaca aaagaagaat
    15961 acaccaaaaa agcagaagaa ttagcggctt cattagggat tagtgattta ttgaatatct
    16021 atccttatca gctttctggc ggacaaaaac agcgggtagc gattgccaga gcattggcta
    16081 tgaatccacg ggtattggct tatgatgaac caacaagtgc tttagatcct gaattacgcc
    16141 agcaagtcga gcgtttgatc ctatcattga aagaagatgg ggtaacccaa attattgtca
    16201 cccatgattt agtatttgca gaaaatgtcg gtgatcaatt actaaaagtt tccccaattc
    16261 attaagaaag gagaagatag agatgaaaaa aaccagtttt ctattcgctt tgatcgcagg
    16321 tcttcttctt tttgcaggct gttcgaacaa aaaaacttca gcagataatt gggcgactat
    16381 caacgaagag aaaaaaatcg ttataggttt agacgataca tttgttccta tgggatttag
    16441 agataaagac gggaagctca caggatttga tattgattta gccaaagcag tctttgaaca
    16501 atatgggatt acggtagact ttcagcccat tgactggtca atgaaggaat ttgaactgaa
    16561 caatggaacg atcgatttga tatggaacgg ctattccaaa acgccggcaa gagaaaagaa
    16621 agtgcaattt acaaaacctt atatggaaaa cgatcaagtg ctgatcacac ctaaagaatc
    16681 aggaatcaca aactttggac aaatgaataa taagagatta ggtgcgcaaa atgggtcatc
    16741 tggttatgat gtatttacga aacaaccaga agtattaaaa gagatagtca aaaaccaaga
    16801 tgctgttttg tatgatagtt ttaatgaagc attgatcgat ctgaaaagca gacgggtcga
    16861 tggtctgctg atggataaag tatacgcaga ctattatttg aaacaaagaa acgaactaga
    16921 agacttcaac ataacaaaag gttcttatga gagtgaggat tttgcagttg gtgcacgcaa
    16981 gtcagatagt acattagtga aaaaaatcaa tgaagcccta gatcaactag agaaaacagg
    17041 tgaattccaa aaaatatccg ataaatggtt tggcgaagat gttacgccga gaaattaaca
    17101 aataggggac tgtgacaaaa gacgtgtcac agtccctttt tccgaataaa cagtgttctc
    17161 ctgccaactc cttgtgactc ggagctgaat ggcaggctca caacctcttg gtaaacggtg
    17221 ttcaaaagcc agttt
//
LOCUS       DACGLW010000065        17001 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.068, whole genome shotgun sequence.
ACCESSION   DACGLW010000065 DACGLW010000000
VERSION     DACGLW010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.068"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            <1..1152
                     /locus_tag="IXT53_002083"
     CDS             <1..1152
                     /locus_tag="IXT53_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005236379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905880.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSA
                     EAPKEKVETSGSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKED
                     IENFLAGGPSSAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPT
                     RKAIAKAMVNSKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALT
                     ATVKKYPVLNASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEI
                     NEKAKLAHDGKLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIV
                     NAEGEIVVGRVMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1160..2566
                     /gene="lpdA"
                     /locus_tag="IXT53_002084"
     CDS             1160..2566
                     /gene="lpdA"
                     /locus_tag="IXT53_002084"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HAQ4905881.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            2728..3003
                     /locus_tag="IXT53_002085"
     CDS             2728..3003
                     /locus_tag="IXT53_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HAQ4905882.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            3009..3776
                     /locus_tag="IXT53_002086"
     CDS             3009..3776
                     /locus_tag="IXT53_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HAQ4905883.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            3944..5773
                     /gene="typA"
                     /locus_tag="IXT53_002087"
     CDS             3944..5773
                     /gene="typA"
                     /locus_tag="IXT53_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HAQ4905884.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            6034..7806
                     /gene="recQ"
                     /locus_tag="IXT53_002088"
     CDS             6034..7806
                     /gene="recQ"
                     /locus_tag="IXT53_002088"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HAQ4905885.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            7920..8222
                     /locus_tag="IXT53_002089"
     CDS             7920..8222
                     /locus_tag="IXT53_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HAQ4905886.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            8225..9388
                     /locus_tag="IXT53_002090"
     CDS             8225..9388
                     /locus_tag="IXT53_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ4905887.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            9414..12842
                     /locus_tag="IXT53_002091"
     CDS             9414..12842
                     /locus_tag="IXT53_002091"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HAQ4905888.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            12863..13987
                     /locus_tag="IXT53_002092"
     CDS             12863..13987
                     /locus_tag="IXT53_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905889.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            13977..14414
                     /locus_tag="IXT53_002093"
     CDS             13977..14414
                     /locus_tag="IXT53_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HAQ4905890.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            14398..14736
                     /locus_tag="IXT53_002094"
     CDS             14398..14736
                     /locus_tag="IXT53_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HAQ4905891.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            14855..15415
                     /gene="rsmD"
                     /locus_tag="IXT53_002095"
     CDS             14855..15415
                     /gene="rsmD"
                     /locus_tag="IXT53_002095"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HAQ4905892.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            15412..15903
                     /gene="coaD"
                     /locus_tag="IXT53_002096"
     CDS             15412..15903
                     /gene="coaD"
                     /locus_tag="IXT53_002096"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HAQ4905893.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            15893..16936
                     /locus_tag="IXT53_002097"
     CDS             15893..16936
                     /locus_tag="IXT53_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAQ4905894.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
BASE COUNT         5757 a         2945 c         3750 g         4549 t
ORIGIN      
        1 ccgtcacctg tcacagggac agttaaaaac gtgattgttc cagaaggcac tgtagcaaat
       61 gttggggatg tattagtaga aatcgatgct cctggacaca atagtgcacc gtcaactagt
      121 gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat caggttcagc aagtgtcgtt
      181 gaagctgccg atccaaataa acgtgtattg gctatgccat ctgttcgtca atttgcccgt
      241 gaaaaagatg tagatatctc acaagtaact gcaactggaa aaggtggcag agtaacaaaa
      301 gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg caccagcgaa atcagaagcg
      361 ccagaagcgg cagcaccgaa agaagcagct cctgcagcag aaagcaaacc agctgcacca
      421 gcaaaaccat tcaagtccaa cttaggagac ttggaagaac gtgtagcaat gacgccaaca
      481 cgtaaagcta tcgctaaagc aatggtcaac agtaagcata cagctccaca cgtgacattg
      541 catgatgaag tagaagtgtc taaactatgg gataatcgga aacgatttaa agaagttgct
      601 gctgcaaatg gtacgaaact tactttcttg ccatatgtcg tgaaagcttt aacagcgact
      661 gttaagaaat acccagtatt gaatgcatct atcgacgatg caaaccaaga aatcgtttat
      721 aaacattact acaatatcgg tattgcaact gatacggatc acggactata tgtaccaaat
      781 gtgaaagatg cagaccgcaa aggaatgttt gctattgcag acgaaatcaa tgagaaagca
      841 aaattagctc atgacggcaa actatctgca gaagatatgc gcaatggaac aattacgatc
      901 agcaatatcg gatctgtagg tggcggttgg tttacaccag tcatcaacta tccagaagta
      961 gctattttag gtgtaggaac gattgcacaa caaccaatcg taaatgctga aggcgaaatc
     1021 gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc accgtatcgt agatggagca
     1081 actgcgcaac aagcaatgaa taatatcaaa cgtcttttag ctgatccaga gctattaatg
     1141 atggaaggat gataaacaaa tggtagttgg agattttgct gtagaattag atacagttgt
     1201 aattggtgcg ggacctggtg gctatgtagc tgccatccgc gctgctgaaa tgggacaaaa
     1261 agtagcaatc attgaaagag agtatattgg cggtgtttgt ttgaatgtcg gctgtattcc
     1321 ttcaaaagca ctgatagctg caggacacca ttatcaagag tcactagatt ctgaaatgtt
     1381 tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa acacaagaat ggaaagaaaa
     1441 caaagttgtc catacgttga cttctggtgt tggtttccta ttaaaaaaac ataaggttga
     1501 aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca ctacgcgtca ttcatccaga
     1561 ttcagctcaa acttattcgt tcaataacgc aatcatcgca acaggttccc gtccaatcga
     1621 gattccagga tttaaattcg gaggacgtgt gttagattct actggcggat tagcattgaa
     1681 agaagtacct aagaaatttg tgatcatcgg tggcggtgtg attggtgccg aattgggcgg
     1741 agcgtacgca aacttaggag cagaagtaac gattcttgaa ggctcaccac aaattttacc
     1801 aacttacgaa aaagatctag taaaattagt agaagatgat ttcaagaaaa aaggcgtaac
     1861 agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac ggtgacagcg tgacagtgaa
     1921 atatgcagtg gacgggaaag aagaatccgt tactgctgat tatgtgatgg tcactgtagg
     1981 tcgtcgtccg aatacagatg atatgggctt agaacaagcg ggtgtcgaag taggagaacg
     2041 aggattgatc acggtggata aacaaggacg tacgaacgta cctaacatct atgccatcgg
     2101 tgatatcgtt ccaggggcgg cattagcaca caaagccagt tatgaagcaa aaatcgctgc
     2161 tgaagcaatc tctggcaaaa aagtagctgt agattataaa gcaatgccgg cagtagcctt
     2221 cactgatcct gaattggctt ctgtaggtat gacgatcaaa gaagcaaaag atgctggtat
     2281 cgaagcaaaa gcatataaat tcccattttc agggaacggt cgggcattat cgcttggtaa
     2341 aacagaagga ttcatccgtc tagttacgac gatcgaagat aacgtgctta ttggcgcgca
     2401 aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta gctttagcaa tcgaatcagg
     2461 aatgaatgct gaagatatcg ccttgacgat tcatcctcat ccatctcttg gcgaaatcgt
     2521 catggatgct tcggaattag cattaggttt accaatccat atttgatttg aataatacag
     2581 gcgaaacgca actgaaaaat cagttgcgtt ttgtttttta gaattatcaa taatattatt
     2641 atgtaaactt aaaaatatga caacttcttg gtttattacc acttcctatg ctacactgaa
     2701 gaggaatatg aaataggaga gttgaagatg caagaatatc agtatccact tgatttagat
     2761 tggacaacag acgaaatggt cattgtaatg aagatgtggg aagatctgga acgtgcgaat
     2821 gaaaaaggga taaacaatga agcttttttg aagacttatc agcagtttaa aactgttgtc
     2881 aaatccattg gtgaggaaaa acgattggga cgggaatttg aaaaagcttc cggttattct
     2941 ttatatcgta cagtgcagca agcaaaaaaa aatccaaaca aaactttgag aatgaacggg
     3001 tgagataaat ggaacagatg attgatcata tcaagtcctg gcttgaagaa gctggtgaga
     3061 aaatcaaatc ttctcaacaa acattaacaa tctcgcaaaa atcaaatcga acggatctag
     3121 taacaaatgt cgatcgaaac atccagaatt ttctaatcga aaaaatccat tcctttgatc
     3181 cagaggcgaa aatattggca gaagaagaag ggtgtaatca attatcggat ctcgatggaa
     3241 gagtctttat catcgatcct attgatggca ccttgaattt tgtgattgaa ggggaaaatt
     3301 tttgtattat gctcgctgtt tatgaatccg gcgttggaaa gcttggtttc atttatgacg
     3361 tgatgagaaa tgaacttttt tggggtggaa aaggaatagg cgtgtataaa aatgatgagc
     3421 cattgcctag accagcaaat aaatccttat cggaaggctt acttggtgtg aatagttatc
     3481 ttttcggtca taataaatat ggaatccgag aaattgcgga aagcagcatg gggacacgta
     3541 tgtacggatg cgctggtctt gaactagtgg caatcttgaa aggaaaccac attgcttata
     3601 tttcaaacct tagcccttgg gattatgcag cgggaaatgt cttattggaa gagtttggta
     3661 tggtaacaag cggtcttttt ggtactcctt tgacatttga aaaaagggaa tactatttag
     3721 cagggacttc ttgtgcatat gcagaaatcg cagacctttt gaaaaaagaa ggctgaaaaa
     3781 aaggtagaaa tcattcacaa aaatgataaa aacgagcaaa agattaattt tctcgaaaaa
     3841 atgaaaacat tttactatat ttcatgaaaa aagtctgcta taattagcag tcgagtaaaa
     3901 tttgccctgt cataaagaac caataaaaaa ggagttagtt acattgaatt atagaaacga
     3961 tatccgtaac gtcgcaatta ttgcccacgt tgaccacgga aaaacaacat tagttgatga
     4021 actattaaaa caatcagata cattagacgc acatacgcaa ttgcaagaac gtgcgatgga
     4081 ctcaaatgcg ctagaaaaag aacgtggtat cacgatttta gccaaaaaca cagctgtaga
     4141 ctataaaggt attcgcgtaa acatcatgga tactccagga cacgcggact tcggtggcga
     4201 agtagaacgg atcatgaaaa tggttgacgg cgttgtgttg gtcgtagatg cgtatgaagg
     4261 aacaatgcct caaacacgtt ttgtattgaa aaaagcactg gaacaacata tcacaccaat
     4321 cgttgtcgta aacaagattg acaaaccttc tgctcgtcct gagcatgtcg tagatgaagt
     4381 tcttgaacta ttcatcgaat taggagcaga tgatgatcag ttagactttc ctgtgattta
     4441 tgcatctgca ctaaatggta cgtcaagctt gtcagatgat ccagcagatc aagaaccaac
     4501 catggcacct atttttgata caattattga aaaaatccct gctcctgtcg ataacagcga
     4561 cgaaccatta caattccaag tttcattact tgactacaac gattatgtag gtcgtatcgg
     4621 aatcggacgt gtatttagag gaacaatcaa agtaggagac caagtagcat taattaaatt
     4681 agacggaaca gttaagaaat tccgtgtaac gaaattattt ggtttctttg gactaaaacg
     4741 tctagagatt caagaagcaa aagctggaga cttgattgct gtctcaggta tggaagatat
     4801 ctttgtcggt gaaacagtta ctcctgtgga tcatcaagat gcgttaccta tccttcacat
     4861 tgacgaacca acattgcaaa tgactttctt agttaataat tcaccatttg ccggtcgaga
     4921 aggtaaattc gtaaccgctc gtaagattga agaacgtttg atggcagaac ttcaaacaga
     4981 tgtatcctta cgagttgaac caacaaactc tccagatgca tggacagttt ctggtcgtgg
     5041 agaattgcat ttgtctatct tgattgaaaa catgcgccgt gagggatacg aattgcaagt
     5101 atctcgtcca gaagttattg aaaaagaaat cgacggtgta aaatgcgaac cgtttgaacg
     5161 cgtgcaaatc gatacaccag aagaatatat gggtagtgtt atcgagtcct tgagcttacg
     5221 taaaggtgaa atgcaagata tggtccatac aggaaacgga caaatccgtt tgacattctt
     5281 aacacctgcc cgcggtttga ttggttattc aactgaattc ctttcaatga cacgtggtta
     5341 cggaatcatg aaccatactt ttgaccaata tctgccaatg cttcctggtc aaattggcgg
     5401 acgtcaccaa ggggcgcttg tttcgatcga tacaggaaaa gcaacgactt attctatcat
     5461 gagtatcgaa gaacgcggta cagtcttcgt agaaccaggt acagaagtat atgaaggaat
     5521 gattatcggg gaaaacagcc gtgataatga tttgactgtc aacatcacga aagcaaaaca
     5581 aatgacaaac gtacggtctg ctactaaaga ccaaacatct gtcatcaaaa aaccaaaaca
     5641 attgacgttg gaagaatctt tggaattctt gaatgatgat gaatattgcg aagtaacacc
     5701 agaaagcatt cgcttgagaa aacaaatttt agagaaaaat gctcgtgaaa aagcatctaa
     5761 aaagaaaaaa taatctgtaa aaggatgaag agagaaacca gcttgttata aaaaatagct
     5821 ggtttctttt tttgtccaac tcttttctgt ggagaagtca tctttgtcaa tggacataaa
     5881 ttcagaaaat acgccgaaag acgtttaaaa gacttttaaa aaacttattt ctagagagtt
     5941 tttgtttcat aataagataa tgtttcacga aaggagatca acttgtcctt cgaagaaaat
     6001 cgaagtaaaa tggcaagaga aaggagcggg aagatgaacg acttattaaa acaatacttt
     6061 ggctatgatg agtttagacc tggtcaaaaa gaaattatcc agaaagtcat tgatcaggaa
     6121 aatgtattag gaatcatgcc taccggaagt gggaaatcaa tctgttacca gcttccagct
     6181 ttactattag atgggcttac tgtggttgtg tcccctttaa tctctttgat gaaggatcag
     6241 gtggacgcgg cgaatcaatt aggtataccc gccactttta tcaatagctc gttagatggt
     6301 tacgaaacag ctagacgctt ccaagaaatc gatagacagc agtaccgttt actttatatc
     6361 gctcctgaac gttttatcat gccggatttt atccaagcaa tgaagcgatg gaacgtccgt
     6421 atgatcgcta ttgacgaggc acattgtatc tctcagtggg ggcatgattt ccgcccaagt
     6481 tatttacaga tggcgaacca gctagatcaa ctgccaaatc gtcctgtaat tgttgctttg
     6541 acagctacag cgactgtgca agtagctgct gacataaaaa gacttttaaa aattccagaa
     6601 aacaaccata ttcagactgg ttttgaacga gaaaatctac gatttcaagt aattaaagat
     6661 caaaaaaaag aacaatatct aatcgaatat ttaaagatca acaaaaatca atctggtatc
     6721 atttatgcag caacgagaaa agaagtagac cgtctctatc atttgcttaa aaaattcgat
     6781 ttttccgtgg gtagatatca tggtggtcta aatgagaatg agcgaacaga aatgcaagaa
     6841 gcctttttat acgatcgctt gcagctgatt gtagcgacaa atgcatttgg tatgggtata
     6901 aataaaagca atgtccgttt tgttatccat tatcaaattc ctggctctct agaagcatac
     6961 tatcaggaag ctggtcgggc gggaagagat ggtctttcca gtgaagccat cctgcttttc
     7021 tctcctcaag atatccaagt ccaaaagttt tttatccagc agtcccaaag agaagaaggg
     7081 caaaagcaaa aagaatatga aaaattaaaa gcgatgacag aatacgtgta tattgagtcc
     7141 tgtcttcagc aatacatcct aaactatttt ggcgagacaa gcagtccgtg caaccgttgt
     7201 ggtaactgtt tagatgacag agaaatagta gaagtaacga cagaagcaca gatggttctt
     7261 tcttgcttaa aacgtatggg tgagaactat ggtaaacaaa tgctgatgaa agtcttagca
     7321 ggatcgaaag aacaaaagct gcaagcttta ggttttggac atttatctac ctatggactg
     7381 atgaaaaacc aatcgcaaaa agaaacgatg cagttaattg aatatttgat atcgaatggc
     7441 tatcttctga ccatcaatgg tgagtatcct gttttgaaag taacagaaag agggatacaa
     7501 gtattgaaag gacaagaatc tgtttatcga aaggaaccca agaaagtcca gcagctatcg
     7561 gatgaagaaa ccgatacact atttgaagtg ctgcgggaac tgcgaacgga tctagcatca
     7621 gaagcaggcg ttcccccgta tgtcgtattt tcagattcca cattgaaaga aatgagccga
     7681 atacgtccat caagcagatt ggagatgctt cagataaaag gagttggcca aagcaagctt
     7741 gataaatatg gcgaagcctt tttatctcgg ataaaaaatg cggactctaa cgttctggat
     7801 aagtagaaag aacgaatacg taaaaatgaa taaaaacaac tggttaaatc tgtttttttt
     7861 gctccttatg aaaatcaaat atgttataat taaggaatta gaatgggagg taagcacaga
     7921 tggattcttt gtcgaaaaaa cttgcgatcc aagtattaaa tgatgaagca gaacggatca
     7981 aacgattaat ccgaaatcaa aacaacagtc tctgtatctc acaatgcaaa gccttcgaag
     8041 aagtggtaga tacacagatg tacggctttt cccggcaagt cggttttgct gtccgtatag
     8101 gagtaatcgg aaagaacgag ggccagctga tgttaagtga gctagaacga gaattgaatc
     8161 agttatatag tgaggtttac caagaaattt atgataaaaa agaagttggc aaggaggaat
     8221 agacttgcct aagaaagtga aaaaaaggca tctattagac tatagcatct tgattcccta
     8281 tttaatttta tgtgtattgg gattgatcat ggtatatagt tctacgtcgt atctattatt
     8341 agaaaacgga caaaatccgt ccgcatcagt cattaaccag agtatttttt gggtgcttag
     8401 cttgattgct atcgcactac tttataaaat gaaaacagat gttttgaaaa accagcgttt
     8461 gatcatggca gccatcgcag ttttgacgat tttgctgctg attgttgtgt tttttggtaa
     8521 ggagatcaat ggggcgaaag gttggctgca aattgcagga ttctcgatcc aacctgccga
     8581 atacctaaaa atcatttcta tctggtacct gtctctgact ttatctaaaa gacaaaacag
     8641 tgtacaaaaa gattttcttg ggactgtgaa acgtccactt gcaatggtta tcggacttac
     8701 tgcattagtc gctattcttc ccgattttgg taatgcggca gttatctttt tgattatttt
     8761 ggttctgctt ttagccagcg gagtcaatta tgtgtatact ttgattgtag gagtaggagg
     8821 cttttgtcta agtactttta cgatctggct gatcaatatc acaaacggaa agatcttacc
     8881 cggacgttta cagtatatct ataatcgatt tgctatttac caaaatcctt tctcagacga
     8941 attaaataaa gggcatcaac tagtcaacgg ctactatgcc atgttcaacg gaggactatt
     9001 tggcagggga ttaggtaaca gtatccagaa aaaaggattt ttacaagaag cacaaaccga
     9061 ttttatttat gcaatcgtag tggaagaact gggagtgatt atggggattt tgatattagc
     9121 gttgctattc tttatgattg tgcgcattgt ccttgtaggt atccgttcaa aagatccgtt
     9181 caattcgctt ttgtgcattg ggatcggcgc aatgtttttg atccaagtat ttgtcaatct
     9241 tggaggaatt actggcgtta ttcctttgac agggatcacg ttcccatttt taagtcaagg
     9301 aggctccagt ttgctgatgc tttcgatttg tgtcggcttt gtcctcaaca tcagtgcgga
     9361 tgaaaaacgc aagagcttag gtttataata aaaaaagatg taggagtggt tcgatgaaaa
     9421 aagttttagt tgctaacagg ggtgaaatcg ctgtacgtgt atttcgtgct tgtacagaat
     9481 taggaattaa aactgttgga atctatgcag aagaagatga atattccgta caccgtttca
     9541 aagcggatga agcatattta gtaggacaag gaaaaaaacc gattgatgcg tatctagata
     9601 ttgaagggat catttctatc gcgaaagaat gcggagctga cgctattcat ccgggttacg
     9661 gtcttttgtc ggaaaacttg aattttgcgc aacggtgcga agaagaaggc atcatttttg
     9721 ttggacccaa actgcatcat ttagatattt ttggagataa aatcaaagca aaagctgcag
     9781 ctatcgaagc ggggattgct tcgattccag gcacagatgg accaatcgct tctatagacg
     9841 atgccttgga gtttgcgaaa caatacggtt atccgattat gatcaaggca gctctaggcg
     9901 gcggcggacg cggcatgcgt gtagctcacg atgaaaagag tgcaagagag ggctacgaac
     9961 gtgcgaaaag cgaagcaaaa gcagcctttg gtagtgatga agtgtatgtt gaaaaataca
    10021 tcgctaatcc taagcatatc gaagtacaga ttctcgggga tacgcatgga aacgtcatcc
    10081 atctttttga aagagattgt tctgttcagc gccgtcacca aaaagtagta gaagtagcgc
    10141 cttgtgtctc tatgaatgaa cagcaacgtc aaaaaatctg ccaggcagct gtccaattga
    10201 tgaaatatgt cgggtatgtc aacgcaggta cagtagaatt tcttgttgaa ggagatgatt
    10261 tttactttat cgaagtcaac cctcgcgtcc aagtggaaca tacgatcaca gaaatgatta
    10321 cagatattga tatcgtaaca acgcaactgt tgattgctca aggtttggat ctgcataaag
    10381 aaatcggatt gcctcagcaa gaaggaatta aattaaatgg ttcagccatt caatgccgaa
    10441 tcactacaga agacccgttg aataactttt taccagacac agggaaaatc gatacgtatc
    10501 gttcacctgg tggttttggc gtacgtctgg atgtcggaaa tgcttacgcg ggctatgtag
    10561 tgacaccgta ttttgattca ttgctagtaa aagtatgtac gcatggtgct acctttgaaa
    10621 cagccattca gaaaatggaa cgttgcttga aagaatttag aatccgtgga gtgaaaacaa
    10681 acattccatt tatgctcaat gtcatcacac atcctgaatt ccaatcagga aatgcaaaaa
    10741 caaccttcat tgatagtaca gcaacattat ttgaatttcc tcgactacga gaccgcggaa
    10801 acaaaacgat gaaatatatc ggtgaaatta ctgtgaatgg ttttccaggt atcgaaagtg
    10861 gcgaaaaacc attttatgaa gaaccacgta tgcctaaaga tctaatcaca agaactgatt
    10921 acgtaacggc taagaatgta ttagatgcga agggtgccga tgctttggtc gaatggatca
    10981 aaggacagga aaatttgttg ttgacggata cgacatttag agacgcccat caaagtctgc
    11041 tagcaactcg tgttcggaca aaggatttca agcagattgc acgtttgact ggagaaggtc
    11101 ttcctgaact cttttcaagt gagatgtggg ggggagcaac atttgatgtg gcttaccgct
    11161 ttttgaatga agatccttgg caacggttga gaaaaattcg ttctttaatg cctaatacgt
    11221 tgctgcaaat gcttttcaga ggttcaaatg ccgttgggta ttctaattat cctgacaatg
    11281 tattggtgga atttgtcaaa gaagcagcag cgcaaggaat cgatgtattt cgaatctttg
    11341 atagtttgaa ctggactcca caaatggaaa aaagtatcca agctgtacgg gatactggta
    11401 aaatcgcaga ggcagctatt tgctatactg gcgatatcaa cgatccaagt cgggcaaaat
    11461 acaatgttca gtattataaa gacatggcaa aggaactaga acaactgggt gcgcatatca
    11521 ttgcaatcaa agatatggct gggctgctta aaccgcaggc agcttatcgt ttgattagtg
    11581 aattgaaaga aacgacagat ctgccaatcc atctgcatac tcatgatacc agcgggaatg
    11641 gaatcatcac ttattctgct gcttcaaaag caggagtaga catcgttgac gtggcaatga
    11701 gtgcaatgag tgggaatact agtcagccta gcatgagcag cttgtattat gcactggtca
    11761 atggtccacg gctgccagaa atcacgattg aaaatgcaca gaaattaaac cattattggg
    11821 aagatgtgcg catgtattac aaaccgtttg aaaacggctt gaatgctcct gaaacagaag
    11881 tatatatgca tgaaatgcca ggaggacagt attcgaatct acagcaacag gcaaaagctg
    11941 tcggattagg tcatcgctgg gatgaaatca aacaaatgta tcatactgtc aacttgatgt
    12001 ttggcgatat cgttaaagta actccatcat ctaaagtagt tggagatatg gcactattta
    12061 tggtccaaaa tgatcttaca gaagaagata tttatgaaaa aggcgaaaca ctaagtttcc
    12121 ctgaatctgt tgtgactttc ttccaaggag aactagggca acccgttggc ggatttccta
    12181 aaaaattgca aaagattatt cttaaaggca gacctgcatt gaatgaacgt ccaggtcttt
    12241 tggctgaatc agtcgacttc aacgaagtaa aaaaagaatt ggcagaaaaa attggctatg
    12301 aaccaaaaca agacgaagta ttaagctatt tgatgtatcc gcaagtcttt ttggactatc
    12361 aaaaagcata taatcaattt ggcgatgtta cattactgga cacaccaaca tttttccaag
    12421 gtatacgttt gggtgaaaca atcaatgtac aaatcgaacg cggaaaaacg cttatcattc
    12481 gtttagatga aatcggagaa cccgatatcg aagggaatcg cgtactgttc ttcaatttga
    12541 atggacaaag acgtgaaatc actatcaaag ataattcgat catcagtacg gtccaaaccc
    12601 gattgaaagc tgaaccgaca aaccgagaac aaattggagc aacgatgtct ggttctgttt
    12661 tggatgtatt agtgaaaaaa ggcgataacg ttaaaaaagg tgatacgttg atgattactg
    12721 aagcgatgaa aatggaaacg gccatcgaag cacgttttga cggagaagtc gcacatgtat
    12781 atgtgtcttc aggagacact atttcgtctg gtgacctttt gattgaagtg actgagaaat
    12841 aataagcgaa aggtggggaa acatgaaaag actgggtttg ttttggctga ttctgctatt
    12901 agttattgtg gcattctaca tggaaccagt cttgtttccg ccaaaacttg aagtgaagga
    12961 aaactctgat caacaaatat caaaccaaat gacaacgtgg aaataccagg agcttaaagc
    13021 aactggtttt tctacttata tcggaaaacc gatcgatcag ctggaaaagg attttggcca
    13081 agcctatgat cgattaacta gcggatttgg ttttgaaaca agatattatg aaaataaagc
    13141 tagtcatctc acatttgaag cgaacatcca agacagcaga gtcacaactg ttaaagtact
    13201 gcaaacaggg cctgctgata tcgcgccgtt ttatttaggg atgacgatgc aagatcttac
    13261 gaaaatcact actatctata ctaatttcac ttttgagtac catgatacag aagttggaat
    13321 cgaactaatg gaagaggata tgaattatcg tccgctaatt gcttttgata acgacacttt
    13381 tgctatttta ttttttgatc aaacaacaaa cggcctattt tcagttgtgt atttagataa
    13441 agatagtctt ttgaaactat tgccatatca agtgtttggt gaaggccttc cacattatca
    13501 agcagataaa tcggctgatt gggaatcaat caatcgcgtt aaagaacgaa aaagtacgac
    13561 tttattaaat atgcttagaa gagaagacga acttcccata tacaatcaaa cacttgcctt
    13621 tcaaaaccac agtcagctgc tactacatga ttttttgaca aggcctgagg aaatattgtc
    13681 agaggaacgt atggctgatt ggcaaaaatc gctaggcagt gctcaaacga cagtgaaatt
    13741 ctcactgacg aatgatgaat tggagaagtt aatcagtaaa caaaaattag aaaatactag
    13801 tgggctattt attcatccgg cgattgacgc tactttttcg tttttatatt ggtacagcga
    13861 tccttacaac catgaccgtt tcatggaccc gggaacggat tctgtaggca ttgctttttc
    13921 taaagaaaat atgttagtct tattgcaaga agaaaacgga gaaagtagtg atagcagtga
    13981 tcgttaacga agagctgttt caactggagg atcaatgcct ccaagtcgct gaaatgataa
    14041 aaaagagtaa aacgatggct tcttatcttg aacataaaca gtcaatggaa gaatctgcaa
    14101 aagtttgcca gttgaaagaa gcgttcttgt ctaagaaaga agcatttgaa aatgtagcgg
    14161 catatggtga gtatgcacct gattatcgtg aaaaaaaacg agctgttcga aaagcaaaaa
    14221 gacagcttga tatggatgag catgttgcag ctttccgata tagtgaaaca cagctgcaaa
    14281 gtctgttgga tcaaattgtc caagtacttg ccgaagaagt ttctaatgaa atcaaagtag
    14341 atgcaggtag cccgtttttt gaaacgaaaa aacactttgg atgcggaggt aattgtcatg
    14401 caattagaca atagtgaatt tgaaataaca aaacgacgcg ggctgatcgt atgggtctac
    14461 agcttaaaac aattaaagaa tctgaaaaag ttcggcttgt tgcattatgt ctcaagaaga
    14521 atgaaatatg tcgtgcttta cttaaatgaa gagacattgg aagaaacaga agaacgaatc
    14581 cgtaaattac attttgttcg aaacgtagaa cgttcttacc gaccggatat cgaaatgaat
    14641 tttgccgaaa aaataggaaa caaaacagta ttaaaagacg aaggtttcga aattgaagag
    14701 ctaaatacag aaatccgatt agctgaacat ctttaaaaat ctcttatgag cagtttttaa
    14761 ataaaaaata acttggataa cgatcgattt ttgatcattg tccaagtatt tttttggtat
    14821 actagtacca gtaccatttc taaggagtga gtagatgcgg ataatatcag gaaattatgg
    14881 cgggcgccgt ttaaaaagtt tagcaggggc gaacaccaga ccaactacag ataaagtaaa
    14941 agaatccata ttcaatatga ttggaccata ttttgatgga gagacagtat tggatctgtt
    15001 tgcaggaagc ggtggtttag ctatcgaagc agtatctaga ggctgcagtc atgcggtatg
    15061 cgtagacaaa aattaccaag cgctaaaaat catcaaagaa aacatcgaaa ttacgaaaga
    15121 accagagaaa tttaccacaa taaaacttga tgcggacaaa gcaatcgaac ggctagcagc
    15181 ggagaaacaa caattcgact acttgtttct tgatccgcca tatgcaaaac aaaaaataat
    15241 cgatcaaatc cataaaatgg aagaactggg acttttttct aaaaccgcag ttatcgtatg
    15301 cgaaacggat aaaacagtcg aattgccaga aactgtggct ggattccatc aaataagaaa
    15361 acagatttat ggcataacgg ccgtaactat ctatagaaaa gagggaaatc aatgaaacgt
    15421 gcattattcc caggaagttt tgatcctttt acaaaaggtc acttagatac ggtggaacgt
    15481 gcggctaaat tattcgatga ggtagtaatt ggtgttttta tcaatacttc aaagaaaagt
    15541 ttgtttcccc cagaagaaag aatgacattg atcacaaaag ctgtttctca tcttcctaat
    15601 gtaaaagtca tgcatcaaga aaatcaattg acggtagaaa cagcaaaaga gattggtgca
    15661 gatgcgttga tcagaggcat acgaagtatc aaagactttg aatatgaacg agagattgct
    15721 cagatgaatc atcatctata tgatgaattg gaaactgtct tcttattagc aaaaccagaa
    15781 tatagtcacg tgagttccag tattttaaaa gaagtactgc actttggcgg agatgtgagc
    15841 agctacttac cgccagtgat caacgaagct ttagctggaa agcgtgaatc aaatgaaagc
    15901 taaacaatgg ttgaaaacag tactgattat catatttgga atgttgatca ttggatttgt
    15961 agtagttccc ttgccgtatt atgtagaatc accgggagcc acgatcgatc tgaaagaact
    16021 aattacggtc aatgataagg aagataaagg ttctgggtct ttctctctta cctctgttgg
    16081 tatcaggcaa gctacaggtt tcttagcttt aaaagctaaa atgacccctt ttgaagaatt
    16141 gatcagtgaa gaagaattga ctggtggggc gacaaatgaa gaatataatc aaatgcaaca
    16201 gtattatatg gattcttccc aaaatgcagc aatcgaacaa gcgttaaaac ttgcagacaa
    16261 gccttataaa atggaattca aaggcgtata tgtcatgagt gtagagaaaa catcgaattt
    16321 ctttggcaag ctttcagttg gagatacagt aacaaaagta gacgggaaaa gctttcaaag
    16381 tacagaagca tttatggatt atgtaaaaag ccaaaaagtt ggacaaacaa tcgaaatcag
    16441 ttatttgcat aatggtgaag aaaaaacagc ttctggtgat ctgatcgagt taccaacaga
    16501 taaaaaggca ggaataggta tcacgcttac tgaccatacc gagatcgaat cagatacaga
    16561 tgttcgtatt aattctggct ctatcggtgg gccatcagcg ggattgatgt tcactttaga
    16621 aatctatgaa caagtcactg gtaaaaactt gcgacatggt aagcaaattg caggaactgg
    16681 aacgatcaac agtgaaggag aagttggccg aatcggagga atcgataaaa aagtggcaag
    16741 tgcggataaa gctggcgtag agattttctt tgcaccagat gatacgattt cagacgatgt
    16801 taagaaagaa tatcctgaaa taaaaaccaa ctacgaagaa gcaaaagctg ccgctaaaaa
    16861 attgaattcc tcaatgaaaa tcgtaccagt aaaaacagta caagaagcgc ttgactatct
    16921 agagaaaatg aaatgaaact aaataattaa agaggggttg tgacacgagt tctgtcacaa
    16981 cccctctttt tcgtcaaaaa t
//
LOCUS       DACGLW010000066        16660 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.069, whole genome shotgun sequence.
ACCESSION   DACGLW010000066 DACGLW010000000
VERSION     DACGLW010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16660
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.069"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            complement(<1..194)
                     /locus_tag="IXT53_002098"
     CDS             complement(<1..194)
                     /locus_tag="IXT53_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAQ4905895.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPSPLINRLVNYLYFTAYT"
     gene            complement(196..393)
                     /locus_tag="IXT53_002099"
     CDS             complement(196..393)
                     /locus_tag="IXT53_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterocin"
                     /protein_id="HAQ4905896.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(787..2514)
                     /locus_tag="IXT53_002100"
     CDS             complement(787..2514)
                     /locus_tag="IXT53_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ4905897.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2507..3700)
                     /locus_tag="IXT53_002101"
     CDS             complement(2507..3700)
                     /locus_tag="IXT53_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HAQ4905898.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3887..4531)
                     /locus_tag="IXT53_002102"
     CDS             complement(3887..4531)
                     /locus_tag="IXT53_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ4905899.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4625..4759)
                     /locus_tag="IXT53_002103"
     CDS             complement(4625..4759)
                     /locus_tag="IXT53_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HAQ4905900.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4761..6893)
                     /gene="feoB"
                     /locus_tag="IXT53_002104"
     CDS             complement(4761..6893)
                     /gene="feoB"
                     /locus_tag="IXT53_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HAQ4905901.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6890..7363)
                     /locus_tag="IXT53_002105"
     CDS             complement(6890..7363)
                     /locus_tag="IXT53_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HAQ4905902.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7764..7988
                     /gene="nrdH"
                     /locus_tag="IXT53_002106"
     CDS             7764..7988
                     /gene="nrdH"
                     /locus_tag="IXT53_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HAQ4905903.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8147..10306
                     /gene="nrdE"
                     /locus_tag="IXT53_002107"
     CDS             8147..10306
                     /gene="nrdE"
                     /locus_tag="IXT53_002107"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HAQ4905904.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10356..11321
                     /gene="nrdF"
                     /locus_tag="IXT53_002108"
     CDS             10356..11321
                     /gene="nrdF"
                     /locus_tag="IXT53_002108"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HAQ4905905.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11410..12549)
                     /locus_tag="IXT53_002109"
     CDS             complement(11410..12549)
                     /locus_tag="IXT53_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HAQ4905906.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12858..13571)
                     /locus_tag="IXT53_002110"
     CDS             complement(12858..13571)
                     /locus_tag="IXT53_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HAQ4905907.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13825..14526)
                     /gene="nagB"
                     /locus_tag="IXT53_002111"
     CDS             complement(13825..14526)
                     /gene="nagB"
                     /locus_tag="IXT53_002111"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HAQ4905908.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14760..16292
                     /locus_tag="IXT53_002112"
     CDS             14760..16292
                     /locus_tag="IXT53_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ4905909.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16483..>16660)
                     /locus_tag="IXT53_002113"
     CDS             complement(16483..>16660)
                     /locus_tag="IXT53_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ4905910.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         5175 a         3429 c         2879 g         5177 t
ORIGIN      
        1 ttagtataag cagtaaaata tagataattc accaatctat ttattaaagg actgggatct
       61 aattcatatt tttgtttttc taattttaaa tatacctttt gtaaaacctc aagaatatca
      121 gaatatttag gatctttact tatagatata tcattatata attcatgaac aatttgctta
      181 gcattttttt tcattttagc acttccctgg aattgctcca cctaaaaaac cacctataga
      241 cattcctgca atacaagtag ttgccttggc ccaatcgacc gtacatttat ttttagtgca
      301 atacactcca tttccataat attttccact atgagtggta cccccataga taagttgtgt
      361 ctctttaata gacaaaattt ttaaatgttt cataatctaa tcacccctaa attaatgttt
      421 ttagactatt attctaacat tcattaaatt tcaaaaaaca attccttctt gaaacgtagc
      481 aaaataattc ctgaataaga atttaatcac ttttaagtat ataaactatg tatattcttt
      541 aatatttttt gagaatgcac ttcgtacata ataaacttta atcacattag tctttcatac
      601 ctacgatcga tctctttgac tcgcacccat aaattcataa tagtttgtcg gcgtctttga
      661 acgtttcgaa gatgagctac gcattctctt tttttataga caaaaaagat agagacgaac
      721 atggagcttc caaatttgga tatttgatcc aattttggaa gctccattta tttcgtgtct
      781 gtctttttat tttttccagc tttcgactaa ttcttggttc tctttgatcc agtcttttgc
      841 tgcttgctgt ggatcttttc cattgttgat ttccaacata actttttcca tatctttttc
      901 tgaccaatgg aaattgtcta atactttgta agcttctggc tggtcttctt tcaatccttt
      961 tcgaaccatc gtatggatct gctcttcgct tcccattccg ttttcaggat cttctaagta
     1021 tttcagatca tatttggcaa acatccaatg aggtgtccat cctgtaacta caattggttc
     1081 gtgttttttg atcgcttgac ctagtgcaac ggtcattgct cctgaagaag atgtctctac
     1141 tttccagtct ttcaagttgt catatttttg gatcgtgttt tcagcagccg taactacacc
     1201 agctcctggc tcgattccta tgattttctt acctgcctga tcggttaact catcgatcga
     1261 atttacatcc atgtaggctg gcactgctaa accaactttt gctcctgtca aattggcacc
     1321 taggtcttcg acttgatctt tatattgtgc atattgtgaa ccatgagttt taggtagcca
     1381 agctgaaacc atggcatctg actcgccatt tgaaacagat ttccacatga ttgaattatc
     1441 taatggcgtc atcgttacat catagcccat ttgtttcaat acttcaccga ctacatttgt
     1501 tgaagcaact tctgtatccc attcaacata agataagttg atacgttttg tttctttggc
     1561 agatgaaaaa ctccctatac caatcgcacc taagatcact actactacag caccaatgat
     1621 cagtccttga cgttttttac ttttctgtgt tgttgctcct gcagcttttt tattgtttgg
     1681 ctggttcaaa tgctgtgtaa aacggtcaat gatgattgct aagataacta atgcaacccc
     1741 gttgacaaat ccattcccga cttgtgctct ttgcaatgct gataatacgc cgcgtcctaa
     1801 gccgggtgca ccgatcatag aggcaataac gaccattgaa agtgacagca ttgttgtttg
     1861 attgatccct gccatgatcg tacttttagc taaaggcaat tctagtttga atagtttttg
     1921 acgacttgtg ctcccgaatg aatcagaagc ttctactaat tctttaggaa cttgtcgaat
     1981 ccctaaattc gtaaaacgaa cggttggtgg cagagcaaag atgactgagg caaatacccc
     2041 tggaaccatc ccgataccga aaaatgcaac tgctggaatc aaataaacga atcctggcat
     2101 tgtctgcata aagtcaagga tcggtttgat aatactttgg gctttactgc tttttgccat
     2161 caagatccct aatggtaccc caatgatgat cgagatcaca ctagataaaa gaacaagtgt
     2221 gacagtattc atcaaatcat tccatagacc ttggttataa ataaaccata gaccgattaa
     2281 tgtgaacaac gtaagtcctg gacgtttctt agaaataaag aaagctgcta ccgtcaataa
     2341 caagataaac aaaggagctg gaatcactaa cagtaaattt gtaatccccg acatcactgc
     2401 ctgtccaatc gtttgtaaaa atgaaaatag gccagaaaaa gttgtcgtaa accattctgt
     2461 gatattttct acccagtttg ccactggtaa ttgatagttc aatagattat tcatgttcac
     2521 tcacctcgct tgtttctgct aatgcttcaa tcacactacc tcggatgacg acacctttta
     2581 attttccatc ctctacaact gcaagtggag tcggtgagtc ataaataata ttgaaaatat
     2641 cacttaccac catgtctttc ccaatcaccg tcacattttc atctaggaag tcgatcaatg
     2701 gacgtttttg cttcctagct tctaacgctt gatctgcagt gatgatccct ttcaggtgtc
     2761 tctttctgtc aacagccatc agcatgctga cttcttcatt acgcatcctt gtcaatgcaa
     2821 cgtttggtcc atcagattcg atattcgtcg tcaatgcagg taccatgatg ttttgagccg
     2881 taagcacttt ggaacgatcg acttcttcca caaattcacg tacataatca tttgctggat
     2941 tggtcaatat ttcttcacct gttccgatct gcatgatttc gccgtctttc atcaaggcga
     3001 ttctgtcccc aatccgtaaa gcttcattca aatcatgggt aatgaaaata atcgtttttt
     3061 gaacattggc ttgcaggtcc agtaattcgt cttgcatttc tcgccggatc agcggatcaa
     3121 gagccgagaa cgcttcgtcc atcagcaaga tctcaggatc atttgctaaa gctctggcta
     3181 ggccaacccg ttgttgcatc ccaccagata actgactagg atattggtct ttaaatgaaa
     3241 gcaaactaga attttctaat gctttttctg ctttttcttg tcgttcttct ttaggtacac
     3301 cacggacttc taacccgtat tctgtatttt ccaagatcgt tcgatgtgga aacagaccga
     3361 agttttggaa aaccatattg attttatgac gacggacttc tcgcaattct tctttgctta
     3421 acttcgatac atcttctcca tcaatataga tactgcccga agttggctca atcagacgat
     3481 tcaatagacg gatcaatgtg gatttcccac tcccggaaag ccccatgatc acgaagattt
     3541 ctccttcttt tacgtcaaaa ttcacgtcat acacaccaac cgttgcaccg gttttcttta
     3601 aaatctccgt tttcggctgg tgatcattca tcatatctaa agcggcttgt gtctttttac
     3661 caaatatctt tgttaaatgt tttacttcta cttttaccaa acaaattctc cttttttatg
     3721 taaattcatt tttctttctg aaaaaagtga ccacactatg ttaacatcat gtagtcactt
     3781 tgtcaaaaat gaaagaaatt tgcttgtcca aactgaaaaa aacttggcta aatctttgta
     3841 aataaaaggt ttagccaagt taatattttt ttaatatttc acacaatcat tctgcaagct
     3901 gatgctccgg gtaaaaaaag tttgtcatac gctgtagtgt atattcattt ccaataaaat
     3961 gaacgatgtc ccctgccgta aatttagcat aggggccagg tgaaacaagc agttcttcat
     4021 tatgagaaat tgcgataact gtggcgccag tcgcctgcca gatattcaac tcgctaattg
     4081 aacggtccag atgcattgct ttttctgata aagataattc aaacggaagt aaaggattca
     4141 tcttatgtat tcgcttcgtt tgattgacca gctgatccaa taattgagaa aaatgattca
     4201 gctcatcttg ttgcttctcc acacttgcca tgatttctgc ttttgtatct tggatggatt
     4261 gtacatcttg atattgcaat aggaaatctt ttgctttttc cttcgaagca acaaatgccc
     4321 cacttccatg ccgtacttcc atgattccaa gatctaccag cacattgata gccttgcggg
     4381 tagtctcagg agaagcattg tacgtactgg caagtgttga gcgggcatgg atcttctcac
     4441 ccactttata tcggttttca acgatacgtt cggctaaatc aacagcaatc tgctggtagc
     4501 gcgggcggac gatttttttc tttccagaca ttttctttcc tctttctttt tttactttcc
     4561 ctcatcttac tatgctctag caaaataagc aaaaaagggg agaatagcct cttgcaacga
     4621 ctgatcatcg cttgatctgt tcctttttta ctgggcaagc tgtttgacag tcatcacaat
     4681 tcttaccgga ttttaaacga gtatagacaa ttgcgcctgc acttccaaag attaacacac
     4741 ttaataagaa tgtcgccatc ctattcctct ccttctaatg acaatatagt ataaaactgg
     4801 ttcttggatt ttggctgacg gaacaaaaca taaacgagtc ctatcatcag taaaatagca
     4861 agatacgtcc ctaacgctac ctgtccaccc tccaataagt gtcccaactg ataaatcaca
     4921 aaactaacta aataagctaa accgcattga tagcctatgg cgatccaagt ccatttcttg
     4981 gcattcattt cccgatggat agccccaatt gccgcaaaac aaggcgcaca aagtaaattg
     5041 aaggctaaca atgaataagc tgctgcggga gtatatgtgt gttgtagcac ttgccagatt
     5101 tctctgccat tttcggatac ttccgcatga ccaaacaaaa taccaaatgt gccaataata
     5161 ttttctttag cgaccaatcc tgtaatcgct gcgactgttc cttgccagtt tccccaacca
     5221 agcggctgaa aaacaactgc caggattcgc ccgaaaaagg ctaagatact ttggtcttca
     5281 gaaacaggtt gagcataaaa attataagaa gagctgaacc agatcacgat actagaaaca
     5341 aagataatcg ttcctgcttt tttaacaaaa gacttgctgc ggtcataggt ttgtcttagg
     5401 accgctgacc attgaggaaa atgatatgcg ggcaattcca tgataaaagg agctgtatct
     5461 ccagcaaaca aacgtgtttt tttcaagctg attccagaga aaataattgc agtcatacca
     5521 agaaaatagg cagaaggagc tacccaagat tgatgaggaa aaaaggcccc tgctactaag
     5581 gcaataatcg gcaactttgc agaacaaggc atgaaggttg tgaccatgat cgtcaaacga
     5641 cgatcttgct cgttttcaat cgtgcgactg gccatgacgc ctggcactcc acatcctgta
     5701 gcaatcagca taggaatgaa ggattttcct gaaagaccga atttcctgaa aagtcgatcc
     5761 atgacaaaag cgatcctcgc catatatccg cagtcttcca gaaagctcaa gcataaaaac
     5821 aatataatca attgcggtag aaatcctaac actgctccca cgcctgcgat gatgccgttc
     5881 aagatcagct cctgcatcca tcccgctact tgccattcag ctaacaatct gccaacatgt
     5941 tctggtaacc atgtaccaaa caatgtgtca ttgatccaat ctgtccccat tgttcctact
     6001 gtttgaatag ccagataata gatcagccac atcacaaatg cgaagatcgg caaggctaac
     6061 caacgattcg ttgcaatctg atcgattttg tcactcatcg ataattggaa agaatcattt
     6121 gacacaacac agagtgtaca tagttgggta ataaattcat aacgttcatt gaccagaatc
     6181 gtctcactat cgtcgtccaa tagtttctct gttaactgga tcagttgctc gatctctttt
     6241 tgctgagaaa ctgctatctc gtaatcctct cttaccttct gatctcgttc aaaatatttc
     6301 attgcatacc agcgtttctt gccgctttgt tctcctagta catcttcaat ttcatgtaaa
     6361 gctgcttcta aacgtggatc atatgtacag ggaacaggaa tagaaggccg cttttttgca
     6421 gactcctgaa tcttttcgat tagtttttcg attccttgct ttttcaatac gctgattggt
     6481 aaaactggta caccaagacc ataggaaagc ttttctagat tgattttttt tccttctttt
     6541 ttgatgacat ccatcatatt cagaccaatg atcataggaa tgtctaaatc caataactgc
     6601 gtagttaaat aaagatttcg ttctaaattg gtagcatcaa taatgttgat gatcagttca
     6661 ggctgttcct gaaccaaata atctcttgca atcacttctt ctggtgtata aggagacaaa
     6721 gaatagatgc cgggtaggtc ctgaatatga atgttcggat tttttttcaa cgatccttct
     6781 tttttttcta ctgttacgcc cggccaattc cctacatatt gattcgaacc agtcaagaga
     6841 ttgaacaagc tagttttccc gctgtttgga tttccgacta acgcgatatt cattcttttt
     6901 cctccctttg tatcaagatt agttcggcct ctgactttcg taacgtcagt tcataccctc
     6961 gcactgtcag ttcgattgga tctcccagag gggctagttt tcggataact atttttacat
     7021 ttcgcgtcaa tcccatatcc attaaccgtc gcttcactgc gccttgtccg tggattgcta
     7081 cgacgatccc agtctctcct gcttttaatt gatcaagaga agtccagctt tcagaggaag
     7141 cagtcttttt attgacaaag atttgaccta gtatttcccg acttactgcc actcgatgac
     7201 tttttaccag taaaatggca ttgttgcctg ataaattgac gacgacgatt ttacttccgg
     7261 caacgatccc gatattttgc aaatggcgtt ttgttttttc ttctgtttcg attcgttcga
     7321 cttgataaac gaaattctgc tttgcctgcg ctaaggtctg catattgatt cctctcttgt
     7381 gaaataaatc atgtattaaa atcattcatt atatgttcta attttcttac gtgttacgca
     7441 aataaatgag aatcattctc acatagaata ctctcggtaa ttgagaatgt caattatact
     7501 tgtgatatta tgagcgaatc tttagtattg atagataaaa atgagaaaaa aatatttctt
     7561 catagttttc cacaattttt tctctattaa atttgctatt ttcccacacg atacttgttt
     7621 tcagctcttt tttcaaaaac acaaaactta gtattccaca gtttccacaa cacaatatgt
     7681 agtgtttgtt gtttgacttt tttactaaga gttttatact atctattata gataataaga
     7741 atgtgttaga ggaggcactc accatgaacg taaaaatctt ttctaaaaat aattgtatgc
     7801 aatgtaagat ggtcaaacgg tttttaacag aaaatcaaat tgcttttgaa gaagtcaata
     7861 tcgatgaaca gcctgaagca gtagactggt tgaaagcaca aggattccaa agtgttcctg
     7921 tcattacttc tgatgcagaa acagttatcg gattccgtcc tgatcagttg cgtaaacttg
     7981 ccagctaaca tgactaaaag aatcaaaacg tgatacatgg atttgaaggg cttgagacag
     8041 caccagtttt tgtcgcaaac ttccaaatcc ttttttaaag cttaaaaaca atgcaatgat
     8101 ttgcaatcaa gcatcatcaa atacgaaatg aaggttgggc gtacgtatga gtcttaaaaa
     8161 tttacaagat gtcagttatt tcaagctgaa taatgaaatc aatcggccag tcaatggtca
     8221 aatccctttg aataaggata aagaagcttt agttgccttt tttgaggaaa atgtcaaacc
     8281 aaatacgatg acttttccat ctattatgga aaaaatcaat tacttgatcg atcaagaata
     8341 tatagaagca gaattcattc aattgtattc accatcattt attgaaaagc tatatcaatt
     8401 tttggacgag caacattttt ctttcaaatc ttttatggct gcttacaaat tttattctca
     8461 atatgcgttg aaaaccaacg acggttccgc ttatctggaa acttatgaag atcgagtagc
     8521 cttcaatgcg ctttactttg cgaacgggga tgaagaatta gcacttgctc ttgcagacga
     8581 aatgatccat caacgatatc agccagctac accttctttc ttgaatgctg gacgtaaacg
     8641 tcggggcgaa ttggtgtctt gtttcttgac tcaagtcact gacgatatga actctatcgg
     8701 gcgctcaatc aactctgctt tgcaattgtc ccgtattggt ggcggcgtag ggattacttt
     8761 atcaaatctt cgtgaagccg gcgctcctat caaaggatat gagggtgctg caagtggtgt
     8821 cgttccagtc atgaaattat tcgaagatag tttcagctat tctaatcaat taggtcagcg
     8881 tcaaggagct ggcgtggttt atttgaacgt tttccatcca gacattattg ctttcctttc
     8941 agcaaaaaaa gaaaatgcag atgaaaagat tcgcgtaaaa actctttctc ttggcgtgat
     9001 cgtacctgat aaattttatg aattgacacg taaaaacgaa gatatgtatt tatttagtcc
     9061 atatagtgtc gaaaaagaat atggtgtccc attctcttat gtaaatatta cagctgagta
     9121 tgatcatcta gttgcaaatc caaacattcg caaacaaaaa atcaaagcgc gtgatcttga
     9181 aaatgaaatc tcaaaattac aacaagaatc tggctatcct tacgtaatca atattgatac
     9241 agcgaaccgc gagaatccaa tcgatggcaa gatcatcatg agtaatttat gttcagagat
     9301 tttacaagtt caaacgcctt ctgtgattaa cggcaaacag gaatatgaag tattaggaac
     9361 agatatctca tgtaacttag gctcgacaaa tatcgtgaat ttgatgcaaa gccctaactt
     9421 tggtcaatct gtgcgtacga tgactcgtgc attgacattt attacagatg catctgatat
     9481 cgatgtcgta ccaccgatcc aaaacggaaa taagctgaat catacgattg gattaggtgc
     9541 catgggctta cacacttatc ttgcaaaaga acaaatggaa tatggctcag aggattcatt
     9601 agactttaca aatatttact tcatgctgtt gaattattgg acacttgttg aaagcaacaa
     9661 tatcgctcgt gaaagaaaac aagttttcca taattttgaa aaatcagctt atgctgacgg
     9721 cacttatttc gaaaaatacg taactggaga attccagcca aaatcagaaa aagtcaaagc
     9781 attgtttgaa ggcattttta tcccaactgc tgaagattgg aatgctttga aacaagcagt
     9841 aatgaaagat ggactgtatc atcaaaatcg cttggcagta gcaccaaatg gctcaatttc
     9901 atatatcaac gatacgagtg caagtattca tcctatcact cgcatgatcg aagaacgcca
     9961 agagaagaaa atcggtaaaa tttattaccc agcgccttat ctatccaatg agacgattcc
    10021 gtattacact tctgcttatg atatggacat gcgtaaagta atcgatgttt atgcaacggc
    10081 acaacaacac gtcgatcaag gcatgagttt gactttattc atgcgttctg aaatcccaga
    10141 aggcatttat gaatggaaga caacaacgaa gcaaacgaca cgtgatttga atattttaag
    10201 acattacgct ttccacaaag gaatcaaatc catttattac gtacgtacct ttactgatga
    10261 tgcagaagaa atcggaagca atcaatgtga aagctgcgtg atctaatatc atagatagat
    10321 tcgcttattc atattttaat taaaggagca ttctcatgtc agaaacatat tatgaagcaa
    10381 taaactggaa tgaaattgaa gatatcattg ataaatccac ttgggagaag ctgacagagc
    10441 aattttggct agatacacgt atcccacttt caaatgacct agatgattgg cgtacattgt
    10501 ctcaattgga aaaagataca gtaggacatg tatttggcgg tttgacactg ctagatactg
    10561 tccaatctga aagtggcatg gatcagttgc gtaaagatgt ccgcactccc catgaagaag
    10621 ccgttttgaa caatattcaa ttcatggaat ccgttcatgc caaaagttat tcttctattt
    10681 ttagtacact gaatacgaaa aaagaaatcg aagaaatttt tgactggaca aatacgaatc
    10741 cttatctgca aaaaaaagct gagcgtatca atgaaatcta caaacacggt acaccgctag
    10801 aaaagaaaat tgccagcgtc tttttagaaa cattcttgtt ctattcaggt ttttatactc
    10861 ctctttatta tttaggaaac aacaaattgg ctaacgtagc agagatcatc aaactaatca
    10921 tccgagatga atctgtacat ggaacctaca tcggttacaa attccaatta ggattcaacg
    10981 agttacctga agcagaacaa gaaacgttaa aagactggat gtacaatctt ctttatgagc
    11041 tttacgaaaa cgaagaacgt tatacagaag aattatacga tccaatcggc tggacagaag
    11101 aagtcaagac attcctacgc tataatgcca acaaagcatt gatgaaccta gggatggatc
    11161 cactctttcc agatactgcc aatgatgtca acccaatcgt aatgaacggg atttcaacag
    11221 gcacaagcaa ccatgacttc ttctcacaag tcggaaacgg ctatttgtta ggtcatgtag
    11281 aagcgatgaa agacgacgat tatttgattg gattggaata aggatttatt ttaattcaaa
    11341 aatttttcga aaaataaaaa gagtttagcg tacagatatt ttaaatgtac actgaactct
    11401 tttttatgct tattcacttg tttcttccag cacttttgga ctgtctaact tttccgaaac
    11461 atgttccatt cttctcaata aatagatttt ccggatattt aatattatcg tcttaatcac
    11521 tgcatagaca ggtatgcaaa tcagcatgcc taatattcct gcgacacttc ctgctccaat
    11581 caataccaag atgatcgtca aaggatggat attcattgat ttcccaaata acaacggctt
    11641 gatcaaattt ccatctaatt gttggatcac taaaatcgac aacgccatat acaaagcttg
    11701 aaatggagag ataaacaaac cgacaatgat tgctggtacc gctccaataa aagggccgac
    11761 atacgggata atattcgtga ttccgcaaat gaccgcaagc aacaagctat aaggctgatc
    11821 gaatattctc attgccaaaa agctcattac accaataatc aaagcatcta agatcgtact
    11881 gctgatatag gcagataatg tttcgtttaa ttcttttacc gtttggcgta actctttacg
    11941 aatcgctttc ggaaagaagt gagacaacgc atcaataaat ttgtgcccat ccttgaacat
    12001 aaatagcaaa ataaatggaa cggtaaataa caaaataaaa aattgcatca taaatgaaat
    12061 gatttttgag acacttgcag ataaactgac taatatgata ttgaacaaat tactaagaga
    12121 taaatttgct gatgctaatt gttcttccaa attgaaattt ttaaactctt cccgctgcgc
    12181 caattcattt aaccatttgc ttgtttcatc tgcatacatt ggcaagctct gggtcaattg
    12241 aattgtctgt tccattaact gaggcactac attcatgaca gttaaaacaa ctaaaatgat
    12301 tacgaccaaa aacgtcaata aaaaaccgaa cactctcggg atccgatgct tttcgagaaa
    12361 aacaacgatt ggatcgaaca tataatagat aaaaccagca acaagcaatg gtaaaaaaat
    12421 tgccgaaagc attttcaata ttggactaaa aatattcggc atttgtaaaa taaagtacac
    12481 gccgatgatc gtgagtacta gctccactgt ccagaaaaat aattttgatt gtttaaaacg
    12541 tgattgcata catctcctcc aaggtccttt gatacttcta ttctatcatt taacaaagag
    12601 ttacgcgctg agattttcat ttgcttaaaa aaaacaaaaa aaataaacat attttaatga
    12661 gtagacgagt acttaaacaa aaacacggtt tggagtagtg acaaaaatta tactgcagtc
    12721 cctttttcca aataaacggt gttcaaaagc ttgcatcttc gtaataagcc cagccaaacg
    12781 aaaaatataa ggaactattt ttatttggct gggcttatta cttgatgtag gacatctatt
    12841 ttacaacctc agatcattta ttgcgcaata ctgactaaat gcaactttgt gatatttttg
    12901 tgtacggaca actcctcgat cacatcggca taagtgagcc cttttggaag atcaatcgta
    12961 tagatatttt tgtacactcg gtagtcatca acgaatttta cgtcaaaatt cacatcttcg
    13021 attacgatat ttttctcttc aaaataatcg gtaagaaaat ctttggtttc ttttctatgg
    13081 ataaattgga tttctaactt tttagtcgta ggtacatgga tcacgcgttt cactactgtg
    13141 agtgcaatag caatcccgat aaaactgatg atcgcaatgg aatagtaacc catgccgatg
    13201 gcgatcccta atccagcaat tgcccataaa gaagcggcag tcgtaagtcc cgtcaccgat
    13261 tgtttcgtga cgatgatcgt tcccgcgcct aagaaaccga caccactcac cacttgagca
    13321 atcagccttg cttcatctga acgaatcgtt ccagcaagct gtggatactc taaagcatct
    13381 cttaatgcac cactagcgat ctcaacttgg atcaatgcaa tgatacatgc tcccatacaa
    13441 actaaaatat gcgtacgcat acctgctgga cgatttttat attgtcgttc aaaaccgatt
    13501 gctccgccaa atagcatagc gatccctaaa cgtaaaataa tctctggaat tgtcaatacc
    13561 atatcattca tctaaacacc ctccagtcca atatatcatg tctgaaagga gcagacaaaa
    13621 aaagtgcaat ttttacctct tacgaaaaaa ggactgtgac aaaatttttg tcatagtcct
    13681 ttttatgaat aaacggtgtt caatcagctg accctcggta ttactcaaca aacggcaaaa
    13741 tatggggagc tatttttcct ttgtcttctc taatactcag gtcaaaacgc tgatttcaca
    13801 acctctttag cctactattg aaaattacaa cttacttgct gcttcttcat cgatgatcac
    13861 aacaacattg tcgtgatttt gcaatacact tgctggcaaa tgattcgtta caggtccttc
    13921 aatcatccct ttgattgcat cagctttgct ttcgccaaat gccatcaaga cgatttcttt
    13981 acttttcata attgaaccga ttcccattga aacagctttt gttggtacat cttctgcttt
    14041 atcgaagaat cgtttgtttg cattgattgt tgattctgtt aagtcaacta ctgaagtcgt
    14101 tccgtcaaat gaagcaccag gttcattgaa accgatatgt ccattttgcc cgattcctaa
    14161 gatttggata tcgattggat gtgcatcgat gatttgatca tactttttgc atgctgcttc
    14221 cagatcttct gcttttccat ctggtacaaa cgtttctttg aatggttttt tatcaaataa
    14281 ttggacatcc atgaaatgac ggtagctttg ttcatctgaa ccgcctagtc caacgtactc
    14341 atctaagttt acagaagtca tttcactgaa atccaaatcg cttgaagtca tttctttata
    14401 taaagtaact ggtgtgcttc ctgttgccaa tcctaatact tttgcacctt tgttcatacc
    14461 ttctttgatg atttcaaatg ctttttttcc gccttcttca gcgtttttta cacgaatgat
    14521 ttccatagtg attgctcctt tatattttgg tatagcccaa ttatagcatt ctttttttct
    14581 ttttacaata attagtatag accaaattgc atttttttca ttttcttgac aataaatagg
    14641 aatccgtgtt aggtttcata agatggttca atcaaacaat ccatgttgca tgaaaaccaa
    14701 gcaagtgatt cactgtggac gctttatgtc tgcagttttt ttatgaaaaa ggagttatta
    14761 tgagtatact gacattaaac aacattaccc agcaatttgg cgacaaaatc ctctatgaag
    14821 gtgtcgatct gcaattaaat gctggcgaac atcttgggct gattggacaa aacggagccg
    14881 gtaaaagtac attgattaaa atcatcactg gtgagatctt accagatgac ggacagatcc
    14941 attggcaaaa aaatattcac aaaggttacc tcgatcaata tgtggaagtc gatgaaacat
    15001 taacaatcga agaatttcta aaaactgcgt acgcaaaaga atacgaaaag gaagaaaaaa
    15061 ttgcttttct ttaccaaaca tatagcgaga ccttcgatga ttccttgctg gaaaaagctg
    15121 ggaaactcca aacggaatta gaccaaggag tgttttatca aatcgacacc ttagttgctg
    15181 agatgagcag cgggttgggg atcgatgtct taggtttaga ttcactgttg aaaaatctaa
    15241 gtggtggaca acgttcaaag gtgattttag caaaactact tttagaaaac ccagatgttc
    15301 tgatcttaga tgaaccaacc aatcacttag atgatcagca tatccaatgg ctgacgcagt
    15361 ttcttcagga ttttgacggt gcgtatatcg tgatttccca tgaccaagaa tttcttgatc
    15421 agatcaccac ccatatcgca gacatcgagt ttggaaagat tactaagtat actggccatc
    15481 taaaacaagc attgaaacaa aaggaacaaa atcgcgaaag ctatttgcgc caatatcatg
    15541 ctcaacaaaa acaaatcgaa aagaccgaag cttatattcg aaaatacaaa gccggttccc
    15601 gatcaacgat ggcaaaaagc cgacaaaaac aattagataa aatcgaacgg ctgacgcctc
    15661 cagcttcagc aagtaaagct gtctttgatt ttccattctc gcctattgtc acgacattag
    15721 cagtggaaac aactgattta gtcatcggat atgatcgacc gctacttgca cccatcaatt
    15781 tgaccatccg ctttggtgaa aatattgcca ttcgaggctt caacgggatt ggaaaatcta
    15841 ctttattaaa aacactgatc ggtgagattg aaaaaataag tggcgatttc cacttcccag
    15901 ataacacaaa aatcaattac ttttcacaag atctgtactg ggaaaatcca ttagaaacac
    15961 cactacaata tttaagcagt caatttccaa aagcgactat caaagaatta cgtcgccaac
    16021 tagctaaagc agggttagtt aatcaattag caagtgaacc tctcactaca ttaagtggtg
    16081 gggaacaaac taaggtgaaa cttgcacagt tgacaatgaa ccaaggtaat ttactgattc
    16141 tcgatgaacc gacgaaccat atcgatcaag aaacgaaaga aagcttacaa gacgggatac
    16201 agaaatttcc aggaacagta atcatcgtct ctcatgaaca agaattttat caagatcttg
    16261 tgcaacgagt cattgagatt gaaccaaaat aaacgtttga ttgaatagtt gaaaaaaagg
    16321 agctgtgaca aaagttttgt cacaactcct tcttctgaat aaatggtggc caattagctg
    16381 accttcggta ttagctcaaa aaacggcaaa atatgaggag ctatttttat ttggctattc
    16441 ttattacttg atgcagaaca gcttttcaca acctcttttc gtctattttt tattttcgag
    16501 atttctcttt cctttaaaaa caactgtttg aaagaagaac gcacatcctt gaaaaacaag
    16561 cccgctaacc gttacaccta accagccata ctgctgccac attgtcgtcc ctagcaatga
    16621 accagccgca cctccaataa agtacataaa cataaataca
//
LOCUS       DACGLW010000067        16641 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.070, whole genome shotgun sequence.
ACCESSION   DACGLW010000067 DACGLW010000000
VERSION     DACGLW010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16641
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.070"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            complement(133..840)
                     /locus_tag="IXT53_002114"
     CDS             complement(133..840)
                     /locus_tag="IXT53_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HAQ4905911.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(919..1257)
                     /locus_tag="IXT53_002115"
     CDS             complement(919..1257)
                     /locus_tag="IXT53_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HAQ4905912.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            complement(1368..2330)
                     /locus_tag="IXT53_002116"
     CDS             complement(1368..2330)
                     /locus_tag="IXT53_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HAQ4905913.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            complement(2345..3676)
                     /locus_tag="IXT53_002117"
     CDS             complement(2345..3676)
                     /locus_tag="IXT53_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ4905914.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            complement(3684..4364)
                     /locus_tag="IXT53_002118"
     CDS             complement(3684..4364)
                     /locus_tag="IXT53_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HAQ4905915.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            complement(4583..5062)
                     /locus_tag="IXT53_002119"
     CDS             complement(4583..5062)
                     /locus_tag="IXT53_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HAQ4905916.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            complement(5189..6868)
                     /locus_tag="IXT53_002120"
     CDS             complement(5189..6868)
                     /locus_tag="IXT53_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAQ4905917.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            7416..8918
                     /locus_tag="IXT53_002121"
     CDS             7416..8918
                     /locus_tag="IXT53_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ4905918.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            8931..9593
                     /locus_tag="IXT53_002122"
     CDS             8931..9593
                     /locus_tag="IXT53_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4905919.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(9694..9897)
                     /locus_tag="IXT53_002123"
     CDS             complement(9694..9897)
                     /locus_tag="IXT53_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HAQ4905920.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(10008..11192)
                     /gene="iadA"
                     /locus_tag="IXT53_002124"
     CDS             complement(10008..11192)
                     /gene="iadA"
                     /locus_tag="IXT53_002124"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HAQ4905921.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            complement(11349..13088)
                     /gene="yfcC"
                     /locus_tag="IXT53_002125"
     CDS             complement(11349..13088)
                     /gene="yfcC"
                     /locus_tag="IXT53_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAQ4905922.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            complement(13861..14475)
                     /locus_tag="IXT53_002126"
     CDS             complement(13861..14475)
                     /locus_tag="IXT53_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ4905923.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(15081..15458)
                     /locus_tag="IXT53_002127"
     CDS             complement(15081..15458)
                     /locus_tag="IXT53_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905924.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(15758..16639)
                     /gene="galU"
                     /locus_tag="IXT53_002128"
     CDS             complement(15758..16639)
                     /gene="galU"
                     /locus_tag="IXT53_002128"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HAQ4905925.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT         4828 a         3581 c         2842 g         5390 t
ORIGIN      
        1 ggttatgaga acaaaacaaa gaggatgtga caaaattctt gtcacatcct cttattccaa
       61 ataaatggta ttcaatcagc taactctcgg tattagctca gatcaaagcg ctgatttcac
      121 aacttcttta ttttaatctc ttcggttgcc gccgaacaat aagatcaagc gcaacagctg
      181 caggaatgtc gaaagcgctg ctgcaacata agtcagtgct gctgcaaata atacgtgtct
      241 tgccatcggt acttcttctt ccgtcagtag tccgccttca ctcaaaattt ggattgctcg
      301 ccgagacgcg ttaaattcga ctggtaatgt caccaattgg aaaagtaatg ctaaagaaaa
      361 agcgaaaatc ccgatattga ttaacgtaga gttccagcta agcaatacac ctaccataat
      421 caaggggaac gaaatcatcg aaccaaagtt agctgctgga acaagtgctg cacgaatctt
      481 caatgggaca taacccgaag catcttgtac cgcatgtccg cattcatgag ctgctacacc
      541 tatcgcagca actgaagtag actgtgcagt tgcttgagac aaactcaaca tcttattacc
      601 tgaattgtag ttatctgtga gatctccggc aatttgttga acaccgacat tatttatctg
      661 ttgactttgt aatatatatt gtgctgcttg tgtaccagtc acattgtttt tgcttctcac
      721 ttgatcgtat tttcggaaag tactattgac gtacgctgac gcaattccgg aaataactaa
      781 tccaagaatg accaaaagaa atgtcggatc taaaaaacca tacattccat aaccatacat
      841 tttcgtttca ctccttttcg tttactttat tttatcatta gtagtaggaa aagaatgtga
      901 aaagaccttg aatattcttt aattcttttt cacaaattcc gatttcagtt tcatcggtcc
      961 aaatccttct accttgcaat cgatattatg atcgccttct actaaacgga tgttcttgac
     1021 ttttgttcct tgtttaatgg cacctgacgc accttttact ttcaagtctt taatcactgt
     1081 cacgctatcg ccctctaaca gacggtttcc atttgcatct aataccacta atccgtcttc
     1141 tatgacagtt tcttgttctt ctttgttcca ttcgttcccg cattctgggc aaatcaacaa
     1201 gcctcgatct tcatatgtgt actctgagcc acattctggg caatttggta atgtcatctt
     1261 ctttctcctt taaaatcttt agtgagcact attgtaacgg aaaaagaaga tcttgaaaag
     1321 atacatttgt gatttttact acaaaaggca cgttttcgaa cgaagtatca ttctgttttt
     1381 ctgctcttat acgattggca tatatcttgg atctctttat agatttcctc tacttctgct
     1441 aaatcttttg cattcgcacc acttgctaat ggatggcctc ctccatgatg gcgtttagcc
     1501 aattcattga tgatcggccc cttagaacgt aaacgtacgc ggtaataccc tgaaggctgc
     1561 tgaacaaaaa tcccccaagc cagcacttcg tcaatcactc caggaagtga aacgatcgaa
     1621 gctgtctctg aatcatcgat tccatattta tcaagaattt cttgcggaag gattattctt
     1681 cctgcaccat tttcatccac ctggatattt tgataaagat aaccggaaag tttagcgact
     1741 ttcatcggca tttgttcaat ctctctgttc aaagcacttg catcaaatgg ataatttctc
     1801 aatatcgaag caatctctaa tgtacgggac gtcgtggagg gatacaaaaa gcgccctgta
     1861 tctccgacga ttcctgcata aagtaaccgt gcagccttaa cgttcatctt taaagattca
     1921 ggaaacatca aagcaaattc tgcaatcatc tcactacagc tacttgcttt tgtatttacc
     1981 cacacaagat ctccataagg gtcatcattg ggatgatggt cgattttgat cagtttttct
     2041 gccaactgat atctttgatc actgattctg ggtgaattag ctgtatcagt cacgatgact
     2101 agcgctcctt ggtagaagac atcttcaact tcttgcattt ctgctaaaaa gcgaagccct
     2161 tcaatcgttt cacctacttg acgtatttct ttttctggga aactttcccg taatagttcg
     2221 gctaatccta cttgtgatcc taatgcatca ggatcagggc gcaaatgcct gtggataatg
     2281 attttatcgt atttttttat tgtatctact atttcttgct gtactgtcat ttcactcctg
     2341 ctttctaaga acgttccatt acttgacaaa caacgattgc tttggaaacg atggtgtttt
     2401 caataaacac ttcgatatcc agtttagcgg aacgtctgcc gatttccaat acacgtggac
     2461 gaatgtctaa ttcgctttcc aattggatca gccgcaaata atgtaggttc acttgttcaa
     2521 tcaagatatt tcgtttttgg ttcatcacca ttgttttttg tgcgacatca gaaatgattt
     2581 cgctaagtac gccaaaagaa atggttccaa cgccattgac catttgtggt gctactacga
     2641 atttgaactt agggttttcc aaacgatttc cttcacggtc agtatcaatc gtatggatat
     2701 ttccagatat ctgatccgaa atcgtatctg caatctgcgg ttgtcgctgg acaagctgca
     2761 tcgctttcat tacatcttgc cgggtaatca agccttccaa tgtcaagtca tcggaaacga
     2821 ccggcatgac ttctagccca tcccagatca tttgatggct aacagaggca atactcattg
     2881 ttttctttac actgattggt tctttcgtca tcacccgatc gatgatctgg tttggcgatt
     2941 tccctaaaac atcttttgcc gtaacaatcc ccatcaaacg atgatggcgg ttgacgactg
     3001 gaaaacgcga atgctgcgtt tcttctgaca aacgctgata atcagaaatc gtatctgtct
     3061 gctgcaaata atgagtcttt tcagctggca tatagatatc actgacaagc atgatatctt
     3121 ttttgatcaa ttggtcgctt aacgctcggt tgatcatggt tgcgactgta aatgtatcat
     3181 aagaggtgcg taagatcggc atttcaagat catcagctag ctttgcaatc tcagcagtcg
     3241 tattgaatcc tcctgtgatc aatactgcgg cgccatcttc caatgctaat ttttggactt
     3301 ccgcccggtt gccgacgatc attagtgatc ctggcgtaat atagcgttcc atggcatgtt
     3361 tagtcatcgc gccgatgacg aatttattca agaccttgtc taatcctgcc gccccgccta
     3421 aaacgtcccc ttcaatgatc ctgacgacct cgccaaatgt caaacgttca atatgttttt
     3481 ttaatttgcg ttcgatacgg atggtcccta ctcgttgaat tgttgaaact agtccgacat
     3541 tttctgcatc tttgattgca cggtacgctg ttccttcact gacattcaat tcttttgcga
     3601 tgcttcttac agaaattcga ttgccaacag gtaaactttc aatatattct aaaatcaaat
     3661 catgttttgt cgccattttc tgcctacact tccagcgttt ccgagacgct cattattttt
     3721 ccttcactat cttccagtaa ctctacaaaa gattcgggat cttgttcaat gacagggaaa
     3781 gtattgtaat ggattggaac tactttcttg gcacgaattg tttgcgcagc ctttgctgca
     3841 tcttctggcc ccatcgtata gttatcgcca atcggcaaga aagcgacatc aatatcgaat
     3901 tcttctgcaa gcaatcctag atcactgaaa taagcagtat cccctgcatg ataaatcgtt
     3961 tttccttctg cttgaataat aattccgcta ggctcgccca tatactgaac gataccgtct
     4021 acttcatagc ttgaactatg ctgtgcatgt acgaatttta ccacgccgaa tggaaacgca
     4081 tgttttccac cgatattcat accgaatgaa tctactcctc tgctgttcgc ataatcagca
     4141 atctcaacaa tactgataat tgtggcttga ttttcttttg caataggaat catatctcct
     4201 atgtggtcac tatgaccatg agtaatcagc aaccaatctg tctttacttc ttctgcagtt
     4261 aagtctgtca atggatttcc tgtaataaac gggtcaatca tcacgcgttg gccattttcc
     4321 atttcaattg ttaaacagga atgtccatga tacgtaattt tcatatttta cacctcgcgt
     4381 tttctctgta tcttttttat taaaaacgat ttcatcactt gcacatcaat gaaaaaatat
     4441 agctgctaaa tatattttca ttggattgat agtacagtat gtacaaagca attgtaacag
     4501 ataatacagg atgcaccaag taccaagcag atataatctc aagtttgagg tctctgctct
     4561 gcttagcttc tcttctcgtc ccttaactaa ttaaatcagc gtaaatagga tggacgatct
     4621 tttctaaatc ggctactttt atccgaaggc ttctcccgat ttcaccgctt gacacaatta
     4681 cttcttcttt ttcttttaaa gaactgtctg ctaaaaaaca ataatcagga tgtgcgagtt
     4741 ttattccaat aggggtgttt gctccgtggg gatatcctgt atgcattaat acaaaatcca
     4801 ttccaggaaa accaacttta cgatctttcg tcacttgtct cgtttttttg taatctaagt
     4861 gatcgttcaa aggaatcaat gcgatgatca ccccagtttt gtttcctttc actacgagag
     4921 ttttacatat caaagctgga tcaatccctc ttttttgtag ttcttctgtg aaagattctt
     4981 ctaccacatg actaaaatca agtggttgat agctgatctt ttgttcttct aaataacgtt
     5041 cgacttttgt ttgactcatc attgattcac tccctttatt ctatcgttta ttataaagcg
     5101 agggatgctg tattcaaaaa ttatttgttt ttcgcaaaaa aagagcaaga cgagcactct
     5161 atagagaggt tcatcttact ctttttgttt attttttatt tcgacgttga gtagcttcca
     5221 taatgactgg caagatcact gggcggcgtt ttgtttgttc aaataagtaa cggcctaatt
     5281 gatctcggat gtcttgcttt aatttgctcc actcaaaatc attgctttct aaatgtttct
     5341 caacgattgt ttcaatcata ttgctgcttt ctttcatcag atcacggcta gcttttacgt
     5401 aaacgaatcc ccgtgaagta atttgaggtt ttgaaatgat tttcttttct cgccgactga
     5461 tagtgacaac cgctacgaaa atcccgtctt ctgacaagat ttttcgatca cgtaaaacaa
     5521 tgtttccgat atcacctatg cctaatccat caatcattac attttctgca cttgtagttc
     5581 ctgcaacact catacgcttg ttcttatatt ccagcacgtc tccgcgtcca gtaatataga
     5641 tatttttgta tggcatccct actgcatgag ccaaatctgc atgagcagct aattcacgat
     5701 actctccttg aactggcaca aaatatgttg gtttcatcaa attgatcatt aattgcaagt
     5761 cattaggatt ggcatggcca gaaacgcgca tattctcaga gatcaacttc actgttccac
     5821 cagctcgata aatgatatct tccgttttcg ccacgatggt ttccatcgcg atgcttggcg
     5881 tagtagtgat ataaaccaaa tctccttctt caatcttgac attacggtgt gtaccgtttg
     5941 ccattttttg cagagacttg atgggttcac ccattcggcc agtttctaaa atgatcagtt
     6001 cttctggatc atagtttttc atgtctttca acgtaacaag cagatcttca cttggtaatt
     6061 gaagtttttc taaacgcatc gctgtgcgaa taattcgttc gaaatcttga ccagtcaaga
     6121 caacttttcg ttgtgctttt tctgctgcat ccaatacttg ttgcactcgt tgcaaattac
     6181 ttgctacaca agcgacgatg atacgccctt cccaatattt gatcgtatca aatacttcat
     6241 ctgctatttg ggcttcagaa gctacaggct gaggattttc tgcatttgcc gattcactta
     6301 gtaaggctaa gactccttct ttgccgattt ccgccaatcg tgcataatct gtttgataca
     6361 ttgggatagc cgtttggtca aatttaaaat ctcccgtata aacgatacta ccttcttttg
     6421 ttttcagatt gatccccact gaatctggta tcgtatgagt ggtacggaaa aagctcacta
     6481 ctgtttcacc aaagtctatt tctgtatgct catctaccac atggaaatcc ttaaatcctt
     6541 tggctgcttc attacgactg acattcaatt ttgctaactc gatcgtcaac tctgtcccga
     6601 atactggtac agaaattttt gacaacaagt aaggaagtgc cccgatcgca tctgcatgtc
     6661 catgtgttaa aaagacacca gctacacgat caatgttttc ttctaaatac gtaaaatcag
     6721 ggattacgac gtcgatcccc aacaactcat tttcaggata ttttagccca caatctaaaa
     6781 taaaaatatc ctcgcccact tctgcgacat acatgttttt tccattttca cgaacgccgc
     6841 ctaatgggat aattttaatg gtactcacaa acttcacctc ttcttatgac atccaagttt
     6901 aaagatgtcg attttcttct ttatctaaat atatgagcca ttccaatgaa gcatatgcaa
     6961 aatcactatt tctcaactta tggagaatca gcaggaaaat taccagcaca tccgcgttct
     7021 gacagttgga tgggcatttt attttggata tacttcatta acaaaatgga tcttttgcga
     7081 aacgctatag ccagaatact tatataagta ttcagctgtt ccccattatt atacaacaaa
     7141 taaactaaat cgctagtatc agcaattgtt agaacaacta tgcacgtaaa aatccctctg
     7201 tagcatctag gatttctttt gctattcctc tttcatttct tcttcgtcgg tttgaaacat
     7261 acttttttca ttttaccaca ttttttgcaa taactatagt gactttttat atatacagaa
     7321 cctcatgttg atggtgtgca aacagcataa gatttaagct agctttaaga taggttgtct
     7381 attatcaaat gcatctacta ggagggatgc aaaatatgaa ttttatgaaa cgtgcatgga
     7441 aaagtacaaa agtaaaatgg ggacgttcat tgttattgtt tgccgtgttc acagccattc
     7501 ttatttttgt gctagctggc ctcacgattc gaagcgctgc agaagaagca gcgaatcaag
     7561 cacaaaaaga agttggagca acagtgactt taagtgccaa tcgagaagct tcatttaaaa
     7621 aacaggaaca aacttcttca agcggttcta ccactgaaga acgtccagat ccaggcagtt
     7681 tttctcttac accagtttcc ttaacggacg cggaaaaaat agctgaatta gataatgtaa
     7741 agagttattc ctttgaaagt tccgcctcag ctatggcaaa agatggaata actgccatca
     7801 ctagttctga ttcaagtgaa gacagttcag aaactactga tgcaggcggt gaacctgctg
     7861 gaggaatggg cgggaatccg caaatgactc aagcagactt tcaaatcagt ggtgtgtcag
     7921 caactgcaca aaatagtaat ttttctgatg gaacagcaaa aatcacggat ggtgaaggaa
     7981 tagatgacag cgacaaaggc acaaacaatg tagtcattga ttctactcta gcagaaacaa
     8041 atgacctggc agtcggagat actttcacaa tcacgagtac tgaagatgaa gataccactt
     8101 atgaaatgac gatcaaagga atttatgaat ccagcgaaac aagcggcagt atgggtatga
     8161 aattcaactt catgaatccg tcgaatacac tttacacttc ttacactttt gcaaatgaac
     8221 taaatgggac atctgacgat aacacgatag attctgctat ttacactcta tctgatccag
     8281 ataaaatgga tgagtttctc aaagaagctg agaagctgat cgacacagat acatttagct
     8341 tacaatcaaa tgattccatg tatcagtcaa tgttggaacc tttaaacaac gtggcaagtt
     8401 tttctaaaaa tgttgtttta ctagtggctg tagccggtat tatcatactt actctgatca
     8461 ttatgattac cattcgggag cgtcggcatg agttaggcgt cttgctatct ttaggagaat
     8521 ccagaagtaa aatcattctc caattgttta ccgaagtggc tatttgtatg attcttgcat
     8581 taggagttgc ttcgcttagc ggaaacgtcg tcgcgaatgc cgtgggacag cagctacttg
     8641 accaacagac agaaaccacg acacaaaacc agcaagctcc tggcggtgga caaatgccag
     8701 gtaacagaca agaacaaggc ggtcaacgcg atggtcaaca aggaaccacg aataatccat
     8761 ttgctgtttc tgatcaagtc agcgaattgg aaatcaatgt caaacctgca caattgggac
     8821 tattagccgg agtagcattt ggaatcagtc tgttttctgt ctttttagct tctataggga
     8881 tattaagatt aaacccaaga aaaatcctat taaactaaag gaggaatttg atgttacgga
     8941 cagaacaatt gggattttgg tatcaagatc cgcaagatgc gctttataaa gatgtagaac
     9001 tgaatttcga agctggaaaa atgtacgcta ttttaggagc aagtggttct ggaaaaacga
     9061 cttttctttc gcttatttct ggactagatg caccaaaaga aggaaaggta tactatcaag
     9121 atcgttcact gaacaaaatc gggttaagaa gttatcgtaa aaatgatgtc tcgattattt
     9181 tccaagctta caacttgctt ccttatttat cggctctaga caacgtcata gcagcaatgg
     9241 aaatcactaa gaccaagcaa gagaattatc gacaagttgc tttagatcga ctggctcgtg
     9301 taggaattga tgaaatatta gcaaaaaaac aggtgaccaa actttcggga ggtcagcagc
     9361 agcgagtcgc tatcgttcgt gctatctgct gtgaacatca actaatcgta gcggatgagc
     9421 ctactggaaa tctagatgaa acaacttcac aagatatcgt caaacttttc caagaaatcg
     9481 cccatgaaca aaatcgctgt attatcatcg tgactcatga gattgaagta gcaaaaagct
     9541 gtgatgaagt ctttgaacta aaaaataaaa aatttcaaaa aatcgaagtt taaaaaacag
     9601 gataatgatc aaataaaaaa tccgaaccat atctgtctcg cagatatggt tcggattttt
     9661 tatttgcttg attcaattag taataagtgg tttttatttt ttttcgtctt ttcgagaaaa
     9721 cttgtctttg acttcgtcgc ctaaatttcg agctttttct ttgatgtcag cacctagtga
     9781 ttgtgctttc ccttctgttt ctttgctctt gtcgtctgtt gctttgccgt atgcttcttt
     9841 tccttcaccg actactttat ctttcatatc tttagaacgt ccttttaaat ctgccattgt
     9901 gcttcctcct cttagtttgt ttattgatct accttcaggg taacatcaac aacagataca
     9961 gacaaacaga acgctcgttt tctaaaaaag atagttagag ctgttgatta aaaacttttt
    10021 tcgaaagtcc cccatacttt gacttctttt tcttggacca ttatttctcc tttagcgata
    10081 actgtatcaa tatcaagcgt ttctttatcc aagatacaca gatctgcatc agcaccaatt
    10141 tcgatttttc cttttttatc caatttcaat atacgtgctg gattactggt tatagctcgt
    10201 aaggcgattt ccaaagggat actttctttt tgcacagctt ctttgatccc tactagcaat
    10261 gccttggcac ttcctactcc taaccctagg aaatgattgt tttcatcaaa ataaggcaag
    10321 cttccttgtc catctgaggt catcgtgaaa ttatctaaag aaacatcttc ctcgatcaat
    10381 cgtttcagtg ctttgctgaa gcgaacttca ccatctgtct tctcccaaaa atcaggatct
    10441 tcgctagcag tgaagtcaat cgtgccacca cgtttggcaa atgccacgca agcttcaaat
    10501 aattctggtg tgcgatttgc atgagtaggc aaaaattgtg tgatcggaat ttctgtttct
    10561 tctacgattc gtgtcaatag ctctagcttt ctttttcctc cgcctaagtg gacattgatg
    10621 atcccagctt ttccggataa catgcctcct acacgagcat ctgctactgc acgagcgaac
    10681 tcttcaaaac ttggctgaga agaacggtga tcggatactg cgatttctcc gattccgata
    10741 attttatcaa ttgtcaaaaa gtctttgatg atagaatctg ttactgtttt taccggcaat
    10801 cggtacgaac cttcgtatac ataagtggag attccttctg cttccagacc tttcgctttg
    10861 ctgatcaacg cagtcatatc tctaccttct ccgtctgttc ccaaacaacc gacaactgtc
    10921 gttacaccag ctgtagtcaa ttggctcaat gtgacttctg gcgttcgatt ttgataaccg
    10981 ccttctcctc ctccacctaa aatatggaaa tgacagtcaa tgaaacccgg ggttagtact
    11041 tttccctcac ctgaaagttc ttctacttcc actccttcgt agccgccttc tagctgttct
    11101 tctacagcca aaatcttccc accggcaatc agtacatcca tttttcctaa gtaatctggt
    11161 gcaaacactt caatttgtcg aatgattttc atacattttt cctctttctt taagtataac
    11221 tgatgatagg atcttaaaaa ataatccaaa cgataggatc tttactcctt tgattttacc
    11281 gtaaaatgac ggttcttgga gtccttatct actgtttgga ttactttttt atttacaggt
    11341 ctccagtact actgcagacc tattgcaatg gcaatcatga tgaaaatagt tcctaaaaca
    11401 aacaggaatc cttgcatctt gatttggaat cttgcccatt ttgcccattc gatccgtgca
    11461 acacctagca caccaatcaa gctggctgat acgggtgtaa aagcgtccat aaaacctgaa
    11521 ccaagctgat aagcaagaac tgctacttgt ctggatacac ctactaaatc tgccagtgga
    11581 gccatgattg gcatagtcaa agctgcttgt cctgaatttg atgtaacaac caagttgaac
    11641 aaactttgga agatgtacat cgcccaagca ccaatgacag ctggaacacc ggtcaacgcc
    11701 gtcccgatcc catgcaaaat cgtattcaaa gctgaaggaa cagatgcatc agatcctccc
    11761 aagacaagaa gaatcccttt ggccatacct actacgattg ctgtaccagc taaatcagct
    11821 gcaccagatt ggaaagaaga agcgatgtca ttgactttca ttccatctaa tttgaaaaga
    11881 acagcaatga ttccagcaac taatcccatc acaaagaatt gagaagcgat ttctggaatg
    11941 taatagcctt tttgagtcac gccccaaacg atccagacaa ggacaactaa catctctaat
    12001 aagatcaatt tatgcccaag catgaatttt ttattttcat cagatgtctt tttgatatga
    12061 gaacggaaat aatcatctga tttgtaagtc aacgaacttt ctggtttttt acggattttt
    12121 tctgcatata tcatcatata accagcggct aatgcagtaa caactaccca cataatcaat
    12181 cggaatgtcg caccagacaa cacggggatt ccagcaatcc cttgagccac cgctacgcta
    12241 aacgggctca tccaagaggt cgcatttccg acttgagatg cgacataagt cacggttact
    12301 gctacgatcg agtcatatcc taacgcaatg acaaatggaa ccatcaccat tgagaatgga
    12361 attacctctt ctgccatccc gaatgtagcg ccaccgaatg aaaaagcaaa gaataatagc
    12421 ggtaaagcta aacgctccag tcccttagtt ttactgataa acgcatagat acctgcgtcg
    12481 atcgctccgg tacgcataat aataccaaaa gcacctccaa caacaaggat caatgcagcg
    12541 atccctacag cagatccata cttatctcca gatactagtc cttcaaaaac aaaatttaag
    12601 aaaccaaagc cgccaaaatc atctgttccc catattttgg ctgttttatg caatttttta
    12661 gaagtatcat agatattttc gccgtactga tcgtaaagca catcatctgt caaggagatt
    12721 tcatctagtt tttcctgcgt aagatttttt tctccgtcag caatgacttt ttccaatcct
    12781 ttttccggaa catccagttt ttcacgttct gctggatgat cttgcagttc ttctaattga
    12841 tcaaacacat acgacttatc cagctcgtaa gcataacgga aggaatcttg acgtaaaaca
    12901 gtacgagtac ttgtttcgcc atttgcatct ttgtattcaa tatcttgcgt gctgaacttt
    12961 cctgctggaa cgacaaaagt cagtatccat gcaaagatga ctacaccaaa aataatgaca
    13021 tacgtatggg gtgtcgataa tttagtgaag tcgcgtttcg gttttcctga agaatttgtt
    13081 ttcttcaaat ttatttcttc ctttcttttt tcagtaattt tgatgaataa aaaatggata
    13141 atcaaatgaa ttatacagta aaaatgacat ttccacatga ataatttatt agaaaatcac
    13201 ttttttctat tttgtttttt ttctcctatg tatgattatt tttttctctt tccatttata
    13261 ttaagtattt tatttctctg tttttcctta aaattctttt tttattttta gattagagaa
    13321 aatctcgtcc tgtttttttc attttacaat cattgaaaaa ttgaatttca ttgcaaaaaa
    13381 aataataaac acttataaat atccattatc tatccttcaa tttttgtgta ttttatttct
    13441 attagaaaaa ggtatcctgt tggacaattt tgttatatca ggtaaacttc attgcaataa
    13501 tgataacaaa tgtactataa ttactttgta aagagttttg gggaagactc tttcatcaac
    13561 gtgtattaag agacaagaaa gaatccatat ggattctttc ttgtctcttt ttccactact
    13621 ttttattttt ctgacactac atattcttat aactcatttc tcataactgt gaaacaactt
    13681 ataaaaataa caaactttaa acaaaacaag cttaaaatca aagatgatcc atcttaaaat
    13741 aactaaaaca aaaaaagtag tcgttgttaa tcctcattaa taatcgtttt ctttttttca
    13801 aacgctccat ttttatgtgg aacattattt ttttaaggaa ctttgtcaac atgtggataa
    13861 ctattttcca cttgtgtcct ggactagctg ttttgctgta aaatacaaaa cttgctgcag
    13921 caaaaagatc gttgcaagag aggctgttcc gcagatcgct ggatctagat ttggcaaagt
    13981 caagactgca tcatgtaaat tggcgtggtt gtccaaaacc aaatcagcaa attcaaataa
    14041 tcgcaaacca ctgctatgtt gcgatttgat acttcgtgac aaatcaaatc cagtaacaat
    14101 gattaatgga tgaccatttt ctttgatcca ctgagctagt tcgatgattg ccgggtttct
    14161 tccttcattc gaaagaagaa gaaaaatgtc ttctgatttg acagaaagct gatcaataat
    14221 tgcctgtcct gcaccttcta gcgtttcata gacacctccg gctggatttt caatcaatat
    14281 acttggtaca agatccgggg tttgatccca aaattcaaat gctacaccac ttagtgtgcg
    14341 gctagcatat atctgcaatc taccaccttg attaatttgt ttcgcgatcc aagtgccagc
    14401 ttgaagcatc agcggctctt ctatccttaa tttctcttcc aattggtgat aatagtcaaa
    14461 ataacataaa ctcatttatc tctcccgcct agctatactt gacatgaata gaaacagctg
    14521 cctaatctaa ggaagtacat aaaaaaacag tagttagaca gtaacacatg tggtctttgc
    14581 tgtttcaaac acaaaaaagg cattgaacga atacgcccaa taccttgaca tagcaaataa
    14641 aacaacgtgc agaccatgga aaatatctac ataggggtag aattttcacc ctagattagg
    14701 tacaataaat taggtcgatg cactgcattg actatggcaa aatgatcggt cagggtgtat
    14761 gtgtgtgact ggtcattccc tgattcttag cgccaactaa gggtcaggga gccttttttt
    14821 attcagctgt ttctatctga atttattgta agtaatctct tatccaagtg caagcaagaa
    14881 aatacaaaaa aacattggta tgatttatat atatataaat aacataaaaa aaggaatggc
    14941 ttatcccgat acaacaagca ttcctttaaa aaagattctt ttatataagt gaaaaaagct
    15001 tttttccacc aattaaataa atctctcatc tcaatagttt atccattatt ttatcttttt
    15061 ttgcgtatac ctactaaata ttaccgtctt cttctcgttc ttctactttg ttcgagagaa
    15121 tctgtactag aaaaagtagt tgtatgtcta ccttttctgc ctatccgaga ctttcttttc
    15181 atatatggga tataaatgat tagatacagt acagcaaaaa ataaagcaaa tacgcaagct
    15241 tcccaactac ctaacgccaa ttcaaccatc aaggtagcaa ccccaacgga tgctgcatac
    15301 cagataaaaa tcagaaacgt ttcttttttt ctgaccatgc gaaacatgat aaatagacta
    15361 ataagcaata gtggcaacac aaatatccaa ttgcttcctt ccatcagcca cttgagtata
    15421 tcaagcagcg actgtgcaac agcgactaat tttcccatag tgaacacaca ccttttttta
    15481 taattaaaaa attcgctttt ttattctact atgaatcttc tggcttggct ttcatttctc
    15541 ttatgtaatc attttctttt atacaactta aaaaaaaatt aaaccattag cataatattt
    15601 tctgaatcta ttcacgaaag tctttttcta tctttcgttt tccacgactc aactgatcta
    15661 gagtcaaaaa actaaaacga cagttgcgca aatgacgcac aagctgccgc tttaataaca
    15721 aatagccttt cattctcagc aggccactta tttccagtta actttctgtt cttagctttt
    15781 tactcaactg gataatatat gcacttaatg aagtcccaat ttccgaatgt tgaagtccat
    15841 attcaatatt tgtcttcata tatcctaact tatcgcctac gtcgtatctt tttcctttaa
    15901 actctctcgc atagaccgcc tctgtctggt taagagtctg aagtgcatcc gttagctgta
    15961 tctcgtttcc tgctcctggt tcttgagttt ctaatatctc aaatatttta ggagtcagta
    16021 gataacgtcc gataatggct aaattacttg gtgcttttcc catttcaggt ttttctacaa
    16081 acccttttac tctgcaacga cccttttcgc tatctgtttt aggatcaata attccgtatt
    16141 tagatgtttc attttctggt acacgcatga cggcaacagc ggaagcttgt gtttcttcat
    16201 aatccatcat caattgttga gtcaatggga tatcatcttt catcaaatca tcccccagca
    16261 tgacgacaaa cggttcattt cctataaatg cttttgcttg aagcacggca tcccccagtc
    16321 ctcttggatg agattgacgg atgaagtgaa gattgacttg agtcgtttct tcaactaaag
    16381 cgagtaattc tgttttttcc ttttcccgca aattgttttc caattcgata tttgcatcaa
    16441 agtgatcttc aatgggtctt ttttcttttc ccgttacgat caagatgtct tcgattcccg
    16501 aatccaatgc ttcttccaca ataaactgga tcgttggttt atccacgatt ggaagcattt
    16561 cttttgccat ggcttttgtt gccggcaaaa aacgtgttcc tagcccagcg gcaggaatga
    16621 ctgctttttt gaccttcact t
//
LOCUS       DACGLW010000068        16639 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.071, whole genome shotgun sequence.
ACCESSION   DACGLW010000068 DACGLW010000000
VERSION     DACGLW010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16639
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.071"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            179..595
                     /locus_tag="IXT53_002129"
     CDS             179..595
                     /locus_tag="IXT53_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905926.1"
                     /translation="MQHRQIRLYAGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPE
                     IHHYTSDKKILIISDLSLRHSQYIEEYISDILAVHKIHSETTAITEDQLSKYNLLEYD
                     LIVTNQPILNAHVPHILIDDSVSFANEEELIRLFEL"
     gene            complement(668..1315)
                     /locus_tag="IXT53_002130"
     CDS             complement(668..1315)
                     /locus_tag="IXT53_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ4905927.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEKLLKDFRYKSRWIAEGIQ"
     gene            complement(1323..2009)
                     /locus_tag="IXT53_002131"
     CDS             complement(1323..2009)
                     /locus_tag="IXT53_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4905928.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(2015..3088)
                     /locus_tag="IXT53_002132"
     CDS             complement(2015..3088)
                     /locus_tag="IXT53_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HAQ4905929.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            3310..3984
                     /locus_tag="IXT53_002133"
     CDS             3310..3984
                     /locus_tag="IXT53_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ4905930.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            3989..5329
                     /locus_tag="IXT53_002134"
     CDS             3989..5329
                     /locus_tag="IXT53_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ4905931.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(5478..6464)
                     /locus_tag="IXT53_002135"
     CDS             complement(5478..6464)
                     /locus_tag="IXT53_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HAQ4905932.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            7188..8219
                     /locus_tag="IXT53_002136"
     CDS             7188..8219
                     /locus_tag="IXT53_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ4905933.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            8328..9860
                     /locus_tag="IXT53_002137"
     CDS             8328..9860
                     /locus_tag="IXT53_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HAQ4905934.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            9999..10811
                     /locus_tag="IXT53_002138"
     CDS             9999..10811
                     /locus_tag="IXT53_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HAQ4905935.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            10801..11286
                     /locus_tag="IXT53_002139"
     CDS             10801..11286
                     /locus_tag="IXT53_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ4905936.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            11309..11854
                     /locus_tag="IXT53_002140"
     CDS             11309..11854
                     /locus_tag="IXT53_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HAQ4905937.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(11927..13570)
                     /locus_tag="IXT53_002141"
     CDS             complement(11927..13570)
                     /locus_tag="IXT53_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HAQ4905938.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(13699..15927)
                     /locus_tag="IXT53_002142"
     CDS             complement(13699..15927)
                     /locus_tag="IXT53_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HAQ4905939.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            complement(15960..16439)
                     /locus_tag="IXT53_002143"
     CDS             complement(15960..16439)
                     /locus_tag="IXT53_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905940.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
BASE COUNT         4933 a         3176 c         2995 g         5535 t
ORIGIN      
        1 tgaaatcagt gagcagatac gaaaaaatat gtcctactgt tcgttttcat ccgctgattg
       61 caaagaaaaa aacgaagaat atgttgttca attattaacg caaatttatt ttttctcccg
      121 tattttccca tacgatacga ctcatttgac gatccgtgca acttactttg cacagcagat
      181 gcaacaccgc cagatacgtc tatatgctgg atttcgtgca gaacttcaat tttattccac
      241 tctactgaat cagaatctga tttattattt atctagctat atcttttgga tgcttatcgg
      301 taatcctgaa attcaccatt atacttctga taaaaaaata ctgattatca gcgacttaag
      361 tcttcgacat agtcaatata ttgaagaata tatcagtgat attcttgctg tgcataaaat
      421 tcactctgag acaactgcta ttactgaaga tcagctgtcg aaatacaatc ttctcgagta
      481 tgatctgatc gttacgaatc aacccatttt gaatgcccat gttcctcata tattgattga
      541 tgatagtgtg tcattcgcaa atgaagaaga actcatccgc ttattcgagt tatgaataat
      601 caaaaaacag ctatgtataa accaccctga taaaaatttc aggacaaata cacatagctg
      661 tttcttttta ttgtattcct tcagcaatcc aacgagattt atagcgaaag tcttttaaca
      721 acttttcttt tgtccaatta tttgcaatga gatccgtcaa agtgttcatt acgagggtca
      781 tggaaaagta taggaaacta actgcttctt ccgacgattg aatggagaaa tgattgattc
      841 ttaataattc cagccatttt tcgttcattg gagaatcagc cgatagttct ctacgtttgg
      901 ttgtctgctg atcatttcgc tgggttaaca agtataacct ttgccaatag atactttcgt
      961 gatctaagtc gcagcaccac tctaaatatt tttcgatccc caaaaagaaa tgttgtttgt
     1021 tttcgctaat gaattttaag atatcttgat ggactatgtg agaactgctt ttgatcgtat
     1081 acaaataagc atcttccaaa tcttgaaaat atttataaaa agcaccgcgc gacatcccca
     1141 tcttgtcaac gatttctgag actttgactt ggctgatgtg ctgtgagcaa aatatctcca
     1201 gtaataggtc atcgattctc tgtttctttg cgttcgataa attgaaaaaa gtttcttttg
     1261 gcatccacat ctctccttta tctctgatta tacgtggata gcccttcatt gtcatgaagg
     1321 attcattcct tctttttctc ttcttctgtc aagtacccat cttccattcg ataaacgcga
     1381 tcactccatt gaatcatacg gacatcatga gtaaccataa tagtcgcttt ttgtttctca
     1441 tgagcttctt taactagtaa tttgacgact tcatatgcat gatctgtatc caaactagct
     1501 gttggttcgt ctgctaagat caagcttggc tcattatata aagcgcgagc aattgccact
     1561 ctctgtcttt ctccaccaga aaggtcttta ggataactat ttttaagtga ctggatatct
     1621 aaagattgca gcaaggaatc gatttttgtt gaatcttctt tttttgaagt aacttggtca
     1681 actagttcaa attgttcctt taccgttaaa aaaggaatca aattagagct ttggagaata
     1741 aatcctattt ctttaaagcg taaatctgct cgtttttttt ctggaagctc agttagtgag
     1801 tgaccgttga tcactatttc tccggaagtc ggtgtttgca atcctcctga aatggtaagg
     1861 aaagtacttt tacctgaacc agaaggacca atgatgctga cgaattctcc ttcctttaca
     1921 taaaaatcaa ttgctttcaa agctgagacc tttgtatgac cagaaccaaa tgttttggta
     1981 acttttttca tcgttaatat ttttttcatt tttattaacc tcctattgct gtaattgggt
     2041 ctactttggt tatcgtacgt atcgaaaaca atccgcctag cattgcgaca atgatcaaaa
     2101 tcccactata tagcaaccat tgtgaccaaa aaacagcaaa aggcatcgcg cttggtaaaa
     2161 tcagactagt caaataagca aacaaaagag caagcagaac gcctaccacc ccaacaataa
     2221 aggactgtgc tactaaagat ttagcgatga agaaattttt gatcccttgt gctttcatta
     2281 ctccaaaaat agcagtcttc tgaagtgtca tcacgtacat gaaaataccg accacggcag
     2341 caaccactaa gaacaaaaag tatatcatcg catttaaggt catattttgg gcagaatagc
     2401 caggaatact ttcaatgaat gtcccaatag ttaataactg tagacccttc gatgcttttt
     2461 ctttcgaaat tttagctgaa tcctttatta cgatcccatt gatcggctgt tcttcttcag
     2521 aagcaaacgg ctgttgtcca tacttcaaag cagtccacgt atctagggac gcatagatga
     2581 cgggagaaac tgtgtaataa gtttcaggaa agataccgac aatcgtcaaa ggatcgtcgt
     2641 aactgccaat tttgatcgta tctccaattt tatagcctga atcagccaga ttttgagaga
     2701 tgatgatttc attctttgtt ttaaattcat gccctttcgt gagttttggt aataaaaatg
     2761 cttctttcgt tgtgccgaaa actgtaacat tttctttgtt ttttcctttg attgctccgc
     2821 tgtacaatcc gattggtgct ttctctcctc cttcaatgta tttcagatct cctcttgtta
     2881 attgtgaagc tgcaaatact tgatttgcat cctccgacaa aacaatctct tgtgcgtccc
     2941 aatcatcgat tgctttttta aactcttctg aaagtccatt cgctagtcct gacaacatga
     3001 atacgacata cgcaatcaag aacataatac cgacgatcaa gccatatcgt aatttagaat
     3061 agcgaatttc tttgatagct agaaacattt gtttttcctc cctatgatag attgatagtg
     3121 acaaaatgtc acttgttgaa tagtgtaact ctaaagtgac aatttgtcac ttgtgttgtt
     3181 tttttcttat caataaattg tctaagacat ctttcgataa aaccttaaca tgagtcataa
     3241 aaaatttatt taagccttat ttatgctttc catgataaac taaagtgacc aaatcaatga
     3301 ggtgggaaaa tgattaaact attagtagta gaagacgata aagtattatc caataatata
     3361 aaagaaatac tcgctgcaat cggcgagata acacaagttt atgatggtag tgaaggactg
     3421 ttcgagttgg aaagtggagt ttatgatctt gcaatcttgg atttgatgat gccggaaatg
     3481 aatggatatg aagtgttagc aaaaatacgg aaagaaaaaa tacagactcc agtattgatt
     3541 ttgactgcaa aagatagttt ggaagataag attgaaggat ttgaacgagg tgcagatgat
     3601 tatctaacaa aacctttcta tcgagaagaa ctgatcatgc gcgtgaaagc attattgaaa
     3661 cgctcgctag gtctctttga tgaaaatgtt ctgaagtata aggatatcag ttgtgattta
     3721 aaaagcaatg aagtcttgta taaagaacaa gttttgccca tacaaggaaa agagtttgaa
     3781 ttgctcgttt attttttaca aaataaggga atcattttga caaaagagca gatatttgac
     3841 cggatctggg gatttgattc tgatacgacg attacagtta ttgaagttta tatgagcaac
     3901 ttacgaaaac atctgcgaag tactggattg gataaagaaa tcaaaacctt acgtaatgtc
     3961 ggttatatac tgcaaagggt gtgatgcaat gaatggagat attacacgaa agcaacgtag
     4021 tcgctttttt gcgacaaatg tagcagcatt tgcttttatc ttttttcttt tgggattcat
     4081 tactttacag atattaaacc aatctgcata cagagaaaca gatctgtcat tagagaaaat
     4141 aaattttgat tctcgaacag tacaattaga gatacaaaga tatcagcaag gcgatccctt
     4201 tttaaacccg actcaaacgg aacaaaacag aacaaacatg gaacagccat taatggaacc
     4261 atcaaatgac cgttttaaca ctcaagtgat cttgtggtct aaaaatggtg agatattgaa
     4321 taaagcagct attggtgggc gtttcaacca aatcaaagaa ttaaaaatgg atacgaaaaa
     4381 tctgaatacc atccaaaata ttgaattaaa tgaagacagt ggctcatctg ccctctcttt
     4441 ccgttcgatt acgaaaaaag cggagacgaa tcaagatggc gttgcctaca tccaaatttt
     4501 agaaaataca aatcaaatca agaattcatt agcgactttt cgcacgatta tgattttgtg
     4561 tatgattgtt ttttggattc tctctattgg tatcagttat tacttgtcta gtctaagcat
     4621 gcgccctatt attctttctt ggagaagaca aaaagaattt gttgagaatg cttctcatga
     4681 attgcgcaca ccgttaacga ttattcaaaa tagtttggaa catcttttta ccaagcctaa
     4741 tcatactatt attgaagaat ccgaaagcat cgctcaagca ttgtcagaga cgaggcgcct
     4801 cactggattg acttcggatt tgctgacaat cgctcggaat gattctgatc agcagctgct
     4861 ttcaaagcaa atgatcaata cacaagaata tatcgaacaa cttgttaaac catttcaaga
     4921 gatggccatc atagatggaa aagagtttat tctccataac gatgcaacat tagaagtacc
     4981 aattgatgaa aaaaaaatcc atcaagtttt ggttattttg ctggataatg cattaaaata
     5041 tacgaatcca gaagataaga tcattttgga ttcatctgtt aaccataaag aatggctgat
     5101 tcacgtaaag aatactggcc cttctatcag tgaagaagat caagcaagga tttttgatcg
     5161 attttatcgg gaagatcaat ctcgggcgaa agagactggc ggatacgggc ttggcttggc
     5221 aattgcaaaa caggtaatcg aacagcatca tggacagatt tctgtatcca attatcttcc
     5281 acaaggagtc gattttaaag tccaattgcc gttgaagcct aaaaaataat gcttatccct
     5341 aaaaaagggc tgtgacaaaa atcttgtcac agcccttttt tgttgcgaat aaacggtgtt
     5401 ccagaagtag cttcttcgga aataagccga aatttcttta agctgaccac ttctgtcaca
     5461 acctctttag cttacgctta cgatttttta ttgcggtctt caatgtgtac atcaccgctt
     5521 gtgtcaatat ccaattcttc tcggctaaca tcttctgtca ctttttcaac atcttcatgt
     5581 tcttctttat gaatcccaac ttcttcacga ataactgtat gtttgcttac atctacttgc
     5641 tcttctttga ttgggatagt aatcgtgtca tcttccaatt tttcgtctgt tccctgtgaa
     5701 gattgatctg ttactggttt gcgttcgatg acgatttctt cacgtttgac cggtacttct
     5761 accgtttcag tatcgttgac cacatgtttg tggatgtcga cttctccagt tgtcacatca
     5821 tgtgtattta catctaattt ttcttctttt agttgaattg tatcatcatc tagatttggt
     5881 ttgcgatcga tgtcttcagt cttgcttgta cccattgtat cagtctcgcc aggtacgtcc
     5941 atcggatcag ttcctggtgt catattcaat ggatccgttg tattttgaaa gcctgtcgtg
     6001 ccacctacgt ccattggatc tgttccagtg ccagtttcta atccagtccc cattgtgctt
     6061 gctgggctaa cagtatcttg tgtatccatt gtatcttctg ttgtttctgg ctgacgatag
     6121 ccttttactg tgatgacaaa ttttccatca gaaatatctc ctctatattg atataaagga
     6181 tcattttctg gtgtagccgt ctcagaatca taggtatcaa aagaaaaagt atcttttatt
     6241 ttttcccata aagaacggtc ttcttcacca tccatccgat tttcatctgt ttcaacatct
     6301 ataccagaca atccgtcaaa tcctagtcta gatgctgctt catcagtagt ataaataacg
     6361 atatcatctc gttcataccc ttcgttacgc aaccgttcaa ttactgataa tgcttcttcc
     6421 ctcgtactat agcttccgat gaccgttaaa tttttctctt ccatgtaaat tcctcctagc
     6481 aaatagattt atgattggta tattgttact tataaattta ttctatagga gacgattgtt
     6541 ttctacaaat atttagattg gaatttgatt aaaacaattt aattgaaatt tgaaaacatt
     6601 tatgttaatg aaagtcatat tctaataaaa tgtcatttgt ttctatatga atttttatga
     6661 aaaatttcat ataatttcca ctcgttgtga tggtttaatt tatccaatcc atcaataagc
     6721 aaacaactta tataaaaaag gattaattct caatataatt gctcctacca tagaataaat
     6781 cttcatgtaa agtaatgaag taccaactat ttctaatact tctttaagaa ttatcccaac
     6841 aatatcgtta gacaaaccat tattgaataa atggagagcc aatcgattct gaaaaaatgt
     6901 tttctaattt ttcggaaata aaagaaagag cctcctgcca ttagaacgaa ccaaatccat
     6961 ttcattgctc atgaagtaac cacatagcta aaaagaggat aaatcaatta aaagatcaaa
     7021 agaatgactg cactaataaa ataactaaaa atgtatcttc ttacttcata tcttttagct
     7081 agtacttagt taaaaatttg cttcctaaga tcaagacaaa ctgtctatct tcatttgatg
     7141 aaatgcggta aaatatagaa aatgaacact attggaggga atttgtaatg gacagcaaaa
     7201 ctagaaaaca actgcaagaa aagttaaagc aacgtacaca cttgctccct tccgtctttc
     7261 aacagttttt agaagaaaat cgttcttccc tttcattagg cagtcgctat gaatacgcaa
     7321 aagattttca tctgtttact gattatttga gaaaagtatt ttctgaagaa gtggatgatc
     7381 agctgattat ccatttagaa aaacaagatt atcagaattt cttagctaaa atctatcgac
     7441 atactcgttc gttccaaact acagcgaacc aagaaacgga gcaattgttt gaaaatagct
     7501 attacgggat ttcaaggaag caatattcat taaatcattt tttacgattt ttgtatcaaa
     7561 aaggactgat tgaagaagag atttctcttt tgggtacaag tttaccggaa acaaccaaag
     7621 cagctccgcg atatatcgat gcagaacttt tcaaagattt tgagcatttg tttatcccga
     7681 ttgaaggctt tcaaacaagc agagaagcta gctttgaaga aaagaataga ccgaggaacc
     7741 ttgcaattac tgccattctt ctgtattgcg gattaaagat ccatgaagta gtagaactta
     7801 aacgtaaaga tgtatctttg accaagcaga ttcttaatct gaatagaaaa gaaaacagac
     7861 tgaataaagt accaatacca aaagaagcga tgccatttct ggagaactat tttaagttaa
     7921 cagcacaaaa tacggacgaa aattcttttg tttttcgttc ctcacatgat cagcaaattt
     7981 ctcctcggac tattcgccaa attttgagca aaattacagt atataaaaac gtatcgccag
     8041 aactatgcag aaaaacatat cgttattatg cagaactata tttagatgat gctgacgcgg
     8101 taaattattt atgtggtaat cgatacctct catcccattc ttttcaagat atctgggaaa
     8161 agatggttga tttttcttat cacgaattag cggcattcgt tcgggtttct tccatataaa
     8221 acaaatgtat gatctttttt tcacaataaa ctatgttatt atataaagga ttatgttacg
     8281 attatcttga atttgtatat tacttaacaa aagataatgg agtgtgaatg atggagacca
     8341 taacgtattc ccctttgaac ttatttacga attttcaaga agctgcagct aacttttccg
     8401 agaccccgat tatctttgat gagccacttt cagctttccc agaattagga cttgaaacaa
     8461 cttacaaaaa atgctgcgaa agtgtgttga aacgggctta tcaattggct catcttggag
     8521 tgaaatccgg agacaaagtt attatttata agagttccgc ttttgacaca taccttctag
     8581 ctgtttctgt gtcttacctt ggtgcagtgc ctgtcatgac ttcttatcat ctaccaacaa
     8641 caacaatgga agtatttatc gatcgtttag aagacccttt cattttattt gatgatgaga
     8701 caaaaaagcg agtgagagaa atttcaaatg gaacaaaatc aaaacagatt cctattcttt
     8761 atttaatgga acaacctgct ttgtctgttt ctcaatcatt tttagacaaa aatgaaattt
     8821 cttatatgac ccatacttct ggtacaactg gtatccctaa acttatttgt cattctgctc
     8881 attctatggg atggcggacg aaatggcaaa aaacagtttt cacaaaaatt tcggaaaaga
     8941 aattaatcgc ttttcacatc tctccagtgc attccagatt caatattggt atctcttctt
     9001 tgatgtccat gggatttcct atgatgcctt tagcaaacgc acaaagttca aaagttgttc
     9061 atatgttgga agctcaccgg ccaattgcac tagagaccca tccgaataac tttgttcagt
     9121 ggcgttttac tgctaaggag catccagaag ctttttcggg tatccgatac tatcactcca
     9181 cttttgatgc tataaacaac cagacaatga agacattttt gcgaacgtcg atggcacagg
     9241 atgcggtatt tcttcaagta tatggccaaa gtgaatgcgg accaatgatt cttaaggctc
     9301 atacactaga gagtttgaaa acaagtgatg caagagatat gggcgttgga ctggaagaca
     9361 tgacaagcgc tcggattaca gattcagttg ggaatgtcct acctgaaaac acagatggtc
     9421 atatacaatt actgtctaaa ggacgggctt tgacttatta caaggaagat gcacggttcc
     9481 aggaaaatgt ttatggcgat tggtgggata gcggagatta cggttttatg gataagcatg
     9541 gccacctatt tttgaaagat cggcaagtgg atttgatcga aacgatcaat agtacattag
     9601 ctattgaaga ctttttatta gattcgttag attttttggc agaagtagtg attgtaaggg
     9661 ataaaaatca ttctccacaa ccgattattg ctttagctcc tgataaagaa atggattgga
     9721 atcgatggtg ggagcaagtc catgaacttc ctcgcttgaa cgtccctatc atccgtgatt
     9781 ttgatgcaat cccgcgaaca gctacaatga aagtgcaacg gcttcaaatc gagaaagaat
     9841 taaaatcaca aaagtcctaa ctatttaagg aaagaaagat ccattttggt ctttcttttc
     9901 tttttgtttt tttaaattgg atgagataaa aatatgccaa ttggctgttt ttcttttttg
     9961 aaaatttcgt tatactctta ttcatggagg aaaatgttat gaaaagaatc ttgacaattg
    10021 gcggttctga tccatttgct ggtggaggaa tccaaacaga tttaaaaact tttgagaatt
    10081 ttggtctatt cggactaagc gccttgactt ctataggggc actagatatt aatgagcgat
    10141 ttatgctgga atctatatct ttggaattgc ttaaacgaca gttagtttcg attgataaaa
    10201 tgacttcatt agatggtatc aaaattgggt tattgaattc aagtgaagca atcatggttg
    10261 ttcgagactt cataaaaaca aaacaaaata tcccaatcat tttagaccct gttttggcat
    10321 tcaaagaaac tgaagctgtg atcaatcaat tgtatatttc gcagctcatc gaagaacttt
    10381 ttccacttgt tacgatgatt acgcctaatt tggcagaagc tgctttactt tctggtcaag
    10441 ctcttccctc ttcgttaaca gaaatgatcc atctcgctaa agaactcact gcttcaggaa
    10501 caaaaaacat tgtgatcaaa ggcggcacaa ggatggctgg agatgaagct gtggatcttc
    10561 tctattcaat tgatcattat gaggtatttt ctcataaaaa aatcgacact acaacagtaa
    10621 acggggccgg ttgtgccttt tcttctgcaa taacagcgaa tcttgtgtta ggggcagatt
    10681 ttaccaaagc agtagcagaa agtaaaaaat ttgttgccca ttgtatttct aatggcgtgc
    10741 tgatgaacga tcataccgga agtgtctggt ctggcggtat tcaaaaagga ggaatgagaa
    10801 atgaaaacta agcaattaac gatctatgct atgctgattg cgttgacagt tgccctttca
    10861 ttaacaattt tgatccctgt accggctacg aatggatttg tgacattatg cgaggccggg
    10921 atctataccg ctgcttttct ttatggtcca acgggtggat tagttgttgg tgcttccagt
    10981 ggtctattga tcgatttgat ttcaggatat ccgcagtggg cagttttttc attagtgatc
    11041 cacgggttac aaggatatgt tgctggttct ttcagcaaat cttctactcg tatgtgggga
    11101 ttagggcttt tattaggaag cttagttatg atcatcggct atggtatcgc cggttggatt
    11161 ttatatgatt ggccggctgg agtggcttct ataccaggaa acatcttcca aaatctgatt
    11221 ggaatgggac ttgcctttcc tataactgca agtcttcaac gtattcaagc acggaagcaa
    11281 tattaattga aaaagaaggt tttagaaaat gaagttgtca gaaaaagaat taaaagacca
    11341 attgactgaa atagttaatg acatattggc tgaggcacac ttgaaaaaag gtgatctttt
    11401 tgttttagga tgcagcacaa gcgaagtcgt tggaggacat attggaaaaa attccagtgc
    11461 ggaagttggt caatggatca tccgtacgtt aaaagagttg ttggatccta aagaaattgc
    11521 gctagctgtc caaggttgtg aacatttgaa ccgagcatta gtagtagaac gaacagtggc
    11581 agaagcaaaa aatttcgaaa ttgtctctgt tgttcctgca ctacatgctg gaggtgcttg
    11641 ttctgttgct gcttttgacc agttcaatga tcctgtcgaa gtggaacacg tagttggaca
    11701 agccggtatc gatatcggtg acacatcaat cggtatgcat atcaagcatg tccaagtacc
    11761 tgtcagacct cgtttgaaaa cccttggaca agctcacgtg acagcccttc gctctcgtcc
    11821 taaatatatc ggtggtccta gagcgaatta ttgaaatgag taattgacag agcaatctga
    11881 aaaacttcct cgcaaagtcc aactaatgaa ctttaacgag gaagttttac ttgtttgttt
    11941 tatataaccg agcttcataa ggtgctagta tgcctttagc ttcaaattct ccgctagagg
    12001 aatttgacaa gagtaatgtc caggactgat ctgcttcttt tggaagttca tagtcagcaa
    12061 gtgattctgt taagtttacc ataataagat attgttcatc ttctaaatat ctttcataga
    12121 cataaagttg tggatgttca tgaagatatt ctttataaga accgtatatc agacctttat
    12181 tggtttgtct aatgcggatc atgttcttat agaattgcag gaccgactct ggttcatatt
    12241 cttgttgttc tacattgatc gttttggtat ttggattagt gatcagccaa ggttcatgtg
    12301 tagaaaaacc agcatattgt tccgaagtcc attgcatggg tgtcctgctg ttgtctctcg
    12361 ttgtttcacg aatgatatcc aatgcttctt ctcttgtctt accttccgca agcagccttt
    12421 tataaagtct tttcgaatct actgcatcaa cttgatcgat cgattcaaag ggcatattcg
    12481 tcattccgat ttcctgtcct tgataaataa atggcgtacc ttgcaaaaag aaatacatca
    12541 tagcgattgc ttttgcagac atagcccaaa aatcgggacg agtatctcca aacactgaaa
    12601 cggcacgagg aacatcatgg ttttccatat ataaagcgtt ccatccgata ccatccaatg
    12661 aagtttgcca agcgcttaat gcatgtttca atttaaggac atctaattgc ccttcttttt
    12721 cttgctgcca gatatggata tgatcaaatt cgaatatcat atcgaaataa ccgtctttcc
    12781 ctacccattg tggtccttct tctgcagtga caccacttgc ttctccgaca gtcatgatat
    12841 cgtactcttc aaaaacatgt ttcaaatcgg tcaaatgttc ttcgattcct gagacatttt
    12901 gaaagggtga gaaagggttc tctgtggctt ttactgaata ttcatctttt tttacatgag
    12961 aaatcgcatc taaacgaaat ccatctatcc ctttatccaa ccaccatcgg atcatattga
    13021 atagttcttc tttcaatttc tcgctttccc aatttaagtc tggttgttct tttgcaaaaa
    13081 catgcagata atactgtcca gtttcatgag aatactccca agcagaaccg ccgaaaatcg
    13141 atgtccaatt attcggttct ttgccatcta ccccgtcgac ccaaatgtaa taatcgcgat
    13201 acgggctttc tttagatttt ttcgattctt caaaccattg atgctgatcc gaactatggt
    13261 tgatgacaag atccataatc actttgatcc ctaattcatg tgcttctttt agcaataaat
    13321 caaaatcttc catcgttccg aatctctctt ctatatcttg atagtcactt acgtcataac
    13381 cattatcgac aaatggagat tgatagatcg gattgatcca gataaagccg ataccaagat
    13441 ctttcaaata attaagtttt tcacggatcc cattgatgtc tccttgacca tcgccattgc
    13501 tatctttgaa actagcagga tagatttgat agcctactgc ttctttccac catttttgtt
    13561 cttgtatcat atcgtttcac tcctttaacg gtccctcttg attataaccg attctatttt
    13621 agaagtatcc ttgttaccgg taactagaaa cgaaaagctc aacgaatctg ctaagtgcgt
    13681 tcgttgagct ttattttatc atagatagcg ggcaatcagt tgaatcattt gcttgggaaa
    13741 ttcattcaag tcagtaatat ctacaaaacg actttcaccg tagatcgact tgatttcttc
    13801 tttgtcttgg ccaatagctg cggagacaaa taggattcct tgacgttcat attctttcaa
    13861 aacagactgg atatcttctt ttgctttttt gcctgtataa tccggcaacg cttttggctg
    13921 accgtcacta atattaagca acagtttggt tgttgcggat tgtctgttta atttttctgc
    13981 tgctaaacga agtgcagcac catcacgatt gttttgcctt ggtttcatag tgactaattt
    14041 ttcatttaac cattgaaatc cgtcttcaaa ttctttataa gaaaacaaag aagtcttttc
    14101 tcttgacgaa cggtctgctg tatctccgta aatcaacaat gggatatcga ctttcttaga
    14161 aaattcggca atggcaatcg ctgctttttt tgccgcttcg atccgatcat cccgtatcat
    14221 tgacccagat tcatcgatcc gtacagctac cgctaaagaa ggttgctcat gtggcgggtt
    14281 ctttttgtca aaatttctca aatcaccata tgctactttg cttgcattga accgctgacc
    14341 ttcgtatttt cctttttggt acgtctctgt ttgttcatta tccagtaact ctaaaatttg
    14401 ccggctaagt gattctacta ttggcattac ttgcatgtgt agctcacgtg cttccggcgt
    14461 ttttccttgg aaagctggtc ggtgaacgat gagaccttct ttttcatgaa tcgtttcctt
    14521 catgatttgt cgcgcttctt tattcagttg cttcttgatt tccttatcat gtgcttccat
    14581 gtcagaagca gacatttctt gagaatcatc tcctgtttgt tgttcttttt gattttttcc
    14641 ttcgatatcg ctactgcgtt ctttttgcat ttgaggagga atctcgtttt tttctattgt
    14701 ttcttcttgt ttctcttctc cctcatttgg ttcttcagca gacaacgatt tcttttcatc
    14761 taagcgcaga atttcttcag cattttcagt gatgtttcgt tcatttgctt cttcggcgct
    14821 aatggttctt tcttttgtat gctcagattg aggatgaccc catttttctt ttctcaatat
    14881 gtcctgaagt tccagtagca atccactgtc agacaaagct tgttcaagaa ttgccaattc
    14941 atctgaatca gtggataatt tatacaaaac ttttggataa atcgactgga gtaaatcttg
    15001 tccttgctta taggtatttg cccaataaaa ataagaacgc attcctgcta cacctttgat
    15061 ggcgttagct tttgcagttt catccaacaa acggataatc tctgccatct ttaagagaag
    15121 ttcttgtttt tggatcatag ttttcgccat cgcacgttct gccatgactt ctaaggcagg
    15181 aagatccatt ttttccgcat gttgcatccg atctcttaaa gattcattta atgggcgatt
    15241 tccttggtaa tttcgatttg tagtgatcac aatgacgcaa tctggatggc gacgaatgat
    15301 tcctgtcggt aaattcaaca tcccatttgg ttcgagcgct gagttaagtg ccacaagaac
    15361 agaggcatct cggatgacag tcggctcttg aatctctaat aaatagcctt tttcaatcgc
    15421 tcgaacgatt tcagaagggt aatagctgta atggattgca tctggataat cagcttcgat
    15481 cctttgaact agctctgcta atttttcttt agcagaaaaa ggatcaatgc cgtaatggtt
    15541 ctggatcagt gtcagtacag cttcaagtgt atctgtttgg taaatagctt ctagtaattc
    15601 ttggtcttgt tccttattag aatctactac aggtaataag gctccgaaaa catctgattt
    15661 gtccatatct gcaaaacagg tcacttttgt atagggaagt tgcagatcag cagatagtgc
    15721 tttggctaat tgtgtcttcc ccgacccggc atctccttcc aaaagtacgt tactgatttt
    15781 catttcagga tcttgccaat tccctttgat ttcggaggcg atccgccatt cttctgcact
    15841 tgtccgatgg cttttcggtt tcttccagat catttgcttt tctagccgtg aaaaggttcg
    15901 gttttctgtt agtgagaaat tctccatgct tctcttcccc ttttgatcat ctctgtctat
    15961 taatatccta gctcatctaa aagacgtttt aaaatacgga atcgttcccc aatcagttct
    16021 ccgtcttttt gtagtgtatc gtaatacaaa atgatgtctt catcgatctt tggatttttg
    16081 gtatctactt ctaccgtcat tcggttccct tgtaatccaa tgatgtctac gactggattt
    16141 tctggatcat aaaatttttc ataacgataa gcatcttgga agtataaact tccttgcgca
    16201 gctaagatcg ctaaatctaa aatacgtgtg attggtaatt cttccgactg tcgtgaccat
    16261 ttttctccag tatgtcgcca taccttgcct gagatatcga cttttccacg atcattccac
    16321 tgagcaaggc ccagtgaaag cccttttgca tccatattta atgcatttct tccatcaact
    16381 ttatcatagt cttctacaac gattactggt ttatgtttta aatttgttgg tatttccatt
    16441 attttaactc ctcatccttt tatttttagt aaataagtaa attagtagtt tactcattta
    16501 ctaaactata tgcgacatta tagaggattg aagtgaggat tgcaaagaaa aagagcttca
    16561 ttgatagaaa taaaaacacc atctcgttct tttacacgct cattagtaac atgtagaaag
    16621 tccaagatga tgttggaat
//
LOCUS       DACGLW010000069        15960 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.072, whole genome shotgun sequence.
ACCESSION   DACGLW010000069 DACGLW010000000
VERSION     DACGLW010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15960
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.072"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            complement(<1..450)
                     /locus_tag="IXT53_002144"
     CDS             complement(<1..450)
                     /locus_tag="IXT53_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ4905941.1"
                     /translation="MKYKEMFVPSLIQLAVEVKNKEEAFLIAAQQLEKMGYVNENYLN
                     GLIAREREFPTGLITKYLNIALPHSECEYVEKPFVFVMQLKNPIYFYQMGDNQEMTVE
                     NLFFLGIKEPKEQVGLLQAFMNLFMDETFVENYKKQETPQNIYELITN"
     gene            674..1465
                     /locus_tag="IXT53_002145"
     CDS             674..1465
                     /locus_tag="IXT53_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ4905942.1"
                     /translation="MLKKERQEQIITLLEKDFYLTNSEIANKLGVSEMTIRRDVTELA
                     KKNKVKKLYGGVERKEPYNRELSTQEKIDTNVAEKKYIGQVMNSMIPDNSTIYVGAGT
                     TILYALPFIQKKNLFVITNSLIAFNYLKNHTSYRILLTGGEFSPITEEFIGEVAERSF
                     ESLNVDISFAATNGIFDNNVTTAQFIEGNIQNAALNAAKIKCIVADYSKLNKSDVYTF
                     RKLTDFDYLITDNHLSEQDFNHYSKYLTILKEPKNDTDNHDEPIH"
     gene            1431..2384
                     /locus_tag="IXT53_002146"
     CDS             1431..2384
                     /locus_tag="IXT53_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase family hexose kinase"
                     /protein_id="HAQ4905943.1"
                     /translation="MILTITMNPSIDSIYFTKHFELGTMTRFDSPVRYVGGKGINCGR
                     ASALLGSSVILTGFLGGYFGSMIQDMLKNENLFDLHFLPIAEESRGAVTIMHDDETQT
                     ELVEAGPEIADTTIKKLMNDIKLISKNNYLSVITINGSVNNPDPFLYAKLLQYIKEQI
                     DPSTYILMDISGQHLREVLASKYKPMFIKPNIHEFSDIIHQKITQKSDIKHHLKTNPL
                     FKDIDVIMISCGSYGAVVKVKEEFWNVEIPKIPIINTTGSGDATVGGFAHSLEQNYPL
                     KECVRYAMACGMSNAQQSEVGKVIPADVKNLAKSIKITEMK"
     gene            complement(2782..3756)
                     /locus_tag="IXT53_002147"
     CDS             complement(2782..3756)
                     /locus_tag="IXT53_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ4905944.1"
                     /translation="MNKELNITQEWDKVFPKSNQVNHRKVSFPNRFGITLVADLYEPK
                     EVSEKKLPAIAVSGPFGAVKEQSSGLYAQTMAERGFLTIAFDPSFTGESGGVPRYVAS
                     PDINTEDFQAAVDFLSVQKNVNPERIGIIGICGWGGMALNAAAIDTRVKATVVSTMYD
                     MTRVTANGYFDVMDENARYEMREELNAQRIIDFKQGDYARAGGVVDPLPEDAPQFVKD
                     YHAYYKTDRGYHVRSLNSNDGWNRTSGLSLMNAKLLHYIPEIRHAVLVIHGEKAHSRY
                     FGEDAFKELKGDNKELMIIPGASHTDLYDQTDIIPFEKMATFFEENLV"
     gene            3871..4305
                     /locus_tag="IXT53_002148"
     CDS             3871..4305
                     /locus_tag="IXT53_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ4905945.1"
                     /translation="MYSIGQVSEMFGLPVSTLRYYDKEGLFPDIQRVSGIRKFSENEL
                     EALRVIDCLKKSGLEIKDIKQFMEWSKEGSKTFEVRKELFEKQKEVVEKEIAKLERVL
                     AMLNYKSWYYEEAIKAGNEEAVLTMIPDDLPQHVKEAYVHSH"
     gene            complement(4430..5416)
                     /locus_tag="IXT53_002149"
     CDS             complement(4430..5416)
                     /locus_tag="IXT53_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HAQ4905946.1"
                     /translation="MEQGSLVKSAQDAYLQSIDLAVEAEKIGVDGAFFRVHHFAPQIG
                     SPFPLLAAIGAKTSKIEIGTGLIDMRYENPYYMAENAGLTDIITQGRVQLGVGRGSPE
                     QVLDGWKYFGYDYSEEEIKEVTRERTLEFLELLEGKPFEEPNPQPMFPQGSGKLRIEP
                     YSEGLRQRIWWGAGSQQTAIWAAQHGMNLQSSTLVNYESHEPFHIQQANQLRAYKEAW
                     KQAGHDFEPRTLVTRSIQPITTDLDRRLFGQDGSPQQDAVGYLAYHRNPTIFGKTFAG
                     EPDQLIKELAEDEAIKEADTVLVTIPNTLGVEYNMHLLETIVKDIAPELGWR"
     gene            5844..6266
                     /gene="arsC"
                     /locus_tag="IXT53_002150"
     CDS             5844..6266
                     /gene="arsC"
                     /locus_tag="IXT53_002150"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (thioredoxin)"
                     /protein_id="HAQ4905947.1"
                     /translation="MKKIYFLCTGNSCRSQIAEGYARKYLSPSMFEIRSAGIETHGLN
                     PRAIKIMAEDGIDISKQTSDLIDRDYFTQADLIITLCGDAKDKCPAVPNNHEHLHWDL
                     KDPAQAVGSEEEILTEFRKVREGIKRKVLDLNQRTSIV"
     gene            complement(6546..8468)
                     /locus_tag="IXT53_002151"
     CDS             complement(6546..8468)
                     /locus_tag="IXT53_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HAQ4905948.1"
                     /translation="MNQRQQSLIYQLSENNNYITTKKLAEHFHVSERTIHSDLNTIET
                     WLRDKHLPLMFERKKGTGILLNGLPHEREQLKRALNEETQTEMDKKQLRQLLIFHLLV
                     AKDGFSLEELSEKLYVGKRTIRKELTELQSFFEYHHLQLVSKTKLGTFLKGDEQEKRQ
                     LLVKTLRNMQKEDPQSPSLKEFFAKDTLKVIQHTLQDVFYENHLEQPNGLASVEIHIY
                     FMLERMKQYQKVKLSKDENEVVEHTQAQKLSSQILAKLATIYPIEFSPDEINYLALRI
                     ANLFTNSQAATRFQKESSALADHLIEQVEQFLGYSLKEDQLLKQNLRSHLSSTYFRLH
                     YGLQISNPLTKNVFSTYTQLFLVLQLILEDYFEKENFYVPQEETAYLTIHFQAAIERH
                     KHQKSRRYQTVLVSEYSKAMATFLEARLNRELPELTIMDLVEYKPELKRQDFPSVDFI
                     LATLPFKHISIPVIEISPMITETDLAYLTKYMLEHVPIKKKKTFDLASFTHPFLIFPQ
                     LERTDPADILNFMGNVLVEHHYVEPEFIDSVLERDSHSSTRVAPFVTIPHGNPLYVKH
                     SMISIATMKEPVLWHGEQIRIVLLLAIKKNDLKHAEFKKIFSVIHYLEKQPEQMAQLM
                     QSNHPLEILTLLSSYE"
     gene            complement(8555..10495)
                     /locus_tag="IXT53_002152"
     CDS             complement(8555..10495)
                     /locus_tag="IXT53_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIA"
                     /protein_id="HAQ4905949.1"
                     /translation="MKKIIAITSCPVGIAHTYMAAENLEKAGKAVGAEVKVETHGSIG
                     IENELTARDIEEAAGVIIAADTKIDKSRFGGKPLITVGVQEGIHHADELVRDILAGKA
                     PVYKSTEAIISSENKSESENLGKKIYKSLMNGVSYMVPFVVTGGLLIAISLTLGGTAT
                     PEGIKIPEGTIWATMNSIGSIAMGLMVPILSAFIAQSIADRPGLVPGFVGGMLAANGA
                     LYGSDANAGFLGGIITGFLAGFIALGIKKVRVPKALQSIMPIIVIPILGSLAVGFVFI
                     YVIGEPVALLFSSLTHFLAGMQGGSEIVLAMILGAMIAFDMGGPFNKVAFLFGSGLIA
                     EGNYSIMGPIAVAICIPPLALGLASLILKTKFTKTEREAGKASLAMGLFGITEGAIPF
                     ASTDPLRVIPSIVIGAMTGSVIAMLSGVTDHVAHGGPIVAVLGAVDHVAMFFVAVIAG
                     IAVTVLMLRILKPTLQQTAVAAANGVTVADLSETETSDVYTKTETFDSITDIMSDEAI
                     VLGETATTKDGVIEDLVQRLEKTAAITDKTRFKEVIYDREHESTTGIGMGIAIPHGKS
                     AYVLKPTVAFARSQSGVDWHSLDGKPAHMIFMIAVPENSQGDMHLKILQRLSRKLMDD
                     DFRQALMEAPDKTTVHQLLSEM"
     gene            complement(10783..11523)
                     /locus_tag="IXT53_002153"
     CDS             complement(10783..11523)
                     /locus_tag="IXT53_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KDGP aldolase family protein"
                     /protein_id="HAQ4905950.1"
                     /translation="MSVIPNYYKNRVCLNVLAHSVENAKACYEAAEGHVVLGVLSKNY
                     ASDEAAIEDMKRYQDATNNALSVGLGAGDPNQSQMVSRLSGVLQPQHVNQVFTGVGTS
                     RALLGQNDTVINGLVSPTGKIGYVNIATGPLSSQAPAAEVPIETAIQMLQDMGGTSIK
                     YFPMKGLAHREEFKAVAEACAKYEFYLEPTGGIDLSNFEEILKIAIDAGVKKVIPHVY
                     SSIIDKETGDTRIGDVKILLSMVKKIIH"
     gene            complement(11536..12627)
                     /locus_tag="IXT53_002154"
     CDS             complement(11536..12627)
                     /locus_tag="IXT53_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgaE family pyridoxal phosphate-dependent
                     ammonia lyase"
                     /protein_id="HAQ4905951.1"
                     /translation="MTISYGKFDLKEVINASGKMTILGVSKVSESVLAAQRFGGEHFF
                     EMSDLAIKTGAYLSQLLKVEDAQIVSSASAGIAQSVAALIGQGDMYHVYHPYTEKIAR
                     REIIIPKGHNVDYGTPVEVMVAQGGGKVIEAGYANMCTPEHVSMMITEKTAALLYIKS
                     HHAVQKSMLTVAEMVEVAKAHRLPLIVDAAAEEDLFKYSEMGVDLVIYSGAKAIEGPS
                     AGLVIGKKKYIQWIRLQSKGIGRAMKIGKDNILGFTQALEDYLKNGSESGDSMKQRLA
                     PFVNAINQLPDLSAKIVQDGAGRDIYRASVTINGDKSAKEVIQELKARNPAVYTREYQ
                     ANNGIIEFDIRSVSQNEMEKIIKRLREIM"
     gene            complement(12617..13723)
                     /locus_tag="IXT53_002155"
     CDS             complement(12617..13723)
                     /locus_tag="IXT53_002155"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase/deacetylase family
                     metallohydrolase"
                     /protein_id="HAQ4905952.1"
                     /translation="MFDTLIKNGRLVDGSKIEVAIEKGMIKAVAAEINQPAQEVVDLE
                     GKYYLSAGWIDDHVHCYEKMNLYYDYPDQIGVEKGVTTVIDAGTTGAENIREFYQLTK
                     SVKTNVYALMNISKWGIVKQDELADLTKIQEDLVSTALAELPEFIVGIKARMSKTVVG
                     ENGIKPLELAKKIQTENNDLPLMVHIGSAPPELEEVLSRMERGDVLTHCFNGKPNGIL
                     DPKTDQIKNFVWDAYNNGVVFDIGHGTDSFNFHVAETALKAGMKATSISTDIYIRNRT
                     NGPVFDLATTLEKLRVVGYSWEEIIEKVTAVPARNFHLKNKGQLAVNYDADLTIFEIV
                     ENEKELKDSNGFTRIAKEQILPIKTIIGGVIYDN"
     gene            complement(13742..14419)
                     /locus_tag="IXT53_002156"
     CDS             complement(13742..14419)
                     /locus_tag="IXT53_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4310 family protein"
                     /protein_id="HAQ4905953.1"
                     /translation="METTEEQAKVLEAKRQKSFWFADWSFPLIVGIMSAAVFAGTHMY
                     VMYGVGAFNEVSIVAMLKAGLDGGSYGAAAAFGASFLFARILEGSLVGILDLGGSILT
                     GIGIGVPAILLSMKIAAPIDNFALSLLTGLVLGLIVGGIIVLIRKFTINQSNSTFGAD
                     VMMGAGNSSGRFLGPLIILSACAASIPIGIGATLGALGFYAWKKPIAGGAILGAMILG
                     AIFPAVN"
     gene            complement(14430..15209)
                     /locus_tag="IXT53_002157"
     CDS             complement(14430..15209)
                     /locus_tag="IXT53_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4311 domain-containing protein"
                     /protein_id="HAQ4905954.1"
                     /translation="MDFVIILVKSVIIGGLLGFAASVGAARMFHAPNTQGLGAFRTLG
                     EMNACMGDPASHFSFGLGFFFNAWASSVGAGAFTQDVTHRIIPNWAAAALLAKNKDVT
                     QTMHDPKKMGIIGAVIGAVVVAFLNTTAAAIPEALQVTAVSVLVPAATILINTVMPVI
                     FWLAALDAGKRTGFWGTLFGGIAQLIMGNAVPGVVLGILIGKGVDESGWNRVTKSMMI
                     AIVLLFIFSGFFRGFDMEMIKSFQLQVPNWLDNLHNIFSVK"
     gene            complement(15238..15588)
                     /locus_tag="IXT53_002158"
     CDS             complement(15238..15588)
                     /locus_tag="IXT53_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4312 family protein"
                     /protein_id="HAQ4905955.1"
                     /translation="MSVQKTIQQKVIVSGKGETKQHAFASALAEIQNKIMEQSNVLLR
                     IEPVNVDILKAVENKYTERFLFFFFPRQRISYEVELSVTVAVTEIKLADISFSQQMTE
                     DPNGIAIPFVTKKI"
     gene            complement(15604..15960)
                     /locus_tag="IXT53_002159"
     CDS             complement(15604..15960)
                     /locus_tag="IXT53_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905956.1"
                     /translation="MIKVVIADRMGKGQNVAKGVEAAGGKAVVVPGMGADMRLGDVMQ
                     QEHADMGISFCGSGGAGALTAATKYGYPERHGMRSIEEGITAIKDGKTVLGFGFMDQE
                     ELGKRLTEEFIKKHGK"
BASE COUNT         4736 a         3344 c         2689 g         5191 t
ORIGIN      
        1 atttgtaata agttcgtata tgttttgcgg tgtttcttgt ttcttgtaat tttccacgaa
       61 tgtttcatcc atgaataaat tcataaacgc ttgaagtaat cctacttgtt cttttggttc
      121 tttgattcct aaaaaaaata gattttctac agtcatttcc tgattatccc ccatttgata
      181 aaaataaatt gggttcttca gctgcataac aaaaacaaat ggcttttcga catattcaca
      241 ttccgaatgt ggcagcgcga tatttaaata cttagtaatc aatccagtag gaaattctct
      301 ttctcttgca atcagtccat tcaaataatt ctcattgacg taccccattt tttctaattg
      361 ttgtgcagcg attaaaaaag cttcttcttt attttttact tctaccgcta attgaatcaa
      421 actaggaacg aacatctctt tatatttcat tccaacctcc tcctttttga ttaagtttgt
      481 taatttattg ttctactaag ttcatctttg ttttacaaga acattatatt tctgttagtt
      541 tgttttgtca acgctttcaa aagaaattta ttttgttaaa tttgatctac ttgaaaataa
      601 aatcgtttat ctatataatt aagaagaaca aagtcgaaca aaaattgact tttatgttaa
      661 aggagtaatt catatgctga aaaaagaaag acaagaacaa atcattacat tattagagaa
      721 agatttttat ttaactaatt cagaaatcgc taacaaatta ggcgtatcag aaatgaccat
      781 acgccgagat gtcactgaat tagcaaaaaa aaataaagta aaaaagctat atggtggcgt
      841 tgaacgaaag gaaccttata atcgagaact atcgactcaa gaaaagattg atacgaatgt
      901 agccgaaaaa aaatatattg gtcaagtcat gaacagtatg attcccgata attctactat
      961 ttatgtcggt gctggaacaa cgatcctata tgcacttcct ttcattcaaa agaaaaatct
     1021 atttgtaatt acaaacagtt tgattgcttt caactattta aagaatcata caagttatcg
     1081 gattttactt actggtggcg aattttctcc tattacagaa gagttcattg gggaagttgc
     1141 agaaagatct tttgaaagtt tgaatgtcga tatctctttc gctgcaacaa acggcatatt
     1201 tgataacaat gtgacaaccg ctcaatttat tgagggaaat attcagaacg cagcgctcaa
     1261 tgcagcaaaa atcaaatgca ttgttgcaga ttattcaaaa ttaaataaat cagacgtcta
     1321 tacttttaga aaattaaccg attttgatta tctaataacc gataatcatt tatcagaaca
     1381 ggatttcaat cattattcaa aatacttaac aatattaaag gagcccaaaa atgatactga
     1441 caatcacgat gaacccatcc attgattcca tttattttac aaaacacttt gaattaggta
     1501 cgatgacacg ctttgattct ccggtacgct atgtaggcgg caagggaatc aattgcggac
     1561 gagcttctgc tctactaggt tcatcagtta ttttaacagg gtttttgggc ggttattttg
     1621 gcagtatgat tcaagatatg ctgaaaaatg aaaatttgtt tgatttgcat tttttaccta
     1681 ttgcagaaga atcaagaggt gccgtcacga tcatgcatga tgatgaaacg cagacagagc
     1741 tagtggaagc tggtcctgaa atagcagata ctactatcaa aaaattaatg aatgatatca
     1801 aactaatcag taaaaataat tacctttcag tcattacaat aaatggttct gttaacaatc
     1861 cagatccctt tttgtatgct aaattattac aatatatcaa agaacaaata gatccttcca
     1921 cctatatatt aatggatata tctggacaac atctccgtga agtcttagca agtaagtata
     1981 aaccgatgtt catcaaacct aatatccatg agttttcaga tatcatccat caaaaaatta
     2041 ctcaaaaaag cgacataaaa caccatctaa aaacaaatcc actatttaaa gatatcgatg
     2101 ttatcatgat ttcttgcggc tcgtatggtg cagtagtcaa agtaaaagaa gaattttgga
     2161 atgttgagat ccctaagatt cctatcatta atacaacagg ctctggggat gccactgttg
     2221 gaggttttgc tcattcatta gaacagaatt atcctttaaa agaatgtgta aggtacgcaa
     2281 tggcttgcgg catgtcgaat gcacagcaat cagaagtcgg gaaagttatt ccagctgatg
     2341 taaaaaattt agcaaaaagc attaaaatca cagaaatgaa ataaatagta caaagtaaat
     2401 aattgtttat atgaaatgag acacaaagag actgtgacaa aaaacgtgtg taccgacccc
     2461 caaaagttag attttttggt ctaacttttg ggagtcgata cattgtcaca gtcctttttt
     2521 cgaataaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
     2581 gagaagctat tttttgaaat tgctccttgt gactcagagt taaacggcag gctcacgacc
     2641 tcttggtata aacggtgttc aaaagcttgc atctacgcta atgagtccaa ccaagtcaaa
     2701 aatgaggagc ttttttggct gtccttatta cttgatgcag ggcagttttt tcataatctc
     2761 ttttagatta aatccttttg gttacacaag gttctcttca aagaatgttg ccattttttc
     2821 aaatggaatg atgtctgtct ggtcgtatag atctgtatgg gatgctcctg gaatgatcat
     2881 gagttccttg ttgtcacctt ttagttcttt gaaagcgtcc tccccaaagt aacgtgaatg
     2941 agctttttcc ccgtgaatga ctaagactgc atgacggatt tctggaatat aatgcaatag
     3001 ttttgcattc ataagtgaca accctgatgt cctattccaa ccatcattgg agtttaaaga
     3061 acggacatga tatccgcggt ctgttttata atacgcatga tagtctttga caaattgagg
     3121 agcatcttca ggcaacggat caaccacacc tccagcacgt gcgtagtccc cttgtttgaa
     3181 atcaatgatg cgttgtgcat ttaattcttc acgcatttca tatcgggcat tttcatccat
     3241 aacatcgaag tagccatttg ctgttactcg agtcatgtca tacattgtgg aaacaactgt
     3301 tgctttgacc cgcgtatcga tagctgctgc attcaatgcc atacctcccc atccacaaat
     3361 accaatgatt ccgattctct cagggtttac atttttttgt acagataaaa agtctactgc
     3421 cgcttggaaa tcttccgtat tgatatcagg cgaagcaaca tatcgaggta ctcctccgct
     3481 ttctcctgta aacgatggat caaacgcaat cgttaaaaag cctctttcag ccatcgtctg
     3541 cgcatataga ccagacgatt gttccttcac tgcaccaaat ggcccgctca cagcgattgc
     3601 cggcaatttc ttttcactta cttcttttgg ttcatataaa tcagcgacta atgtgatacc
     3661 aaaacgattc gggaagctca cctttcgatg attgacttga ttgctttttg gaaatacttt
     3721 gtcccattct tgtgtgatgt ttaattcttt attcatattc tagactctcc tttgatcaaa
     3781 attgacattt gtttttgaat gtattattat gataaaactt aaacttgact ttaggtcaag
     3841 gtaaaaattt tattttagga ggattttttt atgtattcca ttggacaagt gtctgaaatg
     3901 tttggattgc ctgtttctac tttacgttat tacgacaaag agggattatt tcctgatatc
     3961 cagcgtgttt ctggtattag aaaattttca gagaatgagt tagaggcttt aagagtcatt
     4021 gactgtttga aaaaatctgg tttagaaata aaagacatta agcaattcat ggaatggagc
     4081 aaagaaggca gtaagacatt tgaggttcgt aaagaactat ttgaaaaaca aaaagaagta
     4141 gtagaaaaag aaatagcaaa attagaaaga gtactcgcta tgctaaacta caaaagctgg
     4201 tactatgaag aagcaataaa agccggcaat gaagaagccg tattgacaat gattcctgat
     4261 gatttacccc aacatgtaaa agaagcttat gtacattctc attgaacaaa cggataactc
     4321 actctccttc tctatgaact tcatgaaaac atgaataaac acctctaact gattgttaga
     4381 agtgtttatt ttttcactca agtatcctgc acttacaaaa atatcctttt taccgccatc
     4441 ctaattctgg agcgatatct tttacgattg tttcaagcaa gtgcatgtta tattctaccc
     4501 ccaaagtatt aggaatcgtg acaagtactg tgtctgcttc tttgattgct tcatcttcag
     4561 ctagctcctt gattaattga tcaggttcac cagcaaaagt ttttccaaaa atcgttgggt
     4621 ttcggtgata agcaagatag ccgacagcat cttgttgcgg actaccgtct tgtccaaata
     4681 agcgtcgatc caaatcagtg gtgattggct gaatcgagcg agtaactaat gttcttggtt
     4741 caaaatcatg acctgcttgc ttccacgctt ctttatacgc acgtaattgg tttgcttgtt
     4801 ggatatggaa tggctcgtgg gattcgtaat tgactaatgt agatgattgc agattcattc
     4861 catgttgtgc tgcccagatt gccgtttgct gcgaaccagc tccccaccag attctttgtc
     4921 gtaatccttc tgaataaggt tctatccgta attttcctga tccttgtggg aacattggtt
     4981 gaggatttgg ttcttcaaat ggttttcctt caagtaattc caagaattca agggtgcgtt
     5041 cccgtgttac ctctttgatt tcttcttcac tgtaatcata accaaagtat ttccagccat
     5101 ctagaacttg ttcaggtgaa ccacgcccca ctcctagttg cactcttcct tgcgtgatga
     5161 tatctgtcaa tccggcattt tctgccatat aataagggtt ttcatatcgc atatcaataa
     5221 gtcctgtacc gatttcaatc ttacttgtct ttgcgccgat tgccgctagc aaaggaaacg
     5281 gcgacccaat ctgaggggca aaatgatgta cgcggaaaaa cgctccgtcc actccgattt
     5341 tttctgcttc caccgctaaa tcaatcgatt gcaaataagc atcttgagct gatttcacaa
     5401 gcgatccttg ctccatccaa tgtccaaagc ttaaaaagcc gatttttttc aaggtaatga
     5461 cctcctttaa acctacaaaa atggaaggtt ttttattttt aatatgaact gattctacta
     5521 ctttttttct taaaagacga ataattgttc tcgcctcaca atcaagaaat gtatcggctt
     5581 actacacccc tactttcttc atcatctgcc gtgtcttaat ctcttttaac agtctgttac
     5641 atcgttgatt tttgactcaa aatgattgtt tctaacatgt atttatggta aaaaagacaa
     5701 gattagttct ttgtacacat cgatttatac gctgaacaaa aagaaatata taaactaaga
     5761 aacccattgt tttttcgctc catcacatag agtttacttt agataaacag tgacatttac
     5821 taatccataa cggaggaaat caaatgaaaa aaatctattt cttatgtacg ggcaattcat
     5881 gccgcagtca aatagcagaa ggctacgcgc gtaaatactt gtctccttca atgtttgaga
     5941 taagaagtgc ggggatcgag acacatggct tgaatccaag agctataaaa atcatggcag
     6001 aagacggcat cgatatttcc aaacagacgt ctgatttgat tgatagagat tactttactc
     6061 aggcagactt gatcattaca ctttgtggtg atgccaaaga caagtgccct gccgttccaa
     6121 acaatcacga acaccttcac tgggacctaa aagatcctgc acaagctgtc gggagcgaag
     6181 aagaaatatt aacggaattc agaaaagtca gagaaggaat caaaagaaaa gttttagact
     6241 taaatcaacg tacatcgatc gtataaagaa gtattcaaaa tgatttttca agcactaaga
     6301 aacaatgtta aaaaaatagc tttccatatt ttacaaaatt gtgccttatt gccgacaaat
     6361 tgtctcttga acaccgttta ccagtagtca agacatcgta agcaactata aaaaattata
     6421 agaaactcaa aataaaagga tgtgccacaa ctttggccac atcctctttt tttgtattta
     6481 gttttctact tccaaataaa tagatagaat tattataaat ataaaggcag ttttttaatc
     6541 gctacttatt cataactaga taacaacgtt aagatttcta gcggatgatt ggattgcatc
     6601 aactgagcca tttgttctgg ctgcttttct agatagtgaa taacagaaaa tatttttttg
     6661 aactctgcat gtttcaaatc atttttcttt attgctaata gcagtacgat acgtatctgt
     6721 tccccgtgcc ataggactgg ttctttcatt gtagcaatag aaatcatcga atgcttcaca
     6781 tacagcggat taccgtgagg gattgttaca aatggtgcta ctctagtact ggagtgactg
     6841 tctctttcca agacagaatc gatgaattcc ggctccacat aatgatgttc cactaagacg
     6901 tttcccataa agtttaaaat atcggctggg tctgtccgtt caagttgagg aaagatcaga
     6961 aatggatgag taaagcttgc tagatcaaat gtttttttct tcttgatagg cacatgctcc
     7021 agcatatatt ttgtcaaata ggctagatca gtttctgtga tcattggtga gatctcgatt
     7081 actggaatgc ttatatgttt aaatggtaac gtagccaaga taaaatcaac agagggaaaa
     7141 tcttgtctct ttaattctgg cttatattca actagatcca taatcgttaa ttctggaagt
     7201 tctctgttca gccgtgcttc gagaaaagta gccatagctt ttgaatattc actaactaaa
     7261 accgtttgat atcttctact tttctgatgt ttgtgtcgct cgatcgctgc ttggaaatgg
     7321 atcgttaaat aagctgtctc ctcttgtgga acataaaaat tttccttttc aaaataatct
     7381 tctaaaatta actgtaaaac taagaaaagt tgtgtgtacg tactaaacac atttttcgtc
     7441 aatggattag aaatctgcaa accataatgg agtcgaaaat aagttgaaga taaatgagac
     7501 cttaaatttt gttttagtag ctgatcttct tttaatgaat agcctagaaa ctgttccact
     7561 tgttcgatca aatgatcagc aagtgcgcta gattcctttt gaaaacgtgt ggctgcttgt
     7621 gaatttgtaa aaagattggc gatacgcaaa gccagataat tgatttcatc aggagaaaat
     7681 tcaattggat agatggtggc tagttttgct aatatttgac tacttaactt ttgtgcttgg
     7741 gtgtgttcca cgacttcgtt ttcgtcttta cttaatttca ctttctggta ctgtttcatt
     7801 cgctcgagca taaagtagat gtgaatctcc acactagcca gtccattcgg ctgttctaaa
     7861 tggttttcat aaaaaacatc ttgtaatgta tgttggataa cttttaatgt atctttagcg
     7921 aagaattctt tcaacgacgg actttgtgga tcttctttct gcatatttcg taatgtctta
     7981 acaagcaact gtcttttttc ttgttcatcg cctttaagaa aagttcctag ttttgttttg
     8041 gatactagct gtaaatggtg atactcaaaa aagctttgca actctgtcaa ttctttacga
     8101 atcgtacgtt ttccaacata tagtttttct gaaagttctt caagactaaa tccgtctttt
     8161 gctactagca gatggaaaat caataactga cgaagctgtt ttttatccat ttctgtttga
     8221 gtctcttcat taagcgctcg tttcagttgt tccctttcgt gaggcagacc attcaacaag
     8281 atacctgtac ctttttttct ttcaaacatt aaaggcagat gtttgtctct caaccaagtt
     8341 tcaatggtat tcaaatcgct atgaatcgtt ctttctgaca catggaaatg ctctgctagc
     8401 ttttttgttg ttatataatt attgttctca cttaattgat agatcaaaga ttgctgacgc
     8461 tgattcataa tgtgcagaaa ctcccttcta aataaaaaat cgatcatgat aacacatcta
     8521 ttatgtctca tcacggtcga tttttcatct tatgttacat ttcgcttaat aattggtgaa
     8581 ctgttgtttt atctggcgct tccatcagtg cttgacggaa gtcatcatcc atcaatttac
     8641 gtgatagacg ttgcagtatt ttcaagtgca tatcaccttg gctgttttca ggtacagcaa
     8701 tcataaagat catatgtgca ggttttccat ctaaagaatg ccaatctacc ccagattgac
     8761 ttctggcaaa tgccactgtt ggtttaagaa cataagctga ttttccatgt ggaatagcaa
     8821 tccccatacc gattcccgtt gtactttcat gttctctatc atagatgact tctttaaatc
     8881 tggttttatc tgtgatcgcc gctgtttttt ctaaacgttg gactaaatct tcgattactc
     8941 cgtcttttgt tgttgctgtt tcacctaaaa cgattgcttc atctgacata atatccgtga
     9001 tagaatcaaa agtttcagtt ttcgtataaa catcacttgt ttcagtctct gatagatctg
     9061 ctactgtcac accatttgct gcagcaaccg cagtttgttg taatgtcggt ttaaggattc
     9121 ttaacattag cacggttaca gcaataccag caatcactgc tacaaaaaac atggctacgt
     9181 gatccactgc tcctaaaacc gctacgatcg gaccgccatg agctacatga tctgtcactc
     9241 ctgaaagcat cgcgatcaca cttcctgtca ttgccccgat tacaatgctt ggaatcaccc
     9301 gtagtggatc tgttgaagca aacggaatag cgccttctgt aatcccaaac agtcccattg
     9361 ctaaggacgc cttaccagct tctcgttctg ttttcgtaaa tttggttttt aaaatcaatg
     9421 atgccagccc caatgctaat ggagggatac agatagctac agcaattggc cccataatgg
     9481 aataattgcc ttctgcaatc aagccagatc cgaacaaaaa ggctacttta ttgaacggcc
     9541 cccccatatc aaaagcaatc atcgctccta agatcatagc tagaacaatt tcacttcctc
     9601 cttgcatccc tgctaaaaag tgagtcaatg aactaaatag aagagctact ggttctccaa
     9661 taacatagat aaacacaaaa ccaactgcca atgatcctaa aattgggata acaataattg
     9721 gcatgatcga ttgtaaagct ttcggtactc gcactttctt gattcctaaa gcaatgaatc
     9781 ctgctaaaaa tccagtgatg atcccgccta agaatcccgc attcgcatca ctaccatata
     9841 atgctccgtt tgccgctaac atcccaccaa caaatccagg aacaagtcct ggacgatctg
     9901 caatactttg cgcaataaat gcagatagaa ttggaaccat caaccccata gcgatacttc
     9961 cgatactatt catcgttgcc caaatcgttc cttctggtat cttgatccct tcaggtgtag
    10021 ctgttcctcc taatgttaaa gaaatcgcga tcaataaccc tcctgtaact acgaaaggta
    10081 ccatgtacga aacgccattc atcaaactct tatagatttt tttacctaaa ttttctgatt
    10141 cacttttatt ttcggaagaa ataattgcct ccgtactttt atagactggc gcttttcctg
    10201 ctaaaatatc tctcaccaac tcatctgcat ggtggattcc ttcttgcaca cctacggtta
    10261 tcaaaggttt accgccgaat cgtgatttat ctattttagt atctgctgcg atgattacac
    10321 cagctgcttc ttcgatatct cttgcagtca attcattttc aataccgatg gaaccatgtg
    10381 tttcgacttt tacctcggcg ccaactgctt tccccgcttt ctctaagttt tctgctgcca
    10441 tgtatgtatg ggcaatacca actggacagg atgtgattgc aataattttt ttcattttcc
    10501 taccgccttt ctacatttat agtatataaa aacggtttct ttttattaag cacaaaatga
    10561 ttccgtaata actgaaattc ttggaataaa tagaaatttc gctaaaaaaa ttgatatacg
    10621 atttattatg ttgaataaaa taatattgga aaatcttaca tttctgtact tctgcttacg
    10681 tacatctaaa ttattatcta cattaacaat ttccgtaatt aattataaaa agcttattcc
    10741 atttaatttc attctggaat aagcttctta ttttctaatg agttagtgaa taattttttt
    10801 taccatgctt aatagtatct ttacgtctcc tatacgcgta tctcctgttt ctttatctat
    10861 aattgaacta taaacatgag gaatcacttt ttttacacct gcatcgattg caattttcag
    10921 aatttcttca aagtttgaaa gatcaattcc tcctgttggc tctaaataga attcatattt
    10981 tgcgcaagct tccgcaactg ctttaaattc ttccctatgt gctaagcctt tcatcgggaa
    11041 atacttgatt gaagtacctc ccatgtcttg cagcatttga atggcagttt caataggcac
    11101 ttcagctgct ggcgcttgtg aacttaacgg acctgtagca atattgacat agccgatctt
    11161 acctgttggt gaaaccaatc cattgatgac tgtatcattt tggcccaata aagcacgaga
    11221 agtccccacg ccagtaaata cttgattgac atgttgtggt tgtaaaaccc cagataaacg
    11281 cgaaaccatc tgactttggt ttggatctcc agcgcctaat ccaactgaca acgcattatt
    11341 ggttgcgtct tggtaccttt tcatatcttc gattgctgct tcatcgctag cataattttt
    11401 cgatagcaca cctagtacga catgcccttc cgctgcttca tagcatgctt ttgcattttc
    11461 aactgaatga gctaatacat ttaaacatac acgattttta taataatttg ggataactga
    11521 caatttaaaa gcctcctaca tgatttctct caatcttttt ataatttttt ccatttcgtt
    11581 ttggctgacg gaacgaatgt cgaactcaat gattccgtta tttgcttgat attctcgggt
    11641 gtagactgct ggatttcgtg cttttaattc ctgaatgact tctttcgcac ttttatcgcc
    11701 attaatcgtt acacttgctc gataaatatc tctgccggca ccatcttgaa ctattttcgc
    11761 tgaaaggtca ggaagttgat tgattgcgtt aacaaaaggc gctaatcgtt gcttcatgga
    11821 gtcaccggat tcgctcccat tttttaaata atcttctagt gcctgggtaa agcctaaaat
    11881 attatcttta ccgattttca ttgctcggcc aataccttta gactgtagcc gaatccattg
    11941 aatatacttc ttttttccta tgaccagtcc ggcactcgga ccttcgattg cttttgcccc
    12001 agagtaaatc actaagtcaa cgcccatttc gctatatttg aataaatctt cttccgcagc
    12061 tgcatcaacg atcaatggca aacgatgagc tttggcaact tctaccattt ctgccaccgt
    12121 cagcatactt ttctgaactg catgatgact tttaatatat agaagcgcag cagttttttc
    12181 cgtaatcatc atggaaacat gttccggtgt acacatattt gcatagccag cttctatgac
    12241 ttttcctcca ccttgagcaa ccattacttc aaccggtgtc ccataatcta cattatgtcc
    12301 cttaggtatg atgatttccc gtctagcgat tttttctgta tagggatgat agacatggta
    12361 catgtcgccc tgtccgatta aagcagcaac agattgagca atccctgctg aagcagaaga
    12421 aacgatttgc gcatcttcca ctttcagtaa ttgagaaaga taagcgccgg ttttgatggc
    12481 aagatcactc atctcaaaaa aatgctcgcc tccaaatcgt tgtgctgcta ggacactttc
    12541 agatacctta gaaaccccta ggatcgtcat ttttcccgat gcattaatga cttcttttaa
    12601 atcaaacttt ccatagctaa ttgtcatata tgacgcctcc aataattgtt ttaatcggta
    12661 aaatttgttc tttagctata cgtgtaaatc cgttggaatc tttcaattcc ttttcatttt
    12721 caacaatctc aaatatcgtc aaatctgcat cataattgac agctaactgg cccttattct
    12781 tcaaatgaaa attccgtgca ggaactgctg taactttttc aatgatttct tcccaagaat
    12841 agccgactac cctcaatttt tctaaagtag ttgctagatc aaaaactggg ccatttgtac
    12901 gattgcggat gtagatatcc gtactaatag aggtagcttt cattcctgct tttagtgcgg
    12961 tttctgccac atggaaatta aagctatccg tcccatgtcc aatatcaaag acgacaccat
    13021 tattgtaagc atcccagaca aagtttttaa tttgatctgt ctttggatct aaaataccgt
    13081 ttggttttcc attaaaacaa tgggtcaata catcgcctct ttccattctt gacaatactt
    13141 cttctaactc aggaggtgca gaaccgatat gaaccatcaa tggcagatca ttgttttctg
    13201 tctggatttt tttagctaat tctaatggct taatgccatt ttcaccaaca actgtcttac
    13261 tcattctcgc tttaatcccg acaataaatt ccggcagttc tgctaacgca gtacttacta
    13321 gatcttcttg aatctttgtt agatcagcaa gttcatcttg tttgacaatg ccccatttag
    13381 agatattcat tagcgcatac acatttgtct tgaccgattt cgtcaattga taaaattcac
    13441 ggatgttttc tgctcccgta gttccagcat caatgaccgt cgtcacacct ttttcgaccc
    13501 ctatctgatc aggatagtca taataaagat tcatcttttc atagcagtgg acatgatcat
    13561 cgatccaccc agcggataaa taatactttc cctccaagtc aactacttct tgcgccggct
    13621 gatttatttc tgctgcaact gctttaatca tgcctttttc tatagcgacc tctattttag
    13681 atccatcaac taaccttcca tttttaatca aagtatcaaa catttaatga ccctctttct
    13741 attaatttac tgccgggaag attgctccta aaatcattgc tcctaaaata gcacctccag
    13801 caattggttt tttccaagca tagaatccta gtgctcctaa tgtagcgcca atgccgattg
    13861 gaatagatgc tgcacacgcg cttaatataa ttaatggacc caaaaaacgt ccagatgaat
    13921 ttcctgcccc catcatgacg tcagcaccaa aagtggaatt tgattgattg atcgtaaatt
    13981 tacgaatcaa gacaatgatg ccaccaacaa tcaaacctag aaccaaacca gtcaataaag
    14041 agagtgcaaa attatcgatt ggtgccgcta ttttcatact taagagaatt gctggtaccc
    14101 caattccgat cccagttagg atcgaaccgc ctaagtccaa gatcccaact aaagagcctt
    14161 ctaaaattct ggcaaataag aaactagcac caaacgctgc cgctgcgcca taggatccgc
    14221 catccagccc ggcttttaac atcgccacga tcgaaacttc attgaatgca cccacaccat
    14281 acatcacata catgtgtgtt cctgcaaata cagcagcgga cataattcct acgattagcg
    14341 gaaaagacca gtctgcaaac caaaagctct tttgccgctt tgcttctaat acttttgctt
    14401 gttcttctgt tgtctccatg attctcctcc tatttcacac taaagatgtt gtgcaagtta
    14461 tcaagccaat taggcacttg aagctggaag ctcttgatca tttccatatc aaatccacgg
    14521 aaaaatccac tgaaaataaa taataaaaca atagcgatca tcatgctctt cgtgacgcga
    14581 ttccaaccgc tctcatcgac gcctttacca atcaagattc ctagaacaac accaggtacc
    14641 gcatttccca taatcaattg cgcgatacca ccaaaaagag tgccccaaaa accagttcgt
    14701 ttccctgcgt ctaaagctgc caaccaaaaa ataactggca ttactgtatt gatcagaatc
    14761 gtcgctgctg gtaccaaaac ggaaaccgcc gtcacttgta acgcctctgg aattgctgca
    14821 gctgttgtgt ttaagaaggc taccaccact gccccgataa ccgctccaat gatccccatt
    14881 tttttcggat catgcatggt ctgggtgaca tctttgtttt ttgctagaag tgctgccgct
    14941 gcccaattgg gaatgattcg atgcgtcaca tcttgtgtga atgcaccagc accaactgaa
    15001 gaagcccatg cattaaagaa aaagcctaaa ccaaatgaaa aatgagaagc tggatcaccc
    15061 atgcaggcat tcatttcgcc taatgttcta aaagctccta aaccttgtgt attaggtgca
    15121 tgaaacattc gagctgcccc gacagatgct gcaaaaccaa gtaatccccc gataatgacc
    15181 gatttgacta gaatgattac gaaatccacg ttattctttc ccccttatga ttcaaaatta
    15241 gattttttta gttacaaatg gaatggcaat cccatttgga tcttcagtca tttgctggct
    15301 gaacgatatg tctgccaatt ttatttctgt tacagccaca gtaacagaga gttcgacttc
    15361 atagctaata cgttgccgtg ggaagaagaa aaatagaaag cgttctgtgt atttgttttc
    15421 gaccgccttt aaaatatcca cattgactgg ttcgattcgc aataatacgt ttgattgttc
    15481 cattattttg ttttgaattt ctgctaacgc tgatgcaaaa gcatgttgct ttgtttcccc
    15541 tttaccggag acgataactt tttgctgaat tgtcttttga acgctcattt catgtccctc
    15601 tttctatttt ccatgctttt tgataaattc ttctgtcaga cgtttcccta attcctcttg
    15661 atccataaag ccgaaaccta gaacagtttt gccatccttg atagctgtga tcccttcttc
    15721 aatcgaacgc ataccatgtc gttctggata gccatatttt gttgctgctg ttaatgcacc
    15781 tgcaccacca ctaccacaaa aagagatacc catatctgca tgctcttgtt gcatcacatc
    15841 gcccaaacgc atatctgccc ccattcctgg tacgacaact gcttttccac cagctgcttc
    15901 tacccctttg gccacatttt gtcctttacc catacggtct gcaataacta ctttaatcat
//
LOCUS       DACGLW010000070        15924 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.073, whole genome shotgun sequence.
ACCESSION   DACGLW010000070 DACGLW010000000
VERSION     DACGLW010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15924
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.073"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            127..3654
                     /locus_tag="IXT53_002160"
     CDS             127..3654
                     /locus_tag="IXT53_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ4905957.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3651..7373
                     /gene="addA"
                     /locus_tag="IXT53_002161"
     CDS             3651..7373
                     /gene="addA"
                     /locus_tag="IXT53_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HAQ4905958.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7442..7783
                     /locus_tag="IXT53_002162"
     CDS             7442..7783
                     /locus_tag="IXT53_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ4905959.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8092..9135
                     /gene="pheS"
                     /locus_tag="IXT53_002163"
     CDS             8092..9135
                     /gene="pheS"
                     /locus_tag="IXT53_002163"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HAQ4905960.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9140..11560
                     /locus_tag="IXT53_002164"
     CDS             9140..11560
                     /locus_tag="IXT53_002164"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HAQ4905961.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11730..12740)
                     /gene="trpS"
                     /locus_tag="IXT53_002165"
     CDS             complement(11730..12740)
                     /gene="trpS"
                     /locus_tag="IXT53_002165"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HAQ4905962.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12994..13731
                     /locus_tag="IXT53_002166"
     CDS             12994..13731
                     /locus_tag="IXT53_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HAQ4905963.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13762..14559
                     /locus_tag="IXT53_002167"
     CDS             13762..14559
                     /locus_tag="IXT53_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HAQ4905964.1"
                     /translation="MRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEIIWGVKYD
                     TRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKNGNIDAI
                     IATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKGSTSAAN
                     IRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVGGTFTNE
                     PYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14572..15219
                     /locus_tag="IXT53_002168"
     CDS             14572..15219
                     /locus_tag="IXT53_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ4905965.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSTLAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15235..15891
                     /locus_tag="IXT53_002169"
     CDS             15235..15891
                     /locus_tag="IXT53_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HAQ4905966.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
BASE COUNT         5340 a         2745 c         3375 g         4464 t
ORIGIN      
        1 cgtatactta aaaaagtaga aatatcagaa ccttaaaagg atataatttg aagaatatag
       61 aacaaatgtt cagtattttt tgatataata aaggagcacc gtatcatgtg ttgaaggagg
      121 aaaagcatgt ctttacaatt tattcttgga aacgggaaaa aagatcaccg tcaagtcttg
      181 atcgacgaag catccgcttg gctcgaccag gatgcaagta accaagtatt tttccttgtt
      241 cccaactaca gcaaattcga acaggaacaa gaaattttag cggagatgcg aagaagaagc
      301 ggaaaaaaaa ctttcagcac aacaaacgtt caagtattta gtttttatcg tctggcttgg
      361 tatttgctgc aacagacaac tcttcttaca ggaaatgaat tatcagaatc aggatctgca
      421 atgatcttgc gacagatttt agaaaaaaat gaggaagaat taacaatctt tcgcggagaa
      481 atcaataaaa gagggtttat tcgtcaatta caagaactct acagtgaatt gcgtacagga
      541 aatattcaac cagaagacct gatgctttta tttggtgatt cacctaatgc agaagaccag
      601 caacttaaaa tgaaagactt gaagctgatt ttttcagctt atgacagcga attgactcaa
      661 cgtgcactaa caaatgaaga tgcactatct atactagcaa attatatgca gacacaagat
      721 ttcaacaacg tcaaatttat tgtcagtggt ttttctcgaa tcaatggtca agagtatcaa
      781 ttactgcagg caatgatgga aaaggggcaa ttggttgtcg atttattgct ggatcgtcct
      841 tatacagcgg atcttccaga agtacttacg ctctttcatg agactggaaa gctttatttc
      901 cgtctttttc aaagtgcaag agaacggaaa gtccctatat tagtggaccg atttgcacca
      961 gagcagactc gtccgacaga gcttcaactg ctgcaaacaa tgtgggaaga gacatctagc
     1021 caaaagaaag cggcagtaca caaaataggg ggacaagagc atctccattt gtgggaagct
     1081 gaaaatccaa ctgaagaagt ccgacaatta gctgttgaaa tcagacgact agtcagtgaa
     1141 gaaaactacc gttatcgtga tatccaagtg ctgacaaaaa atatgtcttt atatggaaat
     1201 atccttcggc cgatctttca agaaatggca attccttttt atatcgatga agaacaaatg
     1261 atggaaaatc atccgctgat cgaatggatc aacagtctgt tcgctcttga ccgctattct
     1321 tatcggctaa gtgatattat gcgttttttc aaaactgaat tatttctgtc gacggaagca
     1381 gaaaccgaac ttgatgaatg gaaacaagag cgagatgaat tcaggaaaaa actggacatc
     1441 actgaaaatg tagcacttgc ttacaattat caaggaactt attggactcg tgaaaaagac
     1501 tggcagtttg tggcgtatga ttttgaagca gaacagattg aagacgtaca gtatttggaa
     1561 gaacagtcaa atacgattag acgattattt cagaaagctg taccagtatt tttgaaaaaa
     1621 ataaagaaaa gcaaaacagg acaagaggcc gcaaatgttt tttatcaatt tttgatccaa
     1681 agtggcgtac agcaacaatt attgttttgg cgtaatcagg aagtagacag aggaaactta
     1741 gaagcggcaa gaaaccatga gcagacatgg ggagcgctga ttgatctgtt ggatgaatat
     1801 gtagtaattt atggtgaatc ggcttttgac tggtcactgt tccaagaaat cattacaagt
     1861 ggtctagaaa atctaagtta tggtaaaatc cctaccgcaa tcgaccaagt gcgaatcaat
     1921 cgtttggaat tagttcgacc aaatcaagct gccgtcactt ttgcattagg gttgaatgat
     1981 caagtgtttc cagaccgaaa agagacaaaa ggattgttgt ccagtgaaga acgagaagta
     2041 ttaaatcagg ccttgccaga tggacagttt ttattcgacc catcaaagga aagtatctct
     2101 tttgagcctt tccaagccta tcttgttttt ctttccggaa cagatcgatt atatcttagt
     2161 tatgcacaaa gctatgacac agatggttcg ctgaagatgt ctccttattt gcgtagaatc
     2221 acagaatatt tatctgttcc cgtcgagaga aaagagcatt tgacgatcga aagcgaccct
     2281 aattgttatg tagggactta cagaagcgga atcaatacag tcaataggat ttatcgatta
     2341 gctttagatg aaaaaaaacc tgtaccaaag cactggcaaa agctcaaaga attagtactg
     2401 aattcgtcat ggcgaaattt tgctttacag atttttgaaa gtcaggaaca tttaaatgtt
     2461 cctgtttcga tgccagaaaa aatggcggaa gagctttatg gaaaagagat ttatacttca
     2521 atttcacgta tggagaattt ttataattgt gaatacaaat acttcatgca atttggtttg
     2581 aaattgaaag aacgaactgt ttatggtcta actccagcag caactggtga tttttatcat
     2641 gaatcattgg atcgtttctt caagctactg ttttcaaacc aattatcctt agtgaatatg
     2701 tccgatgcgc agcgtagaga ctttactgaa aaagtgctgc aggaagtatt cggagaactt
     2761 cggtttgaga tattggatag ttctgcaaga atgaactata tccgctacca gttaggacag
     2821 acgattcaaa aagtgacatg ggctttgcaa aaacagagca aaaatacagg catgcatcct
     2881 cttcagacag agattctatt tggtcagatt gctggagcaa aaggtatcac aggtctggaa
     2941 ttaccgttga gtaatggtgg aaaactccat gttcgaggga aaatcgatcg aatcgatgta
     3001 gcttctgaac aagaagatac ttggctaagc gtcgttgatt acaaatctag cggacgttca
     3061 ttcgatgtca cagaagctta ttatggcatg gctatgcaat tattgacgta tctggacgta
     3121 gcattgatgg atgcggtacg tttgactgga aaggcggctg ttaaaccagc aggatcatat
     3181 tatttacatg ttcataatcc tgtattgacg gagacagatg atgtagagaa gaatacattg
     3241 aaaaaattta gttatgatgg cgtttttgtc gatgatcccg ctcttttaga agtactagat
     3301 cattcactgg aagcaaaaga acaatcactg atctatccag tgaaaaaaaa tgcgaaagat
     3361 gaataccaaa aaggtagttt cagtaaagac aaatttttca cggaagaaga actgcaacta
     3421 ttcatggcgc ataatcggga aaatatgcgg ttagctggag atgcgattac ttccggagaa
     3481 atcaaactaa atccagctta taaagataaa cagcggattg cctgcgaatt ttgtccattc
     3541 agaagcatct gtacgtttga tgtgatgctg aaagaaaata attaccaccg tctggagaaa
     3601 atagataagg aagaagcact gcgtagaata ttgaaaagga gtgaagaaga gtgaatcaaa
     3661 ttcctttaaa accgcaaaat gaacgattca cagatgatca gtggcaagcg atttttgatc
     3721 aaggagataa tctattagtc tctgcgtctg ccggatcagg taaaactaca gtgcttgttc
     3781 gaagagtgat tgaaaaacta aaaatgggtt ttgatatcga tgaattattg attgtgacat
     3841 ttactgaagc tgccgcaaga gaaatgaaag aaagaattca agaggcatta caagaatccg
     3901 tcaatagcga aagtgaccct gtacgtagac agcattttac taagcagtta gttttattgc
     3961 ccacagcgaa tatctcaacc ttacatgctt tttgcctgac agttattcga cgttattatt
     4021 acttaattga tattgatcca gtctttcgca tgttaacaga tgaaacagaa acgatcttga
     4081 tgaaggagga tgtgtgggac gagctgagag aagcacttta tgcagaaaat gatgaacgat
     4141 ttttccaatt aacaatgaat ttttctaatg atcgttcaga tgacggcttg acgaatctgg
     4201 tgttttctct ttatgagttt gcccgtgcca accctgaccc ccaaaaatgg ctagaacagt
     4261 taagcgataa ttatcgtttg cctgaggggc tagcgaaatc tcgattatat caaaaacaaa
     4321 tccgtccttt agttttggca gatatatacc aatgtgtaca gttatatgaa cagatgactc
     4381 agttagcaca aggtgaagga ttagaaaaaa tgcatgagca agtagctgga gaacaacagc
     4441 agatcaagaa tatctatgaa gcgttctcgc aagatcggtt agaagaagct tatgctggtt
     4501 tggaacaact aactttttca acttttaaga gcagtcggaa agcagaattg aaagaaattt
     4561 ccaatgaagt caagggcatg agggataaag caaaaaaact gattcagcag ataagtaaaa
     4621 gttattttcc tgtttcccca agccaaatgg aagaattaac ggacaaagca ctaccgttag
     4681 tcgaagaaat gacaaaagtc acgcaaagct tcatggacgg tttcagtatg cgtaaacggg
     4741 aaaaaggtgt gctggatttc aatgatctgg aacatttagc gttgcagatt ctcacagaaa
     4801 aaacgaaaga tgtatggctg ccaagtgaag cttcaaaaca ttatcgaaaa aaattcaaag
     4861 aagtaatggt agatgaatat caagacgtga accaattaca agaagccatt ttatactggc
     4921 taagagaacc cgatgataca aaaggaaata tgttcatggt tggggatgtc aaacaatcca
     4981 tctattcttt ccgtttagca gatcctagtt tatttatcgg gaaatacgag aatttctcaa
     5041 agaaagaagg cggacgtcga atcgttttgg ctgagaactt ccgctctaga aaagaagtcc
     5101 tttcttttac gaatcttatt tttgaacaac taatggatcc agcagtgggt caaatcaatt
     5161 atgacgaagc agccaaactg atccaaggat tttcagactt tccagagaat gaacagtttg
     5221 agccggaaat catgatctat gaaaaagaac aggaagaatc agagattgag attccgacgg
     5281 atgatatact ggaagacaaa acagaaggcg aactttttat gacaggacta aaaattcgtc
     5341 aattaattga ttcctccttt atgatttatg ataaaaaatc aaaaaaaagt cgtccgatag
     5401 agtacaaaga cattgttctc ttgacaccta caaagaagaa caacttaacg attttagaga
     5461 ttttcaaaac attggatatt ccattagaga tgaatgatgc tcaaaactat tttcaagcga
     5521 cagaaattcg cacgatgatc tcgctgctac aattaatcga taatccctat caagatattc
     5581 cgcttgcggc tgtattgcgc tcaccgatcg tgggtctgat tgaaccagaa ttagccagca
     5641 ttcgattagc taaccgtgca catacttact atgatgctgt gcttgcttat caagcatcaa
     5701 acgaagatga attagcagct aagctggaac attttggcaa gcaactagag cattggcgag
     5761 aacttgctcg ccgttcttcg atcactgact tgttatggga tatctattat gagacgggat
     5821 atctagagta tgtggtcggt ctgccagcag gggcacagag acaagccaat ctttatgcat
     5881 tggttgatcg tgccaaagct tacgagcaaa gttctttcag aggattatac cagttcgtgc
     5941 gtttcattga aaaaatgcaa gaaaaagata aagacttagc agaaccagtc atttcaatag
     6001 aagataatgc tgttcgcgtt atgacgatcc atgcaagtaa aggcttggaa ttcccagttg
     6061 tatttttgtt agatatgaca aaggagttca acttacaaga cttgcgcaat cggtatgctt
     6121 ttgaagaaaa acttggagca ggaattcgct atatggatcc tgaaacacga gtgttatatg
     6181 atacgttacc gtttcaagcc atcaaattgg caaagcaaaa caaattgttg tcggaagaaa
     6241 tgcgtaaatt gtatgtagga ttgactcggg cagaacaaaa gcttttcatt gtcggttcct
     6301 ataaaaataa agagcaaatg atccaaacgt ggtctgaagc agctgatcat gaggagttag
     6361 tttttgatcc tgcgctacgt ttgaagggca gaagcagctt gatgaattgg attggttacg
     6421 gattgatccg tcatcctgaa atgcagaaat acttagagga agaaatttcg acttctcttc
     6481 ttcaacattc aaatgcacag ttttctattt catggatgaa tcagcaatcg atcattgaac
     6541 aaagacagtt gcttgctgaa aaagaactgg ttaacttaga tcagcagatg aaagaagatg
     6601 aaactttatt agcagattcg ttacaaaaac gtctggcata tgaatatcct tatcaagctt
     6661 cttcacaaac cacaagttat caatctgtat ctgaaatcaa acgattattc gaagatccag
     6721 atgatacaca agaatcacgt ttgactctgg aaagcagtca gaacaaagca gcatcccgtc
     6781 aatttcgtta tacgcaagaa caactagcag aaccaaaatt tttgcaaaaa gatcggcaag
     6841 tgtcagcagc tgctgttgga actgctacgc atgctttgtt acaattactg ccattagaga
     6901 tgccaactac agagagtatc catcaaaaat tacaggaatt ggtaaggaaa cggttagtag
     6961 acgaaaaagt cgcgaaaaaa gtagatgttt cttctattat atggttcttt caaacagaac
     7021 ttggtcaaca gctgattgcc aacaaagaaa acgtaaaacg agaacaacct ttttccatgc
     7081 tgcttcctgc tgacgaagta tttcaagact atccaaatca agaagatgaa ctattaatcc
     7141 acggaatcgt cgatggatac ctagaagaaa aagatcatct caatatctat gattttaaaa
     7201 cagattttat tttacatccg gatgatccag cagaaataga tgcaatcgtc caaaaatatc
     7261 aaggacagct aagactttac caacaagcaa tgtcagaagc tttgaataag ccagtagaaa
     7321 atgttttcct aattttgctt cgagtgaaac aaattataaa cattaacaaa taattataat
     7381 tgagcaaact tgacttatgt tttgtaagga ttatgctata ctctgggaag aggtgagtat
     7441 gatggagcta acacagacag aattcgtttt atgccctaaa tttgaaaaaa cattctctat
     7501 tttaggaaaa aaatggaacg gattgattat cgatgtacta ctcgaaaacg gtccgcaacg
     7561 ctttgttgag cttgcggcaa aaatcccgat ggtcagtgat cgagtattag tcgaacgctt
     7621 gaaggaatta gaaaaagaag gaatagtcac tcgaaacttt gcctgtagcg aaaagagccg
     7681 ttcagaatat ctcttgactg aaaaaggagc agacttgcaa aaagcgatgc gtcaaataca
     7741 agtttggagc gaaaaatgga tcacagagga agaatgcggt tgattttgta aaaaaaatct
     7801 tgtaaactat agtccatacg aataagtaaa gaacgatgac gaaaaagagt agtttggcta
     7861 aaccagttca gggagatctg tcgcagactg aaagcagatc ctgtgtttga aaaatgaagt
     7921 tcactttccg agttcgtctg ataaaatcag accggcaatt gtcgttaacg aagtaagagt
     7981 gcggtgtaag tcatttatcc gcaaattagg atggtaacgc gaagacctcg ttccttaaca
     8041 ggaatgaggt ctttttgttt tgtatttgga atttaaagga ggaacaagca catgtctcta
     8101 caagcacaat tagagagctt gaaagaagca acgctcgtca aaatcacagc ggcacaagat
     8161 ttaaatgaat taaatcaaat acgtgttgaa acgttaggaa aaaaaggacc gatcacagaa
     8221 gtattacgtg ggatgaaaga tctaagtcca gaagagcgac caaaagtcgg aagtttcgca
     8281 aatgagatcc gagatctttt gacagaagag atcgagcgaa aaaaacaagt attagaagaa
     8341 gaagcgttaa atgcagcttt ggcaaaagaa acaatagacg tgacactacc tggtagaaaa
     8401 gtaaaacatg gtacacgtca tatcgtgaca caaatcatgg aagaaattga agatattttt
     8461 gtgggtatgg gctatcagat cgtagaagga tatgaagtag aatctgatca ttacaatttc
     8521 gaacggatga atctgccaaa agatcatcca gcaagggaca tgcaagatac tttctatatt
     8581 tcagacgaga tattgattcg gacacatact tcaccagtcc aagcacggac aatggaaaaa
     8641 catgatttct ctaagggtgc tctacgaatg atctcgccag ggaaagtctt ccgcagagat
     8701 acagatgatg cgacccacag tcatcagttc catcaaattg aagggctagt tgttgataaa
     8761 aacatcacaa tgggcgacct taaaggaaca ttagaagtcg taatgaaaaa aatgtttggg
     8821 gaagatcgtg aaattcgttt gcgtccaagt tatttcccat ttacggaacc atcagtagaa
     8881 gtagatgtca gctgtttcaa atgtggtggt gccggttgta acgtatgtaa atacactgga
     8941 tggatcgaaa ttttaggagc tggcatggta cacccgaatg tgttgaagat gtcaggaatc
     9001 gatccagaag aatattcagg ctttgctttc ggccttggac cagaccgcgt agctatgttg
     9061 cggtatggag tgaatgatat ccgtaatttc tatcaaaatg acttacgttt cttaagtcag
     9121 ttcaaggggg agtaagaaaa tgttagtttc atataaatgg ctcaatcgtt atgtagattt
     9181 atcgaatgta acaccaaaag aattagcgga taagatgtct gtgacaggaa tcgaagtaga
     9241 gggagtaacg gttccagaag aaggcttgaa aaaaatcgtt gtcggtgaag tcaaggaatg
     9301 cgtaccacat ccagattctg atcacctttc tatttgtcaa gtcgatattg gcgaagacga
     9361 actgtctcaa atcgtttgtg gagcgcctaa tgtgaaggtt ggaatcaaag tgattgttgc
     9421 acttcctaac tcacgtatcg ctggtaatgt aaaaatcaaa aaaggaaaaa tgcgtggcca
     9481 agtatcaaat ggcatgattt gctcattaca agaaattggc tattcagata gtgttatacc
     9541 gaaagaatac gctgaaggta tctattatat gccacaagaa gctgtaaatg gcgatccagt
     9601 ttttccttat ttggatatgg atgattcgat tattgaatta tctatcacac cgaatcgtgc
     9661 ggatgcattg tcaatgcgtg gcgtagctta tgaagttggg gcaatttatc gtcagactcc
     9721 tacattcgat gacgaaaagc tggtagaatc ctcacgtcct gctagcgata aaattcaagt
     9781 ggaagttgaa gataaagaag cagttcctgc ttatcaaatc agaatcattg aaggagtaag
     9841 aatccagcca agtcctcaat ggctgcaaaa tcttttgatg aacgaaggca ttcgtccaat
     9901 caacaatgta gtagatgtga cgaactatat tttgctttta ttcggacaac cactacatgc
     9961 atttgactac gacaaattga aatcaaaaga gatctttgtc cgccgtgcaa aagaaaatga
    10021 aacattagta acactagatg gagaatctcg ggaattgaca actgacaatc tcgtcattac
    10081 taacggcaaa gaaccagttg ctttagctgg tgtcatggga gggttagact cagagatcac
    10141 gaatgaaaca acaacagttg cactagaatc tgctttattt gatccaattt cgattcgtcg
    10201 tacagcaaaa caatttaatt tgcgtagtga atcctctgca cgttttgaaa aaggaatcaa
    10261 ccatgcgact gttggagaag ccagtgaggt tgctgcagca atgatcgttt ctcttgcggg
    10321 tggggaagtt ctacaaggcg cggtcaaagg ctcagagatc aaagcagaag acgtatcagt
    10381 gaagatttca cttgaacgca tcaatcaata tttaggaaca tctcttgtcg ttaaagaagt
    10441 aaatgagatt tttgaagcac ttggatttag ctatgaagaa aaggagtatg tttattccgt
    10501 agtagtacca ccaagacgct gggatatcca aatcgaagca gatattatcg aagaagttgc
    10561 tcgtatttat ggatacgacc agttgccatc tacacttcca agtggagaaa cggttgctgg
    10621 aagcttgtca aaagaacaag aaactactcg taaaattcgt acaatctttg aaggaagcgg
    10681 attgagcgaa gcaatcagtt atgcattgac aacagaagaa aaatcaagac agtttgttat
    10741 aaatgaaagt aatattacac gtttggactg gccaatgagc gaagaacgtt ctgtattacg
    10801 aatgaatttg atcagcggac tattagatga tatcagttat aacactgccc gaaaaaatac
    10861 agagattgct ttatatgaaa ttggtcgggt gttcttccaa gaaaatgatc cacgtacaca
    10921 tctgccgcaa gaagtaaaac atgcagcaat cgctatttct ggtatttggg aagaaaaaga
    10981 ttggcagacg aaagctcaaa atgtagactt tttcaccatc aagggtcttt tagaaaactt
    11041 atttgaacaa ctaggcatca ctgaagatgt ccattatcaa acagtgacag aaatgaagga
    11101 aatgcatcct ggaagaactg ctgcgattta ccttggcgaa aaatttattg gttttgtcgg
    11161 tcaagtgcat ccaacgatta caaaaagtta tgatattcca gaaacatatg ttgctgagtt
    11221 tgatttatct gctatagtgg aagctgctca aaaagggatc gttttcgaag cagtgacaaa
    11281 attcccagca gtcagccggg acatcgcttt gcttgtaaaa gaaactgtga caaatcagga
    11341 attaacagaa gttatccgtt ctgctgccgg aagattttta acagatatcc aattatttga
    11401 tgtctatcaa ggagaaaaca ttgaaaaagg acataaatcc atggcgtata gcctgacatt
    11461 cgtcaaccca gaagcaacat tgacagatga agaaatcaat aaagcaatgg aaaaagtcga
    11521 aaaagcgttg gtagaaaacc tagaggcaag tattcgctaa gaaggaaaat atagtaagta
    11581 acgatcaagt agcttttgaa gaccatacat ttgaaaaaaa gatgtgacac aacttcgtca
    11641 catctctttt tttgatttta aaaaaggaat agcacagaga cgagataaaa aaacaggcct
    11701 ttacagcctg cttttttaga gtaaatgaat taatggaagt aattgattcc cattgctgat
    11761 tttacttcag aaagtgtttg tgcagctact ttagcagctt cttcactacc tttacgtaag
    11821 atttccataa cagctgaagg atctttttcc aattcttctc ggcgcgcacg gatcggtgca
    11881 aattcagctt ccaatacatc aatgaggtat cgtttgatct ttacatcacc aagtccgccg
    11941 cggcggtaat gagctttcat ttcttcaatt gcttctttat ctgttccgaa tacatccaag
    12001 taggtgaaga ccatattccc ttctacttgt ccaggatctt gtacatggat gtggtttgga
    12061 tcagtataca tactcatgac ttttttctgt aagacatcgg caggatcgga gatataaata
    12121 ccgtttccta acgatttact cattttgcca tttccatcga ttccaggtaa tctacccatt
    12181 ccttttggtg ggaatacgcc ttttggttcc accaagactt caccatatgt atgattgaag
    12241 ctttgtacga tttcctgtgt ctgctctagc attggttttt gatcctcacc aacaggtaca
    12301 agattcgctt tgaatgcagt gatatctgct gcttgagaaa caggatagat aaagaatcct
    12361 gtaggcacgc tttcaccgaa ttttttttgt tcgatttctg ttttgacagt cggattgcgc
    12421 cgaacgcgtc ctacgctgac taagttcaaa taatacatgg ttaattcagc taattccgga
    12481 atttgtgatt ggataaataa agtagatttt gcaggatcta atcccacggc taaatagtct
    12541 aaagcaactt gcaagacatt tgatgaaacc ttctcaggat ttttagcatt atctgtcagt
    12601 gcttgcatgt cagcaatcat gacaaacagt tggttgtctt catctgcctg catagccact
    12661 cgggttttta gagaaccgac gtaatgccct aaatgcagct tcccagttgg tcgatctccc
    12721 gttaaaataa tatttttcaa aaaagacacc tctttacgat ttgtaagtct gagcacttaa
    12781 tatcttaccg tttttcctga aaagaaacaa cagttcctaa ggaaaaaaca aggaaagagg
    12841 ataaaataac taatagatta gaaatggaag aaatatttat gggagaataa acgttctaag
    12901 aaactttaca aattactaaa ctatcagata taatgtattt tattatacat ctttaaaaga
    12961 aatcttacta aataataatg ggagtgtaat tctatggcaa tgattaaatt tgatcacgtg
    13021 gataaatatt atggcaagtt ccacgcatta aaaaatatca atctcgaatt cgagaaagga
    13081 gaagttgtcg ttgtcattgg tccttctggt tcaggaaaga gcacgatgct tcgctgtatc
    13141 aatggattag agacgatctc ttcaggtaag ctgctgatta atgatactga cttgcatgat
    13201 aaaaagacaa aactgacaga agtccgaaaa aacatcggca tggttttcca acatttcaat
    13261 ttgtacccaa ataaaactgt tctggaaaac atcaaattag caccaatcaa agtgttgaag
    13321 caagaccagg caactgcagt caaaaacgcg gagaaatttt tgaaaactgt caatatgtta
    13381 gataagaaag attcttatcc ttccatgtta tccgggggac aacagcaacg ggtggcaatc
    13441 gccagaggtt tagcgatgaa tcctgagatg ctgctgtttg atgaacctac ttctgctttg
    13501 gatccggaaa tgattggtga tgtattagac gttatgaaaa aattggctca tgacggtatg
    13561 tcaatgattg tcgtgacaca tgaaatgggc tttgccaaag aagtggcgga tcgtgttatc
    13621 ttcatggctg atggagaagt attggaagac agcagaaatg tgagagactt tttcgaaaat
    13681 ccaaatgaag tccgagcaca gcaatttatc agtaaagtta tcaatcattg ataggaggaa
    13741 aaagatgcgc aggttaaaaa aatacgcctg attttccttt taggcattgc ttttcttgca
    13801 ctagccggat gtaaaggaaa tgggttggca aatgaagata ttttagagcg aagcaaatct
    13861 accaatgaaa tcatctgggg tgtcaagtat gatactcgat tatttggaat gatggatatt
    13921 gagagcagaa ctgtacaagg atttgatgta gatattgcaa aagcaatcac taaaaagatc
    13981 cttggtgaca atggaaaaac agaatttgtt gaagtcactt ctaaaactcg gattccatta
    14041 ttgaaaaacg gaaatatcga tgcaattatt gcaacgatga cgatcacaga tgaacgaaaa
    14101 aaacaggttg acttttcaga tgtctatttc gatgctggcc aagctttgtt ggtaaagaaa
    14161 ggaagccaaa tcaaaagtgt agatgatttg aatgcttcta caacggttct agcagtcaaa
    14221 ggttctacat cggctgcgaa tatccgacag catgcaccag atgcaaaaat tttagaatta
    14281 gaaaactatg cagaagcatt tactgcttta caatctggtc agggagacgc gatgactacg
    14341 gataatgcga ttttgttagg gattgcagat gaaaatccag agtatgagtt agttggtgga
    14401 acgtttacta atgagcctta cgggattgcg atcaacaaag ggcaagaaaa cttcttgaaa
    14461 gctgttaacc aagcgctaga ggaaatgcat gcagatggca catacgataa aatctatcaa
    14521 aaatggtttc caaatgaaac tgaaggaaaa gtagagtaag ggggagaggc aatgagcgaa
    14581 ttgatacaaa cttatggacc aatctttgtc gacgggttca agatcacatt gttttcgagt
    14641 gtcttagcac ttgtgtttag tttgattatc ggaacgttaa tggcaatctt ccaattatca
    14701 aagaataaag tagtgagcac cttagcaaag gcatatgtag aattttttag aaacattcca
    14761 ttattaatca tcgtgatgtt tttttatgtt gtcgtaccga tttattggtt taatctagat
    14821 ggattccaag caggtacgat tggattaacg atctatacat ctgcttttat tgctgaaaca
    14881 gtacgttcag ggattcaaac cgtaccaaaa ggtcaaacag aagcaggact atcttctggt
    14941 tttacctatt cagaaatcat gcgttatatt gttttaccgc aagcattcaa aatcgttatc
    15001 ccgccattag gcaaccaatt tatcaacctc gtaaaaaact catctatttt agcaatcgta
    15061 gctggtttag atttgatgta tcaaggagac ttgatcgcta gtgaaacatt caataccttc
    15121 gatacgtata tcatcgttgg gttattttac ttagttatca cgctaccgtt atcttactta
    15181 atggtttatc ttgagaaaaa atgggcggtt cgttcataga aggaggggaa atacatggat
    15241 tttagcggcg ctttttcatg gatgaatatc cgttttttat tggaaggatt aaaggttact
    15301 gttgaagttt cattgttttc gatcattttt agtttcttga tcggtggtct gacaggtgtg
    15361 attcgttttg cgaatatccc tttcttttcc aaaatccttg gtgtagtgat tgatattata
    15421 cggaatcttc cattattatt gatcattttc ttcacttact ttgccttacc tcaaatcggc
    15481 attcagatga acatcttctg gtccgctgtc gcagcattaa cgatttttga gtcagctatg
    15541 ttgtcggaaa ttttccgagc tggactcaat gctgttccaa aaggacagat ggaagcagga
    15601 ttatcaacgg gactgactta tgtagaatcg atgcgaacga tcgtgttacc gcaagcattt
    15661 aaatcaatga tcccggcaat tgtgagccaa ttgatttcac tgatcaaaga tacttcatta
    15721 gcagtaatca tttctttacc tgaactttct catcaggcac gtattattta tggacagaac
    15781 accaattatg tgcttccgat gttcatcatc atgacactta tgtacttcgt ggtatgttac
    15841 gcgctttctc tgctttccgg ttacttggaa aagagacggt acagttatta atagatgatt
    15901 cttttagcgg ttgtggcaaa ctaa
//
LOCUS       DACGLW010000071        15825 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.074, whole genome shotgun sequence.
ACCESSION   DACGLW010000071 DACGLW010000000
VERSION     DACGLW010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15825
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.074"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            complement(266..466)
                     /locus_tag="IXT53_002170"
     CDS             complement(266..466)
                     /locus_tag="IXT53_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ4905967.1"
                     /translation="MKINYTVDLVSFSLVINFCLFSLRLFIEGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(771..2003)
                     /locus_tag="IXT53_002171"
     CDS             complement(771..2003)
                     /locus_tag="IXT53_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAQ4905968.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSAAGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVIDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            2260..2829
                     /locus_tag="IXT53_002172"
     CDS             2260..2829
                     /locus_tag="IXT53_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ4905969.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(3007..3447)
                     /locus_tag="IXT53_002173"
     CDS             complement(3007..3447)
                     /locus_tag="IXT53_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HAQ4905970.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLSGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            3605..4369
                     /gene="map"
                     /locus_tag="IXT53_002174"
     CDS             3605..4369
                     /gene="map"
                     /locus_tag="IXT53_002174"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HAQ4905971.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            4401..5324
                     /locus_tag="IXT53_002175"
     CDS             4401..5324
                     /locus_tag="IXT53_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HAQ4905972.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            5400..6539
                     /locus_tag="IXT53_002176"
     CDS             5400..6539
                     /locus_tag="IXT53_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HAQ4905973.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            6532..7332
                     /locus_tag="IXT53_002177"
     CDS             6532..7332
                     /locus_tag="IXT53_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ4905974.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            7332..8159
                     /locus_tag="IXT53_002178"
     CDS             7332..8159
                     /locus_tag="IXT53_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ4905975.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            8137..8871
                     /locus_tag="IXT53_002179"
     CDS             8137..8871
                     /locus_tag="IXT53_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HAQ4905976.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            8971..9837
                     /gene="rfbA"
                     /locus_tag="IXT53_002180"
     CDS             8971..9837
                     /gene="rfbA"
                     /locus_tag="IXT53_002180"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HAQ4905977.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            9851..10423
                     /gene="rfbC"
                     /locus_tag="IXT53_002181"
     CDS             9851..10423
                     /gene="rfbC"
                     /locus_tag="IXT53_002181"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HAQ4905978.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            10445..11473
                     /gene="rfbB"
                     /locus_tag="IXT53_002182"
     CDS             10445..11473
                     /gene="rfbB"
                     /locus_tag="IXT53_002182"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HAQ4905979.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            11571..12422
                     /gene="rfbD"
                     /locus_tag="IXT53_002183"
     CDS             11571..12422
                     /gene="rfbD"
                     /locus_tag="IXT53_002183"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HAQ4905980.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            12456..14489
                     /locus_tag="IXT53_002184"
     CDS             12456..14489
                     /locus_tag="IXT53_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905981.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPAKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKIDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKEIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            14533..15813
                     /locus_tag="IXT53_002185"
     CDS             14533..15813
                     /locus_tag="IXT53_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HAQ4905982.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIATLLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
BASE COUNT         4862 a         2732 c         3280 g         4951 t
ORIGIN      
        1 tttccttcca tcagcataaa agttcacctc aaaaagagta tacgaacgaa tgttcttttt
       61 gtgaaggcgg aatttaatat actgaataat agaaaacaag tacttgtgcc aaattatgtg
      121 ccaaaaaaat tcgaattcaa caaaatgtaa acaaatagaa aaccttctat attaagattt
      181 cctgcaataa caaacacaac agaagacgtg taatagttag tcaggaacgt acaaataacc
      241 cctgtatcct ttgcggtaca gggggttatt ttgtataagt agacattgta gtttctaaac
      301 tgctatgtcc taaacgtttc gatacgctaa gtatatttac tccttgataa agtaaaatag
      361 atgcatgcgt atgtcttagt ccctcaataa acagtcgaag agaaaatagg caaaaattga
      421 ttactaaaga aaaacttact aggtcgacgg tgtagttgat tttcatactt caacagattc
      481 gaattaaacg atttcgattg gcttatacca gtcctatccg acgaaagata tatcgttctt
      541 aactctagcg gtataacttg aagctttacg taaagccaat gggaatgaac tggagatgac
      601 tttttattat ttttagtaat agctgatggg ttaatactta caactttcct tttcaaagta
      661 ccatataaaa aaacggtggg gttgtttagc gaaataaagg atctaagcca atagcttaga
      721 tcctttattc tatgctttat attatttttt gcatgtgtgc gaaagtatac tcaagcccaa
      781 tcgccatttc ggaaaatcgg tactgtagag ccatcttcac ggattccgtc gatatccatc
      841 ttgtcagatc ctaccatgaa atctacgtgg gtttgacttc tattcaatcc agcttctgct
      901 aattcttcat cggacatttc tgtaccgcct ttcacactga aggcataagc agaacctagt
      961 gctaagtgat tcgatgcgtt ttcatcaaat aatgtattga aaaagatgat gccagattga
     1021 gaaataggtg aagggtcagg tacaagtgcg acttctccta aacgacgcgc accttcatct
     1081 gtatcaagca gttttgctaa gacatcttct ccttgttcgg cagaaaaatc aatgactttt
     1141 ccatctttaa aagtaaattt catacccgag atgatggtgc cagcataact cagtggcttt
     1201 gtacttgaaa tgtaaccatc tactcgatga ctgtcaggtg ctgtgaacac ttcttccgtc
     1261 ggcatatttg ccatgaattt ttctccacga gcattatagc ttccagcgcc ttcccaaaga
     1321 tggtttttag gcaatccgat aatgatatct gtaccgggag cagtatagtg cagggcagaa
     1381 aattgttctc gattcaattc ttctgctttt tttgctaatt tttcatcatg cttcttccat
     1441 gctaagacag ggtcttcttc atagacacga gtcgttttga aaatctgatc ccataatgcc
     1501 tcaacttgtt tttcttctgg cagttctggg aagacttttg ctgcccattg tttaccagcc
     1561 gcagccacga cagtccaact gactttattt gcttgagttg ccttgcgtaa attcattagt
     1621 gctttgccag ccgctgattg ataagacgct actcgatggc tatctacacc agcaaatgca
     1681 tcgggatcgg cagaaacaac actgatcctg cttgctcctt tttcaagcca ttcatccgct
     1741 tgatcgattt tagattgtgg tacattctca atacgatccg ttgcagcatg taaaagaaat
     1801 tctctttgga tctggtcgtc agtccattga acgataacct cagcggctcc tagtttataa
     1861 gcttcttgtg tgatcaatcg agcaaggggt gcctgttcca cgctgatctg taaaacaact
     1921 gtgtgtcctt tttctgtggc tacgccagtt tctgcaatta agcgtgcata tttttttaga
     1981 agttcattaa aatcagataa cataataaaa attcctcctc accatttttt ctggtaacta
     2041 ttagcatgat accatttaat tatgattcaa acaatgaggc ctttaaataa aaaaaagagg
     2101 ttattttttt aaggttaagt ttcatgatat atgttactac ttgctatttc agcttgtgaa
     2161 gaaaatcaaa aactaatttt gcatatcaaa aaaaacatgt tacaatgaaa ttaatcaatg
     2221 gtggaagcgt tataaaaaaa ttaaggagat taaaaaaata tggcaagaaa aaagacgatt
     2281 actagagaac agattttggc agctgcttat gaagtagctg ccaccgatgg atttacacga
     2341 tttactgcga gaaacatcgc gaacaaaatg aattgttcca cgcaaccgat ttatcttgag
     2401 tttaaaaata tggatgactt gagacaagct ctttttgagc agatctatga tcatttacaa
     2461 tatgaagtgt tcactgttga gcatacaggg aatacgatta tcgatcttgc ttttaattat
     2521 atccattttg caagaagaga gaagaagttg tatcgttcac tgtacttaga agaatatggt
     2581 ggaggaaaag agatgcagaa tttctcttat acttactttg tgaatacagt gaaacgcgat
     2641 ccagcttatg tgaatctttc agaagagcag atcgaatctt tgtataatgg gacatggatc
     2701 atagctactg ggatcgcagc tttgatgtct tcaagcatca tccatccaac tgatgaacag
     2761 attgaaaaga tgatacaaga cagtatagat gctatcttga aattcaaaga accaatagaa
     2821 atcgattgaa tatactcaaa agttccacaa atcagtagat aacaagagat gtgtcaaaat
     2881 aaggaaaagt tcgtttttct tacagcgtat gcatttttaa ctcccgaaga actcttccat
     2941 gaatgcaatg aataaaagag ccgtacagat tgaataaatc tggatggctc ttttattgca
     3001 agtatattaa atttttgcga ctagttcttt agcgaaagct tctaaatttt cgatatcttc
     3061 ttcttcagca ttcagatcca ctttaacatt ttcggctcct tttgatgcgc ctgtttttgc
     3121 aaaagcagca tcgaaatcat ccaccgattt gcagaattca tcgtaaaaag tatcaccaga
     3181 tccgcagaca ccgtaaattt ttcccgatag atccaattct tgcaagtctt cataaaagtc
     3241 tacgatctca tcaggcagat cgccatcatc gtatgtataa gtagctacta cgcaaatatc
     3301 tgcctcctcg aattcatctg catctacttg ggtacattcg ttgatttcga cttcgatatc
     3361 cagattttca aatgcttctg caacaatatc ggcgatttct tcagtgtttc ctgtcatgct
     3421 agcatatacg atttttgcta aagccatgat gtgcctcctc gtttataatt atttaacaat
     3481 tatgaattat accaaaacat cattgaaaag aaaataaaac ctaaagacac ctggggaatg
     3541 tcatttggct aaatgtatgc cgctatgtta aaatgagagg gatttgtaaa aaggagacga
     3601 aaatatgatt actttaaaat cagcacgtga aattgaaatg atggcagaat ctggagcttt
     3661 attagcagat gtacataaaa atctgcgtga ttttataaaa ccaggaatca caagttggga
     3721 tattgaggta tttgtcagaa actatatcga gagtcatggc ggaattgctg cgcaaattgg
     3781 ctttgaaggg tatgaatatg cgacttgtat cagtataaat gatgaaattt gtcatggatt
     3841 ccctcgtaaa aagcctttga aaaacggtga tttggtaaaa gtcgatatgt gtatcgattt
     3901 gaaaggcggc atttctgatt cttgttgggc ctatgtagtt ggagaatcta cacctgagat
     3961 cgaccgattg atggaagtaa cgaagaaagc tttgtacatc ggtattgagc aagcacaagt
     4021 agggaatcgg attggtgaca tcggtcatgc tatccaaact tatgtagaag gcgaaaatct
     4081 agcgatcgtt cgtgatttta ttggtcatgg cgttggtcct acgattcatg aagaaccagc
     4141 agttcctcat tacggagaag ctggaaaagg gttgcggttg aaagaaggca tggttatcac
     4201 gatcgaacct atggttaata ccggaacttg gaaaatgaaa atggatccta acggctggac
     4261 tgcgtataca agagacggcg gactttcttg ccaatatgaa catacattgg ctattactaa
     4321 agatggtcca agaattttga catcacaagg agaagaaggc acttattgat ctatccagtc
     4381 aataggtacg ggagaggtac atgaaagttt ggaacaaaat aaaacaaaat gaaaatctga
     4441 tgcgctttat tgagacgact caaaagcgca tcactgattc agaaatgagt acgacctctg
     4501 tcgtcgttgc ttattattta ctcttgtctt tatttccatt gattattgct tttggtaata
     4561 ttttaccgtt tctccacatc gatcaagaga ctgtactgac ctatttacga gaagtgattc
     4621 ctgagaccgt ttaccagttt atcggtcctg caatcaaaga ccttttgaca caaagttcag
     4681 gaggcttgtt atccgtttct gcattagcgg ctttatggtc tgcaagtcaa agtatcaatg
     4741 ctttgcaaat tgcgatgaat aaagcgtatg gcgtagaaaa tagaaaaaat tttttgatcg
     4801 tccgtttctt ctcactaatt gtcattgtct tgttcatgat tgctattagt ggggtaacta
     4861 tcgttcttgg gttgggacag ctgattttgg atgctattca gccaatcgta caaatacccg
     4921 tgaactttat cgatcagttc caaactttga agtggccaat cacattggta gctttatttg
     4981 tcatcatgtt cttgatttat ttaattgtcc ctaatgctca gttgaagttg aagacgatca
     5041 ttccgggcac gatcttcgct actgtcggct ggatgctgtt atcacaagtg tttagtatct
     5101 atgctaggta ttttgcaacg aggatcagcg gttatcagat cataggaagt tttattgtac
     5161 tcatgctttg gcttaatttt gctgctacga tcatcatttt gggcggaatc atcaatgcag
     5221 ttgttcaaga atacgtgacg ggtcatcaaa tcaaaaaacg tcgagatcca agccgaaaat
     5281 ggttcaataa attgaaaact aaattttctc ataaaaaaaa gtagaaacaa gagttcttat
     5341 tgtaaattta tatcagtaat ttataataat tatgactctt aattaaagta ggtgcccata
     5401 tgcttgtttc ttttagtatg gttataagat tttttctgac aattttatta tcactgatat
     5461 taacacctgt attcaaaatc atttcagtac aaacaggtat ggtcgacaaa ccaaatgaaa
     5521 gaagaataaa taaagttcca atgccttcag cgggcggact gcctatcttt atttcatttg
     5581 ttatatcgac tttattctta ttccgtaaaa ttatccctca atcgtatatt gtaccgatct
     5641 tgattgcttc tttagtaatc atagtcacag gagtgctcga tgataaatat gagctgacac
     5701 ctaaacaaaa aagtataggg atcttgattg cttctcttat tatttatttt gtagcagata
     5761 tacgtattga ttccttcacg cttcctttta ttggatatat ccaactaggc tggcttagtt
     5821 ttcccgtaac gattttttgg atcttcggta tcacgaatgc ggtcaatctg attgacggtt
     5881 tggatgggct ggcggcaggg atttcattga tcggtttaat tacgatagga atcattggat
     5941 attttttcct ccatgcttct acagtgtata ttccagttgt tattttctgt cttgtagcaa
     6001 gtattatcgg tttttttcct tacaattttt atccggcaaa gatttattta ggcgatacag
     6061 gagctctatt cctagggttt atgatggccg tcctttcact acaagggctg aagaacgtga
     6121 cttttgtttc atcgatttct ttgctgatcg tcatgggagt gccggtaaca gacacgtttt
     6181 ttgctatcat taggagaaaa gcaaaccgtg tttctttttc tacagcagat aaaaaacatc
     6241 ttcatcaccg actaatcagt ttaggcttta ctcataaagg tgctgtcctg acgatttatg
     6301 cgatggcttt gatgttttct ttcacagcta tggtcatgaa ttatactgga agaatcggaa
     6361 cgatcatatt gattattgcc atgttgtttg ctgcaatttt attatttgaa ttgattggtc
     6421 tgatcaatga gaaacatcag tttattttaa aaacattgcg atttttaggg aataaagagt
     6481 accgtacgca agtaaaacag aaatatttga agaaatggaa ccgtcatata gatgaataac
     6541 aaattaaacc agccgatcct tattagtatt gtgacggcaa acagtgataa aatttttcgg
     6601 acactagata attttatttc aactgtaaaa gatgaaacag cagtaaaaat ccgaattttt
     6661 gacaacaatt cacttcccga gtacaaaaaa agactaacag agtataggaa atatccctac
     6721 atcgatatct attttcatga tgaaaattcc ggatttggtt atggacataa tcacaatctg
     6781 atagctagcg attctcgcta tggcattatt tgtaacccag atattttagt ggatgaagag
     6841 acagttacag cattggtctt gttattgaaa gaacatccgg aatgtgcgat gttagcacct
     6901 aagattctta atgaagatgg aacaactcag cacctcatcc gtgagcagct ggctgttttt
     6961 gattacgttt tgcgctttgt gcctttcaaa tgggtcaaaa aaaagtttga caaacgactg
     7021 gctgtgtttg aatgccgagg attgcctact gatgctaata gttatgtacg gatgatctca
     7081 ggtagtttta tggtagctga tattgataaa tttaaagaga taaaggggtt tgatgagcgt
     7141 ttcttcatgt actttgaaga caacgacttg tgtttgagtt ttgaaaaagc aggttacaag
     7201 ttattgtaca caccctttta tgcagttatc catttttacg ggaagggtgc gcatcgaaat
     7261 gcaaaactgt ttcgcgtgtt tttgacttct atgatgaaat ttttcaataa gtgggggtgg
     7321 cgttttttct gatgaaacca gaaatattag tagtcgttgt gctttatcag caactattca
     7381 gtcaatctcc agcttatgga ccattaagca aagcacttag cgaaaagcaa attcagctta
     7441 ttatttacga taatagtcct caaaggcagc aaaatgagct gtttgaaaaa gaaaatatcg
     7501 tttatcatca tgatccgcaa aatcctggtt tagcggtggc ttataatttt gcgttgtctc
     7561 aagcagatga aggaacacgt tgtctcgtca ctctggatca ggatacgagg ctagtcgacg
     7621 attattttga actattgcgt aaagtaacct ttacagatga atgtgttgct gcagttccga
     7681 tgattttttc tgggagccga caagtttctc ccctatattc agatcgttat atcaatcgta
     7741 cagctcaagc agtagaagtt gaaggcccaa cagctgaacg aatcatggca atcaattcag
     7801 gtgttgcctg gtctgtcaaa tttctaaaaa agattggcgg gtttaatcct gctttttctt
     7861 tagatttttt ggatcattgg ctgttttgga aagtggatca gttggaaaaa actgtggaga
     7921 tccttcctgt acgaatggaa catgatcttt cggttttaga ctatgaaaaa gtttcaagta
     7981 atcgataccg ttctatttta caagcagaaa atcgatttta tcatgaatat gatcaaaggc
     8041 atttatccag tcaccgacga caattattgt taagaacagc taaacagttc ttgaccgtca
     8101 aaaaccggca tatctggcgg atgactttgc gctcatatgt taagaattgg aaggtgtgaa
     8161 ttcgtgcgtt cggtttgtat agctaccttt aatggaggca gatatttaag agagcagtta
     8221 gatagtattt tatctcagct ttcagcagaa gatgaagtag tcatttctga tgatgggtca
     8281 gtagatgata catgggatat attaactgaa tacgcagaaa aagataagcg aatccagctt
     8341 ttttcggggc ctcgtcaagg attgatcgct aattttggtt atgcgatcca gcagacgaaa
     8401 ggagatctaa tctttttggc agaccaagat gatgtttggt tggagacaaa agtgaaaaag
     8461 atcactgact attttgagca gaatccggat cagctagtag tagtttctga tttaatcatt
     8521 gtggacagcg agctgcaaga gatccatgat tcgtattttt cgtttcgaaa ttccaaagaa
     8581 ggatggctta ataatgtgat aagaagtcac tatattggtg ctggtatggc tttccggtct
     8641 tctttaaaac ccgatatttt gccgatcccg tcagaagtgc cgatgcatga tatgtggatt
     8701 ggtttgctgg gaggaaagaa gactgggttc ctaagagaac cgttaactct gtaccgaaga
     8761 catgatttaa acgcgagtga gattgaaaca cattctaaat tacggcaaaa aatttcttgg
     8821 cgtgttcatc tgattacggc gttgatcaaa cgaaaatggt ttaaacgttg ataaaagatg
     8881 tttaaatcct taatatttcc atgacaaata taacaattga tgagataata acaaagtgaa
     8941 atttgaaatc aaaaaggaga gcagatatcc atgaagggaa tcattcttgc tgggggcagc
     9001 ggaacacgtc tgtacccttt aacaaaggca acatcgaaac aattgatgcc gatttatgac
     9061 aaaccaatga tttattatcc aatgtctact ctgatgttgg cgggaatcaa agaaattctg
     9121 attatttcaa cgccacaaga tacaccacgt ttcaaggaat tatttggaaa cggaaatgat
     9181 ttaggactgc agatcgaata tgcagtacaa gaaagtccag atggattggc acaagcgttt
     9241 atcattggag aagaatttat tggagatgat agtgtctgct tggtattagg ggacaatatt
     9301 tactatggtg gcggtctgtc taaaatgttg caacgagcag cttcaaaaga tacaggcgcg
     9361 acagtatttg gctatcatgt caatgatcca gaacgctttg gtgtagtaga atttgatgag
     9421 aatatgcaag ctttgtcgat tgaagaaaag cctgctcagc caaaatcaaa ttacgctgta
     9481 actggccttt atttttatga caatgaggta atttctatcg ctaaaggtat caaaccttca
     9541 gaacgtggag aactagagat tacggatgtc aataaagcat atttggataa aggaaaacta
     9601 tccgtagaac ttatgggacg cggatttgct tggcttgata caggaacaca tgaatcattg
     9661 cttgaagcat ctacctttat cgaaacaatt gaaaaacgtc agaacttaaa agtggcttgt
     9721 ttggaagaaa ttgcttaccg tatgggctac atcgataagg aacaattgct ggcccttgca
     9781 cagccattga aaaaaaatca atacggtcaa tatttattga atttggccgc tgaataagga
     9841 gtccttacag atgaaagtaa cagatacaaa attaacagat gtcaaaatcg tagaaatgga
     9901 cgtctttggc gatcatcgcg gtttcttcac agaaagttat tcaaaagcaa aatttgcaga
     9961 acatggattg aaatatgact ttgttcaaga caaccattca ctatcagctg aagctggtgt
    10021 ccttcggggg cttcatttcc aaaaaggaga agctgcacag acaaaattga ttcgggtagt
    10081 cactggagca gttttagatg tagtagtgga tatccgcaaa ggtagcccga cttatggtca
    10141 atgggacgga tttatcttat ctgaacataa tcatcgacag ttgcttgtac ctaaaggata
    10201 tgcccatgga ttcgttactt tgacaccaaa tgtgaacttc ctttataagt gtgataatta
    10261 ctataatgca gaagcagatg ggggcatcgc atttgatgat ccagatttag cgattgattg
    10321 gccaattgat ccatcaagag cgattatgtc ggaaaaagat caccgtcatc cgacattaaa
    10381 agaatttgaa gcagaaaatc catttgttta cggagaaatc taagaattaa ggagaaattt
    10441 tcttatgaaa aacatcattg ttaccggagg tgccggattc atcggctcta atttcgttca
    10501 ttacgtggta aacaatcatc cagaagtgca tgtcactgtt ttggataaac tgacttatgc
    10561 aggaaacaaa gagaacttgg ctggacttcc tagcgatcgc gtagaattgg tcgtaggaga
    10621 tgttgcagat gcagaattag tggaccgtct agtaaaagaa gcggacgcag ttgttcatta
    10681 cgcggcagaa tcccataacg ataattcatt aaaagatcct ttcccatttg tacaaacgaa
    10741 tatcattggt acgtatacac ttttagaagc ttgccgcaag tatgatgtac gctatcatca
    10801 cgtatctact gacgaggttt acggcgatct tccattacgt gaagacctgc caggacatgg
    10861 ggaaggcgaa ggagaaaaat tcactgcgga aacaccatat aatccttcaa gtccatattc
    10921 ttctacaaaa gctggttcag atctgttagt aaaagcatgg gtgcgttctt ttggcttacg
    10981 tgcaaccatt tcaaactgtt caaacaacta tggtccttat cagcatattg aaaaatttat
    11041 tcctcgccaa gtaacgaata tcttaagcgg tattcgtcca aaattatatg gcgaaggaaa
    11101 gaatgttcgt gactggatcc atacgaatga tcattcttca gcagtgtggc tgattttgac
    11161 gaaaggcaga attggcgaaa cctatctgat cggtgcagac ggtgaagaag ataataagac
    11221 cgtgatggaa ttgatcttag aaatgatggg tcaaccaaaa gatgcgtacg accatgtaaa
    11281 cgaccgtgca ggacacgact tgcgttatgc aatcgattcg acaaaattac gggaagagtt
    11341 aggatggaag cctgagttta cgaacttccg tgatgggttg gctgacacga tcaaatggta
    11401 cgaagaacat gaagactggt ggaaaaaaga aaaagaggcc gttgaagcag catatgccaa
    11461 aaatggacaa taatacagag aaaatgtcac tgtattaatg gaaaaacaca tgtgaggcag
    11521 gcgctcgcct gtctcttttt taaaataaaa atcaaggagc ggataatcat atgtttttat
    11581 taactggagg aaatgggcaa ctaggtacag aattgcgcca tttattagat gaaaaaaatc
    11641 tgcaatatgt ttcaacagat gcaaaagact tggatatcac agatgccgag aaaacaatgg
    11701 cttacatcac agaattaaaa cctgatgtta ttttccattg tgcagcttac acagcagtag
    11761 ataaagcaga agaagaagca aaagaattag atgagaagat caatgttgat ggtacaagaa
    11821 atgtagcact tgcagcaaaa gctgtgaatg ctcaattcgt ctatatcagt acagactatg
    11881 ttttcgatgg aaaaaacaaa aatgtagaat acgaagtaga cgaccaaacg aatccactaa
    11941 atgaatatgg tcgtacgaaa ttattaggcg aaaaagcagt agaagagatt ttagaagact
    12001 attatatcat acgaacttct tgggttttcg ggatctatgg acacaatttc gtcttcacga
    12061 tgcaacgcct agcggaaacc cgcgacaaac tgactgtagt aaatgaccag tttgggcgac
    12121 caacttggac acgtactttg gcagaattta tggtatatgt catcgaagaa aaagcaccat
    12181 ttggtattta tcatttgtcg aacgaaaata gctgttcttg gtatgaattc gcaaaagaaa
    12241 tattgaaaga tacagatgta gaagttgcac ctgttacgtc agaagaattt ccgcaaaaag
    12301 caacacgtcc gcagtattca gtcatgagcc tgaaaaagac agaagagtta ggatttgtca
    12361 taccaacttg gcaagaagca cttgcgcaaa tgctggaagc tgccaaaaaa atgaataagt
    12421 aatttccttt tatcaaatag aatttggagc atgatatgga aaaacaaaca agaagtgcac
    12481 gacgaaaaga aaagccaaaa gcgaaccagg cagattcgaa tttggtgctt gacctgtttc
    12541 taaaactccg ctatcttatt ggcgttttag tcatcgtggt catcgttact tttaatttaa
    12601 acggaagttc acttggtgtt tgggataaat atgtgtcgca aagagacgat gggaaaaaaa
    12661 ctgatgtgat ttttggacag aatcgggaag tgcggtcaga cgaatggctg gtgcagacac
    12721 ctttttacct ctcacaagct gaagaggact ttccgttggt gaacgaggat tattcattaa
    12781 acggacaaaa catgatcatt gcgtataatt ctcctgcaaa ggatattacg gttatcggaa
    12841 aaccattcaa ctggggattc tttttcctcg gaagagatcg aggactttcc ttttattggg
    12901 cgatgaaatt agtcgtcatg gtcttgcttg gattcgaagt actgatgata ttaacgaaga
    12961 gaaaaaaagc tctatcgcta atcggtgcat ttgcgctgac attttctcca gcagtccagt
    13021 ggtggttcat gcaacatgtg ggcgatttga ttttctttac tttgggactg atgactgctt
    13081 tttatcatta tttctacaat catgataaaa aatggctacg aacgttgatg atgcttttgg
    13141 tggttatttt cggtattggg tttattttag tgatttaccc agcccatcaa gtgatgctgg
    13201 cgtacttgct gattttttat tttgttggcc tgcttattta ctatagcaag aaagtttcat
    13261 gggatctgtt ggatgtagtg ttgattggag gggcagtttt atttattggc ggagtcatgg
    13321 ttcatttctg gctaacgtca aaagacgcat tgttagcttc tttgaatacc ttatatccag
    13381 gaaaacgggt aagtactgga ggaaagtgga cgattggcga atttttttct ttccttacaa
    13441 attggaaaat tccttttgaa gatatcaact attcgaataa ctctgaagtg gccctgttct
    13501 atcacttttt tccaactgta ttcctagcaa gtccatttgt gttatttggc aaaaaagata
    13561 gcgaacaaaa gttactggga cgtattttga tgattttttg tctgttttct atcttttgga
    13621 ttaccgttgg gttgccaaaa tggctagcag aaatcactct gctgtcttat gttcctccat
    13681 taagagccat ccttactttt agttttgcgg cttgtttatt atcgatctgg tttatcgctt
    13741 atatttggag agaagatgtt attcctattt ggtataaatt cattttgttg gcagtaaatg
    13801 catggtctta tttctatgcg attacgcatt cgaaaataga taattatttt tcggattttg
    13861 aaaccatcgc agttgctgtg ttgggaacag tgattttagc atttgcatta tttggctgga
    13921 gacgcttgtt ttacctcgct tttgccgcct tagttatcgt atctggcttt ttcgtcaatc
    13981 cagtcgtaga aggtacagga gcgatttttg aaaaaacact ggcaaaagaa atccaaaaaa
    14041 tcgatcaaca agatcctggt aaagtttggt tgtctgaaga tgaattatat aacttcaccc
    14101 caacattggg agtgaaatcc ttcaatacag tacgctttta tccagatata gctgcatgga
    14161 aggaaatcga tccgaaagaa gaatacgaga agtattataa tcggtatgct catacaagag
    14221 cttttatcgc taaggaagat acaaagttca ttttagatcg tccagatatg ttttcggtca
    14281 cgttgaattt tgaagacgca gaaaaattag ggatcgacta tgtgatcagt aaacgaccat
    14341 taactgatta caaccaatta tatgaagcgc agttcacgca gctatatgga ccggataaag
    14401 acggttatat gatttttaaa gtagaatatc cggattatcc agatgtacca caaacacaaa
    14461 ctcaggaaaa tcagctagtc gtacagtaat aggctagctg agaataatcc tgaaatatta
    14521 gtagaaagga gagtggagat aattcaagca atcgtacaaa aatttgtcaa caaaatgagt
    14581 gtcggtttct tgcttttgtt gctgacgcta gtcttttttg tttggacagc agggttaggt
    14641 tttccagcaa ctttgtttat atataacaat agttcattac ttatcgcttt tagtgtggct
    14701 attttgctca ttttaaatgt ttataaagca aaaggcgtcg attttcttta tctaggtgtt
    14761 gcgcttgccc ttttcatttt cttttcttac tttcgggaat taagaaatga gtctttattt
    14821 ggagatttac tgtttccttt gattatttta ttttgtttag taattaaatg gatcgagtgg
    14881 acaaaatggg atcgcggtat ttatctgatt gtctttacgg tcttcctcgg aatcacgtta
    14941 tatcgtgtat ttacagagat ccaagtacca gaaggacaaa gcatctgggc tccaggcaat
    15001 tcgctttctg atatttggat caatacgaat acaatcggaa gttctttgat gactcttgct
    15061 ttattgatca gtggatttgc cagttctttt gaacgatggt atgtacgaat catcggtgca
    15121 gtagcagttg ttgcagcttt tctcgggatt tgggtctgcc aatctagagg tgcattagtc
    15181 gcgttgattg tttttgcgat attggatctt ttacctaaat cgttgatgcg tgtcattcgt
    15241 gcgccattta ttgcttacac ggttacgatt cttttagctt tgccgatttc ttatctggcg
    15301 gctgtctctg aaaaagtcaa tttgtttacc ggtcgagaag atatttggca taaattctat
    15361 cagacgctag gagaaaaatc tgaacaagtt ttactaggaa tgaaaacatt tatcttccaa
    15421 cgaggaaacc aatttttagg aaaccacaat agttataatt ctattttgaa tatctatgga
    15481 ttgataggat ttgggattgc tactttgctg cttatccttt ttattggtcg cttaacgttg
    15541 aaagcagatt tatccaacgg gcagatgact tttatctggg ccttttttgc ggtgatgatg
    15601 cagtcattta tggaggatac attgacttca tacccatggg ttccgattat ttatatcctg
    15661 ttggctatgg ccgttcatcg atatgatgaa ccagaagttg cggaggaatt ctatgttatg
    15721 gaagaggcat atgacgatat gacttatgat gatacatctt atgaggaaca accagatagc
    15781 gctcattctt ccaggatcca acgctatcat tgataggcac aaaat
//
LOCUS       DACGLW010000072        14924 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.075, whole genome shotgun sequence.
ACCESSION   DACGLW010000072 DACGLW010000000
VERSION     DACGLW010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14924
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.075"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            complement(<1..398)
                     /gene="zapA"
                     /locus_tag="IXT53_002186"
     CDS             complement(<1..398)
                     /gene="zapA"
                     /locus_tag="IXT53_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HAQ4905983.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILN"
     gene            600..1517
                     /locus_tag="IXT53_002187"
     CDS             600..1517
                     /locus_tag="IXT53_002187"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HAQ4905984.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            complement(1773..2684)
                     /locus_tag="IXT53_002188"
     CDS             complement(1773..2684)
                     /locus_tag="IXT53_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ4905985.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(3087..3665)
                     /locus_tag="IXT53_002189"
     CDS             complement(3087..3665)
                     /locus_tag="IXT53_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ4905986.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            complement(3685..4353)
                     /locus_tag="IXT53_002190"
     CDS             complement(3685..4353)
                     /locus_tag="IXT53_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4905987.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            complement(4368..5438)
                     /locus_tag="IXT53_002191"
     CDS             complement(4368..5438)
                     /locus_tag="IXT53_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ4905988.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            complement(5609..6973)
                     /locus_tag="IXT53_002192"
     CDS             complement(5609..6973)
                     /locus_tag="IXT53_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ4905989.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            complement(6970..8529)
                     /locus_tag="IXT53_002193"
     CDS             complement(6970..8529)
                     /locus_tag="IXT53_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ4905990.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            complement(8585..11065)
                     /locus_tag="IXT53_002194"
     CDS             complement(8585..11065)
                     /locus_tag="IXT53_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905991.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            complement(11049..11384)
                     /locus_tag="IXT53_002195"
     CDS             complement(11049..11384)
                     /locus_tag="IXT53_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4905992.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            complement(11398..12432)
                     /locus_tag="IXT53_002196"
     CDS             complement(11398..12432)
                     /locus_tag="IXT53_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HAQ4905993.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            complement(12522..13202)
                     /locus_tag="IXT53_002197"
     CDS             complement(12522..13202)
                     /locus_tag="IXT53_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HAQ4905994.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            complement(13486..14589)
                     /locus_tag="IXT53_002198"
     CDS             complement(13486..14589)
                     /locus_tag="IXT53_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HAQ4905995.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
BASE COUNT         4329 a         3063 c         2573 g         4959 t
ORIGIN      
        1 tcgtttaaaa tctgttgtgc ttccacatga tttttaatgg tatgatctgc ttgaccattt
       61 tctttcaata cttcccgagc ttcgttctcg attgcttcaa tgcgtttcac acgattttca
      121 agctctgtaa atttgatcat ttttttcttt aattctgctg tttcttcttc cagctccaag
      181 attctttctt gtttcttcaa ttgatccgaa agcgcattga cagccatcaa gattgctgct
      241 tgttcattat ccatttgtgg tgataattgt ttcaattctg ctaactgttc attgatcagc
      301 ttcgttacga tatccatatg atgcttggtt tcccgtccga taatagtata tgtttggttg
      361 gcaatcactg ctttgtagcg tgttttttcg tgcgccatac gaatcatttc ctcctatttg
      421 atgaaagttt tatttagtgt accatgaagt tggttctttc accgttctcc acaaatattt
      481 ttcacatact taactattat atgataaact ttaaaaaaat gctatgtctc gcaatctctt
      541 agaaaacgaa aagtcgtgag ttgcggtata gttttatgta taaaaacgag gagaatttta
      601 tgagcaacgt cattatcaaa gcaacaaacg accaacttag tcaaatgaaa gcatactatc
      661 gctcttattt gttaaataaa cagattcctt atactagctt tgctgcaaaa aaagacggga
      721 cgaccattac cgcttataca tccggaaaag tgatgtttca aggtacgaat gcagcaatag
      781 aagctgataa atggggggag gcacaggata aacaagccaa gccagcaaaa acagcctctt
      841 ctttgccaaa aaatatcagt caattatccg ttttaggtag tgatgaggtc ggaaatggca
      901 gctattttgg tccgctaaca gtatgtgctg cttatgtgaa acgtgatcag cttgcagaat
      961 tacgtaaact tggtgttaga gattcaaaag agttaaaaga tagccaaatc atccagcttg
     1021 ctgacatctt aaaaaaaacc atcccctata aactgcttgt cttatctcca gaaaaataca
     1081 atgagatcca accaaaatac aatgccgttc ggatgaaagt cgctttgcac aatcaagcga
     1141 tccatttgct gctaaaaaaa atcgagccag aaaaaccaga agcgattttg atcgatcagt
     1201 ttacgccgga agcaaatttt aaaaaatatg cgcgtttaga aaaaaatcaa ttacaagaaa
     1261 aactgtactt cgttactaaa ggagaacaat atcatttagc tgttgctgct gcttctatca
     1321 tcagtcgtgc cagctttttg gaagagttac acaaagcatc agctgaagct ggaatcaccc
     1381 ttccatcagg agctggaacg aaatctgacc aagtagcggc aaaactattg gagaaaggcg
     1441 gcatgcctat gcttgaaaaa tatgccaaac ttcattttgc caatacagaa aaagctttga
     1501 agctgctaaa aaaataatgg aactgtgaca aaagtcatgt cacagtccct tgttccgaat
     1561 aaacggtgtt ctcatgccaa ctcctcggtc acaagtcaca atagtcatca aacatgagaa
     1621 gctgtttttt gaaattgctc cttgtgacgc ggagttaaac ggcaggctca caacctcttc
     1681 cttaaacggt gttcaaaagc ttgcatctgc gataataagc ccggccaaac gaaaaatatg
     1741 aggagctatt tttgtttggc tgggcttatt tattactgaa aataagtggt caatgcttgt
     1801 gtcaaacttg cagcaatctg ctgctgataa ctgtctgtta ctaattcctt taaatcacta
     1861 tcattattca tataacccat ctcgattaaa atagaaggtt gaagattttc tcgaagaact
     1921 tgatagttcc cagtggagat tcctcttgaa tccagaggca gatcagtgaa ggagtcagca
     1981 attgtctgtg ctaatgtttc ctcttgtcca tagtaataat atgtcgtgat tccagttgct
     2041 tcattcgcat attccgtaga gtctgcgtgt aaactgataa agacatctgc ctttttttct
     2101 tcactgtagt cgcatatatc tccaagctgt actaattcat ctgtagtatg ggtcaaataa
     2161 accgttgctc cagcatcctc tagcgccttt tttactttta tggctgtact taatgttata
     2221 tctttttctt ctgtttgttc atcattgctt aatgccccag tatcatttcc gccatgccca
     2281 ggatctaata caatcactgc atcagatagc tcttccggtt ttgtttgtat attctttgtg
     2341 actttggttc catcttcgag ggctaaatat ccagtatagc catcatttgt cttgatctga
     2401 taagcatttt ttgtttcaga aagaaactcc gccagctctc cgccgtttat agttgcaagt
     2461 ggggtagtta gcgagtcatc tgtatatagt gtacggtcat tttttccgat tttgattaat
     2521 gtctcttgtt ctgtatcatc aaatgattcc tttgtctttg taaccgtact gttcgtactg
     2581 ctgaccgttt gtccaactat ctttttttga ccaaaaacat ctatttgaaa acagataata
     2641 atgataatga ttgcaaatac aaaagctcca attatttttt tcattcttga ctctcacctc
     2701 gtagatccag actaagggtc aaaacttaaa atcagcttaa gtacgcaaaa aaggggctgt
     2761 aaaagatata accgtagaaa ataacattac tgctaaaatg atttttttca tttttggcat
     2821 atcctgaaat ctccttttga ctattcgtgg tgattcatct acccaataaa tagtttcctt
     2881 cattctacaa acaattaatt tcttcgttat taaattaaaa cacttcctgg aacaccgaca
     2941 taataaataa aaatataccc tgataatcat gagagaataa caaatttgta tttatgaaaa
     3001 actacctata taaaaaaaac aagatataac gatcttcgtc atatcttgct tcttcaccta
     3061 actatttttc tccctatata acagctttac gcttctccta atacaccatt caaatataaa
     3121 tcgatcaatt gcgccacatt tagcggttct tccacaggtc ttttcgaccg agtgatgtaa
     3181 ggtgctaaca ctaagaaaaa atgccttgct gcgatttctt ttgaaatccc atttcttagc
     3241 acagattggt ttgctgaaaa gatttcttca aaaggttcac gataattttt cttccataga
     3301 aagaaaagat gatctcttgt ttcctttgaa agattgcttt caagatcgtg aagcatcaga
     3361 aacaaatcga ccagatgtgt ctcaatcaaa aactgcgcca tttttgtcaa ggttacttga
     3421 aatcccgttt cgcttgtttg aacgatttct ctcaaagctt ttccaacatc ttttaagtgg
     3481 ttttctaaaa ctgccgtata tagtttttct ttatcaccga aataatgata gagattgggt
     3541 tgggtgatcc caactttttg tgcgatctct cttgttgaag tgccgagata tcccttggac
     3601 ataaacaagt cagtcgcttc tttcaaaatt ttttgctgtg tatctttgtt gtatcgatgg
     3661 accatttttc cacctgcttt ttggttattc tttttctttc aatacaccat cttccatcat
     3721 aaatattcta tcgcatttgt caatcagccg tgtatcgtga gtaaccatga tcgttgcttt
     3781 atttttgtct tttgattctt ttgccaagat atcgactact tctcgtgcct tttctgaatc
     3841 caaactagca gttggttcat ctgctagaat aatggacgga tcattgtaca atgcacgagc
     3901 gattgcgacc ctttgccgtt ctccaccaga tagttcttct ggatacttgt tgatcaactt
     3961 atccacgcca agctgctcaa ataattcttt tgcttctttt tgacgatgtt catgtttgat
     4021 cttatctact aataataact gcttttttac tgtcagaaaa ggaaccagat tggaagcttg
     4081 caaaataaag ccaatttctt taaaacgtag tttaacgcgt tcactttctt tttgattact
     4141 gaattcttga tcattgataa atacagagcc actgctaggc gtctgcaatc cgcctgccaa
     4201 agtcaaaaaa gtacttttac ctgaaccaga aggaccgatg attgcaacaa attccccttt
     4261 atcgatactc atggttgttt ctttcaatgc tttaactact tggtcgccat cttggaaact
     4321 tttttctact tctttaaatt ctattgcttt catgctcttc ctccttctta tccaattgcc
     4381 ttcaacggat cgatttttac gattgtccga acagaaaata gtgcgcctaa cacagctact
     4441 accagcatca acaagctaat gacgccaaaa aacagccaat tgctttggaa tggaaccgca
     4501 tcaggtagaa caagggatgt tcccactgtt cctaatagac caagaagaat accaacaaat
     4561 gataaaatga atgtttgtgc cactacagaa cgcgcaataa atcctcctgt gatcccttgt
     4621 gctttcatta caccaaagat attaatcttt tgcatcgtca gtacataaat gaaaatcccg
     4681 atcacaatag ccgcaataac gatcaaaaat ccgatcataa atccaaaagt caatacttgc
     4741 gcattatatc caggtaattc attgatgaat ttagaaattt tgatctgttc taaatcttca
     4801 ggtaaattac tgattttacc tctgacaact attgcgttaa tccgtgcatt ttcacttgta
     4861 tcttcttttt cgaagcggat ctcttggtaa gcattgatgg ttgtatagag aactggtgag
     4921 acattgaatt tggcatgatc cgtaaagcca gttagtttaa acgtcttgtc actaccagat
     4981 aacttaactg tatctcctac ggctagacca tattcttctt tcaaactaat atcgcctact
     5041 gcctcatcat cgttatcaaa agcttttcct tcaacaagat tgggcatgat gaattgattt
     5101 ttatcgatac cgaagaagct aacattgatt tttccttctt ctgtactgtc tttactagtc
     5161 acgactccag gtgtctgggc aagatacgcc actttcccac cttcgacttc ttctgctatc
     5221 ttcttagtaa tcatggacat ccctagattt gaatttgcat ctttcgaaag cacaatcgaa
     5281 tctgcctccc acttgtcgac tgctgttcga ttatcttgag ctaagccata agctaaccca
     5341 gtcaaaaaaa ataccaggta ggcgatcaaa aacatcaccc ctgcaactaa cgcataacgt
     5401 aactttgaat gcataatttc atttaatgct aaaaacattt aactcctcct cctgaaataa
     5461 tcattttata caacaatttt atcacttgat aaatttttgg cgaatattat gctcaaaaaa
     5521 ttcttccgct atttcattat atgaattttt tattctcgat tgattaatat ttattatatc
     5581 cccacacaat aaaagaccgt gatatttctc acagtctttt atctactcta ttgctatttg
     5641 ctctcttagc tagtacttta aaagctaact cattgaggta gtgaaaacga gaggtgtgtt
     5701 cagacagaaa tccaaaatcc tcaactgcta gctgtggaat cgtagttttg tgtttcacga
     5761 tatccgcaag caggttcaaa tctgataata agaattcttg gcttatcagc tgtccgccaa
     5821 ttatttctaa tgtatccgga tggtagatca gctttgcatt tactgttttg ttttcttcat
     5881 cgtattgtgg aaatagatca aattctcctg aacacgaacc ggtattttgt tccaagaatg
     5941 gcgcttcatc ttctgtcagc cctacagttc ctaaaaagta gtctataatg actgctgctg
     6001 ttatcttttg tctgaggtcg taagcggttg gctgaccact caaagttagc gctactgctc
     6061 ttgccatatg tacagctcga gtaactaaag gtaaaaagag atgccgttta tttttgtcaa
     6121 aactgactgg gacgatatct ccaatcgcat agatgtccgg atcacttgtc tgcagatatt
     6181 cattgactat cacagtctca tcctcataca aagccacttt ttcttttaac aatgtcgtat
     6241 taggacgagt ttgggcagaa aaaatgacca tatctgcctt tattgcttga gatagaagtt
     6301 gtacggatac tactttctct tctgattttt tccaatcgat caagaattca tttggataaa
     6361 aattgatttg cttagtttgc agctgatttt gaaaactgtt gatcatatcc ttatcgaaat
     6421 atcttgataa aacattcggc atataatcaa ctagatgaat aatgtatcct ttatttctca
     6481 aagcatctgc taattccaat ccaatataac cagcaccgaa gatcactact tcttttgctt
     6541 cttttaaagc ggcaagtact tgcttagatt gcactagatt tttataggtg tagacatttt
     6601 cagcttcaat ttgtttatac aaggtgacag gagaggaacc tgttgctacg attaatttat
     6661 cataggttat ggtctcgttt tttccttctt tggatgcgag gatgagctga tgtttgtctg
     6721 gatcaagttc agttactctc cattcggtga tcagttttgc cccactttct tgaagagtcc
     6781 gcatgttatg ggcaacttca gctaaactac gtatctttcc ttttaaataa aggttgattc
     6841 catttgaaac atagcctagt ccatctttat gatcttcatc gatcagtaaa acttctgctt
     6901 tcggttgaag ttttcttaga tttagggcag ctgtgatgcc tgcatgggaa gcaccaatga
     6961 tcacgatttt catgttttat tcatccttaa attcaatttt tctaaataac attcttttaa
     7021 aaattggttc aaatagggga aatcataatt atattcgttg ctagtgaata caaaaacttt
     7081 agcggacttc tgtgacgggt actctttcaa ttggctagtc aaaatgatat catactgctt
     7141 ttctttctcg tattcgacta aatcatattt gatcgtattc agcaattgtt cctggatgat
     7201 ttctttcacg atatccgcca ttagcctttc ataaggaaga tcataagcaa tttgcagacg
     7261 taattctaac tgcttgatca gcgtccgcaa aatagatgca tagcgagcaa ataattcaaa
     7321 gcgggcattt tcctcgtcta cttcagtaaa atcaacaata gcaaatgttt gtctgaccaa
     7381 tctttcacat aaaaccagga attgtttatc ttctacttca gttaaccgct gttttaatcc
     7441 ttcttggcca aaaatattca tccatcctct aaaataggca aaatgagcgt gattttgcat
     7501 cactgtcaac gcaatctttt gcgaaatctc ttctggaaaa acataaaaag agaaatattc
     7561 ttctatcgat tgtttaaacg ttcgattcaa ttgagctact acgtcgtctt tgtatttttc
     7621 gcttatcaaa aactcctgga catgcgagtt atgtgtatct aatgtaaagt tactgatcat
     7681 gaaaagataa aaaaccaacg tttcattttg attccaagga aaggcatatt gaaagaagta
     7741 acgggtcaaa aatgtatgga ggtccaataa aaattgtgtc gtttgaaaca atccaacagg
     7801 aagattgcta taatctggtt tggatttttt cgtacagcgc attcgcttct tcataatgga
     7861 aagccatatt tcaaatttca atcgtccttc ttctgtaata gtaaaactaa actctttttg
     7921 cagcagatct aaaaaagtgt ttcctgataa atcactgtat tcttttttgt tttcaagata
     7981 aggacgtcca aaccaaaata attggaaata aaaataacaa atttgacttt ctggaccggt
     8041 cagccgcccg ctttttattt tcagatgaaa ctcatttaaa gaatcattta attccttcac
     8101 tctgcgagac aaagtagccg aactgatatt aagttccatg acaagtttct gccgatcaaa
     8161 tgagccattt tccaacagat aaagaagtat ctgatatttt gtagaatagc gcaagtagca
     8221 atgataataa acatcgactg ctgctatatt tccatgtctg attaataaaa tattcccttc
     8281 ttggataaca atttccaatt gtttgatatt ttccaagtta gcttgtaaat aaaacagatc
     8341 ttctttcaag gtagcccgat caactgataa gcgggtaagt acgttattaa cggaaatctc
     8401 tcctttttcc tctaataaaa gttggaaaag tttgatttgc cgttgttcat ttttttcaag
     8461 caaactgaaa agttccaagt ccactcctcc aagcatttct gattttaaca gcagaagttt
     8521 ccttctcatc cttgaaaaag aggatctgtg acagttatca cagatccata caaattctaa
     8581 tcacttagct ggttggccca gctgtcagtt cccatgtgac catgccttga tacgttgcgc
     8641 caggttgagc attcgtactg ctatttacct ctaagagtaa accatcggta tcgggaaagt
     8701 caacgagaac ctctccttta gctgttccat cactctcata aatcaacgtg ttttgacctt
     8761 gctgaatcaa ttgttttgat ccagaatgat tgaagtataa ttttgttccg cttaattctt
     8821 gaccttgatc attcttaaat aaagaagtca atgtccctgt cagttgccaa ggagacgtgc
     8881 ttggaagccg agtatcccgc acatcaaaag aaaaatcgcc ttttgcttca taataacttg
     8941 tcttcaacgg tttggggaga tcgttaccaa atgtaaaatc atctggtaca ttaactaatg
     9001 ttaattcttg tttggggaca agaattgttt tagcagatgt ttggacaact gggttaggaa
     9061 gcttagcacc atctgctaat gtaatattgc tggaaagtac agctgccttt gtcagtatgc
     9121 tgacggattg tacagaagta tctacagtat ttaattgata gttgtaagta agatgaatgg
     9181 tatctcctat tttgctcaat agatctgctg ggacagaaac tccttgttga atgccggtat
     9241 ctgcgatagt tgtaccattt aattttaagg aaccattgat ataagtagta ttttcaggaa
     9301 gtgagatcgt gatttttttg tcggttatcg catccccaga ttttgttaac gtgaatgtca
     9361 cattttcttg aatcgtatct cctgatagag cagacagggg atctcctgtc tcaggttgtc
     9421 tgctaaggtt ttttagctga ctatctgcac tcacagatgg aaaattagcc actgttattt
     9481 gaggaagagt ggtgattcca gcttgttcag actgccattt gtacgcaaaa gacggataca
     9541 tgttagtaag tacatcgtcc accaagcgat aatcaaaata atattgccct gaagccaatg
     9601 caggtagtgt agaagtagct gtatttacac ccccgttatt tgattggatc gtttggaaaa
     9661 gttgatctgt ccatcctgtc gctaattttt gggttccttt cgaataaagc ctgtaatgga
     9721 tcgaaccctt ttttagttca gttgctgacc aattggccgt gacttggtac gatgcagaag
     9781 aaaggagctt tccccactga ctatcggctg ttaagtttgt tggtaaaacc gttccgtcta
     9841 gcttgtactg ctccaatacc tttaaagcaa caggagaaag caatccgcct tgcgcatctt
     9901 tcccactgta cacgatattg gctgtctgat tcaatccact tgtgcttgct ggagtatgag
     9961 tcagtattgc tccttgtgta acgttttttc ctgtaaaata gttgactacg cgatcaaagc
    10021 gactaaatgg caactgatca aaacgtcctt cagtcagccc catcgtcaat gtccgactat
    10081 tggtagagcc tggaaggttc tggttatttc catccacgta ttctgtggta tcagcaaatg
    10141 tattatattt tcctgttgaa tcacttgttg agatgtaact attccacgta tttatctctg
    10201 cacttgcaga aaatgcatat gctcctggat tcaagtttcc gccagttaat ttaggataaa
    10261 atctacctgc agctggagct aaaatcgaat tgaacatacg atcttggata atattattcg
    10321 aatcataaaa atcaacagaa aaaacggcct ttttagacat agaaggttgt aatttaccct
    10381 tggtcaaaat tttgtttgga ctcgtatcat atttatctcc gttttctgtc atcttcgcga
    10441 cattagctgt aacatcaaat tgaagcggga tatcgtccac gttgaattcg cctcgcgtaa
    10501 taatagcaat aatatctgcc aatagagtat ttgtatccca cgaccccatc ccaaaacgca
    10561 aggtatggct tcctgctgat aataaactac cgctagtaac cttgatgggg gcaccggcaa
    10621 tatacaagta ggcgttgttc cagtcaattg cttttaatac atcactgctt gatacatgtt
    10681 ccggcaaatt gatgatcgcc acaaatgcaa atccttgtgc agtgatattc accaaatcaa
    10741 gtacatttgt tagtttcaca tggctttccc aatgaaaacg gaccgtgcca cctttccaag
    10801 gatcatttga accatcgttc aaataagtag cagtcaattt aggcaattgg ttagccccag
    10861 ttagttggac aagcttcccg ccattgactg cactacgtcc accatttgga ggtacgatat
    10921 tttcatacgt gtatggatca taagaaatgg caggaggcgt tgctccaaca gcttctgccg
    10981 aaggtgaaaa gataaatata cttaaaacaa aaagcgaagt aaaaaagaag gagcgaaaaa
    11041 atgaaaattt attttgtagc aacatgttca tcctcctttt cctttcttct ccataacaac
    11101 aaccacgcaa gaagccataa ttctatacca atgatcaaat aaagaaaacg gatcttttct
    11161 tcattcgtct ccgggaatac gccatgttga ttagattctt tataaatcgg tagctgtgtc
    11221 tgctgttttg atgcgccatt gactgaacca cttggatgcg gaactggact tagatcataa
    11281 aatgtaatcg tacctgaact tttggtatct gcttggactg gctcttgcca gctgaagaac
    11341 agcaaacttg caataatccc tcccaataag atcgatgttt tcaacgaaat cacctcatca
    11401 tgttgtttct ccttttttct ttttcttgtg gacccacaaa aacagcaaaa tcaaccataa
    11461 cactagaact gcagcaacca atcctgcata aagccatggg aaagattgtt cttgtactgc
    11521 aaaaacggaa ttttcattta ctttttgtgc tttttctttt ttgatcgtaa agttttgtgt
    11581 aaatcgccat tcattactgt cgcctgattt tgctatgatt tctgcgcggt aatcgccggc
    11641 ttttgcttta tcaccagcta aatcaagatt caagtgaaaa ttcgaatttg gtgcaactaa
    11701 aaattcatta gacgtatttt caatataaag ctgattttca taaaagatct tgctcgtaaa
    11761 tatcaaattg ctgataatcc taggctgagg attttggaaa cgaagctgaa cagttggctt
    11821 tgcgttgacc tcgtccaaat ctcctgataa aagtttcaga tccggttcta tcgtctcttg
    11881 actttcttgt aaaacgactg cgagattata agcaatacga tttttgattt gtgctccttc
    11941 tgtagcattt tctgtttcag cttcttcttt aggtgaaaca tgaatacccg ctaatacacg
    12001 tcccttaaag ctttttgctg ggattttcac ttctataggt atagttgttt ctgcatgtgc
    12061 tgatacattg atttcctttt tcgcaatgag caaaacgtca cggacatcaa acggcaatgt
    12121 ctcatctaac gttgggtttt tttgactata atcgatcgtt ccaccatcac tcgttaccgc
    12181 cggattgaca gagacctcaa atgttcgttc ttcatctgaa gaattgctga taattacttc
    12241 tagtgtctga ttttgttccg gtgccactaa taaatcaaaa taaccgattg ctttatctat
    12301 ttggttctct ggaaataccg gtttgattcc aaagtctcct gctgaatcct ttgcttggct
    12361 gattgacgga aaaatgaaaa atagacttaa aatagaaaaa aacgaaacaa acagtcgttt
    12421 actttgattc attgtccacc acatcctttc aacaacaact gcggtaaaaa atccttcacc
    12481 tatagtattt tcaccgcagt ttgttgaatt tatttcatac tctatgctac tgggccagcc
    12541 acgaggttcc aagtcaaatc agccgtgaat gttccagctg ctgctttcgg cactttcagt
    12601 gtgatatttg ctggatcata aacagaaagc catgttccca gaccttgatc ttttgctgct
    12661 gtaaatattg gtgtcgctgc acttgcttta ctcaacccag ttacagtttg actagttggt
    12721 ccagtggatt cgttatctga cgtactttta acagtggatg ttccaaaatc taattcagcg
    12781 ccttgcaaaa cttgtgaacc gtttacaaaa tctgaaaccg aaactgtcac gttccaccct
    12841 tgtgcttggc catctgcacc tacacctcga cgatctgata cttggagata aggtgtatcg
    12901 ttttttgaag cgtctacttg atacgttttt gttccgcttt caatctcgtg tgtaccaaat
    12961 ggcaattcag gaactacatc caatgttaaa gaacctgtct ggccagttgc tggattttcc
    13021 ggatctgctg gctctactgg ttttgaagga tcagtagggt taagaggctc aacggtattt
    13081 gttggttcag taaaggtaat atctcctttt gaagtcgctt gaccctcagc aaaagctggt
    13141 gttgccaagc cgcctagaag aagtgttgaa agtaataatg tcccaatcat gttctttttc
    13201 ataataaatc actccttttt cttagtgacc taagtatagt agagacagta tgcaaaaaat
    13261 cctcatgaat tcatccgctt acttgaaatt atgacccttt ttgtataaaa gagaaaaaaa
    13321 caataaaatc gtttacatga atattcttcc ttttttatta agtatgaaaa aatatttcaa
    13381 ttttattttt tcgtttcaaa ttattctatt agtgacatcc ttgaactata aaaataggag
    13441 tacggcatca tctgccgcac tcctattttt ttcgtcattt atttcttaaa taaaacgatt
    13501 ctcacgcaat tttatttttt tgatatcttc tactgtttgt gttccagcta attgcatgac
    13561 catttccaat tctgttttaa agaaatcaaa cacttgatgg actcctgtac ttcctcctaa
    13621 agacaatcca taaataacgg gtctgccaat agcaacaaga tcggcacctg atgcgattgc
    13681 tttgaaaaca tgttgtccac gtcgtacacc gctgtcaaac acgattggta cacgtttatc
    13741 tactgcttct gccacatatt gcaacgaatc aaacgcagca ggccccccat ctaattgacg
    13801 gccaccatgg ttagataccc aaattccacc agcaccagat tctaatgatc gatagacatc
    13861 ttcttctgat tgaacacctt tcacgtagac tggcaaatca gaatgagcag cgataaattc
    13921 tacatctttt ggactaagtt tttgtttgga agatttatat acagcatcca tcgtttggcc
    13981 tactcctgat tgataagcct gaacgatcgg cattggcagc gggaaggtaa acccgtttct
    14041 gcggtctgtt tcacggtttc cgccaactgt tgcatccgct gtcaatacga ttgcttttgc
    14101 tccattacgc ttggccactt caagaatatc caaattgatt ccattatctt tgctcatata
    14161 aaattgaaac cattgcggtg ctttctcccc gcctgcttct cttatctctt ctaaagtaca
    14221 agaagcgtaa gaacttgctg tatagatcgt cccaaaatct gctacacctt ttgcagaagc
    14281 tttctccgcc ttcacatgag ctaatccatg agcagcaact ggtgccatga tgattggtgc
    14341 agtcagcatt tcttcgtcaa aatgtgtagt tgtatcgggt aattctacat ctcttaatac
    14401 atgaggaata atcaaacgat gattgaatgc ccgttcattt tcttgataag taaataagtc
    14461 gccagcacca ctgctgatgt aaccataacc accagctgga atcacttgtg aagccgcgat
    14521 ttctaaatct tccatattga taaaatctac tattccttca tttgtacctg cttgatatgt
    14581 tttttccatc tcaatgcctt ctctctataa aatttgaatg atgaacaata tgatagcgtt
    14641 tacacttttt atattactct tcgacattga tacaggcaaa taatctgttt acatttcagc
    14701 cattccagat gtcactactc tggaagaaat gattgtaata aaaatagata aaaaaccgat
    14761 gggactttga caaaagtagt gtcaaagtcc ctttttttga ataaacggtg ttcaaaagcc
    14821 attttctcgg tattaagtca aatttatgca aaaatgtgga aagccatttt cacaaaattt
    14881 ttcttactac tcgaaactaa gcgacttttt cacaacctct cgat
//
LOCUS       DACGLW010000073        14666 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.076, whole genome shotgun sequence.
ACCESSION   DACGLW010000073 DACGLW010000000
VERSION     DACGLW010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14666
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.076"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            126..1418
                     /locus_tag="IXT53_002199"
     CDS             126..1418
                     /locus_tag="IXT53_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ4905996.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIY
                     TAQECIHCKHQIASDIVRWGTTTVRLLMNDVSEYRTYLELKKQRFKCKACQRTFVADT
                     SVAKKHCFISEKVRWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRTP
                     LPKVLCFDEFKSVRGVSGAMSFIMMDGQTQRLLDIVENRQLPFLKRYFSHFSREIREA
                     VEWIVIDMYAPYVSLVKKLFPKAQLIIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQK
                     HYQLGKQLKRYWKLLQKDERKLDYTRRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQ
                     LYQDFLYAVKERDYVSFEELLTNNIMLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPL
                     ECLNNHIKVLKRNAYGFRSFYNFKLRIMIRHGNALIFN"
     gene            complement(1661..3781)
                     /locus_tag="IXT53_002200"
     CDS             complement(1661..3781)
                     /locus_tag="IXT53_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HAQ4905997.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQLEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHKG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            4011..5189
                     /locus_tag="IXT53_002201"
     CDS             4011..5189
                     /locus_tag="IXT53_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HAQ4905998.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            5205..5963
                     /gene="aroD"
                     /locus_tag="IXT53_002202"
     CDS             5205..5963
                     /gene="aroD"
                     /locus_tag="IXT53_002202"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HAQ4905999.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            5986..6471
                     /gene="ybaK"
                     /locus_tag="IXT53_002203"
     CDS             5986..6471
                     /gene="ybaK"
                     /locus_tag="IXT53_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HAQ4906000.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSHIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(6542..7795)
                     /locus_tag="IXT53_002204"
     CDS             complement(6542..7795)
                     /locus_tag="IXT53_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ4906001.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            7911..9260
                     /gene="gdhA"
                     /locus_tag="IXT53_002205"
     CDS             7911..9260
                     /gene="gdhA"
                     /locus_tag="IXT53_002205"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HAQ4906002.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNAQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            9372..10718
                     /locus_tag="IXT53_002206"
     CDS             9372..10718
                     /locus_tag="IXT53_002206"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HAQ4906003.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(11026..11412)
                     /locus_tag="IXT53_002207"
     CDS             complement(11026..11412)
                     /locus_tag="IXT53_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ4906004.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYTEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            12291..12503
                     /locus_tag="IXT53_002208"
     CDS             12291..12503
                     /locus_tag="IXT53_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906005.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPTELVS"
     gene            complement(12781..14580)
                     /locus_tag="IXT53_002209"
     CDS             complement(12781..14580)
                     /locus_tag="IXT53_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAQ4906006.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRSFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
BASE COUNT         4802 a         2678 c         2860 g         4326 t
ORIGIN      
        1 gctctatact ttttgatgtt ggtggatgac accagcatct tttttatttt ccaaaacata
       61 tagaaaagac accttgaaat cctttagaat taatttggcg aaccaaccca aaaggaggaa
      121 tcaagatgtc ttacactcac cttatcaaag aaacattaga tattctagac ttatcagtta
      181 cttttaacga aaattgtctc acaaaagaaa aatacaaagg gcaaatctgt catatttatc
      241 gtggaaactt gatttataca gcccaagaat gcatccactg taaacaccag attgcttcag
      301 atattgtgcg ttggggtaca acaaccgtcc ggttgttaat gaacgatgtt tctgaatacc
      361 gtacctacct cgaattgaaa aagcaacgct tcaaatgcaa agcttgtcag cggacctttg
      421 tcgcagatac ttctgttgcg aaaaaacatt gtttcatcag tgagaaagtc cgctggtctg
      481 tcgtgactcg tctgaaaaaa aatacctcaa tgacagagat cgccgctcag aaaaatctat
      541 cggtctcttc tgtttaccgt attatgaagc gattttatcg tccgttgaat ccttttcgga
      601 caccgttacc taaggtactt tgttttgatg aattcaaatc agttcggggt gtttcaggtg
      661 ctatgagttt catcatgatg gatggacaaa cacagcgctt attggacatt gtcgagaacc
      721 gacaactgcc ttttttaaaa cgttatttta gtcatttttc acgagaaatt cgtgaagcgg
      781 tcgaatggat cgtgattgat atgtacgctc cttatgtatc gctcgttaaa aagctgtttc
      841 caaaagcaca gctgattatt gaccggtttc acatcgtcca acatattggt cgtacctttc
      901 gcaatcatcg aatcaaagaa acgaaccaac tattaaaaag caaagaacaa aaacactatc
      961 aattaggtaa gcaattaaaa agatactgga aacttcttca aaaagatgaa cgcaagcttg
     1021 attatactcg acgcctttgg cgacctggct tcaaagctca tttaacagag accgatattg
     1081 tggatcgctt actgaaaggg agccctgctt tgcgtgtagg ctatcagtta tatcaagact
     1141 ttctttacgc tgtaaaggaa cgagactacg tatcgtttga agaactactt accaataata
     1201 tcatgcttcc cgaaggctat cagacgacct tgagaacgtt ccagaaattt ctgccacaaa
     1261 tcaaaaatgc tttacaacaa agttattcaa atggcccact tgaatgtctg aacaaccata
     1321 tcaaagtgtt aaaacgaaat gcatatggct ttcgtagctt ttataatttc aaattgcgca
     1381 tcatgattcg tcatggcaac gcattgattt tcaactaaaa aagatcaaga ggaactgatc
     1441 cacttgatct ccaattattc taagatttct ctttaccaac atcatttgac aaagagccca
     1501 aaaaatctga gccagaatga ggttcattct gacccagatt tgattttgta caacccatca
     1561 gtccttattg acaaagagcc aattattcta agatttctct ttaccaacat catttgacaa
     1621 agagcctaat tttgtcgctg ctccttttaa atttatttta tcattcggaa gaacttgtgg
     1681 tcgttccgtg aagttcttgg tacgtttgcg ttttataaag atcaacagtt gattgtccct
     1741 tgtgttcact aaagacacta gttgacttgt caccaagaga tttttcagct tcttttaatt
     1801 tctctaagcc attcttgtag tcataatcac tggtatcaat tgggtctaat ccactattcg
     1861 tatagaaccg aagaagatca ccattattga tttggtcaga aacagctaac tgtttatcaa
     1921 ctttttcttt cagtgaatca atttcattct gtacttcctc agtaggttca gtaatagctt
     1981 caccagtttc gttactatag atcgtaccac cgtagtaagt atattttggt gtaacaaaat
     2041 caccatcacg gaaagaaaca agctgttcat gatctttcga gaatagatct tgtcctaact
     2101 gaatataatt ttttgtatct acacctagta agtgaagtaa tgtaggcaag gcatcaactt
     2161 gtccgccgta agtgtgattg attccgccct gtgtttgtcc agggatatgg atcatataag
     2221 ggacacgctg catttgtgca ttgtcgtagt ttgtccaagt agaacttgtt tttccaacta
     2281 attcagctag atttttattt cttgaattcg agacaccgta atggtcaccg taaaggacga
     2341 tgactgaatt atcatacaag ccgctttctt tcaaataatt gaagaattct tgtacggcgg
     2401 tatctaaata attggctgtc gcaaagtaac cgttgatcgt ttcatcattt gtattggcta
     2461 acgggaatcc tgcttcgtca ttggtgaatt ttgaataagg ataatggtta gatacagcaa
     2521 tgaattttga ataaaatggt tgttgcagac gttccaagta ttgtacggat tgttggaaaa
     2581 atggtttatc atgcaacccg tattggaacg aattgtcatc attaacatca taataagaag
     2641 aatcaaagaa ataatcatat cctaaattct tgtatgtctc gtttcgattc cagaatgtac
     2701 cagcgttccc atggaatacc gcactagtgt aaccttgtgt ttgacttaaa atatcaggtg
     2761 cagcttggaa agtatttttt ccgcctaatt gtgtgaacaa ggagccttga ttcaagccaa
     2821 acaatgaatt ttccatcagc gtttccgcat cgcttgtttt tcctgctttt acttgatgga
     2881 aaaagttgtc aaaactgaac gtgctgttgc tgtggtaaag actattgatg aaaggtgtaa
     2941 cttcaagctg gagaccattc tcatctgtca atttataatc gatcaggaat tgctggaagc
     3001 tttctaaatg gatataaatc acattttttc ctttagcgag cccgaacaaa tcatcatttg
     3061 gtgctgcata atgttctttt acataatttt ctacttcttt catatcattg ggacttgctt
     3121 ctgcccgcac ttggtttgtc tggtaagttt gaacaccgtc ataaaccatg aaagcattca
     3181 agccaagata tttgaccaaa taatcacgag agaatgttcg agtcagcaat tctggacgat
     3241 cagcttccgc cataaaaaga tttcctgaga aaatcataac tgctaatgta gagatcgcaa
     3301 aagccattct tgctcggatc gggcgagggt ccaacttgat tttttttgca atcagcagaa
     3361 cagtcaagat aataaaatcg atccaataga tcacatcata tgggcgaaac agtcgaatgg
     3421 cactttctcc cagaccactc gctactttcc cagcacctaa aatcgtgttg atcgtaatga
     3481 agtccgtgaa ttctcgataa taagtgacat tcgaaaaaac aagcaatgtc aaaagaaaat
     3541 aaatgaccat catcgtgata taagcaactc tagggcgtct gatataaaga gcaattccaa
     3601 gtaataacaa tgtagttgca atagggttga taaacaagat aaagtattga atgccacttt
     3661 cgattccgag atggaaatca actaaatagg caaaaatcgt tttcaaccaa agtaaaacaa
     3721 ctagtaaaga aaaaaatccg agcctcttat tcaaaaaggc aggtgttttt atacatttca
     3781 agataatctt cctttctgca cttcttaatc acagaagtgt catgaaacca tgttacacag
     3841 ctcacttatt ctcatttatt ttactagtat gacaatatag tgtcaattga aaccagaagt
     3901 ttatgctgtt tcattaaggt tttctttaag cttcatgaaa aaatgagcag taagacaagt
     3961 cctatgtttt ttgttatact tgggaagaga aagctaaagc aggtgaagga atgaaagtaa
     4021 aagtaaaaaa aagagcaagc aatcgattga aaaaaggata tccactgatc caaaaagaag
     4081 accttgctca tccccaaaaa gaaaccacgc aatgggtgga attagttgat tcgcaaaacc
     4141 aatttatcgc aaaaggatat ctaggaaagc agaataaggg aatcggttgg gtattgagcc
     4201 agcagaacat cccatttgat caatcttttt ttgaagaatt ttttgtgaag tcaaaagaaa
     4261 aaagaacttt ctttttttca gatgattcta ctaccgcttt tcgtatattc aatggagaag
     4321 gagacgggat cggcggtctg attgttgatt attatgcagg ttatgctgtt ttttcttggt
     4381 ataacgaaac gttatatgca catcgtgaat tattgattca agcttttcgt gaaatctacc
     4441 cggaaatcgt aggaggctat gagaaaattc gatttgaatc agtaaatctg cctgagagcc
     4501 agttcattta cggaaaagag gcaccggaac ccttgatcgt aaaagaaaac ggagtaaaat
     4561 ttgccgttta tttaaatgaa ggattaatga ctgggatctt tttagaccaa aaagaagtac
     4621 ggggatcatt agcagaaggt ttcgcttctg ggaaaacagt tttgaacatg tttagttata
     4681 ccggcgcctt ttctgttgct gccgcaatgg gaggggcaat cgaaacaaca agtgtcgatc
     4741 tagcaaaacg cagtttaaaa aaaacacagg aacagtttga agtgaatggt ttggatgtcg
     4801 atcagcaaaa aatcattgtt atggacgtat tcgagtattt caaatacgcc aaaagaaaac
     4861 aatttttata tgacatgatt gtattggatc caccaagttt tgccagaaat aagaaaaaag
     4921 tctttcgcgt agcaaaagat tatggggaac tggtcaagga tagtctggat attttagcag
     4981 aagacgggat acttattgcg tctacaaatg ctgcgaatgt ctcgatagaa caattccaaa
     5041 caatgattga agaggaattc catgccaaaa atgtttcttt tgaacaactt gctttatatc
     5101 gtctgccaaa agatttccag acgacggata cctttttaga aggcaattat ctgaaagtgt
     5161 ttgtttatca aattaaaaat aaccattaga aggagcgatg cagaatggtt tgggtaaagg
     5221 gattgaatat aggaagcggt agaccaaaga tttgtgcgcc tcttatcggg gccgtccgag
     5281 aggaagtatt acgtgaagca gttcttgcac gtgaagcagg ggccgattta gtcgaatggc
     5341 gagtcgatca ttatcgtgaa gttttttctt ttgatgaagt agcagaaaca ttggttctta
     5401 tccatgagat gcttgatgga ctgccgttat tatttacgtt tcgtacctta gctgaaggcg
     5461 gagaacaaga catatcgatc cgtcagtatc gcgaccttta tgaacatgtg atacacacag
     5521 gattagtaga tatggtcgat atcgagttgt tcaaagtaga aagtctagga aaaagactga
     5581 tagaggaaat caagtcttta aacattcctt taattatcag tagtcatgat tttaaagaaa
     5641 caccagcaga ccctgtcttg ctttaccgat tgaatgttat ggaacatttt ggagccgata
     5701 tcggaaaact ggcagtaact cccaataatg aacgagatgt tttgcggttg atggagttga
     5761 ctagacgggc aaatgctttt gtctccatgc ctattattac gatgtccatg ggaaatcttg
     5821 gtaaaatttc tcgtctggct ggcggaacga cgggttccgt tatgacattt ggttccttaa
     5881 gcagcgattc tacttctgct ccaggacaat taccggtcaa agcattgaag caaattattc
     5941 aattattaaa tgaaccgttg tagaacaagt ttgaggtgat attttttggc aaagaaaaaa
     6001 atagtaaaaa cgaatgcaat ccgtatggtc gaacaaaaaa agattcccta tacagagcac
     6061 gaatatgagt gggacgaaag ccacctaagt gcgtcatcgg tcgctgaaca gcttccagaa
     6121 agccaatctc atatatttaa aacactggtt gctgtgggca atgtgacggg gcctcttgtt
     6181 gctgtgatac cgggagaagc cgaattgaat ctaaaaaaat tagcaaaagt ttcagggaat
     6241 aaaaaagtag aaatgctcca tttaaaagat ttggaagcaa cgaccggata catacgtggt
     6301 ggctgctcgc ctataggtat gaaaaaacta tttcctactt atttagatca aattgcagaa
     6361 agctatgaac aaatcattgt ctcggctggc agaagagggc tacagatgga gttagcacct
     6421 caagatatct gtgcattaac gagcggacaa tttgcagata taaaacaata aatcatacgt
     6481 ataaaagaaa gaaggaggcc gggatgttgt ttgtccagtc tccttctttc ttttttatac
     6541 attatgctct ttgcccggca ttcattcctg ctacatgccc tgtagcgaaa gccgctgtga
     6601 tattaaagcc gccagtataa ccattgacat ccagtaattc tcccgcaaaa tacagaccat
     6661 caatgatttt actctccaat gttttaggat aaatttcctt tagagaaacc cctccgccag
     6721 ttacaaatga tttctcaata ggaaaagttt tgttgattga tagcttaaac tctttgcata
     6781 gttgaacaaa atcttgaatt tgtttttgag aggcttgttg tcctgtcaga tgatccattt
     6841 caagccgctc aagaaagaaa actaacaatc gctcaggcaa tataccatgc caagcattga
     6901 ccaagttctt tttggttgct ttactttttt ctgtcaatat atgaataagc tcttgtttgg
     6961 tttgtgtagg gaaacaatcc agagaaagtg tcactggttc tcccgttttc tctaattctt
     7021 tattgacaaa acttgaacaa cgcagtgcag ccggaccaga aataccgaaa tgggtgaaaa
     7081 gtaaatccat cgtatgttca gtcaatacac gacctttttt atttaaaacg cgtaaagtga
     7141 tgtcttgtaa agaaagccct tgaagtgttt tctcctgaat atacggttca tccgatatca
     7201 agggggactc tgtcgcataa agaggcgtca ccgtgtgacc cactcgcttc acgatcttgt
     7261 acccatctcc agtagaacca gtagatggat aagtccgtcc accagttgtt aagataatgg
     7321 caggagcatg gaactcttcg aattccgtgc gtataccgta aattttatgt tctttgtgga
     7381 tcaatttctc tactcgagta gagaatagaa gcgtcacatc tagttctttc agacgattga
     7441 gcaatgcttc gataatcgtt tttgatttat ttgttacagg aaacatccgt ccatgatcct
     7501 cttcttttaa atgtacgcct tgtgattcaa agaagttcat gatatcgaaa ttattccatt
     7561 gcgcaaacgt actatataaa aactttccat ttccaggaat atgtgcaatc aaatcatcaa
     7621 caggacgatt gttcgtaaca ttgcaccgac caccacctgt catcagtagt ttttttcctg
     7681 cttttttgtt tttttcgatc aagagtacgc gtgcgccttg ttcagctgca ctgatcgctg
     7741 ccatcatccc gcttgttcca gcgccgacta cgatcacatc atacagtttt tccattccct
     7801 cacctcaagg aaagtgtagc atatctgaag aaagaaaaca gaaaaaatgt tttgagccaa
     7861 aagagaaaat gagctaaaat gagggaagat aagcaaagga gcgtggaacg atgacaaacg
     7921 caacagaata tgttcaagca attcaagaaa agctacatca aaaggatcaa gggcaagaag
     7981 aatttttaca agcaatcgat gaattcatgc ccacggttat gtcttttttg gacactcatc
     8041 ccgaatatat cgaaaagaat atcttagaac ttttaacgga accagaaaga gttattcaat
     8101 ttcgagtgcc ttggcaagat gatgaaggaa actggcgagt aaatagaggg taccgtatcc
     8161 aatacaactc agctatcggc ccttataaag gtggattgcg cttccatcct agtgtcaatc
     8221 taagtatctt gaaatttttg gcttttgaac agattttcaa gaatagttta accggtctgc
     8281 caatcggtgg aggaaaaggc ggaagtgact tcgatcctaa aggaaaatct gataatgaga
     8341 tcatgcgttt ttgtcaaagt ttcatgctgg aattgtcaaa acatatcggc ccatcgattg
     8401 atgttcctgc gggagatatc ggtgtaggag ctagagaaat cggctatttg ttcggcgaat
     8461 ataaacgatt gaaaaaatat gatactggtg tgttgacagg taaacctttg gatttttggg
     8521 gaagtaagat acgtacggaa gctactggtt atggattagt gtattatgtc aaacatctat
     8581 tgaacgaaga aaaagattca tttaatcaaa aaacagtttt tgtttccgga agcgggaatg
     8641 tagccattta tgcaatcgaa aaagtgcaag agctaggcgg gaaagttgtt acatgttcag
     8701 attctagcgg gtacatctat gatccagaag gaatagatgt gaaactattg aaagaagtca
     8761 aagaaatcaa tagacaacgt ttgacggaat acgcaaacca acgtccttct gctatttatc
     8821 atgctggaga atctgtttgg acattgaaag aaaaagcgga tatcgctttg ccatgtgcta
     8881 ctcaaaatga aatagacgaa agcttggccg aaatcttagt agaaaatggg atcaaaatcg
     8941 tagcagaagg cgctaatatg ccatgtacag taaaagctgt ttctgttctt catgaagcag
     9001 gcgtgtggta ttgtccagga aaagctgcta atgctggagg agtagcagta tctgctttag
     9061 aaatgagtca gaacgcccag cgattgactt gggaaagaga gcaagtggat gagcagctgg
     9121 atcaaatcat ggaaacgatt taccatacgt gtcgccaaac agcagccgag tacggcgacg
     9181 aaaaaaatct gcttcttgga gcaaatgttg ctggatttga aaaagttgcc aaagcaatgg
     9241 cagcagaagg tttagtttga aaaatgaaac tttcttggaa atattgtcag cattttgttt
     9301 atttgcttga aaaataataa aaaaaaacgt aaagtattac ctgtagagta aacctataag
     9361 gaggagaaca aatggcacac attcattttg actattcaaa agtagcaccg tttgtcaacg
     9421 aacaagagtt agggtacatg caaagccaag taacagcagc ccacaacgaa ttacgggaag
     9481 gtactggcgc aggaagcgac ttccgcggct ggatcgattt accaacgaac tatgacaaag
     9541 aagaatttgc acgtatcaaa gaagcagcta aaaaaatcca atctgattct gaagtattag
     9601 ttgttatcgg tatcggtggt tcttatttag gcgctcgtgc agcgatcgac ttcttgaatc
     9661 acacattctt caatttatta gatagcaaca caagaaaagc accacaaatc ttctttgccg
     9721 gaaattcaat cagctcaaca tatattgctg acttgatcga agtaatcggt gatcgcgact
     9781 tttcagtaaa cgttatttca aaatctggaa caactactga accagcgatt gcgttccgtg
     9841 tattcaaaga gcttcttgtt aagaaatatg gacaagaaga agcaaacaaa cgtatctacg
     9901 ctacaacaga taaagcaaaa ggtgcagtta aagtagaagc agatgcagaa ggttgggaaa
     9961 ctttcgtgat tcctgatgac atcggcggac gtttctctgt attaactgct gttggtttgt
    10021 taccaatcgc tgttagtggt gcagatatcg atgcattgat gcaaggtgcc gctgatgcaa
    10081 gcaaagcata ctcaagcgac aaactagaag aaaacgaagc ttaccaatac gcagcaatgc
    10141 gcaacattct ttaccgtaaa ggcaaagtaa ctgagttgtt gatcaactat gaaccaggaa
    10201 tgcaatactt ctcagaatgg tggaaacaat tgtatggtga atctgaagga aaagaccaaa
    10261 aaggcatcta tccttcaagc gcaaacttct caactgattt gcactcttta ggacaattca
    10321 tccaagaagg acgtcgcaat atttttgaaa cagttgtgaa ggttgaaaaa cctcgtaaat
    10381 caatcacaat tcctgaacaa gcagaagatt tagacggtct aggctacttg caaggaaaag
    10441 aagtggactt cgtaaataca aaagcatttg aaggaacatt gcttgctcat acagacggtg
    10501 acgtgcctaa cttgctagtc aaaattccag aaatggatgc ttatacattg ggttatacaa
    10561 tgtacttctt tgaaattgct gtaggtattt caggttactt gaatggaatc aacccattcg
    10621 accaaccagg tgtagaagct tacaagaaaa acatgttcgc acttctaggc aaaccgggct
    10681 ttgaagatct tgctaaagaa ttgaacgaac gcctgtaaga taagggtttt tcgctagatt
    10741 taaataatca gtgatagaaa catgttcgct ttaaaatgaa aactggtacc catttaggta
    10801 ctcaaacata ctgcatcgtt tattcagaat aaggggctgt gacaagacgt ttgtcacagt
    10861 cccttgtttg tatagaaatc gattgattgc cttgctcttt agtttgtttt aggactttaa
    10921 gaacagtgag gctgacttcg aaggttaatt ctgttttttt gttttctaga atcagtaagc
    10981 aaaaaagcac tcacttcgag tgcttttttg cgatatattc ttagcttaag ataggcctaa
    11041 tttttctttt acagatgagg gtaccttatc gctggtaatt acttttttct ctatgatccg
    11101 cttttcgctt gctttcaact gcaagatcgt tccttcggtg taagcagaac cattggcaga
    11161 gtcaccagaa gtaatgacat cgaaatctaa ctgtcttttt tcaccgtttt cagcataagc
    11221 atctacgttg tatttaaaac ctcttccaat tacttcccca ttatctgctt tgatatcagt
    11281 ttctgcaggc attggagctt tgactaccgc ataataatct ttacctacat aagtagactg
    11341 atagtagtcc caacctttcc atcctataaa agcaatcaat atgataacga tcaaccctaa
    11401 aatttttttc atgaaagatc tcctttttta tttttattat attaaatatt atacagataa
    11461 taacggaata ttatcatcga aacagctaac aaaattgtca taaaaaacga gatggttgat
    11521 attagaactt gtgtttttgt ttaggtctag agataaagag agacaatttc caaatatatg
    11581 taatgaatac aaaaagacgt ttgtcgcgga ttaccgttac aaacgtcttt tattgatttt
    11641 ccgagatctc atcagtcgcc atttggaatg gttcactcct tatctagatt cgtttaatgc
    11701 tcggactgct tgttcgattg taataccaaa cgcaacgtta ttttcgtttt gtgcatcaat
    11761 cgccataaaa cggttagaag ttgtatcaaa agctacgcgg tattgcatat ttgtttttgt
    11821 aaacgtcata aaattcatct cctttgtaat gtaatttagt tcttgaaagt ttcctttaaa
    11881 ctttcgaact tataccaagt ttagcatcgt acgttttttg atgcaattaa aaagcattta
    11941 tcattttcct tatttatttt ttcttaaatg gtttagctat tttcttttaa tccgataagt
    12001 agcccaatca tcaaggttac aaatactatt gcttgaatat aaatcatatg ctcacctctt
    12061 tataaataac ttagcaatat aaagaaaaaa acgcacgaac aacataggaa tataaacaaa
    12121 cgtttttgtc acaacgtcta agatcgtgtt aaaaaatagt tatctaaaaa gcagaaagat
    12181 actcaaaata aaaaataaaa aaatgaataa aaacgttttc aaatacaagg taatttgtta
    12241 ttataaaata aagaaaaaac aaaataaaaa taaagaaaag agggaatgaa atggtaaaag
    12301 cagaagtagg aagatcttat atatgcaaac cgatcggagt atctcatgaa ctaatcggct
    12361 gtattgaacg tacatatatc aacacggctg tcgtgtatgt tgaaagttat gaccaacgag
    12421 actatcaact tatcaatgaa agcaagtatc gcatgctcgt aaaattctgt gatattagcg
    12481 cacctactga attggtttct taaacagtat ttttgagaat tcgcaagaac tcccttataa
    12541 tataaataaa gacagaaggg agcgagaaga tatgccaaaa agaaaaagtt cagggaaaat
    12601 gggaattatt cttgcagtga tctctgcaat tcctgctatt ttagctattg ttcgtgcgat
    12661 gaaggaatca agagaaagtt aagcacacat tatgagcgtc aaacaaaaaa cgcatatacc
    12721 tgcggtggca ggttatgcgt tttttgttgt ataaagactt ttttcattaa cgatttttga
    12781 ttagccgact cccaaaacaa aatttagcag tttatctgaa taagtgattt cttcatctgt
    12841 ttgtttcaca ctttcattta gtagtcccat ctcatcggtt atgataccat ccacaccata
    12901 aaacatcatg cgactcatgg catctgaatc gttgattgtc catacatata cttcttttcc
    12961 gtcttggtgg gcagaattaa tgaagttacg gttgatcgta ctatattcca ttgtcagaaa
    13021 atcagcaggc gtcacagggg gcccaacaat attgaaaggt aagatgtagc ccacataaaa
    13081 gtttggttct gcttctttta aatctgaaac ggtttggtac gtcaaacttt gcaaaatatg
    13141 atggttctct aagataactt tgcggtattt atggacaaat cgttcgacaa ggtcatccga
    13201 atctttttta gaggtcttga tctcaataag taatttttgg tttagcttat gtgctttttc
    13261 caaataagaa tcaaaagaac aaacaggggc ttgcactccg ttttcagtaa ctgtaagatt
    13321 tgtcaattct ttcaatgtaa gatcctgtgg tgttccattt acccctgtta agtgacgaag
    13381 atttgcatca tgcatcacta cgaaatgacc atcttttgtt tcctgaacat ccatctctac
    13441 ataatcagga tgatagtctc tgcttgtttt ttcaagtgct tctaaagtat tttgcgcccc
    13501 attttgggta ctcacacctc tgtgagagac agtcactggt tttgtctgaa cagctgaagt
    13561 cagataatcc atattgtaaa gacagacgcc aataccgaaa aaaactgcaa aaagagtcaa
    13621 accagtgttt ttcagcgcag agaaacgcaa gttgggggct tgtgggataa accacttagg
    13681 gatctcaggc aagaagcctt cgtcatccat gaaatcaaca atgatataaa aaatccctac
    13741 ggtagacatc acgatattga ataataaaat cccttgtaaa agcgtcatag caaacacagc
    13801 gctgatcaat gcatagtctg ggaaaaattg ttcaaccata ctttgtgcca aaataacaac
    13861 gatatatcct aaagaagaaa ggagtaaaat cgtaccgcca atcacaataa attggcccgt
    13921 gatggccaaa agacgcgatt tggtcaatga ccagctttcc cgaatggctg ctcgaaaaga
    13981 acgatctcgt agaatcattt ccggtaaagc aaaaatcagt cgaatcccaa tatagccaag
    14041 gaaaatataa acgagtaaaa aggaactgac gatgatccaa cgattagcaa aaataaaatc
    14101 cataatgaaa gcggggattt ttatttttga cagtagatca gaattaaaac ttaatccgct
    14161 gatcggtaga atcaataaaa aataagcaag gaaaaacaaa aaagtaatgg gtctcacttt
    14221 cttcagctga agtatcgtca agtgtaagag ttgcttcaaa gagattggtt ctttcttttt
    14281 aataaaaaaa acactcatca aaagaaaagt aaattcgaaa taaactaaca gtaaaatcgt
    14341 catcaaaact acgattaacg agagtagaac cccaggatgt tgagaaaaaa ttgaaggaat
    14401 attgtcataa gacaaatagt caattgcccc tcggtgtaaa ataaaacggg tcgtactgct
    14461 caataagggt aaacaaataa ataaaataaa gccatgcatc agcaagacat cacggaaata
    14521 agcttgtgtt ccttttaaaa agtcccaagc attacctaag ctacgtttca aataattcat
    14581 cctatcacct gctcttcaca ttgtagcgca aatctaatga aaaaggaaag ggtgcagtgt
    14641 gttttgcaga ccctttccca gattta
//
LOCUS       DACGLW010000074        14523 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.077, whole genome shotgun sequence.
ACCESSION   DACGLW010000074 DACGLW010000000
VERSION     DACGLW010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14523
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.077"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            <1..197
                     /locus_tag="IXT53_002210"
     CDS             <1..197
                     /locus_tag="IXT53_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906007.1"
                     /translation="WFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFLIIAIIAVGLV
                     FNAAGVKDILLELFNRIIGA"
     gene            314..811
                     /locus_tag="IXT53_002211"
     CDS             314..811
                     /locus_tag="IXT53_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAQ4906008.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSISELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMSDVARYYIEETGALGEVPASLQNYIDYESYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            898..1290
                     /locus_tag="IXT53_002212"
     CDS             898..1290
                     /locus_tag="IXT53_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ4906009.1"
                     /translation="MKKIRSYTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAV
                     MILGNLPPLSMIEGAFLKYFGIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRRKLTY
                     AGKKVTLGRNQPQEAITAVRSEFYGISN"
     gene            1274..3721
                     /locus_tag="IXT53_002213"
     CDS             1274..3721
                     /locus_tag="IXT53_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ4906010.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSILSAQERSKNEVTGKLKTVAYDKIDQQTDALISM
                     IGENQVDYRFFIGFKLLLNDQEFSMKSLTVEAKNAFTDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYPLSKKKLEH
                     ETLIKTYDLIKPTRCLVEEKQRYLKIQQEDETAYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKAERGRWK
                     ETLPEISHEINIVNLTSDEKNKGLLDPYVIMKNTKDSESLAIDILTFLTGISSRDGER
                     FPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTDYDFAHLLFSDGY
                     VEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVISTFALDFIHTDRS
                     IFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDDLLDEKLKNNLGL
                     KFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLYGRVGVIQFHPVF
                     EELLHAFDTRPPVRKEV"
     gene            3724..5907
                     /locus_tag="IXT53_002214"
     CDS             3724..5907
                     /locus_tag="IXT53_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ4906011.1"
                     /translation="MKPSILNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVTPSGLSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHALMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNMTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRM
                     MRKPRMLMHAHMHRLQRKLGRSVTALGAGSAIASATGKKGQSGSGSSASTQADHSRPN
                     GQEKSTLGKRIGQTIGTVADTKDRMVDTAGNLKEQVKDLPTNARYAVYQGKSKVTDNV
                     HDLTRSISQTKADKASGRKKQQEQRRKTIAERRSEMEQVKQKKQPASSVHERPATKQE
                     QSHDGQTSRQTTVQASYRESQQAKQERPTVKSDSSSLKTERQSNTVQERTVQKPVTST
                     APTDRASQRSITKERQSTVQRVPLQNTKSRPPIKTATIKKGSKKP"
     gene            5904..6905
                     /locus_tag="IXT53_002215"
     CDS             5904..6905
                     /locus_tag="IXT53_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAQ4906012.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDGGFSNIHYGGV
                     DVSAEVLAHKPMVEKVAKEYGIEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLGVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGDEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKFEGWKYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTRHIPLLEAKA
                     GDLVFFHSTYNAGSYITHIGIYLGDNRMFHAGDPIGYADLTSSYWQQHLVGAGRIKQ"
     gene            6920..7834
                     /locus_tag="IXT53_002216"
     CDS             6920..7834
                     /locus_tag="IXT53_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ4906013.1"
                     /translation="MMKFGKNQNTEKQTPKEKKPRVYKVNPRKKVVIVLWVLLGLSFS
                     FAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWELNDKSIDNRIEN
                     LKAYLTEELQALNVDTVRKDIPVSSSVKGFQIWTVEADGDNQFDVTYSVDQLITEGEN
                     TKTVHSAYIVSVYVDSTGNMVLIKNPTITSIPKKSDYKPKAIESEGTVDSITTNEINE
                     FLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIYNRKDNQVTVSLTVEY
                     IDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            8079..8195
                     /locus_tag="IXT53_002217"
     CDS             8079..8195
                     /locus_tag="IXT53_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAQ4906014.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            8211..10130
                     /gene="tet(M)"
                     /locus_tag="IXT53_002218"
     CDS             8211..10130
                     /gene="tet(M)"
                     /locus_tag="IXT53_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAQ4906015.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            10228..11601
                     /gene="tet(L)"
                     /locus_tag="IXT53_002219"
     CDS             10228..11601
                     /gene="tet(L)"
                     /locus_tag="IXT53_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HAQ4906016.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIKKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
     gene            12165..13427
                     /locus_tag="IXT53_002220"
     CDS             12165..13427
                     /locus_tag="IXT53_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plasmid recombination enzyme type 2"
                     /protein_id="HAQ4906017.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYS
                     DKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTA
                     ARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEEN
                     KSLKSKISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHD
                     LEPKKNEFELTHNREVKKERSRDQGMSL"
     gene            13672..>14523
                     /locus_tag="IXT53_002221"
     CDS             13672..>14523
                     /locus_tag="IXT53_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HAQ4906018.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHI
                     LEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKV
                     VAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQRFRRMMQYKKINKNLVGFMR
                     ATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPNVKV
                     QMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLISYG
                     GLLKEIHK"
BASE COUNT         4741 a         2464 c         3047 g         4271 t
ORIGIN      
        1 actggtttct atcaaacgct caatcactgg tgcttttagc aatcgttgtg attggcttgt
       61 atcttggctt caagcgtgag tttagcaaac tgattggctt tttaattatt gcgattattg
      121 cggttggctt agtcttcaac gctgctggag taaaagacat tttactagag ctattcaatc
      181 gcattattgg tgcttaaata aaaccgttct tttgtggaat ataagtggtt ttcttatgtt
      241 ccgcaaagga atggtacacc aaacgaagtg cggtagggat ttttgaatct ctacaaagaa
      301 aggacgtgaa tatatggacg atatgcaagt ctatattgcg aatttaggca aatacaatga
      361 gggcgaattg gtcggtgcgt ggtttacctt tcccattgac tttgaggaag tcaaagagaa
      421 aatcggcttg aatgatgaat atgaggaata cgccattcat gattacgagt taccctttac
      481 ggttgacgaa tacacttcta ttagcgaact caatcgccta tgggaaatgg tatcggagtt
      541 gccagaagaa ctacaatcgg agctatctgc tctgctcact catttttcaa gtattgaaga
      601 actaagcgaa catcaagaag atattatcat tcattccgat tgtgatgata tgtctgacgt
      661 ggcacgctac tacattgaag aaacgggtgc tttaggcgaa gtgccagcca gtcttcaaaa
      721 ctatattgat tatgaatcct atggtcggga tttagacctt tcgggaacgt ttatctcaac
      781 caatcacggg atttttgaaa tcgtctatta aatctgtcgg tacattctta ctggcagatt
      841 ttcttgttta cggggtagtc aaaagactat ccccattttt atgaaaggat tgattacatg
      901 aagaaaatac gaagctatac cagtatctgg tctgttgaga aagtgttgta ctccatcaat
      961 gactttagac ttccgtttcc cataaccttt acgcaaatga catggtttgt cgtgtcactc
     1021 tttgcagtta tgatacttgg caacttgccc cctctttcta tgatagaggg agcatttctc
     1081 aaatactttg ggattcctgt ggctttcaca tggtttatgt ctacaaaaac ctttgatggt
     1141 aaaaagcctt atggattttt gaagtctgtc attgcttatg cactgcgacg aaagctgacc
     1201 tatgcaggaa aaaaagtaac gcttggcaga aaccagccac aagaagccat tacagcagtt
     1261 aggagtgaat tttatggcat atccaattaa atatattgaa aacaatctgg tctggaataa
     1321 agatggggaa tgttacgctt actatgagct tgttccctac aattactcat ttctaagtcc
     1381 ggaacagaaa atacaagtac atgattcttt cagacagctt atcgcacaaa atcgtgatgg
     1441 caagattcat gctttacaaa tcagtacaga atccagcata ctttctgcac aagaacgttc
     1501 caaaaatgaa gtcactggaa agctcaaaac ggttgcctat gacaaaatcg accaacagac
     1561 agacgcttta atatccatga ttggcgaaaa tcaagtggac taccgtttct ttatcggttt
     1621 taagttgctt ctcaacgatc aggagttttc tatgaaaagt cttaccgttg aagcaaaaaa
     1681 tgcttttact gattttgtct atgatgtgaa ccataagctg atgggcgatt ttgttagtat
     1741 gagtaatgat gaaatcctgc gttttcagaa gatggaaaag cttttagaaa ataaaatctc
     1801 ccgtcgtttc aaaatccgaa gattagataa ggatgacttc ggctatctga ttgaacacct
     1861 ttacggacag acaggcactg cctatgaaga gtatgagtat cctctatcaa agaaaaagct
     1921 ggaacatgaa acactgatta aaacctatga cctcattaag cctactcgct gtctggtgga
     1981 agaaaaacag cgatatttga aaatccagca ggaagacgaa accgcctatg tagcttactt
     2041 taccatcaac agtattgtcg gcgaactgga cttcccgtcc tctgaaatct tctactacca
     2101 gcaacagcaa tttacctttc cgattgatac gtcaatgaat gtggaaattg tagcgaatcg
     2161 taaagcccta tctactgtcc gcaataaaaa gaaagaactg aaagacttgg ataaccacgc
     2221 ttggcaaagt gataatgaaa ccagctctaa tgtggcggaa gctctggaaa gtgtgaacga
     2281 gctggaaacc aatttagacc aaagcaagga atctatgtat aagctgtcct atgttgtaag
     2341 ggtatcagca aatgaccttg acgaacttaa acgtcgttgt aatgaagtga aagatttcta
     2401 tgatgatttg agcgtgaaac tggtacgacc ttttggggat atgctgggct tacatgaaga
     2461 atttttacct gccagcaaaa gatatatgaa tgactatatt caatacgtga cctctgattt
     2521 cctcgctggt ttaggttttg gtgctactca aatgctgggt gaaaatgagg ggatttatgt
     2581 tggctacagc ttagatactg gacgcaatgt ctatctgaaa cctgctcttg ccagtcaagg
     2641 ggttaagggt tcagtaacca atgcgttagc gtctgccttt gtcggttcgc tgggtggtgg
     2701 taaatccttt gcgaataacc ttatcgtcta ttatgcagtg ctttatgggg cacaagcagt
     2761 gattgtagac ccaaaagcag aacgtggcag atggaaagaa accttgccag agatttccca
     2821 tgaaatcaat atcgtcaatc tgacttctga tgagaaaaac aaaggcttac ttgaccctta
     2881 tgtaattatg aaaaatacca aagattctga atcactggct attgatattt tgacattcct
     2941 tacggggatt tcctctcgtg atggggaacg cttcccaatc cttagaaaag ccattcgtgc
     3001 agtaaccaat agtgaagtgc gagggttgat gaaagtgatt gaagaattac gggttgagaa
     3061 tacgccacta agtaccagca tagccgacca tatcgaaagt tttacagact atgactttgc
     3121 ccatctgctt tttagtgatg gttatgtaga gcagtctatc agccttgaaa aacaactgaa
     3181 cattatacag gttgccgact tggtacttcc tgacaaggaa acttcctttg aggaatatac
     3241 cacaatggag ttactatcgg tagcaatgct gattgtcatt agtacctttg cgttggactt
     3301 tatccataca gaccgaagta ttttcaagat tgtagactta gacgaagcat ggagcttctt
     3361 acaggtggca caagggaaaa cactatctat gaagctggtt cgtgctggtc gtgctatgaa
     3421 cgctggggta tatttcgtga cccaaaatac agatgacctc ttagatgaaa aactgaaaaa
     3481 taacctcggc ttaaaatttg cctttcgttc cactgacctt aacgagatta aaaaaaccct
     3541 tgcctttttt ggtgtagacc cagaggacga aaacaatcag aagcgattgc gtgatttgga
     3601 aaacgggcaa tgccttatca gtgatttata tggtcgtgtc ggtgtgatac agttccaccc
     3661 tgtatttgaa gaactgctcc atgcctttga taccagacca cctgtgcgaa aagaggtgta
     3721 aatgtgaaac catcaatatt aaacagaata aaatcaaact ggacgctgaa acgtctaggt
     3781 aaagtggcaa tgacagtggc tttcacactt gtgattgcca tttttctttt agccatgctg
     3841 ggaacggtgg ttcaagctgc gggcttggta gatgatacgg tcaatgtggc aaatgaatac
     3901 agccgatacc cacttgaaaa ctatcaactg gatttttatg tggataatag ctggggctgg
     3961 ctaccgtgga actggtcgga cggaattgga aaacaagtca tgtatggact atatgccatt
     4021 accaatttta tttggacaat cagtctttat gtttccaatg cgacgggtta cttagtacag
     4081 gaagcctatt ccttagactt catttccgct acagcagatt ccattggcaa gaatatgcag
     4141 acccttgcag gggttacgcc tagtggacta tcaacagagg gtttctatgt tggattcctt
     4201 ttactcttga ttttggttct tggggtttat gttgcctata caggactgat aaagagagaa
     4261 accacaaagg caattcatgc cctcatgaat tttgtgctgg tttttatcct atcggcttcc
     4321 tttattgcct acgctcccga ctacattaaa aaaatcaatg acttttcatc agacatcagc
     4381 aacgctagtt tgtcactggg tacaaaaatt gtcatgcccc attcagatag tcaaggcaag
     4441 gacagtgtgg acttgatacg tgacagccta ttttccatac aggttcagca accgtggcta
     4501 ctgcttcaat ataacagttc agacattgaa agtattggta ttgaccgtgt ggaaagcctg
     4561 ctctcaacca gtccagattc caacaatggg gaagacagag aaaaaattgt tgcggaagaa
     4621 attgaagata gaagcaatac caatatgacg attacaaaga cgattaaccg tttaggtaca
     4681 gtcttcttcc tatttgtctt caatattggg atttccatat ttgtattcct attaacagga
     4741 atcatgattt tctcgcaggt actttttatc atctatgcta tgtttctgcc tgtgagcttt
     4801 attttaagca tgattccatc atttgatggt atgtcaaaac gagccataac aaagctcttt
     4861 aataccattt tgacacgagc tggaatcaca ttgattatta cgacagcatt tagtatttca
     4921 accatgctct ataccttatc ggctggttat ccgttctttt tgattgcttt tctacagatt
     4981 gtgacctttg caggaatcta ctttaagctg ggcgatttaa tgagtatgtt ttctctacag
     5041 agtaacgatt ctcaaagtgt gggaagtcgt atgatgagaa aacctcgtat gcttatgcac
     5101 gctcacatgc accgtctaca gcggaaactt ggacgctccg tgactgcttt aggggctggg
     5161 tctgccattg ccagtgctac gggtaaaaaa ggacagtcgg gttcgggaag ttctgcgagt
     5221 acacaagcag atcactctcg accaaacggg caggaaaaat caacactagg aaagcgtatc
     5281 ggtcaaacca tcggtacagt agctgatacc aaagacagaa tggtagatac tgctggtaat
     5341 ttgaaagaac aggttaagga tttgccgacc aatgcaagat atgcagtata tcaaggaaaa
     5401 tctaaagtga cggataatgt ccatgattta accagaagta tttctcaaac caaagcggat
     5461 aaagccagtg gacgcaagaa acagcaggaa caaagacgga aaaccattgc ggagcgtcgc
     5521 tctgaaatgg aacaggtcaa acagaaaaaa cagccagctt cttccgttca tgaaagacca
     5581 gccactaagc aagaacaatc tcatgatgga cagacttcaa ggcaaacaac tgtacaggct
     5641 tcctataggg aatctcaaca agccaaacaa gagcgtccaa cagttaagtc cgattcttca
     5701 agtttaaaaa cggaacgtca aagtaataca gttcaagaaa gaacggttca aaagccagta
     5761 acttcaactg caccaacaga tagagcttca caacgttcaa tcacaaaaga acgtcagtct
     5821 actgttcaaa gagtaccact acaaaataca aaaagtagac caccaatcaa aaccgccacc
     5881 attaagaaag gcagtaagaa accatgaagc tgaaaacttt agtgattggt ggttcgggat
     5941 tattcttgat ggtcttctca ctgcttctgt ttgtcgccat tttattttca gatgaacaag
     6001 atggcggttt ttccaacatt cactatggcg gtgtggatgt ttccgcagaa gtgctggctc
     6061 ataagcctat ggtagaaaaa gttgccaaag aatatggaat tgaagaatac gtcaatatac
     6121 ttcttgcgat tatacaggtg gaatcgggtg gtacagcaga agatgttatg cagtcctcgg
     6181 aatccctcgg tcttccacct aattcattga gtacagaaga atccattaag caaggtgtga
     6241 agtatttcag tgagttatta gccagtagcg aaaggcttgg tgtagattta gaatcggtta
     6301 tccagtccta caattatggt ggtggtttct taggatatgt ggctaatcgt gggaataaat
     6361 atacctttga actggctcaa agtttctcaa aagagtattc gggtgacgaa aaagtgtctt
     6421 accccaatcc catagccata cctatcaatg ggggctggcg atacaattac gggaatatgt
     6481 tttatgtgca actggtaacg cagtatcttg tcacaacaga gtttgatgat gatacagtac
     6541 aagccatcat ggacgaagct ctgaaatttg aaggctggaa gtatgtctat ggcggagctt
     6601 ccccgactac ctcttttgat tgtagcggac tgacacaatg gacgtatgga aaagctggaa
     6661 tcaacttacc acgaacagca caacagcaat atgatgtaac ccggcatatc ccattattgg
     6721 aagcaaaagc cggcgatttg gttttctttc attctaccta taacgctggc tcttatatta
     6781 ctcatatcgg gatttattta ggcgataacc gtatgtttca tgcaggcgac cctatcggtt
     6841 atgccgactt aacaagctct tactggcaac agcatttagt gggagcagga cgaatcaaac
     6901 aataagaaag gacgattcaa tgatgaaatt cggaaaaaat cagaatacag aaaaacagac
     6961 accaaaggaa aagaaacctc gtgtctataa ggtcaatcct cgtaaaaagg ttgtgattgt
     7021 cctatgggta cttttaggac ttagtttcag ctttgcgata ttcaagcact ttacagccat
     7081 tgatacccat accattcatg aaaccaccat catagaaaag gaatatgtcg atactcatca
     7141 tgtggaaaat tttgtagaga actttgcgaa agtctactat tcatgggaac taaacgacaa
     7201 gtccattgat aatcgcatag aaaacctgaa agcatatctg acagaggaac ttcaagctct
     7261 gaatgtcgat acagttcgta aagatattcc tgtatcgtct tctgtaaaag gatttcagat
     7321 atggacggta gaagcagatg gcgacaatca atttgatgta acctacagtg tagaccaact
     7381 cattacagag ggggaaaata caaagaccgt ccactctgct tatatagtga gtgtctatgt
     7441 agacagtact ggaaatatgg tcctcattaa gaatccgacc attactagca taccaaagaa
     7501 atcagactat aaaccaaaag ctattgaaag tgagggtacg gttgattcca ttacaaccaa
     7561 tgaaatcaat gagtttttaa cgacgttctt caagctctat cctacagcga cagccagtga
     7621 actttcctac tatgtgaatg acgggatatt aaaaccaatc ggaaaagagt acatctttca
     7681 agaactggta aatcctattt acaatcgtaa ggataatcaa gtcacggtat cgctgacagt
     7741 ggagtatatc gaccagcaga ccaaagcaac gcaggtatct caatttgatt tggtacttga
     7801 aaagaacggg agtaattgga agattataga ataacaaata ttggtacatt attacagcta
     7861 ttttgtaatc acgtactctc tttgataaaa aattggagat tcctttacaa atatgctctt
     7921 acgtgctatt atttaagtat ctatttaaaa ggagttaata aatatgcggc aaggtattct
     7981 taaataaact gtcaatttga tagtgggaac aaataattgg atgtcctttt ttaggagggc
     8041 ttagtttttt gcacccagtt taagaatacc tttatcatgt gattctaaag tatccggaga
     8101 atatctgtat gctttgtatg cctatggtta tgcataaaaa tcccagtgat aagagtattt
     8161 atcactggga tttttatgcc cttttgggct tttgaatgga ggaaaatcac atgaaaatta
     8221 ttaatattgg agttttagct catgttgatg cgggaaaaac taccttaaca gaaagcttat
     8281 tatataacag tggagcgatt acagaattag gaagcgtgga caaaggtaca acgaggacgg
     8341 ataatacgct tttagaacgt cagagaggaa ttacaattca gacaggaata acctcttttc
     8401 agtgggaaaa tacgaaggtg aacatcatag acacgccagg acatatggat ttcttagcag
     8461 aagtatatcg ttcattatca gttttagatg gggcaattct actgatttct gcaaaagatg
     8521 gcgtacaagc acaaactcgt atattatttc atgcacttag gaaaatgggg attcccacaa
     8581 tcttttttat caataagatt gaccaaaatg gaattgattt atcaacggtt tatcaggata
     8641 ttaaagagaa actttctgcc gaaattgtaa tcaaacagaa ggtagaactg tatcctaata
     8701 tgtgtgtgac gaactttacc gaatctgaac aatgggatac ggtaatagag ggaaacgatg
     8761 accttttaga gaaatatatg tccggtaaat cattagaagc attggaactc gaacaagagg
     8821 aaagcataag atttcagaat tgctccttgt accctgttta tcatggaagc gcaaaaagca
     8881 acatagggat tgagcagctt atagaagtga taacgaataa attttattca tcaacataca
     8941 gaaagaagtc tgaactttgc ggaaatgtct tcaaaattga atattcggaa gaaagacaac
     9001 gtcttgcata tgtacgcctt tatggcggaa tcctgcattt gcgggattcg gttagaatat
     9061 cggaaaagga aaaaataaaa attacagaaa tgtatacttc aataaatggt gaattatgta
     9121 aaattgataa ggcttattcc ggggaaattg ttattttgca aaatgagttt ttgaagctaa
     9181 atagtgttct tggagataca aagctattgc cacagagaga gagaattgaa aatccgctcc
     9241 ctctgctgca aacaactgtt gaaccgagca aacctcaaca aagggaaatg ttacttgatg
     9301 cacttttaga aatctccgac agtgacccgc ttctacaata ttatgtggat tctacgacac
     9361 atgaaattat actttctttc ttagggaaag tacaaatgga agtgattagt gcactgttgc
     9421 aagaaaagta tcatgtggag atagaactaa aagagcctac agtcatttat atggagagac
     9481 cgttaaaaaa tgcagaatat accattcaca tcgaagtgcc gccaaatcct ttctgggctt
     9541 ccattggttt atctgtatca ccgcttccgt tgggaagtgg aatgcagtat gagagctcgg
     9601 tttctcttgg atacttaaat caatcatttc aaaatgcagt tatggaaggg atacgctatg
     9661 gttgcgaaca aggattatat ggttggaatg tgacggattg taaaatctgt tttaagtatg
     9721 gcttatacta tagccctgtt agtaccccag cagattttcg gatgcttgct cctattgtat
     9781 tggaacaagt cttaaaaaaa gctggaacag aattgttaga gccatatctt agttttaaaa
     9841 tttatgcgcc acaggaatat ctttcacgag catacaacga tgctcctaaa tattgtgcga
     9901 acatcgtaga cactcaattg aaaaataatg aggtcattct tagtggagaa atccctgctc
     9961 ggtgtattca agaatatcgt agtgatttaa ctttctttac aaatggacgt agtgtttgtt
    10021 taacagagtt aaaagggtac catgttacta ccggtgaacc tgtttgccag ccccgtcgtc
    10081 caaatagtcg gatagataaa gtacgatata tgttcaataa aataacttag tgtattttat
    10141 gttgttatat aaatatggtt tcttgttaaa taagatgaaa tattttttaa taaagatttg
    10201 aattaaagtg taaaggagga attaattatg aagtgtaatg aatgtaacag ggttaaatac
    10261 aaccaaattt taatttggct ttgcgtttta tcttttttta gcgtattaaa tgaaatggtt
    10321 ttgaacgtct cattacctga tattgcaaat gattttaata aaccacctgc gagtacaaac
    10381 tgggtgaaca cagcctttat gttaaccttt tccattggaa cagctgtata tggaaagcta
    10441 tctgatcaat taggcatcaa aaggttactc ctatttggaa ttataataaa ttgtttcggg
    10501 tcggtaattg ggtttgttgg ccattctttc ttttccttac ttattatggc tcgttttatt
    10561 caaggggctg gtgcagctgc atttccagca ctcgtaatgg ttgtagttgc gcgctatatt
    10621 ccaaaggaaa ataggggtaa agcatttggt cttattggat cgatagtagc catgggagaa
    10681 ggagtcggtc cagcgattgg tggaatgata gcccattata ttcattggtc ctatcttcta
    10741 ctcattccta tgataacaat tatcactgtt ccgtttctta tgaaattatt aaagaaagaa
    10801 gtaaggataa aaggtcattt tgatatcaaa ggaattatac taatgtctgt aggcattgta
    10861 ttttttatgt tgtttacaac atcatatagc atttcttttc ttatcgttag cgtgctgtca
    10921 ttcctgatat ttgtaaaaca tatcaagaaa gtaacagatc cttttgttga tcccggatta
    10981 gggaaaaata taccttttat gattggagtt ctttgtgggg gaattatatt tggaacagta
    11041 gcagggtttg tctctatggt tccttatatg atgaaagatg ttcaccagct aagtactgcc
    11101 gaaatcggaa gtgtaattat tttccctgga acaatgagtg tcattatttt cggctacatt
    11161 ggtgggatac ttgttgatag aagaggtcct ttatacgtgt taaacatcgg agttacattt
    11221 ctttctgtta gctttttaac tgcttccttt cttttagaaa caacatcatg gttcatgaca
    11281 attataatcg tatttgtttt aggtgggctt tcgttcacca aaacagttat atcaacaatt
    11341 gtttcaagta gcttgaaaca gcaggaagct ggtgctggaa tgagtttgct taactttacc
    11401 agctttttat cagagggaac aggtattgca attgtaggtg gtttattatc cataccctta
    11461 cttgatcaaa ggttgttacc tatggaagtt gatcagtcaa cttatctgta tagtaatttg
    11521 ttattacttt tttcaggaat cattgtcatt agttggctgg ttaccttgaa tgtatataaa
    11581 cattctcaaa gggatttcta aatcgttaag ggatcaactt tgggagagag ttcaaaattg
    11641 atcctttttt tataacagga attcaaatct ttttgttcca ttaaagggcg cgattgctga
    11701 ataaaagata cgagagacct ctcttgtatc ttttttattt tgagtggttt tgtccgttac
    11761 actagaaaac cgaaagacaa taaaaatttt attcttgctg agtctggctt tcggtaagct
    11821 agacaaaacg gacaaaataa aaattggcaa gggtttaaag gtggagattt tttgagtgat
    11881 cttctcaaaa aatactacct gtcccttgct gatttttaaa cgagcacgag agcaaaaccc
    11941 ccctttgctg aggtggcaga gggcaggttt ttttgtttct tttttctcgt aaaaaaaaga
    12001 aaggtcttaa aggttttatg gttttggtcg gcactgccga cagcctcgca gagcacacac
    12061 tttatgaata taaagtatag tgtgttatac tttacttgga agtggttgcc ggaaagagcg
    12121 aaaatgcctc acatttgtgc cacctaaaaa ggagcgattt acatatgagt tatgcagttt
    12181 gtagaatgca aaaagtgaaa tcagctggac taaaaggcat gcaatttcat aatcaaagag
    12241 agcgaaaaag tagaacgaat gatgatattg accatgagcg aacacgtgaa aattatgatt
    12301 tgaaaaatga taaaaatatt gattacaacg aacgtgtcaa agaaattatt gaatcacaaa
    12361 aaacaggtac aagaaaaacg aggaaagatg ctgttcttgt aaatgagttg ctagtaacat
    12421 ctgaccgaga tttttttgag caactggatc caggagaaca aaaacgattt tttgaggaaa
    12481 gttataaatt attttccgaa cgatatggca agcaaaatat tgcttatgca acagttcata
    12541 atgatgagca aacccctcac atgcatttag gtgttgtgcc tatgcgtgat ggaaaactgc
    12601 aaggaaaaaa tgtgtttaat cgtcaagaac tgttatggct acaagataaa ttccccgagc
    12661 atatgaaaaa acagggtttt gagttgaagc gtggtgaacg tggctctgac cgtaaacata
    12721 ttgagacagc taaatttaaa aaacaaactt tggaaaaaga gattgatttt ctagaaaaaa
    12781 atttagcagt taaaaaagat gaatggactg cttatagcga taaagttaaa tcagatttag
    12841 aagtaccagc gaaacgacac atgaaaagtg ttgaagtgcc aacgggtgaa aagtccatgt
    12901 ttggtttggg aaaagaaata atgaaaacag aaaagaaacc aaccaaaaat gttgttatat
    12961 cggagcgtga ttataaaaac ttagtgactg ctgcgagaga taacgatagg ttaaaacagc
    13021 atgttagaaa tctcatgagt actgatatgg cgagagaata taaaaaatta agtaaagaac
    13081 atgggcaagt taaagaaaaa tatagtggtc ttgtagagcg atttaatgaa aatgtaaatg
    13141 attataatga gttgcttgaa gaaaacaagt ctttaaagtc taaaataagc gatttaaagc
    13201 gtgatgtgag tttaatctat gaaagcacta aggaattcct taaggaacgt acagacggct
    13261 taaaagcctt taaaaacgtt tttaaggggt ttgtagacaa ggtaaaggat aaaacagcac
    13321 aattccaaga aaaacacgat ttagaaccta aaaagaacga atttgaacta actcataacc
    13381 gagaggtaaa aaaagaacga agtcgagatc agggaatgag tttataaaat aaaaaaagca
    13441 cctgaaaagg tgtctttttt tgatggtttt gaacttgttc tttcttatct tgatatatat
    13501 agaaataacg tcatttttat tttagttgct gaaaggtgcg ttgaagtgtt ggtatgtatg
    13561 tgttttaaag tattgaaaac ccttaaaatt ggttgcacag aaaaacccca tctgttaaag
    13621 ttataagtga ccaaacaaat aactaaatag atgggggttt cttttaatat tatgtgtcct
    13681 aatagtagca tttattcaga tgaaaaatca agggttttag tggacaagac aaaaagtgga
    13741 aaagtgagac catggagaga aaagaaaatc gctaatgttg attactttga acttctgcat
    13801 attcttgaat ttaaaaaggc tgaaagagta aaagattgtg ctgaaatatt agagtataaa
    13861 caaaatcgtg aaacaggcga aagaaagttg tatcgagtgt ggttttgtaa atccaggctt
    13921 tgtccaatgt gcaactggag gagagcaatg aaacatggca ttcagtcaca aaaggttgtt
    13981 gctgaagtta ttaaacaaaa gccaacagtt cgttggttgt ttctcacatt aacagttaaa
    14041 aatgtttatg atggcgaaga attaaataag agtttgtcag atatggctca aagatttcgc
    14101 cgaatgatgc aatataaaaa aattaataaa aatcttgttg gttttatgcg tgcaacggaa
    14161 gtgacaataa ataataaaga taattcttat aatcagcaca tgcatgtatt ggtatgtgtg
    14221 gaaccaactt attttaagaa tacagaaaac tacgtgaatc aaaaacaatg gattcaattt
    14281 tggaaaaagg caatgaaatt agactatgat ccaaatgtaa aagttcaaat gattcgaccg
    14341 aaaaataaat ataaatcgga tatacaatcg gcaattgacg aaactgcaaa atatcctgta
    14401 aaggatacgg attttatgac cgatgatgaa gaaaagaatt tgaaacgttt gtctgatttg
    14461 gaggaaggtt tacaccgtaa aaggttaatc tcctatggtg gtttgttaaa agaaatacat
    14521 aaa
//
LOCUS       DACGLW010000075        14440 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.078, whole genome shotgun sequence.
ACCESSION   DACGLW010000075 DACGLW010000000
VERSION     DACGLW010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.078"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            complement(58..855)
                     /locus_tag="IXT53_002222"
     CDS             complement(58..855)
                     /locus_tag="IXT53_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HAQ4906019.1"
                     /translation="MKVSQKIILSILLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEP
                     KSVPTTLNVALIGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGM
                     NKINAAYSYGGVDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSL
                     DLDGVYINKGEQVIKAISQQLKDVTSIFKLPKAVGKLLGSIQTNLPESVLLDCGMDFL
                     KDNNKKIDTLSVPVDGSWDFNDNTPSGSVLELDLTKNQEAIKKFLNN"
     gene            complement(947..2110)
                     /locus_tag="IXT53_002223"
     CDS             complement(947..2110)
                     /locus_tag="IXT53_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HAQ4906020.1"
                     /translation="MKKRKKIDWWILGPYLTLSMIGLLEVYSASSYRLLQADENTKSL
                     LLRQLIFIFLSWSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGA
                     QRWISLFGIQFQPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPK
                     IAGALMILSIAWVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNH
                     AYERIATLRDSFIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIF
                     SIITEELGLIGALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVG
                     SIAGLMPMTGVPLPFVSYGGTSYLILSLGIGITLNISSKIQAEELPLYRPEKQ"
     gene            complement(2627..2833)
                     /locus_tag="IXT53_002224"
     CDS             complement(2627..2833)
                     /locus_tag="IXT53_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906021.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            complement(2849..3463)
                     /locus_tag="IXT53_002225"
     CDS             complement(2849..3463)
                     /locus_tag="IXT53_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HAQ4906022.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETKEAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(3610..4293)
                     /locus_tag="IXT53_002226"
     CDS             complement(3610..4293)
                     /locus_tag="IXT53_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAQ4906023.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RSNTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(4402..4860)
                     /locus_tag="IXT53_002227"
     CDS             complement(4402..4860)
                     /locus_tag="IXT53_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HAQ4906024.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(4944..6707)
                     /locus_tag="IXT53_002228"
     CDS             complement(4944..6707)
                     /locus_tag="IXT53_002228"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HAQ4906025.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(6928..8388)
                     /locus_tag="IXT53_002229"
     CDS             complement(6928..8388)
                     /locus_tag="IXT53_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906026.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(8596..9522)
                     /gene="glsA"
                     /locus_tag="IXT53_002230"
     CDS             complement(8596..9522)
                     /gene="glsA"
                     /locus_tag="IXT53_002230"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HAQ4906027.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(9545..10972)
                     /locus_tag="IXT53_002231"
     CDS             complement(9545..10972)
                     /locus_tag="IXT53_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ4906028.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(11003..12217)
                     /locus_tag="IXT53_002232"
     CDS             complement(11003..12217)
                     /locus_tag="IXT53_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HAQ4906029.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            complement(12558..12800)
                     /locus_tag="IXT53_002233"
     CDS             complement(12558..12800)
                     /locus_tag="IXT53_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ4906030.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(12832..13389)
                     /locus_tag="IXT53_002234"
     CDS             complement(12832..13389)
                     /locus_tag="IXT53_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ4906031.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(13402..13590)
                     /locus_tag="IXT53_002235"
     CDS             complement(13402..13590)
                     /locus_tag="IXT53_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAQ4906032.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(13603..14169)
                     /gene="amaP"
                     /locus_tag="IXT53_002236"
     CDS             complement(13603..14169)
                     /gene="amaP"
                     /locus_tag="IXT53_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAQ4906033.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
BASE COUNT         4308 a         2968 c         2365 g         4799 t
ORIGIN      
        1 aaaagaagtc agaacataac atagattgtt atttctaact tcttttattt tctttactta
       61 attattcaga aattttttga ttgcttcttg gtttttggtc aaatctaatt ccagaacact
      121 tccggaaggc gtattgtcgt tgaagtccca actgccgtct actggtacgg ataatgtatc
      181 gatcttttta ttattgtctt tgaggaaatc cataccacaa tccagaagca cactttctgg
      241 aaggttcgtt tggatactac caagtaattt cccaaccgct ttgggtagct tgaatattga
      301 agttacgtct ttcagctgct gagagatagc tttgatcact tgctctcctt tgtttatata
      361 tacgccatct aaatctagag atttttctgc atcgatcttg actccaccag gaaacagttc
      421 attaacgcaa tcgataaaat cttgaaatgt gatacttgca taatagggag cttcaaattt
      481 gaaattttcc tttaatgttt gatttaataa atcaactccc ccataagagt aagctgcatt
      541 tatcttattc attccgtaac ctggaatatc gacatatgaa tctctcatga tagagatcag
      601 ttttgcttgt tgtgttttct ggttgtattg tgcaaccaga agtgaatccg agcgaccgtt
      661 ttcgtctttc gaacgagcat ctgaaccaat caaagccaca tttagtgtgg tagggacact
      721 tttcggctct acctttttca cagttgaact ctgttttgca gacgaaaatc catggataaa
      781 ctcaatcgaa aaatagccaa ctactaatgt aagcagtaac aaaatagata atatgatttt
      841 ttgagatact ttcattgcga attcctcttt ctaaaagaac ctctcgcttg aaaaataaaa
      901 gattgtactt tatgataatt caaactgaga gtttctcagt ttctctttat tgtttttcag
      961 gtcgatagag cggcaactct tcggcctgta tctttgaaga aatattcaac gtgattccga
     1021 ttcctaaaga aaggatcaga taacttgttc ctccgtagct aacaaatggc aatggcacac
     1081 cagtcattgg catcagcccc gcgatacttc ccacgttcat tatcgtctga acgaatagca
     1141 aagttccgaa tcctaaaaga aacaatccag cttgctgatt tttacagcga ctgcttaaac
     1201 aaaagattct catgcataat gagaacaata agaaaagcac acacaatgca ccgattagcc
     1261 caagctcttc tgtaataatc gaaaaaataa aatctgtttc tgtctctgga agataccctt
     1321 tttttgtgat actatttcct aatccccgcc caaatatgcc accattatat agtgcataaa
     1381 acgaatgggt catttgatag cccgcaccat gactatcgat aaaagaatct cttaaagtcg
     1441 cgatcctttc ataagcatga ttaaacatct gtggaagcca acctttattt cctaaatata
     1501 ataccccgcc tgctgctcca atcagcaatg cagaaaaagt aacgattaga tagatccctt
     1561 ttttaaatgg aaccgccgct gcccaaaata tgacccacgc aatcgaaagg atcatcaatg
     1621 ctccagcaat ctttggctga aataaaatca gaaaagttat acctactgta atcaggaatg
     1681 gtttttttag atcttttggg ctactatttc cgtcacgaaa aaaccagctt aaataaaaaa
     1741 tcaaaaaaag atttgccagt tcagaaggct ggaattgaat gccaaacaga gagatccaac
     1801 gttgtgcgcc gttgacagtg acaccgaata tccctattct tactaatact aaaaagaaaa
     1861 tcgataaggc taaaccgtat cctgctattt tagggtgaag taaatagtgt agtttgactg
     1921 aacgagctaa gaagatcacg ctccaactca aaaatatgaa aattagttga cgcaataata
     1981 gactttttgt attttcatcg gcttgcaata aacgataaga actggcactg tatacttcta
     2041 aaagaccgat catggacaaa gtcaaataag gacccaaaat ccaccaatcg atcttttttc
     2101 tttttttcac cgcatctcct cctcacacag caatccactt gtcaggtgag gagcgagttt
     2161 gtctcatcaa ataagtcagt cacaaaaata taaagaaaag gtcagaaacc ttttcgaaca
     2221 agaattctga cctagcatca tctcatcgtc cgagttttag cactatacaa cgtaaagaaa
     2281 acattcttag ctattggaaa aagccttaat ccgacagttt ctgttttacc cattggcgac
     2341 tttcgacgtt tctgggcaac agcctatgtt tgtataggag cctcacctaa caaggttatt
     2401 actatattct atttacgagt tcatgataga cttttcctaa tgattaggca atggaatttg
     2461 aaatgaaata gtgaaattta acaatcttta atatttttta gaaaaaaata acaaataagt
     2521 tgaaagaatc ccgttgcaaa ttctttcagt tgcaccagga ttctttgata attttttagt
     2581 tttatgtgtt tttttatatg tttttttcac ttttcggaat aaatatttat ttgttaaagt
     2641 aattaaacaa tccgctaatc gtttgatctg gcatttctat ttccttacca tctaaagtaa
     2701 cagtagtaac gacaaaatgg tcttgcaatg gcggaacttc taagtactgc ccttgcagaa
     2761 aatcattcac tgtttcatat tcttttttca tttcttttga atctgtcgat ggtttatagc
     2821 ttatttttaa cataactagt gctccttttt attcgggacg tgcaatcgtg aaaatttctc
     2881 ctattgctgc ataatcattt cctgccaaac ggcttacagc attcaatttt cttggatcga
     2941 tccttcctgt cttatcatca tagactgttt catttaaatg atatttctgt acttctagca
     3001 aaaataaatc agctgttact ttatttttat tccgtatctc gacatgttca tataaggatg
     3061 tttcaaaacg aacttttgct tctgtcaatg ctggtactga aatgattact gaatcagtta
     3121 aagtcaagtc agtcaggtac agttcgcttt cctcattagg cagattggct gctgttttat
     3181 ttacttcttc tacgttgtcc tcatcaagga tatggataac agcttctttt gtctcaagaa
     3241 tattctttgc tgtgtctttt actgcatctt gttttctctg gatcgccaag gataagatgg
     3301 gtcgattcga actgacgata ttgaagtaac tgaagggtgc gatattcact atatcttttg
     3361 ctgatttagt cgttactact gctactggtc tagggatgac tgaaccaatc agcaacttgt
     3421 aattttcacg ttctgtcaat tctgaaggac tccgttcaaa catggtttcg ccttctttct
     3481 ataaaattcg ttaaaaagca gatcttgaag acaaaatgaa aagtaatgct tttttcatct
     3541 tcttagaatc tgctttaaac tatctatcaa tttttctttt ttgtcaagaa tctgtgccgt
     3601 gcgtgttttt tagttatttg ctttacttgc ggcttcgaca tatcttttag tgatcaattg
     3661 cggacgggtg ataatagatc caactacgac tgaaaaagca cctaattcat atgctttctc
     3721 tacttgttca ggagagtcag tatgtccttc cataattact gggatatcga cttgttctat
     3781 gatttgattc aataaatcaa aatcaggtgt ctgcgtcttt cgtgaatatt ctgtatagcc
     3841 tgctaaagtc gtagacaaaa gatcaaaacc atcttcttgc gcttgaagcg cttcctcaaa
     3901 agtcgaaata tctgccatag ctaaccgacc gtgttcgtga atcatttgga caagttcagc
     3961 agttgttttt tctgtcggac gtttacgaga agttgcatcc agtgcaatga tttctgcttg
     4021 cgtctccaaa agttcttcca cttctttttc tgtcgcagta ataaacactt ctgacccatc
     4081 gtaatctctt ttgataatcc ctattaccgg taaatcaacg attcgcttga tttcattgat
     4141 atcttctgtc gtatttgatc gaataccaac tgctccgcct tcttttgcag ccaaagccat
     4201 tctccccata ataaaagaac tgtgtagcgg ctcgttttcc aaagcttgac aagaaacgac
     4261 gactccgcct ttgatttgtt ccagtgtatc cacaatatct cctccagtaa tgttatataa
     4321 aaatacagct tcatataaaa atgtaaccct tttctattca ggagtcaata atgagctatt
     4381 tctttcagca gacctttctt actaaaagaa aattacttca aattcttcgt atacgaccag
     4441 cttctcttct gtaaattcta tgttcaacgc ttctttataa gcatgtttaa aaaactcagc
     4501 atggactttc cgccaataag ctaatgtccg atccccttct ccttctttcc aagcatgctc
     4561 tttcgtgact tcactaaaag gcaagatggt cacatttata tttttgatga tacaaactgg
     4621 ttcatttttt ccattcagga ccacgtctaa ttgaccaact tttggtaaag gctcattctc
     4681 ttgtttatac agatcgtaac cacttgatgt accagtcttt atctttttta caactagttc
     4741 gctcaactcg tctgccattt tttctgtatc cccaaatgaa aagaaaccta atttttctga
     4801 ttgataacta ggatattttt cttggaactt tttgacatat tcttctactt gctgatacat
     4861 aacatccacc cctttgcttt ttataatgaa aaaaccatcc agatactagt ataaacaatt
     4921 atctggatgg tgagcataat gaattattga atattcaaga cacgcagact atcttcaacg
     4981 atttgttttg gtgttaaatg ataacgttca taaagctgcc ctacaggaac gctatccgtg
     5041 aattctttct ctgcaccaaa atttaatacg ttcattgaag aatcaccata ataacgactg
     5101 atattttcgc caaatcctcc tgacaaacta ccatcttcta atgtgatgac taattcgtgt
     5161 gtttctttta atttatctaa tgtttctacg tcattttctg tgatcgttcc tggattgatc
     5221 aacgttgctt cgattccttt ttttctaaaa gcttttactg tttcttttcc taattcaaga
     5281 aaaccgccta aagctaaaat tgctactttt cctccattat ttactgtttg ataggctgga
     5341 atactataat catttgcgat agctgtacca tgttctacac catgtgtagg aattcggatt
     5401 gctacaggct gcgctgtttg ttcgaacgcc caatctagca tagagatcat atcctctttt
     5461 gttgctggag caagatattt gatgttcggg atacttgata agtaaggaat gtcaaatgtt
     5521 ccttggtgtg ttcggtcact agctgagatc gttccgccac cgactaaaat aatcactgga
     5581 ttctcgttga tcgctaaatc atgtgataat tgatcatatg cccgttgcaa gaaagtactt
     5641 gattggaaga cgactggacg tactccctga gaagccaaag cagaagcgaa agtgatagag
     5701 tgctgttcag cgatccccac gtcaaaataa tgatctggat gtttttcttg gaatgttttc
     5761 agtccaaagg cgcctggtat cgctgcattg actgcggcaa taggagctgt acttgtttgc
     5821 attttcttat ccaaataatc catgatgatc atattaaatg tttctccaga agttacttgc
     5881 tttgcctccc cggtggacag atcaaatggt acatgccaat gatatttcat tttattatcg
     5941 attgccggct gataaccgcg acctttttcc gtatggacgt gaagtacaat cggatgatca
     6001 atatctttta cttcttcaaa taaacggatc accgtttcca gatcgtttcc attatccacg
     6061 taacgataat caaatcccat tgctttaaat aggttattgc tgcttttacc gttggtttga
     6121 cgaagctcag ctaagttttt atataaactg ccatgattct catcaataga catctcatta
     6181 tcgtttacta atacgattag attggattgt aaaacaccac cattgttcaa tccttcaaaa
     6241 gctaagccac cacttaggga gccgtcaccg ataaatgcta ccacatttcc tgattctttc
     6301 ttcagatctc tcgctttagc taaacctgtt gctaagctga tagatgtaga tgtgtgcccc
     6361 actgtaaaat agtcatgttt actttcatgt tggttggaat atccgctcac ttcacggtaa
     6421 tgtccttttt caaaaccatt ttttcgacca gttaaaagct tatgaccata agactgatgg
     6481 gaaacatccc aaacgatttt atcctttggt gaatcaaata cataatgaaa cgcaatcgtt
     6541 aattctacta cacctagatt cggaccaacg tgcccaccta catcgctgtc cttttgcaag
     6601 attttttggc gcatctcttc tgccaactgt ttcatttcct caacagataa ttttttgaca
     6661 tctgcggggc tattgattgc atccaataca tgtaatactg tactcataat acattctctc
     6721 ctccgtacta tcttctttta atgaaaatag atttgttaca ctttatctat tctactactt
     6781 agagttgact ctaagtcaat agcattttaa aaaaatttca gagaaagctt gttatataag
     6841 tagatcttcg cacgaatcag gactcaaata aaaaccttct acagacaatt aaaaagcttt
     6901 gtagaaggtc tcctaattaa ggtctcatca agaaatatgt ttttttaata ggtgaagttc
     6961 ctgagcaata tgatacaaat ggatgtttgc accttttaat gagattggat agatctttgt
     7021 cttttcagtt tctaatccat tttgatagct agcagcatcg cacaaaacca ctcttttttt
     7081 cgcacataga cattcatcag gcaattcttc taatgaacga aataaaatag gatttatttc
     7141 agcgatgcct tcttccgcat acaaatgaaa ttgggttcga taaaaaaatt gtttgactga
     7201 actttctgaa acaacaaaaa tttctatttc ctgtctcccc gcttgttcac tgttgatcaa
     7261 agaaagactt ttgacgaatt ttcgaatgaa attttcattc catcttagtt ggatttgatt
     7321 tttctttttc cactcttcca atatttctac aatttttccg tacagaagtt gttctttttc
     7381 tgatagaaga tagatccttt ccggtaaaaa tacttgtcca tctgcccaca acgtacggac
     7441 aaattgtaaa aaggattgct tgaaaataaa attttctgca tcgtcgattt cagcgatact
     7501 aataatctgt aaaatcaatt cattcagtga taggtcattg agaacaaaat ttttataaac
     7561 aacttcgaaa tcttttctta acagctccat gttctcattg gtataatttc tactgttgaa
     7621 caaagaaaaa atataataga catcttcttc ttgtaagtct agaattcctt tttgctcttc
     7681 caatccttga cagactaatt gaaataacgg cttcgtttct gcgcttttct tatcttcttc
     7741 tttcagtcca aggggaaatg cctttcttcc gattgcgatc tctattcctc gtatgataaa
     7801 ggacttttca tcttccgaat atcttctttt aaaaaaatga gtctctacat aatcaatcaa
     7861 ctcgttacaa gcttcttcta catacggatt tttattttca tagccttccc aattgacata
     7921 acgtactaaa gctagcaaga tgttccgcga gtcaaattct gggataatga tttctttatt
     7981 tttcaaataa tcattttctt taaaaaaatc caatacgatt tttttgattg tatatacttt
     8041 gctcaaagaa ataaattcaa gatcagccag ttctgtactc gtgaatatac cattaaaata
     8101 ataacttaaa aactttaaaa aataagattg accgtacaga aactgtgtga gctcagaaag
     8161 agcaatacca tgtttgaata cgagtttata cttatgcttc tctcttactt gttcttttat
     8221 acaatattct agacactcgg ctagactttc tagatcgtga aggatcgttg gtgtagtcac
     8281 ccttagagaa atagctgttt gttcgatgtc tatttcttgg aattcaaata attgttcaca
     8341 cagatacact tttctaaata tatttcgctc gatgtaattt tccagcattc ctgatacacg
     8401 cctccagttt ttcttatgat tttatttaat catatctgta aatcgtattt ttgtttcctt
     8461 tttatttaga aaagcccttt caaaaaccaa taaaaaaaga aactgggaca aaagtcaaac
     8521 aagctcacaa tttcagcctc attgtaggta gctttgagcc gtttggactt ttaccccagc
     8581 ctctttcaag aatggttatt caaagatgtc agcgtctagt tctttgacta catctttcaa
     8641 caattggata ccagctgcgc tgttaccaaa tggatctagt gccgggctga agacacctat
     8701 gccgtaacga tttggcacag ctgccatcaa cccgccgcct acaccgcttt ttgcaggtac
     8761 accaacatgg acggaaaact ctcctgattc atcatacaaa ccagcagttg tcataattga
     8821 tttgacgatt gtagcacttt cttcagtaat gatttgtttg ccattccaag gagcaattcc
     8881 tttatttgcc agcacagcag ctaattttgc tatgcctgta gcggtgacat tgagggagca
     8941 ttgtctgaaa taagcatcga gtatctccgg tacgtctcct tcgatcattt gattaccttt
     9001 catatagtaa gcaagagagc gattgatatc acctgttctt gattcagaat gatagatttc
     9061 ttcattcaat gtgatattag gatcatcaca gatctctttc ataaatgaca agattcgctc
     9121 aaatttttca tctgcatctt taccggcaat caacgaggtt gttgcaatcg ctcctgcatt
     9181 aacaaaagga ttcattggat gtttgcgatg attgatttcc atattcatga tagaattgaa
     9241 ggcaaaaccg gttggttccg tgttgattgt ttgaaagact ttttctattc cgttgtcttt
     9301 gattgctaat aacaatacgg gaacttttga catgctttcg atggcaaagc gcacatcggc
     9361 gtctcctccc tcaatcagtt gttttgtatc caaatcataa atcgccatac ctaattgttt
     9421 gggatccact tcagctaatg ctggaatgta atccgccact tgtcctgttt gataatagtg
     9481 gaaatttttt ccaatcaatt gttctaattc ttttacttcc atttgaataa ccagctttca
     9541 ttttttattt ttctgcatgc tggaatttat gacggcatgc gtagatcgta aatggaataa
     9601 ctaagacgat aagataagaa acaatcagaa tcgtttcata aatcccatgc tgtccagctg
     9661 cgatactagc aggtggaaca aatgagatac aaaaagcaaa aagtgaagtc aaaagaccta
     9721 aacaagcaac gattactttt acaacttttc cgcctggaac ctgataggct ctttcaagac
     9781 tgtcatcttt aaaaaccagc ttcaaatagc ccgcgaaaaa cataaagtac aacatcaggt
     9841 agattacaac agtcaatgac atcgctgcaa gaaatgaaag attattcccc ccgccgctta
     9901 atgtaaagac ggctgcccag attgttgcaa cgatcccatt tgcgatcatc aaatgagcag
     9961 gaacaccgtg tgtgttttct tttgcaaaat attcaggaag taatccttct tctgctgcta
    10021 cgtgaagtgc ttttgaagga gaggttaccc aagaagcaat ttctccgatg acacctaaac
    10081 aaacaagaat cgcaagaatt ttcgcaatcc atgcaagatt tggatcaatg tgtcttaata
    10141 ggaattctaa cgattgaatc acccctgtat taagagacaa accatgttga ggtactgtag
    10201 tagcgacggt caatccgcca aaagtatcta aaatgattgc cagaataacc aatacaaaca
    10261 ttgctaatgg ataatccttt ttagggtttt ccatttcatt tgcatgactg gcagatgttt
    10321 ctacgcccat atatgctaaa ataaaggaaa caaaaacaac cagcgtaggc aatttcgaaa
    10381 aatcaggaat caatgttttc atagacagta tcgtttgtag tggagcgcct gaagcgacat
    10441 aatggatccc taagccaaac aagactaatg ctggcaacaa gataccagct acaaaaccga
    10501 tccgagcaat caatgaagtg tatttggttc cttttaactg tgagagtaaa atagcccaga
    10561 aaataatcaa taggattgcc aatttcagat atttattgtt ttgtatagca ccaatacccg
    10621 ttgcatagga taatgcacca gttaagaaat acagcatggt caagtagcca actgttatct
    10681 caaaccattg gaaaaagatt gcggcaaaac cgaatcgttt tcccaatgtg cgactgaccc
    10741 aagtatagac accgcctttt tcccatcctt tcaccgttgc catctctgct gcgcacaatg
    10801 ccacagggat aaaccatagc aaaccgccta ataacaagaa aaatactaag ctgaagcctg
    10861 aggtcgcgaa tgtgggatat tcatataatg aaacgaccat tgagatggtc atagccaaaa
    10921 agccaaagaa cgttaatttt tttgcaggaa ttttcgtttt gctttgttgc atgaatcttc
    10981 ctcttttcta tttgaatttg tttcaattga gtttgttgcc aaagaagaaa aagtccaatc
    11041 cttcttcttg ttcttctttt gtagtagtga tcgttgtaaa gtgcatcgcc gattttaaaa
    11101 tcactgttgt cagcacatag gcatacacgg cagtaatgat gacaccaatc acttgaatcc
    11161 ctaagaagta ccagctgctt ggattcgtta gtaaatggct gttagatgca aagatcccta
    11221 tcagaatcgt acctgtgaat ccgcccatgc catgaacacc ccacacatca agtgcatcat
    11281 cccattgttt ctttttacga aaatcaacag cagcatgaca gacgataccc gcaatgatcc
    11341 caatcagcat ggcacttttt gcttctacat aaccggcaca aggcgtaatg gctgccaatc
    11401 cagcaacgga tcctgttagt acgtcgacaa aatcaaagcc atttcctttt acttttgcaa
    11461 tgatcagcca agtaatcatc gcaaatgcaa gagcgatgaa tgttgaacca aatgcattga
    11521 ctgcttgttc atctgctcga agtgcaccac cggaattaaa accgaaccag ccaaaccaaa
    11581 gcagtcctgt accgatagca gctgccataa gactgctatg ctgacttctt tctcctaaag
    11641 gaatctttct ttgaccaagt gtgaggatac atgctaaaga gccgaatcca gctgtcgtgt
    11701 ggatgactgc tccaccagcg aaatctctaa atcccatctg atccaaaaat ccgccacccc
    11761 atatccagtg gcaaacaggg aaataaatca gcagattcca aaaaataaca agtataatat
    11821 agccttttaa attcaatctc cctgcaaaag ctcctgtcat caaaggaacg gtaatcacga
    11881 taaacatcag ttggtaaaga aagaacatta aaaatggaat cgttgcacca tgaaacatgt
    11941 tggggaagct tgtaacatgt ctcatcaaaa agaaatctgc cgggtttcca ataacaccgc
    12001 cgatatcccg tccaaaggcc aatccgaatc caccaaatac ccacataatc gttgttatcc
    12061 caacggagag aaaagattga gacatgatcg ttagtatatt attcttccgt gctaatccgc
    12121 cgtaaaaaaa agctagacca ggagtcatca ggcaaaccat tgctgtacat aaaatcatga
    12181 atgctgtatc tcctgtattg ataggtaatg tattcatttt tttcgcctcc tttctacgat
    12241 tacgagtata aaagaaggtg tagttgtttt tttcttctgt ttttttgaat taatacttgc
    12301 atttataaaa gacagacaat taataaaagc ttgatgtgtt gtgctctttt tcgtttttca
    12361 cacaaataaa gacaaaaaat aaacggtctt ctttttatag aaaaccgttt attttcgtct
    12421 ttttttattt ttaaagatat tttttcataa ggaataaaga atacttcata taaaatatat
    12481 aaaaaagaac cgcagcgagt gtcgctacgg ttctcaaatt tgttcttcat ttcaacataa
    12541 gtttttcttc aatttgctta taatttacgt gcaaagaatg aaacgactaa tactaaaatg
    12601 attgcaccga tcaatgatgg aatgatagcc attcctgcaa ttgttggtcc ccaagttcct
    12661 agtaaagctt gtccaattgc tgaacctact aaaccagcaa tgatgtttgc gatgatcccc
    12721 attgatgatc ctttattcgt gattgctcct gcaattgctc caatgatacc gccgacgatt
    12781 aatgcccaaa taaatcccat tttaaattcc tcctaactga cttcttactt cttattgtac
    12841 acgtggttcg ttattttctt tgactttttg tgcacctttg cctacagcag attttgcttt
    12901 atctgtttgg cgtgaagcaa attgtcctgt ggattttgct gcttctgtta ctttgtcttg
    12961 tactgtttct tgatcttttt ggtattcttc ttgtgttttg atgtctgcaa cattcacatt
    13021 cacttcgata acatctaaat gtgtcatatt gtttacttca gtactgatca attctttgat
    13081 ttggttgtaa atatcttcga tgtctttacc gtattctacg atcacatcta gatccacagc
    13141 tacttgtttc ttacctactt ctgtattgat acctgctgtt acgttatctg tgttgactaa
    13201 tttttcagcc atatttgaga aaaatccgcc atctaatgtc aatagaccat ctacatcttt
    13261 caatgcataa ccgatgattt tttggatgac tttgtcttca taagtcaatt caccattcat
    13321 catgcggttt tctccttttt catttgccaa aactgatcca tttgttgtat tcatgttttt
    13381 gttttccatc tttgtttcct cctatgattt aaaaaattga tcaactattc ctgactgttt
    13441 caaataaaaa ccgattcctg cgccaattgc tgtcatgatt atgatcaaca atgtcttgaa
    13501 aaatccaatc gttaaccata aaacagctag cagtagtcca agacccccaa agataatagg
    13561 gagcttgtat gctgaaaata gttctttcat tatttggccc tcctattcca ctcgagagtg
    13621 cttgtagtct cttgtatctt cttgttcttc ataaccgctg tatgtgactg ccactttgac
    13681 cgtttctttt gttccgagta ctttagttac ttcttcttcg atatcttgca tcaatacacc
    13741 tgttttccct actaatgaag aagttcgttt caagtctccg ttgacatgga tatggattcg
    13801 atttttcgtt gaacgtatcc gtactttagg tgaatgaatg aactcttctt cgtgtaaatg
    13861 ggaacgtacc aacccttcga tcgctttttt gtcgattgtt agtttgccat cttcccgttt
    13921 cataacgaaa gttcctcttg actttggata gaaaagaacg actagcaaga tgataatagc
    13981 aagaacggca aatactacag ctgcccagaa aatatattgt tgcatgtagt aattcgttaa
    14041 agtaaagaag cgaaatcgtt ccaattgttc aggcataata tacggcgcat tgatcaacgc
    14101 aatcgctaga aatactgaaa gtagcagtaa actcatgata acaccgatta tttttaaccc
    14161 cttattcatt ttccacctcc tataaactta tttttaccct gtaaataact ctaccgacaa
    14221 tcacagtata gatagcgaac agttagcata gcatcagtaa ataaagcgct accaaacatt
    14281 gctggatcat aacggcgttt tattgccagt ctgatactga aataacaatt gcttacttat
    14341 tctctattta cttttgattg ctggctatac ttaaaggata ttattcaaga ttaaaaattg
    14401 caaagattat gctcagttat tattacaaga ttctatttat
//
LOCUS       DACGLW010000076        14257 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.079, whole genome shotgun sequence.
ACCESSION   DACGLW010000076 DACGLW010000000
VERSION     DACGLW010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14257
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.079"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            complement(161..2158)
                     /gene="tkt"
                     /locus_tag="IXT53_002237"
     CDS             complement(161..2158)
                     /gene="tkt"
                     /locus_tag="IXT53_002237"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HAQ4906034.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2260..2511)
                     /locus_tag="IXT53_002238"
     CDS             complement(2260..2511)
                     /locus_tag="IXT53_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HAQ4906035.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2658..3281
                     /gene="lexA"
                     /locus_tag="IXT53_002239"
     CDS             2658..3281
                     /gene="lexA"
                     /locus_tag="IXT53_002239"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HAQ4906036.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3461..3679
                     /locus_tag="IXT53_002240"
     CDS             3461..3679
                     /locus_tag="IXT53_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906037.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3968..4480)
                     /locus_tag="IXT53_002241"
     CDS             complement(3968..4480)
                     /locus_tag="IXT53_002241"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HAQ4906038.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4494..6791)
                     /gene="recJ"
                     /locus_tag="IXT53_002242"
     CDS             complement(4494..6791)
                     /gene="recJ"
                     /locus_tag="IXT53_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HAQ4906039.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6933..7367)
                     /locus_tag="IXT53_002243"
     CDS             complement(6933..7367)
                     /locus_tag="IXT53_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HAQ4906040.1"
                     /translation="MKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPLILV
                     IIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQREFRN
                     ERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7377..8165)
                     /locus_tag="IXT53_002244"
     CDS             complement(7377..8165)
                     /locus_tag="IXT53_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ4906041.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8162..9103)
                     /locus_tag="IXT53_002245"
     CDS             complement(8162..9103)
                     /locus_tag="IXT53_002245"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HAQ4906042.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9280..9867)
                     /locus_tag="IXT53_002246"
     CDS             complement(9280..9867)
                     /locus_tag="IXT53_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HAQ4906043.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10088..11401)
                     /gene="obgE"
                     /locus_tag="IXT53_002247"
     CDS             complement(10088..11401)
                     /gene="obgE"
                     /locus_tag="IXT53_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003126661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HAQ4906044.1"
                     /translation="MSMFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGG
                     DVILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAET
                     GALLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVL
                     ADVGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLE
                     RPQIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNAT
                     ADLLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTN
                     FDHDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11698..12708
                     /gene="gap"
                     /locus_tag="IXT53_002248"
     CDS             11698..12708
                     /gene="gap"
                     /locus_tag="IXT53_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HAQ4906045.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12793..13728)
                     /locus_tag="IXT53_002249"
     CDS             complement(12793..13728)
                     /locus_tag="IXT53_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ4906046.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
     gene            13914..>14257
                     /locus_tag="IXT53_002250"
     CDS             13914..>14257
                     /locus_tag="IXT53_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ4906047.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERSVS
                     CGMRTLYRLFSKG"
BASE COUNT         3980 a         3165 c         2519 g         4593 t
ORIGIN      
        1 tcaagaaaca caaactaatc cgaagaattc tttgttttgt catttaaatg taacatagcc
       61 gaaaacgaaa agtaatattg atttattctt ctaacacata aagaacagct gtctccaact
      121 tgcagacagc tgttcttatc agtcaaacaa ttgcttggat ctatgatagt tgattgtacg
      181 tattcacgac attttctact gtaaatccaa attcttctaa aattttagtg ccaggggcag
      241 atgcaccgaa atgatcgatt gtaatcgttg cgccctctgt tcctacgtag cgttcccatc
      301 caaatgatgc agcagcttcg atagccacac gtttcttcac tgatttagga agtacacttt
      361 ctttatattc agaagattgt ttttcaaaga ggtcaaagct tggcatagaa acaacagaaa
      421 catctttgcc tttttcagcc aattcttttt gagctttcac agccaaatca acttcagaac
      481 ctgttgcaat caaaatacct tctggtgttt ctccttgtga tggagaaaga acatacgcgc
      541 cttttttcac catttcatct gctacttctt ttgtagaagg caatactgga aggttttgac
      601 gactcaatac aaggatagta ggcgcatctg ttgtctccat cgctactttc caagcagcac
      661 gtgtttcatt tccgtctgcc ggacgaatca cttgaacacc aggcatgcag cgaacagatg
      721 ctagttgttc aatcggttcg tgcgttggtc cgtcttctcc gacagcaacc gagtcatgtg
      781 ttaagacgta aacaacagga gtatgttgga ttgctgctaa acgaactgct ggtcttagat
      841 aatcgacaaa gacaaagaat gttccaccgt agatacgagt tccgccatga agttggatac
      901 cattcatagc cgaagccatt gcaaattcgc ggacaccgaa ccagatgttg cgtccttcat
      961 aatgttcagg agtgaaatct ttatctgcag ccaccattgt attattggaa cctgacaagt
     1021 ccgctgaacc accccagaag cttggtatag ctttcgataa ttcttggatg acttctttac
     1081 ttgaaacacg gcttgcttga ctgcttccaa cttcataagt tggcaattct gcatcccagt
     1141 tttctggtag tttgccatca aaagcatctt caaattgttg tgctaattca ggataagctt
     1201 ttttataatt ggcaaacatt tcattccatg catcttcagc tttttgtcct tcttctatca
     1261 tcgtttgatg gaagcgggca gctacttctt ctggaacagt gaaatctgga tactcccaac
     1321 cataaacttc tttagccatc ttgatgccat cttcaccaag aggagcacca tgaacagcag
     1381 atgttccttc tttaggagca ccatagccga tgactgtttt tacttcgatc aatgttggtt
     1441 tgtctgtttc agcttttgct tcttcaatcg ctttagagat tgcttctagg tcatttccgt
     1501 ctttcactaa gatatgttgc cagccatatg cttcatagcg agcaccaacg ttttctgtga
     1561 aagcttttga agttggaccg tctagtgaaa tatcgtttga atcgtataaa acgatcaatt
     1621 tccctagttt catatgacct gccatcgaac ttgcttcttg agatacccct tccatcaagt
     1681 ctccgtcacc gcatattgca tacgtatagt gatccatcac attaaagttt tctttgttat
     1741 acgttgctgc aaggtgcgct tctgccattg ccataccgac agccattgca atcccttgtc
     1801 ctaatggccc tgtagtggct tctacaccat ctgtatgatg tacttcagga tgtcctggtg
     1861 ttttagaatc ccattgacgg aattgtttca aatcatcgat tgtcacttga tagccagcta
     1921 aatgaagcaa gctatacaac attgctgacc catgtcctgc cgaaagaacg aaacggtcgc
     1981 ggtcagccca gttttttgat gtcgttggat ttactttcag gtgttttgtc cataacgcat
     2041 atgccatagg tgctgcgccc atcggcaatc caggatgtcc tgagtttgct ttttggactg
     2101 cttcaatact taatgtacga attgtgttaa cgccaagttg gtcaattttg tcaaacaaaa
     2161 taacccctcc tattaattta ggtttttttt acctttattt cttacacttt cattctataa
     2221 gatgtcacac gaaaaagcaa tctttttcag tattttttac taaaagttgt catttcggtt
     2281 gtgtaagcct tttttctttt ggatttcttt taatttttcc ggggtcacgt cattgccctc
     2341 aggatcgacg actttcattc cttcgatatg atgacgcata ccggaacgaa aagcttccaa
     2401 atactctttg cgcaagttga tttgttcttg ttcttcggct tttgagagac cattttcctt
     2461 ctttttacgt gcaagttcat tgatccgctc tattttttca ggtgacagca tgctcagccc
     2521 tccttttact ctccttcata ttatataaaa actgaaaaaa aaacaactag ataaactaaa
     2581 taaaatacga acattcgttt gatttagcgc tttttatatg gtactattta tttatcaagt
     2641 gaaagaaggt gtaccttgtg gcccgacaaa ctgaatcaag acaaatcgaa gtcttacgat
     2701 atatccatga acaagtaacc caaaaaggat acccgccgac tgtccgtgaa atcggggaag
     2761 ctgttcagct ttcttctact tctactgttc acggtcatct tgcacggctt gaaaaaaaag
     2821 ggtttatcca aagagatccg acgaaacctc gtgcaatcga gttgacaaaa caaggtttag
     2881 aaaagattgg gattcgtcca acaactattc cagtacttgg cgtcgtaaca gctggagaac
     2941 ccattttagc agtagaggat gcttcagact tctttccaat ccctcctaat ctttcttccg
     3001 aagaaggcag tctctttatg ctgacaattc gtggagatag tatgatcaat gctgggatat
     3061 tggatggtga caacgtgatc gttcggaagc aagaaactgc tcaaaatggc gatattgtca
     3121 ttgcaatgac tgccgaggac gaagcgactt gcaaacgatt ctataaagaa aaaaactttt
     3181 tccgtctgca gcctgaaaac gacttgctcg aaccaatcat cttagatcaa gtatctatat
     3241 taggacgagt agtaggactt tatcgtagtc atatctatta attgctacta attaaaagaa
     3301 aactttttaa aaagagtttg agacgttggt ctcaaactct tttttcgccc gggttcttct
     3361 tctttcaaat ttttatcact tccctcttta caaaccgaac aaatattcgt atactaaata
     3421 tacgaacaat tgttcgcata aattaaagag gtgagaattc atgaaatcaa tgagacgtct
     3481 tggcttagta ttaactgttt tgtttttagg tattattatt ggtaatttcg gcatacgtcc
     3541 agctttactt gtgtttgttc cgttatttac tttgtggttc atgttgtggg atgaaaagaa
     3601 gtaccgtcaa tctgagcggc gcagaattca tgaagatcat ttctatcatg agccctacta
     3661 cagatccagg caatcataat aaggattgac tgctaattat tcaaaagaca agaagttaaa
     3721 cttttacggg ggtgtgacaa aaatcggtca catccttttt ttcgaataaa cggtgttcta
     3781 aagccagttt ttcggtatta agtcaaattg cggaaaaagt gattttttaa acttctactg
     3841 gtctttgctt gtttccattt ataaaaaaat agcgaccctc aaaagtcaga tagagaatct
     3901 gacttttgag ggtcgcttta aaatcgcttc tttaagaata acatcaagaa tagcttcttt
     3961 tttctgctta atattccatt aatgtaatga tgtcatatcc tttgattttg tcgcgtccat
     4021 ggagatccat caattcgatc aaaaatgcgc agcctacgac tacaccgcct aattgttcga
     4081 tcagttcgat cgtcgctttg attgttcctc cagtagctag caaatcgtca caaatcagca
     4141 cacgttgacc tggtttaatg gcatccttat gcaatgtcaa tgtatctgtt ccatattcta
     4201 atccataagt cacttcgatg gtttctcgag gtaacttccc ttttttgcgg actggggcaa
     4261 aaccgactcc taactcataa gcaacaggac atcctacgat gaatccacgg gcttctggcc
     4321 caactaccat atcaattcgt ttttcttttg cataatttac gatttgttta gtagcttccc
     4381 gataagcgtc accatctgcc atcagtgggg aaatatctcg aaacacaatt ccttttgacg
     4441 gatagtcggg aatactggca atataatctt ttaaatccac tctttgtttc ctcctaggtt
     4501 gttaaccatt tctttatggt aggaatatcg ctcaaaagca aaaattcctc aactttgatt
     4561 ttattgagcc gtcgctggta taatctgctc tcagtcaaag gatgactttc tgggctagcg
     4621 actttttgta aaacaccgtt ctctattgta acaaatttca attcaaaaaa cacctgtatc
     4681 ataaaaatta ataatttttc agggatacgc aaatattgag ctattgcttt tattttatga
     4741 cgtatatcaa ttcgttcttg agaatggatc aatttaaaca attttgcgta ctgttctctt
     4801 gtgcctaccc cattcaaata ggcctcatca ggtgaaatcc ccatcagata aacacgattg
     4861 aatgaataag accgaaaaac ttctttcaga tccgctaaat catctggaca atccataaca
     4921 actagagagt ttggttttct tttttcaatt attgccttta cagaatctag tccttcgaaa
     4981 acaatcgcag cttcctggat aatctcaggc acgaatttca acgtcgaaga atcaaatgct
     5041 aaataaaggc taccttccga cgttcggctc tgttcacgga aacgttttgc ccgccaatca
     5101 aatacttgca gaccacccac agaaaaatca gataccatca gctgcggttt cttacgcccg
     5161 ttccattcat tgatcgaaag ctttcctacc acatctacct gattattcag cagttcttct
     5221 tcttgagtac caaaaccaaa agcgaccgca tcaagctgac tcgatgcatc ggaaaggaca
     5281 aatttcagat gctgttggtt tgctccgatt cttttgatat tttctgcttg gacttgtcga
     5341 aataaaaact gaggcaaagg attatccgta ccaaatggcg ccaatagttt cagctcatca
     5401 attcgctcta ctgttacttc atttggcagc aatacttcat caattctcaa ctcaggccct
     5461 ctcatcaagt caatttgatg atcgactata tattggttca tcttctcctg tagtatagtg
     5521 acattttcac ttggcatcgt taaaccgact gctgcatgat gtcctccgaa gtaagtaaat
     5581 aaatctcgca tctggtcaag catttcaaaa agattcaagg cttcaatact acgtccagat
     5641 cctttagctg atccatcttc ttttaaagta aggacaagag ttggtttacc cgtttcattc
     5701 attattttcc cagcaacaat gcccaaaacg ccttcatgcc atcctcggtt taccaataga
     5761 tggatatgat tctccttatt gaccatcgct aatgcttctt gtgttatctg ctctacgatt
     5821 gcttttcttt cttcattgat ctcattcaat ttttttgctt gaacggatgc ttcctcttca
     5881 tcaaacgtcg caagtaacga gacggcagga cgtggatcgc ccatccgacc gattgcattt
     5941 agtctgggag caatcgaaaa accgatcgtt gtttcgtctg cgtccctcat ttttacgccg
     6001 ctttcttcaa atagcgcctg aagtcctatc cgttctgaat gatgaatggc atcgattcct
     6061 aatgaaacaa ggattcgatt ctcatcagtc aatgaaacta aatcggcgat cgttcctatc
     6121 gctactaaat ccaaaaattc tgcaggcggt tcttccaata atgcagaagc tactttgaat
     6181 gctactccga ctcctgccaa atctggaaat ggatagtgcc ccgctggatg acgagggtga
     6241 acaatcgcat aagcgtctgg taaattctca ggtagttcgt gatgatctgt aatgatcacg
     6301 tctacaccag cttgctgagc gtatgctacc gcatcatttc ctgctacccc gttatcaaca
     6361 gtgatgatca actggattcc ttcttcgatc ttttcttggt ataccgattg gtttggtcca
     6421 tacccatgct caaatcgatt tggcaggaat acttctactt ctgcaccaag taattccagt
     6481 gtttctttca ttaccgttgc actggtgatc ccatctgcgt cataatcacc gtaaacgagg
     6541 attttttctc cactgattac agcttcttgg attcggctga ttgctttatc catatcgtgt
     6601 atcagatacg gatcatgtaa ttcttgttcc tttggatgta aaaatcggtt gatatccttt
     6661 tcctcatggt aacctctttg ccacagtaac ttaccgacaa atggagataa tttcagttca
     6721 cggatcattt ggagaaagga ttcagaaggc tctgtctcgc ttaacttcca atcaaaattt
     6781 gcttgtctca ctttctcact cctaacttag ttattatagc aaaggaaatc agcaaaaaaa
     6841 agcagaaaga aagaataaaa acagatcaga tccgctagac agatgttcag caggacctga
     6901 tcttttgtac gaccatttat gaaatatccc tattagtcaa aataattgaa gctttgtttg
     6961 ggcgattgag ttttttgttc ttccggcgct ttcaattgac tgatttgtgc ggttttcgct
     7021 tgtaactccg cctgataagc agcttccaac tccgcacgtt cgtttctaaa ctcgcgttgc
     7081 tgttcttctt ttgcttcttc aattttcttt tgaagttctg tttgatattc tgacatttcc
     7141 tgagtcagct gtttgatttg ctttttttgc tgccatagtg ttgccgaagc agtcaaaaat
     7201 acaatcagtg caccaatcag cgcagaaccg atgatcacta agatcaatgg cgcagaaaag
     7261 gaagtgaagc caaaacttac aggaacattt ttattgttca atacagcaaa aagaacaata
     7321 atcaaaacta aaacaaatcc aagaatcaca ttgctttgct ttttcaattc cttcacctac
     7381 tttttgttaa aaattccgct ggctaaataa tcaccaatcg ttggaaacag cgtataaaat
     7441 tttgatgcaa cttctaaaat aaacggttga ttgatttccc gttttggatg tctcatattg
     7501 cggacgatca tttttgccaa cttggcaggt tctaaaacga tttgtccgac tttttccaaa
     7561 tagcttccgc ttggatcagc cttatcgaag aaatttgtct cgattggacc tggattcact
     7621 gtcgtaacag ctacgccaag tggtttcaat tctaatcgca aagcgttgga gaagccaagg
     7681 actgcaaatt tcgtggcaga ataaaccgtt gattttgcag ttgccatttt tccagccata
     7741 gaggcgatat taatgatatg accttgtctt ctttctgcca tatcgatcgc aaatttttgt
     7801 gtcaaaacca tcattcctaa gatgttgact tcaaacatat cgtatgcttt tccaaggtcg
     7861 aatgtaagga agtcttcgaa aatcccaaaa cccgcattgt ttaccagaat atcgacgcgc
     7921 ccgacttctt ccatcacctt ttcatataat tcgtccacgc tttcaggatt gctgacgtct
     7981 accttgaaag cataagcctc tttgccgctc aagcgtctgc actcgtctct gacaccttct
     8041 acaaatgcca ttcttcgtgc gcaacttata acgattgctc cttgctttgc tgcttcataa
     8101 cagatttgtg cgcccagacc gcctgaacta cctgttacta aaacaacttt atcttctaaa
     8161 ttcatgcctc ttcctcctcg cgaaatggaa tctcaatgct atcaaaatct ttcataatcg
     8221 tcgtgttttc aaacacttct tgtgcttctt tttcaagctc taaagctgct tttcctaaat
     8281 atctcgcact gatatgagta agtagcagcc gttttgcctg cgcttctttt gcaacttcag
     8341 cagcttgatg agtcgtggaa tggaaatagt tgtgcgccat tcttgcttca tctttattaa
     8401 atgtgctttc atgtaccaga atatcgctat tttccgcaag cacaacggaa ttatgtgttt
     8461 ttctcgtatc gcctaaaata gtcacgattc gtcctttttt atctggacct acgaaatctt
     8521 ttccattgat cgtccgccca tcttctagtt ggatcgtttc accttgcttc agtttgccgt
     8581 acagtggtcc agctggaata cctaattctt ttagacgatc cacctgcaat tctcctttat
     8641 gatcatgttc aaccacacga taaccaaaac tgtcgatccc atgatttagt ttttttgtat
     8701 aaacagaaaa ttgctgatca gtaaaaatcg gggctgtttc atttagctca atgaatttca
     8761 gcggataact taaacgcgtt tgcgaaatac ctaaagagac ttttatgtat tcctctatcc
     8821 cttttggccc gtaaatctct aatggcgtat ccccaccttg aaatgaccgg ctgctgatga
     8881 gcccgggaag tccaaaaata tgatcgccgt gtaaatgtgt aatgaaaatt ttaccgattt
     8941 ttctcggacg aatagtcgtt cgcaaaatct gcatttgcgt gccttcgcca cagtcgaaca
     9001 accaaacttc attgcgctcg tctaaaagtt tcaatgctat acttgtcaca tttctatgtt
     9061 ttgcaggaac acctgcacct gttcctaaaa attgtaattc cataatctac ctgactttct
     9121 ttaagttcct ctttaatttt agggtaaaaa gaagggataa tcaaccgtaa gtcgagaatg
     9181 ggttcaaatt aaaaataacg ttctaaagcg atagcgtttt ctttgaaaat tactataaaa
     9241 acagcagcga acccgtttga gatcctctgc tgttttttat taatttgaag atccagaact
     9301 ttcattgtcg ttggaagttt gcggatgttt gtccttccaa gtcttttgtg cagcttcttt
     9361 cgcatgattt tcagcttctt tcgcatcatg cacatctttt tcgacatctt cataatccaa
     9421 gcgagtaatt tctccgccta attccatcat taccaattga ttcagtgggc gattgtcctc
     9481 ttcatcagca atcaagatca gtcctgtagc tccttcaccg attttatttc ctacaaagtc
     9541 gaacacggtc tgggcatctt ttatttcttt ggcatcttgt gtcgcaccga tcattcctcc
     9601 ggcaaaccat cctaataaaa tccctagcgg accacctaat acaccaacca acattccgat
     9661 taatccaccc gtagaagatt tgttattacc ggtaaagtcg ataaaatctt ctattttgaa
     9721 ttgatgtgtc ccattaggat catggctgac aactgccatc tgttctcctt taatcgaacg
     9781 aacagcatgt aactttttga tttctgaaaa tgcctgatat gcctggcttt ctacttgaaa
     9841 atttaaaatg attactcgtt tgctcattaa aaacgcccct ttcatacctt cttgttattt
     9901 attttacctt taattgtaaa ataagaaaag aaaagcactc tttttttcca ataaaatagg
     9961 gctgtgacaa aagtcgtatc acagctcctt tttccgaata aacggcgttc ttgaagctga
    10021 tcacttctgt cacaacctcc cttaaatctg aatctatatt ttttggtatt tgtttttatt
    10081 tgcttgccta ttcgacaaat tcaaattcaa actcaccgat acgtacaagg tcgccatctt
    10141 tcgcaccgcg tgcccgaaga gcttcatcca cacccatacc acgtaattga cgagcaaaac
    10201 gcatcactgt ttcatcatga tcgaaattcg tcatttggaa taatttttcg atcttctcac
    10261 cagataagat ccacgttgca tctgaatcac gatcgatttg gaactcagga ccttctggat
    10321 tgaatccata gtgtacagtt tcttcttcca gttcttcttc gtacaatggg aattctggcg
    10381 ttacttctaa caaatctgct gtagcattca ataacgcgtc caatccttgt cgagtcactc
    10441 cagaaattgg aaatactggg atgtcatcag caaattcatc ttctttttcc ttgttcagct
    10501 gttctttgaa tttcactaga ttttccggtg catccggcat atccattttg tttgccacga
    10561 tgatttgcgg acgctctagc aagcgtaagt tataagtaga taattctttg ttgatggcaa
    10621 gatagtcttc ataaggatct cgtccttcca tcccactcat atcgatcaca tgtaaaatta
    10681 ctcgtgttct ttcgatatga cgaaggaatt gtgtacccag cccaacacct tgcgaagctc
    10741 cttcaatcaa gcctggcaag tctgctgcgg caaaacttct gccatcactt gtagtaacca
    10801 ttcctagatt aggtacgagc gtcgtgaaat gataagcgcc gatttttgga cgagcagatg
    10861 aaataacgga tagtaaagtc gatttcccga cagatggaaa acctacaagt ccaacgtctg
    10921 ccaatacttt caattctagt tcgatttttc gctcttgtcc tggttcaccg ttttcagcaa
    10981 tttccggtgc aggattccga ggagaggcaa aacggatatt ccctcgtcca ccgcgtccac
    11041 cttttgccac aactagtgtt tgaccatttt caattaaatc tcctaaaaga gcaccagtct
    11101 ccgcatctcg gacagtggtt ccttgtggta ctttaacata ggtatcttct gaaccacgac
    11161 cgtgcatccc tttactcatc ccattttcac ctggctgcgc tttgaaatgg cgattgaacc
    11221 gaaaatccat caatgtacgt aacccttcat cgacgatcaa gatgacatcg ccgccacgac
    11281 caccgtctcc gccagccggt ccgccgtcag gaacgtattt ttcgcgacga aatgcaacca
    11341 ttccgtctcc gcccttgcca gctttcacat cgatcgttac ttgatctaaa aacatggaca
    11401 taattaagtc ctcctattat tcatttattt atttgactgc ctgaatttcc acaataaaca
    11461 aaagtcttac ttattttatc ggtgaaatct gattttgtct atcatttcag caaaaagatt
    11521 gtttaccttc ttcttttttt atcatttggt aaggtttcca accttttttc tgctaccaag
    11581 tataattttt tgacaaaata ttctttgcgt agcagaataa cagtgtatta attgtattaa
    11641 ttgtattaat tgaggatgcc gtttcacagc acccctaata aaggaggaac ttttgatatg
    11701 acagtaaaag taggtattaa cggatttggg cgtattggcc gtctggcttt ccgccgcatc
    11761 aaagaagtct cagatgatat tgaagtagta gcaatcaacg acttaacaag tccaactatg
    11821 ttagcacaat tattgcaatt tgactctact catggtactt atcctggcac agttactgca
    11881 acagaagact caatcgtagt ggatggcgaa gcaactcgtg tttatgcaga acccgacgca
    11941 agcaaaatcc cttgggcaaa agaaaacggt gtagatatcg tactagaatg tactggtttt
    12001 tatacatctg aagaaaaagc aaaagcgcat ttagatgctg gtgtcaaacg agtagttatt
    12061 tctgccccag ctggtgctat gaaaaccatc gtctataacg tcaatgatga tacattagat
    12121 gaaaacgaca aaatcatctc agcaggttca tgtacaacaa actgtttagc acctatggct
    12181 tatttcttaa ataatgaatt tgggatcgaa gttggtacaa tgacaacagt tcatgcctac
    12241 acttccacac aaatgctctt agatggacct gtaaaaggcg gtaatttgcg tgctgctcgt
    12301 tcagctgctg acaatactat cccacattct actggtgctg cgaaagcaat cggcttagtt
    12361 atccctgagc tacaaggaaa attacaagga cacgcacaac gtgttccagt cgttgatggt
    12421 tcattaactg aattggtttc agtattgaaa acaaaagtaa ctgctgatca agtaaacgaa
    12481 gcaatcaaaa aacacacagt tgacaatcct tcattcgggt atgatgaccg tcaaatcgtt
    12541 tctagcgatg tcattggaac aactcaaggt tcgatctttg acccaacgca aacagaagta
    12601 acaactgctg gtgatttcca acttgttaaa actgttgcat ggtatgacaa tgagtatgga
    12661 tttacttgcc aaatgattcg cttactagaa aaattcgcta acttgtaatt tgatagatca
    12721 atcgataaaa gaagcctttg actggatcca gtcaaaggct tctttctttt catcccctac
    12781 ttttaaactc gttcaatgaa catattggct taaaaattgt tggactactt gctgatattt
    12841 ttcaggatct gtcttgtatg cttccgcatg ctcggctcct tttacaacat acttttcttt
    12901 tggtcccttc gttgcacgat agaccttatc taacatttca aatggtacaa atgtatcttc
    12961 gtctccatgt ataaagagca ttgggcgctg gtttttcttc aattgttcca ctgcatttgc
    13021 ttcaccgaaa aagtagccag cacggacttt tgtcactaga ctcgttactt gcataagcgg
    13081 aaatgctgga agactaaaca tatcgtctag ctgataagca agctcatcat gaacggaaga
    13141 ataaccgcaa tcttcaatga ttgctttcac attatctggt agtttttctc cgctagtcat
    13201 cataactgtt gctgctccca tgctgacacc gtatagaacg atttcttgcg attttccatt
    13261 gttttctagc actttgttta tccactgtac atagtcttta cgctctggcc atccaaatcc
    13321 aatgtaatcc ccttcgcttt ttccatgtcc tcttgcatca ggaacaagga cattgtaacc
    13381 catatcatga aacatctttg cgtaattggt catcgtttca gcgtttccca tatagccatg
    13441 agccacaaga gctgtttttt cacttttcgt ttcagcagga agataaatcg ctgataattt
    13501 cagaccgtct tttgaagtga tggtccaatc gcttcggttt tcttttgaac taaaccattt
    13561 atgtgcatcc gtttctttgg atgtctgtgt tgtaccaggt gtatcttcgt ctaaaaagtc
    13621 tttctcagaa ggcacaacag cataattata aaaataattc cctgcaaaca ctaacgcaac
    13681 aatcagagct aaagcgacgc ctaaaaggat tttcatccaa agtttcataa atcatccatc
    13741 tcatttctct tttttcatat aaccttgaat tagtataatc aaagaatctc tactatgcaa
    13801 tgttcatttt tataacccgc gaattgtaaa attaagttag aaaaataaaa aggcatttat
    13861 ggtacactca aattgtattt ccgacgaaag aaaacaaagg agtgtaacca taaatgacct
    13921 acaaacatct taccatagac gaactgacaa tgatagaatc atattatctt caacataata
    13981 agccggttga aatcgctaac cgaatgggac gtgctataca aactatttat aatgtagtca
    14041 ataagttcaa gcaaggcaag actgctcttg attattggca ccagtataaa gaaaataaga
    14101 aaaaatgtgg tagaaaagtc attcaattac ctgctcatga agtagattac attaaagaga
    14161 aagtcactct tggttggacg cctgacgtca ttatcgggcg aaaagaaagg tctgtttcat
    14221 gcggtatgag aacactttat cgtttatttt ctaaagg
//
LOCUS       DACGLW010000077        14118 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.080, whole genome shotgun sequence.
ACCESSION   DACGLW010000077 DACGLW010000000
VERSION     DACGLW010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14118
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.080"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            complement(<1..556)
                     /locus_tag="IXT53_002251"
     CDS             complement(<1..556)
                     /locus_tag="IXT53_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016182552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HAQ4906048.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGA"
     gene            complement(572..1051)
                     /locus_tag="IXT53_002252"
     CDS             complement(572..1051)
                     /locus_tag="IXT53_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HAQ4906049.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKARLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(1057..2081)
                     /locus_tag="IXT53_002253"
                     /pseudo
     CDS             complement(1057..2081)
                     /locus_tag="IXT53_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            complement(2098..3204)
                     /locus_tag="IXT53_002254"
     CDS             complement(2098..3204)
                     /locus_tag="IXT53_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HAQ4906050.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(3433..6030)
                     /gene="adhE"
                     /locus_tag="IXT53_002255"
                     /gene_synonym="adhC"
     CDS             complement(3433..6030)
                     /gene="adhE"
                     /locus_tag="IXT53_002255"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HAQ4906051.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(6335..6715)
                     /gene="yajC"
                     /locus_tag="IXT53_002256"
     CDS             complement(6335..6715)
                     /gene="yajC"
                     /locus_tag="IXT53_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HAQ4906052.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(6812..7957)
                     /gene="tgt"
                     /locus_tag="IXT53_002257"
     CDS             complement(6812..7957)
                     /gene="tgt"
                     /locus_tag="IXT53_002257"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010769586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HAQ4906053.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            8175..8873
                     /locus_tag="IXT53_002258"
     CDS             8175..8873
                     /locus_tag="IXT53_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HAQ4906054.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(9041..11680)
                     /locus_tag="IXT53_002259"
     CDS             complement(9041..11680)
                     /locus_tag="IXT53_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ4906055.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(12299..13330)
                     /gene="queA"
                     /locus_tag="IXT53_002260"
     CDS             complement(12299..13330)
                     /gene="queA"
                     /locus_tag="IXT53_002260"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HAQ4906056.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            13523..>14118
                     /locus_tag="IXT53_002261"
     CDS             13523..>14118
                     /locus_tag="IXT53_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005232177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ4906057.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEA"
BASE COUNT         3959 a         3096 c         2543 g         4520 t
ORIGIN      
        1 aagcgcctac gagataacct cccataaaca aagcagagat cgaagcacca ccagcaacaa
       61 gtgaatacat gataaaagca gtacttggag gaatgaccat acctgtcgga gctgacgcga
      121 tattgaccgc tgtcgcaaac ttccggtcat atccttcttc cacttgctga gggatcaaca
      181 ctccaccaat cgcggtagaa gcggcaatgg ccgagctaga gatggaacca aataaagcat
      241 tccctaacac attcgtatga gccaaagccc ccggcacgcg cccgacgaac aacatcgcaa
      301 aatccactag tttcttagct atccctccat tattcatgat aatgcccgag agaacgaaaa
      361 acggaatagc gatcagcgaa aaactattga tacttcccac cattttttga gctgaactaa
      421 aagcagaaac atcaaacggt ataactaaca ataaagtcat catcgatgag aatgcaatac
      481 ttatagcaat cggcatccct aatacaagca acacaacaaa tacaaaaaat aacactaatc
      541 cggcttctat tgccatttta ttcctccatt tttatatttt ttcttgaatt gctggcttgt
      601 tgcttaatgt atcgattaga ttgatcaggc tatagataat gatgagtacc cccgatacag
      661 gtactgacag ataaacatat cccataggaa tacttaaaga aggcgagatc tgccccattg
      721 tcaatgtgac agcattccat ccgccataca ccatgatcat cgcagcaaat accagaaata
      781 aactttgaac aacaacttct aaaattttac tttttttgcc agttaatttt tcacttaaaa
      841 ccgttacggc taaacgtgct ttttgtccca caacatacgc ggaagacaac atggcaaacc
      901 agacaagacc gaatcgaaca agctcttccg tgaccgtatt tggatttcct aaaaccgttc
      961 tggaaaatac ttgccataaa acaacagcga ccatcccaac aagcaaaaca gctcccagga
     1021 tctctaatac cttgtttaaa atcattttca ttacagctac gctccttgct cttggatctt
     1081 ttgataaagg tctttgtagg taggatcgtt tttgaatata tcatgaagtg gggcaactgc
     1141 ctctcgaaac ggtgtattat ctacatcatt gaattggact ccatgttctt cttgtgctaa
     1201 agctttttct tcttcaattg cttttgcaaa agcttcaatt tcaaaaaccg ttgattcttt
     1261 cgctgcttct tcaatggctt tttcctgttc atcagttagc tgttctttga tggcatcatt
     1321 catgatgatg atatctggca cacgtgtatg ctcatcatag gaatagtatt tagctacccc
     1381 gccatgtcca gctgtataca agacaaattc attgttttct gatccgttga tcaaattgga
     1441 ttgaagagac gtgtacactt catcacttcc cataggtacg ggcgaaccgc ctaaaagctc
     1501 taccattttg atagcggttt cgctttgcat cacacggatc ttctttccct tcaaatcttc
     1561 cggcgtatgg atcggaccgt ctaccatata aaagctgcgc tgaccagaat cataataggt
     1621 caatccggta aatcctaaat ttttggtcga ttggtagatt ggctccatta tttcttcatt
     1681 atccatcacc ttaaagaaat gttcttcatc attgaataga taaggaacgg aaaaaatcga
     1741 ataatcttta gagaaacttt ccaacgctga ggcacttact tttgtgaaat ctactgctcc
     1801 tgactgtgtc aattcgatca gttcacgttc tccacccagt tgtccatctg ggaaatactg
     1861 gatctgtacg gaaccatctg ttttttcttt tactaaacgc ccaaattcat tcattgcatc
     1921 aattactggc tgttgcttgc ataagcaaag cgcaaggtaa ctatctcatt tacgtctgca
     1981 tctgcgacag ttgccacact agaattattt ccgcagccgc ttagacctag cagacctagg
     2041 ctaagcgaac ttgctgcaaa cagcagtgat gtttttttca tatgggtttc ctttctttta
     2101 acaataagag acgagcaatt ctgtcaaaca aagcaccgtt aacgactgtc cataaggcat
     2161 tgctgtgatt ccgatattcc gataaaaatc taaactgtcc cccattcctg tcccgattga
     2221 aacgttttga acttctcctt tttcatcgat ttgttcaagc aatccttgga tagcacgata
     2281 agcgacttct ttatattctt gtggtaaata acgtttatgg accgctttca aaataccgta
     2341 agcaaatcct gcagttgcag aagattctac gtaagaagaa ggatcatcca acaacgtatg
     2401 ccataaaccg ctttcagact gataagtttt taatgcacgc acttgtgctt caagtgtttc
     2461 gatcaataat ttgcgcaatg catcttcttt cgataattct aagatttcaa tgatctctgg
     2521 aatagcaatc gtcaaccagc agtttccacg agcccaaaat gcttcggcat agttatgatt
     2581 tccttcaaac gtccagccgt gataccacaa tcctgttcgt ttatccatca agtatttgat
     2641 atggatcata aactggtaac gagcttcttc taaatattct gggcgattca acagtttccc
     2701 gatttttgcc aaaggaagca ctgtcatcat caatgtatcg tcccacaact gattcttatt
     2761 ttccggtcca tatgtggcat gctgtaaccc gtcttcattt gtccgaggca tgtcattcat
     2821 gacccattct gcccattgtt ctaaataagg caaatagcgt gaatctttcg tgtcttcata
     2881 caagtaagcc atagtcaata gcggtgccat tgtattgacg tttttgggcg gagcgccttc
     2941 ttccatacga tcctcaaacc attcattgac cacttgataa gcgcgaggat tttttgtcat
     3001 ctggtaattt ttataaatac catatagacc gacaccttgt ggccaattcc acactgtcca
     3061 gcttttgtca tctactttca gtccatcaaa gtccaacaaa aattcaccat tatcgtcttt
     3121 gatcgttgtc aggttttcaa cgagcaggtc aattttttct ttcactaatt ctttaggtac
     3181 aaaaatctct tctttaataa tcatctttac gccacctttt taaacgcttt cattttatat
     3241 ccttaatata gcagtaatac cgtccactat aaatatcgct ttttgaacaa aaactgtcta
     3301 aaattgacct aaatgttaaa taacaaaagt aactttcagt tacttggaaa ttaaaaaaag
     3361 aacttggaga aacaacgttt tccaagttct ttttcatcta ttttcttatc ttttttctac
     3421 gctttctttt gcttatccgt tgtaagcatc aatgatgatc tgtttcaatt cagagatcaa
     3481 tggttctttc ggattagctg tcgtacattg gtcttcgtaa gctaattcag ccatacggtc
     3541 tacggttgta tctagtgttt cttgtgtgac tccttgagct tttagactca tgttgatgcc
     3601 gacagatttt cctaattcat aaacagccgt tgctaaagct tcgactaatt cctctgttgt
     3661 ttcgcctttc agacctaaga attttgcgat atctgcgtaa tctgtatctg cacggaaata
     3721 gtcatattta gggaacatcg catgtttttg tggatctttt gcattatagc ggataatatg
     3781 cggtaaaaga atcgcatttg tccgtccatg agggatcccg tattccccgc cgattttatg
     3841 tgcgactgag tgacaaatac ctaggaatgc gttagcaaac gccataccag ccattgtaga
     3901 agcgttgtgc attttttcac gtgattccat atcaggtgtt tttactgatt tttctaaata
     3961 gtcaaagact aacttgattg cttgtaggct caatccacgt gtataatctg aagccatgac
     4021 tgaaacatag gattcgattg catgtgttaa cacatccatc ccggtatctg ctgtgacaga
     4081 tgctggaact gacatgacga attgaggatc gacgattgcg acatctggcg tcaatgcata
     4141 atctgctaac ggatatttca catgtgtttc actgtcagtg atgaccgcaa atggcgttac
     4201 ttcagaacca gtccctgatg ttgtcgggat acatacgaat tgtgtttttt ctggtttgtc
     4261 gatcttatat gtccgtttac ggatatccaa gaatttttgt tttgcaccaa agaaagatgt
     4321 gtctgggtgt tcatagaaca tccacattcc cttagctgcg tccattgcag aaccgcctcc
     4381 aagagcgatt actgtatctg gcttgaagtc tgccatcagt ttcgttcctg cgtatacggt
     4441 atttgtcgat gggttcggtt caacatctga gaagacttcg atcttcacat catttttacg
     4501 tcgtgccaat acttcacgaa ctgtatcgca gtaaccgaat tggaccatac cagggtcaca
     4561 aacgatcatg acacgttcaa cgttttccat tttctctaag tacaatagag agttcttttc
     4621 aaagaagatt tttggcggta atttgaacca ttgcatgtta tttctccgtt tcgctacagt
     4681 tttgacattg attaagttta cagcagagac gttacgagaa acagagttct taccataaga
     4741 accgcatccc aaagtcaatg acggaatcat ttcgttatag atatcaccga tgccgccttc
     4801 tgcagatggt gtattcacta aaatacgaca agctttcatt cgaagaccga atttgacatg
     4861 caactcttcg ttttctgaat ggataactgc tgtatgtcct aatcctccaa gattcaacat
     4921 accttcacac aaatcaaacg catggtccgt gttgttagct ttgatcatgg caagaactgg
     4981 agaaagtttt tctcttgata gcggataatc tgggcctacg ccttccaatt cagcaatcag
     5041 cattttcgtt ccttttggta cactgattcc tgccaatttt gcgatttcca tcgcagaatg
     5101 tccaacgatt gatggattga ccgcatattt tccttcgttc atcactgcat cttctaattt
     5161 ttgcaattca tctggtttta cgatataaac ttggtgtgct tggaattctg cttttactgc
     5221 tgcatagatt tctttatcca cgatcacagc ttgttcagaa gcacaaatca ttccattatc
     5281 aaatgttttc gaaacgatca aatcgttcac tgcacgttta atttttgctg ttttttcaat
     5341 ataagctggt acgttacctg gacctacacc taaagctggt tttcctgtag agtaagcaga
     5401 cttgaccatt ccagctccac ctgtcgctaa gacgatcgcg atacctggat ggttcattag
     5461 catagaagtc gcttcgatcg aaggatgttc gatccattgg atacaatttt caggagctcc
     5521 ggcagcgata gcagcatcac gtaccactcg tgctgcttct gcagaagatt tttgggcact
     5581 tggatggaaa gcaaaaacga ttggatttct tgttttcaac gcgatcattg atttgaaaat
     5641 cgttgtggaa gtcggattcg ttgttggagt tactccgcaa accacaccga ctggttcagc
     5701 aatttcaatc aacccttttt ggacatcttc attgatcaca ccaactgttt tatcatgttt
     5761 gatattatgc caaatatatt cggacgcata catatttttg attgctttat cttcgtaaac
     5821 accgcggcct gtttcctcta cagccatctt cgctaatggc atatgttgat ccaaagctgc
     5881 cattgccatt tcatgcacga tatggtctac tttttcttga tcaaagcttt ccatttcttt
     5941 caatgctgca tttgcttttt ctgccaaatc atcaatcatt gcttgtacgt caattgtctt
     6001 cgttttttct ttttctatta ctttagccat cattatcctc ctaatgggtc atggttattt
     6061 gttaaataat tcacatgtat atcatacaat atttattttt ctatgtaaac acttttttgt
     6121 gatttttttc acagttattt ttaaatataa aattgcttta tttacaggtg tttgtataga
     6181 aaaatatata aagttattta ttattattta tatgacacac ccgattattc aatattgagc
     6241 aatatatccc atgctcttat ctctttcttt tttgaataaa aaatgaggta ggacatcttt
     6301 ctgccctacc tcgtctttaa ataagctttg atacttattt ttcttccttc tcttctgtct
     6361 cttccagcgt ttctgttgtt tcagttttta cttcaactgt ctcagctggt tcttctgttg
     6421 tttcaacagt agtcgttgca tcatttgtta cgacagtccc tggtttgacc gttttgattg
     6481 ctgcacgatc gtactcaagg aagatccctt cgcagtctaa aacaactgtc cgtttgtcat
     6541 tgtcgatttc tgaaacaact ccatgtaatc cgccgatcgt tacgactgaa tcacctggtt
     6601 tcatgctatc taacaagttt tgacgttctt gctgttgttt cttttgcgaa cgtgacataa
     6661 aaaaccacat tgctaatagc gcgataaaca tcacaagcat tggtaatgtt cccatttttt
     6721 tcacctcact aattctgtat ctacaaaaaa cactttagca gaaaaatgct gataacacta
     6781 gttttttact taatttttaa aagattttcc tttagaagct ttttgcgttt tctttattga
     6841 acccgtattc ttcaaaaaag gcttctctaa attcaagcaa attatcatcc atgattgctt
     6901 gacgtacttg tttcattaga ttcaacaaga agtataaatt gtggtaagaa gtcaaacgaa
     6961 tcccaaatgt ttcatcacat ttgatcaaat ggcggatata ggcacgtgta tagtttttac
     7021 atgtataaca atcacatttc tcatcaattg gacggaaatc atgggcatat ttcgcatttt
     7081 tcactactag acggccttga gaagtcatac atgtgccatt tcgagcgatt cgagtaggca
     7141 agacacagtc aaacatgtct actccacgga taacaccatc gatcaatgaa tcagcagcac
     7201 cgacacccat caagtaacgt ggtttgtttt cggggatcaa cggtgtagtg aattctaata
     7261 cacgattcat ttctgattta ggttccccga cagataaccc gccgattgag taaccaggga
     7321 aatccatccc gactagatct ttcgcgcttt gacgacgaag atcttcaaac cctgctcctt
     7381 ggatgattcc aaataatcct tgacggtctg gattagcatg agctttcaat cctcgttctg
     7441 cccatcgaga cgtacgttct actgattttt tcacgtagtc gtaactttca tcgaatggtg
     7501 gacattcgtc aaaactcatc atgatatcag agcccaattt attttggatg ttgattgctt
     7561 tttctggtga taaaaacata cgcgaaccgt tcaagtgatt tttgaaatgc acgccttctt
     7621 cttcgatatt ccgcatatca cttaatgaaa aaacttgaaa gccgcctgaa tctgttaaaa
     7681 tcggttgatc ccagttcata aatttatgca gtccgccagc ttcttctact aagtcttcac
     7741 cagggcgtag ccataaatga tatgtattac ttaaaataac gccagcaccc atttctttca
     7801 actcttctgg ggacattgtt tttactgtag ctaatgttcc tactggcatg aacattggtg
     7861 tggggaaagt tccgtgtggc gtgatcaatt cccctaaacg cgcgccagta tgtttttctt
     7921 tcttgattaa acgatagcga atggcaggtt gcgtcattca tttccctact ttctatataa
     7981 aatatcttga caactcccac catcataatc gtttcaagtg ctttttgcaa gttccctcgg
     8041 cttccttcta ctttatttaa tattcttttt tttatccttt ttttatgaat aacaaaaaaa
     8101 agaaaaacat tatgacagaa atattttttg atacatggta tattggtgtt attaaagaaa
     8161 aggagggaaa aatcatgcaa tcttctgcag aattaaaacg ccaagcaaaa gagagtctaa
     8221 aaggacgttg gggacaagct gttttactaa atctaatccc tacattgatt ggtattgcta
     8281 ctattcttct tattatgatt cctattatct tgtttgccac caccatatat gatggatcag
     8341 cgagcgtctc agaagcgaca acttcaggag gcggtagttc aggaggcggt ggcggaatta
     8401 tttccagcat cattagcgct ttgttcatga gcggtatttc ttggacttat cttgatattt
     8461 tacgtggaca aaaagatcag atcgaaccat tcaaagatgc atttcgtgga ttcaaaggtt
     8521 tgtttatcgg cggagtgatc agcttagctg tgctgatctc gatctttaca acattatggg
     8581 gtttgctatt agttattcct ggaatcatta aaagctattc ttattctcaa agctatttta
     8641 tttactacga tatcgtccaa gaaacaggac aaacacctaa agtactggat acgatcactg
     8701 caagtcgaaa attgatggat ggttataaag gacaactttt ctggttagat ctttcattta
     8761 tcggctggca tatcttagca ttagcaactg ctggtattgg ttatctatgg ctcagcccat
     8821 acatttctgc aacaaaagca gcattttatg aacaattgcc aaaagatatt taaaagagag
     8881 tgtgacttgt gaccgagggg gtggcaggag aacaccgttt atcgagaggc agtaaaagaa
     8941 acattcagcc cattagcaaa aaggaggtgt gacaacattc atgtcacacc tcctttttgt
     9001 atagttccag taaaaagaaa atcaacttag aaacagttaa tcattcctct tcttctgctt
     9061 ccggtaatcg atggaacttg atcagttcat tcaccgcgat tggtacgata gataaaatcc
     9121 ctaccaagaa ccagtgattc aaactgatgt gtactaggcc gaatagttct tgcgtaaatg
     9181 gcagcaaagc aacagccgtt gtaatagcta atgccagtaa cgccatttct accaaagctt
     9241 tattggtcgc taaattgact ttgaagatcg attggctgct gcgtacattg aacacatgca
     9301 gaatcgatga ataagctaag atcaagaatg caacagtttg accgacatgc tgacttgcgt
     9361 ctacccatgg gctgatagta tcgacataac tacctaaata ataaccaaac aaggttacga
     9421 tggtgaagac ccctgcattt atggcaattt tttgcaataa acctcgagca aagatccctt
     9481 catttttcgg aatgggtggc tgatccatga tgccgtgttc cgctggctct cttcctaaag
     9541 caaatcctgg aagtccatca gcaactacat tgacaaacag tagttgcaca gctgtaaatg
     9601 gtgctcccca tcctaacagc atcgcaatca aaacaataaa gatctccgaa atattacagc
     9661 ttagcaaaaa gttgatagct ttacgaatat tctgataaac cgaacgtcct tgactgacag
     9721 cgtctacgat cgtcgcaaag ttgtcatccg tcaaaatcat atctgcggca ctttgcgcca
     9781 catctgttcc tgtgatcccc atggcacacc caacgttact tgctttcaac gctggtgcat
     9841 cattcacacc atccccagtc atcgctacga ctgcacccgt acgttgccaa gatttgacga
     9901 tccggatctt gtcttctggc gttacccgag catagacaga gagagctttg acacgcgcat
     9961 ctaattcttc gtctgataat tgatgcagtt ctgatcctga aagtgcttct gacggatcat
    10021 tcaagatacc taattctttc gctatggcac tggcagtcac cacatgatct ccagtgatca
    10081 tgactgtttt gatgccagcc tttttcgctc gcttgattgc tcctttactt tctggacgtg
    10141 gtggatcgat cattccgatc agaccaatca aacgtaagtc tttttccagt gcttcagaag
    10201 aaatatcttg cggttcttcg tcataagtag cataaccaac agcaatcact ctcagtgccc
    10261 gtttaccaaa gcgatcatta acgatcgccg cttgttctac atcgcctgaa ctaaagcgcg
    10321 gagcaagaac atcgaatgct cctttggtga ttgagatgta tttttcgccc atttggtgaa
    10381 ctgttgtcat catttttcgt tcagaatcaa aaggaagctc gtttactcgt ggatattttt
    10441 cttctaattc tgcctttgta tgataattct cttcaacagc ccttacgatg gctacttctg
    10501 ttggattacc tttgataaca aggtcgtcat cttcttgctc gaccgtcaca tctgtacata
    10561 gtgcagccat tttcaatatt tccatcgcgc tgttcgtcat tgaatcttcg atattcgtta
    10621 cttcatcttc tttgctccat acccggcgga cgcgcatttc attttgtgtc agcgttcctg
    10681 ttttgtctga gcagataaca ttagcggttc ctagcgtttc aactgctggc aactgtcgga
    10741 tgatcgcatg tttcctcgcc atcttctgca caccgtatgc aagtgtcaac gtaacaataa
    10801 cagtcaaggt ttccggtaca gctgcaacag ccaaggaaat cgaagtcatg aacatctcaa
    10861 ggagtggttc tccttgcagt tcaccaatca caaatactaa agccgcagcc gctaaagcga
    10921 tcatactgat tcgtttgcct agctgattca atcggacctg taaaggtgtt ttttgcatag
    10981 tattttcgtt caaaagatcc gcgatcttac ccatttctgt agccatgcca atggcagtga
    11041 cgatcgcttt cccacgacct gcagcaaccg tacatccttt aaataccata ttaagttggt
    11101 cacctaatga ctcatcttct tcagcaataa atgctgcatc tttttcaact gcttcacttt
    11161 ctccagtcaa cgccgattct tctacacgta attgtgatgc ttcgatcaaa cgggcatcgg
    11221 cagaaatagc atcaccggac tctaaaacaa gaatatcacc cactaccagt tgctctgact
    11281 caacagtggt cactttgccg tctcgtatga cagttgccgt ttgtttattc atatcttgca
    11341 aggattccag tgctttttct gcgtttcctt cttggacaat cgccaatacc gagttgatca
    11401 caacgatagc gatgatcagc agtccttcaa ataaatcccc atgttctgta gcaaatgcgg
    11461 tataaaagga aatcaccgct gctacaagaa ggatgatcgt tgtaaaatcg gacaagcttc
    11521 gaaagaattt tttgatcatc gattctttcg gcgcctcatc gaatttattc aatccctgct
    11581 tttttcgccg ttcatccact tcttgtgaag acaacccagt atctggatct gcttctgtta
    11641 ccttctttac ttcttctatt gtctgcttgt attcttccat cctattcctc ctttaaaatc
    11701 ttcgtacaaa aaaaggtact tattttacta taacatcctt tttttattta cttcaaataa
    11761 agggcttatc gattatttgt taagaaagcg taaaatttaa agtgcttata tttgcaaacg
    11821 ctaccaaagg atgctattat atagatgtaa ggaaggagga taccatcaaa tagacagaag
    11881 aatttctcga tagaacagtg taagcaaaca aagttgttag aagtacaggt ttcacccaga
    11941 catgcgtgta gagtacaaac ctataaatag tgaatgcgta tggatgtttg gatcacgaag
    12001 tatccgaaac aagaagtaat gcgatacgta acaattgaat aacgtaaacg tctgaaaatt
    12061 gaataggaac aagagatgtt tcaaatgttg aagatgtaac gaaaacaaaa tttttgttta
    12121 acatgaagaa attcaacacc gtgctttgca tgtatgatgg aaagttacaa tcatttctgt
    12181 ttatttgaat ccttccgagg tacaagaaag ccgagcttat cgagagctcg gctttttctt
    12241 tgtcataaaa tctttgtaaa gcttttttgt tattcaaaac tagcggcttt tcataacctt
    12301 atttcacgaa catcgcatct ccaaaactaa agaaacgata gcgctcttca atcgcatgct
    12361 ggtaagcaga taaagtcaat tctcttcctg caaaggcact aactaacatg actaaagtcg
    12421 attttggcaa atggaaatta gttgagaaag cttctaccac tttgaattca tagccaggcg
    12481 taatgaaaat atcagtccat ccgctgtctg ctttgatttc tccattaaat ttcgttccaa
    12541 tcgtttctaa tgttcgaatt gaagttgttc cgacagccac gattttcccg ccatttttac
    12601 gtacttggtt caatcgctca gccgcttctt ccgtcaaacg gtagaattca ctatgcatat
    12661 ggtgttcttc aattttatct acgctgacag gtcggaatgt acctaaccct acatgtagtg
    12721 tcagatagac taattcaaca ccctttgctt cgatctcatc aagtaattct ttcgtaaaat
    12781 gcaatccagc tgttggcgct gcagcagaac cattttcttt cgcatagaca gtttgatagc
    12841 gatctgggtc ttccagacgt tctttgatat atggaggcaa tggcatttct cccaatgatt
    12901 caagaatctc tagaaaaatt ccttcatact caaagtcaat gatccgtccg ccgtgttcca
    12961 attcttcttt cactactgct tgcaatctgc catcaccaaa agagattctt gtcccaactt
    13021 tggcacgttt cgcaggtttg atcaatgtct cccacgtatc tccttgtgta ttcgttagaa
    13081 gaaggacttc taaatgcgca ccagtatctg gtttttctcc atataaacga gcaggcagaa
    13141 ctcgtgtatt attcataact agcgcatcac caggctgtaa ctcttcaatg atttcatgga
    13201 aatgtttatc ttccatctct ccagttttcc gatccaaaac aagtaaacga gaatgatcgc
    13261 ggtcttttaa tggtgtttgt gcgattaatt cttccggcaa gtcaaaatca aaatcttctg
    13321 ttgttaacat atcgtcactc actttctatc atgtgcttca tcattctatc atgtttttcg
    13381 ttctgttggc aaaatcagca ctttttcact tctatttttt gttttcctaa ataaaaaaac
    13441 taccctattt gtattttttc attgttttag gcatactagt aatagatagt tcttcattga
    13501 aaatagaaaa agaggtggca ccatgattga atttcaaaat gtgtcaaaaa tttataaagg
    13561 tggaaaagtc gctgtagagg acgtcaatct ttcatttgaa aagggtgagt tcatttgttt
    13621 tatcggtacg agcggaagcg gaaaaacgac gacgatgcgg atgatcaacc gaatgactga
    13681 tccaacacaa gggaaaattt tgattgatgg aaaatccatc caagaaattg atccagtgga
    13741 attgcgacgt cagatcggct atgttatcca aagtattgga ttgatgcctc acatgacgat
    13801 ccgtgaaaat atcactcttg tccaaaaatt gttgaaagtc agtcaagaag aacggaataa
    13861 aacagctgaa aaaatgattg atcttgtgga attgccccgt gagatgttag atcgctaccc
    13921 acatgaatta tctggtggtc agcagcagcg tattggagtc gttcgtgccc ttgcagcgaa
    13981 tcaagatatc atcttgatgg acgagccatt cggtgcgctt gatccaatca ctcgtgattc
    14041 cttgcaagac cttgtcaaag atttacaaga gcgtttggga aaaacgatcg tatttgtaac
    14101 ccatgacatg gatgaagc
//
LOCUS       DACGLW010000078        13918 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.081, whole genome shotgun sequence.
ACCESSION   DACGLW010000078 DACGLW010000000
VERSION     DACGLW010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13918
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.081"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            132..506
                     /locus_tag="IXT53_002262"
     CDS             132..506
                     /locus_tag="IXT53_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ4906058.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(620..1249)
                     /locus_tag="IXT53_002263"
     CDS             complement(620..1249)
                     /locus_tag="IXT53_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ4906059.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1383..1455)
                     /locus_tag="IXT53_002264"
     tRNA            complement(1383..1455)
                     /locus_tag="IXT53_002264"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1420..1422),aa:Thr,seq:ggt)
     gene            1669..1742
                     /locus_tag="IXT53_002265"
     tRNA            1669..1742
                     /locus_tag="IXT53_002265"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1703..1705,aa:Asn,seq:gtt)
     gene            1697..1846
                     /locus_tag="IXT53_002266"
     CDS             1697..1846
                     /locus_tag="IXT53_002266"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906060.1"
                     /translation="MTVNHDVVGSSPTAGVNKAYAKKKKSNHLGFFFFYYYDESSFLF
                     LSRML"
     gene            2339..3667
                     /locus_tag="IXT53_002267"
     CDS             2339..3667
                     /locus_tag="IXT53_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HAQ4906061.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            3869..4702
                     /locus_tag="IXT53_002268"
     CDS             3869..4702
                     /locus_tag="IXT53_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HAQ4906062.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            4718..5455
                     /locus_tag="IXT53_002269"
     CDS             4718..5455
                     /locus_tag="IXT53_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HAQ4906063.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(5610..6578)
                     /locus_tag="IXT53_002270"
     CDS             complement(5610..6578)
                     /locus_tag="IXT53_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HAQ4906064.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            6810..7643
                     /locus_tag="IXT53_002271"
     CDS             6810..7643
                     /locus_tag="IXT53_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HAQ4906065.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            7677..8516
                     /locus_tag="IXT53_002272"
     CDS             7677..8516
                     /locus_tag="IXT53_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ4906066.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            8542..9102
                     /locus_tag="IXT53_002273"
     CDS             8542..9102
                     /locus_tag="IXT53_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ4906067.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            9301..11022
                     /locus_tag="IXT53_002274"
     CDS             9301..11022
                     /locus_tag="IXT53_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HAQ4906068.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            11187..12329
                     /locus_tag="IXT53_002275"
     CDS             11187..12329
                     /locus_tag="IXT53_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HAQ4906069.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            12345..13556
                     /gene="thiI"
                     /locus_tag="IXT53_002276"
     CDS             12345..13556
                     /gene="thiI"
                     /locus_tag="IXT53_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HAQ4906070.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4751 a         2298 c         2833 g         4036 t
ORIGIN      
        1 tctacactct gagatgtaag aaatatcata agatatttct tacatctttt tgttttgagc
       61 aggactttag ataattttat taatatatgc tagcatgctt ttgtaagaaa aagaaagagg
      121 gcgtattagt tatgtatctt aaaattacgg aacaagcaca agaaagatta caaaaatata
      181 ttgatgaagg cgcaacagtt atattggatt tagatgatgg cgttggggaa tattctaaaa
      241 tgggtgtttg ctctttagat actagttttc gtttacttct tcttgataaa gaacagagaa
      301 aaaatgatta taaactccaa ttagatagtg atattgggga tgtttatata aaagattatt
      361 caaaaatgta catggatgaa catatgaccc tttctttaga tccacgtcta ggtgtattta
      421 ctcttgaaag cccaagtggt tctttagaca gtcatgttca attagtagat ctacgaccag
      481 aaaaattctc tggaataaaa caataacttt ctctctattg tccataaaac tagtctcaat
      541 caagctgtat tgaaaaaaca agacaacatt cttaccatgg taaataaaaa gacgacacat
      601 tttgtcgtct ttttttaaat taactatttt cgataaaaga tcgcatagct tgtgccatta
      661 tttctggctt ttctctattc aattcatgtt ttccgttttt aataaagagt agccggccat
      721 tcgttgaaag tttatataaa cgttttgctg ctggtttatt cgcatgatct ttggtaccgc
      781 ataaaatcaa tttaggaagt cgtgatgtac gaagctgttt tttgcagtct aatgtttcaa
      841 gtgaacgaag tagggtaagg gtttgttttt tagatagacc taatttttca aatatcttag
      901 aaggtacgca gtaaaaataa atattctgga aagtaagcaa taagcgatta ggagactcaa
      961 attgtggagc gactaaaaca acctttttta aatatggaga aggagttgca agatagttta
     1021 aagcaataac agcgcctaaa gataatccac ataaaataaa aggttccttt atctgattca
     1081 attgtacttc gatgtatttg gttaagtcgt ctaaagtagc agtgtctgtg ctgtttaaat
     1141 gattgaacaa tgaaagactt ttgcaagaat aactattggg tagataagag attacatctt
     1201 tccatgattg ttcgtcttgg cctagaccat gcaaaaaaac aattttcata ttgctctcct
     1261 ttttatagtt atcatataga ctttctttgt actattaaaa tataaacatt catttcttac
     1321 gtcataaaat aaaaaaacag cgcagacaca ttgttaaacc aacatgttgc actgtttaag
     1381 gaagccactt gccgggcttg aaccggcgac ctcttcctta ccatggaagc gctctaccag
     1441 ctgagctaaa gcggcattat cattaataca aataatagga taacgaatat taatgtattt
     1501 gtcaacagaa ttttttcgaa aaaatgtgtt tctctaatag gaaaacagat tttagaaaaa
     1561 ataaaaataa caaaaaatgc gaacggtttc tttgataata taataaaaaa attcaaaaaa
     1621 agtattgcgt tatttagaag aaattgatat tatattcatg tacttgattc cggcatagct
     1681 cagttggtag tagcgcatga ctgttaatca tgatgtcgta ggttcgagtc ctactgccgg
     1741 agtaaataaa gcttatgcaa agaaaaagaa atccaatcac ttgggtttct ttttctttta
     1801 ttattatgat gaatcaagct ttctgttttt gtcacgtatg ttgtaatctt tacgaaagag
     1861 tagagttggc tactcataat caaccatact tcccatcatc gctaaatacg aaataaatca
     1921 gcattcctac accaatcaaa gcacaagctg caccaagaaa agggatgaca ttcccaaagt
     1981 tatctgtatt cataagtatg ccaccaccca caaacaaaaa gccgccagca atcaggtttt
     2041 tcattttatt catctccttt cttacgttat agtatttcct tctttcaatg agtattttga
     2101 aagctatctt attgtatgat gttcttctag gcctcttctt tattatacag gccattttcg
     2161 ttatctcata gaaatattcc tttttatctg aaaactattg tataccgtat gacatttttc
     2221 ttcatgaagt tgtaagtata ttctcattca aatgaaaatt tcatcataga agagattgta
     2281 tttctaaatt cttaagagta aaatacgaag tataacttgg atttggaagg atgattggat
     2341 gaaagctatt acgcctgata tgacaacaaa atttaacaaa cagtttcaag caaatatcaa
     2401 acagcaggca ttgcagcggg cagttgtgaa gaacggaatt actgcttctg ctgaaaatgt
     2461 ttcagcacat gtggagaata caccggtatt ttctatagat ttggctacgg ggaaagtggc
     2521 taatcaaatg caatcggggc gttgctggat gtttgctgca ctgaacacat tccgtcataa
     2581 gatgctgaat caattccatt taaaagagtt tgaattatca caaaattaca cttttttctg
     2641 ggataaatat gaaaaggcaa attatttcta tgaaaacatc attgcaacag gaaaagaggc
     2701 aatcgatagc cggaaagtag ctttcttact cgctactcct caacaagatg gcggccaatg
     2761 ggatatgatc gtttcattat tccaaaaata tggcgttgtg cctaaatcgg tgatgccgga
     2821 aagtgcgaat agttcaaatt ctcgagattt gaataactac ttgaacaaat tactgcgtaa
     2881 agatgcagta gtgttacgga aaatggttgc tcaaggagaa acattagacg ctattgaaga
     2941 aaagaaagaa gaaatgctag aaagcattta taacttctta gctatctcat taggaacacc
     3001 tccaaaagaa ttcgattttg agtaccgcga tgaagaaaag aactatcatc ttgaccgagg
     3061 actgacacca caaatttttt atgataaata catcggtgta aaattagatg attatgttag
     3121 cgtgatcaat gcaccaacaa aagacaaacc attcaatcgt tcttatacag tagagatgct
     3181 aggaaatgtc gttggcggga aagaagtcaa atatctgaat gttgatatgg aaactttcaa
     3241 aaaattagcg attgctcagc ttgaagaagg agaatctgtt tggtttggtt gtgatgtagg
     3301 tcaatcttct actagaacta gtggcatcat ggccttagat gcttattcaa tggatgatct
     3361 atttgatacg gattttacaa tgactaaagc tgaacgttta gattttggag aaagtctgat
     3421 gacccatgcg atggtattga ccggtgttga cttagtagac gggcaatcaa caaaatggaa
     3481 agtcgaaaat agctggggcg aaaaagttgg aaagaatggc ttctttgtta tgagtgatgc
     3541 ctggatggac gaatatacgt accaaatcgt tgtaagaaaa gatttattga cgaaagagca
     3601 attaaatgcg ttcaatgaag aaccaattgt tctatctccg tgggatccaa tgggtgcttt
     3661 agcatagacg agtatttcaa tgaagagtat gtagtaaaga taaaaagagc tggcttaagc
     3721 cagctctttt tatctttaaa gtccattccc tgttttttat ggtccctgtg ctataattct
     3781 aaataaagga taaggattgg aaataatcct tttatcatta aaaatatgat gacgaagtag
     3841 aataaaattg ggagtagggg ggaaagtcat ggatatccaa actgttttat ctgatctgaa
     3901 attaaatggt aaagtgattc cagttgttgg tggcgatgtt aatcagacct atcgtataaa
     3961 aaccgaacat cgtgcttatt ttttaaaaat ccatccgaat gtaaaaaaag gattttttga
     4021 agcagaagta gacggattaa aagaactgtc agcattcgta cgtgtaccgg atacgtatat
     4081 gctaggtgaa acaagtgaag gcgcttattt gttaatggaa tggatcgagc cgggaaaagg
     4141 ggatcaacga gatcttgctg cagctttagc taatctccat cagcaaacag cgccacaatt
     4201 tggttttcgt aaagataact atttgggtac acttgttcag aaaaattctt ttgaagaaga
     4261 ttggtggact tttttcttta aagatcggct tgaatcccag atttctttgg cagaagaaac
     4321 gaatcgatgg aatgtacagc gtcaagaaaa gtatctgcga tttaaagaac gtgtattgaa
     4381 atcggttgaa ccaaaaaaga tcactccacg cctcttgcac ggagacctgt ggagtggtaa
     4441 tgtgttcttt gaccagcaag ggcatccagt ttttgtcgac ccagctgttt cttatgggaa
     4501 tcgggaacaa gatattgcaa tgagccagtt attcggtggt ttccgaccgg aatttttaga
     4561 tgcttatcaa actatatttc cattagaaaa gggatggaaa gaccgactgc cgatttacca
     4621 gttatattat cttttggcac atttgaatat gtttggagaa agttacggtt cccaagtaga
     4681 tcaattacta gaaaattttt aagaggaggg cgtgtttttg gcaaaaaaat ataccaaaaa
     4741 gcatgaagtt tcatattatg agtgtgacat caaccaaacg atgacatttc cttcgatgtt
     4801 aggagtagtt atcaaaacgt ctgaagatca aagcgaccag ttgggaagag gcagtgaatt
     4861 cgtcaatagc tttggactta cttgggtcat cacgaattat tccatggaga ttaatcgtct
     4921 ccctagagtc ggtgaagtaa tctcagtaac gactcaagcc atggagtaca ataagttttt
     4981 ctgttatcgg aacttctgga tccatgatga agatgggaaa gaattagtca aaatagaatc
     5041 tgttttcgta ttgatggatt ttgtgaatcg aaaaatgagc agtgttaatg aagagatcat
     5101 tgctcctttt gagagtgaga agatcaagaa aatcaagcgt caggaaaaaa ttgaaaagat
     5161 tgattcgggc ttcatgttgc catatcgtgt tcgtttttat gatatcgata gtaatcagca
     5221 tgttaataat gcgatgtatt ttaattggat cattgacgta ttaggatatg actttttgac
     5281 tacgcatgtt ccagaaaaag taattattcg atttgataaa gaagtagagt acgggaatga
     5341 gatcgaaagc cattttgaac aaatccctat cgaaaatggt gtcaaaacaa aacatgaaat
     5401 ccgaatgcaa gaacagctgt attgtgaagc aaatattata tggaaaaata aataaaaaag
     5461 aggttgtgac agaagggggc aacttcaaga aataagaaga aatttacgaa aattgtttct
     5521 caaatttttg taaattttcg acttagtttt cgaagaagct atttttgtca cagcctctct
     5581 ttttaagttt agctattaga taatgatgtt tagttttgga taaacgaaat caattcttca
     5641 cccgacaaac catagtttac agcaatcaat agttttaaac gtgctttttg actattaata
     5701 ctgttacaga aaataatccc cattttttct agttcttttc ccccgccttc ataatcataa
     5761 acaggctctg cgattccatt gaaacaacga gaaacaagta ctacagggat tttttggaca
     5821 agaagctttt ccaaagcagc taaggcttgt ggagggagat tccccgcacc taaagcttcg
     5881 attaccaaac cgtctacttt cgtatgggcg atggcttcca ataagtcccc ttgcatccca
     5941 gcatatgctt taacgatcgg gatagtccca tctacagcgg aaatgtccag tcgctttgtt
     6001 tctaaaagtt cctgcaagaa taaaattcga ttcttagtta ctaagccgat gggacccaat
     6061 gtaggtgttc ggaaagtagc aacatttgtg gtatgggtct ttgtcacgta gcgtgcagaa
     6121 tggatctcat cgttcatgac caccaaaacc cccttatcta gcgcttcttc acaacttgcg
     6181 acacgaatcg cactttcaaa attataaagg ccgtcacttc caagttcatt acttgaacgc
     6241 attgccccag tcaaaacaat tggtaatttg ttgccaatcg ttgtatcaag aaaaaaagca
     6301 gtttcttcta atgtatctgt accgtgtgtg atcaccactc cggatatacc tgatgcataa
     6361 gcggacaaga tacgttcttt taataccaac atgtgagaag gcatgatgtg tggtgaggga
     6421 agattgaaga tatcttcaac taataattct acatgcttgg ggattgtgat ttctgcatct
     6481 aataatgggt ttgccacatc aggactaaca ccgcccgtga gcatgttttc tttcatggcg
     6541 atcgttccgc cggtgtgtag tacaagtatt tttttcattt tacagacttc cttacgaata
     6601 attttgcata gccgttaaat cttattgtat actatataaa agagttttgc tatcagtgaa
     6661 gaataacgaa tagcctaact tatcataaaa aagctgacct tattaaaatg gaaaagactc
     6721 aaccttgacc tcctaataca atggagtatc tggttgagtt tcttttacaa gaaagcagcc
     6781 aaggaaatca atcacgaagg tggccggaaa tgatcagaat cggattaaca tcgtttaaag
     6841 aacatgacaa attaactggt aaaaagaata acactcttta tgaatatgca gggtttctac
     6901 ccttagtgga attagatact gcctattatg gaattccccg aaagtcctca gttgagaatt
     6961 ggataagtga agtacctgaa tcatttcgct ttgtagtcaa agtctatcaa ggaatcagcg
     7021 gtcaaggaga ctggaagcct tattatcaga ccgaagaaga gatgattgcg cattttttag
     7081 atgttatgtc gccaatggta gatgctggaa agatgttttg ctttttagtc caattcccct
     7141 catcattcaa atgtacgaaa gaaaatgtcc agtacttgcg caaacttaga aagtggtttg
     7201 agggttatcc tgtttctata gaattgcggg attattcttg gtacagcgat gaatttcttg
     7261 aacgtacaag aaactttatg tctagagccg ctttttcttt agccatcatc gatgagccac
     7321 agttgctgaa tacaacgatt ccttttgatc catttgtcac caatccgaac tttgctcttt
     7381 ttcgattcca tggtaggaat gcgcaaggat ggcaagcaaa tgataaagag tggcgtaaaa
     7441 aaagaacgtt atatcgttat tcagaaaaag aactgcaaga actagcacat gaagtcaaag
     7501 tagttgattc taagacaaaa gaggtaggtg tgatttttaa taataattct ggtggtgatg
     7561 cggctgaaaa tgcattagca ctgcaaaaaa tattaaatat caagccagat ggactcaatc
     7621 caacccaaat ggatctgttt taagggctta ttttattcaa gaaaggggcg aagtctatga
     7681 tcaaagctat tttttttgat attgacggaa cattggtcac caatcgttca aaagcgttgg
     7741 aatcaacaaa acaagcaatt gaatatgctc ggaaaaacgg cattttatgc ggtgtagcaa
     7801 cggggcgcag cccagtaaag atcaaagaaa tcattgatga attggaatta gatatgtatg
     7861 tcgtgtataa tggtcaactg gtttttactg cagatcgcac gattatcgac cacccttttg
     7921 aacaaaaagt attggaacat attgttgaat ttgcagatga gaaccatcga caaatcgttt
     7981 ttggtgcgag aaatcggtta gatggatcaa cgacgatgct attaggacaa tcgattttta
     8041 tcaagcgact agtcagcttt ttaccgcgta aattcccggt acgtctgatg aaaaaaatcc
     8101 tccaggtttt tagccctcac cgacaaaagg atcgttatga aacgttagag atattaaaag
     8161 aaccaattta tcaatgtata ttgctcagtc cggagtcaga acagaaaaag ttagaacatc
     8221 gctttcccga gtgtacgttt cagcgttcaa atacgtatac tgtagatatc atcccaaaag
     8281 gtggttcaaa attactaggc atccaagctt tcgcaaatgc tgtcgaaatt gaaatggaag
     8341 aaattatggc attcggcgat cattacaatg atatcgagat gctgaaaggt gtaggaattg
     8401 gagtggccat ggggaatgct cagatcgaag taaaacatgc tgcagacttt gtgactcaat
     8461 caaatgaaga agatgggata tacgaaggac tgaaacactt cggtctgatt tattagtcaa
     8521 tgaaagaagg aaaaaatcag tatggaagca gagacacctt ttcaaaaagt agaaacattc
     8581 cacaaaactt ttgatccaag aagaccagag atacccacgg cttttacaga aaatgaagct
     8641 catacacgtg ctgcgttcaa aatagaagaa cttgttgaat ttgttgcagc tacttcacct
     8701 aataaagaac aactgcatga agctgttatg ctgctgcacg aagcattaga taaagcagaa
     8761 gaaaaaatac ttggtaaaac acagtgggga agtgatccgc tggttgaaca agtagatgca
     8821 ctgacagata tcctttactt tacttatggt tctttttcat tactaggaat agtgcctgat
     8881 cgaatattcg aaatcgtcca tcaggcaaat atggggaaat tatttccaga tggaaaacct
     8941 cattatgacc ctgtaacaaa taaagtgttg aaacctgaaa actggcagat ggaatatgct
     9001 ccagaaccta aaatcgcggc agaaatccaa cgtcagattc aagaagcgat tgctaagaag
     9061 aaatccgctg atataggcat gtgcacaaat gaaaaagagt gatcagagtt tcactatttt
     9121 tgttatcttt atacgatgga aaaacaaaag attttcgttt aagttagtga atgcatgaaa
     9181 atagtatgct ataatctaga atagatgtct tttgcacgag agacgtatta tttggattta
     9241 tgccaagcag aacgccatct gttgcataaa agatgcatac tgaaaacgga ggaaccataa
     9301 atgggaaata atgttatcat cggaatcgtc attgcaatca ttatagtcgc cattgtcgtc
     9361 tttttggtta gttacttgat gaagaaaaaa aaccaagatc gattggatgc cttagaaaaa
     9421 cgcaaggaag aattatttga cttgccagtg attgaagaag tcgatgatgt taaaaaaatg
     9481 catctggttg ggcaaagcca aaataccttt cgtgaatgga gtcaaaaatg gaatgacata
     9541 tctacttcct cgttcgctga actagaaagt caaattttcg aagtggaaaa cctcaatgaa
     9601 agttttcggt tcttaaaagg aaaaaaagca gttgttgaag cagaagctac aatggaccaa
     9661 atggagaaac aagtgacgga aatccgtgaa ggtctaaagg aattgcggga aacggaagaa
     9721 cgcaactcac tagctgttca gcaggcactt gacgtctatg aagaactgaa agaaaaagtc
     9781 agagaagacg agcatgaata cggaccagct ctttttgaat tgcaaaaaca gatcaaagtg
     9841 atcgaaaaag aatttacgca gtttgtgact ttaaacactt ctggagaccc tgttgaagcc
     9901 cgcgaggttt tggataaagc agaaaaacat acgtatgaag tggaagctct gatgaaaaaa
     9961 attccaccgc tgtatgagga gctccatacc actttccctg aacaacttga agaaatcagt
    10021 gatacttacg agaaattgat ggaagaacaa tatgtattcc cagaagaaga tcttgctgaa
    10081 gatatcgcaa aagtaagtcg aagaattgaa aattcattag ccaatcttga aaaaacagaa
    10141 gttgaaacgg tcgaatttga aaaccgtgaa acagctgatt tgattgattc gttatatgat
    10201 atcttagaac gggaaatgga agctcagaga tatgtaaaaa ctaaccaatc aacgattgca
    10261 gagtatatca aacatacaac aaagaataac cgtcaattat tgattgagct ggatcacaca
    10321 tctcaaagct atacattgaa ccacaatgaa attggccgtg tacgaggttt ccaaaccgaa
    10381 gtggaagaaa tggaacgtca aaatgaacag atgattccac agatccgtca acacgaaatt
    10441 ccttattcag aagtgcgcac gttctataaa acagtcttca aagtcttaga agatatcgaa
    10501 aagcaacaag tagagatcga cgactcatta catgaattga gaaaaggcga aaaagaagcg
    10561 caagaaaaga tcgatacatt cgaattcaaa ttgcgcagtt tgaaacgttt tgtagaaaaa
    10621 caacgtttgc ctggcttgcc gaatgattac ctagaattct tttttgttgc tactgaccgt
    10681 attgaagaac taagtgttgt cttgaataaa attcgagtaa acatggaaga ggtcaatcgt
    10741 ttagttgctc tatgtgaaga agatctagaa ttattagata agaagacaca tgatttagta
    10801 gatgcagcag cattaacgga gcaaatgctc caatatgcaa atcgttatcg tcatacacac
    10861 gaagaagtga gagaagcaat tgataaaagc tactatttat tcaataaaga atatcactat
    10921 caagaagcat tagacgaaat cggtacagca ttagagcgag tagaacctgg tgctttcaaa
    10981 cgcatcgaag atttttattt taatcatcca gacttagtat aaagcaaaaa agttagtaaa
    11041 ccagcaagag caagacggat tattgtcttg ttcttgtctt tttttgtata tttgaaaatt
    11101 gaaacaagtg aaaatttcac ctgctcttga aagcaccttg gacaaccgtt ataatagaaa
    11161 agaatttcaa aaaagagggg tttcttatga tatattttga taacagtgca acaaccccaa
    11221 tctttccgca aagcttagat gcatatgtaa aaaccagtca gcggattatt gggaatccat
    11281 caagcttgca tgatttaggc aatcaagcta accggttgtt gcagcaagca agaaagcaga
    11341 tcgctgattt gatccatgta gaaccagaag aaatctattt tacttcaggc ggaacagaag
    11401 gagataactg ggtattaaaa ggaacgatga tcgaaaaaag agaatatggg aatcatatga
    11461 tcatttcagg agtcgaacat ccggccgtca gcgagacagc agaacaatta aaagaattag
    11521 gaatcgaagt aagtatcgcc ccagtggata aacgcggatt tgtacgtgtc gacgaattga
    11581 aagaattaat aagaaaagag acagtattag tctctgttat ggctgtgaat aatgaagtag
    11641 gatcgattca gcccatccag gaaatcagtg atcttttgga tgattttcct aaaattcatt
    11701 tccatgtaga tgctgttcag gcgattggaa aagtaccatt ctcgcaatgg ttgacagaaa
    11761 gagttgattt tgcaacattt tcagctcata aattccatgg cccacgggga actggtttca
    11821 tttattggaa aaagggtcgt cgtttagcac cattactgac aggtggaggg caagaaagaa
    11881 atcagcgaag tgggacagaa aatgttccag gtattgtggc tatggttaaa gctttacgtt
    11941 tatcattaga gataaaggaa caaaaacctg agcagacaca acgcttaaaa gtagctctaa
    12001 tcaatgcgtt gaagacgtat gagaaagtga cgattttttc agaaggaact gactatgcac
    12061 cgcatatttt atgttttgct ctaaaaggaa ttcgcggcga agttctcgtc catgccttag
    12121 aagaaaaaca aatatttgtt tctactacta gtgcctgctc ctcacgtaaa catatggcaa
    12181 gcagtacatt gcacgcaatg catgtaccaa atgatctggc tacatctgct gttcgaatca
    12241 gcttagatga aaacaattcg cttgcagaag tcgagcaatt tatgattatt ttcaagcagc
    12301 tttataagaa attttctgca atcaattaaa agaggagtgt tcttgtgaaa tacagtgaaa
    12361 ttatggtccg ttatggggaa ctttcgacaa aaggaaagaa tcgaaaaagc tttatcatgc
    12421 aactagcaca aaatgtcaga caagctttga aagactttcc agaaatcaaa atccatgctg
    12481 atcgggaccg gatgcatctt ttattgaatg gcgcagacgg gcagctagtt attccaaaac
    12541 tagcaaaaat atttgggatc caaaatttct ctccaagtat tcgtgtagaa aaagatgtcg
    12601 aagttttgaa acaagctgtt caagacatca tgaaagaaat ttatactggg aaagaaacat
    12661 ttaaaattat cgctaaaaga agcgatcacc aatttgaatt gacaagtaat gaattgaatc
    12721 aaacattagg taatgctgtt tttgatattt tcccgcatat ccaagttcaa atgcgtcaac
    12781 cagatattcc attacgtgta gagattcggc gtgatggcgc ttatctatcg tatgaaacga
    12841 tcaagggagc tggaggattg cccgtaggaa caagcggaaa aggaatgctt atgctttctg
    12901 gggggattga ttctcctgta gcagggtact ttgccatgaa gcggggagtg gaaattgaag
    12961 ctgttcattt tgctagtccg ccgtatacaa gtgaacaagc actgcaaaaa gcaaaagatc
    13021 taacagccaa actagcacct tatgtgggaa cgatccattt tatcgaagtg ccatttaccg
    13081 agattcaaga agaaatcaaa aagtcagtgc cacaaggata ttggatgaca attactcgtc
    13141 gaatgatgtt gcgtttgacc gatgaaattc gccgtattcg tcatggttta gtcattttga
    13201 atggtgaatc attaggtcaa gtggcttcac aaactttgca aagtatggtg gctatcaatg
    13261 aagtaacgaa tacgccaatc attcggcctg tagcgacaat ggataaactt gaaatcattg
    13321 aagtagcaga acagatcgat acatttaatc tttctatcca gccttttgaa gattgctgta
    13381 cgatttttgc tcctccacag ccaaaaacac gccctcaact ggaaaaggtc catcaatatg
    13441 aagagcgttt agcaatagat gaaatgatcc aacgagcact agctggcttg aagatggaag
    13501 agatacaagc agaatcgtct aatgaaaaaa ctgaagaatt ttcagaattg ctataagagg
    13561 ttatgaaaat agtgttttga ccagagtatt agagaaacaa aacgaaaaaa tagctccatg
    13621 tattttaccg tttttgaact aatgtcgaag attagctgat tgaataccat ttatcaagaa
    13681 gttatggaaa agccggttct tttttactaa ctgaaaaata aaattttatt tagagatcaa
    13741 gctacagttg ataaatgata taaaaaagag aatttcccat gaaatagcga ggttgtgaca
    13801 gaagtgggca gcttcaagaa ataagaagga atttacgaaa attgcttttc aaatttttgt
    13861 gaaatttcgg cttatttttc gaagaagtta cttctggaac accgtgtatc agaggttg
//
LOCUS       DACGLW010000079        13820 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.082, whole genome shotgun sequence.
ACCESSION   DACGLW010000079 DACGLW010000000
VERSION     DACGLW010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13820)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13820)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13820
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.082"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            complement(107..472)
                     /locus_tag="IXT53_002277"
     CDS             complement(107..472)
                     /locus_tag="IXT53_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HAQ4906071.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(619..1392)
                     /locus_tag="IXT53_002278"
     CDS             complement(619..1392)
                     /locus_tag="IXT53_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ4906072.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            complement(1566..2426)
                     /locus_tag="IXT53_002279"
     CDS             complement(1566..2426)
                     /locus_tag="IXT53_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HAQ4906073.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            complement(2426..3844)
                     /locus_tag="IXT53_002280"
     CDS             complement(2426..3844)
                     /locus_tag="IXT53_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ4906074.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGKSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            complement(3901..4350)
                     /locus_tag="IXT53_002281"
     CDS             complement(3901..4350)
                     /locus_tag="IXT53_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HAQ4906075.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            complement(4795..5058)
                     /locus_tag="IXT53_002282"
     CDS             complement(4795..5058)
                     /locus_tag="IXT53_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HAQ4906076.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            complement(5157..6452)
                     /locus_tag="IXT53_002283"
     CDS             complement(5157..6452)
                     /locus_tag="IXT53_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HAQ4906077.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            complement(6449..7720)
                     /locus_tag="IXT53_002284"
     CDS             complement(6449..7720)
                     /locus_tag="IXT53_002284"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HAQ4906078.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            complement(7885..8754)
                     /locus_tag="IXT53_002285"
     CDS             complement(7885..8754)
                     /locus_tag="IXT53_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HAQ4906079.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            complement(9046..10656)
                     /locus_tag="IXT53_002286"
     CDS             complement(9046..10656)
                     /locus_tag="IXT53_002286"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HAQ4906080.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            complement(11849..13189)
                     /gene="glnA"
                     /locus_tag="IXT53_002287"
     CDS             complement(11849..13189)
                     /gene="glnA"
                     /locus_tag="IXT53_002287"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HAQ4906081.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            complement(13217..13609)
                     /locus_tag="IXT53_002288"
     CDS             complement(13217..13609)
                     /locus_tag="IXT53_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HAQ4906082.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
BASE COUNT         3906 a         3015 c         2346 g         4553 t
ORIGIN      
        1 aatctgagcg gttttttgag taaaaatagt ttatgattat tcttaattaa acatctattt
       61 atttttaaag ctttcattta tcgataagaa tagtttaact gttgatttat tccattttcc
      121 gttcatctgg tacgatgcct tgacttagca accacttgtg gaagaaccat tctgccacac
      181 cgatcaatag agcaggaatc aatactttca tccaaatatc gactgtatca ttcataaaat
      241 atagatagag ccacaagata actgcagctg aaccagcatc tgcgatcgtc gctgcgatgt
      301 tccctacttt acgtaagatc aacagatccc ctagcacata gatcatgact acaataatta
      361 ccccaacaat cgttgaatcc ataaagctaa caccgtataa acctgtcagc acgacccaca
      421 gaatcgccca gatcatgatt gccttcacta ccaacgcctt cacatgtttc attttctgca
      481 ctcctttctc ctggataaaa aagtaagatt atctaacaag ataacctttt cattattata
      541 ccatcggctg agagaagcga gcaaagataa ggtcctatct aaaaatgagg aagccactta
      601 gaacattcac gtaataattt atccgtacag acattcgata tggtgttcct ttactttcgc
      661 atactgttct tttgtcagtt ctttacttgt caccagataa tcgatttcat gaaaagcaat
      721 ggcttgatgc atgaatttat gtgcaatttt ttgctgtgta actaaaacgg caagccggtc
      781 tgattgtttt ctaaaagctt ttttgaccat tgcctctcct tcatgagttt ctgttactcc
      841 ttggctgaaa tctagcccgc gacaactcat tatcgctaaa tccgcataat atgacaatgc
      901 atcgttatag gcttttgaac cgttgattgt tgctcgtttc ggaacaattg cccctcccaa
      961 tacagatact tttgccgttg tatattcact caacatagca gctgtgacga tgccattcgt
     1021 aatgatctgc aaaccatcaa atcttgctag gtttctgact aagtgtaaag aggttgtact
     1081 ggaatccaag aataagacca tatcatctcg aatcaaagaa gaagctgtat ctgcaatctt
     1141 acgtttttct gtttcttctt ctaatttggt gatcttcatc aggtaaggca cgtcaatttt
     1201 tcgattgtca ataagcatgg cgccaccgcg aactctacgg atcaatccac ttttttccag
     1261 aaaaacaagg tcacgtttaa tcgtggcttc gctataattt actaactcga tcaaatcggt
     1321 gactcttgca aattggttgt tttttagata attcacaatc tcctgctgtc tattctccac
     1381 ttcattcaac ataaaatcac cccatttact ttatatagta acaggaaaaa agaggttgtg
     1441 acagaagtga ttggcttcga gaaataagaa gaaatttttc gaaaaaacta cttctgaaca
     1501 tcgtgtgtgg ttaagagggt atgacaagac tattgtcata ccctcttgac cacacatttt
     1561 tatttttagt ataaactagc cttgcctact gtgttcaaaa tttccatttt atgtttgacc
     1621 acttcttgta cctttttatt agctgctgga taaattgcat tcggctcgta catcgatgga
     1681 ttttcactca atatttctct caaagcattg aaaaagacgc ttttaagatc tgagctcaaa
     1741 ttcactttgg caactccgta ttttactgct tcgctgactt ctgcgtccgg attacctgaa
     1801 ccgccatgta aaacaaatgg aagatcgatt tgttggttca attctttcag taagtcgata
     1861 tttaattttg gagttctgtt tttcggatac aagccatgcg ctgttccaat tgctacagca
     1921 agtgtatcga ttcctgtttc tttcacaaaa atcttcgctt gatcaggatc tgtataaatg
     1981 attgtttcag cacctccttc agcagaacca ttattcccaa tcgttccaag ctcagcctcc
     2041 actgagacat ttaccgcatg agctaacgca accacttttt tcgtaatctg tacattgtcg
     2101 tcgaatgatt tcaaagacgc atcgatcatc acggaagaat agccgtttcg aatcgcccgt
     2161 aagacatctt ctatcgtacc gccatgatcc aagtggataa cgatcggcag tttacttgta
     2221 taagcatagg ctcttactgc cgcaacaaac tcatcactta aataggcgat ttcatcagga
     2281 tgaatttcta agattactgg tgcttctaat ctctctgctt cttcgatgat tgctttcatc
     2341 atatcatagc tgcaaatatt gaaggctggt actgcaaatt gattctcttt agccactttc
     2401 aataactctt tcattgtata taacattacg cttccacctc atttgttaga ttttcatttt
     2461 tcgtccactt gtcttgtttt ttctctttca ctgttttttt caatagtcca agcataaagg
     2521 ctgtagtgac tgtacctgat gcaattgcca agacatacat aggtaaattg ttcataacat
     2581 ttggaattag catcacccaa atccctccat gaggtgccat tgaagtaact cctactgcca
     2641 tggaaatccc tgcagcgact gctgaacctg tcatcaaact aggtatcacg cggatcgggt
     2701 cagataccgc aaacggtata gcaccttctg taatatacga agcgcctaaa atccagcatg
     2761 atttacctga agtccgttct tctttggtaa atttatgttt gaataataaa gtggctaaag
     2821 caatacctag gggaggtgtc attcctgcag ccatacaagc agcaatcggt gcatttactc
     2881 cagctgacat caaaccgatc gagaaagtcg aagtcgattt attgatcggc ccacccatat
     2941 cagctgccat catgatcccg ataagtaatc cgaacaatag cccggatgta tttcccaagc
     3001 tattcagcca atttgtcaaa gcatccaaca cccatgtgat cggttgttca atcacaaata
     3061 ccatcaaaag cccaataatt aagatcgata aaaaggggac gacaatcagc tgataaatag
     3121 cagaaaattc tttttttacg ctaattttat ttgccagaaa atcacagata taccctgcta
     3181 gcacgccacc aatcattccg ccaagaaaac ctgaaccacc tgtagaggcc agtaatccgg
     3241 caacaattcc tggggcaaaa cctgcttttc cagcaatcga tgtagcaatc cccgcgccta
     3301 acgcaggaac catcatggca aacgctgtat ctcctccgat ccttgaaaat gcagctgcta
     3361 acacattata gtctgctgag ttgggatcag ccgctgtaat cccaaaggca aaactcagtg
     3421 cgatgatgat tccgcctgcg atcacaaatg gaagcatgta attcacacca ttcatgaaat
     3481 gattatagat tgtcggcttt ttctcatttg cagctggttc tttttcatta gacacaacga
     3541 atacgccaat atctctcaat gcttcgtcta ttactacttg cggttctttg attgctcggc
     3601 ttgtagagac acgctttact tttttcccgg caaatcggtc caattcgatt tctttatcta
     3661 ctgcaagaat gattgcatct gcctctttga tttcttgttc agataactga ttttctgttt
     3721 ttacaccttg tgtttcgaat tttgcttgat ggtttagatt tcttgctgtt acttctaatt
     3781 tttctgccga catataagta tgagcaattc cagtcgggca agcagttaca ccaacgattt
     3841 tcatgattat tttcctcctt tttcttcact atcgatactc ggacaaagac tctactattt
     3901 tcattcttga ctaatctgtc tgatgatatc tgctggcgta gtggcttctt ttattccctt
     3961 tctaaaatct gcattcatca atcgtctgga gagtgtgctg agaatctcta gatgcatatc
     4021 tgctgatttt tcaggaacta cgatggcaaa aatatattca attggcattt catctatccc
     4081 gctatagtcc gttaatttgg ctgtatgaaa aaaacacagt gctggttttg tcacatattt
     4141 gctttttgca tggggaatcc caaatccatc ttctattcct gtggagatct cctcctctcg
     4201 tttgaatagt gccttaacga ctttttgaac atttcttacc ttatttgttt ttttcaacac
     4261 atcccctatt cgctcaaata atgatccttt tgaatctatc gatggatcga aaacaatcag
     4321 ttcttctgcg atcaattgat caaacctcat aaaaaacctc tcccccacaa aaaatgttaa
     4381 cgttttcttg gaactgaggt cagtatagca tcgctagctg taccgttcaa tattatgaat
     4441 gaacagagga aaaagaccca tcatccgcgt gtataagtca ttaaaatgaa cgatctagct
     4501 cacaaacaat cgcaagtcta taacaaaaca aaaagccttc cttgaaagga aagcttagct
     4561 tctaatttta ggtaaatggt tttttacttc gaaataaccc ctcttcattt cgttaatata
     4621 aggtgttttt atcaagtgga taattaaaac tatatactag tttttcattg ttttgtgcat
     4681 tatgatccta cgactttttc agcatcaaac gaagttttaa attggcaaaa aacataaaaa
     4741 aacagactat ccctatcaga tagtctgcta aacgttgagt tatcagcgtt tttattattc
     4801 cgcagcgttt gcgtctttga gaccgtattt tttgttgaaa cggtccacac gtccgtctgc
     4861 ttgtgtgaat ttttgacgtc cagtgtagaa tggatgtgag tcagaagtta cttcgacacg
     4921 gatgactggg tatgtattac cgtcttccca ttcaactgtt tcttgtgaag tttttgttga
     4981 acctgacaag aatttgaaac cagttgttga atccatgaat actactgggt ggtaatctgg
     5041 atggatgttt tctttcatga ttttttcgct cctttgccct gctccatttg ctgaaccaga
     5101 gttgatttgt cgatttacaa cattaacatc ttaacatgtt tcagtcaagg ttgcaattat
     5161 cttttcagat ttctggtcgg atttttttta ccaaaggaga catcttgaaa ttctttgaaa
     5221 aattgctgat tgttttttgt tttacgcaaa aagcggatca gttgttccgt atattctaat
     5281 gaatctccag tcatattttt tcgaagtttc caaatttctt ctaattcttc acttggcatc
     5341 aataattctt cttttcgggt acctgatttt ttgatatcga tggctgggaa gatccgacgt
     5401 tcagataatt cacgtgaaag ttgtaattct agatttccag ttcctttgaa ttcttcatag
     5461 atcacgtcat ccatacggct tcctgtatcg actaaagcgg tcgctaaaat cgtcaaactg
     5521 ccaccttctt cgatgtttct tgctgccccg aaaaatttct taggtttata caatgcggct
     5581 gggtcaatcc cgccactcaa tgtacgtccg cttggtggaa cgactaagtt ataggcccga
     5641 gcaagtctag taatactatc catcaagata acgacatctc gtttatcttc gactaaacgc
     5701 attgcgcgtt ccaatactaa ttcagaaact cgagtatggt tttgcggctg cagatcaaat
     5761 gtagaagata ccacgtcgcc tttcacgctt cgttctagat ccgttacttc ttccggtcgt
     5821 tcatcaatca gtagaacgat taattctact tccggatagt tttcggtgat accatttgcg
     5881 atttctttta atacagaagt tttccctgct tttggcggtg cgacgatcaa tccacgctga
     5941 ccaaaaccga ttggagaaaa gatatcgatc attcgggtag atagtctttg cggtgttgtt
     6001 tccagttgaa gctgtctttg cggataaaga ggtgttaagg ctggaaaatg cggacgttct
     6061 tttgcttctt ccggatcttt tccattaacg ctttccacgt gcatcaaacc ataataacgt
     6121 tccgattctt taggtggacg ggcttttcct gccactttgt ccccatttct caaactgaat
     6181 cgtctgattt gtgaggcaga aatatagata tcttctgcac ttggtccata attgattggc
     6241 cgtaaaaaac cgtaaccatc ttgaccaacg atatccaata ttccttccat ataataaaat
     6301 ccttgtttct ctgcttgtgc acgaatcaca gctagagata attccttttt attcatctta
     6361 ctgtaataag gaattttaaa ttctttggca taactataaa cttcttttaa cgttttgttc
     6421 tctagttctg ccattgtcaa ataatcgctc attcagctcc cacttcctct atcatttcaa
     6481 ttgatgcccc taaggctgtt agtttctcga taatatgatc ataaccgcgt aagatataat
     6541 ctacattgta aatcgtcgtt gtcccttctg ccaatagtcc agcaatcact aaacaagcac
     6601 ctgcccgaag atcagaagcc acgacttctg tgccgtgaag tttatttgga ccattcacga
     6661 tgatcatatt tccttccacg gtaatatcag cgcccattcg tgctagttcc ggcacatggt
     6721 tcacacgttt cgcatagatc gtatcgatga tttctgaagt gcctgtagtc atcaatagta
     6781 acgcagtaag tggctgctgc agatctgtgg caaatcctgg ataaggatag gtcatgatgt
     6841 ttgcgggttt taattctttc gaggggtaaa cttcgatttc atcttcgctg attttcatat
     6901 ggacaccaat ttcctgcaat tttgctataa agctttccaa atgttcaaag atcacatttt
     6961 tcactttgat accatttcct actgcagctg ctaatgctag ataggttcct gcttcgatgc
     7021 ggtcaggaat catgaaatgc cgacagccat gtaatgtttc gactccttcg atacggatca
     7081 catctgtacc cgctcctcga acttttgccc ccatattatt cagtaaagtt gccacatcta
     7141 tgatttccgg ttctcgtgca gcgttttcaa ttactgtctg tccttttgct tttacagcag
     7201 ctaacatcac attgatcgtt gctccgactg aaaccacgtc catgaaaatc cgattgccac
     7261 gcaatccttt attttctgtc cgtaaataca tggcaccatg ttcatttgtc acttcagcac
     7321 ctagagcttc aaatcctttg acgtgtaggt caatcggtct agggcctaaa taacagccac
     7381 ctggcaggcc tactaccgct tcgccaaatt taccaagcag tgttcccata aaataataag
     7441 aagcacgcaa actattgatt tttccgtgcg gcatcggaat cgatacgact tctcgaggat
     7501 cgatttctag tatgttgttg gaaaaatgga cagttgcgcc catgatctct aaaatttcaa
     7561 ttaatgaatg aacatcttgt atgtccggca caccttccaa gatcactggg gaatcagcta
     7621 agattgcagc tggaatcaat gcgactgcac tattttttgc accgctgatc gttacttcac
     7681 cagctaaagg tcggtttcct ttgattacga tttttttcat ctgtttccac cttgtttatt
     7741 aatctttttt catggatatg cttgtatttt atcatagata aaaaaaagaa acgagcatgg
     7801 tctgccgttt taagtaaaaa agaatggaga gcgacttcaa aagtcgttct ccattcctat
     7861 atatcgtatc tgttatgtgg atgcttacgc tttgttagct gaaccgaacc agtcgatatg
     7921 agatttagca tcttcaacga ttgctgcttt acctggagct aataatttac gagggtcgaa
     7981 tcctttacct tctttatctt taccagcttc gatatattta cgagtagctg cagcaaatga
     8041 taattggaat tcagtgttaa catttacttt tgagataccc attgagatag ctttttcgat
     8101 ttgatcttga ggaatacctg atccgccatg taatactaaa ggcatgtctg gacctacagc
     8161 atcagcaatt gcttgtaagt ggtcaaaagc taatcctttc cagttttctg gatattgacc
     8221 atggatgtta ccgataccgc aagctaagta gtcgatacct gtagcaacca tttgtttgca
     8281 ttcttcaata tcagctaatt cgccgttacc gatgattccg tcttcttctc cgccgattga
     8341 accaacttca cattcaactg agataccttt tgcatgtgct ttttcaacaa cgtctttagc
     8401 taatttcaag ttttcttcaa atggtagatg tgaaccatcg aacattactg aagtatagcc
     8461 taattcgata cattccaaag cagcttcgta atcaccatgg tctaagtgga tagcaactgg
     8521 aacagtgatg ttcattgctt caactaaatc agtgattaaa tctttacaga ttttgtaacc
     8581 acccatgtat ttagctgcac ccattgatgt ttggataagt actggtgctt gttttgcttc
     8641 agccgcttca aggattgctt gagtccattc caagttgttt gtgttgaatc cgccgacagc
     8701 gtaaccgcct ttacgagcag cttttaaaaa ttcagctcct gatactactg gcataataaa
     8761 attcctccta agttttgtaa aactaaattt gttaagacaa ttcttaacca acgaccctat
     8821 tttaccaaac atttcggtag ttttctacta aaatcaatga aaatgaaaat tttcaagaag
     8881 ttttttgcaa aaaataaaat atccaatagc gctttaatcg aaaaaagtct tttgctttct
     8941 atcttttttc ctacaaataa taacaaatca gaaaaagaga aaagtgacaa gaaaattgct
     9001 tatccccttc tcttgctact tttctctttc tatcaagttc aaaaattatt ttgtttcgtg
     9061 atctgctaat gatgctccga caaatgcttt gattaatttt tgcgggcgat ttggacgaga
     9121 aatcaattct gggtggaatt ggcatcccac aaagaatttt ttctctggca gctctacgat
     9181 ttctactaaa cggttatctg gtgagacacc tgaaaagact agcccttgtt cttcaaacaa
     9241 ttgacgatat ttgttattga actcatagcg gtgacggtga cgttcttgca caacatcggc
     9301 tccatcatat gcttcagccg tcttcgttcc ttttttgatt ttacatgggt ataatccaag
     9361 gcgtaaagtt ccacccaaat tctcgatgtt ttcctgatca gccattaaat caataatatt
     9421 gtttgctgta tctggattcg tttcagctga tgacgcatct tctaatccaa cgacgttacg
     9481 tgcaaattcg acacaagcca tctgcatacc aagacaaata cctaaaaacg gaacgtcatt
     9541 ttcgcgagca taacgaatcg cttcgatttt cccttctact ccgcgatcac caaatccacc
     9601 aggaaccaag attccatctg cgtctttcaa acgttcttct acattttctc tcgttaattc
     9661 ttgtgctttg atccaatcga tctcgatgtc tgaattatag gcgaaaccag aatgtttcaa
     9721 tgcttcaact actgagatat atgcatctgg taactcgaca tatttaccga caagcgcaat
     9781 ccgtactttt ttcttcaagt tcaagacacg ttcttctaat tctttccatt ctgtcatgtc
     9841 tgcttctggt gtatctagtt ttaaatggtc gcaaacaatt tgatccattc cttgtgcttg
     9901 taaaactaat gggatggagt aaagtgtatc tacatctggc gactcgataa ctgcttctgg
     9961 ttcgacatca caaaattgtg ccaatttgtt tttcacgctt tgagaaacag gttgctctgt
    10021 acgtaccacc aaaatattcg gttggatacc taaaccacgt agctctttta cactgtgctg
    10081 cgttggttta gttttcattt ctccagcagc tttcaagtaa ggaatcaatg ttgtatggat
    10141 atacataaca ttatctgtac cgacatctgc tttcatttga cgaagtgctt caaggaatgg
    10201 caaagattct atatccccta ctgttccccc tacttctgta atgatcacat ctgaatcagt
    10261 cattttagct gctcgcatga ttttttcttt gatctcattt gtaatatgag gaataacttg
    10321 aacagttgct cccaagtatt cgcccttacg ttctttacgc aaaacttcag aatagatttt
    10381 tcctgtcgtc acgttggagt atttgttcaa attgatgtca ataaaacgtt cgtagtggcc
    10441 cagatccaaa tctgtttcag caccatcatc tgttacgaat acttctccgt gctgatatgg
    10501 gctcatcgtc cccggatcca cgttgatgta tggatcaaat ttttggatag ttacttttaa
    10561 gccgcggttt ttcaataagc gtcctaaaga agctgcaaca atcccttttc cgattgatga
    10621 aacgacaccg ccggtcacaa aaatatattt tgtcataagt tcaaaaactc ctttttaatt
    10681 tatgtgcagg aaaagtctca gacaataaac aaagctccct attcttcaat aagaataggg
    10741 agccgaaatt tctgtaaact tctgaccctc taaaagggtg cccaaggtgt ataatacaag
    10801 ctgactaagg gaaagtcaag tactcaaatg aatactatcc gcttacaatt ttttcttttt
    10861 cgatatcctt tgagaaatta tttcttaagt cctttatgag tgcaaaaaaa atttaaccca
    10921 cataataagc ttgatataaa catagctaac gttaaaccta agcaaatcag aaaatcgtta
    10981 ctgaaaaatt ttaagtgaaa taatagataa aaaaataaaa caaacattat gatagataat
    11041 atcccaccta acaaagaact actttttcct acttgcagca acagtttata tagcttatcg
    11101 tagaaacaat ctttttcttt cacccttatt tacctccttt acgtaaaaaa cgtagtccac
    11161 agttctcgga tttttggcat atcttttcta ttaaataata tcaaaaacaa aacaattcca
    11221 ctaatttttt caaagatgac ctttttttca acaaaagtgc cttgtaggga ttcattgaag
    11281 gtaacgaatc acataagtaa aaaataagga gatgcgacaa aagtcgtgcc tccttatttc
    11341 taaataaacg gtgttctcct gccaactcct cggtcacaag tcacaatatc catcaaacat
    11401 gagaagctgt ttttgaaatt atttcttgtg attcggagtt aaatggtagg ctcacaacct
    11461 ctggataaac ggtattcaaa agccagtttc tcggtattaa gccaaattta tgcaaaaatg
    11521 tagaaagcca ttttcacaaa atttttctta atactcgaaa ctaaacgtct ttttcaaaac
    11581 ctcttggtaa acggtgttca aaagcttgca tctgcggtaa taagttcagc caattcaaaa
    11641 acaagaaaag ctgtttttgc ttggctattc ttattacttg ttgcagggca gctttttcac
    11701 aacctctcca ctaaacggtg ttcaatcagc tgaccctcgg tattagctca aaaaacggca
    11761 aaatatgagg aactattttt ccgttagttt ctctaatact caggtcaaaa cgctgatttc
    11821 acagcctcta attcacggta ttcctgtttt aataaagttc tagatattgt tctctttccc
    11881 actcggaaac agtttgtctg aatgccgccc attcgagacg ttttgcttca acaaaatttg
    11941 agaagatatg ttgtcctaat gcgtcaatca ttacattatc cttacgcaat tctttgatag
    12001 cattatggat agttgaaggt aagtcctgga tatgtgcagc ttctctttct tcctcatcca
    12061 ttacataaat gttacggtca acagctgctg gcggtgtgat ctgattacgg ataccgtcta
    12121 gtcctgcttg caacaatact gccattgcca agtatgggtt tgcagatgga tcaactgacc
    12181 gcaattccaa acgtgtggat aaaccgcgag attctggtac acgtactaat ggcgaacgat
    12241 tacggccact ccatgcgaca taaacaggcg cttcataacc tggaacgaga cgtttataag
    12301 aattgactgt tggattgcaa acggctgtgt aggcacgagc atgtttcagc aaaccaccta
    12361 agaaatgata agctgtttca cttagttcca attcaccgtc tttatcatag aaaacatttc
    12421 cttcatcatt gaacaaagac atattgcaat gcataccaga accattgatc ccaaataacg
    12481 gtttcggcat gaaagtcgcg tgaagtccat gcttgcgtgc aatcgttttg acaacgagct
    12541 tgaaggtttg gatattatca caagcatcta ccacatctgc atatttaaag tcaatttcat
    12601 gctgtcctgg cgctacttca tgatgtgaag cttccacttc aaagcctaga ctttctaatt
    12661 ccaacacgat gtcacggcgg caattttcac ctaaatctgt tggtgcaaaa tcaaaataac
    12721 cacctttatc attcaaggtc gttgtgatcc caccgtcttc gtctaattta aataagaaga
    12781 attctggttc aggtcctaaa ttgaaagagg taaaaccaag gtcgcgcata tctttcaatg
    12841 ctcgtttcaa attcccgcga ggatcgccgg caaatggtgt cccatccgga ttatagatat
    12901 cacaaattaa acgagcaact tttccatgag cactttccca cgggaagatc atccaagtgg
    12961 agagatctgg atacaaatac atatcgcttt cttcaattcg aacaaatcct tcaatcgaag
    13021 aaccgtcaaa catcatttta ttttctaata ctttgtcgat ctggctcact ggaacttcta
    13081 catttttgat cgtacctaag atgtctgtga acatcaatcg taaaaatcgt acgttttctt
    13141 cctcaatgat tcgtttgatt tcttcacctg ttatctgttt ctttaccatg gtatccctct
    13201 tcctttttta atttattcag tcaaagaatt gtctttgcaa tccttgcgac tgataatgac
    13261 ctgttttgct taacccacct tgcgccaaaa tctcatcacg aaaaatttgt cgaacgtcat
    13321 gatctgtcaa cggcttcttg ttttttgctt cttgtttttt cgcctgtgcc atttcgtata
    13381 cacgtttgat cccagccata ttcaatccct ctgaaagata gtctttaatc tcaagcaaca
    13441 tatcaatatc attcagcgaa tacatacgtc ggtttccttc gcttcgttcc ggatgaacta
    13501 aatcttgttc ttcataatag cgaatttgcc gagcagacaa atcagtcagc ttcataactg
    13561 taccaattgg aaatacggac atagatcggc gcaattcctt ttctcccatc agacgtcccc
    13621 tttcgtttta taagttggct aaagtgtacc aattaaaaaa aatattgtca actgcatatg
    13681 ttaggttttc taacatgaga tctctatttt ttataaaata acaaaaaaat aagaacacaa
    13741 aaaaggtatc tagttgattt tcgtaccaga taccttttat tttttattat actgtactat
    13801 cctttattag tgtttttatt
//
LOCUS       DACGLW010000080        13745 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.083, whole genome shotgun sequence.
ACCESSION   DACGLW010000080 DACGLW010000000
VERSION     DACGLW010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13745
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.083"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            complement(45..1364)
                     /locus_tag="IXT53_002289"
     CDS             complement(45..1364)
                     /locus_tag="IXT53_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ4906083.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIISL
                     IYAKSIKNNIQEIKIVTDKMMKLDKKMSLKVSSNDEVGELKQQINDLYSTLLRTIDDL
                     EFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYINECIE
                     IVDSLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKITVNNYILD
                     ENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIF
                     DVKFDLNKENSNGLGLYIVSNILNNYNIEYKVLQDEEFFIFKIKIFS"
     gene            complement(1361..2014)
                     /locus_tag="IXT53_002290"
     CDS             complement(1361..2014)
                     /locus_tag="IXT53_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ4906084.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            2302..2822
                     /locus_tag="IXT53_002291"
                     /pseudo
     CDS             2302..2822
                     /locus_tag="IXT53_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2724..3440)
                     /locus_tag="IXT53_002292"
     CDS             complement(2724..3440)
                     /locus_tag="IXT53_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HAQ4906085.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(3564..3749)
                     /locus_tag="IXT53_002293"
     CDS             complement(3564..3749)
                     /locus_tag="IXT53_002293"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906086.1"
                     /translation="MTKALIFDFDGTMIDSEWMFYESIHHYLLKPIYWSSSAIKFLLQ
                     TINKVLGRQQNCPFMKG"
     gene            3976..5249
                     /locus_tag="IXT53_002294"
                     /pseudo
     CDS             3976..5249
                     /locus_tag="IXT53_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250512.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(5356..6387)
                     /locus_tag="IXT53_002295"
     CDS             complement(5356..6387)
                     /locus_tag="IXT53_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4906087.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(6394..7224)
                     /locus_tag="IXT53_002296"
     CDS             complement(6394..7224)
                     /locus_tag="IXT53_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ4906088.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(7221..8027)
                     /locus_tag="IXT53_002297"
     CDS             complement(7221..8027)
                     /locus_tag="IXT53_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ4906089.1"
                     /translation="MKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLANYS
                     DIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLAANF
                     AGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFPIPLGVLFLYPSMK
                     EVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALTSSN
                     VNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(8033..8857)
                     /locus_tag="IXT53_002298"
     CDS             complement(8033..8857)
                     /locus_tag="IXT53_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphatase"
                     /protein_id="HAQ4906090.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(8847..9947)
                     /locus_tag="IXT53_002299"
     CDS             complement(8847..9947)
                     /locus_tag="IXT53_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HAQ4906091.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            10125..10910
                     /locus_tag="IXT53_002300"
     CDS             10125..10910
                     /locus_tag="IXT53_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HAQ4906092.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            10943..11326
                     /locus_tag="IXT53_002301"
     CDS             10943..11326
                     /locus_tag="IXT53_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HAQ4906093.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            11402..11533
                     /locus_tag="IXT53_002302"
     CDS             11402..11533
                     /locus_tag="IXT53_002302"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ4906094.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            11502..11738
                     /locus_tag="IXT53_002303"
     CDS             11502..11738
                     /locus_tag="IXT53_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ4906095.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(11816..12787)
                     /locus_tag="IXT53_002304"
     CDS             complement(11816..12787)
                     /locus_tag="IXT53_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HAQ4906096.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
     gene            13421..>13745
                     /locus_tag="IXT53_002305"
     CDS             13421..>13745
                     /locus_tag="IXT53_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ4906097.1"
                     /translation="MQGVIAMMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFE
                     FIYPIVEATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGYS
                     FEEKIP"
BASE COUNT         4169 a         2991 c         2384 g         4201 t
ORIGIN      
        1 ataagtctct atttttcaat aactattgca aaattatatc ttaattaaga aaatattttt
       61 attttaaaga taaaaaattc ttcatcctgc aatactttat attctatatt gtaattattc
      121 agaatattac ttacaatata taaacctaat ccattactat tttccttatt taaatcaaat
      181 ttaacatcaa atattttatc catattcgaa attttattgt taccgtatga attctctata
      241 tataaccaat cattaactat cccaatatta attaccccat ttacatcagt atattttacc
      301 gcattactaa tcaaattaga tagaataatc tttaaagctg tttttcctat ataaacattc
      361 tcgtctaata tataattatt gacagttatt ttcttttgat ttgctaatat ttcatacttt
      421 tttaatatgt cttctaatgt atcatttatt ttcaaatatt cttcatcatt attcaaattt
      481 tctatagaat gaactgataa tatttgagaa atattttttg ttaaactatc aacgatttca
      541 atacattcat taatataaat atctctttct ttatattttc caatattgta tttcatgttt
      601 tccagtatta ttttaagact agcaagaggg gttttaagtt catgggatgc acctttaaaa
      661 aagtcatatt ttaacttttc taattttaaa atttctttat ttttaaattc caaatcgtca
      721 attgttctta ataaagtaga atacaaatca ttaatttgtt gttttaattc tccaacttca
      781 tcattagagc taacttttaa actcattttt ttatcaagtt tcatcatttt atcagtaaca
      841 atttttattt cttgtatatt attttttatt gattttgcat aaattaaaga aataatagca
      901 gaaaataaaa ttgatattaa taaagaatat ggtaaaaatt taaggcttaa atctttcgca
      961 tctttttgca tatcagctgt agaaacaaat tgtagatttg tttttttacc atcattaagc
     1021 tttatttctc tttcttcaat tatcaaagaa ttactatcac tttctaaatt aacatttata
     1081 ttatctttga tttgtatttc attcttatta tttttttctt ttatgaatac ttttatttca
     1141 ctactcttgg aataaaactc caaagtttgt tctatatatt ttatttcttt tccattcata
     1201 ttactagaaa tttcatttgc catattatga atcttttctt ttctcgtctc caaatatgtt
     1261 ttaggaaaga taaaaaaaat taatgaatga actaatataa ttattattcc aagaacagaa
     1321 aatatttgta tgaacatttt tggaaatatc tttaattttt tcattttctc tccaatttat
     1381 atcctacatt tcttatagta gttatacagt ctaattgtaa tttttttcta agttctttta
     1441 tatatacatc tattactcga tcataaggag tttcttcgct atctttccat acataatcaa
     1501 taatttgcat tcttgttaac acttgtccat tattatccaa taaacatttt aatacttcta
     1561 gttcttttgc atttacatct atttcttcac catttatttt agctgtatag ctattaaaat
     1621 taaccgatag atccttatat tcaaacttct ctaaatgtcc ataattcttc ttaattaaag
     1681 aatctattct agcttttaag accggcaatg aaaatggctt ttctacatat ccgtctacta
     1741 aattagtaaa tgcatcaatt ttatattctt catcactaaa tgcagtcaag attagaattg
     1801 gtaaattgct tttctttcta atttctttca acacttctaa accatttata aaaggtatct
     1861 gaatgtctaa gataaccaaa tttatatcgc tattaaattt tgacaaggct tctcttccat
     1921 ctttagcttg aataacagta tatccaaatt ctgaaagata ttcacttatt ccctctctta
     1981 tcatattatc atcttcaaca gttaatattt tcatatatac ctccacttaa gcctatacac
     2041 tagatattat acagcatatt atggctctat aatatctagt gttggtgacg ccttctgttg
     2101 ccaatacttt ttcttttttt taataaatgc aaacagacac cttgaaatcc tttagaaaaa
     2161 aggcttctat aatattttgt gttgttggaa aatcgaaggc gattttctac taacactttt
     2221 tcttcgtttc aactcaaaaa gagactcact tttggtaaaa tttagtcgac caaaacatca
     2281 ccctaccaaa ggagtcgctc tatggataat catactagaa aattactcgg attaacagat
     2341 aaacatttat tttttgatga agaatggctg attgaaaaag aatataaagg cgttcaagcg
     2401 cagtttatta aagggagatt ggatgactca ccttcccatt gtgaacactg tggatcgatt
     2461 cgtattatcc gcaacggttc ctatacgaca cgtactcaga tgttgaaagt caaagaaaag
     2521 ttgactattt tagaattaaa acgtacccgt tttctatgct atgattgtgg gcagaccttt
     2581 tctgcgaaaa cggacttagt tgatgaacat catcaactaa caaaggaact gaagcaggcg
     2641 attctcatag aactgtacga aaatcaatca cgtaatctga aaatcagaag aagtaccgtc
     2701 ggctcaagcg atattggaaa ctcttactca aagattccac gacattggag ccgctcaaac
     2761 ggcattatca tcgactgttt aaacgaccaa ttagtcaaac agaaatcgtc gatgaaccgt
     2821 tgtgcacaat ggtgtcctga acgtacagcg acaccttcca tatccagagc agtcgccaca
     2881 tcacggggat ggatgccatc caaattaaaa gcaaccacgc cagtatgatg ttcaggatct
     2941 tgcggtccgt aaatggtgat accgccgatc gcttgtaatt taggtaaaac gtaggcaacc
     3001 agttcttgct cataacgatg aatattctcc ataccaatct cattcaagta atcgattgct
     3061 cctgcaatgt tcggagtacc agcttcaaat ttccatggca gctctttcca tgaactatcg
     3121 taaagattga caaaatctat catttcaccg ccgaattcga ctggctccat ttgttctaac
     3181 aattgacgtt tgccatataa tacaccgatt cctgtcggcc cgcacatttt atgcccgcta
     3241 aaggcgaaaa aatctgcatc caacgcttgt acatcaactg tcatatgcgg tgctgcttgg
     3301 gcaccgactg gactatcctc aactacaaca gcctgttcaa ccctcagccg tttcaagcac
     3361 aacaaataac cttctggatc tggtttgccg acctgaagat catcgatcgt cacaaaggaa
     3421 tcaatttttt ccttaatcat cggatgtgct tcaaaaaaat agtctagctc ccgcagttca
     3481 gaattgagac tactccgatt ttccagtgct gctgatccgc tgctcaaaat gttcagcatc
     3541 cactggtctc ccaaccgctt gacttatccc ttcataaaag gacagttctg ctgccgtccc
     3601 aacaccttgt tgatagtctg caaaaggaat tttatagccg aactgctcca gtaaataggc
     3661 ttcagtaaat agtgatgaat actctcgtaa aacatccatt cactgtcaat catcgtccca
     3721 tcaaaatcaa aaataagtgc tttggtcatc ttctttcccc cttacccaaa acaagcaaaa
     3781 gctgtgtcct gctctctcta cagtatcagc ataaaaactg ccgtctgaaa agtcaaaggt
     3841 gaaagaaatg tggcgctata atttctagga ctgatagtaa atcattctat cagtcctttt
     3901 gagcataaaa aaacaaaaca tacagaagct ccgttagaat tgaatcacca caacacaatc
     3961 aaaggagcgt tctgtatgcc tgatcttcat cttatcaaac tattactagg aatcaaagat
     4021 ggaaacattc aaatcactca tgtcgagaag aaaaaaatac attcaatcaa cagtatcgtt
     4081 gtttctgcca cgctcattaa aacgattcag ccctgtcctc agtgtaaaca ggtgaatcaa
     4141 catgggctga tcgttaagaa cggaaagaaa aaatcgctgg tccttctcaa taagtgtgcg
     4201 aatcaacgga cttatcttgc tttggcaaag caacggtacc tctgtcgtgg ctgtcacacc
     4261 tattttacgg cgaataccta cattgttgaa aaaaactgtt ttatcgccaa acaagtacat
     4321 tataaaatat tggaggaatt gactgaaaaa caagcaatga cgacgatttc taaacattgc
     4381 ggcgtttctt ggtcaaccgt ttcgcgaaca ctcgcttctc tgctccccat gacaaacgtg
     4441 aaaataaatt ggttgcctcg ctgttcattg gtagatgaat ttcgttcatt gaaaaatcaa
     4501 gtcggtccct attctttttc ttgtatggat ggcgatactg ggaaattact ggatattctt
     4561 ccttcacgta agaagaaaga tctcatttct tattttatgc aatttgaacg acgtgcacgt
     4621 ttaaacgtaa aaatactcgt aacagatatg aacgcctctt atgctttgct gataaaagag
     4681 tgctttcctc atgccaaact cgttgtggat cgctttcaca tcgtcaagca cctcattcgc
     4741 agttttaagg atatttgact atgagtcatg aaaagcttcg accgaaacga tccaatacaa
     4801 gccaaacact atcgacaagt gaaggcttta tctcgacttc tgatcaaacg acaggacacg
     4861 cttgtttatg ataaatggac aaaatggcgt aattttggtt gggcctattt aaccgaaagt
     4921 gaagtcgtag aacgtctact ttctacttct gatgaactac gaatagctta tgcttactat
     4981 caagaaattc ttcaagcttt ttatgacaaa gaagcggaca tcttctttca gcttgtaaaa
     5041 acaatgccca agtcggttcc aagagaactg catcatataa aaaaagcgtt tataaactat
     5101 gaatccggca ttcgtttagc cttagagttg ccttactcaa atggcaaaat agaaaatctg
     5161 catacccaca tcaaagcatt gaaacgaata gcttacgggt taggatgaga accagaattt
     5221 tcttaatcaa tcatctgatc acttattaaa cataaaaaaa catctaaagc agaaaatcaa
     5281 ttctacctta gatgttctta tatcactcta tcagtcctat ttgacaaaga gccagaaatg
     5341 tttgccgact atacactagc tttgttgtgc aaaatgctga gtatcaattt ccacagggat
     5401 tgctagaata tctttcagct gaatgtataa ggcatcgcta attttctctt cccgatcgtt
     5461 caaccgatcc actaaaattt ctttgccctc cagcgtttga aaaacatacc gttgaatgtt
     5521 gcctaaaata aatgattcct tccatagaac cggaatctta accccctgag gaaccggttc
     5581 aaattgaatc agctccggtc ttaaataata aatgcagcga ttgcccttag cggaaaactc
     5641 gtgggtatac tgattcagct cataccattc gaaacgatta tagttgccga taaattccgc
     5701 aacaaatcgg ctctgaggat tctgatacaa ttccttcgag gtggaaacct gagccacccg
     5761 cccagcttcc atcacaaaaa tccgatcact gattcgcatc gcctcttctt ggtcatgagt
     5821 cacaaaaatc atcgtcatcc ctagcgattg ctggatagcg cgcaaatccc gctgcagctg
     5881 ttttctaatt cgggcatcca aagcacttaa tggctcatcc agcagcagga ctttcggttc
     5941 agtgaccaat gagcgagcca gtgccacccg ttgtcgttga ccgccagaaa gaaactggac
     6001 atttttatct gcctgctccg tcatatccac caatgatagc atctcaaaaa ccttttcctg
     6061 aatcacctca ggactcagct tctttacctt caaaccaaag gctacatttt caaaaacagt
     6121 catagttgga aacaatgcat actgttgaaa aacaaagccg atattgcgat ccttcgtagg
     6181 cttatcggca atattctcat tgtccagcac aatctcaccg ctctttggtt gttccaatcc
     6241 cgtgatacag cgcagcaacg tagactttcc gcacccgctg ggacctagta aagtaaccag
     6301 ctcacccttt tcaatctgaa tttcatcaat agctaaaatc tgtttttcat caaaaaacag
     6361 attgagatca tgtatttcta aaaacatttt ccgtcacaac tcctttaagg gcttcttttt
     6421 cgactgcttc tcttccctcg tctgcttaag ggacagctgt tgcttttgtc gataagtcag
     6481 cataaaggta gttaaagcaa ttcccaccat aaacagcaaa tacaaaacca tcacagcgct
     6541 agccaaatgg ccgttttcgt tcatccgccg cagcatatag atgcgtaccg tctgaaatcg
     6601 accgccaatc aacaggttcg tcagcatgta ctccccaaaa accgaagaaa aaatcatcag
     6661 agaaactagg gtcacaccga tacggatgtt tggaaacagc acctggataa taatcgtaga
     6721 catcggggcg cccaaggttt ctgctgcatc cactaattcc ttcagattaa ttaagcgcat
     6781 ctggttgtta agactaagat agacaatcgg caacgccgta ataaacaatg cacctaccaa
     6841 cactacaaac attgggatac cagtttcagc atacgttcga agcaaagccg ttaccaaaat
     6901 cactcctggt aaagcatacg gcagcaggag cagcttttcc agccaacgat tcaaacgagg
     6961 gaaatacaaa tgaatccaaa taatggtggg aatcatcaac aacagaatcg tcaccaagac
     7021 cacacctgct aataaaagtg agcgacccac cgcagcaata aattccctgt ccgtcagcag
     7081 ctgttggaac cattgcaagg taaaatcact aggtaaaagc gattttgacc atttagtgga
     7141 gctggcatac aaaatcgttc caataatcgg cagcaagtaa tacaggacaa agcagaacaa
     7201 aatcggtttt attaactttt tcacgtctgc accaccttgc ctttcttttg aataatcaac
     7261 attgccgtta ccagcaggac acctaaagcg actgccaacg cactgcctaa attaggcttg
     7321 gcgaacacat ctcccgacac taaagccgca attcgaatcg ttaaaagatt tacattactg
     7381 ctggtcaggg catacgctgt ttcataggtc cccatgccat tagcaaataa gatgacaaag
     7441 gtgctggcaa tcgatggtcg caaaaaaggc aaagccacct tgaaccaagc atacgctgat
     7501 gaagcgccca gcaattgtgc ggactccagc cattcctttt tcacttcctt catgctggga
     7561 tacaaaaaca agacccctaa cggaattggg aaataggtat acgtgatggt caatccccac
     7621 caagaataca aattgaagtc cgctagaaat ggcaaaatca ttttcagcac acctacattc
     7681 cccaacaaaa taataaaaga gaatgccagc ggaatcccag caaaattagc tgccaaattg
     7741 gaaacaaagg tgattttctc ctgcaattta gtcgatagat tcattaagca taaacataaa
     7801 ataaacgtcc cgattaaccc aatcacactg gagctgacag caatcagtac gctgttaaca
     7861 aaggcctgtg tgtaatattt gttgctgaaa atatcactgt aattggccaa tgtccactgg
     7921 ttagttaaat cactgtgcaa gctgcacaga accataaaaa gcagcggcag cagtaaaaat
     7981 gcacagaaga taaaggaggc aggaaccaga accaagcgtt ttttcatctc tttcatcccc
     8041 tcatcaattc ttgaatgtcc tgcattttct ctgacggtgc aattcctaat aaccagcaaa
     8101 acaatggagc cgccagcagc tgtggtacag tttgttcctt cagcttttgg cgtttatttg
     8161 aaatcaaata aaaaggaact tcacgatgtg cagccaagct gccgccatgc agtccgtgcg
     8221 catccatccc gtgatccgca gtcaccacga cttgataacc tttttgcagc cattcaggaa
     8281 ggtaaatagc caataatctg tccgcctgat taaccgctgc tgcatattca tgagaatcgg
     8341 cggtaaattt gtgaccgaca tcatcaatat tcatcgaatg tacgaccaaa aagtctggct
     8401 gatactgatt cactaaataa tgggcatccg caaataaatg cgaatcaggg taagtgtcct
     8461 cactataaaa gatgccatgc tgaatcagtc gctcctcctg atgctggatg cgatcaacca
     8521 aacgattata gggggcttga ttgtacaact cgctgtacca gtggtaagcg gcggctcctg
     8581 tttttccgcc agcttcctta actaattcaa acagcgacca ctctttggat cgctgagcat
     8641 ggtaattact atagatgcca ttttcatagg ctggaacgcc ggtcatcagt acttcgtata
     8701 acggacgtga attgctgggc agctcacttt ttaccttcaa taagcttgct tgctggtgct
     8761 caactaaatg attcatgaac ccaagctggg cgacggctgt gtcgtagcga cagccgtcta
     8821 gtataactac aatcagtttg tcaagtctac tttgcatagg tcagtacctc cgcctgccaa
     8881 agctctggaa gctctacaga ggtcttgttc caagcttcat tgtcttcaac tggcaccgcc
     8941 gcttcataag catcctccgg cagtaatttt tcagctactt ctttaggcag ctctaccttg
     9001 cgaataggtc gcgcatagcc ctttgccaaa ttgatttgcc cttcatcaga taaaatatat
     9061 tcccgtgcta gctttgccgc attgggatga ggggcatttt tattgattaa ggttgtatac
     9121 ccgctaatga tggagccgtc tgttggcaca gttacctcaa aacgctcgga gtcaatttgg
     9181 tcacgataat ttagtccatt gaagtcccaa acaactgcca catcgatttc acctttttca
     9241 agatttgcta cgctggcatc aacggtggac aaacggcctt tttctgccat ttttttgtag
     9301 taattcagac ctggctgaat atccttttcg tcacctccgt tggcaatcgc cgcagccaat
     9361 acagcaaatt gtgcttggtt ggcaatcgtc acatccccca cgctaacttg atagtcaccc
     9421 tcttgcagct ccttccagct agtcggtgct tgcttcacat tctctttatc ggtgataaag
     9481 gaaatggttc ccgtatagct taacaaccaa tccccatcat catcctttgc ccaatccgga
     9541 atttcctccc aatagttggt cttataaggt aaggttaact ctttgctttc agccaacgga
     9601 ccgaagctga taccgacatc accaatatcg gcggttccat tttttccttc tgattcaaac
     9661 ttagccaatt cttctgcact ggacatatcc gtatccacgt gacttaagcc gtattctttt
     9721 tcaaggtcgt tccaagtgcc tacccaattt gcccaagtat ccggcattcc caagctggca
     9781 atctctcctt cttccttggc tttttctata atcgtatcta aagagtcgtc tgccgcagcc
     9841 gattgcttcg ttgcttcacc tgagccgcag cctgtcagca ggatacttac tgttgctgca
     9901 caaaataaac cgatagtcat gttacgaatt ttttcattca ttttcattca ttcttctccc
     9961 ctttcgataa ttccattcta acaaagcttt gtaatgagag aatcagtttc ttgaaaaggt
    10021 ttcatgaaca atgtaaaggt tgtatatggt ttgtaaattt cgacgaattc ccgttaacct
    10081 tctaaagctt ttgcggtatg atagtagaaa aaaggaggct ccctatgcag tattatgatc
    10141 agcatctcca cacgtatttt tcctttgatt cagaggaaaa atttgaaaac tatttagcct
    10201 atcagccgga gtttttcgtt tctacggatc actttgattt aaacaatcca tctaccggaa
    10261 aagatgacat tccggattat ccagcctata taaacaaact ggactcttta aaagaaacct
    10321 attccaccaa atttctgcga ggtatcgaga ttggcgttgt tccaggacaa gaagagcgga
    10381 tcgtggatta cctcgctcag catccttatg atttaaagct ggtaagtatt catcaaaatg
    10441 gccgctttga ttatatggac gatgtggttt taaccaaaga tcccattcaa actaccaagg
    10501 aatattttga tcagatgttg caggtcttga cggattttcc agaaggagat attttgactc
    10561 actttgatta tggattacgc cgcttcacct tcacagcgga agaattcgcg gagcattttg
    10621 aggctcagct gctaacgatt ttcaaaaagg ttgttgaacg agaattggcg atggagttaa
    10681 atgccaagag cttcttgaaa tacggcaacg aagaccttta ccgttatgcc gttcctttgt
    10741 atcaaagtgt cggcggaaag ctctttacct taggctcgga tgctcatgtt gccgaagatt
    10801 atcagctggg ctttaaaaaa atgagtcagc tgttggcaga ctgtcaggta gaaaagttgc
    10861 tagtcattca aggaaaagaa cgccgcctga cgccgctgcc gattttgtga ttcctatata
    10921 atagaagaaa gtggtgaaaa aaatgagcaa cctgtgggac tacaatcaag aggcacccat
    10981 ccattattta attgcgcgac attgggatgc gttgaaaatt gaggcggttt gccgatcctt
    11041 gttagcagcc gttccaaaac aacaactgga aaattttttg gtggctgaca gcttgcagcg
    11101 ggaaaaagtt caggcttatt ttgcagcctt taaagatcag cctttggaat atttgcatgc
    11161 gcagtttcat cttttttatc aagtagccgc cccagatgac tacaacgatt tacgaggaca
    11221 gctgcaattg acctttcaag cggatgaaac cgcctatacc gttttattgg gaatggctcg
    11281 ccttggtgat caggcgaagg tcgagtggcg gattttcgat atttgatggc aggatcaagg
    11341 cgtttcagtt gcaaaccttc ctctctttca attagaaaaa ccgcagcagg aagaagtttg
    11401 tgtgctggtc accgatgctg gtgaaattcg tctccgctta tttcctgacc aagctcccct
    11461 ggctgtagcc aactggcgct ccctagctga gcaaggcgtt tatgacaaca cacctttcgg
    11521 ctcgtgtcat taaggaattt gttattcaag gtggcgcttt ggatggttct ggtgatgaag
    11581 ctgtttccag ctatgagggc tttttcgctg atgaagtgca tcgcggactt tatcatttta
    11641 atggcgcctt ggccttaggg gaccacggcc cccacaccaa cggcaaccag ttttttattg
    11701 tgcaaaacac aaaggcgcag gctgacctgc taatgtaaag caagcatact tggagcttgg
    11761 aggactaccg gagctggatg gtcgttatac tgtttttgga caggtatacc aagggttaga
    11821 aatcgtagca gcgatcgcag ctcagccgac aaacagtgaa gatgtaccga ttgagccagt
    11881 gttcattcga caaattaact tcgaaaaaac atcgtaaaaa gacactcact gacttcagca
    11941 aaagctctat tcttgagctg gctgcagcta gtgagtgtct ttcatttatc cataagataa
    12001 agcttggatt caataaataa ataaatgttt tggttaaagc tgtgaccagc aaatgaggaa
    12061 ccgtgcgagc cagttgatct gccaatgcaa acatttcttt aggtgaaggc gctggcgtgt
    12121 ctgccaacgg atactccagt cccaatgctt ggtaaccgtg cataccatag cagtggtacg
    12181 gtagtaattc taacggtacg aaccgatcaa gatctgccac aaaacaagca atcgtcagca
    12241 gctcggtcag gttcatattg atacctggaa ccacaggaac tcgaattcgc acttcgcagt
    12301 taggatactc aattaaaaag cgacgaatat tcttcaaaat ttgttggtta tctaagccgg
    12361 tccattgttt gtggagcttg ctggtaaaca tttttacatc cacaaacaat gtatccacat
    12421 agggagctac cttttggatt tcattataag caccacaaaa gctggtttcc agtacggtat
    12481 tgattccaat gtatttcgat ttttgcaaaa tttctttagc aaaatccgcc tgaaccagtg
    12541 cctcgcctcc gctaatggtc actccgccat ccgagtgaaa gtaaaaaaca gcatccttct
    12601 caatctcatc cattacctct tcagcggtca tggtctggcc aaatcctttt tcaatctgct
    12661 tgctttgtga ttcgggaatc gaacaccagg cacagcgtaa tgggcagccc tttaaataaa
    12721 caacggtccg taaaccagca ccatcatgta gagaaccctg ttctattctt aatactgttc
    12781 cttccatata gatttttgaa ttacacacta gtttaaacta aaacacacaa ggaaaaactt
    12841 accatttaat cataaaatta gctcatataa atatactagg atgtaaaata gactggtcaa
    12901 aatctggtca aaaaagagga ctaatacagg tagtcctctt ttttgttatt ttaatattct
    12961 aaatcatttt ttattggttt attcccttat tcgatgtaga ctcaaatcac tatatcacta
    13021 gatatcaaac ggcgtcatac ggaatcaaaa acatcaaatt tgacctgtta aatggtcatt
    13081 gaataacatt caaaatcaat aaaacggagt cagacacgga gtcaaaaacg ctgagagtaa
    13141 aaaatatata cagccttgta ggtaaagtac cacagggctg tatatttttt atttcagatt
    13201 accttaaatg attccattta ctttttatct cattttttcc tactagttag tatcaaatac
    13261 tctcctattt tctcaataaa ctatttttgg atacatggta tctgaaacag ctaaaacctt
    13321 gtcattacag caaaaaatca cgttatccag actgtagaca aagtccattt tatggatgat
    13381 gtctacagtc ttttctttta taatagattt atgaaaacga atgcaaggag tgatagctat
    13441 gatgtctaaa aacaatacga atggtagaaa tcaatttgcg atgcttacga ttgatgatct
    13501 agttcctcaa gatcatctcg ttcgaaagat tgatgccgcg cttgattttg aatttattta
    13561 tccaatcgtc gaagccactt actccgattt aggtcgccca agtatcgatc cagtgattct
    13621 gatcaaattg gtgtttattc agtatttatt cggtatccgt tccatgcgac aaaccattaa
    13681 ggaagtcgat accaatgtcg cttaccgctg gtttttaggc tactccttcg aggagaaaat
    13741 ccccc
//
LOCUS       DACGLW010000081        13700 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.084, whole genome shotgun sequence.
ACCESSION   DACGLW010000081 DACGLW010000000
VERSION     DACGLW010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13700
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.084"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            complement(<1..901)
                     /locus_tag="IXT53_002306"
     CDS             complement(<1..901)
                     /locus_tag="IXT53_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HAQ4906098.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFSE"
     regulatory      complement(925..969)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            1184..2152
                     /locus_tag="IXT53_002307"
     CDS             1184..2152
                     /locus_tag="IXT53_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ4906099.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMD"
     gene            2265..2543
                     /locus_tag="IXT53_002308"
     CDS             2265..2543
                     /locus_tag="IXT53_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HAQ4906100.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            2661..2897
                     /gene="secG"
                     /locus_tag="IXT53_002309"
     CDS             2661..2897
                     /gene="secG"
                     /locus_tag="IXT53_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HAQ4906101.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            3009..3755
                     /locus_tag="IXT53_002310"
     CDS             3009..3755
                     /locus_tag="IXT53_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HAQ4906102.1"
                     /translation="MRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKADYTV
                     YAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFAVRA
                     LEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSLAEQ
                     QLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQWYPE
                     SGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            3758..6115
                     /gene="rnr"
                     /locus_tag="IXT53_002311"
     CDS             3758..6115
                     /gene="rnr"
                     /locus_tag="IXT53_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HAQ4906103.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKARNGKFGKRE
                     GKQAGKRSEKNGKKNKRYSDSKQTFGKKKGKKPFYKDVKKKKKKK"
     gene            6137..6601
                     /gene="smpB"
                     /locus_tag="IXT53_002312"
     CDS             6137..6601
                     /gene="smpB"
                     /locus_tag="IXT53_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HAQ4906104.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIVRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            6903..7622
                     /gene="atpB"
                     /locus_tag="IXT53_002313"
     CDS             6903..7622
                     /gene="atpB"
                     /locus_tag="IXT53_002313"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HAQ4906105.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            7675..7890
                     /gene="atpE"
                     /locus_tag="IXT53_002314"
     CDS             7675..7890
                     /gene="atpE"
                     /locus_tag="IXT53_002314"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HAQ4906106.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            7981..8505
                     /gene="atpF"
                     /locus_tag="IXT53_002315"
     CDS             7981..8505
                     /gene="atpF"
                     /locus_tag="IXT53_002315"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HAQ4906107.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            8492..9034
                     /locus_tag="IXT53_002316"
     CDS             8492..9034
                     /locus_tag="IXT53_002316"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HAQ4906108.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            9057..10613
                     /locus_tag="IXT53_002317"
     CDS             9057..10613
                     /locus_tag="IXT53_002317"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HAQ4906109.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            10627..11529
                     /locus_tag="IXT53_002318"
     CDS             10627..11529
                     /locus_tag="IXT53_002318"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HAQ4906110.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            11552..12958
                     /gene="atpD"
                     /locus_tag="IXT53_002319"
     CDS             11552..12958
                     /gene="atpD"
                     /locus_tag="IXT53_002319"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HAQ4906111.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            12972..13394
                     /locus_tag="IXT53_002320"
     CDS             12972..13394
                     /locus_tag="IXT53_002320"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HAQ4906112.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
BASE COUNT         4530 a         2450 c         2988 g         3732 t
ORIGIN      
        1 tttcggaaaa aagacgagta tctacttttg tcataatttt actgttagca ggtttctgcc
       61 aaaattcata acgatcaacc aatgtttgtc cacgacttat tccctgagtt tccacagcgg
      121 tgaagctttt gaaaccttca cagatggccc cctcgatgaa ataagcaaca gctaacggat
      181 cattgatgac acatcctaaa attctttctt gtttccagtg gaagtcaaaa taaaaacgag
      241 tgatcgcttt taaatactct ccctcttcag gattcatttg acagcagtat tcgaggatag
      301 tcggtgtcaa gacgatttct cttgtcacat ctaacccaac catttctatc gtttgtttga
      361 gattctcgaa tacataagaa gctgcttcag gatcacacca gtaattgtat tcagcaactg
      421 gcgaacagtt accatggctt ttgtaagttc cacccatcga gacaaaacga tccatatgtt
      481 tcccaatatt tgggttgact ttcaaagcaa aagcgatatt ggtcaaagga cccaaagcaa
      541 tgactgatgt atttgtcttt tctttgaaat aatcagccaa aaaatcaacg gcatggagag
      601 gttctgcctg ttttctcaag atcatcggga aatttgtttc ccctaaacca tccataccat
      661 gagtgtcttg tgcactgatg aaaggttgct tcaaaggttt ttccattcct tgataaacag
      721 gaatgtctaa acgatcacag cgttccaggc atttcagcac attctgcgta cctatatctg
      781 cagggacatt cccacaaact acggtaatag caactacttc gatatcaggg gatttgacag
      841 ctaatgataa agctaaagtg tcgtcgatcc ctggatcgca atcaataatt acttttcgca
      901 ttatttccaa tctcctttct ttagagatac ttagtttttt attctgggaa gtttccgaac
      961 cagtccaaga cgagcgtctt gattataatg cacgaaatta gtatactggc ttttattgcg
     1021 aatgtaaatg ctctcttaca agtaaatcta ttgaggagaa ctttaagtaa atgatacaat
     1081 gaaaaagaaa tggaatttac gtgtgccgaa aataaagaat cctatggatc ggtacattgg
     1141 ataatagata gattatagtt tggcagaaag cggcgggtgt acaatggtta ttttttattt
     1201 cattcttatt ctgataatta tagggatcag ttaccatttt ctttcttatt tttatgcaaa
     1261 gaaattagta agagcgggag tacagagagg atatgactgg tatgaagaca ctggtcatcg
     1321 tttgatggac cctgctgcag tcacgtcagt agaaaaaaaa cgacaaaaat tagaagaaga
     1381 gtgtgaaagc ttttgggagc agggaacacc caaaacaatc aaaagttatg acgggttgaa
     1441 actagctggt cagatgttca tccatccaaa taagcagaat aaatgggtca tttgcgtaca
     1501 tgattaccga agcacaggga aaagagacat gtctcatata ggtaaaagat atgcagagaa
     1561 aggattcaac gttcttgtac ctgatttacg tgcgcacgga gaaagtgaag gagaaatcat
     1621 cggaatggga tggctagatc ggctagattt aattgcctgg atccaactca tcttagatga
     1681 acagccgaat gcgtccatta ttttacacgg cggatcaatg ggagcttcta cgatcatgat
     1741 ggcaagtggt gaaaaacttc catcagcggt caaaggattt attttagaca gcggatatgt
     1801 ttctgtttat gcagaattcc gatatatgtt aagtaaaatc actgtctttc ctaaaaaaat
     1861 ggtcatgaga tatgcgaatc attatgccca gaaatacgcc ggttactcct taaagcaagc
     1921 atcagctacg agacagcttg gcagcaacca tctgccttta ttagtcatcc atggtgaaag
     1981 agatcatttt gtccctacag aagcagcgta cacgattcaa aatgctacgg caggggataa
     2041 agcactgctg cttgttccag aagcagaaca cttggaagca tcgatgaaag atccgaaaac
     2101 ctattggaca gtgatttttt catttattga acaaagaata acgatggatt aattttctct
     2161 tcttttttgt aagtaattcg aggataaata attgtttata gatctggaga ctgctaaaat
     2221 aaggtaggaa taaaaaaata tcagttattt gaggagtgcg gcatatggaa atcaaagaag
     2281 aaaaaaatcg tcttgcttta ttcaatgatg agcaacaaga gattggagaa atgacatggt
     2341 cagatgcggg cccagacatc atgatcattg accatacatt tgtagatcca gcatatcgtg
     2401 gtcaaaagtt agctgaaaag ctagtgtata ctggagtaga attagcaaga cgagacggga
     2461 aaaagatcat tcctttatgc ccttatgcaa aaaaagagtt tgaaaagaaa ccagaatatc
     2521 aagacgtctt acgtcagtca taagaataac agctgaaagt tttgaacttt cagctgttat
     2581 tccatttata tagagttgtc acggactttt atctatgata aagttaaaga gacgatatct
     2641 tttgggaagg aggctgcgat atgtataatt taattttggg aatcgtgatc gttttatcga
     2701 ttgttatggt cattgcgatt atgatgcagc caagcaagca aaatagtgca gcgagtgcat
     2761 ttaccggcgg tgcggatcaa ctattcggca aacaaaaagc tcgcggcttt gaagctgtca
     2821 tgcagcgatc aactgcagtt atgggagcta tctggatgat cttattattt gtcctagcat
     2881 atttatcttc gaagtagttg aataagcctc tcgcaaaacg gcgggcggct ttttttattg
     2941 caaagagaac gcttaagaag aagttatgaa agaaactatg gtaaaatgag gcgaaagagg
     3001 tgagacgagt gagacaatta ccaaaacccc tttatgcaaa acatggaaag cgagcggttt
     3061 tgctgcttca cgcttactca ggaagtccaa acgatgttcg aatgctcgca cgatttttgg
     3121 aaaaagcaga ttatacagtt tatgcgccgc tttttaaagg acacggaaca atggagccat
     3181 atgatatctt gcaagaaaaa gcagaaagct ggtgggcaga cactaaaaaa gccatccatt
     3241 ttttacagtc tgagcagttt tctgatattg ccgtattagg attatcaatg ggcggcatct
     3301 ttgctgtacg agcactagaa gaagaatcag tgattggcgg cggttttttc tgttcgcctt
     3361 tgtctcctgt aaaaacaaac gtgcctgaaa actttgaaaa atacgtacgt caagtgctga
     3421 agacagcagg taaatcagag aaagaaatca atgaaaaagc agttgcttac cgctcgttag
     3481 ctgaacaaca attaatggat atacaagatc aggcagcaat cgtagaaagt aggttgtcag
     3541 atatccaaca gccgatattt ttagctcaag ctgggaaaga tgaaatgatc gaccctaatg
     3601 gcgtatttga aactgcacgc aagttgagcc gtcagcgagt gacacttcaa tggtatcctg
     3661 aaagtggtca tgtgattaca gttggtacag caagacgcga attagaaaaa gacgtcttgg
     3721 aatttttaga aaaattacct tggaatgagg aataaatatg acaaaagaaa cgataaaaga
     3781 gaaaattctc tttttcatgg aaagcagtcg caaacgcagt ttttctatgg aagaaatcgc
     3841 tgaaggttta gggttccaaa aaagcgatga ttttaaatta ctagtacaaa cgattgctca
     3901 aatggaaaga gaacagagta tagttttcaa taaaaaagga aaagtgaaac ttcctttaaa
     3961 accaatcttg atcgaaggaa catttcgagc aaatgaacga ggatttggat ttgtaacgat
     4021 tgatcccgaa gaagatgaca tctatatccc aaaagaagcg actggttttg caatggatgg
     4081 agacaccgta gcaatcgata tcatgaagcg agcaaataca gatatggatc gcggagcaga
     4141 ggggaaagta gtcgagattc gagaccgggc gactactcag ttagcagggg aattcgttgc
     4201 ttattcagaa gaagaaatac aagaaacaga tctttacggc gttgttatcc cgaaagatag
     4261 aaagctcaac caatttaaag tatatgcagc agcagaggga gttcgtccag ttgacggaag
     4321 tattgtgctg atcgaactga cccattaccc tgaaaaaggg tatgcgacta gtttagaagg
     4381 gataatcaag caagtgattg gtcataagaa tgaccctggc atggatatcc tctcaattgt
     4441 cgtggctaat ggtatcccta cgaaatttcc tgatgatgta ttgtctgaag cagatgaagt
     4501 tccagatcaa atcagcgaaa aggatataac tggacgtaga gatttaagaa atcagctcat
     4561 tgttacgatt gatggagaag atgcaaaaga tctagatgat gcagttacgg ttaaaaaatt
     4621 agataacggt aattattttc ttggagtaca cattgcagat gtttcttact atgtgacaca
     4681 aggcagtcaa ttggacagag aagcatatga aagagggaca agtgtatatt tgactgatcg
     4741 agtgattccg atgatcccgc aacgtttatc gaatggtatc tgttcactga atccccatgt
     4801 tccaaggctg accatgagtt gcgaaatgga gattgatcca aatggacaag tagtctctca
     4861 tgatattttc ccaagtgtga tccaaacaac agaacggatg acctacacag ctgtcaatca
     4921 aatcttagaa gatcaagacg aagaaacgat gcaacgctat gctcagctcg tacctatgtt
     4981 ccaagaaatg ggtgaattgc atcgaatcct tgaaacgatg cgaatcaaac gtggcgcgat
     5041 ttcttttgag gatcgcgaag cgaaaatcct tgttgatgct gaaggacacc caaatgacat
     5101 ccagctaaga agcagaggaa ttggtgaacg cttgatcgaa tctttcatgc ttgcggccaa
     5161 tgaaacggta gctgaacatt ataataaatt aaatctgccg tttatttacc gaatccatga
     5221 acagccaaaa gaagaaaaaa tgcagcgctt ctttgatttt gcttcagctt tgggcattct
     5281 ggtccgagga acgaaaggaa cgatcacacc aaaagattta cagcatgtga tcgaaggagt
     5341 agaagacaaa ccagaagctg cagttatcaa tactatgttg ctaagaagca tgcaacaagc
     5401 acgctattca gaagacaatt ttgggcatta tggcttagcg gcagattact acacacactt
     5461 tacatctcct attcgacgct atcccgatct aatcgtccat cgtctgatcc gttcttacag
     5521 tgaagataaa agtgaaagca atcaaagttc gtgggctgcg gaacttcctg aaattgcaga
     5581 tcatagttct aagatggaac gtcgtgcagt agaaacagaa cgtgaagtag attccatgaa
     5641 gaaagcagaa ttcatgatgg acaaagtggg agaagaattc gatgggatca tcagctctgt
     5701 tgtgaaattc ggcctgttta tcgaactgcc gaatacagta gaaggattga tccatatcaa
     5761 tgaattgaaa caggactact tccattttgt tgaaaatcat ttagctcttg taggtgaaag
     5821 aactggtctg acttttaaaa tcggacaaaa agtgcgcgta aaagtagtca aagctgatcc
     5881 agatgaacga gcagtggatt ttgaactcgt ttccgcagaa gaggtcactg cgttggaacg
     5941 tccaaaagca cgcaatggaa aatttggaaa acgagaagga aaacaagcag gcaaacgctc
     6001 agaaaaaaat ggcaagaaga acaaacgata tagtgatagt aagcaaacgt tcggtaagaa
     6061 aaaagggaaa aagccatttt acaaagatgt caaaaagaag aaaaagaaaa aataaaatga
     6121 gttggaggaa acagctatgc caaaaggcga gggaaaacta attgcacaaa acaagaaagc
     6181 tcgccatgat tattcgatca tcgacacgat ggaagcaggg atggtcttgc aaggaacgga
     6241 gatcaagtcg atacgaaaca gccggatcaa tctgaaagat ggatttattc gcgtccgcaa
     6301 cggggaagct ttcttgcata atgttcatat cagtccttat gaacaaggaa atatttttaa
     6361 tcatgatccg ttgcgcacga gaaagttatt attacacaag aaacaaatcg ttcgtcttga
     6421 aaacgaattg aaaaacactg gaatcactgt tgttccttta aaagtctata ttcgtaacgg
     6481 ctatgccaag gtattgattg gtctggcaaa agggaaaaaa tcgtatgata aacgggaaga
     6541 tttgaaacga aaagatatcg atcgacaaat tgatcgaaca ttaaaaaatt tctctagata
     6601 atttcattat cctttttcgc atattcggtg cttttgcttt tacttaatga taaaatttgt
     6661 tagcatttaa tctgataaaa catgattatc acaggttttt tcttagatca ggagcaccgt
     6721 gcatggggaa aaattcgctt cgtttttgac tgaaataaaa aagtgtctaa ttttcatgaa
     6781 aatgttttga attttctgtt tcatggtggt atgataccgt gtggttagaa accgattgga
     6841 aaaaaccaga tacgcacatg aattgatttt atgtgaattt cggggaaaag aggtgaaatg
     6901 gattggatga acgctcgcta acgtttcaca ttggaccaat ttggtttgat ggtacagttt
     6961 gtataatggt gttactgacc tgtttgattg ttttcttctt agcttacttt tttacaagaa
     7021 atcttaagtt gaaaccaaca ggcaaacaaa atgcgataga atgggtcatt gattttagtc
     7081 gaggaatcgt aacagataat ctgcctagaa aagaattgag caatttccat ttgttggcat
     7141 ttacattgtt tttatttgtt tttgttgcaa acaacatagg tttagtaaca aaaatcgtct
     7201 tacctaacga aacaacactt tggaaaagcc cgacagcaga cccgtttgtt actttaactt
     7261 tggcatttat aatgatctta cttactcact tattcggggt gaaaaagtta ggcttcaaag
     7321 gatatttcgt aaattcgttc ttgaaaccct atagttttat gtttccgatg aaactgattg
     7381 aagaatttac aaatttatta acacttgctt tacgtcttta tgggaatatt tatgcggggg
     7441 aagttttgct aaccttgatt gcaaaaatga tgacaagctt aggctggttc tcattaccat
     7501 tagcaattcc attagagatg gtctggatcg cgttctcctt gtttattggg agcatccaag
     7561 catttgtctt tgtcactttg tcaatggttt atatgagcca taagattgaa gtagaagaat
     7621 aatactcggt taaaaaacaa actactattt atctatttta ggaggaaaac aattatgaat
     7681 tatatcgcag cagcaatcgc aatcatggga gcagcaatcg gagccggtta tggtaacggt
     7741 caagttatct ctaaaacaat cgaatcaatg gctcgccaac ctgaaatgtc tggtcaattc
     7801 cgtacaacaa tgtttatcgg ggtagctcta gttgaagctg ttcctatctt aggggtagtt
     7861 atcgccttga ttctagtttt tgccgtttaa ttgaatcagt attaaggcag aaacaacatc
     7921 gtttgttact gcttttttac atggaaacta gaaatactcc aaagaaagga aggctcgcct
     7981 atgctgaatc aattggcaat tgcagaagtt ggcaatgcga tgttaggtaa tatcatcgtt
     8041 gtcagcggct catttttgat actactggct ctcttgaagc actttgcttg gggaccaatc
     8101 agcgatattt tgaaaaaacg tgaagacaag atcgccaatg atttagattc tgcagaacaa
     8161 tctcgcatca actcagcgaa aatggaacaa gaacgcgaac aacaattgtt agcctctcgt
     8221 tctgatgcag ctgatatcat caaaaatgcg aaagaaagtg gagaattaag ccgccaaaat
     8281 attttgaagg atgctcaaga agaagcagct cgtctaaaaa gcaaagccca agctgatatc
     8341 actgtagaac gtgattcagc gctgaactct gtaaaagacg acgttgcaga actctctctt
     8401 caaatcgcgg aaaaaatctt gaacaaagaa ttatctccag aaatgcatga atcattaatc
     8461 aatcaataca ttgaaggtct aggttcttca aatgaaacta gataaatata cagtaggaag
     8521 acgttacggg aaagctttat ttgaactggc gatcgattca aatcaagcag aagaaatcta
     8581 tcaagatctc gtaagtttac gtcaaatcta tgaacaagta cctggattag ggaatatgtt
     8641 aagtgatgtt cgtctagaac ctcatgaaaa acgagaaatc atggacaagc ttgtcagcgg
     8701 ttatgatggg atcgttaaaa actttttaga agttgtttat gagtataacc gaatggatga
     8761 ccttttgttc atgatcgaag aatatgaacg tcgatatgat gaacatcaag ggttactatt
     8821 gggaagtgtc acaacagcag tcccactttc agacgaacaa cgaatacaat tagaaaagaa
     8881 tgttgcaaaa acaatggact atcaatcagt tgaattaaaa caaatcgttg attcttctat
     8941 tattggtggc gcgattgttg aagccaacca tcgagtaatc gacggaagca ttcgcacgca
     9001 attagaaaaa atgcgtaatc aattaaacag atagtacaaa gaggtaggtg aatcgaatgg
     9061 ccatcaaagc agaagaaatt agtgccttga ttaaagaaca aattgaaaat tatcaaaacg
     9121 tgctttcagt cgaagaaatc ggtaccgtaa cttatgtagg tgacgggatc gctcgtgcac
     9181 atggtttaga aaacgcgatg agtggtgagt tgcttgaatt ttcaaacggc tcatacggaa
     9241 tggcgcaaaa cttagagtca aatgatgtag gtatcattat ccttggcgat ttcgagtcca
     9301 ttcgtgaagg agataaagta aaacgaacag gtaagatcat ggaagttcca gttggagagg
     9361 ccttgatcgg tcgggtagtc aatccgctag gtcaaccaat cgacggacta ggtgaaatcg
     9421 ttactgacaa agctcgtcca gtagaagcga tggcaccagg tgttatgcaa cgtaaatctg
     9481 ttaacgaacc aatgcaaaca gggctaaaag cgatcgacgc ccttgtgcca atcggacgcg
     9541 gacaacgtga attagtcatc ggtgaccgta aaacagggaa aacttctatt gcgatcgata
     9601 cgatcatcaa ccaaaaaggt caagatatga tctgtatcta tgtagcaatc ggacaaaaag
     9661 attctacagt tcgtacacaa gttgaaacat tgaaaaaata tggcgcaatg gattacacaa
     9721 tcgttgtgaa tgccggtgcg tctcaaccag caccattgct ttatatcgca ccatatgctg
     9781 gtactgcaat gggtgaagaa ttcatgtaca acggtaaaca tgtattgatc atttttgatg
     9841 atctttcaaa acaagctgtt gcttatcgtg aactttcctt gctgcttcgc cgtccaccag
     9901 gtcgtgaagc ttatccaggg gatgttttct acttacactc acgcttattg gaacgtgcag
     9961 caaaattaag tgatgattta ggtggcggtt caatgactgc cttaccattt gttgaaacac
    10021 aagctggaga tatctctgct tatatcccaa caaacgttat ttcgatcact gatggacaaa
    10081 tcttcttaga aagcgacttg ttctacgcag gtactcgtcc agcggttgat gccggactat
    10141 ctgtatctcg tgtagggggt tctgcgcaaa tcaaagcaat gaaaaaagtt gctgggactt
    10201 tacgtctaga cttagcaagt taccgtgaat tagaagcatt tacacaattc ggttctgact
    10261 tggatgcagc aacacaagca aaactaaatc gtggacgccg tacagtagaa atcttgaaac
    10321 aaaaattgca tgcaccgcta gctgttgaaa aacaagtagt gattttgtat gctttgacac
    10381 atggattctt agatagtatc cctgtagaca gtattttaga cttcgaacat gaattattcg
    10441 aatatctgga taccaatcat tcagatatct ttgaaacaat ccgtacaaca aaagatcttc
    10501 cagaagaaga agcgttgaac agtgcgattc aggaatataa agacatgttc ttagctacaa
    10561 aaggaaataa ttcctctaca gaagataaat tgaaatccat tcaaaatgca tagtgaggtg
    10621 agatgaatgg gtgcttcatt aaatgaaatc aagacacgta tcgcttcaac gaaaaaaacg
    10681 agccaaatca cgcgggcaat gcagatggtg tctgcttcta agttgacaaa atctgaagct
    10741 tcttcacaga agtttcaggt ttatgcaaca aaagtaagag aaatcgttac gcatttgact
    10801 gctactcagt taaatgatat tgcctcagaa aatcctagag gggatatcaa ttacaacagc
    10861 atgttgctca gccgtcctgt taagaaaacg ggttatatcg tcatcactgc agatggcggt
    10921 ttagtcggtg gatataacag ctcgatattg aaacaaacga tgtctatttt agaagaagac
    10981 caccaatcac ccgatgatta cgtgatgatc gcaattggcg gaactggtgc agacttcttt
    11041 aaagcacgcg gtatcaatct agcatatgag ctgcgcaatc tatctgatca gccaagcttt
    11101 gatgaagtcc gcaaaatcgt aaatatggca acaactatgt accaaaacga agtattcgat
    11161 gaattatacg tatgttataa ccatcatatc aattcgctaa ctagtcagtt tcgtgtagaa
    11221 aaaatgctgc caatctcaga cttagatcca gaagaagcaa ctactttcga acaagaatat
    11281 atttttgaac cttcaaaaga agaaatcttg tctcagttat tgcctcagta tgctgaaagt
    11341 ttgatctacg gtgcgatcgt tgacgccaaa acagcagaac atgctgctgg tatgacagca
    11401 atgaaaacag caactgataa tgctgctaat atcattgatg agctgacggt ttcttacaac
    11461 cgtgcaagac aaggggctat tacccaagaa attaccgaaa ttgttgccgg agcttcggca
    11521 ctagaatagt attgagattg gaggaaaaaa gatgagttca ggcaagattg ttcaagtaat
    11581 cggtcccgtt gtcgacgtgg aattttcttt agaccaatcc ttaccagata tcaacaacgc
    11641 attagttgtt tataaaaatg acgaaaataa atcaaaagtt gttcttgaag cagcgttaga
    11701 attaggggac ggagtgatcc ggaccattgc gatggaatca acagatggtt tacaaagagg
    11761 aatggaagtc attgatacag gcaaagcaat ctctgttcct gtaggtaaag aaacattagg
    11821 tcgcgtattc aacgtactag gagatacgat cgatttagaa acacctttcc cagaagatgc
    11881 ggaaagaagc gaaattcata aaaaagcacc agcctttgac gaattaagta caagtacaga
    11941 aattttggaa acagggatca aagttatcga tttgcttgcc ccatatttaa aaggtgggaa
    12001 agtcggacta ttcggtggtg ccggtgttgg taaaaccgta ctgatccaag aactgatcca
    12061 taatatcgcc caagaacatg gtggtatttc tgtatttacc ggtgtaggtg aacgtactcg
    12121 tgaaggtaat gacttgtatt atgaaatgaa agattcagga gttatcgaaa aaacagccat
    12181 ggtgttcgga caaatgaacg aaccaccagg tgcacgtatg cgtgttgctt tgactggttt
    12241 gacgattgcg gaatatttcc gtgatgtaga aggtcaagat gtactgttgt ttatcgacaa
    12301 cattttccgt ttcactcaag ctggatctga agtatcagcc ttgttaggac ggatgccttc
    12361 tgcggttggt tatcaaccaa cattggcaac agaaatgggt caattgcaag aacgtatcac
    12421 atctacgaaa aaaggttcga tcacatcgat ccaagcaatc tatgtgccag ccgatgacta
    12481 cactgacccg gcgccggcaa cagcattcgc ccatttggat gcgacaacca acttggaacg
    12541 taaattaaca gaacaaggga tttatccagc ggtagatcca ttggcttctt cttcaagtgc
    12601 tttagcacct gaaatcgtgg gagaagaaca ttataaagta gcaacagaag tccaacacgt
    12661 attgcaacga tatcgtgaat tacaagatat cattgctatc ttaggtatgg atgaattatc
    12721 cgatcaagaa aaagtgttag tttctcgtgc ccgcagagtc caattcttct tgtcacaaaa
    12781 ctttaacgtg gctgaacaat ttactggtct tccaggttca tatgtccctg tagaagaaac
    12841 agtgaaagga ttccgcgaaa tcttggaagg caaatacgat gatcttcctg aagaagcttt
    12901 ccgaagtgtt ggccgaatcg aagacgtcgt agaaaaagcg aaaacactag gctactagaa
    12961 aggggtgccc catggatcaa tatttaacag taaatgtggt aactcctgcc ggtttggtct
    13021 atgatcacca tgcagctatc gtcgttgcac gtacgacagc tggtgagctt gggattttgc
    13081 ctaaacatgc accaatcatc gttccattga cgattgatga agttcgtgta aaacgaacag
    13141 attcagatac ccacgtcgat tggatcgctg ttaatggcgg aatcatggaa gttcgtgata
    13201 atgttgtttc aatcgttgct gatagtgcgg aacgtgaacg tgatatcgat gtatctcgtg
    13261 cggaacgtgc aaaacaacgc gcagaacgcc aaatcgaaga agcaaaagca aaacaagata
    13321 cgaacgaatt gaaacgggca actgttgcgt tgcaccgagc aatcaatcgt atcaaagtat
    13381 ctaagcactc ataaaatgag atgcggatgt ttatccgtgt ttaagaacag taaaaagagg
    13441 ttatgacaga agtggtcagc ttcaagacat aagaaggaat ttacgaaaat tgcttctcaa
    13501 atttttgtga aatctcagct tatttttcga agaagctact tctggaacac cgtatatcgg
    13561 aggttgtgaa atcagcgttt tgtcctgagt attagagaaa caaatggaaa atagttcctc
    13621 atattttgcc gttttttgag ctaataccga gggtcagatg attgaacacc gtttatttgg
    13681 aataagggac tgcgacaaga
//
LOCUS       DACGLW010000082        13171 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.085, whole genome shotgun sequence.
ACCESSION   DACGLW010000082 DACGLW010000000
VERSION     DACGLW010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13171
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.085"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            complement(203..1654)
                     /gene="cls"
                     /locus_tag="IXT53_002321"
     CDS             complement(203..1654)
                     /gene="cls"
                     /locus_tag="IXT53_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HAQ4906113.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            1995..4661
                     /locus_tag="IXT53_002322"
     CDS             1995..4661
                     /locus_tag="IXT53_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HAQ4906114.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNFPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5025..5777
                     /locus_tag="IXT53_002323"
     CDS             5025..5777
                     /locus_tag="IXT53_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HAQ4906115.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(5799..6953)
                     /locus_tag="IXT53_002324"
     CDS             complement(5799..6953)
                     /locus_tag="IXT53_002324"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HAQ4906116.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            7138..9579
                     /locus_tag="IXT53_002325"
     CDS             7138..9579
                     /locus_tag="IXT53_002325"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HAQ4906117.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(9909..10613)
                     /locus_tag="IXT53_002326"
     CDS             complement(9909..10613)
                     /locus_tag="IXT53_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906118.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(10800..11045)
                     /locus_tag="IXT53_002327"
     CDS             complement(10800..11045)
                     /locus_tag="IXT53_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906119.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(11062..11913)
                     /locus_tag="IXT53_002328"
     CDS             complement(11062..11913)
                     /locus_tag="IXT53_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HAQ4906120.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            12079..13083
                     /locus_tag="IXT53_002329"
     CDS             12079..13083
                     /locus_tag="IXT53_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HAQ4906121.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
BASE COUNT         4202 a         2340 c         2750 g         3879 t
ORIGIN      
        1 gaaagaggtc gtgagcctgc cgtccagctc cgagtcacaa ggaacaagtt caaaaaacag
       61 cttctcatgt ttgttgaata ttgtgacttg tgaccgagga gttggcagga gaacaccgtt
      121 tgttcggaag aagggactgt gacaagattt ttgtcacagt ccctttaatc ttagtatatt
      181 tttctctact tgaataaaaa aattatagga ttggagaaaa taatcgagaa atttgttgct
      241 tgaataatag ccagttagac atttccttga ctgtttcagt tgtcatggat gaacaatgtg
      301 tctcatcttc tttgaactga gtagcaagtt cttctaaaaa ctcaggatcg taaatgacag
      361 cacttgtttc aaaattcaat cggtagctac gaatatcttg atttgctgaa cctaccatgc
      421 agatttcatc atccattatc aatgtttttg catggaggaa gccgttctca tagacaagga
      481 tttgtacatt ttccttgatc agctgccgag cataatattg tgttgctcga taaataaaag
      541 gatgatccgg tttgttggga atcatgattt tcacatctac accagaggca gttgcgattt
      601 ttaaagcagc aatgacactt tcatcaggaa caaggtaagg tgtctgtatc caaacacgtt
      661 ttttagcaga agtaatcaat ttgataaatg aaagcttgat ttgttccctt tcgttattcg
      721 gtccgcttga aacaagctgg atggatgtat cagcaagctt agattcatct cgatctgctt
      781 ttttaaagaa ataattcaaa tgatagccga cctttttttc ttcaggtacc gagacgttcc
      841 agtctgttaa gaagcgaagc tggagcaaag aagaagcggc accgaaaatc cgtatatgcg
      901 tatcgcgcca atagccgaat ttttttgttg tatttacata ttgattggca atattgaaac
      961 caccggtata actaatcttt ccatcgatca caacgatttt tcggtgatca tggtaattca
     1021 atcggaaacg aagtaatgcc ctttgtgagg taacaaatgt atggacatgt ccaccatttt
     1081 tgattagacg attgaaatct tttgcttttg tgccatgaga gccaaatgca tcatataata
     1141 ttcgaacttc cacgccttct gcggcttttt cttctaaaac atgtaagact tgctggccga
     1201 tattatctgt cacaaatgcg taatattcga tatggatcga atgtttggct ttttttagat
     1261 cttgaagcag tgaatccaat ttctcttgtc cgtctgtgag aagagtgaca gaattcattc
     1321 ttgtcagcgg catacgattt aatgaagaga agaagtcaac aaattgctgt ttgtctttat
     1381 cgcccatgtc aggatcgtaa tgttcaatgg tatctcctct aaaagactga aagttttcta
     1441 attctttcaa gtcactttgt cggagataga attttttctt atccgttaat ccacgtccga
     1501 aaaataaata taatacaaag cccaccccag gaagggcaaa caatactaag agccatgccc
     1561 aaatggctgc tacatctcgg ggtttgatca aaatagccac caaagcaata agtgcattta
     1621 atagataaag ggcggttata atactagata ccacaaaaat gttcctcctt acatacaata
     1681 acattagtat agcatgatag aagaaagaag cctaccaagg tgaatatact tttcactcct
     1741 ttcatgaaaa tgtaggtaaa tcgggctaaa gtagttgctt tttgtaggtt aataaggtag
     1801 gatattagta cagttgaata tctccttagg ggagtaaccg gcaacgtttg ttgcgataat
     1861 atcaacagta agttaaccaa atggttttct ggtattgtat gaaatggtga gacctaagac
     1921 aaatgcgtat ttgtcttagg tctctttttt ttcagacagg actagggcca agatcaaaaa
     1981 atgggggatg aaatttggaa aacaaggaac aagcacaaca agcggtctat aaagaaacga
     2041 tcgactcgtt gatgcagcaa acgcagagct caaccgatgg attgagcacc acgcaagcga
     2101 aggaaaaatt agaaagagat ggcttgaacc agctaaaaga aaagccgaag aagccaactt
     2161 ggaaattgtt cttagaaact ttcaaagatg caatggttat cgtattgctg attgtcgcac
     2221 tgattcagat ggtcatgggt gactacatcg aattcttagt tatttttgca gtattgatgt
     2281 tgaattctat aatcagtgtt gtccaaacaa aaaaagcaga aggttcttta gaagcgttga
     2341 aaaacctttc agcgcccgat gctcgagtga ttcgtgatgg taaggaaaaa acgattccgg
     2401 caaacgaact tgtggtagga gatatcgtgt tgttagacgc aggcgattat gtacctgctg
     2461 atggacgttt actagatgcg ggatcattaa aagtagatga aggtatgctg acaggagagt
     2521 cgacttctgc agaaaaagaa gtaacggata tcacaagtac ggtaccaatc ggagatcgta
     2581 caaatatggt attcagtgga acgatcgtaa cttacggacg cggtaaattc ttagtgactt
     2641 caactgggaa taatacggag atcggacaag ttgctggcct tttggaatca acaactgagc
     2701 aagtcactcc gttacaacga ggtctggata aattcagtaa aaaactaagt ttggctattt
     2761 tggcattgtc tttattgatt ttaggtatcc aactgtttcg tatttatctt ggagaaggta
     2821 caggagatat gactgcagat atcgtcagcg cagtgatgtt tgccgtagcc gtagccgtag
     2881 ctgctattcc agaagcatta cagtctatcg taacaatcgt gttatctttg ggaacgaata
     2941 aaatggcaaa acgtcatgcc attattcgga agcttccagc agttgaaaca cttggttcga
     3001 caagtatcat ctgtacagat aagactggta cattgacaca aaacaaaatg acagttgttg
     3061 actacttttt aggtaatggc gatactgggg atttcacaaa tgacccttca aaatggacag
     3121 ctgacgaacg tcgcttgatc gaaatcagtg ttttagcgaa tgatgcatcg atcagtgaag
     3181 acggtactaa attgggcgac cctacggaag tagcgtttat cgactttagt gaaaaattaa
     3241 accagcctta tcaggaaatc cgaaacaact tcccaagaca agcagaactt ccttttgact
     3301 cagatcgaaa attaatgtct actggacaca caattgataa caaaaattac ttgtttgtta
     3361 aaggcggacc agatatcgtc ttccaacgca gtaaaaaagt attgctaaat ggtgaagtcg
     3421 ttccgatgac ggatgaattg atgaaaaaat tccaagagca aaatgaagct ttttcaacac
     3481 gcgcattacg tgtactagct tttgcttacc gtccacttga taattttgat ttatcatttg
     3541 aagatgaaga cgatttgatc cttgttggtt tgcttgctat gatcgatcct ccacgtgctg
     3601 aagtgactca ggcggtaaca gaagcaagaa gtgcaggaat caaaacaatc atgattactg
     3661 gtgaccacaa aacaacagct gttgctatcg caaaacaaat cgggattttt gaagaaggaa
     3721 acctggcttt gaccgggacg gaactagatg aattgacaga agatcaacta aacgaagttt
     3781 tagaaaaagt cactgtttat gcacgtgttt cacctgagaa taaaattcgt atcgttcgtg
     3841 cttggcaaaa taaaggtcat atctcagcta tgacaggtga tggtgtaaat gacgcaccag
     3901 cattgaaaca agcagacatc ggaattgcta tgggaacggg tacagatgtg gcaaaagatg
     3961 cagcagcgat ggtattgacg gatgataact ttgcttctat tgtcagcgca gtagaagtag
     4021 gacgtaatgt gtttgataat atcaagaaag ctatttctta tctatttgca ggaaatttag
     4081 gtgcgatcat tgcgatcatc tttgccttag ttgctgactg gtccaatcca tttacagcat
     4141 tacaactgtt gttcatcaat ttagtcaatg attcactgcc agcaattgca ttaggtacag
     4201 aaaaagctga acctgctacg atgaaccgcc cgccaagaga tccaaattca ggaatcttta
     4261 cggacaaaac attgatttct gtaacgtatc gtggtgtgct gattgggatt gctgttatct
     4321 ttgctcaatg gcttggcatg cagcattcac ctgaattcgg tgtagccatg tcattttcaa
     4381 cattgatttg gtgtcggaca ttgcaaacat tcccagcacg ttcaaacaca caaacagcta
     4441 tccaagcagg gttcttctct aacagaagtg tcttactagc tgtagttgta tgtagcgcgc
     4501 tttactgctt aacaatgatc ccaggactga aagaagtctt ctctatccca tcagcatttg
     4561 gattgacaga agtctttaca tgtatgggaa tcgcacttgt gacaattgtt ttgatggaaa
     4621 tcactaaatt gatcattgta cgagtacaag gaaataaata aaacgaatca acgaggttgt
     4681 gagcctgccg tttaattctg agtcacaagg tgcaatttca acaaacagct tctcatgttt
     4741 gttgaatatt gtgacttgtg accgaagagt tggcaggaga acaccgttta aggaagaagt
     4801 tgtaaaatca gcattttgag ctaatactga gggtcatctg attggacact gtttattcgg
     4861 aaagagggac tttgacacga ctttggtcac agtccctttt tttatttatg atagacggaa
     4921 agccaatcat tttattattc atcaaaagag atagaataga taaaagagga gaatgattta
     4981 ttccgaaaag tgaaaacata taacaaaaaa ttagaggaaa tcaaatgaat gaaaaagaat
     5041 gggatgaatt tgctgaagaa tattatgaaa tccagcaaga atcgcaagta actattgctg
     5101 aagatgtaaa aaattatctg aaaacggaga aagtcctgcc tgctgaaaca gcagcagacg
     5161 ttgctggagg atctggcaga tttctttcct tagcaaaaga aattaatcgc tatgatttga
     5221 tcgatttttc ttcaggaatg ctagaaaatg caaaaaaaga agcagaaaaa aatgagttgc
     5281 caaatgtttc ctttatcaaa cagtcgttag aagaatttat ccaatcaaat gagacatatg
     5341 aaatggtttt tacagcagca aatccagcat tggtacatct tattcaatta gaaaaattga
     5401 tgaaaaagtc aagcaaatgg tgcgtgataa tcagagtagt ggaatcaatc gatgatgttt
     5461 tttatcctat cgaaaagcaa ctaggaatga tagaagaaga tcctaataca tcgccagttg
     5521 taatggatca atttgaaaca tacttaattg atgaaggatt caaagtgaag aaaaaagatt
     5581 ttacttattt tacacaggaa tcaattactg aagaattgat gaagctatac tatgacgcgt
     5641 acgcaaaaga tcctgttgtg accaggtaca ttcgcaaact attcagtgaa aaacgagaaa
     5701 tccttagtac gactaagctg acctatagac tgctcctttg gtccgcagat aaaaaaagaa
     5761 cctttcacga ctcgtgaagg gttcttttta tgattttatt atattttgta gtaacgaata
     5821 tcattttcga tctttgtaat cgaaaaaggt acatctgaag tatattctgc acattctccg
     5881 tgttcaggaa gcgtctcact gaaaatcgtt tcatattcgt caacagtgat ccgtgttcga
     5941 gaatctagca tctcttgatg agattgtgcg aagagatatt cttgatagtt ttcttccaga
     6001 taacctgtaa aaaattcact tactgcacca gaaccgtagc tgaatagacc gatgcgatct
     6061 cctggctgta gtgatttcga attttccagt agagaagtta gccccaggta taatgaacca
     6121 gtgtaaagat taccgattcg tcggctgtaa cggatgcttt cttcatagcg agccataaga
     6181 cgttcctgat tttcttcgtc tgtttggtct aatacgcttt gcaatgcctt ttttcccatc
     6241 ttagtatacg gaatatggaa agcaatcgcc tgataatctt cgagtcctcg acccgacaat
     6301 tctttatgtc gattccaaac tttttggaat gattcgatat acgtagaatt agataaagga
     6361 ccatcaacaa caggaaattc gctataatct ggacgccaga aatcatagat atcttctgtc
     6421 agaaatacgc tgtcgtcttc aatcgataaa atacgcgggt tttgagtgat catcatcgca
     6481 acagcaccga caccttgcgt cacttcacca ccgcttgcca agccgtaacg agcaatatca
     6541 cttgctatga ctagtacttt tcgttctgga tgatttttga catattcttt cgccatgtgc
     6601 aaagctgcgg tgccaccgta gcaagcctct tttacttcaa aagaacgagc aaaagattgg
     6661 attttcagta aatggtggat aattaccgca ctcgcttttg aatgatcaat cccggattct
     6721 gttccgacga tgaccatgtc gattagctca cggtcttttt ctgttacgat tttgctggca
     6781 gcatttgctc ctagtgtaat gatgtcttca tttgcacgat tgactgccat ttggtcttga
     6841 ccaatcccta tatggtattt tgcaggatca tccccacggc tttctgccaa ctctgtcata
     6901 tctaaatata aattaggaat aaaaaaggaa agacgatcaa tccctatttt catttcaaca
     6961 cctctttact tatgataaaa aacttacttt actaaggtat cataaaaaga ttaaaagaaa
     7021 tataaaaaag agaatattta aggattgtta aaatttaatc ttagaaaaaa ataactggaa
     7081 caagagctat ttgtttttac agcaaagaat ggtatatttt tgtaggaggt ggcttacttg
     7141 aaagaagtcg ttatgataga tgctgcaaga acgccgatag gaaagtatcg cggaagttta
     7201 agtccattta ctgcagttga attaggcaca cttgtgacta aaggattatt agataaaaca
     7261 aaactgaaaa aagataaaat cgatcaagta atatttggaa atgtcctgca agctggtaat
     7321 ggtcaaaatg ttgccagaca gattgctttg aacagcggtc tccctgtaga tgtcccagca
     7381 atgacgatca atgaagtttg cggatctggt atgaaagcag tgatcttagc acgccagctg
     7441 atccaattag gtgaagcaga acttgtgata gccggcggaa cagagagtat gtctcaagca
     7501 cctatgctga aacagtatca gtcagaaaca aatgaatatg gtgaaccaat ttccagtatg
     7561 gtcaacgacg gattgactga cgcattttca aatgcacata tgggattaac cgcagagaat
     7621 gttgcaacac aattttctgt gagcagagaa gagcaggatc gctatgcctt gtcgtcccag
     7681 ttgaaagcag cacatgctgt cgaagccggt gtattttctg aggagatcat cccagtcaag
     7741 atttctgatg aagacgtgtt atctgaggat gaagcagttc gtggaaatag tacattggaa
     7801 aaactgggca cgttacgtac agtattctca gaagaaggaa ctgtaacagc aggaaatgct
     7861 tccccgttga atgacggtgc ctctgtggtg atccttgcat ccaaagaata cgcagaaaat
     7921 aataatctgc cttatttagc aaccatcaaa gaagtagcgg aggtcggtat tgacccaagt
     7981 atcatgggaa tcgctccaat caaagccatt caaaaattga cagatagatc aggaatgaat
     8041 ttatcaacaa tcgatctttt tgagatcaat gaagcatttg cagcttcttc tatcgtagtt
     8101 agccaagaac tgcagttaga tgaagaaaag gtcaatatat atggaggagc aatcgcatta
     8161 ggccatccaa ttggtgcaag cggtgcacgt atcttgacta cattagctta cggattgctt
     8221 cgtgaacaga agagatacgg tattgcttca ttatgtattg gtggaggttt aggcttggca
     8281 gtcttgttag aagcgaatat ggagcaaaca cataaggatg tgcaaaaaaa aaagttttat
     8341 cagcttacgc catctgaacg gcgcagtcaa ttaatagaaa aaaatgtatt gacacaagaa
     8401 actgcgttga tttttcagga acaaacactt tctgaagagc tctccgatca catgatcgaa
     8461 aaccaagtga gtgaagtgga aatcccaatg gggatcgcgc agaattttca aatcaatggc
     8521 aagaaaaaat ggattccgat ggcaacagag gaaccatctg tcattgcagc tgcaagtaat
     8581 ggtgcaaaaa tctgtggaaa tatctgcgca gaaacacctc agcggctgat gcgagggcaa
     8641 atcgttctat caggaaaatc ggagtatcaa gcagtgatca atgcagttaa tcatcgaaaa
     8701 gaagagctga ttctttgtgc aaatgaaagt tatccttcaa tcgtgaaacg cgggggcggt
     8761 gttcaagata tctctactcg ggaattcatg gggagtttcc atgcatactt atctattgat
     8821 tttttagtgg atgtaaaaga tgcaatgggc gctaacatga tcaactcgat tcttgagagt
     8881 gtggcaaaca agttacggga gtggttccct gaagaagaga ttctattcag tattttaagt
     8941 aattttgcta cagaatcatt ggcttctgcc tgttgtgaaa tcccattcga acgcttaggg
     9001 agaaacaaag aaatcggtga acaaattgcc aaaaaaatcc aacaggcagg ggaatatgcg
     9061 aagcttgacc cttaccgtgc agcaacgcac aacaaaggga tcatgaatgg tatagaagct
     9121 gtagtggcag ctacaggaaa tgatacacgt gcggttagcg cttcgatcca cgcctacgcg
     9181 gcaagaaatg gattatacca aggattaaca gactggcaga tcaaagggga taaactagtt
     9241 ggaaaactaa cggtgccttt ggctgttgcc acagtaggcg gagcttccaa tatcctgcca
     9301 aaagcaaaag catctttagc tatgttagat attgattcag ccaaggaact agcacaagta
     9361 attgctgcag tagggttagc tcaaaacctg gctgctttgc gtgcattagt tacagaagga
     9421 atccaaaaag gccacatggg acttcaagct cgttcattag cgatctcaat tggggctatt
     9481 ggtgaggaaa tcgagcaagt tgcgaaaaaa ttacgtgaag cagaaaagat gaaccagcag
     9541 acagctatac aaatattgga aaaaatccgg gaaaaataaa aaagcaatat ctttagaaga
     9601 ataaagcaag atttacagtt attttattta ctgcagatct tgcttttttc tctatcagaa
     9661 aatatgttta ttattagaat tttttatagt taaggttagg catcttaaga aatcaggcaa
     9721 ctccaagata tcttcaggta gaagagaagt cgcctaacat tgtaggatca taggtgattt
     9781 gttggacagg tcaagctgat atttgagcct gatgttagaa ggaatttctt tttacttgct
     9841 gtagaaatta ttatatgact aaaaaggctg ggattttaaa tcccagcctt ttatgacaaa
     9901 tagaattttt atcgatcagt aatcgctgtc gctgcaaatt gttcgatcaa aggaatcaaa
     9961 gtcaatttga ctttgtattt ccgttcgagt gctttttcga taacgaactc attttcagta
    10021 gtcatagaaa tagttgctaa tttacgaggt tcatcagcat ttttttcatt aataactact
    10081 tcatgttcat tttgttctgt gtaaaattta ggatactcag caacggcttt tgaaaaatca
    10141 gcaaaactta aagcggcatt cgtatcagtt gagcgtgtat tttcagttgt gtccgctttt
    10201 ggtttttccg ttttttcttc tggcacactg actgtttctt ctgtaactgg tgtcgcaaca
    10261 gcttgcgcag ttggttcatc gtttttcaat cgttgatcat tccagtaaga cacgatttca
    10321 ataagaagat tgtcacggaa tcgttcgaaa atcccgtttt ctcctgaacg aattctcttt
    10381 aatacagtat tgaagttgtc atcgattggc acgttgatcc gtgtatcttc atttgttttc
    10441 ccagctaatc cgatcgttaa gatttgacgg tttttgggat caagcatgac ttgtaacggc
    10501 aaaccataac gtcgcaagct ctcttcttct agagaacgga ataattgtcc tcttacacca
    10561 tccaatgaag gacggaattg atttaataat ttttgtttct gttttttctt catatgttct
    10621 ctccttcgct catacccttt cgattataag aaaaaaacag ccaaatgaaa aggagtaatg
    10681 caaaaaaact tgcgtatacg caaattacaa cagaaaaatt tgcgtatttt cccgtttttt
    10741 gaaagaaaaa taaaaaatgg aacggattat gtatatatac ataatccgtt ccattctttc
    10801 tattttttcg gtatcaaggc aaaaacaggt agttcaggtg aataaatgac ttcatgttcg
    10861 tgccagggtt tgaccattag cgaatgatcg aaccgttttt tcagttcttc gatgatttga
    10921 tcatgtgtcc agtttcttgc aggaaaatgt tggattttct ctgcatcgat caaaaagaca
    10981 aaatcggaat gaaaagtctg ggtaacttct tccgagagag aattcaagcg ttcgattgga
    11041 tccataaaat cacctttcga cttaaaattg ggtagtatct gggtcttttg ctttgccgac
    11101 tacaccatct aactgatcga tttgtttcat ttcttcttct gttaattcaa aatcaaataa
    11161 ttcagtattt tctttgattc tgcttggtgt cactgactta ggaagaggaa ggaattcgtg
    11221 ttgcagcgac caacgcaaag caacttgtgc aatcgtcttg tcatgtgctt cagcgatttg
    11281 tttcatttca ggcacatcga agattttacc agtgcctaat ggactatatg cttcgaggat
    11341 catatcatgt ttttttgcat actcaactac agttgtctgt aattcgcctg gtgcaaggaa
    11401 aacttgattg accatcggca taatttttgc tgtttttgct agtgtatcta gatggtgggg
    11461 taagaaattg ctcacaccga ttgctttgat tttacctgct tcatacagtt cttcaaatgc
    11521 tcgccatgta tctgcatttg cttgttccca attatcacga aaagcaacag ggtttggcca
    11581 gtggatcaaa aacagatcta aataatctgt ttggagcttc ttcaatgatt cttcaaaaga
    11641 agacatcaca agttcatagc tgtggtttgc attccataat tttgttgtca agaatatctc
    11701 ttctctagga attccacttt ccttaatcgc ttggccgacg ctttcttcat tcttgtatcc
    11761 ttgggcagta tcgatatggc gataacctgc agccagcgct tctttaacag aggaaacagc
    11821 gacatcccca tcaggtgttt gccatgtgcc gaatcctact accgggattt cgtaaccatt
    11881 tgatagacga taagtgcttg ttaatgaatt catactttca gctccttttt tattcgttca
    11941 actataagtg tagcgcattt ttcgaaaaaa agaatccatt ttgtttctac tcttgaaatt
    12001 tttgtttaaa agaaaatgat tttgattctg gtatttttga ttttttttcc gtaaactaag
    12061 acagaaggga atgagaaaat ggatatctat ttaaaaaaag caattctgca tattattgac
    12121 agagaaactg gttctccagt cttttcacaa aaagagctgg atctgacaaa agaatatatt
    12181 cgtgattttc ttcaaaagaa gatccaaaaa atctcttctg ctcaaacaaa aactggacaa
    12241 ctagcagaag aaagcatgat cgttgaatca cttaaaatcg ctcaagaaaa ttttgtggaa
    12301 gcaagtgaag caatcgttca gcggtggttc gatatctacc aagaaagtga agaagcacct
    12361 agtgcagatg tatttgttgt cttgtatgaa ttagatacag tgatgtactt atctttattg
    12421 aaagtcaatt atcgtgaggc ttacactcac tttgttgaag cagacgaagc agggatcgac
    12481 aaccgcttga ttttgaatcg agcaattcta ggggcgaaaa cacaaaaagc agatgaagca
    12541 ttcgctgtga atttgactga ttggacctac gaattaattg aaaaaaaata tgagttttct
    12601 ggcaaaaaag agctttattt ttctggaaga gtgatcgaaa gtgttcctgc accttcttta
    12661 gaagaaaatg taaaagtgat ccaaaaagta gccaagcatc ttggaaaaaa atttgagaca
    12721 gaagaatttg atatcatggc tgatttgaaa gaagctgtct atgatacgat agaagaaaaa
    12781 ggtcggttgg atcatgagat gattgcggaa aaagtcttca aggaaaacat cacggcaaaa
    12841 atggctttta aagaagaagt acaagagcaa ggatttgtcc cagaagcacc tcctgtaaaa
    12901 gaagtacaag aaatttctga gaaaaaattt ggaaaacaaa aattcaagct atcaaacggg
    12961 atcgaactga tcgtaccagt agatgtatat cgcaacccag atttgattga atttgtgaat
    13021 aatccggatg gtacgatatc cgtgatgatc aaaaatgtgg atgaagtatt gaatcgttta
    13081 taaaaatgac tagaatatta ggtgttcaag taccagtgat cagttaaatg aattgtgtca
    13141 agagaaggat cggaaaagac gaatataaca a
//
LOCUS       DACGLW010000083        13146 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.086, whole genome shotgun sequence.
ACCESSION   DACGLW010000083 DACGLW010000000
VERSION     DACGLW010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13146
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.086"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            complement(77..149)
                     /locus_tag="IXT53_002330"
     tRNA            complement(77..149)
                     /locus_tag="IXT53_002330"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(114..116),aa:Lys,seq:ctt)
     gene            530..1099
                     /locus_tag="IXT53_002331"
     CDS             530..1099
                     /locus_tag="IXT53_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HAQ4906122.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1086..1946
                     /locus_tag="IXT53_002332"
     CDS             1086..1946
                     /locus_tag="IXT53_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ4906123.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2136..2840
                     /locus_tag="IXT53_002333"
     CDS             2136..2840
                     /locus_tag="IXT53_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ4906124.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2845..4680
                     /gene="walK"
                     /locus_tag="IXT53_002334"
     CDS             2845..4680
                     /gene="walK"
                     /locus_tag="IXT53_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HAQ4906125.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4677..5993
                     /locus_tag="IXT53_002335"
     CDS             4677..5993
                     /locus_tag="IXT53_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906126.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            5994..6863
                     /locus_tag="IXT53_002336"
     CDS             5994..6863
                     /locus_tag="IXT53_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906127.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            6918..7727
                     /locus_tag="IXT53_002337"
     CDS             6918..7727
                     /locus_tag="IXT53_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ4906128.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(7830..8459)
                     /locus_tag="IXT53_002338"
     CDS             complement(7830..8459)
                     /locus_tag="IXT53_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase"
                     /protein_id="HAQ4906129.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8629..9921
                     /locus_tag="IXT53_002339"
     CDS             8629..9921
                     /locus_tag="IXT53_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ4906130.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(9997..10659)
                     /locus_tag="IXT53_002340"
     CDS             complement(9997..10659)
                     /locus_tag="IXT53_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HAQ4906131.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            10884..11168
                     /locus_tag="IXT53_002341"
     CDS             10884..11168
                     /locus_tag="IXT53_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HAQ4906132.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11219..12844
                     /gene="groL"
                     /locus_tag="IXT53_002342"
     CDS             11219..12844
                     /gene="groL"
                     /locus_tag="IXT53_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HAQ4906133.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4369 a         2265 c         2833 g         3679 t
ORIGIN      
        1 taaaaaaaga acagcgtaaa tgttttcgaa atgctttcgc accaacattt acctgttccg
       61 tgggtttggc acccaatggg ccgtgagggg ctcgaacccg cgacccgctg attaagagtc
      121 agctgctcta ccaactgagc taacggcccg tacaaaaagt acttaataat agtagcacta
      181 agaattttaa aatgcaagac tacatttaaa gaaaatcaga aaaatcttta cttccttata
      241 ggaaatcatt catagattat tcacatttgc tcgctattat ataagcatac caacaaacaa
      301 agttcttgca agagaacaaa ccctaacact tttttcattt tttactcctt tcccgccgtc
      361 tccccggcgg gtctcctgtt cttaacgata aaagacaaga aggcttagcc ttcttgtttt
      421 ttgttttcat ggaatcccgc taatcaaatc acctagttaa agcaactaga ttgaatgttt
      481 atcataagtc tgatatcatg tttatgaaat gaaaaaagga gcggttatga tggaaaaaat
      541 gcgtcagcaa ttaatggagt gggggaagga actagaaaca ttccgtctgc ctcactggga
      601 agaactccct gacttagaac tttatatgga ccaagtcaga acgttagttg atcgttatct
      661 ttcacctgtg atacaaggag aaaagcatcc attgcttacc tcttctatgg taaacaatta
      721 cgttaaacta ggtttgatac cagcaccagt taaaaaaaga tataacaaag aacacgtagc
      781 atttttactt gcaatcacta cgttgaaaca agtattgact attcctgaaa tcaaagaagg
      841 gatattgttc caaggaaaaa cagtaggaat tcgtgaagcc tataatctat tttgcgatga
      901 acaagaagca gctgtgtgga tggtcagcca attagcacaa ggtaaatctc atccgcaaaa
      961 attcgaccag gcaacccctg tagaatatat tgctgtaaga gcggcaactt tatcgtttgc
     1021 aatgaaactt cttgcggaaa aaacgattgt tctagaaacc gaatatttaa aagaggagaa
     1081 aacaaatgaa aaacaataaa atcgcaattt tagtagattc aggtacggat gtgccagaag
     1141 aattgataaa aaaatacgag atgtatatga tcccattgaa aattatttat aaagatcagg
     1201 tatatacaga taaaattgac attacctctg aagagatcta tcaacgtttg ccagaggaaa
     1261 tccctggtac atctttgccg gatggtgagt cgatcacaga aatctttgag caaatcaaaa
     1321 aagacggtta tgaaaaggta cttgcggtaa caatttccag tggattgagc gggacatata
     1381 atgtggttcg tttaattgca caacagcaag aagaattgga agcatatgta ctagatacga
     1441 aaaatattgg tattggtgca ggtttttctg cgatccaagc agcaaaatgg ctggaagaag
     1501 gagtagaatg ggatcaactg atcagtaatc tgaatgagtt agtcgaaaga acaaaagttt
     1561 tctttaatgt tgcgacattg gaatatcttc aaaaaggtgg acggatcggt ttagttgctt
     1621 ctattgtagg aaccgcttta aagcttaacc caatcatctc atgtaatgaa gaagggattt
     1681 attacacagt aggaaaagct aggggacgta aaaaaagttt ggataaaacg gttgcttacg
     1741 tcaaagaaaa aatcggtaat gcaaaggtct tcaacttagc tgttgcccat ggggatgcga
     1801 aagaagaagc aattgaaatg gaagcgagac ttaaaagaga gttcccacaa gcaaaccaaa
     1861 tctactttgg ccagatttct ccggcattag tagtccacac tggtccggga ttattgggtg
     1921 tcggtgtaca agttttagaa ggataaatca aaaaaaggaa acactgaggt gaaaatcagt
     1981 gttttccttt tttattgaag ttagaacaaa aaataataaa gtattttgca ctttcatttt
     2041 aattttctct tttgttttat aggcaaagat gaaacaaaag cagaaatacg gtataatgag
     2101 aaggtatgac aataatatat aagaggtggc tctttatgaa aaaagtttta gttgtcgatg
     2161 atgagaagcc gatttcggat atcgtcaaat tcaatttagc caaagaaggc tatgatgttt
     2221 atactgcata tgatggggaa gaagccttgg aaaaagttgc tgaggtagaa cctgatttga
     2281 ttttacttga tttgatgcta cctaaaatgg acggcttaga agttgctcgt gaggtacgca
     2341 aaacttatga tatgccgatc attatggtaa cagccaaaga ttctgaaatt gataaagtcc
     2401 taggcttaga gttaggcgcc gatgattacg tgaccaagcc attttccaac cgagaattag
     2461 ttgcacgtgt caaagcaaat cttcgtcgcg gtgccacggc tgcgaaggaa ccagaagagg
     2521 cagcacctgc ggaattgacg attggtgatt tgacgatcca tccagaagct tacatggtga
     2581 cgaaacgagg agaaacaatc gagttaacac atcgtgaatt cgaactactc ttttacttgg
     2641 cgaagcacct tggacaagtg atgacaagag aacatttatt acaaacggtt tggggttatg
     2701 attattttgg agatgtacga acagtggatg taactgtacg tcgtttgcgt gaaaagatcg
     2761 aagataatcc aagccatcca aattatttag tgacacgtcg tggtgtcggt tactatttgc
     2821 gtaacccaga acaggagtaa ttggatgaaa agaaaagtcc gcttttttcg atcagtcaat
     2881 tttaagatcg ctattacctt tattctgatt ttattgatct cgatcgaaat tattggtgcc
     2941 tactttatac gtggtttaga acgttccacg atcaatacat ttattaaaga tatgaatcaa
     3001 acggtggaat cattagcaac aacaatcagc ccggaattga atcgaaaaga taatgcagat
     3061 gatgaagagg taaatgcgaa tatcaaaaga tttattgaaa acagtgcgac tagcgatatc
     3121 atcgaaatac gggtcgttga cgaaaaaggg atcatccgag gaacaaccga tgtcaatgaa
     3181 caaagcgctg tgggaaagaa aaatgattat gttgatatca atgatttcac gactaaacgt
     3241 tatgttgctt tagataacga cgataaacgg gtgaatatca atgttcaacc gatcctgtcc
     3301 ccgaccggcg atgccgttat cggggctctt tacgtcaaaa gtaatatcga acaaaaatat
     3361 tcggaaatca ataacacagc agtcattttc tttactgcct cactaattgc ggcatttatt
     3421 tcaatgatcg tatctgtttt agttgctcgt tcgattacac agccgatcgg tgaaatgaga
     3481 gaacaagcca ttcgaatcgc tagaggagac tacagtcgga aagtcaaagt gcatggacaa
     3541 gatgaacttg gacagctggc tgataccttc aatcagttag ctgaacgtat tgaagaagct
     3601 caagatacaa tggaagctga acgcaatcgt ttggatagcg tgctatccca tatgacagac
     3661 ggcgtcattg cgacagatcg caggggtaaa gtcattacga tcaatgatat ggctgtttcc
     3721 ttgctagata tcaaaaacga ggaagcaatc ggacaatcga ttttgacact tttagatatc
     3781 gaagaagaat atacgttgcg gaaattatta gaagatccaa acgaaatggt acttgaccgt
     3841 tcaaccagta cattggaaag tgatcagatg atcttgcgag tagacttttc tatgatccgt
     3901 cgcgaatcag gttttatcag cggattagta gctgtcttgc atgatgtgac agaacaagaa
     3961 aaaacagaac gggaacgtcg tgaattcgtc tctaatgttt cccatgaact tcgtacaccg
     4021 ttgacaagta tgcgtagtta tatcgaagca ttgagcgaag gtgcgtggaa agacgaggaa
     4081 gtagcaccaa attttttgaa ggtaacgcta gatgaaacag accgaatgat tcgaatgatc
     4141 aatgacctct taaatctatc tcgcatggat acaggtaata cccagcttca attagaatat
     4201 gtcaatttca acgagttagt gaattttgtt cttgaccggt ttgatatgat ggtgggaaat
     4261 caggaaaaaa attataagat ccgtcgtgaa tttacgcaaa gagatctttg ggtcgaagta
     4321 gatacagata aaatcatcca agtagtcgat aatatcatga ataacgcgat caagtattca
     4381 ccagatgggg gaacgattac ttgtcgctta ttagaaacgc ataataacgt ggttttaagt
     4441 atcaccgatc aaggattggg gattcctaaa aaagatctga accgagtgtt cgagcggttc
     4501 tatcgagtag ataaagcacg tgcaagagca caaggtggga caggacttgg attagctatt
     4561 tctagagaag tgattaaggc gcatcgaggc gctatttggg cagagagcaa ggaaggaaaa
     4621 gggtcgactt tttatatctc gctgccatat gaaccttatg aggaggaatg gtgggaatga
     4681 gaatctctga aaaagtcgtt cgtataggac tcattttgat ggttgcgtta agcatatact
     4741 tctcttatgc aatctggctg agcccagcag gaaaaagttc aatcaacgta gatgagacaa
     4801 acactcaaat gatcgagtct caaagcaatc gaaaaacttc cgaagtcttt cttccgcttc
     4861 atgttacatg gcaccattcg ggaatgatcc aagaaaccaa cagtgaaaat ctcgtgtacc
     4921 gattgcaatt gatcatcgat aaagcaagat ttggaaagct ttctgaagta gtttcaggag
     4981 acaaatcaca gtttgaacag gtgaaaaatc tgaactctgg ttttgagttg gcctataatg
     5041 cgcctttttt gctaagagaa tacaaggaag catttgattt agatttagat gtgtcgaact
     5101 tggcaaaaga aagcgaaaat atgtatttta ctagaataca attcgatcaa ggaaataaaa
     5161 aaatccgatt tttgaattat tcgaaccatc tcgtatatga agcaaatatt tcgttgaatt
     5221 ggacagatat caataaagaa ttaacagcaa aagacacaaa atggacagaa atgacaacag
     5281 aaccatcgac gatcgacacg caatatgcta cgaaacattc gatcaaactg aaaaagtata
     5341 gttatatcct gtcacagcaa ccctatacac tttttagaaa tgcttttttc cagaaaccag
     5401 atagcgtgaa aaataatgat gaaagcaatg aattgatttt ttacggtggg aatgaaacaa
     5461 tgtctatcca ttcggaaacg caagtagttg attttcgtgg agagctgcca gagaaaaaag
     5521 aaaatgctag tgtattttct gaaagtttcc cctatattag caaattaggc agttctttag
     5581 ggaacctcag atattttgac cgcagtgaca atacgatcga ttatcggata ttcgtggaag
     5641 gcttcccggt ttttgggaca gacagcagag gacagattgg tgtggaagtt ggcggtgaaa
     5701 cgaatggttc tgtgcaagtg aatatccaaa ccagtttgaa tacgattcaa gtgccgatac
     5761 cttcagacga agaagtagaa cttgcaagta cgaatgaaat catccagcag ttaaaagcta
     5821 agggagcaga tagcaacaag attggttcag tgatcgttgg ctatacatgg caaaatgtca
     5881 aagaaacgaa tcgtgtcgtt gatctcttac ctgaatggta tgtgaaatat aaagataact
     5941 ggtattcagc taacgacctt ctggaaaaat tacctggatt ggaggcgaat taattggatt
     6001 tcaaaaaaat cgaatggata tttttcgtcg cttttttagg gttgaatgtg tttttattca
     6061 gcagttatca tgacgcggta taccaagaaa acaatgtcat tcgctctaat cagactgaat
     6121 caatccaaca acgtctggct agcgacgata tcacttacac ggggaagatt tcgtctgaga
     6181 acaagcaagg ctattatctt agcgcggagc agaccaatct tagcacttca ttagcgaatt
     6241 atcgcaaggc tggtaaaagc ggcttgctgg caagtggtgt gtttttagac ggtgatgttt
     6301 tgacgaaatc tttatccagt tcagatgctg aaaagaacgt gatcgatcca gataaaatta
     6361 gtagttcctt ggaagatttt ttatctaaca aagacaatat tttattcggt aatgattatg
     6421 tgtacacgaa aaacttttct catttagaag aagaaccact agaaatccaa gcaagccaag
     6481 agtatgaagg catcccattc aatgatgata cagcaaaaat cgttctttct ttagaaaaaa
     6541 acgataatga ttatagcatc acgaaataca tgcagacgca tttatctgat atcgagcagc
     6601 tgcgcgaaaa gacggaactg tatacggaag aggaagcaat caatacattg tacatcaata
     6661 acaaaatacc aagaggatca aaaattttat ggcgtcagct agcttattcc tgtattctga
     6721 aagtccggga aaaaaatgtg tatgttcctg tttggcatgt agcgatcgaa acaagcgata
     6781 aagtggtcca aattgagagt gtcaacgctt tcagcaatac gatcgtgaca aacaatactg
     6841 taccaaaagt ggaagatcaa taaaaaagcg agtataatga aaaaagttta caaagtaaga
     6901 agggaagtag gagtcaaatg gctgaagaaa aaccagcttt taaaatcagt atcctagcaa
     6961 gtggaagttc gggtaattca ctttatatcg aaagtgaaaa aaaacggctt ttggtcgatg
     7021 caggacttag cggtaaaaaa atcacttcct tgatggctca aattgatcgg acaccggacc
     7081 aattagatgg aatacttgtg acgcatgaac atcgagatca tatacatggc gtgggtgtct
     7141 tggctcgtaa gtatcatttg gatgtttttg ccaatcagca gacttgggaa gcgatggaac
     7201 cactgattgg aaatgtacca gtggaacaaa aacatctatt tgagatgggg aaagtcagaa
     7261 cattcggcga tatcgatatt gagagtttcg gcgtctccca tgacgcagcg gctccgcaat
     7321 tttatcgttt ccataaagaa aatcggtcat tcgtcatgtt gacggacacc ggatattgca
     7381 gtgattatat gcgaggtgtc attgaaaatg ccgatggtta tttgatggaa agtaatcatg
     7441 atcttgagat gttacgcatg ggaccttatc cgtggaattt aaaacagcgt attttaggag
     7501 atagaggaca tctttccaac gaagacggtg cacttgtcat gactgatgtg atcggtaatc
     7561 ggaccaaacg aatctatctt ggccatctta gcaaggaaaa caatatgaaa gaacttgctc
     7621 atttaacaat ggaaaatgtt ttgaaagaaa aagattttgg cgttggacat gattttgaaa
     7681 tctatgatac agatccagat gaagcaactg agctattttc gatttaaaca gttcaatcaa
     7741 taaaaatata aaaagcagaa gataaaatca atcagggttg gttttatctt ctatttttat
     7801 ttttttagca tattaaaaaa ataaccgtat tatcgactga ctacactgat gtgctgcttt
     7861 tcatgtttct catccagtat ctcatctact aatgcaatcg cgtaatctgc catgctgatc
     7921 gtactgtcgc cagcagcgtt tctttgtaaa taatcatcac ttaacaaata agtacctgta
     7981 cgtttgccat gtggatcaaa taaagaagct ggactgatat aagtccaatg tagattttta
     8041 gattgtttca attttaacaa tccttcgtac atattgaaag ctgttggata gaagggtgca
     8101 tttttaggta cttggtcaat catacgagtc gtcttttctg tatctaagaa taacgaagag
     8161 gcacccccaa cgacaattaa gcgtgtatcg gttccttcaa gcatctcaat caaatgggac
     8221 aaagaagttt gatgcatttc ttcttttcct ttgggagcgt tgaaagcatc aacgacaaca
     8281 tcaaaagatg caagatcagt agttgtcaac tcaaataaat ccttttgtat aaacggaaca
     8341 tctgatggca atttttctgg atgacgcacg attgccgtca cgtctaaatc tctggacaat
     8401 gcttcgtcta aaataaatga acctgcatgg ccagtagctc caatgagtgc aattttcata
     8461 aaaaacctcc ttttttattc ctgtccattg taagcagttg ctgaatattc gtcaaagaat
     8521 ccgatttttg aaaagctcag tgcgcattcc tttcatggtt cgtgatcatt gggtatagtt
     8581 aaattaagaa aactgattga taaaaataga agttgaagga gataagaaat gaaagagtgg
     8641 ttagatcgtg cctttttacc aatgataaca tctgttgtga atcttagacc cttaactgct
     8701 ttgcgtaatg gaatgactta cacgatccca ataattattg ttggctcgct gtttttctta
     8761 tttgctcatt ttcctgtgga ttacatcgct cagtggatag acaaaacagg attttctatg
     8821 tattttacac aagtatacca ggctacattt gatgtgatag ccttttggat cgtttttgcc
     8881 attgcatacg cttatgcaaa agaagaagac atcgatcgct taccagtcgg cataacagcc
     8941 ttgtctgctt atctactgct tattcagcca ctaaatactc agatagatac tgtctggaca
     9001 ggtgctgaag ggatactagg agcagttaca actgggctca tcatcggatg gggctatgca
     9061 cgtttagtaa gacttgcgaa agggtggaag caaagagaga agatacctga aaatgtttgg
     9121 cagtctttta cttcattgat tccaatcatt gttatagtca cttttacgct tatcggttca
     9181 gcggtttttc aacagttgtt cagaatgact gttgttcaag gagtatggac atatgtccag
     9241 cttcccattc aatcgttcat agacacgttg tttggtgtgc ttttattagg tttttttgtt
     9301 ccgtttttat ggttatttgg tcttcatgga tctgcgatgg tcaatgggtt ggttagtccc
     9361 gtcttgcaag cgaattcatt agcgaatgca gaaatcttag cctcaggaca agagttgacg
     9421 gtggcaaatg gcggacatat cgttactcag caatttctcg atcagttcat gacggttacg
     9481 ggtgctggat tgacgttagg ggctgtattt tttatgacgg tttttgcgaa atcgaagaaa
     9541 tacagagaat taggaaatct tgctctttta ccagcctttt tcaacatcaa tgaatcaatt
     9601 attttttcca caccgatcgt gatgaacccg atgatggctg ttccttttat tttcgcaccg
     9661 gtcgtttcag gattgattac ctattccgct ttatattttg gattcgttcc tttatttaca
     9721 gcagtccaag tcccttggac aacaccgccg atcctctcgg gtttcttagt aggcggagtg
     9781 cctgcagcga ttttacaagg tatcgtgttg tcaattagtt ttttcattta tttcccattt
     9841 ttgaaaaaaa tagattctgc aaactgcaaa aaggaaagac agacaaaaaa tgatggagca
     9901 gcagaaaaga tgaacgacta aaaaacaaaa atactcctgt cgctttagat tgggaaaatc
     9961 accaatctag gatgacagga gtatttatag tcatgtttat actagccctt tttggacagc
    10021 taattgaaca agaacaacta atgtgttcat tcctacgtgt gtgatcatcg atgtccagat
    10081 ccgaccagtt gctttgtact ctaggctgaa gaagaaacct aggaaaaagt agattagcag
    10141 atgaccatca ttatgagcaa acgcaaacag caaagagctg atcactgctc cgacccaaaa
    10201 atttgaatat cgactgatga tccccagtat actgcggcgg aatacgaact cttccatgat
    10261 gggtccagca acagttgtcg ccaaaataaa agcgggtgct tccatgatca tctggatgat
    10321 attttgtgta ttctcggaag gggttgcttt gccaaacaat acagattcta acatgacagc
    10381 gatgctttgc agaatcaatg cgacaaagat tccaaccaat ccataaataa caatctttgg
    10441 agcagaagct ttttttaccg ctgattcgat cgctaagggt tcacgttgct taaaataaaa
    10501 aatagctagg acaacggcgc ctagtatata catgactgca gttgctgtga tcaattcaga
    10561 cgtagatttt acaagtccta tcgaagctag gatcaagggt gagataaaaa ttagaccata
    10621 gcaaaaaatg ctgaggaagc tatatttttt taaagacatg taattcctcc ttttaaggga
    10681 taacaagcat gcaaggcaag cttatatcga ttgatttcaa ttatatcata atttcaataa
    10741 gcagctgtca gaataaaatt tcttttcttt gatttaaaag cttgcaaaaa agagggagaa
    10801 tgagtattat taaaaatgta ggttagcact cgaaggttaa gagtgctaac agattaaaaa
    10861 aagagttatc ggagggattt atcgtgttaa aaccattagg tgatcgcgtc attatcgaag
    10921 tcgcaaaaga agaagaaaaa actgtcggag gaatcgtttt agcttctgct gctaaagaga
    10981 aaccccaaac tgggacagtt gttgcagtcg gtgaaggccg tttgttagaa aacggtgaaa
    11041 aagttcctgc cgctgtaaaa gcaggcgacc aagttatgtt cgaaaaatac gccggtacag
    11101 aagtaaaata cgaaggaaaa gaatatctga tcgtcgcagg aaaagatatc atggcgattg
    11161 ttgagtaaat ttggaagata aaaaaataat caaaaataat caatgaggtg aatcaatcat
    11221 ggcaaaagaa ttgaaatttg cagaagacgc acgcgcagct atgttacgcg gggtagataa
    11281 attagcagat acagtgaaag taacattagg acctaaaggc cgaaatgtcg tactagaaaa
    11341 atcttacggc tctccattga tcacaaatga tggtgtgacg atcgcaaaag aaatcgaatt
    11401 agaagatcat tttgaaaaca tgggtgcaaa acttgtatca gaagtagcat caaaaacaaa
    11461 tgatattgca ggagacggta caacgacagc aactgttctg acacaagcaa tcgttcgcga
    11521 aggtttaaaa aacgtaactg caggtgctaa tcctctaggc attcgtcgcg ggatcgaatt
    11581 ggcaacaaaa gcagcagtcg aagaattgca taatatctca actgttgttg attcaaaaga
    11641 agccattgca caagtagcag ctgtttcttc gggttctgat aaagttggac atttgattgc
    11701 tgatgcaatg gaaaaagttg gaaacgacgg tgtcatcaca atcgaagaat caaaagggat
    11761 cgaaacagaa ctagatgttg tagaaggtat gcagtttgat cgtggttact tatcacaata
    11821 tatggtaaca gataacgaca aaatggaagc tgttctagaa aacccatata tcttgatcac
    11881 agacaaaaaa atctctaaca tccaagatat tctgccattg ttagaacaaa tcttgcaaca
    11941 atcacgtcca ttgttgatta ttgctgacga tgtagatggt gaagcattgc caactcttgt
    12001 attgaacaaa atccgtggaa cattcaacgt agtagctgta aaagctcctg gtttcggcga
    12061 tcgtcgtaaa gcaatgcttg aagatatcgc tattttgaca ggtggtacag taattactga
    12121 cgatctaggt ttagaattga aagatgtaac aatcgaaaac ttaggaaatg cttctaaagt
    12181 tgttgtagat aaagacaata caacgatcgt agaaggttct ggagaaaaag aagcaatcga
    12241 agctcgtgtg caattgatca aaaaccaaat tgctgaaaca acttctgatt ttgaccgcga
    12301 aaaattacaa gaacgcttag ctaaattagc aggtggtgta gctgttgtta aagttggtgc
    12361 agctactgaa acagaattga aagaattaaa attacgcatt gaagatgcat tgaatgctac
    12421 acgtgccgca gtagaagaag gaatggtctc aggtggtggt acagctctag tgaacgttat
    12481 cagcaaagtt tctgctgtag aagcagaagg agacgtggca acaggtatca agatcgttgt
    12541 tcgtgcttta gaagaaccaa ttcgtcaaat cgctgaaaac gctggttatg aaggatcagt
    12601 gattgttgat aaattgaaga acgtggaact aggcactggt ttcaatgctg ctactggaga
    12661 atgggtaaac atggttgaag caggtatcgt agacccaact aaagtaacac gttctgcctt
    12721 gcaaaatgcc gcttccgttt ctgccttgtt attaacgaca gaagcagtcg ttgcagataa
    12781 accagaacca gcagcgccag cagctcctgc aatggatcca tcaatgatgg gcggtatgat
    12841 gtaagaaaaa gcaacttgct tatctacaac tgatcattag tgaaagacag ataaaaagag
    12901 gctggaacag aagcgtttaa ctccaagaaa taagaagaaa ttcacggaaa ttgcttttca
    12961 aatttttgtg aatttcagct tatttccgaa ggagttgctt ctgcttccgc tgtttatacg
    13021 tgtttagagc gtgagacaaa agtgttcttt acttttgtcc cacgctcttt tttgtgtgta
    13081 attataatga aaatagttat ttttaatagt tataatattt tatctattaa aagtaaatat
    13141 ccattc
//
LOCUS       DACGLW010000084        12926 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.087, whole genome shotgun sequence.
ACCESSION   DACGLW010000084 DACGLW010000000
VERSION     DACGLW010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12926
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.087"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            complement(5..628)
                     /locus_tag="IXT53_002343"
     CDS             complement(5..628)
                     /locus_tag="IXT53_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906134.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(787..2184)
                     /locus_tag="IXT53_002344"
     CDS             complement(787..2184)
                     /locus_tag="IXT53_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ4906135.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(2216..3187)
                     /locus_tag="IXT53_002345"
     CDS             complement(2216..3187)
                     /locus_tag="IXT53_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ4906136.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            3561..3986
                     /locus_tag="IXT53_002346"
     CDS             3561..3986
                     /locus_tag="IXT53_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906137.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(4474..5175)
                     /locus_tag="IXT53_002347"
     CDS             complement(4474..5175)
                     /locus_tag="IXT53_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ4906138.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(5191..5631)
                     /locus_tag="IXT53_002348"
     CDS             complement(5191..5631)
                     /locus_tag="IXT53_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HAQ4906139.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(5631..6206)
                     /locus_tag="IXT53_002349"
     CDS             complement(5631..6206)
                     /locus_tag="IXT53_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HAQ4906140.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            complement(7054..7533)
                     /locus_tag="IXT53_002350"
                     /pseudo
     CDS             complement(7054..7533)
                     /locus_tag="IXT53_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015510350.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(7692..8393)
                     /locus_tag="IXT53_002351"
     CDS             complement(7692..8393)
                     /locus_tag="IXT53_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ4906141.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(8501..9910)
                     /locus_tag="IXT53_002352"
     CDS             complement(8501..9910)
                     /locus_tag="IXT53_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HAQ4906142.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            complement(9958..11823)
                     /locus_tag="IXT53_002353"
     CDS             complement(9958..11823)
                     /locus_tag="IXT53_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ4906143.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            complement(11944..12795)
                     /locus_tag="IXT53_002354"
     CDS             complement(11944..12795)
                     /locus_tag="IXT53_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ4906144.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
BASE COUNT         3689 a         2786 c         2109 g         4342 t
ORIGIN      
        1 tttttcattg tccataggat tgatattgtc cttgtgatga ctcattcgta ataattgagg
       61 cttgattttc ttcacttccc gttatcgttt gtccactgcc agaatctgaa ggacttgttc
      121 ctgtcgactc gtttttttga gaagagacag ttgttttctt gacttgttca tttgtctttt
      181 tccattccgc taaacgatcg gcgaccactt ttttgtctac tttttcagaa taagcggtga
      241 tcacttttcc atttttcaaa cgggctactt tgtcgccatc atgagtcaaa agcaaaacgg
      301 tcaaatgctt gtctttaaat tgatattgtc tgagagaaat cgcactaagt acgtcatcga
      361 tattaataaa ttgattttct tcttttcctt ttggcaaata aatgtccaat tcgccttttt
      421 cgtaattggc agttatttta tactccgccc accaataagt tgagaattct tttttgaaat
      481 tatctactga ttctttgctt gctacgattt tttctgtttc tttctgagca agatctgctg
      541 tcatttttcc tgaaaatgct gcggataaaa taagcagtaa gaaaaaaccg aataaccaaa
      601 agagaagaaa cggtcttttt ttcctcatgc tttcctccac tcctatttaa ttttgactat
      661 tctattatat acaaaaaatg aaatagaaag acaaaaaagt tgaaaaagat cgtaaataat
      721 tgaacttctt tttcacaaaa tggtaagaat ggagaatttc acgcatttgc tctcaactat
      781 tctttcttaa gcctcaaatc cgtttttatc tgatacgtct ttgaaccaat gacccgattt
      841 tttgattgtt ctttttccat cttgtgctaa gtctactgaa ataaagccat atcgattctt
      901 atatgcattg gtccacgacc agttatccat acatgtccac atatgatagc ctaaacaatt
      961 ggcaccttct tgaatggctt gatggacata tttcagatga gattggacaa attcgatccg
     1021 ataatcatcg tggatcatgc catcttcttt gataaatcgt gcttcgtttt ctacacccat
     1081 accgttttca gaaataaagc agtcgatatt tccataatga tctcgtacat tgatcaaaat
     1141 atcatagatt ccttgttcat agatctccca tccgcgataa ggattcattt tacgaccagg
     1201 catttcataa ttgtcgaaat aatcctcagg cattggaccg tttttagtat cgattggtgt
     1261 ttcttttgct tttacccggc gtggctggta ataattgata cctaaaagat ctactgtatt
     1321 ttctttgata accgtcaaat cctctgcatg gatcacaggc atgagatcaa gttcttttac
     1381 tatctcaatc aattcacttg gaaactcgcc ttttacagct ggatcaagga atgaacgatt
     1441 gaagaacgca tctgctattt gcgctgcttt aacatcttca gggtttgatt cgtctcttgg
     1501 atagcttggt gtcagattca agatgatacc aataacacca ccttgctcca tttcatggta
     1561 agatttgatg gctaacgctg aagccaacgc ttcatgatag ccaacttgga tcgctttttt
     1621 catatctgac tcttctggat aatgccatcg atataaataa cctgcttcga ctggaacgat
     1681 tggttcatta tgagtaaacc atttttttac tttcgaacca aacaattcaa aacaactttt
     1741 ggcatatttt tcataggctt caaccgtttc tcggttcaac catccgcctt tttcttgcag
     1801 atacatcggc atatcgaaat gatacagatt cacaaagggt tcgataccgt tttcaatcaa
     1861 ctcatcaaaa taacgatgat agaaacgaac ggcttcttcg ttgattgttc cagattcatc
     1921 aggtatcaaa cggctccatt ggatagatgt tcgaaatgaa ttatgcccag tctgtttcat
     1981 caactgtaca tcctcttgat acttcgtata aacttgcgag gttttttcag gtcctacttg
     2041 atcaaagaac ttttgcggtt cttttttgta ccaataatca aaaatgttct ctccttttcc
     2101 atcgcctgaa atccttcctt ctgtttgtgg gccacttgct gcagaacccc accaaaaatt
     2161 tgctggaaat tgatattttt tcatcaaagc acctcttttt cttttttagt tcgttttagt
     2221 tgattccatg tatgatattt tccatcacaa tcactttttc attttcttcc tcttcttttt
     2281 tgattcgttt taacagctgt tctgctgctc gttccccgat tttatctgtt ggtggcgtga
     2341 tcgtgatttt cggaaggata ctcagatcgt ttttatctaa ataatcatag tgactgaacg
     2401 caatatcctt cccgattctg acaccttctt gattgaaaca ttcgatggca ccaatcgaca
     2461 tattgtaatt gctgacaaaa atagcagttg cccctttttc taataatgct tttgccccgt
     2521 aatagccgct ttttctagaa tagtctcctg taaagacctc taattggatc gatggatcat
     2581 tttggaccgc ttctttggct cctgctaaac gttctcttgc tacgtagtct gtttgagtgg
     2641 cagcaataaa tccgattttt ttatgtcctt gttcaccatg atgagcgatc acttttgcta
     2701 cactttctcg atcattcact aatattgtat caatacctgc tggtccttct ggacaattca
     2761 atgcgataac tggaatactc atttttgtga gcatttcaaa tccagaccac tcttcttcgg
     2821 ataagaagac aatcagccca tcgatcacat gcttcatcag gtattcaatt ttttgttcaa
     2881 ttttttctgg ctgatcggca aacccgctta ataataagct atacccattt tcttcagcta
     2941 ctctttcgat ccctgagaca acttccgacc caaaacggct ggaaatcccg tttatcaaaa
     3001 gtccaataga gaagcttcga ttattcttca accctttagc aatgatgttt tctttatagt
     3061 ccaacgctga aatcgcttct gctatttttt tcatgttctt ttccttcagt tgctgaccat
     3121 tcaagtaacg ggaaactgtt cccactgaaa caccagccat cttagccaca tcacggatcg
     3181 atgccataca ttctcactcc tgccagttac atttgatttc tctttcttgt tgttcgatta
     3241 tgatgaccgc attctttgca tagtgttgct tgtcatcaat atccatataa ctaaagtctt
     3301 cagtagagca acttcctctc actattaatt atacttaaac aagtatttga accggttcat
     3361 ttttaatata gcattttttt aaaagattgc aacacttttt taaaacgctt tctgaaagtg
     3421 gcggattttt tgccttttta aaattttatt ctaaaattta ctctatttgt gggtgttttg
     3481 tgcgtttttc tcttccatcc gtttgaagtt ttacattcca atggtataat ttctttatcc
     3541 tatcaaatgg gggaagtatt ttgaagaaat taatcgtact atttattttg tctgcgggac
     3601 ttttgacatt gactggctgt gatttgtttg gcggctctga ccaatcctcg gacgaaacaa
     3661 ccgattcttc tgtcttaaaa gcggaaaaag cagataccac caacagtgta tatacgctaa
     3721 aagtacgtaa agatatggat ttatccgcgg gacagttgac atttacagat aaaaaaatgg
     3781 aatggacacg cacgtatcag acacaaggaa ctgattcttc tgaatcatct gaagaaaaaa
     3841 cagtgctgac agatatcaag atcactacca aagatgacga atatattatt tcaggaaaag
     3901 aaaataaaaa agaaacaaca gtcatattta caaaaatcgg taattaccgt ttgaaggatg
     3961 aggatggcaa catctacagt ctatagtgcc gtctgttgga tcttcttttg tttctcccct
     4021 tcttttctgc tatagtaaag aagcaagatg attgtaaagg agacgaaatg gatgatcgat
     4081 agtcctgatt attgtacatg taataacaac aaaaatatcc atacagaatt taatgaccaa
     4141 ggaagcttga gcatatgcga caactgtcac aaactagtca aagattcatt taaaacattt
     4201 gaatcttatc ctgttcaaaa ttatgtaaaa agtagaagaa gataaacaaa tccaagcaaa
     4261 actcactatg gctgattttt atgaacttca aactcagctt cttcctttga tcaagcttca
     4321 tcagtaaaac aaacagtgga tcatttcagt ttacaaatca aagagtaaag atttatttat
     4381 ttcaaatatt tgaatataaa ataaccaaac gatagaaagg cttattctac atcacgtagt
     4441 aatttttctt tcccgtcgtt tggtttttta tttttagcct aattcttgac cttttgcatg
     4501 ctggttatcc gtaaaatatt ttccagcagg ctttccttcc aattcagcta atttttcttg
     4561 gatctgacgg atcacctttt ttgcatcttc tggattttct acaaaatcga atttatcccc
     4621 atccacttgt attttaggac atacatcaaa atcttcatac cattgattat agcgggtgtt
     4681 caacgtttca taataggtat ataacgtctc atcgctttct agctgttcat agtcacgccc
     4741 acgttttttg atacgttcta gcatcgtgtc caaagaaact ttgatatgga ccatcaaatc
     4801 aggccgtttt tttggagcaa catcatctaa ctcgtttagc atcgtatcta ataagctatc
     4861 gtactgctgt acttcgatat ccgttacccg acctaaatca gcgtttagat ggaacaacag
     4921 gctatcttca tagatggatc ggtctagcac attgtcatcg ttcactaaag catttttcat
     4981 tgctaaaaat cgcttgttca aaaagaagat ttgcaataaa aaagcgtact gctcaggatt
     5041 tgaataaaag agcggcaata cttcgttatc ttcgacattt tcataaaatg gtttagaatc
     5101 aatctctttt gcaagcatat ccgtcaaact gctcttacct gcaccaatcg ttcctgaaat
     5161 caaaatcact gacatacaac ccttcctctt ctataagtac tcgcctacgt aagtttcaac
     5221 aggcaatgtt tcaaaatcat attcacgtac agcttggtct gattttaaat acgtatggag
     5281 actttgtgta atcatcaagt tcgtaggtac cgtctcttct tgtacaatga tcatcggttt
     5341 tttcaacata gttgccacac ctaattcgta agccgttcct ggatcgattg tttgatgctc
     5401 aaagtcaagg atagcaacaa caaattctgc atttgtcagt tcttccatat ctttttcgta
     5461 tacttcctgc gcccatccag cgctgaatag ttcataattg ctttcttggt gatgacgtgg
     5521 agagtagaaa cttgtgactg ttttgttttc ctccaacgct ttttcgatac gacttactcg
     5581 atcgatttgc tcctctgaaa aaaatggtcc tgctaaatat atttgactca ttattttgcc
     5641 tccttcaata ataactgatt tttaatagat actgctcgta attcattgac cagttccaat
     5701 ggattgttgg aaaagattac ttgatccaac cattttttat ccataaaatc ctcttttgca
     5761 aaagtttcta ataattgttg caagccgctg taataaccgc caatattcaa tattccgatt
     5821 ggcttgtcgg gcgtcagacc gattgctatc caagaaagaa gttgtgagaa ttcctccaat
     5881 gtacctagac ctccagggaa aacgaggaca gcatctccct tttcaaataa taataccttt
     5941 ctttcatcga tcgtttctgt aggaataaat gtagtaacat cttttccagg caattcagtt
     6001 tcaaaaagcc cattcggata gatacctgtg acttttccgt tattttccag tactgtttta
     6061 ctaatcactc ccataatccc ggagtatgaa ccaccgtaaa ccagttcgat ttcttcttgt
     6121 gccatgtatt ttcctaacgt ttgcgcaata aatttgtaac tttctttatt accaaaacgg
     6181 gaaccgcaaa aaacagtaag tttcataaaa aaacactccc taactcaaaa gtctatcttc
     6241 attctacacg tgttttttta aaataaaaga ctagattttg tagtttttta gtggtatata
     6301 ggatagacgc cactatatat agtatttaga aaaaaatcat ttttcagcca ttttatagat
     6361 aaaacaaaaa taaataacgt gttttcatcg atatgaaatg acttacttat agaagatact
     6421 gggatttcgt cctatatgcc aataacgtgt attattttaa acaacaaata cttgtttctt
     6481 ttattaacga aaaaatcatt ttgttctcgt gttatgtttc actgataaat gatgtttctc
     6541 taaataaaaa agccgacgaa ctaaacgatc aaaatttgat catttttgtt catcagcttc
     6601 tcctaaaaat ctttttgtta acgataaata gctggatttg taaaaatatc acttgaatcg
     6661 tcactgctgg aagaaaattc agtgataacc gcacctgttg cgcctgcctg gaactagtgt
     6721 ttgatattgg gctcgaacgt gtactgatct ctagcatgaa gaataatttc tttttttact
     6781 gtataataac tttctttcct ttttggaata gtacaggaaa gtttttttgt ttcctttcct
     6841 tcgacataag gtataccaga ccctttcgac aacgaaaggt tctcattttt ccttcatcgt
     6901 catttcaagg agggtgccga tgctctggac agtttcgatc gctcctaaac caaaatctgt
     6961 atattcgatt ctttctaact ctgattcttt cataccattc ctgcctcaag gtacatctat
     7021 tgtacttttt tcttgtaatc cattctgcct ctcctaacta attgtcaaat catcccaaga
     7081 tatgtcttct tcctcttttt cttcttggat gatttctgca ttcggatcta ctggtttttt
     7141 caataataaa atggttattc ctgtgacaaa agaaccagct aacaatgcac caatgaatgc
     7201 ccatggtctg gtcatcgtag cgactgcaaa cattcccgat gccggaatac ggctatctgc
     7261 gccccaaaac atgcttaatc cgccacctac tgtcccaccg actcccgttg ctatgacatt
     7321 gcgtgccaag tcgttcaata cgatagggaa acatccctct gtgatctgac agatccccat
     7381 aggaaaagcg gtcttcaatg tatcgatttc ttgacgattg agtatatttt tacgaatgat
     7441 tttccctaag aaataagcga tcgtcaagcc gaatggggtt accatcgaag ctaaaatcaa
     7501 tacagtgatt ggttcataga ttccttctga aaatcgtgcc acaccccctt atccagaata
     7561 aatggtaatc cagaaacagc cgtgtatctg cggtaataag cctaaccaaa cgaaaaatat
     7621 gagaagctat ttttgtttgg ctgttcttat tacttgatgc agggcagctt tttcacaacc
     7681 tcttttttta tttatgtttc ataatcaata tggtaaggat gcagctccgt atggtagacc
     7741 gcctccgaat attcaatcat ttccccaatt tgcgtagacg atttccgtat ccgtttgatg
     7801 ccgatctgtg cgtctgtttt cagaattcct tgttcttctt ctgttaaaaa tactgccgag
     7861 aaatcatcag aaaatgagtc aatagtatat ccttttttcg tcagcaaacg atacaaagat
     7921 tctttgccaa attcttctct gctgaattgc atcatttctt gtggaagata atgaacaaaa
     7981 taaatataag gctgattgtt caaataatat aagcgcttaa aacaaacagc ttgctcaccg
     8041 aaaaaggaaa acaaaggatc ttccttcttt atatggacca attgcaaatc cagcgtttct
     8101 ttccggactt catatcggga gttctccaag atttgcgtaa agcttgccgc tttggataat
     8161 cgattgtaag gacgattgct tagaaccgtc gtcccacgac cacttttctt tgcgacatat
     8221 tcatctgatg ccagcagttc gattgccttt cggacagtga ttttgctgac ttgaaacagt
     8281 tcttctaatt cactttctgt cggcaacatc gaaccgactg gatattttcc tgagaaaata
     8341 tcttctttaa ttttatctgc gacttccaag tatagtacac gtgtcctttt cataacgtca
     8401 ccctttgctt tactattact aaaagaatag cacataaaaa ataaaaaaag aacacaaatc
     8461 acatgtgaga agtgtgttct ttttcattca gctgctttaa ttacaagttt acgccatttg
     8521 aggcaattac ttctttatac caatcaaaag attttttctt tgatcgattc agtgtcccat
     8581 ttccttcatc gtctagatcc acatagataa agccgtaacg tttactcatt tctcctgttg
     8641 atgcactgac aagatcgata catccccaag gcgtatagcc catcaaatct acaccgtctg
     8701 ctactgcatt agccatcgct tcgatatgac gacgtaaata atcgatccga taatcatcaa
     8761 taatcgtgcc atcctcttcc atatgatcaa tggctccaag accgttttca acaatgaata
     8821 atggtttctg gtaacgattg tataattcat tcaatgcgat tctcagccct tctggatcga
     8881 tctgccagcc ccattcactt gcctcaagaa acggattttt tactcctccc agcaagttcc
     8941 cttctgaagt tgctgcttgt ggatcagtct cattgattgc cgtagacata taataactaa
     9001 atccgatgta gtccactgga tattctttta gcaactggaa gtctttttcc gtttgctcta
     9061 atttttccgg atcgactcca tattttttat gggtacgcgc tgtgtatgca gggtattctc
     9121 cacgtacttg gacatccgtg cagaaaaaat tgaattcttg gttttggatc attgttgctg
     9181 cctgattgac tggattggca tcgatgctgt aagttgttgc atagataatc atgcagccga
     9241 tttggatatc cgaacgcaat tcgtggccga tttttacggc tttgctactt gcaacgaatt
     9301 gattatgcca tgcttggaaa atattttggt actctcctgc tccattgctt tttactaatc
     9361 cttgacttaa agctggaaag tgaaaggccg aattgatttc attgaaagtc atccaatatt
     9421 tgactttact gctgtatctc tctaatactg tctgagcaaa tcgttcatag aagtcgatca
     9481 gttttcgatt tttccagcca ccgtattctt ttgccaaatg aagaggcatt tcgtaatgag
     9541 aaatcgtgat cacaggttca atatcatatt ttaagcactc atcaatcagc tgatcataaa
     9601 attctaatcc cgcttcgtta ggagtacctt cgtctccatt tggaaaaatt cgtgtccagg
     9661 caatcgaaaa acgataacat ttgaatccca tttccgcaaa caaggcaaga tcttctttga
     9721 aacgatcgta atgatcgatt cctcgatgat tcgggtaaat atatttttct tgatcaatcg
     9781 tccagtcaaa ttcttcactg ccgataatcg caaagcgctg cttacctcca ggcatagcat
     9841 ctgcaacgga tagtcctttt ccgtctcgat cataagcccc ttctaattga tttggcagca
     9901 gttgctccac cccataaaaa tttttctgaa aaacgtgatt gcatcctgat ttcctcctta
     9961 ttttacaatt gccagaacat cttcgtttgc aataatctct ttttggtcta attcaagtat
    10021 atcggcaaat gcatcagtat tcgtaatgac aactggagtg attacgctat agccagctgc
    10081 tctaattgct tcgatgtcaa aacgaatcaa taaatctcct gctttgacga gatccccttg
    10141 tttgactaag atctcaaagc cattcccatt taattctact gtatccatcc cgatatgaat
    10201 caaaatctcc gctccgtcag tcgttgtgat ccctacagca tgtccagttg gaaaaatagt
    10261 cgtgatctct ccgtctgcag gtgcaactag ttctcctact tctggatcaa tagcgatacc
    10321 ttttcccatc acgccagaag aaaaaacttg gtctggtact tcactcaatg gcagtacttt
    10381 acccgttaaa ggacttttta aggtatgccg tgctgaagta atcggttctc ctgcattcgc
    10441 atgtttgttt tctaattgat tgtcttgtgt ttgttcgtca aatcctaaaa tcaatgtacc
    10501 tacgaatgca agaacaaaag caattcctgt agctattact gctactgtca catttgactc
    10561 tacaccatcc accgtattaa tgaaagtcgg aatcgtcaat aagctgacca aactgctgga
    10621 gaaagctttt actccggaaa atccgataat agctccacca atacctccag aaatacaagc
    10681 ggcaatgaat ggtttcttca aaggtaatgt cacaccatac acagttggtt cagtgattcc
    10741 aaataacgaa gtcactgtag aagaaatccc taatgctcga agtttcgtat cttttgttcg
    10801 caatgctaca gctaaagcag ccccaccttg tgccaagatg gctggaagaa gcattggcat
    10861 caatacatca aacccatatt gttcgatatt caaaaacatg atcggtacga atccccaatg
    10921 catgccgaac atcacgaata cttgccataa acttcccatg attagacctg ctacgattgg
    10981 actaaatcca taaatcgagt tgaagagacc gcctaaagca ttaccggcaa ccgtcccgat
    11041 tggtcctaaa gcaagaaatg ttaatggaac catcactact aaaacgacaa gcggaactaa
    11101 aatcatttgc agaaattgcg gaatcacttt ttttacgaat ggctctaatt tactttgaac
    11161 ccatacggct agaatgattg gtatgacaga agaagtataa ccagatggtg aaagaatcac
    11221 tgggatccca aagaaactga tagcttctcc cgcaccagca actgctgtga tagctggata
    11281 cacaagtgcc atagcaattg ctactgctaa aaactggttg gctttgaact ttttagctgc
    11341 tgtaaaagca agcatgacag gtaagaaggt aaagacacca tctgctgcag caaacaatat
    11401 ttgataagta ccgctatcag ctgtcagcca gcccatcacc gttgccaatg ataagaatcc
    11461 tttcaatacc ccggcggctg ccatcgcccc tagaaaaggt gtaaatatac tagagataat
    11521 atcgatcaga cgattcaaaa tatttttgga ctcttctttt ccatcctccg aaactgcaag
    11581 accttcctca tccacgatcg attgataaac atctgctaca ttactgccaa tcactacttg
    11641 atactgaccg ccactttgta cgacttggat cacgtccggg tcttctttta acttttgcgt
    11701 atctgctaaa gattcatctt ttaatctgaa tcgcaaccgc gtagcacaat gtactaaact
    11761 tttgacattc ttttgtcctc ctaccgcttc ccatacacga cgaccaactg cttgattatc
    11821 cattgttttt tccttctttc ttttttattt aaaaaataaa aacccagatt gaaaaaaaca
    11881 gtcacaaaca ttcgtgaacc tattttttca atccaggttt tgcctgccat acagtaacaa
    11941 tccttatgat tggatgcttt ttttctcact tactactctt tgaatatgaa tcgttaaata
    12001 aatctgctct gcgatcccca tttcctgttt ttgcgtttgt cgaagatact cattgatccg
    12061 ttcgacacaa tgaaacgctt ttggatactt tccttgaact aatgcgtaga gaaattcatc
    12121 ttgctcgtca gatccttccc ctttgagata acgctgggca aaaaactgca gatgagtaat
    12181 gatacgctga taattcaaag attcttcatc gaacacgatg ccgaagaatt tactgatgat
    12241 actcagtata tctcttacca ttgtggtagc ggtcatcgtc acttgcatgt cttcgtttcc
    12301 ttgttggcta ttcacaaaat gaaaggcaat aaaaccggct tcatcctcag aaagttccac
    12361 acccgtcttt tgtttcacga acaaaagagc ctctttcgct gcttgatatt ctttcggata
    12421 aaattttttt gtttcaaata ataatggatt aggcagttgg atattttctt ttgctcgtgt
    12481 gatcgcataa tgaagatgat cggtcaatgt cagataaata ttaggcgaaa attcaacttt
    12541 tagtttatct tcagccagtt cgactacttg tttgacccat tcgatctcct ctgccggaat
    12601 atcagccatc agctgctgga attggttttt agcaaaagaa tctcttaaaa caaattcttt
    12661 ttggaccgct tctggcgaaa tctcgtcccc aatctttttt tgaaaagcta gtccttttcc
    12721 catacaaacg acttcttccc cgaatccatt tttcgaaatg acgacattgt tattcaaaat
    12781 tttatcaatt ttcattttgc ttcgccctct caagaaaaat aaaaaaacct agaattcgcc
    12841 tactgtttct tagctgaatt ctggttttgc ctgccgtaca gtaacaatcc atttcttatt
    12901 caaaatcatc ataccatcga attttt
//
LOCUS       DACGLW010000085        12670 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.088, whole genome shotgun sequence.
ACCESSION   DACGLW010000085 DACGLW010000000
VERSION     DACGLW010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12670
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.088"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            6..305
                     /locus_tag="IXT53_002355"
     CDS             6..305
                     /locus_tag="IXT53_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ4906145.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="IXT53_002356"
     CDS             378..1739
                     /locus_tag="IXT53_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HAQ4906146.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="IXT53_002357"
     CDS             1761..2021
                     /locus_tag="IXT53_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906147.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="IXT53_002358"
     CDS             2035..2787
                     /locus_tag="IXT53_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HAQ4906148.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="IXT53_002359"
     CDS             complement(2881..3486)
                     /locus_tag="IXT53_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HAQ4906149.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="IXT53_002360"
     CDS             complement(3539..4513)
                     /locus_tag="IXT53_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HAQ4906150.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5670)
                     /locus_tag="IXT53_002361"
     CDS             complement(4891..5670)
                     /locus_tag="IXT53_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ4906151.1"
                     /translation="MLLRVVGSIIFRYTLEEVNNVPKYEEIANILRERIRSKEYPLDS
                     FLPNQVDLVKEFGASRMTIKKAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEY
                     EGLSLQMKRRHRSLKSQIIHFDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVL
                     EHTFMPVHLVPGLKKEHLLASIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAE
                     NDPVLEVQQVVYLKDGQPIEYSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IXT53_002362"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="IXT53_002362"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HAQ4906152.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="IXT53_002363"
     CDS             7161..8558
                     /locus_tag="IXT53_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ4906153.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="IXT53_002364"
     CDS             8639..9079
                     /locus_tag="IXT53_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ4906154.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="IXT53_002365"
     CDS             9227..9553
                     /locus_tag="IXT53_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ4906155.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="IXT53_002366"
     CDS             9554..10303
                     /locus_tag="IXT53_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906156.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="IXT53_002367"
     CDS             complement(10383..10838)
                     /locus_tag="IXT53_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ4906157.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="IXT53_002368"
     CDS             complement(11265..11540)
                     /locus_tag="IXT53_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906158.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="IXT53_002369"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="IXT53_002369"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HAQ4906159.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT         4056 a         2174 c         2587 g         3853 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt cattccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaaattta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag
//
LOCUS       DACGLW010000086        11962 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.090, whole genome shotgun sequence.
ACCESSION   DACGLW010000086 DACGLW010000000
VERSION     DACGLW010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11962
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.090"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            656..856
                     /locus_tag="IXT53_002370"
     CDS             656..856
                     /locus_tag="IXT53_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ4906160.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
     gene            1424..1987
                     /locus_tag="IXT53_002371"
     CDS             1424..1987
                     /locus_tag="IXT53_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906161.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(2048..2413)
                     /locus_tag="IXT53_002372"
     CDS             complement(2048..2413)
                     /locus_tag="IXT53_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906162.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(2489..2734)
                     /locus_tag="IXT53_002373"
     CDS             complement(2489..2734)
                     /locus_tag="IXT53_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ4906163.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            complement(2804..3058)
                     /locus_tag="IXT53_002374"
     CDS             complement(2804..3058)
                     /locus_tag="IXT53_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906164.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            complement(3222..3545)
                     /locus_tag="IXT53_002375"
     CDS             complement(3222..3545)
                     /locus_tag="IXT53_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906165.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            3732..3805
                     /locus_tag="IXT53_002376"
     tRNA            3732..3805
                     /locus_tag="IXT53_002376"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3766..3768,aa:Ile,seq:cat)
     gene            complement(4190..4687)
                     /locus_tag="IXT53_002377"
     CDS             complement(4190..4687)
                     /locus_tag="IXT53_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HAQ4906166.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(4727..5536)
                     /locus_tag="IXT53_002378"
     CDS             complement(4727..5536)
                     /locus_tag="IXT53_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ4906167.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            5870..6727
                     /locus_tag="IXT53_002379"
     CDS             5870..6727
                     /locus_tag="IXT53_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAQ4906168.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHEKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            6720..7622
                     /locus_tag="IXT53_002380"
     CDS             6720..7622
                     /locus_tag="IXT53_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HAQ4906169.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            7655..8005
                     /locus_tag="IXT53_002381"
     CDS             7655..8005
                     /locus_tag="IXT53_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HAQ4906170.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            8039..8977
                     /locus_tag="IXT53_002382"
     CDS             8039..8977
                     /locus_tag="IXT53_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ4906171.1"
                     /translation="MRYYFVDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPD
                     DIFVGTEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLI
                     TCIDRKTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAAR
                     KTTENEELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQ
                     RQTEKMIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTG
                     GLPGKSSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            9055..9948
                     /locus_tag="IXT53_002383"
     CDS             9055..9948
                     /locus_tag="IXT53_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ4906172.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            9986..10549
                     /gene="amaP"
                     /locus_tag="IXT53_002384"
     CDS             9986..10549
                     /gene="amaP"
                     /locus_tag="IXT53_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HAQ4906173.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            10563..10751
                     /locus_tag="IXT53_002385"
     CDS             10563..10751
                     /locus_tag="IXT53_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HAQ4906174.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(10887..>11962)
                     /locus_tag="IXT53_002386"
     CDS             complement(10887..>11962)
                     /locus_tag="IXT53_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ4906175.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT         4082 a         2020 c         2243 g         3617 t
ORIGIN      
        1 ttatttgtag tgaacaattt agaacaaaca caaaacgcat ctactgttat ctaccagtag
       61 atgcgtttaa agagaagaac gagaatcgtc catatgaggg tttccactcc ttatgcagat
      121 ttttttaatt cttgcactgc ttgttcgatc gtaacacctg tcgctacatg attggtattt
      181 tctttgtcaa aaacgacaaa tagattcaaa tctgtatcca aggttactcg atattgtata
      241 tctttgttat agaatgtcat agccattctc ctttctttaa atacttgctg aaaggcaacg
      301 aacgcgttga taacgaaatt ccatttataa gtataccacc aattgaaagg ttttcatagt
      361 taagaaattt taaagtcaaa agttgcagat aaaagcaaat aaacaagaaa acaaaaagtg
      421 aatcaatcgc ggttattcaa ctttttcttc gattcgaaga caatagaaga caatgaattt
      481 tatatttcat tgcattatcc ttgtgttatt agaaaaagca tgatatatta aatgagtaat
      541 ctattttgaa acgtaatttg agcgatatat ctacatagat aaaactcctt ttgcctatca
      601 aatatttatt gcaacgaaaa aacgtacatt gatacgtatc aggaggaatt tatttatgaa
      661 taacggtaca gtaaaatggt tcaacgcaga caaaggtttt ggctttatca ctggtgaaga
      721 tggaaatgat gtatttgcac atttttcagc tatccaaggt gacggcttca aaactttaga
      781 agaaggccaa gcagtgtctt acgatgttga agatggtcaa cgtggccctc aagcaacaaa
      841 cattgttaaa gcttaataaa aattgacttt taaccgtctt ttcgaaagaa aagacggttt
      901 ttttgtacga aaaaattcat tggtcgttga atgggctgaa tagtagtgta taaaacaatt
      961 ttgtggagcg tgaagctttt atggtagagg aaaatacgaa taaagtgatg ttgataaaaa
     1021 gattcctatc ttgaatatga atcgagatgt cttgaaaatg aatgagtttg accaatcatt
     1081 atcctatcga aatttacagt ttcgttcgat tggtctgtca attaatgaaa aagtaaaaaa
     1141 gaatatgttc taggaagagg gcggcacatc taaattttta agtaacggac agttcaaata
     1201 tcaagcattg aaagaggcta tcctactgtt tgaaggaata cgaatatata aaaacgacaa
     1261 agaggaaaaa gtagttcatt aaatcccttc aagtgatgaa agaatgagat ctaataaaac
     1321 ctgatctttc atctgttttt ttaataacgt aataattttt ttattttttg tgctttcgcc
     1381 gttattgtag agtgatttag cattcttatt tttaaaaata actgtgttat atattaaaaa
     1441 aggcataata gttataagct tttttaaaga atatttagaa attattaatt ttcgtctgaa
     1501 aacaaaaaaa gaaagcgtta ttattttagt ctattttcag ggggggaacc ttatgataaa
     1561 aaaactattt ttactatcaa tattaacgct agcaatggca ggttgttctg actctggctt
     1621 agcaactgaa ctgatcacaa aatcaactga agccacgaca gaggagttaa aatgtggaaa
     1681 cgatcacagt gactacagag gcttaaatcg acgatgcatg ggaatattag gcagaagcaa
     1741 ttgtaagcat cgatgtagta atcgaagaag gtgtagaaga agcaacagaa aaatcaaaag
     1801 aaagatcaaa agaagcaatc tgtgctgtgc atcgtataca gacgtcgaag atgcggtact
     1861 tttaaaccac gtcaggagat gttcatttat gtctagggaa gttggcgatg aaccaaaact
     1921 agagtacgct tttagttttg cacgtgagga tgaagataac cctaaaactt acgacatttt
     1981 agcttgatct aagatggtta aagcttttta atgaagtaag aggagataga aggcttttct
     2041 agttatatta ggtaagataa attccttttt gctccaaatt tgctaaacat tctgctagat
     2101 aaacttcatc gtgttccgga taaatcggca cgttgcgctc tacagtaggt aaatcaaaaa
     2161 atgcttcgca tgatttgcca cgatacatag gatttttcca ctctggtgct gggctgtaac
     2221 cacgtttttc catttcatcc atgacaagct gatgatattg gaataatcta taaggagaat
     2281 agtgaaaaac ataatccaca gtagcatgtt tctttcccca gccatttcct cttaatgcac
     2341 agcattcccg atgttgtcct aaaagctgtt gacgaggaag ctttgaaatc aatttttcat
     2401 gccataatcg catagaaatg tctcctttat atatagattg ttaaccttag acgttaaatg
     2461 aaaaaaccag caccaaggct ggaaaaaatc acttcagttt gcttgctgtt tctgagcgac
     2521 caattaggta atcaacggaa atatcgtaaa agtcagcgat atagattaat tcaatgatgc
     2581 ttggaatttc ttctccttct tcgtacttac gatatttgaa taaaggaata cttaacgcgc
     2641 tagaaacagc tacttgagat aaagatcggc cagttcttaa gaaattcaga cgacaacgta
     2701 aagtatccag taaaatagct tcttcgatac tcatttaatc acctttcatt atagtagaat
     2761 agttataatg actatagaaa gctacacatg caatcaatag tactcaatgt tttttattta
     2821 aacgtatcaa ataaagaatg acgatcaata aagacaaaac cagtcctgat aattcttcta
     2881 taaaaacact atcgggttca aagtagttgt aattaaaaac tttccacata aagtagatag
     2941 ccggaagagt caaaatagct aaataaaacc gttctttgtt ccaattcata agataaacaa
     3001 tacttgcgac cagtgcgacc gcaggcaccc aaaacaaaac tctcaccaac gtaatcatgt
     3061 aacggactcc tcaacgattc atataataaa agataacatg ttattgcata catcacaaat
     3121 gaaatgctag gaaaataaaa gaagaataga tcaatgaaaa atgaaaaagc agaacagttt
     3181 tctgctgata agcaggaact gttccgctga atgtgtctta tctatcgaga aaaaatcaat
     3241 ggattgatat ctaagtaatt caatacatat aaaaagagga cgatcgcatt cactaaaaag
     3301 gaaagaatga tgatcccata gcgaatggaa acatatttaa caaatacttt tgtttgaata
     3361 cgggcatatc tttttccttt atagctgagc atcatgacaa gaagcaaaag ggtaatcaca
     3421 ccttgaaaaa tcattccaat aaacagaatt cctgaggttt gataatttaa ttggagaaaa
     3481 taagctgctg ccgcaactaa atcgaataaa atcgcaataa atggcaaaat aatcactcct
     3541 aacattgaga ataaataaaa gaataacatt ttttctaaaa taagcaaata aaaattaaag
     3601 taacaaaata gttatgacac aagtaaaaga aataagaata acaatattat tttacttata
     3661 ttttgaaact tgtgagagtt tgtcattcgt gctatagttg aaaagtaaca aaaataaatt
     3721 ttatttatga tgtaccttta gctcagttgg ttagagcaga cggctcataa ccgtccggtc
     3781 gtaggttcga gtcctacaag gtacataaga ggttgtgaaa aagctgccct gcatcaagta
     3841 ataagaacag ccaagtaaaa acagcttctt atgtttttac ttggctgttc ttattactgc
     3901 agatgcaagc ttttgaatac cgtttaagga agaggttgtg aaatcagcgt tttgacctga
     3961 gtattagctt aacaaacgga aaaatagttc ctcatatttt gccgtttgtt aagctaatac
     4021 cgagggtcag ctgattgaac accatttaag aaagaggtcg tgagcctgcc gtttcactct
     4081 gtgtcataaa aaagccaatc tactttgata gaaaaagcac gtaggactaa aacagttctg
     4141 cgtgcttttt ttattgtgtg ttaaatatcg taaatgaaga tagatatttt tatgattcga
     4201 cgatagagac gttgaaatct ttcaacagct ctaaagcgga ttctccgtgt tttctactgg
     4261 agactaacat tggatcaaca aaaaagtctg cttcaggaaa agcactctgc aggcctatca
     4321 gattagagag tacacacaag tttgtttcaa tcccgactac ttcgatctct ttttgttctt
     4381 ctttgctttc aaataaagtt tcctggattt cagctaaagt ttcaggtgga atcgtaaagt
     4441 aatacttttt aatcactcgt tcattttctt gtggagaaag aagagggatc aattgcaaat
     4501 cttctcgttc ttcttctttg tccttcagtt caactggtat atcccgtgtg aagagaatat
     4561 attcattatt ttggcgtgct ttagctaatc tttgagcaat acgttctgcc aattgatctg
     4621 actctggaat ataaaattcg ctgtttggat caagaatatg attttgcata tcaataataa
     4681 ctaacatttt ctttcctcct acattcatat cgtcatttta ttctttttaa tagaagtttt
     4741 ttaaaatata agcaacacca gcttcttcat tcgtcagtgt tacttggttt gcaatttctt
     4801 ttacttgtcg ctgtgcattc cccatcgcaa tactcaaccc gctgtaattc aacatagcaa
     4861 tgtcgttttc ttgatcgcca aaagccatga tgtcactcga ttcgattcca aggtaatccg
     4921 ccagaaaaac aagtgaatga gctttagtga tacctgtagg cgtgatttct aagaaatgcg
     4981 gatcagagaa tactgattgg ataccatctg cttgcagatc tctttgtagc ttctgtagtt
     5041 tttcttcact cttatctgtt aatgctaatt taagatagct aagagaagga tcttccaaca
     5101 tatttaaaat ttgatcaaaa gaatggttct gtaaattgaa atgttcataa taagggtcat
     5161 ctttgactag atttgattca tcgaatgtca gctgatcatc ttgggtttct cggctagcaa
     5221 tgatggatat tttcgcttct tgagcataga agaaggcttt tctcagtgta tcgctatcta
     5281 tttttgaacg tctgagaact ttcgccattt ttccatcctt catttcagca atcagtgaac
     5341 cattggaaga aatcaaatag ccaccgattc ctaattcgga aaaaatatct ttagcagaaa
     5401 tgctgttcct tccggtagcg acggttacga ttccgccatt ttcatcaaat ttccgaagtg
     5461 cttggatatt ttcttctggt atctttccat ttcgatcaag caaggtaccg tctaaatcaa
     5521 tcgctgctag tttcatttta tcttcctact ttctttttac atatctattt tcctgttacc
     5581 ttaagattaa atgagaattt aaaaaaatga aactaacttg ctttgtcatt atgaattttt
     5641 tcaaaaaaca ttcttaagaa aaaggtggct atatatagta gtttttggtc aatacctttg
     5701 aataaaaagc catgaaaaac gaatagttag cgcttaccga tgcgttttgt gataaaaaaa
     5761 ttttaaaata ttcaatgaaa acaaagcata tattttgctt taaaaccaaa tgaggaatat
     5821 tctagatgta gaacaggtta gaaaaagatt agaaaaagga gtgattttga tggagaagag
     5881 atacggcgta gttcctgtgg taactgcttc agacgaaaat tatgctcctt acttgagcgt
     5941 gatgatcgca actgcattag aaaattgcaa taaaacaaga cgcataaaat tttatgtgat
     6001 tgacgatggt ctatcagaat atagtaaaga aggattggaa gaaacggtaa ataaatattc
     6061 aagtaatgca agtatccaat tcctgacagt ggaaaaagat atctacgaag acttcctagt
     6121 cagtgaccat atcacaacta ccgcttactt aagaatttct ttaccaaact tattagctaa
     6181 agaggattat aagaaagtcc tttacttaga ttcagatgtg ttagtactag acgatatcgt
     6241 aaaactatat gatgagccgt taaatggaaa aacaatcggc gctattatcg atccaggcca
     6301 agtgaaagct ttggaaagat taggaatcga ttccgatgac ttgtacttca attcaggagt
     6361 gatggtgatt gacattgatc aatggaataa aaaagaaatt acagaaaaaa ctatccatta
     6421 cctcagtgaa aatggcgatc gaatcatcta tcacgaccaa gatgctttga atgctgtact
     6481 ttatgaagat tgggaacaac ttcatccaaa atggaacatg cagacttcgc tgatttttga
     6541 acgtcatcca gcaccaaatg aaaaatatga acgtcagtat aaagaaggaa atgaaaaacc
     6601 ttcgatcgtt cactttacag gacatgaaaa accttggaat acgttgaaag atcatccgta
     6661 taccaattta tacttaaaaa aattagcaca cagtactcta acgaaagtag gcgagatcaa
     6721 tgaataaaaa agaaatagca gtagtcgcaa gttgtaacac aaaattcgta ccacatttag
     6781 ctgcattatt tgtctcagta ttagataatt gcaacccgtc taaatttgtt cggttctatg
     6841 tgattgacga tgatatcgat tttgaaagta aacagctttt acgcttctca gtgaaaaacg
     6901 cacgaatgaa ctcagatgtg gaattcttga aaatcaataa agaattcttt acaaatgtgg
     6961 taatcagcga tcgtattcca gaaacagctt attatcgtat tgcgattccg gaattgttcc
     7021 gcggcacaga agttgaacgt atcttatata tggattgcga catgatcgct ttacaagata
     7081 tctctaaatt atggcgtctt gacttcggtg attcaatcgt agcagctgtt gaagacgctg
     7141 ggttccatca acgactggaa aaaatgaaaa tttcagctaa atcaatgcgt tatttcaact
     7201 caggtttgat gctgatcaat gtgaaaaaat ggttagatga aaatattacg caaaaagtat
     7261 tagatttcat cgaacataat ccagaaaaat tacgattcca tgaccaagat gcattaaatg
     7321 ctatcttaca tgaccgttgg ttaccacttc atccgcgttg gaatgcacaa ggctatatca
     7381 tggcaaaagc aaaaaaacat ccaacagcag caggtgaacg tgaatacgaa gaaactcgca
     7441 acaatcctta cattatccat ttttcaggac atgtgaaacc gtggagcaag gattttgaag
     7501 ggccaacgaa gaaatattac gaaaaatatg cagggatgac ggctttccgt tgtgtagcta
     7561 aattccctaa atatccaaaa tatgcaaaag ttcaaccaaa aacagatgta gttgttaaat
     7621 aatagaagac aacaactgaa gagggggagt aggaatggaa attacaatgt ctaaaaatgc
     7681 agtagagacg ttaattgaaa aggttgggga aaatacaaaa atcgcactcg cgcttatcaa
     7741 tgattccgat ccatttctaa gggacaaagg tgcttttgca aaaggcagct tttttcaaat
     7801 cattcctttt gtttcggaat ttggagaata tgcgactaaa atcgagcatc cattattgga
     7861 catctataca tctaaattag agcaaaatta tttcggtaaa cgattgaaca tggacttcaa
     7921 caaacagctt gacagctttt cattagaaaa tgaaatcgca gtactcgatt ataatatcaa
     7981 attaaagaac tgcttcttca gctaatagtt tcgagaaagt ccaaaaagga ggtatacatt
     8041 gagatactac tttgtggatg aagaaaagaa tgtaatagaa acagatgaaa aaaaatataa
     8101 ttggctcgta cttgatagtg caaagcccga agagattgag caagcaatcg aagaattcga
     8161 gcttcctgat gatattttcg taggaaccga atatccggaa gaagtttctc ggatggagcg
     8221 cctttatgag acgaaactag agcatccatt ttctttggta gtgattaatc tagatcagtc
     8281 agataatcgt atcgaaaaaa aattaacgcc aatatctttt gtactttccg ataatctctt
     8341 gattacttgt atcgatagaa aaaccgaatt tgtggatcgg ctgctgacaa agcatggaaa
     8401 tcatttaaac tcgtttgaaa agatcatcac gtatacatta ttagatattt acacccatta
     8461 cgtgaaagaa ctaagagaga tgaaaaagcg aattgacgct ttggataaag cagctcgtaa
     8521 aacaacggaa aatgaagaac tgtataaaca ggcagatctt gaaagagaca tcgtatatat
     8581 cgatcatacc ttaagagatc aacgggaaac aatggatcaa ttatggaatt ctgaaacttt
     8641 catcaaacgt ttagacgatg aacagctact ctatgatgtc catttgagac agcgacaaac
     8701 agaaaaaatg atcgagatct atcgagattt attagagagt attggtgatc tattcaacgg
     8761 gatgatggac aataatctaa atcatatcat gaaatatcta gattcagctg cattaatcat
     8821 ttccgttccg gcgttgttcg cagggatttg gggaatgaat acaggaggat taccgggtaa
     8881 aagttctgct ctaggcttta tactcgtagt ctttggctct ttgatagcag cagtagtttt
     8941 aggttaccat ttataccgga aagattatac aaattaaaga atctatttaa aacaggtatc
     9001 aaacaaagaa atttgttttg gcaatacaat ttaaaaacta ggaggaatac agcaatgaca
     9061 gaaccaaaat taatcgaccc gcgaaacttg taccatacgg agaaatttcc taaacaagat
     9121 caggatacac cagctttaca gcagaatatg attcctgttc ctgattgtgg agaagaatcc
     9181 tatgaaggca ataatcaact agaaaaccgc cgtgtactaa tcactggtgg agattcaggg
     9241 atcggtcgtg cagcagcgat tgcatttgct agagaagggg cagatatcgc tcttcaattc
     9301 ttcccaggag aagaaaaaga tgcagaggaa gtagcaaaat acgtgaaaga tgcaggacgt
     9361 aagattgtct tactaccttt tgatcttcgt gacgaacaag cacctaaaga aatcatagat
     9421 aaagccgtga gtgaacttgg cgggctagat acgatggtac taaacgcagg acagcaaatt
     9481 tcttgtcagt ctattgctga gttaccactt gagcaagtca aagacacatt tatgataaac
     9541 atcatcgcta tgtttgcttt agtgaaagaa gctgttccgc atatcccagc aggaggagca
     9601 atcgtaacaa caacttctgt tcaagcattc aatccaagcg aacatttatt agattacgca
     9661 gcaacaaaag cttcaatcgc taactttacg atcggtttag caaaacagct tgcacctaaa
     9721 ggaatccgtg taaatggtgt agcacctggt ccaatctgga caccattaca actagacgaa
     9781 ggacaacctt ctgattcgat ccctgaattt ggtcagcaaa gtttactaga acgtgcaggt
     9841 cagccaggag aattagcacc agtttatgtt ttcttagcat cgaataaagc aagttatgtt
     9901 acagcccaaa tttacggagt aactggtgga gaagcgatca acttataaga agttgatcgt
     9961 ttcaccccta tgaggaggtg cgaaaatgaa taaaggtgta aaaacgatcg gagtgatcgg
    10021 aacattactt ttgctatctg tgcttttgtt tgcgatacta ttaagtgcaa cctatccatt
    10081 gccttttcag ctagagaagt ttcgattctt ttccatcact aattcatacg tgcaacaata
    10141 tgtattttgg cttgccgttg catttgctat tttgacaatt atcacgttac ttgttttcct
    10201 gttttatcca aaaacaatcg gaaactttct actgaaacat ggtgatgggg agttgacgtt
    10261 ggataaaaaa gcaattgaag gaatggttcg ttctcacttg catgaagatg aattcatcca
    10321 ttcaccaaaa gtaaatgtca aagcaacgaa aaacaagatc gccgtcaatg taaaagggga
    10381 gttgaaacga acttcagcac tcattggtaa aacaggaaca ttgatgaaag aaatcgaaaa
    10441 tgaagtacaa cacatattgg gaacaaaaga gccagtgaaa gtatcggtga aatacacagg
    10501 ctttgaagaa ccagctaaac gaagcaatga ccgcaaacga gtagaatagg gggggaccaa
    10561 aaatgaaaga actattttcg acatacaagc tccctatcat ctttggggta cttggattga
    10621 tattagccgt tttatggatc accgtgggct ttttcaagac cttattgatc ttgatcatgg
    10681 cagcgatcgg cgtcgctatc ggtttttatc tgaaacaaac gaaaatctta gatcactatt
    10741 ttaaatctta gtctaatggt gataagtcaa gatgaaattt aagattttct ttattgagta
    10801 atatgttttg aagcgcacga aactagaccc gatcaaaatc aaattttttt cgggttgatt
    10861 caaggatttg cgtcaataac ggattgttat ttctctatca cactccttgg tggcgtatag
    10921 agttggtgtt tacgtagtag cgtatccacc agacgcgtaa attttcttgc ggttaggacg
    10981 agtgcacgtt tgtgttgatg ctttggaacc tccttatatt tactttgata aaaagctttg
    11041 tattcaggaa gataattttt tacagagttg gcagcttcaa ctaagtagta tctaaaatat
    11101 cgatttcctt gttttgtcag aggtgtattt tgcgactgat agtttccaga ttggttcact
    11161 ttccaactta atccagcgta ttttgccaat tttgtctgat cttcaaatct ttcgatttga
    11221 ccaatttcag cgattaaacc ggcagcatag actttaccaa cgcctggaat actggttaag
    11281 cattgatatt ctggaatgac aacaattact tgttcgatgg ctttatctaa ttctttgata
    11341 tttttttcaa gtgctcgtat ttctcgtacg agaacactta aaacaacatt aattgattct
    11401 tgtgcaagag cacctaggcg gtaactcatg gtaattgcac gttgaattgc tttagcgatt
    11461 ttttctggtt gcttgaaacg gccttttcct ttttcttgaa gtaaatcaca gaaagcttct
    11521 aaaggaagct cagcaagttc atccaatgtg aaatcttccg tcatgagtga aatgagggtt
    11581 gcactaaaaa gactcgtcga ttcgtctcgc atttctcgag caagcgtatt acatttgtac
    11641 gttagatttt caagaaaatg ttgctttgta cgaatcaatt gcttaataag ttgatagcga
    11701 gtacgtgtta aacgctgtaa ggccatgtat ttctcttctt tgatagggga agttgtgaat
    11761 cgttgaatac gcaagaaatc agcgattctt aatgcatcgt ttcgatcagt tttattctca
    11821 tcaaacatgc gactaaattg tttcacccga aaaggatttt caatcgttac aagtgtgttg
    11881 agcttcttta gtttctcatc ttcatgaaaa aacatggaag gatggaagct gtacatggac
    11941 gttgactcca tgccaatcac aa
//
LOCUS       DACGLW010000087        11931 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.091, whole genome shotgun sequence.
ACCESSION   DACGLW010000087 DACGLW010000000
VERSION     DACGLW010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.091"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            51..260
                     /locus_tag="IXT53_002387"
     CDS             51..260
                     /locus_tag="IXT53_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906176.1"
                     /translation="MICYISFLILGLPQAVFPEMFSGDWGIVSYSILTLIAMVLGTSF
                     YNAFVSKHVEEYEVLMYLKEKKTSN"
     gene            467..667
                     /locus_tag="IXT53_002388"
                     /pseudo
     CDS             467..667
                     /locus_tag="IXT53_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1379..1984)
                     /locus_tag="IXT53_002389"
     CDS             complement(1379..1984)
                     /locus_tag="IXT53_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ4906177.1"
                     /translation="MAKIGYIRVSSQDQNLDRQLAMMEDLNIDKLFQEKASGKDIQRP
                     EFQKLLNYIREGDCVIVASLDRLGRDYEDIKNTVTFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAKKAGRYKGRPIEYSENSTDPQ
                     KRLVFKTVVDMLNQGVPVAEIAKENGLSRPTIYKIKKANDL"
     gene            2166..2501
                     /locus_tag="IXT53_002390"
                     /pseudo
     CDS             2166..2501
                     /locus_tag="IXT53_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2666..2893
                     /locus_tag="IXT53_002391"
                     /pseudo
     CDS             2666..2893
                     /locus_tag="IXT53_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613886.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            3019..3990
                     /locus_tag="IXT53_002392"
     CDS             3019..3990
                     /locus_tag="IXT53_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ4906178.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICEENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            4130..5443
                     /locus_tag="IXT53_002393"
     CDS             4130..5443
                     /locus_tag="IXT53_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HAQ4906179.1"
                     /translation="MKKRMLAIAAILALTAGGLAGCGSKEKSSGSSDPGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFIWSNGGDVVNADGTKAEDYFNGKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMMLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQLSGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            5486..6352
                     /locus_tag="IXT53_002394"
     CDS             5486..6352
                     /locus_tag="IXT53_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HAQ4906180.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLTPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQIGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            6349..7197
                     /locus_tag="IXT53_002395"
     CDS             6349..7197
                     /locus_tag="IXT53_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006639650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HAQ4906181.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            7211..8683
                     /locus_tag="IXT53_002396"
     CDS             7211..8683
                     /locus_tag="IXT53_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HAQ4906182.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWMNDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMIAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQERIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPDHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            8700..9572
                     /locus_tag="IXT53_002397"
     CDS             8700..9572
                     /locus_tag="IXT53_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ4906183.1"
                     /translation="MIYGSIEAGGTKFVCAISDEEMTIKERVSFPTTTPEETIPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKVAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
     gene            complement(9808..11190)
                     /locus_tag="IXT53_002398"
                     /pseudo
     CDS             complement(9808..11190)
                     /locus_tag="IXT53_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            11652..>11931
                     /locus_tag="IXT53_002399"
     CDS             11652..>11931
                     /locus_tag="IXT53_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906184.1"
                     /translation="MDSFQRLSLVNQFELLAKFEDQNRSYYERKIEILREGYEYHYDD
                     EIWSDLSEPFPKEDSRFVLDVLNMYRDINFSKSKLNLDENQITNSYYTH"
BASE COUNT         3701 a         2037 c         2401 g         3792 t
ORIGIN      
        1 atttaaaaag aaaagtgaca gataggaata aaaacttatt aaatgtagtt atgatctgtt
       61 atatttcatt tctaatacta ggtcttccac aagcagtgtt tcctgaaatg ttttctggag
      121 actggggtat tgtcagctat tctatattaa cattgattgc tatggtttta ggtacttcat
      181 tttataatgc ttttgtaagt aagcatgttg aggaatatga agtattaatg tacttaaaag
      241 agaaaaaaac aagtaattaa tatgagaaca ccacgcatag tttttatttt gagatattct
      301 agaatgaaac acattaagaa cgaaagcaaa gcaaaagacg ccagaacttc tactaccttc
      361 taagtttttt catatttgtt tgttatgcta tacatagatc tgcataatta gacttgcgct
      421 gcccgttatt gaagtttcag gcagcgctct ttttggggga tcgataatgg aagaagaaaa
      481 aatatttgag aaacgctggc agctagcatc aagtgaacaa agagcaagat acaataattt
      541 gatcacctcc taccccactg ttgactagac atataaggaa aagaaatact tactttggct
      601 gtgccagtta gatatagata cctttgaaac atttgaagtg atcctagata aaattaaaca
      661 gaattgatca gaaacaagcg aagccttctc tcggttcgct tgttttgcta tttattagtt
      721 ggctcagggc aactctaagc tcgtttgaaa gaaaaccgat ggataatccc cttgtttgta
      781 ccactttaga atacatggat gtacgtattg ccattgactc ctattagtta ctgaaaaaaa
      841 atttaaacaa gttgagtatt gcagataaat agctccagtg aggtgaataa ctgctccact
      901 caaggcaata gatagctcca tccagggcaa caaactgctc cactttcata tttaatagat
      961 gaataacctt ctgtataata gaaaggcata cctttcggta tgacaactta tacagaatgt
     1021 tattttattg gttcactatc gaaaaatttt ggagctttat ttttccggta tttctcaacg
     1081 aacgattagt ttaactgtgg gaagctccag acatacgatt cgagaagtta cttctcgagc
     1141 agacaggaag ggggtgactg aactaaccgc tgatatgagc gatacttggc tgtaggaata
     1201 tctttttccg gaaaaacaaa caacagcacg aggttacctt cctgtagact gggaatatgt
     1261 tcataaagaa ctaatgaaaa agaatatgac attgaaactc cttcatagag aatatggggt
     1321 acatggaaca tagaaacgaa aatgcacggt agaaaagcga atgaatatag tataaattct
     1381 acaaatcatt cgctttcttt attttatata tggttggtcg agagagacca ttttctttgg
     1441 caatttctgc aactggaact ccttgattta acatgtccac cacagtttta aagactaatc
     1501 gtttttgcgg atctgttgag ttctcagaat attcaattgg tcgcccctta tatcgaccag
     1561 cttttttggc caataatact ccttgccgct gacgttcacg gattttctca cgttcgtttt
     1621 gtgcaatata acttagcaat gataaaaaca tatcgaacat agcagtatct aggagttcat
     1681 ttccagtatt aaaatctaaa aactgtgcat ctagtatttt taatgctact tttttctgtt
     1741 tcataaaggt aactgtattt tttatgtctt cataatctct gcctaggcga tccagtgatg
     1801 caacaatgac acaatcacct tctcggatat aatttagtaa tttttgaaac tcaggtcgct
     1861 ggatatcttt accactggct ttttcttgga ataatttatc aatgttaagg tcttccatca
     1921 ttgccaattg tcgatctaaa ttttgatctt gtgaagacac tctaatatat ccaatttttg
     1981 ccataaaaac tccttttaaa aaatgatata atttacatgt aaaatttact tgtagtttta
     2041 ctgttatcat agcattttta aaaataaatt ttaagtctac ttataattta ctttggaggt
     2101 tttgaatgaa tgaaattaag cgcaataaaa attcttttgg tgtttattta ggagagccta
     2161 gctcggaaca gttaaataaa ggtgaagctc gtcatgctct atgtcgaaat atattttatg
     2221 gtagaaaagg caaactttat caaacttatt acgacggtat ggaggaacag ctcaacgcat
     2281 taggattagt aactaatgct atcatatatt ggaattcact atatttagaa aaagtctttg
     2341 aacaaattaa aacggaaggg ttcgactgtt ccgaagaagt gttacgcaag caatttccat
     2401 taattagtga tcacattaat tttgttggca aatatagttt ccgctatgaa aaagaattat
     2461 cgaatggaat actacgtccg ttaaatatag agaattattg agaatgttcg tcttttggtt
     2521 cctaccgtgc attttcgcac ggatgttcca gatactattt ttgaatgtat aaaattgaaa
     2581 acacaagaat actaataaat caacgattct tgtgttttta atttttcact accgtgcatt
     2641 ttcgtttcta tgttccatgt accccaatct tgaaaaatta attaaagact tagataaagc
     2701 cattgacgac ttagtcgtgg tgctaccgga gtatcaatgt ttaaccagta tcccaggtat
     2761 tggtcctgtt taggctgctg gaatactcgc tgaaattggt ccaattgaac gtttttctga
     2821 tgaaataaaa ttgacaaagt atgccggcct atattggcga cagagtcaat ccggtaaatc
     2881 tgaatacgaa aattttttta cgaaaagctt cacgtctaat atctatttta aaataaaaat
     2941 attcctgcta atttggaatc gtttccttct tttggtataa taatacctga aattcttttg
     3001 agaggagttg ttcgtgaagt gaaaactact atgaaggatg ttgctgcgtt agcaggagtt
     3061 ggtgtgggta cggtatctag agtaataaat ggagtaaagg tgaaagaggt taccaaagaa
     3121 aaagtaatgg ctgcaattag tgagcttaat tatgagcctg atgaatatgc cagaggtatg
     3181 aaaacgaata gtagcaatac aatagctctt attttaccta cgatttggca tcccttcttt
     3241 tccgagtttg cctactatgt agaggaagct ctcagccaaa aaaattataa gctttttttg
     3301 tgtaatgcga acggcagtgc ggaaaaagaa aaagaatata tccaaatggt caagcagagt
     3361 aaagtagacg gaattatagg tattacctat tcagacattg ataaatatgt atcatcaaat
     3421 ttaccatttg tcagtataga ccgccatttt tcggaacagg tttcttatgt aacggcagat
     3481 aattatttag gtgggcaaat tgccgctcaa gaactactta ctcgtaactg taaaaacttg
     3541 tgctatgttg ggggtgtttc accttaccct aacgaaacaa acaatcgtaa agctggtttt
     3601 tacaatattt gtgaggaaaa taaaatagct gtgaaggtat tggatatgcc tgaaccaatt
     3661 agcaatttag aggaacaatt gctcgctttt ttttcggata atcctgaaat tgatagcatt
     3721 tttaccatca atgatttcat ggctttggat gtgataaaag cccaaaaaaa actagggaag
     3781 gaagcaccta aggattttca aattattggt tttgatggag tcaagctatc gttgcaaaaa
     3841 gactatttac tatcaacgat tgtacagccg atagaggaaa tggccaaggt ttcagtagaa
     3901 acactcatgg cgatgatttt aaatgaagaa ccgcctaagc gtgtggtagt accggttttc
     3961 tttcacgctg gagagacgac taaagagtaa aaaaccggtt tataacgatt tttttgagat
     4021 aggttgacag aaagtgattt caatgatatc ttaaaaccaa ttaattggaa acgttaccaa
     4081 ttaattgaaa aataaaacgg aaacgtttca aaaaatagga ggaacatcga tgaaaaagag
     4141 aatgttagca attgcagcaa ttttagcatt aacagctgga ggacttgcag gatgtggcag
     4201 taaggagaaa agttcaggta gcagtgatcc aggagaaatt aagatttggg ttcagttttc
     4261 tgatgaaacg cctgaaggaa aagcttgggc agagattgtg aacaatttta atgatgaata
     4321 cgagggtgaa tataaagctg ttactgagta tattcctcgt agcggaagcg gcggtggata
     4381 tgaggataaa gtaaatgctg caattacaac caataccctt cctgatgtta ttacgttaga
     4441 tggaccgaat actgcagctt atgctcattc aggagtcatt gctccattag atgattattt
     4501 gaaagatgcg gacatggatg atgtattaga cagcattatt cagcaaggaa cctacgatgg
     4561 caaaatgtat gcgtttggtt tctcagagtc cagtgtgggt gtctattaca ataagtcgct
     4621 gttcaaagat gctggaatta cagaagatca attgccgacg ttagaagatc cttggacttg
     4681 gacggaattt caagcaattt gccaaaagct gactgataaa tacaaaacgc ctgcaattga
     4741 tatgcaaatg gcgtcgggag acgaaatgct gacctacgca tatacaccat ttatatggtc
     4801 gaatggcggc gatgtcgtta atgctgatgg aaccaaagcc gaagactatt ttaacggtaa
     4861 aaaatccgtt gaagcgttgg aatttattca agaattggtt gaaaaaggtt acaccacaat
     4921 cagtcctatt gaaaaaggat ttgaaactgg caaatatgcc atgatgctga gcggttcttg
     4981 gacaatggca gatttagagg aaaactatca agagattgat tatggtattt tgccttaccc
     5041 cgtttcagat gcaactgggg agttagtttc accaaccgga agctggcagt tagcaatgac
     5101 tgagaaaaca gagaaaaagg atgcttctgc ggcctttatt aagtttgcaa ccagtacaga
     5161 atctagtcgt attatgagtt tagggaacag tgttctacct atcagaaatt caacagttga
     5221 tttaattaaa gatgaagtgt cggaaggaat gcaggtatta attaagcaaa accaactttc
     5281 tggacatgcc cgcccagtgg ttgtagctta tccgcaagta tctagagcat tccaacaagc
     5341 agttcaagat attagctact tcgaggaaaa cccagatgta caaaaagtag cagatagtcg
     5401 cgctgctgaa atgcaacagg caattgatac ttcactaaaa tagaagtttc taaagggggc
     5461 caatcaagtg ttaaaaaaac cgacaatgaa agacaatttg ataggttacg gctttctagc
     5521 gccagctttg ctgctgctga cgatttttct gatcataccc gtaatcatgg taatttatta
     5581 tgcctttact gattattatt tgttaacgcc agatgctaga caatttatcg gcttagaaaa
     5641 ttttaagcga ctgttcgctg atccaatttt tgtaaaaagt atttggaaca cggctaaatt
     5701 tgttgtttgg attattccgg tccaaatagg ggcagcttta ggaatggcac ttattatcaa
     5761 caaaaatcgt aaaggaaata tcttttttaa agtagccttt tttgcgccag tcgtgatgtc
     5821 cttagtcgtt atttcgattt tatggttgta cttgttaaat cctaatgaag gtctggttaa
     5881 tacgctattt gcaaagattg gaattgcttc gcagcctttt ttgactagtc ccaaacaagc
     5941 aatgtatacg cttgttttcg tatcggcatg gcaaggtgca ggatatcaga tgctgctgtt
     6001 tttaggggga atgcaaaata ttccgcaaga cgtttatgag gcagccgaaa ttgatggttt
     6061 ttctaagttt cagcaatttc gctatatcac catgccatta ttaaagccca cggcgatttt
     6121 tattttactt accacattga tttcggcctt taaactaatc attcagccaa tggtaatgac
     6181 tcaaggtgga ccaatgaatt ctactatgac aatggtttat tacatttacc aaactggatt
     6241 tacagaccgt atggtaggct actccagctc cattgcgtta atctttacca ctatgattgg
     6301 tttaattaca attgctcaaa gaaaattggt aaaggaggat gatccagcat gaccaagaaa
     6361 tttggaccgc taatgatcgt agaatattgt ttgctgttcc ttttagcggc attattcatt
     6421 ttcccgatgc tgtggatggt agtttcctct atgaagcctg aagcagatgt ctatcaaaat
     6481 ttagcttcct tcaaagcttt tttacctagt ttgaatccag ctaattggtt taaaacttat
     6541 caagaggtta tttcccggtt cagtgtggga acttatttat taaacagtat tttttatggt
     6601 ttaacctttg cctgtgggtc aatcattgta aactctttgg cgggttttgc ctttgcgaag
     6661 gttaattttt caggcaagaa aattatcttt ggagctttgt tagctctgct gattgtcccg
     6721 atggagacgg ttttaattcc gcaatttacc atcgttaatt cactaggctt agtgaatact
     6781 cgcttggcag tgattttacc agcgatggcc agcgcgttta atatctatct gtttagaaat
     6841 ttctttatcg cgattccaga agaaatcatt gaatcagcga aattagatgg tgcaaatatt
     6901 gttaaaattt tctttcacat catgctgcca atgtctaaac cagccatagc cacagtaggt
     6961 gtcttatcct ttatctccag ctggaatgat tatatttggc cgttaatggt cttaactgac
     7021 agcagcaaat tttcaatgca agtggcaatt actacgatca atactacaca gccagtttat
     7081 atcaatcaag tgatggctgt tttaaccatt gcaacgattc cactgattat tgtttatatc
     7141 gttgcacaaa aatatattct gcagggactt ggcggttctg gaacgggtat aaaataggag
     7201 tgaatagtga atgttgcaaa aacttttaaa ggccaatgag tttattgaga aaaatgctca
     7261 aacagtcaat caagaatacc ggaataaatt tcatttgatg gctccgattg gctggatgaa
     7321 tgatccaaat ggttttgttt attatcaaga ggagtatcac ttgttttatc aatattatcc
     7381 ttacgacagc atctggggac caatgcattg gggccatgct aaaagcaagg atctaattca
     7441 ctgggagcat ttgccagtag cactggcacc aggtgaaagc tatgacaagg aaggctgttt
     7501 ttccggcagt gccatagaaa aggatggcaa gctgtatttg atttatacag gacacaatgt
     7561 tgtggaaggg caagttcgac aagttcagtg tttggcagtt tcagaagatg gcattcactt
     7621 tgaaaaatat gccggaaatc caatcatcgc agaagatcac ttagcttctg ttgcaacgac
     7681 cgaagatttc cgcgatccca aagtgtttca gcgaaaagaa gactatttta ctgtagtcgc
     7741 tactaaaact gcagatgctc gtggacaaat tgtgatgttc cattccaaag atttactaac
     7801 ttgggagttt tattccgtat tgctggaggg aactccagaa caaggcattg tatgggaatg
     7861 cccagacttg tttgaactag atggcaaaga agtcttgatt ctttctccta ttcaaatgac
     7921 tccacaaggc aatgcttacc aaaatacgaa ctcaactgtt gctttcatcg gtactgtcga
     7981 ttggcaaaca ggcaaatttc tagtagagaa ttttcatgaa atcgatggcg gtttagattt
     8041 ttatgcaccc cagacctgtg aaaacgaagc aggccaaaga ataatgattg cttggatgca
     8101 aatgtggcag cgtaaaatac ctacgaatga tctgggccat ggttggtcag gttccatgac
     8161 cttgcctaga gagctgaggg tggctgataa tcaattgttg caacggccag tttcatcaat
     8221 ttatcaagta attgatccgc aggaaaggat tgaatgggcg aagactgaag agaatcgctt
     8281 cttactgaag gacttagtat atgaccagca ctatttacgc ttggaaatca atttaaaaca
     8341 ggcaacgaaa ctagaacttc actatgcgaa gggacccgac catacattac tgcttaacta
     8401 tgatgttgaa cgggagtgct ttactgcttc cagggaacac atgccgtatg atttatcagg
     8461 agctgaaaag gaagtgttga aaaagcgcag tgtgacgatt cctttggaaa ataattgtct
     8521 aaccatcgaa attttccgtg atacttcagc tattgagctt tttctaaatg atcgtctgac
     8581 tatgagtaca actttttatg agacagaaaa aggacaggat tttcttttgc tggcagacag
     8641 tgaagtaacc ttttcattgg aaagcggctt aattaatcta tagaagggag agctcagtga
     8701 tgatttatgg ttcaatcgaa gcaggcggca caaaatttgt gtgtgccatt agcgatgaag
     8761 aaatgacgat caaggaaaga gtgagctttc cgacaacgac acctgaggaa acaatacctt
     8821 tagtcattga cttcttcaaa caataccaag cagatttagc agggattggg attggctcat
     8881 ttgggcctat cgatatccat cgcgattccg ccacttatgg gtacattacc tcgaccccga
     8941 aagtggcatg gcaaaatttt gatttcatcg gtaccatgaa aaaagaattt ccgatcccga
     9001 tttcttggac aacagatgtc aatgcggctg cctatggtga gtatgtcttc ggcagtggca
     9061 aaggattgag cagtgtcgtt tactatacga tcggtacggg tgttggcggc ggtgccttac
     9121 aagaagggcg ctttatcgaa gggtttagtc atccagaaat ggggcatatg ctcgtcgtcc
     9181 ctcatccaaa agatgacttt gctggcagct gtccatttca tggcaactgt ttagaaggga
     9241 tggccgctgg acccgccatc gaaaaacgct tagggaaaaa aggacaagag gttgcagaag
     9301 atgatcctta ttgggaaatc gaagcctcct atattgctca atgtgcctac aatacaacct
     9361 tgatgttttc tccagatgtg atcatttttg gcggtggtgt gatgaaacaa cgccatatgc
     9421 tgcaaaacgt ccacgacgca tttgcaaaat tggtcaatgg gtatgtcgaa acacctgagt
     9481 taagcaaata tatcgtgacc ccagcattag aagacaacgc tggaacgctc ggttgtttgg
     9541 cattggctcg agaagcggtc ttacattcat aaacgaaaaa aggctcttcg acatttagtg
     9601 ttggtagaga aagatacaac tgaaaattta gtcataacca gttaggcaga gtgcttaact
     9661 ggtttttgtt ttacttggaa gatcaagcct tgaatgagat aagttcttgc tcgaaagtta
     9721 tggaaatttc gatagccgta ggctacttgt attgtgcaat agttttttac aaaaaaaagg
     9781 gggtgcggat attttttttg gaccttttta acaaatatat ctttgcttct tccgatattg
     9841 aagagggctt agccttccta aagataactt aattcgttga ttattatacc aatgaatata
     9901 atcatccaat ttgtttataa actgattgat tgttgtattc ttccagtcac gttgataaaa
     9961 catctcgttt tttaatcgtc caaagaaacc ttcgcacgct gaattatctg gtgaataact
    10021 ctttttagac atagagtgaa gcaagttggc tttgttccaa tagtccaact aacaatagca
    10081 ccatcgtaac aatcgacaag tggtgataag tagattttcc ctgcgagaat tctaaattca
    10141 gtaatatctg ttaaccattt ttcatttggc ttctttgctt taaaattccg atttaataaa
    10201 ttggggtgtg caggagaaat ttccccagca tacgaagaat acttttttcg tctagaaaat
    10261 ttaacaaata gattttcctc agtcatcaat ctacgtacaa ctttttcaga aagcttttta
    10321 cccatatttt ttaaagcttg gtgaattcta cggtaaccat acgcacagaa gttatgattg
    10381 aaaatcgtga taattaattt gacgttcttt gtaatactta tctggtttct caagtgcttg
    10441 tttttgataa aaatagctgc ttttaggaaa ctggagctgt tgaagaagct ctttcaaggt
    10501 gaactgattt cgtaaggcat caatcaatat cgttttttct tgatttgata gacaaagaag
    10561 attgacgcct ttttcttttt ttagcagttc agccgctcct tcgagtaatg ctttttcgag
    10621 ctgtaattgg tgtacttgtt tttgtaagtc ttctacttcc tttaaaagta gctctttttg
    10681 tttttcctga ttttttaaag tcatacacaa tggaaaatcc tttccaagaa ccgtgttttt
    10741 ccattgatat agagtaactc tactaattcc tccagattcg gctaaatttt tcctattagt
    10801 ttgcggttta tagaattttt tcaacacagc ttctttttga tcttgtgtta actttaccgc
    10861 actttttcgg agttttctag ctcctggtgc aagttcttca caccgctgcc gcaaggactc
    10921 tctattagga taaccaagca tgcgaatggt tcgtgcataa cattgaccat attcaaaata
    10981 atgattcacc gctattcgtt tttgttcttc agaatacttt gatttttttc ggtgctcctg
    11041 atggagctct ccggattcca aatactcttt ataccatttc cgtagtgcac caatcgacgg
    11101 ataccctaat tctcgaatgg ttgcagcata agagcaatcg tattttaaaa ataactgaat
    11161 tgcccttaca cgctctttgt gggaaaacac acggacattc ctcctaagtt tattttttcg
    11221 tccaggatta tgtccgcacc cccttgcttt ctgatgactt attctggttc tttgagtgtc
    11281 aatgcataga tgtgatgtag ctagttagaa aaaaattttg aatactcttt ttttctttca
    11341 gactcctttt taagtaagat tttgaagtgt gagttacggg gtcagaaact taaaagtttt
    11401 tgatccttta ttttcaagtc tgatttggca gcttttcggc agctcattta tttatggtca
    11461 gaaatagatg ctgaaatttt aatctattag caaatagtcc agaacgtatg aagaggagtc
    11521 ggatatcatt tgtcattaac accataaata tttataaaat tcgtgttcac ctacaattaa
    11581 atttaatata taatattcgt tgtaagggct tataattttt tgttaatgaa caagatagga
    11641 agaaacattt aatggacagt tttcagagat tgtcactagt aaatcaattt gagttattgg
    11701 ctaaatttga ggaccaaaac agatcttatt atgaaagaaa aatcgaaata ttgagagaag
    11761 gttacgagta tcattatgat gatgaaattt ggagtgatct ttctgagcca tttccgaaag
    11821 aagattctcg atttgtatta gatgtattga acatgtatcg agatatcaat tttagtaaat
    11881 caaagctaaa cttagatgaa aaccaaatta ctaattcata ctatactcat t
//
LOCUS       DACGLW010000088        11836 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.092, whole genome shotgun sequence.
ACCESSION   DACGLW010000088 DACGLW010000000
VERSION     DACGLW010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11836
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.092"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            complement(558..1598)
                     /locus_tag="IXT53_002400"
     CDS             complement(558..1598)
                     /locus_tag="IXT53_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAQ4906185.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTT
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMKEDSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(1947..2273)
                     /locus_tag="IXT53_002401"
     CDS             complement(1947..2273)
                     /locus_tag="IXT53_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906186.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(2260..3048)
                     /locus_tag="IXT53_002402"
     CDS             complement(2260..3048)
                     /locus_tag="IXT53_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ4906187.1"
                     /translation="MARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVDIDPQG
                     NATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAEREFTQVG
                     SEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLKGLIKL
                     SETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKTYIRRT
                     ITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
     gene            complement(3416..3706)
                     /locus_tag="IXT53_002403"
     CDS             complement(3416..3706)
                     /locus_tag="IXT53_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HAQ4906188.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
     gene            complement(3842..4021)
                     /locus_tag="IXT53_002404"
     CDS             complement(3842..4021)
                     /locus_tag="IXT53_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906189.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            complement(4018..4368)
                     /locus_tag="IXT53_002405"
     CDS             complement(4018..4368)
                     /locus_tag="IXT53_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906190.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIIGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(4361..5686)
                     /locus_tag="IXT53_002406"
     CDS             complement(4361..5686)
                     /locus_tag="IXT53_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HAQ4906191.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKKLAQMIQENIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFVAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGIMSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGNGGIKND"
     gene            6136..6750
                     /locus_tag="IXT53_002407"
     CDS             6136..6750
                     /locus_tag="IXT53_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ4906192.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(6915..7157)
                     /locus_tag="IXT53_002408"
                     /pseudo
     CDS             complement(6915..7157)
                     /locus_tag="IXT53_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455809.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7361..8680)
                     /locus_tag="IXT53_002409"
     CDS             complement(7361..8680)
                     /locus_tag="IXT53_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HAQ4906193.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
     gene            complement(8835..9422)
                     /locus_tag="IXT53_002410"
                     /pseudo
     CDS             complement(8835..9422)
                     /locus_tag="IXT53_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809248.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(10060..10317)
                     /locus_tag="IXT53_002411"
     CDS             complement(10060..10317)
                     /locus_tag="IXT53_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAQ4906194.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(10310..10579)
                     /locus_tag="IXT53_002412"
     CDS             complement(10310..10579)
                     /locus_tag="IXT53_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ4906195.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            complement(10710..10889)
                     /locus_tag="IXT53_002413"
     CDS             complement(10710..10889)
                     /locus_tag="IXT53_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906196.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            complement(11270..>11836)
                     /locus_tag="IXT53_002414"
     CDS             complement(11270..>11836)
                     /locus_tag="IXT53_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906197.1"
                     /translation="EEQLYLESETSQTKVTVSHNRLDVYKRRPETVKKGTELLRLETE
                     KYIMNVYLERSELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATID
                     DYYLVFTRFLTCGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTY
                     TYDDTEEIQNELDRKRMPYQRFERKKRQ"
BASE COUNT         3603 a         2161 c         1789 g         4283 t
ORIGIN      
        1 gttgcgggta ataccgtaac actgaagggc acgcattaag gcgtcatctt aatgctttcc
       61 tgtaggacac tgacggagat taccagtctc ccgagcctta caggggcctt tctttttttt
      121 gtattttcaa ttatcacaat ttatcgtcca aaagtgcaga acgaaaatca attcatcaca
      181 agcatatcag acaattaaat ttaaaacaac taaaaatata caaaaaagtg taattgaata
      241 ttagcagcac tgcctgaaaa gaagtaatat caggcttttc agcttgggga aataagtgaa
      301 ttacctctct tcatgaatta ttataactca aaaaagcaca tatgcaaaca ctagttccct
      361 atttttttga atgaaaaata actaaaatat ctaacgatta tgactttaaa acctacgttt
      421 gtgattgttt tcataaactt ttaatctaaa aactccggaa taatagctat ttatttgtaa
      481 catttgtaat aaaattaaaa tcataaaaaa gttctaattt aaaattgatc atttttatag
      541 tgatcgcttt ttatcaacta ctctagccaa ttttccgtac taactgttgg taattcgtta
      601 aatgtttctc tttgttcgtt tgcaagcttt gccgcaatag ctttttcgac aaaccaattt
      661 tttacataaa cgaaaattag attctgcaaa ttgtttactt tttcagtttt ttgtttttga
      721 taagctttca gcaaagtcat atataatcct ttgtcagcat caattccata agattccagt
      781 tcttcataaa caacaactgt ttgctctaat tcttcagctt ttttctttgc attatgaata
      841 gtttggacgg ttttttgagc gtcttcgtaa gtactagaaa atgtttttat caagtttaat
      901 acattttctg gtacaaatgt atttatactg tcctctttca tgatgtcgac gaagttatct
      961 agtaaaactt gattttgtaa ctgatcagtt tcagtgtcta ttatgtgtct attagtgtct
     1021 atagaattag aatagaatag attgtctgcg attttcgcaa ttacggttga gtccttgata
     1081 tgactggatt ttgccgtatt tgcgattttc gcaattacgg tggtatcatt gatttctcgc
     1141 ggttttgccg tatttgcgat tttcgcaatt acggtggtat cagtattgtc ttcctcttcc
     1201 atgattttaa agacgtcttg attcgttact tctggcttgc ctaagtacaa taaattaggc
     1261 atggattcaa tcttaccgtc acgtttattt actcttcctc tcttctgttt cagaagacct
     1321 gctttttcta attccttttt gattttagat actttacctt cgcggcattg taacaatttc
     1381 attaattctt caactgtgaa tatgaaaaat atattatcat cagaatctac ccagttattt
     1441 ttgactgaat aatcaaatcg atctttaagt agcatataag caatttttgc atcatttgat
     1501 agatccatgt atttttcgtt agtaaaaaat accttaggca tttgataata tttttcacga
     1561 taaatttctt ttgatttgta ataattaaaa tcattcatag ccaatcctct ttctgtagga
     1621 tagcgttttg ttcaaaacta tgttacaatg aattaaacaa atatatctat ccactatata
     1681 tttttctact gctgataagt cgccaaactt tgttaaagca gtagtctttt tttatctttt
     1741 tttagtttac cttaaaaaat aaattaaagc aaaccgctct atttcaaggt ttaagctcat
     1801 agtaactata tcacgatatt acaataatat tatatcacga tatcgtaata ttacaatatg
     1861 aagataagcg acttatcagc tttgatacta ctgattttta taaaaaaaat atcacgatat
     1921 tatgatatcg tgatattgaa tgtaaattaa attttatttt gtttctttgc tagtttatat
     1981 ccttcaatca agtattcttc aggtatggac tttaatagcg catcttctat aacgtctggt
     2041 ttacttttgt ctgattcagc aatagtcgtc caatatttga gtgctgacat tagaattgga
     2101 tttatacgaa tgctatctgc tgttcttaca ctacttcctt gatcttttat gactttactt
     2161 atatttttct tggttaattc gatttcttct acttcttttg atttaaaatt ctttttattt
     2221 ttatttccta gtaatctact tactgcagct tgactgcgat tatcttctgc catcaaaatc
     2281 accaacttct tttaaatatt cttctgtaaa tgctttatag tcatcttcag ctgttgaggg
     2341 tttattaaaa ttgattagat ctgatccagc agcttgcgct tcgtcaacgg ttattgtcct
     2401 acgaatataa gttttgaaaa tttttgcacc aatgactcta gaaatttcgg ttgctgcaga
     2461 cgtcatttct ttagaaatgt tataacgctt attatactta gtaaatagaa ctcctcgtac
     2521 ttcaagcttg tcgttcgtat attcttttac cgtttcaata gtttcactta actttattag
     2581 tccttttaat gagaaatacg cagcttcaac tggaatgata atttcatcag aaacagtaaa
     2641 tgcgttgact gtaagcattc ctaaacttgg tggacaatca attataatat aatcatagtt
     2701 gtccttgatt ggttgaagta ctcttttaag cctgtgttca cttcctactt gagtaaattc
     2761 cctctctgca gaagaaaggg ctatgtcggc tggcaaaaca tcaattcctt tatagtccag
     2821 aatagcatct gtaatgtcag cctccccttt taatacttca taaattgtag gttggtttgt
     2881 tccatctata tcgaaaccta cattatctgt agcatttccc tgaggatcaa tgtccactaa
     2941 aagtacattt ttaccgtaaa caaatttcaa acatgctgct aaattaacgc tagtcgtagt
     3001 tttagaaact ccaccttttt gattggctac tgacaatatt cttgccatta aaatatcccc
     3061 cattcatctt atattataat ataattatat cataatattg tgatattgca atatcgtata
     3121 tgcattatat tgtaatattg tgtgattatt ttgttttaaa gatatatatc ggttcggata
     3181 taaagattat tcatatgtta ttcgaaatta aaataatcac tttttattac tttaaaattt
     3241 tttattaatg ataagaaata tgttttgcga gcagcaacta gaataattta aacattacta
     3301 aactgtctat attgttacta ataattccct tcttatagtg cattttctta gaaaaaaaca
     3361 atattacgat atcatgatat taagatatcg taatattata caattgtgat tgatattata
     3421 tatgtttttt tattttttta tcatgtttaa tcttgaagga atcatattct ttttctaaag
     3481 acaggagctt ttcataaaca tcagacatgt ttaaactaga ggccaggctt aaatcataaa
     3541 cgaatgaagc aggtaatgat tttattctac gttgtcgggt tatccagctt gcaaccgttc
     3601 cttgtgggta ggcatataac tcacaaaatt gttctacagt gattcctaaa ttcttgataa
     3661 tgtaaacatt gatcgggtgc gagtaaacaa atgtattgat agacatggtg cgtaagccta
     3721 ctttctttta ttattgcgtt tgcaataata gtttaacaca cgcattattc tagtatctac
     3781 tcctatttgt aaaaacaaaa aaggattcat agagataact ctatagaatc cgtaaaatac
     3841 cttatatata taacgggaag atataaaacc taaaattatg ataacctgct gcagcttcta
     3901 catagaaaga gaagcgctca acaccaacct catttatcaa acgttggcca taaccccaaa
     3961 tctcttctga tagctgatca aaggttatat actttagctc atattgctgg ataatgttca
     4021 ttctaatcga ctccatttat tatcctggta aaattcgata tgacgtattt cgtcgtagtg
     4081 aattttgtct tttccaacgt agatccccag ctcatcacta cctaagatct tgccaatgat
     4141 atcttctgga tagtatcctt cagcgtttcg ttcttctttt tgaatagata ctgatctatt
     4201 ttttatgact gcagcgttca atacttccgc aatctcttca gtcgtcattt gttcttttgc
     4261 taaattttga ctggttcgtt gttcattgtc ggcatcaatt tcttcagtat gatcagataa
     4321 ataaaaccct gcccacttta aacgtccacg atcattgtaa tcaatcattt ttaattcccc
     4381 catttccgcc cgcatgacca ccaacaagtg tgcttctagt aatgcttcta gcgccttcta
     4441 acattgaaga agcatgtaca atcgacgtaa aaccatattt ttgccgaatc ttgtcaataa
     4501 ttttatctaa attttcttcg tcaatttgct tttgtggatc actaaataaa tccagctgaa
     4561 gcgaatcggt gtacacaagc ttgctatagg ttattccaac atgccttact tcttgtcctt
     4621 cataatactt atcaaacaag aaaagacaat aattctgcaa ttcctttgta ttatctgtga
     4681 tagggatctt catctggtgt gaaaaccctg gtctcgtttc taagatcgac gtgccaatgt
     4741 gtaaatggac acaacctgtt ttacagttat gctgcctaat gcgtatcgct acttgttccg
     4801 ccatttcttt taccaccagc tcgatttcat ttcttctagt ataatctcgt ggtaacactt
     4861 gtgaatttcc atatgatttt tcctttgtcg gttctggtgg gattgctata tctgttcgat
     4921 cgatcccatt agcatggaag tacagctgta agccaatcac acctagtcta ttcttaatcg
     4981 tataaggatt ccagttagca agatctctaa tgctcataat ccccatctga ttgagtcttt
     5041 ttttcattcg agagccaatt ccccaaaagt ccgtcatttc aggaatattc catacttttt
     5101 caggaacgtt ttcgtacgtc cattccgcaa caaatccttc attatgcttt gcttcgttgt
     5161 ctaaggctag tttagccaaa aggggattat ctccgacacc tactgtagca atcaatccta
     5221 gctcttcttt aatattctcc tggatcattt gagccagctt ctttctccgt tgacttcgtg
     5281 ttccttcagt cgtaaaaaga ttcagtgatc gggtcacttt taagattgat tcatcgatcg
     5341 agtaaatcag tagatcttca tcagccacat atcttctgaa aatattattt acctgcatat
     5401 ttcgcttgat atataggttc atacgtggtg gaacaacgtg tagtgtttta ggaaatggtt
     5461 gtggtaagtc acgtggtcta ctcacatttg taataccata ccgctttttt gcttcaggag
     5521 aagaagctaa tatcaatcct gaaccagtat tatcagctcg actcataaca acaagttctg
     5581 ttgttaatgg atctaaattt ctttctatac attcgacact tgcgtaaaag cttttcatat
     5641 cgattaaaaa gtaatcattt actggctctt ttgaataatc catcataaat aacactccat
     5701 tctttcacat tatacaaaca tatgttcgat attgtaaaga agaaatagct aaatacataa
     5761 aaaaagaagc ataggtatgc tatcaccctg cttcttcttt tgatttgtat gatcaattta
     5821 ttctatcact ttactaatta atttgaaaat ctaataaaaa ctctcactct aaaagagtga
     5881 gagactgcaa attagagtac ggactttttt ggagttccgt tctttaatat taacaaatga
     5941 ataaaatcct tacaacattt gagaataagt tatcagtaga cacttacaaa aaattctcat
     6001 actatttcct ccatcattag tttttgtcta tataaggttt accctaaata gacactggta
     6061 taacattgaa aaatatggct tcctcgtgta tgattagggt gtattttaaa tagttgcaga
     6121 aagtaggtaa aagttttgag agttgcatac atacgtgtct catcgatcga tcaaaatgaa
     6181 caacgccaga ttgaagaaat gaaaaaattc ggtgcagaga gaattttcat cgagaaacaa
     6241 tctggcgcaa cgattactca tcgacctgtt ttccaagaag cattagatta cgtccgagat
     6301 caagatattt ttattgtgga agctattgat cgacttggaa gaaattatga cgagatcatc
     6361 gcttcagtca attacttgaa gaagaaaaat gtgaagctga tcattactag tttaccgatc
     6421 atggctgaag caattggcaa tcctctcctg gataaattca ttaaggatct gatcatccaa
     6481 attttggcca tgatcgctga acaagaacgg actgaatcga aacgtcgcca ggcacaagga
     6541 attaagatcg caaaagcaaa cggcgtctac aaagggcggc ccaaattata cagtgcagac
     6601 gcaaaagatc cccaacgtcg ccttgtttac aaaagcatcg ttgaagatct taaaaatggt
     6661 gttgcgattg ctaagattgc taaagactac aatgttacga gacaaaccgt gtatagaata
     6721 aaaaaagaca gcatggtcaa tgacaaatag ttgatcatgc tgcttttatt atcttctcat
     6781 ctttaatttc ctacatataa ttactatcta taaaattaag tcttactcag aacagataaa
     6841 gcataaaatt ccaacaaacc attttttact taactaaata aagagtctgt taatataatc
     6901 ttcacttatt tacgttaatc ccctactgta tttgttggtt ccttatctat attttctaca
     6961 aaatccttat tatcttgtga tttagcttcg tataattctt tgtatgtcga cacattggtt
     7021 atactaattc ctatagctag ttcttgagac tctacttttc tctctagctc ctttgacaaa
     7081 gtgatttgag atttatttgg ttccgttgga gtcaattcta tagattcttc ataaaaatct
     7141 ctatacataa agctatccct gaataattca tagctttaat tctttggcac attttattga
     7201 aatatgtatc actaatatcc ctatcaactg attttgacta aaaagttact tcaaacattc
     7261 actgttcaaa aatattgagg aataaatttt gggtgtaagg cattttgagc atacttctcc
     7321 ctggataact tcccagtaaa aaaatcgtta gattgttgga ttagatccat tgactggctc
     7381 gcctcaggcg agcaaagacc ctgtagaatt cattctgata cacatgataa ctagataatt
     7441 tgagatagac tcgtctaccc gtttggacta atttcccagc aactttcaga aacttcaatc
     7501 gaatagaatg aatggtcatt cccttttgga cttcttcaaa gccaatcgtt cttaagaagt
     7561 tgactaagtt gtaagctatc aagctgagca tcattcggac atgattctcc aaaaagcgag
     7621 gactgtctgt tttgtcaaag taaaagccag ctttcgcttc tttaatgaag ttctccattg
     7681 tgccacgttt ggcatagata gaaaagatgg tatcaggaga aacattttct gatagattcg
     7741 tcacgataaa ttcatgtcga aagagtagtt cgcccgcttc acgtgttgag cgtatacaca
     7801 cgcgacgact ttgtgaccag gattgtgctt gataagtggt cgagaagtac tgaacttctc
     7861 gttcttccca cttttgatta tcgccataaa gtactgattt ctcagctatt tgacctagtc
     7921 tacgattatt cttcagtcga ataacataat gactcttttt tgattcacac gaatcataca
     7981 caccaggtgt agcgaacccg ctgtctccac gaaccaagat gtcagtgttt ggtagagagt
     8041 gattatagtg ctctaataaa ggtgtgagaa actcctttac gccttttgat gtatattgat
     8101 ttcctgaacg tagttcagct tttaagaagt caccagtcaa tccgtcaaaa gctaccaatg
     8161 gatggtaacc ataggtttgg tagtgtgtat tataattcgt ttgttcttgg tgaccaaatg
     8221 tatctgaatg ggtcgaatct aaatcaatga ttaattccgt atcattacgg atgaggcgtg
     8281 ctttatcgat aagttcttgg ttcaaagctt gaagttcatg gatattctcc tcagatagtc
     8341 gatctaaaaa tcgggaaagt gaagattgag aagcgagttc tttcctatct aaaacagctt
     8401 taaacacagg atcttgtctc aggagattag ctgcagaatc agctgagtaa ccagcaatta
     8461 attgcataat gaactgctca agtatggata agttgtcatg agtaaaatac gctcgttcgt
     8521 cttcaatgtg aatgtgttgc ttagccagtt cagaaaaacc aaagatgttc atcagctctt
     8581 tcactaggac gagacccgaa tcactcgata attgaccacc tgtatgagaa atagtgatat
     8641 ttgaattgaa ttttacttgg tttttgtgta agctagtcat gaaaagaact cctttctttt
     8701 ggttggttta gtcactttaa ccatagcaga aaggggttct tttttcatca cttaacgggt
     8761 gaagatgaaa aagtaattgt aagctgccgt gaggcagttt cacatggttt taattaaaag
     8821 tatgaattat tcaggctatc attaatttta taaaaaaaat taaatgtttt tagcactgcc
     8881 atataacatt caaaacattc ttctatagtt ggtttacttc cttgaggaga atgaaccaat
     8941 ttattacgtt tttctatgaa atttttaatt tcctttctta tttctttcgg aaactcaata
     9001 ttaaagtacc ctttaaacat gctccctaat aatttgtcta cagggggaga ttgtaaattc
     9061 tcaaatatta gagttaaatc aggtttctct tgttgataaa attcctttac ccctatttca
     9121 caggctgtaa ttatcgaaat aaacctatct ctagtatttg atatattttt acttctttta
     9181 aggagtataa gagaaaaggg gatactttcg tgtccatgca tgtaattaac taattgttga
     9241 gcttcagaag ttatatctct aattatatag ttaacggaat taggaagtgt ctttaactct
     9301 aatgaaattt ctatccaatt actatcttct aatctgtata tgctcgaagt ataatttgat
     9361 tcgtcagcaa gcatcgctac atcccaaaca ctgatcataa aatcttttaa acgttcaatc
     9421 atttcagcaa tattgctaaa atcagctaat ttttctttag aataaaaaaa aacatttata
     9481 tcagaatcaa taaattctat ttttttatta ctatatcgtc atcattatct tgaccattta
     9541 tgtttattgt tccctgagtg gggagtactt ctttcaaatt caagttaaat ttatattcca
     9601 ttacttctcc tccaatatat aaaaattact taactgtatc caaatttaat atgtaaataa
     9661 atcattaatt tttagctaaa ttctatgaga agtttatttt tgttgttttt gctaattaac
     9721 taaaactagt acaaaaaatt taaaatagat tattaaacct attgcttatt ctattcagtc
     9781 atatatataa gcgtcatcct ttagtaacta acatcccaca ctacaatagt attataacat
     9841 atctattcaa cttcaaattg ttgaaattta agccatttat atttactttt cctcaacttt
     9901 agaaatgatt ttactaacct tatatccgta atacgcgtaa caaacacatt cattaataac
     9961 aatcaacact aagctatatt agctttttaa atgactaggg taaataaaat aaggatcaaa
    10021 aacttttaag tttctgaccc tttccttact tccgattggt taatagtgat cttttgcaga
    10081 ataaataaat atcgtttcat tttcaactct atatatcagt ctatgttcat ctgtaattct
    10141 tctggaccat tttccagata aatcatgctt taatggctca ggttttccta atccagcaaa
    10201 gggggaacga tcgatatctt ttattaactt gttaatcttt tttatattgc ttttgtttcc
    10261 ttgctcatgc caataaagat aatcatccca agcatcatca gaccaagcct taatcatcag
    10321 attcaacctc gattaagtca tgtgttttaa atgcaccttt ggagaattgt tcatctcctc
    10381 gacgaatttt ttccatgacg taattattag aaagtgttct caacgtttct tgcatagaat
    10441 cataatctct ttttgataat acaacaactg tatcttctac atctttactt gttacaataa
    10501 gtgtttcagc atcctcatta acttgtttca tataactacg taaattttgg cggaaatttg
    10561 aataagctac tgcttccatt cctttcaccc cttatttatt tctataaaac aattgtacat
    10621 tatattgtac aattaagcaa atgattaata ttctaacttc ttataattaa aagtgctatc
    10681 acttttagtt tttaggataa aaagtgacgt cactttttga gtgctggtac ctcaaatcct
    10741 gctttttctt gggcaatcaa taaatctaac gcaatacaca taatcctgga ttcacttttg
    10801 ccgtacttct gctttagttg ctgcaattct ttttgtctct tttcatccaa cgtaacgggt
    10861 aaccgtttat ttcctattgc aattggcatt agtactctct cctttcatcg aaataaccct
    10921 atgaaaaata tggctgcaat catcttgaat agtagactca caagggctaa tacaaagcgc
    10981 caaaagatca aacaaaatac cagtattgcc aaaaatacta cgaagtttaa atgcacagct
    11041 gcagcatggc gcataagtat ttcgccaaca aataggacta acaatagagg aatgatttta
    11101 ttcatatgga tctccttctt tctcttaggt tatcgggtgt tgcccgctta tttcgcacca
    11161 cctaagcggc gggggcggtt ggagcgattg gtgcgaaata agaaccattc ccttagttac
    11221 taggtttttt gtccttattt cacacattct cacttttcgg tataaatgat tattgccttt
    11281 ttttcctttc aaatcgttga tatggcattc gtttacggtc aagttcattt tggatttctt
    11341 ctgtatcgtc gtaagtatag gtgtctaaaa aatcagttaa agtcgtgtac cagtcgtcgc
    11401 ctgggttatc tacttcttct acatattccc tcaaaaatgc ttcgggtaca gcgagttcat
    11461 aatccattcc cattaacacg atagattccg ttccacacgt caaaaatcta gtaaatacaa
    11521 ggtaataatc gtctatcgtc gcatctttgc tgtttagctc ttccatttct tcttccatta
    11581 attccatgct ctcaaaaatt gaataatatt ctgattctga gtaatagtgt ttcctgcaaa
    11641 tcgtgtagct gacaaagttt tcgaccagct cactcctttc taggtaaaca ttcatgatgt
    11701 atttttctgt ttctagtcgt agaagctccg ttcctttttt taccgtttca ggacgacgtt
    11761 tatacacgtc taaacgatta tggctgactg tgaccttcgt ttgactcgtt tcactttcca
    11821 aatacaattg ttcttc
//
LOCUS       DACGLW010000089        11760 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.093, whole genome shotgun sequence.
ACCESSION   DACGLW010000089 DACGLW010000000
VERSION     DACGLW010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11760
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.093"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            complement(51..926)
                     /locus_tag="IXT53_002415"
     CDS             complement(51..926)
                     /locus_tag="IXT53_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAQ4906198.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(1008..1799)
                     /gene="codY"
                     /locus_tag="IXT53_002416"
     CDS             complement(1008..1799)
                     /gene="codY"
                     /locus_tag="IXT53_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HAQ4906199.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(1817..3217)
                     /gene="hslU"
                     /locus_tag="IXT53_002417"
     CDS             complement(1817..3217)
                     /gene="hslU"
                     /locus_tag="IXT53_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HAQ4906200.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(3231..3779)
                     /gene="hslV"
                     /locus_tag="IXT53_002418"
     CDS             complement(3231..3779)
                     /gene="hslV"
                     /locus_tag="IXT53_002418"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HAQ4906201.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(3816..4721)
                     /gene="xerC"
                     /locus_tag="IXT53_002419"
     CDS             complement(3816..4721)
                     /gene="xerC"
                     /locus_tag="IXT53_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HAQ4906202.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(4919..6295)
                     /gene="trmFO"
                     /locus_tag="IXT53_002420"
     CDS             complement(4919..6295)
                     /gene="trmFO"
                     /locus_tag="IXT53_002420"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HAQ4906203.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(6264..8342)
                     /gene="topA"
                     /locus_tag="IXT53_002421"
     CDS             complement(6264..8342)
                     /gene="topA"
                     /locus_tag="IXT53_002421"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HAQ4906204.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(8464..9321)
                     /gene="dprA"
                     /locus_tag="IXT53_002422"
     CDS             complement(8464..9321)
                     /gene="dprA"
                     /locus_tag="IXT53_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HAQ4906205.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(9377..10144)
                     /locus_tag="IXT53_002423"
     CDS             complement(9377..10144)
                     /locus_tag="IXT53_002423"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HAQ4906206.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(10137..10997)
                     /gene="ylqF"
                     /locus_tag="IXT53_002424"
     CDS             complement(10137..10997)
                     /gene="ylqF"
                     /locus_tag="IXT53_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HAQ4906207.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            11257..11655
                     /locus_tag="IXT53_002425"
     CDS             11257..11655
                     /locus_tag="IXT53_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HAQ4906208.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
BASE COUNT         3102 a         2557 c         2060 g         4041 t
ORIGIN      
        1 tacacacctt ggtaaacggt gttccctgct aacttctcgg tcacaacctc ttacttcact
       61 gtgatggaat attttgtctt gaagatttca tgggcaccta gttgattgat ccctactttt
      121 tccatcaagt ttccactact gttcaccgtg tcagcgattc cgcaccacgg ttcgatacaa
      181 acgaaaggcg cttcttttgg ataaggagac cagatcccta cataaggcat atctttatag
      241 cttaaagtta cgctatggga agatttttca cttcgaatgg cgtacgcatt tagacccctt
      301 gtttcaaaaa ccaaggcatc ttgctcaaaa agttcacgat tcaaagccag attcgtattt
      361 gtctgaccta acgtcttttg atccatatcg atgaatgggc ctttcaatgg taggcgtaat
      421 cttgatttca atggagaaaa tgccagatag aaatcttcaa atgacaatcc ttcttcaaga
      481 ggtacattga aagcaggatg tccaccgata gaaaagaaca tttctttttc accggtattt
      541 gccacttgat aatgaaccgt gatgccgtct cctcctaatt catagctgat cactaattca
      601 aaatcaaatg gatacttttc taatgtctcc ggcgtactcc ttaagatgaa actagccatt
      661 tcttgtccat gttcaatgac ttcaaaatcc atatcccgag caaatccgtg ttgccccatt
      721 tgatatgttt tcccttgata aacatactga tcatctttta aacgtccaac aaaagggaat
      781 aaaaccggtg catgtctgcc ccaatacgct ggatcacctt gccagatata ctctaaacca
      841 tgttcttttg atttcagact gatcaattcc gcaccgtgtt cagcgatttc ggcgatcagc
      901 tggtcatttt gaataataac agtcattgcc attcctccaa cttcgactta ctttttaaaa
      961 tgggaaggct ggaacaaaca tttctatctg ccccagtccc cttacttcta ataactgtgt
     1021 tttgctaact cgtctttaaa acgggaattc aatactttta aataagttcc cttcatgcct
     1081 aacgaacgag attcaatgat tccagctgat tccaattttc gtaaagcatt gacgatcaca
     1141 gaacgagtga tgccgatact atcggcaata ttagaagcag tcagacgacc ttcatctcca
     1201 tccaatgctt caaagatagc ttgtacagcc ttcaattcgc tataagataa tgtgttgatt
     1261 gccatttgga cagccgttgc actccgtaca tctgcttcga tgcttcgaga cttctgataa
     1321 aggatctgca taccgacaac cgtagctcca tattctgcta agactagatc gtcatctgca
     1381 aacgattgtt ccatacgaga taagataatc gtacccaaac gttcgccagc tccaaagatc
     1441 ggaacgacag tcgtcagacc aaatggatat ttctctcttg actctaccgg aaatgcagta
     1501 atatcactag taattgggat attagcttca gttttcatca actgtgccac tgtatcagta
     1561 taagattcag gaaattgttt ctcaacaaac attgttttga ttcgttcatt gttcacatcg
     1621 tgacgttcat tataacctag taatactccg tcacttccta tgatataggt gttgctgtca
     1681 agaataccac ctagtgtgac agccatttta tcgtaaggca aatctgcttg catgtcaaaa
     1741 gtattctttt gctgcaaaag tgtgtttatc ttgcgggttt tcgttagcaa gtctgtcatt
     1801 gtcgtcctcc gattttttat aaaatatagc gacttaaatc ttcatttgcc gcaattgagc
     1861 caagtttgtc atccacatat gcttctgtga tcgttatttc tcccatttgc atatctggtg
     1921 cttcaaataa tagatcttct agtagttttt ccaaaatggt atgcagtctg cgtgcgccga
     1981 tattatctgt gtcacggttg acatcgaaag cgatttgagc gatccgttca attgcttctt
     2041 ttgtaaacgt aactgacaca ttttctgtac cgatcaacgc gacgtattgt tttactaaag
     2101 cattatttgg ttcggtcaga atcttcacaa aatcttcagc tgtcaaatca tctaattcta
     2161 cccgtatcgg gaagcgtcct tgaagttctg gaattagatc acttggttta gacaaatgga
     2221 aagctcctga agcaatgaat aggacgtgat ctgtttggat gatcccgtat tttgtgttta
     2281 cttgtgaacc ttcgacaatt ggcaatatgt ctctttggac gccttctctg gatacttctc
     2341 cagattgctg tgatttagaa gtgatcttat cgatttcatc gataaaaata attccacttt
     2401 cttccgctaa tttgattgcc tcactatgga tatcagcatc cttgactaat tttgctgatt
     2461 cttccttgat caataactct aaagcttctt tgactgttac cgtccgttcc acttttttct
     2521 tcggtgtcaa agcacctaga gtttcattta ggtcgatccc catttgctcc aatccattgt
     2581 tcatcattgg tgcaggtttc ttttggtctt caacttcaat ggttacttca cgactatcca
     2641 gtaaaccctt ttgtagttgt tccaagactg tacggcgatt gattcgaata tcttctgtca
     2701 cttcttcttg cggttcagcg gtattttgct gactagcatt aaaaagattc atcatttgtt
     2761 caaattgatt agtggattgt ttctgttctt ttttgatacc aggtaccaag atttttacca
     2821 aacgtttttc tgcatttttt ttagcacttg tgtatacacg gctgtactgc tctttttcta
     2881 cgatttggat cgcattttct actaaatccc gaaccattga ttctacatct cttccgacat
     2941 aaccgacttc agtaaatttt gttgcttcga ctttgacaaa tggtgcattg acgatttttg
     3001 ccaaacgtcg tgcgatttct gttttaccga cccctgtagg tccgatcatc agcatatttt
     3061 ttggtgtaat atcttgttgc attttttcat ctaactgcaa acggcgatag cggttacgca
     3121 aagcaacggc aacagatttt tttgctgcgt gctgtcctac gatatattca tctaattcac
     3181 ttacgatttg tcttggtgtt tgttttaatt catacatatc tactcacctc ttacagttct
     3241 tctacgataa tattatgatt agtaaagata cagatatctg cggcaatatt taacgcattt
     3301 tctgcaattt ctttcgcact catgtcgttt tgagcatagt ttttcattgc tcgggctgca
     3361 gccaacgcaa aattccctcc tgatccgata gctaagatac catcatctgg tgtgataact
     3421 tctcctgtac ctgaaacaag aagcatttct ttatcattca ttacgatcag catcgcttca
     3481 agtttttgca tagattgctg tgtccgccat tcttgagcta attcgacagc tgcgcgttgt
     3541 agattcccgt tatattcgtt tagtttgcct tcaaattttt cttctaaagt aaacgcatct
     3601 gcaacacttc cggcgaaacc tacaacaact tctccattgt agatacgtcg tactttcttt
     3661 gcagtgcctt tcatgacaac agattcaccc attgttactt ggccatctcc agccatagca
     3721 aatttcccgt ctttttcgac ggcacagata gtggttgaat gaaatggtga ttcgaccatg
     3781 tttgtttcct cctaaatttg ttcttttcct tatttttacg ctcttggatg gaaagaacga
     3841 tagttttttt gtaaactttc ttttgttaca tgggcataga tctgggtagt cgataaattg
     3901 gcatgtccta gcaattcttg gactgtccgc aaatctgccc cattatttaa taaatgagta
     3961 gcaaatgtat ggcgcagcat gtgaggatgg atatcactat ccaaactgct ttttttgatc
     4021 atttgattca agacatattc aattccagta ggcgtgattg gttttccatg atgattcaca
     4081 aaaacgatat catgctcttc gtgatatttt gtcatcaata cctggcggcc ttccctgaaa
     4141 tattcttgca gagcatcttg agcaaaagaa cctagaggag catatctctc tttgttccct
     4201 ttaccatgga tcaacatgac ctctgaatca aaatcaatgt ctgataccga caaactgcta
     4261 cactcactta aacggatgcc tgacccgtaa aggacttcca gtaaagcgcg gttcctcaaa
     4321 tccaaaggac tatctcctgc tacgctgtca aaaagtgctt gcatttcatt ttcataaaaa
     4381 aaacgtggca gtctcaaatt tttctttttg agatgaacgt aagaaaatgg attttctttt
     4441 atcacttctt ctttgagaag atactgataa aacgaacgta ggctggctat cttgcgactg
     4501 atagaatttc tgctatagct aggacgatga tcagctaatg cactcaaata gacacgaacc
     4561 gtaaaatatt ccgccttcag cagatcagcg tctccagatt cctccaaaaa atgtttgaaa
     4621 tggagaatgt cttcttcata tgcttctttc gttttttctg aatatcctct ttcgacaatc
     4681 aaataacgca aaaatttttg ttcccacgtg gcttctgtca ttttctcacc ttccttttgt
     4741 cccagagaat aatttagcac agcgtttata aaaagacaaa taaattgtca gaatttaacc
     4801 aatatttcta agtttgttca caattggcgc aattcctcac attttgtttg agaaattctt
     4861 aagaaaaacg caagaaaaag agcgagacta tccgtctcac cctcttcatg gagtttcctt
     4921 actcatttgg gattgcagtt ttttctaatt ctgtttttat tgcctcatta gcttttaaag
     4981 ctcgttgtgc aattgcttca tatcttgctt ttttgtctcg aatgcgctct ggcagttcag
     5041 gaaacagacc atagttggca ttcatcggtt ggaaatgttt tccttccgca tgagtaatgt
     5101 agtaagccat acttccaata gctgtttctt gtggaaactc gactggttcc atttcttttg
     5161 ctaagcgtgc ggcgttgatt ccagcaagta gtccgctcgc tgcactttca acatagcctt
     5221 ctacaccagt catctgacct gcaaagaaca gatcttcgcg tttcgttgat tggtaagtcg
     5281 gtttcagcaa ttctggcgaa ttcataaagc tgtttcggtg catcacacca tagcgaacga
     5341 attcagcatt ttctaaccca ggaatcatcc ggaaaacacg tttttgctcg ccccatttca
     5401 agtgagtctg aaagcccacg atgttgtata aagaagctgc agcattgtct tgacgcagct
     5461 ggataacagc ataaggacgt ttgccagttt taggatcttc caatccaact ggtttcatag
     5521 gtccgaaaag catcgttttg aatcctcgct gtgccattac ttcaattggc atacatcctt
     5581 caaaatattt ttctttctca aaagatttaa gttctgccac ttcggcattg attagtgcct
     5641 catggaaagc tttgaattcc tcttctgtca tcggacagtt caagtaagct gcttctcctt
     5701 tgtcatagcg ggacttcaaa tatactttat ccatatcgat cgtagctttg tctacgattg
     5761 gtgcagctgc atcatagaaa taaaaaccat ctgaaccatt gaatgctttg atgctttctg
     5821 ctaatggatg agaagtcaaa ggtcctgttg ctactacggt gattccttcc ggtatttcag
     5881 taatttcttc gtttatcact gttactaatg gatgctcttt gacttttcgg gtaattgttt
     5941 cagaaaaaga atcgcggtca acagctaagg caccaccagc tggcactgct gtttcatctg
     6001 ccgaagtgat tactaccgaa ttcaatcggc gcatttcttc tttcaataac cctactgcat
     6061 tagccaaact gtttcctctt aatgaattgg aacatactag ttcagcaaaa ttacttgtgt
     6121 gatgcgcctc agtcgatttg actggacgca tttcatataa acggacaggg actcctgctt
     6181 gtgcgatctg ccaagcagct tcacttccgg caagccctgc tccaacaaca tttaccattt
     6241 tcaatgaaag aacctctttt ctattatttt tggatgtctt cttcgtaatc tccatttaca
     6301 cagactactt gtttaccgcc tttgactttc ttctcgacca gatatccgtc acattttgga
     6361 caatttctgc ccactggttt atcccaagaa gtgaattcac attctgggaa acgggaacaa
     6421 ccatagaata gtctgttttt cttagatttt cgttcaatga cttgtccttc atgacatact
     6481 gggcaagtca caccgatttc ttttacgatt ggtttcgtat tccgacaatc aggaaaatta
     6541 ctgcaagcat aaaacttccc atatttccct aattttatca ccatcggatg tccacagaca
     6601 tcacaatcga atcctgctgg ttcatctttg atttgaattt tttcgatttt ttcttccgca
     6661 ttcgtcagtt ctttttcaaa cggtttatag aaacgatcaa ccactttgac ccacgcttcc
     6721 gtgccttctt caattttatc caaatctcct tccatttctg ctgtgaaatg gatatcgacg
     6781 atttgaggga agaattcaca gattaatgaa ttgacaattt ctcctagttc agtcggctca
     6841 aatctttttt gtgtcaattt tacatagtag cgtctttgga tcgtatcaag tgttggtgca
     6901 taagtggaag gtcgccctac cccgttttct tctagtgcac gaatcaacgt agcctcacta
     6961 aaacgtgctg gtggttgagt aaaatgttgt tttggttcaa tatcaacaga ttggactaca
     7021 tcgccttctt caagttctgg aagaatattt tctttctctt ctttgccgtc atctctacct
     7081 tccacataca cttgcatgaa tcctttgaat ttgatttttg aaccgttcgc aatgaagatg
     7141 acaccatttt gttctaatgt cactttcatt gtatctaaaa tagctggtgt catctgacta
     7201 gccacaaaac gtgaccagat caatgtataa agtttcaatt gatctttatc taaatattgt
     7261 ttgagttcat ctggtgtacg aagcacactc gttggacgca cagcttcatg ggcatcttgt
     7321 gcgccttgag catttttgat ctttcggtga tcatgtctag aaaattcttt tccatacgta
     7381 tcttcaataa atgctgcggc ctctgctttt gctgtatcgg caatccgggt agaatcagta
     7441 cgcatgtatg taatcaagcc gactgttcct tgtttaccca aagtgattcc ttcatataat
     7501 tgctgtgcta ccatcatggt ttttcttgtt cgaaagttta acttacgggc agcttcttgt
     7561 tgcaggctac ttgtagtgaa aggattcgca gggtttcttt tgcgctcttt cttttctact
     7621 tttttaactt cgaagtctgt tccatcaatt ctagcagtga cttcttttac actttgtgca
     7681 tctggtaatt tctttttctt accgtcgatt ccccagaaat ttgctttgaa tttcttgcga
     7741 gcttttttaa aattaccgtc gatgctccaa tattcttctg gaacaaattc tcggatctct
     7801 ttttcacggt caataataat ttttaaagcg acagattgga cacgtcctgc acttaaccct
     7861 ttcttgacct tacgccagag aataggactg atcgaataac caacgattct gtctaaggta
     7921 cgacgagctt gctgtgcatc tactaaatct acatcgatcg ttcttggttc cttaaaagct
     7981 gctttgactg cgtcttttgt gatttcattg aagaccaccc gatttttttc tttcagatcc
     8041 aacccaagta ggtacgctaa atgccaagca atcgcttctc cttctctatc cgggtcactt
     8101 gccaagtaaa ctttttgagc tttttttgct gcggctttca ggcttttgat gacatcgcct
     8161 tttccgcgta tagaaatata atggggttca taattgtttt caaaatcgat acccatttta
     8221 cttttcggta aatctcttat atggccaaca ctggccatta ctttatagtt tcgtccaaga
     8281 tatttctcaa ttgtcttggc ttttgcaggt gattctacaa tcactaaata tttatacgcc
     8341 attactgaat ggactccttt ccattgtcgt gttcctacta ttaaaagagg attcatacaa
     8401 atcgtagcta atcatatcca ttcagaaaac gtttgtcaag aaaagcaaat gaaaagtatg
     8461 gtgttattct ttaaattctg ccaagatatc ttcacccgaa accgtgcaaa ctgcaccgtc
     8521 ttggatcaaa tgatgacatc cggtagaatg ttctgttaaa atatttcctg gaacagaaaa
     8581 aactgttctt ccaagatcca atgcttgctg tgctgttatc agagagccgc tttttttctt
     8641 ggcttcaaag acacatgttc ctttagacaa agctgcgata atccgatttc tcattggaaa
     8701 acaatgcttt ttcgggccgg attgaggagg atattcactc aaaagaagat gatcatttgc
     8761 aatcacctct tgcaagtcac gattttcagc aggatagact tgctctatcc ctgttccaat
     8821 gactgcgatt gtccgtccac catgagagat tgctgcttgg tgaccgtatg tatctatccc
     8881 tttcgcgcag ccactgacga tcacatatcc agcttctatc actggtttta gtaaataata
     8941 aactgttttt ttggctaaag cagaagtgtg tcttccacca acaacgctta acatgttaca
     9001 agaaagtaaa gatagatcac cattataaaa cagtacaatt ggcggatagg tcaagtgaag
     9061 cagctgaggg ggatatttgg gatcaaagca agtgataaac gactgtttcg attggatttt
     9121 gccccagtta gcgtgaattt cctgccaact tttgcgaaac agctcccggt gtttggttat
     9181 tttacctatc ttaatgatgt cttcaaatcc gatttcttta taaggatact gcatcatcca
     9241 gttagcaagt gcccattttc cttgactact gattcctttt gtataagcaa gtttcaataa
     9301 attttcttct atttgataca tcataaaacc gcctttcgtc tctctttcta ataagatacg
     9361 taaaagcggc tgttttttat tgtaaatatt tttttacagg tgcaaaactt ttacgatgga
     9421 ttggcgtaat tccttgtcgt tcaagtcctt ctaagtgttc ctttgttcca taacctgcat
     9481 tatgcgagaa accatatcca ggataaagca cgtcatattc tttcatcaat tggtctcgat
     9541 atactttagc aatgatactt gcagcagcaa tcgacacaga tcgtgcatcc cctttgatta
     9601 ttttttcctg ctgaataggc aaatccaagg tcatcgcatc gatcaacaaa tagcttggta
     9661 caatggacaa tttttctact gcttggatca tcgctaattt acttgcttca taaatattca
     9721 catcatcgat tgtctgctga tcaacgatcc cgatcccgat tgccagggct ttttcttgaa
     9781 cttgttgata aagaagttct cgctttttct ctgataattg tttggagtca ttcagcccta
     9841 atatgatctc atttttcggc aagataactg cagctgccac gacaggtcca gctaatggtc
     9901 ctcttcctac ttcatcaatg ccggcaatcc attggtgacc ttgtgcgtac ccttgctttt
     9961 caaagcaaaa catttcttca tatttttcct taattaatgc ttcctttttc aatcgtcgtt
    10021 caaaagaagc cagtgcttgc tgcacgcctt tgcgtggatc atttcttaat gattctagtg
    10081 taggatcatc tattctctgt atgcttttca atcgttcttt gatactttgg atggattcag
    10141 tcatcacttt tttgctccat ttcttcgcaa cgatccagtg tgtatcgtcc aagttttcct
    10201 tgacgaatat catgcacgac taattcgctg ccacgatcat agtcttcttt atacccacgt
    10261 tttgctgtaa tatccatcag taattccggt gccaattttt cttcttgctc gattgataaa
    10321 ccataacgat cgctgattct tcctgaataa tatcgggcaa aaaagtctaa tccgtaaatc
    10381 gctaaatcat ctaaatggac aagctggtct ttgatcgcgc ctgtcagcgc tagttttttg
    10441 ccgatttctt catcttcaaa tttgggccat aaaatccctg gtgtgtctaa caattccaac
    10501 tcattaccta aacgaagcca ctgctgacct tttgttacgc caggcttatt tcctgtctgt
    10561 gcaattttct tgcctactag tcgattcatc aacgtagatt tcccaacatt tggaatacct
    10621 aagaccattg cccgaatagc gcgaggcttg atcccccgag cagcatctct tgcccgtttt
    10681 tctttcaatg cttctttagc cgcaggtact agacctttaa ttcctttact ttcttgtgcc
    10741 gtgatagcta atgcaagatg gccttgttta cgaaaatatt cttgccacag ctttgtctgt
    10801 tgtggatctg ccagatccgc tttattcaat aaaacaaggc gaggtttttg ctgaatgatc
    10861 tgatctagca ttggattacg agaagataaa ggtaatcgtg cgtctaccaa ttcaaatacg
    10921 atgtccacat atttcatttt ttcagaaact tctcgtcggg cttttgccat atgtcccgga
    10981 aaccattgga tggtcattgt tctctccatt cctttcatac cggaatattc cccggcttta
    11041 cttgtttttt tctttttcaa tataaattca cttttttatt ataaagaaat tccatctatt
    11101 agttaagtga aagtgaacca gtcctcattc tatcacatct tcactttttt gatgatgcaa
    11161 atttttattt tggtttctat atatgaatct aaatttttga cagcgtttcc taaataagag
    11221 atgatagacg aacaaaacag aaaggagacg aaacaaatga atagtatggt ttttgttaat
    11281 tttcctgttg ctgatgtcaa tcgttccgtt gctttttatg aaaagctggg ttttaaaaaa
    11341 aatgaagaat tttctactga agaagctggt tcaatggtat gggatgacac tttttggata
    11401 atgcttctct ctcatgattt ttataaaaaa tttataaaag ataaagaaat agctgataat
    11461 aaaaaaacaa gtggtgcttt gatttctttt agtgttgaaa atgcggatga agtaaaaaaa
    11521 tttgctcaaa ttgcaaaaga aaatggtggt actttccacg aagtagatat gggaatgcct
    11581 gaagaagaaa tgttttcatt agaagtccaa gatctagacg gaaatacatt agaacccgta
    11641 tggatgaaga tgtaagcaga taactaatct gacgtttttt ataacaaaaa aacaaaggcg
    11701 gatgatacga taagtattct ccgcctctat tttttttatt cgtacaatct cttttttaca
//
LOCUS       DACGLW010000090        11254 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.094, whole genome shotgun sequence.
ACCESSION   DACGLW010000090 DACGLW010000000
VERSION     DACGLW010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11254
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.094"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            complement(17..1246)
                     /gene="hflX"
                     /locus_tag="IXT53_002426"
     CDS             complement(17..1246)
                     /gene="hflX"
                     /locus_tag="IXT53_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HAQ4906209.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="IXT53_002427"
     CDS             complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="IXT53_002427"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HAQ4906210.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2169..2906)
                     /locus_tag="IXT53_002428"
     CDS             complement(2169..2906)
                     /locus_tag="IXT53_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HAQ4906211.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2997..3524)
                     /locus_tag="IXT53_002429"
     CDS             complement(2997..3524)
                     /locus_tag="IXT53_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HAQ4906212.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3704..4297
                     /gene="clpP"
                     /locus_tag="IXT53_002430"
     CDS             3704..4297
                     /gene="clpP"
                     /locus_tag="IXT53_002430"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HAQ4906213.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4401..4886)
                     /locus_tag="IXT53_002431"
     CDS             complement(4401..4886)
                     /locus_tag="IXT53_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HAQ4906214.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(5039..5236)
                     /locus_tag="IXT53_002432"
     CDS             complement(5039..5236)
                     /locus_tag="IXT53_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906215.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="IXT53_002433"
     CDS             complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="IXT53_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HAQ4906216.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="IXT53_002434"
     CDS             complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="IXT53_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HAQ4906217.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            9211..9423
                     /locus_tag="IXT53_002435"
     CDS             9211..9423
                     /locus_tag="IXT53_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HAQ4906218.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9425..11104
                     /locus_tag="IXT53_002436"
     CDS             9425..11104
                     /locus_tag="IXT53_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HAQ4906219.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
BASE COUNT         3265 a         2429 c         1971 g         3589 t
ORIGIN      
        1 ttaaaaaaat aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc
       61 ctttaacttc atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc
      121 ttctcaactc gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca
      181 ttagttccat cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct
      241 gagtagaaat caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt
      301 ctgccttatt gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt
      361 ttaagacagt ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca
      421 aaagatccat attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag
      481 gcaggtcttg gatgaacccg acggtatccg ttacagtaat ctcaaatcct tcagcaaaac
      541 gccatctctt agtcaatgga tctaacgttg caaacaattg atctttcgaa taagtatccg
      601 cttgtgtcaa tagattcaag atagttgact tcccggcatt cgtgtagcca ataagtccaa
      661 tctgaaaaat ctcgctattt tttctttttt ggcgattgcg ctctcgatga gcttctactt
      721 ctttcaattc tttcttaaca gcaaaaattt tattccgtat atgtcggcgg tctgtttcta
      781 actttgtttc acctggccct cttgttccga tcccaccgcc aagacgagat aatgttttcc
      841 cttgccctac taaacgaggc agcaggtatt ctaactgagc aagttcaacc tgcagcttcc
      901 cttctttcga acgagcacgc atcgcaaaaa tatctaagat aagctgcacg cgatcaatta
      961 ccggaagacc aacagcatcg gtaatcagtt ggctctgtct cggagttagc tcatgattaa
     1021 agatgatcaa atccgcttca tgcgcatccg cttgttgaac taattcttgt aatttccctt
     1081 taccgataat cgtttgccga tccacttgtg gtctcttttg tgtaattgtg aatacgactt
     1141 ctcctgatgc ggtattcgtc agctgaacta gctcttccat tgactctgaa aagtaacgct
     1201 ggtttgcttc ggtttctacc cctacgatga tcactttttc ttccatttta ttccgcctcc
     1261 agccattttt tgattgtttg ctcaatctga cttttttgat ttggctcact tataagatca
     1321 aaccactgtg catccaaacg gttacgaaac cacgtcaact gacgtttcgc gtatcttctc
     1381 gaatgtagtt tgacttgttc tgttgcttct tccaatgttt ctttcccttc aaaataaggg
     1441 aaaaattctt tatatccaat accttgactg gcctggactt ctccaagctc atacacctta
     1501 cgcgcttctg ctactaaacc ttctgctagc atcaaatcga ctctttgatt gattcgttca
     1561 taaagaatac tacgcttcgt atctaatcca atcatgaggt aatcatacaa ttgctttggc
     1621 tcttctttag gtgccgtgat actatgtcct gtcgtctcta aaacttccag agctcttatc
     1681 actttgcgtt gattgttcca gtggattttc tcagcagctg ccgggtcttt ttctttcaac
     1741 tgattccata atgcttggga accaattgtt tcagctaacg tttcatactt ttttctaatc
     1801 tcaggattcg cttcttctgt cgctcctaac tgataatcat atagaaggga ttggatatag
     1861 agacccgtac caccagctat gataggtagg tgccctcggt tcgcaatctc ggtaatcaat
     1921 tgtcgacctg aatgctgaaa atcagcggct gagtaacgtt gagtaggttc gatcacatca
     1981 atcaaatgat gcggaacatt attcatctct tcttctttga tttttgctgt accgatatcc
     2041 agatttttat agatttgcaa tgaatcacca ctgatgatct ctcctccaaa tagttgtgcg
     2101 agttccacac ttagcgctgt tttaccgact gctgtcggac cgacgatcac taatactttt
     2161 tccatatttt acctactttt cgttgattgt ttccaccgaa gagctttctc cggaaaatca
     2221 gttattattc catctatttt gagttcttga catttggcca tttcttcttc atcgttaatc
     2281 gtccatgcac gaattgcttt tggaaaatca gctgcctgga ctggatgttc gaacaaccaa
     2341 gtattttttg gatgtactcc ttctatataa ggggtttctg ctgctagttg gatcttttta
     2401 tcagaagtac tcatcaataa gtccagctgt atctccggtt ccagtaatgc gattttttcc
     2461 aaagaatcca aatgaaagct gcaatatgca tgttgaaaag gccaatctct actttttaat
     2521 gtttcggcta atttttcttc gataccggga tattcatact gatcagtttt aatttcaatc
     2581 attagcatcc ctcggtaatt tttttggagg agcagtccta acacttctct caattcagga
     2641 actttagctg ctggaaattc tttgctgaat ttgccccctg cattcaattt tttgagttct
     2701 tccaatgttt tttctcgaat cagcccttta ccgttagtcg tccggtccac ttcttcgtcg
     2761 tgcataacga tcaaataacc atctttgctt agatggacat ctagttcgat accatctgca
     2821 cctactcgta cagcttctgc aaatgcagga agcgtatttt ctgggtgggt gccgctgctt
     2881 ccgcgatggg catagatttg taacatcttt cttcactccg ttcagttcta ttgtatcatt
     2941 aagaaaaaaa gaaagcatta ccacgctctc atgtgcaacg gcaatgcttc aaaatatcag
     3001 ctccaaacat catctttcga gaagaaacca atcggctggc gcgtcatagt cttcaactga
     3061 gctttttttt cattgatcat ttcagtcaga ctatccgtaa aattctctaa aatcacgcgt
     3121 ccgcctttat aagcatatcc accaatcttc attgtttcat ctggagataa aatcactttt
     3181 cgatgggaga tattcttaaa tagctcgtct aaaatataat ttggcagata aaaactattt
     3241 tgcgctgaaa atgattggag atacgcaaaa tcgtcaatcg tgatataaca atcatcaaat
     3301 gcatcaaagc tgacatattc ttccatcagc gttgtccgtt tttgcaagat ctcgttgatc
     3361 tttttattta attgattcag cgtctcgatt tctttgactt tcaattccaa ttcacgtcta
     3421 cgctgttctt tattttccaa gtgacgcatt tgcagtatca tgccgatata gacaactaca
     3481 gcacctgtca gcactccgat aaggccagta ataccaacta tcaatctgtt gttcctcctt
     3541 gttttgtttc tctgcttatc ataccataaa gcgggtttat gcgactaatt tgcctaagtt
     3601 ttttatttga ccttttttga ccaaaaatgt atactacatt ttaggtatta gaaactaaat
     3661 tatggtacac tatttctaaa cgaaatcaag gaggaactct attatgaatt taattcctac
     3721 agtcattgaa caatcttcac gaggtgaaag agcttacgat atctactcac gtttattgaa
     3781 agaccgtatc atcatgttaa gcggccaagt aacagatgat cttgctaatt cgatcatcgc
     3841 ccaattgctt ttcttagacg ctcaagattc tgaaaaagat atctatcttt atatcaattc
     3901 tcctggtggt tcagttacag caggtatggc tatttacgac acaatgaact ttgtcaaagc
     3961 agatgtccaa acaattgtta tgggtatggc tgcttcaatg ggaagcttct tgctgacagc
     4021 tggtacaaaa ggtaaacgat ttgctttacc aaatgctgag atcatgatcc atcaacctct
     4081 tggcggcgct caagggcaag caactgaaat cgaaattgcg gcacgccata ttttgcaaac
     4141 acgcgaacgc ttaaacaaaa ttttagctga acgcactgga caaccgcttg aagtgattga
     4201 aaaagataca gatcgtgaca actatatgac agctgaacaa gcaaaagcat atggattgat
     4261 cgatgaagtc atggaaaaca gttcttcatt aaattaatct gtttcaaagg aagctttttt
     4321 aagctctctt atctaaacca aataaaaaag gatggtcaac tcaaactgac catccttttt
     4381 tattttatgg atgctactgc ctatctttgc tccttgaatt ttccttgcgc atgcatccga
     4441 cctatctcga cataatgctg gatgttttct ttgttttttt gtatttcttc ttttgtcaaa
     4501 ggacggatca ctttagctgg gcgaccaaaa gccaagacat taggaggaat aacttttcct
     4561 tctgttacta atgatccagc accaatcaaa ctattctcac caataaccgc atgattcaat
     4621 atcgtggtcc ccatcccgat cagcgcccct ttttcgattg tacagccatg tagcatgcac
     4681 tgatgtccta ccgtcacatt ctccgcaata tctacaggtg catcatgatc gacatggatg
     4741 attgtaccat cttggatatt cgttctttgt cctattttta tccaattact gtccccgcgt
     4801 aaaacagctt gataccagat cgtcacatct tctgacaatg tcacatcacc aatcactgtg
     4861 gcatttgatg ctataaaccg ttccatattt atttttctcc tttcattttt tgtagaaact
     4921 aatcctattg aataaaggta tcatagcata tatttatttc ataagaaaaa aaacaggtaa
     4981 aacctgctca ggccagatgt ctatttacag tatctggtaa ctgacgtttt acctgttttt
     5041 attgtacatc tatattttct tctattactt tttttaaaat taattcgtac acttctattt
     5101 gtgctgccaa gcgttcgatt tgaaactcat tttcaaaaaa atcagctgaa tcaaaaggct
     5161 tcgtctgaca gacgtgcgtt aaacgatcaa cttctctttt ggcttcagta attttttcat
     5221 ttaaaaactg ttccatctcc gtgtcctcac ttttcttgat aactttactt taccattatc
     5281 aagaatagag aacaaaaaat aagttttaaa ctgttttatt cttatatgac ttattctgct
     5341 tcctcagcaa attggctgtt atacagtttt tcataaaatc caccttgtgc caataaactc
     5401 tcatgcgttc ctttttcaat gatctgacct tgatccatca ctagaatcag atctgcatca
     5461 cggattgtcg ataaacggtg agcgatgacg aaacttgtcc gtccctccat cacgcgatcc
     5521 attgcttttt gaatcaacgc ttctaaacgg gtgtctactg aacttgttgc ttcatctaaa
     5581 atcaagattt taggatcgga gaccactgct cgggcaatcg tcagcaactg tttttgtcct
     5641 aaagacacat tgtctccctc agaattgatg gccatatcat atccatctgg catcgttcgg
     5701 ataaaatgat ctacgtttgc tgttttcgca gcatcaacta cttcataatc tgtcgcatca
     5761 agctttccga agcggatatt atctgcaatc gttccttgat aaagccatgc atcttgcaag
     5821 accataccaa atagcgaacg cacatcactg cgactcattg atttggtgtc gattccatca
     5881 atccttattg ctcctttgtc tacgtcataa aaacgcatca atagattgat caatgtcgtc
     5941 ttgccggcac cggtcggacc gaccacagct acggtttgac cagctttcac ttcaaaattc
     6001 aaatcttgga ttaatggttt cttcggatca tatgcaaaat cgacatgatc gaatgtcaca
     6061 tctcctaaaa tcgtttctgg cagtgaagca tctactttgt tttctttttc ttctggctca
     6121 tctaaaatct cgaatacacg tttcgttgcc gctgatgcac tttgcaaaac tgaagacaat
     6181 tgtgtcacgt tccccattgg ctgactgatt tgccaaatat attggatgaa tgcttgtagt
     6241 tgaccgacaa cgatcgcccc agtaataaca taaaagcttc ctagaacagc cataccaata
     6301 taagtagcat aggcagttaa ttgaactaga ggcatcatca agccagaaat gaatgctgca
     6361 cgaaacccaa agtatttcaa gctgtgatta acttttttga atccttccaa tgtttctttt
     6421 tcgcgtccat atactttcaa tacactaaaa cctgtcatat tttcttggac gtagccgttt
     6481 agctcaccaa gagaattctg catgctttga aaatatttct gcgaagcatt gatcactcgt
     6541 tttgagatcc agatcgatgt tggaatcatg atcatagaga tcagcgccat catcggctgg
     6601 atataaaaca tcattgctgc agccaacgta attcccaata tcgcatttac gataccaata
     6661 aaagcttgtt gtaaagcacc gctgactgca tctacatcat tcgtcacacg agacaaaata
     6721 tttccttgct ggtttttatc gaaatatgaa accggaagac gattgatttt ttcttctatg
     6781 tctcttctca aatcatgcat ggcactttga acgacatttg tcatcaaaac accagaaagt
     6841 aattgcgaaa tacaataccc tattccaaca actgtgatcc aaatgagaca tttgaatata
     6901 taagaaaaat tgattgattc gccattggca atattacggc tgatttctgt agtaggaagt
     6961 cctgtaacat atggcaaagc cacgttgaaa gccactgtca gaactgtaaa taggatcact
     7021 aaaagaaaag tctcacgata cggtctgata tatcgagcta aacgttttaa ggaagaaaag
     7081 gtttttttca tgctaattcc tcctttgata attgagaagc ggcaatatca taataaatcg
     7141 ggcatttttc caacaactcc cgatgcgtcc ccataccgac gacttctcct tcatttaata
     7201 cgatgatctt atctgcatgc atgatcgttc ccacacgttg tgctacgatc aatacagtag
     7261 attctgtcgt ttcttttttc agacgagcac gtaacttcgc atccgtttga taatctaatg
     7321 cagaaaagct gtcatcaaaa atatagattt ccggacggcg tatgatggct ctggctattg
     7381 ccaatcgttg tttttgtcca cctgaaaaat tggaaccacc ttctgataaa tgttcattaa
     7441 atccatctgg ttttcgagaa atgaattctg aagcttgagc aatctcagct gcacgttcca
     7501 tttcttcttg cgtagcatct tctttcccgt atcgcaaatt atctgcaatc gttccagtaa
     7561 atagcagcgc tttttgcgga ataaacccaa tcttatctcg caacttgctt agccgataat
     7621 ctctcacgtc tactccatcg atcaagatct ttccttcact cacatcataa aagcgaggga
     7681 tcaattggat caacgtagat tttccgctac cggtacttcc aataaaagca acggtctctc
     7741 ctggagaggc tgtaaaactg acatttcgta tcactggctc ttgcgaatgg cctggataag
     7801 caaatgttac attttgaaat tcgatatacc ctttagtttt tgtatcagat ataccatttg
     7861 gatcttctac aatcgaaggt ttggcatcta gggcttcttg gatacgtcca gctgaaacag
     7921 ctgctcttgg atacatcatg aacacactag caaataacat gaatgagaac aaggcatgga
     7981 atatatactc gataaacgca atcaaatctc ctacacgaag ctcgcctgca tcgatttgca
     8041 ctgccccgct ccagatgatc aataccatta cgatattaaa taagaaataa aaacccggtt
     8101 gtgcaacagc catcaaacgg aataagcttt ttgaactaga agcataattc tcattgacta
     8161 cagcaaaacg tttttcttca aacttttcat tgacgaatgc acgaatcaca cgcaatcctg
     8221 aaagattttc ccgcaagata ctgttgattt tatctaagtt tttttgttgc tttttagata
     8281 acggttcaga caccttagca atcgctacga ctgccaataa tagtaaaggt aatgcaccta
     8341 ctacatacaa actgagtgtc acacttgtac gcatgaccat gtacaaactg acaatgaaca
     8401 tcattggtgc cataaaaccg atacgtaaaa tattttgcag gaacaacatg atctgataag
     8461 catcgtttgt tgtacgagtg atcaatgaag atacgcctag attttcgtat tcctgatgcg
     8521 aataactttg gatcttttcg aacaaatcat ctcgaatatc tgctacgata ttagtcgtaa
     8581 tcctcgaacc aaaataacct aacaagatat tcaaaataat accgattaca gtaataacga
     8641 tcataaatat cccttgttgc ttcacataat caaaatcatc attgcggatt cctacatcga
     8701 tcatacgtgc taaaatcgta ggaagtccta attcaatcaa aataaaacca aatacgcata
     8761 tgaaattgaa aaacaagaat ttcttataac gcatggtata atgccacatt agtttcactt
     8821 tctcatctcc ttttaatctt ttgttacatt ttcattacta aaaatgatag cacaacgagc
     8881 ctattaatag aacacttctc tcataaaaaa gtcattatga ttatagcggg atgataaaca
     8941 ttgatttttt ctttcaatct agttgatttc tatacttatt tgctgtttta tgctagaata
     9001 acaattaatt gttgtatatt ttttatacag aaggtacggt tgaaaaaata tccgaacaga
     9061 taagcaaaaa cgaggcttgt ctctttgact cgaatccagt attctagtca aagcatatga
     9121 aaaaccaggt ggcttgtccc ctcgttttgt gttgtctaaa aaaatcaact agctaaatca
     9181 ctgctttacg cagataaagg agacaaaaca atgatctata aagtttatta tcaagaaaca
     9241 aaagtacgca accctaaacg ggaagaaaca aaatctcttt acattgaggc aaaaagcgat
     9301 gtagacgctc gccaacgtgt ggaagaaaat accccataca atattgaatt cgttcaactt
     9361 ctagaaggaa gtcatctaga gtacgaaaaa gaaaatgctg atttctcatt agtggagttt
     9421 taatatgaaa ctttccatca aaaataacga gacaggcgtc tttgctattg gtggattagg
     9481 tgaaatcggg aaaaatacgt atggggtaca attccaagat gaaatcattt tgattgatgc
     9541 cgggatcaaa ttcccggaag acgatttact tggaattgac tatgtcattc ccgactacag
     9601 ctacatcata caaaacttac acaaagtaaa agccttggtg attactcacg ggcacgaaga
     9661 ccacatcggc ggaataccct acttgctgaa acaagcaaat attcctatct atgctggtcc
     9721 tctcgcatta gcgttgatca caaacaaatt agatgagcat ggcttgcttc gagatgcaga
     9781 gctccatgag atcaatgaag atacgatcat ccgttttaga aagacttctg tcagcttttt
     9841 cagaacgacc catagtatcc cagatgcatt agggatcgtg gtgaaaacac cttctggaaa
     9901 tatcgtagcg actggagact tcaaattcga tttcactcct gttggcgaac cagcaaactt
     9961 gcataaaatg gctcgtatcg gggaagaagg agtcttgtgc cttttatctg atagtacaaa
    10021 tgctgagatt ccaacattta ctaaatcaga aaaaacgatt gggcattcta ttttgaagat
    10081 cttttcaaaa atcgaaggac gtatcatttt tgccagcttt gcttctaaca tcttccgttt
    10141 gcagcaagca gcagatgccg ctgtggcaac tggtcgtaaa atcgctgttt tcggacgaag
    10201 catggaaaat gccatcgtca acggtcaacg tttaggatat atcaaagttc cagacggcac
    10261 atttgtagat gcacacgaaa tcaatcgctt gcctgccaat gaaacgatga tcctatgtac
    10321 aggttctcaa ggagaagcga tggccgcgtt gagccgcatc gcgaatggta ctcaccgtca
    10381 aatccaaatc cagccagacg atacagtgat cttttcaagt tcacctattc ctggtaatac
    10441 aacaagtgtc aatcatttga tcaatttgct gtctgaagct ggtgcagaag tgattcacgg
    10501 aaaaatcaat aacatccata cttccggtca cggtggacaa gaagaacaaa aattaatgct
    10561 tcgtttgatg aagccaaaat attttattcc tgtccacgga gaattccgca tgttgaaagt
    10621 acatgcttct ctagcacaag atgtaggtgt tcctgcagaa aactgcttca tcatggaaaa
    10681 tggcgatgtt ttagcgttaa ctagcgattc tgcgcgtcct gctgggcatt ttgtggctgg
    10741 agatgtgtat atcgatggta gcgggatcgg tgatattggt aatgtcgttt tacgtgaccg
    10801 tcacctgctt tcagaagaag gtcttgtatt agctgtcgcg acagtggata tcaagaaaaa
    10861 agaaatcttg gctggtccag atattctctc tcgtggattt gtctacatgc gagaatcagg
    10921 agaaatgatc aatgaagctc agcgaatctt attccacgct ttaagagaat gtttgaatgg
    10981 acctgactgt tcagaatata aattgaaaga agcgatgact ggcgcgcttc agcctttctt
    11041 attcgaacgt acagaaagac atccaatgat tttgccaatg gtaatgacac cagatgaaga
    11101 ataaataaga ggttatgaaa tcagcacctt gtcctgaata taagagaaaa caaatgaaaa
    11161 acatgagaag ccatttttgt ttgttcttat tccttgatac aaggtggctc tttcatagcc
    11221 tcttaaattt atttactttt tcatacaaaa aaat
//
LOCUS       DACGLW010000091        11110 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.096, whole genome shotgun sequence.
ACCESSION   DACGLW010000091 DACGLW010000000
VERSION     DACGLW010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11110
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.096"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            <1..619
                     /locus_tag="IXT53_002437"
     CDS             <1..619
                     /locus_tag="IXT53_002437"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ4906220.1"
                     /translation="VDIIGYDTYDKINHAYAFGGVAMTINTIQAYLDIPIDFFVTLNM
                     QGLEQIVDSIGGITLTPTATFTQDSYSFYQGEIINLDGKASLAYSRNRHADDSGDYGR
                     QARQREIIMAIIEKGTKVTTLATKIPSLIKSLQGNMTTNLSVDDMWTIINKYSTALGN
                     IEQVQLKGEGQLLDDGIYYEMINEESLEEVQNILQKQLDYHLNID"
     gene            669..1193
                     /locus_tag="IXT53_002438"
     CDS             669..1193
                     /locus_tag="IXT53_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007722706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ4906221.1"
                     /translation="MQNDIRGKIKKSRHIKGISQSEVAKYLNISRQSISKWENDTSSP
                     DINNLTLLSSLYEISIDDLLKEKKEVKKNIKENSIEIIESKNKLIYLKNNTNRDEGLV
                     LLLISLIGCLISPLGLIIAPVVLKRNKATNTFYKGVIAASVICFTVSLFVSYGHFSNI
                     FHWGGSTNVEYIEN"
     gene            complement(1190..1654)
                     /locus_tag="IXT53_002439"
     CDS             complement(1190..1654)
                     /locus_tag="IXT53_002439"
                     /inference="COORDINATES: protein motif:HMM:NF037267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906222.1"
                     /translation="MRQVLLVVTLCLSFFLFEVNTVEAYVANNGVYYNLIQGGTQEFM
                     EVDEEGNPILIRVEEVPGLSLFSVNNGNYKISGSKFGMWKANYYVTVSNNQFIRAYSP
                     SAVAVTGSFTSTRLSLVSSKKASYTLEWKAGILSSNSYLDATLTDNSINITY"
     gene            2568..4109
                     /locus_tag="IXT53_002440"
     CDS             2568..4109
                     /locus_tag="IXT53_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010632830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HAQ4906223.1"
                     /translation="MFRYRRILEMHSEGISIRSIAASTGNSRQKITEVIRKAEEKGIA
                     RALSEEMTDQWLGEFLFPEKQLEANGRHLPDFDKIHKELAKPNVTLTLLHQEYAVECR
                     MSGKIPYAYRTFCDYYNAFARKHKATMRIRRKPGEIMEVDWAGSPAYVIDRDTGEKVK
                     AYVFVATLPCSQLSYAEAFLTMKSVAWITAHNHAFEYFGGSTDIIVPDNLKTGVKKNE
                     GIDVILNESYRELGEHYGSIILPARVSTPKDKPSVEGSVGVISTWIIAALRNTHCFTI
                     EDLNREIRMKLNEFNERPFTKKPGSRIRAFEEEEKFALNPLPLAPYKMSVWKKAKVQL
                     NYHVSVESMFYSVPYEYIQQNIDIRMTENLIELYFNSMRIATHKRLYGKFGQYSTVRD
                     HMPDNHKMYVDHTPESSMEWAETIGSSTVKVVQHLLDRYQNEKQALKSIMSLKSFGKK
                     YSKNEIETACSTALNLASLPSVKIIQTILKGNKQKRATSQSHIEDTNIEKHGFTRGAS
                     YFGGK"
     gene            4109..4861
                     /locus_tag="IXT53_002441"
     CDS             4109..4861
                     /locus_tag="IXT53_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ4906224.1"
                     /translation="MMLNEQTEVKLAELKLSGMLESYREQMTNKEYQSMSFEDRFNLM
                     VDLEHSRRKNNKLQRLIKSANFRNASASIEDIEYHPDRNLDKNLILKLASGTYLEEHH
                     NIILMGASGNGKTYIANAFGIQACRQYYNVKYIRLPELLDELTISKNQADGSFRKIIN
                     RYKKVQLLIIDEWLLTNLSLENSTFLLEIIESRLKTASTIFCSQFHPEGWHEKLGNPQ
                     IADAILDRIIHDSYQILVDGKVSMRERYGIRN"
     gene            complement(5044..5619)
                     /locus_tag="IXT53_002442"
     CDS             complement(5044..5619)
                     /locus_tag="IXT53_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906225.1"
                     /translation="MKNKFKYLFTILALLPITTSTLITPTIAKASEKQEDSVSYEIVK
                     NDLYIQFEEELAELIASNQSTVSVPDLFSTQSVLSTSSQDNSISEEQFFSLVEKYDGA
                     ELTAVVNSQDAFTTKFVNGGVNSYATSYRGLNALRSELKYNAGALKVGGTLVGTAGGL
                     LGVLLGMVTGDYLSGHCDDAAAGVSAFIQAG"
     gene            6407..6979
                     /locus_tag="IXT53_002443"
     CDS             6407..6979
                     /locus_tag="IXT53_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HAQ4906226.1"
                     /translation="MNKAIFWDLLGTLGGDSRTLINQNFNFFKDTIPALKKAAQSHYL
                     NIIITNQSHISHGRITMAEYTDALESLINELEKNDIQITDVYTCPHARKDNCDCKKPQ
                     PTFVNEALKKYDLNPSKCFVVGDSGKNDMMLAKNAGINSVLLLTGEGIDSLTKYRHLW
                     SETKPTFIAENSLDAINKIHMYADGDSSDS"
     gene            7172..7735
                     /locus_tag="IXT53_002444"
     CDS             7172..7735
                     /locus_tag="IXT53_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ4906227.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAV
                     RPQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQ
                     VYSVKEITEMTGISKATLYRYLKDNRE"
     gene            complement(8014..8301)
                     /locus_tag="IXT53_002445"
     CDS             complement(8014..8301)
                     /locus_tag="IXT53_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ4906228.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPILLCGTHENFYEQLKKIR"
     gene            complement(8291..8620)
                     /locus_tag="IXT53_002446"
     CDS             complement(8291..8620)
                     /locus_tag="IXT53_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007723369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ4906229.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            8925..9254
                     /locus_tag="IXT53_002447"
     CDS             8925..9254
                     /locus_tag="IXT53_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ4906230.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            complement(9541..10026)
                     /gene="vanZ"
                     /locus_tag="IXT53_002448"
     CDS             complement(9541..10026)
                     /gene="vanZ"
                     /locus_tag="IXT53_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HAQ4906231.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(10179..11090)
                     /locus_tag="IXT53_002449"
     CDS             complement(10179..11090)
                     /locus_tag="IXT53_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HAQ4906232.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
BASE COUNT         3778 a         1945 c         1848 g         3539 t
ORIGIN      
        1 tgtcgatatt atcggttatg atacttacga caaaattaac catgcctatg cctttggtgg
       61 agtagccatg actataaata caatacaagc atacttagat atacctattg acttttttgt
      121 aacgttaaat atgcagggtc tagaacaaat tgttgattct attggaggaa taactcttac
      181 tccaactgcc acttttacac aagatagtta ttcattttat caaggcgaaa ttattaactt
      241 agatgggaag gcttccttag cgtactctcg aaatcgtcat gcagatgatt caggtgacta
      301 tgggagacaa gctcgtcaaa gggaaataat aatggcaatt atagagaaag gaactaaagt
      361 tactacacta gcaactaaga taccttcact cattaagagt cttcagggaa atatgactac
      421 taatctgtca gttgacgata tgtggactat tattaataaa tattccactg ctttaggaaa
      481 tatagagcaa gttcaattaa agggggaagg acaactactt gatgacggaa tatactatga
      541 aatgattaac gaagaatctt tagaagaagt tcagaatatc cttcaaaaac aactagatta
      601 tcacttgaat atagactaaa taatctattt cttaatatcc aataaataaa agtcattaat
      661 aggagaggat acagaatgac attaggggaa aaattaaaaa atcacggcat ataaaaggca
      721 tctctcaaag tgaagtagca aaatatttaa atatatctag acaatctatt tctaaatggg
      781 aaaatgatac tagttcccca gatataaaca acttaacttt attaagtagt ttatatgaaa
      841 tctctatcga tgatctttta aaagaaaaaa aagaagttaa aaaaaatata aaagagaata
      901 gtatagagat aatagaatct aaaaataaat taatttattt aaaaaataat acaaaccgag
      961 atgaagggtt agttttattg ctcatttcct taatcggctg tttgatttct cctcttggac
     1021 taattattgc gcctgttgtt ctgaaaagaa ataaagcaac aaacactttt tataaaggag
     1081 tgattgctgc ttcagtcatc tgttttacag taagcctgtt tgtctcctat ggacatttta
     1141 gtaatatctt tcattggggc ggctccacta acgtggaata tattgagaac taatacgtaa
     1201 tattaatgga attatctgtc agtgtagcat ctagataaga gttactagat aaaattccag
     1261 ctttccattc taatgtgtag ctagcttttt tgctggagac gagagacagt ctcgtgctag
     1321 taaaggatcc ggtcactgcg actgcagaag gggagtaggc cctaataaat tggttattcg
     1381 agactgtcac atagtaattt gccttccaca ttccaaattt agatccagat attttataat
     1441 ttccgttgtt gacggagaac agggagagcc cgggaacctc ttccacacga atcaagatag
     1501 gatttccttc ttcgtctacc tccatgaact cttgcgtgcc accttggatt aagttatagt
     1561 agactccatt atttgccaca tatgcttcta cagtgtttac ttcaaataaa aagaaagata
     1621 aacataaagt aacaaccaat aaaacttgtc tcaaaaaatc cctcctaaaa ttaatatact
     1681 catagtttaa tatatacggg tttaattagt aagagaacat tttgtgaaca ttcttatatt
     1741 gaaacggctc tctctacaaa tggacgcata tataagtgta tctgggttag taaacattca
     1801 ctgttaccaa ctctacttac taaacagata aaatatcaac gaaataaaaa aaccaaatca
     1861 actaattttt tactaataac gtagataata aaatcaaaaa gcaaatttga gcggttgcaa
     1921 ctataaagtg acctgatgta aaccataaga tgcttaatta agctatactt agatgagaca
     1981 ctatacttag gaagaactta gattagcata gcttcctaga acaaaaaaat acgagaggaa
     2041 gattctttta tgactaataa atatctatct gtatttactt ttttggttgg cttgtttttt
     2101 gttattaata gtgtagcaag ttctgatttt gatcttagta agattactct tttaactgta
     2161 ctaccaataa taataggtag tgtgttaatt cttttttctg tgacttccct ttttagacag
     2221 cgaggtaatt ccaaaaagca ttagcgaaaa agataaaagc agttttctct ttacccaaga
     2281 gagaactgct ttttatcatc tccacatttc ttgcttaatt aattacagct tcataaatac
     2341 tatcaatatg gctaataggg aaaccttcag taagggttac cctagatcct cctctagtgt
     2401 aaccagtgat tacggcatcg aaaatgtacc atctctgcaa taatttttac caggctcggc
     2461 aaagaaatgt accagtaagt gcaaagcagt gtaccacaaa tacctcttgt ataatgtagg
     2521 tgcacacctt agggtgtgac atttatttta caggaggtat ttttattatg tttcgttatc
     2581 gaaggatact tgaaatgcac agtgaaggaa ttagtattcg cagtattgca gcaagcacgg
     2641 ggaactcacg tcagaaaatt actgaggtta tccgaaaagc tgaggagaaa gggatagcga
     2701 gggcgttgtc ggaagaaatg accgatcaat ggctaggcga atttttgttt ccagaaaaac
     2761 aattagaagc caatggacgg cacctgcctg acttcgacaa gattcacaag gaattggcca
     2821 aaccaaacgt gaccttgacg cttcttcatc aggaatacgc agttgagtgt cgcatgagtg
     2881 ggaaaatccc ttacgcctat cgtacatttt gtgactatta caatgcgttc gcgcgaaagc
     2941 ataaagccac tatgcgcatc cgtcggaagc ctggtgaaat tatggaagtg gattgggctg
     3001 gatcgccagc ttatgtcatc gatcgagata cgggtgaaaa agtaaaggct tatgtctttg
     3061 tggcaacctt accgtgcagc caattgtctt atgcggaagc attcctaacg atgaaatcgg
     3121 tggcttggat tacggcacat aaccatgcat ttgaatattt tggcggttct accgacataa
     3181 ttgttcccga taatctaaaa acaggtgtaa aaaagaatga aggcatcgat gtcatcctta
     3241 atgaatcata tcgagagcta ggggaacatt acgggtccat cattttacct gcccgagttt
     3301 ctactcccaa ggataagccc agtgttgaag gcagcgtagg cgtcatctcc acttggatta
     3361 tagctgcctt acgcaatacg cattgtttta caatcgaaga tttaaatcgt gaaatcagaa
     3421 tgaaattaaa tgaattcaat gagcgaccgt tcactaagaa accaggatcc aggatcagag
     3481 ccttcgagga agaagaaaaa ttcgcactta atcctcttcc actggcacct tataagatgt
     3541 ctgtctggaa aaaagcaaag gttcaactaa actaccacgt ctcagttgaa agcatgtttt
     3601 attccgttcc atacgaatac attcaacaaa atatagatat tcgaatgaca gagaatctaa
     3661 ttgaactata ttttaattcc atgcgaatcg caacacataa acgtctgtac gggaaatttg
     3721 ggcagtattc gactgtcaga gatcatatgc cagataatca caaaatgtat gtggaccata
     3781 ccccagaatc ctcgatggaa tgggctgaaa ccatcggttc ctcaacagta aaggttgttc
     3841 aacacctctt agaccgttat caaaatgaaa aacaagcctt aaagtcgatc atgtctttga
     3901 agagctttgg taaaaagtat tctaaaaatg aaatagaaac ggcctgttca acagctttaa
     3961 atctggcttc tttgccttct gttaaaatta tccagaccat tctaaagggc aacaagcaga
     4021 agcgagcaac aagccaatca catattgaag acactaacat tgaaaagcat ggattcactc
     4081 gcggtgcgtc ctattttgga gggaaataat gatgttaaat gagcaaacag aagtaaaatt
     4141 agctgaattg aaactttcag gcatgcttga atcctatcgt gaacagatga caaataaaga
     4201 gtaccaatcc atgagtttcg aagaccgctt caacctcatg gttgatctgg agcattcccg
     4261 gcgaaaaaat aataaactac agcggttgat taaatctgca aactttagaa atgcctctgc
     4321 cagcattgag gatatcgaat accatccgga ccgtaattta gataagaacc tcattttaaa
     4381 actagccagt gggacttatt tggaagaaca ccataacatc attttgatgg gcgcttcagg
     4441 gaatgggaaa acgtatatcg ccaatgcctt cggaatacag gcatgccggc aatactacaa
     4501 cgtgaaatac atccgactcc ctgaactact agatgaatta accatctcga aaaatcaagc
     4561 agatggtagt tttcgaaaaa taatcaacag atataaaaaa gttcaattac tcatcatcga
     4621 tgaatggcta ttaaccaatc tttcgttaga aaattccacc tttctattgg aaataatcga
     4681 atcccgtctt aaaacagcat caactatttt ctgttctcaa ttccatccag aaggatggca
     4741 cgaaaagctc ggcaaccccc aaatcgctga tgccattcta gataggatca tacatgattc
     4801 ttatcaaatt ctggtagacg gtaaagtttc tatgcgtgaa cgttacggca tccgtaatta
     4861 aatgagtcat taaaataaat taagagccac tccatgattt tcatgagtgg ctcttagttg
     4921 accttgtggt cattattgtc gcttttatta tttgaaatgg taaccgcgtc gcacttactg
     4981 gtacatttct ttgcacttag tggtaaaaaa caatgctcta gtggtacatt ttcgatgccg
     5041 tattcaacca gcttgaataa aggcactaac acctgctgct gcatcatcgc aatggccaga
     5101 taaataatcc cctgtaacca tgcctaataa tactcctaac aatcccccag ctgtgccaac
     5161 taaggtacct cctactttca aagcaccagc attatacttc aactcgctac gcaatgcatt
     5221 taatcctctg taagaagtag catatgagtt aaccccacca ttcacaaatt tagttgtgaa
     5281 agcatcttgt gaattaacaa cagctgtcaa ttcagctcca tcatattttt cgactaaaga
     5341 aaaaaattgc tcttcactaa tagaattatc ttgactagaa gtagataata ctgattgtgt
     5401 agaaaataaa tcagggactg aaacagttga ttgattggaa gcaatcaatt cagctaactc
     5461 ttcttcaaac tgaatgtaaa gatcattttt tacaatttca taactaacag aatcttcctg
     5521 tttttcagaa gctttggcta ttgtaggagt aattaaggtc gatgtagtaa ttggcaataa
     5581 ggctaaaata gtaaataaat acttaaattt atttttcatt ttttatcctt ctttctattt
     5641 tttctacttt taataccaca acttgaaaaa taaaaaatct aagcaagagt aacatctctt
     5701 acgtaatgat tttgcaatag tttttcctcc tctcacaatc tattattatg tttttcgagc
     5761 tgtttcaaaa gcacttttct atttacatct gggaaactaa tcgttgcttt tataaaaaaa
     5821 gatgatagca gaaaaaattg aacttttctt tactataagg tatttataaa atcatcttca
     5881 taggtcaaag tagtagtatt aatctcaact ttgataagct ggtgtatatc aattttctct
     5941 ttaatatgta caatcattaa aaatagattt ttttactatt tatggtttca attaacaaaa
     6001 gaacctccac actaaatcac tataaattta ttatcacctt agttttttat gtgctcctag
     6061 catggaaatc ccatcaaaaa agtaaggttt gtattcaaat gtttttcgcc aacgagggta
     6121 tcttcttttt tacactcttt gctgtatcaa aatataactg aaattctaag tctcttctat
     6181 atcagtgatt atgtgattcg aaatatccat tccctcaaaa gaagttcata tagaaagtga
     6241 atagagagtg gctcctgtaa atatgatata gttaaactgg caaaataaaa tatgcttttt
     6301 caaaaagtaa tatttttgtt ttaattacga agaactactt ttaaatactg taagcacctt
     6361 ggcttgttag ttaactattc ttaaaatgag agggagacaa atatccatga ataaagctat
     6421 attttgggat ctgttaggta cgctgggagg ggatagtaga acactgatta atcaaaattt
     6481 caattttttt aaagacacaa taccggcctt aaaaaaagcg gcacaaagtc attatctaaa
     6541 tatcatcatt acgaatcaaa gtcatatttc acatggacgt attactatgg cagaatatac
     6601 tgatgcctta gagagcttaa taaatgagtt agagaaaaat gacattcaaa taacggatgt
     6661 atacacttgc cctcatgcta gaaaagacaa ttgtgattgc aagaagcctc aacctacatt
     6721 tgtcaatgaa gcattaaaaa aatacgattt aaatccaagt aagtgtttcg tagtaggcga
     6781 ttccggtaaa aacgatatga tgttggccaa aaatgctggt ataaacagtg ttttactttt
     6841 aacgggagag ggaattgata gtttaaccaa atatagacat ctgtggagtg aaacaaaacc
     6901 tacttttatt gctgaaaaca gtttagatgc tataaataaa atacatatgt atgcagatgg
     6961 agactcttct gattcatgat accagaagag tctccattta ttactaaaaa ccgtatcaaa
     7021 aacaatcgat tatgagacaa aatgaaatga cctcttttat taaataaaag aggtcatatt
     7081 ttgtatcata acttgttgca ttaactatgt atctataact cccttttatg atacaatcaa
     7141 gccagaatta aagtacgaaa ggaagaaaag catgagtgga tacatttatg gttatgcccg
     7201 agtgagtaca cgttcacaag aactaaatcg tcaattagac ttactcaatg aacaaaattg
     7261 taatgaaatc ctaactgaga aaatgacggg aaccaaagcg gttcgtcccc aactaaatcg
     7321 tttaaaagat aaaattcgtc cgggagatac ggtaattgta gaaagttttt ctcgattggg
     7381 tcgtagtaca aaggatttga ttgaccttgt tacctatttt gaagaacacc atgttaagct
     7441 ggtgagcttg aaagaaaact tcgataccag cacgcctcac ggacgcttaa tgatgactgt
     7501 cttccaagca tttagtcagt tcgaacgtga tttaattgtt gaacgtacca aagaaggttt
     7561 gaaaagtgca cgtgcgagag gtcgtaaagg tggccgtcca cgagtgaacc agaaagatgt
     7621 tgatcgagca gtaaaattgt ataaaagtca ggtttatagc gtaaaagaaa ttacggaaat
     7681 gactggtatt agcaaggcaa cactctaccg ctacttaaaa gacaataggg aataatctat
     7741 tttaatcttt aggaatctat aatatttatg ggagaattag agtgattttt ttattattac
     7801 taaaaaacaa tataaaataa cgcattttaa gccctctttg tgcataagga tgaagctttt
     7861 aatttgaaag aggcttaaaa tgcgttttaa ttttttctat ttttcataaa tatataggtt
     7921 agtggtttaa aattataggg tattacaact ctcagctgtt aaaaattatc ttttaaagtg
     7981 tcgtctatcc tctcaagttg aaacattggg ttcttaccta atctttttta attgttcata
     8041 aaaattttca tgcgttccac atagtaagat aggtatcact gcattatctt taatttcata
     8101 tgcaacgcga tatgtcgtac ctgcatactt gaatccaata gcccaaattc ctgataaatc
     8161 acccgttttt tgttctcccg aatgaggacg aactgctatc tcatcataaa ttatcgttaa
     8221 aaattgttct tttaatgttt tctcttttaa tttttttaga tactttatta cgtgtttaga
     8281 aggtagaatc tcataagcca atttctgctg ccaactcctc ttttgtcatc ggtttagaat
     8341 ttgcaacagt atcttcagca atagatttaa atgcagaagt taacgcattt tttctatttc
     8401 taaattcttt taagatctct tgtttgctat aaccttctga aagaatatca gccaaaatat
     8461 cttcactaaa atcaaaaaat tcttttttag gctctacaaa ctcataaagg ataccattct
     8521 ctacaagctt agcttctact tcaacaccat tgggcacatc gaattcttta gggaccgtta
     8581 acataataga atttccttgt tttcttgttt ttgtaatcat attatttcct ccattatttt
     8641 acatttcgta attacttagt aattacattg tacgctattt ttagtagaaa tacaaaataa
     8701 taaaactcct ttagaacaat tttcaaagga gttttatttg attaggaaat tatgtagaat
     8761 aataatatgt ttttaacttg gaatcaaaaa cgcacttgca agtaagttta cttcaataca
     8821 tgaaattgct tataatagtg tattggaatt aaggtagatt gacaaaagag tgatcaaata
     8881 ggtatacttc attcaaataa tcgtttgaat gtgaggtgag atgaatgaaa aaagtttgcc
     8941 aagtgactgt cgtacataaa gaaaaagtaa atggaataaa aaataaattg gatcaaaaag
     9001 atttttcaaa cttacttgtg ttagggaaat gtttcagcga tttttctaga attaaaattt
     9061 tctatgcttt agaaacctat aaggaaatgt gcgtctgtga tttagcagaa atacttactg
     9121 ctagtgtcgc tacaacgtca caccatttac gttttttaaa aaaacatgga atggcaaaat
     9181 cacgtcaaga tggaaaagta gtttattact ctttagcgaa tgaagacatc ctttccgcta
     9241 ttcgtgtttt ttaaagttat cagaaaatct atctatgaaa tcttagtttg ggggtaccgt
     9301 caggaaaatg cggatttaca acgttaagga aatttattaa agagaccaat tcctcagcac
     9361 taaaattgat aattgggctt tctttgttac tatagtgtta ggtaagcttt ttactttatc
     9421 accggttttc ccctcacttc acacctacgg gcgagtttcc ccacatcccg ttttccatct
     9481 aatccaattc attcaatatc tttatgtgta tgaaagatca gattgcttga tttagacatc
     9541 ttacacgtaa tttattctta aatgggtacg gtaaacgagc aataatacga gcaaaagtat
     9601 acctacaaaa ataataactc tgtctaattt tttttgattc atatgcttat tgcttaaacc
     9661 atataatttc agtccaagaa agcctccaac agtatttgta attacatctg ttatgtctgt
     9721 cgctccaata gcgaagataa attgaattat ttcaaaagta agacttaaaa ccagtacaaa
     9781 agcaaactta ggtaaaaatc cgatttcttt aaaattgaca ttcaaaagca agccaaatgg
     9841 aataaagatt ataacattat ctatcatctc tctgaaattc ccagtagcag taaatggagt
     9901 caagttaaga cttctttgat gataattaaa tactgataaa atattgtatt gtaatttgaa
     9961 taacactaac cagattagtg tcactaaata taaagctagc aatcctctag ataatatttt
    10021 tcccaatata atttcctcta attcttttgt ttaagttgta tcattagtat agcaatgtcc
    10081 tagttaacgt gggttttaaa ttaaaaagct aacgttaccc caaaagtgct agtacaataa
    10141 tgctgttcat taatttcgtt ggtttcattc cgccatcctt acctccttga attagtatgt
    10201 gttgatccgg gaaacactgt cacaattaca ccgtctatat tgtttcctga tatctcataa
    10261 cgaagattag tcggcacatg aatggtggta tttttagtaa caggataata aaagatctca
    10321 tatttttccc catttacact aacagaaatg gttttttctt cttttaggta atccatatat
    10381 tcctcgagaa cgaaattctt ttctttcata atcgcactat gtggtaaacc aacatagcga
    10441 atatgccatg gttcatattg aattcctgtt aactctgttt tgtcctctgg ataacgtaaa
    10501 atgaacccgt atttccaagc attttcttct atccactttc cttcaggggc tcgttccatt
    10561 ttcgtcaagc ttgatcctac atctagtgat aaacctgaat tatgctcact ataacctgct
    10621 ggtaaggcat actcagcccc catttcttgg taaagcacac tttgctcatc aaagtctcga
    10681 tagccactat taataataaa atgactaacg ccacccttta cagcatcatt gaccatctct
    10741 gaaaattttt gtgctatttc ttttgacata taaatattac tatcaagcaa cccgtatcca
    10801 tttattaatt cgtcatgttt agataaattc acgatatctg acttcacact ttcttggcga
    10861 acaggatatt tactattgat taatagcaga tttccttgat aaacctgttc ttctgtaatt
    10921 gttttctctt gggtattttc agaagtccca ctattttcta aatgttcttt gggattttga
    10981 tcataatttt gaaattcgac tttttcctga gaaaacagtg cttcattaac gtagtcataa
    11041 cctaagtata ttaagaataa caataacaat aaaaaaaaca acttcttcat tttcagtctc
    11101 ctccttaact
//
LOCUS       DACGLW010000092        11047 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.097, whole genome shotgun sequence.
ACCESSION   DACGLW010000092 DACGLW010000000
VERSION     DACGLW010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11047
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.097"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            complement(63..1268)
                     /locus_tag="IXT53_002450"
     CDS             complement(63..1268)
                     /locus_tag="IXT53_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HAQ4906233.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
     gene            complement(1291..1444)
                     /locus_tag="IXT53_002451"
                     /pseudo
     CDS             complement(1291..1444)
                     /locus_tag="IXT53_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            complement(1447..2832)
                     /locus_tag="IXT53_002452"
     CDS             complement(1447..2832)
                     /locus_tag="IXT53_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAQ4906234.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            complement(2861..3247)
                     /locus_tag="IXT53_002453"
     CDS             complement(2861..3247)
                     /locus_tag="IXT53_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ4906235.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            complement(3263..3577)
                     /locus_tag="IXT53_002454"
     CDS             complement(3263..3577)
                     /locus_tag="IXT53_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HAQ4906236.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            complement(3930..4409)
                     /gene="rlmH"
                     /locus_tag="IXT53_002455"
     CDS             complement(3930..4409)
                     /gene="rlmH"
                     /locus_tag="IXT53_002455"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HAQ4906237.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYGLAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            4837..6126
                     /locus_tag="IXT53_002456"
     CDS             4837..6126
                     /locus_tag="IXT53_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HAQ4906238.1"
                     /translation="MDRKNVTPKMKKNKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVMMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(6165..6770)
                     /locus_tag="IXT53_002457"
     CDS             complement(6165..6770)
                     /locus_tag="IXT53_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HAQ4906239.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVASYGMICSARELHLPNAPEKKGILVLPEDAVVGEVFPK"
     gene            complement(6966..7430)
                     /locus_tag="IXT53_002458"
     CDS             complement(6966..7430)
                     /locus_tag="IXT53_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ4906240.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKALEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKQMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYAIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(7452..7772)
                     /locus_tag="IXT53_002459"
     CDS             complement(7452..7772)
                     /locus_tag="IXT53_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HAQ4906241.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VIKG"
     gene            complement(7915..8994)
                     /gene="pepA"
                     /locus_tag="IXT53_002460"
     CDS             complement(7915..8994)
                     /gene="pepA"
                     /locus_tag="IXT53_002460"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HAQ4906242.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            9194..9544
                     /locus_tag="IXT53_002461"
     CDS             9194..9544
                     /locus_tag="IXT53_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HAQ4906243.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKTSFLKEGAIEGAWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLDISRKKGAID"
     gene            9729..10682
                     /locus_tag="IXT53_002462"
     CDS             9729..10682
                     /locus_tag="IXT53_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HAQ4906244.1"
                     /translation="MESREKIAVLIPCYNEEATISTVIEDFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAAHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVKNTITYLYKTKIRDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMCKDYKPLLFFGIWTIFFFLFGLVMGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYQNKEMK"
     gene            10695..>11047
                     /locus_tag="IXT53_002463"
     CDS             10695..>11047
                     /locus_tag="IXT53_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906245.1"
                     /translation="MHKKKENLFPTFFATLFASGFAFFTSYGLGTLNPTVIIGLAMVL
                     LLAFKQYGIELFSIKNKVAAIARLFFSAAFALTLSIGRQIQFQYVSANYTKTTIADGS
                     IFYFLVYFSIFVYSF"
BASE COUNT         3167 a         2209 c         2054 g         3617 t
ORIGIN      
        1 aggttttgtc caaaattcat aaaaatgttc tcctctctat attcaatgaa tttgtatttg
       61 agttattttt ttgttgttat cacgtcctgt tcttttactg actgttgctt caaaatctgc
      121 ttgtgtcggt ctgtcagttt cgcatggtcg agaatgtctt ttacaacctg cgtctggttg
      181 atttcatcaa gtttaatcgc aacctttaag gtcggggcaa cttgatgaga tagccagttc
      241 agcgtccttt ggaaggagta aggctctggt tttgtggtta gttttaatcg ttcacgattg
      301 ttcccaataa accaagccca ttcttcattc agtttccaat cagaacgagg tttggaatcg
      361 tctttatcta caaaacggat ataccgattg ataattttaa aggcggtatg ctctggattg
      421 tcatagacga gtaaatcacg gactgcataa taggcacgct catttttcaa tcgaatctca
      481 aaacggtttt ttacttctgc gtcttcaatg ggaatatcat ttttcttgta ctgctcgtag
      541 tccttttcat agatacagaa ataaacttca ctttgtaatg aaccgatata gagggtgttt
      601 cccatacatt ccttttcctc tttgcgtacc agttcgccac tgcgatagct tttaaaactg
      661 cggaagacgg agatacattc ttcctgttgg cacttttcag tgagtacagg gatatttaaa
      721 atccctgtct tatcgttaat ggcaaggtca aggcgtttca tcacaccgcc agccaccaaa
      781 acgtccataa agaactcata ccagcttctt tgttgtgcca gaagatagct ttcaaattgt
      841 ctgcacccac gacctttcaa ttccaccaga actcctttgt ccagttcatg ggagcaaagg
      901 acgaatatgt cgcctaaagc ataatgctct gaataagaat agaaaccata gtcctcatga
      961 agaaaatagg acagtttcag ttgtaagatg ttttcgacca cctgctgtac gtctgttgtc
     1021 ggaaagcgaa ttcttacata atcaaacagc atttcaaggg gagcgtcggg attgaagcgt
     1081 tccagagctt cccaaaggga ctgctgtaaa tcctctgatg gcttgacttt tcctgtttca
     1141 atatcgctta gatactgcct tgtaatacca gtcgcaacag ctaaacggtt ttgagatagt
     1201 ccataagcca agcgtttttc ttttaaatgc tgtaaccaag tttgttcatt cagtaaaaat
     1261 ccctccaatc aaaaaggcgt atgtcaactt ttaaagccca tttgacatac gctgaaattt
     1321 tgtaaatccc ttgtaaccaa aggattttct aatgtttttt tgactgtttc ctgtcgattt
     1381 gtacccccct gttagatacc gaggggttaa gtgctggcgt ggctattgcc acaccagcca
     1441 gcaagatcag tccacacctg cgacttccgc ttcgcacgtc gcctgcgtgg actgtctgct
     1501 gttggataac tttttaattt cctccaagaa atcatatcct tttggtacaa ggggagtata
     1561 aaactctgat atgacacttg ttcctacatc aacatagcca cgacctttga ttcgctttaa
     1621 gaagaaatcc ttttgtacgt cactgccaaa catcatgcca tagcccattt cagacatacg
     1681 acctaaagcc actctgaaat taaactgatc acggattccg tcgcctaaat attttgcgtc
     1741 tggacgttga caagccagta ttagaaagaa gccagcttga cgacctaaca tgacaatctg
     1801 tttcagctta ttcataactg cggtgttttc ttttgttccc agcatttcca tgaaagcgac
     1861 gtattcatca aagattaaga agtgtgccgg gagacctaag taagcataat ttttgccagt
     1921 cttatagttc ttcatctgct tcatttcctc actacgtttc atcatttctt catagaatgt
     1981 ttcaatgcaa gaaagcaagt cttcttttct atagtagaca tttgccatca cagaacctaa
     2041 gtccgcaagg tcagcatttt tcgggtcaag aatatacagt tttgaatctg tatgaagcaa
     2101 ggcttcaatc agtgtcagta taaagtaagt tttaccgcca cctgtaccac cagcaatcaa
     2161 catatgaggg agcttatcat attcccacca tacgtttttc attaagcgaa gtttaccatc
     2221 tttagcttct acttcatcaa tagaaatacg actggctatg gtgtcataga gcaaagtata
     2281 ttccacatag gaatccttta actctttatc cgtcagctca cagtacaagc cactctctaa
     2341 tttcttttcc aagtgtaaga gttggtcttg atattttccc agcgtgattt ccacccgtat
     2401 ctgtatcaag ccatttttaa gtcgataata cattttaggg aagtaggtta tcttttcctt
     2461 tgtacgacca gcactatctt taaagaaacc ctctgttttg acctgttcag attcatacca
     2521 cttgttttca agtatcatct ttgccagttt ttgacggtgg taaagttgtt taaccgtatc
     2581 atagcgaacc cgtttgaata caaacgctac cagcaagcag ataagaattg cgacactgaa
     2641 actgataatt aaatagggaa tgtcaatctt atctgcttgt gataggttaa aatcctgcca
     2701 gttgatctgc tggattgtct tcacatgaaa cagtccgaca accagcagga aaacaggcag
     2761 gagtgacgct atcgtaaaat gaaagactaa atctttacca gatgggcgaa tccttttacc
     2821 acgctgtttc atgcgaaaaa gtctcctttc tacctagcga ctatttgtct tgtgtcggtt
     2881 ctttctttgc ttgtggttga gctttgaatg aactagaatc ctttgtcagc acaatatcgt
     2941 ctgccttgat ataccagtca acatctgctc cttgataggt ggcagtagca acggtgtccg
     3001 caatgggatt gataagttcc acccgtgcgt tataatcaaa ctctttcaaa ggcacgctgg
     3061 caggaatact tacttgaatc atgcgtcctt gtcctttgga ttttaagtca taggtacgtt
     3121 ccttgatttc atctgaaacc gacccgtctt cattttggat tctcacttca cgacgtagag
     3181 cagagaattt caattctcca aaagtcgtgt ctttatctaa tacaatgcca tttgctaatc
     3241 tcatcatttt tcctctcttt ctttattctt ttatcatgtc gtcagcatgt aaaaggtaat
     3301 ttgtaaaacc acgagtgccg attttgtagc cctctgcggt aatacgtgga ttgactaact
     3361 tcacacgttc ctcaaagccg aaatgttttt cgccagcttc agcaggaagc accaccacaa
     3421 tatcatctgc tctttgaaca tcagaataga gattatagct tcttgataag acagttagcc
     3481 gtccgttgat tcttcgctga acgactttat cctcgccagc aaattctaaa ttgccgaatg
     3541 ttttttccat gttgggaatc acaaatttaa gttccatatt tttacctatc ctttcttttt
     3601 tattggctga atgaatgttt gatggtctta aagagtgggg aacgaccttt tgattcttga
     3661 ttttttgttt tcataagttc acttcctttc aaaatcgggt aaaaaaatag acacctcatt
     3721 ttttgaagtg tctacctatt aaatattcaa attttattgg aagtatcttt atatcttcac
     3781 ttttcaagga taaatcgtcg tatcaaagct cattcataag tagtaaatta gtagtaaatt
     3841 gagtggtttt gaccttgata aagtgtgata agtccagttt ttatgcggat aactagattt
     3901 ttatgctatt tttctcttaa aaaatatcat tatttgtggt atggagcccc cgcgttgatc
     3961 cggaaggcac ggtagatttg ctcgacgagg atcaggcgca tcaattgatg tggatacgtc
     4021 attttgccaa aagagatctg agcattgctt cttttcatga cggcttcgct gagtcctaaa
     4081 gaaccgccga taacaaaaac aaggtggctt tttccttgga tacctaattg gtcgatttgt
     4141 ttagcaaaag attcgcttgt gggatttttc ccttcaattg ctaagccata gacgtattct
     4201 tgttctttga ttttcgctaa gatccgttct ccttcttttt cttttacttg tctcatttgc
     4261 gcttcgctta aattctccgg ggctttttcg tcaggaactt cgatcaattc gattttcgca
     4321 taggcattca gccgtttgac atactcattg ataccttgga tcaagtattt ttcttttaat
     4381 tttcctactg aaataatttt gatattcatg ttttctctcc tttgatcttc acgctcactt
     4441 tgtttattat aatcaatttt ttcgtctata tgtttatttt gactattttt gttaaaaaga
     4501 tttccccaac agttatgcac aacatccaca ggtttatcca cagaaaacag cgttgtttta
     4561 ccttatttca tcacattcat taggcaaaca tacgttttat aagaatttat ccactttata
     4621 cacaggctgt ggatatgttt tatcccttat ttttccaatc ctatccgcgt agttttacac
     4681 aatatctgtg gaaaaagaaa atcaaatatt ttcatctgaa aacaatgatt tttcctaaag
     4741 tttttcataa aaagttcaaa gtctcaaagt agactaaagt caacaaataa gactatacta
     4801 acagtagata aatgaatgta attggaggag gaacacatgg atagaaaaaa tgtgacacca
     4861 aagatgaaaa aaaataaaaa taatagcctc tggcgtaagt tgggtctggg gcttgttgga
     4921 gggatcgttg gaggactact gacagctggc attttttatg cagcgatggg cactggcaac
     4981 aatagttcta ctgctacaag cggcaatcaa aattctgccg gtgaaacagt tgtggaaaat
     5041 gtcaaagtca atgtagattc tgatatcacc aaagcggtag ataaagttca agacgctgtc
     5101 gtttctgtta tcaacttaca aagtcaaaac caatcaagcg gttttggcgg attattcggg
     5161 cagcaagaag aaagtagcag ctcagatgat agtaatttgg aagcttatag tgaaggaagc
     5221 ggcgtaatct acaaaaaaga cgggaatact gcttatgtag tcacaaacaa tcacgtagta
     5281 gatggacagc aaggtttgga agtcatgatg aaagacggta cgaaggtaaa agctgaatta
     5341 gttggaacag atgcctattc agatttagcg gtgttgaaga tcaattcaga caaagtcgaa
     5401 acggttgcct cttttgggga ctcaagtgct ttgaaggttg gtgaacctgc gattgcgatt
     5461 ggttccccat taggatctga atatgcgaat tctgtcactt caggaatcat ttcttccttg
     5521 aaccgacaag taacaagtac gaatgaatcg aacgaaacag tcaatatcaa tgcgatccaa
     5581 acagatgcag cgatcaaccc gggaaattct ggtggtcctt tagtgaatat cgaaggacaa
     5641 gttatcggta tcaactcaag taagatcgca agtacttctg aatcgacttc taatgtaagc
     5701 gtagaaggaa tgggatttgc catcccaagt aacgatgtag tgaatatcat caaccaactg
     5761 gaaaaagacg gaaaagtgac acgtccagct ctaggtatca caatggtcga cttatctgcc
     5821 gtttctactc agcaacaaga acaaatcttg aagattcctt catctgtaac taacggtgtc
     5881 atcgtgacaa atgtccaaac agctacaccg gcagaaaaag ctgggttgaa acaatacgac
     5941 gtcattacta agatcgatga caaagaagtt tcttctggtg tagaacttca atctgctttg
     6001 tataagaaaa aagtgggaga ttctgtcaaa atcacttact atgaaggctc gaaagaaaaa
     6061 acaactactg ttgaattgac gatcgatcaa tcggcattaa aacaaaatca aaatagtgaa
     6121 aattaataaa aaagaggttg tgacaaagac ttgtcacaac ctctttattt tggaaatact
     6181 tctccgacta cggcatcctc aggcaatact aagattcctt ttttttctgg agcatttggc
     6241 aaatgcaatt cacgagcaga acaaatcatt ccatagctgg ctacccctcg caaagttcct
     6301 ggccagatca tcattccatc tggcatcatg gctcctggtt ttgcaacgac aactttttgt
     6361 ccagcttcga tatttggtgc accgcaaaca atttgcaata ctgtcccatt gtccacttct
     6421 gtctcagtaa tcgataaatg gtcagagtca ggatgcggga cgcatgtttt tacatacccg
     6481 acaacgatct tcggttcatg atcagcttca agctgttctt cgaatcctgc ttctttaatc
     6541 aatcgattca atttcgctaa ttcttcatct gttaagctta cttggccgtt cccttcaatt
     6601 gttagatgat cggagatatg gaaaaagttc caagcaacaa cttgaccttg ttcatctttg
     6661 atccgagcaa cattccattt acgttcgaca gtgttttctg ttcctttatc gtcggctatg
     6721 ataaccatca aagtgtctcc gacgtgtgct ttgttataag caaaaatcat gttctttcct
     6781 ccattatcct ttgctgagat ttatcttaac aaaaaattgc attcactgca tgagattttt
     6841 ttcatgcttt atttccgaag aagcttcttc tggaacacca tttattcgta acaagggact
     6901 gtgacaagac ttttgtcaca gtccctttac tgtttgatta aaaaggttat acaagttcca
     6961 cttgcttaat tactcggctg ttcttcttcg tcagaaacga tcaagacatc acaaggtgaa
     7021 tgacgtatga cgtagctggc tacacttcct gtcataaagc gttcgactgc atttagtcca
     7081 gactgcccaa ccatgattag atcgattgca tatttttttg gcaatttgac tgccatggat
     7141 tcacgaacag agccgtatgc aataattcct tctatatttg tgtaatcaac agattttcca
     7201 tattctttac attcttcgat catttgcttg gctgtttcag tttgctgatc gactagattt
     7261 tgatcaatag gagcatagcc cataaaatta taaaattgct gatcgattac gtttgctaca
     7321 tatactttcc cattattacg gcgagccact tctaacgctt ttttgaaagc agcaagtgct
     7381 tgttcactac cgtcaatacc tactaaaatg ttcttatatt cttgattcat cttgacccct
     7441 cctaacagat gtcagccttt tattacactt tctaggaacg attcgatttc ctgttttgtt
     7501 ttacgatctt tattcactag acgacctaat tcttttccgt cttggatcac gacaaaactt
     7561 gggataccaa aaatgttcca ttcagcggct acatctatgt acttgtcacg gtctacttca
     7621 ataaattgcc aatcagaaaa gtcattttcg atttcaggca tttgcggttt gataaaacgg
     7681 cagtctccgc accagccggc agtaaagaaa aatacacttt ttccagtttc tacgtagcct
     7741 gctaattctt ctaaactatt tgggataatc attctttttc ctctactttc tgtttatgta
     7801 tttttcaatt aaattataca cctttctttc gaactaacaa acattgatcc tctttttata
     7861 aaaaaaatct gacaaaaggt tgcttttgcc agattttata gtttcactat cgcgttattt
     7921 cccgtatacg atcgtgttca ctgttgattt atctaaacct ttcaatgttt gaatcaacat
     7981 ttctcgtgca gcttcaaaat cgcgaatact gaacatcgtt tgatgcgtat ggatataacg
     8041 tcccactacg ccaatcactg tacttgggat tccttcattt tgtgtatgag cagcacctgc
     8101 atctgtaccg ccttttgaaa caaagtattg ataagggatg ttgtttgttt cagcgatatc
     8161 tagcaagtac tcacgcaaac gaggaagcat gatcaaacct ggatcttgga tacgcatcaa
     8221 tgtaccttct cctaaatgtc cgaatgtgcc atttttcgtc actgtgtcat ctgctgcaga
     8281 gcagtctact gcaaagaaca gatctggctt gaatttattg acagaagctt tagaaccacg
     8341 caagcctact tcttcttgga cattcgcgcc tgcaatcaat gtatgaccta atgtttcatt
     8401 ttgtaacgct tctaaagctt ctaataccat tgtacagcca tagcggttgt cccacgattt
     8461 gctgatgata ttcttcccgt tggctgtttt gatcgtttca gcgtaaggaa cgattgtgtc
     8521 tcctggaaga acaccgtaac tcattgcctc ttctcttgat tcaaacccag catcgaacaa
     8581 aatatctgtt acttctactg atttttgtcc gcttgttcct cgtaataaat gtgggggaac
     8641 ggaagaagaa atacatggat aattcttacc agtagaggtt ttaagtgtaa aacgttgagc
     8701 agaaacaacg taagggttcc atccgcctag aggtacgact ttaaaaagcc cgttttcttg
     8761 gatttgtgtc agcatgaatc cgacttcatc catatgtgct gcgaccatta cacgaggtgc
     8821 atccgcttct tcatggtggc gcagcccaaa aatgccgccc aaaccatcta actgtaactc
     8881 atctaccagc ggttccatat gactttccat ataggcgcga acgtcttgtt caaaaccgct
     8941 tgttccttgt aattctgtta actctttgat tcgttgaaat gttttttctt ccatatgcta
     9001 ttcgtctaaa tttcagacta aactcctctc agacttatat tgatctgcgg ataaaatatg
     9061 attttttctg cagaccgatc gtatttatta tagcttattt tttctggtag cggttattta
     9121 tttcaaagaa aatatgagag tgtggtaaaa taaacacaaa gagtagcaag tgagaagaag
     9181 gaggtatatc cacatgtatg aagaaagcga attaagctat tttaaaggcg ggttagccgt
     9241 tgggatcggt ctaggattat tgagtggggt agcttcaacg atttggtatc atcgcaaaaa
     9301 agtgttatca gcagaccaag tgcttgaaaa tatcaaaaca tcttttttaa aagaaggcgc
     9361 aatcgaaggt gcttggatct cttttgaaaa agaacctatt cggaaattcg ccgttcggtc
     9421 gagtgcttat cggggtgggc tttcccggat agaagatggc gaaactgttt attatgaatt
     9481 tttagcagat gctcatacag gaaccgtatt agacatttca agaaaaaagg gcgcaataga
     9541 ttaatacgtc aaaaaaatga aataacgaat acgcacccac tatctaaaag agaggtatcc
     9601 tatccttctt ttatagtggg tgatttttat aaaaataggg ctatttcctc ttggaatagc
     9661 tagtatttgt agttattata cggtattctt gaaataagat tagttttatt atataaggag
     9721 agtgacaaat ggaatcacgt gaaaaaattg ccgtgctcat tccttgttat aacgaagagg
     9781 cgacaatttc aactgttatt gaagatttta aaagggaatt accagaagca gatatttatg
     9841 tctatgataa taattcgacc gatcgaacat acgaattggc cgttcaaggc ggagcaatcg
     9901 taaagaaaga accacgtcaa ggaaaaggga atgtaatccg tcaaatgttt tttgatattg
     9961 atgccgatta ttatttgatg gtagatggtg atgatactta tcctgcagaa gctgcacatg
    10021 gtttgcttga gaaattacgt tctggcgaag cagatatggt cattggcgat cgtctatcaa
    10081 atggaaccta ttttgatgaa aataagcgtc ctttccatga ttttgggaat aatctggtta
    10141 aaaatacaat tacttactta tataaaacaa aaattcgcga tgtcatgacc ggctatcgag
    10201 gattcaatcg aatttttgtc aaaagcttcc ctattatgag ttctggtttt caaatcgaaa
    10261 cagaattgac gattcatgct ttagataaaa aattcaagtt cgttgagatt ccaatcgatt
    10321 atcgcgatcg tcctgaaggt agcgaatcaa aattgaatac attctctgac ggattcaaag
    10381 tgatcatgat gatcatcaaa atgtgcaaag attataaacc tttgcttttc tttgggatct
    10441 ggaccatctt cttcttttta tttggattgg tcatggggat tcctgtgatt cgtgaatata
    10501 tgctgacaag ctttattaca cgcatccctt ctgcgatttt aagtactgga ttgatgactt
    10561 tagcccttct ttcattagta acagggttga ttttggatac ggtcgtcaca aatgccaaga
    10621 aagaatatga attgaattta taccatgtct accacgaata tcaaaataaa gaaatgaagt
    10681 aggaggcctt ttttatgcat aagaaaaagg agaatctatt tcctactttt tttgcaactt
    10741 tatttgcgtc aggttttgct ttttttactt cttatggtct aggaactttg aatccaacag
    10801 taatcattgg tttagccatg gttttgctac ttgcttttaa gcaatatgga atagagttgt
    10861 tttcaataaa aaacaaagtt gcagctattg cccgattgtt tttttctgca gcttttgctc
    10921 ttacactttc tattggcaga cagatacaat ttcagtatgt ctcagcaaac tatacaaaga
    10981 caacgattgc tgatggaagt attttttact ttcttgttta tttctccatt tttgtgtatt
    11041 ctttttt
//
LOCUS       DACGLW010000093        10760 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.098, whole genome shotgun sequence.
ACCESSION   DACGLW010000093 DACGLW010000000
VERSION     DACGLW010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10760
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.098"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            complement(59..1042)
                     /locus_tag="IXT53_002464"
                     /pseudo
     CDS             complement(59..1042)
                     /locus_tag="IXT53_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311512.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            complement(1437..2279)
                     /locus_tag="IXT53_002465"
     CDS             complement(1437..2279)
                     /locus_tag="IXT53_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HAQ4906246.1"
                     /translation="MKGDVIVEIKVLFENSRINLNFKSGHGLSLLINYSGKKYLFDAG
                     ANKKLVYNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLK
                     ILTTFLYVGIPKNEILKMHENLHTIKTKTELAPSFHIIPLGKANSTTKNLYKKMDDKF
                     VYDDFSDELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMIGI
                     PYINNLGLPEQEVMKIGNKLNDSRVEKILTCHCTGSKAFNILKTQLGNKLEAIKTGQH
                     LEIS"
     gene            2604..3020
                     /locus_tag="IXT53_002466"
     CDS             2604..3020
                     /locus_tag="IXT53_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAQ4906247.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIKESQQSVIDCRKLLVEQLTGGDDL"
     gene            3017..3487
                     /locus_tag="IXT53_002467"
     CDS             3017..3487
                     /locus_tag="IXT53_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016622974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ4906248.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            3501..4274
                     /locus_tag="IXT53_002468"
     CDS             3501..4274
                     /locus_tag="IXT53_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ4906249.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAASPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            4288..5100
                     /locus_tag="IXT53_002469"
     CDS             4288..5100
                     /locus_tag="IXT53_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ4906250.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            5154..6119
                     /locus_tag="IXT53_002470"
     CDS             5154..6119
                     /locus_tag="IXT53_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ4906251.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            6256..8979
                     /locus_tag="IXT53_002471"
     CDS             6256..8979
                     /locus_tag="IXT53_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ4906252.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESQILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGGSIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVGNLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            8992..9624
                     /locus_tag="IXT53_002472"
     CDS             8992..9624
                     /locus_tag="IXT53_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ4906253.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            9829..10056
                     /locus_tag="IXT53_002473"
     CDS             9829..10056
                     /locus_tag="IXT53_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009320234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HAQ4906254.1"
                     /translation="MLQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEV
                     IPENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            10125..10391
                     /locus_tag="IXT53_002474"
     CDS             10125..10391
                     /locus_tag="IXT53_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HAQ4906255.1"
                     /translation="MLVQMMNGGRIMSQSTITFRIPDDEKELVLEYAKAHNSSLTELY
                     RNAVLDKIEDEIDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            10381..10650
                     /locus_tag="IXT53_002475"
     CDS             10381..10650
                     /locus_tag="IXT53_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ4906256.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
BASE COUNT         3565 a         1716 c         2103 g         3376 t
ORIGIN      
        1 aataaatagt ttttctatca agtatattga actacgataa atttgttatt ttgttttgtt
       61 taatttcgtt gtacttattg gctttgactt cgatttcttt aaatactggg taatcggtgt
      121 ccggcaaagc tagcaaatct gttttttctg tgttccatag atcattgata atgacatgtt
      181 tttcgtaatg gattcgctct cgatcgtgtg acagctccac cgctaatgcc tcgttgaaac
      241 tggcgaaatc cttcataatc ggtgtagatg aaaagaagtt gtaccgtaca tagccgactt
      301 tgttttccat actacctttc tcgtttccgc tacgtggatt gcacacctgt gtctcaaaac
      361 catagtgcat agaaaatcgg aggaaaccgt cagtcagcac cgcttgttca gtctttgttt
      421 ttgtctaagt aacggccgtt gacatattat caatccgtat tttccgagga acgccacctg
      481 attggctgaa gagttctttc atgccttcca acaaacattc ttgattctcg gcggggagta
      541 caacggcgaa tcctgcatta ctaaaaggga aagtcatcac taataccttc acatctttaa
      601 acgctccatc gtgacaaact tccatcgtac caaaatcaag ttgcgcttct gctggcgggt
      661 gttcaagacg ctcaaagcct tggtcttcca gtgtttccaa gtagtgtgtc gctttccaat
      721 cttgaatgaa tacacagact gttcggtagg atcctggaaa gccatcggct tttaaagcag
      781 tagtcattgc ttcagcagtc ctacgctttt tatttggcag cttagcatct tctgaaagcc
      841 atagggaaac gatgcctccc cacttttctg tatacatcat accagtcttt ttgcgtgctt
      901 taggttgcgg cagcaaatca tcatctgcat attttttagc cgtgcgccaa tttacatcta
      961 gctcctttgc aatatgatta atggatttat catgattatt tcgtaaattt ctgatagtat
     1021 taatatcggt cattgtgagc atacctttct tacctccagt tacagtgtcg agactataac
     1081 tttagaggag tttcaggttg cccgcaatgg ctttttatct taacttaatc gatttgcgga
     1141 gttgtgtagt ttttcgttgc acttgtatgt acttttattc tgccataaac agcttaatga
     1201 atgtccactt gaaaaagtga ataaacgaaa tagatgactc gatttaataa ggaagtaaac
     1261 ccacacagaa atataggtga ttttccttga atgaccaaga tattgaaagt tagtgaaaaa
     1321 acacaaaaag gtagccctat tcacagaagg tctgcctttt gtgttatcgc cagcaaacat
     1381 tagagggggt atttcatata gcttttgtcc tctcgacagt cctatcagtt tctcttttaa
     1441 cttatctcta agtgctgtcc agttttaata gcttctagct tgtttcctaa ctgtgttttt
     1501 aatatgttga aggcttttga acctgtgcag tggcaagtaa ggattttttc tactcgtgag
     1561 tcattcaatt tatttccaat tttcattacc tcttgctcag gtagacctaa attatttata
     1621 taaggtatgc caatcatatg gaagccacca attaggtatt ttatgggttt agtcggaaat
     1681 gtttcaagag caatttccat catatcgata atgccatgat gactacagcc agtaaagaca
     1741 atcagaccgt cttcttgctc aattacgacc aataattcat ctgaaaaatc atcataaaca
     1801 aatttatcat ccatcttttt gtataaattc ttggtggtcg agtttgcttt gcctaggggg
     1861 atgatatgga agctcggagc aagttctgtt ttcgttttta ttgtatgtaa attctcatgc
     1921 attttaagga tttcattttt aggaatacca acatacagaa acgtggttaa aatctttaaa
     1981 taatggggta aagttacacc ttcagctgta taaatagggg cgtttcctga ttttaaatat
     2041 cttaacccgc cggtgtggtc agaatgaccg tgtgaaataa tcactgcctc cagatcattc
     2101 aaagtttccc ccattttagt cgcattatag actaattttt tattagcgcc agcatcgaag
     2161 agatatttct tccctgagta gttaattaag agagataacc catgtccact tttaaagttc
     2221 aaatttattc ttgagttttc aaataaaacc ttgatttcca ctataacatc tcctttcaca
     2281 tgaacgtgta ttcatatatt gtagcacatt aagttctatt ttttgttgta attaccgaaa
     2341 aaaatattaa tggacgcttt ttacgcttta tttcgtatgt ggaagaaaat ttcttaggat
     2401 gtggcggggg attggggcaa agctaaattc caattacagt gatcatttaa ttagacacct
     2461 ttaaataccc acactttcat caacactata atttagggaa agaaaagtgt tggcatgata
     2521 tagaaaaacc tttaattaac agcgtttcct aaaattggca cgcatcttgc ttatagaaaa
     2581 gcgtaaggat aggaggaatg aaaatggcaa ctataattct tgccagccat ggggaatttg
     2641 ctcaaggatt aaagcaaaca gcgacaatga ttattggaga tagtgtacca attcatgcct
     2701 tgtcagcatt tagagatgaa gatgaatcta ttttggtaca aataaaaaaa cttttagcca
     2761 caatagatat agaagaaacc tacattttaa ctgatattct aggtggtagt gtgaataatg
     2821 atatgctcac tattattaaa gagtatgagg gattgcaatt aattactgga atgaacttac
     2881 ctttagttat ttctattgca acctataatg gaaaaattgg ggaaagtgag ctagaaaagc
     2941 ttattaaaga gagtcaacaa tcagtgattg attgtagaaa attattagta gaacaactta
     3001 ctggaggaga tgacctatga ttaagttagt acgtattgac catcgtttgt tgcacggaca
     3061 ggtagtattt tcttggagta aatcattaaa tatcaatcgt attattattg ccaataaaga
     3121 ggcagcgaat gatgaattca aaaaaatgtc tttgaattta tctaaaccga ctggcattaa
     3181 attaacaatt ttttcagttg aacagttact agaaaaaatg cctaaaatcg aaacacttaa
     3241 cgaaaatatc atgcttattt ttggtaatac aaaagaaatt cgtcaatttt gtgaagccta
     3301 tcctaaagta caagaaatca actatggagg gataatcaaa aaagaaggtt ccaagcaatt
     3361 tagtaatgcc attttcttga atgaatctga gcaagaagat gccaaaaaat taaaagctct
     3421 tggcattaaa caatttattc aacaggtacc aacttctaaa aaagaagatt tgaatacaat
     3481 gttataaagg aggaatgaaa atgctcgttc agtcaattgg tttagggtta atcggtgttt
     3541 tttgtatttt agactcaagg cttttaggaa gaatgaattt tgaacgaccg ttgattgtca
     3601 gtacgcttgt tggtttggta cttggagaca tggaaaaagg attaattgtt ggagcctctt
     3661 tggaattgat gtccttagga ttagtaaata tcggagctgc ttcaccacct gatatgaata
     3721 tggcatcaat tattgctaca gcctttgcca ttttatcaaa tgcgaatgca gaagcagcat
     3781 taacgattgc cattccaatt tcagtgttag gtcaaatact aggtatctta atgcggacga
     3841 ttttgtcaaa tttgactcac gttgcggatc gctttatcga acaaggaaaa tttagccaag
     3901 caaggagaat tcatatactt tatggctcgg gattatatgc tctcatgtat tttattccta
     3961 tttttctagc aatttatttt ggaacgaatc ttgtttctca gattgttgat gcgattcctg
     4021 agtggctaac aaatggctta acggttgcaa gtaaaatttt accagcatat gggtttgcgt
     4081 tgttattaca aacgatgtta acgaaaaaaa tggtgccatt tttattactt ggatttttaa
     4141 ttactgctta ttctggattg ggaatcattg gtattgcagc cttcgcatgt attgttgcgt
     4201 ttgtcatggt tcaagttcaa ggtaaaaact caaatggtaa ccaaaatgag ccagatccat
     4261 tagatgactt ataaggaggt agattaagtg aaagagaaag atcaaaatgc atggaaatat
     4321 cggcagttct tatggcgttc atgggcaatt caagcctcat ggaactatga acgacaaatg
     4381 aacatgggtt ttatgtacgg aattgcacca attctagata acatttacaa gaatccagaa
     4441 gatgaagcaa tgaagaaaga agcttacgag cggcatatga tgtactacaa ctgtacccct
     4501 caaacaagtg catttgtgtt agggttaact gcttctatgg aggagcaata ctacgaagac
     4561 caagaaaact ttaatccgga atcaatcagt gcagtgaaga cttctctgat gggaccgcta
     4621 tctggtgttg gtgattcttt ctttcaagga acgattcggg tattagcctt tggactagga
     4681 attaatttag cccaacaagg tagtatttta ggtccaattc tagcaatgtt aatttccttt
     4741 gtaccttcct ttttagttac ttactatggt gggaaaattg gctataacat ggggaataaa
     4801 tacttgtcta aactgtatca agaaggtttg atggataaag taatgtatgt ttgctccatt
     4861 gtggggttga tggtcattgg ttcgatgatt gccagcatga tcgggattac aacaccaatt
     4921 caaatgggta aagcttttgt cttacaagat gtattagata gtattatgcc acaattttta
     4981 ccattaggat taaccttctt catgtactgg ttattgcaga aaaaggtcaa aacaggatgg
     5041 atgttggcaa tctgtttagt cggaggaatt atttttagtt tgttaggtat cttacaataa
     5101 gggaataacc aataaatttg gttgaataat ataaataagg aggaattcaa ttgatgaatt
     5161 caaaagaaat tgttaaagaa atcgtagcga aaaaagaggt aaaatcaatc gtatttgttg
     5221 gctgtggcgc ttcaaaagcg gacctttatc ctgcaaagta ttttctagac caaaatgcga
     5281 agcagttacg gattagtcat tacacagcca gcgaatttaa ctatgcaaca ccagcatctg
     5341 tagatgagtc aactgtcgtt atttcagctt ctttaggagg tacgactcca gaaacagttg
     5401 aagctaacgc agtagctaaa gaaaaaggtg ctacagttat tagtctaaca cgaagcattg
     5461 actcaccgtt aacaaaagat gcagattatg taatttatca tcgatttgca gagaactatg
     5521 ctgctaaatt agaaaaaaca ggctactgtt tagaattggc agtagaactt ttacatcaag
     5581 tagaaggtta tgaccattat gaagcaatgc aagaaggatt tgctactatc tatgatcttg
     5641 cccaaaaaag tgcagaatca gctcgtaaag atgcgaagaa atttgctgaa cgctttaaag
     5701 atgaaaaagt tatttatgta atgagtagtg gtgctacaca tgaagtagca tactcaacat
     5761 caatttgctt gatgatggaa atgcaatgga ttaattctgg taccttccat tcaggagaat
     5821 ttttccacgg accattcgaa attgtggata aagacgtacc gtttatatta ttaatgaatg
     5881 atggaaaaac tagaaatata gatgctcgag cattaacttt ccttgaacgc tttgatacga
     5941 aaacagaagt tgtagatgcc ttggattatg gcttgtcttc acatattgct aaagaagtct
     6001 tagactactt taacccattc gtgattactg cagtatttag agtttatgca gaagaattgt
     6061 cagaagcaag agaacatcca ttaactaaga gacgttatat gtggaaacta acttattaaa
     6121 aaatagatca aactaaataa ggtggcttga gaaattaagc caccttgttt tcttcaatgt
     6181 gattttatac atatggtaaa ataactagta catatggtaa aataactagt acatattgtg
     6241 aaagcaggtg gcaagatgca gcgattaaat gactatcttt atcaactaac attgcttttg
     6301 aaatctacgc cttcagataa ttattcagct cgcagtattt cgcaagctct acagttgaat
     6361 agaagtacaa tcagtagcta cttaaatgaa ggtgttcgtg aagggctttt cataaaagta
     6421 aaaagttacc cggttttatt tttacataga acagctcttg aagaactaca tatcacactg
     6481 aataactccg aaatagaatc aattgaaagc ttgttaacaa tttctcaaaa acccgcatta
     6541 gatcaagtaa ttggctctaa aggaagtttg aaagaagcaa ttgatcaaat taaaacggca
     6601 gtcttatatc ctggaaaagg gttgccttta ttattgatcg gcgctagtgg atcagggaaa
     6661 acctttctag cgaataaaat atatgaatat gcagtagaag aaaaggtcat tcacacatcg
     6721 gcacctttca tagattataa ttgtgcacaa tatgtgagca atccagagtt actatcctct
     6781 gctttatttg gctacacgaa aggagcattt accggagcaa gccaggagca tactggtttg
     6841 ctagaaaaag ccgatggagg agtattgttt ttagatgaag tgcatcgctt atctgaagaa
     6901 gggcaagaga aattatttac atttatggat acgggtgaat tctctcctat gggggataat
     6961 agtattcgga aaaaagccga tgttcgatta gtctttgcaa caacagaaaa tatatatacg
     7021 actttcttac caacttttct taggagattg ccagttattg ttaatctacc tagatttcaa
     7081 caacgtccgt cgtttgaacg tctgtcattg attgatgaat tttttgtttc tgagagccaa
     7141 attttagcta aggaactatc tgtttctgat gcattgattc atttcttaat gaatagtgat
     7201 tttgaaggta acgtggggaa tattaaaaat attatcaagt atgcttgcgg aaacgcatat
     7261 atccaccaga agagtcagca aacaattcaa gttaaattat tggatatgcc tttagagtac
     7321 agttataaat tcaaggagca actaaataaa cccagaaaga aaagagcaga tcgaatctac
     7381 tatccagaag aaacacgaca agtgcaacta gaaagaaaca atcagctttt tgttcaattt
     7441 tttgaccaat tacttactgg attctctgaa gtgattgaaa ataacaagga tattaaacta
     7501 tttttagaag aaatggttac caaggtaaca caagtaatgg atacgcttat ttttcaagag
     7561 gattacgaaa aagagcagtc tttatacgcc attctaaact atcatattcg tcaaagtatt
     7621 gattttatgc gagaaagcta cgggtttgaa caagatggta accgggtagt cgctttggcc
     7681 aactatctat atgtcaagga taatcaagtg atttttgaaa atagtcttca agaaaagaaa
     7741 accaaagatc agcttaacga attttttgct aatgttcttg aaacccctta ttggtatgca
     7801 aaaaaaatct taagcctttt atctgagtgt ttagatcaac ctgtgtatga agaggacatt
     7861 ttctttatta cgctatattt ttatagtttg aatgtcacga atgttaaaac aggtattaag
     7921 agtattgtgc tagctcatgg ttattctaca gctagtagta tggcgaatgt tgccaaccgt
     7981 atgctgaata agaacgtttt tcaagcttat gatatgcctt tagatattac attagagaaa
     8041 attgaagcaa aattgattca attccttgga gattatcgaa cagattcagg cttgatttta
     8101 ttggtagata tgggttcact aaaccaacta ggcggtttat tagtgaatca tctaaatgga
     8161 ccactgttaa tcattgatca tatcaataca ccgttattat tggaagtcgg ccaacaagta
     8221 ttaaacaata aaagtatcac cgaaatagga gcagaaattg aaacaagtct tactttcaaa
     8281 aagcagttac tacttcccaa gaaaaagaaa aagaaagcca tcattacttg ttgttataca
     8341 gggattggta gtgcgaccca gattcaggag attttaacga aatgcttgaa agatgcagtt
     8401 agagaattag taattattcc gtatgattac aaaaagctgt tagaaaacaa gaattttgag
     8461 actccttttc aattatacga tgtcatcatg attgtaggta cagaagatcc caaaattaat
     8521 cagattccat atattggatt agatcgcctg attaatgggg gaagcattgg cgaatttgtt
     8581 cattacttgg aaaaagagct gactattgaa gtgaacgatt tacaaaaaga attaattttc
     8641 aatttttcaa ttagtaaaat tgtcggaaat ttaaccattt tggatccaga taaggtgcta
     8701 tcttttgtac aaaaagcaat taaagaatta tcgaagttat taaattttga tttttctaat
     8761 aatcagctgt ttttgttgta ccttcactgt agttgtatga ttgaacgaat tgtacgtaaa
     8821 gaaagtgtgg atgagcaaga ggacattgaa gaatatatgc aagcaaatgg agaaataatg
     8881 gataaaattc atagcgcttt ttttgagatt gaaagggaat acacaattga aataccagat
     8941 ttagagttgc gattattgag cgatattgtt caaggatagg agtgaattcg aatgacaatg
     9001 ggagtaattt ttgatatgga tggggtactc atagatagcg agacatttta ttttgagaga
     9061 agaatgaact tctttcaaga actggagata gatccaggat caaaccagat tgatgattat
     9121 gtggggaaaa cagaagctgc aatatgggaa acattagttc ctgaggacaa atcattaaga
     9181 gaggaacttt atccgaaata tgtagattat cgaaaaacgc atccaattga tttcgctaaa
     9241 gctttacgaa aagaagtgaa accgttactt gacttcctca agaagcggga tataccattg
     9301 gcactggcat cttcttctcc agctgtcgag atagagagta tgctcattca atgtgaactt
     9361 actgaatatt tttcctttgt tataagtggt gagtcattaa aacaaagcaa accacatccc
     9421 gaaatatatt taaaagcgaa acagcaatta gactgtgact tctttattgc agtagaggat
     9481 tcaacgattg gtattaaatc agcaaaggca gcaaatatct atacggcggc actaaaacaa
     9541 ccaatgcaaa tcgatcagag tgaagcagac atacaaattg aaagtctaca agaactggaa
     9601 caaattgttt tagaaaaagg ttagtgaatt aatggaaata aggctatata tctgtgctag
     9661 ctattttgac taaccaaaaa aacttgctat aattcaggtg tagtaaaagg tgctggtaac
     9721 accttttacg aaaatgcttc ggcagtgata atgggattgg ctttttaagt tttttatttt
     9781 gttcgcaatc cctaaggtca tgtgaatgat cctagggatt gctagaatat tttgcaacag
     9841 aaccaaaata cgaatagtta tcaagcagcc atattgactg cggtcaattt aggaggggat
     9901 actgatacta ctgctgccgt aactggtgct cttgcgggta ttctttacgg gggtgaagtg
     9961 atccctgaaa attggagaaa atcgcttcaa aatgaccagc ttcttaaaac gatcagtcag
    10021 agattttgcg ataccgtgct atctcaaaga atttgaataa tggatatgat gcaaatcttg
    10081 tttgtactaa cgttgtattg acaaaaagga ttttatttag tacaatgtta gtacaaatga
    10141 tgaacggagg tagaatcatg tctcaatcaa ccattacatt taggattccc gacgatgaaa
    10201 aagaactggt attagaatat gcaaaagcac acaattcttc attgaccgaa ctttatcgaa
    10261 atgctgtact ggataaaatc gaagatgaaa tcgatctacg gactttacgg agtgcaattg
    10321 aagtttccaa agagaaaaaa gagagtggta tttctcaaga ggaaatggag aaactgctaa
    10381 atgaattata aagtcatcta catgccgcaa gcccagaagc aattaaaaaa aatggacaga
    10441 aaccaagcta aaataattgt cgcctggatc agaaagaatt tagaaaacac gactgacccg
    10501 cgtcagcacg gaaaaggttt gactggaaat aagagtgggg aatggcgcta tcgaattgga
    10561 aactatcgaa tattggctaa tatttgtgat tctgaaattg aaatccagat attctcaatt
    10621 ggacatagaa gtacaatata taaaagataa ccgtttttgg aacgaagtag agtttcgaaa
    10681 gtcaatgtat aatcttttga attttgcata ataccacagg tagaaactac ccgatactat
    10741 cggttggttt ctgctttttt
//
LOCUS       DACGLW010000094        10510 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.099, whole genome shotgun sequence.
ACCESSION   DACGLW010000094 DACGLW010000000
VERSION     DACGLW010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.099"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            complement(220..972)
                     /gene="xth"
                     /locus_tag="IXT53_002476"
     CDS             complement(220..972)
                     /gene="xth"
                     /locus_tag="IXT53_002476"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HAQ4906257.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            1085..2005
                     /locus_tag="IXT53_002477"
     CDS             1085..2005
                     /locus_tag="IXT53_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ4906258.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2078..3013
                     /gene="murB"
                     /locus_tag="IXT53_002478"
     CDS             2078..3013
                     /gene="murB"
                     /locus_tag="IXT53_002478"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HAQ4906259.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            3311..4789
                     /locus_tag="IXT53_002479"
     CDS             3311..4789
                     /locus_tag="IXT53_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ4906260.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            5066..7126
                     /locus_tag="IXT53_002480"
     CDS             5066..7126
                     /locus_tag="IXT53_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906261.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            7209..7778
                     /locus_tag="IXT53_002481"
     CDS             7209..7778
                     /locus_tag="IXT53_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ4906262.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(7953..8759)
                     /locus_tag="IXT53_002482"
     CDS             complement(7953..8759)
                     /locus_tag="IXT53_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906263.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            8897..10411
                     /locus_tag="IXT53_002483"
     CDS             8897..10411
                     /locus_tag="IXT53_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HAQ4906264.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
BASE COUNT         3726 a         1752 c         2071 g         2961 t
ORIGIN      
        1 atctgcggta ataagcccaa ccaaacgaaa aatatgagga actatttttg tttggctgtt
       61 cttattactt gatgcaggac agctttttca caatctctcg ataaacagtg ttcaaaagcc
      121 agtttctcgg tattatgcca aatttatgca aaatgtggaa agccattttc acaaaatttt
      181 tcttaatact cgaaactaaa cgactttttc acaacctctt tatttcaaat ctaattctac
      241 tggacaatga tcactgccca taatgtccgt atggatcttt gcatcagcta gtcctggctc
      301 taaatctttg ctcgtcaaaa agtaatcgat ccgccaacca gcattgtttt tccttgaatt
      361 gaatcggtag ttccaccaag agtagacacc ttcctgggtt ggataaaaat aacggaatgt
      421 atcgataaac ccgttatcca acagtcgaga aagagccgct ctttcttctg gagtgaatcc
      481 agcatttttt tgattcgttt tccagttttt caaatcaatc ttttgatgag cgacatttag
      541 atccccacac acgatgactg gtttttgttt ttttagattt tccaaataat tataaaaggc
      601 ttcttcccat tctaatcggt aatctaaacg ttttaattcg ctttgagaat ttggcgtgta
      661 acaggttaca agaaagaaat cggaatattc caaagtaatc agacggcctt ctgtatcatg
      721 gatatcgata cccattccgt aagtggcatt caatgctggt tttttagcaa aaatcgctgt
      781 gccggaatag ccttttcgtt ccgcgtaatt ccaatattga taataacctg gaagatctaa
      841 atcaatttgt cctgcttgga gtttcgtttc ctgcagacag aaaaaatcgg cgtctaactc
      901 atgaaaaact tctaaaaaat ttttatttac gatggcacga agcccgttta cgttccaaga
      961 gataaatttc aataaaagca cactccttcg aattaatttc cttacctttg cgatactatt
     1021 gtacctcatt ttagtaaaaa atggtgtaag atgaaagtaa ctaaatgatc caggaggaaa
     1081 gtagatgaag attggtgtaa tcggtgtagg gaatatcgca gaaaaagcat atttaccaac
     1141 ttatgcgaaa aaacaaggaa cacttgattt ttattttgca acaagaaata aagaaacaaa
     1201 agaaagaatt caaaaagagt acgggttcca acatctatat gaaacgatcg atgagctttt
     1261 agaagaaaaa atcgaagcat gtatgatcca cgcagcaaca aaagtacatt atgaattagc
     1321 caaaaaatgt ctagaaaatg gtatccatgt ttatattgac aaaccactga gtgtccatct
     1381 aaatgaagtt cgtgaattgc aagaattagc tgataagaac caaataattt tgatgatcgg
     1441 tttcaaccgg cgctttgctc ctatggtaga agaattgaaa cgtatcccag ataagcggtt
     1501 gatccacctg caaaaaaatc gaatagcggc aaaagaaacg acagagttcg tcctttatga
     1561 tttgttttta catcttattg atacagctgt gtatttatta gatgaaccca ttttgagaac
     1621 aaaataccag ataagagaaa caaaggaatt tttggaatat gccattttac aattagaaac
     1681 acaaaatcaa acggctattt taacgatgga tttagcaagt ggcgctaata tagaaaaata
     1741 tcaagtaaca agtaaaaatg gtacttatga agtaaacgat ctgacccagc ttgtcattca
     1801 agatgctgat ggaaaaaaag tgaaagaatt tggcgactgg acaaacacgc tagtcaaacg
     1861 cgggtttgag ccaatggtag aggccttctt ttctcagatc cgtttaggta aatcggaaac
     1921 agaattgctt aaacaaaaag aagttgtaag aagtcatgaa ttatgtgagg aagtgcttaa
     1981 agatcactac cgtcatcaac tttgaacaag actggcaaga ttgtattaag cgtgatagaa
     2041 taatgaggac gtttgagaaa agaaaggaaa atcctcagtg aataaagaag aaatagttga
     2101 aagcttagcc acgattacta tattgtttga tgaaccattg atgaactata cgtttacgaa
     2161 aacaggtgga ccagctgatg tccttgcatt cccgaaaaaa caagaagaag taaagcaaat
     2221 catcgattat tgccgaatcc atgatatacc ttggatggta ttaggaaatg caagcaatct
     2281 gatcgttcaa gatggcggga ttcgcggtgt agtcatcatg ctgactgaaa tgaaacagat
     2341 tcatgtcaaa ggaacgatgg tcattgcgga agcaggtgcc tcactgattg atacgactta
     2401 tgcagctttg gcagaatcat tgactggatt tgaatttgct tgcggcatcc cgggaagtgt
     2461 cggtggagcg gtttatatga atgcaggtgc gtatggcgga gagatcaaag atgtttttgc
     2521 tgaagtggat cttttgcttg aagatggaac attgaaaact ttgacaaaag acgaaatggt
     2581 attttcttat cgtcacagta aagtccaaga attacgtgct atcgtattgg aagctcgatt
     2641 cagtttgcaa acaggggatt atgaagcaat caaagcaaga atggatgagt tgaccgaatt
     2701 gcgtcaatca aaacagccat tagaataccc ttcatgcgga agtgtcttta agcgcccagt
     2761 aggacactat acaggtcagc tgatccaaca agcaggattg caaggcctga aatggggcgg
     2821 tgcacaagtc tctgaaaaac atgctggatt catcgtcaat atcgatcatg cgacagcgac
     2881 agattatatt gaattgattg cgcacatcca agaagtaatc aaagaaaaat ttgatgtgtc
     2941 tttagaaaca gaagtgcgga tcattggaga aaaacaagca atatcagaaa caagcagaag
     3001 tttagccaat taataagtaa tggaacttaa aataagtaaa agagcaggac ggtggccgtc
     3061 ctgctctttt ttgtggttgt ttacgcaaat ggcgcaaatc ttgtacattt taacacaata
     3121 aaacaaaaaa tatagaatat ttcctcataa atttttccat tttttgcaat attctcaaaa
     3181 aaaagataaa aaatgagaat aactgatgaa aaaacaagca taatccatca taaaatcagt
     3241 ataggtgaaa agatgaactt tcatctgcat atcaagtaat tggtatgagg cattttttga
     3301 ggtgtaggat atgaagttgg attaccttat agaagaggaa gatagagaaa aactgctgct
     3361 agctagatat ttagaatttg agaaaaagaa ttatgtagtg ctgcctaagt tatatgaaac
     3421 attaggtttg tctaaattta aaacaagaaa ttatatcata gaattaaata aagaatttac
     3481 tgaattcaaa acaaagccag agatccaaat ttcagaagaa gcagagatcc atgtcgaacg
     3541 tctgaatttg acggatatca aaaatttaag aagaatctac tttcaacgat caaaaattgc
     3601 tcgactgtta acggagatcg tggattcaaa agtaacgatc gaaaaatttg cagagaatca
     3661 ttttttaagc atatcaagag cctatgtaaa aagaagagaa ttagtctctt ttcttgaaca
     3721 gcatcaaata aagttaaaga aaaacatatt agttggggaa gaaataaaca tacggaacgt
     3781 attatttggg gtatatttcg gggtctttaa tggtttttct atgccattta gagaaagcgt
     3841 catgaatgaa gcaaagagta tcgtgcaata ttttgaatat ctgttcaatt taaaactgtc
     3901 tgcaacaaaa cgaatcaaat tcaatttgct aaccactatt agtttgttga gggcctatgg
     3961 gaatcaacca atcggaaaac gtttcttttc agatgaatac tttaagagct cagaaggaat
     4021 gaagatcatc aaggatttat cacatatttc ttttcttcat atccctaaag agcagatcat
     4081 tgatgagatt tcttatatca tgttgttttt gatcacggaa gaagaatttg aattagatgg
     4141 cgcatttgga cttgcggaag ctcattttca tgatatccac atgattagtc aaaaaatgtc
     4201 agtggaattg attgatcaat taccattttc gattgaaatc tctgaagaag aaagactata
     4261 tatacgaaaa gaacttcgta tgggtatgat gcatgtcaat cgaaaaaatt ggatattcga
     4321 ctttgagatg actagcttta cgtctaaaaa gcagctacag tttttcttcg agacgtatcc
     4381 aatattcagt acagcaatcc aaaaggtcat acaagagaat aaacgctatt tcttgggaaa
     4441 agatgaacgt ttcatgattc gcttatttta tgattatctc tttttattgg tcggtatttg
     4501 tccggtatca tattttgaag caccgattca tgtctgtatt gatttttcgc aaggatctgc
     4561 ttataccgat tacattattt ctcaagtgaa aggatttaaa aattacaata ttgtgattga
     4621 gagtcgaatc acttctcgta cgaatattta tttatctgat tgtttacttg aaaaatttaa
     4681 taaagaacag ataatttgga aaaatccacc gactccagat gactgggaat attttggaaa
     4741 taccatcatc agagaaaaaa atagacattt aactgaaaaa atcagctagt atttatggat
     4801 ggcagctttg tctcacatat ttattagctg atttatgttt ttttcaaaga aaaggtaact
     4861 agatataact agatataact agatatatta cttaacaaaa ttaaaaagta cataagttat
     4921 tattccgatt ttcttgaaac aataaaaaat caaaaaaaat actctcctaa tagtccatct
     4981 tcttttgatt ttatttatat aatgaaatca tcttatgaaa attactactt catgtttaga
     5041 caagagtagg gaggactaag aataaatgtg ttcaaaaaga cgttcaaaac aactaaataa
     5101 acacgcttgt cgtttagata aggaacggtc gtgttctgaa aaacactacg ttaagtttat
     5161 gatgcgcacg atgctgatca gtttatctgt aggtgtctat atgcatgtac ccgttcaggc
     5221 aacgacacaa accatcaagg tcgtacgtac ctctgtttct gagaccactg attcccctat
     5281 cactattact catactttca ctgatgcaaa tggaaaacag ctacttcctg cattgacttc
     5341 ctataataaa gtcagttatc ctccaacgat aaaccactac tggttaattc ctaacaaaac
     5401 attatggaca tcggaaaccg gaagcagtgt gccgctttct atcttttccg atatgactga
     5461 tcctacaaaa ggtgatcagt taaaacagat cactgatatt ttgaatcatg ctttgacaga
     5521 tataaaggac ggtggaagcg cacaagtttc ttatgtatat gaaaaagatt attcacagtt
     5581 tgaaagaaag aaaatcgatg ttctggcagg gacaaaagtt tcgatatctg atctggtaga
     5641 aaggctgaag aacgcagatg gaacacaagg agatatgtcc aaagtaattt caaacaatgg
     5701 gaatatcgtg gatacgacta atacaggaac atctactatt tccttgtcct attttgatcc
     5761 aattgcttta aaaacaatga ctgcatctgc tgtgatccat gttcgtgctg atcaaacagc
     5821 tatcaaagga aaagatcttc acttaagaac aggagagagt tacgccccta aagatcttgt
     5881 tgaatatgta acaaaaagcg atggaacttc aggaaataaa gaagaaatca aaatgaacgg
     5941 gacagtagat actacaactc cgggagaata tcccgttgta ttaacttata tagactcgaa
     6001 aacatcaaat gtggcagaaa caacagctgt agtttatgta gaagcatctg ctgcttcatc
     6061 gactgtcatc actcatttta tagatagtca aacaaataaa gaacttcaag cagaaacaca
     6121 tatgagtagt gtaaatcgta ttcctgattt gcgcctacct gctgaatttt ctgatcgagt
     6181 gccaaatata gatttatcga cttatgaaca aaatgggaaa gtcacttcta tttctgattg
     6241 gatgcaagca aacggattag acagaaaaag tcactggtcg gagatccttg aagtacttca
     6301 ctcagaactg atgaataata ggaagcaatc aggaggggaa attaccttta cttatgttta
     6361 tataccagac aatcgttcat tagaaggtca tgatcttatt gttcatccaa acgaaaaaat
     6421 aaacaaagaa gagttgatcc aatcagcaac aaattcctta ggtaaacccg taccgttaaa
     6481 ggatgtatcg gttacaatca atgaagtacc attggaggat ggttatttga ccaaacaaac
     6541 aggtgatttt acgattatgt atagttatca tgacccgtat tcactccaag aactccatgc
     6601 tcagtcacta ttacatgttt taccattaaa caaacagata gatcaaaaag aacaaaaaag
     6661 acaagaagaa agcttgtcag aaaaaaaaca acaaatggct catgcagttt ctccaacagg
     6721 gaaagtaaaa caaacaagaa gcctagagtt agcaagaata tctagaaaaa tctcagaatc
     6781 taataaaacg ataaggcaag aagctcattt ggccccacaa gtttctggat tggaaaatga
     6841 aattttagag cctaaaataa aacaacaatc agaaaaagaa tttgttgcta aacagtcgga
     6901 agatcagaag gaatcagatg accagaagga aacgaagaaa aaacgcttgt caactattga
     6961 ttccaccgat gagcatctgc agccgcaagt accacttcct agtccgggaa cacccgcacc
     7021 tggtggaggt attccttctt ctgctttagg agatttcatg cgaggtatgg caggaacatg
     7081 ggtatacgct aatcgaaatg aatttgacgc agatgactgg ttctgattta tatctctatc
     7141 tgttatagtc aaataagtag ctgagtcata tgttatttgg cataatggag acgaggaggg
     7201 atagaaaaat gttagataaa acgttacctt acgcagaaat ctggatgaca agaccacttc
     7261 aacaaaatgt tccaatcacg cctttagcgg aaggttttga atttcgattt tatcaaaaag
     7321 aagatgaatc tgcttgggcg gagattgaga cttccgttgg ggaatttcaa acgacagatg
     7381 aagcacttga gtattttcaa caaaactttg ctccttatcc tgaagaactt gaaagaagga
     7441 tgttttttgt agagacaaaa aaaggagaaa aagtagcaac ctgtaccgct tggcgaaaaa
     7501 aatcaagaga gcaagtgtat ccggtttttc attggttagc agtgaaacca gattaccaag
     7561 gaaaagggct cgcaaaagca ttgactgcca gagtcttgca agaatttcct accctccata
     7621 cggacggacc aatctatctt cacacacaga catggagcca tcaggcgatt gctctttata
     7681 aaaaaatggg ttttactttt attgctgaaa atctagacgg cacacctaat ccagattatg
     7741 aaaaagttat gcaggtttta agtaaattag ataaataacg aaatggtgaa ataagtcctc
     7801 agtattggga taaaaaacga aatagtcatt cctcatatct tacttttttg agttaatact
     7861 atcggataac taatggcatg ccatacattc aaaaaagaag gggcagtgac aaaagtattg
     7921 tcactgcccc ttctcttgtt ttttagcatg ttttatcgtt tgattttttt aggaataaaa
     7981 ttcttgatga acgtaaattt ttggtaatcg tttcgactat agaggtaacc aaatacactg
     8041 aaaacaactg ctccaataaa attaacaaat aaatccttca tagtatccat gattcccacg
     8101 tctaagtaac cattttggat gattgtttta tctgttttac catctgcatt ttgactttca
     8161 atgattgtcc gagtgatatc tggaacatga tagacagtgt ttccgtcttt acttaactcc
     8221 acactattga tttgctggat gatcgtatct ttttgcatat cgaattgcag taatttatct
     8281 gctgaaaatt ccacaaactc ccacataaca ccaactgtca tcgaaaaaca gaaggaaaca
     8341 actgctagta ataaaggagt caagttcatg gtttttgtat ttttattgag caaataaaca
     8401 agggaaaagc cgacaccccc agctaaaaat ccgctgaatg catgtagaaa agtatcccac
     8461 attgaaaaat agccataaaa atcacttaac tctcctaaaa ctgttgaact aaaaataaaa
     8521 gataacaaaa tgatttctag catatttgga atctgtatct ttaatatctt ttccagaaaa
     8581 taagggattt caaataaaat taatgtcagt aataaaagca ttgtatttcc ccagtgttgg
     8641 ttccagacct gatccactag cataataacg acgatgatac gtaataaaat atagattccg
     8701 tttgtgattc tacctgcagt ttttgtactg ttcgtaaaaa aggttttcaa atgattcata
     8761 aaataactcc ttcatgtata tccttaatta tactaagaag cgtgtgttta ccaaagctat
     8821 atgacttaaa gaaaaacaga gttgatattg tgaaaaaaat cacttaatat agtataagga
     8881 gaaaaggggg atacaaatga aaaaattagt tgctggttta ttaacagccg ttgcacttct
     8941 tgctatgagc gcatgcggcg cagatcagaa aaatacagcg cagacaagtt ctagtacgaa
     9001 ggaatctgcc gaagtatcat caggcgcttc tgaacaagta tatacagatc caaaagaaat
     9061 gaaagaatct tatgatgtcg tgattattgg agctggtggt gcagggatgg cagcagctat
     9121 cgaagcaaaa gatgctgggt tgaatccagt aattttggaa aagatgcctg ttgccggagg
     9181 aaacacatct aaatcatcaa gcggtatgaa tgcttcagaa acaaaatttc aaaaagaaga
     9241 aggaatcaaa gactcgaatg atgccttcta tgaagaaacg ctaaaaggtg gtggaggaac
     9301 gaatgataaa gaaatgcttc gatactttgt cgatcactct gctgatgcgg tcgattggct
     9361 agatcaaaac ggtatcaaac tggacaactt aacgatcact ggagggatga gtgaaaagag
     9421 gacacatcgt ccgtctgatg gctcggctat tggtggatac ttagtggagg ggttattgaa
     9481 taacgtccat gagcgggaaa tccctatttt tgtcaacgca gatgtgacag acattaagaa
     9541 aaatggggaa acggtcaatg aagtgactgt acatgttcaa ggagaagaac caaaagaggt
     9601 caccggaaaa gcaatcgtag ttaccacagg cggttttggt gccagcaaag aatttattga
     9661 aaaatatcgt cccgatttag ctgattatat tacgacaaat caagaaggat caactggaga
     9721 tggtatcacc atgattgaaa aattaggtgg acaaaccgtt gatatggata aaatacaaat
     9781 tcatccaact gtgcagcagg atgagggggt cttgattggt gaggttgttc gcggagaagg
     9841 agcaatcttg gtcgatcaag aggggaaaag atttgtcaat gaaatggata caagggataa
     9901 agtatctgca gcaattacag cattgcctga aaaatcagct tatttgattt ttgaccaagg
     9961 cgtgcgtgat cgtgccacag ctattgaatt ttatgaccaa aaaggatatg tcacaactgg
    10021 aaagacaatg gaagaattag cagaaaaaat cgatttacca aaagaaacac tagcaaaaac
    10081 agttgatgga tggaatacgg atgttgcgga gaaaaaagat gctcaattca accgtgccac
    10141 agccatggaa catgacttat cgtctccgaa ttattatgcg atcaaaatcg caccaggaat
    10201 ccattacacg atgggcggtg tgaaaatcaa tacgaacact gaagtattag ataaagaaaa
    10261 ccagccaatt agggggttat atgctgctgg tgaagtcaca ggtggcttac atggaaacaa
    10321 ccggatcggt ggaaattcag ttgcggagat cattattttt gggcgacaag cgggcataca
    10381 agcagctcga tttgcttcgg aaaatgaatg aaatagaacg taaataaaaa gaggttgtga
    10441 aatcagcgtt ttgagctaat accgagggtc agctgattga acactgttta gtgaagaggt
    10501 tgtgaaaaag
//
LOCUS       DACGLW010000095        10022 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.100, whole genome shotgun sequence.
ACCESSION   DACGLW010000095 DACGLW010000000
VERSION     DACGLW010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10022
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.100"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            complement(<1..722)
                     /locus_tag="IXT53_002484"
     CDS             complement(<1..722)
                     /locus_tag="IXT53_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HAQ4906265.1"
                     /translation="MTYKNIVFDLDDTLYDHQLPFKRSVEKCFPTIDIQQIDNIYKRF
                     RCWSDVAFPKYTKKLISIEQLRIFRCQKTMEEFGIDSISRTEALDFQADYEYELDQIT
                     MIPEIHQLLLALHKNRIPIGILTNGPVDLQSRKLQNIGAYQYFEKQNIIISQSTHGGA
                     AENSYEGNVKNLQSYQNVQLSKRAIEILKEEIELNHQHIRFNPDYKDNGWIFTSKSRH
                     KPGMATVFLTNFIRCRTSFRML"
     gene            complement(727..1494)
                     /locus_tag="IXT53_002485"
     CDS             complement(727..1494)
                     /locus_tag="IXT53_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HAQ4906266.1"
                     /translation="MNKKRRLEKILDMLKIDGTITIKEIIDELDISDMTARRDLDALE
                     ADGLLTRTHGGAQLLSSKKPLEKTHIEKKSLNTKEKIDIAKKACSLIKDGDTIFIGPG
                     TTLEQLALELKGRKGYKIRVITNSLPVFLILNDSETIDLLLLGGEYREITGAFVGSMA
                     STNLKAIRFAKAFVSANAVTHNSIATYSDKEGVIQQLALNNAVEKFLLVDSTKFDRYD
                     FFNFYNLDQLDTIITDNQISPQHLEEFSQYTTILKAD"
     gene            1983..2408
                     /gene="lacA"
                     /locus_tag="IXT53_002486"
     CDS             1983..2408
                     /gene="lacA"
                     /locus_tag="IXT53_002486"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HAQ4906267.1"
                     /translation="MAIVVGADLKGTRLKDVVKNFLVEEGFEVIDVTKDGQDFVDVTL
                     AVASEVNKDEQNLGIVIDTYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     VGAEIVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMC"
     gene            2425..2940
                     /gene="lacB"
                     /locus_tag="IXT53_002487"
     CDS             2425..2940
                     /gene="lacB"
                     /locus_tag="IXT53_002487"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HAQ4906268.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGHEVLDFGTYDHARTHYPI
                     YGKKVGEAVVSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSAIYAKEELNAN
                     VIGFGGKITGGLLMNDIIEAFIEAEYKPTEENKKLIAKIEHVETHNAHQADEDFFTEF
                     LERWDRGDYHD"
     gene            2951..3883
                     /gene="lacC"
                     /locus_tag="IXT53_002488"
     CDS             2951..3883
                     /gene="lacC"
                     /locus_tag="IXT53_002488"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HAQ4906269.1"
                     /translation="MILTVTLNPSVDISYPLETLKIDTVNRVKDVSKTAGGKGLNVTR
                     VLYESGDKVTATGFLGGKIGEFIESELEQSPVSPAFYKISGNTRNCIAILHEGNQTEI
                     LEQGPTISHEEAEGFLDHYSNLIKQSEVVTISGSLPSGLPNDYYEKLIQLASDEGVAV
                     VLDCSGAPLETVLKSSAKPTAIKPNNEELSQLLGKEVTKDIEELKDVLKESLFSGIEW
                     IVVSLGRNGAFAKHGDVFYKVDIPDIPVVNPVGSGDSTVAGIASALNSKKSDADLLKH
                     AMTLGMLNAQETMTGHVNMTNYETLNSQIGVKEV"
     gene            3886..4866
                     /gene="lacD"
                     /locus_tag="IXT53_002489"
     CDS             3886..4866
                     /gene="lacD"
                     /locus_tag="IXT53_002489"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HAQ4906270.1"
                     /translation="MVLTEQKRKSLEKISDKNGVISALAFDQRGALKRLMAQYQDTEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDKEAGLLLAYEKTGYDTSSTK
                     RLPDCLDVWSAKRIKEQGADAVKFLLYYDVDSSDELNQQKQAYIERVGSECVAEDIPF
                     FLEILAYDEEISDAGSVEYAKVKPRKVIEAMKVFSDPRFNIDVLKVEVPVNVKYVEGF
                     ADGEVVYSKAEAADFFKAQEEATNLPYIYLSAGVSAKLFQETLQFAHDSGAKFNGVLC
                     GRATWAGSVEPYIKEGEKAAREWLRTTGFENIDELNKVLVKTASPWTDKV"
     gene            4894..5211
                     /locus_tag="IXT53_002490"
     CDS             4894..5211
                     /locus_tag="IXT53_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011669100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HAQ4906271.1"
                     /translation="MNREEMTLLGFEIVAYAGDARSKLLEALKAAENGDFAKADSLVV
                     EAGSCIAEAHNSQTAMLAREASGEELPYSVTMMHGQDHLMTTVLLKDVIHHLIELYKR
                     GAK"
     gene            5211..6917
                     /locus_tag="IXT53_002491"
     CDS             5211..6917
                     /locus_tag="IXT53_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ4906272.1"
                     /translation="MHKLIELIEKGKPFFEKISRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAYVPNAWGFHWSKDIETFLMTPYSYSMGILAFFVGGTTAKALTDSMNRDLPATNQ
                     INFLSTMLASMVGFLLMAAEPAKEGGFLTAFTGTKGLLTAFIAAFVTVNVYKVCVKNN
                     VTIRMPEEVPPNISQVFKDLIPFTVAVVLLYGFELIVKGTLGVTVAESIGTLLAPLFS
                     AADGYLGITLIFGAYAFFWFVGIHGPSIVEPAIAAITYANIDANLHLIQAGQHADKVI
                     TSGTQMFIVTMGGTGATLIVPFLFMWICKSERNRAIGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFVPFIFAPIVNVWIFKFFVDTLNMNSFSANLPWVTPGPLGIVLGTNFQVLSF
                     ILAGLLVVVDTIIYYPFVKVYDEQILEEERSGKTNDALKEKVAANFNTAKADAVLGKA
                     GVAKEDVAANNNITKETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGSYGAHR
                     EMLPEFDLVILAPQVASNFDDMKAETDKLGIKLAKTEGAQYIKLTRDGQGALAFVQQQ
                     FD"
     gene            7059..8465
                     /gene="lacG"
                     /locus_tag="IXT53_002492"
     CDS             7059..8465
                     /gene="lacG"
                     /locus_tag="IXT53_002492"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HAQ4906273.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYHKYPVDLELAEKYGVNGIRISIAWSRIFPTGYGEVNEKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEVLHSDGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKDKGYKGEIGVVHAL
                     PTKYPYDPENPADVRAAELEDIIHNKFILDATYLGHYSDKTMEGVNHILAENGGELDL
                     RDEDFQALEAAKDLNDFLGINYYMSDWMQAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     IAPDYVPRTDWDWIIYPEGLYDQIMRLKNDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKKHLEVLSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFDTQERY
                     PKKSAHWYKKVAETQVIE"
     gene            8488..8761
                     /locus_tag="IXT53_002493"
                     /pseudo
     CDS             8488..8761
                     /locus_tag="IXT53_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209209.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
     gene            8782..9681
                     /locus_tag="IXT53_002494"
     CDS             8782..9681
                     /locus_tag="IXT53_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002889672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HAQ4906274.1"
                     /translation="MTIELKNEYLTVQFKTLGGQLTSIKDEDGIEYLWQADPNYWNGQ
                     APILFPICGSLRNDWAIYRPQERPFFTGLIRRHGFVRKEEFILEEVNDNSVTFSIKPN
                     AEMLDNYLYQFELRVVYTLNGKSIRTEFQVTNLEMEKTMPYFIGAHPAFNSSLVEGEN
                     YEDYYIEFSEVESCSIPKSFPETGLLDLQDRTPFLENQKILDLDYSLFSHDAITLDQL
                     KSRSVTLRSRKSGKGLRVDFDDFPNLILWSTTNKSPFIALEPWSGLSTSLEEGNFLED
                     KRQVTKISPQETSRKIYDITILN"
BASE COUNT         3165 a         1690 c         2005 g         3162 t
ORIGIN      
        1 aatagcatac ggaaagaagt tctgcacctt ataaaatttg tcaaaaaaac tgttgccatt
       61 ccaggtttat gacgactttt ggaagtaaaa atccatccat tatctttata atcaggattg
      121 aatcttatat gttggtgatt taactcaatt tcctctttaa gaatttctat agccctttta
      181 gataattgga cattttggta actttgtaag ttttttacat taccttcata gctattttct
      241 gcagctcctc cgtgagtgga ctgactaatg atgatatttt gcttttcaaa atattgatag
      301 gcaccaatat tttggagttt tcgactctgt aagtcaacag ggccattagt tagaatacct
      361 attgggatgc ggtttttatg tagagccagc agtagttgat gtatctcagg aatcatcgta
      421 atctgatcga gttcatattc atagtcagct tggaagtcta gagcttctgt cctagaaata
      481 ctatcgatgc caaattcttc catagtcttc tgacagcgaa aaattctaag ctgctcaata
      541 ctgatgagtt ttttcgtgta tttaggaaaa gcaacatcag accaacatct gaaacgtttg
      601 taaatgttat ctatttgttg gatatcaatc gttggaaaac atttctcaac gctacgttta
      661 aaggggagtt gatggtcata aagagtgtca tcaaggtcaa aaacaatatt tttataagtc
      721 ataattctag tccgctttta aaatagtagt gtactggcta aattcctcta agtgttgagg
      781 gctaatctgg ttatctgtaa tgatggtatc gagttgatct agattataga agttaaagaa
      841 atcgtatcga tcgaatttag tactgtctac taataagaat ttttctacag cattgtttag
      901 ggcaagttgt tgaatcacac cttccttgtc actatatgta gcaatagaat tatgggtaac
      961 agcatttgca ctaacaaaag ctttggcaaa tctaattgct tttaaatttg tcgaagccat
     1021 tgaacctaca aaagctccag ttatttctct atattcaccg ccaagaagca ataaatcaat
     1081 ggtttcgcta tcatttagaa tcaagaacac agggagacta tttgtaatga cacgaatttt
     1141 ataaccttta cgacctttca attctaatgc cagttgttca agtgtagttc cgggtccaat
     1201 aaaaatagta tcgccatctt tgattaaaga gcaggctttt ttagcaatgt caattttttc
     1261 ttttgtattt agacttttct tctcgatatg tgtcttttca agtggctttt tagaggaaag
     1321 taattgtgca ccaccatgag tacgtgttaa aagtccatca gcttctagag catcaaggtc
     1381 tctacgggct gtcatatcgg aaatatctag ttcatctatt atttctttta tggttatggt
     1441 cccatcaatc tttaacatat ctaaaatttt ttctaatcgt cgttttttgt tcatatgaag
     1501 acccttcttt cataaagtaa tttttttcca aagataattc tcttttaatt gtatcataaa
     1561 agacaatatt ttcaaggtaa aacaaacaat ttcaaacaaa aacaaatgtt agatgatgaa
     1621 ataagaacag tggattgacg tatattagct taggtcagat tttgtataag acgaaaataa
     1681 agtaggacct cttaatcagt aagttataga aagtaaaaga cttttgtaat acttgaatag
     1741 atatttcacg tccattttgt gatggattaa atgaacaaaa atgaacaata atttaacggt
     1801 gttatctatt ttttaagaaa acaaataaaa aaaacaaaaa ataaacaaaa aagtgttgct
     1861 ttttgtttga atgattgata ttatataaac aaagaaatga tgaaaacgtt ttttcgaaca
     1921 ttttgcaaaa tgttttctac ttctacgtag catttctttc taaaatttag gaggtggtca
     1981 aaatggctat tgttgttggt gcagatctca aaggtacgag attaaaagat gttgtgaaaa
     2041 attttcttgt agaagaaggt tttgaggtaa ttgatgtgac taaggacgga caagattttg
     2101 ttgatgttac cttggcagtt gcctctgaag taaataaaga tgagcaaaac ttaggtattg
     2161 taattgatac ttatggagct ggtccattca tggtagcaac taaaattaaa ggtatggttg
     2221 cagcagaagt ttcagatgaa cgctcagctt atatgacacg tggacataac aattcacgaa
     2281 tgattacggt tggcgctgaa atcgtcggtg atgagcttgc taaaaacatc gctaaaggtt
     2341 tcgtcaatgg taaatatgat ggcggacgtc atcaaatccg agtagatatg cttaacaaaa
     2401 tgtgttaaga aaaggagtta aggaatgaga atcgcaattg gatgtgacca cattgtcaca
     2461 gatgaaaaaa tggcagtatc agaattcttg aaatcaaaag gacatgaggt ccttgatttc
     2521 gggacatatg accacgcacg tactcactac cctatctatg gtaaaaaagt cggtgaagca
     2581 gtagtaagtg gtcaagcaga cttgggagta tgtatctgtg gtacaggtgt cggcattaac
     2641 aatgcagtca ataaagttcc aggtgttcgc tcagcgttgg ttcgtgatat gacatcagct
     2701 atatatgcaa aagaagagtt aaatgcgaat gttatcggtt tcggtggaaa gattactggt
     2761 ggtcttctta tgaatgacat cattgaagct ttcattgaag ctgagtacaa accaacagaa
     2821 gaaaataaaa aattgattgc aaaaatcgaa cacgttgaaa cacataatgc acatcaagca
     2881 gatgaagatt tctttacaga attccttgaa agatgggacc gtggtgacta ccacgattaa
     2941 ggtataacaa atgattctga cagtcacact aaatccttca gtagacatct cttatcctct
     3001 agaaacattg aaaattgata cagtgaatag ggtgaaagat gttagtaaga cagctggtgg
     3061 aaaaggctta aatgttacta gagtcttgta tgaatctggt gataaagtta ctgctacagg
     3121 tttcttaggt ggtaaaattg gtgaatttat tgaaagcgaa ttagaacaat ctcctgttag
     3181 tccagctttt tataaaatct caggaaacac tagaaactgt attgctatct tacacgaggg
     3241 taaccaaaca gaaattttgg aacaaggacc aacaatttct catgaagaag cagaaggctt
     3301 tcttgaccac tatagcaatc ttattaaaca atcagaagtt gtgacaattt caggaagttt
     3361 accttcagga cttcccaacg attattatga aaaacttatc caacttgctt cggatgaagg
     3421 agtagcagtc gttttggatt gttcaggtgc acctcttgaa acagttctaa aatcgtcggc
     3481 taaacctact gccatcaaac ctaataatga ggaactttca cagttgcttg gaaaagaagt
     3541 aacaaaagat attgaagaac tcaaagacgt tcttaaagag tcacttttta gtggtatcga
     3601 atggatagtg gtctcattag gtcgaaacgg tgcctttgca aaacatggtg atgttttcta
     3661 taaggtagat attcctgata tcccagttgt taatccagtc ggatcagggg actcaacggt
     3721 agctggtatt gcatcagctt taaatagtaa aaaaagtgac gctgacttat taaaacatgc
     3781 gatgacattg ggtatgttaa atgctcaaga aacaatgaca gggcatgtga atatgactaa
     3841 ttacgaaaca ctaaactcac aaattggagt aaaagaggta taaaaatggt acttacagaa
     3901 cagaaacgca aatctttgga aaaaatttca gataaaaacg gtgttatctc agctttggca
     3961 tttgaccaac gtggtgcttt gaaacgtttg atggcacagt atcaagatac agaaccaact
     4021 gtagctcaaa tggaagaact aaaggttttg gttgctgatg aacttacaaa atacgcttct
     4081 tcaatgttgc ttgaccctga atatggactt ccagcaacaa aagcattgga taaagaagca
     4141 ggtcttcttc ttgcctatga aaaaacaggt tatgatacat caagtacaaa acgtttgcct
     4201 gactgcttgg atgtttggtc tgcaaaacgt atcaaagagc aaggcgctga tgccgttaag
     4261 ttcttgcttt actatgatgt agacagctca gatgaactta accaacaaaa acaagcttat
     4321 attgaacgtg ttggttctga atgtgtcgct gaagatattc ctttcttctt ggaaatcctt
     4381 gcttatgatg aagaaatctc agatgcaggt tctgttgaat atgcaaaagt gaaacctcgc
     4441 aaagtcattg aagcgatgaa agttttctct gatccacgct ttaacattga tgttcttaaa
     4501 gtagaagtac cagttaacgt taaatatgtc gaaggttttg ctgatggtga agtggtttac
     4561 agtaaagctg aagctgctga cttctttaaa gcacaagaag aagcgactaa ccttccatac
     4621 atctacctaa gtgcaggtgt atctgctaaa ttgttccaag aaacattgca atttgctcac
     4681 gactcaggtg ccaagtttaa tggtgtgctt tgtggacgtg ctacttgggc aggatctgta
     4741 gaaccttaca tcaaagaagg tgaaaaagca gcacgtgaat ggttgcgtac tactggattc
     4801 gaaaatattg atgaacttaa caaagttctt gttaaaacag ctagtccatg gactgataaa
     4861 gtatagtaaa aacataaaac ggaggatatt gttatgaaca gagaagagat gactctctta
     4921 gggtttgaaa ttgttgctta tgctggagat gctcgctcta aacttttaga agcgcttaaa
     4981 gcggctgaaa atggtgattt cgctaaggca gatagtcttg tagtagaagc aggaagctgt
     5041 attgcagagg ctcacaattc tcagacagct atgttggctc gagaagcttc tggggaggaa
     5101 cttccataca gcgttaccat gatgcatggt caggaccact taatgactac tgtcttatta
     5161 aaagatgtga ttcatcacct catcgaactt tataaaagag gagcaaaata atgcataaac
     5221 tcattgaact tattgagaaa gggaaaccgt tctttgagaa aatctctcga aacatctatc
     5281 ttcgtgctat tcgtgatgga tttatcgctg gtatgccagt catccttttc tcatctatct
     5341 ttatccttat tgcctatgtc ccaaatgctt ggggattcca ctggtcaaaa gacattgaga
     5401 ccttcttgat gactccttat agctattcga tgggtatcct agcattcttt gttggtggta
     5461 ctaccgctaa agctttgacg gactcaatga accgtgacct acctgcgacc aatcagatta
     5521 acttcttatc tacgatgcta gcgtctatgg tagggttcct tttgatggca gctgagcctg
     5581 caaaagaagg aggcttcttg acagccttca cgggaacaaa aggtcttttg acagcattta
     5641 ttgcagcctt tgtgacagtt aatgtttaca aggtctgtgt gaaaaataat gttactattc
     5701 gtatgcctga agaggttcca ccaaatatct ctcaagtatt taaagacttg attccattca
     5761 ctgtagcggt tgttctcctt tatggatttg aactcattgt taagggaact cttggtgtaa
     5821 ccgttgcaga atcaatcggt acccttcttg ctcctctctt ctcagctgca gatggttacc
     5881 ttggaattac acttatcttt ggtgcttatg ccttcttctg gtttgttggt atccacggtc
     5941 cttcaattgt cgaaccagca atcgctgcta tcacttatgc caatatcgat gccaacttgc
     6001 atcttatcca agctggacaa catgcagata aagttatcac ttctggtact caaatgttta
     6061 ttgttaccat gggtggaaca ggagctacat tgattgttcc attcttgttc atgtggattt
     6121 gtaaatcaga acgtaaccgt gccatcggac gtgcctcagt tgttccaaca ttctttgggg
     6181 ttaatgagcc aatcttattt ggtgcaccaa tcgtattgaa tccgattttc tttgtaccgt
     6241 tcattttcgc tccgattgtc aacgtttgga tctttaaatt ctttgttgac acattgaaca
     6301 tgaactcatt ctctgccaac cttccatggg ttactcctgg tccgttggga attgtactcg
     6361 gtactaactt ccaagttcta tcatttatct tagccggact cttggtagtt gttgatacta
     6421 tcatttatta cccatttgtt aaggtatacg atgaacaaat tcttgaagaa gaacgttctg
     6481 gtaaaacaaa cgatgctctt aaagaaaaag tagctgcaaa cttcaatact gctaaagcag
     6541 atgccgttct tggaaaagca ggcgttgcta aagaagatgt agcagcaaac aataatatca
     6601 caaaagaaac taatgtcctt gttctttgtg caggtggagg tacaagtggc ttgcttgcca
     6661 atgccttgaa caaagcagca gcagaataca atgttccagt caaagcggca gcgggtagtt
     6721 acggtgctca ccgtgaaatg ttgccagagt ttgacttggt tatcttggct ccgcaggtcg
     6781 cttcaaactt cgatgatatg aaggctgaaa cggataaatt gggcattaaa cttgcaaaaa
     6841 ctgaaggagc tcaatatatc aaattgacac gtgatggaca aggtgctctt gcatttgttc
     6901 aacaacagtt tgattaataa atttgtaaaa gttatttgag acgagattat tctttcagtt
     6961 ccttataata ttgttaacca tatcattgca tttcatttcc ttggtttccg gaactcagaa
     7021 tatcgtctca aatgcttttt ttgaaaggat ttatagttat gactaaaaca cttcctaaag
     7081 attttatttt tggtggtgct acagctgctt atcaagcaga aggtgcgact cacacggatg
     7141 gtaaaggccc agttgcatgg gataagtatc ttgaagacaa ttattggtac acagcagagc
     7201 ctgcaagtga tttttaccat aaatatcctg ttgatttaga attagctgaa aaatatggcg
     7261 ttaatggtat tcgtatttca attgcctggt ctcgtatttt cccaactggc tatggcgaag
     7321 taaatgaaaa aggtgttgaa ttctaccata atctttttgc cgagtgccac aaacgtcatg
     7381 ttgagccatt cgtgactttg caccactttg acacaccaga agttcttcac tcagatggag
     7441 atttcttaaa ccgcgaaaat atagaacact ttgtagatta tgccgctttc tgttttgaag
     7501 agttcccaga agtaaactac tggacaactt tcaatgaaat tggcccaatt ggagatggtc
     7561 aatacttggt tggtaaattc cctccaggca ttcaatatga tcttgccaaa gtcttccaat
     7621 cacaccacaa tatgatggtt tctcatgcac gtgctgtgaa attgtataaa gataaaggtt
     7681 ataaaggcga aattggtgta gtacatgcgt tgccaaccaa atacccttat gatccagaaa
     7741 atccagcaga tgttcgtgcc gctgagttag aagatatcat ccataacaaa tttatcttgg
     7801 atgcaaccta ccttggacac tattcagata aaacgatgga aggggtcaac cacatcctag
     7861 ctgaaaatgg tggagaactt gatcttcgtg atgaagactt ccaagcactt gaagcagcta
     7921 aagatttgaa cgatttcctt ggtatcaact actacatgag tgattggatg caagcctttg
     7981 atggtgagac tgaaatcatt cacaatggta agggtgaaaa aggaagttct aagtaccaga
     8041 ttaaaggtgt tggtcgccga atcgcaccag attatgtgcc tagaacggat tgggactgga
     8101 ttatttatcc tgaaggcttg tatgaccaaa tcatgcgatt gaaaaatgat tatccaaact
     8161 acaagaagat ttacatcact gaaaacggtc ttggatacaa agatgagttt gtagataata
     8221 ccgtttatga tgatggtcgt attgattacg tgaagaaaca cttggaagtt ttatcagacg
     8281 caattgcgga tggtgcaaat gttaaaggtt acttcatctg gtcacttatg gatgtcttct
     8341 catggtcaaa cggatacgaa aaacgttatg gactattcta tgtagacttt gacacgcaag
     8401 aacgctatcc taagaaatca gctcattggt ataagaaagt agcagaaact caagtgatag
     8461 agtaatatta cttgaaaatg taaaaaattg ttatccgata ctttagaaaa agtacaacac
     8521 ttatttagac caggctttga aaaggtatat attgttagtt tcgaagattg tcctatgatt
     8581 cctgagcttg aagctaccct cgttgctaaa atgtggtaaa tggtatgttt taactggaaa
     8641 agagtggatt tgccattctg atttagagtt atcagcattt gaatgggaat ttttacaatc
     8701 actcgatgta gaaatacgtg agactattca cttcgaggta aattatttgc ctttccaata
     8761 ataaaaagaa aggaattagt tgtgactatc gaattaaaaa acgagtatct tactgttcag
     8821 tttaaaacac ttggtggtca actgacttcg attaaagacg aggatgggat agagtatctc
     8881 tggcaagctg atccaaatta ttggaatgga caggcgccta ttctatttcc tatctgtggt
     8941 agtttacgaa atgattgggc tatttataga cctcaagaaa gacctttttt tacaggtctt
     9001 attcgtagac atggttttgt tcgaaaagaa gaatttattc tggaagaagt caatgataat
     9061 agcgtgacct ttagtattaa accaaatgct gagatgcttg ataattacct atatcagttt
     9121 gaactgagag ttgtttatac cttgaatggt aaatcaatta gaaccgaatt tcaggtaacg
     9181 aacttggaaa tggaaaaaac aatgccttac tttattggag cccatccagc attcaatagt
     9241 tctttagtag aaggtgaaaa ctatgaggat tattatattg aatttagtga ggttgagtct
     9301 tgctcgatac caaagagttt tcctgagaca ggattattag atttgcaaga tcggacacct
     9361 tttttggaaa atcaaaaaat tctcgatttg gactactcac ttttttccca tgatgccatt
     9421 acacttgacc agctcaagtc tcgtagcgta acgcttcggt caaggaagtc tggtaaaggt
     9481 cttcgagtag attttgatga ttttccgaac ttgattctgt ggtctacaac taataaaagt
     9541 ccattcattg ctttggaacc ttggagtggt ctctccactt cacttgaaga aggtaatttc
     9601 ttagaagata aaagacaggt aacgaaaatt tcacctcaag aaacttctag aaaaatctat
     9661 gatattacta tccttaatta aaatttgtta ttatttgagt cttcaataag atttgaatac
     9721 tcttaccttg tgatgataga ttggtcttct ccgtgccagt cttacatcgc aaggttttta
     9781 agttttttgt gggtctatgt caactgtagt gggtgacgaa aagctaatat ctaaagacag
     9841 tctggtagta aaaaataaga ttatttacta cttcttgatt taaaataata gtggagccat
     9901 taatttgtgg caaatttaat ttaatatttt gcctcaaatt agccacactt tttatagaac
     9961 taagaggaaa aatgaaattt tcttcctatt aatatagttg tttgaaatct taaatatagc
    10021 gt
//
LOCUS       DACGLW010000096         9951 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.101, whole genome shotgun sequence.
ACCESSION   DACGLW010000096 DACGLW010000000
VERSION     DACGLW010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9951
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.101"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            complement(<1..1817)
                     /locus_tag="IXT53_002495"
     CDS             complement(<1..1817)
                     /locus_tag="IXT53_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906275.1"
                     /translation="MKIVKEKIASRKIHWLALSILVLMFIVVFTGNVVQASIFNWFDD
                     TESQTEFLNNPVYQSYLKKYNGNFYSQFGLWIGWSVIKSMFAIVDGIQNMIPEVLDLF
                     SFIESTGMNKVYQSVLSTVVIGLMILSLMFVGYKMITGKGTVDLKSVGMNVVMSVALI
                     LLMPTLIGSTEKLSGIMFAKTLYNDATSINNQDGGVAWSLIEQGVTDLAYINKTDQYS
                     TIDKTNDRNNLTKENFWKTDLTQVLTDNVIDKMEKENPTADNLRYELVENSDNEFVAT
                     KFSDNFLSTFVDGIKSGYYRYQANLWGISFGLIALAVAYVFSAFIIITAILELAFKRV
                     LGVLVFATDLETGQRSKQVLSDILQCYLTVGFQGFGLSMFAMFINYLNTGEGISANIF
                     IKTIAYICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGTGGMNRS
                     KGGKNNGGNDQAEGEERKPERNFGQSLSKKANKTGKALGYAHERGLSGLASDGSTMAS
                     ERATKPFKSMRDIANDTKSKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSMPT
                     RLSDRKDGAKVENADSIMSSSERMREAMKNNAESNNNPV"
     gene            complement(1804..2022)
                     /locus_tag="IXT53_002496"
     CDS             complement(1804..2022)
                     /locus_tag="IXT53_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906276.1"
                     /translation="MIENRLIDQVWCLDQNTDNLELIETICFNTIVLDLRSENDNCVT
                     HIIIDRKTAQKLSESLRKWAEGGNNENS"
     gene            complement(2098..3111)
                     /locus_tag="IXT53_002497"
     CDS             complement(2098..3111)
                     /locus_tag="IXT53_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HAQ4906277.1"
                     /translation="MEEIDKLIAQYAYSKDDVYAEKYLKYPIFLDKHKKYKKMKQSTK
                     KVYMYLKKQNEYSLENSKVDSKGRIFLIFAIEKIAEKSCLTKKTVISALRELEEYKLI
                     TIKKNGFNKKTGKNNPNFYYLLKPEFSEGDFYKKKCIEENLHLPTNQHYSPSEKNTPS
                     DKSTFDGDKSFPLPGEKFLQELNHSSKDFKDFKESQNADFIDSLSVENKKLDEILIES
                     LIEKEWLYQVWGDNIVNCLKLVGNNDYSQFYDWYKKFKYAIKSAEKEQKIQLKIIYEI
                     CNNKYFVDYVREQLSMTLRDLIRRAKTDHRIKQKDDYIFASLKNKALELIELQISEGI
                     DKQ"
     gene            complement(3315..3596)
                     /locus_tag="IXT53_002498"
     CDS             complement(3315..3596)
                     /locus_tag="IXT53_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ4906278.1"
                     /translation="MKSEIQVILPSDLDQELRRYFYRVAKEAVDQVRIETSLSKEVFR
                     LGEACKYLSCSRSTLKKFIREKGLKVSIIGDCQFIRKEELARFIKEYEV"
     gene            complement(3647..3853)
                     /locus_tag="IXT53_002499"
     CDS             complement(3647..3853)
                     /locus_tag="IXT53_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ4906279.1"
                     /translation="MKPKDFRKFCGLSQSELAEKYGISVQAVRNKESGKTNYSPEEMI
                     ILKYLVKEKGIENITIDQIFFNEK"
     gene            4017..4748
                     /locus_tag="IXT53_002500"
     CDS             4017..4748
                     /locus_tag="IXT53_002500"
                     /inference="COORDINATES: protein motif:HMM:NF024252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ4906280.1"
                     /translation="MFDKNINSEIGKRIQEIRVSLGYTLEEFGENLTPPASKSNVSRW
                     EKGKNIPNKARLLSIAKLGGVSTEYLLTGNSQKTKKYIKYLFNEIFFDNGTFHNKFKD
                     FGKDRDRLGLYDLFLAVSKTYSLDKSPSLKDNFVEMIIDDVYKKLLQTDKSDDQLILG
                     FIYICLDEKLSNRQKSLKSINYEVFNYLGVAFGLIDSEIKSTNAGNDEDLLNYLKELT
                     SFIDEIKTTTFNKKKYFQNQYDIEE"
     gene            4802..5947
                     /locus_tag="IXT53_002501"
     CDS             4802..5947
                     /locus_tag="IXT53_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HAQ4906281.1"
                     /translation="MATIKQYEKKDGTKAWMFQTYLGISPLTGKEVRTTRRNFKTKKE
                     AQIELNRLLVDFEENGLKRETNETFQEVYDLWYESYKTTVKEVTQLKIEIKFNKWILP
                     KYGQFRIKEITTKHVQKILNQWAKTTDQYKVLHSTVSRIFKYAMTLGLITKNPCELVI
                     MPNRNVEKKKDTVKIYSRDQLETLFQYLESKRSTYRAEYDKTLLRFLFFSGCRISEAL
                     ALDWSDIDFDSNTVTINKTLSQTKYGYKISSPKTRNSYGSLSLDNKTINWLKKWQINQ
                     KRYMLTLGITDPTMIFCGIYKEIITKHAIYSRMITICKNSGIPFLGNHVTRHTHASML
                     LESGVSMKEVQERLRHSSIKLTMDTYAHLTKETKEKTVEKLAEHLNF"
     gene            complement(6032..6337)
                     /locus_tag="IXT53_002502"
     CDS             complement(6032..6337)
                     /locus_tag="IXT53_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HAQ4906282.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(6362..6793)
                     /gene="ruvX"
                     /locus_tag="IXT53_002503"
     CDS             complement(6362..6793)
                     /gene="ruvX"
                     /locus_tag="IXT53_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HAQ4906283.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(6790..7056)
                     /locus_tag="IXT53_002504"
     CDS             complement(6790..7056)
                     /locus_tag="IXT53_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HAQ4906284.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(7311..8036)
                     /locus_tag="IXT53_002505"
     CDS             complement(7311..8036)
                     /locus_tag="IXT53_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HAQ4906285.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(8397..8467)
                     /locus_tag="IXT53_002506"
     tRNA            complement(8397..8467)
                     /locus_tag="IXT53_002506"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8433..8435),aa:Gly,seq:ccc)
     gene            8584..9336
                     /locus_tag="IXT53_002507"
     CDS             8584..9336
                     /locus_tag="IXT53_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HAQ4906286.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            9352..9831
                     /locus_tag="IXT53_002508"
     CDS             9352..9831
                     /locus_tag="IXT53_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HAQ4906287.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
BASE COUNT         3088 a         1907 c         1529 g         3427 t
ORIGIN      
        1 cttacaggat tattattcga ttcagcatta tttttcattg cttcacgcat acgttcagaa
       61 ctagacataa tactatcggc attttcaacc ttagcaccat cttttctatc cgaaagacgt
      121 gttggcatgg aatcagcata ttttccagtt tcatcttctt tatttttagc taacataggt
      181 ttgctatttt tattaatagc ggacacagta ccatcatcta aaccttcttt aaacttactc
      241 ttggtatcat tagctatatc acgcatactc ttaaatggtt ttgttgctct ttcactagcc
      301 attgtagatc catctgaagc taaacctgat aatccacgtt catgagcata cccaagcgct
      361 ttccccgttt tattagcttt tttagaaagt gattgtccaa agttgcgttc aggttttcgt
      421 tcttcccctt cagcttgatc gtttccacca ttatttttcc cacctttaga ccggttcata
      481 ccacctgttc ctttacggaa tagcattgca cccattccaa aagcagaagc aagttgccca
      541 tagccttctt tgagtccaat atccacacca aagtatctca tgactgttcc actgcctttg
      601 attaaaacaa acacagcaca gatataagca attgttttta taaaaatatt tgctgagata
      661 ccttcgccag tatttaggta atttatgaac attgcaaaca tagataaacc gaacccttga
      721 aatccgactg tcaaatagca ctgtaaaata tctgacaaaa cttgttttga tcgttgtcct
      781 gtttctaagt cagtagcaaa tactaaaact ccgagtacac gtttaaatgc taactcgagt
      841 attgctgtaa ttataatgaa agcactaaaa acatacgcta ccgctaaagc aatcaatcca
      901 aaagaaattc cccacaggtt cgcttgatag cgataatatc ctgatttgat accatcaaca
      961 aaggttgata aaaaattatc tgaaaattta gtagcaacaa attcattatc agaattttca
     1021 actaattcat aacgcagatt atcagctgtt gggttctctt tttccatttt atcaattaca
     1081 ttatctgtca atacttgcgt aagatccgtt ttccagaaat tttcttttgt tagattattt
     1141 cggtcatttg ttttatcaat tgtcgaatat tgatctgtct tattaatata agctaaatct
     1201 gtcactccct gctcaatcaa tgaccatgct acaccgccat cttgattatt gatactagtt
     1261 gcgtcattat aaagagtctt tgcaaacatt attcctgaaa gtttttctgt tgatccaatc
     1321 aaagtcggca ttaaaagaat taatgctaca gacatcacta cgttcattcc aaccgatttt
     1381 aaatccactg ttccttttcc agtaatcatt ttgtaaccaa caaacatcag tgaaagtatc
     1441 attaaaccaa taacaactgt acttaagacc gattgataaa ctttattcat tcccgtactt
     1501 tcaataaagc taaacaaatc taacacttcg ggaatcatat tttgaatacc atctacaatt
     1561 gcaaacatcg attttataac agaccaccct atccacaatc caaactgact gtaaaaattt
     1621 ccgttatatt tttttaaata cgattgatag acaggattat tcaaaaattc agtttgactt
     1681 tccgtgtcat caaaccaatt gaaaatgctt gcttggacaa cattaccggt gaaaacaacg
     1741 ataaacatta aaacaagtat gctcaaagct aaccagtgta tttttctact ggcaatcttt
     1801 tctttaacta ttttcattat tacctccctc agcccatttt ctgagactct cagagagctt
     1861 ctgagctgtt tttctgtcaa ttatgatatg agttacacaa ttgtcatttt cagatcttaa
     1921 atcgagaact atagtgttga agcaaatagt ctcgattaat tcgagattat ctgtattttg
     1981 atctagacac caaacttgat ctattaaacg attttcaatc aaaataaccc ctcctatatt
     2041 caaagttctc aaatgaagcg gagatacccc tccacttcat ttgactcaat tctattctta
     2101 ttgcttatct attccttcac tgatttgcaa ttcaattagt tctaacgctt tattttttag
     2161 agaagcaaag atataatcgt ctttttgttt gatacggtga tctgtttttg ctcttcgtat
     2221 caagtcacgt aaagtcattg atagctgttc tctaacataa tctacaaaat acttgttatt
     2281 acagatttca tagataattt tcagttgaat tttttgttct ttttcagcag atttaatcgc
     2341 atatttgaat tttttatacc agtcataaaa ttgactgtaa tcgttattac ccactaattt
     2401 cagacagttt acaatattat ccccccatac ctgataaagc cattcttttt caattaatga
     2461 ttcaattaat atttcatcta attttttatt ttcaactgaa agtgaatcaa taaagtcagc
     2521 attttgagat tctttaaaat ctttaaaatc tttagaagaa tgattaagtt cttgtaaaaa
     2581 tttttcacct ggaagcggga atgacttatc accatcgaat gttgatttgt cggaaggtgt
     2641 atttttttca cttggactgt aatgttgatt tgtcggaagg tgtaaatttt cctcaatgca
     2701 cttttttttg tagaaatctc cttcgctgaa ttctggtttc aataaataat aaaaatttgg
     2761 attattcttc ccagtttttt tgttaaatcc atttttttta atcgtaatta atttatattc
     2821 ttctagctct ctcaaagcgc ttattaccgt tttctttgtt aaacaagatt tttcagctat
     2881 tttttctata gcaaaaatca aaaaaattct tcctttgctg tcaacttttg aattctctaa
     2941 agaatattca ttttgttttt tcaaatacat ataaactttt tttgtacttt gtttcatctt
     3001 cttatatttt ttatgtttat ctaaaaaaat cggatacttc aagtattttt cagcatatac
     3061 gtcatctttt gagtatgcat actgagcaat taatttatct atttcttcca tttattcctc
     3121 ttctttctaa taaatgttaa aatgtagtca aataaagttt tcttagaact ttattgtcga
     3181 gtgtttcctg tggtcgccaa actgtcggga acactccttt taattttttt caaagtattt
     3241 ttatattttc tatacttcat cattattcaa acaaacactt ccttttttct atcaaaagca
     3301 cgccacgcaa ctttttatac ttcatattct ttgataaacc ttgctaattc ttcttttcta
     3361 ataaattgac aatctccgat tatgcttact tttaaacctt tctctctgat aaattttttt
     3421 aatgtacttc tgctacagct taaatactta caagcttccc ccaatctaaa aacttctttg
     3481 cttagagatg tttcaattcg aacttgatct actgcttctt ttgcaactcg atagaaatat
     3541 cttcgcaatt cttgatctaa atccgatgga agaataactt gaatctcaga tttcattttc
     3601 tttgccccca ctaatttgac tacatgtatt caaattcaac ttttcgtcac ttttcattaa
     3661 aaaaaatttg atcaattgtg atattttcaa ttcctttttc tttcacaaga tacttcaaaa
     3721 taatcatttc ttctggagaa tagtttgttt taccgctttc tttgtttctt accgcttgta
     3781 ctgaaatgcc atatttttca gcaagttcag attgtgataa tccacaaaac tttctaaaat
     3841 ctttaggctt catgactcct cactcctcct tcttttcaac ttttcgtcac taaaagaata
     3901 acatatattt attcataccg caacgtaaaa ttcaactttt atctactttt tcatttcatt
     3961 atatgagaat gtagtgtaaa ctgtagaata gaatagatga aggtggtgaa accattttgt
     4021 ttgataaaaa cattaattct gaaattggaa aacgaataca agaaatacgt gtttctttag
     4081 ggtatactct tgaagaattt ggcgaaaatc tgacacctcc cgcaagcaaa agcaatgttt
     4141 ctagatggga aaaaggaaag aacataccca acaaagcgcg gctactaagt attgctaaac
     4201 ttggtggtgt ttctactgaa tatctactga caggtaattc tcaaaaaact aagaaatata
     4261 tcaagtattt attcaatgag attttttttg acaacggtac cttccataac aaattcaaag
     4321 atttcggtaa agacagagat aggcttggtc tatatgactt atttttagca gtatcgaaaa
     4381 catatagtct agataaatca ccttctttaa aagataactt tgtagaaatg attatagatg
     4441 atgtatataa aaaactcttg caaactgata aatcagatga ccaactcatc ctgggtttca
     4501 tatatatttg tcttgatgaa aaactttcaa acagacagaa aagtcttaaa agtatcaatt
     4561 atgaggtatt caattattta ggtgttgcat ttggtttaat tgatagcgaa atcaaatcta
     4621 caaacgcagg taacgatgaa gatttattaa attatttaaa agaattaaca tcttttattg
     4681 atgaaataaa aacaactaca ttcaacaaaa aaaaatattt tcaaaatcaa tatgatatag
     4741 aagaataaag attacatttt cctatcaaaa gcacgccacg caactgatag gaggaaaaac
     4801 aatggcaaca attaaacaat atgaaaagaa agacggaacc aaagcttgga tgtttcaaac
     4861 ataccttgga atcagcccgc taactggtaa agaagtccgt actacacgta gaaattttaa
     4921 aacaaaaaaa gaagctcaaa tagagcttaa tagactactt gtagactttg aagagaatgg
     4981 acttaaaagg gaaacgaacg aaactttcca agaagtgtat gatctctggt atgaaagcta
     5041 taaaactact gtaaaagaag tcactcaatt aaaaattgag atcaagttta ataaatggat
     5101 tcttccaaaa tatgggcagt ttagaattaa agaaattact acaaaacatg ttcagaagat
     5161 attgaatcag tgggctaaaa caacagatca atataaagtc ctacattcta ccgtaagccg
     5221 tattttcaaa tacgcaatga ctctagggct gatcactaaa aatccctgtg agctcgtaat
     5281 catgcccaac agaaatgttg aaaaaaagaa ggatactgtc aaaatttatt cacgagatca
     5341 attagaaacc ttatttcaat atttggaatc caaaagaagt acgtataggg cagagtacga
     5401 taaaacttta ctgcgctttc tattttttag tggctgtcga atcagtgagg ctttagcctt
     5461 agactggtca gatattgact ttgattcaaa caccgtcact ataaacaaaa ccttaagcca
     5521 aacaaaatat gggtacaaaa tttcaagtcc aaaaacgaga aatagctatg gttccctatc
     5581 tttagacaat aaaaccatta attggctaaa gaaatggcaa atcaatcaaa aaagatatat
     5641 gttaaccctc gggattactg atccaacaat gattttttgt ggaatctaca aagaaatcat
     5701 tactaaacac gctatctatt ctcgtatgat cacaatctgc aagaattctg gtattccatt
     5761 tctaggtaac catgtaacca gacacactca tgctagtatg ctgttagagt cgggagtctc
     5821 catgaaggaa gttcaagaac gccttagaca ctcatcaatc aaattaacaa tggataccta
     5881 tgctcatcta actaaagaaa ccaaagaaaa aaccgttgaa aagcttgcag agcacctaaa
     5941 tttctagcgg aacgtattca atcccgtatt caattcaaaa caaaaaaaag ctgaaccctt
     6001 atctaccaag ggttcagcta tcatttacag cttattcttc ttcttccgcc atgaatgtat
     6061 tgaatacttc ttcaatcatg tcccattctg cgtctgtttc gatttgttct aactcgcctt
     6121 ctgttccatc ttcgttttcg atgtatgcat aagcttgtaa ttctacatct tcatcttctg
     6181 aaacaccagc aggatataaa agaacatagt tacgtccaaa ttcttcttgt ccgtcaactg
     6241 ttagtaggat ttcatataat gtttcatttc cttggtcatc caccaatgtg atgtgttcgt
     6301 gtccttcatg gtcgtgggaa tggttatgtt ctgtcataag attacctctt ttcttttatt
     6361 attaaaataa ttgcttacct gagccgtcta aataattttg taagatcatc acagctgcta
     6421 atttatcgat gacttttttt cgtttggctc ttgaagtatt tgcctgctct actagcatac
     6481 gttctgcctg aacagtggtt aatcgttcat cttgataatc aactggcaag gagaatagtt
     6541 ctttgatctt ttctccgtat gacatggaag cttctgccct tggtccgatg gaattgttca
     6601 tatttttagg taatccaact acaaaccggt ctacttcata ttgtgctact aattccttca
     6661 accgatcaaa gccaaactct tcttctgctt cattgatccg aatgatctct actccttgag
     6721 ctgtccagcc aaaggggtca ctgatagcaa cacctactgt cttagaaccg acatccaagc
     6781 ccattactct cataaattca ctccatgatt tgagaggtag tccttgacaa ttacttccat
     6841 gatctcatcg cgttcgtgac ggcgaatcaa atttcgagca tcacgataac gaggaatgta
     6901 ggccgggtct ccagaaagca agtagccgac aatctgattg attggattgt agcctttttc
     6961 ttccaaagcc cgataaacaa tcgctaatgt ttcgctcact tcttttttgc gactatcgtc
     7021 aaaaccaaaa cgtacggttt catctgtaaa acccatgatt ctacacctct ttcccgcaag
     7081 gtaattccag cttaccttct acaaccttat tttactcgaa gaaagggaaa actacaaaca
     7141 aatattccaa acttccgaat aaattaagaa acactatttg tatgtttttt tccttttttc
     7201 tgtagcttta tcaaaagtac tcttttcata aagtatacgc tatatttctt tgaatgtatg
     7261 tttttacaca attttttcat aatattcata aaaaattcga atagctgcta ctaatttttt
     7321 gtttttgttg cttgtcctgt ctcataattg atcgtgtagc catcttgaac attgtagata
     7381 aagacattga attcaatttt tttgtcttca atgctttctg cttccaattg cactcccctg
     7441 gctaccagtt cattcccctc aaaatgagga gtgacacggt agcgtacatg atgatttgtt
     7501 tctttgatat atgccgcaat atcattttcg tgagcaagca tgtatggagc attcaacgaa
     7561 cgtgtccctg tcatcaagtt tttaatgttg ttattttgac cagtcagctg gaaaccaatt
     7621 aaatggctgc gattatataa ccattgcccg tcacttaatt tcttttgttt ccaacctgtc
     7681 gggtcaatat acaaaggctc tctcttttca gtagggaaca aatcttttcc aagcatagca
     7741 ttggcttgtc ctactctgtt caatttatca atatttgaat agctttccca tgttcctttg
     7801 gaaaggctaa gatcctcttt tgtaaattcc ggctgattcc cattgatttc tatttcttga
     7861 tgttttccat catattctgg cacatccgct acttgagaag cttgtggtgt ggaatcaaaa
     7921 atagttgtaa tctgctgaaa gatatctgaa tctttttgtg tggtggaaac accgaatgcc
     7981 cctgcaatca cagcagcgac gatccccacg atcgccttgg ttgttttttt gctcatttct
     8041 ttactccttt actaacagct ccctaaatct taacatatgt tcccctataa ataaatacta
     8101 gaaagaaata gaaacagcaa aaaataaaac ctgttttaag atggcttaaa aatacaaaaa
     8161 agagacggat tcctcgtctc taatcagtca ccactctatc tgtaaggtat ttattattat
     8221 tcaaaaattt tcttcatact caagacaata cactgatttc acaaccgctt ggtaaacggt
     8281 gcgcaaaagc ttgcatctgc ggtaataagt ccatccaagt taaaaacata agaagttgtt
     8341 tttgcttggc tgttcttatt acttgttgca gagcagcttt ttcacaacct attttaagcg
     8401 ggtgacggga atcgaacccg cgacatcagc ttgggaagct gggattttac cactaaacta
     8461 cacccgcaat acaaaaacag tatacgcctc ttttattcct tcctcaagtt tttttattct
     8521 ttacgcttct ggtatgtttt gtagtaaaat aaaactaatc aatcaattat tggagaagat
     8581 gctatgacgg gaaaagaatt agatctgttg ctggacactt gtttactcgc tgggaaaatc
     8641 atgatggaaa gcaatgcgga gatgtatcgg gtagaagata cgatgagtcg aattgcttta
     8701 gcttctggaa atttccgttt ggtcagctat gtcactcaaa ccggtctctt tattggtctt
     8761 gatcgtacct cgaccatccg aatggaacaa attaccaacc gttcgattaa tttagaaaaa
     8821 gtagtaaaag taaatgatct ttcacgtaaa tatgttgctt cagagctaac attagaagaa
     8881 ctttactgcg aactgcaagc aatcgaccga gaccgttctt tctttcctgt ctggttgcaa
     8941 atcgttagcg cggcagtgat cagtggaaca attatggtgt tatttggcgg aaagatgacc
     9001 gatttacctg tgactttagt cattggagga ttgggatacg cagcttatct atatagtttg
     9061 aaatttttta gaatcaaatt tttatcggaa tttttatctt ctttattgat tggcatagcc
     9121 gctatctaca gcgtacgttt tggcttagga agcaatcagg atctaatcat tatcggatgt
     9181 gtgatgcctt tagttcctgg agttcaaatt acgaacgcta tccgtgattt gctagctgga
     9241 cattatgtct caggagtttc tcgcggaaca gaagcgatga tgactgctac gatgatcggt
     9301 tttgcgattg cttttatttt ccagcttttt tattaggaaa ggaacacaag tatggggacg
     9361 atatttattc agttttcttt tagtttttta gcgactgcgg catttgccgt tattacaaat
     9421 gtacctaggc gttcattgat ttgttgtggt ctttctggtg cttttggctg gatgatctac
     9481 tggatctgtg ttcaaatagg cggatctgct gcttttggtt cattgcttgg ttctttagga
     9541 gtagcattca tcagcgatct tttctctaaa cgtttgaaaa tgcccgtaac gattttcaat
     9601 attcccggga tggttcctct agtccctggt ggtttagctt atcaagcggt tcgaaatctc
     9661 gttactgaat cctatcgaga agcggctcat tatgcggtcc aagcaattat gattgctgga
     9721 gcaatcgctt taggtcttgt attatcagaa gtctttaacc ataacatccg taatttcaaa
     9781 gtaaagcgtg acgcaatcat gatgaaacga aaaaaacggc tttttaaata aacgaagtgt
     9841 aacaaagaaa aggttgtgaa agagacgttc agccttgagc gatgacaaaa tatctcacaa
     9901 acattgcgac aagtaaccgc aggagcaata agaagtaatt atgagtatta a
//
LOCUS       DACGLW010000097         8959 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.102, whole genome shotgun sequence.
ACCESSION   DACGLW010000097 DACGLW010000000
VERSION     DACGLW010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.102"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            complement(<1..360)
                     /locus_tag="IXT53_002509"
     CDS             complement(<1..360)
                     /locus_tag="IXT53_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAQ4906288.1"
                     /translation="MATVWMGLALVGNTFSPNSAMEGQGVIDVLTHIYTTGPAEYAKS
                     ILVNVFFGAFFGRVLVDSGIAATLIRKVVELGGDKPRITMSLLCIVTAVIFMSMTGIG
                     PVISIAVIVLPILMSLGI"
     gene            complement(712..1071)
                     /gene="rplT"
                     /locus_tag="IXT53_002510"
     CDS             complement(712..1071)
                     /gene="rplT"
                     /locus_tag="IXT53_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HAQ4906289.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(1124..1324)
                     /gene="rpmI"
                     /locus_tag="IXT53_002511"
     CDS             complement(1124..1324)
                     /gene="rpmI"
                     /locus_tag="IXT53_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HAQ4906290.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(1396..1920)
                     /gene="infC"
                     /locus_tag="IXT53_002512"
     CDS             complement(1396..1920)
                     /gene="infC"
                     /locus_tag="IXT53_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HAQ4906291.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     gene            2240..2872
                     /locus_tag="IXT53_002513"
     CDS             2240..2872
                     /locus_tag="IXT53_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HAQ4906292.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGNQSYLDAKN
                     MVLRESHIQKLTMQKTGLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTTIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(2874..3779)
                     /locus_tag="IXT53_002514"
     CDS             complement(2874..3779)
                     /locus_tag="IXT53_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HAQ4906293.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWEIALIISLVLSIWMLIALWRRIR"
     gene            complement(3864..4517)
                     /locus_tag="IXT53_002515"
     CDS             complement(3864..4517)
                     /locus_tag="IXT53_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ4906294.1"
                     /translation="MIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFYAN
                     PEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEVCV
                     YDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYDYY
                     KELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVSI"
     gene            4839..5783
                     /gene="mvk"
                     /locus_tag="IXT53_002516"
     CDS             4839..5783
                     /gene="mvk"
                     /locus_tag="IXT53_002516"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HAQ4906295.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            5776..6753
                     /gene="mvaD"
                     /locus_tag="IXT53_002517"
     CDS             5776..6753
                     /gene="mvaD"
                     /locus_tag="IXT53_002517"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HAQ4906296.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKKGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            6766..7851
                     /locus_tag="IXT53_002518"
     CDS             6766..7851
                     /locus_tag="IXT53_002518"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HAQ4906297.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            7848..8912
                     /locus_tag="IXT53_002519"
     CDS             7848..8912
                     /locus_tag="IXT53_002519"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HAQ4906298.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKDPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAQEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
BASE COUNT         2694 a         1859 c         1736 g         2670 t
ORIGIN      
        1 aatacctaat gacatcaaga tcggcaacac aataactgca atagaaatca ctggtccgat
       61 tcctgtcatc gacatgaata taacagcggt tacaatacat agtaagctca tcgtgattct
      121 tggcttgtcc cctccaagtt caactacctt tcgaatcagg gtagctgcga ttccgctatc
      181 tactagaaca cgcccgaaaa aggcaccaaa gaagacattt accaagatcg atttggcgta
      241 ctcagctggc cccgtcgtat agatatgcgt caacacatca attactcctt gcccttccat
      301 cgccgagttt ggtgaaaacg tgtttccgac tagcgctaat cccatccata cagtagccat
      361 gatgacaaaa gccgaccatc aagttgtagc cttttacaca gtaccagacc atgaaaaaga
      421 atgagataac taacaaaata ccaataataa tatcactcat gttattcctc cgtccatttt
      481 gtttttctga taagcaaact aatatatcag tcaaactcat tctaacttat gtagttagcg
      541 ctttcattaa aggatgacca taaatgttca gttttcaatt aaaaaacctt aattatgtta
      601 ttttagactt atacgttatt gtcctgtttc atttgatgga ggattgtaaa taaaaaaaga
      661 ggaaagaaca taacttttcg ttatcttctt tcctctttat aattgtttag cttatttagc
      721 caaagcttct tttgcttggt cagccaatgc agtaaatgct gctgcatcgt taacagccaa
      781 gtcagctaac attttacggt tgatgtcgat ttcagcaact ttcaaaccgt gcattaattt
      841 tgagtagctc aagccattca tacgagctgc tgcgttgata cgagcaatcc acaatttacg
      901 gaagtcgcgt ttcttttgac gacgatcgcg atatgcgtag ttgtatgaat tcattacttg
      961 ttcttttgct gttttaaata aagtgtgttt tgaaccgtag taaccttttg ctaattttag
     1021 cactctttta cgacgtttac gagttactgt tccaccttta acacgtgcca tgtataattc
     1081 ctcctaataa aaataaatct tcagattctg ttatttgtac ttcttatttc atttttgcta
     1141 gttgttggcg aatacgtttg aagtcgccac tagaaaccat gcttgcttta cgcaattgac
     1201 ggcgttgttt ttttgttttg ccgtggaaac ggtgacttgt aaacgcgcgg aagcgtttca
     1261 atccgccttt accagtacgt ttgaaacgtt ttgctgatcc gcggtgtgtt ttttgttttg
     1321 gcatgactat atttcctcct caaaatcttt cgttatccga ctatcggctg tccaacagcc
     1381 agttagtcat tcaaattact tactgtcgtt tttcggtgcc agcgtcaaga acatgctgcg
     1441 tccgtccatt ttcgcttttt gttccactgt tgcaatatcc gcagtttctt cagctaagcg
     1501 attcaagact ttttgaccaa tttctttatg ggtaatggca cggcctttga aacggataga
     1561 agctttcact ttatcgccct tttccaagaa cttacgtgca ttacgaagtt ttgtattgaa
     1621 gtcattcaag tcgatcgtag gacttaagcg aacttctttt acattgatca ctttttgttt
     1681 tttacgcgct tcgcggtctt tcttttgttg ttcgaaacgg tatttaccgt agtccatgat
     1741 tcgcgcaact ggtggtttcg caccaggggc aactaacaca agatccaagt ttgcttgttc
     1801 tgcaatattc aatgcttctg cttttgtctt aactcctaat tgctcaccat ctgaaccgat
     1861 caatcgtaac tctcgtgcac gaatgccgtc gttaaccatc atatcctttg ctatggtcat
     1921 tcacctccaa atattatgag agaaagaaaa gcttaccgaa taaacggcaa taaaaaaacg
     1981 agttctctta cagaacccgc gcgtacttca cctaatagaa ccagtgaaaa ctatctagcc
     2041 cagtgacatt atcaactaag gcgagaagcg ggtgcttctg cttttatttc tcaaccatta
     2101 tagtatagca aaaactaatc tttttgtcaa tgtttttcat acagtttgtc ttccttttct
     2161 gaaaatgatt agattagcag gctttctagc agtgattccc gcttctttgc tatactaaag
     2221 aaaaacgagg tgaacccaca tgaaaaaaat cgaacctatt ccaccttctc ttcctcttct
     2281 tacaaaggga gagttttatt tagaagatga ggagatcttc tctggtcttc tgatcaacgg
     2341 aggtaaccaa agctatctcg atgccaaaaa tatggtatta agagagagtc atatccaaaa
     2401 acttacgatg caaaaaacag gtttacgtca ttttgaatgc agtaatgtca tttttgaaaa
     2461 atgtgatttt tccaatctgg agtggattgg tggcagtttc caccaagtcg tgtttcatca
     2521 atgtaaatta acaggtacga attttgccga aagttatctt cgagactgta ctttcactga
     2581 ttgtattgct tcgatggctt cctttagtag tacaaacttg aaagctgtct ccttcgatca
     2641 ttgtcaatta gaagacagcg aattttatga ggtcacttgg aaaaaccttt ttttgtctga
     2701 caatcagtta accggttcga actggtttcg aacttctctg aagggattag actttacgac
     2761 taatttcttc acaacaatcg ctttatctca agattctttg gcgggactaa tcgtcaatca
     2821 agagcaagca cttgtgatcg cccaagccct tggtttgcag atcaaagact agatcaacga
     2881 atccgccgcc acagtgcaat aagcatccaa atagataaga ccaaactgat gatcaaggcg
     2941 atttcccatc cgaaaacgga atgggtaaag ggtaactgga gattcattcc gaagaatcct
     3001 gtcacaatag ttggaacggt caagatcagc gaccagaccg tcaaaaattt catcgtgtca
     3061 ttcaagttgt tattcagcaa attattataa gtgccagaga gctggtctaa aatctgcgaa
     3121 gctaagttcg tcatttcaac tgcttgtcgt gcttcgatca gtgcatcgtc caactcttct
     3181 ttttcctttt ctgacaactt gcgataaata gccagtgcct tgatttgttc taaaagtact
     3241 acattttgtt ttgttcctgt aactaagtaa accaaaccga tctctaaatc tgaaagttgc
     3301 agcagattct tgttcgttgt tttttctctt aatttcagat taagccgctg acgttcacta
     3361 ttcacttcct cgattaaagg aaaaaaggaa tcagaaatca aaaacaatgt acgaaacaat
     3421 aaactgtata cactcatgtc tggcatccgt tccaaaagcg atcgaattag tcgaatcacg
     3481 tactcggtat tcggcgtggt aaatgtaaac aaaccgtctt cttttaaaat aaaagcgata
     3541 ggactagttt cataatgatt atcgactttt tgctggtgcg gaacatttga tacaagcagt
     3601 aacgcttcat caaaagaatc gtactctacg cgcgcccgtt catttttatc tagagaataa
     3661 gtcagcatct cgtcactaat gccatattct tcttttaagt ttttaacagc tagcacatcg
     3721 ttttgctgta cattgatcca aaaattacgg tcttgattaa aatagtgttt ttcccgcata
     3781 ctctatcctc ctgacatttg tttccagtat aaagaaaaaa gtcgctcaaa taaaggttta
     3841 ccctttattt gagcaactgt aaatcaaatg gatactttgg tttggcgaat ctctgctaat
     3901 ttttcttcga tcattttcaa gacttcttct tttgcttgag gatcttccac aaaattatag
     3961 cggtctccat cgatttggat ttttggactt tctttgtatt cttcatacca ttgatcataa
     4021 cgtctgttca attctttgta ataatcgtat agacttgggt caaaggacaa ttgttcatac
     4081 gaacgaccac gtttttcgat acgctctaac atcgtatcaa aagatacacg aatatgaact
     4141 aataaatccg gatgtttttt atgggcagca tagggaagtt cttccatcat gttttccagc
     4201 agttcatcat acacacaaac ttctgtttct gttgctcttc ctagatctgc atttagatgg
     4261 aaaagcagtg aatcttcata aatcgaacgg tccaatacat tattgtcatc tttcattgct
     4321 tgtttgatgc ttgcaaaacg tttattcaaa aaataaattt gcagcaaaaa agcatactgt
     4381 tcagggtttg cataaaataa gggcaagact tcattatcat caatcgattc ataaaatgcc
     4441 tgactatcaa agtgttcagc catcatttct gttaaacttg attttccggc accaattgta
     4501 cctgctaaaa cgatcacgct catttacctc cagctcactt catcactaaa tattgtgttt
     4561 aactccaacc ttttttaaat atacccccaa tatctagtag tgtcaattgt attttttcta
     4621 gtcaaagaag aaaaaaagtc aaaaaattct taagaaaaaa gtgttttttc tttagaaaac
     4681 gtattcttag ttttacttct gagatggttt tagtaaaata aaggaggaat gttttttgag
     4741 acccttagtg gacaaaaaaa ttcgtgaagc agcgcaactc gttttgggcg ctttattttt
     4801 tgcttaaaaa tcacttttac gtgagaagga gttagattat ggcaaactat ggccaaggag
     4861 aatcaagcgg aaagatcata ttgatgggcg agcacgcggt tgtttatgga gaaccagcga
     4921 ttgcctttcc tttctatgca acaaaagtca ccgcattcct tgaagagctg gatgcaatgg
     4981 acgatcaact ggtttcttcc tactattcag gaaatttagc cgaagctcct catgcattaa
     5041 aaaatatcaa aaaattattc attcacttaa aaaaacagca tgacatccaa aaaaacttgc
     5101 aactgaccat tgaaagcacg attcctgctg aacgtggaat gggatcaagc gctgcagtcg
     5161 ccacagcagt cactcgtgct ttttatgatt acttagcatt tcctttgtct cgtgaaatac
     5221 tattagaaaa tgtccagctt tcggaaaaaa tcgcccacgg taatcctagt ggaatcgatg
     5281 cagccgctac tagcagcttg cagccgattt attttacaaa agggcatcct tttgactact
     5341 tttctttgaa catcgatgct tttttgattg tcgctgatac aggaatcaaa ggacaaacaa
     5401 gagaagccgt caaagatgtc gctcacctct ttgaaaccca gcctcatgaa actggacaaa
     5461 tgatccaaaa attaggatac ttgacgaagc aagcaaaaca agcaatcatt gagaattcac
     5521 cagaaacgtt ggcacagaca atggatgaat cacagtcact tctggaaaag ctgacaatca
     5581 gcaatgattt tcttaatcta ttgatccaaa cagcaaaaga taccggggca ttgggtgcca
     5641 aattaactgg cggtggacgc ggtggctgta tgattgcatt agcacaaaca aaaacaaaag
     5701 cccaagaaat cagtcaagca cttgaagatg cgggtgctgc tgaaacttgg atacaaggat
     5761 taggagtaca tacctatgtt taaaggcaaa gcacgcgcat ataccaacat tgctctaatc
     5821 aaatattggg gtaagaaaaa tgaagaactc atccttccaa tgaacaacag cctttcgtta
     5881 acattggatg cattttatac agagacagaa gtcatctttt ctgatagcta tatggtagat
     5941 gaattttatc tagatggcac cttacaagac gaaaaagcaa caaaaaaagt cagtcagttt
     6001 cttgaccttt ttcgtaaaga agccggcctc tcactaaaag cttcagtaat cagtcagaat
     6061 ttcgttccta ccgcagctgg attagcctcc tctgccagcg ggctagctgc tttagcagga
     6121 gcttgcaata ctgctcttaa gcttggatta gacgatctct ctctttcaag atttgctcga
     6181 cgcgggtctg gttcagcttg ccgaagtatt ttcggtggtt tcgttgaatg ggaaaaaggc
     6241 catgacgact taagttctta cgctaagcca gtcccttccg attctttcga agacgattta
     6301 gcaatggttt tcgttttgat caacgaccag aaaaaagaag tgtccagcag aaatgggatg
     6361 cgtcgaacag tcgaaacatc cagtttttat caaggctggt tagattccgt tgaaggggat
     6421 ctatatcaat tgaaacaagc aatcaaaaca aaagatttcc aacttctcgg agaaacgatg
     6481 gaaagaaacg gactaaaaat gcatggaacg actttagctg cccagcctcc attcacttac
     6541 tggtctcctg acagcttaaa agcgatggat gcggtgcggc aattgagaaa aaaaggcatt
     6601 ccatgctact ttacgatgga tgctggccct aatgtcaaag ttttagtgga aaacagccat
     6661 ttatctgagg tacaagaaac atttaccaaa ttatttagca aagaacaagt aattactgcc
     6721 catgctggtc caggaattgc gattattgaa taaagggaga tttttatgat tgaagtatct
     6781 gcaccaggca aactctatat tgccggagaa tatgcagttg ttgaaacagg acatccagca
     6841 gttatcgctg cagtcgatca attcgtaaca gtcactgtag aatccgcacg aaaagtcgga
     6901 agtatccaat ctgcacaata tagtgggatg cctgtacgtt ggacaagacg caacggagaa
     6961 ttagttttag atattcggga aaatcctttt cattatatcc ttgctgctat tcgtttgact
     7021 gaaaagtatg cacaagaaaa aaacattctt ttatcctttt atgatctgaa agtaacaagt
     7081 gagttagaca gttcaaatgg ccggaaatat ggattgggtt ctagcggtgc cgtgaccgtt
     7141 gcaacggtca aagccttgaa tgttttttac gcgttgaatt tatctcaatt ggagattttc
     7201 aagattgcag cactagccaa tttagcagtt caagataatg gttcctgcgg cgacatcgct
     7261 gctagctgtt atggtggctg gatcgctttc tcaaccttcg accatccttg gctccaagaa
     7321 caagaaactc agcattctat cagtgagtta cttgccctgg attggccagg tctatccatt
     7381 gagccattga ttgctcctga agatttacgt ttattgattg gttggacggg tagccctgcc
     7441 tctacttctg atttggtcga tcaagttcac cgttcgagag aagacaaaat ggtggcttat
     7501 cagcttttct taaaaaacag tacagaatgt gtcaacgaaa tgatcaaagg gtttaaagaa
     7561 aataatgtaa cgttgatcca acagatgatt cgaaaaaacc gacaattact gcatgattta
     7621 tctgcaatca ctggggtcgt catcgaaacg cctgctttga acaaattgtg taatttagct
     7681 gaacagtatg aaggagccgc aaaatcttct ggtgcaggtg gtggcgattg cggaatcgta
     7741 attgttgacc agaaatctgg catccttcct ttaatgagtg catgggaaaa agcagaaatc
     7801 actccactgc cgttacatgt ctatagcgat caaagaaagg aaaaccgatg aatcgaaaag
     7861 acgaacatgt ttcactagct aaagcctttc atgataaaca aaaaaacgaa tttgacttcg
     7921 tacggatcat ccataaccct ttgccgcaaa tagcagtttc tgatgtcgat ctaagtacac
     7981 aggcagttgg gtttacacta agcagcccat tttatatcaa tgcgatgact ggcggaagcg
     8041 aaaaaacaaa aaaaatcaac caagatcttg caatcgtcgc aagagaagca gatttgatga
     8101 ttgccactgg ttcagtcagc gctgccttga aagacccttc tttagccgat acttatacga
     8161 ttatgcgaca agaatatcct cacgggaaaa tcattgccaa catcggtgca gggacttctg
     8221 tcgaaagagc ccaagaagct atccgattgt ttcacgcaga tgccttacag attcatttga
     8281 atgcacctca agagttggtt atgcctgagg gagaccgtga ttttaccaat tggaaagtac
     8341 ttattcaaga gactcaaaca gccattgacg tccccctcat cgtaaaagaa gtcggttttg
     8401 gcatgacaag agaaacgctc aatgatctag ctgctttagg agttcataca gttgatatca
     8461 gcggtcgaag cggaacaagc ttcacccaga tcgaaaacgc ccgtcgctcc aagcgggaac
     8521 tgagttattt agctgactgg gggcaatcga cagtctcttc attgcttgaa gcaaatgaag
     8581 cagacacttc tatggaaatc ctagcctccg gaggtatccg caatgcctat gacatattta
     8641 aagccctttg tctcggcgca aatgcagtgg gtacttcagg aaccgtactg acgcatctga
     8701 tgaatcacgg cgtagaagaa actattatac tgatgaagca atggcaagaa gagcttcgtc
     8761 tgctttatac tatggtcggc gcaacgaata ccgcggccct acaccaacaa tcactgatct
     8821 tttccggacc agtgaaggat tggtgcgaag caagagggat cgacttagtg aagtatggaa
     8881 gacgcacaga aaagagcgtg ggacaaaagt aaagaacact tttatcccac gctctaaaca
     8941 cgtataaacg gcggaagca
//
LOCUS       DACGLW010000098         8936 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.103, whole genome shotgun sequence.
ACCESSION   DACGLW010000098 DACGLW010000000
VERSION     DACGLW010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8936
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.103"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            53..871
                     /gene="purR"
                     /locus_tag="IXT53_002520"
     CDS             53..871
                     /gene="purR"
                     /locus_tag="IXT53_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HAQ4906299.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            1374..2747
                     /gene="glmU"
                     /locus_tag="IXT53_002521"
     CDS             1374..2747
                     /gene="glmU"
                     /locus_tag="IXT53_002521"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HAQ4906300.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            2841..3812
                     /locus_tag="IXT53_002522"
     CDS             2841..3812
                     /locus_tag="IXT53_002522"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HAQ4906301.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     regulatory      3818..3920
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3986..4555
                     /gene="thiT"
                     /locus_tag="IXT53_002523"
     CDS             3986..4555
                     /gene="thiT"
                     /locus_tag="IXT53_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HAQ4906302.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     gene            complement(4636..5409)
                     /locus_tag="IXT53_002524"
     CDS             complement(4636..5409)
                     /locus_tag="IXT53_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906303.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            5676..7376
                     /locus_tag="IXT53_002525"
     CDS             5676..7376
                     /locus_tag="IXT53_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HAQ4906304.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            7346..7564
                     /locus_tag="IXT53_002526"
     CDS             7346..7564
                     /locus_tag="IXT53_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906305.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            7584..8474
                     /locus_tag="IXT53_002527"
     CDS             7584..8474
                     /locus_tag="IXT53_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HAQ4906306.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            8492..8776
                     /locus_tag="IXT53_002528"
     CDS             8492..8776
                     /locus_tag="IXT53_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906307.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
BASE COUNT         3016 a         1530 c         1883 g         2507 t
ORIGIN      
        1 aaaaacttat ataatgtaaa acgataaaat ttcttaaaag gagttggaat aggtgaaagt
       61 acgtcgcagt gaacgtctag ttgacatgac acagtattta ttgaatcacc cgcatgaatt
      121 gatttcctta acaagctttg ccgaacgtta tgaatctgct aaatcttcta tcagtgaaga
      181 tttgggaatc atcaagaaaa catttaaaga acgagggtat ggaaccttgg aaacaatccc
      241 gggtgctgca ggtggtgtat tgttcatccc tgaaatttca tatgaagagg cgaaaaaata
      301 tattgaatct ctagcagaac gtctatcaga acaagaccgt ctgcttccag gaggatatgt
      361 ttatctctct gatttgttag gagaacctga actgctgcgt caagttggaa gaatcattgc
      421 ttctaaatac cttggtgaaa aaatcgatgc tgtaatgact gtagccacaa aaggcgtacc
      481 tattgcccaa gctgtgtcct attatctgaa tgcgccattc gttattgtac gccgggactc
      541 aaaaataaca gaaggatcaa cagtaagtgt caactatgtg tctggatctt ctgaaagaat
      601 tgaaaagatg gaactgtcta agagaagttt gaaacgtggt tcacgtgtcc ttgtagtgga
      661 tgatttcatg aaaggcggcg gaacagtcaa cggaatgaag agcttgattg aagaattcga
      721 atcagaactt gttggcatca ctgtatttgc cgaatcaaaa ttcaacggtc atcgcgccat
      781 cgatgattat acgtcactgc tttatgtcaa agatgtagat actcaaacga aaaatattac
      841 ggttgtgcca ggaaattatt tttcagaata agaggttgtg aaaaagctgc ttagtttcga
      901 gtagtaagaa aaattttgtg aaaatggctt tccacatttt ttcataaatt tggcttaata
      961 ccgagaaact ggcttttgaa caccgtttat ctagaggttg tgaaatcagc gtcctgtcct
     1021 gagtattagc tcaataaacg gaaaaattgc tcctcatatt ttgccgttta ttgagctaat
     1081 accgaaggtc agctgattga aaaacgttta atgaagaggt tgtaagccta ccgtttaact
     1141 ctaagtcaca agaaacgatt tcaaaaaaca gcttttcatg ttttatgagt agtgtgactt
     1201 gtgaccgagg agttggcagg agagcaccgg ttaccaaata gataggcccg ttatggtcta
     1261 tctatttttt tatccagaaa acgctgggat aaaagaaaat cacttgtttt gttgaaaaaa
     1321 tgggtatact gggataaact gaaatcagca aactatattt aggagtgacg cagttggacg
     1381 cacgttatgc aattattttg gcggcaggga aaggtacccg catgaagtca aaattatata
     1441 aagttttaca tcccgtttct ggtaaaccta tggtagaaca tatcattaat cgggtcagtg
     1501 aaacgaaacc agatgaagtg attacgatcg ttggacacgg agcagaacaa gttaaagcac
     1561 aactaggaga acgcagtaaa tatgcgttac aagcagaaca attgggaaca ggacatgctg
     1621 ttttgcaagc tgcttctttc ttggaaggaa aaaaaggaac aacgttagtt atcagtggcg
     1681 atacaccatt attgactact gaaacgctga acaatctttt tgaatatcac caaggaaaaa
     1741 atgcaagtgc aacgatcttg acggcacaag cagaaaaccc aacaggatat ggacgaatca
     1801 tccgtgacca tattgggatc gtagaaaaaa tcgtggaaca aaaagatgca acaccagaag
     1861 aagctcttgt acaagaaatc aatacaggaa cttactgttt tgacaacgaa gctttgttcg
     1921 atgcgttaag caaagttgga acaaacaatg ctcaaggtga atactatcta acagatatca
     1981 tcgaaatctt aaaagaagaa ggccatacag ttgctgctta tcaaacggat gattttgaag
     2041 aatcaatggg agtcaatgat cggatcgcgt tagctaaagc aaatgagatc atgagaaagc
     2101 gaattaacca gatgcatatg gtcaatggcg taagctttgt cgattcagca acgacataca
     2161 tcgatgcagg cgtagaaatc ggtccagaca cattgatcga agcaggtgta caaatccaag
     2221 gaaatacagt cattggttct gattgtgtca tcgggtcaca ttcaaaaatc gtagacagta
     2281 ggattgagga tcacgtcgtc attgaaaact cagtcattga aagcagccat gtcaagaaac
     2341 atgcagatgt gggaccttat gcacatttac gaccaaaagc tgaaattggc gaaaacgtcc
     2401 atatcggaaa ctttgtggaa gtgaaaaatg ctcaaatcgg caaaggaact aaagtcggtc
     2461 acttgactta tgttggtgat gctacgctgg gagaagaaat caatgtcgga tgtggtgttg
     2521 tcttcgtcaa ctatgatgga aagaacaaac atcatacgac tgtcggcgac cacagcttta
     2581 ttggttctag cacgaatatc atcggacctg tcgaggtagc taaaaacagt tcgattgctg
     2641 ccgggtcaac aattacagac aatattccag aatatgcgct ggcaattgcg agagcaagac
     2701 aagtcaataa agaaggatat gcaaaaaaac tcccttatct aaactaagac ttttgcaaaa
     2761 tcacttgatt tatttaaaaa atatgactac tatttattag gtagaattct agagttgtga
     2821 aaaagaaagt ggaggtcccc atgtcaaaac attactttga cccaagatta aaaatttttg
     2881 cgttaaattc caatcgccca ttagcagaga aaattgcagc agcagtcggc gtcgagttag
     2941 gaaaatcatc tgttactcaa tttagtgatg gagaaattca agtaaacatt gaagaaagta
     3001 ttcgtggatc gcatgtctat gttattcaat caacgagcag cccagtcaat gataacttga
     3061 tggagttgct gatcatgatt gacgcactga aacgagcaag tgcaaaaaca atcaacgtag
     3121 tcatgcctta ttatggctat gcgcgtcaag accgtaaagc acgtgcacgg gaaccaatca
     3181 cagcgaaatt agtagcaaac atgattcaaa aagctggtgc aacaagaatg ctgactttgg
     3241 accttcatgc agttcaaatc caaggtttct ttgatattcc agtcgatcat ttgatgggtg
     3301 cgccacttat tgccaattat ttcttagaaa aaggaatcaa aggcgatgat gttgtcgttg
     3361 tttcaccaga ccatggtggt gtcacacggg cacgtaaatt agcggaattt ttgaaatctc
     3421 cgattgcgat catagacaaa cgccgtccaa aagcaaatgt tgcagaagta atgaatatca
     3481 ttggacaagt caaaggcaaa acttgtgtac tgatcgatga tatgatcgat acagcaggta
     3541 cgatcacatt ggctgccaat gctttgaaag aagctggtgc aactagtgtg tatgcttcat
     3601 gtacacatcc agtattatcc ggccctgctt tacaaagaat agaagattca gctatcgagc
     3661 gtctagttgt tacagactcg atctatcttc cagaagatcg taaaatcgac aaaatcgatg
     3721 aagtaagtgt tggcggacta atgggagatg caatcaaacg tatccatgaa aacaaaccag
     3781 ttagtccatt gtttgaaaca aaaaataaat aataaataaa ctcacaaagg ggagtccaat
     3841 tgggctgaga ttgaatctat ttctaaaccc ttcgtacctg tatcggttat gcgagcgtag
     3901 gaattgtgaa taaacagctg tttgactatt ttcgaaagct gcttcctcct ttgtgacttt
     3961 tttattcaaa ggaggaagtt tttttatggg aaaaaataga gtttgggtag aaggaacgat
     4021 tgttgcagcg ttagcgatgg tgttgtcgtt gattccgatc cagatcggca gcagtttttc
     4081 tatttcatta gggcagattc cattgactct ttttgcgtta agaagaggga caaaggcagg
     4141 gatgctggca ggtttcattt ggggcatcct acattttccg ttgggacaag tttattattt
     4201 aagtgtcgtt caagtgttga ttgagtaccc gattgcctat acttttgcag gcgcagcagg
     4261 gctcatggct tcaaatgtcc aaaaaggcta tgtagacgat aatacgaaac aaattagaaa
     4321 aagcatcgtg tttggtgcat tgattggcgc actttccaga tatttttggc attttattgc
     4381 aggagtggtt ttctggggag cgtatgcact atgggggatg aatccttggc tcttttcttt
     4441 cgtaatgaat ggtgccagcg ggttgagtac agctattgta acgatcatcg ttttattagg
     4501 gatccaacaa acgactcctt cgatttttgt gccagataaa cgacaggagc tgtaaataaa
     4561 aaagaagcaa gaaacttgag acagaatcat gtcctagttt cttgtttcct ttttattttt
     4621 tgataagaaa tagatctact tcaatggcag ataagaaaga tcggcatctt ctaccaattg
     4681 ccagtgtcga tcggtgacag agtagattaa agtagcgatg atttcctctt ctcgataccg
     4741 ggtgcctttt aatttccaaa tataatcttc aatcacatag attgggatac gatatttacc
     4801 atcgatcaaa ccaacttgat caaaagaaaa aatgatccct tcctgcatca aattacgcat
     4861 ttctttcaat aattccacac tgattttttc aacaggtgtt tcttttttga aaaaagattt
     4921 ttttacattg atcttatctg acaagatcga acttaattta ctagcaaaaa tcctataaaa
     4981 ttcatctaaa tagcgataat aaatccgatt cagctgatca ttaagatcga agtatacaaa
     5041 attattctgt agtttataaa aaaacggcga atgaagatgt gttttcatat gaccaaaata
     5101 taataattct gaaatttcta aaggtgtcaa ttctttcagc atcgttaaat cagtaaagtc
     5161 gatccacttc aattcatcga atccacgcct gttacgagaa gaggaaaaga aacgctgaat
     5221 attttctgta ccacggatta ctttcagccc tgtatgggat tcatattctc cagcagaagc
     5281 agatggatca agcaagagca agttttgagg aaaatgcacg atactgtttg caaagtcagt
     5341 agcagtcagc ccttttgtta aaacagcatt acttgtggta tcaatatgta cataaactga
     5401 ctcagtcatc atgttcctcc tcttatttcc tcttaatctc ttttatttta caacatttgc
     5461 attaaaaaag cttgtttctt ttattttaaa attccattac aaaatcacgt tttcattgaa
     5521 aacactctca ttttcaatta atttttgtga tttttgtatt tttttattta aaggaggttt
     5581 tatgaattgt tttcttatgg aatctacaaa gtcgaatacg agactcatga cctttttatg
     5641 acgtactggt atacttgaag aaaaaggaga gcgcgatgga gataaaaaaa gtttactttt
     5701 cacagaaaat gtcgctgtat tcgcctatcc agcaagcaaa acaacccctt ccattttttc
     5761 aaagtcatca agaaagtaga gacgcttttt ggcgttctgc aaaaaagcaa tgtgctttta
     5821 aagcagtatc gaaaaaatat accgtctatt ttactattgc taaaagtaga ggagatgtta
     5881 tttttaataa tctgttgatt gaaggtgaaa cttttgaatg gaaaaaattt tttcggtata
     5941 tggaagcttg cgctcgtttt tttattaaag aaaactgttg ttttttattt caatctcctc
     6001 tatcagaaga atggttgcgt atttttcata aaaacggcta tcaaggaacc acacaattaa
     6061 ataaaaagct ggtctatcat acagcacttg ttttaggtgg aggaggagcg cacggtgctt
     6121 atcagattgg tgtgtggcag gcgctgaagg aacatgatat caactttgag attatcacag
     6181 gtacctcggt aggtgcgttg aacggcgcat tgattttaca aggagatatg aaaaaagctg
     6241 tgaatttatg gaaaaagctg tcgacaagac aagtgcttgc gttaccagaa atggctgcag
     6301 ttgaagacct tcgtgaacgc ttctatcgag aaagacgcca aatgacgaaa acagcactaa
     6361 ttgaaggcgg ggtttcttct gctccattgg aaaagatggt caaagacaat ttggatttgt
     6421 ccttactcaa acataatccc aaaatacgtt tatatacggt gtctactaaa ctgccagaac
     6481 tagcggaagt agtgactgat atccaacaga caaaaacaga agaaatacca gattggatac
     6541 tcgcctctgc ttcattttat cccgctatgg cttatcgaaa gatcggaaaa aataaatatg
     6601 cagacggtgg gtatcgcaac aaaataccta ttgatattgc gattaacaaa ggagcaacag
     6661 aggcatttgt cgtagatgtg caagggccag gcccagcgaa aaaaattcgt atgccagaca
     6721 tttttatcca ttggaaatgt caaacattgt ggacactagg cagctttctg ctttttgata
     6781 gccaaaggaa ccaattaaat ttacaattag gctatctgga aatgaaaaaa cgattaggct
     6841 attactatgg gaactggtat acatttcatt ctgttaaacc tgcaagaaca tactggcgag
     6901 gatttttgtc ttacttaaca aaagaaattc agttagaatt atcttttttc aaaagcataa
     6961 aattttggca aaaactgcgt aatttatata agaacagggt cgtaccagag acatgcggat
     7021 tggcgatgct agaactttta gcaaaaaaac attttttact gcccaatgaa atctatcaag
     7081 tagacatgat gattcaaatg atctgccaac aagatataaa aagtatgccg gatgatttat
     7141 tggcacaaat tggtcaatta agtactgaag aatggcgaag gtatcgaaaa taccagcaaa
     7201 aaatacaaaa tgagcgcacg aaaactgttt attttgatta tctgctacaa ggaaaacgca
     7261 aggatcgttt gcagcatgaa ttgcttaagc aaccagttga cacattactg atcctatatt
     7321 tatattatct caaggaggaa caaccatggc acaagaattt tcatatgaaa tcgtagaaga
     7381 aatcgctata ttgtcagaaa acaacaaggg gtggcgtaaa gaactgaacc tagtcagctg
     7441 gaatggtcgc ccgccaaaat tcgatcttcg agattgggcg ccagaccatg aaaaaatggg
     7501 gaaaggcctg acattgacga acgaagagtt tgaacaactg caaaaagcaa tagaaaatat
     7561 gtaatgagga aggaccctgt cgaatgaaaa gtatgactgg ttttggcaaa gccatccgcg
     7621 aaacaaaagc gtatcagtta gatattgaaa taaaaagtgt caaccagcgt tttctagatg
     7681 ttcaaatacg aagtcctaaa atcctgaact ttgttgaaaa cgattttcgt cagcttatta
     7741 aacagcagtt aagccgcggt cgaatcgaag tatttgtgaa tctagcttat attggcaaaa
     7801 acgaaaaaca gatcgtcatc aactgggaat tgattgattc acttatcact caattgacag
     7861 acggaattca acaacgatac ggtagtcaaa atcaagtgtc aattagtcgg atggcggaac
     7921 gcttcgcgct ggacgaaaac ttcgtaacgg tagaagaaaa accggaagag agcatggaag
     7981 aattggcaca gttagctcta gatacattga aaagcgcact tgattctatt gaggcgagtc
     8041 gagaaaaaga aggtcaagct ttagctttag tgttgaaaaa gaatactgaa gaattcaaag
     8101 aagtactaaa aaaactcgat agttttgtag aaatctatga aaaggaattt caaataaggt
     8161 atcaaaaaaa gctggaggaa tttcttggag cgaaagtcga tgagcaacgg ttgttgacgg
     8221 agctagccat cttgttggaa cgcggagata ttcacgagga actggatcgt ctggcgatcc
     8281 atgtacaaaa actagatgaa cttatcgaag cacaatgtcc tgtaggtcgg gaattagatt
     8341 tcctgatcca agaaatgaat cgggaaatca acacgatcgg ttccaaatca aatgcaatcg
     8401 aaattaaaaa tcaagtcgtc cagtctaaga cgatcttaga aaaaatcaga gaacaagtac
     8461 aaaatattga atgacgaaag aggtagagat aatgaaatat tatgacgtaa cgttccacga
     8521 attaagtgga aaaactgtgg ttaaacggga tatcccttct gaaaaaaacg gctttgacgt
     8581 atggaaagat gcctgtgcag actataatga gaacgaactg tttattttga tcaacgacgg
     8641 tgcttacgtg acgatgaatc ggaaatttat tgttcgaatt gacacagaag aggtagaaga
     8701 tccgacagaa aaagcacgtt ctcgaaaaga tgagatcatg ggtgtagtga atactttgtc
     8761 caatatgggc ttttagcaag gatgctgaac aaagaaatac aataaataat cagaattgga
     8821 tttcttcgct tagcttcttt gctctaggaa tacatttaaa ggttgtgaca aaattcgtgt
     8881 cacaatcttt ttttattttg aataagtgat gttcaaaaac cattttctcg gtatta
//
LOCUS       DACGLW010000099         8731 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.104, whole genome shotgun sequence.
ACCESSION   DACGLW010000099 DACGLW010000000
VERSION     DACGLW010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.104"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            2..74
                     /locus_tag="IXT53_002529"
     tRNA            2..74
                     /locus_tag="IXT53_002529"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35..37,aa:Asp,seq:gtc)
     gene            78..150
                     /locus_tag="IXT53_002530"
     tRNA            78..150
                     /locus_tag="IXT53_002530"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:111..113,aa:Phe,seq:gaa)
     gene            155..225
                     /locus_tag="IXT53_002531"
     tRNA            155..225
                     /locus_tag="IXT53_002531"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:187..189,aa:Gly,seq:tcc)
     gene            238..311
                     /locus_tag="IXT53_002532"
     tRNA            238..311
                     /locus_tag="IXT53_002532"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:272..274,aa:Ile,seq:gat)
     gene            323..394
                     /locus_tag="IXT53_002533"
     tRNA            323..394
                     /locus_tag="IXT53_002533"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:356..358,aa:Glu,seq:ttc)
     gene            660..1853
                     /locus_tag="IXT53_002534"
     CDS             660..1853
                     /locus_tag="IXT53_002534"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HAQ4906308.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1983..3455
                     /locus_tag="IXT53_002535"
     CDS             1983..3455
                     /locus_tag="IXT53_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ4906309.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3538..4326
                     /locus_tag="IXT53_002536"
     CDS             3538..4326
                     /locus_tag="IXT53_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HAQ4906310.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(4393..5061)
                     /locus_tag="IXT53_002537"
     CDS             complement(4393..5061)
                     /locus_tag="IXT53_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HAQ4906311.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            5176..6888
                     /locus_tag="IXT53_002538"
     CDS             5176..6888
                     /locus_tag="IXT53_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4906312.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6891..8663
                     /locus_tag="IXT53_002539"
     CDS             6891..8663
                     /locus_tag="IXT53_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4906313.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         2622 a         1487 c         1944 g         2678 t
ORIGIN      
        1 tggttccgtg gtgtaggggt taacatgcct gcctgtcacg caggagatcg cgggttcgat
       61 tcccgtcgga accgtttggc tcggtagctc agttggtaga gcaatggatt gaagctccat
      121 gtgtcggcag ttcgattctg tcccgcgcca ttatgcgggt gtagtttagt ggtaaaacca
      181 cagccttcca agctgttgtc gcgagttcga ttctcgtcac ccgcttttag tttttatggg
      241 cctatagctc agctggttag agcgcacgcc tgataagcgt gaggtcgatg gttcgagtcc
      301 atttaggccc atttatttta ttggcccgtt ggtcaagcgg ttaagacacc gccctttcac
      361 ggcggtaaca cgggttcgaa tcccgtacgg gtcatggaag atactttttg agtatctttt
      421 tctttttatc tgaaagaatg attttaaaaa gcttagcaga gttatcttta tggatgattg
      481 tgctaagctt ttttggacag ttctaatgta aaatattttt atatttttta agcaaaaaaa
      541 tgaaaaaata cgagcagaaa actgttttat ctgctatact tggttcgttg caagttcccg
      601 aaaggatccg caggacggag atgccttgta accgaattta actaggggga atcattataa
      661 tggtagaaag acacttattt acatcagaat ctgtttctga aggacatcca gataaaattg
      721 ctgaccaaat cagtgatgca atcttggatg caattttaaa acaagatcca acagcacgag
      781 tagcgtgtga aacgtctgta acaactggtc ttgttttagt atttggagaa atatctacga
      841 cggcatatgt agatattcaa aaagtcgtac gggaaacaat aaaagaaatt ggttatacac
      901 gtgcaaaatt cggatttgat ggagatacag cagctgtatt ggttgcgatt gatgaacaat
      961 ctcctgatat tgctcaagga gttgatgaag cacttgagat ccgggatgaa gataaaaaag
     1021 acgtattaga tgagattggt gctggtgacc aaggtttaat gtttgggttt gctgtagatg
     1081 aaacaccaga attgatgcct ttaccaatcg ctttgagtca tcgtctggta cgacgcttgg
     1141 cagatttgcg taaatcaaat gaattaacgt atttacgtcc agatgcaaaa tctcaagtaa
     1201 cggttgaata tgatgatcaa ggacaaccgg aacgcgtaga tacaatcgtt atttcaacac
     1261 agcatgatga tgcagtagat aatgaaacaa ttcgtcatga tgtcatcgaa aaagtagtga
     1321 aggaagttat tccagctgaa ttattagatg atcaaacgaa atattatatc aatccgactg
     1381 gccgatttgt cattggtggt cctcaagggg atgccggatt aacaggaaga aaaatcattg
     1441 ttgatacgta tggcggttat gctcgtcatg gtggcggtgc tttttctggt aaagatgcga
     1501 caaaagttga ccgttctgcc agctatgctg cacgttatat tgctaaaaac attgttgcag
     1561 cagggcttgc tcgcaaagca gaagtacagc tagcttatgc gattggtgtt gctcaacctg
     1621 tttcgatctc aatcaatact ttcggtactg gaacggttcc ggaagaagaa ctgattgctg
     1681 cagtgagaga aaacttcgat cttcggccag caggaatcat cgagatgctt gatttgcgtc
     1741 gtccgattta caaacaaaca gctgcttacg gtcattttgg tcgtacagat gtagatttgc
     1801 cttgggaaca tacggacaaa gtagatgcgt tgaaagcaag tttagcaaaa taagttatat
     1861 tcagctggaa cgatcgccaa gtggtggttg ttccagcttt ttttatggca gttaacctct
     1921 tgaaaaagat agtaattatt tatctatttt tagatcctgt cataatgaaa gggaggaata
     1981 agatgaaaaa agaaacaaat gttactttgg tcacagtgag tattttcatt gcaacgttca
     2041 tgacggctat tgaaggtaca atcgtaacaa ccgctatgcc aacgattgtc ggttcattac
     2101 atggaatgga aatcatgaac tgggtatttt cgatttattt gctgacaaac gctatgctta
     2161 ctccgattta cggaaaactt gcggataaag tgggacggaa accaatcttt atttttggga
     2221 cattattgtt tattatcggt tcgttatttt gcggtttatc tcaaagcatg attgggttga
     2281 ttgctgctcg tgcgattcaa gggatgggtg ctggggcaat gatgccagta gctttgacga
     2341 tcatcggcga tctttattct attgaaaagc gagcaaaagt tttaggtttg aatagttcag
     2401 cctgggggat tgccagtgtt tttggaccgc ttgcgggagg attcatcgtt gaaacgatca
     2461 gctggcactg gatctttttt atcaatgtgc cgatcggaat tctattaatt attttgattt
     2521 ggctatattt aaatgaacca aaaatcatac atgagtctaa accaatggat atttttggta
     2581 gttttagttt gatggccatg cttgtctcat tgctgttagg gttccaatta ttaggagacg
     2641 aaggaatgag tccgcttgtt tctgctttgc tgctaggtag tatcgtaatg ttaggtattt
     2701 ttgtttttgc cgaaaaaaga gctaaagatc ctgtcatcat gatgcattta ttcaaaaatc
     2761 cactatttgt tgtggtcaac ttggctgcag cattggctag tggttttttg atgggaatcg
     2821 atgtttatat ccctatgtgg atgcaaggtg tattagggct gaatgcagca attggtgggc
     2881 tggtattagc accattatca ctagtctgga tggtcggttc tttccttgca agcaagtgga
     2941 tgactcaaat gaaaactcat tgggtcttga gaattggact actaatcgca gctattgggg
     3001 gcgcttggtt gtatcttatg cctttcagaa gtaactattt atggtttttt gctatatcaa
     3061 cggtcatcgg tataggctta ggtgtgacca tgactacctc gactgtcagt gcacaaaata
     3121 gtgtatctcg tgaccagctt ggtgtagcaa cttcttttaa tacgttagtt cgaacaattg
     3181 gtcaaacagt catggtttct atttttggtt tattgataaa tggggtaact aaacgagaac
     3241 ttgcagcagc aaatttaaca aatgatgcag atatcatgaa taaactggtg aatccacaaa
     3301 cggctaaata tattgatgcg gatattttgt taccattaag aaaaattctt tatgatgggt
     3361 tacattccgt ttatctagtt ggcttgttac tcgtgatcat tgctttggtc ttaacgcttt
     3421 tcgttcatga aaaaagtggt aaaataaaac agtagtttcg gttaagatat gtacgtagcc
     3481 gaatcattca aatttaaagt tattcaaaaa taacttctta ctaaggaagg cgaaaatatg
     3541 gcatttattc aagcaaatat ttattcgaat gtgttagaaa tggaagtcaa tctaaatgtg
     3601 atactaccgc aaacaacaaa aaaggtgatt ggcaccagtt cagagcaaga gacaacggat
     3661 atacctgttt tatatttact tcatggaatg ggtggcaatc atagtgtatg ggaaagacgt
     3721 acgtcgattg aacgttatgt agcaaactac ggattagctg tcatcatgcc atctactgat
     3781 cttggctggt acacagatac agtttatgac atgaagtatt ggacattcat tgcagaagag
     3841 ctacctgaga tttgtcatga attattcccg caattaacaa gaaaaagaga aaaaacatat
     3901 gccgctggat tatcgatggg cggatatggc gcgttaaaac taggccttgc aaaatcagaa
     3961 aatttcgcag cagtaatctc tctttcaggc gctgtttctg taggaaatcg aatggatgat
     4021 cttttgatgg ttcgtaaagc atctttttgg gaagggatct ttggaccatt ggatcaaatc
     4081 gaaggttcaa aaaatgatcc gcaatactta ttgaaagaac tagtaaaaag cggaaaacca
     4141 gttccaagat tttatatggc ttgtggggaa tctgattttc tttatcaggc aaatcaggaa
     4201 atggtatctg ctatgcagga acaaggcttg gcggtcacct ttgaacaagg acctggcgat
     4261 cataactgga tcttctggga ccaatggatt caacgtgcat tggcgtggct aatggaagaa
     4321 aaataaaaaa agagtcttaa aaagatagct cctatagaga tcacaaacat agtgatctct
     4381 ataggagctt ttttactgct cgttttctct tcttttattt tgttggcata agccttcaat
     4441 ataggtttgt gcactcgtta gaaagagtgc tttttcattc aattctgcat gggactgcat
     4501 actttcttta aatccctcta attgattttc aactacataa ttacgcataa atagaactgt
     4561 ttctttcaaa aattcatttc cgtccataat tttttgtcga agctgctgtt catgctgctg
     4621 atccattaac tgtccaatca ataaattgtg gagagaggca atggcctgat gactttgggt
     4681 gactgaaaat ccttcacgaa caagtaattg gatcatttgc tccaaatgtt ctaatcgact
     4741 cggaaaagcg ggcacagtat tgatttctaa ttctgcccca caagggaaac gtgtataaag
     4801 gtcatagtaa ttttccatat actgcagcaa ctgttcattc caaggcaaag ttggatctgg
     4861 aagcgctaat tcttcttcca ttgtttcagc cattttttgc aagagctgtt gtttgttttt
     4921 gaaataccaa tataaagcag gtgcttgaac atgaattctg tttgcgatgt ttcgcatgga
     4981 aagctgcgaa agagcttgtg tttcagccag gatatcaaag gcggcctgaa tgatgattgc
     5041 ttgagaaagc tttatttcca ttttatcaac ctcgtgtatt gcattatctt tttaaacatg
     5101 gtaaattgtt agtcggtcta attttacagt gttaaattag tcctgtcaat ttaaacaaag
     5161 gggtgtaatc aacgaatgaa attaatctta cgacatgcga aaaaatataa agtagctgtt
     5221 tttatttcct tgctgtccgt agcagtcatg gttgcagcag cattatggca acctaagtta
     5281 ctacagcaag tattggaagc tattattaca gaagataatg atgaaatgaa aacaattggt
     5341 atttacctga tcagtctggc tttgctagga ttagccgcag gtgtaacgaa cacgattttc
     5401 tctgcaaaag tagctcaagg cgtaagtgca gatatccgag aagaagcatt tcgaaagatc
     5461 cagacatttt catttggaaa tatcgaacag ttctctgctg gaaatcttgt tgttcgtcta
     5521 acaaatgata tcacgcaaat ccaaaattta gtgatgattt ctttgcaatc actttttcga
     5581 attccattct tatttattgg tgccttcatt ttagctatga tcacgatgcc gcaattatgg
     5641 tggatcatcg tagcgcttat cgttacggta tttctgatta cggcactttc atttacaagg
     5701 atgggcaagc acttcatgat cattcaaaaa ctgattgatc gtgtgaatag cattgcaaaa
     5761 gaaaacttac tagggattcg tgtagtgaaa tcttttgtac aagaagaaaa ccaattgaag
     5821 tcattcagca aagtaagtga ggacttaaca aaacacaaca tcatagtcgg tacattattt
     5881 tctgtgatga tcccgtcctt catgctggct gccaatttgg ctgtggttgg ggcgatcttt
     5941 tttgtgagcg atcttgttaa agatgatcct acgttaattg gtggaattgc ttcattcatg
     6001 aactacttga tgcagattat gatggcaatc attattggcg gaatgatgat gatgatgact
     6061 tcaagagcag ccgtatcgct gaagcggatt gcggaaattt tagatgctga accagatctt
     6121 acttatttag acgttccaga acaagagttg acgggttctg tcacttttga ccatgtttct
     6181 tttcgttatc ctggtgagga tacagatacg cttaaagata tcagcttttc tatcaaacct
     6241 ggagaaatgg ttggtatcgt cggggcgact ggtgccggga agtcaacact agcacaattg
     6301 atccctcgtc tatttgatcc gacagaaggc aaagttgaag taggtggtgt agatcttcgt
     6361 caagtaaatg aaaagagttt aagacaaacg gtttcctttg ttttgcaaaa agcaatttta
     6421 ttctcaggaa cgattgctca aaacttgcgc caagggaaaa agaatgcaac agaaaaagag
     6481 atggaacatg cttcttctat tgctcaagca aaagaattca tcgaaaagtt atctgatcgt
     6541 tacgaagcac caatagaaga acgtagcagt aacttttctg gtggtcaaaa gcaacgttta
     6601 tccattactc gtggcgttat cggtaatcca aagatcttga tcctagatga cagtacgagt
     6661 gcactggatg ctcgctctga aaagctggtc cgagaagcac tcgatcgaga tttgaaggga
     6721 acaacgacga ttgtcatcgc acaaaaaatt gcttctgttg tcaaagcaga ccgcatctta
     6781 gtattggatg aaggcagatt agtcggcgaa ggaacccatg aggaattggt agctggaaac
     6841 cgtgtctatc aggaaatctt tgaaactcaa aaaggaacgg aggagtaagt atgactgatc
     6901 taataaaagc aagtaaattt ttctatcact atttgaagcg gtacaaactc tcctttttct
     6961 ttatctttat cacagtgatc attgctacct atctacaagt aaaggcacct caatacatcg
     7021 gggaagcttt tcaagaatta gctaattacg taggaggctt gatgcaaggc gtagacgata
     7081 aaagcaaatt tgtttctgta atatggaaac tcttgctgtt ttatgtacta gctagtgcag
     7141 ccaactttat ctatagtatt ttgttcacac aagtagtagg gaagtcaacg aaccgaatgc
     7201 gtatcggtct attcaacaaa ttggaaaaat tgacgatccg tttctttgat tctcatcaag
     7261 acggtgaaat tttaagtcgt tttaccagtg acttagataa tattcaaaat agtttgaacc
     7321 aagctttgct tcaagtcatc acgaatgctg ttttacttgt cgggatcttg atcatgatgt
     7381 tccgccaaaa tgtagagtta gcttgggcta caattgcttc cactccagta gccgttttga
     7441 tagctgtctt gattatccgc aaagcacgga aatatgtgga tcttcagcaa gatgaagtgg
     7501 ggaaactaaa tggttatatg gatgaaaaaa tcagcggaca acgtgtcatc atcacaaatg
     7561 gcttacaaga agaaacgatt gaaggattct tagagcataa tcaaagtgtt cgcgaagcaa
     7621 ccttcaaagg acaagcttat tctggtctac tatttccgat gatgcaagga atgtcactgg
     7681 tcaatacagc gatagttatc tttttcggcg gctggttagc attaaatggt gatttggaac
     7741 gttcggtggc attaggctta gttgtgacat tcgtccagta ttcacaacaa tattaccaac
     7801 cactgatgca gatttcatct ggatacagca tgatccaatt agcgattaca ggggcaagac
     7861 gattgaatga aatatttgat gaaaaagatg aagtcaaacc agaaaacggt aaagcatttg
     7921 atggtgttga aaaaggtctt gcgttgcagc acgtagattt tggttattct ccagaaaaac
     7981 tagtcttgaa agatgtctcg atcaatgtgg acaaaggaga aatggttgct cttgttggtc
     8041 caacaggatc ggggaaaacg acgatcatga atctgctaaa ccgtttttac gatgtcaatg
     8101 atggttcagt agtcatcgat ggaatagata tccgagatta tgatcttgat gcattacgta
     8161 gacatgtagg aatcgttttg caagaatctg tattgttctc cggaacgatc aaagacaata
     8221 tcacattcgg caaaccggac gcttcagatg aagaagtcat caatgcagca aaacaagcga
     8281 atattcatga cttcatcatg acgttagaaa aaggttatga tacagagata tccgaagaaa
     8341 ataacttatt cagtacaggt caaaagcagt tgatcagtat tgctcgaacg atcatcacaa
     8401 atccatcttt attgattttg gatgaagcaa caagtaatgt agacacggta acagaagcaa
     8461 ggatccaaaa agcaatggat gaagcaatca aaggaagaac cagctttgtg attgctcacc
     8521 ggctgaaaac aatcttgaat gcagatagaa tcgttgtctt gcgtgatggg gaagtcattg
     8581 aagaaggcag tcatcatgag ttattagcag aaaatggatt ttatgcggaa ctttatcata
     8641 atcaatttgt atttgaaagt taaattaaaa gaacctcgtt ataacggggt tctttttata
     8701 tattttatgt tgtttgtttt tataaggggc a
//
LOCUS       DACGLW010000100         8504 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.105, whole genome shotgun sequence.
ACCESSION   DACGLW010000100 DACGLW010000000
VERSION     DACGLW010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.105"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            complement(138..1091)
                     /gene="menA"
                     /locus_tag="IXT53_002540"
     CDS             complement(138..1091)
                     /gene="menA"
                     /locus_tag="IXT53_002540"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HAQ4906314.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(1104..2207)
                     /locus_tag="IXT53_002541"
     CDS             complement(1104..2207)
                     /locus_tag="IXT53_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAQ4906315.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(2297..3232)
                     /locus_tag="IXT53_002542"
     CDS             complement(2297..3232)
                     /locus_tag="IXT53_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HAQ4906316.1"
                     /translation="MKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSESSTAQSST
                     AKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKYDNVNENG
                     ESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSSESFQNYA
                     QQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGKVTESNFD
                     YIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPANVEVVTGA
                     THSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            3534..5468
                     /locus_tag="IXT53_002543"
     CDS             3534..5468
                     /locus_tag="IXT53_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ4906317.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5925..6341
                     /locus_tag="IXT53_002544"
     CDS             5925..6341
                     /locus_tag="IXT53_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HAQ4906318.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            6355..6921
                     /locus_tag="IXT53_002545"
     CDS             6355..6921
                     /locus_tag="IXT53_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HAQ4906319.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            6944..7930
                     /locus_tag="IXT53_002546"
     CDS             6944..7930
                     /locus_tag="IXT53_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HAQ4906320.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(8085..>8504)
                     /gene="scm"
                     /locus_tag="IXT53_002547"
     CDS             complement(8085..>8504)
                     /gene="scm"
                     /locus_tag="IXT53_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HAQ4906321.1"
                     /translation="STDTSASSDSTDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSS
                     TTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKR
                     ALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
BASE COUNT         2509 a         1641 c         1722 g         2632 t
ORIGIN      
        1 acctcctgat aaacggtgtt caaaagcttg catctgcggt aataagccca accaaacgaa
       61 aaatataagg agctattttt atttggctgt tcttattact tgatgcaggg cagttttttc
      121 acaacctctt catgctgtta aatggcttgc ataatgattg ttagtaatag ccccaataca
      181 taactggcat tgaataagac catatttttg atggaatagc tgaaactgat aggctggggt
      241 aacgtttgct tgaattgatc caaatgcttc ttcacaattg gcaaggtcaa gaaggtgacc
      301 aagatcggcc attcatagat accgctcaac aaccccatta aaatcacgaa atagcaagca
      361 tacatcaata gctggaataa gactgttccc gcaggacgtc cgatataaaa aactagtgta
      421 tagcgatgat tttcaatatc acggtcaaga tcccgcaaat tattagccaa catgatattg
      481 gcaatcgtaa acaccaaggg taaggaagct aatacgattg ctgctatagc ccaaagattt
      541 cctaccaaag caaattgtcc agatgaaaag ttgactgtca gatcatagac atctgaaccg
      601 aatgtattga cataaacgac catggcaaaa attccaaggc ccatagtcac cccactgaaa
      661 atttccccta aaggcattcg agataaagga atcggtccga atgtatagaa tactccaata
      721 aaacaacaga taaatcccat caaaagtaaa agccaacccg tattgatcgt tagaataacg
      781 ccgataacaa aaacaaatgc aatcatagct aaaatcatgt ttcgaatcaa tacaggagat
      841 agatttctgc ggccaatgac attttcttca tacttatata cttcagaatg agccttttca
      901 taatccatgt aattattgat agcggtagtc gctaaatcaa aaactagcat tccaataaaa
      961 aataaaaaag tgtttagcca atgtatctca tgaaaaaaag cgatagagaa taatacccct
     1021 accgcaaaag gaaatacact agccaccttt gttcgtattt caactacttc taaaaagatt
     1081 tttagtgtca tggttcgtac tccttatttt agttcagaac ggtcacccat cgtgtaatgg
     1141 gagtcctccg ataatttgaa ggtgtctttg attggatcgg tcacatatac tttatcgtct
     1201 tttgtcacga agatcgcttc cgtcccgtta ttcaattcgc tttcgacgta ttccagtcct
     1261 tttttcacac ccattgaaaa gactgctgta gacaacccgt caccatcaat tgatttatct
     1321 gttatgatcg tgacacttgc gatatcattg tcgaacggat aacccgtttt tggatcaaac
     1381 aaatgatggt attttttgcc atctacttct aaataacgct cgtaaattcc agaggtaact
     1441 aatgttttgt tccgttcttt aatagaacct aaaacagatc cgcgagccat gttcggatct
     1501 tggatgccga ctgtccagtc ttggttttcg cctcttgggc tgtggcctaa gacatagaca
     1561 ttaccgccca agtcaacgat ggcagtggtc actccttgtt ttttcaagac tttcactact
     1621 tcgtccgtaa tgtaaccttt ggcgattgca cctaaatcga tgatcattcc tttttctttt
     1681 agataaacag ttttttcttt gtcatttagt tcgatcttat gataatccac taatttcaat
     1741 gcctgatcga tttcttcttg ggaaggtttt ctggcgtcat caaaaccgat tcgccataat
     1801 tgtgtgattg caccaatcgc catatcgaat ccgccttgag aatcttggct atattcgtag
     1861 gcccgcttga ctaacgtgta gacgtcatca gaaactttta ctggtttgat cccagcttgc
     1921 tcattcactt catcgatctc agaacctttt tgattgatcg tgattttatc accaagttct
     1981 tttactcgat cgaaggcagg tttcaatgct gattctttgc cttcatcata cacacggata
     2041 cgaacgtaag tccctaacaa gaactgttca tcagaatatg gatctttcaa tatcttggta
     2101 gctgctgaac tttcagtggt tgttttattt gctgagttgc tgcagccagt tagcaccata
     2161 aaagagatgc ccagtgtgat taaggaaaat agtagttttt tccgcatctt tttcctctct
     2221 tcattttgaa aggtgagacg actgcctaat ctggctatcg tcccaccctc caacttttat
     2281 tagtatgatt atgtgattat ttttctgtta cgatattatc tacttcgatt gtttgagtgt
     2341 cgcctttttc agctgcattc actaattgtt gtgcgtacat gatgaatgaa tgtgagctgt
     2401 gtgtagcacc tgtaacaact tctacattag caggtgagcc atcttcttcg cccattgctt
     2461 tgacgaattc gtcgttcaga gtagggatgt aagtttttgg ttctgtaccg ctttttgctt
     2521 tcatattttc attatattct gtatcatctt gttttgattt accgtctttg tcgatataat
     2581 caaagtttga ttcagttact ttgccgccag caactgtcat ttcgaattgc acacggtagc
     2641 cgttagagta gttcttttct tcaagcttgt aagtgccgtc tttcaaatca gcaccattgt
     2701 cgatttcgat tgtatctgtg ttaccagctt gagcagcttg gatcaattgt tgtgcgtagt
     2761 tttggaagct ttcagatgag tgagtagctc cagttacgac ttccacaccg cttgcgcttt
     2821 gtgcttttaa gaattggtca ttcaactcag gaatgtattt ttctgggttt gtgccagatt
     2881 ttgcttccat gtttttgttg tattctgcat cttttgtttt tgattcgccg ttttcattga
     2941 cgttatcata ttttgattca gtgattttgc cgtcttttac aaccatttca aagactgcac
     3001 ggtaaccatt tgaataattt ttttcttcta atttatatgt gccgtcttgc aaatctccgc
     3061 cagctacaac ttttgcagat gattctgttg tgctttttgc agtcgaagat tgtgctgtag
     3121 atgattcaga agaagagttt gagcttccgt tgtcatctgc gccgcaagcg cctaacacta
     3181 gagctgaaaa tgctaatacg gcaaatccag ttacaaaacg ttttttcttc attttaatac
     3241 cccaccattc catttctagt ccacaatttt tctttgtgat tcattataca ttctaatctt
     3301 tttagcctat aagtcaacta tttttgataa ataacccatg taatatttca aacgttttct
     3361 ttcagcaaat ttcgtgaaaa aacgctttat tttaaaattt gaaaaaaatc caaaaggagt
     3421 tagacaaaag aacagaacat ctatataata tatggagatt gtgtaataag tatcaaacta
     3481 aaagaattat catatattca ttatatagat agtctataag gagcggatga tcaatggcaa
     3541 agcaaaatat tgtcgttgta ggagcaggat atgccggtgt ttcagctaca aaatacttgg
     3601 caaagaaact gaaaaaagaa gatgtagcaa ttactctgat cgatcgtcat tcgtaccata
     3661 ctatgatgac cgagttgcat gaagtagctg gcggacgtgt agaaccagaa gcaatccaat
     3721 acgatctgca gcatttgttt gctcgtcaaa agaatgtaca acttgtcaca gacactgtaa
     3781 tcggtatcga taaagaacga aaaatcgtca agaccaaatt aggttcctat ccttttgatc
     3841 agctgatcct tggaatgggc ggagagccga atgactttgg tacacctggt gtcaaagcac
     3901 acggattcac cctttggtca tttgaagatt ctgtaaggat tcgggaacat attgagaaga
     3961 cagtagcaaa agctgcattg gaaccagatg atgaactgcg taaagccatg ctgactttcg
     4021 ttgtctgtgg ttctggattt accggtattg aaatgattgg ggaactgatc gactggaaaa
     4081 atcgcctagc caaagattat aaattagatc caaacgaaat cacactgatg gtcgtagagg
     4141 caatgccgac gatcttgaat atgttggatc gaaatgatgc tgcaaaagct gaacgctatt
     4201 tgcgtaagca caacgtggaa ttattattga atgcaccaat cgtagaagtg gctgctgatc
     4261 atatcaaatt gaaagacgga cgcaacattc cgactcacac actgatttgg acagctggtg
     4321 tcaaaggaac ttctgacgct gctgactttg gattagaatc agcacgcgga cagcggttga
     4381 tcgccaatga atatatgcag gcaaaaggct acgaagataa aaacatctat atcattggtg
     4441 atttagtctt ttatgaagaa ttcccaggaa caccgacacc gcagatcgtt caagctgctg
     4501 aacaaacagg acacacagcc gcagcgaata tcgtggcttc gatcaaaggc ggagaaaagc
     4561 ataaatttaa aggaaactat caaggattta tggtttctat tggtgccaaa tggggggtag
     4621 ctaacctatt tgataagatc catttaagcg gatttttagc tatcgtaatg aaacatatcg
     4681 tgaatttgaa atacttcttt gatattcgtt ctggttatta tatgttccaa tatttaatgc
     4741 atgagttttt ccatatcaaa gatgatcgca acgtgactcg aggacattct tctcgttacg
     4801 gcaatgtctt atggagtgtg ccacttcgta ttttctatgg tttagtttgg ttcgttgaag
     4861 cttccaaaaa aatcattggg aatggtgaag tgttcaaacc aagtacttgg tttggtgaag
     4921 gatcatggtt cactggaaac agtgtgttcc cttggacatg ggaatacaat gtaacagatg
     4981 ttgccagtgg tgcttctgct gctggtgatg caaccacagc tgccagcgga gcaggggcaa
     5041 gtgccggaga agcagcgact caagctgcac attttggttt gagttatgca tacggtgaag
     5101 aaccaatggc tgtgattgag aagtcaccag actggttcac aaagatcatg caagtgatca
     5161 tgccaaacaa tgatgtagca atgttcttcc aaaaattcat ggtattcttt gaaattgcct
     5221 tggcattagc attgattgct ggattattca catggctatg cagtgcgaca acgattgtat
     5281 tggtgatcac gttctgctta tcaggcatgt tttactgggt aaacatttgg ttcatttttg
     5341 ttgctttcgc attgatgaac ggttccggac gtgcgattgg gttggatcgc tgggtaattc
     5401 catggctaca acgtacacta ggtaattggt ggtacggtag accaagttcc cagtacggca
     5461 agaaataaat agataaagaa cgttatttga atgcaagatt tcaatagaaa ggggctgaga
     5521 caaaccgtca cagcctcctt tttatgtaaa attaaaggaa taagatgccc gagataagcc
     5581 tccatgagca agacacaggt gtcacaacct ctgttacaac ctcttgttat aaacggtgtt
     5641 ccagaagtag cttcttcgaa aaataagccg aaatttcaca aaaacttgaa aagtaatttt
     5701 cgtaaattcc ttcttatttc ttgaagttgc ccacttctgt cacaacctct tgttataaac
     5761 ggtgttccac ctgctgcttc tcggtaataa gcccaaagaa ttagaaaaca tggaaagctg
     5821 ttttctattc ttcgttctta ttactcaaag caagacacag gtgtcacaac ctcttgtttt
     5881 tatgctaaaa ttgggtgagt taatctggaa aaaggagaac aaaaatgagc cttaaagaat
     5941 ttgctgaaaa aagtctgctc aaaccttggg atgtttttat tattttgctt ttgactgttc
     6001 tttctttttt accagtcatg atttttggtc ttcagcaaaa caccgcaaca gcagaaaaag
     6061 aagcggtact gcgtgtggat ggcacagaaa tcaaaacatt ccctttgatg gctggagaag
     6121 agagcaacac atatacttac aaagatgaac acggagatta taacttgatc gagatagatg
     6181 gcgatcgagt acggataaaa gaagcagatt gcgatgacca gatctgcgta cgtcgaggct
     6241 gggcaacgaa aaatggcgaa accatcgtct gtttgccaca taaattagtg atcgaagtcc
     6301 gttcaacaga tggaggtgac gaagacagtc tgatttatta gacctttaca caccatgagt
     6361 aaattacaaa aaatgattta tatttcgcta cttgttgcac aaggagtgat cattggttta
     6421 ttggaaaata tgatccctta tcctttttct tttgcgcctg gtgcaaaact aggcttagcc
     6481 aatctaatca cgatcgttgc catttttacc atgaaaaaac aagacagctt cttactactt
     6541 tggctacgac tattcctgac tactttgcta ggcggaacga tttccacatt cctttatagt
     6601 atgagtggtg ctttgcttag ttattttgga atgttattgg tcaagcaatt aggaccaaaa
     6661 cgtgtcagta tcatcggaat tagtgcggcc ggcggtttca tgcataatgt cggacagctt
     6721 ttgatggctt ctttcattgc acaatcatgg acagtcatgt tgtacttgcc agtcctttca
     6781 gttttaggga ttttatcagg gattgcaatc gggatcgcag cgaattatct tttacgacat
     6841 gtgcagacac ttcaacgatt ccaaaaagat tatgagcagg gaaccaaaca aaaatggagc
     6901 ccaatgttta ccaaaaaata atcgtcacac aaggagtttt tttatgaagc tacatccaat
     6961 gtgggaaaac tatcctgctt taagtaaaga attgacagaa acactaaaac ggatggaaga
     7021 aactgtccgc ctgaaaaaca aacctgtcga acaagcaatc aaagaaacga tccatgccgg
     7081 cggaaagctg ttgcgtcctg cctatcagct attgttctca caatttggac cagagcaaga
     7141 tcgagacaaa gccatcgctt tggcagcttc tatcgaaatg ctccatacgg ccacactgat
     7201 ccatgatgat atcgttgatg aagcggatct gcgacgtggt cagcctaata tccgtagtca
     7261 gtttggtaat gtcgtttctg tctatgcagg agactattta tttgtctgct gttttaaact
     7321 attgtctgat tactccactt ccttgaaaag tctccaattg aattctcgca gtatggaaaa
     7381 agtattgaat ggcgaattag ggcagatgga tgatcgctat aattatgaac tttctgtaaa
     7441 agaatatttg gaaaatattt caggtaagac agctgaactg ttccaattaa gttgttctgt
     7501 tggcgctttt gaaagtggaa caagcgagcg cttcgcaaaa aaagcaggag atatcggact
     7561 ttctatcggg atggcctttc agattatcga tgatatttta gattatacaa aagagagcca
     7621 ggaaatcggc aaacctgtac tagaagacat gcgtcaaggg gtctattctt taccgttgat
     7681 ctactctttg caaaaaaaca aaccaaagct actaccttat ttagagaaga aagcggcaat
     7741 gacagaagaa gatgtcgacg ccgtacgcaa aatcgttgaa catacaggag gcgtggaaga
     7801 agctaggaag ttagcggcct cttacacaga aaaggctttg aaggaaataa agaaattacc
     7861 tgccacctct cttcgaacaa aagagaatct cttttccctt acccaactga ttttggatcg
     7921 caaagactga atagggaaag gttcgatacg taaataaatt tctcagaagg aaatctacgt
     7981 gttttttgac aaattgaatt gtagttcttg tatttatgat aaaaagcaaa aataaagagc
     8041 aacgtccgtt agcgacgttg ctctttttta tcttgaatat ttttttacct tttcgatttc
     8101 ttataactca taaaagtgat tcttaaaccg attacaagta atataacgcc tgcgagtgtt
     8161 atccaattat tcgattgact tccagtttta ggaagtgctc tttttgattt gtttccattg
     8221 tcattgcttg ttttattagt taagttaaag ttcgtcctgt tatccgaact tgtttttgag
     8281 aaggagacac ttgcatgacg tgtatcgctt gtggtagacg acgtgtctgt actatcgctt
     8341 gaagttgatg aatctgagct atcacttgtg gtagaggatg tatctgtact gtcacttgaa
     8401 gctgacgtgt ccgtgctatc acttgtagtg gtagatgaat ctgagctatc acttgtggta
     8461 gaggtagtat ctgtactgtc acttgaagct gacgtgtccg tgct
//
LOCUS       DACGLW010000101         8297 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.106, whole genome shotgun sequence.
ACCESSION   DACGLW010000101 DACGLW010000000
VERSION     DACGLW010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8297
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.106"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            complement(52..1518)
                     /locus_tag="IXT53_002548"
     CDS             complement(52..1518)
                     /locus_tag="IXT53_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HAQ4906322.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
     gene            1905..2501
                     /locus_tag="IXT53_002549"
     CDS             1905..2501
                     /locus_tag="IXT53_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ4906323.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(2559..3041)
                     /locus_tag="IXT53_002550"
     CDS             complement(2559..3041)
                     /locus_tag="IXT53_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906324.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(3147..3344)
                     /locus_tag="IXT53_002551"
     CDS             complement(3147..3344)
                     /locus_tag="IXT53_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906325.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            3639..4397
                     /locus_tag="IXT53_002552"
     CDS             3639..4397
                     /locus_tag="IXT53_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HAQ4906326.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            4457..4885
                     /locus_tag="IXT53_002553"
     CDS             4457..4885
                     /locus_tag="IXT53_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906327.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            4931..5003
                     /locus_tag="IXT53_002554"
     tRNA            4931..5003
                     /locus_tag="IXT53_002554"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4964..4966,aa:Gln,seq:ctg)
     gene            complement(5153..5524)
                     /locus_tag="IXT53_002555"
     CDS             complement(5153..5524)
                     /locus_tag="IXT53_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HAQ4906328.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            5739..5924
                     /locus_tag="IXT53_002556"
     CDS             5739..5924
                     /locus_tag="IXT53_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906329.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            complement(6007..6195)
                     /locus_tag="IXT53_002557"
     CDS             complement(6007..6195)
                     /locus_tag="IXT53_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906330.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(6219..6482)
                     /locus_tag="IXT53_002558"
     CDS             complement(6219..6482)
                     /locus_tag="IXT53_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906331.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            6640..6897
                     /locus_tag="IXT53_002559"
     CDS             6640..6897
                     /locus_tag="IXT53_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HAQ4906332.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            complement(6968..7159)
                     /locus_tag="IXT53_002560"
     CDS             complement(6968..7159)
                     /locus_tag="IXT53_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906333.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(7300..7620)
                     /locus_tag="IXT53_002561"
     CDS             complement(7300..7620)
                     /locus_tag="IXT53_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906334.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(7643..8077)
                     /locus_tag="IXT53_002562"
     CDS             complement(7643..8077)
                     /locus_tag="IXT53_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HAQ4906335.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
BASE COUNT         2634 a         1636 c         1314 g         2713 t
ORIGIN      
        1 aaaaaacagg aactgctgca aataagcaat tcctgttttt atgttcattg attaatgaat
       61 agagcccatt agttttttga ttggttcttt cacagtaaat agcaagatac caccgacgat
      121 tgctactgca ccgataatac cgaagtaagc agattctgtt cccggtgtgt agaagcgtgc
      181 aatttgtgca ttgattgctt gtgaagaagc atcagctaag aaccaaatag cgattgtttg
      241 tgattcaaat gctcgtggtg ctaattttgt tgtcacagac aaaccaactg gtgaaagaca
      301 caattcagca ataaccacga tgaagaaact tcccaacaac cacaatggac ttacttttcc
      361 acttgtacca taaagcatac ccggcaacat caatagcaag tacgataatc ctgcaaataa
      421 aagtccaaga gagaatttca ctactgtaga tggttgacgt tcaccaagtt tcgtccacat
      481 cgttacgaat actggtgtca gaataaagat gaaaattgga ttcaatgatt gataccagct
      541 tgcttggatc gttacaccaa atagcgtaga ttgtgtacgt tcactagcaa ataaagctaa
      601 gatagatgaa ccttgttctt gtaatgacca gaaaatgatt gctgcaagga ataatgggat
      661 ataagcccat actcttggtt tttcttcagc agttacatct tttgaagtca gcattttgat
      721 aaagtaatac aacggtaaga taatccccaa tacactgatt gtattgacaa aaaagtcaat
      781 tgtcagatga ccagttagtt gtgccccgcc gaatactgca gcgataacaa caagtgcgat
      841 gatcaagtta cggatgaatc ctttacgttc ttcagttgtc aaaggattcg gcggtgttgt
      901 acccactccg tctaatgtct tgcgtccttg gatgatgtat tgtaataatc caaagaacat
      961 cccaatagca gctaaagaga accctaaatg atagttatat tcttgaccga ttgtaccaac
     1021 gattagtggt gctaataatg cccctaagtt gacacccatg taaaaaatcg aaaatcctgc
     1081 atctcttcgc aagtcagtct ttgaataaag atgtccgacc atgccagaaa catttggttt
     1141 caaaaaggcg gttccgataa taatcaatgc catcgaaaca aacaatgtac ttacaccaaa
     1201 tggtaaagcc aagacgatgt ggccaagcat aatgaaaact ccaccgaaga atacagtgcg
     1261 ttttgctcct aacactcggt cagataccca tccgccgacg atactagaca tgtagatcaa
     1321 agatccgtag atcgacatga tagctaatgc tgttgctcgt ggtaatccaa gtcccccgtt
     1381 ggcaactgta tcgtacatat aatagataag taatgcacgc ataccgtaat aactaaatcg
     1441 ttcccacatc tcagtgaaga acaaagttga caatcccttc ggctgcccga agaagccctt
     1501 gtcttgctgt ttttccataa tctatacccc aattctttaa attttcagaa aatattatac
     1561 atctttaccg aaaaatttaa ctaaaaaact tatttaagaa atataaacta attttctata
     1621 aaagcaagta atttttaatg gcaatatcat aaaaaaaaga agatagagat ttaataatta
     1681 tatatcagct gtttttttca aataagcaaa aattcctaaa acacaacaat gaaagcgata
     1741 actaagaaaa tccttacata acgatttttc ttattaaaat tgttttttga acttcgctta
     1801 ttttttgtga gcatatctgt ttacttactt tttgtaaggt gctatacttc atttcgtaat
     1861 caattaaaaa aatgcaaaac gcaaatcaaa ggagaaattt tattatgaca acttttactg
     1921 acactttgaa aaaccgccgt tcaatctatc atctaggacg taatgtttca ctttctaatg
     1981 aagaattaac aactttgatc aaagaagcaa ttaaagaaag cccaactgct ttcaatgctc
     2041 aatcaacacg cgcagttatc cttttcggag atgcacatga aaaactttgg gaaatcactg
     2101 aagaagcact tcgcccattg actccagcag aagctttccc aaatacacaa aacaaattag
     2161 ctggtttcaa aaatggttat ggtacagtat tattcttcaa agatactgac gtggtaaaag
     2221 gtctacaaga acaatttgaa ttatatgcag acaacttccc agattggtca gaacaatcaa
     2281 acggaatcgc aacagctaat acttgggtag cattagttga taaaggattg ggcgcaaact
     2341 tgcaacatta caacccagtt attgacgaag cagttgcaaa agaatggaat atcccttcaa
     2401 actggaaact acgttcgcaa ttagtattcg gttcaccaga aacaccggct ggggaaaaag
     2461 aatacatgaa cgatgccgat cgcttccgcg tatttggata attttatttg aaataaaaaa
     2521 cagaggttgt gacaaacatc ttgtcgcaac ctctgttttt attctggatc aatcataata
     2581 tcaatctctg cctcatcgtt taagcgttgg gacaaaaatg gaactaattc ttctaatgat
     2641 gcagaaaatt tcagcatcgg tcctcgccat ccgtttcgga aaaaattcat ttttacgatt
     2701 ttagaacctt ttggctgata taatacaatt ttattgatct cagaaaaagg aacacctttt
     2761 ttgtcaaagc tattcaaaac aagtccttct tctgtgattc ctctactgtc caacaaaaaa
     2821 ctaataaaaa tcattgctga caagatccct tttgccaatt catctgctgt aggatgactg
     2881 atgagtgtat accaaatgac tagtgacact aagaagggta tcaccaagta gacccatcga
     2941 tttcgcacag tccgataaat aatttctttt cgaaccagcc aaagaaataa aaacatcaca
     3001 acaacgaata aaaacaatag aaccgtaaag aatgttacca tgacaactcg cctcgctttt
     3061 tcttctccag aaaataggaa aagaatactg atttaccttc tcaaagaaat tggacttgca
     3121 gcacgccgca cgtccaattg ttttctttat tcaaatgtat catattttgc aacaaccatg
     3181 cttgcttttt catctaataa ttcttggtcc tctgcagcac attgggcaac tttgaccacg
     3241 gcgcagtttg tcatttttga tacaacttca ccaatgaccg tttttgtaaa accaattggt
     3301 ttgcatgcat attgttctcc taaattaatc atttcttccg tcatcaaaat cctcctaaac
     3361 tgctggaact tttctccaca ttaccactgc tatttctttg gagatttaac tcattgattt
     3421 ttatttacag ttaaaaatca accttacagg cattatagcg tactgttttc gttttcacaa
     3481 ttaaaacagc tgttgtattt agaagaattt ttttcaaaaa attaaaacat attccttaaa
     3541 aactaattat ttcctacata ttaaaatata atacttccat ttccaataaa aatacattta
     3601 taataggaaa gataaacagc tgaaaaggga gatttcaaat gaagaaaaaa acgttcaaaa
     3661 aactattgtt tctagggggc acagctgctc ttttattaag cggtacaagt tattacctac
     3721 atatccattc tccagaacat acaatgaaca ctgcacaaaa aagttcaaca caaaaaacga
     3781 aaaagacaaa agagaaagca gaagttttac tagatgttcc attagagagc cagtttgatt
     3841 ctccttcttt agaaaatggc tgtgaagtca cttcgctttc tatgctttta tctttttatg
     3901 gttatgaaac aactaaaaac cagttagctg agcaattgga ttacgtccct gttttcaatg
     3961 cagatgggac tcatggcgat cctaatgaag gatttgttgg tgatatcagt ggcggagact
     4021 gggctatggt cgtctatgtt cccccagttg cctcccttgc tcagcaaatc gtccaaacgg
     4081 attatcacac ttttccaaaa accgatgctc aactcgatga tatcaaaaaa gcactatcta
     4141 aaggaaatcc tgtctggact tctgtgacta tcaattttga agtcccagat gaaactgatt
     4201 ttatgacttg gaccacaaat aatggagaag taagagtcac tcctcttgtt catgcttgtg
     4261 tagtcactgg atatgacaaa gaaaatattt atgtcaacga tccctatggt gtaaaaaatc
     4321 gagcagtacc gatcaacgat ttttcggcga ttttcactgc aatgggtgga cagatgctaa
     4381 ctttggagaa aagctagtaa ttcaaacaaa aatcccttat actcaagata ccaaaccttt
     4441 gaagggagcg tagataatgg aaaaattcac acataaaaaa atggatccaa atgaaattcc
     4501 gattattttt gtccgtgact gcaagggaaa tgttcaaggt aaagtctcta ttaatgagtg
     4561 gaacgagcgt cgcagacctg ctaccctcaa tgaactggaa atcaaattgt atcgtcaatc
     4621 tcttgtttat tacgcagatc aagagtacga gaaagccacc gatttattga aatttctaat
     4681 tgctcgaaca gagtatactc gctttgaata tattgaacgt ttagcaaaca tctatcatat
     4741 tatgaatgaa cctgtcaaag aatatcagtt attggacacc gtcttgtcag tagcagaact
     4801 gattgccctt cctgctggat tagaaaagaa actagtacgc cgccttttac gagtaaagca
     4861 acaactatcc gatcaagaaa aataacttgc gtttttttag gaaagtgcta tgattatttt
     4921 gttgaataaa tgggatgtag ccaaactggt aaggcacttg actctgaatc aagcaattgt
     4981 aggttcgaac cctgccatcc caatttgatg tgcttgctga aaaaatgaac cccctgtaaa
     5041 agaacaatct ttttgcaggg ggattattgt attattttta ctacttgtat ttagtttaca
     5101 taattaaatt atgaataata aaatcgggcg tattcttaat acaaaaactg atttacccaa
     5161 taattgctaa tgctttctcc ggaacttttt ctctaggaac ttcctcatac gtttctatgt
     5221 gtgctccttt attgctaatt ttcaaataat gattttcttt taacacagaa agaccattaa
     5281 attcaaccgt tcgttctttc ccattggcat cagcagcttt ctgcacatac cttgctgtac
     5341 catattcatt tacttcctct ggtttttgtg tctttacata gacctctcct ttttcaacta
     5401 atggatttag ctgatcaagt ataccaggga tttctccata acttccttca gtataatatt
     5461 tcagtccaaa tagcaaaata ccactaatcg ccgccagtcc aatcaaatat ttaatcactt
     5521 tcatctttct acctccttgt tatatattca gtatatgggt cagttaaaga aattccatcc
     5581 gtcccaagtt ggagattttt ctcctacttt agaagcgaga atcgagtgtt tttttagtaa
     5641 aaatgatcgt tatttgatat tattgacgtg tggataaatc agtttttctc gttttgtcca
     5701 tgtaaaaaat catgttgata gcaaaggatg tgttcaaaat gaatcaagaa gaattatttc
     5761 aaaaagtcaa ggaaatgatt aagaacggca atttcgatgg cgcaaaacga tttatcgaag
     5821 aacataaaga acaattagga ccttataaag agaaagccca aaatctttta aaagatgtaa
     5881 atatcgacag tgtcaaaaac aaatttaaaa atctattcaa ataagtaaaa ctttcacttt
     5941 tgtaaaatca aaggaggaca atgctgctgc atcgtcctcc tttggttctt ttctgaatac
     6001 ttcatactaa aataaacgtg tcggatcgta atgataagtc acattgccgt tcagtgaggc
     6061 ttttcccacc acatatttac gattctccaa cttcagccac gcttctcgaa aagccattaa
     6121 ttcttctctt tttacatcaa tcttttcgat ttttccatcg atcaaatctt gaataagttg
     6181 ttcatatgga ttcatttttt tcctccatct gctgtcaatc aataagttgt aaaaataggt
     6241 tcatatgctt ttttggtatc tagatcttct ctgactttga ctgtcgtata aataattttt
     6301 tctactggga tactataata acttccttca ccattattga atcggtgaac gtctttcttt
     6361 gctaaaatac catttatttt attcgtcagt acacgttgtt cgacactgtt acttacaaca
     6421 tagtaaatca cccgaatatt attgatcaaa tgcaaatcga tttctaacac tacatgatcc
     6481 atgaaaatcc cactccttct ttcactttga ttatagcaag tttctcctct aatttcaaaa
     6541 tcgatacaga ttttaccgga taaatccaca aaactgcttg cgccatttcg cacaaagtgt
     6601 tattttgtat catggacttt atttatcgga ggcaatagta tgatcgcgat cggtcaattt
     6661 gttttttata ttcctttttt tattatgata agcattcttt tctattacat aaaatggaca
     6721 aaaaagaagt tttcagtttt actggcctct ttacctgctg tatattttac ttatcaaatt
     6781 ttttctttta ggcattggga aacaacgtct gtattaataa cacacatcat cgaattgacg
     6841 ctttcagtaa tttttcttat tatttggatc tattttttat ataagaatca gaattgaatc
     6901 cctcgcatgt ttaaaaagaa aacgattaaa gatgcagcaa atttctgccg atctttaatc
     6961 gttttttcta ttctgttata ccaaaaaaat gtttgaatcg gtgaaggatc gttccttgtt
     7021 ttaattctat aaattcttgt gcgatcgggt acttttcttc caaattttct ttgttctttt
     7081 cgatcaggct tttcgcttct tcatattttt tatctgcaca tagtgctttt acttcctgta
     7141 atacttttcc tgcttccatt tgcctcaccc tcttgccatt ttattgcttt aagttttatt
     7201 taagttatac gcattattct agtttcatcc caaataacct tttttcattt ttactcctcg
     7261 aactaatcag ttttctaaag gctgattggt tcgtcttttt tatttttcct ccatttcatt
     7321 ctgctcttct gttaatttat ctaatgcttt gattgatgct gcggctcttg cataatcata
     7381 gctgtataat tccataaatt tatcaggcag aacaaaccac gctggatgat aaccgttagc
     7441 tgtgacaggg aatcctttat cttgttggtt atctccatcc catctcagag caacaccttt
     7501 ttttcctttc cactttacta aagcgatcga gtatcccttc tttgtttgtg tgccgtcttc
     7561 aataattgct aaaatttcta ttttttcttt tggcgcacgg acttcttgag gacgaaccat
     7621 tttctattcc tcccttatct cattacttca cgaccaacac actgcaaggg gcatgtgata
     7681 cgacataaga agcggtcgat ccaactaatg cttgttgtat agcacctttt cctgtcgctc
     7741 ccataacgat caaatcaata ttttcttgtc cagggatgac attggcaatc aaatttcttg
     7801 gatctccagt ttcaactatt gcgtggatct cagcaatgcc gtactctttg gcatcattga
     7861 tttttttcag catggctact tctgttcttt gtttttcatc tgcatagatt tttgaaaagg
     7921 cataggcact atgggtcagc tcaactttgt tgatgattga cacgataaat aagtgggctt
     7981 cattgtcctt tgctaagtca agggcttcag cgaacgcttt gatcgattgt cttgatccgt
     8041 caactgctac catgatgttc tggtatcttt tactcattgg aaccgcctcc tttttctttt
     8101 attgtaacat ctcaagcaaa aatacaaaaa atatacgcaa tatgtaaata aaatgatgaa
     8161 caaaagcaat ttctttcaag ttatttatac atttcttttc tcatcagaac acctgttcta
     8221 atcccattat ttctgcatta tgtgaaattc ctcatcattt ttcacatcga gcgaacatca
     8281 ttctgttaca aaaaacg
//
LOCUS       DACGLW010000102         8294 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.107, whole genome shotgun sequence.
ACCESSION   DACGLW010000102 DACGLW010000000
VERSION     DACGLW010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.107"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            434..601
                     /locus_tag="IXT53_002563"
     CDS             434..601
                     /locus_tag="IXT53_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ4906336.1"
                     /translation="MVYLNKKQTCEFLGIDNNTLDKWIRIYNFPVIKIDGIIRFDVED
                     IKGFMDTFKKI"
     gene            675..1901
                     /locus_tag="IXT53_002564"
     CDS             675..1901
                     /locus_tag="IXT53_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ4906337.1"
                     /translation="MPTKLSNGKYKTNLRYPKKFKEITGISSEKFQKTFPTRQLAIKA
                     ENEMKKKIAKVLREENANSLELKGQIKFKEFYKSKWLPRYELGQTTRSHKAPSDITII
                     NTKNLFRLHILPIFGEYAMSYLNANTEIISDELTKKSKEYANIKIIKCYVRSMFDIAE
                     VLNYIEFNRTTKIIQSITAPKKVALEEKRIREGKQALSSKELTDWLDAVRDDFNDQLL
                     SFHDYTLFMLTLYLGDRKSETYALQWKYIDFENQTVRLKYALDKHTKKKFTKGRKDTI
                     IQVPEIVMTLLQEWKSVQAEQLLKLKIKQTPDQYLFSYSKPSGEVNCPVHTDYLNYRI
                     NSIKKRHPHLVHLTPHKLRHTYATLARQGGADMNQISNALTHSGISTTKIYVNTPDLV
                     DKAVFEAFQRGLQKSN"
     gene            complement(2059..2676)
                     /locus_tag="IXT53_002565"
     CDS             complement(2059..2676)
                     /locus_tag="IXT53_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ4906338.1"
                     /translation="MAKIGYARVSSLDQNLDRQLELLSECDKIFSDKASGKDTNREGF
                     QEMMKFIREGDIVVVTELKRLGRNNKELTETMNLIQLKKATLEVLNLPALTGITDDNL
                     RRLLNNLIIELYKYQAEEERRYIRETQQQGIALAKAKGKYKGGTLKYRENDPRLQLAF
                     NLFLTGSTDKEVEELTGINRRTFRRYRMKHGITNEVREQKNKSFS"
     gene            complement(3359..4354)
                     /locus_tag="IXT53_002566"
     CDS             complement(3359..4354)
                     /locus_tag="IXT53_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family transcriptional regulator"
                     /protein_id="HAQ4906339.1"
                     /translation="MRPKLEDVAKRANVSKTTVSRVLNNRGYLSQKTIDNVYKAIEEL
                     NYQPNVVARQLFQKKTNIVGLLFPTVANPFFSELVEALEKKLYEIGYKVLIGNSMNNK
                     EKETNYLNQLLSDQVDGLIVGTHNQGIQEYKYQNLPIVAIDRVMNEDIPVVESDNYNG
                     GKLATKLLIAQGAKNIIHTNGPIDLQTPANRRRLAYEDTMKAYQLIPRTVTLDFNISY
                     VKKKQIFFQMFEDYPKIDGIFASNDIDAALILQVAKEKGLNVPADLLVVGYDGTLMTR
                     SILPDLTTVIQPINDIADTAVAILMKRINKEETKKEYILPVTLWMGKTSVKKKNN"
     gene            complement(4449..5321)
                     /locus_tag="IXT53_002567"
     CDS             complement(4449..5321)
                     /locus_tag="IXT53_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HAQ4906340.1"
                     /translation="MLIGSIEAGGTKFVCAVGDEDYRVQDQIICPTTTPRETLKKTVD
                     YFKQFDINALGIASFGPIEIRKNSPKSGYITSTPKVGWRNVNVLGYLQDELNVPMSWT
                     TDVNGSAYGEYIMSTLSNEKINSLVYYTVGTGVGGGAVVDGKLLGSFGHSEMGHTFVK
                     RHPDDIDFKGICPYHGDCLEGLVAGPTFEARFGKKGEDVSLTDPIWDVMAYYLAQAVI
                     QTTLIIRPDKIVFGGSVVNEKLLEKIRVNVNKLLDDYVEIPPLSKYITRPLIKNNGSA
                     TLGNFALGLNQLQE"
     gene            complement(5334..6761)
                     /locus_tag="IXT53_002568"
     CDS             complement(5334..6761)
                     /locus_tag="IXT53_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HAQ4906341.1"
                     /translation="MTKRAIELTNTRYRLGYHVSAPSGWINDPNGFCYFDGYYHVFYQ
                     HHPYSAEWGPMHWAHARSKDLVHWESLPLALTPGDQEDEGGCFSGSAIEKNGVLYLFY
                     TGHHYYGDNDPDHFWQNQNMAYSTDGIHFTKYEKNPVIAKAPEDNTHHFRDPKVWKHD
                     EKYYMVLGSQEKDGLGRAIVYQSLDLLNWDYIGPISKAKEVLTEGFMWECPDFFELNG
                     KSILLLSPQGIEANGKDYLNLYETGYFIGDYDYETAKFIRGNFYELDKGHDFYATQTT
                     LSPDGRRIVMAWMDMWESPMPEKVDGWAGALTLPRELELIGDRLYMKPVRELEQLRVG
                     SGVETTVNLAGEIIIAQDASFKEALIDVLLTKSKNQEVKFSLKTKDEELINIFYSKDK
                     NECTLTRNGKNDPRYGTIQMCDRLSLRVFIDKSSAEIFINDGELVFTERYYTENQPSM
                     HLSVSEEAFVSSTIYHLKKDAISYE"
     gene            complement(6815..8089)
                     /locus_tag="IXT53_002569"
     CDS             complement(6815..8089)
                     /locus_tag="IXT53_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ4906342.1"
                     /translation="MSKVNVFKNQSYLQSSATLLLFFASWGVWWSFFQLWLTSESNGL
                     GLSGSAVGTVFSANSLVTLILMFIYGTLQDKLYIKRNLLIFASVLATLVGPFFIWIYG
                     PLLDNNFNLGIIMGALFLSAGYLASVGVFEAVSERFSRLFGFEYGQARAWGSFGYALV
                     ALLAGFLFVKNPHLNFWAGSFFGSLLLLNLLFWNPKVEREANQNFNQEQAESNSIPSL
                     KEMFDLMKLPQLWTIIIFIVFTWTFYTVFDQQMFPGFYTGLFSTSANGEKIYGTLNAI
                     QVFCEALMMGIVPIIMRKLGVRNTLLLGVTIMCVRIGLCGFASTPLSVSCIKMLHALE
                     VPLFTLPMFRYFTLHFDTKLSATLYMIGFQIAAQIGQVILSTPLGILRDNVGYQPTFK
                     IISLIVLLAGIYAFFILKQDDRDVQGDPFIRG"
BASE COUNT         2747 a         1524 c         1185 g         2838 t
ORIGIN      
        1 tattttaaat ttttacatac ctgtttatga aaaactaaat aatctgtcaa aaaaatatga
       61 ttgttcattt ctaaatatgt tttccaattt taaaatcttt tttagcattt ttgtttcctt
      121 ttcatatggt ttttcgttgt taatagtgac taggatgata ttcctatttt gaccattgac
      181 aattaaatat aatttttgaa taacgttccc tgctattcga gctaagaatt caagtacgcc
      241 atgacctatt tagcgataac tacttagaga tttctttttg ttttgggaag acaaaatgcc
      301 atgacaatag cagtagataa tgatacttcc gtaaggctcg ccgtaaactg gggagaggtg
      361 aaattcctat gtgcttaccc aagtcaattc aaaaatttta gttcatttat aaggggatga
      421 ttttattaaa tcaatagtct atctaaacaa aaaacaaacc tgtgagtttt taggaattga
      481 taacaatact ttagataaat ggattcgtat atataatttt cccgtaataa aaatagacgg
      541 tataattcgc tttgatgtag aagatatcaa aggctttatg gatacgttca aaaagatttg
      601 aattttactt ttgactgcat atactaaaag tagcgaaata tactgcctta cttttttatt
      661 aaggagtgag gaagttgcct acgaagcttt caaatggaaa atataaaact aatttgagat
      721 atccaaaaaa atttaaagaa attacaggta tcagttcaga aaaattccaa aaaacatttc
      781 caactaggca attggctatt aaagccgaaa atgaaatgaa aaagaaaatc gcaaaagtct
      841 taagagagga gaatgcaaat tcattagagc tcaaaggtca aataaaattc aaggagtttt
      901 ataaatctaa atggcttcct agatatgagt tagggcaaac tactagatcc cataaagctc
      961 caagcgatat aaccatcatt aatacaaaga atttatttcg attgcatatt ctgcctatat
     1021 ttggtgagta tgccatgagc tatcttaatg caaataccga aataatatca gatgaactca
     1081 caaaaaagtc gaaggaatat gcaaacataa aaatcattaa atgctatgtt cgttccatgt
     1141 ttgatattgc agaggttcta aattatattg aatttaatcg tacaacaaaa ataattcaaa
     1201 gtattactgc acctaagaaa gttgctctgg aagaaaaaag aatacgagaa ggaaaacaag
     1261 ctttatcttc gaaagaactc actgattggt tagacgctgt aagagatgat ttcaatgatc
     1321 aattactttc ctttcacgat tacactcttt ttatgcttac tttatatctt ggggatagaa
     1381 agagtgagac ctatgctttg caatggaaat acattgattt tgaaaaccaa acagtccgtc
     1441 ttaaatatgc cttggataaa cacacaaaaa agaaatttac aaaaggtcga aaagatacta
     1501 ttatccaagt tccggaaatt gtaatgacac tattgcaaga atggaaatca gtgcaagctg
     1561 aacaactatt gaagttgaaa attaaacaga cacctgacca atatcttttt agttactcca
     1621 aaccttctgg tgaagtaaat tgtccagtcc atactgatta cttaaattat cgaattaatt
     1681 ctataaaaaa aagacatccc catttggttc atttaactcc tcataaactt cgacatactt
     1741 acgcaacttt agctagacaa ggtggggctg atatgaatca aatttctaac gctttgacac
     1801 attcaggtat ttcaactaca aaaatttatg tgaatacacc tgatttagtt gataaagctg
     1861 tgttcgaagc gtttcagcga ggattacaaa aatctaatta atgtgttcta gaatgtgttc
     1921 taaatataaa aaaaagacac tttccaaata attggaaagc gtcttgaacg ttgatatata
     1981 aaggatatta acgttttgag aattgagatg ctttacgagc tttcttaaga cctggtttct
     2041 tgcaaaatta tattagcctc aactgaaaga tttatttttt tgctccctaa cttcattagt
     2101 aataccatgt ttcattcgat atcttctaaa cgttctacga ttaatccctg ttagctcttc
     2161 aacttcttta tctgtagatc cagtcaaaaa caaattaaat gctaattgaa gtcgcggatc
     2221 attctcacga tattttagcg tacctccctt gtatttccct tttgctttag cgagtgcaat
     2281 tccttgttgt tgcgtttcgc gaatataccg acgttcttct tcggcctggt acttgtatag
     2341 ttcgataatt aagttattaa gtaatcgacg taaattatca tcagtgatac ctgttaacgc
     2401 tggcaaattc aaaacttcca gtgtagcttt cttcagctga attaaattca tcgtttctgt
     2461 tagctcttta ttatttcgtc caagtcgttt taattctgtc acaacgacaa tatcaccttc
     2521 acgtatgaat ttcatcattt cttgaaatcc ttctcgattg gtatctttcc cacttgcttt
     2581 atcactaaaa attttatcac actctgataa taattctaat tgtcggtcta aattttgatc
     2641 taagctgctt actcgagcat acccaatttt cgccataata aaactccttt aaaattctga
     2701 aatccggatc atttttaaat tctgttgccc aactctgttg tcatttttct agataaatgg
     2761 cataaatttt cagcgctaag tgacaaaagc aactaatttt tcatgataga tttttaaata
     2821 tctcaatcta tctccaaaat tagttgcttt tgtagctata gttgactatg aatatgacaa
     2881 ataattagaa aaaactgtgc caattaaata taaaaatgac actcatttac attcaataga
     2941 acaccaagag cccaacaggg tatactctgg tgtcctttta aaaaagtaac tttctgacct
     3001 aattaattga gctactaaaa aagctatgaa atgaggaata aaaaagggat caaaaacttt
     3061 taagtttctg acccttttga gttctaatta tttcttccag aacgaagaat gttatctgct
     3121 ttatctgcaa ttgattctgt tagaaggact gaaaaattta tgccagccct tttgcctagc
     3181 ttatccgccc attgaggaat tgttaaggtt tttctgacaa gttctatgtc tttcaaatat
     3241 ttggcaacat ctacacagat taaaagcatg attttgtatc atagtctatt ataagagatt
     3301 taacaatttt ttatatcacg aattaataat gaaattatca ttgatagtct ttataaattt
     3361 aattgttctt tttctttact gatgtcttcc ccatccataa tgtgacaggt aaaatatatt
     3421 ccttttttgt ttcttcttta tttattcgtt tcatcaaaat agcaacagct gtgtctgcta
     3481 tatcattaat tggttgtatt actgttgtca gatctggtaa tatactccga gtcattaaag
     3541 taccatcata tccgactact aataaatcag ctggtacatt taaccccttt tcttttgcta
     3601 cttgtaatat tagggccgca tcgatgtcat ttgaagcaaa aataccatct attttaggat
     3661 aatcctcaaa catttggaaa aaaatctgtt ttttctttac ataagaaata ttgaaatcta
     3721 atgtcacagt tcgtggaatt aactgatatg ctttcatagt atcttcatag gctagtctcc
     3781 gacgattagc cggagtctgt aagtcaattg gaccgtttgt atgaataata ttttttgcac
     3841 cttgtgcaat taataacttg gttgcaagtt ttccaccatt gtagttatcg gattcaacaa
     3901 ctggaatatc ttcattcatc actcgatcaa ttgcaacaat cggtaaattc tgatatttat
     3961 attcctgaat tccttggtta tgtgtaccaa caattaaacc atctacttga tcagacaata
     4021 actgatttaa ataatttgtt tccttttctt tattattcat cgaattgcct attaatacct
     4081 tataaccaat ttcatataac tttttttcca atgcctcaac gagttcacta aaaaaaggat
     4141 tggctactgt aggaaatagt aacccgacaa tgttagtttt cttttgaaat aattgacgcg
     4201 ctacaacgtt cggttgataa ttgagttctt ctatagcctt ataaacatta tcaatcgttt
     4261 tttgacttaa atagccacga ttgtttaata cacgtgaaac agttgttttt gatacatttg
     4321 ctcttttggc aacatcttct aattttgggc gcattctctt gtctctccta aataatgcaa
     4381 taattaacta tcgaaaaaaa gaactctata gaactactat acctgataat cgatattttt
     4441 tctatgtttt attcttgtaa ttgattcaag cccaaagcaa agtttcctag tgttgccgat
     4501 ccattatttt taattaaagg acgcgtaata tatttactta acggtggtat ttccacgtaa
     4561 tcatccaata atttattaac attaacacga attttttcta acaatttttc atttactacg
     4621 cttccaccaa atactatttt atccggtcgt ataatcaatg ttgtttgaat cacagcttgt
     4681 gctagataat aggccattac atcccaaata ggatccgtta aagaaacgtc ttcacccttt
     4741 tttccaaaac gtgcttcaaa cgttggtccc gcaactaagc cttctaaaca atcaccatga
     4801 taaggacaaa ttcctttaaa atcaatatca tcaggatggc gttttacaaa cgtatggccc
     4861 atttctgaat gaccaaacga tcctaatagt tttccgtcaa caacagctcc accaccaacg
     4921 ccagtaccaa cggtgtaata aactaatgaa tttatttttt cattcgataa agtggacatg
     4981 atgtactctc cataagctga tccgttcaca tccgttgtcc aagacattgg aacattcagc
     5041 tcatcttgta aataacctaa cacattaaca tttcgccatc ccacctttgg tgtagacgta
     5101 atatatcctg attttggtga attttttcga atttctattg gaccaaatga tgcaatacct
     5161 aatgcattta tatcaaactg tttaaaatag tcaacagttt ttttcaatgt ttccctgggg
     5221 gtggtcgttg gacaaattat ttggtcttgt actcggtaat cctcgtctcc cacggcacat
     5281 acaaatttag tcccacctgc ttctatgcta ccaattaaca tcataaaacc tccttactcg
     5341 taagaaattg catctttttt tagatgataa attgtactcg agacaaaagc ttcttcacta
     5401 acactcaaat gcatacttgg ttgattttct gtataataac gctctgtaaa aacaagctcg
     5461 ccatcattaa taaaaatctc tgccgaactt ttatcgataa acactcgaag tgataatcta
     5521 tcacacatct gaatggttcc ataccttgga tcatttttgc cgttacgagt taaggtacat
     5581 tcatttttat ccttagaata aaaaatattg attaactctt catcttttgt ttttaatgaa
     5641 aatttgactt cttgattttt tgatttagtc aatagtacat caattagagc ttctttgaat
     5701 gaagcatctt gagcgattat tatttctcct gccaaattaa ctgtcgtttc aacgccgctg
     5761 ccaactcgaa gttgttctaa ttctctcaca ggtttcatat acaaacgatc tccaattaac
     5821 tctaattctc gaggaagcgt taatgctcct gcccatccgt ctactttttc aggcataggg
     5881 ctttcccaca tatccatcca ggccataaca atacgtctac catctggaga taaagttgtt
     5941 tgtgttgcat agaaatcgtg tcccttatct aattcataaa aatttcctct aatgaatttt
     6001 gctgtctcat aatcataatc accgataaag tagcctgttt catatagatt taaataatct
     6061 tttccatttg cttcaattcc ctgaggggat aataaaagaa tagattttcc atttaattca
     6121 aaaaagtctg ggcattccca cataaatcct tctgtaagta cttccttcgc tttagaaatt
     6181 ggtccaatgt aatcccaatt taataaatct aacgactgat aaacaatagc acgacctaat
     6241 ccatcttttt cttgactacc caaaaccatg taatattttt catcgtgttt ccatactttt
     6301 ggatctctga aatgatgtgt gttatcttct ggtgcttttg ctattacagg atttttttca
     6361 tatttagtaa agtgaatgcc atccgtacta tacgccatgt tttgattttg ccaaaaatga
     6421 tctggatcat tgtctccata atagtgatgt cctgtataga acagatacaa cacaccattt
     6481 ttttcaatag cgcttcctga aaaacaacca ccttcatctt cttgatctcc aggagtcaaa
     6541 gcaagtggta aagattccca atgaactaaa tccttacttc tagcatgtgc ccagtgcatt
     6601 ggtccccatt cagcactata tggatgatgt tgataaaaca catgataata cccatcaaaa
     6661 tagcaaaatc catttggatc attaatccat cctgatggtg cagacacatg atatcccaaa
     6721 cgataacgtg tatttgttaa ttctatagct ctttttgtca taatatattc gctcactttc
     6781 tagataaaat tgttttttat tctgttggtt tccattatcc tcgaataaat ggatcccctt
     6841 gaacatctct atcatcttgt ttaagaataa agaatgcata tatgcctgct agtaatacaa
     6901 taagagaaat aattttaaat gttggttgat agccaacgtt gtctcttaat attcccaatg
     6961 gtgttgataa aatcacttgc ccaatttgag cagctatctg aaatcctatc atatagaggg
     7021 ttgctgatag ctttgtatca aaatgaagtg taaaatagcg aaacattggt aatgtaaata
     7081 atggtacttc taaagcatgc aacattttta tgcatgaaac agataatggt gtcgaggcaa
     7141 acccgcacaa tccaattcgt acacacataa tggttacacc taataacaaa gtatttcgaa
     7201 cccctaattt tctcataatg attggaacaa ttcccatcat taacgcttca caaaatactt
     7261 gaatagcatt caatgtccca tatatttttt caccattagc tgatgttgaa aacaaaccag
     7321 tataaaatcc cggaaacatt tgttgatcga ataccgtata aaatgtccat gtaaaaacaa
     7381 taaagatgat tatcgtccat aattgaggca gtttcattag atcaaacatt tcttttaaag
     7441 aaggaatact atttgattca gcttgttctt gattaaaatt ttgatttgct tcccgttcaa
     7501 ctttagggtt ccaaaataat aaatttaaca atagtaaaga accaaagaaa gatcccgccc
     7561 aaaagtttaa atgaggattt tttacaaata aaaatcctgc caaaagcgct accaaggcat
     7621 aaccaaatga tccccacgcc cttgcttgtc catattcaaa gccaaataaa cgactaaacc
     7681 tttctgacac agcttcaaaa actcctacag aagctaaata tccagctgac aaaaatagcg
     7741 ctcccataat aatgcctaaa ttaaaattgt tatctagcaa tggcccatat atccatataa
     7801 aaaatggtcc aacaagtgtc gctaatacag aagcaaaaat taataaattt cgtttaatat
     7861 acaatttatc ttgtaatgtt ccataaataa acatcaaaat taaggtaact aacgaatttg
     7921 ccgagaatac tgttcctaca gcactgccag ataaccctaa accatttgat tcagatgtta
     7981 gccaaagttg aaaaaatgac caccaaacac cccaagaagc aaaaaatagt aataatgtag
     8041 ctgaactttg taaataagat tgatttttaa atacgttaac ttttgacata tttttatcct
     8101 ccaacgatat atttgtatcc cctttcatga taaccggtta acatttttag aaaaggactt
     8161 gtactcctaa aattataaaa gaaatccgct tacatgtcaa ccggttattc tatttggata
     8221 atgccatata atgtcacagg atatgacttt tattggtgtg tcgcttttct tttttaatgg
     8281 cactattttt tata
//
LOCUS       DACGLW010000103         8228 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.108, whole genome shotgun sequence.
ACCESSION   DACGLW010000103 DACGLW010000000
VERSION     DACGLW010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8228
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.108"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            328..1251
                     /gene="truB"
                     /locus_tag="IXT53_002570"
     CDS             328..1251
                     /gene="truB"
                     /locus_tag="IXT53_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HAQ4906343.1"
                     /translation="MDGLLTLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEEKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVKRFKRVDITDEVWQKVKNGMRLNYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            1255..2196
                     /gene="ribF"
                     /locus_tag="IXT53_002571"
     CDS             1255..2196
                     /gene="ribF"
                     /locus_tag="IXT53_002571"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HAQ4906344.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGEIWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            2591..3376
                     /locus_tag="IXT53_002572"
     CDS             2591..3376
                     /locus_tag="IXT53_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ4906345.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWINNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENDKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            3389..4240
                     /locus_tag="IXT53_002573"
     CDS             3389..4240
                     /locus_tag="IXT53_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HAQ4906346.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(4364..4816)
                     /locus_tag="IXT53_002574"
     CDS             complement(4364..4816)
                     /locus_tag="IXT53_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ4906347.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQIREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNHVVNFSQWINEKLLMQTDISLVS"
     gene            complement(5281..5649)
                     /locus_tag="IXT53_002575"
     CDS             complement(5281..5649)
                     /locus_tag="IXT53_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HAQ4906348.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            5799..6281
                     /locus_tag="IXT53_002576"
     CDS             5799..6281
                     /locus_tag="IXT53_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HAQ4906349.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            6318..7328
                     /locus_tag="IXT53_002577"
     CDS             6318..7328
                     /locus_tag="IXT53_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HAQ4906350.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPPWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQPSEKYQKEWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTISHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(7443..8156)
                     /locus_tag="IXT53_002578"
     CDS             complement(7443..8156)
                     /locus_tag="IXT53_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906351.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIKQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLINVIVMDN"
BASE COUNT         2784 a         1337 c         1667 g         2440 t
ORIGIN      
        1 ttaagaaact gaaaaaataa ataaaaaaaa acggaaaaaa gactggaaga atggacagtc
       61 ttttttttcg tttttgggaa gtgaaaacca aactatttac ctgtaagcca cccataaatc
      121 caatcaaatg gtgcaaagaa aaaatcttcc attctattcc ctcctttaaa aaaaacataa
      181 catgatttct atttacgtga gcatgaattt tgttacgttt ttattgccga aaaataaaaa
      241 ccgttcatta tgatgtcaaa ttcgtcagta attgatgcaa tgtgttataa taattcagtt
      301 aaatcaaaaa tgaaatggag aaaagacatg gacgggttat taactttatg gaaagaacgc
      361 ggaatgacaa gtcatgattg cgtattcaaa ttaagaaaaa tcttacatac gaaaaaaatc
      421 ggacatgggg gtacgcttga cccagacgta gacggtgtgt tgcccatttg tattggaaaa
      481 gccacaaaag ttatcgaata tttgacagat tctgggaaaa tctatgtagg cgagataact
      541 ctaggtttca gtacaacgac ggaagatcga tccggagaac gggtagaaac aaaaccagta
      601 gatatgtctt tatctgaaga aaaaatagat gaagtgatgg cttcatttat tggtgagatc
      661 acccagatac caccaatgta ctcagcggta aaagtcaacg gtaagcggtt atatgaatat
      721 gcccgcaata atgaaacagt ggagcgtcct gttagaaagg ctcagattta ccgttttatc
      781 cggacaagtg aattgacaag agacgaagtg acaggaacca tttcttggcg cttcgaagta
      841 gaatgcggga aaggaaccta tgtccgcaca ttagctgtag atacaggcaa gaaacttggt
      901 taccctgcgc acatgtctga tctgacccga acagcaagtg ccggtatgtc ccagaaagat
      961 gctctgacat tagaagaagt agctcaagct gtgaaaaatg ggacaatcga tgattgtttg
     1021 ctgccaatag aaaaaggagt gaaacgattc aaacgagtag atattactga tgaagtatgg
     1081 cagaaagtaa aaaacggtat gagacttaat tatactgtat ttgcgctccc agaaatgccg
     1141 tcagaagaaa ttgctttgtt ttataaaggc aaagttgtca gtatctatca gccaaacccg
     1201 aaagaaaaaa acaaattaaa acctagtaaa gtattgagaa atgaggaata acgaatgaat
     1261 atcatcaaga tcagacatcc ttaccaatca gaacaaattc cagaagacga tgtcgtttta
     1321 gtactaggat tttttgacgg cgtgcatatt ggacaccaga aagtcataaa gacaggtatg
     1381 gaaattgcac aaaaagaagg attgaaatta gcattgatga ctttcaatca gcatccatcg
     1441 attgtcttta aaaagatcga tcctagcagc gtgaaatatt tgactagcct taagcaaaaa
     1501 gaagaaaaaa tggccgcttt gggtatagat tatttatatg aagttgattt tacttcttct
     1561 tttgctcatt tagcaccgca ggaatttgtt gatcaataca tcgtagggtt acatgcaaaa
     1621 tatgctgttt ccggttttga ttatacgtat ggaccaaaag acattgcaga tgtcgctcat
     1681 tttcctgaat atgccaaagg aagattcaaa atcgtcacag tttctaaaga agaagaacaa
     1741 ggtgaaaaaa tcagctctac ccgtattcga ggattactag acagcggaca agtaaaagaa
     1801 gcagcaaaac tattaggttg cacatatgag gtagaagggg tagttgtcca tggcgatgca
     1861 cgaggcagac tactaggatt ccctacagca aatgtcaaag taaagagcac agttcatttg
     1921 cctaaagaag gtgtttatgt atcggaaata aaaattgggg aaatttggta tccagccatg
     1981 ggttcaattg gacataatga tacattcggt gaaggacggg agctgactgt agaattgtat
     2041 attttggact ttaatcaaga tatctatggt gaacatgtcg cagtacgctg gaatcaattt
     2101 ttaagagatc aagtgaaatt ttctagtgta gaggaattga ttgaacaact caaagaggat
     2161 gaacaagcaa cagctgaata tttttctcag caataaaaca ttatgcataa aatctcttgt
     2221 ttatgtataa ctagcaattt caataaaatg ggtgataaat aagcccagga aaggggctgt
     2281 gacaaaagtc ttgtcacagc ctttttttcc aaataaatgg tttttcagaa gtagcttctt
     2341 cggaaataag ccgaaatttc acaaaaattt gagaagtaat ttttgtaaat tacttcttat
     2401 ttcttgaagc tggccacttc tgtcacaacc tctttcttta tagaaataaa aggacactag
     2461 caattcatga aaacgattgc taaagcgtat catttgcttt agactatata tcttggtgta
     2521 cactcacttt aagacggaaa taaaagttat ccgtaataac aggtttaggt atgaaaaagg
     2581 agtgggtact atgtataagg aattagaaaa caaagtcgca gtagtaactg gtggctcaaa
     2641 ggggattgga acagcgattg ctcaacgatt cgggaaagaa aaaatgaatg tagttgtcaa
     2701 ctataactcg gataaagaag gagcagaaaa agcagtcaaa gcaatcgaag aggcaggcgg
     2761 aaaagcagtc tctgtacaag cagatgtcag ttctgaagaa ggaatacagc gtttacttga
     2821 tacggcttta gaaaattttg ggacactgga cgtttggatt aataatgctg ggatggaaaa
     2881 tcagcatcca acccatgaac ttagccttga agattgggaa agagtattaa aagtcaattt
     2941 gactggcgtt tttatcggaa cgaaagcggc tttaaattac tttacagaga atgataaaaa
     3001 aggaaatatc atcaatatgt cttctgttca tgaacaaatc ccatggccaa cgtttgctca
     3061 ttacgctgct tcaaaaggcg gagtaaaact attcaccgaa acggtagcaa tggagtacgc
     3121 accaaaggga atcagaatca atagtatagg acctggtgct atccgtacac ctattaatgc
     3181 ggcaaaattt gatgatcctg aacaaaaaga actcttggaa agtatgatcc cactaggacg
     3241 cgtaggtgaa ccaaaagaaa tagcggcgac agctgcatgg ttggcttcag acgaatccag
     3301 ttatgttaca ggtattacac tatttgcaga tggtgggatg actttatatc cttctttcca
     3361 aggtggaaaa ggataagggt gaataaaaat gggtttattg attgcattga tcccggcagt
     3421 tgggtggggg atccagccac tgattttgaa aaagattggt ggcagaccaa caaatgagat
     3481 attaggaact ggtattggag ccgtgctcgt tggcttactt gttcaattat tcgcttctcc
     3541 agctggtatt tcagtgacta catttttgat tagtttactt tctggtgcat tctgggtcat
     3601 cggacaaaca ggacagtata catctttcaa cttgattggt gtttctaaaa cgatgccaat
     3661 ttcgactgca ttacagttag tcggaacctc attgattggt gtcttcgctt ttggagaatg
     3721 gtctggaagt atgggaaaaa tcattggtgc agcagcaatc attcttcttg tatttggttc
     3781 tgctttaacg gcaatcagtg atgatcctgg aaaaaaaggc ggtatcacaa aaggaatcag
     3841 tctattagca tcaacaagta ttggttattg ggtttacagt gcattgccca aactagttga
     3901 tgcaagcgga ctttccattt tctttccgca aatgatcggt gtctttttag gggcagtgat
     3961 ttatgtgatt ttccgaaaac caaccgcatt ttctgaagac aagagttgga aagcaacaat
     4021 agttggagcc atcttcagtg tgtcagcatt ggcttatatt ttttcagcaa atgaaaatgg
     4081 ggttgctact gcatatatca ttacacagct aaacgtagtt atctctacat taggcggtat
     4141 gtacatcctg catgaaaata agacaccaaa agaaaaaaaa ttaacgatta tcggattatt
     4201 gctgatcgta gtaggaagca tggtcacggt ctttatttga ggataaagat ggtaaaatct
     4261 catttattaa aaaaatattt gtttgaacca aacagtaagt acttataaaa aaatagcgct
     4321 agaatttcca acatctctgg aatttctagc gctttttaat tgtttaagat acaagtgaaa
     4381 tatctgtctg catcaataat ttttcattga tccattgact aaaatttacg acatgattga
     4441 agttaccacc gattagtatc tccgcatgat tgtctttata aagatcgtat tgtaaaagac
     4501 ggtctttttt atttgcgtaa acatcgttat ggtactggac taattgttct cggatttgat
     4561 tgattgctgt aaaatcacga tactgaaggt tgaaacgcat cgtgattggt gcatttagca
     4621 tgaatcgctg catttctttt gctgcttcct ctgcttgatc tccgtatagg aactcaaaaa
     4681 gattacgttc agaaaatttc cattgcccgc caacctttgt tccttttagt tttccttcat
     4741 gaagataatt tctgatagaa cgtgtagtaa cacctaattg ttcggctaat tcatttactg
     4801 agtaaaaatc tttcaatgtt caccctccta tgtgaatggt tctactgact tagttgatat
     4861 ttgcttaatc acgaaatgta gaaagaacga ttttaattaa ggtcattgta aaaatcaagt
     4921 cagaaaattc taacttttct ttctctaaag aaattcgttc tctcctaaag tgatattata
     4981 tttaaatata cctgaaaata atgggcagta ttatgaaaga tcactgatat tttctcgtat
     5041 tttttcctta ttcaaaagtt ttgcaggaaa attgctgaaa aaataatttt actaagtgga
     5101 aaattcaaag ccattcaatc agtgattttt ttcagcacaa gaaagactcc ttataaaaat
     5161 agcagatact ttgtcaatta agtgtttaac cagggatgat aaaaaggata ggattgaaca
     5221 aaacctgtat aagctgatga atagtttcct cgccagtaaa aggtttattc gtataaagct
     5281 ttatcgccaa aatttatcat gacctttttt atcttcaata ttaaatgaaa tcaattgttc
     5341 aatcaattcc cagttgatta cacttttcca aggaactttg atgacgttat ccgtatatac
     5401 gtagcctgct tcttggatat ttttttcaag ggcacgaatc gctgcttttt ccggtgcgat
     5461 agaaaaatga tttttagcaa aactaaatgc aataataaag gtaccatggt cagtaaacat
     5521 aggttggttc cattttacag ttgtgtctag ctggggatac gtttctgtta tcttagcaaa
     5581 aaggtctttg atcttttgtt gatgttcttt ttctttgatc tgattgatga tcggctgaaa
     5641 tttttccatt ctaaaacgcc tccattaagt ggtttgattt attttaccag aaaaacaata
     5701 gttatgtata aaaaacgaac aaaacacaag taatgttgta accgctatat ttatatgata
     5761 agatgtctca aataataaaa tagaaaagag acataaaaat gattgacttg gcaaagaaat
     5821 tcttacacga aaatatatat tttgagttgt atcatcatcg agccatttat acaaatgaag
     5881 atgctttggt aataaagaaa gaacaaggat ttacaggtac agagaccaaa agcttattct
     5941 taaaagataa aaaagataat aactacgtat atctgacttt tactacgaaa caaacggatt
     6001 tcaaagcatt agggaagatc gtcggtaaaa ggctttctat tgtttcatca gaacgcatgg
     6061 aagaacaaac gggacaaaag cagggcgctg tttctccgtt tggttatgaa aaacagattc
     6121 cgattattat tgatgaagag ctcttatcaa atgaaaaact tgtttttgca ccaggtagac
     6181 cagatcaaac gatggtcatc caaacggttg acttaaacaa aatcatccag ctacttgaga
     6241 tgccgatatt tttgatgccg gcaaacgaaa aaaacaatta accaagtaaa atagtgcata
     6301 cttggaggtg aagcaacatg ataagttatc cattattaga aagaaatgca acgattgggg
     6361 ttactgcacc atcctcaggc gtatcagagg aacaccattc gttactcgaa tcagctttaa
     6421 agagaatgga agaaaaatct ttccaagtag ttactggaaa gacgctttgg acgcaagata
     6481 aggaaaaatc aggtccagca agaaaacgag cagatgaatt caatcaaatg atgcaagatg
     6541 aaaatatcca aatgatcttt ccgccttggg gaggagaatt attgattgag attctcgatt
     6601 ttgtcgattt tgatgctcta caaccaaaat ggatagtagg ttattcagat attagtacgt
     6661 tgctacttgc tgtcacatta aaaacaggga tagccactgc acatggaacc aaccttattg
     6721 atttaagagg agagtacttg gatgaaacga cagcaatgtg ggaaagtgta ctgaaaacaa
     6781 gagaaggaga atcgattcta cagcaacctt ctgaaaagta tcaaaaagag tggccccatg
     6841 agcattcatc atcttctgtt ttccatctca ctgaaccaac aaattggcaa tcagtcagcg
     6901 gagatgcagt aaggatgaag ggacgattat taggtggctg tattgatacg atcagtcatt
     6961 taatcggtac cccttatgga gatgtccaat cttttaaaag aaagtataat gatggaaagc
     7021 cgatcttgtg gtttctagaa aattgtgagc tatcgtcagt tgatttaaga agatcacttg
     7081 ttcagatgaa actggcagga tggtttgatg gcagctcggg gatcatgttc ggtagaagtg
     7141 cagcagagga tttgttagat tataaaatga aggatgtata tgaagatctg tcaaaggaat
     7201 taaaactacc gatcttgtat gatattgatt gtggtcatgt accgccacaa ctgacattga
     7261 tcaatggtgc ctatgcagaa gtagcggtag aagatagaaa agcgactgtt ttgcaaacgt
     7321 ttatttaaaa aattttttcc tgccttggag tttatcaaag accaacaaat ctaaatagaa
     7381 aagaaaatga aagaagctga cagtattttg ccagcttctt tttgtatgat ctattagaaa
     7441 ttttaattat ccataactat cacattgatc agctgtttca catcatttgg attacctgta
     7501 ggaatagcta ctacagatgc attatcaata atttcttgag ttgtttctct aatgaacgct
     7561 ggataagatg tttgatctgc aggcatttgc caatgtgaag gaactggata actagtgatt
     7621 gtcccgtcat ggttacttga atacactaaa actccctggt aactgtattt cccagtgagc
     7681 ataaccacat ctttcggata gactgcactc cctgcatcat aaggggcgat aggtgttcct
     7741 gcaggataat gaataacatg tagttcgaaa tcacccgatg tatctctaaa agtattacct
     7801 ctaattgcta gccaaacacg tgcgtactca atttgtagat cagaatatcc tgctaacgga
     7861 tctattgact gagaagactg tgaggaagtt gtgtctattg ttgaagaaga aatatttgtc
     7921 tcaactgtac tactagatgt agaagaagta ttgggctctg agactttact gcttgttgaa
     7981 gatgtattcg tttcagtaga agctgatttc tgagtacttg atttttcagc tgaactggtt
     8041 tgtttgattg aacttttgtc tgtggtcgca gatggatcac tattttcttt actaggagac
     8101 gtacatccta caaggaataa agagaccaac aataaaataa aacctaactt tttcatataa
     8161 aatacctcat tattttttta ataccagtat agcataatcc tgaatttttg aatccgtaaa
     8221 accttgtc
//
LOCUS       DACGLW010000104         8071 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.109, whole genome shotgun sequence.
ACCESSION   DACGLW010000104 DACGLW010000000
VERSION     DACGLW010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8071
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.109"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            complement(127..675)
                     /gene="aac(6')"
                     /locus_tag="IXT53_002579"
     CDS             complement(127..675)
                     /gene="aac(6')"
                     /locus_tag="IXT53_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAQ4906352.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            1228..2331
                     /locus_tag="IXT53_002580"
     CDS             1228..2331
                     /locus_tag="IXT53_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ4906353.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            complement(2524..3591)
                     /gene="rlmN"
                     /locus_tag="IXT53_002581"
     CDS             complement(2524..3591)
                     /gene="rlmN"
                     /locus_tag="IXT53_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAQ4906354.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(3821..6268)
                     /locus_tag="IXT53_002582"
     CDS             complement(3821..6268)
                     /locus_tag="IXT53_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAQ4906355.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            complement(6457..7893)
                     /locus_tag="IXT53_002583"
     CDS             complement(6457..7893)
                     /locus_tag="IXT53_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ4906356.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
BASE COUNT         2244 a         1746 c         1378 g         2703 t
ORIGIN      
        1 gtacacggtg ttccaaaagt agcttcttcg gaaataagtt ggaatttcac aaaaattcaa
       61 gaagtaattt tcgtaaattc cttcttattt cttgaagctg attacttctg tcacaacctc
      121 tttttattat tgagaatttg gtcgaggaat aatcgttttt gccatccaaa tatccggttt
      181 gtcccagcca tttgcatttg gtaagacacc tacgatttta taacctaatt tttcatagaa
      241 ttcatacgga tgttcacgaa ggttctggat ggaagccact ttatcaaatg tatgctcata
      301 caggtccgtt tgacttaacg ttgttccatg gtctaaatca tccgtaccta aataaatcgt
      361 gattcctcct ctggaagcta cttctttttc taagtaattg actaatcgag tacctatttg
      421 gttctttcgt cgggagcttt ctacaactaa tggatgcaat tcccaacctg tgataccgta
      481 ttgagggatt gcaccaataa atcctactaa ctcatcttgg tctaccgctg ctaccgcgat
      541 tcgttctgga ttcatcattt cttctacttc ttctgccgag ctgtctccat attcttccgg
      601 ccaagtcagt ctcagtaaat cagaaagctg atctttcaat actggattat tacggtcaaa
      661 ttcactgatt atcatacgtc ttcccccttg ttcttgctta ttctaacagt ccttttgaaa
      721 aaacgaaatg ttctcgctca aaaatcttta gctaccaatc agtatctcta ttctttcatt
      781 atttatctta cttataaaat aaaaaaatta tacagaaaca ctttattcaa aataggaaag
      841 atcaggaaag ccatcaattg gaggaaaaaa ctctgtttgt tcatcatgat atagattatc
      901 ttttgcttta aaaaaaacca attatgaatt gacaaataaa acctgttttc atataataat
      961 cttaattttt agaattttta gataattaca aaaaagcaaa gggaatcggt agaaatacta
     1021 agaggaacaa aaattcatta ttgtcgtttt caatgttttt gtacttgttc gtttttagga
     1081 tacttattga tgatttcaag gtgttcatgc caccttattc atcacttatc ctgtcttagg
     1141 ttcacctcta ctccatctac agccgcagtc agatcgatca ctctttaaaa ttttaacccg
     1201 tttcaacgaa gaaagaagga aatgaatatg agtcaaagtg ttttcttacc aaattatacc
     1261 attggaacag atgcgtatga aaaagcaaat ggaatcgtca aagaatatgg gacaaaagta
     1321 gtgctgattg gagggaagac tgcactttca aaggcagaaa acaaaataaa agtagctttg
     1381 acacaggaaa tagaggttgt cgccagtctc tggtacgggg gtaattcaac ttatggaaat
     1441 attgaacagt tgatccagca tgaaagtgtc caaaaagcag acgtcctgtt tgcagtcggt
     1501 ggtggccgtg cttgcgatac tgtaaaagtc attggtgaaa aactagctaa accagtcttc
     1561 acctttccca ctattggttc gaactgttcg cccgtcactg cggtatgtgt catctataac
     1621 gaagaaggcg taatggacgg actcttcttt ccttcaaaac cacctgtaca caccttcatt
     1681 gatacagcaa ttattgccga agcacctgat aaatacttat gggcaggtat tggcgatgct
     1741 ttatccaaag aagtcgaatc tactttttct gctcgtggcg atgatctagg ctacacggat
     1801 agattaggaa tccaaatcgg aaaaatgtgc attgatcgtc tcttgactta tggtacgcaa
     1861 gcattgaaag atagtgccga cgatcgtccc tcatatgcaa ttgaacaagt cgtttcggat
     1921 atcattgtta ctaccggatt ggtttctgtt ttagttaaca ataactataa ttctgctatg
     1981 gcacactcat tctattacgg tacgactgtt ctagaaaaaa tggagggata ccttcacgga
     2041 gcatctgtca gttatggtgt actgacctta ttgatgatgg atcagcagtt cgatttgttt
     2101 gataaaatct atgtgtttat gaaaaagaac caacttccag tatcgttaac cgacatgaat
     2161 ctcaatgtca cgaccgattt agaagcaatc ttagataaag ccatggcaac aaatgatatt
     2221 aaacatgtgc cttatgaaat cacccgagaa aaatttaaac aggccattct tgacgtagaa
     2281 acttatcatg ctacacaaga aagccagcaa ggatataaga aaatcagtta aataattgct
     2341 aaaaccccca tcaagcaggc tgtcatcaaa ttgaggacat ttctttgctt gatgagggtt
     2401 tttcttgtca aaaaaagctt tatagctttc gctatagaat cttttctctt atttaagttg
     2461 aacagttcct taattaagaa aactgctcct tttatggtaa ttgaaaaaac gagtaggata
     2521 cgattatttg gcaactgttt ttttcatttg cttgcttcgc aattgtccgc aagctgcatc
     2581 aatatctgta ccatgttctt tacgtatcac acagtttacg ccgtttttct tcaatacatc
     2641 ataaaatctc aatacatcag cttttggact gcgagaatat tgatcatgtt cactgactgg
     2701 attatatgga atcaagttga catatgctaa ttttttctta tcttttaata gatctgctaa
     2761 ttgttgcgca tgctctggtc tatcatttac ttgattcaac atgatatatt caaacgtgat
     2821 tcgacggttt gttttctcta aatactcatc cacagcttcc atcaatttct caattgggaa
     2881 acttcggttg atccgcatga tcgatgttcg cacctcatta ttcggcgcgt gcaaggaaat
     2941 agccagattg acttgcaagc cgttattagc gaattccttg attttgtgtg ctaatccact
     3001 agtggaaact gtaatgtgcc gagcaccaat tgccaatcct ttggcatcat tgatgatatg
     3061 caaaaagtcc attacattgt cgtaattatc aaatggctca ccgatcccca ttacaacgac
     3121 atgtgaaacg cgttcgccaa gattgcgctc atcaaaataa tgttggacca tcatgatctg
     3181 tgcaacaatt tctccagcag tcagatcacg gttctttttg agcagcccgc ttgcacaaaa
     3241 cgtacagccg atattacatc ctacttgtgt cgtcacacaa actgacaagc catattcttg
     3301 tctcatcagt acagtctcaa tcatgttttt atcagggagt tcaaacaaat attttactgt
     3361 accatccgaa gcttcttgga cgaccatctg ttttagagga ttgatgataa aatgctccga
     3421 taatttttcg atcaattgtt tcgatagatt cgtcatttct gtgaaattac ttacacgttt
     3481 ttgatacaac cattcccaaa cttgggcagc tcggaatttt ttctcgccgt tttctataaa
     3541 ccaattgatt aattcttcat tcgtcaagcc ataaatggac tgtttttcca aagtattttc
     3601 cctctttttc tcaaagtttc gctacatact atacatgatt catttcctta atgcaatact
     3661 tcacaaataa atcgaataga actttaatct cacaaaaact aaaaagacaa gacatcatac
     3721 cgtattagaa aagatggaaa aaaaccattt tttcttttgg tatcatcatg ccttgtcttt
     3781 ttattggtta ttcgtttttc aacccttcat cacacaagtt ttacatgtca ttatcgtccg
     3841 gcaacgtttc tgatgtttct tcctgctcaa tgaatacttt ccgaggcttg cttccttctg
     3901 acgggccaac aactccatga gcttccagtt catctactaa acgtgctgct cggttgtaac
     3961 cgatccgaaa tcttctttgg agaagggaaa tacttgccgt ttgcatttca acgacaagtg
     4021 cttttgcttc ttcaaacagc tcatcttgtg gctgttctgg agcacctgtc gtttcaactt
     4081 catctgtcgg catcattttc tcttcataat gagcttcttg ctgatctgta acaaattgaa
     4141 cgacccgttc tacttcgtgg tcagaaatga atgctccttg cacacggatc ggtttattct
     4201 cacccattgg caagaatagc atatctcctc gccctaagag cttttctgcc ccattagaat
     4261 caatgatcgt ccgagaatct gtaccgcttg ataccgcaaa tgccattctg gaaggtacat
     4321 ttgctttgat gattcctgtg ataacatcca cacttggacg ttgagtagct aggatcatat
     4381 gaatccccgc tgcccgagcc atttgcgcta aacgaataat cgcatcttca acttcattgc
     4441 tggctaccat catcaaatca gctaattcat ctacgatcac tacgatgaat ggtaaggttg
     4501 gatgtttcgt tccgttttcc aagtttttct gttgaacaaa ttcattgtaa ccagaaatat
     4561 ttcttactcc ggttgccgcg aatttttcat atcgttcttc catttcttgt actacttttt
     4621 gtaaggcttg cgctgctttt ctagggttcg tcacgaccgg cgtcaacaga tgaggtatgc
     4681 cgttgtacat attcaattca accattttcg gatcgatcat catcagtttt acttcatggg
     4741 gcttagcacg cattaatata cttgtgataa tcccattaat tgctactgac ttcccactac
     4801 cagtcgaacc agccacgagt aagtgaggca tcttcgaaag atcagccgtt tgtacccgtc
     4861 cagaaacatc acgtcctaaa ggtacttcta gtaatttatc tgggtggttt ggttgtgctt
     4921 cgatgatttc tctaaaggaa accatactga tctttccatt tggtacttca ataccaatca
     4981 gtgatttacc tggaatcggt gcttccatcc ggacatcttt cgccgctaaa gcaagcgcga
     5041 tatcatctgt taagttcaca attttactta cttttacccc tacagccggc tggacttcga
     5101 attttgtgac ggatggtcct aagctggctt tgacgacctt ggcatcgaca ccgaaacttt
     5161 ggaaagtttg ttctaatacg ccgatatttt tttcaatttt tttatactca tcactttgat
     5221 ccacagttgg tatagaatca agcaaatcaa ctgtcggtaa ttcatagtct tgattttcct
     5281 gttctgcttc aatctcaaat tctaatgagg taccgtcatc ctctggcaac tcatctgaaa
     5341 aaccttctgc tgtattggtc tgttttggtg tatctggtac tggtttctct agattcgcag
     5401 gatggatatt ttcttgaaaa ctgtcgattg gaacaaaact tagctgctcc ggctctgtat
     5461 tttctggttc ctgccattca tcagcagtat tttccctttg ctggctcacc gcttttttct
     5521 tctcatattc tgcagcttct ttttctgcag cttcttttgc agctaagcgc cttgcttctt
     5581 tcgcttctgc gcgctgtttc attctctctt cctttgccgc ttgctttcga gcttgcttgg
     5641 caggatcacc ttccaataat tcctgtactc gttctccaat tgattggata ccatttaaca
     5701 gctgatagcc ttccagatta ctgaacagga atgctcctcc tgccagtaat agcactgctg
     5761 ctaaataact tccaggctgt gcaatcaaga agtaagtaaa atgataaagg attgctccaa
     5821 tcatcccacc accaacgtta ttgtctactt gactttgttt tatatcactg gctaacaaat
     5881 cccatgtcgt ccccatgata tttggttccc cagtatggag tttgccaaat aaatgggcat
     5941 gtaacaataa taagataccg aaataaaaga gtatgccgcc aaaccagcgt cggtttttac
     6001 caattgaaaa ctctgtattc ttaatgacaa tccaaaatcc taatacggct aataaaagac
     6061 agaggatttg gtaagtattt cctgcaatga tccgaaagcc gtttgcaatc aatattccta
     6121 aaaaacctaa ttttaaaaag ccgaagacag caaataaaat caaggcaaac ccaacaccaa
     6181 tcgtcagcat ttgttcctgc tgctgttttt gctttttcgt ttttttcgtc tttttccttt
     6241 ttttagtagg ccgtttcttt tgtgccattt attatccctc atttcatcaa gtttcattat
     6301 acaaaaaaaa gtaagggaac taaagttttg tttgaaagaa ctgacgcttt ctttcatttt
     6361 tcagcttatt ttacagtttt tcttaatttc taaatgattt tatcaagaaa aggcacggcc
     6421 atatccagcc gtaccttctt gttttaagta tgtgaactaa aggttctctc cattttcttc
     6481 aatcagtttt tggtaccagt aataactgtc tttcttatat cgttccaaag atccttcttg
     6541 tgtttcgtct tgatcaacat atacaaatcc atatcgcttt tgataaccgt tcagccagct
     6601 aagcaaatct gtataagacc aggtgcaata gcctaggact tctgctccat ccgaaattgc
     6661 ttctttgatg gctttgacgt ggctttgtaa gtaattgatt cgatagtcgt catggatttg
     6721 tttatcttct gtcaacttgt catactctcc taagccattt tccgtaatca gtaccggcat
     6781 gcggtaacgg cttgtgatac gacgcagtcc gatccttaat ccttctgggt caatctccca
     6841 gtcccaattt gttcgttcga caaatggatt ctcaactttt ttatacattc caggaactcc
     6901 agtttcttct gatgaacctt tttttcctgt cgtattcatt tttccagcac caactccgtt
     6961 taatggattg aatgcattcg tcgccgtttg atagtaattg atcccaagaa aatctggttt
     7021 agcagattct agcaattctg catcgccagc ttcaatcgtt ggtgcccatc ctttttcttc
     7081 taagaacctc atggcagcaa tcggatattt tccccacata tagacatcca tccaataatt
     7141 ggccatcagg tcttctgcat tttcagcagc cagaacattt tcaggattac tgtcaattgg
     7201 ataatttggc gtatacgcaa agcttggtcc gatttttcca ggcatttcta actcgtgaaa
     7261 tttattgatg acagatgcat ttgctaaatt agcaatatgg ttaacttcat acattcgttt
     7321 cgggtctgtc acacctggcg gatgtgctgc taacaaataa cctaatgaag tgaagatatt
     7381 ttgttcattc aaactaatcc agtaatggac tttcccgcgg aatgcctcaa ataatgtttc
     7441 tgcataatga gtgaaatctt caatgatctg acgtgattcc caaccattat attcatcttg
     7501 caatgcttgt ggaagatccc aatgatataa agtcaacata ggttcaatat cgttttcaat
     7561 caattcgtcg atcaatctct cataaaaagc gagcccagct tgattgactt ctcctcgtcc
     7621 attaggaaaa atccgtgtcc aagctacaga gaagcggtaa gctttcagac cttgttcttt
     7681 cattaaagca atatcttctt tataacgatg atagtggtct actgccaaat caccgttcgt
     7741 tcctttaaat gtctttccag gtatccgtac aaatcgatcc cagactgatt ctcctttccc
     7801 atcttcttgc caagctcctt ctacttgata cgctgcagaa gcagagcccc acagaaaatc
     7861 ttttggaaat gaggtaagtt ctttgtgttc cattcatctc ttcctttcct ataaccttca
     7921 atcgttcctc ttttagaata acacgaaacc ccttccacaa caaaataaaa atatgtaaag
     7981 gaggttgtga aaaagtcgct tagtttcgag tagtaagaaa aattttgtga aaatggcttt
     8041 ctacattttt gcataaattt ggcttaatac c
//
LOCUS       DACGLW010000105         8066 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.110, whole genome shotgun sequence.
ACCESSION   DACGLW010000105 DACGLW010000000
VERSION     DACGLW010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.110"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            <1..883
                     /locus_tag="IXT53_002584"
     CDS             <1..883
                     /locus_tag="IXT53_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HAQ4906357.1"
                     /translation="ISVPVALFSFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTY
                     NDYFQIGMIGMIVSLVVVLTVANISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVL
                     GVVLLDLQIILGFILAGIWALLFTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMT
                     LAMFNNSAAYASPYFSAIFGDWIPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILA
                     VVLAVNPTMSPAFLFPLFAITTIAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGW
                     IIGAILCLITFLLYGNA"
     gene            931..3060
                     /locus_tag="IXT53_002585"
     CDS             931..3060
                     /locus_tag="IXT53_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HAQ4906358.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKNLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKAIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPHTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            3035..3724
                     /locus_tag="IXT53_002586"
     CDS             3035..3724
                     /locus_tag="IXT53_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906359.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKEIE
                     KRCTEYDLDYK"
     gene            complement(4132..4817)
                     /locus_tag="IXT53_002587"
                     /pseudo
     CDS             complement(4132..4817)
                     /locus_tag="IXT53_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(4953..5147)
                     /locus_tag="IXT53_002588"
     CDS             complement(4953..5147)
                     /locus_tag="IXT53_002588"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906360.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(5197..5637)
                     /locus_tag="IXT53_002589"
     CDS             complement(5197..5637)
                     /locus_tag="IXT53_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HAQ4906361.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELEQLFQAYASLDDVNS"
     gene            complement(5641..6486)
                     /locus_tag="IXT53_002590"
     CDS             complement(5641..6486)
                     /locus_tag="IXT53_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HAQ4906362.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVDWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLVANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAVYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            6635..8053
                     /gene="pepV"
                     /locus_tag="IXT53_002591"
     CDS             6635..8053
                     /gene="pepV"
                     /locus_tag="IXT53_002591"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HAQ4906363.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
BASE COUNT         2480 a         1515 c         1713 g         2358 t
ORIGIN      
        1 tatttcagta ccggttgcac tgttctcatt tatgggctcg attatggctg gaatctttgc
       61 taatatcgtc aatttcaaac aatatcaaac gatttatgct ggtttcaatc cagcggctga
      121 aagctatact tataatgact atttccaaat tggtatgatt gggatgattg taagcttagt
      181 tgtggtacta acggtcgcta atatatcaat gaacaaaaag aaacgctatg caatggcagc
      241 aaacgtacca gctgaaggtg gcgacgcacc aatgatctca tggttagccg tattattacc
      301 agttttaggg gtcgttcttt tagatcttca aatcatctta ggttttattc tagcaggtat
      361 ctgggcattg ctgtttactg gaaaattgcg cggaggttat aaagaaatct gtcgtcagtt
      421 tgcgaaatta tttacagatg gcgcagtcga tgttgcacca atggttggtt tcttgatgac
      481 cttagcgatg ttcaataatt cagctgcgta tgcttctcct tatttttcag ctatttttgg
      541 agattggata ccgcaatcac cattagtctt ggcaatcgta tttgctatat tgacaccact
      601 aggatttttc cgcggtccaa tgaatctagt cggttcaggc tcagctattt tagctgtcgt
      661 gttagctgtt aatccaacga tgtcgccagc ctttttgttc ccgttatttg caattacaac
      721 gattgctcct cagcatttag atattacaca atcttgggtt gcttggggat taggttatac
      781 aaaagtaact tctcgagaat atatgaagaa atccatccca actggttgga tcatcggtgc
      841 gatcttatgc ctgatcacgt tcttattgta cgggaacgcc tagtaaacaa aacgtcgata
      901 aaaacgatta gataaatgga ggaaaagaaa atgaaacgtt ttgtgcgaat gggaattgat
      961 gttggcggta cacatacgaa agcagtcgca atcgataatg cgacgcacga aatcatcgga
     1021 aaatcttcag tgaaaacaac ccacgatgat gtccgtggag tagctgcagg tgtcgttcag
     1081 tcttttcaaa attgtttgcg ggaaaataat atcagtccag aagatgttgt gtttgttgca
     1141 cattctacca cacaagcgac taatgcactg attgaaggag acgtagcaaa agtcggcgta
     1201 atcgggatgg caaaaggtgg attagaaggt tttttagcaa aacgacaaac tagattgaac
     1261 gatatcgatt tagggaataa gaaaaaaata gagattgtca atgcattctt acctgtcaaa
     1321 aacttaaatg tagaccgtgt ttcagaaacg atttcttctt tagaaagaga aagagcggag
     1381 gtcttggttt cttctatggc atttggagta gataatgggg aaccagaacg agttgtttat
     1441 gaagcagcat ctgtgaaagc gatccctaca acgatggctt ctgacatcac aaaattatat
     1501 ggattgacaa gaagaactcg gacagcagca atcaacgcat ctattttacc aaaaatgcta
     1561 gatacggcaa cctcgacaga agactccgta cgagaagcag gggtcaatgt ttctttgatg
     1621 attatgcgcg gagacggcgg agtaatggaa atcaatgaaa tgaaaaaacg acctgtttta
     1681 accatgcttt ccggtcctgc tgcatcagtc atgggctctt tgatgtactt gagagcgtct
     1741 aatggtgttt attttgaagt aggcggaaca acaaccaata tcggggtcat taaaaatgga
     1801 cgtccagcaa tcgattattc aattgtaggt ggccacccaa catatatctc ttcattagat
     1861 gttcgggttc taggtgtggc aggcggttcg atggtacggg ctaatcagtc aggaatcatc
     1921 gatgtcggtc cacgttctgc acatattgcc ggtctagatt atgcagtatt tacggagaca
     1981 gaaaaaatca aaggtcctaa ggttgaattt ttctctccta aagaaggaga ccctgctgat
     2041 tacgtcaagg tagtgatgga agacggagaa gaagtgacta ttacaaatac atgtgcagcc
     2101 aatgttcttg gattagtgca agaagaacat ttttcttatg gaaatgtccc atctgcacga
     2161 aaagccatac aggcattagc agactattgt catacgacgg ttgaagacat cgcagagcag
     2221 atcatggaaa aatcctatgc taagattgaa ccggtgattt tagaattggc agacaagtat
     2281 cacttggaga aagatcagat ttctcttgta ggtgtagggg gcggcgctgc ctcattgatt
     2341 acttacttta gtaataaaat gggtgtgaaa tattcgattc ctgaaaacgc cgaagtgatt
     2401 tcttctattg gtgtagcact tgcaatggtc cgagatgttg tagaacgtat catcccttcg
     2461 ccaagtaaag aagatatacg atcgttgaaa aatgaagcga tgaataaagc gattgaatca
     2521 ggcgctacac cagaatcgat cgaagtccac gtagaaatcg atccacatac ttcaaaagta
     2581 acggctatcg ctactggttc tacggaagtg aaagcaaccg atctaaccaa ggagatcaca
     2641 acagaagaag cactagagct tgctgcagag gatatgcgat taaataaaaa tgaagtttgc
     2701 ttattggaaa atacaccttt cttttatgta tgcggcgaac agaatcgttc aaaaaatgct
     2761 ggaagtcttc ggatcatcga tcaaaaagga tttatcaagg tccaacgtgg acatgcttca
     2821 tgtatgaaaa caactgcagc caattatatg acagctgtcg aacaactttg ggaagatatg
     2881 gcagtttatc aaactgaatt gattgcccga ccagaatttt atctctgctt aggagcaaga
     2941 gtctctgatt ttacagcgac ggatctggaa caactacagc ttttgatgga tctggaagta
     3001 tctactatgg aaccggagga agaagtgatc gtcgttgccg gaaatatcaa acaaacataa
     3061 gttagaagag ttactaaacg aattacaaca gatccccgaa gaaatttggg gattctacca
     3121 gtttcggcga gatttatttt ggaaaaagat tcctttgtcc aagcagaaaa ttcttataca
     3181 acaatcaatc gattgtggta tcgaaacggc atgtagcatc aaaaaaaagt acccttttgc
     3241 cgatgtagga gaaatctgtg aacaaatggc tgttccaatc gtttcctgtg aatcagagca
     3301 aataaacgaa cgaatcactt ttgcaactta tgcagaagat gaagggatcc gattgatgac
     3361 ggagccgtta gaaaaattaa aatgttcagg actagcttcg atctctaaag aaacagcgca
     3421 agctttgatt attggacatg aactatttca ccacatcgaa gcttctgtta agggaattta
     3481 tacgcaaagc gaaaaaattg tgctgtggcg gttgcctttt tatacacatc aatcaaacat
     3541 tcgtgcattg agcgagatag cagcgatgag tttttcaaaa gagatgaatc aaagcgattt
     3601 ttcgccttat gtattagagg cagtcttact ttggccatat aatgaaattc gtagccaagg
     3661 gatcttagaa gaaataaaag aaatagaaaa aagatgtacg gagtatgatt tagattacaa
     3721 ataaagagac tgtgacaaaa gtcgtgtcac agtccttttt ttccgaataa acggtgttct
     3781 cctgtcaact cctcggtcac aagtcaaaat attcaacaaa catgagaagc tgttttttga
     3841 aattgttcct tgtgactcgg agatgaacgg caggctcacg acctcttgga aaaaggtgtt
     3901 caaaagcttg catctgcggt aataagaaca gccaagttaa aaacataaga agctattttt
     3961 acttggctgt tcttattact tgatgcaggg cagctttttc acaacctctc aataaacggt
     4021 gtgcaatcag ctgaccctcg gtattagctc aaaaaaaacg gcaaaatatg aggaactatt
     4081 ttgccgttta tttctctaat actcaggtca aaacgctgat tccaaaacct cttatttgat
     4141 gggtctgtcc tgaatatctt caaaataatc ggggtaatta tgaatcacgt attcttgctc
     4201 ctcgataatc atatgtaaat gcatcgtgca aagaaatccg acttcatcaa agttatgatc
     4261 aatcattgat tgaagcattg tctgatgttc atctaatact cgaccccagt ctaattctga
     4321 aatcgtcaaa cgtaaccatc tgaaacggtt caagtgcgta ctataacttt ctagccaatc
     4381 ccaaatttct cgttttccag caatttcata acaagttcgg tgaaaatcag tatccaaatc
     4441 aaaaaagctt ttttattttg agcgatcact gcctgatcgg tttgttctag tattttttct
     4501 agtttagctt gatcttgctt ggtcattttc gcactgcatt cttgcaggat cggtttttcc
     4561 aacttttccc gcgtataacg ggctaatgaa gcagatcgga tatcgatttt tgaaacataa
     4621 gtgccggact gtggaatggt ttttacaagc tcttgattgc gcagttggat cagtgcttct
     4681 cgaatcggtg tacgcccgat agctagttgt tcttctaaca ctttatcgga gattttactt
     4741 ccgggtgcta actcggaata aatgatttgt ttacgtatag attcataagc ttgctgttgc
     4801 aaattattga taaccattta accttccttt ctatgcagat ataatctatt ataacataaa
     4861 tcgcttacaa atcgtgtggg cataaaaaca cagtctgcaa aaaaagccct gataggcatg
     4921 cctattagag cctcgaaaag aaaaatattg aatcaatctg gtcgtacctc ttggatcggt
     4981 ttgacacgac cgccgattct tttagcaaaa gcttcggatt cttttttgtc tttgaaaatg
     5041 agcagattgt ctaagttttt ttcccgagtg cgacagccag ttttatcgat gtaaccgtgc
     5101 attactttaa cgacatacat ggggatcacc tgcttttttc ttttcattat acgaactact
     5161 cgacacaata caagaaaaat gcttagacgg tctttattaa gaatttacat catccaaaga
     5221 agcgtacgct tggaataact gttctaactc cggattgact ttgctcagct tcactaatcg
     5281 ttgcgaatca gaaagtaaaa aacagtgttt actactgcca gtagttttta attttttgtc
     5341 ttcagttccc acaatttcgt agctaaaatc caaccgtgtt cccgtatact tttggatagt
     5401 tgggtggatg ctcacttttt ctccgtaatg gatcatttct ttatagctgc aatttacttc
     5461 cagaacgggg atgatgatcc cagattcttc gattttttta tagggaaacc ctagatgtac
     5521 caataaggct acccgagcct cttcaaacca gcggatataa ttgctgtgat gaataatccc
     5581 catttgatct gtttcataat aaaaaggttc tcgcacataa cctgtaaatt tctccatgac
     5641 tcatgcctcg ttttccattg attttttaca aaaataattc atccacttcg gaagtgcttc
     5701 gctgatttgt gtcggcaaaa caatatattg ttcttttgcc agctggtctc caatgaaact
     5761 atgaagataa acagctgctg caatcgtttg gtcattttga ggaaactggg ctaaaaatcc
     5821 tgtgatcatc ccagctaaag tgtcacccat gccgcctgtt gccatagcag cagaacctaa
     5881 aggattggtg tagaagtgct ggtgaccata aatttttgtc tcatgacttt tcaaaacgag
     5941 cacagcaccg atttcctgtt gcttcttttt gttttcttta tcccgttgtt cttgtatcgg
     6001 tagagaactc aaccgttgcc actccatttg atgaggtgta agtacagttt gttcgggata
     6061 gggtagcaaa agatgattag caacaagtaa acttaaagca gagccatcaa tcaccagcca
     6121 ttgttctttt gattggtttt ctagtacttg tttgaaacgt tccaaactct tagaagaagt
     6181 acccaatccg ggaccaatca agagtacatc tgcttgcttt attacggaaa gaaactggtc
     6241 agtatccgtc caatctacaa tcattgcttc tggcagatga ctgtgaagag cactccgatt
     6301 tttttcatca gttactacag tcgtgagacc cgctccactg tgtacggcag ctaaagcact
     6361 cataatgatt gctccgccat attgttcatt gccgccaatc aaaacgatac gtccaaatgt
     6421 tcctttatgt gaaatctttg gacgaggtgt gatgactctt ttcactagtt gttcgtttat
     6481 cagcatcgaa aaacctcctt ttactcatta tagcgtatat tttttttaga gaaaaacaga
     6541 taagaaaagg aattgtcaga ttttttgaac tatttttttc tgtaatttgt ggtatcatga
     6601 acacgaagac ttaaataagt ggaggattaa ctttatgaca attgattggc aaaaagaagt
     6661 agaagcacgt aaagaagact tgcttgaaga cttgaaaaat cttttacgtg tcaacagcga
     6721 gcgcgatgat tcaaaagtaa caccagatgc accatttgga cctggacctc gcgacgcatt
     6781 gaaacatatg ttagcttatg gcgaacgtga tggatttaaa gtaaaaaatg tcgataacta
     6841 tgcagggcat attgatcttg gcgaaggaga cgaaacgcta gggatatttg gacacatgga
     6901 tgtcgttcct gcaggagatg gctgggacac agatccttat gaaccagtaa tcaaggatgg
     6961 aaagatcttt gcacgcggat caagcgatga caaaggtcca agtatggcag cttactatgc
     7021 gatgaagatc attaaagaac ttgacttgaa actatcaaaa aaagttcgct tcgtagtcgg
     7081 cagcgatgaa gaaagcggct ggggcgatat ggcttattat ttcgaacatg aagaagaacc
     7141 agattttggt ttctcaccag atgctgaatt cccaatcatc aatggtgaaa aaggaaatgt
     7201 ttctttagct ttacgcttca aaggcgacaa tgcaggagat tatgtcttga aatcttttgt
     7261 ttccggactt cgcgaaaata tggtgccagg aacagcaact gcagcacttc aagtaccaag
     7321 tgcagatgca gctatcgcga tggaagaagc attttaccaa ttcatcgaag caaatccagt
     7381 aagcggaacg attgaagcgg acaatacgta tgtaaaaatc gaattagttg gtaaaggcgc
     7441 acatggtgca agcccacaat ctggtatcaa tgcaggttct ttcttggcat tgttcttaga
     7501 taactatgaa ttcctaggat ctgctaaaca atttatccat gtagcagctg cttatgtcca
     7561 tgaagatttt tatggagaaa aactaggagt cgcttatgaa gatgaaaaaa tgggcaaatt
     7621 gacaatgaat gctggactat ttgcttttga agaaaatggt acagaggaag cgaacttcat
     7681 caatatgaat ttccgttttc caaaaggtgt aacagtagac ggattgcaaa gtgacatcga
     7741 acaaacagtg gggcaagaag gcgcaacagt tactcgaggt gctcgcgtaa tggaaccaca
     7801 ttacgtacca atggatgatc cattggtggc tacattattg caagtctatg aagaccacac
     7861 tggagaaaaa ggctacgaac aaatcatcgg tggcggaact tacggacgtt tgttgaaacg
     7921 tggcgttgct tatggagcga tgttcccagg atatactgat acaatgcatc aagcaaacga
     7981 atttatgagt cttgatgact tgttcagagc aacagcaatc tatgcagatg ccatctatcg
     8041 tttggcgaaa taaaaatact aaaaaa
//
LOCUS       DACGLW010000106         7760 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.112, whole genome shotgun sequence.
ACCESSION   DACGLW010000106 DACGLW010000000
VERSION     DACGLW010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7760)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7760)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7760
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.112"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            complement(552..1898)
                     /gene="gor"
                     /locus_tag="IXT53_002592"
     CDS             complement(552..1898)
                     /gene="gor"
                     /locus_tag="IXT53_002592"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HAQ4906364.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(2032..3273)
                     /locus_tag="IXT53_002593"
     CDS             complement(2032..3273)
                     /locus_tag="IXT53_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAQ4906365.1"
                     /translation="MTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAYGLRS
                     RAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISAVIVS
                     DPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVAEIRK
                     NTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFGQERN
                     SLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNVYKAA
                     VDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVYKFVG
                     EVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRAPNPM
                     MILTMPVAQPVKPGDMIRKRK"
     gene            complement(3291..4214)
                     /locus_tag="IXT53_002594"
     CDS             complement(3291..4214)
                     /locus_tag="IXT53_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HAQ4906366.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(4435..6927)
                     /locus_tag="IXT53_002595"
     CDS             complement(4435..6927)
                     /locus_tag="IXT53_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HAQ4906367.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(6934..7401)
                     /locus_tag="IXT53_002596"
     CDS             complement(6934..7401)
                     /locus_tag="IXT53_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HAQ4906368.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(7537..7610)
                     /locus_tag="IXT53_002597"
     tRNA            complement(7537..7610)
                     /locus_tag="IXT53_002597"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7574..7576),aa:Arg,seq:acg)
     gene            complement(7668..7741)
                     /locus_tag="IXT53_002598"
     tRNA            complement(7668..7741)
                     /locus_tag="IXT53_002598"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7705..7707),aa:Pro,seq:tgg)
BASE COUNT         2161 a         1691 c         1247 g         2661 t
ORIGIN      
        1 aaatcagtgt tttgtcctga gtattagaga aacaaatgga aaaatagttc ctcatatttt
       61 gccgtttttt gagctaatac cgaaggacag ctgattgaac actatttgtt cggaaaaagt
      121 gactaactat gacacaattt ttttcacagt cccttttttc acttgatttg attcgtacca
      181 gcgaacttga cgagaacttt cttggtttgg taactatatg gtattctttt aacatcctcg
      241 tttgcttctt ctaatttatt tatacaaagt cacacttttt taaacatctg ttcttcgata
      301 catttctatt tcttttaaac atcgatacca atattttgaa cttaagagtg cataccaaat
      361 ggattcattt actcttcgtc agttacgaag ataatcaaaa tctccaactt ccctacttat
      421 ttcgttttga gaattatcga agcaatctta cataaataac taaaaaacaa aaaaccagaa
      481 aatcagtata ctgattttct ggttttacat ggtttgttca ataaaaagct gttggaactg
      541 ttaatattaa attatcgcat cgttacgaat tcttccgctc ctgttggatg gatggctact
      601 gtattgtcaa agtcttcttt agttgcaccc atcttgatag ccacggcaaa accttgcagc
      661 atctcatcga caccaacacc aatagcatgt aagccgacga ttctttcttc ttttccgaca
      721 cagatcaatt tcatttcgca tttttggcga taatcattta atgcaaagta cattggggta
      781 aagcgtgaac gataaacttt caattcattt tcgccatatt tttccaatgc ttcttcttct
      841 gtcaaaccaa ttgttgcaat tggtggatgc gtaaagacga ctgtcggaac aagtttatag
      901 tcaagataag aatctttctt tccattaaat aaacgttctg ataaacgtct accagctgcg
      961 attgcaacag gagtcagatc taatttaccg atcacatcac caacagcaaa aatatgttca
     1021 tccgttgtat tttggtattt atctactttg ataaaccctt ttttgtccaa ttccacattt
     1081 gtattttcca gacctaattt ttcggtattc ggtacacgtc caccggcaaa aagtacacaa
     1141 tcaccagtta aggtttcacc attttcaaat agcaccgtat attcgtcatt gtttttagta
     1201 atttctttag caatatgatg tgcgtaagtt tttattccag cttcttggta catttggatc
     1261 aaattgtccg aaagcatttt atcaaacgtt ctaagtggtc gttcttttcg atatgcccac
     1321 atgacttcac tacctaaacc acgaaataca cctgaaagtt cagccgcaat gtatccagca
     1381 cctaaaacga ttgctctctt aggtaactct gttaattcaa aaaatccatt tgaatcaatt
     1441 gcgtattctc caccaggaat ggtcatttta cttgcacggc ctccagtagc gatcaagatt
     1501 ttaggagcac gataggtttc accgtttact tcgatcgttt gactatcgat aaaagtagcg
     1561 taacctttta tagcctcgac atgattgcta tctaatcctc tttgataagc accgtgtaga
     1621 aaatcaatat atttttctcg tctttctact agaccggcga aatcaacatt ttttaaatct
     1681 gcttggatac cgtaacttga ggcatctcgt tcaattgttt ccaaaatcgt acttgcttgc
     1741 cacataactt ttttaggtac acaaccgacg ttgacacatg ttccgcccaa ttcattcgct
     1801 tcaatcagca aaacttttgc gccatgcatc cctgctctgt ttgctgaagc aataccgcca
     1861 gagccgccac caattacgat gtaatcatat atttgcattt tgttcctcct tattgaagag
     1921 atcaatcaaa acctttattt aacgatcctt tttctacaga aaacaagtgt aacacttttc
     1981 ttgaattttg tatgcgaaga tgcttaaaac agggatatat cagctttttc cttatttacg
     2041 ttttcggatc atatctccag gtttcaccgg ctgtgcgact ggcatagtta aaatcatcat
     2101 tggatttggt gctcggtcaa tggattcgcc ttcttcatta tgcataactt caacggtttg
     2161 actgaaatga ctgaatcctg gaccataaaa ttcgatttca tctcctacag aaaacaagtt
     2221 tctctgacgt atcgtagcaa tctgtgttgc ctcatcataa gataatactt caccgacaaa
     2281 tttatatact gggattttac gacgttcacc gaataattgt tcgttttcag ttggtgtatt
     2341 gtagtaaaaa cctgtagcta attctcgttg tgctactttc cataactcgt cgatccattc
     2401 ttgcttgcag acatagtttt caggatcttc catataactg tctacagccg ctttatagac
     2461 atttgacacc gtagatacat aatggatcga tttcattctt ccttcgatct tgaagctgtc
     2521 tacgccgttt tcaatcaatt ctggaatatg ttcgatcatt gacatatcca ctgcactcat
     2581 cgagaattct tcttccactt cgcctgtatc agttaaactg tttcgttctt gaccaaaagg
     2641 catatcgaac aatccatact tccagcgaca agattgagaa catccgccac ggtttgcatc
     2701 ccgcatcgac atatggttgg acaatgtgca tcgaccagaa taagagatac acatggcacc
     2761 atggataaat gcttcgattt cggtatccgt atttttacga atctctgcca cttcctccat
     2821 tgaaacttca cgagctaaaa cgacacgttc caacccttct tctttccaga attccaatgt
     2881 ctcaaaatta gttgctgaag cttgagtgga taaatggatc ggcagacctg gggcttctgt
     2941 cgcacaaatt tcgatcaaag ctggatcaga aacaatcact gcagaaatac cgacatcacg
     3001 caaactgcgg aaaaattccc ctgctccatc ttcatttcct tcatgggtga ccatattagc
     3061 agcaacgtat accttcgcat catgttcttt tgcaaaagca actccttcag ccatttcttc
     3121 ataagtaaaa ttacctgcac ggcttcttaa accatatgca ttcccgccaa tatatactgc
     3181 atctgctcca taatggatcg cggtttttaa cttttccagt gttcctgctg gcgctaggat
     3241 ttctggtcgt ttcaattttc tttttactgt catttctttc atcatactcc ttatttgata
     3301 gcagaaggat caatataaaa gaatcctgta tctaattcac ggttttcagg atgcaattta
     3361 ctgatttcgc tagtcatttt ttccgctttt tcttgatccc agctaccttc taaaatttgt
     3421 ttttttgctt cggcgaatag tcgagtgatg tttacgaatt tttcgcctgg agtaaataat
     3481 ccgtccaatt tccatgtaca gtaatgatgc tgataaagtt cattcaattc attcgagaga
     3541 ttcagatcat tgctggcaaa aatatgtgtg ccatgactat cttcataaat agaataatgt
     3601 gtctcttctt ttttaggttc cgagataaaa agacctcttt cacgatcttt ttgttcgtct
     3661 tgtttcgtat agttatagta attctgtagc agtggacgtt tggattgatg gatgcatgta
     3721 gcaccataca caagggtttc tacaggaata tcgagttttt cttccatcgc cttcatctct
     3781 tcaaaaggaa cttcacgagc aagtactgct ccaactgcgc cttttttcgc ccagaaattg
     3841 atttgacgtg cgctcgttac taatgtttca gcatcataaa tatacggtat acgcaattca
     3901 ggattttttt tcatcgtata aataatacct gtatcaccaa ctgtgatcac atctacgttt
     3961 atttctttta aaaaagccaa atattcaggt acatttttca ttttttcagg atgaatgatc
     4021 ccatttacag caatattcgc ttttttacca tacttgtgtg ctaattctac caattgtcgc
     4081 tgttcttctc gtgtaaatga agcaggcaga cgcaatccaa attgctcttc accgaaatat
     4141 agtgtatctg cacctgcagc aagcaactgt tctgcttgtt caatagtttc tactgtggtt
     4201 atgagttcga tcataatttt ctcctcctac aacttcataa tagtagcata gctctaaaaa
     4261 ctttccaata cttcttctat ttaaagtcgg atttttgaaa attatttcac aaaaaatcaa
     4321 aataaagttc gatatattct gtccaaatat aaaaagagat tgtgacaaaa atcttgtcac
     4381 aacctcttaa tcaagtctta ctattataaa aattcagcta cacaggccaa gtaactatag
     4441 ggaacctact gtttcctttt taggtccgcg aacattcaat gtgatcttac cttttgaagc
     4501 acctactgtt acgtggtcac ctaaacgaat ttctccgcca agcaaagcct cgctcaatcg
     4561 atcttcaatt tctttttgaa gtgcgcgacg gattggacgt gcaccatatt caggatcaaa
     4621 tccagctttc ccgatcacat cgattgcaga tggtgtgatt ttcacttgaa tatcttgttc
     4681 tttcaatcgt ttgataatcg ctttgctcat gatttggacg attgtatgga tttcatcttt
     4741 actcaatgaa tggaagacga cagtctcatc gattcgattc aagaattcag gtcggaaagc
     4801 tttctttaac tcttctaaga tacgtttttg catggctttg tgatctttag taacatcttg
     4861 gacattgaat ccgacatttt tttcttctcg aatttgtgtc gctccgatat ttgaagtcat
     4921 aatcatgatt gtatttctaa agtccacttt tctaccttta gaatctgtta aatgaccatc
     4981 atccaatact tgcagaagta aattgaaaac gtctggatgt gctttctcca cttcatctaa
     5041 gagaatcact gaatatggtt tttgacgaat tttttctgtc aactgtccac cttcatcata
     5101 ccctacgtat cctggaggag acccgattaa tcggcttgta ctgtattttt ccataaattc
     5161 tgacatatca acacggatca gggcatcttc acttccaaac atgacttctg aaagtgcttt
     5221 ggccaattct gtttttccga caccagttgg tccaaggaac atgaatgaac caattggacg
     5281 atcaggatct ttcaatccgc ttcgtgcacg gcggatcgca cgtgaaactg ctttgacagc
     5341 ctcatcttga ccaacgacac gttcatgcag aagtccttcc agttctaaca agcgttcgct
     5401 ttcttttttc tccaactgct gtagagggac accagtccac tccgatacaa ctgtagcaac
     5461 atcttcttct gttacacgat cagaataacc ttgctcctgt tttgcttcaa ttgcagaaac
     5521 tttttgcagc tttttacgta gtgctttttc ttgtctgcgt aattgagcag cattttcaaa
     5581 gtcttgtttt tggatcgctg cttccttttc ttgtactagt tcatcgattt ctagtttgat
     5641 cacagttgat tttgttaagt gatcggtttg atccaaacgg actttcgctg ctgattcatc
     5701 gatcaaatca attgctttat cgggtagttg acgagaagtg atataacgaa cagaaagatt
     5761 gactgctgct cgtacagctt catctgtgat ctccactcca tggtgctctt catatcttgt
     5821 acgtaatcct tgaagaatca cttctgcttc ttcaggagtt ggctcatcta cttgaatgcg
     5881 agcaaaacgt cgctctaatg cagaatcttt ttcgatatat ttttgatatt catctagggt
     5941 cgttgctccg atcgtttgta attctcctcg agctaacgcc ggtttcaaga tattcgaagc
     6001 atcaattgct ccttctgctc cacctgcacc aattaaggta tgcaattcat caatgaataa
     6061 gattacttga ccatcattat agatttcgtc gatgaccttt ttcattcgat cttcaaattc
     6121 accgcgatat ttcgtaccag caaccaaaga tcccatatca agcatcatca ggcgcttttc
     6181 ttgcatgtct tctggcactt cacctaaaat gattttttga gctaaccctt cagcaatggc
     6241 tgttttgcct acaccaggtt cacccaccag tacagggtta ttctttgtcc gtcggctaag
     6301 aatctggata agccgtttca cttctctcga acgaccaaca actgggtcca gcttgttttc
     6361 ttttgctaat ttcgtcaaat cacgtgctaa tgaatctagc gtaggtgttc cttgcggtgt
     6421 ttgccgttta tcttgtacat ttacatttct acgacgattc attccttttc cagcagaatc
     6481 accagctagc cccatttttt gtttcagaac ttgacgggct tttgaaaggc tgatacctaa
     6541 gttgatcatg attcgagtag ccatgatact ttcatctttt aataagctca tcaaaatatg
     6601 ttctgttcca actttttgag aaccagtttt cttcacttcg ctgctagcta aagcaaaaac
     6661 ttgtttcatt cttggtgaat atggaagaaa gactccttta gggtaagaac ggataccacc
     6721 ataaccaatc agatgttcaa tttcttcata aatttccgtt tcattcaaat ccatttcacg
     6781 taatactttt ccagcaattc cattttgctc gaccactaag gctagtaaaa catgttctga
     6841 actaactgat tgatgtttca accgttttgc ttcttcttgt gcaatttcta atactgcttg
     6901 ggctctttca gtaaaaagtt cttccatcta atctcacttc ccatcttcat aacttaaacg
     6961 ttccagaaat gctcggagaa tccgagcacg tgtatgatct tcatattcat tgaaattcaa
     7021 tgtgtttttt gcaattgcac tcaacatcaa gtttccttct ttttttgtaa ttatttggtc
     7081 ttcataaagt ttttgaataa tagcaaaagc atttttctca ctgattgtat catcaaataa
     7141 ctgatttatc tgctcaagta attgtttttt atctgaaatc cttacttttg caattcgtat
     7201 ataaccacca ccaccacgtt tgctttctac tagataacca cgttggatgg taaaacgggt
     7261 attaatcaca tagttgattt gtgaaggaac acagttaaag aggtctgcca tttcaatcct
     7321 tcgaatctcg atcatttcac tattttccag aattttcttt atatatgatt caattaaatc
     7381 cgaggtattc tgatgtgcca tcttattcat tccctttgac taatattgac cttaattata
     7441 acgaatttga taatagaaag aaagaagaac gctttttgtt aaataaatcc tttagaatca
     7501 acaaaaaaaa atacggaata tactattccg tatcgacgcg cccaagagga gtcgaacccc
     7561 taaccttttg atccgtagtc aaacactcta tccagttgag ctatgggcgc atattcaatt
     7621 taaaaaaatt ataaatcatt tgaaaacaaa aagcaagata aaaaaaatcg ggaagacagg
     7681 attcgaacct gcgacccctt ggtcccaaac caagtgctct accaagctga gctacttccc
     7741 gtttaaaaaa taaaaaagac
//
LOCUS       DACGLW010000107         7596 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.113, whole genome shotgun sequence.
ACCESSION   DACGLW010000107 DACGLW010000000
VERSION     DACGLW010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7596
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.113"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            complement(134..424)
                     /locus_tag="IXT53_002599"
     CDS             complement(134..424)
                     /locus_tag="IXT53_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906369.1"
                     /translation="MGEKKIKGAGILLVMSKALFLLPCTFMFIVLCYFKRRKKWCWID
                     VLLFLPFFPLIYFLEEIWDDMACDSAFLFSHSLGLMISLTIFAIVLFFLNKK"
     gene            complement(542..802)
                     /locus_tag="IXT53_002600"
     CDS             complement(542..802)
                     /locus_tag="IXT53_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906370.1"
                     /translation="MGVDHLIVLSKLLFWIPFIIMTSAFFYLFKRPNKYLLLSACPMI
                     YFIHKIRGFYYYDSGISLFCYEIGLLASAIFFIFVLEYFRKK"
     gene            complement(939..1352)
                     /locus_tag="IXT53_002601"
     CDS             complement(939..1352)
                     /locus_tag="IXT53_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HAQ4906371.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1808..2293)
                     /locus_tag="IXT53_002602"
     CDS             complement(1808..2293)
                     /locus_tag="IXT53_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906372.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2432..2647)
                     /locus_tag="IXT53_002603"
     CDS             complement(2432..2647)
                     /locus_tag="IXT53_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HAQ4906373.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2871..3671
                     /locus_tag="IXT53_002604"
     CDS             2871..3671
                     /locus_tag="IXT53_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ4906374.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3690..5558
                     /locus_tag="IXT53_002605"
     CDS             3690..5558
                     /locus_tag="IXT53_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HAQ4906375.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLKKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5551..6042
                     /locus_tag="IXT53_002606"
     CDS             5551..6042
                     /locus_tag="IXT53_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HAQ4906376.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6030..6584
                     /gene="srlA"
                     /locus_tag="IXT53_002607"
     CDS             6030..6584
                     /gene="srlA"
                     /locus_tag="IXT53_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174078.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HAQ4906377.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6602..>7596
                     /locus_tag="IXT53_002608"
     CDS             6602..>7596
                     /locus_tag="IXT53_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HAQ4906378.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2707 a         1212 c         1443 g         2234 t
ORIGIN      
        1 ttactatgaa taacataata agaaagtaaa gtggattgat ttgagatctt tttatacttc
       61 aaaaaagata acacccaact ttcaaaaata tgttttggaa gttgggacaa acgagttaat
      121 acattttaat acattatttt ttattcaaaa aaaatagcac aatggcaaag atcgtaagag
      181 agatcataag ccccaatgaa tgactaaaaa gaaaagcaga atcacatgcc atatcatccc
      241 atatttcttc aagaaaataa ataagcggga aaaagggaag gaatagtaga acatcgatcc
      301 agcaccattt ttttcttctt ttgaaataac ataaaacaat aaacatgaac gtgcagggaa
      361 gtaaaaacaa agctttactc ataactaaca aaataccagc tccttttatt tttttctccc
      421 ccacattcaa agagtaacaa tggtatgtac ttaataatag aaaaatggat caacttgtat
      481 gaataacagc tgaaaagatc tgcaccaaat aaaaaacttc cagccaaaac tgactggaag
      541 tttatttttt tctgaaatat tctaacacaa atatgaaaaa gatggccgaa gctaataagc
      601 cgatttcgta gcaaaacaat gagataccag agtcgtaata ataaaatcct ctaatcttat
      661 ggataaaata gatcatcgga caagcagata agagtaagta tttgttaggc cttttgaata
      721 agtaaaagaa agcactggtc attatgataa atggtatcca aaacaaaagt ttacttaaga
      781 caatcaaatg atcaactccc atatttttta ccaagagaga tcataacatc tttatcgtaa
      841 aaacaaaata tttttttgct cattttgaaa atttaaacat actcgttctt atccaaatat
      901 ataaaaatcg cccctcattc atcgagaggc gataagttct aatttaactc aatttttttt
      961 gtttcttttg gctgataagc tgttttaggt aataccaatt tcagcacacc gtttttaaag
     1021 gttccagtga ttttttcttc atcaacatta ggaagataga atgaacgttt gtaagagacc
     1081 gaatgtcttt ctttccgtac atagttgcca tcttctttat cttctgtatg ggactcatga
     1141 ttggcggaga tcgtcaaagt attatctgaa tattcaactg tcgtgtcttc tttgtcacag
     1201 ccaggcatat cagcagttag tttgtattct ttttcatttt cgactaaatc tacttttgga
     1261 taacttgaaa cacctaaaaa atcattgaat gctggactaa acacgtcatt gaaatcaggg
     1321 aacatgtcgc gaatgctagg tacatttgcc atttttgatc atcctttcaa taagtatttt
     1381 ttatttatcc ctatttacgt aataactcta ttttgaaaaa aatgcaagta taatggttag
     1441 ctttgtgaat atttcatcta atacgataca aaggctatct ttctaatttg aaaaagggat
     1501 cattaaaata ataagaagaa aatatgtcaa aaggtaaaaa agtaaattct tctatccaaa
     1561 tatagaaaaa ttgaatctag aaaaccgccc gacaataaat ttcattaact cagttgaccg
     1621 aaaatgaagt agataccaaa aatttatcaa gagagcaagt agagaagtga gtatattcat
     1681 aagagaaata gctgtaacga attattaggt taatgcaaat gcttatttat actccgtata
     1741 actttctcag aaaaaatgat accaataatg acttgctagt actgtaaatc cgtataatat
     1801 catctaatca aaatggtgtt ccatttgcat ctgtcatcgt aatcgatcct tcgggtgaca
     1861 ctcgataaaa accaacgctt ccagatcctc cttgggaacg gatttgtttg ctggaagctg
     1921 tgaacaaata atctcgatca attttttgat aaaagctaat aacaatatcg tcactttgta
     1981 attcggaacg gtgggcaata agaaatagaa gtgctttgtc agagttttct aatggaagag
     2041 aaggtacagt gctttcttgc atttcagatg ttgaagaaat gctaaacgac tgaatacttg
     2101 attggctatg ttgcgaatct acattaccag tagaagtatt agtaatggaa gtagtagaag
     2161 tacttttagt tgaaccaacc tcagttgttg aaatggctgc tgaagattct ttaacagaat
     2221 tctttgtttt atatccacag gcagaaaaaa gtattacagt tcccaacaag cctgcgtaaa
     2281 tccctttatg catgatagct cctcctaaaa tagattatct ttattcagta tatcacttaa
     2341 acaaagaaaa gcagatggtt taaaatgaaa aaaccttccc aataaaggga aggttcatga
     2401 agataataac ttttttaata attatagctt tctaatatct ttcatcaact tttctagtaa
     2461 tcattgggaa aaatttcaag cagtagatcg cacaaatacc tacgacgata taaatgatct
     2521 tcgcaaaaat agttgttgaa ccaccagcaa tcatagctac taaatcaagt tcgaacaagc
     2581 ctaccaacag ccagttcaac cctcccacaa tcaataaagt taatgcaata gcatctaaag
     2641 ttttcattag aattctcctt tcttaaatga taaaacaatt atatatcaaa aagagaaaca
     2701 ttgcttatat aacgctcagg aattttagca atctatgcaa aaagatgaaa attttttttg
     2761 ctcttccatt agtacacatt ttttagctga agctgtaaat ttcatgtctt aatttttttt
     2821 agtgagcttt tatactggat ttgtaagaaa aaagaaggag aggatcacat atgtcggatt
     2881 ggttggatat tgcaggaaaa gtagtaattg taactggagg ttcctcagga attggaagaa
     2941 gtatcgtcga aaatttgttg aagcagaatg cacaagtagc gaattttgat gtaactgaat
     3001 gccgtataca gcatgaaaac ctactatctt taaaagtcga tgtgtcttct aaaacggaca
     3061 tagaagaagg gatatataaa gtaatgaaac atttcaagac gatcgatggt ctagtcaata
     3121 atgcaggaat aaatattcct tctcttctaa tagatagaaa tcatccaaaa agcaagtatg
     3181 aattatcaga acaagttttt gataagatga tcgctgtcaa tcaaaaaagt gtatatttga
     3241 tgagccaagc agttggaaga atcttggtgc aaaagggatc aggagtgatc gtgaatcttt
     3301 cttctgaatc aggattggaa ggatcagaag gacagagctg ttatgctgct acaaaagcag
     3361 cgatgaacag ttttactcgt tcttgggcaa aagagttagg aaagaggaat gttcgtgtgg
     3421 taggtgttgc accagggata ctggaagaaa ccggtttgag aacacaagaa tatgaagaag
     3481 ctctttctta tacgagaggt ataagcgtcg aacagctgag aaacggttac agtcgaacaa
     3541 gtacgatccc tctaggaagg agtggtaagc tgcaagaagt ggcggatcta gtctgctatt
     3601 atctttcaga aagatcaagt tatatcactg gagtcacgac taatatttca ggaggtaaaa
     3661 caagaggata aaggataggg gggacgcacg tgacgctagt aaacagatgg taccaattat
     3721 tgaaattact agtcactcac aagaaagttt caatccagga agtgcaaaaa gaaatcaaaa
     3781 ccagtaatca aaccataaaa aaagatattg gggagttgaa tgaagaaatt gccggtattg
     3841 caaaaatcat ccaaaaaaac aaacattttg aacttgaaat ttatgatttt gatttttttg
     3901 atgagatcat gcaaggaaaa ttgaaaacag ccagtgattt caattcggca tccaaaagaa
     3961 tcgcttattt actaaatagg ctgatcgaaa cagaggattt cttacgtatg gatgatttgt
     4021 ctgaagaagt cggtgtcagc agaggaacag tggtcaagga tctaaagaca ttaaaagaga
     4081 tgatcaatga ttttgatgta aagattacag gcacaccaaa taaaggacta agaataatag
     4141 gagatgagct agaattacga ctacttttat tttattttgt ttctccctac ttttcagaga
     4201 cgaaaacaga acagtttcta gaagaaaaac aagcaatcaa acattttcag aagaagacga
     4261 atgctagtaa acaagaaatc acccttttaa cgaaagttat cggtatttct ttgaatagaa
     4321 tctctagtaa ctatctgttg aagaagccca tcaatcgtta cagtgggtgt tttgaatata
     4381 tccaagaatt caatgaactg atcaatcagt tggaagaaat ctatgaacta actttgagcc
     4441 aatatgaaaa agattttatc agttttcctt tgaacatcat caacacaagt ataacgatga
     4501 taaggaaagc taactctgat ttgcgcttat atttcgaccc catgatgatg cgtattcgac
     4561 gattagatct cattgatctg gatgagtcct tcttatatca agagatgaaa cagcacctct
     4621 ctttattgat aaatcgggta atttttcgtt atcgattgac tgatttattt tatggtgaga
     4681 tagaaaaaaa gtatcctttt tcttaccaaa tcgctagcga atgcatacaa gcattagaag
     4741 ctatcttgca acataagatc tctaaagtag agatcagcta cctaacgttg tatttggagt
     4801 tagcatttcg atatcaaaaa gagcattttt cctctaaaaa agtagccatt gttcatacga
     4861 caggaaaagg aacagcatta atgatccaaa gacaaatcaa aaggatattg gggagggata
     4921 tacaaataac acatgtttca gaagaacaat atgaaaaagt agatctaaac aaatatttcg
     4981 ctgtttttac aacgattccg ttaaaaaatg taaatgaaga gacaccggtg atccatttga
     5041 ctaatctttt tgatgactct tggttaaggg cacaatggca aaaagcaaaa aaaaagcgat
     5101 tagccgggtt tcattctact gtttttttct atcaaatatg gaaagaaaaa aagacgtatc
     5161 aagaatattt aaagaaatta gtcgctcctt tgatcagagg ctattatgtg gatgaagaat
     5221 ttgaaaaaag gatcttgaaa agagaatatc agcaaagcac atttgttaac aaagaatttg
     5281 cttttcctca cacaaccaac cagctatctc aacacattgt tttatcagta gggctaataa
     5341 ctgatcaagg tttagcagaa acaggaggaa atggagttat ttttttattt gccatacctg
     5401 aagaaatgac ggctgaagtt gagaatgaat tatttgagct atacgatatc ctatttgaac
     5461 tcataaaaat gggaatagat gagtttattg aattagcacc gacacaagaa tcatttttaa
     5521 aactaatcaa aagtaaagga gagagacttc atgaatgata tgtatctatt catagttttc
     5581 gcagcttcag cttatgtgct gcaagcagtt atcagttgga agcaactaaa gcatttcaat
     5641 caaacatata gcgaactgcg aagaaaagga aaagtagcga ttggtaaacg acctgggaaa
     5701 atccgttcag gcacgattgt tatgctagct atagacgatc atgatcaagt tttagaagtc
     5761 agaaaaatgc aaggggttac atctttggca aaatttcatt cattacctgt ctatgcgggt
     5821 gaagatatcc attacatcga tcgttaccat ccctcagttc gagcggaaga tcgtttgaca
     5881 caagcagctt tagaaaatgc cagagaagta tacttacgaa tagaggcagg aaatgaaaaa
     5941 agtgaacaac cttcattgcc ttttattgct atgctcaata attttcggat atggaaaagt
     6001 acgatcaaag aaaaaaggag tgacaaacga tggatatcat aacaaaattt gctgagggat
     6061 ttatgaggct gttccaaaca ggtgcagaaa cattcataag ctggatgaca ggaatcgtac
     6121 cagttgtctt gatgctgatg gttgcaatga atacgctgat tgctttttta ggtgaagacc
     6181 gagtaacgaa agtagccaaa ttatcagcga aaaatccgct tactcgttac cttgtgttac
     6241 cgttcttatc tgcttttatg ttgggaaatc ctatgtcatt tacaatggct cgtttccttc
     6301 cagaatacta caaaccaagc tattatgctg ctcaagctca attttgtcat acgagcaatg
     6361 gtgtattccc ccatattaat cctggagaat tgtttgtatg gatggggatt gctcaaggga
     6421 tcgaaacctt agggctaaat tcgatggagc tggctatccg atacttgctt gtgggactgc
     6481 tgatgaactt tattggtggt tggataactg attttactac tggatttgta tgcagacagc
     6541 aaggaatcgt tcttagcaaa aaagtagagc tttcagttga ttagagaaag gagagtttta
     6601 aatgtatggt gatattaaga tcgtaagagg aaacggcggg tatggaggtc ctttgattat
     6661 taggccgaca aaagataaac acaagttcat ctatgtcaca ggtggaggtg agaggccaat
     6721 gatcgtagat aaaatagaag aactgacagg tatggagtct atcaatgggt tcaaaacatc
     6781 aatacctgat agtgaaatcg cgcttgcaat cgttgactgt ggagggactc ttagatgtgg
     6841 aatttatcct aaaaaaggga taccaaccat caatatcgtt tccacaggaa aaagtggccc
     6901 attagcacaa tatatcactg aagatattta tgtttcagca gtagggttag aacaaataca
     6961 gccactaatg gaaactgatg ataacaaaga aacagtaaat actgcatctc cagataaaaa
     7021 gtacaaatat gatacttcta aaaagataac cgagcaaaaa gcagagcagc aaaactggat
     7081 agcaaaaatt ggaatgggcg cgggaaaagt agttgctaca ttcaatcaat cagcaagaga
     7141 agcagtagat acgatgatcc ggacagtgat cccttttatg gctttcgttt cccttttgat
     7201 tggtgtgata caaggttctg ggatcggtaa tgtatttgct aacattatgg cacctttagc
     7261 aggaaatgca gttggattaa caatcattgg ctttatttgt tcactgccat ttttatctcc
     7321 tttgcttggt ccaggggcag ttattagcca agtgattgga acattgatcg gtgtagagat
     7381 tgggaaagga aatattcctc cccaattagc attaccggca ctatttgcta tcaatactca
     7441 aaatgcatgt gattttattc cagtcggtct aggtttggaa gaagcaaaag ctgagacagt
     7501 agaagtaggt gtaccatcag ttctttattc aagatttctt aacggcatcc cacgagttat
     7561 cattgcttgg gtagcaagtt ttggattata tagctg
//
LOCUS       DACGLW010000108         7350 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.114, whole genome shotgun sequence.
ACCESSION   DACGLW010000108 DACGLW010000000
VERSION     DACGLW010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7350
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.114"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            complement(<1..2269)
                     /gene="esp"
                     /locus_tag="IXT53_002609"
     CDS             complement(<1..2269)
                     /gene="esp"
                     /locus_tag="IXT53_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HAQ4906379.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLVGVGLVLGTT
                     ELVQAQDEISPSTPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEEPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSASAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGKKVADLAIERRKDHENYGVGSITDNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSILDVYNKDFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDI"
     gene            complement(2886..3191)
                     /locus_tag="IXT53_002610"
     CDS             complement(2886..3191)
                     /locus_tag="IXT53_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906380.1"
                     /translation="MLNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDA
                     KISSYLFFLIHDEFMDYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            complement(3336..4541)
                     /locus_tag="IXT53_002611"
     CDS             complement(3336..4541)
                     /locus_tag="IXT53_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HAQ4906381.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKDFFKLKMNNL
                     NVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTGNLSKT
                     IEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDT
                     IRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIV
                     YYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMH
                     ITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLG
                     LVDNFNNIS"
     gene            5235..5621
                     /locus_tag="IXT53_002612"
     CDS             5235..5621
                     /locus_tag="IXT53_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HAQ4906382.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFNKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            5719..5910
                     /locus_tag="IXT53_002613"
     CDS             5719..5910
                     /locus_tag="IXT53_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HAQ4906383.1"
                     /translation="MNTLKEDFILAKNGNEEAVVAILKRFSALVHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            6424..6756
                     /locus_tag="IXT53_002614"
     CDS             6424..6756
                     /locus_tag="IXT53_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HAQ4906384.1"
                     /translation="MKKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            6666..6869
                     /locus_tag="IXT53_002615"
     CDS             6666..6869
                     /locus_tag="IXT53_002615"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ4906385.1"
                     /translation="MFYEHKDRYGSPGITQKLYDEGIEINKRVVAVLMREMNLCTRGF
                     HRRKSSHGREKTIKEMVKKMLNR"
     gene            7053..7208
                     /locus_tag="IXT53_002616"
     CDS             7053..7208
                     /locus_tag="IXT53_002616"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HAQ4906386.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLTDHHITHSMSQTGTPVDNAVIE
                     AFHRSIK"
BASE COUNT         2462 a         1299 c         1046 g         2543 t
ORIGIN      
        1 aaatatcttt acttacagtt actgctaaat cggtcgtgcc tacagaacca tcttggtaaa
       61 caattttaat aggaattgtt ccagtaatcg gctccgattc tcctacacct ttagctggag
      121 tgaatgtaac aataccagta tttgcatcaa cacttgcatt accgaagtcc ttattataga
      181 catctaaaat tgaaaaggca tctttagcta aaggaacatc tggtttatca aatgtaggtt
      241 ttccactttg aactgtcgtt tctggagttg ttgttatagg tacgtatgtt gcatcatttt
      301 ccatagcttg agaagctttt acagcaaact ctacatccac gtcgaaagtt cgatttccga
      361 tcttaacagt tacaatacct ttcgtactat cagtaacgtt agaagtatca ggctctgttt
      421 tccatgaata ctctgcatct tcaggtaagt ttttattatt ccgaattgat tctttagcat
      481 ctggtttaaa tccaactacc acggtttgtt tatcaaccgc ttttggtgat tccttaataa
      541 cggttgaacc ttcttctggt ttatcaaaac ctggagaaac gatttggatt tttaattcat
      601 ctttcgcgat taatttactt gaatctacac ccgtaaattc aaattctaca gtatctgaaa
      661 ttggagcccc atctttttca tttggagcga tagttttttc tgctaataca gtaccttctt
      721 tatcaacaag ttgggctttg tgacctgttc ccgctaactc gtggatgaat actttcccct
      781 taactgttgt gtcaacatca tatacttctt ctaattcagg tttcccgata tatggattcg
      841 tgtctccgct ctcttcttta tcgtcagggt ttccatcacc atcaatatct tgcaaataat
      901 aataacctgt tccataagtg ttctggatta attttttatt cgtatccgac aaccaagaat
      961 caaagatgaa atcttcacca aatggtttat tttcttcaac ttgtgctgtt gctttcatat
     1021 cttctaagat ttcatctaat ggcttagtca attgtaaaac atatcgaata acgactggac
     1081 gtggacttgc attagcaaaa tcaataagtt tggtaagatc attatcagta attgaaccta
     1141 caccataatt ttcatgatct ttgcgccgtt caattgctaa atcagccact tttttccctg
     1201 attcaaaaga aacatcatat gttactttgt cagcctgaac catgtgcatc tgtatacccg
     1261 ctacatatgg aactaatcgt tcatcaattt tgtaattagc ataccaaggc gtgccctttg
     1321 cggaagctga atatgagaaa ttataatttt tcgttaactt atgatctaag attatagctc
     1381 cattagcacc tgtggatcct gaaccatctt ttgtttgagt attaggttcc tcatattttg
     1441 cacttactga tccatgttta aactggtcag gattattttc agatacatct aagctattat
     1501 agaaatcatc tgaatctgtt aaaaagtagc ccgtactatc aatacctggt acaattttat
     1561 catcgtcttg atgacttacc aagtagattc gatacattaa acgatcggtc ttgagattgt
     1621 ctatgcctat ctctttaaaa atttcatcta agctcttttc aaacgtaatt accccattag
     1681 gagcagtttg tgtatcagtt gtttcagccc cagcaaataa tccatcactt gcacgaatat
     1741 aattgacctt ccaaatattt gtaggtctac ccagcgaatc attaattctt acaaaggtcc
     1801 gtctagaaga tccaattcct ttctttggat cgacctgaat tttttctaca tatttagcta
     1861 atcgttcatc taattgaaga cggatttccc agtttttagt tgatgctgca atattatgtt
     1921 ttccgtaaac ttcaaatcca attgtattgc cttttagtgt gtctccagtt agttcttgag
     1981 gactaaattg taaatccgaa aagttataat ttttcgtata accaggattc tcctgaaaag
     2041 tattccccga agctactttg tcacctactt gtacggagct tatcgctgtt tcaagtgggg
     2101 tgcttggaga aatttcatct tgtgcttgaa ctagttcagt tgttcccaag acaagaccaa
     2161 caccaaccaa aacagatgca accccaacac ttaatttttt gatactgtac ttcaatactc
     2221 gtttctttgt tttttctaga aatactctct tattattctt gctaaccatt attcaaatca
     2281 actcccttaa tagttatttt cttagtaaga ataaaagata aatctgatac gtattattca
     2341 cagaatagga atcttactaa acttatgtta actagtttac ttaaagaatt tcatgcacat
     2401 tacaacaaaa aaagtacatt tcttcatgtt tttttcggat atttgtgctt tttaaccatt
     2461 cagcagctga aaacatataa aaagatatgt atgtattctt tctgaaatta agttcatttc
     2521 tatgcgatga agcatgtttt caaaatatta ctctttctta gattaaactt cagagtattc
     2581 aaatagcgga ataaggagcc ttatcccgca ttactgaatg agactgtgac ataagtaaaa
     2641 aggtgacttc aaattccttc ttcggaattt gaaatcacct tctttataat tataaagtat
     2701 cggtttcaca gcattcctga atgattccta gaatcttaaa cgaatgattg gagttatcaa
     2761 cattttttca ctcgttttta gcgaaacaaa aaatgacatt tgtcacagcc tcattaaatt
     2821 attgattctt tttctttata attgaattgt ccatatttaa agtgtctagt actctacatg
     2881 aataattaat tgctttcact caaatactta taatctatta acttgtagtt gtttaacttt
     2941 tctgaaaaag ttagacccac gtaataatct tcccagcttt ctctttcaat taactgatcc
     3001 aatttttgat aatccataaa ttcatcatgt attaaaaaga ataaataact tgatatttta
     3061 gcatctagct taatcagatt aaataatgtt tgcttaaaat tgctaccatt aatatgactg
     3121 atagattctc tagcttcttc acataaactt tttactttat taattactac tttgtcatat
     3181 tcatttaaca cactattatt accattactt agacgctcag attctacgaa acaactcaaa
     3241 tcagaaattt cccaaaaaat tttttgttaa aatccaattt cctcaactcc tccaaactac
     3301 gtaaatgata tagtctcata ataaaataaa acctctcagc taatgttgtt gaaattatcc
     3361 actaaaccta attgatattg cttaggagat acacctgcgt actttttgaa tactctcgaa
     3421 aagtgtgaag aatctgaaaa tcctaaacct aaagaaactt cactgatagt atggtttgat
     3481 ttcaacatta ttttagccgt agaaattttt cgcttagaga tataattata aatcgtaata
     3541 tgcatttcat tcttaaagct ttctcttaat ttagtttcac ttacagaaaa ataattagct
     3601 atttccttta cagtaattct tttatagata tttaagccga tgaattgaac gacagaagaa
     3661 attaagggag aaagatgctc tatcttagtt tttccgattt ccttagtata ataaacaatc
     3721 ccagaatccc tcacttttaa gacctcaggt aacgatacag ctaattcgtt ttcttgtatc
     3781 aaagcatctc tactttgata cgcttctatt atatccattc ctaatgttat agacgtttgt
     3841 gctaactttt caaaaacaat gatagaataa tttttttcag accgaatcgt atctccacta
     3901 acacttggaa ttattccatt actcagatta aaaaccatgt tttctaaatt tcgaatatct
     3961 ccatttttaa catattccag tattttattt tcataaagaa acagatatat ttctggatcg
     4021 taattttgtg ataacagatt atctattttg atttgctcga tagttttact tagatttcct
     4081 gtatattcaa ttaacggttc atggaataga tcttctattt ttccagtaaa aaaataatga
     4141 actaaaatca gaatatctcg aatgtctcct aaagaataca aagtcaaatt ttgcaatgaa
     4201 ttatagagtc gttctctatc agccattgca acattcaaat tgttcatttt tagtttaaaa
     4261 aaatctttat caatattatt tgctctaaat ggaccaaata agtaatatct ggttttaaac
     4321 atgtatagaa gaaacatctc attaaaatca ccagtaaaga aatggaaatc tttagttttt
     4381 ttaatattat cagttagtaa ctttttataa tcataaaaaa aataagtagt tctatctgag
     4441 cgatactctt ttataatact gaattcggaa ttaaaaacca cgataggtaa ctttaaacaa
     4501 acatgaagag actttaataa gacttcttta tctgaaaaca cttttgtttc tctctttcct
     4561 ttctacatca ttaaatagta ttttatacgt tatctaaaat actattcctc tgtcaaaata
     4621 agctaactta cttcattata aaatatccac ttatcgcttt catgtatatt ccaccgtaaa
     4681 aagtacattt tatcaccgag aactcatcat ttttcatcat gatgaatact atttacatat
     4741 atctagttca aagacaataa ttattatgat ccgttattat ctaagcacta tacagataat
     4801 aggaattttt cgtttctaaa tactccaatt tgggaatcca ataaaaattt tcgttcaaat
     4861 acgtgtctat tcgaactctt ttctagttgt taattatgaa gggggaagaa ataaaaaata
     4921 caatctatac acgaataaga atattatcca aaaggaggtt ttagaaaaga tttattatac
     4981 gtaaaaagct caagacattt tcttagccat aaggacatgt ttgatattta agaagatata
     5041 tgaattttct tttctaagta taaagataac tatccattat caactaaaaa aacatttttg
     5101 aaaataaaca tcagcagtcc tatcaatgca tgtgagctta tagaagttat tcgcagcgca
     5161 gcataattgc attagcttct tttcgtgttt tttgcctctt ttccctttaa atcttaatgg
     5221 aggaggtgtg caatatgtca gataacgacg aacgcgtaaa tgcccagttg attcgattct
     5281 ttgaaaaggt cattttgaac accgctaata cttattataa aaaacaagaa gaaattcaaa
     5341 aacatgaaca acttgatcaa ctcccaccat atttgtttac taaagatact ataaaattta
     5401 aacaaccgat taatgtttta aatgtgactc tccttgttga agatataaaa ttagcaaaaa
     5461 tccttccttt gttgaaagaa cctgaacaaa tttttcttat cgaaaaattt atctttaata
     5521 aaactgataa agaaattgga aaggttctgg gtattacaag acagggtgcg acaaatttaa
     5581 aacatcgact ttacaaaaaa attcaaaaaa tgctggttta atatctgcat aataactaat
     5641 tttgtatgct tctttgctcg tcctcctcct tccccaaaaa ataaatagat tataagttca
     5701 tgggaagaag gtaaatttat gaatacttta aaagaagatt tcattttagc aaagaatgga
     5761 aatgaagaag cagttgtggc cattctcaaa agatttagcg ctttggtcca taagcaatcc
     5821 tggagaactg gaaaatatga tcaagattgt tatcaagaat gtatgcttgc aatatatcta
     5881 gctatctcga aattcgaaat taaagaataa ctcaactaag ggagtctgtg acataagtaa
     5941 aaagatagtt tcaaatttcg aacaataaat ttgaagccat cttctttata attataaagt
     6001 atcgatttta cagcgttcta taatgattca tagaatctaa aacgaatgat tttaattatt
     6061 aacgtttttt aattcgtttt tagcttaaaa ataacttttg tcacagtctc actcctaaaa
     6121 ttactttatt tacgtacgaa gtgggcagga ggtcgaatcc agaactgggc catggtactt
     6181 caacgaggac ttctcctcta gaattgaaaa gcatgcgatt taccttccgt aacgacggta
     6241 gatttattca actatgtgtc caatcaaaat ttgtctgatt tcaaaaatag tcaagagaat
     6301 tttggttgtt aaaaactctt atctatcaac ttccacagtt tagttgacaa accctgatac
     6361 taagttgggc tatgatacac ttttcttgac tatcagttac gttaccggaa ggaatgaact
     6421 ctattgaaaa aaacatatga tcgtaaattt aaactaaaga tttcacagga tatccttgag
     6481 aaaaaagtaa ctaccaagaa aattgctgaa gaatacaata tctctcgtcc aacaatttcc
     6541 cgatgggttt cagaatatcg tcgatacggg aaaaatgctt ttgctggaca aggaaaaaga
     6601 ttacctgata aagcagattt ctacattctt gaacaggaaa ataaacgact aaaagaggaa
     6661 gacgagtgtt ctacgaacac aaagatcgat acggatctcc agggatcact caaaaacttt
     6721 acgatgaagg aatcgaaatc aataaacgtg ttgtagctgt tcttatgcgt gaaatgaatt
     6781 tatgcacaag aggatttcat cgacgcaaat cttctcatgg tagagaaaaa acaatcaaag
     6841 aaatggtaaa aaaaatgttg aatcgataat ttgaacagaa ccaaattgat gctgtttggg
     6901 tgacggatat tacttacatt ccatgtaatg acggtcgcct ctatttgtcc acctatattg
     6961 acttagcaac tcgtattcta cgttactata tggtggattc acacatgaaa aaagagattg
     7021 tcgtccgtcc attacaaatt tattaaggag aattgcctac ggtaattcat tccgatagag
     7081 gtagccagca tcggtctttt atttatcaag agctgttgac cgaccaccac atcacacatt
     7141 caatgagcca aactgggact cctgttgaca atgcagtaat tgaagcgttt catcgatcaa
     7201 tcaaatgaga attaatcgac tccaacaaat ataaaagtaa aatagagatg aaggtgttaa
     7261 tccaagatta tttagaagac tactacccaa acaaaagaat ccatacaaaa tttatgatga
     7321 ctccttgaca atttgaagcg aagaaaataa
//
LOCUS       DACGLW010000109         7302 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.115, whole genome shotgun sequence.
ACCESSION   DACGLW010000109 DACGLW010000000
VERSION     DACGLW010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7302
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.115"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            complement(35..2146)
                     /locus_tag="IXT53_002617"
     CDS             complement(35..2146)
                     /locus_tag="IXT53_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ4906387.1"
                     /translation="MEGIMENKIHCTTGRVITLVLFLFLLLFARGTAVQALEKVDPSQ
                     IKENNNLVLYHDSDSLRNIEVTGINGFKILDEYSTKKQKVLAWPKVKDLKSTSKIVAH
                     YQKVGTYLGKEIDCDVTFSNFVAHAGVNQDGLAKTINGKKYYNYLLFDKNIYRGFFMQ
                     SINVMNAKLEFKYAGTKTKVALSEPQPEIQSKMRSMRAAGIDLNDTFLSIYSLNGQYQ
                     QRVSWYNDNNESVGERNIDMREFVGYEKMGSLPYFVTTDTACAEYKAPALSTNNLTSK
                     VVGGRAEKAYSSNDHFTDVVGADTYHRSGVTYTLQGASPTFMIGANTNSMMFSFDTAL
                     LWTPQPSKPTKEVFNKANTEEAAHNIDKKVIPQGSDVYYHIHQKFDALTVNTMNKYKS
                     FKITDTFDSKNFDMVSDGKNYDGALWYVEANGTWKKADTGGKLTVSGNTVTYTASDSF
                     LANAANYGRDYILQLHMRAKNDAKGIAPNVATVTFNNDPNKGTQKTNTVENYFSLKPN
                     KKVEQDGKDVDGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDIASDHYKQFSIQD
                     PVEDRLTYKEGSARVIDMSTGKDITSEGTLTYDSNSRTLKWEASADFLSKNLLDGREI
                     QLIFTAKTPLNEELDIDNQAKVSVESIEKATNVVTIGVKPNLPQIIVPKTGSNHLVLT
                     VIGSILFLTLASLGYVVLKLK"
     gene            complement(2435..3259)
                     /locus_tag="IXT53_002618"
     CDS             complement(2435..3259)
                     /locus_tag="IXT53_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906388.1"
                     /translation="MNKRKWLFVCVAALFSTVAYSQLSFADSSDSSTTQTIDNSITAP
                     SETDSSENTTEETADSNISFTESTNPTSESSSETSEKPQDVEDTQVIKQAIHIQKIIS
                     SNQINNQGSQVAQQEGINGAKFIVYDITNILEEMSQDDNEKNQSVEIIESKLKDRAKK
                     LSYDQLKKVAEGETKTIDDQEGVIEFSIEVPANKKQAYYIVNESSPENISNSEDIVLL
                     TPVSNEKGEFLTDVWLYPKSEKSEPKEEIKKVVSTGVKKNFFENCWEFFAQLFFDN"
     gene            complement(3256..4011)
                     /locus_tag="IXT53_002619"
     CDS             complement(3256..4011)
                     /locus_tag="IXT53_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HAQ4906389.1"
                     /translation="MIIRKPPKRRVMHKLMIFFWLLSAVAVYLIPVVETNVAKFQQQQ
                     RILAIENLEKTDSSLDVAKIELGDPVGVLTIPSISLKLPIYDGTSDKILENGVGITEG
                     TGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGQKFFIKVNEEQHAYQIYRIEEVK
                     AEELQKNYLDYLMPEKDQDRITLMTCTPKGVNSHRFLVYGERVSFSKEDLKLQKKAKN
                     RKSISLDWRLALLFAFFCVPIIFIYKKIRRRSE"
     gene            complement(4024..6000)
                     /locus_tag="IXT53_002620"
     CDS             complement(4024..6000)
                     /locus_tag="IXT53_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HAQ4906390.1"
                     /translation="MAINRYVKKAGMLVGMLGIIAGCFQGYQVHAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPKNIVSKLSFELTK
                     FGDDGADQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GKGLSYVAEKADIKVTNNDGSVVLKENTDYKIENSDSGFVIDFIVNNGQVSDTVANLH
                     GQDLKMTYNMFVNDSAEIANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLTDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGDYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQSYVLISCLLLGAGGLSAVVYFKKKA"
     gene            complement(6053..6724)
                     /locus_tag="IXT53_002621"
     CDS             complement(6053..6724)
                     /locus_tag="IXT53_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HAQ4906391.1"
                     /translation="MKKWLFGFLGVTLIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPGYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            complement(6784..6993)
                     /locus_tag="IXT53_002622"
     CDS             complement(6784..6993)
                     /locus_tag="IXT53_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906392.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVAQYINDMQLT
                     GKEKACLLGSMKNFEQLRRTYRIHF"
     gene            complement(7060..7299)
                     /locus_tag="IXT53_002623"
     CDS             complement(7060..7299)
                     /locus_tag="IXT53_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906393.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKEVFKALSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
BASE COUNT         2011 a         1496 c         1101 g         2694 t
ORIGIN      
        1 aaaacaaggc tgattctcag ccttgttttt atatttattt gagttttaaa actacatagc
       61 ccaatgatgc taaagtaaga aataatatac ttccaataac agttagcact aaatgattcg
      121 atccagtttt tggcacaatg atctgaggta agttaggttt taccccaatc gttactacat
      181 tagttgcttt ctcaatactt tctactgaaa cttttgcttg gttgtcgata tccaattctt
      241 cattcaaagg agttttagct gtaaatatca gctgaatttc tcgtccatct aaaagatttt
      301 tacttaagaa atcagcggaa gcttcccact tcagcgttct tgaattgcta tcataggtta
      361 gtgtaccttc tgaagtaata tcttttcctg tactcatatc tatcactcgc gcagaacctt
      421 ctttatacgt caaacgatct tccactggat cttgaatact aaattgcttg taatgatctg
      481 aggcaatgtc tacacctttt gtatgccatt tttgtgtgac tagatattgt acttcagatc
      541 ctgcatttaa aggagctgta ggcgttcctt ttttaccatc atttcgacca tcaacatctt
      601 tcccatcttg ttccactttt ttatttggtt taagagagaa atagttttct actgtatttg
      661 ttttttgtgt tcctttgttc ggatcattgt taaacgttac agtagccaca tttggtgcaa
      721 ttccttttgc atcatttttt gctctcatat ggagctgcaa aatataatcc ctaccgtaat
      781 ttgcagcgtt agctaaaaag ctgtcagaag cagtataagt gactgtatta cctgatacag
      841 ttaactttcc tcctgtatca gcttttttcc aagtaccatt cgcttcaaca taccacaatg
      901 cgccatcata gtttttccca tccgatacca tatcaaaatt tttgctgtca aaggtatcag
      961 tgattttaaa tgatttgtat ttgttcattg tgttgactgt taatgcatca aacttttggt
     1021 gaatatgata gtaaacatct gatccttgtg gaatcacttt tttgtcaata ttgtgtgctg
     1081 cctcttcagt attagctttg ttaaacactt cttttgtagg cttcgatggt tgtggtgtcc
     1141 atagcaatgc agtatcaaag ctaaacatca tactattcgt atttgcgcca atcatgaatg
     1201 ttggggaagc gccttgaagc gtatacgtta caccacttct gtgataagta tcagctccta
     1261 caacatcggt gaaatgatca ttcgagctat aagccttttc tgcacgtcct cccactactt
     1321 ttgaagttaa attgtttgtt gataacgcag gagctttgta ttctgcacat gctgtatctg
     1381 ttgtgacaaa ataaggtaag ctacccattt tttcataccc aacaaattct ctcatatcaa
     1441 tattacgttc accgacagat tcattattgt cattatacca agacacacgt tgctgatact
     1501 gtccatttaa actgtaaatg gatagaaatg tatcgtttaa atcaatacca gctgctctca
     1561 tcgatctcat ttttgattga atttctggct gtggttcact caaagctact tttgtttttg
     1621 ttcctgcata cttaaattct agttttgcat tcatgacatt gattgactgc atgaaaaatc
     1681 ctcgataaat atttttatca aagagcaaat aattataata ctttttgcca ttaattgttt
     1741 tagctaatcc gtcttggtta accccagcgt gcgctacaaa attagaaaaa gtcacgtcac
     1801 aatcaatttc ttttcctaga taagtaccta ctttttgata atgggcaacg atttttgacg
     1861 tacttttcaa atctttgact tttggccaag caagtacttt ttgtttttta gttgaatact
     1921 catctagtat cttaaatcca ttgatcccag ttacttcgat atttctcaat gaatcactat
     1981 catgataaag caccagatta ttattttctt tgatttgact cggatcaact ttttctaagg
     2041 cctggactgc agttcccctc gcaaaaagaa gaaggaacaa aaacaacact aaagtaatta
     2101 ctcttcccgt agtgcaatga attttatttt ccataatgcc ctccatctaa tattttttta
     2161 aacgatcgta agcttttcat tctttttttc ctaataccca cgttaccaat cctgtaagaa
     2221 gaaccaaact aaccataaaa attggattga tcaagagctt tttaatcacc tctaatggca
     2281 tggaagcaca ttgaatgttt agaaaatgaa tgaacaatcg ttgatatctt ggataaattg
     2341 ataattgaga aaaaattaaa ccgcaacaca atattgttgc ggctaaaaag gatagattaa
     2401 aaactaatat tcttattatt ttcatcattt catcctaatt atcaaaaaat aattgagcaa
     2461 aaaattccca acaattttca aaaaagttct tctttacacc agttgacaca acttttttaa
     2521 tttcttcttt cggctctgat ttctctgatt tagggtataa ccaaacatcc gtcaaaaatt
     2581 cacctttttc atttgaaaca ggtgtcaata aaacaatatc ttcactgttt gaaatgtttt
     2641 cagggcttga ttcattaacg atatagtatg cttgtttttt gtttgcaggt acttcaatgg
     2701 aaaattcaat cacgccttcc tgatcatcaa tcgttttggt ttctccttca gccacttttt
     2761 tcaactggtc gtatgaaagt ttttttgctc gatctttcaa ttttgactca attatttcta
     2821 cagattgatt tttctcatta tcatcttgtg acatttcttc taagatattc gtgatatcgt
     2881 agacaataaa cttcgcgcca ttgatacctt cttgctgggc tacttgactg ccttgattgt
     2941 tgatttgatt cgaagaaata attttttgaa tatgaatggc ttgcttgatc acttgcgtat
     3001 cttctacgtc ttgcggcttt tctgaagtct ctgaagaact ttctgacgtt ggattcgttg
     3061 attcagtaaa gcttatattt gaatctgccg tttcttctgt agtattctcc gaactgtccg
     3121 tttcgcttgg tgcagtaatt gaattatcta ttgtttgagt ggtactacta tcactgctgt
     3181 cagcgaaaga cagttgcgaa taagctacag tagaaaagag agctgctaca caaacaaaca
     3241 gccattttct cttattcatt ccgatctcct ccttattttt ttataaataa aaataatagg
     3301 aacgcaaaaa aaagcgaata gcagtgctaa tcgccaatcc aatgaaattg atttcctatt
     3361 ttttgctttt ttctgtagtt ttaaatcttc tttactgaag gaaactcgtt caccatacac
     3421 taaaaagcgg tgtgagttga caccttttgg tgtgcatgtc attaacgtga tcctatcttg
     3481 gtctttttca ggcataagat aatctaaata atttttttgt aattcttctg ctttaacttc
     3541 ttcaattcga taaatttgat aggcatgttg ttcttcattt accttaataa agaatttttg
     3601 cccttttttt actgaaggca aatcatcgaa aagattatct ttataaagtc cactgtgccc
     3661 cgcgattaaa ggatttttac cgtttcctcc tgtaatatcg ccagttcctt ccgtaatccc
     3721 tacaccattc tctaaaattt tgtcggaagt tccgtcataa attggtagtt ttaaagaaat
     3781 acttggaatt gtaagaacac caacaggatc gcctaactct attttcgcaa catccaaaga
     3841 agaatctgtt ttttcaagat tttcaatggc taaaattctt tgctgttgtt gaaatttcgc
     3901 tacatttgtt tccacaactg gaattaaata gactgctact gccgacagca accaaaaaaa
     3961 tatcatcagt ttgtgcatga ctcttctttt tgggggctta cgaataatca ttagctgatc
     4021 cttctaggct ttcttcttaa agtaaacaac tgcagaaagt ccccctgctc ctagcaacaa
     4081 gcaagagatc aatacgtaag attggaaacc gttgctacca gtcattggta cggaagcttt
     4141 ttgattattt ttgatcgtga ttcggttttc ttctttgtat gaatctttgt ctactttgaa
     4201 agagatctct tttgttaatt tttgatagtt ttcaggagct tttgtttctt ccagtgagta
     4261 atctccttca gttaaaccag tgatttcaaa atgaccctct ttatctgatt ttaggattgc
     4321 tgcatttgct acatcgtcta cccaaactgc ttctttgaca ccatctttat ctgtatcagt
     4381 aagtccaccg tagtacttac cttctgcatt cttagctgca aattctgcgc cttcaagcgg
     4441 tgttgctttg atacctgttc caaataggcc gctatcgact tttaagaatt ttgcgccata
     4501 agtcacaacg tcagctttcg ttttttcgtg gtgttcttcc tgattaaatg gattattgtt
     4561 ataaacgaaa tccacgctat ttgttaatgg atttgcgatt tcagcactat cattcacaaa
     4621 catgttataa gtcattttta gatcttgacc atgtaaattc gcaactgtat cacttacttg
     4681 tccattattt acgataaaat cgatcacaaa accactatca gaattttcga ttttatagtc
     4741 agtattttct tttaatacga ctgaaccatc attatttgtt actttaatat ctgctttttc
     4801 agcaacataa gaaagtcctt tacctgctgt atctccccat ttaaatacac tgtaaggaga
     4861 agtttcgctt ttcacaccgt ttactgtgat accgtctgcg ccacctgcaa tgtctgttgg
     4921 aatatgaatc gttccttgat aatttactgc gtcaccaatt tggaaagaat gttcttgtcc
     4981 tgttccattt gttactgttt tatcgatcac tggtgcttta tagtttacgt atgaagcttt
     5041 taagtcactt gtcacgccat cattcgtaat ttcaaaggtt aatttattgt gcgcatcgtt
     5101 gcgagcgtct gcgcctaata aatattgatc tgctgttggt tcttgatctg atcccactac
     5161 aacttccgaa ggaacttcca caaaatagta ttttcctaac gtcaaatcag tcgcagtcag
     5221 ctttccttga tcatccgata caagatcacc caattttgtt ccttttccag gttcgccctt
     5281 gtataactca aattttgccc cttttaaaag ggtttgtttg ctttggtctg cgccatcgtc
     5341 accaaatttc gttaattcaa aactcaactt agaaacaatg ttcttaggat acaaatgaat
     5401 attttttaaa aatcctgatc ctgttttatc tgtcattggc gcaatcacaa ccattggttt
     5461 tgctttttgt gtgactaatc cagcagcgga tttactttca taaaccacat atacattccc
     5521 tttggaattg acataggctg gttgatcagt aaaagtgatc tcaccatttt catctacagc
     5581 tgaagtaatt ttgttggttg cattcttgac gtactcactg ttagatccct tgtcttctac
     5641 atcttgtaca atctcgtcca ctttcgcgtt ggttagatca gaatcttctc tcggtaatac
     5701 ttgatccgta atatttccta acgtaaactc aacatcacca taatctgcct tgttgtattt
     5761 cgttacacct gtaggatatt gatcgtgcgt ataaccatcg tttttgatcc cgtcaatatc
     5821 aacattcaat tgcgttcctt gatcgtacat taatttatga accgtgatat tgaccttctc
     5881 tggtggtgtt ttttgcgtga catcttctgc atgtacttga tacccctgaa agcagcctgc
     5941 aatgattcct aacattccaa caagcattcc agcttttttt acataccgat ttattgccat
     6001 tttttactct cctcatttca tatttcaacg actcgcgttg attaaaacct tgttattgta
     6061 gctctccctg taccacgtat ctttcttttc cttgtgacgt ataaacacaa gtaaacaacg
     6121 ttaataaggg tttctctcct ttctttggca aagacaagaa agagttctct tcaggaacgc
     6181 catcgattaa tttaaagtac ccaggtacga taaatttacg cgttaccttg taacgataga
     6241 cgccatcgtt actaactaag tccatcacct cccctaatga caacttttga atatcagaaa
     6301 ataaaacacc tggcatttcc atgttatgac ctgctaggac atagtttcct ttgcccattt
     6361 cggcatcttc ataaaaataa gtagccgctc caagactcaa agtgtattga tttgcccctt
     6421 taaaaattgg caatgctaaa ccaagtgcag gaatctgaat ttgtgcaaat acttgtttat
     6481 tgacgcccat ttcgattgct tctttttgta tggacaacat ttgccccaaa gacatatcat
     6541 tattgacgtt cgttgcatcc tccttataac tctttgcatg tgcattgatt tgatctacgg
     6601 gcatcttttt ttctatattt tgcttaaaca cctctccttc atgtttttgg tagctaacat
     6661 agccaaacac actgcaaaag acgattaaag tgactcctaa aaaaccaaat aaccattttt
     6721 tcatccatct gcctcctttc agacaaaaca aaaaaacttt ttctcatttc catagaaaaa
     6781 gttttaaaaa tgaattctat aagttcgtct caactgttca aagtttttca ttgaacctaa
     6841 caaacaagct ttctcttttc ctgttaactg catatcattg atatattgcg cgacagataa
     6901 tcttttaagg tgtttttgct tcatcgtttt tagtaacttt tcttccattt ctttcgacac
     6961 tttttttctg ttttcgattt cttcataatt catgagtttt cctaccttcc ttgttttttg
     7021 caaaaataaa aaagcctatg atcacatacg ctctacactt cagtcttggg ttatcttttt
     7081 ctttcgttta attactggcc gtgctggttt caaatccttg attccattat gggttctcca
     7141 ataatcgaat tcttttcgca atctttcttt ttccatatct gttcctttag ataaggcttt
     7201 gaatacttct ttaggtgaat aaccacgagc taacagctgt acataccttc cttttgtcaa
     7261 agtatccata tccatcgcct ctaaaactgg tcgcgccatc ta
//
LOCUS       DACGLW010000110         7265 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.116, whole genome shotgun sequence.
ACCESSION   DACGLW010000110 DACGLW010000000
VERSION     DACGLW010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7265
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.116"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            114..761
                     /locus_tag="IXT53_002624"
     CDS             114..761
                     /locus_tag="IXT53_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HAQ4906394.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     gene            1154..3799
                     /locus_tag="IXT53_002625"
     CDS             1154..3799
                     /locus_tag="IXT53_002625"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HAQ4906395.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4109..5422
                     /locus_tag="IXT53_002626"
     CDS             4109..5422
                     /locus_tag="IXT53_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HAQ4906396.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5412..6065
                     /locus_tag="IXT53_002627"
     CDS             5412..6065
                     /locus_tag="IXT53_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ4906397.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6120..6812
                     /locus_tag="IXT53_002628"
     CDS             6120..6812
                     /locus_tag="IXT53_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HAQ4906398.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            6962..7162
                     /locus_tag="IXT53_002629"
     CDS             6962..7162
                     /locus_tag="IXT53_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ4906399.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
BASE COUNT         2398 a         1232 c         1595 g         2040 t
ORIGIN      
        1 tttgaacacc gttttatttg tctcacaccc ttttccttcc tacttgtaac cttgctcccg
       61 tgttagaaac atgttacact aaatatgtga aattattaac aaggagcgtc gttatgcgag
      121 aaaaaacaga tacactgcca aaagcaactg cgaaaagatt acctttatat ttacgctact
      181 tgaagatgct ggatgactca ggtataagta gaatcaaatc aaatgagttc agcgaaatca
      241 cccaaattcc ttctgcaacg atccgcagag acttctcgca attaggcgag ctaggcagaa
      301 gcggatatgg ctatgatgtc ccttttctta tcgatgtatt caataatatc cttaatacta
      361 aagaagaaaa acgaatcgca ttagtaggat atggaaatct tggcaaagca ttgaaacaca
      421 acaattttag aagaaatgaa aatctaaata tcgtgtgcgt ctttgataat gatccagcac
      481 tgatcaatcg agtaattgat ggagaaatga tctatccgat tgatcgtttc gcggagatag
      541 ccaaagcaaa aaatgtgaca gtagcaatct ctacggtacc aagtaaatat tcacaatctg
      601 ctattgatga aattgtcaaa ggaaatgtaa ccgctatact taattttgcg cctgacagag
      661 taactgtccc agcttatgtc aatgttcaat acattgattt gacaaccgag ttgcagacgc
      721 ttatttactt tgatgagaat tactcagaag ttttttctta ggaaaatttg agtaggctta
      781 ttgatcttgc gagaactcgt cgtaattctt tcaaaaattt tttagtacta aatgaagtgg
      841 ttttttcacg accacttgtt gactaaaaaa aaaaaccatt gtagaatgaa gcataacgct
      901 gaacaagagg agtaactggc acattcattc ttagagagag aactatctgc tgaaaggttc
      961 tcaatgacaa ccagtgaagg tagcttggga gtttctgtct tgaatggagt agagatagac
     1021 gagtcgtgat cgttaactca cttgagaggc aataatgatt gattattgca atttaggtgg
     1081 taccgcgtaa atcatacgtc ctagaatacg aaagtatttt aggacgtttt ttagtttaaa
     1141 ggaggaaaac agcatgacgg aagaaaataa cctatcaaca aaatataatc cgcaagaagt
     1201 tgaagctgga cgttatcagc attggttaga acaagatttg tttaaaccga gcggagataa
     1261 aaaagcaaaa ccttattcaa tcgtaatccc gcccccaaat gttactggaa aacttcattt
     1321 agggcatgct tgggatacga ccttgcaaga tatgctgatt cgtcaaaaga gaatgcaagg
     1381 atatgatact ttatggctgc ctggtatgga ccatgcagga atcgcgacac aagcaaaagt
     1441 agaagcgaag cttagagagc aagggatttc tcgttatgat ctaggtcgtg aaaagttcat
     1501 cgatcaagtg tgggaatgga aagaagagta tgcgagccac atccgcgaac agtgggctaa
     1561 aatgggctta tctttagatt acagtaggga acgttttaca ttagatgatg gattatctga
     1621 agcagtacgt aaagtttttg ttaccttata cgaaaaaggt ttgatttatc gtggtgaata
     1681 tatcatcaac tgggatcctc aagcacgaac agctctatct gatatcgaag ttattcataa
     1741 agatatcgaa ggcgcctttt atcatatgag ctatgaacta gcagatggat caggcgtagt
     1801 agaaattgcg acaacccgtc cagaaacgat gttgggagat acggcgattg ctgttcatcc
     1861 agaagacgag cgttatcaag cgctcatcgg caaaaaagtg attctacctt tagtcaataa
     1921 acaaatccca atcattgcag atgaatatgt agatatggaa ttcgggacag gtgtagtaaa
     1981 aatcactcca gcacatgatc caaacgactt tgaggtaggt aatcgtcatg atcttccacg
     2041 cgtaaatgtt atggatgatg atggtacgat gaatgaacta gcaggaaaat atgctggaat
     2101 ggaacgtttt gctgctcgta aagcaatcgt gcaagatctg aaagaaatcg gtcgattgat
     2161 taaaatcgat acgatgatcc acagtgtggg tcattccgag cggacaggtg tcgttgttga
     2221 acctcggctg tctactcaat ggtttgtaaa aatggcgccg ttagcgaaaa aagccattga
     2281 taaccaagaa acagatcaag cagtggaatt cttccctcca agattcaatc aaacgttttt
     2341 acgttggatg gaaaatgtcc atgattgggt aatctcacga caattatggt ggggacatca
     2401 aattcctgca tggtatcaca aagaaacagg tgaaatgtat gtagggatgg aagcaccagc
     2461 ggatagtgaa aactgggtac aagatcctga tgttttagac acttggttta gttcagcttt
     2521 gtggcctttc tcaacaatgg gctggccaga tacggaaagc gaagattata agcgatactt
     2581 ccctactagc actttagtaa ccggttatga catcattttc ttctgggtaa gcagaatgat
     2641 tttccaaagc ttggaattta ctggcgaacg tccatttgag aatgtcttga ttcatggatt
     2701 gattcgtgac gaacaagggc ggaagatgag taaatcatta ggtaatggaa tcgatccgat
     2761 ggaagtaatc gaaaagtatg gagcagatgc tctccgctgg ttcttatcga atggttctgc
     2821 accaggacaa gatgtccgct tcagctatga aaaaatggat gcttcttgga actttatcaa
     2881 caaaatctgg aatgcttcac gtttcgttat catgaatatc gaaggaatga ccgtagaaga
     2941 gatcgattta tctggagaaa aatcagtggc tgaccggtgg atccttactc gtttgaatga
     3001 aacgatcgaa cgagtaactg agctatttga tcgttttgaa tttggtgaag caggtcgaca
     3061 actgtataac tttatctggg atgatttttg tgactggtac atcgaaatga gtaaagaagt
     3121 actttatgga gatgatgctg tttcaaaaca aaccacccgt agcatcttag tccatacact
     3181 tgatcagatc ctacgtttat tgcatccaat catgccattc gttacagaag aaatatggga
     3241 acatattcct catcagggaa attctctagt agtggcagaa tatcctgttg tacgtccaga
     3301 gtttgatgat gaaacagctt ctaaagggat ggaagtattg aaagaattga ttcgagccgt
     3361 tcgaaatatt cgttcagaag tgaatacacc attgtctaaa ccaattacgt tgctaattaa
     3421 aacaaacgat gctaaaatcg atcaattttt agttgaaaat acaagctata tcgaacgctt
     3481 ctgtaatccc gaagagttga cgatatcaag tgagatcact gcgccagatc tagcgatgtc
     3541 agctgttctg acaggtgcag aaatcttctt gccgttagca ggattgatca atattgaaga
     3601 agaaatcaaa cgattagaaa aagaactgga aaagtggaca agcgaagtaa aacgtgtcca
     3661 aggaaaactt tctaatgaac gttttgtttc taacgcgccg gaagaagtag tagaggcaga
     3721 gcgtgcaaaa gaaaaagatt atttagagaa gcaaacggct gtcaaagaac gaatcgaaag
     3781 tttgcgtact attcaataag aatagaaaaa gagagtgtga aatcagctag ctattactaa
     3841 aagctagctg attgaacatc gttacaggaa agagtttgag cctcactctt agcttcgatt
     3901 cataaggaga tgtgacataa attttgtcgc atctcttttt tagtctactc ttattatgta
     3961 ggagaaggag ggaggtggat catgtatcaa gagtcaataa agtagttgat aaaatcgttc
     4021 ctctctctgt ttcttttctc tttgtcatac ttttattttt cttatgtgtt ctatttgtta
     4081 taataaaaga gaaatggggg aattgttgat gacgattgaa gaagcaatag aatggatcca
     4141 tagtcgatta ccatttggtt cacgtcctgg ccttgaccga gtcgaagctt tattagaagt
     4201 ggtagggcat ccagagagaa aagtaaaaac gatccatatc gctggaacaa acggaaaagg
     4261 ttctactgta gcttatttga gaagcctatt agaagaggct gggctgacag taggtacatt
     4321 tacttcacct tatatcgaaa cgttcaatga acggatcgca atcaatggca aaagcattcc
     4381 agacaatgat ttgatccagc ttgtaaaaaa atatcagcca ctgatcaaac aacttgatca
     4441 atttgaagca gtagctggta ttactgaatt cgagacatta acagcaatgg catttgatta
     4501 ttttttgatg aagaaagtgg acgtagcgat tatcgaagtt ggcttaggcg gcttattgga
     4561 ttctaccaat gtagctgtac caatgctgac gggaataaca acgattggac ttgatcatac
     4621 cgaaatactt ggagatacaa ttgaagaaat cgctgcacaa aaagctggaa tcatcaaaca
     4681 aggcgttcca gtagtaacag gaaatattgc cccagaagct tttactgtca tccaacaaac
     4741 agctaaagca aaaaatgcac cgctttatgt atacggggaa aattataaaa taaactatct
     4801 gcatcctgat gagatgtggg gagaagtatt tgaatttttt aatgaaatag gaaagatccc
     4861 tcaattaacc actccgcttt tagggcaaca tcaagctgaa aatgcaggag tagccatcga
     4921 attgtatttt gtttactgtc aattggaaaa gctgcctttc cgagaaaaag atgttttgaa
     4981 gggattgaag aaatctcgat ggccaggaag aatggagcgt ttgagtaacg agccattagt
     5041 tgttttagat ggtgcccata atacgcatgc aatgaaccgg cttgttgaga atttgaaaaa
     5101 agaattcaaa ggatatcaaa tcaacatttt gttttccgcc ttacgaacta aaaatgttag
     5161 agaaatgcta gaacgattgc agcaagtacc aaatgtccat atttatttga cgacctttga
     5221 acatccaaaa gccatcgaat taagtgaagt atcagattta gcaagtgaga gagtatcaat
     5281 cgtttcattg tggcaattcg gattagcaga tatattggaa aagatgacaa atgaagacgt
     5341 attagttgtg accggttcac tttatttcat ttcagaagtt cgccatttat taatacaatt
     5401 aggaggagat catgaagtat aatgcagtga tttttgacat ggatggtttg ctgttcgaca
     5461 cagagttagt gtattacgaa gccacacaag tagtagcaga caagatgggt tttccttatg
     5521 ataaagacct gtatttaaaa ttcttaggtg tttcagatga agaagtatgg gcgaattatc
     5581 atatgatttt ctcggaattt ggcaaaacaa gtgtacaaag attcatagat gattcatacc
     5641 aggaaacgct tgaacgtttt tcgcaaggag cggtacaaat gaagcccggt gtgttggaac
     5701 tcctctcttt tttagaagaa catcagattc ccaaagttgt cgcttccagt aaccaacgag
     5761 cagtcattga acttttattg aagaagaaca atctgtttga acgatttgag acgattgttt
     5821 ctgcagaaaa tgttcaacga gcaaaaccag acccagaaat tttcttgtta gctaatcaga
     5881 agctaggtac agcgaagaaa gaaacattga ttttagaaga ttcacaaaat ggtattttag
     5941 cagcttctgc ggctgagatc ccggtagtca tggtaccaga tctgcttcca ccatcccaag
     6001 aattgatcaa aaaaacagct ggggttgttt cttctttaca tgaaatccct gcattgttaa
     6061 aataaaaatc catctctttt cgtatcttat tttagataag atagaaagga gatgtttttt
     6121 tgggaaaacg attgatcaaa gaagtaccta ctagctcttt accaagggaa cgtatggaaa
     6181 tttatggcgc ggaagcttta tcggatcagg agcttttggc aatcttactg cgtaccggtc
     6241 aacaccctta tagtgtgatg tcgattgctg gaaatctgtt aaaaacgttt ggaggactgg
     6301 cctctttacg acaggctact ttgcatgagt tagaagagat tcgagggata ggacgcgtga
     6361 aagcaatcga aatcaaagcc ttgatcgaat taggtcgacg aattcagacg gatcatgaaa
     6421 gagcggctcc accggttcgt accagctacg aattggccca gcaattgatt ctggaaatga
     6481 aagatcacaa acaagaacat ttggtttgta tttatttgga cacgaagaat caagtgatct
     6541 taaaaaaaac agtgttcatc ggttcgctga atcagtcagt cgctcatcct agagaaattt
     6601 tccattatgc tgtacgttat tgtgcagcaa ggatcgtttt agcgcataat catccttctg
     6661 gtaatgttat tccatcgcag caggatatga attttaccaa aaggatacaa aagtgtggag
     6721 aaatgatggg gatcactgta ctggaccact tgatcatcgg acgcaaacga tattttagtt
     6781 tgagagaaga aggaatgatg gaagagaagt aagttgcttt cttgcaaaaa aaatgaagca
     6841 agtgtacaat tatattgtaa ttttgtttgg gaatagaagt tgaaacatct gttgcacctc
     6901 tatcgtacac gagcaatgtt acaatttaaa ttagtgcttt tggcacgagg aggatattat
     6961 tatggaaaac ggaacagtaa aatggttcaa cgctgaaaaa ggatttggat ttatctcacg
     7021 tgaagatgga agcgacgtat tcgttcactt ctcagctatc caaggtgacg gattcaaaac
     7081 attagaagaa ggtcaagcag tgactttcga cgttgaagaa tcagaccgcg gcccacaagc
     7141 tgcaaacgtt gtaaaagctt aattttatct gtaaatcaaa ttgatggtag acatcaattt
     7201 ttacgagaga acaaagcgag taacaaaaat acctgccaca agagacaatt tctcttgtgg
     7261 caggt
//
LOCUS       DACGLW010000111         7142 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.117, whole genome shotgun sequence.
ACCESSION   DACGLW010000111 DACGLW010000000
VERSION     DACGLW010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7142
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.117"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            <1..300
                     /locus_tag="IXT53_002630"
     CDS             <1..300
                     /locus_tag="IXT53_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906400.1"
                     /translation="NKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQV
                     ILKGKSYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            361..1821
                     /locus_tag="IXT53_002631"
     CDS             361..1821
                     /locus_tag="IXT53_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HAQ4906401.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDSKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFIPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            1832..4036
                     /gene="topB"
                     /locus_tag="IXT53_002632"
     CDS             1832..4036
                     /gene="topB"
                     /locus_tag="IXT53_002632"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ4906402.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQVGADQGKTFKRLWINSLEKEAIYQGFQQLRDAKETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FKKEPYFEGKSEVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGMIRQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAESIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPIDLGWKKLFGK
                     ENDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            4275..4868
                     /locus_tag="IXT53_002633"
     CDS             4275..4868
                     /locus_tag="IXT53_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HAQ4906403.1"
                     /translation="MKDKIQCIFDKHNGNLSLKDARSEGLSPMTLDRLAEKDKIEKVA
                     PGFYTLKDAFVDELYLAQSIYSRGIFSHETALDLFQVGTYIPKKINMMFPKGYNVSKE
                     KLDQYAIQPHYTNKENFDLGVTETESFYGNKILVYDLERTLCDMWNPRYKASVEVKQE
                     ALKEYMTSTSRNTNKLRRYMDILKSPKEMTLYMLPLY"
     gene            4884..5783
                     /locus_tag="IXT53_002634"
     CDS             4884..5783
                     /locus_tag="IXT53_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAQ4906404.1"
                     /translation="MTPTQFKEKTRNFAAEKHLNPQIVQRYYMMEKLLECIVESPYHD
                     DFVLKGGFLIGSKYGLENRSTVDIDTTYRNSKLTKEKLETVFSELTANPTKDGIRFEL
                     KGLTETREADYYPGFQAKFIAYLENARVPFKLDITSGDSIIPAALMYEHKLMFEDKTI
                     HIPAYPTEQILAEKLHATFSFGKDNSRMKDFYDLYMIPKLERIDEKNLYKSVQGTFKE
                     RNNQSVFKHLYDQEMPQLVKDEGLREKWKRYQQENYFAKDISFESTLTSVDSLMQSIV
                     EQEKAERKQNFKRSQQMNQEMER"
     gene            5786..6271
                     /gene="ssb"
                     /locus_tag="IXT53_002635"
     CDS             5786..6271
                     /gene="ssb"
                     /locus_tag="IXT53_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ4906405.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFSLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRMHADQSSSSPSPTTFEQRDTATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            6637..6897
                     /locus_tag="IXT53_002636"
     CDS             6637..6897
                     /locus_tag="IXT53_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906406.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
BASE COUNT         2616 a         1231 c         1473 g         1822 t
ORIGIN      
        1 aataagaata ttgtgcccgt tggttcactt tggatcgaca agaaagacgg gaaagtaatg
       61 gaagaagata cgggtcgaac gttacatttg aaggatgcgg cgtttattga agatccgcag
      121 cataatcaag tgattttaaa aggaaaaagt tatggaaaag agattcaaac ggcgttacct
      181 tcagaagaat ttgcgctagg aaatccattc cgattttcac ttgaaacagt gtcagaagtg
      241 gccaaacgaa aaaatgaaat ctattatgaa caacaattgt cacaaaaaga acgcgtataa
      301 aacaagtaaa aaagactatc tgaaaaacgg atagtctttt ttcttttggg aggtttgaaa
      361 atgaccgaaa aaaaaatgag tcttattgat cgatgtaaac agatcgatat agtggatttt
      421 gctcgaaaca atggaatggc tgtagtcaat aaaggacgag actatcgctt agaagaccat
      481 gattcatttg tttttgatcg aagaaaacaa cgtttttatt ggaacagtca aaatattagt
      541 ggcgacatta ttgaattagc taaacttttt ttcatagaca aagagatcca agactctaag
      601 caacaattta aagcagccac tgattttatt ctaaagaatg aagacaaaac agaacgagtg
      661 gaaaatcttc attttgaaac agaaaagtac aaagatcacc ccgttgatta tcaaccactc
      721 acagaaaaag gaaggaatta tctgaaggaa gaacgaaaac taccagagtg gctgattgat
      781 tatgcagaaa aagaagggtt gattgccgaa ctaaagccaa aatatgaacg gcaaaatttc
      841 ttagttcgag acgatcgttt agatcatgcg gtcgcctttt tatggaaaga tccacagaca
      901 aaagagacag taggggcgag ttatcaagga acgttcattg attatgaacg ttttggtgag
      961 agaggcacat ataagcatat agataaaaat tcgacagcga atcatggctt taacctaaag
     1021 atcggtgacc ctaagcagtt aaaatttttt gagagtagta ttgatttact tagctatgct
     1081 gcactgaatc gtgatcagtt aaacgatacg tggttagtct ctatggaagg gttaaaacac
     1141 catgtgatca gtcattattt tggtgaggca gtatctgaat tgagaaagaa acaagccttt
     1201 cctcaatcca tcgagatttg tgtggataat gaccgtgcag gacatatttt ctacgaaaaa
     1261 gaacaattaa tgggcgcagt cgatccattt acgaatcaga aagtacgttg tgaacgagga
     1321 atcgctaatg attggcaagt gccaaaagaa tacaaaatca tttatgaaga agtcgcaaaa
     1381 gaagaaaagg tcacaccaga agctattatg gccatacaca agacagaaaa caatctgcaa
     1441 ttaacgaacc aactggtttc cgctcacaaa gtaaatgcgt tttttggcca gcagctttct
     1501 gttaacgatt caatagaagc cattaatttg aaagatattt gtagagaagt cgcaaaagaa
     1561 ttaaaagttt gtgaacgcgt ggatggtacg tacgattttg atcgttttta tcaagaaaaa
     1621 ggggatatta atgcgcagat tcttttctct tataaagcag aacaatacta caaaggctat
     1681 aaaaatcatg aacatgaatt tatacctgaa gtaaaaaagg actggaacga tcaattgaag
     1741 catgagatcc agcaacaaga aatcagaaag caaaaacgtg ccatgttgtt tcaacaaggc
     1801 agacaacaag aaagggagtg aaatcgtcat gatgaaaacc gttattttag cagaaaagcc
     1861 gtcacaagcg aaagcttatg ccgatagctt ctctaaagcc actcgaaaag acgggtattt
     1921 tgaaatccaa gatcgattgt ttcctggtga aactgtgatc acgtatggtt ttggtcattt
     1981 agtcgaatta gattcgcccg atatgtacga tgaaaattgg aaacaatggt cattagaaca
     2041 tttgcctatt ttccctacac attatcacta tcatgttcct aaagataaga aaaagcaatt
     2101 caaggtggtg aaacagcaac ttcaatcggc cgacaccatt attattgcga cagatagcga
     2161 tcgtgaaggg gaactgatcg cttggacgat cattcaacaa gtaggcgccg atcaaggaaa
     2221 aacctttaaa agactttgga tcaactcctt agaaaaagaa gccatttatc agggattcca
     2281 gcagttacga gacgcaaaag agacctatcc aaaatttgaa gaagcacaag cacgtcagat
     2341 cgccgattgg ttaattggga tgaacggtag cccactgtat agtttactct tgcagcaaaa
     2401 aggcatacca ggaagctttt ccttaggcag agtccaaacc ccaacgcttt acatgattta
     2461 tcaattgcag gaaaagatta gaaatttcaa gaaagaacct tattttgaag gaaaatccga
     2521 agtaatcgct caaaatggcg catttgatgc gaagcttgat ccgaatgaaa cccaagcgac
     2581 gcaagaagca tttgaagatt atttaaaaga aaagggggtt caattgggaa aacagccagg
     2641 catgattcgc caagttgaaa ccgagaaaaa aagcgcggca agtccacgtc ttttttctct
     2701 atccagcctt cagtctaaaa tgaatcagct catgaaagct agtgccaaag acactttgga
     2761 agccatgcaa ggcttgtatg aagggaaata tctaagctat ccacgaacag acacccctta
     2821 tattacggaa ggggagtatg cgtatttgtt ggatcattta gatgaataca agcatttttt
     2881 aaaggccgaa tcaatcccaa cccctattca tacgccgaat agtcggtatg tgaataacaa
     2941 gaaagtccag gaacattacg cgatcattcc tacaaaaacg gttatgacag cagcggcttt
     3001 tgaacaacta agtccactgc agcaggccat ttatgaacaa gtgttaaaaa caacggtggc
     3061 catgtttgcg gaaaaatata cctatgaaga aacaacgatc ttgacgcaag tccagcagct
     3121 tcagttaaag gcaatcggaa aagtaccgat agatctaggt tggaagaagt tatttggcaa
     3181 agagaacgac ggaaaagaga aggaagaaga gccgctactt cctaaagtga caaaaggaga
     3241 aatggtgacg gtagatctac aagtccttga aaaagaaacc aagccaccac agccttatac
     3301 cgaaggtacg ttgatcacgg cgatgaaaac agctgggaaa accgttgata gtgaagaagc
     3361 gcaatccata ttgaaagaag tggaaggaat cggtacagaa gcgacacggg cgaatattat
     3421 tgaaaccttg aaacaaaagg aatatatcaa ggtagagaaa aataagctcg tggtgacgaa
     3481 taaagggatt ttgctttgcc aagccgtgga aaaagagccg ctgcttacga gtgccgaaat
     3541 gaccgccaaa tgggaaagct acttgttaaa gatcggtgaa cgaaaaggaa cacaaaccac
     3601 ttttctagcc aatatccaaa aatttgtctc tcacttattg gaagtcgtac cagggcaaat
     3661 ccaatccact gattttggat cgacgttaca agaagtgaaa gcagccagtg aaaagcaaga
     3721 agccgcgcgt catttaggaa tttgtccaaa atgccaggaa caagaagtct tactctatca
     3781 aaaggtggca gcatgtacgt ctgaagcctg tgatttcaaa ctatggacaa cgattgccaa
     3841 aaagaaactt acagcgacac agctgaaaga gatcattcaa aatagccgaa caagccaacc
     3901 agtgaaaggg ttaaaaggtc agaagggttc gtttgaagca acgattgttt taaaagaaga
     3961 cttcactaca ggctttgaat tttctgaaaa gaaaaaaacg aacttcaaga aaagaacacg
     4021 acgcactaca aaataatgga gggttaatct tatgcgtaat acaacacaca aacaacaaga
     4081 aatcgaaaat caaaaagcca aaattcgttc agaacaggaa aaaatgcgtg ccagcaaacg
     4141 aagactgcag caattgaaga accagcagcg gattcaaaaa gaaatcgaat tagatcgata
     4201 aattcctttc ttgttaatat aaaactaaca tgttatagtt gtgttagttt tatattaact
     4261 ttaggggaag agtaatgaaa gacaaaatcc aatgtatttt tgataaacat aatggcaacc
     4321 tatccttgaa ggatgctaga agtgaaggat tatcgcctat gacgttagat cgattagccg
     4381 aaaaggataa aatagaaaag gtagcaccag gtttctatac cttaaaggat gcgttcgtgg
     4441 atgaattgta tctcgctcag agtatctatt ccagagggat tttttctcat gagactgcgc
     4501 tcgatctttt tcaagtagga acatatatcc ctaaaaagat caatatgatg tttcctaaag
     4561 gatacaatgt atcaaaggag aaattagatc agtatgccat tcaaccgcat tacactaata
     4621 aagaaaattt tgacctggga gtcacagaaa cagaaagctt ttacggcaat aaaattttag
     4681 tctatgattt agagcgaacg ttatgtgaca tgtggaatcc aagatacaaa gcaagcgtag
     4741 aggtaaaaca agaagcatta aaagaatata tgacaagtac cagtagaaat acaaataagc
     4801 taagaagata catggatatt ttaaaatcac caaaagaaat gactttgtat atgttgccac
     4861 tttattgaga ggagtaaaga tgaatgacac cgacacaatt taaagagaaa acaagaaatt
     4921 tcgcagcaga gaaacattta aacccacaaa ttgtccaaag atattatatg atggaaaaat
     4981 tattagaatg tattgttgaa tcgccttatc atgatgattt tgtattaaaa gggggatttc
     5041 tgattggatc aaaatacggg ttagaaaacc gttcaaccgt tgatattgat acgacttatc
     5101 gaaattcgaa actaacaaaa gaaaagttag aaactgtttt ttctgaatta acagctaatc
     5161 ctacaaaaga tggaattcgt tttgaattaa agggattaac agagacgagg gaagccgatt
     5221 attatccagg ttttcaagca aagtttattg cttatcttga aaatgcacgt gtacctttta
     5281 aattagatat tacttcaggt gattctatta ttccagcagc attgatgtat gaacacaaat
     5341 taatgtttga agataaaacg attcatattc cagcttatcc tacggaacaa attctagctg
     5401 aaaaattaca tgccacgttt agttttggaa aagataattc gcgcatgaaa gatttttatg
     5461 acctgtatat gattccgaaa ttagaacgta tcgatgagaa aaatttgtat aagagtgttc
     5521 aaggaacatt caaggaaaga aataatcaat cggtgtttaa gcatttgtat gatcaggaaa
     5581 tgcctcaatt ggtgaaagat gagggcttga gagaaaagtg gaaaagatac caacaagaaa
     5641 actattttgc caaagacatt tctttcgaat ctacactaac ttccgttgat tccctgatgc
     5701 aatcaattgt agagcaagag aaggcagaac gaaaacaaaa ttttaagaga tcacaacaaa
     5761 tgaatcaaga aatggagaga taaagatgat caataatgta actttagtag ggcgattaac
     5821 taaagatcca gatttacgtt ataccgcaag tggcacagca gtggcgactt tttccctagc
     5881 cgttaatcgt aatttcacga atcaaaacgg caatcgagaa gctgatttta tcaattgcgt
     5941 gatttggcgc aaaccagcag aaacgatggc gacattagct aaaaaaggca gcttgatcgg
     6001 cgttgtcgga cgaattcaaa cacggactta tgacaaccag caaggacaac gtgtgtatgt
     6061 gacagaagtt gtggccgata acttccaact attagaaagt aaagcagcga cagaaagccg
     6121 tatgcacgct gatcaatcgt ccagcagtcc aagccctacc acctttgaac aacgggatac
     6181 agcgacgcct aacaacaatg gattgaacgc ttcacagaat ccatttggtg gacaatcaat
     6241 cgatattagc gatgatgatc taccgttcta agtagcattt tctattggtt gcatttgcta
     6301 cggtttatgt tataatttag acaacaaaaa gaattttcaa tctataacca aaaaccacta
     6361 gctggcgggc tagtggcttt cttttacgtt tctattataa gaattaacgg gtaggcttac
     6421 tgtctttctt ggcaaattta caatcaagcc atttgcagat atagtgcgcg gctacttctg
     6481 ccatgattgc aaaaacaaaa tcaaaaagaa ttttcaaacc tataacctcc tccccttgcc
     6541 agattaggcc ggggtagcag acagcaagac tattatagca taattgaaat atccaaatga
     6601 ttggattttt gctataattt tagcgaggtg attgtgatga acaaatatgt tggtttgctt
     6661 gataaaatcc gagtaataaa aacgcaaccg ttattggttc gctttacttt acaaacgatt
     6721 catgaatcga ttaattgtgt agttgcagat attgaaatta tcgataaatt gctaattatg
     6781 gatgatggaa aatacaatat tgccgtcaca ggtcacttca ataaacgaaa tcagctagtt
     6841 attgctagca tgtatgttag aaatcccgat cattttacgc gatcgatggg catataaata
     6901 aaagaagagt tgtagtccta aatgaggatt gcagctcttt ttgttttaca tagaaacagg
     6961 aaaagacctt tttaggaaga cacaagtgcc ttctcaattg aaaaaccttt ctctcactcc
     7021 aaccgccacc gccgttcgag aagaaaggaa gtgaacgctg cacgttcact aagatatggt
     7081 gtcttgcaaa gcaagacaaa atagacaaaa cttcctctca ccaaaaggtt cgttcctgtt
     7141 cg
//
LOCUS       DACGLW010000112         6565 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.118, whole genome shotgun sequence.
ACCESSION   DACGLW010000112 DACGLW010000000
VERSION     DACGLW010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6565
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.118"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            178..774
                     /locus_tag="IXT53_002637"
     CDS             178..774
                     /locus_tag="IXT53_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ4906407.1"
                     /translation="MTKAMVYKLLEEFNGTLALKDAKKAGISPVTIKRMVDRGELDRE
                     YPGFFTLPGQFPDELFMAQKKYERGIISHITALDLYDLTDMIPRQIDLTVPYGYHVSE
                     KGLKEFAVELHYTKAEWYELGKIEIKSRYGNPIIAYDPERTLCDIWNPWYTVEDEIKV
                     KAIKNYMESDRKNLRKLNEYRRILPTDKTMRSYIMALN"
     gene            787..1686
                     /locus_tag="IXT53_002638"
     CDS             787..1686
                     /locus_tag="IXT53_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HAQ4906408.1"
                     /translation="MTPTQLKAKVKNISREKEVDPQLVLRHFMMEKFLEKISESPYRE
                     NFVLKGGFLIGSKYGIENRTTKDIDTTLREMKVTKETLTTVLNEIFSTPTKEGIQFEI
                     QGMKETREADYYPGFSLRVLAHLENMRPDFKVDVTTGDSIYPATITHSHKLMFEDRTI
                     PLESYPTEQIIAEKLSATFDFLTDNSRGKDFYDLYTIPKMEKIDEKTLKDSVRNTFTR
                     RGKTDPLKIYYEKDMDVLKNSHELKDTWKKYQKSNPYAASITYDETMDSISNLMEKVI
                     HVEEKERKLAYQRHQQMNREMER"
     gene            1689..1820
                     /gene="ssb"
                     /locus_tag="IXT53_002639"
     CDS             1689..1820
                     /gene="ssb"
                     /locus_tag="IXT53_002639"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ4906409.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGN"
     gene            1842..2174
                     /gene="ssb"
                     /locus_tag="IXT53_002640"
     CDS             1842..2174
                     /gene="ssb"
                     /locus_tag="IXT53_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HAQ4906410.1"
                     /translation="MIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEV
                     VADNFQLLESKAATESRAHADQSSTSPSTATFEQRNTATPNNNGLNASQNPFGGQSID
                     ISDDDLPF"
     gene            2196..2453
                     /locus_tag="IXT53_002641"
     CDS             2196..2453
                     /locus_tag="IXT53_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906411.1"
                     /translation="MAKKISLTMDLLIPVIAFIDGISLGVTENPLVNRIAKGSLFLVF
                     VYLVCSYLLKRAGKDNSFWRLTKVTVAATAVILTAILPLYM"
     gene            complement(2612..2734)
                     /locus_tag="IXT53_002642"
     CDS             complement(2612..2734)
                     /locus_tag="IXT53_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HAQ4906412.1"
                     /translation="MKGVSLLSAYETIQTILGFGMFTIALISLIVNMLKNDKKK"
     gene            2924..3148
                     /locus_tag="IXT53_002643"
     CDS             2924..3148
                     /locus_tag="IXT53_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906413.1"
                     /translation="MIKTQPLLVRFTLRTSHEPINCVVANIEIADKLLILEEGKYTIS
                     VTGHFNKRNQLVIESMQIRNPDHFTRLMGI"
     gene            3596..3802
                     /locus_tag="IXT53_002644"
     CDS             3596..3802
                     /locus_tag="IXT53_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAQ4906414.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPYTVECDCGERAKCIKSYCLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            3802..4053
                     /locus_tag="IXT53_002645"
     CDS             3802..4053
                     /locus_tag="IXT53_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906415.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYENEYWLTKLEN"
     gene            4069..4506
                     /locus_tag="IXT53_002646"
     CDS             4069..4506
                     /locus_tag="IXT53_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906416.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYNETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGQWFFQALLCLEAGVKPNTIKPSEYQALE
                     LTYGKFVETKKAKTVSSEWLDYLENVNKYGACYIMKKEDNENE"
     gene            4499..5194
                     /locus_tag="IXT53_002647"
     CDS             4499..5194
                     /locus_tag="IXT53_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906417.1"
                     /translation="MNKVIYQLTTEDVSHFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYLESETSQTKVTVSNNYLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEELNSKDATVDDYYLVFTKFLTCGTE
                     SIVLMGMDYELAVPEVFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNELDRK
                     RMPYQRFERKKGQ"
     gene            5575..5754
                     /locus_tag="IXT53_002648"
                     /pseudo
     CDS             5575..5754
                     /locus_tag="IXT53_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5816..6000
                     /locus_tag="IXT53_002649"
                     /pseudo
     CDS             5816..6000
                     /locus_tag="IXT53_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003027804.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, HicA family"
     gene            6034..6465
                     /locus_tag="IXT53_002650"
     CDS             6034..6465
                     /locus_tag="IXT53_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HicB family protein"
                     /protein_id="HAQ4906418.1"
                     /translation="MLSEPLAKTYPAIFSPEEGGGYFIEFPDVQGAYTGINEDDISYG
                     IAMAQEVLGMVLADYIEHEDLLPEPTPINKISVEDDSFTTLIRVDVAKYLKDTELVKK
                     TLTIPQWADKLGKRAGINFSVLLTESIADKADNILRSGRNN"
BASE COUNT         2343 a         1092 c         1344 g         1786 t
ORIGIN      
        1 tcgttcagaa caggaaaaaa tgcgtgccag caaacgaaga ctgcagcaat tgaagaacca
       61 gcagaggatt caaaaagaaa tcgaattaga tcgataaaat cttcctaact attgtatacg
      121 tataaactta tgttatactg tttttataag tttatacgta tacaaaggag ggagaaaatg
      181 acaaaagcaa tggtatacaa gctgctagaa gaatttaatg gaactttggc tttgaaggat
      241 gccaaaaaag cgggcatttc tcctgtaacg atcaaacgta tggtagatcg aggggagctg
      301 gatcgagagt atccaggttt tttcacctta cctggtcaat ttcctgatga attgtttatg
      361 gcgcaaaaaa aatatgaacg cggcattatt tcacatatta ccgcgttaga tttgtatgat
      421 ttaacagata tgatccctag acagatcgat ctgactgtac cttacggtta tcacgtttct
      481 gaaaaaggat tgaaggaatt cgcagttgag ctacactata caaaagcgga atggtatgaa
      541 ttaggtaaga ttgaaataaa aagtcgctat gggaatccta ttatagctta tgatccagaa
      601 agaactttat gtgacatttg gaatccctgg tataccgttg aggatgaaat caaagtaaaa
      661 gcaatcaaaa actatatgga gtcggacaga aaaaatctta gaaaattgaa tgagtatcga
      721 cgaattttac caacagacaa aacgatgcgt tcttatatta tggctttaaa ttaaaggaga
      781 agatacatga cacctacgca gttaaaagca aaagtgaaga atatttctag agaaaaagaa
      841 gttgatccgc agcttgtttt aagacatttt atgatggaaa aattcttgga aaaaatctca
      901 gagtctcctt atcgtgaaaa ttttgtgtta aaaggcggct tcttgattgg ctcaaagtat
      961 ggcattgaaa atcgcacaac caaagatatt gatacgactt tacgggaaat gaaagtaacc
     1021 aaagaaacat taacaacggt tctaaacgaa attttttcaa cgccgacgaa agaaggcatt
     1081 caatttgaga ttcaaggaat gaaagaaacg agagaagccg attactatcc aggatttagc
     1141 ttacgtgtgc tggcacattt ggaaaacatg cggccagatt ttaaggtaga tgtgactaca
     1201 ggtgactcga tctatcctgc aacgatcact cacagtcata aattgatgtt tgaggatcga
     1261 acgattcctt tggaatccta tcctacagaa caaattattg ctgaaaaatt aagtgcaacc
     1321 tttgattttt taacagataa ttcgagagga aaagattttt atgatctcta tactattcct
     1381 aaaatggaga agatcgatga gaagaccttg aaagatagtg tgagaaatac atttacacgt
     1441 cgaggaaaaa ctgatccgtt aaagatatac tatgaaaagg atatggatgt actgaaaaat
     1501 agtcacgagc taaaagatac ctggaagaaa tatcaaaaaa gcaatcctta tgctgcgtcg
     1561 atcacgtacg atgagacaat ggattcgatt agtaatttga tggaaaaagt gattcacgtc
     1621 gaagaaaaag agcgaaaact agcttatcaa agacaccaac agatgaatag agaaatggag
     1681 agataaagat gattaataat gtgacattag taggacgctt aaccaaagat cctgatttac
     1741 gttataccgc aagtggtact gcagtagcga cttttaccct agccgtcaac cgaaatttca
     1801 cgaatcaaaa cggcaattga gaagccgatt ttattaactg cgtgatttgg cgcaaaccag
     1861 cagaaacaat ggcgacatta gctaaaaaag gtagcttgat tggtgtagtt gggcggattc
     1921 aaacgcggac ttacgacaac cagcaaggac aacgtgtata tgtgactgaa gttgtagcag
     1981 ataactttca actattagaa agtaaagcag cgacagagag ccgtgcgcac gctgatcaat
     2041 cgtccaccag tccaagcacc gccacctttg aacaacggaa tacagcgacg cctaacaaca
     2101 atggattgaa cgcttcacag aatccatttg gtggacaatc aatcgatatt agcgatgatg
     2161 atctaccgtt ttagtttgag aaagaaggag taagcatggc aaaaaaaatt agtctgacga
     2221 tggatctatt gatacccgta attgcattta tagacgggat ttctttgggc gtcactgaaa
     2281 atcctttggt aaatagaatt gcgaaaggat cactatttct tgtattcgtg tatcttgtct
     2341 gttcttatct tttaaaaaga gcaggaaagg ataattcctt ttggcgattg actaaagtca
     2401 cagtagcagc gacagctgtt atacttacgg ctattctacc gctttatatg tagagttaac
     2461 gggaaatcgt tgaacttctt gcgaaatatt gctataatga acatgaaaaa gaggagttgc
     2521 gctaacaacc cctcattaac agcaccgatt aaagaacggt ggcagaatat tagttatttg
     2581 gaaataaccg ctgacctgct aaagtagacg gttatttctt tttgtcattt ttaagcatat
     2641 ttacaatcaa actaatcaaa gcaatggtaa acataccaaa accaagaatg gtttgaattg
     2701 tctcatatgc ggacaaaagg cttactcctt tcatagattt tagcacttac attcataagc
     2761 accaccacct ttccagaagt cgccaccgtc ttcaaacttt actgtcttga ctattatagc
     2821 atatctaaaa tcgaataaaa attctcaatt ttagctgttt ttgctataat tttagcgagg
     2881 tgatcgtgat ggaacaatat attggtttac tcgataaatt agggtgatca agacacagcc
     2941 attgcttgtg cgatttacat tacgcacgag tcatgaacca atcaattgtg tagtggccaa
     3001 tatagaaata gcggataaat tgctgatttt ggaagaaggc aagtatacta tttcagtgac
     3061 aggtcacttc aataaacgaa atcaactagt cattgaatca atgcaaatta gaaaccctga
     3121 tcattttacg agattaatgg gcatataaat aaaagaagag ctgcagtcct aaatgaggat
     3181 tgcagctctt tttgttttac atagaaacaa gaaaaatgaa aaggcctttt taggaaggcg
     3241 caagcgcctt ctcaattgaa aaacctctct ctcactccaa ccgccaccgc cgttcgagaa
     3301 gaaaggaagt gaacgttgca cgttcactaa gataaggggt cttgcaaagc aagacaaaat
     3361 agaaaaaact tcctctcacc aaaaggttcg ttcctgttcg ctatctcttt attgatttgc
     3421 cagaccggca acaagttgcc ttttatcacc aaaaagcgac aaggacaagg gtaaatggca
     3481 cgctgcacgc ccttgtcctt gtcgctttta gttgattcat tctggccaac aaatcaataa
     3541 agaaagaact aacgaaagga agtaaaacct ttcctaagag gaggcagaat caatcatgcg
     3601 aaagaaattt gattattggg gtgttccctt cagcgaatta ttcccaaact atcatgcacc
     3661 atatactgtc gaatgcgatt gtggagaaag agcgaaatgt atcaaaagct attgtttgta
     3721 tcaatgtcca acatgcggga aaaaatatac cttgtcttat ggcgattata ttttgattga
     3781 tgagaaagga aagaaacgct gatgaaaaca caacacaaat tattgactgt aaaagaagtc
     3841 gttcaaattt tggtggacaa agaagtcatt tctgaaaaag tagcagaagc tttacttatt
     3901 ccgcggtact ggacgataga aaccccttta gtagaggatg caccgactat gctatttgaa
     3961 gacctagtga agattgcagg atatgaactg cattatcaag aaaacatgga aacttatgaa
     4021 aacgaatatt ggttaaccaa attagagaac taaaaaggag aaaaaaatat ggctcaaaat
     4081 tactatgacg aatttgtcaa attacctttg gataaaatgg cacaaaaaat ggaggatatg
     4141 acattcctat acaatgagac acgtgttcca aaaaaacatt ataaggaaag attgtcggta
     4201 gctgtggaag aaatgattga atcgggtgtc gaaatgaatc tcattgcgac ctactatcga
     4261 acgcttgaag aactgaaaaa gcaaaatgga caatggtttt tccaagcctt actatgcttg
     4321 gaagcaggcg tcaagccaaa cactatcaaa ccttctgaat accaagcttt agaactaacg
     4381 tatgggaaat ttgtcgaaac gaaaaaagcc aagactgttt caagtgaatg gttggactac
     4441 ttagaaaatg tcaacaagta tggcgcatgt tacatcatga aaaaggagga caacgaaaat
     4501 gaataaggtg atttatcaac taacaacgga agatgtgtca cactttttaa caaacaaaga
     4561 acatttgaat gaagtaatca gacggattca aaaagacttt agttgtatgg aagaaatgaa
     4621 gtactttatt caaagagtcg aagaacaatt gtatttggaa agtgaaacga gtcaaacgaa
     4681 ggttacagtc agcaataatt atttagacgt gtataaacgt cgtcctgaca cattaaaaaa
     4741 aggagcggag cttctacgac tagaaacaga aaaatatatc atgaatgttt acctagaaag
     4801 gagtgaactg gtcgaaaact ttgtcagcta cacgatttgc aggaaacact attactcaga
     4861 atcagaatat tattcaattt ttgagagcat ggaattaatg gaagagctaa acagcaaaga
     4921 tgcgacggta gacgattatt accttgtatt tactaaattt ttgacgtgtg ggacggaatc
     4981 tatcgtgtta atgggaatgg attatgaact cgctgtaccc gaagtatttt tgagggaata
     5041 tgtagaagaa gtagataacc caggcgacga ctggtacacg actttaactg attttttaga
     5101 tacctacact tacgacgata cagaagaaat ccaaaatgaa cttgaccgta aacgaatgcc
     5161 atatcaacga tttgaaagga aaaaaggtca ataaccattt ataccgaaaa gtgagaatgt
     5221 gtgaaataaa aacaaaaaac cttgaaatta agggaatggt tcttatttcg caccaatcgc
     5281 tccaaccgcc cccgccgctt aggtggtgcg aaataagcga gcaaagcccg aaaacctaag
     5341 agaaagaagg agatttatat gaacaaaatt attcccttat tattagtcct atttgttgga
     5401 gaaatactta tgcgacttag tgcagctgta catttaaacc tcgcagtatt tttggtaata
     5461 ctaatgtttt gtttgatctt ttggcgattt gtattagccc tcgtaagtct actattcaag
     5521 atgattgcag ccatattttt cataggatta tttcgatgaa aggagagagt accaatgcca
     5581 attgcaatag gaaataaacg gttacccgtg acgttggatg aaaagagaca aaaagaattg
     5641 cagtaaccaa agcagaagta cggcaaaagt gaatccagga ttatgtgtat tgcgttagat
     5701 ttattgattg cgcaagaaaa agcaggattt gacataccag cactcaaaaa gtgatcgcac
     5761 tttgcgcacg tgttaatacg tgttataatt attttgtaag gaggtatagt gcacaatgcc
     5821 acttacggga aaagaaatgc ttaagttatt gaaaaaaatg gctgggttga gcgaaggcaa
     5881 gagggatttc atcatcatct ttataaggat ggtgttagaa tcactgttcc tgttcatgcc
     5941 aatcaagatc ttggccgagg acttgagcga aaaattttaa aggatgcggg attgaaataa
     6001 tctcgcattc gattaaagaa aggattgact gaaatgttaa gtgagccttt agcaaaaacc
     6061 tatcctgcta tttttagccc agaagaaggt ggtgggtact ttattgaatt tcctgatgtt
     6121 caaggtgctt acacaggaat taacgaagac gatatatctt atggaattgc tatggcacaa
     6181 gaggtgttag gtatggtttt agcagattat attgagcatg aagatctact tccagaacca
     6241 acaccaatca ataagatttc tgttgaagat gattctttta ctactttaat tcgtgtagat
     6301 gttgccaaat atttgaaaga cacagaactt gtcaaaaaaa ccttaacaat tcctcaatgg
     6361 gcggataagc taggcaaaag ggctggcata aatttttcag tccttctaac agaatcaatt
     6421 gcagataaag cagataacat tcttcgttct ggaagaaata attagaactc aaaagggtca
     6481 gaaacttaaa aatctttggc ttcttttttt actacaaaga tggcttcttt aaattatttg
     6541 cctgattgat ttaaaaaaat tgcgc
//
LOCUS       DACGLW010000113         6173 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.119, whole genome shotgun sequence.
ACCESSION   DACGLW010000113 DACGLW010000000
VERSION     DACGLW010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.119"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            725..811
                     /locus_tag="IXT53_002651"
     CDS             725..811
                     /locus_tag="IXT53_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAQ4906419.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="IXT53_002652"
     CDS             complement(932..1621)
                     /locus_tag="IXT53_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906420.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1628..1906)
                     /locus_tag="IXT53_002653"
     CDS             complement(1628..1906)
                     /locus_tag="IXT53_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906421.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(1866..2780)
                     /locus_tag="IXT53_002654"
     CDS             complement(1866..2780)
                     /locus_tag="IXT53_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAQ4906422.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="IXT53_002655"
     CDS             complement(2762..3142)
                     /locus_tag="IXT53_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HAQ4906423.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="IXT53_002656"
     CDS             complement(3592..3879)
                     /locus_tag="IXT53_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HAQ4906424.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="IXT53_002657"
     CDS             complement(3899..4123)
                     /locus_tag="IXT53_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HAQ4906425.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4763..5296)
                     /locus_tag="IXT53_002658"
     CDS             complement(4763..5296)
                     /locus_tag="IXT53_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HAQ4906426.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..6159)
                     /locus_tag="IXT53_002659"
     CDS             complement(5317..6159)
                     /locus_tag="IXT53_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HAQ4906427.1"
                     /translation="MREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEKILSL
                     RFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQF
                     NELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQEL
                     TAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALALADKQ
                     KEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLLSDLD
                     NLFQ"
BASE COUNT         1633 a         1151 c         1042 g         2347 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaataaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtgtatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DACGLW010000114         6133 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.120, whole genome shotgun sequence.
ACCESSION   DACGLW010000114 DACGLW010000000
VERSION     DACGLW010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6133
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.120"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            complement(<1..476)
                     /locus_tag="IXT53_002660"
     CDS             complement(<1..476)
                     /locus_tag="IXT53_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HAQ4906428.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYY"
     gene            complement(617..1219)
                     /locus_tag="IXT53_002661"
     CDS             complement(617..1219)
                     /locus_tag="IXT53_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory regulator AgrB"
                     /protein_id="HAQ4906429.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            1464..1670
                     /locus_tag="IXT53_002662"
     CDS             1464..1670
                     /locus_tag="IXT53_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906430.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            1982..2701
                     /locus_tag="IXT53_002663"
     CDS             1982..2701
                     /locus_tag="IXT53_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HAQ4906431.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(2782..3465)
                     /locus_tag="IXT53_002664"
     CDS             complement(2782..3465)
                     /locus_tag="IXT53_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HAQ4906432.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            3683..5869
                     /locus_tag="IXT53_002665"
     CDS             3683..5869
                     /locus_tag="IXT53_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HAQ4906433.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT         2304 a         1026 c         1157 g         1646 t
ORIGIN      
        1 atatagtatg taaataatat caatgtcccg atcgtaccga tcatacggcg gtccacagtg
       61 tgaaaccaag tctctaacga tttttttaaa caaaaagcta ataaagcaga taaaaaagcg
      121 gataagacaa tgtgaaaata ctgcaatgaa tctcctggtt caataaccga agatttcaaa
      181 acaaaaaaat cgccgatgct tgttaagtga tctcctaaaa gcgacgtcaa taacccaaaa
      241 ggaagcgcgt aaaccaattg ttgacgcgaa gtcgaaaaga agaaaaaagt aagcatcagc
      301 attcctatag caattagagc agtaaaaatc cctaaaatcg aatagaccca tccaatcaaa
      361 atacaagaaa tcagaatgac tcccgcaaac agatatttgt atcggatgtg atggtcaatc
      421 aataagatac aaattgtgta aatcaaatat tgcaacgcta tggaccataa aaacataaag
      481 tacagctccc attgattttt accaaattat agcacaattg gcgtattatt tttttaattt
      541 ttcagggatt ttgaattcag aactcatacc aaagcagcag ccagatacaa aaaagtcaga
      601 taagttagtt aattgtttaa tgattgtttt tttcatgaaa gattcctcca aaattcatca
      661 agtagtttgt aataggaagc aacgtagcta tttctaaaac aatcccaagt agcaaatagg
      721 tagaaaagta agaagctgag aaaaataacg taatgagaga aaaaattaga aacatgctga
      781 tcgccttctt taataatggg ggacgtttct cgggttcaat gggattgatt gctgtacctc
      841 ttgatccata gcgaagcagt aacaaaaagc agctgattaa caaaaagacc atcattggga
      901 aggagatagt caattgcaga tgtttcatca ccaagggaac gccccataaa taaagtagtg
      961 tccacagcag acaaacgaat tcagagtcgg aatgcaatcc ataagcatat ctgcggatgc
     1021 agatataaga aagaaaggtg attcctgttt ccgcccatga gcctgtcacc agagaaaaga
     1081 ctagaacgac cgctagtttt ccaagattat tcaataaaac ttctaagaca aattttactt
     1141 ggacatacac catatcttct tccgatttgt ctccgctgaa taaggcttct aataatttgt
     1201 tcacaacttt atctttcatc tcatacgtac ctccaccaca aattttatgc taaaaaaaat
     1261 aaaaaacgtc ttttgtttca taaaaagtgg ttttgacatc aaaaccgtca tgatctccta
     1321 aaaaaatctt cgttttccaa gaaccttacc aaaaaaagtg acagttttga tacgaaaagt
     1381 actttttatg acaagtttgt gctttttcga gtgatttatg ttaaaaaatt actgacctta
     1441 taaaaggtga tggagggatt cttatggaaa atgaacaatt gtttgaagtc ggcgaaacat
     1501 atgcatttaa atctgattta tttgaatcac caataaaagg aaaaatagaa aaaatctatg
     1561 aaaatagtgt gatgatacgt gtcatgaaat gcgcagtaga agacaatgat accgctcata
     1621 atctgcactg gcagttagtt gtacgcaaat caagtacaat agaagaataa aaacagataa
     1681 aatgttgaga gcataaagag gttgtgagtc tcccgtttag ctctgagtta caaggaataa
     1741 tgttaaaaaa cagcttttca tgttttatga atattgcctt gtgaccgagg tgttggcagg
     1801 agaatactgt ttatttgaaa aaagggctgc gacaagactt ttgtcacagc ctttattttc
     1861 atgaatagga gttacttttg gctactttta gagaagtagc ttttaaggag cagataaagt
     1921 caaggctttt atttctataa atgaagtagt aaaatgaaga acagatttta ttacaggaag
     1981 gatgccgata attgtgatca ttgttgcagg aacgatcggt gcagggaaaa gcacactaac
     2041 tgaaatgctt gcccaagatt tagaaacaaa acctttttat gagaacgtgg aagataatga
     2101 agtattgccg ctattttatt ctaatccaga aaagtatact tttttattac agattttctt
     2161 tttgaacaaa cgctttttgg caatcaaaga tgctttcagc catgatgaca acgtactaga
     2221 ccgctccatc tacgaagata gtatgctgtt tcatttgaat gcagatttag ggcgtgtttc
     2281 tgaagtagaa gtaaagcagt atgaaggtct tttagaaacg atgctgaagg agttagaaga
     2341 aatttcgcca caaaagaaac cagatctact ggtttatatt cgtgtatcct ttgaaactat
     2401 gttagcgaga atcaaaaaaa gaggccggga atatgaacag ctagaacaag atcctgaact
     2461 ctattcctac tataaagaac tcaatcgacg atacgaagag tggtatgaac agtttgatat
     2521 atgtcccaaa atagtgatcg atggggacaa atatgatttc gtagccgatc cggcatgtgg
     2581 agaacaaatc gtacaagaaa tcaagatgcg ggcaaagaaa atgacagaag aggcagatgc
     2641 ccagtcgatc cgctacacta caaataaccc gaaagcaaag ggaatcttta caactaaata
     2701 atataaaaac taagcaaatt gctcactcgc agatatttct ctgtgagcag catcttgctt
     2761 agtttttttg attcattgag atcaaatagt gaaccgattc gtatgataaa attgccataa
     2821 atgaaatgtc tgagtgaggg ccatgatcaa aaataaaatg gaaacgatca aaatcgaatg
     2881 ggcgccaaac agatgatgac agaaaacaag gacaatagct cctgcgataa aggcaagaac
     2941 gacacttaga tagtggaaaa atcgatcaaa atctgctttt tctttagaaa tacaataatc
     3001 atataagttc gttgctaccc cttttaaatt tccagttgta aataaattag tgtaggcacg
     3061 cccgttgatt ttatcaaaag ctacccactg gatcgatgca gtaaaagcaa tcaaaatcgt
     3121 tactaatgcg ggatgctcat ggacgggtgt caaactaaca aagaaaaaaa tcactgcttc
     3181 ataatataga acaaacaagc gccagaaatg tttttctttt tttctaaaat aatcaattag
     3241 atactttgtt agaagaatcc ctaagaaaaa gaaaagggta gacaatactt ttttaccaac
     3301 acttagccat tctccgtcaa atgcttggat gatcgataga atgatattgc cagtttgagc
     3361 agaggcaaaa gcaccatatt ggatataagt ataactgtcc atggctcctc cgataaatgt
     3421 cagcaaaagt cctattgtac gcccctcgtg aaaggaagtc tgcatttttt tcatcacctt
     3481 ttatgaatat ttttgtcttc cttatcttag cataaacgat tgaaaaaaag gaaaaatttt
     3541 cagaaaacta aaatcatatc gcttcccatt ttcattttta gaaaggtctg ttagtataaa
     3601 gaaaaaatag acaacgaaac tttaaataac caacttgaaa taaacaaatt caaagaagaa
     3661 agatgggaag gaaatcaatc agttgaatga aaaagaaatt ctccgattac aaaaagaatt
     3721 gaaacgctgg gagaacaagt ataaaaagct tcaacaagtc tttcggctac aagaagaaat
     3781 cattgctacc tatgaagcgg cttatggcga agcacccgga cttattgagg aagaagtaca
     3841 agtaaaagaa agtgaaggaa acagctcttt ctttaaaatg atggagaaac gttcctataa
     3901 tcgcttcaat caagaacaaa aagcagctat cacatatgat atgaagaaac atcttcgaat
     3961 catagcagga gctgggagcg gcaaaacgca aacaatctgt gcaaaagctg tttatctgat
     4021 gacgcaaaaa caagtagacg aagaacgcat actaatgatc acttttacaa ggaacgctgc
     4081 aaatgaacta aaaaaacgag tagataattt cagccagaga aaaaccgctg tccatatcgg
     4141 aacattccat agtatctttt ttaggatata caatgaaatt tgtcgaaaat ttcctgaagt
     4201 agcaatgcaa ggaatacaag gagacttttc aaaggatact gcacaaaaag tgaatgctgt
     4261 tttgcaacag ctgatacgta aatataatct ttatatattt gatcaatatg gagaaaaaac
     4321 gattgcttct cggttagact attggcagaa tatgaacctt tcagtagaag aaatggttca
     4381 gttagttaaa gaaaaatatg attccattga taagaatgca cgacagccga tatcagaacg
     4441 actgtatgga ttgctcacgg aactccaaga acaaaaaaga agccagcaat tgctagagtt
     4501 caatgatgta ctccaaaatc tgaaaagcgc tttggagaat gaggagatcc agcattatat
     4561 cggccaaaaa tatgattatc tattcattga tgaatttcaa gatactaatc cacttcaatg
     4621 ggaaatcgtg aaacggatga caaaggaaaa caggatcaag ttgattgttg ttggagatga
     4681 tgatcagagt atctatggat tcagaggatc agagccagca tacatcaaga actttgaaaa
     4741 agaatatcct accaaaactc ttttcttatt aactaattat cgttctcggg cagctattgt
     4801 ccaaggagca aatagattga tcagctataa taaaaatgat cggataccaa aggcgatgat
     4861 tccagcacaa aaagaagcag gtgttatgga agcctatttg ttttctgata ctaaagcaga
     4921 aagtcagtgg ctaatcaaac aaattcagac atttattcaa aaaaatggga aatataagga
     4981 atcaatcata ttataccgat cagcttcaca aacgcagcag cttgtccaat ctctgctgaa
     5041 aacgaatatt ccttttgtac tggaagcaga tagtccatat gaaggtatct ttggtatcaa
     5101 gtctttccaa catttctatc aaaaaattgt tcattggcaa acagcactga atctgcaaaa
     5161 gaaaaatcag gcctaccagc aattgctccg gcagctgatg gcagactgct acctgaaaaa
     5221 aagtgaatgc gatcaatact tttctcctaa atatcaaaat atagccgttg cagattatat
     5281 tttgtcgata agccccaatt taaaatcgaa atctgcagaa atccgagagt ttgaggaagc
     5341 gttgcgtaaa acagaccagc atcaagaaat ctattcgctt gttcaaagct atcttcatct
     5401 gccacgtata gctaaggaaa tcgaccagag tgatcaagaa tggatacgag aagaagtaaa
     5461 gcagcatgct actttcagaa gactccaagc cttatcgcaa gaaacccaag cgacagctaa
     5521 agaattaaaa acccgcttga ttgcttacag acaaggaaaa cttgatgcac tatgtatcca
     5581 gagcattcac aaaagtaaag gtcttagtta ccgaaatgtc tttttgattg gttgtaacga
     5641 aggaagtttg ccttataacg gagcgacaga aaagaaagta atcgacttta atgaaataaa
     5701 agcagaacca gtaacgacga tagaagaaga acgaagacta ttttatgttg cgatgactag
     5761 agcaagaaat cgtctgtatc tatgtgtacc tcaaatgaaa aattcaagaa aattgaaagt
     5821 atcgagattt atcaaagaaa ccgggagtcg tgtgaaaaaa ggaaagtgat agaagagagg
     5881 gtgtgacaga agtggtcagt ttcaagaaag aagaaggaat gtacgaaaat tgcttctcaa
     5941 atttttgtga aatttcagct tattttcgaa gaagctattt ctggaacaca gtttattcgg
     6001 aaaagggagg tgtgacaaga attttgtcac acctccctta tttattttga aaatgagaat
     6061 actattatca aaatagagaa tatatagaaa aattctcatt attcaaacgt aaataatcag
     6121 ttattttgat ttt
//
LOCUS       DACGLW010000115         6112 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.121, whole genome shotgun sequence.
ACCESSION   DACGLW010000115 DACGLW010000000
VERSION     DACGLW010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6112
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.121"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            58..510
                     /locus_tag="IXT53_002666"
     CDS             58..510
                     /locus_tag="IXT53_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906434.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            524..1213
                     /locus_tag="IXT53_002667"
     CDS             524..1213
                     /locus_tag="IXT53_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906435.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1338..3689
                     /locus_tag="IXT53_002668"
     CDS             1338..3689
                     /locus_tag="IXT53_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HAQ4906436.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3769..4164
                     /locus_tag="IXT53_002669"
     CDS             3769..4164
                     /locus_tag="IXT53_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906437.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            4489..5106
                     /locus_tag="IXT53_002670"
     CDS             4489..5106
                     /locus_tag="IXT53_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ4906438.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            5147..5446
                     /locus_tag="IXT53_002671"
     CDS             5147..5446
                     /locus_tag="IXT53_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906439.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            5480..5647
                     /locus_tag="IXT53_002672"
     CDS             5480..5647
                     /locus_tag="IXT53_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ4906440.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(5692..>6112)
                     /locus_tag="IXT53_002673"
     CDS             complement(5692..>6112)
                     /locus_tag="IXT53_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ4906441.1"
                     /translation="SLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGNELLD
                     TAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRLV
                     FKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT         2110 a          837 c         1226 g         1939 t
ORIGIN      
        1 caattatctt atcgtagata tatattataa atttaataag aaagcgaggt gagaatcgtg
       61 aaaaaaacga tactaagtat tttttcagta tttacaatat taattggggg ctattttctt
      121 ttgaatttag attctgttaa ttcaaacgct caaataaaag atcaaagagt agctgaacat
      181 aataataaaa aaatgagttt gaccaaaaat atctattcta tgtcagatga agaattgtat
      241 aaagaatttg gtgatttatc cggggaacag ttagaagaaa gtattccagg aattagtgac
      301 tcagataaaa ttgttgtctt gtctgctggt gggcaattaa tgggagatgg caaatccgaa
      361 ttagttagtt ataaacaaaa tcacagacag tttattgatg ttaatactga ttccaacgct
      421 atttcgatta aggatctaaa agagctttta aatagtaaaa gaaatgaatt aaagcagata
      481 aaagaaacta attcagtgga attagagtaa aggaagattc attatgaaaa agatttttgg
      541 tgtttctgtt gggctactta tgatgttaac atttggaaat gcagtatatg ctgctgagaa
      601 agaagttatt tcagatccag agcagtacgc ctcacgatac tttgctgatt ttgaagaaga
      661 agataaagta cttgcattgc cagaaggtgg ttttttaaaa ggggaagcta aaattgtatc
      721 tatggataat ccagatataa ttcaaggttc atataattca gaaactgatc ctgcttcagt
      781 ttcagtgaca gaagcaaaaa aaattgttgc tgaaaaattc aaaaatgata ttgaaactac
      841 tccaattatg cctcgaggag catttccacc cgatgttggt actagcggac accttcttgt
      901 actagcagga ggaggaagct ataaatcaca gccatttagt ggtgcgggat ggcgttttgg
      961 aggtatgggt gtaaaaccag ctgatggtac aggcaacttt ttacgttgga aatcctacat
     1021 tgacgatgga agagtaggaa atgtaaatca agccaacgca actaaatctg gttctattca
     1081 aggaatggca ttgtatgctc gtgaaggatt caaatacttt gcaccaaacg ggcctggttg
     1141 gacaatgaat ataggtattt attacactta taatcctgtt gcaggtacgt attatgttgt
     1201 tcaaaatatt taataaaaca gcacaaagag atttctttgt gctgttttat tatttttagt
     1261 tgttgacaaa cactaaaata taaaatataa tcaacacata aacgaacgta tgttcggcaa
     1321 gaaagcaggg gatttttatg gatgagatgg ttttaagtac tcagaaatgg ctaaacaaga
     1381 aatacagtaa tgttacaggg tttgataagg ttccagaaaa tgggcgaaca gggtggccta
     1441 caatctatgg acttattgaa ggactacaag tagaacttgg tattactaat ctagtagcta
     1501 attttggacc taccactgaa aaaatgtacg ataatcaggt tactcctaaa tggggcaaaa
     1561 atctacctaa aaatattgta tttcttattc aaggtgcctt ttggtgtaag gggattaatc
     1621 caggtggctt cgatggggta tatactccat atttagatac tgctgtaaaa gagttgcaga
     1681 ctgatgcagg gtttaatgga agcgctgtaa ctggagtttt ggatgcgaaa tgggccaaag
     1741 cgttatttga tatgagtgct tttgttcttg ttcccggcgg agatagtaaa attagaaaaa
     1801 tgcagcaatg gttaaatgta aattatttag aatacacggg aataatgcct tgtgatggta
     1861 tttatcaaag aaattcaaat caagcattaa tttttgcatt acaagctgaa ctaggatatt
     1921 ctcccagtga agcaacaggg tcttatggta atgggacaac tagtaaaact tatcctgtgt
     1981 ctgaaggtaa ctcgggaaac tatgtaagaa taattcaata tggattatat gttaatggtt
     2041 attttgaaga cggtacattc gatggtattt tcactaaata tatgggttta gaagtactgg
     2101 cattccgaaa atttatggta ttgcctgaat atactgaaat agcagatgtt gttgtaataa
     2161 aaggactatt gtctagcgct ggagatatta gaagatctgc agatggcgca gatacttcta
     2221 cgcaattaac tagatctcaa atccaaacat tggttgataa tgatataaaa atagttggta
     2281 gatatcttac tggtactgtt ggaattggaa aagatgaacg aaataagtat ctaactacag
     2341 aggaattgaa taatattttt tctaaaaatt tatctgtgtt tcctatctat caagatggag
     2401 gtgcagcatt agcttatttt acttatgatc gtggcttatc agatggtaag aaggctatag
     2461 atgcagcaaa aaatctaaat ataccattaa caacagttat ttattttgct gttgatctag
     2521 acatgttagg ggaagaaatt cttgcatatg ctggagaata ttttaagggg gtatctgctg
     2581 taatgtcata tagcgggtat caaacgggtg tttacggaac aagaaatgtt tgttctcaaa
     2641 taatcaataa cgggtatgct tcgtttgcat ttgtttcaga tatgtcgact ggttatagtg
     2701 ggaatttagg ttttcctatg cctagacaat ggtcttttga tcaatttgtt gaatttacca
     2761 taggaagtgg caacggagct gttggtatcg atttaatcgc tacatcgggt agagatagcg
     2821 gatttaatga gttatcaaat gatactaata atgataatta cattgccaaa tacaatcaga
     2881 aggttataaa tcagatggta cgtgcgtatc aatatctatc acaagctggt cttgataatc
     2941 cttggaaccc tcatttgaca ttttatagat atgtaaatta ttctggtttg tcatgggaca
     3001 tcatttcttc tcctgtaaca gagcatgata gaaaaattta tgatgaatat aggaataaat
     3061 taactaatag cgagggcctg tataattatt ttatagatcc taatactggt tcaataatag
     3121 gtttgcctca cttaattgtg acgttacaat cacagatgtt tctagccgac acaatcaatg
     3181 atagtgtttc tgactttgca ggttggttag gagatttaat gacttgctgg ggtgaagtta
     3241 agaaattagg aatacaaatg gagcaaggtg tatttgcatt agtaggtacg gctgcaggtg
     3301 gaacatttag tctagaagat ttataccaag atgttgacgc ggtaaatgtt tataccttag
     3361 caaaaagaaa ttctgattca acagctttat cggctttaaa aacgtactat tgtgataagg
     3421 gtgctattga tagatttaat caatttgtta agaacagatt tggttcttca gataaaatcg
     3481 cttcagtaac aaatagttta ttagaaaatg acgaaacagt tgatatctta ggtgtaggga
     3541 taatttcatt tttcattgat aaaatagtta ataattcact tgggacagat tttacttatc
     3601 ttgattttat tggagatgag gggaaagttg caaatggatt ttctgaaaaa attattcaat
     3661 ttcaagaaaa tagaagtaaa cttaattaag aatcgtttac tatttaaaca gtgtcctttt
     3721 cctttttaaa tatatacgat aaaataagta tatgaattgt aaaaggacat gatgattatg
     3781 gaagaaatag aatggaataa tctgactcaa aataataaaa ataattctgg atgtggaatt
     3841 atctttatgt ttgttatagt tttgggaata gtagtacttt taataactgt caagatagta
     3901 gattactttg cgaatactcc tgaagaacaa atacagagaa aacttcctga gtttgaaaga
     3961 gtaccacctt atgatccaag agataattca tttcaagtaa aaaaattaaa agagtctcca
     4021 aatgaaatgt ggtttgaagt tacagttgaa tcaagcgact tcaaagaaaa aaagaaagaa
     4081 tacaattata ttgtaaaggc aaaagtatca aaaagattta tttttaaccc taaattagaa
     4141 atatatagtg ttgtaataga ataatagtgt taaaaaaatt gtaaaagtta aagaagtcaa
     4201 attctgatgt gaaaaataaa atataccatg gaagacataa aagaaaaact ggataactag
     4261 tttatttaat ggcagaccgt taaggcctgc catttttatg tattttagcg gaataaatat
     4321 aatcatgcgg gagtgtaaac ttactgtttc aaaaagtata ctttttgaca tgccttgatt
     4381 ttctttgttt atgtatggca taataaccac ataatacaat ttgaaacgtt tctgatttaa
     4441 tctgattgtt ttgaaacaat tttagtgaaa aaagggtaga taaaaacgat gagaaaattt
     4501 ttttatatcc gggtcagtag ttatgatcaa tcgatcgaac gacaactgat ttccgcgaag
     4561 gaattaggaa tccctgaaga atatatattt gtagaaaaag ccagcggcaa agattttaaa
     4621 cgccctgaat atcaattaat gaaacggatg tttcgaaaag gagatttact ctatctacaa
     4681 tcattagata gactaggcag aaataaacaa atgattttag aagaatggcg agaattaact
     4741 aaggaaaagc agattgatat ttctgtgtta gacatgcctc tgttagatac tgctagttac
     4801 aagcaactag atgggttaga gacgcttatc tctgacttaa ttctgcaatt actcagctat
     4861 atggccgaag acgagcggaa acgaattaat agccggcaaa gagaaggcgt agaagctgcg
     4921 aaacaaaagg gtgtaaaatt cggcagaaag aaaattgctt tacctcctga atttcctgcg
     4981 atctattctg actggaaagc aggaaagata actgcagtct atgctatgga tcaattgggc
     5041 atgaaacgaa atacctttta tcgacgggtg aaagaatatg aacatacctt gcaccacttt
     5101 tcttgataga tataacagct attagacggg aagggagagt ttgaaaatgg acttaacgaa
     5161 tcaaagggtc gtttttactg gaacgctgca gcaaatgaca cgaacccaag ccatcggttt
     5221 ggttcactct ttaaacggcc attgtcaatc aagtgttact tcaaaaacaa atttcctggt
     5281 ttgtggtcag tattctaagt cgttgtttga tgattctctc tatacaaaaa aggaacaaac
     5341 cgcgcgggac ttgattgcgt taggtcatga gattaccatt ttatcagaag tcgaatttta
     5401 tgcgttaatt agtcaacagt tgaaagaatg gcaacggtat ttataatcgt tagtaaaatt
     5461 tacaaggagg aaaattttta tgaagcgaaa atcttatgat cgttggggca ttgaaataac
     5521 tagttttaat cgaaatattg cttttcacgt ggagtgtgac tgtggggaat tagcaaaaag
     5581 tgtttatcaa aaaaattact tttactgtcc taaatgccat cgccgatacg gggtacatgg
     5641 aacatagaaa cgaaatagtg cggtagaaaa gcgaatgaat gtagtatatt tctacaaatc
     5701 attcgctttc tttattttat aaatagttgg tcgagagatc ccgttttctt ttgcaatctc
     5761 tgcaactgga atgccttgct ttaacatgtc caccacggtt ttaaaaacta agcgtttttg
     5821 cggatctgtt gagttaatag aatactcgat tggtcgtccc ttatatcggc cggctttttg
     5881 agccaataat actccttgcc gctgacgttc acgaattttc tcacgctcgt tttgtgcaat
     5941 ataacttagc agtgataaaa acatatcgaa catagcggta tccaggagtt catttccagt
     6001 attaaaatct aaaaactgtg catctaatat ttttaacgct acttttttct gtttcataaa
     6061 agcaactgta ttttttatat cttcataatc tcttcctaaa cgatctagtg at
//
LOCUS       DACGLW010000116         6020 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.122, whole genome shotgun sequence.
ACCESSION   DACGLW010000116 DACGLW010000000
VERSION     DACGLW010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6020
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.122"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            585..638
                     /locus_tag="IXT53_002674"
                     /pseudo
     CDS             585..638
                     /locus_tag="IXT53_002674"
                     /inference="COORDINATES: protein motif:HMM:NF019230.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            642..1241
                     /locus_tag="IXT53_002675"
     CDS             642..1241
                     /locus_tag="IXT53_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HAQ4906442.1"
                     /translation="MREKNKNSSRYLVATVVFVCVLIFLFDFLQIGKSIIGNTLSAKE
                     MVALGGLYEGHLFPNIFISIVSHINLYHLISNLLFLVVLEEALNDQYEWLTLLIVLII
                     SGFLGSLVPFLLHSNIVSMGLSGGIFGWIALFAIAYIRFDSKKHLLLSVFVLLIIGIL
                     LVSTFESGEANVLSHVIGFVCGMILGFILKNVSLIKQKN"
     gene            1257..1733
                     /locus_tag="IXT53_002676"
     CDS             1257..1733
                     /locus_tag="IXT53_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906443.1"
                     /translation="MPKRKEKKIPVFLSVSEMLSLQILLRKKIETIDEEVRNKRAMYE
                     QREALRERIHYFDDIHTKIYEARTNHEDESKLGLAQQRILEWLNTNTDQEPLRVISNL
                     FEFGTKHWFNAVEKDADLQEAFDTLSKDSKSMRDLLITYLNEHEFEQTKTKKKNKE"
     gene            1750..2652
                     /locus_tag="IXT53_002677"
     CDS             1750..2652
                     /locus_tag="IXT53_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906444.1"
                     /translation="MLLTLLGYLCLILAFFGAIAIIIGQFQTNNPIMRNIGIGIVIVT
                     FIVFNATKIFGSNDKQFGISGVNNSYAPDSLGRVVIDGTFENVTSYKNVSLTVKEDGK
                     KASDDVLATIKPSQSQFEVFYTVKENSPVHKLTLVFNVDGEEYYKDVKINKTSTKKII
                     ESAKKEVATEESTTESSSTVEAELPDKIDFDPEKVKSFESVSGSMADRFNTEVFKAIK
                     TVKLDTEKQMINVTLFNSVESMDQDKKVTVARELGKRVIGLYRGTAKPDSDGADLPVH
                     VVYEDGKVFGDSTANEALTIKLAK"
     gene            2725..2916
                     /locus_tag="IXT53_002678"
     CDS             2725..2916
                     /locus_tag="IXT53_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906445.1"
                     /translation="MVVNKEKVRKYVTLKIASVKYLEKRAKEEERARGKKVTVSELLE
                     ELIENDQTIRRAFTNRSIR"
     gene            3080..3667
                     /locus_tag="IXT53_002679"
     CDS             3080..3667
                     /locus_tag="IXT53_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906446.1"
                     /translation="MENNNDIRHFSIGERVTTVSELSKDYRDKEGTIISLNKNRRGVW
                     QYVVDLGDGVTVECKDSQLIRNNGSHSIEKAILVYPKVSSKIMNDWLLGKFGEYNKKE
                     IAEVIGKSYPTVLGYLKKITSKEAKIEDVLTVRELQSLIEYRNKQITNPEYLRKEDGL
                     LKQLEQVNKIRKMQKVAPLSLDTFKKIYLKEIKKD"
     gene            3667..4566
                     /locus_tag="IXT53_002680"
     CDS             3667..4566
                     /locus_tag="IXT53_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HAQ4906447.1"
                     /translation="MWTVTLPFQPVSAPRPSARRNAADETNDVAEKRISTYYDKKYLD
                     YLKKIKSFIEDQDLLDDEFYSIVNDPMGAIMEVDFYYQIPKSYKKVYNIMKTTAPDLD
                     NLVKSAMDGIFNISAIRDSHITGLIAFKYNVANEGKTVVRIKRYSEFEWDTSEGLNGD
                     IWEATFDFKPVATPRPKSKFRGNGIITYYPKEYNDYLENIKNTLKEKDLVNDQLKKVM
                     NAPMGVIAQIDYFYQVRKDKKKLDSLMRTSQPDIDNLVKGTLDGIFNKEIGIKDSRVV
                     GLIAFKYNTFEKSKTNVRLQEMG"
     gene            4566..5480
                     /locus_tag="IXT53_002681"
     CDS             4566..5480
                     /locus_tag="IXT53_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906448.1"
                     /translation="MNNNEKILHVLDSFEIIQEELKKYRDVLEQRYDFVNQQKSNHMD
                     FILNMNDLKKKLVERKEQEKLIKAYFELGEKEVKNAMELNEERRVLDQLLEQLLVMFQ
                     KGRIDEDLIEEGLRKYPSNSGIGIVLKAIDEEEIEAFIPPEDFESAMEYIKYYSQGIT
                     AFREFDPEDVIHDLNNLKEWCESYGVDDSGLDYLISIMEIEEEMPDKPDPTDILELIH
                     EARNPIAYISRGYTVLEYYKPYISAMNHLRRVLREKREYRSVLNATNRLEKAVSELDA
                     YYREHYLQAGGMPRNTKANISRYIKKAE"
     gene            5532..5747
                     /locus_tag="IXT53_002682"
     CDS             5532..5747
                     /locus_tag="IXT53_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906449.1"
                     /translation="MKRDVFELYVLERLEDGMMAQEIIDEIDDMISRFGQLELNDGNK
                     NSVVLKQFWLNNFSYKDFIKEKANTLY"
BASE COUNT         2306 a          769 c         1210 g         1735 t
ORIGIN      
        1 aaccttggtc acaggcagaa attgataaag cagtaaaatt gaaccgatta tcaaaattta
       61 acgtattagg aaatgccttt taaaaatcgc aatacacaaa cagaaaaata ccaaaaaatt
      121 agcaatgaac gtaggtaaga acatgacaat aaataataat aaccctaaaa aatataatgc
      181 tcttgtccaa tgctatatcg aaaacaaaat tacaatgcaa gaagtcgctg atgaattagg
      241 caaatcaaag agcctagtat cacacgattt taagcacaat gtttttccga acccgattga
      301 gctgaggaaa aaatttcaaa agagagttta tcaagaaata ggtaagtgga ttgatgaagg
      361 gaatgcaatt gaccctttaa ttgaaaaata tcctttttta aaaagcaaaa ataaaattca
      421 aaggttaatt cgtcaaggcg atattcaagc taaagaacct gttttgacaa ccaatgattt
      481 ggctaaaata aatcttcata aacaaattat tgaagacttt gaaaaaaatc tttcaacaac
      541 tgaaatttta aaaaaataca atattccgag aggaaagcta acatcgactc tttttagaga
      601 atatggagaa tcggcagtta ctaaagcaaa ggaagtagcg aatgagagaa aaaaacaaaa
      661 atagtagtcg atacctagta gcgactgtag tttttgtttg tgtattgatt ttcctgtttg
      721 attttttgca gattggaaaa agcataattg gaaatacatt gagtgcaaag gaaatggttg
      781 ctttaggtgg tttgtatgaa ggacacttgt tcccgaatat attcatttct atcgtttctc
      841 atattaactt gtaccattta atttctaatt tattgttctt agtagttctt gaagaagcac
      901 tgaatgatca gtatgagtgg ttaacattat tgatcgtttt aatcatatca ggatttctag
      961 gaagcttagt tccttttctg ctacacagca acatcgtaag tatggggctt agtggaggta
     1021 tttttggatg gattgcttta tttgcgattg catacatccg ttttgatagc aagaaacatt
     1081 tattactaag tgtttttgta ttattgataa tcggcatttt attggtatct acttttgaat
     1141 caggcgaggc aaatgtcttg tcacatgtaa tcggatttgt ctgtggaatg atcttgggat
     1201 ttattctaaa gaacgtttca ttgatcaaac aaaagaatta aaagggagtg ctaaaaatgc
     1261 caaaaagaaa agaaaagaaa attccagtgt ttttgtctgt ctcagaaatg ttatcacttc
     1321 aaatactgtt aagaaaaaag attgaaacaa tagacgaaga agttagaaat aaaagggcta
     1381 tgtacgaaca acgtgaagcg ctaagagaac gaatccacta ttttgatgat atacacacta
     1441 aaatctatga agcacgtaca aatcacgaag acgaaagtaa attaggatta gctcaacaac
     1501 ggatacttga atggcttaat acgaatactg atcaagaacc gttaagagtg attagtaatc
     1561 tttttgaatt tggaacgaag cattggttta atgctgtaga gaaggatgct gacttacaag
     1621 aagcgtttga tacgctaagt aaggatagca aatccatgcg agatttattg attacttatc
     1681 taaatgagca tgagtttgaa caaacgaaaa ccaaaaagaa aaataaagaa tagaaagttg
     1741 tgatgaatca tgctattaac cctgttaggt tatttatgtt taattcttgc tttttttggg
     1801 gcaatcgcta taattattgg tcaatttcaa acaaataatc cgattatgcg taacataggc
     1861 attggaattg tgattgtaac ctttattgtg tttaatgcca ctaagatttt tggaagtaac
     1921 gataaacaat ttggtatatc gggagttaat aatagttatg ctccagattc tttgggaagg
     1981 gtagtaatag atggaacatt tgaaaatgta acgagttata aaaatgtttc gctgacagtg
     2041 aaggaagatg gcaagaaagc atcagacgac gttctcgcta ctattaagcc gtctcaatca
     2101 caatttgaag tgttttatac agttaaagag aatagccctg tacacaagct gacattggtt
     2161 tttaacgttg atggagaaga atactataaa gatgtaaaaa taaataaaac atcaacgaaa
     2221 aagattatcg aatcagcgaa aaaagaagta gctacagaag aaagtacaac tgaatcaagt
     2281 tctactgtcg aagcggaact accagataaa attgattttg atcctgaaaa agtcaaatcg
     2341 tttgaatctg tttctggttc tatggctgat cggtttaaca ctgaagtctt caaagctatc
     2401 aaaactgtta aattggatac tgaaaagcaa atgattaatg ttaccttatt caattcagtt
     2461 gaatctatgg atcaggataa aaaagttacg gttgcaagag aactaggaaa aagagtaatt
     2521 ggtttgtatc gtggtactgc taaaccagat tcagacggtg cagatttgcc agttcatgta
     2581 gtttatgaag acggtaaagt ttttggagat tctacagcaa acgaagcatt aacgattaaa
     2641 ttggctaaat agccagaaag ttataataaa caaatggtgt cacaccaata atatataggt
     2701 gtgacacctt ggagatgata gctgatggta gtaaataaag aaaaagttag aaagtatgtg
     2761 actttaaaaa tagcttctgt taaatattta gaaaagcgag ctaaagaaga agaaagagca
     2821 agaggtaaga aagtaacagt ttcagagttg ttagaagaat tgatcgagaa tgatcaaaca
     2881 ataagacgtg catttactaa taggtctatc agataacaat ataattaata aaaaagaaag
     2941 agtgatttgg tcttagacta aatcactctt tttatataaa gtttctttac actcattccg
     3001 aataatgtta aaattcttta tatgatatat agttccttgt cagaggaaga gataaccagc
     3061 aatattggag ggataataag tggaaaacaa taatgatatt agacatttta gcattgggga
     3121 aagagttaca acagtaagcg aattatcaaa ggattataga gataaagagg gaacgattat
     3181 ttctcttaat aagaatcgaa gaggcgtttg gcaatatgtt gttgatttag gtgatggcgt
     3241 tacagttgag tgtaaagata gtcaactgat cagaaataat ggcagtcatt ccattgaaaa
     3301 agcaattctt gtatacccaa aagtatcaag taagatcatg aatgactggt tgcttggaaa
     3361 atttggtgag tacaataaaa aagaaatcgc agaagtgatc ggcaaatctt atccaacagt
     3421 attagggtat ctaaaaaaga taacgagtaa agaagcaaaa attgaggatg ttttaacagt
     3481 aagagagctt caatcattaa tcgagtatcg gaataaacaa ataacaaatc ctgagtacct
     3541 tagaaaagaa gatggtctat tgaaacaatt ggaacaagta aataaaatcc ggaagatgca
     3601 aaaggtagct cctttaagcc ttgatacctt taaaaaaatc tatttgaaag agatcaaaaa
     3661 agactaatgt ggactgtaac tttaccgttc caacctgtat ctgcaccaag accaagtgca
     3721 agaaggaatg ctgctgatga aactaatgac gtagcagaga aaaggatttc aacctactat
     3781 gataaaaagt atcttgatta tttgaaaaaa ataaagagct ttatcgaaga tcaagattta
     3841 ttagatgatg aattttactc gattgtaaat gatcctatgg gcgcaattat ggaagtggac
     3901 ttttactacc agataccaaa aagctataaa aaagtctata acattatgaa gacaacagcg
     3961 ccagatttag ataatttagt aaaaagtgct atggatggta tttttaacat ctcagctata
     4021 cgagatagcc atatcacagg cttaattgct tttaaatata atgtggcgaa cgaaggaaaa
     4081 acagttgtga ggatcaaacg atattcagaa tttgagtggg atacttctga aggattgaac
     4141 ggagatatat gggaagctac atttgatttt aaaccagtag caacgccaag accaaagtct
     4201 aaatttagag gaaatgggat tatcacttac tatccaaaag agtacaacga ctatttggaa
     4261 aatatcaaga atacgcttaa agagaaagat ttggtaaatg atcaactgaa aaaggtaatg
     4321 aatgctccta tgggggttat agctcagata gattattttt accaagtgag aaaagataag
     4381 aaaaagcttg attcactgat gcgtacttca cagccggata ttgataattt agtcaaagga
     4441 acattggacg gaatttttaa caaggaaata ggcatcaaag atagcagggt tgtagggtta
     4501 attgctttta aatacaatac ttttgaaaag tcgaaaacaa acgtgagact acaggagatg
     4561 ggctgatgaa taataatgaa aaaattcttc atgtacttga tagctttgaa ataattcaag
     4621 aagaactaaa aaaatatcgg gatgttttgg aacaaagata tgattttgtc aatcagcaaa
     4681 agagtaatca catggatttc attctcaata tgaatgactt gaagaagaaa ttagttgaac
     4741 gaaaagaaca agaaaagttg atcaaagctt actttgaact cggtgaaaaa gaagttaaaa
     4801 atgcaatgga actgaatgaa gagcgcaggg ttttagatca gctgttagaa caattattgg
     4861 tgatgtttca aaaaggacga atagatgagg atttaattga agaaggattg agaaaatatc
     4921 cttcaaactc aggtatcgga atagtgctga aagcgattga tgaagaagaa attgaagctt
     4981 tcataccgcc agaggatttt gaatcggcaa tggaatatat aaaatattat agccaaggga
     5041 tcactgcttt tagagagttt gatcctgaag atgttattca tgacttgaac aacctaaaag
     5101 aatggtgtga aagttatgga gtcgatgatt ccggattgga ctacttgatc agtattatgg
     5161 aaatcgaaga agagatgccg gataagcctg atccaacaga tattttagaa ttgatccatg
     5221 aagctagaaa tccaattgcc tatatttcta gaggatatac tgtattggaa tactataagc
     5281 cttatatatc agcgatgaac catttaagac gtgtattaag ggaaaaacgt gaataccgct
     5341 cagtgttgaa tgcaacgaat agattagaaa aagctgtttc agagctagat gcttattatc
     5401 gtgaacatta cctgcaagca ggtggtatgc caagaaatac gaaagcaaat atcagtaggt
     5461 atataaaaaa agctgagtag gcggagttag cccataaaat ggcaaaaggt aacctgaaag
     5521 gggcattttg aatgaaaagg gatgtttttg aattgtacgt attagaacgt ctagaagacg
     5581 gcatgatggc acaagagata attgatgaaa ttgatgatat gatcagcaga tttggtcaat
     5641 tggagttgaa tgatggaaac aaaaattcgg tagtgctaaa acaattttgg ttgaacaatt
     5701 tttcgtataa ggattttatc aaagaaaagg caaatacatt gtactgacta aattcacgag
     5761 gagcagtgat gagagcttat tgctaagagc agttaggctc ttttttgttt gtgtaaaatt
     5821 atttgtttta taatacacat ttatacacaa aaaatattga cagatagtac acaaatgtgg
     5881 taggatatat ttgtaagcaa gttagattaa caagttaaag gaagagcagt tatgaaaaaa
     5941 gaagaactaa tagatatatt tcaaattgtt gaacgagcaa ataatatggg aattatgttt
     6001 tttgatcgta tttctttaaa
//
LOCUS       DACGLW010000117         5922 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.123, whole genome shotgun sequence.
ACCESSION   DACGLW010000117 DACGLW010000000
VERSION     DACGLW010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5922)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5922)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5922
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.123"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            complement(1126..1524)
                     /locus_tag="IXT53_002683"
     CDS             complement(1126..1524)
                     /locus_tag="IXT53_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906450.1"
                     /translation="MRLRRNKNYTTIILEDFTDQELLIVGKTKADFIEKGNASIVKAS
                     KIAVEKYFDSLEESAKEIFNGEKAGSYELDGIRFSLIPTTENIIPKKLYYIFSSEKHT
                     SMKPCIFDWQWLIDAVLDVKCSKYNITLGC"
     gene            1740..2795
                     /locus_tag="IXT53_002684"
     CDS             1740..2795
                     /locus_tag="IXT53_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParM/StbA family protein"
                     /protein_id="HAQ4906451.1"
                     /translation="MKEPLLFSVDLGNKSVKEMSTHVSPLSLPSRILNKNIINSGRFS
                     NIGSSLKRSLNVSEYSLVNTTESYYFGKDIHELGKDDYVIESLGFGISRYKQETYQNI
                     LSFSLANLARLEDQSDDVLTANLVLGLPSSDYTETIVKYVMQQATRQHSVEVDGKAYN
                     INVDTVWVLPQPIGTLYNLLLADDGSVQDETLISQKIGFVDIGGGTKLFDVVQNFEID
                     ENNREQKDTGIFTLYNRIVNMLDLPISPNAFQLEKIIREGKEKGVYLFKPSGNESFDI
                     SEVVEREIKTYTNMVVAEVLATFKNFTTLDKVVFTGGGASIIDKQLVKDKIPHTLFVE
                     DSEFSNVKGFYKFGLTQ"
     gene            2815..3168
                     /locus_tag="IXT53_002685"
     CDS             2815..3168
                     /locus_tag="IXT53_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906452.1"
                     /translation="MNKGKSERYVVIINREKVSELPLAEFLESEYETLGVKYSQTIKK
                     ALNLYRRVKNNEVTIIEAGNDRNDSKVAEVKNKPTDHNPIQQAHPSTVTSDHSAKKEK
                     PMLKGFNAPSNKDLL"
     gene            complement(3350..3646)
                     /locus_tag="IXT53_002686"
     CDS             complement(3350..3646)
                     /locus_tag="IXT53_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906453.1"
                     /translation="MIEVKENRFKKFAEAEVEEVPEVVEAVEEPKQTPKETTSSLVAK
                     LKSDSSEKKKNYQFTLQPSQRKKLSKISEKLGIRSDSALLGEMIDNLYDEVFGE"
     gene            complement(3650..4468)
                     /locus_tag="IXT53_002687"
     CDS             complement(3650..4468)
                     /locus_tag="IXT53_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ4906454.1"
                     /translation="MEKKLSIITGNIEKGGTGKTTTIYNLGGYAVENLGMKVLYIDED
                     KSQNLSKRFPDAFSKAKRENTINHLYLHGSAEPLAINENMDILVAGEDLKQVEMQMRD
                     MPNNRTLLFRWIVVNYEQLKEKYDLILIDTHNDAGLLAQNAWAVSDLVVGISDPSKDG
                     FEALLKLGKDIDALAKQLIDIKTGETLMETKYATIGSRIKHNTNSSREFLELVPTLSN
                     YLGYVQEKELLNATSLEGKNIAEFAKDKNIYRKNKDFFESTFTLYNKIIDTAQE"
     gene            4694..4915
                     /locus_tag="IXT53_002688"
     CDS             4694..4915
                     /locus_tag="IXT53_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HAQ4906455.1"
                     /translation="MPVISIRVSKEEKDFLEKVAEFNGDNISEFMRSNSLQTAETLVD
                     FATYKQLMKEHEENDQSISHEDMLKELGL"
     gene            4917..5183
                     /locus_tag="IXT53_002689"
     CDS             4917..5183
                     /locus_tag="IXT53_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ4906456.1"
                     /translation="MTYQVRYEREAQKALKKMDKFQAKVILNWIEKNLVGTDDPRKHG
                     KGLTANKSGYWRYRVGAYRIIADISEHEVTILILSIGHRSEVYK"
     gene            5296..5835
                     /locus_tag="IXT53_002690"
     CDS             5296..5835
                     /locus_tag="IXT53_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906457.1"
                     /translation="MYNISVNKLINFQKERFFMGWYGDYSSANEVIEEKKGYAKNGLE
                     IVGTKVTKTYGIILFKDAEGTYDIDSFIFRDEMYKPIYFLNDSPAYFKKIPKKWLKFF
                     VGDQEKMVKLQLDRIKEESNKPKLDDLLVAGKTYSIWDGSYVAVYQYKLKRSFIFRLQ
                     DGSLTRFTRLSAGDVKEVT"
BASE COUNT         2080 a          984 c          879 g         1979 t
ORIGIN      
        1 acattcctac gcacctcgag tgtaaaattt ctttattcct cccacgctcc atgttatttt
       61 atatttcctt tttcatgtta tttttatcca gaaaccgatc aagtttttac ctattcccaa
      121 aatccattta ttcgttattt ttttcactga ttaagaataa ttatttgatg cttaaattag
      181 ctaaaaaagc gatttgttac tgatataatc atgatattgc agctgaatta ggcggaaaga
      241 taaaatgttc gcaaaatcaa agtatcaatt atcttgtttt tttataaatt tgtaagtaaa
      301 aatctaaaaa agttttcttt taattggtat atgtattaaa atcacattta atcacccata
      361 aagtatgcgc tttcattttt ttggatgagt ttaatatcaa taaataacga tgtaaaaatc
      421 atttattatg ctcttttata aggatattaa ttgatctaat ttgaaaaaaa tcaaaattta
      481 aatgttttaa tattacaaat aaaatcatgt tattgtaaaa tggtataaaa aagctaaata
      541 ttctattttt gttattttat tagcaggaaa atttaaatag ttttacaaga atccgtagaa
      601 atatattggt tttgccttca gattaatact ggatcatatt aatctgaata taatatatga
      661 actaataaat agcttaacct cgctattttg ttttttctga tcactatcta tacagcatta
      721 gtaaatagaa ttttaaaaat aaaaaaatta agacaaaaaa gtatcaacat aaacatatac
      781 atatacacgt ttatgttgat tcacttaatt tgtcctttaa aaagcactta ttttaatata
      841 atgttatttt ataatcttat tacaagctga ttgttaggcg ttgtcctttc attacattta
      901 ttaacggtta ttttattgtt taatttatta attatttttg atctaaaact tagcttaaca
      961 aataaataat taaccaaccg ccagataaaa atgcgatcat tgctaataca taagcaaaga
     1021 taaaaatatg ttcgatcagc ttaaaccctg atgcgattaa gcagaccaag taaaatagaa
     1081 attctcttac agataatggt aattttctaa ctagcatact cataattaac accccaaagt
     1141 aatattgtat tttgaacatt ttacatccaa tacagcatca atcaaccatt gccaatcaaa
     1201 aatacaaggc ttcattgaag tatgtttttc agatgaaaag atataatata atttcttcgg
     1261 aataatattt tcagttgttg ggatcaagct aaatctaata ccgtctaact catagctacc
     1321 agctttctct ccattaaaaa tttccttagc actttcttct aaagaatcaa aatatttttc
     1381 gacagcaatc tttgaagctt ttactattga agcatttccc ttttcgataa aatctgcttt
     1441 tgttttaccg acaataagta attcttgatc agtaaaatct tctaaaataa tggtagtgta
     1501 attcttattt cttctcaatc gcatcaggat cactccttaa tataatttat gtttttataa
     1561 tatcataata agataaattt gcaagcattt atttacacct ttttatttac actttattat
     1621 aattaattag acttttatga ttgcattttt ttcttataaa cagttgcaac taatagtaaa
     1681 taggtgtaaa ctattttcgt accgaaatac cataaacaca aacagaaaga ggtcatacca
     1741 tgaaagaacc attattgttt tccgttgact tagggaataa atcagtaaaa gaaatgagta
     1801 cacacgtttc tccactctca cttccctctc gtattttgaa caaaaatatc attaattcag
     1861 gacgtttttc taatataggt tcctcattaa aacgatcact aaacgtttct gaatatagcc
     1921 tagtaaacac tactgaaagc tattactttg ggaaggatat acacgagcta ggaaaagatg
     1981 actacgtgat agaatcacta ggttttggga tcagcagata caagcaggaa acctaccaaa
     2041 atatcctatc tttctcttta gctaacctag cacgtttaga agatcaatct gatgacgttt
     2101 taaccgctaa tctagtttta ggtctgcctt catccgacta taccgaaaca atcgtcaaat
     2161 acgtgatgca acaagccaca cgacaacatt cagttgaagt tgacggtaaa gcttataata
     2221 taaacgtaga taccgtttgg gtactgccac agccgattgg aacactatat aacctcctac
     2281 ttgctgatga tggtagtgta caagatgaaa cattgatcag tcagaagatc ggttttgttg
     2341 atattggtgg tggcacgaaa ctttttgatg ttgtccaaaa ttttgaaatt gacgaaaaca
     2401 accgtgaaca aaaggatacc ggtattttta ctttatataa tcgtatcgta aatatgcttg
     2461 accttccgat cagtcctaat gctttccaac ttgaaaagat tatccgagaa ggcaaagaaa
     2521 aaggcgtata tctatttaaa ccaagcggaa atgaaagttt tgatatttca gaagtagttg
     2581 aaagagaaat aaaaacatac accaatatgg ttgttgcaga agttttagct acattcaaaa
     2641 acttcacaac cttagataaa gttgtcttta ctggtggtgg ggcttctatt atcgacaaac
     2701 aacttgtaaa agataaaatt ccgcatacct tgtttgttga agacagtgaa ttttctaatg
     2761 taaaaggctt ttacaagttt ggtttaactc aataaaacag cgaaggagat caacatgaat
     2821 aagggaaaat cagagcgtta tgtagttata atcaatagag aaaaggttag cgagttacct
     2881 ttagcagagt ttcttgaatc tgaatatgaa acactaggcg ttaaatactc tcagacgatc
     2941 aaaaaagctt tgaatctata tcggcgtgta aagaacaatg aagttacaat tattgaagca
     3001 ggcaacgata gaaacgatag caaagtagct gaagtaaaga ataaaccaac tgatcataac
     3061 cctattcaac aagcgcatcc ttcaacggtt acatccgatc attcagcaaa gaaagaaaag
     3121 ccaatgctaa aagggttcaa tgcaccaagt aacaaagact tactttaaaa ataaaaaggc
     3181 gaggttacaa tacctagcct ttttattatg tacaaataca aacataaaca tatacgtata
     3241 catgtatata aacatgtata cgtatatatc cataaacacg tatacgaaat ttagttaagc
     3301 tatacctaac cgcttattcc aataaaaaaa gtgatcagct ttaaactgat cactcaccaa
     3361 aaacttcatc atacaagtta tctatcattt caccaagtaa agctgaatct gatcgaattc
     3421 ctaacttttc gctgatttta gatagtttct ttctttgtga tggttgaagc gtgaattgat
     3481 aatttttctt cttttcagaa ctatcacttt ttagtttagc taccaaagaa cttgttgttt
     3541 ctttaggtgt ttgtttaggt tcttcaactg cttcaaccac ttcaggaact tcttctactt
     3601 cagcttcagc gaatttttta aatctatttt ctttcacttc aatcactcct tattcttgtg
     3661 ccgtatcaat tattttgttg tatagcgtaa acgtgctttc aaagaaatcc ttattcttac
     3721 gatagatatt cttatcctta gcgaactcag caatattctt accttctaag ctagttgcat
     3781 taagcaattc cttctcttgt acatatccaa gataattaga taacgttggt accaattcta
     3841 ggaactcacg acttgaatta gtattatgtt tgatcctgct accgatcgta gcgtatttag
     3901 tttccatcag tgtttcacca gtcttaatat caatcaactg ttttgctagt gcatcaatat
     3961 ctttaccaag cttaagcaaa gcttcaaaac catctttaga tggatcagaa atgcctacga
     4021 caagatcaga tacagcccaa gcattttgag ctagtagacc tgcatcatta tgagtatcaa
     4081 tcagaattag atcatacttc tctttaagct gttcatagtt aacaacgatc catctaaaca
     4141 gtagggttct gttgttaggc atatcacgca tttgcatctc aacttgtttt aaatcttcac
     4201 ctgctactaa aatatccatg ttctcattta tagctaaagg ttcagctgat ccatgaagat
     4261 ataaatgatt gattgtattc tctcttttcg ctttactgaa agcatcagga aatcgttttg
     4321 ataggttttg cgacttatct tcatcaatat acagcacctt catacctaaa ttttcgacag
     4381 catatccacc tagattgtag attgttgttg ttttacctgt tccacctttt tcaatgtttc
     4441 cagtaatgat tgacaacttc ttttccatga ttaaacctcc cttattaatc ttttgtaact
     4501 ccattataca ctataaacac gtttatgtat atatttatat acataaacgt gtttatattt
     4561 atatttgctt ttatattcta caacataaac atatatatgt atatgtttat gtttatgtat
     4621 atattcaaat atataactat tgcatttgtc atacaatcgt agtacaatgt aattgaaaag
     4681 gagttgataa actatgccag taataagtat tcgtgtatct aaagaagaaa aagattttct
     4741 tgaaaaagtt gctgaattca acggagataa catttctgaa tttatgcgta gtaattccct
     4801 gcaaacagca gaaacgttag tagactttgc cacctacaag caactaatga aagagcatga
     4861 ggaaaacgat caaagtattt cccacgaaga tatgctaaaa gagttaggct tataagatga
     4921 cttaccaagt aagatatgaa cgtgaagctc aaaaagcgct aaaaaaaatg gataagtttc
     4981 aggcaaaggt tatcctaaac tggatcgaga aaaatcttgt aggcactgat gatccaagaa
     5041 agcatggtaa aggtctaaca gctaataaat caggctactg gcgatatagg gtaggtgcat
     5101 accgtataat agctgatatt agcgagcatg aagtgacaat tcttatattg tctattggtc
     5161 acagaagtga agtttataag taacagacaa gctgataagg cttgtctgtt tttttgttaa
     5221 cccaacaaaa tcactgttta cgatcccact ttacctaaaa aagaacaata tcctcttgat
     5281 attatacaca ccagtgtgta taatataagt gtaaacaagc taattaactt tcaaaaggag
     5341 cgatttttta tgggatggta tggtgattat tcatcagcaa atgaagtgat cgaagaaaag
     5401 aaagggtacg ccaaaaatgg tttggaaatc gtaggtacca aagtaacgaa gacctatgga
     5461 attatccttt tcaaagatgc agaaggaacc tacgatattg acagcttcat ctttcgagat
     5521 gaaatgtaca aaccaatcta cttcttaaat gacagtcctg cttactttaa gaaaattccg
     5581 aaaaaatggt tgaaattctt tgtcggtgat caagaaaaaa tggttaagct acaactagat
     5641 agaattaaag aagaaagcaa taaaccaaaa cttgatgact tattagttgc aggtaaaact
     5701 tactcaatct gggatggatc gtatgtcgct gtctatcagt ataaactcaa acgtagcttt
     5761 atttttcgac tacaagatgg atcactcact cggttcactc gactatcagc aggagatgtt
     5821 aaggaagtaa cctaaaactt aaatacataa acataaacgt gtatacgttt atgggttctg
     5881 ttgcaaagtt ttaaatctac tatcaaataa ggtagaataa ta
//
LOCUS       DACGLW010000118         5888 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.124, whole genome shotgun sequence.
ACCESSION   DACGLW010000118 DACGLW010000000
VERSION     DACGLW010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5888
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.124"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            complement(74..158)
                     /gene="ffs"
                     /locus_tag="IXT53_002691"
     ncRNA           complement(74..158)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="IXT53_002691"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(181..696)
                     /gene="tadA"
                     /locus_tag="IXT53_002692"
     CDS             complement(181..696)
                     /gene="tadA"
                     /locus_tag="IXT53_002692"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HAQ4906458.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            803..1660
                     /locus_tag="IXT53_002693"
     CDS             803..1660
                     /locus_tag="IXT53_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fis family transcriptional regulator"
                     /protein_id="HAQ4906459.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(1741..2076)
                     /locus_tag="IXT53_002694"
     CDS             complement(1741..2076)
                     /locus_tag="IXT53_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906460.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(2143..3513)
                     /locus_tag="IXT53_002695"
     CDS             complement(2143..3513)
                     /locus_tag="IXT53_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NOL1/NOP2/sun family putative RNA methylase"
                     /protein_id="HAQ4906461.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(3591..4127)
                     /locus_tag="IXT53_002696"
     CDS             complement(3591..4127)
                     /locus_tag="IXT53_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HAQ4906462.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            4436..>5888
                     /locus_tag="IXT53_002697"
     CDS             4436..>5888
                     /locus_tag="IXT53_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ4906463.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKK"
BASE COUNT         1867 a         1202 c          874 g         1945 t
ORIGIN      
        1 aaaatcagga acattcattt ttacagtact ataaaaataa aaaaaacaga cccgaaagtc
       61 tgttgtcgtt tcattataat cagacacatg gcggaacctg cttagtgctg cttccttcca
      121 gacctgacac gcttcacaag cccaccattg tcctagcctt tcggcaaaga atagtatacc
      181 ttatttttca ttcgttgacc agtctttttt taatttcttt tttcgctggc ggagctttcg
      241 gaaaaagtca gtcaaaatca tcccacattc gtcttctaaa atgccacctt ctacatatgc
      301 ttggtggtta aaccgctcgt cctccaaaag attcatcaag gtaccggctg ttccgccttt
      361 tggatcatac gcgccgaaat aaacttctgc tacacgagac aaaatcatcg cgccgctgca
      421 cataggacaa ggctctaagg taacaaaaag tgcagcatct tccaagcgcc agcttcccac
      481 tttttcacag gcttccttga ttgcaagcat ttctgcatgt gtagtagcat cttgcgtggt
      541 ttctctaagg ttatatcctc ttccaatcac ttttccatcc aatacaacca ctgctccaat
      601 cggaacttca tgaagcattt ctgctttctt tgcttctgcc aatgcaaggc gcatccattt
      661 ttctttttct actgcatcaa gatttgtatt taccaattac ccttccctgc ctttgttttt
      721 tcttgtttta ctttagcata tgataaaaag gctgtaacta actttttttt ctatggtaaa
      781 ataaatacaa gaggtgaaca agatgaaaat ggataaatta cttgctcttt acccacaagc
      841 acagaaaaaa aactctccag catcagaagc ggatatcctt tcttttgcaa ttgatgatgc
      901 ttttatctgg atcaaacaag attcattatc tcaacaagaa accagccttt tgaaagcgtt
      961 attccctgta atcaacgatc aaaaaaaaca cccatggtat cactatcttt ttaaagatgc
     1021 ttcttgtgtt caagaaaaaa gctttcgttt gatccagctt cacgtcgaat caaaaggaga
     1081 attcttaaaa aaagaatggc aagatactat tttggaatta tttcgctatt ctgctgattt
     1141 ctttttttat acagacacag atgcgctctt agtcgaacaa ataaaccgtg attatcttga
     1201 tgctactgaa atcaacggta tttttctatc tttagatgct gattttgata cgacaacgtc
     1261 tgtattcgtt ggttctttcc attcacctgg caccgacctc gtccctttgt ttgctgaaga
     1321 aagaaaaatt tttttaacag agcaaaacaa tctttatact cacagccgta ctttttctat
     1381 gcaagatgtg gctttacatt actatacaaa agatactgta aaagatagta agttgatcaa
     1441 aagttacata gaactgatca aacaaaatga aatggaagtg atcattcaag aattatggaa
     1501 tgaattaggc aatgtcagct ctgctgcaaa aacactgttt cttcatcgta atactctaaa
     1561 atatcgaatc gaaaaatttc aagaacagtc tggtttcaat ctaaaaaaag ccaacgattt
     1621 actgttttgc tatcttttat tgcttcatga acataattaa agatagatta aaggggttgt
     1681 gacaataatt ttgtcacaac cccttatttc gaatgaatag tgttcaaaag ctagtttctc
     1741 ttacatcatt ttttcaacaa aatgcattag ccggtctttt acatcttcgg ttgaatcttt
     1801 gattgaatca ccataaacag agattttctt ttttcctgat ttgatttttg ctaacattcc
     1861 catcaaagaa tccaagtcac tatctgaaag accatctacg atttctagta atttttcatt
     1921 tccatcaaat ttgtcataga cgaatttttt tactttcgtc cgagtagtca tatgatggat
     1981 cttttccatt acttttcccg atacaacgac tgctgtcgct acactggcag cggctgctac
     2041 gcttaatccg attgttactt ttgttgatgt tttcataagt atcagctcct ttttatctat
     2101 ctccatcata gcatagacaa aaaaataaac gaactaatcc actcagtgaa atctcaatcc
     2161 tttagggaaa aagttcttta ctgttccttt tacttgtttc ccaaatccga caggtatctt
     2221 atctaatact agcaaaaccc atccttgatt cccatcacgt tgaaatgttt ctcctgctgt
     2281 gtaagattgc cattcctttt gagtgatcgg caagcaaggt atattctcta tcttttttgt
     2341 agcaagcgct aatgcatagc taggttcaaa tcgattcttt ttgaaatctc ctaaatgcaa
     2401 acctgtacgt acgactttta aaccatcgag agaaggggct aactcaggaa cttcccaaag
     2461 atggtcgttg aacactaaaa gtcttccagt agcttcataa tgaaaatcat ttgaaaactc
     2521 cgtccagagt ttttcctgct cttttgtcat ttgcacttta cttttttttc gtgtcttctt
     2581 ttctttatgc atctgatttt gtccatgaaa agtcagcttt gcaacaaaat gtccttcacc
     2641 ctgatcttta tgaggccata tacgtattgt tttttccaaa ccagctacgc ttccccattc
     2701 agaacgtcct gaactaacgg attgagtcaa aggaatctcc tcaatcgtta ctggataatt
     2761 ttctacaagc cagctaatga tctcttcatt ttcttctggt gcaaacgtac aagtcgaata
     2821 aatcagttgt cctttatttt ttagcatttt tattgctgaa gacaaaatct cttgctgacg
     2881 tttttgacag tatagcggag attcttctgt ccactctttt attgcatttg gatcttttcg
     2941 gaacattcct tctcctgaac aaggagcatc aaccacgatc cgatcaaaaa agcccgaaaa
     3001 atgagggaca agttctgcgg gtgcatgatt tgtcactatt gcgttcgaaa ctccccatcg
     3061 ttctatattt tctgataaaa tcttcgcacg tttcggaaag atctcattcg tcactaataa
     3121 ccctttcccc ttcatttgag cagctaattg ggttgattta ccaccgggag ccgcacacaa
     3181 atccaaaact ttttcaccgg gttttgctgc agcagctgtt ccaacgatca ttgcacttgg
     3241 ttcttggcta tattcatacc cagcttgatg gaggaaagac tttccattga cagtgccaag
     3301 aaatccctca ttgctgtagg gagcgggttg taattcttcg ctatggtaag tttggaccat
     3361 gtccaatcct gccggtttta gtggattcca ccgaaatcct tttttcacgc ttccttgttc
     3421 aagcgcagaa aaaaaatcgg atgcttcttc gcctaaaaga agacgatatt ttttgataaa
     3481 ttgctgtggt aacgttgtcg cttcctcctt cattccaatg tttcgttaga cattttacaa
     3541 tcttttttac gaaaaataaa ggtggctgat ctactttttc atgttgctgc ctaaggtgac
     3601 tgttccttta agatattttc caagtattca tccgtcacct cgtttttatc ccaaacagcg
     3661 ttcatcattt caagaaaatc ttgagaagaa tcatctgagc gcaaacttcc caatgcatta
     3721 agccatacca attcaagcga cttttcttct ttgatcttcg tgtgaccttt gacatatgag
     3781 aaaaatggcc gcttaaatag cgaatccatg tctaaatcag attttgcaat gatttcacga
     3841 atactttcat ggatagaaaa aaaatctttc aataagtaga caatgcttaa aacatcatcg
     3901 gaactgcatt cagctaagta gttttcaaaa taaacagtga tttcttctaa tatcttctct
     3961 tttaacccgc tgattgattt gaaatgtgta taaatcgggt atgtagaaca accgcagaac
     4021 tctgctaact ctcttgcagt aattgacttc attccatatt cttccacgta taccatactt
     4081 ttgaacagca aatattcttt actataattt gttactctcc ccatcattct tacctccgtc
     4141 tcttttcatc tacccataaa taaataacat aaataattct aaaaatatta ttgataatta
     4201 tcaaaacata tttagttata tccattaaaa atcaattttt ccgccttaaa aacaagaata
     4261 tcttgacaaa ataccctagg aaataaacaa tagaataaac aaggtaacaa aaaaattaaa
     4321 taacgttata taaaaaagaa ggcgacactc tgtgagaata acacagactg aaatgacttt
     4381 ccactcacag tttttgtcta actatataca aaaacagaat aaaaaggagg tatttatgct
     4441 aattgaagca agaaaaaaag agttacttgt tctacaaatg gtcattcaaa aaaataattg
     4501 ttctttacaa gaattatcca atgacttaca tattccaaaa cggacaatca aagaactgat
     4561 ccgaaaaata aatgtatcac tcaagcaatt tttattgatt gatgaattta tttattctaa
     4621 tcataaagga gagatccaaa ccaaagaaac cgatcaaact aaaaagttag ctgttttttc
     4681 taagttaaaa ttatgctacc taaaagaatc caatcgtttt aactatttga tgttactaat
     4741 caattatcca caaactgctg taccaaaaaa atatttactt gaacaacttt atatatcgcc
     4801 ctcctatctc gaaaaactga caaaacagct caatcattcc ttaaaaaaat tccatttacg
     4861 gatcctatct gtaaaaaatt gctgtactct tgaaggaaat gagttatcta tccgtctcta
     4921 tctttacttt tttctttcag atacatttgg aggtaacgat tggccatttt cctcacttga
     4981 attaaatgca ctaaaaatga acagtacaga tgagcaaata gaaaaaattt caaaacaaga
     5041 aaaaaatatc tacttgctgt taactttatt catattacga aacaaacaac aggcatattt
     5101 tccaaaaact tcttctgaaa tcactgaatt gatggactta ttgcagcata cacatgatta
     5161 ttcagaatac tttacttctt tttcagtgag tcgcccttgt gaagagacag aaacagagag
     5221 agcctacttt aattattttt tctattgttt ttttccaaat caaatctctt ttcgacaaaa
     5281 acaggagatt ggttttttat tttccacaag caaaaacctt atttgtcgac aagtcggtca
     5341 attgatgact tacttaactc aacagtttcc tctcaaccat ttagaaaata aaataccaat
     5401 ttatttttat tttcttgtgt tggcagttgt ttttttcgaa ctgttgggtg aatccagcag
     5461 tagtttttct acactttgct ttccagaagc cacgtccaat cattctagtt cttttataca
     5521 ggaaaagaaa ttaagagaaa aaatcgatac aattttttca aaaaatgggg aaagaaacca
     5581 aatcagtcaa taccttagtc aattgatcca acacttacta caaacagaaa cgaaagagca
     5641 gttactcatc catttgcaga taaccaaaca gtttacagga aattatcatc ttcaaagcca
     5701 tttgcagcag ttatttcgca aagatgctgt taaaattacc gcatattcag aacatgctga
     5761 tttgatcatt acagatcagc atgagaaaat ttcagatgaa acggagactt tctatctgga
     5821 tacaacgcac aatacagtgt tatttggcaa gttaataaaa gcggttcatg aactgtatat
     5881 gaaaaaag
//
LOCUS       DACGLW010000119         5884 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.125, whole genome shotgun sequence.
ACCESSION   DACGLW010000119 DACGLW010000000
VERSION     DACGLW010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5884
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.125"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            complement(74..1168)
                     /locus_tag="IXT53_002698"
     CDS             complement(74..1168)
                     /locus_tag="IXT53_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ4906464.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1357..2118
                     /locus_tag="IXT53_002699"
     CDS             1357..2118
                     /locus_tag="IXT53_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ4906465.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2233..2673)
                     /gene="fabZ"
                     /locus_tag="IXT53_002700"
     CDS             complement(2233..2673)
                     /gene="fabZ"
                     /locus_tag="IXT53_002700"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HAQ4906466.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            2893..3648
                     /gene="fabI"
                     /locus_tag="IXT53_002701"
     CDS             2893..3648
                     /gene="fabI"
                     /locus_tag="IXT53_002701"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HAQ4906467.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(3758..4771)
                     /locus_tag="IXT53_002702"
     CDS             complement(3758..4771)
                     /locus_tag="IXT53_002702"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HAQ4906468.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            4936..5856
                     /locus_tag="IXT53_002703"
     CDS             4936..5856
                     /locus_tag="IXT53_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HAQ4906469.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT         1789 a         1174 c         1054 g         1867 t
ORIGIN      
        1 tataaaaaaa caaaataaaa aagcagaaat aactggctat ttctagcgga ttatttctgc
       61 aataactaat tatctatttt ttattttcaa ttaatttaaa gcttgaccat ggtttcaaaa
      121 agtcaaggag gaataattcg tcatcggaga ttcttcctac aacattgaca cgtccgtcgt
      181 ttttcatatc cttcaaggcg atttgtgttt ctcctttgta ttgaccgtac ccttcattgt
      241 caatcagtac atccccatga tgcatatctc gcgtattatg aggcgggaaa tctttatctt
      301 tgtagtatac acgagtcatc gttgaacgaa ggatatattc tgagcgatcc cctcgataac
      361 tatgaaggtt atcaaacaag cagattcgtt cgttttctgt aataccttct tccgtatcta
      421 ctttcaatgt tggataatcc gcgttaaatg cttctgccat ttctttcaat tcttcttcac
      481 ttgcgtacgc atttccaata gaaatatcat caatcaatcc tgtaaggatt aaatgcttca
      541 cttgtgtcgc aatttcaaga tctcggtggt cttccagcgt gcacaagcca tcttgcgtag
      601 gccaagggcc aaactcagca gattgcgaat tgacgaatgc catcgtattc aaattatatt
      661 ttctgaattt ttctgaacaa aaaacaaagt gttcgtatcc taaaccagag tatcgatgag
      721 gataaaaatt atgagaacct aataaattat ccgtgtttgg cgaataactc atgatgttgt
      781 caacgtaatt cgtgcccgaa ctcatattga tctcgatttt gataccaaaa ggattgcgcg
      841 tcatttttgc ttcttctgct cctgtgaatc cgatatctaa gcggaccccg taggcaccca
      901 tcttatggaa aaaagataag tcatcgtaag aaatctccaa ctgttcaaat aacgctggat
      961 tgatatcaac cattacttcc atgcctaggc tattcgcgta atcaactact tctttaaatt
     1021 ctgaaagaac tttttcttta tcatcattga tctgcagcaa actggtaaaa acccgtttat
     1081 acccatactt atgagctaaa tccaagtacg ctttgtcctt ttcaaatgtt gaccgttctg
     1141 gataaatcga gattcctaat tttcccatat taccatttcc tcctgcatct tttcgttttt
     1201 gtatccactt acattatagc aaacacaaag taaatggtct acaccttttc tttttctttt
     1261 aaaataaatg ataaattcca ataatttaag taggtatttt ccctctattt tgttatcatt
     1321 aaagataaca aaatagtaaa ttggaaagga acagttatga agaaaaataa agttatttta
     1381 agtattagtt tagcagcagc attgctgttt gctgctggtt gttctgcaaa caacacgact
     1441 tcttctagtt cgtctacaga aagttcaaaa agtacatcta ctgcggaaag cacgactgcc
     1501 agtagcgtcg atttgaacgc attggaatta ccacaattga cgaaagatgt cgccgaaaat
     1561 gaagatctgg tcgaaatggt gacgaataaa ggcacaatca aaatcaaatt atttcccgaa
     1621 cacgcaccaa aagcggttga aaactttatg acacatgcaa aagacggtta ctacaacgga
     1681 ctgactttcc atcgcgtcat caaagatttc atgatccaag gtggcgatcc taaaggcgat
     1741 ggcactggag gggaaagcat ttggggagaa ggatttgaaa cagagatcaa caaccacctt
     1801 tataatataa gaggtgcgtt atctatggct cgctcacaag atcctaacag taacggcagc
     1861 cagttcttta tcgttcaaaa tacagatgat atgcatgatg gtcttttaaa agacgactat
     1921 cctcaagcaa tcatcgacgc ttataaaaac ggcggttatc cttcactaga tggccattat
     1981 actgtcttcg gccaagtgat tgaaggaatg gatgtcgtgg atgctattgc aaatatcgaa
     2041 acagatagtt cagacaaacc aaaagaagaa gtaaaaatcg ataaaatcaa tatactgcaa
     2101 gaagcaaaac aaaaataaaa aaataacgtc tgaatcctgc atagatgaca acgtatgcta
     2161 ggattcaggc gtcttctttt tacataaaaa gcaagtaagc ttccttttgt tttgctatat
     2221 caggttttat agtcaagcat tttggttgac gatgaacgtg aactcgcact cacagacttt
     2281 tttatcatct acatatgctg cagataacgc tgtaccgact gatcccttca ttttggtgat
     2341 ttcaaaatga catttcagca catctcctgg aacaactttt tgacggaatt tggccttatt
     2401 gatcccaccg atataagctc tttttcctag aaactcttct gattttagga taagtatcga
     2461 acctgcttgt gccagtgttt ctagaatcaa cgcacccggc atcactggat tgtcaggaaa
     2521 gtgaccttgg aaaaaatctt cattgattgt tacatttttt gtcgcaacga tcttttttcc
     2581 tggttctaaa gtatctacat aatcaatata acaaatagga taacgattag gaatcaattc
     2641 cattacttca gtagcagata atacgtaaga cataattaaa ctccttcttt caggcaattg
     2701 ccattttcat tactttgact atcaaagtat catattccta aatatttagc aatctttttt
     2761 taatttaaga aaaaatagtt tgacaatcaa aacattttga ctaataatta agaaatcaag
     2821 aagttatttt agctattatt ctctattttt ttacgactat caaattttta tcagaaacaa
     2881 aggagagtca ttatgtcatt tctagaagga aaaaaaatcg tcattatggg cgtcgctaac
     2941 aaaaaaagca ttgcttgggg ttgtgctgaa gcaatgttga aacaaggggc agaaattatc
     3001 tatacttacc aaaatgaacg aatgaaaaaa tcattagtca aattggtcgg cgaagaagca
     3061 ttcactgtag aatgtgatgt ttctagcgac gaaagcatcg aaaaagcaat gaatgccatc
     3121 ggtgaatatg ctggggagat ccacggcctt gtccatgcag ttgcttacgc caacaaagaa
     3181 gaattgtccg gaaatgtgtc tgatatttcc agagacggct atcaattagc tcaagatatc
     3241 agcagctact ctttaattgc tgtatcaaaa tacgcacaac cgtttcttgc accaaacagc
     3301 gggatcgtta gcatgactta tctaggttca gaacgcgcaa tcccgaatta taacatgatg
     3361 ggtattgcga aagcttctct tgaagctgct atccgttatt tggcagctga gttctcgcct
     3421 aaaggaattc gggtcaatgg aatctcagct ggggcaatca aaacattagc tgtcacaggt
     3481 gttaaagact atcaaaaact gatccagtta tctgaatctc gtacgcctga tcagcaaggt
     3541 gtgacgatcg aagaagtcgg taatgcttgt gctttcttaa tcagcccact ttccagcgga
     3601 atcatcggtg atattatcta cgttgacaaa ggtgtccatc tttcataaac ttttgttgca
     3661 ttctttaaag taaaacatat aaaaatcagg aaaaaacttt ttaaagtctc ttcctgattt
     3721 tttcttttta cgtttttttc aactcattac tttgaaatta ttccacgact tcaatccagc
     3781 cttctggtgc ttctatgtct ccaaattgaa tacctgtcaa ttcatcatac aaccgttttg
     3841 ttactggtcc tacttccgtt tcactataaa atacatgaag attgttattg tgataaatcc
     3901 caccgattgg agaaataaca gccgctgtac cacatgcacc agcttctgca aattgatcta
     3961 gatcatcaat gaaaatatct ccttcaattg cttgcagtcc tagtcgttct tcagctaact
     4021 gcaacaagga atatttcgtg atacttggta agatagaggg cgattgcgga gtgatgaatt
     4081 ttccgtcttt tgtaattcca aagaaatttg ctgcacctac ttcttcgatt ttcgtatgtg
     4141 tggctggatc aagataaaca caatctgagt aatccagttc ttttgcttct gttcctggaa
     4201 gtaaactcgc tgcgtaattt ccgccgactt ttgctgcacc agtcccctta ggagcagcac
     4261 gatcgtaatc agatacaata aaattcgttg gttttagtcc acctttgaaa tacgctccta
     4321 ctggcgtaca gaagacgaca aataaatatt ctggagctgg atggacacca atgttttcac
     4381 cgaccccaat cagtaaaggg cgcaagtata atgtagcgcc tgttccatag ggcggcacaa
     4441 aatcttcatt tgcttttacc acttgcttga cagcatcaac gaacatcttt tcagggacca
     4501 ttggcatcag caatcgtcta gcactttgat tcatccgtcg actattttgt tcaggtcgaa
     4561 agagattgac cttgccgtct tttcgacgat aagcttttag accttcaaaa cattgctgac
     4621 cataatgaag cgctggagag ccttcatgaa tcgtcaggta attatcttct gtcaattctc
     4681 cttcttccca ttgtccgtct ttccatttgg caataaaacg ccaaggagtt ttgatataag
     4741 aaaaacctag attgttccaa tcgatttgca tatacttacc accttcgtta ttttctccac
     4801 attttatcat tatccatatt atttgtcatt ttattttcag cttttttcct tcattatagt
     4861 tatttttgct atttcctttt ttgaaaactt cttggtacaa tacagaaaga atcaaatttt
     4921 agaaaagagg attttatgtt ttcattatta gcaactgccg gtacgacaga ctcttctggc
     4981 gagacattga cagatcaagc tgtcagcaaa gtaaatgcgt ttcagcggtt ttgggacggg
     5041 attgattggg acgccatttt ggctgccttt atccaaaaaa gtttatatct gatttttttg
     5101 atcgtccttt tttggattct tcaccgtgtg ggaaaatatc tgattgataa gtcttttaat
     5161 cagtattcta aaaaagccat tctaaacgag agccgcttaa agactttaca tagtctttta
     5221 aatactattt ttcattatac ccttggtttc ttttttatct atgcaatttt atctgctatc
     5281 ggtgtgccga tcggatcact tttggctggt gccggaattg caggtgtggc aatcggtctt
     5341 ggtgcccaag ggttcatgag tgatgttatt accggatttt ttattatcat ggaacaacag
     5401 atggatgttg gggactacat caagctagca aacttaacga ttgaaggaac cgtcgtatct
     5461 gttggtatcc gtacccttca gctgaaagca acggatggta ctgtccattt catcccgaat
     5521 cgaaacatca ccacgatcag caatctctct cgtgcaaata tgcaagtact tttagatatt
     5581 cggattgttc ctgaagaagg atatgacaaa atctatgaga tcattgatcg agtaaatcgt
     5641 acattagctg aaaaatacca agaagagctc caaactgaac cttctatctt tggattagtg
     5701 gatcttggaa acagcaattt tgcgattcgt acggtctgct acgcattgaa cggaaaacaa
     5761 tatgccttaa aagaagagtt cttgagctcc tatgtcaaag aattgacagc ttctggtttc
     5821 acgattccga acagtccaat tgctgtcgga aaataatttg gacaaacgat gcaacgaatc
     5881 tcac
//
LOCUS       DACGLW010000120         5769 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.128, whole genome shotgun sequence.
ACCESSION   DACGLW010000120 DACGLW010000000
VERSION     DACGLW010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5769
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.128"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            complement(1014..1304)
                     /locus_tag="IXT53_002704"
     CDS             complement(1014..1304)
                     /locus_tag="IXT53_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906470.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            1646..1942
                     /locus_tag="IXT53_002705"
     CDS             1646..1942
                     /locus_tag="IXT53_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906471.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1949..3901
                     /locus_tag="IXT53_002706"
     CDS             1949..3901
                     /locus_tag="IXT53_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAQ4906472.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3973..4470)
                     /gene="dfrG"
                     /locus_tag="IXT53_002707"
     CDS             complement(3973..4470)
                     /gene="dfrG"
                     /locus_tag="IXT53_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAQ4906473.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(4797..5489)
                     /locus_tag="IXT53_002708"
     CDS             complement(4797..5489)
                     /locus_tag="IXT53_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAQ4906474.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT         1894 a          996 c          917 g         1962 t
ORIGIN      
        1 atacttgcaa agtaatacta caattcctaa cataatcgta gaatttaata atttccatag
       61 cataaagtgt ttagaaaaga acattaagaa tagttctata ataattcttg aactccatgt
      121 cttataatgt tctatcgaag cacccaaata gccaagttca tctaatctac ttgcataaac
      181 tacgtcatcc gctctcatag tcggcatata catattaaag agtaaaaaga taccccaaat
      241 ccctattagt aacaagatca gttctttgtt attcgttatt tttctattat gtttgttcat
      301 ttttatactc cttcttatat ttcctaatag agtataacat agctttaagc attacttaaa
      361 gataacttaa aagttatctt ttaaatactt aattgttaaa taccttattt gatgcaaaag
      421 aaatctacga tagtcaacaa aaaaattttc attatatcca gaaaaatttt tcttagttaa
      481 ggacctatgg attgcagtaa tgcagaattc gtcgaataaa ctgccaaaga catacaatca
      541 tattgctttt aaaattgacg aacaagatat agattcgttt gtctcaaaaa tccaaatgtt
      601 aggtttaact gtcgaaccag gacgttctcg tgtgaaaggc gaggcttctt caatttactt
      661 ttacgactat gataatcacc tcttcgagtt acataccggt actctccagg aacgtttaaa
      721 atcctacaat tccactacct gagactagta atgactagtc tctttttatg gcctctgatc
      781 cccccattga gcgaatacta atagggcttc gaattgatct gttcaatctt taagcaatat
      841 ccctcaaatg agtttggaaa gtttagtgat tcatcacaaa tacttaaata catgtagtta
      901 tatatagatt gaggttcttg tgagcgtatg ctcgtgtggg ttcaaatccc atcgtctgca
      961 tccatctttc caaaacaagc ggctaggaca tttttgtctc agccgcttgt tttctagttt
     1021 attctttcat cttgtcggac cttttttctt gggtatagac ttttctcata ttctaccaca
     1081 attttgggtt tccctaacac atgataggat ctcacaacaa attgctttct attattataa
     1141 taacctgtaa cgactaactt catcccttcg tctacttcag ctaggaagtt caaactatgg
     1201 gaagcaatca aacaattgac cccatttaaa ctgaaacgga tcaacggtct ggcactaact
     1261 tgcaaagtac ggatcttatc tactgttcca tattttttct ccataacctt cacctcaata
     1321 ccattatacg aacatacatt ccctttgtaa agaagaaact ctgttttatc tttttgactt
     1381 tttgtctatt acctttctta aaatcatatt ggcacttcct gcgaagatga aggacatgta
     1441 ctaaaaaaaa gaggcagcag tatagattta agtagttcag ttttggagta caaaaagtga
     1501 aaatgtaaac ctaaatgagt atcaaattgc aactgaattt gatactcgtt ttttattgtt
     1561 aaatcaacgt ttgttgttaa tttacaccac aaaatgtaag ttaaaatgta tataaaaatg
     1621 cgaacgtgaa ataattattg taggaatgca aattggtgtg ttaaaatggt tacaatttac
     1681 taagcaaaaa ggggtgcatt ttcctatgca atttcttgag ccaaaaaata agaacgctaa
     1741 gtctgtggat tgggaaatat cagagcaagt aagggtgata gttaagcaat acgctgaata
     1801 tgcagagcgt actgaaagcg aagcagtgga tgaattctta ttgaacattt tagacgataa
     1861 gaagtttatt gaatggattg ccaacaagcg aagtaataag cggattgttg aaaaaatggg
     1921 tatcaaagat agagtgggtt gatagacaat gaccaagctt aaaagattag ccacgaagga
     1981 agatgaaatt gtagatgtta aaattccgat ttcaaacgaa gaattaaaag atagggctaa
     2041 acaatatgat ttattaactc caaaacgatt tgcaacaagg tataacaaaa tgttgttctt
     2101 gccaacgtct tttaaatgga acggttctga atatcccatt caatataatt actgcatcaa
     2161 tccgttttgt tgtaattttg ggaaagaaca gcacaaattc aaagatgtaa aagggaaacc
     2221 aagtcgatat aaaatgactg gtagttccaa agataaaggt cataaaggta tgtactgcaa
     2281 tgacaatcct attggcagag gtgtatcgca aaactgtaca gtaacccctt tatctaattg
     2341 gtctgttgtt gaagaaatta aacgccttat agaaatcaat agcatccaag atgttgagcc
     2401 agattatcaa tttcataaag aaggctgctc ggaagaagaa tcaaccccat tcaatgagcc
     2461 gaagcaattt tacaaaaggg ggaaaagtag gggcaaatct caacgctatc aatgtaaggc
     2521 gtgtaagaag ttcactaatg ttctaccaaa aagagaagaa accactactt atcaccaaca
     2581 aaagaacacc attcttccaa tgttcgctaa gatggttgtt ggaagggttt cagtcagtcg
     2641 tacttgtgat attttaggaa ttggagttgg aacctactat cataagctgg agtggctgta
     2701 taggcgttgt ttggagtttt tagaacgata tgagacacaa ccccttcaaa caaagaaatt
     2761 taacgagatg tggcttaata cggacaagat gcactactac ctaaataacg tccgtaagaa
     2821 aggacaaggt tcaaagaaat atacggggtt tgaagacctt aatatgcaaa cgtatatagt
     2881 tgtatctgct gaggtcttat caagatatgt gttccgttct gatgttgcct atgattggaa
     2941 tatttcaatg gatgaattaa atgaagatac aagaaaattc aaagaagacc atctcaatac
     3001 ttttagtcgt aaaaatgata ggttagattg gtcttattat ccacaagaac catccgctaa
     3061 cgatagcgaa aataggaatg cttatcttca cgaattaggg aaaattacta ataggagtag
     3121 atttgtcgat ggtttaaatg ttgatgctcc ttatactaca actgctcact attggttaat
     3181 taaacaaatg gttaatgccg acgaatggcg aatgattagt gatgatgact tctctattag
     3241 aaacgccttt tatagagtat tcacgaaaga attaaggttg agcgatgcac atcattttat
     3301 ttgccaagtt aacaagacga aaagcagaaa acaatgctta aaagaattcg gacaagcaaa
     3361 agcagaactg ctcgattggg gagatatcag aggattcaaa acaaagtttc tacggacttt
     3421 ggcatctcat tatctaactg aattactaac aagtcatcaa ttccacgaag aggctattag
     3481 taaagatgga gaaagatatc gcaaatatgc cgataaccct atcaaacatc cattagccac
     3541 taaggataaa ggattttatt cagttgattg tagaacggat ttatctgctc tggaaccaaa
     3601 cgaaatcgca aagatgctat taaatgttaa tgaccattcg accaatagct ttattcaaca
     3661 gataagacgt tatatatcat ctcttgaaag accattaaca acagctcgtg gtgataagaa
     3721 gagctatatt tacgctaact tcaatccgaa gtatgctcaa tttgcgatta caattctaag
     3781 aacttactac aacttttgtc gcccttttaa atcagcggat aaaaaggttt tgacacctgc
     3841 tcaaagatta gggattacag ataaacaatt tgattggaaa gatataattt atttcaaatg
     3901 acaaaaagag cttggagtaa tcccaagctc ttgtgcttta tctttactgt tcttcttcaa
     3961 ttaacgcacc cgttaactca ataagttttt tctttcatat acatgaaaat agtagttata
     4021 cggattttta tcattcttta tccctttttg ggcaaatacc tcattccatt cctcataatt
     4081 cacttctgga aaaaaagtat ctccttcgaa ttcatgatgt atttttgtga tgtacatttt
     4141 ctcaacataa gggaaaaaca aattataaat ctgttctcct ccgaaaataa aaatttcttc
     4201 ttcgttttta cataactcaa aaacatcttc tattgaatga acaatttcac aaccattaaa
     4261 ggtaaacccc ttatctctcg tcagaataat atttcttctg tcaggtaagg ctcttccgat
     4321 tgattcaagg ttcttcctac ctaatattat cggatgtccc tttgtagtat ttttaacata
     4381 ttcccagtcc ttgggaatcc tccaaggaat gtcattctct ttgccaatca ctctattctt
     4441 atccatcgca gcaatcaaag aaactttcat ttataacaca gcctttttaa ctaatttacc
     4501 atctaatttc ttatttctat caaaaaacct tttctcatta tcctgcccgt tagttgaata
     4561 agcaattttt tctataagtt atattatcat aataccacaa aatcacaaat tttcattcaa
     4621 ttatcatttt caattgcatt ttataaatca ttttgtatgt aatttggtat gtaagttaaa
     4681 atgaaaaaag tagtccattt tgaagtacat tttgtcattt tgtactccaa aactgaacta
     4741 cttagtatag attactgttg cttctttact aatttaggtt tacttgttaa gaaagattat
     4801 ttacgcaaat gttcttcaac aatttgctga gatttatcag ctgtatcttc cacttcatct
     4861 tttgcttttt ctaacttttg aatatattgt ttcaagcttg agttttcagc agttgcttcg
     4921 tctaatttct tttgtgtatc tgttaactga gtttctacat ttgttttttc ttgtgtcagt
     4981 gttgcgattt gttgattcaa accatttaca gtattttgta attctgaaag ttttgcttgt
     5041 gcttcttgta gtcttgctgt ccaatctgca ttcagatgat tgatttcatc atttttttgg
     5101 ttgatctcat ctagtttgac attgatttca ttttgtttgc tttgtagttg tgcatccttg
     5161 ctgactaatt gactttctaa gttttgtttt tctgctgccg attgattttt ttggttttct
     5221 aattcattta cttttgtttg gtattcttgg acatctgcct ttgaatcttg caatgtctgt
     5281 tctttttgtg ctagtcgagc ggtaagatct gtaatcgctt gctctttatc tgtcaatgta
     5341 tgatcaagct gtgtcagatt ctcattgatt gtatcaatat ctgtctctcc accccacaaa
     5401 ataatttcat ttgcgtaccg cgtgcctgtg tagccaatta gacatagaaa tgcaacgaca
     5461 atcccaccag taataaattt ctttttcatt tgcatagctc cttaatccaa tacttttaca
     5521 tgctaaaagt aagtgatctc aaacacaatg tcaagcgttt ttaaattaca tatgtaattt
     5581 taatataaat actatcaaat actcatttaa tcctcctttt ttatctattt aaaaaatctt
     5641 ttttatagag aaaaatgcag gttaatactt tcttaaataa atatttatgg tgaacaaaaa
     5701 caaaaaagca agttttatta aacgtaaccc ttttcatttc atagattact gtatttatta
     5761 caaaaaacg
//
LOCUS       DACGLW010000121         5763 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.129, whole genome shotgun sequence.
ACCESSION   DACGLW010000121 DACGLW010000000
VERSION     DACGLW010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5763
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.129"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            complement(<1..222)
                     /locus_tag="IXT53_002709"
     CDS             complement(<1..222)
                     /locus_tag="IXT53_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HAQ4906475.1"
                     /translation="MQGVIAMMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFE
                     FIYPIVEATYSDLGRPSIDPVILIKLVFIQ"
     gene            430..1404
                     /locus_tag="IXT53_002710"
     CDS             430..1404
                     /locus_tag="IXT53_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ4906476.1"
                     /translation="MRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAVLMAGQYLFD
                     KELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAREFGAMTICL
                     SNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLDGSKKWETCLNEF
                     DKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAACWFMEMQWIN
                     SNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSNKLMVLDQADF
                     DLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY"
     gene            1386..1847
                     /locus_tag="IXT53_002711"
     CDS             1386..1847
                     /locus_tag="IXT53_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAQ4906477.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMAQSAGKSIEEIDFKELLNNSQKSMVHINSLLKDVS"
     gene            1897..2361
                     /locus_tag="IXT53_002712"
     CDS             1897..2361
                     /locus_tag="IXT53_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ4906478.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFG
                     GVSKKSESKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            2375..3124
                     /locus_tag="IXT53_002713"
     CDS             2375..3124
                     /locus_tag="IXT53_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ4906479.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            3124..3954
                     /locus_tag="IXT53_002714"
     CDS             3124..3954
                     /locus_tag="IXT53_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ4906480.1"
                     /translation="MENSTEKKRLITKKDLHKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTPFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
     gene            3954..4601
                     /locus_tag="IXT53_002715"
     CDS             3954..4601
                     /locus_tag="IXT53_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HAQ4906481.1"
                     /translation="MIKALIFDMDGVLIDSEIMYQKWVFNFLKQENLSYPLYSYQKKI
                     GTTASIFDDIEKYNEGCNSLELKGKFTKYWKSQKIDYLDIFPNILIEQLEWLKKKKIK
                     MAISSSSPMDTINEVVTTCKIESYFDCLISGRDLPESKPNPTIFLKASEQLRIPVEEC
                     LVIEDSYNGIKAGKRANMKVLAIKDKRFSQDVSLADDVIYDIKYLRQEVQVKFGI"
     gene            complement(4699..5475)
                     /locus_tag="IXT53_002716"
     CDS             complement(4699..5475)
                     /locus_tag="IXT53_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ4906482.1"
                     /translation="MNFQDNRPLYLQVEASIRNDIREKKYLPGEQLPTEEELCKIYGV
                     SKITIRKAFKLLTESGTVERLRGKGTFVTQKKESLKLAGVHGFSDSLSTRGHKIRYTV
                     LHSKIIKADEFLANKLQINVTDRVYNIKRLMWEDGAPMGIDNFFASEEKFPTLFEKGS
                     QYTSLYKLLEDDYDVKATSSIMEINGLSATPELSDLLQCIVGDPLFILEKVGKDKNNQ
                     PFHYSQSTVRCDRISYVIEVTENITLDTVHKNDSDPKVID"
     gene            5557..5700
                     /locus_tag="IXT53_002717"
                     /pseudo
     CDS             5557..5700
                     /locus_tag="IXT53_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608918.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
BASE COUNT         1971 a          797 c         1024 g         1971 t
ORIGIN      
        1 ctgaataaac accaatttga tcagaatcac tggatcgata cttgggcgac ctaaatcgga
       61 gtaagtggct tcgacgattg gataaataaa ttcaaaatca agcgcggcat caatctttcg
      121 aacgagatga tcttgaggaa ctagatcatc aatcgtaagc atcgcaaatt gatttctacc
      181 attcgtattg tttttagaca tcatagctat cactccttgc attcgttttc ataaatctat
      241 tataaaagaa aagactgtag acatcatcca taaaatggac tttgtctaca gtctggcatt
      301 ttattatata aaattgttat aaaaaatttt ttttgaaaaa tagtattgca aaataaagca
      361 atttcataga taataggtat cgtaaagtta tataacttta taatacctat tagagtgagg
      421 gataaagaaa tgagacaact acacaaagaa cagattgaaa cagcaatggc agcgttaaag
      481 gaaaagacaa ttgatcatgt atattttaca gcatgtgggg gttcacaagc cgttttaatg
      541 gctggtcaat atctatttga taaagaatta actgtacctt gtcatgttta tactgctaac
      601 gaatttaact atgctatacc aaaaggatta tctgaaaatt ctttagttat cacttgttcc
      661 cattctggca caacaccaga aactgtagaa gcaacaaaaa aagcaagaga atttggagca
      721 atgactattt gtttatctaa tgaagaagga tctcctctat gggaagcagc tgaatatcct
      781 gttcactatg attggggtaa agatgttgat gctagtgatt taaataaagg aattctttat
      841 ggtttattat ttaatatttt acttgtttta gatggctcta aaaaatggga aacttgcttg
      901 aatgaatttg ataagttaaa tgatcttgtt attgctgaaa agagcaaatt tgaaataact
      961 gctaaagaat ggggtaaaaa ccaaaaacga gataaaatta tttacacaat tggtagcggt
     1021 attaattacg gagaaattta ctccacagca gcttgctggt tcatggaaat gcaatggatt
     1081 aattctaatg ctattcattc tggggaattt ttccatggtc cttttgaaat cactgattat
     1141 gatgtaccat tcatcttagt gaaaagtatt ggagatacaa gatttttaga tcaacgggta
     1201 gaagacttca ctaaaaaatt cagtaataaa ttaatggttt tagaccaagc tgattttgac
     1261 ttagaatcta tagatagtgt agcgaaagaa tatattgcag ctgtacttag tggcgttgtt
     1321 attcgccaaa tggtagaagc gattgctttt gaaagaggcc attccttaga tgttagacgc
     1381 tatatgtggc aaatgaaata ttaattaata ggaggttgca aaagatggca aatcaattat
     1441 ttcacattgt gattacaaca cattctaccc tttgtgaggg ttatttaaag gcaacaaaat
     1501 taattttaat gaatgacgcc acaggtatta gtgctgttcc atttaaagaa aatactagtc
     1561 cagatgattt tgaggagagt atgaaagaaa taattagtca gcatcatttg caacctctaa
     1621 ttattttaac ggatttaatt ggaggaacac ctaataatgt ttctattaaa cagttgaaca
     1681 acaatagaat tcaaattatt gctgggatta atttacctct tttactggag ttattaatgg
     1741 cccagtcagc tggtaaatct atagaagaaa tagattttaa ggaattactg aataactctc
     1801 aaaaaagtat ggttcatatt aattcgcttt taaaagatgt cagttaaggt ggcaatttct
     1861 attctataaa ttttaaaaat tgaaggagga aatagaatga ttaaattagt gcgtgtagac
     1921 cacagattac ttcatggaca agtaatattt tcttggacta aacagtatga tattaaccat
     1981 attattgtgg ctgataacaa agttccagga gatccaatgt ctgtgatggc tttgacaatt
     2041 gctaaacctg ctgattgcaa attagacatt gttaaaatta gtgatgttaa agggattatg
     2101 gaaacatccc caaatgataa ttacatgatt ttaattaaag gacctaagga agcttttgaa
     2161 ttagttcaaa atttaccaga aattaaagaa attaattttg gcggtgtgtc taagaaaagc
     2221 gagtccaaac aatatggaaa ggcagtcttt ttaactcctg aagaattaga agccacaaaa
     2281 aaaattcttg atcaagaagt gaaaatattt gtgcaacaag tcccatcttc aagtattgaa
     2341 aatgtagatt ttagtaagta aggggggaaa ataaatgtta ttaaaagcca ttttattagg
     2401 tttagttggt gtggtagctg ttattgactc caggttaatt ggtcgacaaa atattggtag
     2461 accactaatt ttgagtacac ttgccggact tgtgctaggt gacttgcata cagggattgt
     2521 tcttggggca tctttagaat taatttctat gggattcgtc tcaattgggg cggctggacc
     2581 tcctaatatg caacttggta gtattattgc cactgctttt gcaattttaa ctaatagtag
     2641 tacagaaaca gccttaacaa tagctatccc agtggctgta gctggtgaat ttatgagtat
     2701 tatcatgcgt atgataattg ctcaatttgg acatgctgca gataaagcca ttgaaaataa
     2761 taaatttaga aaagctcaaa ttattcatat ttattggtca tttattttta atgcttttgt
     2821 ttactttatt ccaatctttt taacagtata ttttggtgct gacgttgtgg cagatttagt
     2881 agataaaatt ccagaaatga ttacagacgc cttgactgtc tctggaaata tgctttccgc
     2941 attagggttt gcaatgctat taagcacaat gttgagtaaa aaattatatc cttactttat
     3001 ttttggattc tttattgttg cctattcagg tctttcacta attggcgtga caatttttgc
     3061 agctctgatt gcctttgtaa tggatcaagt taaatatgga aaaagagaag aggcgcatgg
     3121 ttaatggaaa atagtacaga aaaaaaacgt ttaatcacaa aaaaagatct tcataagatg
     3181 tgtcttcgtt catggacaat catgtgttcg tggaattatg aaagacaaat gcatatggga
     3241 tttatgtatg ggatggcgcc aattttaaat aaagtttata aaaatgatga agagaaaaaa
     3301 agagaagctt atactcgtca tatggcattt tttaactgta cgcctcaaat gactccattt
     3361 attatgggac tttgtgcttc tatggaagaa caaaatgctc aagcagaaga aggaaacttt
     3421 aatactgaat ctatctccat gattaaaacc agtttaatgg gtccttttgc tggaattggt
     3481 gattcatttt tccaaggtac tttaagaatt attacatttg ggattggatt gtcttttgct
     3541 aagcaaggta gtattttagg accgatttta gctgttggcc tttttgctat accttctatt
     3601 ttagctgttt tttacggcgg taaattagga tatgagtctg gcaataaata ccttactaaa
     3661 ttgtatcaag aaggtcttat ggatcgagta atgcaatttg cttctatcat tggtttggct
     3721 gtagtaggtg gaatggtggc tagcatggtg ccaattgtca cccctttaaa attcactaca
     3781 gggggaacag aacttgtttt acaagactta cttgattcca ttattcctgg attattacca
     3841 cttgtatgta ctttgggaat atataaattg attaagaaaa atgttagtac aaatatgctc
     3901 ttattaggtt gtattttggc agggtttgtt ttaagtataa taggtatctt ataatgatta
     3961 aggctctaat ttttgatatg gacggagttt taattgatag cgagattatg tatcaaaagt
     4021 gggtatttaa ttttttaaaa caagaaaatt taagctatcc tttgtattct tatcaaaaaa
     4081 agattggaac tacagcaagt atttttgatg atatagaaaa gtacaatgag ggatgtaaca
     4141 gtttagaatt gaagggaaaa tttactaaat attggaaatc gcaaaagatt gattacttgg
     4201 atatttttcc taatatacta attgaacagc ttgaatggtt aaaaaagaaa aaaataaaaa
     4261 tggcaatttc ttcttccagt ccaatggata ctataaatga agttgttacc acttgtaaaa
     4321 tagagtcata ttttgattgt ttaattagcg gaagagattt gccagaaagt aaacctaatc
     4381 ctactatatt ccttaaagct tcagaacagc tcagaattcc agttgaagaa tgtctggtga
     4441 tagaagattc ttataatggt ataaaagctg gtaaaagagc aaatatgaag gttcttgcaa
     4501 ttaaagataa acgtttttct caagatgtaa gtctagcaga tgatgttatt tacgatatta
     4561 aatatctgcg acaagaagta caggtgaaat ttggaatata aataagtttt ttgatgtatt
     4621 tagagaatat tagtaaaaat aaaaatcatg cccaaatcta atcaaaattg attgctttgg
     4681 gtatgatttc ttttaatatc aatctattac tttaggatct gaatcatttt tatgcaccgt
     4741 atctaaagtg atattttctg tgacttcaat aacataacta attctatcac aacgaactgt
     4801 ggattgagaa taatgaaatg gttgattatt tttatctttg ccaacctttt ctaaaataaa
     4861 tagcggatct cctactatac attgcaataa gtcggaaagc tcaggtgttg cagataaacc
     4921 gtttatttcc ataatagagc tcgtagcttt gacatcatag tcatcttcta gaagtttata
     4981 taatgaagta tattggctac ctttttcaaa taaagttgga aatttttctt ctgatgcgaa
     5041 aaaattatca attcccatcg gggcaccgtc ttcccacatt aaacgtttaa tattgtaaac
     5101 tctatcagtt acattgattt gtaatttatt agctaaaaac tcatcagcct ttataatttt
     5161 agaatgtaaa acagtatatc taattttatg tcctcgggta gaaagagaat cgctaaatcc
     5221 atgaactcca gctaatttta aactttcttt tttttgagtt acaaaggtcc ctttccccct
     5281 taatcgttca actgttccac tttctgttaa aagtttaaaa gcttttcgaa tggtaatctt
     5341 acttactcca taaattttac ataattcttc ttctgtaggt aattgctctc ctggaagata
     5401 ttttttttca cgaatatcgt tacgtataga agcttctact tgtagatata atggtctatt
     5461 atcttgaaaa ttcatagtac actccctaaa aagttatact tcattatatc acaatttatt
     5521 aaaataaaag attaagatat aaatataaat gaactagtct ttgactaacc aatgcccgct
     5581 tttgaaagaa aacaaattca ttctgtttta agtaaaaatc cttatgttac cactcattct
     5641 gaaggggatg agccttatcg ctacttggtt gtggaaccaa gcgataagac aattttataa
     5701 attcaatatt aattaaaata aaaacaaaca aagtagatta ttttactttg tttgtttttt
     5761 tat
//
LOCUS       DACGLW010000122         5727 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.130, whole genome shotgun sequence.
ACCESSION   DACGLW010000122 DACGLW010000000
VERSION     DACGLW010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5727
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.130"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            196..921
                     /locus_tag="IXT53_002718"
     CDS             196..921
                     /locus_tag="IXT53_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906483.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHSQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            911..1420
                     /locus_tag="IXT53_002719"
     CDS             911..1420
                     /locus_tag="IXT53_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906484.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(1490..2938)
                     /locus_tag="IXT53_002720"
     CDS             complement(1490..2938)
                     /locus_tag="IXT53_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HAQ4906485.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(2938..3654)
                     /locus_tag="IXT53_002721"
     CDS             complement(2938..3654)
                     /locus_tag="IXT53_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HAQ4906486.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            complement(3635..3985)
                     /locus_tag="IXT53_002722"
     CDS             complement(3635..3985)
                     /locus_tag="IXT53_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HAQ4906487.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            4129..4902
                     /locus_tag="IXT53_002723"
     CDS             4129..4902
                     /locus_tag="IXT53_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HAQ4906488.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMDNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            5073..5439
                     /gene="ssrA"
                     /locus_tag="IXT53_002724"
     tmRNA           5073..5439
                     /gene="ssrA"
                     /locus_tag="IXT53_002724"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         1662 a         1124 c          994 g         1947 t
ORIGIN      
        1 aaaattattg tcgctgctcc tcttttcgaa taaacagtgt tcaaaagctt gcatctacgg
       61 taatagaata gggcagcttc ttcacaacct cttgcttttt acaaaatatt ttctggtttt
      121 aattcgtgat ttcactaaag gaatttcata ctttattcat attcattttc tatacttttt
      181 atagaggtga aaagtatgaa aataatccgc tatgctactg ctcgattaat ttattacaaa
      241 aagcagacaa tactctattg tttattttca agcttttctg cttttttatt gatagcatgc
      301 ctgactcttt accattcgca atcggcttta gccaatcaag tccaagatcg gctttcattt
      361 attggcgctt ctgcatctac agagcttctt ccggctttag aattagctag ccgattttat
      421 ataaaaggca tattcagttt attttttcta tttgccttcc tttttatctt tttcttttac
      481 tattcagtca ggcaaaacaa gcaggaattg atcaattggc gcctgactgg tctttctaaa
      541 accaaattat tcctttttat tttctggcag ctgttctttc ctctctttct ttgttgtatc
      601 ttggttttat tgtggatcgt cagcttccag tcagtttatg aagcgcttct tcaaaggatc
      661 aattttcttg tcttaaagac tactgaactg ccagatatcc gtgatatctt gacttcggta
      721 agcggactta caattccgat ggatcaacag acttttttta ccatcgattt ccaaaatgat
      781 ctttttttca cagatatact taagggattt gcccaaactc ttattgggct cagtggatgc
      841 tgtctcattc ttagtctatt ccctttcact ttttttatcc atcatttgca aagtaggagg
      901 cctggacgta atgaaacctg aatatttgat tcatgctgaa cacttgaccg ttattttgtg
      961 tcctctcgaa aaacagcata aaatattgga gcacttgaaa aaagaggttc ttgttgcaaa
     1021 actggataat tttgcgatca tccaaaaaaa ttggccgatg tttccttatc tgaatcttaa
     1081 agaccatgtc ctgttggatg taccggaaaa agaaatcaaa caagatcgtt tggcctatca
     1141 agaaaaactt gatatctctc cttccttgct taattgtgcg gcagctgagt tgacgtcttt
     1201 tgaaaaagta aagctgcaat tacttcatgc cttgctttcc aagcgaaaca atattgttat
     1261 tgaagataca tttgatgaac ttagtgtatt ggagatccaa gagctactcc acttgctttc
     1321 ttatttagct catgaagaaa atcaaggtat cttactcttt actcatgatg ctactattgc
     1381 tcaaagtcct tatattgatc gtttagagcc cgcgggctaa atcaatcatt ttatagtaaa
     1441 aacaagcggt atagaagatc gattcatgat cttttatacc gcttgttttt tactcttttt
     1501 tgatttctac gtcaggcaaa cgaacagcga attttgttcc ttttcctagt tcgctttcta
     1561 cttctaattt tcctttatgc aagcgtacaa gttgctgcac gattggtaaa cctaatcctg
     1621 attcaccata ttttgtattt tttcttgaag gatctacttt ataataacga tcccagatac
     1681 ttttcatttg ttgttcagac attccaattc ccgtatcttc aatcgtgatg atcgtttcaa
     1741 gatagccttt ttctaaagta atcgtgattt ggccgttttc agtaaactgt atcgcgtttt
     1801 gaataatatt gaccatgatt tggacaaatc ggtcataatc agcgtaaaca tcaatatcat
     1861 caacggcttt caatgtcaat gtatctcctg ctgcttctgc tttagcttct aactgtgcaa
     1921 caatattttt caatgtttct gttccattga attttttaat gaccatactg atttgattcg
     1981 tacggatttt ttcataatcc aagttttcat tcaccaaacg gatcagacgc tctgtttcat
     2041 ttttcatcaa tttaatcgcg ttttctcgct gattttcagg gattgcattg tattctaacc
     2101 cttccagaag tccgttgatt gtcgtaagag gtgttctcat ctcgtgagaa gcatctgcca
     2161 taaattgtct tcgacgattt tcttgttctt caatttctgc ttgagattct ttcaatgaat
     2221 tcgtcatttt attgaaatca tctgctaact catcaaattc atctttgtca tgtacgggga
     2281 gctgtacatc aaaattacca ttcgtcactt ctttcgtcgc attccttaac cgattgatac
     2341 gcttaacttg gaatgctgca aaagcatagc tggcaataac tgcaacgata ctggaaaaaa
     2401 taaatccttt gaacaggttc agtgtaattg gacgaacgct gttatcgata tttcgcgcag
     2461 gttgactaac tacaagtgct ccatagaatt catgattcaa attaaatggc acaagtgcgt
     2521 aagaggtcgc ctgtgcttgt cctaaaatat ttttattcga tgtgaatttt tcttgacgtc
     2581 cattcctcag atttttccat tgatcagctg ttaaggtaaa attcagtaaa gctgtattag
     2641 tcggataaat gacacgttcg tttttatcga taaatataaa gttcacattt tgttctgtca
     2701 gtacttgctc tgtcagcagc aatgcatttt gaaacgcctg atcttgacta aactgcggga
     2761 gcgtatcggc ataggtttgc gttgtctttt caactgattc agcatatcca aacagttgct
     2821 ggtaattgtt ttcttcaatc gtttgttttg tcagttgtgt aaaagaaact ccaacgatca
     2881 ataagatcaa cgcgaccatt ccgcaaaaag ctaagagttg ctgatacaaa taacgcatta
     2941 ggcaacacca gaatcatcga atttgtagcc gactccccaa acagtttgga tgacttgtgg
     3001 tccgaccttt tcgatttttt gtcgtaattt cttgatatgg gcgtccactg tccgttcatc
     3061 accaaagtac tgataatccc agactaattc aagcaactgc tcccgagaga atacttgacg
     3121 tggttttttt gctaaagtaa acaatagatc gaactcttta ggcgtcagtc cttcaattgg
     3181 ccggttgtcc agatacgctt ctcgtgtttt cgtattcatc ttgaaatgac tagtctcgat
     3241 atcaaaatga tcatctgaaa ctgctgcttc ttctcttgct tctgccaact cactacggcg
     3301 atgcaaagct ttcattcgag caataagcgt cagaggactg aaaggttttg tgacataatc
     3361 atctgccccc atctctaagc cgatcacctg atcgctttca gaatctctag ctgtcagcat
     3421 gataatcggc acagtacttg aaactttgcg gatctctctt cctactagca taccgtccag
     3481 tgtgggcaaa ttcaggtcta gtgtaatcat atcccaatgt tgtgggtttt ctaaaaaggc
     3541 atcgagccct tcttttccat catacttgaa agttgcttcc caaccttcat ttaagaaaaa
     3601 catctgcatc atttcagaaa cagattgatt atcttcaatc attaaaatat tcattatcgt
     3661 tccctccagt ttccaggtca tatttctgta atttcttttc tgtcataata aaaataggca
     3721 taattggaaa tactagcaga agcatttcaa tcagaaaata attcgtatgc ccagatgtct
     3781 ttaaataata gccgatcaat gccatcagta agcccataag aaaaaaccag tttccgcttg
     3841 tcttttgagc cagccgccaa tgagcgtctg tttttgcggc aagaggcgaa tgatacccat
     3901 aaaaaggcag atttcctttt gagggcagaa tccgaaaaag actgccaatt attaagaata
     3961 agataccaat actgaaaaaa atcattcttt ccctcctaaa ttctcttctt atagtatact
     4021 ctaaattgtt gactgaacca atatgttcag gaattcttca cgaaaggttt gtcttttgtt
     4081 cattctttgt taagataaac ttattccaat aaaaaaggag ttttatttgt gtcaaattat
     4141 cgtgcaatca ctttctttga tcttgatgga acattactag atgcgaaatc aaaaatcaca
     4201 cccgaagtgg caaaagctat gaacgcatta aaagaaaaca atgttctccc cgtcatcgct
     4261 acaggacgta cagaagcaga aatccactct atcatggaag atgcgggcat tacttctgcg
     4321 gtcaccatga atggttcttt cattcgagta gacggcgaag aaatctattc tcaaacattt
     4381 tcttcagaag aatgtcaaaa aatgtatgat catgtacagt tgaacggtca tgaactttct
     4441 tactataacg atcagaaaat ctggtgtacc ggtcatagcc aaacaatgat caatgcttac
     4501 gacttcattc attcagatgt tccggaaatt gatcctttag gctttaaaga aaacacagta
     4561 aatatgttgc tgatcttatc tcaatcaggt gacgaatact atcacgaaca ttttcctgag
     4621 ctaacttttt atcggaatgg tccttactcg atcgatacag tgaagaaagg aatctcaaaa
     4681 ggggcaggtg tcaaacgtct gcaaaaagaa cttcagctgg aaaaagttcc tacttttgga
     4741 tttggcgatg gtccgaatga ttttgccttg ttggaagctt gcgacaacaa aatcgctatg
     4801 gacaacgcct atgacggatt aaaagaactt tctaccttta tcacaaagaa aaatacagaa
     4861 ggcggaattg tccacgcatt gaagtatttt gatctgattt aagatctttt tttgaagcta
     4921 cctcttttct taagaaaatc gcttattttt tcttaagaaa ggggcagtta ttatataaat
     4981 aatgggatat gattcgcttt tttattgaat ttatcccaat atatgctata ctaattttgt
     5041 atgaaaaata cacgtaaccc ttgttagcta acggggacgt tacggattcg acaggcacag
     5101 tcgagcttga attgcgtttc gtaggttacg tctacgtaaa aacgttacag ttaaatataa
     5161 ctgctaaaaa cgaaaacaac tcttacgctt tagctgccta aaaacagtta gcgtagatcc
     5221 tctcggcatc gcccatgtgc tcgagtaagg gtcctaactt tagtgggata cgtttcaact
     5281 ttccgtctgt aagttgaaaa agagaacatc agactagcga tacagaatgc ctgtcactcg
     5341 gcaagctgta aagtgaatcc ttaaatgagt tgactatgaa cgtagatttt taagtggcga
     5401 tgtgtttgga cgcgggttcg actcccgccg tctccatata tgtatggttg agacagataa
     5461 acatgtatga aaaacgttgt taaatcaacg tttttttatt ttggtcaagg agagaaaagt
     5521 agagaaatac aaaatttttt gcattgattt tgcattcaaa aaaacattca aacatttaga
     5581 aatccccaat acgtattcag gaaaatctat tttaaggttt acaaaaagaa cttttgttcg
     5641 tacactcttt ttgaggtgaa cttttatgct gatggaagga aaaacacaat tatggtttaa
     5701 gcatgttcct tcaaataagt tttttta
//
LOCUS       DACGLW010000123         5709 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.131, whole genome shotgun sequence.
ACCESSION   DACGLW010000123 DACGLW010000000
VERSION     DACGLW010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5709
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.131"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            complement(53..787)
                     /locus_tag="IXT53_002725"
     CDS             complement(53..787)
                     /locus_tag="IXT53_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HAQ4906489.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1114..1524
                     /locus_tag="IXT53_002726"
     CDS             1114..1524
                     /locus_tag="IXT53_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HAQ4906490.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1517..2218
                     /locus_tag="IXT53_002727"
     CDS             1517..2218
                     /locus_tag="IXT53_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HAQ4906491.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2218..3804
                     /locus_tag="IXT53_002728"
     CDS             2218..3804
                     /locus_tag="IXT53_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HAQ4906492.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3823..4044
                     /locus_tag="IXT53_002729"
     CDS             3823..4044
                     /locus_tag="IXT53_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HAQ4906493.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4041..4802
                     /locus_tag="IXT53_002730"
     CDS             4041..4802
                     /locus_tag="IXT53_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HAQ4906494.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5182..5691
                     /locus_tag="IXT53_002731"
     CDS             5182..5691
                     /locus_tag="IXT53_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HAQ4906495.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
BASE COUNT         1764 a         1033 c         1174 g         1738 t
ORIGIN      
        1 aaaaaaactt ggtacgcaat cgctgtacca agttttttta tttagctttt gattaagcca
       61 taaataataa tttatataag aatactgcta cgacaccagc acagataggt gctacaactg
      121 gtacccatgc atatccccat tgagaagaac ctttatgttt caatggaaga agtgcgtgta
      181 caagacgtgg agcaaagtcg cgggctgggt ttagtccagg acctgtagga ccaccaagtg
      241 aagcaactaa tgtccataca aggaaaccaa gtgctaagtg agctgtacca agattatctt
      301 ggaagaatgg tgcttttgta atacctaggg caccaaagaa taaaacgaat gaaccaaaga
      361 attcattgac gaaaccattg aatttagaac caacgctgtt gattgttgag aatgtaccta
      421 acacatggtt tacgtcttct gtttggtcgt agtaaggttt gtaacaagct actacgatca
      481 attgtccaat cgctgcacca atcaattgtg caatgatata aggaacaact tcagcccaag
      541 ggaatagacc attagatgct aaagcaattg taaatgctgg gttgatgtgg ttaccgctga
      601 ttccaccaaa aatcattgct ggcatcataa ccccgaatcc atacccgaca gcaatcagca
      661 tccagtcact gccatatcct tttgtacctt ttaaatctac gtttgctaca gcaccgttac
      721 ctagaacgat tagtaatgct gtaccaatca tttccgccgc aagacgggtc attagtgagt
      781 aatccataaa tacctccaaa agttataaaa atgcaaaaaa aaaagaaaca tcaacacgaa
      841 aagcatcggt gcagtgtttc gtgtcggaag gatttctgca gtcttaacaa atgtgaataa
      901 aaaaatgaat tccattactt ataagaaacc agaccaacag taaaactcca actcttttta
      961 aaagttctcc acaaatcacc aagcttaact ataaatatac caaaaaaaaa acgttttttt
     1021 tctcaaaaat tgtatcttag aaacagcatg ttttgaaact ttcagaaaag gtttgttagt
     1081 aaaatgaaag ctatacgtaa ggagtgttgt ttgatgaaat tttttcaaga gtttttcatt
     1141 attattttct tttctgtatt aggagaaggc atacggattt ttgctcattt acctattcct
     1201 ggaagtatct taggtattct ttttttattt ttagcatttg aaatgcatct tgttgatcct
     1261 gaaaaaatcg gagcaactgg tgatttttta ttgaataatc tagccatttt atttgttcct
     1321 gctggtgtag gtttattgga atattttgat gatattgctt cgatatggcc tgttttactg
     1381 ggagcagtcg tcgtgtgttc catcgtcacg atggttgcag cgggaaaaac ggcagaaggt
     1441 gtagaagctt tgctgggcta tgtccgtaag aaaaaagcag aaagggtatc tgtaaacgaa
     1501 atggaggaga atcaaattga ctgagttttt tgtgaatcca ttatttggct taagtttgac
     1561 tttaggagtg tacgtattat ctgacaaatt tttgcggaaa tataaactgc ctgttttgaa
     1621 tcctctcgta ttatcgattg ttgttattat cttatttttg catttttctg ggatcagcta
     1681 taaagactat aatatcgggg gcaatttttt gactatgctg attactcctg cgacagtggc
     1741 acttgccatc cctctttata aaaacttccg tttactgaaa gagaattttt atcctgtaat
     1801 ggctgcgatt ttagcaggaa tagtcgcaaa ttgcttggtt agtgtagggt tcggttactt
     1861 cttctctctt cataaagaga tggtgatctc attactacct aaatctgtaa cgacggctat
     1921 ttctgtcgat ttatctcata cgatgggcgg ggtgaatgcc gtgacacttg ctattgttgt
     1981 ctctacaggt atttttggtt cgctgatcgc cactcatatt ttccgcctat tcaagatcga
     2041 aagtccagtt gctcgcggtg tggcgttagg aagtacttct catgctattg gtacagcaaa
     2101 agcaatcgag attggtgaaa tagaaggaat catttccgga cttgcgatat gcgtcaacgg
     2161 aatattgacc gttttattgc taccgttatt ctttcagcct tttgctggct tgttttgatg
     2221 cgaaaaccac tattttatgc tacaataacc aaaacgttcg agcataaaaa aggagggaaa
     2281 gacgtgtatt tgtcttccga gtatcttaaa caatttgata aagatctata ttataagatt
     2341 ctcctcctag aaagttttga ggatcaagct tggcataccg ctgcacaatt agcgcaagtt
     2401 gttcagctgg atgcccgcag cgtgtcgaaa tacctgaacg agctgtctaa aaactatcag
     2461 cagtttagcg gtaagacaca ccctttgttt acaaaaaatc accgttctgg ttataacttt
     2521 tatgatacgc tcgattcaat tgaacatgag cgttttttga tctatctcgt gcaaagtacg
     2581 ctgaaattcc agttgctgca cgacattttt tttgaagaat tccatacaat gtaccaattt
     2641 gcgcaaaagc attacattag tgaatctact gctcatcgaa aaatcaatga atggaaacag
     2701 caactgcaaa cttacggaat tcggctgcaa cgaggtacat atatcgctca aggcgaagaa
     2761 gaaatcattc gactttactt gcatatgaca ttctggcagt tgtttcgagg gaaaatctgg
     2821 ccatttgaaa cgatttctca aatggacgta aagaatatgg cagaacatat catggctttt
     2881 tttaatgttc gtctgaacga gatcaaaaaa agacgattgg aatatatgct aggtgccttt
     2941 tttttaagaa aatcacagaa gcattatgtt gtattgaatg agaaaaaaag acggctgatt
     3001 tctgacaatc tgttgttcca gcgattttgc caggtcatgg agccggtatt tcctaattat
     3061 tttcaagtag aagacgagtt aggcgcatta tttcttgtac tgatgactcg tgaagaatat
     3121 tatagtgatc ctaagatacg gaagaaaatt tttgattttc atcaacaagc aaaaacaccg
     3181 ccctttacag ccctttcaga agcaaaagcc gctctttctc tttatcagga agagcagggg
     3241 ctgccagcag aaaatctaac cttcgaggca gaaaactatt tattcagcag tcattttttc
     3301 gcctatcttt ttcctaatgc gaaagaaacg attgatggca atagtagtga ttttattaat
     3361 catttagtta ttgaaaacaa ggaattgaaa caatggctcg tacatttttt tgaaagtaga
     3421 cacaaacatc ccaatcatct ggcatttaaa aatcatgcgt ttttgatggg gcggtatctg
     3481 actgtgttta aaacattagg agcctttact ccacagcttc caaagatcac gatcctttta
     3541 atgacagatt tcccattatt tgaagagcaa ctgttagagg agggattacg gacatttttc
     3601 cgtaatgagt atcagctgat ttttctgcca acagattatc gggggagaga agttgatctt
     3661 ctgatttcga caagtaaagt ccaccgcaaa ccatgggcgg atcttgacta ttttattgtg
     3721 actgaagaac taaaattaat tgattacatc caactatctc aaaaatttga aatgattcaa
     3781 aaacaaaaac tattaaaaca ataacaaaga aggagaaaaa agatgacatt tgaagaaatt
     3841 ttaccagaat tgaaaaaagg tgcaaagatt attcgtaaag gatggagtgg atttgaactt
     3901 tatgttgttc tcgtatccga tgatgaattt gatggctgtc cggtgacgcc gtacttattg
     3961 atcaaaacaa gtgacgaagg tttttccagc ttcgctccta cagtttgtga tattttagca
     4021 gatgattggg cgatcgtaga atgaaaaacc agtatccaga tctagctaaa caggttgtat
     4081 ttatcacagg agctgcttct ggaattggtg ctgcgcaagc ccgagcgttt cttgaacaag
     4141 atgcttttgt atttggatta gatcaaaagt tcggtaaaat ggaagaaatt tctttgcgat
     4201 ttcctgaaac ttttgcctac gcactaggtg atgttcgaca aatgaaagaa ctccaacaag
     4261 cggtatcgaa atgccaaaat cattttggtg aagttacgat tttactgaat acagcgggga
     4321 tattggatgc ctacaaaccc ttgatggaaa ctgacgaatc gctgtgggat ctgatttatg
     4381 agacgaatgt gaaaagcatg tatcagctga caaaaatcat ccttcctgat atgatccgcc
     4441 aaaaaagcgg aacgatcatc aatatggctt cgattgctgg cttgattgct ggtggaggtg
     4501 ggatcgctta tacaagcgcg aaacatgcga ttgtcggatt taccaaacaa ctcgctctgg
     4561 attacgcagg acagggtatc cgagtaaaag gaattgctcc aggagccatc caaacaccaa
     4621 tgaatgcggc tgattttgaa gggaaaggtg agatggcaga gtgggtggca aatgaaacac
     4681 cagtcaagcg atgggcacag ccggaagaag tagctgagct gacgttattt cttgcctctc
     4741 ctcaagcaag ctacatacaa ggaacgatca tcccaatcga cggcggatgg ttgttaaaat
     4801 aaccttgtgt ttttttcaag cttctgtatg ataaaactaa agagagaaac aacggttgat
     4861 gataaaaaaa gtcgatgact gttcccgaca ttcgatgccg aacagccagt tcaaaaaatt
     4921 ctttttaata atcgttaata ttcgaaacta agcggctttt cacgacctct tgatataaac
     4981 gatattcaaa agcttgcaac tgcggtaaca agtccagcca aatgaaaaat ataaggagct
     5041 atttttattt gaccattctt attacttgtt gcagggcagc tttttcacta ccttctgcga
     5101 cacttcagtt ggaattttct tcccatcctt tggtcgcggt cattaccaag ataaaattat
     5161 cttgcaaagg agaaatagaa catggaaaaa aataataccc gttcagccaa acgctggaca
     5221 acgacagatg tgactcggat ggcgctagtc actggtttat atgtggcagt gacgctggtt
     5281 ttgtctgtct ttagctttgg agtgattcag attcgtctct cggagatgtt caactatctg
     5341 tccttgtata acaaaagata catcgtcgcg gtaacagcgg gagtagcttt ggctaatatt
     5401 gcttctcctc ttggactgat agatgttgca gtaggaagcg tcagcacgct gcttgtattg
     5461 cttatcaact ataaaatcac tagtcgcatc aaaaacatga aaatcaaaat ggccgtgaca
     5521 gcatgcgtat ttgctttttc catgtttaca gtagctggtc agctaacgat tttatatcaa
     5581 ctaccttttt tcttgaattg gtgggtgatt gctttaggag aattggcatc aatgattgtc
     5641 ggtgggataa ttatttattg gatcggtaaa aaagtggatt tgtcaaaata aagatctgtt
     5701 taatttaaa
//
LOCUS       DACGLW010000124         5692 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.132, whole genome shotgun sequence.
ACCESSION   DACGLW010000124 DACGLW010000000
VERSION     DACGLW010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5692
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.132"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            complement(44..2968)
                     /locus_tag="IXT53_002732"
     CDS             complement(44..2968)
                     /locus_tag="IXT53_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HAQ4906496.1"
                     /translation="MKIARGRELLTPEQRQAFMQIPEDEWILGTYFTFSKRDLEIVNK
                     RRREENRLGFAVQLAVLRYPGWPYTHIKSIPDSVIQYISKQIGVSPSSLDHYPQRENT
                     LWDHLKEIRSEYDFVTFTLSEYRMTFKYLHQLALENGDAIHLLHECIDFLRKNKIILP
                     AITTLERMVWEARAMAEKKLFNTVSKSLTNEQKEKLEGIITSQHPSESNKTILGWLKE
                     PPGHPSPETFLKIIERLEYIRGMDLETVQISHLHRNRLLQLSRLGSRYEPYAFRDFQE
                     NKRYSILTIYLLQLTQELTDKAFEIHDRQILSLLSKGRKAQEEIQKQNGKKLNEKVIH
                     FTNIGQALIKAREEKLDVFKVLESVIEWNTFVSSVEEAQELARPADYDYLDLLQKRFY
                     SLRKYTPTLLRVLEFHSTKANEPLLQAVEIIRGMNESGKRKVPDDSPVDFISKRWKRH
                     LYEDDGTTINRHYYEMAVLTELREHVRAGDVSIVGSRQYRDFEEYLFSEDTWNQSKGN
                     TRLSVSLSFEDYITERTSSFNERLKWLAANSNKLDGVSLEKGKLSLARLEKDVPEEAK
                     KFSASLYQMLPRIKLTDLLMDVAHITGFHEQFTHASNNRKPDKEETIIIMAALLGMGM
                     NIGLSKMAEATPGLTYKQLANVSQWRMYEDAMNKAQAILVNFHHKLQLPFYWGDGTTS
                     SSDGMRMQLGVSSLHADANPHYGTGKGATIYRFTSDQFSSYYTKIIHTNSRDAIHVLD
                     GLLHHETDLNIEEHYTDTAGYTDQIFGLTHLLGFKFAPRIRDLSDSKLFTIDKASEYP
                     KLEAILRGQINTKVIKENYEDVLRLAHSIREGTVSASLIMGKLGSYSRQNSLATALRE
                     MGRIEKTIFILNYISDESLRRKIQRGLNKGEAMNGLARAIFFGKQGELRERTIQHQLQ
                     RASALNIIINAISIWNTLHLTTAVEYKKRTGSFNEDLLHHMSPLGWEHINLLGEYHFN
                     SE"
     gene            3114..3689
                     /locus_tag="IXT53_002733"
     CDS             3114..3689
                     /locus_tag="IXT53_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016204422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ4906497.1"
                     /translation="MRKIGYIRVSSTNQNPSRQFQQLNEIGMDIIYEEKVSGATKDRE
                     QLQKVLDDLQEDDIIYVTDLTRITRSTQDLFELIDNIRDKKASLKSLKDTWLDLSEDN
                     PYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKL
                     YKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            3903..4598
                     /gene="vanR"
                     /locus_tag="IXT53_002734"
     CDS             3903..4598
                     /gene="vanR"
                     /locus_tag="IXT53_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAQ4906498.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            4576..>5692
                     /locus_tag="IXT53_002735"
     CDS             4576..>5692
                     /locus_tag="IXT53_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ4906499.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVEL"
BASE COUNT         1754 a         1077 c         1077 g         1784 t
ORIGIN      
        1 aaaatacttt gtttttttac ctgtctactt gacagggggc agttcactct gagttaaaat
       61 ggtattctcc tagtaaatta atatgttccc aacctaaggg cgacatatgg tgtaacaaat
      121 cttcattaaa gctacctgtc cgttttttat attcaactgc tgttgttagg tggagagtat
      181 tccaaatact tatagcattg ataattatgt ttaaagcact ggctctttgc aattgatgct
      241 gtatggtgcg ttctctaagc tcaccttgtt ttccgaagaa aatagctctt gccaatccat
      301 tcatggcttc tcctttattc aatcctcttt gtatttttct tcttaatgat tcatccgata
      361 tataattcaa aataaagatc gttttttcta ttcggcccat ctcacgtaag gctgtagcta
      421 agctgttttg tcttgaatag gaacctagct tccccataat aagggatgct gaaactgttc
      481 cctcccttat agaatgagct aatcgcaaaa catcctcata attttcttta atgacctttg
      541 tatttatttg tccacgtaaa atggcttcta gttttggata ctcacttgct ttatctatcg
      601 taaataattt tgagtccgat aaatccctta ttcttggggc aaatttaaat cctaataaat
      661 gagtcagtcc gaatatttgg tcagtgtaac cggcagtgtc tgtataatgt tcctctatgt
      721 ttagatccgt ctcatgatgt aacaaaccat ccaaaacatg aatcgcatct cttgaattag
      781 tatgaataat ctttgtgtag taagaagaga attgatcact tgtaaatcgg tagatggtgg
      841 ctccttttcc agttccataa tgtggatttg catctgcatg tagtgatgaa acacctagct
      901 gcattctcat accatctgac gaagatgttg taccgtcgcc ccaatagaaa ggcaattgta
      961 atttatgatg aaagtttact aatatggctt gggctttatt catggcatct tcatacatgc
     1021 gccattgaga tacattggct agttgcttat atgtaagtcc gggtgtggct tcggccatct
     1081 tgctcaagcc aatattcatt cccattccta aaagggcagc catgataatg attgtttctt
     1141 ccttatctgg ttttcgatta ttggaagcat gagtgaattg ctcatgaaat cctgttatat
     1201 gggccacatc catgagtaaa tcagttaatt ttattcttgg tagcatctga taaaggcttg
     1261 cactaaattt ttttgcttct tctggaacat ctttttctaa gcgtgcaagt gatagctttc
     1321 ctttttcaag agaaacccca tctaacttat tggaattggc agctaaccac tttaaccttt
     1381 cattaaagct gctggttctc tccgttatat aatcttcgaa tgataaacta actgataatc
     1441 tcgtattccc cttcgattga ttccatgtat cttccgaaaa caaatattcc tcaaaatccc
     1501 tatattgtct gctgccaaca atggaaacat ctcctgcccg aacatgctcc cgaagttctg
     1561 ttaaaacagc catttcatag taatgacgat taattgttgt accatcatcc tcgtataaat
     1621 gtcttttcca tcgttttgaa ataaaatcca caggtgagtc atcaggcact tttcgctttc
     1681 cagattcgtt cattcctcgg ataatctcaa cagcttgtaa aagtggctca tttgcctttg
     1741 tagaatgaaa ttccaatact cttaatagcg ttggcgtata ttttcttagt gaataaaacc
     1801 gtttttgcag taagtctaaa taatcatagt cggcaggacg tgcaagttcc tgagcctctt
     1861 ctactgaaga gacaaaggta ttccattcaa taaccgattc taaaacctta aaaacgtcta
     1921 atttttcctc tcttgcttta attaatgctt gtccgatgtt cgtaaagtgt ataactttct
     1981 catttagctt tttaccgttt tgtttctgga tttcctcttg agccttacga ccttttgata
     2041 acaaactaag tatttgccta tcatgaattt caaacgcttt atccgttagc tcctgagtaa
     2101 gttgtaataa atagatggtt aatatcgaat aacgtttatt ttcttgaaag tcacggaatg
     2161 catacggctc gtatcttgag cctaagcgag acagctgcaa caggcggtta cggtgcaaat
     2221 gactaatttg cactgtttct aaatccattc ctcgtatgta ttcgagtcgt tctattattt
     2281 ttagaaaagt ttcgggtgaa ggatgacccg gtggctcttt taaccaaccc aatatcgttt
     2341 tattggattc ggatggatgc tgcgaggtaa taatcccttc aagcttttct ttttgctcat
     2401 ttgttagaga tttactaacc gtattaaata gcttcttttc agccattgcc cttgcttccc
     2461 acaccattct ttcaagtgta gtgatagcag gcagtataat tttgtttttt cttagaaaat
     2521 ctatgcattc atgcagtaga tgaatggcat caccattttc caaagctaat tgatgaaggt
     2581 acttaaatgt cattcgatat tcactcaggg taaaagttac aaagtcgtat tcacttcgaa
     2641 tttctttcaa atgatcccaa agtgtatttt ccctttgagg ataatgatca agcgaggatg
     2701 gactaacacc aatctgtttc gatatatatt gtatgaccga atctgggatg cttttgatat
     2761 gagtgtatgg ccaaccggga taccgaagaa cagctaattg aacagcaaat cctaaacggt
     2821 tttcttccct ccttcgctta ttaactattt ctaaatcccg tttggaaaaa gtgaagtagg
     2881 tccccagtat ccattcatct tcagggattt gcataaaagc ctgtctctgt tccggtgtaa
     2941 gcaattctct acctctcgca attttcattc agtatcattc catttctgta ttttcaattt
     3001 attagttcaa ttatatatca atagagtgta ctctattgat acaaatgtag tagactgata
     3061 aaatcatagt taagagcgtc tcataagact tgtctcaaaa atgaggtgat attttgcgga
     3121 aaatcggtta tattcgtgtc agttcgacta accagaatcc ttcaagacaa tttcagcagt
     3181 tgaacgagat cggaatggat attatatatg aagagaaagt ttcaggagca acaaaggatc
     3241 gcgagcaact tcaaaaagtg ttagacgatt tacaggaaga tgacatcatt tatgttacag
     3301 acttaactcg aatcactcgt agtacacaag atctatttga attaatcgat aacatacgag
     3361 ataaaaaggc aagtttaaaa tcactaaaag atacatggct tgatttatca gaagataatc
     3421 catacagcca attcttaatt actgtaatgg ctggtgttaa ccaattagag cgagatctta
     3481 ttcggatgag acaacgtgaa gggattgaat tggctaagaa agaaggaaag tttaaaggtc
     3541 gattaaagaa gtatcataaa aatcacgcag gaatgaatta tgcggtaaag ctatataaag
     3601 aaggaaatat gactgtaaat caaatttgtg aaattactaa tgtatctagg gcttcattat
     3661 acaggaaatt atcagaagtg aataattagc cattctgtat tccgctaatg ggcaatattt
     3721 ttaaagaaga aaaggaaact ataaaatatt aacagcctcc tagcgatgcc gaaaagccct
     3781 ttgataaaaa aagaatcatc atcttaagaa attcttagtc atttattatg taaatgctta
     3841 taaattcggc cctataatct gataaattat taagggcaaa cttatgtgaa agggtgataa
     3901 ctatgagcga taaaatactt attgtggatg atgaacatga aattgccgat ttggttgaat
     3961 tatacttaaa aaacgagaat tatacggttt tcaaatacta taccgccaaa gaagcattgg
     4021 aatgtataga caagtctgag attgaccttg ccatattgga catcatgctt cccggcacaa
     4081 gcggccttac tatctgtcaa aaaataaggg acaagcacac ctatccgatt atcatgctga
     4141 ccgggaaaga tacagaggta gataaaatta cagggttaac aatcggcgcg gatgattata
     4201 taacgaagcc ctttcgccca ctggagttaa ttgctcgggt aaaggcccag ttgcgccgat
     4261 acaaaaaatt cagtggagta aaggagcaga acgaaaatgt tatcgtccac tccggccttg
     4321 tcattaatgt taacacccat gagtgttatc tgaacgagaa gcagttatcc cttactccca
     4381 ccgagttttc aatactgcga atcctctgtg aaaacaaggg gaatgtggtt agctccgagc
     4441 tgctatttca tgagatatgg ggcgacgaat atttcagcaa gagcaacaac accatcaccg
     4501 tgcatatccg gcatttgcgc gaaaaaatga acgacaccat tgataatccg aaatatataa
     4561 aaacggtatg gggggttggt tataaaattg aaaaataaaa aaaacgacta ttccaaacta
     4621 gaacgaaaac tttacatgta tatcgttgca attgttgtgg tagcaattgt attcgtgttg
     4681 tatattcgtt caatgatccg agggaaactt ggggattgga tcttaagtat tttggaaaac
     4741 aaatatgact taaatcacct ggacgcgatg aaattatatc aatattccat acggaacaat
     4801 atagatatct ttatttatgt ggcgattgtc attagtattc ttattctatg tcgcgtcatg
     4861 ctttcaaaat tcgcaaaata ctttgacgag ataaataccg gcattgatgt acttattcag
     4921 aacgaagata aacaaattga gctttctgcg gaaatggatg ttatggaaca aaagctcaac
     4981 acattaaaac ggactctgga aaagcgagag caggatgcaa agctggccga acaaagaaaa
     5041 aatgacgttg ttatgtactt ggcgcacgat attaaaacgc cccttacatc cattatcggt
     5101 tatttgagcc tgcttgacga ggctccagac atgccggtag atcaaaaggc aaagtatgtg
     5161 catatcacgt tggacaaagc gtatcgactc gaacagctaa tcgacgagtt ttttgagatt
     5221 acacggtata acctacaaac gataacgcta acaaaaacgc acatagacct atactatatg
     5281 ctggtgcaga tgaccgatga attttatcct cagctttccg cacatggaaa acaggcggtt
     5341 attcacgccc ccgaggatct gaccgtgtcc ggcgaccctg ataaactcgc gagagtcttt
     5401 aacaacattt tgaaaaacgc cgctgcatac agtgaggata acagcatcat tgacattacc
     5461 gcgggcctct ccggggatgt ggtgtcaatc gaattcaaga acactggaag catcccaaaa
     5521 gataagctag ctgccatatt tgaaaagttc tataggctgg acaatgctcg ttcttccgat
     5581 acgggtggcg cgggacttgg attggcgatt gcaaaagaaa ttattgttca gcatggaggg
     5641 cagatttacg cggaaagcaa tgataactat acgacgttta gggtagagct tc
//
LOCUS       DACGLW010000125         5584 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.133, whole genome shotgun sequence.
ACCESSION   DACGLW010000125 DACGLW010000000
VERSION     DACGLW010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5584
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.133"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            complement(487..1296)
                     /locus_tag="IXT53_002736"
     CDS             complement(487..1296)
                     /locus_tag="IXT53_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HAQ4906500.1"
                     /translation="MVKESVIGKKELRSIFWRSFFMQSSWSFDKMMAYGQMNGIERSL
                     RKIYPNDDDYYTALQRHTETFNITPHIAPFVMSLSVTMEEENAKNPEFDTDAINKIKT
                     GLMGPLSGIGDSFFWGTFRVIAAGIGISFAQQGNILGMILYFTLYTAIHFIFKWLATK
                     YGYVLGTKFIDDTEDNHMIDKLSHGASILGLTVIGAMIGTMVTLNTSLKFTVGGAETS
                     LQSIFDQIFPGLLPLGITFLCVWLYSKGHKTITIILGIFVLCFLGTLGGIF"
     gene            complement(1296..2042)
                     /locus_tag="IXT53_002737"
     CDS             complement(1296..2042)
                     /locus_tag="IXT53_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HAQ4906501.1"
                     /translation="MIQAILIALVAFLASIDEYTFGASMMGRPLFTAPFVGLILGDLE
                     SGIIIGATLESMFMGSIMVGSATPPEVYASSVLGTAIAIQSGQGVGTAVALTLPLSIF
                     LQMWRNFAYAMPITYFNNKMDEALVNHDFKKAQKYHYVAQPLIVGVPSAILVFVATYF
                     GVSTINAMIKLIPQVVLDSFDVAAGVLSCVGLALLIKMMANKAMMPYLFLGFVGVMYL
                     KMDVVGVAVVGLCLALLAVQKLTFEEEEDL"
     gene            complement(2060..2527)
                     /locus_tag="IXT53_002738"
     CDS             complement(2060..2527)
                     /locus_tag="IXT53_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HAQ4906502.1"
                     /translation="MIKLLRVDERFVHGQVAFAWTNNLGANCIFVASNEIATNNLRKK
                     TLKMAVPTGTKFVVKDMTEAISVLNGEKIKKYQVFLIVGTVEDAYEITQHVPEIKEVN
                     LGNLKKNENRKMITSSVYLNEQELDLVKKMTENGITVQCQAVPTDKKINPLHD"
     gene            complement(2527..2937)
                     /locus_tag="IXT53_002739"
     CDS             complement(2527..2937)
                     /locus_tag="IXT53_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HAQ4906503.1"
                     /translation="MRKIVIATHSMMAKGLKDTLEFIIGPQKSLLAITAYIDSDYSIN
                     QEIEQLFSDLAPDDELIVTVDLLGGSVSNAFSEQIGKDNFYLISGVNLPLLLELCLSG
                     EQDTKKLIQKAMNSGNEGIVLVNELLEKTEEDDF"
     gene            complement(2948..3961)
                     /locus_tag="IXT53_002740"
     CDS             complement(2948..3961)
                     /locus_tag="IXT53_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009141002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ4906504.1"
                     /translation="MLGFSEERFLSVLNGAVKNIETINQVADAISEKGFKNLYLVGSG
                     GTYATISPLAYLLSTHSELDYYHEIAAEVVCSQPKKINKDTVLITSSLTGTTQETVDV
                     VKYANSQGATTIAFVGNAATPLGEIVDYAIENDADNDTLVEELQLQFFALGARLMKNN
                     GEFPEYDEFIQTLQKMPEVLLSVRQQNDQKALEFAEKHKNTEFHMFVGAGNTYGAAYE
                     YAMCVLEEMQWIATKSIHAAEFFHGTIEMTDKSMSFVLLKGEDQTRPLVDRVERFVNK
                     YSDVVTVFDTADFELAGVPSAMRQYVAPMVMATALERVSAHFEKVRDHSLDIRRYYRT
                     VEY"
     gene            4178..4912
                     /locus_tag="IXT53_002741"
     CDS             4178..4912
                     /locus_tag="IXT53_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ4906505.1"
                     /translation="MNTEELTNKLIQDIEQEKYKYAQAIPSERDFVQIYEMNRSAVKK
                     AIQQLVNKEYLLKIHGKGTFVKKNIHEHLQIKFHGLSELLEREGIQPSSLILASEIKP
                     AGHIVGKALQLEPTEEVFHLVRLRSGDGEPISIEDTYIPLNLIPDLQAFDFQIFSFYS
                     TLAAYHYQIENIYQTISSSKVSNKEARLLKTSLNRPVISLAITAVTTNEQAVEYTEVV
                     VLPEFAAIYTKNVHQGKEMKIYAQID"
     gene            4896..>5584
                     /locus_tag="IXT53_002742"
     CDS             4896..>5584
                     /locus_tag="IXT53_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ4906506.1"
                     /translation="MPKLINLLAYDLIDKIIAYIHEHHLTPGTKLPSERDLSEMFNVN
                     RTTLRGALQKLVDNGTLRAQTNSGYYLTAPKFEKNAHHFFTPIEDSLLSKQRLQLKAT
                     ALSPKKAAALCPELNDFKQQQPQAYLEYIDAEAISLMLLIPMETLKKSNFSDNPFQDF
                     KSLAYTRSQEISLITDNDVAADLLGVDKEEVLLFVREEIRAGKELAAIALGYTISRRC
                     NIQVNVATTLQS"
BASE COUNT         1789 a         1184 c          920 g         1691 t
ORIGIN      
        1 agcgacaaaa accacgagca ggtccattca tcctcaaacc tcggtgatct tcgcggttta
       61 acaccatcag aagaaatgca caaaaataat aaggagagtc tgagacataa ataaattccc
      121 agacttttag tgagtctaaa tccgaataaa cggtgggtcg aaagcaactc tagctgcgcg
      181 tcgatcgcag caatctctag gtgctatgtt cggataaatg gtggtcaaaa gtcagatttt
      241 cggccatttc gtaacagcca accagacaaa aagcgtctgt tttgtcttct actccaattg
      301 ctcaaatctt agcgactttt ggcttggtct tactctaaac agtttgtaag cctaaacttt
      361 atttgctgtt tgtttctaag atcaaagcaa aactactttt tagttggtta cttttacaag
      421 ttctttttac tgttgaataa atgtaaatct taagaacaaa tatttacttc tataattcca
      481 agtttcttaa aaaattccgc caagtgtacc taaaaagcat aaaacaaaaa ttcctagaat
      541 aattgtgatc gttttatgcc ctttggagta aagccagacg cataaaaagg taattcctaa
      601 tggcagcaag cccggaaaaa tttgatcaaa aatactttgt aatgaagttt cagctccacc
      661 aaccgtgaat tttagtgacg tatttaaagt aaccatcgtg cctatcatcg cgccaattac
      721 cgttaaacct aaaatcgaag caccatgaga taatttgtca atcatatggt tgtcttctgt
      781 atcatcaata aatttagttc ctaaaacata gccatatttt gtcgccaacc atttaaaaat
      841 aaaatgaatg gcagtataca aagtaaaata aaggatcatg cctaaaatat tcccttgttg
      901 agcaaaagaa atacctatgc cagccgcaat aacccgaaaa gttccccaga aaaatgaatc
      961 gccaatacct gataaaggtc ccattaatcc tgttttgatt ttattaattg cgtctgtatc
     1021 aaattcagga tttttagcat tttcttcttc catggtgacg gacaagctca taacaaaagg
     1081 cgcaatatgc ggtgtaatgt taaatgtttc cgtatggcgc tgcaacgctg tataataatc
     1141 atcatcgttc ggatatattt tacgtaagga acgttcaata ccattcattt gtccatatgc
     1201 catcatttta tcgaaggacc aagaggattg cataaaaaag gagcgccaaa atatacttct
     1261 taattctttt ttcccaataa cactttcttt taccattata aatcttcctc ctcttcaaaa
     1321 gttagctttt gaacagccag taacgctaag caaagtccga cgacagccac accgacaaca
     1381 tccattttca gatacattac gccgacaaat cctaagaaaa gataaggcat catcgcttta
     1441 ttcgccatca ttttaattaa taacgctagc ccaacacaag aaagcacacc ggccgcaaca
     1501 tcaaagctgt ccaatacaac ctgaggaatc aacttgatca tcgcattgat tgtagacaca
     1561 ccaaaataag tagctacaaa aactaaaatg gccgatggaa ccccaacgat taaaggttga
     1621 gccacataat gatatttttg tgcttttttg aaatcatgat tgactaatgc ctcatccatt
     1681 ttgttgttaa aatacgtaat tggcattgca taggcgaagt tacgccacat ctgaagaaag
     1741 atggacaaag gtaaagtcaa cgcaacggca gttcctactc cttgacccga ttgaatcgca
     1801 attgctgtac ctaaaacact ggaagcataa acttcagggg gcgtcgcact ccctaccata
     1861 attgagccca tgaacatgga ttccaaggtg gcaccaataa taattcctga ctctaaatct
     1921 cccaaaatca aaccgacaaa aggcgctgta aatagcggtc tacccatcat cgatgcacca
     1981 aatgtgtatt catcaataga cgccaaaaaa gctaccagtg cgattaatat tgcttgtatc
     2041 atattcttcc cctcctcttt tagtcatgca aaggattgat ttttttgtcc gttggtacag
     2101 cctgacattg aactgttatc ccgttttcag tcattttttt caccaagtcc agttcttgtt
     2161 cattcaagta aacggaacta gtaatcattt tccgattctc gtttttcttt aaatttccta
     2221 aattcacttc tttaatctca ggtacgtgct gagtaatttc gtaggcatcc tccaccgtcc
     2281 cgacaattaa aaatacttgg tatttcttga ttttttctcc atttaaaaca gagatggcct
     2341 ctgtcatatc cttcaccaca aattttgtcc ctgtaggaac agccattttt agtgtttttt
     2401 tgcgcagatt attggtagcg atttcattac ttgcaacgaa aatacaattt gcacctaagt
     2461 tatttgtcca ggcaaaagca acttgcccat gaacaaaacg ttcatctact cgtaataatt
     2521 taatcattaa aaatcatcct cttctgtttt ctctagcaac tcgttcacca aaactatccc
     2581 ttcattcccg ctattcatag ctttttggat tagttttttc gtgtcctgtt cacctgataa
     2641 gcaaagctct aaaagcagcg gcagatttac accgctgatt aaataaaaat tgtctttacc
     2701 gatttgttcg gagaaagcgt tagacacgct tcctcccagt aaatctaccg tcacaattaa
     2761 ttcgtcatca ggcgctaagt cggaaaatag ctgctctatt tcctggttaa tggagtagtc
     2821 actatctata tacgcagtta ttgctaaaag tgatttttga ggaccaataa taaattctaa
     2881 agtatccttt aagcctttag ccatcatgga atgcgtcgca attacaattt tcctcatagc
     2941 tcttacctta atattccaca gtccgataat aacgacgaat atctagtgaa tgatcccgca
     3001 ccttttcaaa atgagcactc actcgttcta aggccgttgc cataaccatt ggcgctacat
     3061 actgacgcat agcgcttggt acacctgcta attcgaaatc cgcagtgtca aaaaccgtca
     3121 caacatcgct atatttattt acaaaacgct ctacgcgatc gactagcgga cgcgtttgat
     3181 cttcaccttt taataacaca aaactcatag atttatcagt catttcaata gtcccatgga
     3241 aaaattcggc tgcatggatc gacttggtgg caatccattg catttcttcc agtacgcaca
     3301 tagcatattc ataggcagcc ccataagtat tcccggctcc aacaaacata tgaaattccg
     3361 tatttttatg tttttctgca aattcaagtg ctttctgatc attttgttgc cgaacactta
     3421 gtaatacttc cggcattttt tgtaatgttt gaataaattc atcatattca ggaaactcac
     3481 cgttattttt catcaggcgt gcacccaaag caaaaaactg taactgaagt tcttctacta
     3541 atgtatcgtt gtcagcatca ttttcaatcg cataatccac gatttccccg agtggtgtag
     3601 cagcatttcc tacaaacgca atcgtcgttg caccttgact attggcatac ttaacaacat
     3661 caacggtttc ttgagtcgta ccggttaatg aagatgtgat taacactgta tctttattaa
     3721 tctttttcgg ctgtgaacaa accacctctg cggctatttc atgataataa tcaagctctg
     3781 aatgagtgct taacagataa gctagcgggc tgattgtggc atatgttcca ccagagccta
     3841 ccaaataaag atttttaaat cccttctcac tgattgcatc tgccacctga ttgattgtct
     3901 cgatattttt tactgcacca tttaatactg atagaaaacg ttcttcgcta aaacctaaca
     3961 tagttaaaaa cctccaagtc aataatttga aaaatatatt tgcaaatgcc ggcacattaa
     4021 caaaccactt aacctttttt atataccacc tgttaacgtt tttaatgtac cacttataga
     4081 aaacgctgtc aatagttttt aaccactttt atttttattt gtaccaaaat ccttttttca
     4141 tgtataataa acaaaaatag actctggagt gtaacgtatg aatactgaag aactaacgaa
     4201 caaattaata caagacattg aacaagaaaa atataaatac gcgcaagcta ttccttcaga
     4261 acgtgatttt gttcaaattt atgaaatgaa tcgttcagcc gtaaaaaaag ccattcaaca
     4321 attagtaaat aaagagtacc tactaaaaat acatggcaaa ggaacttttg ttaaaaaaaa
     4381 tatccatgaa catttgcaaa tcaaatttca tggtttgtct gaattgttgg aacgggaagg
     4441 gattcagcct agcagcttaa ttttagcttc agaaataaaa ccggctggac acattgtcgg
     4501 gaaagcttta cagctggaac caactgaaga agtatttcat ttggttcgtt taagaagcgg
     4561 cgacggtgag ccaatttcta ttgaagacac ttatattcca ttaaatctca tacctgatct
     4621 tcaagctttt gatttccaaa ttttttcatt ttattccaca ttggctgctt atcattatca
     4681 aattgaaaat atctatcaga caatttccag ctcaaaagtg agcaataaag aagcccgttt
     4741 gttaaaaacc agcttaaatc gtccggtaat cagccttgct atcactgcgg ttacgacaaa
     4801 tgaacaagcg gttgaataca ctgaagtagt ggttttgcct gaattcgccg ccatctatac
     4861 taaaaatgtt catcaaggaa aggagatgaa aatctatgcc caaattgatt aatctgttgg
     4921 cttatgacct gattgacaaa attattgctt atattcatga acatcattta acccccggaa
     4981 caaaactgcc ttcggaacgg gacctatcag aaatgtttaa tgttaatcga actaccttaa
     5041 gaggcgcgtt acaaaaactg gtggacaacg gcaccttacg ggcgcaaacc aatagcggct
     5101 attatttaac tgcgccaaaa ttcgaaaaaa atgcccatca tttctttaca ccgatagaag
     5161 attctctgct tagtaagcag cggctacagc taaaggcaac ggctctttca ccgaaaaaag
     5221 cagcagctct ttgtccggaa ctgaatgatt tcaaacagca gcaaccacaa gcttatttag
     5281 aatatattga tgcagaagca attagcttga tgttattaat ccctatggaa acattaaaga
     5341 aaagtaattt ctcggacaat cctttccaag attttaaaag tttggcatat acgcgttctc
     5401 aagaaatttc tttaataact gacaatgacg ttgcggcgga cttgctggga gttgacaaag
     5461 aagaggttct gttatttgta cgagaggaaa taagagccgg taaggagttg gcggcgattg
     5521 ctttaggtta tactattagt cgccgctgca acatacaggt taatgttgct acgaccttgc
     5581 aatc
//
LOCUS       DACGLW010000126         5507 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.134, whole genome shotgun sequence.
ACCESSION   DACGLW010000126 DACGLW010000000
VERSION     DACGLW010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5507
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.134"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            142..1527
                     /locus_tag="IXT53_002743"
     CDS             142..1527
                     /locus_tag="IXT53_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HAQ4906507.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            1564..2241
                     /locus_tag="IXT53_002744"
     CDS             1564..2241
                     /locus_tag="IXT53_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HAQ4906508.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            2249..3013
                     /locus_tag="IXT53_002745"
     CDS             2249..3013
                     /locus_tag="IXT53_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HAQ4906509.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            3015..3674
                     /locus_tag="IXT53_002746"
     CDS             3015..3674
                     /locus_tag="IXT53_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HAQ4906510.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(3704..4213)
                     /locus_tag="IXT53_002747"
     CDS             complement(3704..4213)
                     /locus_tag="IXT53_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HAQ4906511.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(4325..5323)
                     /locus_tag="IXT53_002748"
     CDS             complement(4325..5323)
                     /locus_tag="IXT53_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HAQ4906512.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
BASE COUNT         1813 a          970 c         1090 g         1634 t
ORIGIN      
        1 attttttagc cagactgatt tatcgaatat tgtacttata caatttgctt caaggcagga
       61 aaaactctga aatagagctt gtttcaatgg aagaaaaagc tgcctatggt atgataaaga
      121 taaagagagg cgagagggta catggaaaaa atagtgattt tacatacgaa tgatctgcat
      181 tctcatttag aaaattggcc aaggatcaga aggtttttgg atcaacgtaa acgagaaaac
      241 gaaaagaaag aaaaaacgac aacgattgca gtagatctag gtgattttgt ggatcgttgg
      301 catccattgt cagaagcaac gaatggacag gaaaatatct tgctgatgaa tgaagttggt
      361 tatgatgcag cgacgatagg aaacaatgaa ggcgtaggaa attccaaaga agagttgaat
      421 catttatacg atcatgctga gtttgatatc atcctggata acttattcga taaaaatacg
      481 ctccagccac cggcatggac gaaaccatat aaaatcatta caacgaaaca ccagacgaaa
      541 atcggactaa ttgcatttac tgctccattt ccgcttactt ataatccgaa tggctgggac
      601 atacggaatc cttatgatat tttgccagaa ttagtcgaag atttacgttc acaagtggat
      661 gttttagttt tgatgagtca tctcgggatt caggatgatt tccaaattgc aaaagaaata
      721 ccagcaattg atgttatttt aggatcgcac acccatcatt tattcagaga aggtcacgta
      781 atcaatgatg tccaaatagc agccgctgga aagtacgggc agtatgtcgg tgaagtccat
      841 ttaaccatcg atgaagagaa aaaaatcctt aagcattcag ccaaagccat ccctaccgaa
      901 acaatgacag cttttacaga agatgaacaa gaaataaata gttatctaga acgtgggcat
      961 gaattattaa aagaaaaaga agtagcagat ttgccatttt ctctgagtct agatatcttc
     1021 aatgagcatt cgtttatcca agtagccttt gaggcggtga aagaacgagg aaagacggaa
     1081 gcagcaatat taaacagtgg tctgttttta actgaaattc caaaagggcg ggtcaatcag
     1141 gatcagcttc acactgcgtt gccgcatccg atgcacttgt taaatgtcac attgggtgga
     1201 aatgatctga tccgcttagt tttagaaata gaaaaaaatc gtaacttttt acgtaactat
     1261 cctatgaagg gaatgggatt tcgcggtaag atttttgggc agatggtcta taatggaatc
     1321 acatatgatt ctattaacca tcaagtcttt tggaagaaca agccaatcga tctgaataaa
     1381 aattatactt ttactaccgt agatcatttg atgtttgttc cattttttcc aacgattgag
     1441 attgctggaa aaaatgaatt tttgttccct gaatttatcc gaagtgtggt aggagattac
     1501 ttgaaagctc actacccaat caaataaaaa caaggtataa taaaaaagat aggtggtgaa
     1561 cgcatgtctg caaaacaaaa tgaacagcag aacgttgagc ataaaacaga aattgctaag
     1621 aaaaaatcat cagctgaaca ggaaacaaca gtcaccgaag aactgacagc tgctttggaa
     1681 gaaatcgttg aggagcctaa agcagaaaaa gtcaaaggcg agctagtgac actgttagat
     1741 gacagtaatt ttttgatagg tgaaagacat tatcggcttg tgtccgatta tcgtgaagga
     1801 tttaatgcag aaaagcttgg agaacgttac agtgatgtac tagcccgata tgattatatc
     1861 gtaggagatt ggggttatga acaactacgc ctaaaaggat ttttccaagc ggataatcga
     1921 cgggcgcatc cagatcagcg aatcgattcc ttggaagact atctttacga atattgcaat
     1981 tttggttgtg cgtattttgt catcgagaga atcggcggta aaaaagaaaa gacaactcag
     2041 cgccgaaaaa agaaaaaaaa ccataatcgt gcgtatatcg atgagaaaaa aggaccagtc
     2101 gcatctaatc gcaaaaaacc ggttattaaa aatcgaaaaa cagagtcagc aaaaacctct
     2161 gaaaaagtaa aaaaacaaga acaacctaaa caagtagaaa aaacaaaaac agcatttacg
     2221 atcagaaaaa gggaagagta gtggatgaat ggactataaa ggatatttaa ttgatttaga
     2281 cggaacgatt tatcgtggga cacaacccat accggccggt aaacgatttg tggaagaatt
     2341 gcaaaaaaga aaattacctt tcttgtttgt taccaataac acgactaagt caccagaaac
     2401 agtagctaat cgtctagcag atgagtttga cattcatgta gctccagaga cagtctacac
     2461 agctactttg gcaacaatcg attttatgaa agcagatgga aaagggaaaa aagtctatgt
     2521 tatcggagaa gcaggattga ttgacttgat tttagctgcc ggatttacat gggaagaaga
     2581 ggctcctgac tatgtagtgg taggcttaga taattatctg acctatgaaa aagtagttaa
     2641 agcaacactt gctattcaaa aaggtgctac tttcattgga acgaacccag ataagaatat
     2701 acctactgaa cgcggccttc ttcctggagc agggtcagtc atctcttttg tagagacagc
     2761 aacccaaacg ccgccgatct acatcggaaa accagaagcc atcattatgg ataaagccgt
     2821 tgaagtatta gggctgcaaa aagaagaagt aatcatggtt ggagataact atgaaacaga
     2881 tatccaagct gggatacgca ataatataga tactttgctc gttttatcag gttttacaaa
     2941 agaagaagat gtacctgggt tgcctatccc agctacctat gtgaaacaat cactggatga
     3001 atggagcttc taatatgaaa aattcgagat ggcagtggat ggaatatgct ggattgttca
     3061 gcctatttct gacactgatc agtctggcag ttggtgtgac gatcaacttt cgaccactat
     3121 acgtatttga tatcggacat ttgcaaattt tggattatac ttctttagat caagagacgt
     3181 tactgaaaaa ttttgatcac ctaatgaatt atctcaataa tcccttcaaa accatcctca
     3241 gcttgccaga tttcccagtt tctgctagcg gagcccatca cttttatgaa gtgaaaattt
     3301 tgtttttagt ggattatgct gtgtttttca tcactttgat cccgagtatt ttgtttatca
     3361 aatatttgca aaagaatgat cggctttggc ggttgattcg tccgtttcaa atcggcatgt
     3421 tactgccagt tgttttcggt tttttcatga tgataggatt tgatcgcttt tttatccttt
     3481 ttcatgaaac gtttttcaac aacgatgact ggttattcga tcccgttact gatccaatca
     3541 tcaatgtact tccagaagaa tttttcatgc atagctttat cttatttttt gtattattag
     3601 aattattttt tgctgttttt cttttcttag gaaaaaatag cttgaaacaa acaaaaaaga
     3661 aagaactcgt ctaaatagac gttttctttc tttttttgtt gaattattga gtaaggcgac
     3721 ttcttttttc tggttctttg tgtgctaatc gactggcagc agcagcggcg atcgctccga
     3781 cgatatcatc taagaaggta tggacatgaa cgccatcgtg gctgtttagt tcttttaaaa
     3841 taccaggttt caccttgtcg atataaccat aattcgtaaa gccgatcgta ccgtaaacat
     3901 tcacgattga caaggctagg atctcatcga taccgtaaag gccttcgtca tcttcgataa
     3961 tatgttgcaa cggactcatc aattttttct tttctgctaa tatatctaat tggattccag
     4021 tgataatggc gttatgtact tcacgctttg ttagcacgct gttgacactt tcagtacaat
     4081 ccgccaaagt caaatcagaa atatagtctt tttgtaaaaa catgacaagt tgtgcgatat
     4141 cttctacagt tacgccacgt tccttcaata gttctcttgc cttttcttct aaagtctcag
     4201 ttttgacgac catattattg ctcccccttt attttttctt tctctatttc atgttaacgg
     4261 aacatgaaaa aaatgcaaga aactgctttc gggtttaact tgaaatgcaa agagatataa
     4321 aaaatcaact aggacgaaga tttttatcgt ataagcttgt gctgtgacca ggctgagttc
     4381 gttcctttgg attaatatag tgtaaagcgt tgttcactgc agtaggtgct tcaccgagcc
     4441 cagtagcaat cagtttcact tttccttcat aggtacagat gtcaccagct gcataaacgc
     4501 ctggaataga agtagacata tctgatttta cagtaatggc atttctggta ctatcaagtc
     4561 cccaagaaga aagatgttct aaattagaag agaatccata attgacgatc agagaatcaa
     4621 tcatcaaatc aatcgtctcg tcacttttca ctttttttaa ccggatatct gtcagttccc
     4681 cattattgcc tgataatcca tcgatgagat agggagtaag caagttgaca gatgaagatt
     4741 tcaagcgtga tacactatgt tcgtgcgcac gaaattccgg gcgacgatga atcaagtaaa
     4801 cttcgcttgc aataggctct aacatcaacg tccaatcgat agcagaatca ccacctccag
     4861 caatagcgac tttttttcca gcatacttca tcaaatcatt cacgaaataa tccaatccgt
     4921 ggttttcaaa tgattcagca ttatctaaat tcaattttct tggctggaat gaaccatttc
     4981 ctaaagctaa aatgactgct ttggaataat ggatgccttt atttgtagta atctcgataa
     5041 cttcgtcttc acgagtaagc gagagaactt cttcttttaa atggaatgta tggttaaaag
     5101 tagttaattg tttttctaat tgatcgatta attcacttgc ttttattgct ggatacccag
     5161 gaatatcgta aatatatttt tcgggataca aagttgctaa ttgaccacca agttgaggta
     5221 gactgtcaat gatttttgtt ttagcttggc gcattcctgc gtaaaaagcg gcaaacatcc
     5281 ctacaggacc tgcaccgatg attgtaatgt catagatttc cataaaaatt cctccctttt
     5341 cacactatag cagaatttaa ttgagaatgc ttctcaaaaa tgtttgaatt cgaaatcgaa
     5401 acggtaagtt atagaatgta tctagtgatg aaaaataaaa gaaaacaaat ctagtatttt
     5461 tacgagaggt taggtattat gttattatta ttatgaagta tgcaaat
//
LOCUS       DACGLW010000127         5450 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.135, whole genome shotgun sequence.
ACCESSION   DACGLW010000127 DACGLW010000000
VERSION     DACGLW010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5450
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.135"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            <1..108
                     /locus_tag="IXT53_002749"
     CDS             <1..108
                     /locus_tag="IXT53_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HAQ4906513.1"
                     /translation="KCIKSYRLYQCPTCGKKYTLSYGDYILIDEKGKKR"
     gene            108..359
                     /locus_tag="IXT53_002750"
     CDS             108..359
                     /locus_tag="IXT53_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906514.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            374..811
                     /locus_tag="IXT53_002751"
     CDS             374..811
                     /locus_tag="IXT53_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906515.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFESIEKNGAFYTIESEEKDNE"
     gene            804..1511
                     /locus_tag="IXT53_002752"
     CDS             804..1511
                     /locus_tag="IXT53_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906516.1"
                     /translation="MNKVVYQLTTEDVSQFLTSKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNHLDVYKRRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGK"
     gene            1892..2071
                     /locus_tag="IXT53_002753"
     CDS             1892..2071
                     /locus_tag="IXT53_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906517.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            2412..2675
                     /locus_tag="IXT53_002754"
     CDS             2412..2675
                     /locus_tag="IXT53_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HAQ4906518.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            2669..3019
                     /locus_tag="IXT53_002755"
     CDS             2669..3019
                     /locus_tag="IXT53_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HAQ4906519.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(3043..3657)
                     /locus_tag="IXT53_002756"
     CDS             complement(3043..3657)
                     /locus_tag="IXT53_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ4906520.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRL
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
     gene            3971..4393
                     /locus_tag="IXT53_002757"
     CDS             3971..4393
                     /locus_tag="IXT53_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906521.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            4403..4606
                     /locus_tag="IXT53_002758"
     CDS             4403..4606
                     /locus_tag="IXT53_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906522.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            4817..5401
                     /locus_tag="IXT53_002759"
     CDS             4817..5401
                     /locus_tag="IXT53_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ4906523.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT         1940 a          822 c         1036 g         1652 t
ORIGIN      
        1 aaatgtatca aaagctatcg tttgtatcaa tgtccaacat gcgggaaaaa atataccttg
       61 tcttatggcg attacatttt gattgatgag aaaggaaaga aacgctgatg aaaacacaac
      121 acaaattatt gactgtaaaa gaagtcgttc aaattttggt ggacaaagaa gtcatttctg
      181 aaaaagtagc agaagcttta cttattccgc ggtactggac gatagaaacc cctttagtag
      241 aggatgcacc gactatgcta tttgaagacc tagtgaagat tgcaggatat gaactgcatt
      301 atcaagaaaa catggaaact tatgaaatcg aatattggtt aaccaaatta gagaactaaa
      361 aaggagaaaa aatatggctc aaaattacta tgacgaattt gtcaaattac ctttggataa
      421 aatggcacaa aaaatggagg atatgacgtt cctataccat gaaacacgtg tacccaaaaa
      481 acattacaag gaaaaattgt cagtcgcagt ggaagaaatg attgaatcag gtgtcgaaat
      541 gaatctcatt gcgacgtact atcgaacact ggaagaactg aaaaaacaaa atggaaaatg
      601 gtttttccaa gccttactat gcttggaagt aggtgtcaaa ccaagcacta tcaaaccttc
      661 cgaatatcaa gccttagaac tgacttatgc aaaattcgca cagattaaga aagctaaagc
      721 aatggctagc gactggctag atacttttga aagtatcgaa aaaaatggtg cattttatac
      781 gattgaatcg gaggagaaag ataatgaata aggtggttta tcagctaaca acagaagatg
      841 tttcgcaatt tttaacaagc aaagaacact tgaatgaagt aatcagacgg attcaaaaag
      901 actttagttg tatggaagaa atgaagtact ttattcaaag agtggaagaa cagttgtata
      961 tggaaagcga aacaagtcaa acgaaggtca cagtcagcca taatcattta gacgtgtata
     1021 aacgtcgtcc tgaaacggta aaaaaaggaa cagagcttct acgactagaa acagaaaaat
     1081 acatcatgaa tgtgtaccta gaaaggagtg agctggtcga aaactttgtc agctacacga
     1141 tttgtaggaa acactattac tcagaatcag aatattattc aattttcgag agcatggaat
     1201 taatggaaga agaaatggaa gagctaaaca gcaaaaatgc gacggtagac gattattacc
     1261 ttgtatttac taggtttttg acgtgtggga cggaatctat cgtgttaatg ggaatggatt
     1321 atgaactcgc tgtacccgaa gcatttttga gggaatatgt agaagaagta gataacccag
     1381 gcgacgactg gtacacgact ttaactgatt ttttagacac ctacacttac gacgatacag
     1441 aagaaatcca aaatgaactt gaccgtaaac gaatgccata tcaacgattt gaaaggaaaa
     1501 aaggtaaata accatttata ccgaaaagtg aggatgtgtg aaataagaac aaaaaacctt
     1561 gaaattaagg gaatggttct tatttcgcac caatcgctcc aaccgccccc gccgcttagg
     1621 tggtgcgaaa taagcgggca aagcccgata acctaagaga aagaaggaga tccatatgaa
     1681 taaaatcatt cctctattgt tcgtactgtt tgttggcgag atacttatgc gtcatgctgc
     1741 agctgtacat ttaaacttcg tagtattctt aggaatactg gtattttgtt tgatcttttg
     1801 gcgatttgta ttagcccttg tgagtctact attcaagatg attgcagcca tatttttcat
     1861 agggttattt cgatgaaagg agagagtact aatgccaatt gcaataggaa ataaacggtt
     1921 acccgtgaca ttagatgaaa agagacaaaa agaattgcag caactaaagc agaagtacgg
     1981 aaaaagtgaa tctaagatta tgtgtattgc gttagatcta ttgatcgcgc aagaaaaagc
     2041 aggatttgat ataccagcac taaaaaagtg acgtcacttt tcatttgaag aaacaaaagt
     2101 gatagcactt aattgttcct actatttaga atattttcta tgtccttttt attccatttt
     2161 gtatcaataa ctatgaaatc tttaaagtat ccagaagaga ttatttttag atcaattttt
     2221 ttcttacctg attctaaaga ttttgttttc ttaaacatat tatgatttcc tttcgactat
     2281 tttcatataa atgttaacat agtttatgga ttcatggaat gttaatctaa tttaatagaa
     2341 aaggataatt aagtatatac gttttttctt atgtatgtta tactatgtat atacagaaag
     2401 aggtgtctac tatggaaatg ttacctataa aaaaatgggg aaatagtaat ggccttagat
     2461 tgccaaagta tattatggaa tatcttggaa ttcatacaga agacaaagtg aagataatac
     2521 aagaagaagt aaacggtcag aagcggctaa ttatcgaagc ggctaatata gaaaatgaat
     2581 taacaattga acagttattt gaaaattaca ctgatgaaag aaatcatgtg acgattcaaa
     2641 atttaggtga agcagtaggt aatgaaaaat ggtgagaaaa cctagacaag gcgatattct
     2701 attattgaat acagcacctc gatctggaca cgaacagaca ggtaaaagac cttatattgt
     2761 tttaagtcat gatattattg cggattatag taacgttgtt acagttgctc caatttctag
     2821 tacaactaga aactatccgc tatatgtaaa tataaactca gaatatgaaa tgaagacaac
     2881 aggaaaagta ttattagatc aactaacgac aattgattat gaagccagag agtgtatttt
     2941 tttggaaaaa gcgcatgata gtttagttga ggagttattg atgaaagtta gaacagtttt
     3001 tcaaaaagta agcaaataaa atagaaacag cagcatgatc aactattcgt gattgaccat
     3061 gctgtctttt tttattctat atacggtttg tctcgtaaca ttgtagtctt ttgcaatctt
     3121 agcgatcgca acaccttttt ttagatcctc aacgatgttt ttataaacaa gacgacgttg
     3181 gggatctttt gcattggcac tgtatagctt aggacgtcct ttataaacac cgttcgcttt
     3241 tgcaatctta atgccttgtg cctgacgtcg tttagattcg gtacgttctt gttcagcgat
     3301 catggccaaa atttgaatga tcaaatcttt aatgaattta tccaggagag gattgccaat
     3361 tgcttcggcc atgatcggta gacttgtgat gatcagctgc acatttttct tcttcaagta
     3421 attgactgaa gcgatgatct cgtcgtaatt tcttccaagt cgatcaatag cttccacaat
     3481 aaaaatatct tgatctcgga cgaaatccag tgcttcttga aaaacaagtc gatgagtaat
     3541 cgttgcgcca gattgtttct caatgaaaat tctctctgca ccgaattttt tcatttcttc
     3601 aatctgccgt tgttcatttt gatcgatgga tgagacacgt atgtatgcaa ctctcaaaat
     3661 ttttacctac tttctgcaac tatttaaaaa acaccctaat catacacgag aaaaccagat
     3721 ttttcaatgt cgcggtcgtg tctagttagg gtatacctta tatagacaaa aactaatgat
     3781 ggaggaaata gtatgtgtca cttttctttt taaatggcaa tattttttgt aaataaatgt
     3841 caatcaagaa aatttaaacg attatgacat taaaaatata ttttatgaat tttctagtat
     3901 ataacttgat ttatctgtta agatttatta agaaaacgtt ttcttatttt tgatttcgag
     3961 gtgatagaca atgaaaaata agtttatttt atctttaggt attttaacta ttggaattag
     4021 tgtaactgga actacaattc ctacatttgc tgatagtaca aatgaaccaa ctactgtagt
     4081 agataataac caagaattaa ctcctttaga taggaacgga atagccattg gtaacccaac
     4141 taacgactta actgttactg aaaattattt aatagtttct ccattacttg ctcgagcagc
     4201 ttctaaaacg atacgagtga caggaacgat gatttttgat aaagttggtt ggccttctag
     4261 acctcctcaa tcacggaatt ggaaagaaaa aagaaatggt tattggtact atggaaaatt
     4321 gtatttacaa agttataaat cagctggaaa caattggatt gcgacttatt caggaacctt
     4381 aactagaaag tagggatttt ttatgccaga atttgtgaac acaccaaatt tttatcaaga
     4441 tagcgatatt ctaagtaaaa ttaatttacc gaaagaagaa tcagctttac aagttcagcg
     4501 aactttacag gagacacaaa aaattcaaaa atcaatttct aatctagaag attctgaaga
     4561 aaaaagaaaa aaaagagacc aaaaaacata tcaaaaagaa aaataatgat tttaaaaaaa
     4621 gagtgtctag tcgacattct ttttttctaa ttgaaagtgt tcataaaatc acccatttct
     4681 ggacactttc tttttttgct ttatttttca aaaaaacaat tcatttcatg ttcataaacc
     4741 cataacaata tctatgttca gttaaactat atggataaag ttatgttaca ataaaattaa
     4801 tgaaggagtg atttttttga aaataggata cgcccgggta tctactggtt tacaaaattt
     4861 ggatttacaa gaagacagcc taaataaatt tggttgtgaa aaaattttta ctgatcatat
     4921 gagtggggca aaaagtaatc gaccaggatt agaaatggcc attgattttg ttcgctctgg
     4981 cgatacactt gtcgtttggc gcttagatcg tttaggtaga aatatggaag atttgatttc
     5041 aatcgtcaat cgactgaatg agcgtggtgt gagttttcat agtattcaag aaaatattac
     5101 gatggacaaa tcaagttcta caggacagct catgtttcat ttgtttgcgg catttgcaga
     5161 atttgagcgt aatttgatcc tggaacgatc cgcagcagga cgagcagctg ctcgtgcaag
     5221 aggacggttt ggtggtcgtc ctgaaaaatt gtctgaacaa gatttggaat tactgaagac
     5281 tttagtggat agcggtacac ctattaagac tattgcggaa cggtggaacg tctcaagaac
     5341 caccatttat cgttatttgg caaaaatgga ggagaaaaat ggaaagacaa attctcatta
     5401 gtttttgtca tttaatgctg aaaatttttg ccatttatct agaaaagtga
//
LOCUS       DACGLW010000128         5338 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.136, whole genome shotgun sequence.
ACCESSION   DACGLW010000128 DACGLW010000000
VERSION     DACGLW010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5338
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.136"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            complement(61..279)
                     /locus_tag="IXT53_002760"
                     /pseudo
     CDS             complement(61..279)
                     /locus_tag="IXT53_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(545..739)
                     /locus_tag="IXT53_002761"
     CDS             complement(545..739)
                     /locus_tag="IXT53_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906524.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
     gene            2091..3131
                     /locus_tag="IXT53_002762"
     CDS             2091..3131
                     /locus_tag="IXT53_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein RepA"
                     /protein_id="HAQ4906525.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESDISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYA
                     SSPSTITKDWLE"
     gene            3955..4287
                     /locus_tag="IXT53_002763"
     CDS             3955..4287
                     /locus_tag="IXT53_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HAQ4906526.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            4906..5247
                     /locus_tag="IXT53_002764"
     CDS             4906..5247
                     /locus_tag="IXT53_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HAQ4906527.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT         1942 a          728 c          900 g         1768 t
ORIGIN      
        1 aggtattttt attatatata gatttaacat atgtcggtcg gttgattgat atagctaaag
       61 ttataactca accgaagccg attcatctac acgaataaag ttgcgagtgt tctcggctaa
      121 ttcataaaaa ttgttatgtg ttccatatag caatactgga ataactttat tttcttgaat
      181 ctcataagca actctataca cggtgaccgc atacttaaat ccattagacc aattacctga
      241 taaacctcca gttttttgct cttacttaaa tggatcaatg gcaatttcat cacaaaccta
      301 tagaggatga gtgacttctg cctaattacg ttaaaaaata ttgaaagtaa ctctatgtag
      361 caaaaaatat ctagaatttc atttcctcaa aactgatcga tattgtatct aaaaatatat
      421 actacctaaa ataggtagta tatattaaaa tatgtgcttt tgttagaaaa atcaatacat
      481 aaattatcat atctgtatat gtcatttagg tatttcagtt atgttataat agttttgctg
      541 tctgctaccc cggcctaatc tggcaagggg aggaggagat ataatgaaat acctttttga
      601 ttttgttttt gcaatcaagc cacttgcaga tgtagtgtgc agttacctct gcaaatggct
      661 tgattgcaaa attgccaaaa aagacagtaa gcctacccgt taattctata atatagaaac
      721 gtaaaaaaaa ccccatcagc ctgcacgctg atggggtttg gtgatataat gaaatacctc
      781 tttgttactc ataattatac cataattagt cacgaatgca accaatagaa aaaactactt
      841 agaacggtag atcatcgcta ataacaatgc attgtccacc aaatggattt tgtgaagcat
      901 taaatacatt gttattaatt gtcgctgctt ttaactaggc ctctcccctt ctattataaa
      961 agaagatata tatcatttct taattaccta ctaccttaaa ccaaaattta agtactacct
     1021 aatttaagta gtacttaaac aatctaaaaa atagttatcc caattgttat gcttttataa
     1081 tacgattatt tgtaacaaaa tgaatattat ataattaaac actaccatat ttaggtagtg
     1141 tttaattatt agcaaaacaa aaaatattta ttctattctc ttaacttcaa ctaagttatg
     1201 ctgcacacct ttatataaat catatgcaac tcattagcta gacaaactac aagcatgtag
     1261 ttttatctgt taattatgta ctacctaaat aaagtagttt ataattagca agtatatata
     1321 tttaaaaatt taagattaat ttttttatga cttgtacaaa tttatctgat attctcttat
     1381 taagaactac ctaatttggg tagttcttat tttttacatt ttaagtagta tttaattgca
     1441 atttggtagt atttttttat tatcttattc ttagaacggt agtacttaat ataaatatag
     1501 gtagtatcta atttaagtat taatggtagt atctaaatta acaggtagta tgtaactact
     1561 ttaaagtaat ttttttcaga ttaacacagt agtatcttat taaataagtt aattttaatg
     1621 gtagtacata attattatat atatctgaca ttggtagtac ttattgtatt ttttagtagt
     1681 atataatcta ttgttttcta gcctaaagcc ttgtcttata agaataatat aatatatact
     1741 actgaaataa ggtagtatat attactagta atggtagtat gttttatatg cataaggtag
     1801 ttcttaattt attttttgtt ttgacactgg tagtacttaa ttatctttaa ttttaaaact
     1861 aaattttatt catacgtttt tctggtagta tttaattata aataatattt atttaaagta
     1921 gttcttaatg actttaaagg tagtacataa ttcattttat ttctgatttt aggtagtatt
     1981 taattaaaaa acagttttaa tgcgtttgca ttttttattt atctttgcta ttttcgaaaa
     2041 gagtaggtaa ttttacataa ataaaacaac gtgaaagtag aggatctaca atgactgatt
     2101 ttaaattttt taaggcggat agagtttaca acgaattatt ttatcaattt ccaaaagtct
     2161 ttattgtttc tgacgaatac aaaaaaatga aagattcaac taagattgcc tatatgcttt
     2221 taaaagcaag attagagatc gcaatcagca aacggcaaat cgatgaagaa ggtaatgttt
     2281 attttactta tacgacaaat gaactatgta gagtattaaa ctgccaaaaa caaaaagcga
     2341 tagcaatcaa aaaagagttg gaatcctttg gtttattatt acaaaagcag atgggattta
     2401 acaaacagtt agggaaaaat aatcctaata gactatatct agcagaatta aaagtctcag
     2461 aaaatgatat ctacttactc gaaaaatttg atagagagaa tagggaaaac gttgataaat
     2521 cagagggtat gaaaatcata cccaccctcg acgaaaaatc agacgctgaa tcccttgggg
     2581 ctcaagaggg tatgaaaatc ataccgtgcc aaaacgttga taaatcagag ggtatgaaaa
     2641 tcataccaga acttaataat aatatattag acactaatag acacaatata gacactgaaa
     2701 aagaccgcct acaagatcaa ttgttgttag acaattttga gacaattatg acaaacgaca
     2761 gcattgctac gtttgtccct gaacgagtat taaatttgat aaaaacattt tcttcaagtt
     2821 acagtgaagc tcaaaaaacc gtccagacta ttcataatgc aaagaaaaaa gctgaaatag
     2881 aaagtgatat ttcgatagtt tttgaagaac tcgatagtta ttatgtcaat gcagaacaag
     2941 aattatacac gacactgtta aaagcctatc aaaaattaaa aaccgaaaaa gtcgaaaata
     3001 tccagaacct gatttttgtc tatgtaaaaa attggtttat cgaaaaacca atagctgcta
     3061 aagtatcaag tgaaaaacgt ttgaattatg caagctcccc aagcactatt acgaaagact
     3121 ggttagagtg aaactttact gacaaagtga ttgttgcttc atggagtaac attaaaatta
     3181 aaaatttttt attattctaa ttatattacg aatattacaa aataatagct attttaatag
     3241 aatttataag ttaaaatatt atgataaaaa tcacaaacgt agccttttac gtcataagtg
     3301 ttaaaagaaa taattaattt caaatataga aaagggaacg tatgtttgca tactcctttt
     3361 ttttgagtta taataatacg cgaagagaga taatttcatt atttaaacaa gctaaaaatc
     3421 ttgatattac ttcttttcag gcaatacgaa tattattcgc ttacattttt tgtataaatt
     3481 taattgtctg ctatgcttgt gatgaattga ttttcgtttt gcacttttgg atgataaatt
     3541 tggacaattg aaaatacaaa aaaaagaaag gcggtatgaa ctcggaaggc tggtaacctt
     3601 cgtcagcgtt catacacggc attaagattt ggcccttaat gcgttaacct ttcagtgtta
     3661 cggtattacc cgcaatggat ttagtataac agatattcaa aagtgatacc actttttttg
     3721 gctgtgttaa ggttaaaaat acgtgatatt acgatgaatc tatgcctaag cataatgtta
     3781 taaaaatgtc cgtgtggctc tttgccgtat gacgaacgct gaaattagag ggaacgcatg
     3841 tttggatgcg ttccctcttt tttgtttgtc atgaacgaaa atcaattctc tatttttgaa
     3901 aaataacaag aaaaacttgt taaaaatatt taaagaaatt gggtggttta ttttatgtca
     3961 tatacaggaa ttttgagtct caaagatatt tgtcactacg gtaaacggtg taccgctaca
     4021 gagaaaatca cgaagaaact atcaactggc caaagtaaaa ccgttgtgca gtgtaagaaa
     4081 tatataatcc agaaagacaa agtctcagag gaaatgattt attacattgg gaaacaaaag
     4141 caaataattt taaaagatcc tatcccctta aaagagctat atccaacaat caaacatgtt
     4201 tatgaccaaa acggtgtact gattggtcga agaaaaaatg gagtcttgcg ttgtaccgca
     4261 aaagggatgg ggcgtttgat cggctgaatt gatcattctc ataacttgta ttttatcgtt
     4321 tttcgcttgc ttgaaaatat tgtcgagact tctaaaggaa cttagagtca ctagaaaaat
     4381 aaggagtaga cttgatctat caacaattcc cttcggggag tgacggggta ggttttaacg
     4441 attggtaacg taaaaaattg gtcaatcgag taaatgcgct tgtttggata gtggtgaaat
     4501 gaatttgtgt aaataataag cgcagagtaa acaagaggga atttagaagt tagacaagtt
     4561 agaatagcgt ctagattaat catgagcagc tactaaggga tctagacttt gacgcaaact
     4621 catggaataa ggagtaggtc ttctcgtaat ggggaggacg cacaagaaat tgtgtcgtgt
     4681 gctgcagtac atgtagatac ttgcgaggac gttgattatt ggatcaatcg agcatttacc
     4741 gctgtctact agggtttctc gtttcggacg ccataaacga gtcgaaattt tttatgtatt
     4801 tttaagtaaa cgaaaaacga taaaatatga acaagtcctg aataaagtga aaaaattaat
     4861 ataaagcata gagaaaatga aatttacgta atggaggaat gacaaatgaa aaattatgat
     4921 ccgaatatac gattgggcac acatactatt aaggtttctt tccaacgttg ggattacaaa
     4981 ggatttgtga cgtttagaag gggcggaaat tgtaaaggat tagatgtttt agcattagac
     5041 gaggatgatt tatacgatca aacattgact gataatccaa ttggattcgg cttgctacct
     5101 gaagatgatg aaggtaatga atggtttaaa atgacgctta tgaatgataa tggagacgaa
     5161 ttatctgttg aagatacttg gtcatatttg agtgattata ttgtcagctt tgaaatcatt
     5221 gaatttgtag cggataaaga agaataaaaa ctggaggaat aaattttaca gataaaggct
     5281 tagaaggagt cacgacgatt aataaaaagg tagtattacc ttaggcattg agtaagga
//
LOCUS       DACGLW010000129         5045 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.137, whole genome shotgun sequence.
ACCESSION   DACGLW010000129 DACGLW010000000
VERSION     DACGLW010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5045
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.137"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            complement(200..1318)
                     /locus_tag="IXT53_002765"
     CDS             complement(200..1318)
                     /locus_tag="IXT53_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906528.1"
                     /translation="MGNNSIYTPEMQNKIKRLVGLGWMTRGKLDSFSTIKYLLMDIYR
                     QNKQTGIHFLGYDKPEDAPFFVPSSAYRASTSGSMYNLDLFEEGAKMIEYDFNEAYSR
                     IMRNYPLPSNTYLKAKYNTDQAILRAINRPKVRPYENLRTYMFFLVNFEGTAKEGTYA
                     NPKSQSMFADYGENPKTANEPIWLSEIELTLILDYYDIKEMEVLDSHTFLTKKGLLKE
                     YFERIEPLKTDPDTLKLYKNLRTKVFGAIGQKSLREGETSTFDKYSMYNRAYSSMVAG
                     VFRDRMVRYEQKYVHSAYGLLKIRTDGLYFRSEVPEFEELYKLGVVKKKVHTISKEEV
                     EKALEFKNKGFENKGSFGKSKDEVKGVVIDDDFEFDFD"
     gene            complement(1347..2141)
                     /locus_tag="IXT53_002766"
     CDS             complement(1347..2141)
                     /locus_tag="IXT53_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906529.1"
                     /translation="MVAKKNTLKEASEKYQDMKRRGLIPKDKKGIWREFVKEVSSDRS
                     GGDFVGRYTAKGYSRRLRITVEYGTNESATELKQRYLDTRREASKKYDDFKSRGLIPK
                     KKKGIWREFFKEEDSNRNKGDFIGRYTAKGYTKNLGIKVEYGTGESSWELRNRYLNTL
                     LEKYKAELLELFDVNDFIEMAEKYGHEEGFMINDIMVIDNFTDLLALAKKARDLRETQ
                     MYLFRNKNGRNRRTESSEIEDYYDAFTHDLGAFVDEISGVMNKYRV"
     gene            complement(2151..3026)
                     /locus_tag="IXT53_002767"
     CDS             complement(2151..3026)
                     /locus_tag="IXT53_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906530.1"
                     /translation="MTTNKRWSELVQNTVKKVMPDGYHFNYIQCSQTTESIYFSILNE
                     NRLYYFRVSSHHRKKREDNVTTFLSGKYNGFKELSEAIGEYLKSNRFVRISDKHILIL
                     LLYLNNSFKLFWRDDTFYYIENGMDIYSGINGILKHYIKALLRYGVLYQEDLSNQIKI
                     SNSGKSLLAKYKKLLSPIYKGIDMEAVNSETLFNLVVDEKFANEFFKSKKIERLNNLF
                     PENAIKAYVDNNKSRLYNWFIPKRLRGKIKIGDQVKVINLNHTKIVTVTELYIAEKED
                     ITKMKPVIKILDNKE"
     gene            complement(3243..3500)
                     /locus_tag="IXT53_002768"
     CDS             complement(3243..3500)
                     /locus_tag="IXT53_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906531.1"
                     /translation="MINEDFRSENKMIDISLIEKLLFSNKYTAKELATRWQISESAIK
                     AYRQNKGSVGWRDWKGISIGKAEDIMKREGFQADNDSKKVE"
     gene            complement(3503..3727)
                     /locus_tag="IXT53_002769"
     CDS             complement(3503..3727)
                     /locus_tag="IXT53_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906532.1"
                     /translation="MNQRRRKRLEEISERLSELKDELEVIQEEEYKENIPKNLQGSER
                     YEKAEESVDQLEEAVDGLSDILELIEDIAS"
     gene            complement(3900..4943)
                     /locus_tag="IXT53_002770"
     CDS             complement(3900..4943)
                     /locus_tag="IXT53_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyhydrolase"
                     /protein_id="HAQ4906533.1"
                     /translation="MKKFLVGILFTMGLVLLSPSVSAESSISMYRMYNPNSGEHLYTR
                     SVGERDNLKRYGWHYEGVAWQAPTSGTPVYRLYNRYNGEHFYTVDAGERDKISHTGWT
                     YEGVAFYSYNGANRVPVTRLYNKYVNWHHYTLDQNEKNVISKQGWKIEGTGWYAMPNS
                     VPNPTPAPSTEKVLNVPVVWQGNTMLCEGASLLEGLHYKGVATNQSLTSFVNYMPKAN
                     DNNPYHGYSGEWRHNVNGTYQGMMADPVVKWAKNVGGNAVNITGSGTNGIKNEIRNGN
                     PVVTWITYNYATPEFKQMPWGRAVWNGHVVLVDGFRNGAYHVVDPVFGAKWINASTYE
                     RAFNVTGMAVAVR"
BASE COUNT         1377 a         1078 c          667 g         1923 t
ORIGIN      
        1 aaaaactgat ccccttttaa tctttctgta tcatccatca gaaattcaac taaatcttta
       61 tccatgcgtg caactttttc gtcttttagt gccattgatt tagcatagtc agttgaatct
      121 tttagaataa taacacctac atttccttcc acaattttct tcttcatatt tacctcctac
      181 catcttttcc aaagagtatt tagtcaaaat caaactcgaa atcatcgtct atcacaacac
      241 ccttaacttc atctttagat ttcccaaagg agcccttatt ttcaaatcct ttgtttttaa
      301 attctaaagc tttttcgact tcttctttgg atattgtatg aacttttttc ttaactactc
      361 caagtttata cagctcttca aactctggta cttcggatct gaaatacaat ccatcagtac
      421 gaatttttaa tagcccatat gcgctatgaa cgtatttctg ttcatatcta accatacgat
      481 ctctaaatac gcctgctacc atggaggaat acgctcgatt atacatactg tatttatcga
      541 aggtgcttgt ttctccttct cttaatgatt tttgtccgat agcaccaaat actttcgttc
      601 taagattttt ataaagcttc aacgtatcag gatcagtttt taatggctcg attctttcaa
      661 aatattcttt gagtaatcct tttttcgtca aaaatgtgtg gctatctagg acttccatct
      721 ctttaatatc ataataatca aggataagtg ttagctcaat ctccgataac caaataggct
      781 cattagctgt tttgggattt tcgccataat ccgcaaacat cgactgtgat tttggattag
      841 catatgttcc ttctttggct gttccttcaa aattaaccaa aaagaacata tacgttctta
      901 aattttcata cggtcttact tttggtctat ttatcgctct caaaatagct tgatctgtat
      961 tatattttgc tttcaaataa gtgtttgacg gcaaaggata atttctcatg atcctcgaat
     1021 aggcttcgtt gaaatcatat tcgatcatct ttgctccttc ctcaaacaaa tccaagttat
     1081 acatgctacc ggaggtactg gctctatagg ctgaacttgg aacaaagaaa ggtgcatctt
     1141 ccggcttatc atagcccaaa aaatgtattc ctgtttgctt gttctgacgg taaatgtcca
     1201 tcaaaagata tttgatcgta ctaaaagaat ctaattttcc tcttgtcatc caccctaatc
     1261 cgacaagtct tttaatttta ttctgcattt caggtgtgta tatgctattg tttcccattt
     1321 atctgctcat ctccggcttt tttattctag accctatatt tgttcattac accacttatc
     1381 tcgtctacaa aggctcccag atcgtgtgta aaggcatcat agtaatcttc aatctcactc
     1441 gattcagttc tccggtttct tccgttttta tttctgaata gatacatctg cgtttctctt
     1501 aaatcccttg cttttttcgc aagtgctaat aaatccgtaa aattatcaat aaccataatg
     1561 tcattgatca taaaaccttc ttcatgacca tacttttcag ccatctcgat aaaatcatta
     1621 acatcgaaca actccaataa ttctgcttta tatttctcta acaatgtatt taaatagcga
     1681 tttctcaatt cccatgaact ttcaccagtt ccgtattcaa ccttgatccc taaattctta
     1741 gtgtagcctt ttgctgtgta tcgtccaatg aagtctccct tattccgatt gctatcttct
     1801 tctttgaaaa actcacgcca aatccccttt ttcttcttag gaattagacc tctgctcttg
     1861 aaatcatcat atttttttga agcttctctt cgtgtatcta agtacctttg cttcagctct
     1921 gtagctgatt cattcgttcc atattcaaca gtgatcctta agcgcctgct ataacctttt
     1981 gctgtatatc gtccaacaaa gtcaccgcca cttcgatcac tactgacttc cttaacaaat
     2041 tctctccata tacctttttt gtcctttggt attaatcctc gtcttttcat atcttgatat
     2101 ttttcagaag cttcttttag agtattcttt tttgctacca tattttccac tcactctttg
     2161 ttatctagta ttttaataac tggtttcatt tttgtaatat cttctttttc agctatatac
     2221 aactccgtta cggtaacgat cttagtatga tttagattaa ttactttcac ttgatcgcca
     2281 attttgattt ttcctcgcaa ccgtttggga ataaaccagt tgtataatcg tgatttatta
     2341 ttatctacat aagcttttat tgcattttca ggaaacaggt tatttagtct ttcaattttt
     2401 ttacttttaa aaaattcatt cgcaaacttt tcatcaacaa ctaaattaaa taaagtttca
     2461 gaatttacag cttccatatc tattcctttg tagataggag aaagcaattt tttatatttt
     2521 gccagtaatg actttccaga attactaatc ttaatttggt tacttaaatc ttcttgataa
     2581 agaaccccat atcgaagtaa cgccttaata taatgtttta atatgccgtt gataccagaa
     2641 taaatatcca tgccgttctc aatatagtag aaagtgtcgt ctctccaaaa caatttgaag
     2701 ctattattca aataaagtaa caagatcaat atatgtttgt cagaaattct gacaaatcta
     2761 ttacttttta gatactcacc aatggcttca cttaactctt tgaagccatt gtattttccc
     2821 gataaaaatg tcgtaacatt atcttctctc tttttcctat ggtgtgaact taccctgaaa
     2881 taatagagtc tattctcatt taatattgaa aaataaatac tttcagttgt ttgtgagcat
     2941 tgtatatagt tgaaatgata tccatctggc atgacttttt taactgtatt ttgtactagc
     3001 tctgaccacc ttttgttagt agtcatatat ttttttccat taaaccaaac ataaatatct
     3061 tttttaaaaa ttttgttccc cccctattgc gttttctttg acattaaagc cctttgtttt
     3121 catcacattc gtatagccct agccactcat aacctgatgc ttttgcgaag tttatttaaa
     3181 agtgttttcc tttgtaatct acctttcccc tcgtcctggt acttttcttg gcgactatta
     3241 aactattcaa cttttttaga atcattgtca gcttgaaatc cttcacgttt cattatgtct
     3301 tctgcttttc caatgctgat tcctttccaa tcacgccatc ctacgcttcc cttattttga
     3361 cgataggctt taatagctga ttcggatatt tgccatcttg tagccaactc tttggctgtg
     3421 tacttatttg aaaaaagtag cttctctatc agtgaaatat caatcatttt attctcactc
     3481 ctgaaatctt cattaatcat ctctagctag ctatatcttc aatcaattct aatatgtcgg
     3541 ataaaccatc aacagcttct tcaagttgat caacggattc ttctgctttt tcatatcttt
     3601 ctgatccctg tagattttta ggtatatttt ctttgtactc ttcttcttga attacttcta
     3661 actcatcctt tagctcggaa agtcgctcgc ttatttcttc taaccttttt cttcttcttt
     3721 ggttcatcaa aatcacctct tcgcataatc tctacgattt taatatacca cttttgtgta
     3781 ctacattcaa tattttttgt tatagattcg tattacaaaa atttcctcta aaaaaataag
     3841 agcctattta ccatttcagt aattaagctc ttatttcatt tagttgataa ttctatcaat
     3901 taacgtacag caaccgccat tccagtcaca ttaaatgctc tctcatacgt gcttgcattg
     3961 atccattttg cgccaaatac agggtcaaca acatggtatg caccatttct gaagccatcc
     4021 acgagaacta catgtccatt ccatactgca cgaccccaag gcatttgttt gaactctggc
     4081 gtagcatagt tataagtaat ccaagttaca accggattac cattgcgaat ttcattcttg
     4141 attccattcg ttcctgaacc agtgatattg acggcattac cgccaacgtt ttttgcccat
     4201 ttaactactg ggtcagccat catgccttga tacgttccat taacgttatg acgccactct
     4261 cctgagtaac catgataagg attattgtca ttagcttttg gcatatagtt tacaaaactg
     4321 gttaagcttt gatttgtggc tacacctttg tagtgtaaac cttctaataa agaagctcct
     4381 tcgcataaca ttgtatttcc ttgccataca acaggaacgt tcaacacttt ttcagtgctt
     4441 ggagcaggtg taggatttgg tacgctgttt ggcattgcat accagcctgt accttcaatc
     4501 ttccaacctt gtttactaat tacgttcttt tcattttgat ctaacgtgta gtgatgccaa
     4561 ttcacgtatt tgttatataa acgtgttaca ggaactctat ttgctccgtt gtaggaatag
     4621 aacgcaacac cttcgtacgt ccatcctgtg tgtgaaatct tatctctttc tccggcatca
     4681 actgtgtaaa aatgttcacc gttataccga ttatacaaac gataaacagg agtaccactt
     4741 gttggagctt gccatgcgac accttcgtaa tgccatccat aacgtttcag attgtctctt
     4801 tctccaacgc ttctagtgta tagatgctct ccgctattgg gattatacat acgatacatg
     4861 gaaatagagc tttctgctga aactgaaggt gataataata ctagccccat tgtgaataga
     4921 attcctacta aaaatttttt catttgattt tacccctttt cattttttat tgtcattatt
     4981 gcatgaaaat tattcgtaag attgccaatt acataaacga ttgttttaag atcataatcg
     5041 ttgtt
//
LOCUS       DACGLW010000130         4931 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.138, whole genome shotgun sequence.
ACCESSION   DACGLW010000130 DACGLW010000000
VERSION     DACGLW010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4931
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.138"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            85..972
                     /locus_tag="IXT53_002771"
     CDS             85..972
                     /locus_tag="IXT53_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HAQ4906534.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1112..2038
                     /gene="cysK"
                     /locus_tag="IXT53_002772"
     CDS             1112..2038
                     /gene="cysK"
                     /locus_tag="IXT53_002772"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HAQ4906535.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2274..2720
                     /locus_tag="IXT53_002773"
     CDS             2274..2720
                     /locus_tag="IXT53_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HAQ4906536.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2993..4345
                     /locus_tag="IXT53_002774"
     CDS             2993..4345
                     /locus_tag="IXT53_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase"
                     /protein_id="HAQ4906537.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4516..4803
                     /locus_tag="IXT53_002775"
     CDS             4516..4803
                     /locus_tag="IXT53_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906538.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT         1695 a          813 c         1030 g         1393 t
ORIGIN      
        1 aattaaaagt ataatttaat taattaagtt tgttttaaga agagaacttt ttattaagtt
       61 tttttatttt gaggaggaat ttgaatgcaa gagctgaaga cccgcagtgc ccggcaccat
      121 agacctaaaa gaaaaatggt gatcaataaa aaagcgattg ctcctttaat tggaacgagt
      181 cttttagttg ggccgatgat ggttacagcg gtaccgcaac atgttcatgc agatgaacaa
      241 ggatctcaaa cagtcgctga tcctcaagcg tttatcgatc aaatcggctg gtctgcgtca
      301 gaagttgctg caagtaatga cttgtatgct tcagtcatga tcgctcaagc gttgttggaa
      361 agtgcatatg gcacttccgg attagctagt gcgccaaatt ataatctatt cggtgtgaaa
      421 ggaagctata acggacaggt cgtttatatg gcaaccaaag aatatgtaaa cggacaatgg
      481 gtaacgatga atgagccatt ccgaaaatat tcttcttatt gggaatcctt ccaagaccat
      541 gcgaatgttc ttcgttcaac cagtttttcc acgggcgatt atcattatgc tggcgtgtgg
      601 aaaagcaata caacaagttt ttatgatgca acagcgtact tgacaggtcg ttatgctacc
      661 gatccaaatt atggggccaa attgaactca ttgattaata cttataattt aacccgtttt
      721 gatacgccat caacaggttc ggctacaaca accacaagta caacaacaac gactactacg
      781 acgacttcaa cgtcatcatc ttcaacaaca gatgtaaata caaataatgg tcagacatat
      841 acagtcgtgt caggtgatac gctatgggat attgcacaaa aatttggcat gtctgttgat
      901 gatctaatgg cgaaaaatgg catgtctatg tccagttatt ctttattagt tggtcaagaa
      961 ctaaacgttt gatttagata aaaaggcaaa gaaattacca tatatggtat atctttgtct
     1021 tttttttgct agtcaaagac gaatcactcg ccataaatga acagattgta ttatgataag
     1081 taagaagagc aaagacaagg aggaattgaa gatgacagct atctataact cagtcaccga
     1141 attgattggc aaaacgccaa tcgtcaaatt aaataaaatc gtaccagaag attcagcaga
     1201 tgtttttgta aagcttgagt tttttaatcc aggaggaagt gtaaaagatc gtattgcttt
     1261 aagtatgata gaaaaagcag aacacgatgg tctactgaaa ccaggtgata ctatcattga
     1321 acctacttct ggaaatacag gaatcggatt gtcgatggta ggagttgcaa aaggatataa
     1381 agtaatcatc gtgatgcctg agacgatgag tatcgaaaga cgtttattga tgaaagggta
     1441 cggcgcagag ttgattttga ctcctggtgc ggatggtatt tctggatcaa tcagagaagc
     1501 agaacgtttg gcaaaagaaa acggctactt tttaccatta caatttgaaa atgaagcaaa
     1561 tcctcttgtg catgagaaga cgactggtcc tgagatacat caagcatttg gagtaaatgg
     1621 cttggacgcg tttgttgcag gtatcggtac aggaggaacg atcactggcg ctggccgaga
     1681 attaaaacgt gtgtatccga aaatcgaatt aatcggggta gaaccagcag aatctgctat
     1741 tttagaaggg aaagaagcgg gacctcacaa aatccaagga atcggtacag gttttgttcc
     1801 taaaacactg gatacttctg tgtatgataa agtcctttct attagtggag acgaagcgat
     1861 ggaaacagct agagaagttg ggcgaaaaga agggatttta gtaggcatct catctggcgc
     1921 tgcaattgct gccgcgctaa aagtcgcaaa agaattagga aaaggcaaga aggtattagc
     1981 tgtggttccc gacaatggtg aacgttattt atctacagcc ctttaccaag agatataagg
     2041 cacttggttt ttctccagat aggtttatgt aaaaaacttt taaagatgaa aagaacacac
     2101 aaaaatttat gatgagaaaa attatttgaa tagagacgta gaatagtgaa cgcgactgtt
     2161 ttacgtcttt tttcattaaa atacaataaa attgaatttt cttttcatgt taagtggatt
     2221 ttaaaatgta aaaatagtat gataagacga gaataacaac gtggggtgtt tacatggaaa
     2281 ataagttagt cgaagaggtt atcgaaaagt taaaaaaaga gaacattcga atcacacctc
     2341 aacggtacgc ggttttagaa tatttgattg aacatcattc ccatccaacg gctgatgaga
     2401 tttataaagc acttgaacat cggtttccaa atatgagtgt tgcgactgtt tacaataacc
     2461 ttcgcttatt tacttcgatt ggatttgttc aagaaatgaa gtacggcgat gcttctagcc
     2521 gatttgattt cagttcaaag cgtcattatc atgtaatttg tgaaaactgc ggtaagattg
     2581 ttgattttca ttacccaggg cttgaagata tagaaatggc tgcaagcaga ctgactgatt
     2641 ttgagattaa tgaacatcgg ttggaattgt atggactctg tccggattgt aaagagtcag
     2701 caaaaaaggc gcaaatgtaa aaacagtttc tacaagtgtt atacctaaaa aaagctagct
     2761 gtgtaaaaaa aacgctagct tttttatttt tttgtctttt attcacaatc ttagatataa
     2821 aagtgaatat tattaaaata agaatcttgt agatattggg ttcttatgct tgaaaaggaa
     2881 tgaaactaca aacaaacctt tgtgaattca caagcattaa actgttgtat ccgttttatt
     2941 tctgttatag tagttttgtc gaaggacaaa atgttaatcg gagggaccat aaatgaaagt
     3001 agtagtaatt ggctgtactc atgcaggtac tgcagctgta aaaagtattt taaaaaatca
     3061 tccagaagca gaagtgacag tatatgaaag aaatgacaat atttctttct tatcttgcgg
     3121 tatcgcactt tatgtcggcg gagtagtaaa ggacccagca ggcttgttct actcaaatcc
     3181 tgaagaatta tcttcattag gagcaacagt taaaatggaa cacgatgtaa cagacgtcga
     3241 tacagaaaat aaaacagtaa cggcaaaaga tctgaaaaca ggagaaacag aaacggtttc
     3301 ttatgataaa ttagtgatga caactggttc atggcctatt atcccaccga ttagtggaat
     3361 cgatgcgaaa aatgtactcc tatgcaaaaa ctacaatcaa gcaaatgaaa ttattgcaca
     3421 agcgaaagaa gcaaaacgtg tagtaattgt tggtggtgga tatatcggta ttgaattagt
     3481 agaagctttt gtcgaatctg gaaaacaagt gacattgatt gatggattag atcgcatctt
     3541 gaataaatat ctagataaac cattcacaga tatcttagaa aaagaattga cagaccgcgg
     3601 tgttactctt gcgctaggcg aaaatgttac tgaatttatc acagatgaag aaggaaaagt
     3661 gaaacaagta gctacaccaa cagatacatt tgatacagat atggtcattt tatgtgtggg
     3721 cttccgtcca aatacaaaac tgatcgaagg aaaagtagat acactgccaa acggggcaat
     3781 caaagtgaat gaatacatgc agacaagtaa tccagacatt tttgcagctg gagactctgc
     3841 agtagtcaac tacaatccaa gtggcacgca aaactatatt cctttggcaa caaatgccgt
     3901 tcgtcaaggt ttgcttgtgg gaaataactt gacagatcat aaaatggcgt atcgtggtac
     3961 tcaaggaact tcaggacttt acctatttgg ctggacgatc ggttcgacag gtgtgaccaa
     4021 agaaagcgca actttgaacg atctagaagt ccaagcgact gtatttgaag ataattatcg
     4081 tccagagttc atgccaacta ctgaaaaagt aatgatggag ctagtctatg aaaaaggaac
     4141 gaatcgtatc gtaggtgctc agttcatgtc caaatacgac attacacaat cagcaaatac
     4201 catgtcttta gctgttcaaa ataaaatgac tgtagaagat ctcgcacttt cagatttctt
     4261 cttccaacca cattttgacc gcccatggaa ttacttgaac ctacttgcac aagcagcgct
     4321 aggcgaaatc gaaactgcac aataaagaaa aagggttgtg acacaatttt gtcacgaccc
     4381 tctgtctttt tttgtgaaaa gtacaattag ttgtctttct tcgaattttt tgtcacaata
     4441 agcagcaaag ataaagctgt tacataaaag aacaagcagc tttatttgat tttgaaaaga
     4501 aaggtgagaa agaagatgaa taaagaatat tttgtccaac aagatgcatt tttagaagat
     4561 tataaaagtg cttcgaagaa attaacgatc aatattgatg agttggcaga tcaagtactc
     4621 tctcagttga atgaaacgca agaggaatat tacacgatgc cagcaaatga cgccaaagat
     4681 aatgtttctc atcgttttcc tttcagtaga cgcatctact cagatgaaaa tgacggcaca
     4741 ttgaatacag agtacacata tgaaggtcag ccatatgtgc tagaaactga tgatgatctt
     4801 taaagacagt ttttgaaaag gccgctcatc cttaagtgat gacaaaaaat catcacaatt
     4861 accgatgact gtctctttac tatcttttaa tcagaaaaag gggttatgac aagctttgtc
     4921 aacccctttt t
//
LOCUS       DACGLW010000131         4809 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.139, whole genome shotgun sequence.
ACCESSION   DACGLW010000131 DACGLW010000000
VERSION     DACGLW010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.139"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            209..307
                     /locus_tag="IXT53_002776"
                     /pseudo
     CDS             209..307
                     /locus_tag="IXT53_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(530..760)
                     /locus_tag="IXT53_002777"
     CDS             complement(530..760)
                     /locus_tag="IXT53_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HAQ4906539.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(990..1808)
                     /locus_tag="IXT53_002778"
     CDS             complement(990..1808)
                     /locus_tag="IXT53_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HAQ4906540.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            1969..2658
                     /locus_tag="IXT53_002779"
     CDS             1969..2658
                     /locus_tag="IXT53_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HAQ4906541.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            2672..4174
                     /locus_tag="IXT53_002780"
     CDS             2672..4174
                     /locus_tag="IXT53_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010759843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ4906542.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            4187..4663
                     /locus_tag="IXT53_002781"
     CDS             4187..4663
                     /locus_tag="IXT53_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HAQ4906543.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
BASE COUNT         1608 a          701 c          858 g         1642 t
ORIGIN      
        1 aggggcagcg agtgtcgacc ccttctaggg ggattaaaaa tacacccgtc ctcacgggtg
       61 tatgggttac tgatagactt gattttaact ggtgtcagaa acttatgtcc agaaagggtg
      121 atgaaatttt ggcaatttac ggctattcaa gaattagcac aagcaaccaa gattacaaaa
      181 cacaaattca aacattagaa aatgctggcg cagaaataat cttcctagaa aatcactgga
      241 ttatcgaaca cctttggaag tatttttgag ttacgtaagt attgatgatc tgtctaactt
      301 aatttgacaa ttaagagtat atttacttca gatggtaagg caacaaaata aaatacaata
      361 ggcagatgaa ctaataaata aaatctgtat taaatacaga taaattaata taaaaatcaa
      421 aaggatcata aacttttaac aagtgatgaa atgggtggaa attgattctt atagacagtt
      481 tgtctcataa cattgaagta ttttgcaatt ttagcattga aacacttttt taatcttcaa
      541 cgttgttctt ataaacaaga tgattgggaa tcttttgaat taacactgta tagcttagaa
      601 caccctttat agaaaacatt cgcttagcaa tcttaataat tccttgtgcc cgatgtcgtt
      661 tagattcagt aggttcctgc tcatcgatca tggaaaaaat ttgaaggatc agatccttaa
      721 taaatttatt taagagagga tgatccattg cttcatccat actcgataaa ctagtgataa
      781 tgagctacac gtttttcttt caagttgatc aatagcttcc acgataaaaa tatctttaga
      841 taaaatcaag aaccgtagga actacaggga tatttggtca ataagagcca ccgctattag
      901 taaaaaataa gctatcaagt atgctctcat cccaagaatt gcctacttca atcgttaaaa
      961 accgttaggt ttagctaagt gggggtagtt cactcaatac ttttatcaat aatagagcgc
     1021 aaaacttttt ctcttaattc aactgaatca atattattgt tttgttcata accatgaact
     1081 aatagatcac aaatataaag ttgtgatatt tttccagcta aagatccacc attaagaaat
     1141 tcttcaatgg ctgtttgtaa aactaagtct gcagactttc ctataggtga atgaatatag
     1201 ttagttatag caataatttt tgcgccattt tttttagcta ttttcaatga atcataagta
     1261 tctttcgttt tcccagataa agaaaaaatg attactaaat cattaactgt taataaagaa
     1321 gcgacttggg cttgaaaatg agggtctagt acagcttttg cttgaactcc tacgcgaaga
     1381 aacatatttt ctaaatcaag actagtattt ccactagatc ccactccaaa tatatatatg
     1441 ttttttgatt gagtgatcat tttaatggct tcattcaatt tttcttcgtc taaaagccta
     1501 atagtggaat agagagcttc tgttaaacta ttagcaattt catcatagta ctttacacta
     1561 gtcggatgtt cttttttttg actaaagtct tcctttgcaa tttcaatttt taaatcagaa
     1621 aaaccagaaa aaccaatttt ttgacaaaag cgaataatag tagcatcgcc aacttttgtg
     1681 tttttcttaa tatcactcat cgtactataa ataatagttt tcccagaatt taaaataaaa
     1741 tcagcgactt ttttttcaga cttcgttaaa aatggataat tttctttaat taaatagata
     1801 taactcatac aaactcctct acaatttcaa cttttctcca ttatactcca aaataataaa
     1861 aaaaactcct aaatactcca tttttatctt tacttttatg aaagcggatg ctatattgtt
     1921 tttggagtta ttatccaaaa ataataaaaa attggagcgt ttaggataat ggataaaaag
     1981 aggtttttag atcttatttc aaaaggtttg attgtttcat gtcaagcatt acctggtgaa
     2041 ccattttaca aagaggaaga aagtttaatg ccttattttg ctttggctgc tcaaagggct
     2101 ggagcagttg gtatacgtgc aaattctgtt cgagatatta ttgaaattag aaagtgtgta
     2161 tcattaccaa tgattggaat tattaaaaga gattatccac cagaatctcc attcattact
     2221 gccactatga gagaagtaga tgaattagta gaaaccggtg ttgaggtaat cgctcttgat
     2281 tgcactctta gaaatcgaca tgatggacta tctataaatt cttttattac taaaataaaa
     2341 gaaaaatatc ctcatcagtt atttatggca gatatttcta catttgaaga aggcaaaaat
     2401 gcatataaag caggaataga ttttgtagga actactttaa gtggatatac tgaggaaagt
     2461 aaaaaacaag atgggccaga tatagatttg attaaaaaat tggtagaaga agggatttgt
     2521 gtaattgctg agggaaggat tcatactcca gaacaagcaa aagagataaa aaaaatagga
     2581 gttgcaggta ttgttgtggg tggtgctatt actcgaccac aggaaattgc atcaagattt
     2641 gttaaagcat taagttaggg ggagaaataa aatgtttaaa aaattttcac aattaggacg
     2701 cgctttcatg ttaccaattg ctatattacc agtagccgga ttactattgg gactgggagg
     2761 agcgttaaca aatgagagtg ctataaatgc ttatccattt ttagatcaac cgtggttgca
     2821 tacaatttta agtattatga gttatgctgg aaatgcagtt tttgcaaacc tagcactaat
     2881 ttttgcaatt ggtattgctg taggacttgc aaatggagat aaaggaacag ctggtttagc
     2941 tggtggagta gcttatttag tatatactgc aactattagt gggtttcttg aacttttttc
     3001 agcaaaagat gcaacactag atacaggtgt agtgggatca attgttgttg gtggtactgt
     3061 tgcattttta cacaatcgtt atagaaaaat tgaattacca caattcttag gtttttttgg
     3121 aggatcaaga tttattccga ttgtttctag tttagctgca atcattattg gttctttctt
     3181 ttatttaatt tggccaccaa ttcaaaatgg tttagtagtt gctggtgaac aaattgccca
     3241 aatgggtagt ttaggaactt ttttatatgg ttttttattg cgtttaacag gtgctgttgg
     3301 actacaccac accatttacc ctttgttctg gtatacatct ttaggtggta ccgaaactgt
     3361 agctggatca acaattgctg gagcccaaaa tattttcttt gcccaactag ctgatccaaa
     3421 ccacactggt ctgtttactt atggtactag attttttgca ggaagatttg caacaatgat
     3481 gtttgggtta ccagcagcat gttatgcaat gtatcgtgca attcctaaga aaaatcgtaa
     3541 aaagaatggt gggctttatt ttagcggggc ccttacatca tttttaactg gaattactga
     3601 accagttgaa tatatgtttt tatttgttgc tccttggctt tatgtgattc atgcattctt
     3661 agatggatta tctttttatt ttgctgatat cttaaatatt cgtgtaggga attccttttc
     3721 aggtggtttg atcgactttt tattattcgg tgtacttcaa gggaatgaca aaactaattg
     3781 gttaaaatta attccttttg gtattgcatg ggcatttata tatttctttg tttttacatt
     3841 ctttatcaaa aaatttaagg tagctattcc tggaatggaa gaagaatcag aagttcattc
     3901 tgaaaaagta ataaacaaaa atacatcatt acatgaagag tctcttatca ttattgaagc
     3961 tcttggtgga aaagaaaata ttgagtctgt tgaagcttgt gcaactcgtt tgcgtgttgc
     4021 tgttacgaat ggggagatta ttcaaaaaca aacaataaaa gacactggtg caactgctgt
     4081 cttcgaagtg aagggtggca tccaagctgt ttttggaggg aaagctgact tattaagtca
     4141 agaaatcaat caaatattag gaaaagataa ttaggaggtt tttaatatgt ttgaaatttt
     4201 caaaaagaaa aaacaagaaa ctttttatgc accttgttcc ggaaaaatca tttccatcac
     4261 agaagtaaat gatcctgtct tttcacaaaa attactaggt gatggttttg ccgtcatacc
     4321 tgatggttca aaaatttatg ctccattggc tggtcgagta acaaatatat ttcctactaa
     4381 acatgctatt aatataaaaa gcaactcagg cattgattat ttgattcata ttggaattga
     4441 tacagttgaa ttacaaggag agcctttttc gatttgtgtt caagaaaatg atgaggttac
     4501 tcctgaaacc tcgttggttg atgtggattt tgacaaaata ataaaatctg gtaaagatac
     4561 ttctgttata gttgtttttc cggagaaaaa tcagttcaat gatttaataa tagataatgg
     4621 tcgaattgct catgggaatt catgtggaaa aatcactttt taaaaaagtg tatataaaaa
     4681 gctataaaat aagctaaata ggatatttgt caaatgaagt tggtgaacag aaagaactgt
     4741 tttctttctg ttcatttttt tattttatta gtaatgattc cccatgccaa atcatgatcc
     4801 gtaatttaa
//
LOCUS       DACGLW010000132         4733 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.140, whole genome shotgun sequence.
ACCESSION   DACGLW010000132 DACGLW010000000
VERSION     DACGLW010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.140"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            23..694
                     /locus_tag="IXT53_002782"
     CDS             23..694
                     /locus_tag="IXT53_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ4906544.1"
                     /translation="MNFLVVLKDTLIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            753..1193
                     /locus_tag="IXT53_002783"
     CDS             753..1193
                     /locus_tag="IXT53_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906545.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1205..1873
                     /locus_tag="IXT53_002784"
     CDS             1205..1873
                     /locus_tag="IXT53_002784"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HAQ4906546.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1937..2560
                     /locus_tag="IXT53_002785"
     CDS             1937..2560
                     /locus_tag="IXT53_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HAQ4906547.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2616..3002
                     /locus_tag="IXT53_002786"
     CDS             2616..3002
                     /locus_tag="IXT53_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HAQ4906548.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3215..4606
                     /locus_tag="IXT53_002787"
     CDS             3215..4606
                     /locus_tag="IXT53_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HAQ4906549.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
BASE COUNT         1575 a          782 c         1007 g         1369 t
ORIGIN      
        1 tgatgaaaaa agggggagaa aaatgaattt tttagttgtt ttaaaagata ctctgattga
       61 gcgatctaca tttctatatg ttcaattgca ggaaaataaa acattgctgc atttttcacc
      121 agagttccat ctagaggggc taacattagg agaaatctat caagcagaag ggctgtcggc
      181 tgttttgcac ttttttaagg tagaattgga attaccagta aaagattata tcgagctttc
      241 tcttgaatct tgggatcgag cagtggttga actgtttcca gaaggtttaa tgatcgatac
      301 aaacgatggg aagatccatc tgactgcagc agaactgcaa aaacaaatgc gttatcaaat
      361 agatgacgaa aattcttttt ctatttttag acgtcagcaa aagattctaa aaagcttttt
      421 aaaagtgatc agtaaaaaac ggaatctgat caaaacgaca cagctgttga aaaaatatcc
      481 aggtctgctt cacacgtcta ttcaatttcc tcaattgatt acgatgatcc atcgttttat
      541 acgtatgcaa cagctaccat ctaagaagct gtttcttcca ttgacggaca catttcgagt
      601 agtcaacaga gaagataaaa agaaagtgat tgtgattgac tttaccagaa atcggaatgt
      661 ccttcaccaa gttgcaatgg aaaaggccaa ttaaggcttt ttctttttgg atttttttgt
      721 cataataaat attagatgat ggaggagata atatgatgca aacatggtta gaaaggctaa
      781 tacgatttca gcagaactat cacaaactta tgcagggaag atatgcgaaa tttgatcagt
      841 tgaatcgcgg tctattgatc gctgcgcttg tctttagttt gctaagtcgt tggttgccct
      901 atagaatcgg acaattgttg ttcatcgtct ttttcggatg gatgatattt cgtttcctat
      961 caaaaaaaat ctaccccaga ctaaatgaga atcaacgtta tttgaaatat cttgatagag
     1021 tcaaagaaaa atatcatcag ataaaaaaca aaaagcaagt caaaatggat cgaaaaagtt
     1081 atactttttt tgagtgcccg aattgtcatc aaaagcagcg tgcacctaaa ggaaaaggac
     1141 gaatacgtgt gacatgcaaa acatgtggta taaaattcga aacgaatgtt taagggagaa
     1201 aagaatgaaa ttagtattat tacgtcatgg cgaaagcgaa gcaaattttg aaaattactg
     1261 gacaggatgg ctagatgtcg ccttaacaga aaaaggaaaa gaacaagcag cagttgccgg
     1321 tcaaaaaatg cttgaagccg gacttcaatt cgatgccgtt tatacctctg ttttgaaacg
     1381 tgcgattctt accggacaag tcgcactaga agaaatgggt cagttatggc ttcctataat
     1441 aaaaacatgg cgtctaaatg aacggcatta tggggatctt gtgggtaaaa acaaagataa
     1501 gatgaaagaa atatacggaa aagatcaagt aaaaacatgg cgcagaggat atacggaagt
     1561 tccgccgtta accgcagaaa atagatttga ccgcagatat gatcacttgg atccgcattt
     1621 gatcccatac ggagaaagtc tggaaatgac agtgaatcgg atcattccct tatggcaaga
     1681 tcatctagca ccaaaactaa aagatcggca agatttacta gttgtgggtc atggtaatag
     1741 catccgagca ctgacaaaat atttagaaga tgttcctgaa gatcaaatgg atacgatcga
     1801 tatcccaaat gcacaaccaa tccagtatac attagcagat gatttgacaa ttctggacaa
     1861 aaaaatactt taaagaacac ctttatctaa aagtgagaga tagaaaaaag aagcaaagga
     1921 atggaacaaa aggaacatga aaaattatat atgggacttt gatggtacat tgtttgatac
     1981 gtatcccgca atggtagatg gtgcatggca agcactaaaa gattttggta tcagtatgga
     2041 caaaaaagaa atctacttca aaatgaaaaa gtattctact agttatttga tcaatgaatc
     2101 aaacttgaat gccagagagt ttaatgaatt atttcaccga tatgaaaaag aaagtacgga
     2161 agtttcccgt ccatttccag aaacgaaaca agtactggaa atgctaaaag acaacggcgg
     2221 tcgtcatttc atcctgaccc accgtttgac agaatcaaca tggggcctgt taaaagaaca
     2281 tcgattggca catctgattg aggaagtagt agggatcgat caggactttc ctcgtaaacc
     2341 tgatccagca tctttaaatt atttgatcga tacttttcat ttagaaagaa cagacacgat
     2401 gatgatcggc gatcgtcgtt tagatatcga agcagggaaa aatgcaggag tcgctacttg
     2461 tctttacgac atcgatcatt ttctgggtga gatcccggca gattatgtgg taggaaatct
     2521 gaatgaaatc cttaccttgc agagcatcat taaaaagtag tatccccttt caaaggagat
     2581 atgttataat tttcataaag gagtgataaa attatatgga aatcagaaga caaaaagaag
     2641 ttgtagaagc atttcattat gatatgagat tgccgaacca agaagtagaa acagatttga
     2701 aagtaggctt ctcgccttta aaagaaacga tcgaaaacta tccgaaagaa catagcatcg
     2761 taggagcacg cttagagttc cgtttagtgt ttgatacata tgttttatct ggaagtgtga
     2821 gtcagatcaa tcatatcctc aatcggaaaa ttgaaggtca aaatgatgtc acacaagaag
     2881 aagtggacga gctagtagca ccgttattcg atatcgtacg ccgaatgtct tatgaggtga
     2941 cagagatcgc cttagatcag cctggggtaa aattgaattt tcaatcagaa aaacaagaat
     3001 agacagtaaa aacatacctg cgatagatta aagctatcac aggtatgttt ttttattact
     3061 aattgtaagc aaaaagaatg tcatttcgat aaaaatcgtc taaaatataa aagtcactga
     3121 aaaaaatttt ttcaggagga tggttgaaga atcattctcg ggaaccttta cttccttcga
     3181 tggacttgtc attaataata tgaggaggag aaaaatgaca acactcatca cagttttagt
     3241 ggttctagtt tttgcggcat tattatatgt gttctatcgt atgcaaaagc gccatacaaa
     3301 attttcaaca cgcgtcttta cagcattagg agttggtatc gtctttggcg gtctgcttca
     3361 attgatcttt ggtgtagacg acaaagtcgt tactcaatca atggaatgga tcgggattgt
     3421 aggtcaagga tatgtatcat tcttgcaaat gctagttatg cctttagtgt ttatttcgat
     3481 cattggtgca ttcacaaaaa tgaaagtttc tgaaaagcta gggaaaatta gtgcgactgt
     3541 attgacttct ttacttggaa caacagctat tgcagcattg atcggaattg gttcaacaat
     3601 gctgttcgga ctccaaggag ctaaattcac acaaggagct gcagaaacag cacgaatctc
     3661 tgaactggca acacgtcagg aaacagttga aaacttatcg attccacagc agattttatc
     3721 ctttatccct aaaaacgttt ttgctgattt agcaggtagt cgtgcaacaa gtacgattgg
     3781 agtcgttatt ttcgcggcat ttgtcggaat cgcttatcta ggcgtgaaaa gaaaagcgcc
     3841 aaaagaagca gcattctttt cagatttagt cgacagtctg tataaaatca ttatgcggat
     3901 cgtgacgctt gttttgcgtt tgacacctta tggagtattt gctttgatga caaacgtttt
     3961 ggcaacaagt gattttgcag ctatcgtcaa tttaggtaaa tttgtcgttg cttcttacgt
     4021 agcattgctt gctgttttga ttgtccactc actgattttg attggtgcaa aagtcaatcc
     4081 aatcaactat ttcaaaaaag cctttccggt attgagcttt gcgtttactt cacgttcaag
     4141 tgctggagct ttgccattga atatcgagac tcagacaaaa gctttaggag tagatgatgc
     4201 aacagctaat ttctcagcaa gttttggttt gtctattgga caaaacggct gtgctggcgt
     4261 ttatccagct atgttggcaa cgatcgtcgc accagcagct ggagtgaatg tctttagttt
     4321 ggaatttatt ttgatgcttg tggcaattgt aacaatcagt tcttttggtg tagctggagt
     4381 cggtgggggc gcaacatttg cttcactgat cgttttaggg gcaatgaact tgccagtagc
     4441 aatcgttggg ttagttatct ctgttgaacc attaatcgat atggcacgta cagcagtaaa
     4501 cgtcagcggt agcatggtag ctggtatcgt gacaagtgca cgcatcaaag atttagatcg
     4561 cgatgtcatc aatgatgatt ctaaagtgat cgatgtgaat gcatagtcaa aaagaggttg
     4621 tgatagaagt ggtcagcttt aagaaataag aagaagtttc ctaaaaatag tttctcaaat
     4681 ttttgtgaaa tttcggctta tttccgagaa gctacttctg aaacaccgtg aat
//
LOCUS       DACGLW010000133         4587 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.141, whole genome shotgun sequence.
ACCESSION   DACGLW010000133 DACGLW010000000
VERSION     DACGLW010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4587
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.141"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            complement(1..648)
                     /locus_tag="IXT53_002788"
                     /pseudo
     CDS             complement(1..648)
                     /locus_tag="IXT53_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
     gene            complement(970..1191)
                     /locus_tag="IXT53_002789"
     CDS             complement(970..1191)
                     /locus_tag="IXT53_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906550.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(1289..1534)
                     /locus_tag="IXT53_002790"
     CDS             complement(1289..1534)
                     /locus_tag="IXT53_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906551.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1774..2388
                     /locus_tag="IXT53_002791"
                     /pseudo
     CDS             1774..2388
                     /locus_tag="IXT53_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2758..3759)
                     /locus_tag="IXT53_002792"
     CDS             complement(2758..3759)
                     /locus_tag="IXT53_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HAQ4906552.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFDPIEFDNDERL
                     LLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSYLSDRVFDVLEKLSKSGILKEEYD
                     SSFDEYPSRYILISEDYKKFKRYFLKNEKKISVIMEKYKAPIQADPFSNLDESDLPF"
     gene            complement(3918..4184)
                     /locus_tag="IXT53_002793"
     CDS             complement(3918..4184)
                     /locus_tag="IXT53_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ4906553.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(4174..4530)
                     /locus_tag="IXT53_002794"
     CDS             complement(4174..4530)
                     /locus_tag="IXT53_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ4906554.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT         1425 a          843 c          639 g         1680 t
ORIGIN      
        1 agtagacacc gaaaagccga agagcgttcc atttcttcgg ttctttttat atattcctcg
       61 aatggtctcc atgcccttaa tcgtggaaga ggctgtacgg agactttgat aaaatttatt
      121 tcgtcgttta ataggtcgat ggtcttgttc tattaaattg ttaagatact tcacagttcg
      181 gtgctctgtc ttagtatata aacccacact ctgtaacttt ctaaaggcgg agccaagaga
      241 aggtgcttta tcggtcacaa ttgctttcgg ctcaccaaac tgtttatgga gtcgttttaa
      301 gaaagcatag gctgcttgcg tatcccgttt ctttcgtaac cagatatcta aggttaagcc
      361 gtccgcatca attgcacgat aaagataatg ccaacgtccc ttaattttga tataggtttc
      421 gtccattttc catgaataga aggattgtct atttttcttc ttccaaagat aatagaggac
      481 tttgctgtac tcttataccc aacgataaat cgtagtatga caaacattta ttccacgatc
      541 atataacaat tcctgaactt cacgatagct tagattgtaa cgcaggtagt aaccaacagc
      601 gacaataatg acgtcttttt tgaattgttt gcctttaaaa tgattcatta ctctgtcctc
      661 tctgtctttt ttctcaattt tacactaaaa tagatttttt ggaaaacttt gcaacagaac
      721 cgtttttact atatacttct tcccagaact tgtccgtctc cttcaaatta tctctacctc
      781 tcgagttcat tgggtggttt gcccatttat taaacatgga atcacctttc tcatactcat
      841 atatttatgt cctatttcaa ttatatttat atatgcttaa ataaatttgt atcctgaaat
      901 ttattgtaat atgaagctgt attttacata tagtaaccaa agagttaata aaataaaaag
      961 agcgaaccat tatagatcag ctcttttttc attgctgttt ttaatcttat ctagaatcac
     1021 ttcaaatgtt tcaaaggtat ttacatctaa ctgacataac caaagcaagt acttcttttc
     1081 tttgtaagtc caatcaatat tacggtaaga ggacattaga ttatggtacc ttgctctttg
     1141 ttcaactgaa gccaattccc agcgtttttc aaatattttt tcttcttcca ttattgatcc
     1201 ctcgcaagac gctacctcaa acttaaataa tgggcagcga aagtctaatt ccttagatct
     1261 aagtatagca aacaaatatg aaaaaaactt agaaggtagt aaaagttctt ccgtcttttg
     1321 ctttgcttct attcttaatg tgtttcgtta tcggtgccct acaaagattt tcattaatta
     1381 cacactcatt tattgttgtg tattttctga catttctctc ttgatcaatt attttaattc
     1441 catgggcatc ctgatagttc ataggagagg agcggctatc agaaatagtt gcacctatct
     1501 cttgaacttc ttttacaact tctggaaaat ccatccatcc tgcttcagct gtcaaaagta
     1561 ataatctata atatttatct ttccttttca atctaagaaa tcgtcacccc attctttttc
     1621 ttctattata aactatttaa cttttagaat tcaaaaaccg tttgtctatt tagagtttac
     1681 cctaaataga caaaaatata acattgaaaa atttagagtt ctaacgtatg attagggtgt
     1741 attataaacg ttatagaaaa gtagggaata aatttgaaaa ttgcttatat tagagtttca
     1801 tccatcgatc aaaatgaaca acgtcaaatt gaagaaatga aaaagtttgg cgctgaaaaa
     1861 atttttatcg aaaaacaatc tggagcgacg attagtcatc ggccgatttt ccaggaagct
     1921 ttagattttg tcagagagca agatattttt attgtagaag ccattgatcg acttggacgc
     1981 aattatgacg agattatcga ttcagtgaat tatctaaaga agaagaatgt gcgacttatt
     2041 atcaccagtt taccgatcat ggctgaagca attggaaatc ctctcctaga taaattcatc
     2101 aaagacttaa tcatttaaat ctcagccatg attgctgaac aagaacgtac tgaatcgaaa
     2161 cgacgccagg cacaaggaat taaaatcgct aaagcaaacg gcgtctataa aggtcgtcct
     2221 aagttataca gtgcagacac caaagatcct caacgtcgcc ttgtctatag gagtatcgtt
     2281 caggatcttg agaatggggt tgctatctca aagattgcaa cagactacaa tgtgactagg
     2341 caaacaattt accgaataaa aaaagaaatc gaccaactaa ttgtatgatt tcttttttga
     2401 atcacgtaat tattaattag ctttgtcatc aatacagaca atcattttag attcatctag
     2461 ctaagttgat tacatttatg ttaaaaagaa tttagttaac ataaatataa ttgatgcata
     2521 aatcgtcctt atttaaaggc atttaacatt gacaacttag tataatgtat cttatgtaaa
     2581 ctaataagat ttgaatttta ttttatacac actcgataat agatcaattt tatggtcccc
     2641 cgcatttacg attgcacaag agaaacataa agtcgaccta ttagcatttc ttttcaattc
     2701 aaacattaaa aaagtttata cctctgctac taaaaaatga aattatctat ttaaaattta
     2761 aaacggtaaa tctgattcat ccaaattgga aaatggatca gcctgtattg gagctttgta
     2821 tttctccata ataacagata tctttttttc attttttaaa aaataccttt taaatttctt
     2881 atagtcctct gaaatcaaaa tatagcgtga tggatactca tcaaaagaag aatcgtattc
     2941 ttcctttaaa attcccgatt tacttaattt ttctagtaca tcaaatactc tatcactcaa
     3001 gtaactcctt aaattgttat cactttccca ttgaactact tttctataag ttgaattatc
     3061 agttgaaaag atagaatttt catccataaa cgcatatatt agcagcaatc tttcatcatt
     3121 gtcaaattct attggatcaa aaatatctaa gttgtcatca aaatctttat cagttccggt
     3181 aacttttttg ttaattactg aaggtgtctt gtaagaaata gaatctaaat caaagcgttt
     3241 tgattctaaa ttattttgtg ctttctcaat taaataattc actttttttg tgagaaacct
     3301 tctgatattg tctttgctat atgttccata aaaaccctta tcttctaaag agtccctgta
     3361 cttcttaact tctatatact cttcagccaa aacttgtgga actggttcat ctaagaaaaa
     3421 aacccctacc tcgcctttgc tatttttata aaagtaatca atttctgcta tagttcccga
     3481 tctataatta tcaactggag aacccaactt atggccaaaa atcgctaata atatatcact
     3541 attttctaca atttgtttat taataacact ttgcccagag ctattattta tataagtagg
     3601 aatcgcattc gtttcccatc gaattggcat caaaattact tttgtattat ctgtattttc
     3661 tatattccat ctatatatgg tttcttctac ttcattcctc tttgctgcta tatcacctgg
     3721 tgatgctata aatactttta ttagatttat ggttttcatt tattgtacct ttctctttat
     3781 gtctctttaa agtaattttt gtataaaatt tattgacttt tacactatag gacaactttt
     3841 atctatttag tacaaaaagg cccactaaac taattgatta gttaggagcc tattacaggt
     3901 atattttcaa ctcgctttta agcaaattct actaccagct ttttgcctaa tgccctagcg
     3961 atatcatcaa gtttatcgaa agatacgtta tcccctctct caattcgagc aatagtagat
     4021 tgagttgtat gtgctttttg tgcaagctcc gcttgagtta ggcctgcttc ctcccgtgca
     4081 tgataaagag caaccgcagt ttcgagtttt cttccttcac ggtcatatgc ttctgcaaaa
     4141 tctttgtctt tttgcttttc ttcaatcaat tttttaatca tcatttgtga accactcctc
     4201 tctaagttct tttgcatgtt taagttcact tattggtgtt ttctccgttt ttttagtaaa
     4261 tccatgggta atgacatacc ttccattgac cacatggaaa tagatagctc tttggatgtt
     4321 tgatcctacc ttacttctta attcgtacaa attagtgtct aattttttca cccatttcaa
     4381 ccgttgagct actagcatcc cattcttttc tgtttcttct attgtacgta aaagcttcgc
     4441 actatctttt tttggtaatg tttccaacca ttctaaaaat tcatcatgac cattaggtct
     4501 tttaaatgtt tcaaaagata ttttttccat acccaattat atcatatatt gattttatat
     4561 tatagtatat aacataaaaa ttcgaat
//
LOCUS       DACGLW010000134         4424 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.142, whole genome shotgun sequence.
ACCESSION   DACGLW010000134 DACGLW010000000
VERSION     DACGLW010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.142"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            690..1658
                     /locus_tag="IXT53_002795"
     CDS             690..1658
                     /locus_tag="IXT53_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HAQ4906555.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            1669..2484
                     /locus_tag="IXT53_002796"
     CDS             1669..2484
                     /locus_tag="IXT53_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HAQ4906556.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2552..3262
                     /locus_tag="IXT53_002797"
     CDS             2552..3262
                     /locus_tag="IXT53_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HAQ4906557.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            3298..>4424
                     /locus_tag="IXT53_002798"
     CDS             3298..>4424
                     /locus_tag="IXT53_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAQ4906558.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIK"
BASE COUNT         1490 a          661 c          921 g         1352 t
ORIGIN      
        1 atacaaataa ttcaccaaaa aggtgagtaa tgccattttt gaaaattgag cttgttttat
       61 gtctacatct gtatccgtat ataatccaaa ggattataaa ctcagaagag tctttatttt
      121 ttctgaatat aactactgta tcaaaaacaa tcgattatga gacaaaacaa aatgacctct
      181 tttatttaag aaaggaggtc attttttgta tcataaattg tattaaaatc aatgtatcac
      241 tttttaatca gttttaagac cataaattgc gtactaaaag aaattctgca attcaaaata
      301 aagcatttta agccattttc aaatttaagt gcacccatct acccgtcaaa tcactttatt
      361 ttccactcca gtagtttcct aattcaactg tgtgcaaaaa cgatttcttc taaacaaatc
      421 aagacaaact tataaacctt catcccagca ccataattat ttaaatcata ggcatttttc
      481 gaaattagta tatgtactca agtttcactg cagattattt atatttttta gagaaataaa
      541 tttttcctat tgacaaacac aaagaaagcg gatacaatgc aattatgtca catgacgtta
      601 tatgacgtta tgtgatatct ttacatttaa tcagttactt ttattttgga taaaattaac
      661 atttttaaaa ttttaaggag gaaatgttga tggatgtagc aaaaacggtt aacgcaattg
      721 tagaaaaaaa tgatggagag atttctggaa tttatttggt ggcttgtggc ggttcgctgg
      781 tagatatgta tgtctcagat tattttctta aagccgaagc atcgaagtgt attacaggct
      841 tatatacagc gaacgaattt aaccatgtaa ccccaaagca attagatgat aaatctgttg
      901 tcattctatg ttctcatgga ggaaatacag cagaaacggt aaaagcagga gaagtcgctc
      961 aagctagagg agctgtgact gttggtctga ctcataataa aactgcggat ttattaaagg
     1021 tttctgatca tagtttcctt tacgaatggg gagatgatgc aaaaatagtc aataatccta
     1081 tggccatcat tcttgattta actgtatctt tggtcaatgt taccgaaaca tttgaacatt
     1141 acggtaaatt cagagaaggc atgaaggtta tcgataccgt aattgaaaat ggcattaagc
     1201 aggtgcagga tcgtattaaa gtgtttgctg ataagtacaa agatgaaaca atgttttata
     1261 tcttaggtag tggaccatca tatggacatg cttatggttt ttcaatttgc tctttgatgg
     1321 aaatgcaatg gcttgattca acttctgttc attctggaga attcttccat ggaccttttg
     1381 aagtaacaga caaagatacg aattttattc tcttgatgag cattggacgc actcgtgctt
     1441 tagatgaacg ggcaaaacga ttccttgata aatatgcaga aagagtagaa atcgttgatg
     1501 caaaggaact aggattggat atgatatcag atgaagtttc tgaattcttc aatcccattt
     1561 tattctacag tattctttca gaatatcgtt cagctttagc agatgtacgc aatcatgatt
     1621 tagatgttcg tcgctatatg gggaaagtgg agtactagga ggattaaaat gaaggtatta
     1681 ggcttaggtg ataacgtcgt cgataaatat gaaaatctgg gaattatgta tcctggagga
     1741 aattcattga attttgcagt ttttgcaaaa aaactgggac atgaaagtag ctttatggga
     1801 gtttttggct cagatgagga ggcagctcat gttttatcag caataaaaga aataggactg
     1861 gacaacagtc actctcgctt tgaaaaaggc gagaatggct gtgccagagt gaagattgat
     1921 gagggcgacc ggattttcct tggcagcaat tcgggaggtg ttactcgtga atacccgatt
     1981 cagcttactg aagaagacag agaatattta aaccagtttg aattgattca tatgggatta
     2041 tatagccatg tcaatcatct attagaagag ttacaaaatg tttctggaaa gatttcttat
     2101 gatttttctg acgactttac ggaagaagag gttcagcggg caattgataa agttgatttt
     2161 ggcttctttt ctatagaaga cttttccgat gaagaagtaa agcagttttt gatgaagcat
     2221 ttttgtgagc gaaatgatgt attgattgca acccgaggag aaaaaacggc tattggttat
     2281 gatggagcaa agttttatga agtgaacccc gtgctagaag aaccagttga tacaatggca
     2341 gcgggagatt ctttcttaac agctttccta ctttattttc tcgaagggga cgatatagtc
     2401 accgcgatga aaaaggggaa tgaatttgct gggaagtctt gtctggttga cggttcgttc
     2461 ggctttggta ttcattacga ttgagcttgt tttaaattcc tgcatttgat aacctaaaga
     2521 aagaaatccc ggtaaagtgg aggttaaaaa aatgctggat acaaatgcac aattaccatt
     2581 gtacttacaa ttaaaagaaa tattaaaaaa taaaatcaat acaggggagt atcccgagaa
     2641 tacgaaaatt cccgctgaat cagagctgat agaaagttat gaggtcagcc gaatcacagt
     2701 gagaaaagca gtagaagaac tggtagaaga agggtaccta cttaaaaaac aagggaaagg
     2761 aacttttgtc aaccggcata aagtttttcg taaaattgaa tatattatgg gtttcagtga
     2821 gagttgtaaa tctaatggtt ttacaccgac tagcattgtt ttggaacgca agatcattcc
     2881 agcaactgaa gggatggcaa aaaaactcga tatcgctcaa ggtgatgatg ttttatatat
     2941 ccagagaaag cgaatggcgg ataacatccc cattatgtta gaaaacaatt atttttctta
     3001 tgaaagatat agtttcttaa tggaggaaga gctgactgat tctttgtatg aagtacttaa
     3061 agtccaaggt accttagcca tcaatcctgg ggaaactgca cttgaaattg tcacagctga
     3121 tgaccaaatt gccaaaataa tggaagtagc tgttgggacg ccctttttct ttatggatac
     3181 tttaatcaac gatcaaaaca acataccaat tcatgtaggt aatcaatatt acctgggaga
     3241 agtatatcgt ttttacttat gatttcctgt ttggatttca aggagtagat tttatttatg
     3301 aagcaaatat tgcggtcaaa aatgattttt gatggagagc agccaccttt caaaggttat
     3361 attgtaatca atgaagggat cattgaaaag gtggaaaaca actggaatta cagtgatcta
     3421 ttaaatgaaa acacggaatt aattgcttat gatgatcaat tcgttatgcc agcaattcat
     3481 gataatcatg tatttttcag tggttatttg gctatgaatg ctggcataga tttaagcgat
     3541 acatcttcag ctaacgaggc tgtagagcgt attaaagctg taatgaacca taaaaaagca
     3601 gatgaaccaa tttatgctca tggctgggat gctgccctgt ggaaaactga cccatcagaa
     3661 gagctattga atgaaataaa ccactttggt ccaatcacag cgatcgacaa aaatcgcagc
     3721 cattgttgga tgaatgctat tgctaagcag acttatggtt ttacagaaaa agaaaccagt
     3781 gctgaaagtc gagtattatt aattaaggaa atgctagcaa acaaaaagct tttagcagag
     3841 gtttacgctg aatttgaagc attactgctt tcaaaaggtg ttatatcaat gaaagaaatc
     3901 gtttttgatg acgcagaatt tctttctaat atacctcaga ggaaaatact tagtaaccta
     3961 tatgtgcagg cagtccaaga acctctacag catgaacttc tatatgagta taaggaaaaa
     4021 gttttccctg aaaaggtccg ctttggtgga gtgaagatca tggtagatgg tgttgttgcg
     4081 gatagaagtg gagatattta cggtgtttat cagtcaggag tagcttcacc cgagattgac
     4141 tatgaggcaa ttaaagcaga agttgctgca ttcaatgaag tagagattcc ttgctgtttg
     4201 acaacggaag gagataaagc gggttataaa gctgcgtcaa ttcttgtcga atttggaaag
     4261 cggttacctg aaggcgtttt taattcgatt agtgatttgg aaatggttac tcaaaagaca
     4321 gcagaaaaaa tgaacgaagg acaagtagta gcagaggtat atccacaaat tctcggcttg
     4381 aatcaaacgt tagaagaggc ttacatgtca actgttataa aaaa
//
LOCUS       DACGLW010000135         4416 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.143, whole genome shotgun sequence.
ACCESSION   DACGLW010000135 DACGLW010000000
VERSION     DACGLW010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4416
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.143"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            59..913
                     /locus_tag="IXT53_002799"
     CDS             59..913
                     /locus_tag="IXT53_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003065868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ4906559.1"
                     /translation="MENSIYLVTEPNTQLKDCYFNFCGYSKTEPRHSFGPAIREQYLI
                     HIVLEGEGYYSINDQKYFLKKGQGFVIPPGVSTFYQADEKNPWSYVWLGLSGNLVEYY
                     LKEIGVGKNHLTFKVENLNSFKGLIFETLSYDQDNLVSELSRQKQVYKFLELLLNSAS
                     YLNEGIITKKMNPFVRQTIEIIRENAYQKLSVKDIAVSLSIHENYLSRLFKNDMGISI
                     KEYISEIKISIANDLLSSTEYSIQQISDKIGFSTVQSFSKAFKKANGVSPGVYRKRRL
                     GLGIINGE"
     gene            1010..3259
                     /locus_tag="IXT53_002800"
     CDS             1010..3259
                     /locus_tag="IXT53_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HAQ4906560.1"
                     /translation="MYKDNEMNIIYRSENQQFHLFNQEISYIFKIPEDGKLLHLYYGE
                     NLPDNDYSHLIEMHHRPMTTYRNENDLLYSLEHLKQEFPEYGTTDFRHPAISLLQKNG
                     SRLSDFVYQGYEVEEGKPKLEGLPATYVDNNDESKTLKVYILDSVTNVQVELLYTIYR
                     DYPVITRSARVMNKGKDIQIIENISSLSLDLPDANYEWQQLSGAWGRERTIKSRTLQQ
                     GIQSIESTRGISSHQHNPFVTLKRKNTDENQGEAIGAALVYSGNFLIQAEVDTWDVTR
                     LQVGINPFGFNWKLNPGEIFTAPEAILVYSSKGLNAMSQTFHQLFRKRLARGKWREKD
                     RPVLINNWEATYFDFDEEKLVNIAKKAFDVGIELFVLDDGWFGERENDCAGLGDWHVN
                     PKRLPRGIGSLSEKIRSFGMKFGLWFEPEMVNKDSELYRTHPNWIISTPNRKPNHGRN
                     QYVLNFGLDEVVENIFDQMCKIIDESNLDYIKWDMNRPLTDVFDSHLSADQQGEVFHR
                     YVLGVYRLYEKLITKYPNILFESCSSGGGRFDPGMLHYAPQAWASDDSDAIERLKIQY
                     GTSMLYPLSSIGAHVSIVPNHQTNRITPIKTRGNVAFFGAFGYELDLNELSEGDLRIV
                     KEQIAFYKKYRTIIHNGTFYRLLSPFDDDNQTAWMVVSHDKKTAIVAHYKTLNEVNAP
                     YRRLKLKGLDNDGLYNVGQNSLRSGMELMRAGLVCSDASSGQINDQNRRPETFDFDSK
                     IWVITQEEK"
     gene            3262..>4416
                     /locus_tag="IXT53_002801"
     CDS             3262..>4416
                     /locus_tag="IXT53_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HAQ4906561.1"
                     /translation="MNNQKSLLQKGAILSISLMLTSSQAINGVIPQMKQSLGISQSQS
                     ELLGTAPSITVILFILLSSYFAKRIGMKKTIILGLLLAGIGGIIPVFTATFQTVLISR
                     VILGAGLGVYNSLAVTYISSLYSGNTRATLLGIRNSMEALGQTILIFLAGVLVNINWT
                     ASFLVYAIAFPVALLFALKVPEIRDENSDIPENHVKEKMNPMVFALVLFAILLVMNSI
                     AISVRFASIATEIKGVNFNASNYLALMPILGILAGFMFGPINKWIGKGTLYLGIIFFI
                     ISNLLIAVSNGNMTFLLTGLFLSSITGSWCFPFIFSNLDKVTTKNTINFATSLIFIGC
                     NIGNFIAPIAMQLAQFLTRSTNLTAPFFVFAGIFIVILFVTFFATNKKNTR"
BASE COUNT         1556 a          626 c          806 g         1428 t
ORIGIN      
        1 gtaataatat aaacataata aaacaaaaat tatccaaatc taacatagga gggtggatat
       61 ggaaaattct atatatttag taacagaacc taacactcag ctgaaagatt gttactttaa
      121 tttttgtggg tattcaaaaa cagaacctag acatagtttt ggaccagcta ttcgagaaca
      181 atacttaata cacatagtgc tagaaggtga aggctattat tcaattaatg atcaaaaata
      241 ttttctgaag aaaggccaag gatttgttat cccaccagga gtatctactt tttatcaagc
      301 agatgaaaaa aatccgtggt catatgtatg gttgggttta agtggcaatc ttgtagaata
      361 ctatctaaaa gaaattggtg ttggaaagaa tcatcttacc tttaaagtcg aaaatttaaa
      421 ttcatttaaa ggattaattt ttgaaacttt atcgtatgat caggacaatc tagtcagtga
      481 attgtccaga caaaaacaag tttataaatt tttagaactc ctcttaaatt cagctagtta
      541 tctgaacgaa gggataatta ctaaaaagat gaatcctttt gttcgtcaaa caattgaaat
      601 aatacgtgag aatgcgtatc aaaaattatc tgttaaagat attgctgtca gtctatcgat
      661 acacgaaaat tatttatcac gattgtttaa gaatgatatg ggaatatcaa taaaagagta
      721 tattagtgaa atcaaaattt ctattgcaaa cgatctgtta tcatcaactg aatattcaat
      781 ccagcaaatc agtgacaaga taggattctc aacagtacaa tctttttcta aagcctttaa
      841 gaaagcaaat ggtgtttcac cgggagtata tcgaaaacgt agattaggtc taggaatcat
      901 taatggtgaa taaactaaac atttgatttt gataaatatt cgttacttta aacgcttact
      961 atttatgtga gcgtttttta taatgcaata aagaaacgga ggagagctta tgtataagga
     1021 taatgaaatg aatattattt atcgatcaga aaaccaacaa tttcatttgt ttaatcagga
     1081 gataagttac atttttaaga taccggaaga tggtaaatta ttgcatttat attatggtga
     1141 gaacttacca gataatgact atagtcatct aattgagatg catcatcggc ctatgacaac
     1201 ctatagaaat gaaaacgatt tgctatattc gttagagcac ttaaaacaag aatttcctga
     1261 atatggtaca acagatttta gacatcctgc tatttcatta ctacagaaaa atggctctcg
     1321 cttatcagat tttgtctatc aagggtacga agtggaagaa ggaaaaccta aactagaagg
     1381 tttacctgct acctatgttg ataataatga tgaaagcaaa acattgaaag tttatatttt
     1441 agattcagtt acaaatgttc aagtagaatt attatataca atttatagag attatcctgt
     1501 tattactcgt agcgcgcgag ttatgaataa aggaaaagat attcaaataa tcgagaatat
     1561 tagcagttta tcattggatt tacccgatgc aaattatgaa tggcaacaat tatctggtgc
     1621 ttggggtaga gaaagaacta ttaagtcgcg tactttacaa caaggaattc aatcaatcga
     1681 atctactcga ggtatttcaa gccatcagca taacccattt gttactttaa aaagaaaaaa
     1741 tactgatgaa aatcaaggag aagctattgg tgcagcacta gtttactcag gaaacttctt
     1801 aattcaagca gaagtagata catgggatgt tactagacta caagttggca tcaatccatt
     1861 tggatttaat tggaaactca atccaggaga aatatttaca gcaccagaag cgattctggt
     1921 ttattcaagt aagggtttaa atgccatgag tcaaactttt catcaactat ttagaaaacg
     1981 cttagctcgt ggtaagtggc gagaaaaaga ccgtcctgtc ttaattaata attgggaagc
     2041 aacatatttt gattttgacg aagaaaagtt ggtaaatatt gctaaaaaag catttgatgt
     2101 tggtattgaa ttatttgtat tagatgatgg ctggtttgga gaaagagaaa acgattgtgc
     2161 aggattagga gattggcatg taaatcctaa aagactacct agaggaatcg gcagcctttc
     2221 agaaaaaatt cgtagttttg gtatgaaatt tggtttatgg tttgaacctg aaatggtgaa
     2281 taaggatagt gagttatatc gcacacatcc gaattggatt atttctacac ctaatcgaaa
     2341 acctaatcat ggtaggaacc aatatgtatt aaattttggg ttagatgagg ttgttgaaaa
     2401 tattttcgat caaatgtgta aaatcattga tgaatcaaac ttagattaca ttaagtggga
     2461 tatgaatcgt ccattgactg atgtatttga cagtcacttg tcagcagatc aacaaggaga
     2521 agtattccac agatatgtac taggcgtata tcgcttatat gaaaaactta taacaaaata
     2581 tcctaacata ctatttgagt cttgttcatc aggtggtggg cgctttgatc ctggaatgct
     2641 tcactatgca cctcaagctt gggctagtga tgattcagat gcaattgaaa ggttaaaaat
     2701 tcaatatgga actagtatgc tgtatcctct ttcgtctatt ggtgctcatg tatcaattgt
     2761 tccaaatcat caaacaaacc gtataacacc tataaaaact agaggaaatg tagctttttt
     2821 tggtgcattt ggatatgagc ttgacttaaa tgaattaagt gaaggcgatt tgaggattgt
     2881 aaaagaacaa attgcctttt ataaaaaata tagaaccata attcataatg gtacttttta
     2941 tagactatta tcaccattcg atgatgataa tcaaaccgcg tggatggttg ttagtcatga
     3001 taagaaaacg gctattgttg ctcattataa aacattaaat gaggtaaatg ctccttaccg
     3061 ccgactgaaa ttaaaagggt tagataatga cggactttat aatgtaggac aaaatagttt
     3121 gcgatctgga atggaattga tgagagcagg actagtctgt tcagatgcat ctagtggaca
     3181 aataaatgat cagaatcgac gcccagaaac atttgatttt gattccaaga tttgggttat
     3241 aacacaggag gaaaagtaga gatgaataac caaaaaagct tacttcaaaa aggagcaatc
     3301 ttaagtattt cactaatgtt aacaagctct caagcaatta atggtgttat tccacaaatg
     3361 aaacagtctt tggggatatc acaaagtcaa agtgaacttt taggaactgc accaagtata
     3421 actgttattt tgtttatctt attatctagt tactttgcca aaagaatagg aatgaaaaag
     3481 acaataattt taggtttatt attagctggt ataggaggaa ttattccagt atttacagct
     3541 accttccaaa ccgttcttat tagtcgagta atattaggtg ccggtttagg agtatataat
     3601 tcattggctg tgacatatat cagctcttta tactctggca atactagagc aacgctatta
     3661 ggtattagaa attcaatgga agctttaggt cagacaatcc ttatttttct tgctggagtt
     3721 ttagtgaaca ttaattggac ggcttcgttt ttagtctatg caattgcgtt tcctgtagct
     3781 ctcttattcg cgctaaaagt tcctgaaatt agagatgaaa atagtgacat acctgaaaat
     3841 catgtaaaag aaaagatgaa tccaatggtt tttgccctag ttctatttgc aatcttactc
     3901 gtaatgaata gtattgctat ttctgtacga tttgcatcaa ttgcaactga aattaaaggg
     3961 gtaaacttta atgctagtaa ctatttagct ctaatgccta tcttaggaat actagcagga
     4021 tttatgtttg gaccaataaa taagtggata ggaaaaggaa ccctatactt gggaattata
     4081 ttctttataa tttcaaattt gttaattgct gtgtcaaacg gtaatatgac gttcctgcta
     4141 acgggtttat ttttatcaag tataacagga tcatggtgct tcccatttat ctttagtaac
     4201 ttagataaag tcactacaaa gaatacaatt aattttgcta cttcattgat ttttattggc
     4261 tgtaatattg gtaattttat agctcctatt gctatgcaat tagcacaatt tctaacaaga
     4321 agtacaaatt tgactgctcc attttttgta ttcgcaggaa tatttattgt gatcctattt
     4381 gtaacatttt ttgcgacaaa taaaaaaaat actaga
//
LOCUS       DACGLW010000136         4282 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.144, whole genome shotgun sequence.
ACCESSION   DACGLW010000136 DACGLW010000000
VERSION     DACGLW010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.144"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            complement(37..1257)
                     /locus_tag="IXT53_002802"
     CDS             complement(37..1257)
                     /locus_tag="IXT53_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ4906562.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1277..1882)
                     /locus_tag="IXT53_002803"
     CDS             complement(1277..1882)
                     /locus_tag="IXT53_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005234653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HAQ4906563.1"
                     /translation="MKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELPLF
                     NKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVDKP
                     VMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLIDEE
                     QVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1931..2863)
                     /locus_tag="IXT53_002804"
     CDS             complement(1931..2863)
                     /locus_tag="IXT53_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HAQ4906564.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3284..4144)
                     /locus_tag="IXT53_002805"
     CDS             complement(3284..4144)
                     /locus_tag="IXT53_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ4906565.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1219 a          861 c          701 g         1501 t
ORIGIN      
        1 tttattcttt atttaaaact ttgcaacaga acctttttat tttacagaag cactcgaaac
       61 tgcatctact gcagtttcat cgtgtgtgct ggcgctggtt actgcatctg tttttccacc
      121 atgaacagat gcacttgaaa ccgcatcttc atttccaaca agttgttgac ccgtttgttg
      181 taaataccac tcaatgattg gtttgaaatc aagaatgtat tcattgattc cagcatactc
      241 tccattttca tcatgcattg cttggtaatt atgcacaata tatttttctg gcacttgttt
      301 tggtacatga acacgtaccg catctgttgc tccgcttcgc agttgttgaa ttacccactc
      361 tacattttta tacgattttt ctgggtgaca gttggctaat ggattcccta cctgaccggg
      421 cacacgagca gctaacatct ctttcgcttc ttggcgcaaa ttataatata aaaattggtt
      481 attattatct gcaaaagtta attccatcgg catcgaatct aaaaagtaat tgatttgatc
      541 cactgtcaat aacccacgat ttaatttgac gtaagtattt ccagaaacgg caccgactgc
      601 ttcagaagct ttttccaacc aatgatcatc tgacatatca acatcgggta ttgtcgtagc
      661 aattgttcct gtcgcatgca aatcttctgg tggaagcttt ttaggccctg ctaaggtaga
      721 cacgacttta atgaatttta caaaacttgc taatgtactg cctaaaaagt caacagttcg
      781 tggatcaatc aagcgatctt cttggtcaaa tgcttctttt gcttttccca ataaaaattc
      841 atttccgggc atcactaatg cattcacgcc tggtgcgtcc aaaatttggc gcaagtgtaa
      901 ttgcgcgcgt gaagttcctt gatcgtagta agaagcaccc acaaccatta caggtttatt
      961 ttcaagtgga tggattttaa atgatagcca ttcaagtgca cttttcaatc ctgctggaac
     1021 cgtatgattg tgttctggcg tagcaataat taccccatca gccattaaga ttttgcgatt
     1081 tagctgttga atagcaggac tatttgtttg atcatcatct tgattgaaca gaggtaaatc
     1141 tttgatttca atgatttcta aattaaatag ttcatgatat tgtttttgga taaatgttaa
     1201 taatttacgg ttatatgaaa gttcagctgc tgatccgacg atacctatta atttcatctt
     1261 tgtttgctcc cctcgattaa atcgtttccc atgaaaagct ttcggcttca cgtttatttg
     1321 cttggtgcgc atgttgcaat tgactagtga ttgtgacaaa gagtaagaaa tctttgaata
     1381 gttcacgtaa ttttgcgact tgttcttcat caatgagtcg tccttgctca tcaaaggctt
     1441 ggagtgaatg acccaataaa aattctgagc ttggcataat tcgtgccttt aactctggtg
     1501 aatccaatat ttggcgcaaa tgtgcttgtg cgcgtgaaga acctaacgtg ccataagacg
     1561 ctccggtaat catcactggt ttatccacaa acggaaaaat accatatgaa agccaattta
     1621 atgcgttaat caatgaagca ggaaccgcat ggtcatactc tggtgtgctg atgattacgc
     1681 catctgcttg ttcaattttt ccagcaattt ctttaacaac atttggtagt tctgtgtttt
     1741 caggtttgtt aaatagtggc agctccttga tttcgacaat ttcgatttct gcctcattta
     1801 caaaataatt tttcataaac tgtaataatt cacggtttgt tgaccgcgtt gaatttgttc
     1861 ctactaatcc aataaatttc atcttcattc tctttccttt ctaacatcga tttctgactg
     1921 ttcgcacgta ctaaattttt tcaaatcctt ttgtatacaa gaattgattc tcttttgtta
     1981 caactgctcc ctcgataccc ggctgttctt caagcgcttt caacacttct aaagaagatg
     2041 ctccaaataa acgtgtagtc cagatttctc catctacaga agaatcagac aaaatcgtca
     2101 ggcttgctac gtcattttta actggaaagc cagttttcgg atcaatgatg tgatgataca
     2161 ctgacgaacc attttgtaga taacgttcgt agattccaga ggtaactaaa gattggttgt
     2221 ttagtttaac caccgcaagc gactcttcac gtgaaccttt aggatttcgc aaaccaatac
     2281 gccaagcacc atccgaacgt cttggtgatt caccaaaaac aacgatattt cctcctaagt
     2341 taactaaagc aaacgctgca tttcgctctc ggcaatgtgt aattaattta tctgcaatgt
     2401 aaccttttgc taacgccccc aagtcaatca tcattcctgg tttggttaaa aaaacggttt
     2461 tcattttttc atctaattga atttggtgtg gatccgttag cagaagtaac tcagttatct
     2521 ctgcttttgt tggaaccaat gcatcttcaa atccaatgtg ccatttttgt acaaccggac
     2581 caatagcaat attcagataa ctatttcttg cacaactatg tgcttttcct aatttgatga
     2641 gctcaaaaag ttcaggatgt acaatcacag cttgtcgtcc agcagcttga ttgatggcca
     2701 ttaattcaga agttgcatca tgtgcgctga agcgatgttc ataaacgtgc aaccattcgc
     2761 ctaactctgc tagtaatcga ctaggctctt catgcccaac ttgtaatgta attgttgtcc
     2821 ccatcatttg ccagattttc gtttcttcga tcacctgatt cattttgtac cttctttact
     2881 tgaactacgg attacatgag taattagttt ttatttttgt tactgttttc acaaccagat
     2941 gatacgcctg ctagatttat ttgtcaaccg attttatata tttttttata taataaggta
     3001 attttaatat aaaattttat attgtaaaaa aacaaataag ggaaacaaac aacacatttt
     3061 tgtgtgttgc ttatttccct ctaactatcg ttttctattt gatggttaac ctacttaaaa
     3121 taaatactgc aacgacaaac ggagatcaag catgctttgt aacgtatttt acgataatta
     3181 cgactttatt ttattactct atcttgctgc catacccgtc tatccctaat catctttggt
     3241 gaattttttc aaagttagca taacaaaaat aagtattttt tccttagtta atcaatagta
     3301 tcattttttt catatgattt aattcatcaa ttaatttttt gtttttatta attttagcaa
     3361 aagctagtgc atcttgaatc aattcgagca cttctgcctt cgacttattt ttagcctgtg
     3421 cgttcttcgc taattgggca gcagctcgtg ctaagtaata agtcaaatgg ttatccgaac
     3481 aaatatcgat tgcgtaattt agtagtgcat cacttgtttc caaatcaccg tcttgcgcaa
     3541 aaaaaacacc acaatgaaag acgatattta acacccgcca gatatcttct gttgtacgaa
     3601 tcggatacgt ataaattttt tcaaacactc gattgaaata aacttctgct ttttccaact
     3661 ctttttctct agcatagacc attccaatac ctgtatatgc caataaacga aacaacgtgt
     3721 tggctgtttc tgccatcaac aactgatcaa aatggaacaa cacatccgta attggtgcat
     3781 tcgtaaaaat catttcaaat ccttttagat agtagtaacg tgcaatcagc tcagactcgt
     3841 caattacctt gggatcaatg gctgctaaaa tctgtgtcaa ttgttcatat tcactcgtaa
     3901 tcagtgaaaa ctctgctttt tctagctgtt catcaagttg tgtattgatc acgttcatcg
     3961 aagaaaaaag ttcaactaac ggaagattga ttcgattaca taatttgatt aaaattttta
     4021 acgaagggac ttgaccattg ttctcaaatt tacttaatgt tgcttgtgta caaattcctt
     4081 cagacacttc tttttgagat aagttggcag cctttcttgc cgtaataaat ttttcaatgt
     4141 tcattatttt ctctccaatc agacctcaca cgaaaatcat ttctgtctcg attttagcat
     4201 gttttctttg ggaatgcttt aggttctgtt gcaaagtttt aaataaagaa taaagtccct
     4261 tacggtatct atgatttaag ct
//
LOCUS       DACGLW010000137         4170 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.145, whole genome shotgun sequence.
ACCESSION   DACGLW010000137 DACGLW010000000
VERSION     DACGLW010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4170
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.145"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            <1..749
                     /locus_tag="IXT53_002806"
     CDS             <1..749
                     /locus_tag="IXT53_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HAQ4906566.1"
                     /translation="CVIWGIINGYTSQRATYRAVCSVLFPNGDFPSRSRFTRLSSNLA
                     YTIKIIRYFFIKKLTKGELVGIIDSFPSPLCKPVRNRQAKLLNQIAKVGYNSTKKSYF
                     YGLKIHMIVTKTGFPITYSITNPGVHDVKVLETLSEEANLPNILGDKGYISHKIHEKL
                     ALMGITISIPPRKNMDKSEKLDHSLLGKQRKTVGTVFSSLEKLGCQNFNSRSVKGLES
                     RFESILLAYSVLLSRAQRRFEGTLRYSLGY"
     gene            complement(808..2610)
                     /locus_tag="IXT53_002807"
     CDS             complement(808..2610)
                     /locus_tag="IXT53_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HAQ4906567.1"
                     /translation="MGLFDFLKKKQGKVSKHVDYQLASSLFRTGKVFAEKLEVDISNV
                     DEFRKTFIAFDVETTGLNSSSDRIVEIGAVLFKEGVPIQSFGTLVNPKVRIAASASAI
                     NNITNEMLNSAPNEQEVYPQLVEFLGDALDGKITMCAHNASFDFGFLSNTFSRLGYDA
                     KIKYVDTLSLSRKHIKGIVNHKQETVAEYFGLINDTAHRAESDADICGKIFWGVLDII
                     EESLEEQKKQIEKMTPDIKELEACAFIQDIIEKKGCDTSWIRYRKNSNNYVDVTCLYT
                     FLKFKFAKKGNYIIIRKDATNGIDLPIEPCTVSEGGTTNVRVYFRSPFDLTPLSNYIF
                     SAYSDCYNSMNEYMSVSNYTKKEAEQSLSMLKSISKSAMKELLFDAKNRGYDETEVNL
                     NIEKVISREDVIINGLHNRVSLNKIRNIGNWEKGFDAGYPYWERGETARKNGKVDEAI
                     ALFDKARYNGYDAPALYDSYAKAYRKIKDYDNEILILDEGIMRKTRHDVGTLEARRDK
                     AIKLLFAKQEAERIAKEKSDFLKANKKEDAISEPSKKRGRQIIQMTDDGTIIKEFDTI
                     ASAVKEVGVNSKSIRDAANGVQKHAGGYYWKYKD"
     gene            complement(2673..3515)
                     /locus_tag="IXT53_002808"
     CDS             complement(2673..3515)
                     /locus_tag="IXT53_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906568.1"
                     /translation="MKIITINDVEYAVFAANEGTSKPQPHIIETKSGTIPEGKQLSLL
                     KEYLKQNDISPIKGATTYWCIDKVLKLDSSKEKTISETIHKQKYLSLTEENIEKQHKF
                     VGASSNYGKEGLIIHDVLNAFPLHNDLNTIAMKIAVIDVTNSTHLSQYKSRLSLYDLA
                     KVILEIPNFDDRLAKGDPQLINIIARNIGAVNMFSFASKYCTYHNVEVCGRDDYSIFD
                     GIVKNTLPHYIQGLTTNKIDTWRRSFDYEAFNECVGKLLDENNIHIPFRRRKLDHFLW
                     YANR"
     gene            complement(3531..3752)
                     /locus_tag="IXT53_002809"
     CDS             complement(3531..3752)
                     /locus_tag="IXT53_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014315041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ4906569.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGNEIKIGN"
     gene            complement(3895..>4170)
                     /locus_tag="IXT53_002810"
     CDS             complement(3895..>4170)
                     /locus_tag="IXT53_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HAQ4906570.1"
                     /translation="ELIKLANKGQDYEHLADEIDELRDKRQLLLVEDASLSGENERIN
                     ELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEEHFVISFKSGIEMEI"
BASE COUNT         1258 a          842 c          608 g         1462 t
ORIGIN      
        1 cttgtgttat atggggcata attaatggct atactagcca aagagccacg tatagagcgg
       61 tttgttccgt cttatttcct aatggtgact ttcctagtag gagtcgattc actcgcttaa
      121 gttcaaactt agcttacact atcaagatta ttcgatactt tttcatcaag aagctcacca
      181 aaggtgaact agtcggaatt atagatagtt ttcctagtcc attatgtaaa ccagttagaa
      241 atagacaagc aaaactattg aatcaaatag ccaaagttgg ctataactca acaaaaaaat
      301 cttattttta tggtcttaaa attcatatga tcgttactaa gactggcttt ccgattactt
      361 actcaatcac gaatccaggt gtccatgacg tgaaagtgtt agagacttta tcggaagaag
      421 cgaatcttcc caatatccta ggggacaaag gatatattag ccataaaatc catgaaaagc
      481 ttgcccttat gggcattact atatccattc cgccacgtaa aaacatggat aaatcagaaa
      541 aactagacca cagcttactt ggaaaacaaa gaaaaacagt tgggactgtt ttttcttcct
      601 tagaaaaatt aggttgtcaa aatttcaact cacgttctgt taaagggcta gagagtagat
      661 ttgaaagcat attactggct tacagtgttc tattaagtcg agcacaacga cgttttgaag
      721 gaactttgag atattcttta ggatattaaa attaactagc accacgggta tctattaaga
      781 agatcctagg ttatcttaga ttatctgcta atctttgtat ttccagtagt atcctccagc
      841 atgtttttga actccatttg cagcatccct aatacttttt gagttaacgc caacttcttt
      901 tacagcagaa gctatagtat cgaattcttt tattattgtt ccatcatcag tcatttgaat
      961 aatctgtctt ccacgcttct tgctaggctc actaatcgca tcctcttttt tgttcgcttt
     1021 tagaaaatca gatttttcct tagctatccg ctctgcctct tgtttagcaa ataatagctt
     1081 aattgcctta tccctacgag cctctaaagt accaacatcg tgccgtgttt tacgcataat
     1141 tccttcatca agaatgagaa tttcattatc gtaatcctta atttttcgat aagctttagc
     1201 ataagaatca tatagcgcag gagcatcata gccgttatat cgagctttat caaaaagtgc
     1261 aatagcttca tctactttgc catttttcct agcagtttct ccacgttccc aatatggata
     1321 acctgcatca aatcctttct cccaattccc tatatttcgt atcttgttta atgaaactcg
     1381 attatgcaac ccgttaataa ttacatcttc tctagatatc actttttcaa tatttaggtt
     1441 aacttcagtc tcatcatatc cacgattttt tgcatcgaat aataactctt tcatcgctga
     1501 ttttgaaata gatttcagca tactaaggct ttgctctgct tcttttttag tgtaattact
     1561 aacactcata tattcattca tagaattata acaatcgcta taagcactga aaatataatt
     1621 tgaaagaggc gttaagtcaa aggggctacg aaaatagact cgcacatttg ttgtaccgcc
     1681 ttcacttaca gtacaaggtt ctataggtaa atcaattcca tttgtagcat cttttctaat
     1741 aatgatgtaa tttcccttct tagcaaactt aaactttaag aatgtatata gacaagttac
     1801 atcgacataa ttattactgt tttttcgata cctgatccaa ctggtgtcgc atcctttttt
     1861 ctcaataata tcttgaataa aagcacaagc ttcaagttct tttatgtctg gagtcatctt
     1921 ttctatttgc tttttttgtt cttccaacga ctcctcaata atatctaaaa caccccaaaa
     1981 aatctttccg catatatctg catctgattc cgctctatgg gcagtgtcat ttattaatcc
     2041 gaaatattcc gctacagtct cttgcttatg atttacaatg ccttttatat gttttctaga
     2101 gagactcaga gtatcaacat acttaatttt agcatcatac ccaagtctag aaaatgtgtt
     2161 acttaaaaat ccaaaatcga aacttgcatt gtgtgcacac atagtgattt ttccatctag
     2221 tgcatctcct aaaaattcta ccaactgggg gtaaacttcc tgctcatttg gagcagagtt
     2281 tagcatttca ttagttatat tatttattgc cgaagctgag gctgcaattc taacttttgg
     2341 attgacaaga gttccgaatg attgaattgg aactccttct ttaaatagca ccgcaccaat
     2401 ctctacaatt ctatctgaag aagaatttag gccagttgtt tccacatcaa aagcgataaa
     2461 agttttacga aactcatcta catttgatat gtccacttcc aatttttcag caaaaacttt
     2521 accagtacga aatagactag aagccagttg ataatcaacg tgtttactaa cctttccttg
     2581 ttttttcttt agaaaatcaa ataatcccac tattatttcc tcccccatta attttatcca
     2641 gaatcacttt tacgatgcct ttttcttata ttctacctat tcgcatacca caagaagtga
     2701 tctaactttc ttcttctaaa aggaatatgt atattatttt catcaagtag ttttccaaca
     2761 cattcgttaa atgcctcgta atcaaaactt cttcgccacg tatcaatctt attcgtagtt
     2821 agtccttgga tatagtgtgg aagagtattt ttaacgatac catcaaagat ggagtaatca
     2881 tctctcccac acacttccac attatgatat gtacagtact tggatgcaaa tgagaacata
     2941 ttaactgcac caatgttcct tgcgataata tttatcaact gaggatctcc ttttgccaac
     3001 cgatcatcaa aatttggaat ctccagaatt actttagcca aatcatatag agacaacctg
     3061 ctcttatatt gagaaagatg tgttgaattt gttacatcaa ttaccgcaat cttcatcgca
     3121 atagtattta aatcattatg aagaggaaat gcattcaaaa catcatgtat tattagacct
     3181 tctttcccat aattagagct tgcacctaca aacttatgct gtttctcaat attttcttca
     3241 gttaaagata agtatttttg tttatggatt gtttcactta tagttttttc tttacttgaa
     3301 tcaagcttaa gaaccttgtc aatgcaccaa tatgttgttg ctccttttat tggggaaata
     3361 tcattctgct taagatattc ttttaataaa ctcagttgtt ttccttctgg aatagtacca
     3421 gatttcgttt ctatgatatg aggttgtggt ttggaagtac cttcattagc agcaaataca
     3481 gcatactcaa catcatttat tgttatgatt ttcaatacaa attacacctc ctaattacct
     3541 atctttattt cattgccatc aggcaatata aaagcctgca caaacttgac acctagcaca
     3601 tcagcaatcg ctttcaactc atctaaggtt accgtttctc gttgtaattt cttattgaaa
     3661 ttctgtggag tctgtccaat acgtctagca agttcggaaa cacttatatt catttgctca
     3721 cacagttctt taatcatttc tgccgtagtc atacagcacc tccaagttta cgctaagaat
     3781 attataaatc atttggttta taataacaat agttattaaa atattattat aatcagcaag
     3841 caaaagaaaa cccacgactc tactgtagaa tcatgggcta tttacttctg gttttcatat
     3901 ttccatttcg atgccagatt tgaaggatat gacgaagtgt tcttcataga ctgtaacgct
     3961 ctggattatc ttccttacaa gcttatcatc gtactctaag gtacggaatt tgttcttgcg
     4021 gataaattca atcagctcat tgattcgctc gttctcgcca ctgagggagg catcttctac
     4081 taaaaggagc tgtcgcttgt ctctcagctc atcaatctca tctgctagat gttcataatc
     4141 ttgaccctta ttagccagct tgatgagttc
//
LOCUS       DACGLW010000138         4098 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.146, whole genome shotgun sequence.
ACCESSION   DACGLW010000138 DACGLW010000000
VERSION     DACGLW010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4098
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.146"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            948..1421
                     /locus_tag="IXT53_002811"
     CDS             948..1421
                     /locus_tag="IXT53_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HAQ4906571.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1430..1657
                     /locus_tag="IXT53_002812"
     CDS             1430..1657
                     /locus_tag="IXT53_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ4906572.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            2293..2664
                     /locus_tag="IXT53_002813"
     CDS             2293..2664
                     /locus_tag="IXT53_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906573.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(2920..3162)
                     /locus_tag="IXT53_002814"
     CDS             complement(2920..3162)
                     /locus_tag="IXT53_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HAQ4906574.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(3194..4096)
                     /locus_tag="IXT53_002815"
     CDS             complement(3194..4096)
                     /locus_tag="IXT53_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HAQ4906575.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
BASE COUNT         1187 a          724 c          639 g         1548 t
ORIGIN      
        1 tttcggagaa ttaaaaaata caataaataa ctttagttac tttaatgcgt tcttacttta
       61 tttttatatt atcatttttc ttattcactt ctttggttta tactattctt ataaataaaa
      121 acgaggtatg tcctatgatg aaaagattgt actattcatt gatcataacg attggctatt
      181 tgattgtatc aaatctagga aatatggtat ttggtatcag taaagaattc agttggacta
      241 ctacattatg ggaatctcta ttctttttca tcttcgtatt tttgttacaa aattatcgaa
      301 aaaaataaac atgtgccatc tatctgttgg cacatgtttt aggatttatg gtacttttta
      361 ttcggcaatc cattttattt ttctacttct tcttaatacc tctaacactt ttgatttata
      421 aactaatcca atgcatctac ttgcgcttat taagctattt cagctagata gtaccacccc
      481 atgcgtcaat gcttgatttt ccttttgtat aacattgtat aagcaataaa ggacaaataa
      541 gtggtgatta caccaaacca ttgaataaga aaaattctga tacgtaaata aacatgaact
      601 tgaaaaaatt aatgaatcgg ttagctacta cgtagttgtc ttttttctca tacagttctg
      661 ttaacttaca cttctcttcg ctagataata aaaaatcctc caactgtatt gtccaatata
      721 atcccaggca tctaaactag cattatagaa gagacacaca tagaatctaa aataactggt
      781 tttaaatgtt tcttacaatc aaaaaaagct gttaaatgca ctccacacct tcatactcta
      841 tcatttttta taaacgatat atttttattg tttttcgata aaaaatagtt gttttacaat
      901 aattattctg ctatatttta gctatcaaag ataaaagagg gataaaaatg aaatttaaaa
      961 cattgctatt aaaatttgtc gttttaggta taagtgccgt atttattttg ttcggcgtac
     1021 ttttttatac tcagatcgct acaagcgaaa aaggattcaa ttttgattgg cagaccacat
     1081 tatttgtttc tgtcatttac tttgcccttc tcctagggat cgttattgct tatttcttat
     1141 tgaaactatt gaaaaaaatc gaagataacc aagctttttc cactcaaagc ttaaacatac
     1201 taaaaaaaat cgaacgaacc attttgttta ttggtttcgc cttttttggc ttacttccta
     1261 aatttatgaa agtagcggat ttagacgatg ctccaggttt gatgttggta ggaatcgctg
     1321 tcgtattcat tccttttgca gtatacacat taacaagcgt gctggaaaaa ctgcttgaac
     1381 atgcgatttt acttaagaca gatcatgatt tgacagtgtg aggtgcgaca tggcaattat
     1441 cgttaattta gatgtagtat tagctcaaaa aaaaatgagt gtgactgaat tgagtgagaa
     1501 agtgggcatt acgatggcaa acatctctat tctaaaaaat ggaaaagcaa aagccatccg
     1561 tttttccact ctcgaaaaaa tctgccaagt tttggagtgt caaccaggtg atttgttgac
     1621 ctataaaaat gactcgaata ctataccaga agaataaaat aaagtagaac aaacagtaaa
     1681 taaaaaataa tctgaatgat agatgtgtga gacttttaga agttatttac gtctattatt
     1741 cagatttttt atttcgattg aataccgttt tatctataaa taagatatgt actgactcat
     1801 acaaaggggg ccttttcttc tccatgtact atcacttttc ctgatacaac tggtttcctg
     1861 ttacgttgta gttggatctg cttttagtat aatactttat tcattccacc agactgatag
     1921 cagtttgagc agtctaaata atcatgtagt attacaggcc attctgtttc ataccttgaa
     1981 gtgcttaaaa cttcagctaa tggcccattg tgttaactct tactccattc ttttttagta
     2041 agctcccata tatactgtaa agccttctat atattatgtc atgttgcttt ttaacattta
     2101 ctatgtagtt ttgataaaat gatttgaata acagtgatgg acaattcttt tttattttta
     2161 acattatttt gataataatg attatttatt taaaaataca ttagataaca tttgttacga
     2221 cacgaaaaag cttcacaaac aaatatcgtt attttaaaat gagggcgaac tcacacaatt
     2281 aggaggaata gtatgccatt tattgtttac ttttttcttt cattactcgt gatctatatt
     2341 ccattaccaa catttttcct gctcgtcaat cgtctagcag agcaacatga tacgattgct
     2401 gctcttacga aactttgttt acttctattt gtttgtctag attactttac gagctattat
     2461 tggctaaaac agacaagagt aaaaaagaga tattatttct tccttgtctt tttcaatctc
     2521 tttgagtttc tgatccattg ctttttactt catcagtatc atacgatcca tttgtggatc
     2581 atctgggttt tcaaagtggt cgtattattt tgtatgttca cctttcctat ctcaagaaaa
     2641 tttagggagg cattgtttta ttaaagaagc ttctctgtat caataaagac acatttaagg
     2701 aaaaactttt aaaaatctaa ttgggaattt gcccgcataa ataagaggct gtgacaaaag
     2761 tcttgtcaca gcctcttatt ccgtataaac agtgttcaaa agcttgcatc ttcgataata
     2821 tgcccaacca aatgaaaaat atgagaagct atttttgttt ggctgttctt attacttgat
     2881 gaagaacagc tttttcacga cctcttttta aaagtctttt tacagttttc gagcaaagaa
     2941 tgatacgatt agtactaaaa tgatggcacc aatcaatgat ggcacgattg ccattcctgc
     3001 aatacttggt ccccaagaac ctaataatgc ttgaccaagt gctgatccaa ctaaacctgc
     3061 tacgacattt gcaataatcc ccatcgaaga acctttattc gtgattgctc ctgcaattgc
     3121 tccgataatt cctccgacga taagtgacca aataaatccc atgtttcgtt cctcctaatg
     3181 ttttacttcc ttattactga acccgtggtt ctgtattttc ttttactttt tgtgcgccgc
     3241 ttccagctgc tgattttact ttatctgttt gattcgaagc aaattcaccg gtttttttcg
     3301 ctgcgtcagt tactttgtct tgcaccgttt cttgatcttt ttcaaattct tcttttgttt
     3361 tgatgtccac tacgttagcg ttgacttcga tcacttctaa atgtgtcata ttttttactt
     3421 ctttgccgat tacttcttgc atttcttcaa agattttttc gatatctttg ccatattcca
     3481 caatgacatc catatcaact gctacttgtt ttttaccgac ttctgtttcg atcccagccg
     3541 ttacattatc tgtattcaca agttttcctg ccacattaga gaagaaacca ccgtttgcag
     3601 ataataaacc gtctacttgt tcaattgcga ttccgatgat tttttgcaca actttatctt
     3661 cataattcag ctcaccgcgc attgaatctt cctgttcaga atcatgcttt ccttggttat
     3721 ttgtatgatc agaaccaagt tttccttgtc cgtttgtgtg gtcagaacta agtttttctt
     3781 gtgctttcgc atggtcagaa ggatgttttt gctcttccac aggtttagaa ggatcgattg
     3841 catcatgttg ttctggaact ttagattctg gagttcctgg atgatgaggt gcatgctttt
     3901 ctgcatcatg tttcttctct tctactcctt gttgaacgtt agggactact ggatctggat
     3961 gtgcagatac tggtgctgtt ttgttctttt catcgttcac accatttgat tggtttggtg
     4021 tcgttgggtc tggatgtgca gacactggtt tttggttgtt cattttattg ttattatttt
     4081 tgttattgtt attcatgg
//
LOCUS       DACGLW010000139         4066 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.147, whole genome shotgun sequence.
ACCESSION   DACGLW010000139 DACGLW010000000
VERSION     DACGLW010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.147"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            85..1434
                     /locus_tag="IXT53_002816"
     CDS             85..1434
                     /locus_tag="IXT53_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ4906576.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(1562..2152)
                     /locus_tag="IXT53_002817"
     CDS             complement(1562..2152)
                     /locus_tag="IXT53_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HAQ4906577.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            2299..3651
                     /gene="brnQ"
                     /locus_tag="IXT53_002818"
     CDS             2299..3651
                     /gene="brnQ"
                     /locus_tag="IXT53_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HAQ4906578.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         1172 a          744 c          789 g         1361 t
ORIGIN      
        1 aaattttgga atcttagttg tgtttttcag aaaactctct taatatggaa gtaattaaac
       61 ttttttagtt aggatgattt aaaaatgaat gaagagcaag aactgcagcg tggactgaaa
      121 aatagacatg tccaactgat ttctattggc ggagcgattg gaacggggtt gtttttaggt
      181 tccggaaagt ctatccagct agctggccct tcgatattgc tcgcctatct tattactgga
      241 tgcatctgtt ttttcattat gcgtgcgtta ggcgaacttc tgttatcaaa caccaataac
      301 cattcttttc tggattttgt tgcagaatat cttggcaaaa aagcggcttt catcactggc
      361 tggacgtatt ggttctgctg ggtatcgatt gcaatggccg atttaacagc catcggaatg
      421 tatgtacgct actgggcgcc tggtgttcct cagtggcttc ctgaactgat tgcattaggt
      481 cttcttcttt gcttgaatat ggtggctgtc tcattgtttg gtgagttaga gttttggttt
      541 gcattgatca aagttgtagc gatcattgcc tttatcctcg taggagctta tatgatcctc
      601 acccactata cgacagatat tggcacagcc agtatcacga atctatggtc tcatggcgga
      661 ttctttccaa ctggtgcaaa aggatttatc ttagctttcc aaatggtaac ctttgctttt
      721 gctgggatag aattagttgg attagtcgct ggagaaacag aaaatcctga aaaagtgttg
      781 ccggaagcta tcaataatat tcctatcagg atcatcttat tttacttagg atctcttttt
      841 gtcattatgg caatctatcc atggaacagc ttgaatccag ataacagtcc atttgtagaa
      901 gtattttcgg aaattgggat cacgattgct gcttccttga tcaatttagt cgtattaagt
      961 gcagcagctt ctgcctgcaa cagtgcaata tatagtacag gacgtatgct tcgttcttta
     1021 gcgcaagaag gcagtgcccc taaaaaattc aaaaagttaa caacacatca tgtgccaggg
     1081 aatgctttga cattttctac tatcgtcatt ttcatttctg ttatcttgaa ttacgtgatg
     1141 cctagtgaag tattcacact agtaagcagt attgccacta cttgtttctt gtttatctgg
     1201 agtatgcttg tctacacgca catgaaatat cgtaaaagta tcctcggcaa aaaagctcat
     1261 tcatttaaaa tgccgttgta cccatttagt aattatctgg tatttcttta catggccttt
     1321 gtgtgtgtcg tcttgttcct aggaaaagat acgagaatcg ctttattact aacacctgtg
     1381 tggttcttgc ttctattact aatctatcat atgaaatatg aacgtaagaa gtaagaaagt
     1441 agttgtgaca gaagtggtca gcttcgagaa ataagaagga atttacgaaa attgcttctt
     1501 aaatttttgt gaaatttcgg cttatttctt ggagataaaa gcttctgtcc cagcctcttt
     1561 tctatttccc cggaaacaac cgctgataat aggcatctgc ttttttatag gaaaaaatcc
     1621 aaataccaga tacaaaaaaa cgtccgaata acccgatcaa cggtacaaaa aagacaccgg
     1681 caatcaagca taagttaatc atcatggaga taagacttcg acggtccttt gacaaaactc
     1741 tcacgacatg ggaatctggt ttatttgctt tcatgatttg cctcatcaag ataccaaaag
     1801 aaaaactcca catcgtgtat actaagacat aagccatctc aggccaaaaa gcttcagggt
     1861 tacgcccgac ccaactagta acagaaggac ataaagtcag ccaaaagagc aaaaagatat
     1921 tcgaccagac cactcggtaa ttaattcgtt ctgccatctg aaatatacga tgatggttca
     1981 cccagatcac cgcaacaaat ataaagctga tggtataagc taataatgta tttcggatga
     2041 cccataaaga atgaaagcta gatgtattag ggatcttgat ctctaataca agaactctca
     2101 aaacaatggc taccactcca tcagtaaatg cttctaatct cgactttgtc acgatgattc
     2161 ctcctcagat gattcagcgc ttttatttta tcacttacgt tattaaataa ccatcgtttt
     2221 atatagggaa ttttctgaaa attgttgcat atttttcgaa attttgttac ttttaaaaaa
     2281 accgtaaagg agttatgtat gacaaaaaaa ttatcattta aagactatct ttttataggc
     2341 tccatgcttt tcggtctatt ttttggtgct ggaaatctta tctttcccgt tcatttgggt
     2401 caagaagcag gcgctgctac cttttgggcg aatctaggtt tcttggttac cggtatcggt
     2461 cttcccttct taggggtgat tgccattggt gtatcgaaaa gctcaggtgt atatgaatta
     2521 gcaaaacgga taaatcaaac atatgcgatg gtttttacca tcttgcttta cttagttatc
     2581 ggaccgtttt ttgctttacc tcgccttgca accacatctt tcgagattgg tttagcacca
     2641 tttatcgatc ctagcagaca gacgctatat ctagcaattt tcagtattgt attttttgtg
     2701 ctggcttggt ggttttcaag aaaaccgaca aagatattag attatgttgg aaagttcctg
     2761 aatcctgcat ttttggtttt attaggtgct ttgcttctcc ttgctttttt gaatccacta
     2821 ggttctgtaa atcaagcgcc tattcagcca aattatcaag agcatgcttt tttcacaggg
     2881 tttactcaag gatataacac attagatgcg cttgctgctt tagcatttgg gatcatcatt
     2941 gtcacaacaa tccaaaacat gggtgtaaca aaacctgctg aaatcgctaa agacacgatc
     3001 aaatctggtg cgatcagcat tatattgatg ggaatcattt atactttgct cgcttattta
     3061 ggtgctatga gcttaggtag ctttgctcta agtgaaaatg gcgggatcac gctggctcag
     3121 attgctgatc attatttagg tacttatggc agtatcttat tagcatttat cgttatatta
     3181 gcttgcttga agaccggtat tggtttgatt acagcttttg cagaaacatt cacagatttg
     3241 tttccgcaaa gaaattacgc attctttgtt gcacttgcta gtatccttcc ttgtttagcg
     3301 gcaaacgtag gcttgacgaa tatcattcaa ttttcgttgc ctgttttgat gttcatctat
     3361 ccattagcaa tgacactgat tttactagtg ttagttggtc ctttgtttaa gcagcgtacg
     3421 gctgtttatc gaatgactac ttattttaca ttgattgctt cgatttttga tgggttgaac
     3481 gcttgcccag aaactatcaa acaaacacct attgttcaaa atcttttata tgttgcggaa
     3541 agctacttgc cgtttttcaa attggggatg gggtggatcg taccagcagt tatcggtttt
     3601 gttatcggac tgatttggag tttcgctaag aaagaagaag tagcagattg ataaaagagg
     3661 ttgtgaaaga gacgttcagc cttgagcaat aaaaagtaat tttgaaaaat ggcttctcac
     3721 atttttgcat aaatttgact taataccgag aaactgactt ttgaacaccg tttattgaga
     3781 ggtcgtgaaa aagccgctta gtttcaagta ttaagaaata ttttgtgaaa atggcttctc
     3841 acatttttgc ataaatttga cttaataccg agaaactggc ttttgaacac cgtttattga
     3901 gcgtctataa actgacctga gtattagaga aacaaaacgg caaagtagct ccatatactt
     3961 tgccgttttg ttttgaacta ataccgaaga gaagctgatc aaacaacatt tcttttataa
     4021 tatagatttt agacatatat atgatataaa aatagttttc gcaaaa
//
LOCUS       DACGLW010000140         3866 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.148, whole genome shotgun sequence.
ACCESSION   DACGLW010000140 DACGLW010000000
VERSION     DACGLW010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3866
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.148"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            1..666
                     /locus_tag="IXT53_002819"
     CDS             1..666
                     /locus_tag="IXT53_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906579.1"
                     /translation="MAYYLLSEPKQIAASNIFTAVMTLMCVMCFSIFSYLLVQFIRQN
                     LTMSKLFKSLLDLLILGSMLTTLTDRFEQSEKTVEGIKNFLTTIDQFINGYMYLFEVG
                     FILTLFGCVFIKLIKRSIEKEMVIDLIPTLSGYEYYVDFGKYEELIHTDIVHTSIHEV
                     TVKGIRSTLKDGQETKTIDQRKEVYNFYKVEASLRYGIGKLRLDGSNIQQNKPYVLGK
                     VVI"
     gene            710..979
                     /locus_tag="IXT53_002820"
     CDS             710..979
                     /locus_tag="IXT53_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906580.1"
                     /translation="MILLYRIVLIVGTELKILKKEQLFKVKEHHNQLLCIKTGTMNIQ
                     YRTEYLELTNQTDFLSIREQHKQEKLMATYSQLIDEWEQFKESKT"
     gene            1211..1507
                     /locus_tag="IXT53_002821"
     CDS             1211..1507
                     /locus_tag="IXT53_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906581.1"
                     /translation="MNVKIRDLDPKFISEIDRRCVELSNRTGNKWSRNDYLKLLVEND
                     FDRPLMEYKQEKFDQLLEKFSEIQSYNTKILEEYVSQNNRIIEILIEQNRGSEL"
     gene            1509..1931
                     /locus_tag="IXT53_002822"
     CDS             1509..1931
                     /locus_tag="IXT53_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906582.1"
                     /translation="MAKVIDKHIYFHQTHIDVLDETISTHAEVKNYSEAVRFLCMNYD
                     PEVEKKVNSNQAKLNGMSKEISILTEMVSEFADLHLKDTGILIGKNSTVYQEAKERVE
                     GKIKSNQTKKYSPQLKKPVNESVHARVDPFDPTKILKR"
     gene            1950..3542
                     /locus_tag="IXT53_002823"
     CDS             1950..3542
                     /locus_tag="IXT53_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906583.1"
                     /translation="MTQSPSVILTCQFTLPNKKSFSTYIDYITREQALEEKERRTPEE
                     EQELQRIKNALEEFYIPEGETFSESKTGKEFSETSNEAQSLMEHGINFDKLEEQDFTN
                     YLSYMTRHYALEQKKELTQSEQKEYQRLNQAMKKYQTEPHKKASGNLPGVFSIASDEV
                     KGSDLKEIKQIFQRGQQKGSIIYQDVVSHDNSYLEKIGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKENTRKPYTEVENGVSYIVPKG
                     KRRQATLDAMKTNYVHELERFSYEKDRMIGMEKRNLLTRKSDLRNTLTKVVKEPARYD
                     RHAMNLLNEVYKTLPDKRSEWNYGDEKRTKLSPYTREKLDELTKYVLTKEPEYQEYIQ
                     ISKALKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKRQDSQIKPLIESVD
                     QMQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEE
                     KYHASKAYEEQQRRIQMEQERQ"
     gene            3563..>3866
                     /locus_tag="IXT53_002824"
     CDS             3563..>3866
                     /locus_tag="IXT53_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906584.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLRNEQF
                     DPNQFLVVEYPATGVYCCNHVNGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFPCKS"
BASE COUNT         1518 a          554 c          758 g         1036 t
ORIGIN      
        1 gtggcctatt acttgttaag tgagccaaaa cagatcgcgg cgtcaaatat tttcacggca
       61 gtaatgacat taatgtgtgt catgtgcttt tctattttta gttacttact cgtccaattt
      121 atcaggcaaa atttaacaat gagtaaactt ttcaaaagtt tattggactt acttatctta
      181 ggcagtatgt taacgactct aacagatcgg ttcgaacaat ctgaaaaaac ggtagagggg
      241 ataaagaact tcttaacgac gattgatcaa tttataaatg gatatatgta tttgtttgaa
      301 gtaggcttta ttctcacttt gtttggttgt gtatttataa aactcattaa aagatcaatc
      361 gaaaaagaaa tggttattga tctaattccg acgctgagcg gctatgagta ttacgttgat
      421 tttggaaagt atgaggagtt gatccatacg gatatcgttc acacgagtat ccatgaagta
      481 accgttaagg gcatcagatc tactttaaaa gatggacagg agacgaaaac aatcgaccaa
      541 agaaaagagg tctacaattt ttataaagtt gaagcaagtc tacgatatgg cattgggaaa
      601 ttgaggttag atgggagtaa tattcaacaa aacaaaccat atgttttagg aaaagtggtg
      661 atttagaact tcctatgaaa gatgaataaa taaggagaat cttttgaaaa tgattcttct
      721 ttacagaatt gtgcttatag ttggcacgga gttaaaaatc ttaaagaagg agcagttatt
      781 caaagtgaaa gaacatcata atcagttact atgtattaaa acgggtacga tgaacattca
      841 ataccgaaca gaatatttag aattaacgaa tcaaactgat tttttgtcga ttcgagagca
      901 acataagcaa gaaaaattaa tggctactta tagccaattg attgatgagt gggaacaatt
      961 caaggaatct aaaacataaa attccattta aacaccttat tttttaaaga gcataaggaa
     1021 acgtgacccc acttttaaaa aaagtggttg gataggctgt gcctatcacg tttctgaaaa
     1081 aaataaatga ccccaaaata ccaatatata ggatagtaca aggatagcaa aggatagtaa
     1141 aaggatagag gtaggatagc gaaaaggata ggatcctttt cactatccta tattatattg
     1201 gaggatattt atgaatgtta aaattagaga tttagatcca aaattcattt ctgaaattga
     1261 tcgaagatgc gttgaactaa gtaatcgtac aggaaataaa tggagtcgta atgattattt
     1321 aaagctactc gttgaaaatg attttgatcg tcctttgatg gaatacaaac aagaaaagtt
     1381 tgaccagtta ttagaaaagt tttcagaaat tcaaagttat aacacaaaga tattagaaga
     1441 atatgttagc caaaataatc gtattataga aatcttgatt gagcaaaata gaggaagtga
     1501 actttgatat ggcaaaggtt atagacaaac acatttactt tcaccaaaca catatcgatg
     1561 tattggatga aactatttct actcatgcag aggttaaaaa ctatagtgaa gctgttcgct
     1621 ttttatgtat gaactatgat ccagaagtgg agaaaaaagt aaattccaat caagcaaagt
     1681 tgaatggaat gagtaaagaa atttctattt taaccgaaat ggtttcagaa tttgcggatc
     1741 ttcatttgaa agatacagga atattaattg gtaaaaattc aactgtttat caagaagcaa
     1801 aagaacgagt ggaaggaaaa attaaatcca atcaaacaaa aaaatattcc cctcagttga
     1861 agaaacctgt gaatgaatct gttcatgcca gagtagatcc atttgatcca acgaagattc
     1921 taaaaagata aaaatgaagg aggataataa tgactcaatc gccttcagtt attctcactt
     1981 gtcaatttac tttgccgaat aaaaagtcat tttctactta tattgactat attacaaggg
     2041 aacaggctct tgaagaaaag gaacgacgta cgcccgaaga agaacaggaa cttcaacgaa
     2101 tcaagaatgc tttagaggag ttttatattc ctgaaggaga aactttttcg gagagtaaga
     2161 cgggtaaaga attcagtgaa acgtcgaatg aagctcaatc gttgatggaa catggaatca
     2221 attttgacaa gttagaggaa caagatttta ccaattatct ttcttatatg acacgtcact
     2281 atgcgttaga acaaaagaaa gaactgacgc aaagtgaaca aaaagaatac caacgcttga
     2341 accaagcaat gaaaaaatat caaacagaac cccataaaaa agcttcagga aatcttcctg
     2401 gagttttttc tatagcttca gacgaagtga aaggaagtga tctgaaggag attaagcaaa
     2461 tcttccaaag aggacaacaa aaaggctcaa tcatttatca agatgttgtt tctcatgata
     2521 attcgtatct tgaaaaaata ggattgtacg atcccaaaac agatacttta gatgaagaag
     2581 ggttaaaagc agctggaaga aaaatgatgg agacattatt cgagaaagaa caattgaatg
     2641 aaacaggtta ctggatggca acgattcacc gtaacactaa acatatccat attcattttg
     2701 cagttgttga aaaagaaaat acgcgtaaac cttacacgga ggttgaaaat ggtgtgagtt
     2761 atatcgtacc gaaaggtaag agaagacaag caacattaga cgcaatgaag accaattatg
     2821 tacatgagtt agaacgtttt tcttatgaaa aggatcgaat gattggtatg gagaaacgaa
     2881 atttattgac gcggaaaagt gatttgcgta acaccttaac aaaagtggta aaagagccag
     2941 cgcgttacga tcgacacgcg atgaacttat taaatgaagt atataaaacc ctacctgaca
     3001 aacgttcgga atggaattac ggggatgaaa aacgaaccaa actttcacca tacacaagag
     3061 agaaactcga tgagttaacc aaatatgttt taacaaaaga acctgaatat caagaatata
     3121 tacagatttc aaaagcattg aaagaagaag caaaaagtct ttatggggaa agcaagagag
     3181 aatcaaagga tgcagagaaa aatgctctct ttgatttaaa aaagcgcaca ggaaatgcca
     3241 tcctaacaga gctgaagaga caggatagtc aaataaaacc attaatagaa agtgttgatc
     3301 agatgcaatc tatttacaat gaacgatcct ttcgaacgct taaacaattg ccaacgaatg
     3361 aatacaaata tagttacgaa gaattcatga agcgcaggaa acaattccaa caaagagttg
     3421 tcacgcagcg gagtttgcgt caattaaatc gaacgattaa tgcccatgaa gaaaaatacc
     3481 acgcaagtaa agcatacgaa gagcaacaac gaagaattca aatggaacaa gaaagacagt
     3541 aaagacagta ggaggaatag caatgaaaca atatttagat caatggaaag ttatagaagg
     3601 atcattaaga gaagaacgaa tcgaacaact tcctgattgt ttagaaaaag agcatttgtt
     3661 ccagataaga gaaatgttga gaaacgaaca gtttgatcca aatcaatttt tagtcgttga
     3721 gtacccagct actggtgtgt attgttgtaa tcatgtgaat ggtgaaaaat attttatcat
     3781 tcaagaatat gagggcaagt tagcacctta ttatactact tgggaaatga atgaagaggg
     3841 aatcaataac tttccatgta aaagca
//
LOCUS       DACGLW010000141         3809 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.149, whole genome shotgun sequence.
ACCESSION   DACGLW010000141 DACGLW010000000
VERSION     DACGLW010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.149"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            complement(12..323)
                     /locus_tag="IXT53_002825"
     CDS             complement(12..323)
                     /locus_tag="IXT53_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906585.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            504..1979
                     /locus_tag="IXT53_002826"
     CDS             504..1979
                     /locus_tag="IXT53_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HAQ4906586.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            2026..2610
                     /locus_tag="IXT53_002827"
     CDS             2026..2610
                     /locus_tag="IXT53_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HAQ4906587.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            2612..3031
                     /locus_tag="IXT53_002828"
     CDS             2612..3031
                     /locus_tag="IXT53_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HAQ4906588.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(3312..3596)
                     /locus_tag="IXT53_002829"
     CDS             complement(3312..3596)
                     /locus_tag="IXT53_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HAQ4906589.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
BASE COUNT         1204 a          778 c          710 g         1117 t
ORIGIN      
        1 ttaattattt tttataattc tggtatactt ttctttcctt tgtaccacaa ctcttctggc
       61 gtatacctgt cttttccatt tatgaatact gttccatctg cttcaatcgt tacttcagtc
      121 ccaatccaca tattacgaga aagtgcgaga agattttttc tttttcctct tccttttttt
      181 ccaaattgca aggcatcggt tatctcttct gtccaatgag atttcacgta ttttacttct
      241 ccgttttcaa atgtaacttt caaaatattc gctatatttt ccacttttac tgctttagga
      301 aatgactcct ccattttttt cactttcctt cctccaacac taagtatgcg ctttctttta
      361 gtgataagta tatcatagtt tattctatta tcaaaaaaac gcatccattc cattgtagaa
      421 tactttaccg aacaagttga attgttttcc tctttttcac cttttactat agataggtaa
      481 agacaactaa ggaggaatct ctcatgacag atcaaccgta tgatttaaga aaagtcatcg
      541 aagaactaaa aacacttcca ggtcaatatc acgaaacaga tattgaaatc gatcctgaag
      601 cagaattatc tggtgtttac cgttatatcg gtgctggcgg tacagtaaaa cgtccgactc
      661 aagaaggacc agctatgact ttcaacaata tcaaaggttt ccccggcaca cgtgtgaaca
      721 tcggaacaat ggctagtcgt aaacgtgtag gtgaaatttt gcaccatgat tatcgaacac
      781 ttggtcgttt cttgaaagac gctgtagaaa atccaatcaa acctgtgaaa gtagacaaag
      841 cagacgctcc agcacaagaa gtcgttcatc tagctaccga tgcagacttc gatattcgca
      901 acattttgcc tgcacctaca aatactcctg aagatgctgg tccatacatc acaatgggtg
      961 tagtcttagg atcagatcca gaaaaaacaa tgagcgatgt aacgatccat cgtatggttc
     1021 tagaagataa agatacgatc ggtatgtaca tcatgcctgg cggacgccac attggtcact
     1081 tccaaaaaca attcgaagcc ttgaataaac caatgcctat cactatcaat attggtttag
     1141 atccagcaat tgccattggc acgactttcg aaccacctac cactccactt ggctacaatg
     1201 aactatggtg tgcaggagct ttgcgtaacg aacctattca aatcgttgat ggtatagcag
     1261 tcgatgaagt gggtattgct cgtgctgaat tcatcattga agcagaaatc atgccaaatg
     1321 aaacaatgca agaagatatc aacacgaact caggaaaagc aatgccagaa tttcctggct
     1381 atactgggga tgctaatcca gctgtacaag tagtgaaagt caaagcagtt actcaccgaa
     1441 aagacaatcc aatcatgcaa acaacgattg gtcccagtga agaacacgta tctatggccg
     1501 gtattcctac agaagcaagt attcttgcct tggtcgataa agctattcca ggaaaagtag
     1561 taaatgtcta caatcctcct gcaggtggtg gtaaattgat gaccatcatg caaatccgta
     1621 aagaaaacga agcggatgaa ggtattcagc gtcaagcagc tattcttgct ttatcagcct
     1681 tcaaagaatt gaagacagtc attttagtcg atgaagatgt tgatattttc gacatgaacg
     1741 atgtagtttg gactttcaac actcgtttcc aaggagataa agatatcatc gtgatcccag
     1801 gaatgaggaa ccatccattg gacccatcag aattaccaga atataatcct ggctctattc
     1861 gtttccgtgg gataagtgca aaaactatct ttgacggtac agttcctttc gatatgaaag
     1921 aacattttgt acgtgcaaaa ttccaagaag tagaagattg ggaaaaatat ttgaaataat
     1981 gaatgatctg caactgtttt gacatcataa aaaggagtgc ggaaaatgga acaattggca
     2041 caacggaaga aaaaaatcgt gattggtgta agtggcgctt caggcaccat ttatgccatc
     2101 aacctagtga agaaactaaa agaatatccg atgatagaaa cacacggaat catcagtcca
     2161 tgggcaaaac agaacttaaa gctggaatcc gacttatcac taactgaatt cgaaagcctg
     2221 tttgattacc attattcaaa caaagattta ggtgctgctg tcgccagcgg atcatttttg
     2281 accgatggaa tgatcatcgt accggcaagt atgaaaacag tcgcaggtat ctctgttggt
     2341 ttaggcgaca acctgattgc acgtgctgct gatgtaacaa tgaaggagca acgaaagtta
     2401 gtcattgttc ctcgtgaaac gccattaaat gccattcact tggaaaactt gactaaatta
     2461 gcacgactag gtgtgcaaat cataccacca attcctgctt tttataatca gcctcaaacg
     2521 atccaagatc ttgttaatca ccatacaatg aaacttttgg atcaatttga aattgtaaat
     2581 gagcattcaa aacgttggca gggtgattaa tatggaaaaa gaaacgatag ttgaagtaac
     2641 caaactaggt ttcacgatcc aagctgctgt taaagtaatc ggtaaagact tattgatcac
     2701 tttaacaggc ggagatacac cacatatcgg aacagtcact acattttctc gtgatacaga
     2761 gatgcaaact gtacgtttcc ctagccacga tggtcggttc cataaagacg atgtcttagc
     2821 cgaaaaaata gctgtgatca tcaaaactgc cgttccggga agttgtacga tcacttccgg
     2881 tatccatgtt gaccatatct ccaatgcaca aatcaacgcc tcttttccga tgtcagaaga
     2941 gttaggttac aagattttag attggttgga aaaaatagat ttccatatca aagaaccagt
     3001 ttattataaa gatggtgaaa aaccattatg aaagaagagg ttgtgaaatc agcgttatga
     3061 cctgagtatg agagaaaaaa atggaaaaat agcttctcat attttgccgt ttttgagcta
     3121 ataccgaggg acagctgatt gaacgccgtt tatcaagagg ttgtgataaa gccgcttcgt
     3181 ttcgagtagt aagaaaaatt ttgtaaacat gggtttccac atttttgcat aaatttgact
     3241 tactaccgag aaactggctt ttgaacaccg tttatcaaga ggttgtgaca cgtcttttgt
     3301 cacaacctct tttatttttg cttcactgta actgctacaa tatcaccgat ttcttttccg
     3361 atgtttttac gaatatcttt tcgtagtcca aggatgtagc agactgaacc atctggattt
     3421 ttcacgccca tattcacgat actgccttca tacggcacat tgtcaaaagt tgcttggact
     3481 ttaactcttc cagcaccgaa ttcttttctt atatcatacg gaaaaataat atatgccccg
     3541 cccttgccga cttcggaagc atagattttt gattcaaaag aataaatttc cctcatttta
     3601 tttctcctat tttttacatc tagtatagca tagcaaaaaa aagaaggaac tgtgacaaaa
     3661 gtcttgtcgc agttcctttt ttcgaataaa cggtgttctc ctgccaactc ctcggtcaca
     3721 agtcacaata gtcaacaaac atgagaagct gttttttgaa attgctcctt gtgactcaga
     3781 gttaaacggc aggctcacaa cctcttcct
//
LOCUS       DACGLW010000142         3666 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.150, whole genome shotgun sequence.
ACCESSION   DACGLW010000142 DACGLW010000000
VERSION     DACGLW010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3666)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3666)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3666
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.150"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            79..234
                     /locus_tag="IXT53_002830"
                     /pseudo
     CDS             79..234
                     /locus_tag="IXT53_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
     gene            253..1530
                     /locus_tag="IXT53_002831"
     CDS             253..1530
                     /locus_tag="IXT53_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ4906590.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            1540..2196
                     /locus_tag="IXT53_002832"
     CDS             1540..2196
                     /locus_tag="IXT53_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HAQ4906591.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            2196..3572
                     /locus_tag="IXT53_002833"
     CDS             2196..3572
                     /locus_tag="IXT53_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HAQ4906592.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGVVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
BASE COUNT         1481 a          380 c          626 g         1179 t
ORIGIN      
        1 catgaaaaat cctccagtgt gttttttatt attctacaca ccttattttt tctgtctagt
       61 ctagtgtagc cgattcagga taaggataag tgtattaatt gtggaaaatg taaaaaagtt
      121 tgccaaatga ataaagattc agttgagaat tgcaatcatc tggagtgtat cagatgtgga
      181 cgatgtaaaa atgcttgtcc agtggatgca attagttatg gagtaagaaa atagagagga
      241 gtagttagga aagtgataaa aaatgctata gcatatatta caagaaagaa aaatagaact
      301 ttaataattt ttatcatatt aactatagtt ctttcttgct tgtattcttg tttaacaata
      361 atgaaatcaa gcaatgaaat agaaaaggct ttatatgaaa gttctaattc ttcaatatca
      421 ataacaaaaa aagatggtaa atattttaat gttaatcaat ttaaagatat tgaaaaatta
      481 aaagaaattg aagaaaaaat aattcaatat gatggattag caaaactaaa agatgctaaa
      541 gtagttagcg gagaacaaag aataaataga gaagatttat ctgacgaatt taagaatgtt
      601 gtttcactcg aagctacaaa taatactaaa agaaatattt tatttagtag tggagtattt
      661 acaattaaag aaggtaaaaa tatagaagaa aatgataaga attcaattat tgttcatgaa
      721 gaatttgcta aacaaaacaa tctaaaatta ggtgatgaag ttgatcttga attactagat
      781 attgaaaaaa gtggaaaaat aaaaagtcat aaatttaaaa ttatagggat cttttctggt
      841 aaaaaacagg aaacatatac aggattatct tctgatttta gtgaaaatat ggtgtttgta
      901 gattattcaa caagccaaga gatattaaat aattcagaaa ataatgaaat tgcaaataaa
      961 attttaatgt attctggtag tgcagaatct acagacttag ccttaaacaa attgaaagag
     1021 cttaaaattg atgagtcaaa gtattttgtt gaaaaagatt ctaatgcgtt tgaagagtct
     1081 ttagagtcag tgagtggaat aaaatatata ataaaagtaa tgacttattc tattatgtta
     1141 ggtgggatgg ttgttctttc attaatcttg attctatggt taagggaaag aatttatgaa
     1201 ataggtatat tcttatctat tggaacatct aagatacaaa ttataatgca atttatattc
     1261 gagttaatat tcatatcgat accaagtata atatcttcct tatttttagg aaatgtatta
     1321 ctaaaagtaa ttgtagatgg attcattaac tcagaggatt caatgatttc cggtggaagt
     1381 ttaataaata atagtagttt tatgtcaaat ataacaacac ttggacaaag ttatttaata
     1441 ttaataagta ttattgtttt atcagttgta tttgcttctt cattaatgtt aatcaagaag
     1501 cctaaagaaa tcttatcaaa aataagttag gagaaaataa tggatatatt agaaataaag
     1561 aatgtagcat atagttatgc aaattctaaa gaaaaagttt tgtcaggagt aaatcaaaaa
     1621 tttgaacttg ggaagtttta tgcgatagta ggaaagtcag gaacaggaaa atctacactt
     1681 ctttccttac ttgctggact tgataaacct caaacaggaa aaatattgtt taagaatgaa
     1741 gatatacaaa ataaaggata tagtaatcat agaaaaaata atatatcttt agtatttcaa
     1801 aactataatt taatagatta tttatcacca attgaaaata ttagattagt aaataaatca
     1861 gcagatgaaa gtatcttgtt tgaactaggt ttagataaaa aacaaataaa aagaaatgtt
     1921 atgaaattat ctggtggaca gcaacaaaga gtagctattg ctagagcatt ggtatcagat
     1981 gctccaataa tactagctga tgagcctact ggtaatctag acagtgttac tgctggagaa
     2041 ataattaata tattaaagac attagctaaa gatagaaata aatgtgtaat agttgtaaca
     2101 catagtaagg aagtagcaga ttctgcggat atcattttag aactaagtgg taaaaagttg
     2161 aaaaaagtaa ataaaatgaa tttggaggtt gaataatgat aaaaaatgca tttgcttatg
     2221 taactagaaa aagtttaaaa tcattaatta tcatattagt tattttatct atgtcaactt
     2281 taagtctcat tagtttatct attaaagatg ctacggacag agcttcaaaa gaaacatttg
     2341 ctaatataac aaatagtttt tctatggaga taaatagaca agtaaatcca ggaacaccta
     2401 gaggtggggg aaatgtaaaa ggtgaagata ttaaaaagat atctcaaaca gatagtatag
     2461 actcctatgt aaaaagaata aacagtgttg cagatttagt tgattatgat attattgaaa
     2521 ctcaagagac tcttgcgaat caatcacctg aaagagcaaa gaattttaaa agaacagtta
     2581 tgttaactgg ggttaacgac tcaacaaaag aaacaaaatt tgtatcagaa gcatataaat
     2641 tagtagaagg aaagcattta gaaaatgaag ataaaaataa aatcttaatg cataaagatt
     2701 tagctgaaaa aaataacctt aaagttggag ataagataaa aataaaatct aatttatttg
     2761 atgctgataa tgagaaaggt gcagatgaaa cagtagaagt agaaattaaa ggtctattcg
     2821 atgggcataa tagcggtgta gtaagtgcag cacaagaact atacgaaaat acattaatca
     2881 ctgatattca tacagctgct aaagtttatg gtaataccga agatacagct gtataccaag
     2941 atgcaacatt ctttgtaaaa ggtgataaga atcttgatag tgtaataaaa gatcttggga
     3001 aattagatat aaattggaga gaatataatt tgattaaaag ttcatcaaat taccctgcat
     3061 tacaacaatc tatatcaggt atttactcaa tttcaaataa attatttttt ggctcattaa
     3121 tatttgcagg agttgtagtt tcattattat tattcttatg gatgaatgct agaaagaaag
     3181 aaatagcagt attactatca ttaggtatat caaaattaga aatttttggt caattcctaa
     3241 ttgagatggt atttatatca atccccgcat tcgttggttc ctatttctta gctcaatata
     3301 cagctgacaa gcttgggaat aatatattga ataaggttac tggtgatata gctaaacaaa
     3361 tagctaaaca atctgcatct agccaattag gtggtggggc agaagctgaa ggatttaaca
     3421 agacattatc aagtttagat atcaatgtat tacctaaatt catgatttat gtagttatat
     3481 ttatgagttt agtacttctt gtatcattga ttatttcttc attcaatata ttaagaagga
     3541 atccaaaaga attattaatt gacaataatt aaaattttcg gtgcttttta gcaccaaaaa
     3601 tgagagtgta atataaaccg tgtaagtaac catcaatcct aggcgggtgg ttgcttcacg
     3661 gttttt
//
LOCUS       DACGLW010000143         3641 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.151, whole genome shotgun sequence.
ACCESSION   DACGLW010000143 DACGLW010000000
VERSION     DACGLW010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3641
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.151"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            <1..300
                     /locus_tag="IXT53_002834"
     CDS             <1..300
                     /locus_tag="IXT53_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906593.1"
                     /translation="QKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQV
                     ILKGKSYGKEIQTALPSEEFALGNSFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            361..1830
                     /locus_tag="IXT53_002835"
     CDS             361..1830
                     /locus_tag="IXT53_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3991 domain-containing protein"
                     /protein_id="HAQ4906594.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVGDDRLDHAVAFLWKDPQTGETVGASYQGTIVDFNRFGKRGTYKHIDKNPTPNHGFN
                     LKIGDPKHLKFFESSIDLLSYAALNREKLQDAWLVSMDGLKHHVISHYVEESISELRR
                     KQTFPQSIEICVDNDRAGHIFYEKEQMKGIVDPFTNKKIRCERGIPNDWQVPKEYKAT
                     YEAVAKEMSVEPEAIMAIHKTETNLQLTNQLVSAHDVQSTFGKMLAKGEPVETIDLKE
                     ACTTVAKELKVCERADGTYNFDRFYSRKANIKDVNAGILLSYKAEQYYKGYKKHEHEF
                     VPEVKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            1841..>3641
                     /gene="topB"
                     /locus_tag="IXT53_002836"
     CDS             1841..>3641
                     /gene="topB"
                     /locus_tag="IXT53_002836"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HAQ4906595.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFTGETVIT
                     YGFGHLVELDSPDMYDEKWKQWALEHLPIFPNQYHYHVPKDKKKQFSVVKRQLQSADT
                     IVIATDSDREGELIAWSIIQQAGANQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEVQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEAYLKEKGVQLGKQPGTILQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIEKVPLDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVS"
BASE COUNT         1341 a          625 c          795 g          880 t
ORIGIN      
        1 caaaagaata ttgttcctgt tggttcactt tggatcgaca agaaagacgg gaaagtaatg
       61 gaagaagata cgggtcgaac gttacatttg aaggatgcgg cgtttattga agatccacag
      121 cataatcaag taattttaaa aggaaaaagt tatggaaaag agatccaaac ggcgttacct
      181 tcagaagaat ttgcgctagg aaattcattc cgattttcac ttgagacagt gtcagaagtg
      241 gcaaaacgaa aaaatgaaat ctattatgaa caacaattgt cacaaaaaga acgtgtataa
      301 aacaagtaaa aaagactatc tgaaaaacgg atagtctttt ttcttttggg aggttttaaa
      361 atgaccgaga aaaaaatgag tcttattgat cgatgtaaac agatcgatat agtggatttt
      421 gctcgaaaca atggaatggc tgtagtcaat aaaggacgag actatcgctt agaagaccat
      481 gattcatttg tttttgatcg aagaaaacaa cgtttttatt ggaacagtca aaatattagt
      541 ggcgacatta ttgaattagc taaacttttt ttcatagaca aagagatcca agaccctaag
      601 caacaattta aagcagccac tgattttatt ctaaagaatg aagacaaaac agaacgagtg
      661 gaaaatcttc attttgagac agaaaagtac aaagatcacc ccgttgatta tcaaccactc
      721 acagaaaaag gaaggaatta tctgaaggaa gaacgaaaac taccagagtg gctgattgat
      781 tatgcagaaa aagaagggtt gattgctgaa ctaaagccga aacatgaacg gcaaaatttt
      841 ttagttggag acgaccgttt agatcatgcg gttgcttttt tatggaaaga tccacagaca
      901 ggagagacag taggggcaag ttatcaagga acgatcgtcg attttaatcg tttcggtaaa
      961 cggggaacgt ataaacacat tgacaagaat cctacgccaa atcacggatt taatttaaaa
     1021 ataggcgatc cgaagcattt aaaattcttt gaaagtagta ttgatctgtt aagttacgct
     1081 gcattgaatc gggagaaact acaagatgcc tggttagttt cgatggacgg gttaaaacat
     1141 catgtaatca gtcattacgt tgaagagtcg atttctgaat taagacgaaa acaaacattt
     1201 cctcaatcga ttgaaatatg tgtggacaat gatcgtgcag gacatatttt ttatgaaaaa
     1261 gaacagatga agggaatcgt tgatcctttt accaataaga aaatccgttg tgagcgagga
     1321 attccaaatg attggcaagt acctaaagaa tacaaagcta cttatgaagc agtcgcaaaa
     1381 gaaatgagtg ttgaacctga agcaattatg gccatacaca aaactgaaac taatttacag
     1441 ctgacaaatc aattggtgtc tgctcatgat gttcaatcta cgtttggaaa gatgttggcg
     1501 aagggcgaac cagtagaaac aattgatttg aaagaagcat gtaccacggt cgcgaaggaa
     1561 ctgaaagtat gtgaacgcgc agatggaaca tacaactttg atcgttttta tagtagaaaa
     1621 gccaacataa aagacgttaa tgcaggaatc cttctttcct ataaagcaga acaatactac
     1681 aaaggctata aaaagcatga acatgaattt gtacctgaag tgaaaaagga ttggaatgat
     1741 caattgaagc atgagatcca gcaacaagaa atcagaaagc aaaaacgtgc gatgttgttt
     1801 caacaaggca gacaacaaga aagggagtga agtcgtcact atgaaaaccg tcattttagc
     1861 agaaaaaccg tcacaagcga aggcttatgc cgatagcttt tctaaagcta cccgaaaaga
     1921 tgggtatttt gagatccaag atcgattgtt tacaggagaa actgtgatca cgtatggttt
     1981 tggtcattta gtcgagttag attcgcccga tatgtacgat gaaaaatgga aacaatgggc
     2041 attagaacat ttgcctattt tccccaatca ataccactat catgttccta aagataagaa
     2101 aaagcagttt agtgtcgtca aacgacagct gcagtcagct gatacgattg tgatcgcgac
     2161 tgatagcgat cgtgaagggg agctgattgc ctggtcaatc attcaacaag caggcgccaa
     2221 tcaaggaaaa acctttaaaa gactttggat caactcctta gaaaaagaag ccatttatca
     2281 gggattccaa cagttacgag acgcagaaga gacctatcca aaatttgaag aagtacaagc
     2341 acgtcagatc gccgattggt taattgggat gaacggtagc ccactgtata gtttactctt
     2401 gcagcaaaaa ggcataccag gaagtttttc tttaggtaga gtccaaaccc caacactcta
     2461 tatgatttat caattgcagg aaaagattag aaacttccaa aaagaaccat attttgaagg
     2521 aaaagcccaa gtaatcgctc aaaatggcgc atttgatgcg aagcttgatc caaatgaaac
     2581 ccaagcgaca caagaagcat ttgaagccta tttaaaagaa aaaggtgttc aattaggaaa
     2641 acagccaggt acgatccttc aagtcgaaac agagaaaaaa agtgcggcta gcccacgtct
     2701 tttttcttta tcgagccttc agtctaaaat gaatcaactc atgaaagcta gtgccaaaga
     2761 tactttggaa gccatgcaag ggttgtatga aggaaaatat ctaagctatc cgcgaacgga
     2821 taccccttat attacggaag gggaatatgc gtatctgttg gatcacttag acgaatacaa
     2881 gcatttttta aaagccgaag cgattccgac tcctattcat acaccgaata gtcggtatgt
     2941 gaataataag aaagtccagg aacattacgc gatcattcct acaaaaacgg ttatgacagc
     3001 agcggctttt gaacaactaa gtccactgca gcaggctatt tatgaacaag tgttaaaaac
     3061 aacggtggcc atgtttgcgg aaaagtatac ctatgaagaa acaacgatct tgacgcaagt
     3121 tcagcagctt cagttaaagg caatcgaaaa ggtgccgcta gatctaggtt ggaagaagtt
     3181 atttggaaaa gagagcgacg gaaaagagaa ggaagaagag ccgctacttc ctaaagtgac
     3241 aaaaggagaa atggtgacgg tggatctaca agttcttgaa aaagaaacca agccaccaca
     3301 accttatacc gaaggtacgt tgatcactgc aatgaaaaca gctgggaaaa ccgtggatag
     3361 tgaagaagcg caatccatat tgaaagaagt ggaaggaatc ggtacagaag cgacacgggc
     3421 gaatattatt gaaaccttga aacaaaagga atatatcaag gtagagaaaa ataagctcgt
     3481 ggtgacgaat aaagggattt tgctttgcca agccgtggaa aaagagccgc tgcttacgag
     3541 cgccgaaatg accgccaaat gggaaagcta cttgttaaag atcggtgaac gaaaaggaac
     3601 acaaaccact tttctagcca atatccaaaa atttgtctct c
//
LOCUS       DACGLW010000144         3522 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.152, whole genome shotgun sequence.
ACCESSION   DACGLW010000144 DACGLW010000000
VERSION     DACGLW010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3522
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.152"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            130..951
                     /gene="racE"
                     /locus_tag="IXT53_002837"
     CDS             130..951
                     /gene="racE"
                     /locus_tag="IXT53_002837"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HAQ4906596.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            954..2309
                     /gene="rph"
                     /locus_tag="IXT53_002838"
     CDS             954..2309
                     /gene="rph"
                     /locus_tag="IXT53_002838"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HAQ4906597.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            2310..2828
                     /locus_tag="IXT53_002839"
     CDS             2310..2828
                     /locus_tag="IXT53_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HAQ4906598.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            2935..3435
                     /locus_tag="IXT53_002840"
     CDS             2935..3435
                     /locus_tag="IXT53_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HAQ4906599.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         1199 a          595 c          800 g          928 t
ORIGIN      
        1 aagtcacaac cgcttttcct ctttccattc gaaaaaaaga gatttcgatt gttttaagca
       61 agtaagagca cttgaaaatt cacgctaaaa agtacacaat agggaggata gaatttattg
      121 aaaaaaagga tgatacgatt gacagataat cgccctatcg gatttattga ttcaggtgtc
      181 ggcggcttga ctgtagtaaa agaagccctg aaacaattac cgaatgaaaa tattttattt
      241 gtaggagaca cagcacgctg cccatatggc cctagacccg cggaacaggt aatccagtat
      301 acttgggaaa tgacggatta tctggtggag caaggaatca agatgctggt gatcgcctgc
      361 aataccgcaa ctgcggtggc tttagaagaa atcaaagctg ctctttctat tccagtcatc
      421 ggtgtgatcc ttcccggtac tagagcggca gtaaaaaaaa cacaaaataa acaagttggc
      481 attatcggta cgattggtac ggtaaaaagt caagcttatg aaaaagcact gaaagagaaa
      541 gtaccagaat tgactgtgac aagtcttgct tgtcccaaat ttgtttcagt tgtcgaaagt
      601 aatgaatacc attcatcggt ggcgaaaaaa attgtggcag aaacattagc tcctttaacc
      661 actaaaaaaa tcgatacatt gattttggga tgcacccatt atccattatt acgccccatc
      721 attcaaaatg taatgggaga aaatgttcaa ctgatcgatt ctggagcaga aacagtaggt
      781 gaagtatcta tgctgttaga ttatttcaat ttgagcaatt caccgcaaaa tggtcggaca
      841 ttatgccagt tttatacaac tggctctgcc aaacttttcg aggaaatagc tgaagactgg
      901 cttggaatcg gacacttaaa tgtagaacat atcgaattgg gaggaaaata aggatgcgtc
      961 atgacgggag aaaagcccaa gagcttcgca aactggagct tcaaacaaat gttttcaaat
     1021 atccagaagg atcggtagtg atccgttttg gtgatacaac agttatttgt tctgcaacaa
     1081 tagaaaattc tgttccccct tttttgagag aaacaggaac aggatgggtg acagcagaat
     1141 atagtatgct gcctcgagca acgagtacga gaaatcgtag agaaagcagc aaagggaaac
     1201 tttctggtag aacaatggaa atccagcgtc ttattggtcg ttccttgcgt gcagtagtag
     1261 acttggaaaa attaggagaa cgtagtatca tcgtagactg cgatgtgatc caagcggatg
     1321 gcggtactag aacagcaagt atcactgggg catttgtggc attgaaatta gccattgaaa
     1381 aacttttaag agaaaaagaa ttgtcagaag atccaataaa agaacatctt gctgcagtta
     1441 gcgtaggaat cttaccagat gggacttgcg tcacagattt ggattaccaa gaagattccg
     1501 cagcacttgt agacatgaat cttgtaatga cagaatctgg aaaattcgtg gagattcaag
     1561 gaactggaga agaagcaacg tttgatggtg aacagttgaa tgaaatgctc ttttttggaa
     1621 aaaacgcgat tgaagatttg atcaaagagc aaaaacatgc tttgctaaca gagtttgctc
     1681 aaaatgatga gcggatagaa gaaacaaaaa caatcatcat tgcgacaaga aatccaggaa
     1741 aagcagaaga gtttcgaaat atgttcaaag aagcgggata tcatgtaaaa acattacttg
     1801 actatcctga attgccagat gtggaagaaa ctggcagcac atttgaagaa aatgcccgat
     1861 taaaagcaga aacgattgca caattgcttg atcagccagt cttagcagat gattcaggtt
     1921 tgaaagtgga tgctttaggc ggtatgccag gaatctattc tgcacgtttt gcaggggaac
     1981 aaaaaagtga tgcggggaat aacgccaaat tattatatga attgacagat gtgccggatg
     2041 aaaaaagaac agcacaattc cattgtacgt tagtattcgc ggcacccaaa aaagacagcc
     2101 ttgtagtaga agcggaatgg cccggtcgag tagcccgtat accatctggc gaaaatggct
     2161 ttgggtatga tcctttattt atcccagaag gtaagaaaca aacagcagca gaattatcga
     2221 gcgaagaaaa aaacaagatc agtcatcggg ctcaagcaat gaaaaaattg agtgccgagt
     2281 ggaaacaatg gctggaagga gagagataga tgcggtattt agtagtaagt gataatcatg
     2341 gagacagaga tatcctcgtt gacttagtag atcgttatcg ttctgaagtg gatatctttt
     2401 tccattgtgg tgattcggaa ttagaagctt ctgacccatt atgggataca ttccaagtgg
     2461 ttcaaggtaa ttgtgattat ggaccaggat tcgaacagaa aaaagtgatc caaactggtc
     2521 aggatacagt attcatgact cacggtcatt tatcgaatgt ccgatttggt ttgacacagt
     2581 tagcaatcga agctcgagct gcaactgccg atatggtgtt ttttggtcat acccatcaaa
     2641 taggctgtga gatggatcag aaaatcctgt ttttgaatcc tggtagcatt tctcagccta
     2701 gaggcccgat ccaaatccca tcatatgctc ttatcgaaag tttagatgac caaatttctg
     2761 ttcaatacta taaccgtgcc catcaggcag ttgaagagtt ggccttttct ttttcgagag
     2821 agcgctgaat aaaaaaactc taatttttaa taaaaataag ctttatttta tgaattttgc
     2881 atccggtttc ggtaaaatag tagtgttgaa tgaaatagaa atggaggcca tcagatgatt
     2941 ggacctatag taagagaaat gctgctggaa aatcaggaaa cgtttttgat tcctgcagaa
     3001 aatgtggcaa atgttatgta tcatcatcca ttgtcccatg gtttacttgt gttatcgaag
     3061 gtcggttatt ccaaaattcc tgtattagga aaagatgatc actttgtagg actagtaagt
     3121 ttatcagatg tggtcaataa aatgattgac ttgcaaacaa tcagcatgga cccgttagaa
     3181 ggactaacgg ttgctgatgt gacagaaaca gatgttccga caattgatga aaattgggaa
     3241 ctagaagaag tactccattt gttagtcgat tcgccttttt taccggttgt cacagaagat
     3301 cggattttca aaggaatcat tacaagaaaa gagctgctta aagccgtaaa cttcatggtg
     3361 catgaattag aacgtcgtaa tatattgtct ccgaaaatcg aagaattaaa agaacaaatc
     3421 gatatcgtga gctaaagaac gagaaactaa aagataatca aaaaataagt agaaaaaaca
     3481 gcgtaccagt tttgacttta tcttgaaaaa gaggctggga ca
//
LOCUS       DACGLW010000145         3463 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.153, whole genome shotgun sequence.
ACCESSION   DACGLW010000145 DACGLW010000000
VERSION     DACGLW010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3463
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.153"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            137..748
                     /locus_tag="IXT53_002841"
     CDS             137..748
                     /locus_tag="IXT53_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HAQ4906600.1"
                     /translation="MYFILPTLYSLLCVMAPCIIYMVFFCKKNTLKHNILILIFMLYC
                     WKVFELTGTGGLTDIFYRPDTGRKFSLVQGSVNLVPFSGLNISFILNIVMCVPLGFLL
                     PIIWKSYRKIVPVFLAGALFSLIIEVSQIITTRATDIDDLIANTFGAVIGFILWAVLS
                     RVVSLKDKSSTVDYLEPYIYILIGFTGKFLLYFPFWFAFEVLS"
     gene            821..1750
                     /locus_tag="IXT53_002842"
     CDS             821..1750
                     /locus_tag="IXT53_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HAQ4906601.1"
                     /translation="MKRFLKWGLVLLASLIVILMCTAVVISYVNRPYARAVYDRIKRI
                     LDKDFYDHLYLGSSFEIINLDTMDIHSYMKKDQEELPASLSKLFTIDYALSVLSLDEQ
                     ITISPTALDMQKADSSTAHLQEGDILSVYDIICAMVLPSGNDAAYSLAIYTGGKLDPD
                     AKNDEERYQSFMKNLDRYLSEQGYKHAHFTDAAGYDSNATTSAEDLTKVAAKLLKNDK
                     FAEIVAKETYRAKSIKGNVYNWNTTNQFMQQDNRYFTEGVKGVKTGSFDGRYNLCVYY
                     TSSSDNHYLLVSFGSESDSRRYVDVQNLLHNLP"
     gene            1908..2645
                     /locus_tag="IXT53_002843"
     CDS             1908..2645
                     /locus_tag="IXT53_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HAQ4906602.1"
                     /translation="MLISCGARLAPFDIRELRELMSEDELELDKIGDRKTALFVIISD
                     TDDTFNFVVSIMYSQLFNLLCDKADDVYGGRLPVHVRCLLDEFANIGLIPKFEKLIAT
                     IRSREISASIILQAQSQLKAIYKDHADTIVGNCDSTLFLGGKEKTTVKELSETLGKET
                     IDLYNTSETRSNQKSFGLNYQKTGKELMSQDEITVMDGGKCIYQLRGARPFLSDKFDI
                     TKHKNYKLLEDYDKKNLFDVEEYLKQR"
     gene            complement(2693..>3463)
                     /locus_tag="IXT53_002844"
     CDS             complement(2693..>3463)
                     /locus_tag="IXT53_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ4906603.1"
                     /translation="MTKSFTEEVTSFKERELHDRYAAIYMDATYIPLKRKTVAKEAIH
                     IAVGIRPDGSKEVLSYAIAPTESITIWEEILIDLQERGVKNVLLFITDGLKGMAGAVQ
                     RFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMVYQASSKKAALEARGAFAEKW
                     KTSYPKMVESILSNDYLLTFYDFPLAIRKTIYSTNLIESFNKQIKKYSHRKEQFQNEE
                     SLERFLVSIFDTYNQKFLNRSHKGFQQVTDTLASMFTE"
BASE COUNT         1148 a          538 c          661 g         1116 t
ORIGIN      
        1 cctctaggaa aaaattaact agcaccacgg gttatgatat aataaaatgt attataggca
       61 tttttttaca agaaatagaa gcttgaagtt tatatgaagt tataaaattt aatttatatt
      121 ggaaggagga aaataaatgt attttatact acctacactt tatagccttt tatgtgtaat
      181 ggcaccatgt attatttata tggtattttt ttgcaaaaaa aataccctga aacacaatat
      241 cctgattttg atttttatgc tgtactgttg gaaagttttt gaattgactg gtacaggtgg
      301 actaacagat attttttata gacctgatac aggtaggaaa ttttctctag tacagggcag
      361 tgttaatctt gtaccattca gtggactaaa cattagcttt atattaaata tagtaatgtg
      421 tgtgccctta ggattcttac taccaatcat atggaaaagt tataggaaaa tagttcctgt
      481 tttcttggct ggggctctat tttcactaat tattgaagtc tcacaaatta taactacaag
      541 agcaacagat attgatgatt taatagcaaa tacctttggt gcggttatag gctttatact
      601 gtgggctgtt ttaagtagag tggtgtcctt gaaggacaag tcatctacag tagattattt
      661 agaaccttat atttatatat tgattggttt taccggaaaa ttcttactgt attttccttt
      721 ctggtttgct tttgaagtat tatcctgata attccataga aaataatgag tttgagttga
      781 gaaagaatat tcttgtacac aactctggaa aggaggaagt atgaaaagat tcttaaaatg
      841 ggggcttgtt cttttagcgt ctttgattgt gattctaatg tgtacagcgg ttgttatttc
      901 ttatgtgaat cggccgtatg caagagctgt ttatgatagg attaaacgca ttcttgacaa
      961 agatttttat gaccatctat atctaggttc ttcatttgaa ataataaacc ttgatacaat
     1021 ggatattcat agctatatga aaaaagacca agaagaatta cccgcctcat tgagcaaatt
     1081 atttacgatt gattatgcgc ttagtgtcct aagtttagat gaacagatta caattagtcc
     1141 tactgcactt gacatgcaga aagctgactc atctactgca catcttcaag aaggagatat
     1201 tttatctgtt tatgacatta tctgtgctat ggtcttaccc tcaggaaatg atgcagctta
     1261 tagtttggca atttatacag gtggaaaact tgatcctgat gcaaagaatg atgaagaacg
     1321 ctatcaaagc ttcatgaaaa acttagatcg ttatctaagt gagcaaggct ataaacatgc
     1381 tcattttact gacgctgctg gctatgactc caatgctaca acatcagcgg aagatttgac
     1441 aaaagtagct gcaaagctac taaagaatga taaatttgca gaaatagttg ccaaggaaac
     1501 ttatagagca aaatcaatta agggaaatgt ttataactgg aatactacga atcaatttat
     1561 gcaacaagat aatagatact ttactgaagg tgtaaaaggt gtaaaaactg gatcgtttga
     1621 tgggagatac aatctatgtg tgtattatac atctagttct gataaccact atttacttgt
     1681 aagttttggt tcagagagtg atagtaggcg ttatgtagat gttcaaaatc tattgcataa
     1741 tctgccttga aatttcgtgc ctttaagcgc gtatctaaag tttctttgaa aagtttgaga
     1801 atgaagaaag tgagcagtcg aaaaacggtt gctcactttt ttgccctaaa atcgaaaaaa
     1861 tataaaaaag tcctttacaa cacgtctcag gaaaaactgc taagtcaatt ctaatatctt
     1921 gtggagcaag acttgcacct tttgatataa gagagcttag agaactaatg agcgaagatg
     1981 aactagaact cgataaaatt ggagatagaa aaactgcctt gtttgtaata atatcagata
     2041 cagatgatac atttaatttt gtagtttcaa taatgtattc tcaattattt aatctactat
     2101 gtgataaggc agatgatgtg tatggtggaa gacttccagt tcatgttagg tgtctacttg
     2161 atgagtttgc aaatatcggt ttgattccta aatttgaaaa actaatagcg acaattcgtt
     2221 caagagaaat atcggcaagt ataatactgc aagcacaatc tcaattaaaa gcaatttata
     2281 aagaccatgc agatacaata gttggtaact gtgactctac actttttcta ggaggaaaag
     2341 aaaaaacaac agtaaaagaa ttatcagaaa ccttaggaaa agaaaccata gacctatata
     2401 acacatcaga aacaagatca aatcaaaaat cttttggtct aaattatcaa aagactggca
     2461 aggaacttat gagtcaagat gaaataactg taatggatgg tggtaagtgt atttaccagc
     2521 taagaggagc aagacctttt ctatctgata aatttgatat tacaaagcat aagaattaca
     2581 aattgttgga agattatgat aagaaaaact tgtttgatgt agaagagtat ttaaaacaaa
     2641 gatagcgtaa aatattttgt gtaaataact cgtccttctg caaaataatg ggttactcag
     2701 taaacattga agctaatgta tccgtcactt gttggaagcc tttatggctt ctatttaaga
     2761 atttttgatt gtatgtatca aagatggata ctaggaaacg ctctagtgat tcttcatttt
     2821 gaaactgctc ttttctgtgg ctgtatttct tgatttgctt attaaaggat tcaatcaagt
     2881 tcgtagagta aatagtcttg cgtatggcta atggaaaatc atagaaagtg agcaggtaat
     2941 cgttcgaaag gatcgattcc accatttttg gataactggt tttccatttt tccgcaaaag
     3001 caccacgtgc ttccaatgcc gcctttttag atgacgcttg atacaccatt ttaaaatcat
     3061 cacagacttc cttacgatca tcgacacgca ctttgtgact gatattacgg gaaacgtgca
     3121 cacagcaatg ttgaaaacgg gctttggggt agaaccgctg cacggcccct gccatcccct
     3181 ttaagccatc cgtgatgaaa aggaggacgt ttttcacgcc acgttcctgg aggtctatta
     3241 aaatctcttc ccaaatcgtg atagattcag ttggtgcaat cgcatagctc aacacttcct
     3301 ttgaaccgtc cgggcgaatg ccaactgcga tatgaatggc ttccttggcg acggttttcc
     3361 gctttaaagg gatataggtt gcgtccatat aaatagcggc ataacggtca tgaagctcac
     3421 gttccttaaa tgaagtgact tcttccgtaa atgatttagt cat
//
LOCUS       DACGLW010000146         3199 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.154, whole genome shotgun sequence.
ACCESSION   DACGLW010000146 DACGLW010000000
VERSION     DACGLW010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3199)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3199)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3199
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.154"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            668..763
                     /locus_tag="IXT53_002845"
     CDS             668..763
                     /locus_tag="IXT53_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HAQ4906604.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(1065..1310)
                     /locus_tag="IXT53_002846"
     CDS             complement(1065..1310)
                     /locus_tag="IXT53_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906605.1"
                     /translation="MRLEKEKMEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNM
                     TPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
     gene            1655..1939
                     /locus_tag="IXT53_002847"
     CDS             1655..1939
                     /locus_tag="IXT53_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906606.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            1942..2358
                     /locus_tag="IXT53_002848"
     CDS             1942..2358
                     /locus_tag="IXT53_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906607.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(2495..3172)
                     /locus_tag="IXT53_002849"
     CDS             complement(2495..3172)
                     /locus_tag="IXT53_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HAQ4906608.1"
                     /translation="MNLVPLKNFNAKQMDLFFALCARMKDRGLKKVRFSFEELKELSD
                     YKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEINKTKKFVEVSVNPD
                     LEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYYVVRIEEFRSLMDIP
                     EYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLEFTFSSLTSDLPKIS
                     LRNWLEE"
BASE COUNT         1065 a          597 c          499 g         1038 t
ORIGIN      
        1 cgttttttca tccattcctt tcttcccccc ttacaaataa ataataacct ttaacaacca
       61 actcgtaaat aggttggttg ttattataca aaaggatatt agtactcaga taaaaagttg
      121 ttagtactca gataaaaagt tgttagtact cagataaaaa gttgttagta ctcagataaa
      181 aagttgttag tacccttctg tatccgttgg gagagtaagg ctcaactgcc ccctaaaaag
      241 ggttctaaaa aatctttaaa aaataaataa aataaacgcg cgcgcgcgag caaaatttcc
      301 cgccaaaaaa tcacccactt tttaaaataa acaggttcgc gcatgatatt aaactgccaa
      361 tctagctccc tatggtcgct gtaagaagga gtgatcgcta cgctctcaca ttttacttgc
      421 agcagtcgcc tttaggctcc ttcgccaaaa ataattcatt agggactcct ttgtggcgtc
      481 ctgagagctc gtctaagccg ttctaagaca agattcgata aaaactatgt ccttcgttct
      541 aaagcgcctt acaggcggtc agggcaagcc ctaacaaccc caaggcaata aaaaacttag
      601 gcttattttt gcttttttat tgtataattg attgtcgcct atccgacagt cttgcgactg
      661 gaaggaggtg tgtttcgtgt tcactaactt tatttgctcc cttcttgttg ggcttttaag
      721 cgctttgttc agttactggt tgaataaccg aaacaagaag taaaacttaa ttaaggcgac
      781 tcagcccaca aaaaaagccc cagagaatcc gacctctctg gggctttcgt gtgtttcgtg
      841 ttcacttatt tgcaatcaaa ttataacaga aataaatatt tatttcaaaa atatcctaca
      901 aaagtctaaa atccgtaatt atttcttata tgcaacattt acaaataaag tcgctctgag
      961 agctcgtatt tttcgattta agacgtttta tttagttttt ggtgtatttc attggtccaa
     1021 gctaaaaaac tcttagaagc gattttagag ccatttaaac ccacttaatt atattctcca
     1081 tgaaattcgc tccatgcgtc aaaagctgcg tctgaaattc cgccgtcttc gttcactagt
     1141 tgcattaatt tttccaacga tcgttttctt tcgggtgtca tatttttata ttccaacaat
     1201 ttttcgtctg ttaaatcgaa cttgattgct tgaattgctt gctctaattt ttctaagttt
     1261 tgaaaatatt cgtaaggaaa tttttccatt ttttctttct ccagtctcac aatttttcta
     1321 gaaataaaat cacttctttg ccgtctatac tctcaaaaaa aatgtcgttt gaaaaactaa
     1381 ataaattttt gatcgtgcat ttttaaattt ggttatcgat tacacgtgca gcactccccc
     1441 ccgaatttta aagagcataa ggaaacgtgt acccactttc taaaaaaagt gggtagatag
     1501 gctgtgccta tcacgtttct cagaaaaaaa tttgatatca ctttttcgcc atcgtgggta
     1561 gcttatgtat agcattttgg caaaaaacag ctatacaaat atagtatttg tatggtatac
     1621 ataaattata cataattgca aaaggagatt ttttatggac attttagttc ggaatattga
     1681 tccaaagttt gttgcaacaa tagacaaacg ttgccaagaa ctcacgaata aaacaggaga
     1741 aaaatggagt cgaaataaat atctaaaagt tttaatcgaa agtgattttg accgtcaact
     1801 tacgacttac aaaaaagacc aattcgattt attgctcgat aaatttattg ctatccaaac
     1861 agaaaatact caaatattaa gcgagtatgt ctacacaaat aatcaaatca ttgaaatgct
     1921 tatcggagga atgatataac catgagaaaa acttctgtcc aaaaacatgt tactttgtac
     1981 cctagtcatg ttgacgtttt agattccatc atcgaaaaaa atccagatat caataactac
     2041 tcggaagcca ttcgcttttt atgtatgagt tatgaacccg ataccaacac gaccactacc
     2101 agcaatcagg caaaactaaa tgctatggga aaagaaattt ccatcatgac tgaaatcatc
     2161 gcagaatttg ctgatttata tctaaaagat acaggcgttc ttattggcac aaattctaaa
     2221 gtatatcaag aagtgaaaga aaaaatcgaa gctaaactaa atccatcaaa tcgaaatcga
     2281 ccaaagaatc atcaaaaaga aaccagtcaa acagtcactt ctagaaattt cgacccaaca
     2341 aattttctaa aacgataaaa aacagatagg agttattcct atctgttttt tatttagaag
     2401 ccccatacaa tatgctagaa tgtactagca atcttagtcg gttgtaaggg acttgtctac
     2461 ttgcttaatg taaggggggc gagttctttt ttatttattc ttctaaccag ttacgcagag
     2521 aaatctttgg caaatctgac gttaggcttg aaaaagtaaa ttctaatttg gcgattttat
     2581 tgcccttttt tgcttttatt tttgtgactt taagatcatt gaagtatttt tttagttctc
     2641 gtaatgcagg atttaatact ttttgatcaa tgttacccat ttgatagtat tctgggatat
     2701 ccattaagct cctaaactct tcaatacgaa ctacataata accagtagaa cggtactgca
     2761 taagcagtcg gaatagagtt tttgcatacg tagaacgcaa cgacgtgaaa gcagataatt
     2821 caaatctact aaattcagct gttaaaccat taataatata ttctaggtcc ggattaacac
     2881 tgacttcaac aaactttttt gttttattga tttcaaaacc agtaaacaaa acaaagtagc
     2941 gaatagaatc ctcatcttca gtacggtaag taagattaag catatcttta tagagctttt
     3001 ctaaatcacc gacaaaggct tttgtagcag tcatcttata atcacttaac tcttttaatt
     3061 cctcgaaaga aaatctaact tttttcaaac cacgatcttt catacgagcg cataatgcaa
     3121 aaaataaatc catctgctta gcattgaaat ttttcagcgg tactaaattc aattcatttt
     3181 gatatttgac tgttaattc
//
LOCUS       DACGLW010000147         3066 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.155, whole genome shotgun sequence.
ACCESSION   DACGLW010000147 DACGLW010000000
VERSION     DACGLW010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.155"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            complement(333..695)
                     /locus_tag="IXT53_002850"
     CDS             complement(333..695)
                     /locus_tag="IXT53_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906609.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(769..1149)
                     /locus_tag="IXT53_002851"
     CDS             complement(769..1149)
                     /locus_tag="IXT53_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HAQ4906610.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1231..2406
                     /locus_tag="IXT53_002852"
     CDS             1231..2406
                     /locus_tag="IXT53_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HAQ4906611.1"
                     /translation="MIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADMDFLSPG
                     GVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVLTSLAAA
                     VQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDMEKKIEEN
                     NVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHTFTSMLT
                     IDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEINTFGLI
                     GTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLDFTAYIS
                     DDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYALETID"
     gene            complement(2483..>3066)
                     /locus_tag="IXT53_002853"
     CDS             complement(2483..>3066)
                     /locus_tag="IXT53_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HAQ4906612.1"
                     /translation="AFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIELS
                     KKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMAN
                     SGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHVL
                     EGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
BASE COUNT          956 a          587 c          504 g         1019 t
ORIGIN      
        1 acgactcagt tatcttatca tgtcgttagt aacatgtcaa taactatttt ttaaattttt
       61 tagaaatgtg atataacatc cacttcagaa tcaacgctgt taaatttatc atgtatttct
      121 tccttggtca actataaatt aagcattact ttgtgaaatg atttttcgat caattattat
      181 tattcgttat acaaccgttt tcaacatatt ttgaaaataa aatgcactaa aaaactatct
      241 ttagcagaac atttcattgt attttttgga ccaaacaata aaaaaagaag tttgaaaaaa
      301 attcaaactt ctttgttcgc ttaatctgta ttttagaaga aaggcactcc ctctacttcg
      361 taagttccaa gaacagagcg catttgacct ggttcttccc aaatatatcc ttcgcctaat
      421 tcttctcttt gattcttttg ttcagtttca aagacaaaca acggaacatt ttcctcttta
      481 atatgtgtat ttgtcaattc catcaaacta atcttgctaa catcgatatg aactatttct
      541 tttaagaaat taagtatatt cgctaaacca gtctttcctt cagaaagttc cgctaacgca
      601 aattccaact tgtcatcaat tgaatggacc aaaaattttt tagtaccatc ctccaaatgc
      661 aacataattg taccagcaac tcttttgctt gccaaaattt ccacctcgat caacttgttc
      721 tatggcttac ataaaaattc tagcacattt tcagaaaaaa tacacttttt atttcttatt
      781 ttctttccgt tctagttcag ataatgcttc ttctatccaa ataggtaact gctccgcaat
      841 agtggtaaat ccgatcttac gatttttatt cgtgaaatac ctttttctgc gatatggaac
      901 agctggattt gttttttcta acgttcttaa agataaactg atttttttgg aatattcatc
      961 aatgtcgatg atctgaactg ttactggctg tccgactttt aacaatgaat ggatattttt
     1021 cgtataaccg gattgtactt ctgaaacatg aataagtcct tgcgtctcgt tatcaagaga
     1081 aacgaatgcg ccataaggct gtatccctgt cacttttcct tcaattattt gtccaatttt
     1141 ataagtcatc tacttccctc tcttcgcatt tatcattata ctgtaaatag ctcaaaaaaa
     1201 gaagcgacga aaatgactgg aggtcttaag atgatcgaat ttgataaaca gatttcaaga
     1261 aaagatacag aaagtgtaaa atgggacgca attgctgaga catatcaaat ggatgactta
     1321 cttcctttgt gggtagcaga tatggacttc ttgtctcccg gtggggtagc taaagcgttt
     1381 agtgaataca tcaaacatgg aatctttggt tatgccactc tgtctgataa actatatcag
     1441 gctatcattc attgggaacg ccaacaccat gctgtggagt tgacgaaaga aaatatcgtt
     1501 tttacaagtg gtgtgttaac cagtttagct gcagcagtac aaacttttac aaaaccaggc
     1561 gattctattt tgattcatga cccagtttat cctccatttt cctctattat agaaacaaat
     1621 caacgtcaca tcgttcgaag ctctttgcta gaaaaaaata atcatttcat catggacttg
     1681 tctgacatgg agaaaaaaat cgaagaaaac aatgtaaaag caatgatttt atgcaatcct
     1741 cacaatcctg gagggcgtgt ttggagcaaa gaagaattga aacagttaag cgaattgtgt
     1801 ttaaaacatc aagtgattct gtttagtgac gagatccatc aagatttaac tttattcgat
     1861 catacattta cttccatgct aacgattgat cctaatttag atgatctact agttgctttt
     1921 acttccgcaa ccaagacttt caatctagct gcaatcaaaa attcaatggt atttatcaaa
     1981 aatccagaga tgaaagctga ttttgaaaaa catttagtga tgaaccaaca gcacgaaatc
     2041 aacacttttg gactgattgg aacccaagcg gcttatgaga cgggtggaga atggttaagc
     2101 caactgattc cttatctaga aaaaaatgtt caaacagtta atcagttttt tgaacagtat
     2161 cttccgaaag tgagaataat gaaccctgaa ggaacttact taatgtggtt agattttacc
     2221 gcctatattt cagacgacca agaattggag aacactcttg ttcggaaagg aaaagtcgta
     2281 ttaaatcctg gcatcacgtt tggtccttcc ggtcatgggc atatgcgctt gaatattgct
     2341 tgtccagaag aaactttact ggaaggactt aatcgaatca agtatgcttt agagacaatc
     2401 gattaaatca tttattcaaa ataaaaggac gagaccttac taggtttcgt cctttttcat
     2461 aattactccg taccatacca aactattctg aaatttcaat tgtttcaatc actacatcat
     2521 gtacaggtcg gtcttgtgga ccacgttgta catttgcgat ttcatctact gtgtccatcc
     2581 cttctagtac atgtccaaag actgtatgtc ggaaatctag ccaaggagtt ccgccacctt
     2641 tatacgcttc aatgatttct gatgggaatc cagcaccttc tagttgtcca agcatattag
     2701 ctggcacatt tgtgttattt acaataaaga attggcttcc gtttgtgttt ggacctgaat
     2761 ttgccataga taaagctccg cgaaggttga acagttcacg actgaattca tcttcaaaac
     2821 tttcgccata aatactttcg ccacccattc ctgttccagt tggatcgccg ccttggatca
     2881 tgaaatcagg aataacgcgg tgaaaaatca cgccatcata ataacctttt ttggaaagtt
     2941 caatgaaatt ttgaaccgtc ttcggtgcta gttcagggaa taattgaaca gtaatgtcac
     3001 cacggtttgt tttgataaca gcttttggtc ctttttcact ttttaaatct agttgtggga
     3061 atgcca
//
LOCUS       DACGLW010000148         2951 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.156, whole genome shotgun sequence.
ACCESSION   DACGLW010000148 DACGLW010000000
VERSION     DACGLW010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.156"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            117..299
                     /locus_tag="IXT53_002854"
     CDS             117..299
                     /locus_tag="IXT53_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HAQ4906613.1"
                     /translation="MINLFLLVTASTLFFTGISLIISYLIYKKAENHTSKAIGLAVSS
                     FLTLLTLFVTLSFSFV"
     gene            402..686
                     /locus_tag="IXT53_002855"
     CDS             402..686
                     /locus_tag="IXT53_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906614.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGIVKQLIKDREFKKFNSRQAYN
                     RQLKKAILKHKDSFIGNLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            702..938
                     /locus_tag="IXT53_002856"
     CDS             702..938
                     /locus_tag="IXT53_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906615.1"
                     /translation="MEGKTFAEIWTEFIEVPTRIDPITSEKVLDADFYNLKAGTSLEC
                     VWMYLEEHFKGVTLGHVLYHNGAEWDAIGNIHFS"
     gene            960..1214
                     /locus_tag="IXT53_002857"
     CDS             960..1214
                     /locus_tag="IXT53_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906616.1"
                     /translation="MNYTDEEQIALEYVFTNEEGEEIEKVLIISSKDLEKALAYEPYE
                     STVTVIEFLDTYTSNDTWEIMNICNANNIQFAEKTNKSFY"
     gene            1296..1481
                     /locus_tag="IXT53_002858"
     CDS             1296..1481
                     /locus_tag="IXT53_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HAQ4906617.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            1548..1943
                     /locus_tag="IXT53_002859"
     CDS             1548..1943
                     /locus_tag="IXT53_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin HicB"
                     /protein_id="HAQ4906618.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEELLEK"
     gene            1933..2310
                     /locus_tag="IXT53_002860"
     CDS             1933..2310
                     /locus_tag="IXT53_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906619.1"
                     /translation="MKSKKETEPMKATHLNYFSRTLINNLLKDTTGISVSTKHFANDL
                     IIFEDNNLRIEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKK
                     AKLLFEFQTDYHNELLAQSEILD"
     gene            2311..2706
                     /locus_tag="IXT53_002861"
     CDS             2311..2706
                     /locus_tag="IXT53_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906620.1"
                     /translation="MKQEKRKMKQTQTETVNDTLADQLAGYYKLFEMGKAQRLIHYLW
                     KYKILFLFWESTLTFSVLFYKSDFYNQHLVLGSFLLIYTILMLNLYTYITLEEHLVIS
                     DAGIERNIKRVSFKITLNKPTDKKLSEAF"
BASE COUNT         1038 a          502 c          515 g          896 t
ORIGIN      
        1 ttttctgggc tttgttttta gggtagggct aaaaaaaaca tcaccatctg atccaaaaca
       61 gctcagaaac gcatttaaac gtatctgata ccaatattac agaaggagtg tatcgtatga
      121 ttaacctatt tcttttagtg acagctagta cattattctt tactggaata tcactgataa
      181 tttcctattt gatctataag aaagccgaaa atcacacaag caaagcaatc ggactagccg
      241 tttcatcctt tttaactttg ctgactttat ttgttacttt atcgttttct tttgtttaga
      301 acagctgatc cattagcacc tttttctctt tacattatac acactgttgt gtataatgta
      361 tttgtaaaca agctaattaa cttttaggaa ggagtgacaa gatgctaaca gctactttac
      421 agcaacctac tcacgttgct attgcagaaa gttatacaat taatgcaggg atcgttaaac
      481 agctgatcaa ggatcgagaa ttcaaaaagt ttaacagtcg gcaagcgtat aacagacagc
      541 taaagaaagc tattctaaag cacaaagatt ccttcatcgg gaacttggaa agtgtagatg
      601 ttaaaactgt ttcaacggca ttttcagttg ttgaagtgac tattgacggt cgcttgattt
      661 ctctatttgc ttttggaatt aattgatagg gagtgtgaac tttggaaggt aagacttttg
      721 cagaaatttg gactgaattt attgaggtac caactcgcat tgatccgata acttctgaaa
      781 aggtattgga tgcagacttc tacaatctca aagcaggtac tagccttgaa tgcgtttgga
      841 tgtatttaga agagcatttc aaaggcgtaa cgctcggtca cgtcttatac cacaatggtg
      901 ccgagtggga tgcaattgga aatatccatt tttcttaata taagaaagga gaacacaata
      961 tgaattatac cgatgaagaa caaatcgctt tggaatacgt tttcacaaat gaagaagggg
     1021 aggaaattga aaaagtcttg atcatttcat caaaagactt ggagaaggct ttggcttatg
     1081 aaccatacga atcaaccgta actgtcattg aattcttaga tacctatact tctaatgata
     1141 cttgggaaat tatgaatatt tgcaacgcta ataacattca atttgctgaa aaaaccaata
     1201 aatcatttta ttagttttgc aataaaacat cctctttttc tattgattcc atacacaaca
     1261 atgtgtataa tagattttgt aaagggaggg tagctatgcc taagacagca agagaaattg
     1321 taaaaattct caaatctaac ggcttcaaaa agactggaca aactggcagt catgcaaaat
     1381 tttataattc caagactaaa agaacggtcg ttgttccgat ccacaatggt gatgtaccac
     1441 taggaacaga agctaatatc tggaagcaag cagggcttta acagccttgc cttcctattt
     1501 ggtattaggc atactatcct ctcttacact agaaaaggag cgaggaaatg aagacaaaaa
     1561 atatttttta tccggcaatc ttcgagaaag aaggagaagc ttataacatc gttttccctg
     1621 acttaccgga aatatctacc tttggaaata gtcttgaaga agcatattta aacgctaagg
     1681 atgctttagg attagcgcta tatagtgtac cagatgcaga atttcctaaa ccatcagcag
     1741 tggaagaatt aactttgaaa gccaaccaag tggtagtaat catccaacta aacattaaac
     1801 ttttccgaag aagcttaaat acaaaaacaa ttagaaaaaa tgttagtgtt ccggaatggt
     1861 tagttctttt agggaaggag aaaaacataa acttctctca actacttcaa aaagcactag
     1921 aagaagagtt gcttgaaaag taaaaaggag accgaaccta tgaaagctac acacttaaac
     1981 tatttctctc gaacgttaat caataatcta ttaaaagata caactggcat tagcgttagt
     2041 accaaacact ttgccaatga tctaattatt tttgaggata ataacttgcg aattgagatg
     2101 tttattgaac aggcaacccc taactcaata cctgaattct gctatacgca cactgaaaaa
     2161 atgaaacatg caaaatcagt taaagatgtt gaagcattaa gtgaatcact aacgaacgct
     2221 ttaaacctcg ttaagaaggc aaaattgtta tttgaatttc aaaccgacta ccacaatgag
     2281 ttgcttgctc aatctgaaat tttagattaa ttgaaacagg agaagagaaa gatgaaacaa
     2341 acacaaacag aaaccgttaa cgatacgctt gctgatcaat tagcaggtta ttataaatta
     2401 tttgagatgg gtaaagcaca gcgcttgatc cactatcttt ggaaatacaa aattttgttt
     2461 cttttttggg aatcaacact aacattctca gttctttttt ataagtcaga tttttataat
     2521 caacacttag ttttagggag ttttttgctt atctacacca ttttgatgct taatttatat
     2581 acctacatca cacttgaaga acacttagta attagcgatg cagggatcga gagaaacatc
     2641 aaaagggtat ccttcaagat cacgttaaac aagcctaccg ataaaaagct ctcagaagcg
     2701 ttctgagagc ttttgacatt ttcttgtata attttatcct aacacattac aaagcaccta
     2761 attcgagctt atatcaagca aatttgctat ttattactca atacatatct aaaaattact
     2821 tgatggaaaa ttagatacat ggtaagcatg aaaagaaaat caatttgagc agatgcagta
     2881 caatacatag caatttaaaa gctctcagaa ggcttctgag agcttttaat atattctttg
     2941 ataatttgtc t
//
LOCUS       DACGLW010000149         2868 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.158, whole genome shotgun sequence.
ACCESSION   DACGLW010000149 DACGLW010000000
VERSION     DACGLW010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.158"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            256..1224
                     /locus_tag="IXT53_002862"
     CDS             256..1224
                     /locus_tag="IXT53_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAQ4906621.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1217..2248
                     /gene="vanA"
                     /locus_tag="IXT53_002863"
     CDS             1217..2248
                     /gene="vanA"
                     /locus_tag="IXT53_002863"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAQ4906622.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2254..2862
                     /locus_tag="IXT53_002864"
     CDS             2254..2862
                     /locus_tag="IXT53_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAQ4906623.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT          845 a          549 c          702 g          772 t
ORIGIN      
        1 ttccagcgat gccagacttg gttgataaaa ggaggtccta agagatgtat ataatttttt
       61 aggaaaatct caaggttatc tttacttttt cttaggaaat taacaattta atattaagaa
      121 acggctcgtt cttacacggt agacttaata ccgtaagaac gagccgtttt cgttcttcag
      181 agaaagattt gacaagatta ccattggcat ccccgtttta tttggtgcct ttcacagaaa
      241 gggttggtct taattatgaa taacatcggc attactgttt atggatgtga gcaggatgag
      301 gcagatgcat tccatgctct ttcgcctcgc tttggcgtta tggcaacgat aattaacgcc
      361 aacgtgtcgg aatccaacgc caaatccgcg cctttcaatc aatgtatcag tgtgggacat
      421 aaatcagaga tttccgcctc tattcttctt gcgctgaaga gagccggtgt gaaatatatt
      481 tctacccgaa gcatcggctg caatcatata gatacaactg ctgctaagag aatgggcatc
      541 actgtcgaca atgtggcgta ctcgccggat agcgttgccg attatactat gatgctaatt
      601 cttatggcag tacgcaacgt aaaatcgatt gtgcgctctg tggaaaaaca tgatttcagg
      661 ttggacagcg accgtggcaa ggtactcagc gacatgacag ttggtgtggt gggaacgggc
      721 cagataggca aagcggttat tgagcggctg cgaggatttg gatgtaaagt gttggcttat
      781 agtcgcagcc gaagtataga ggtaaactat gtaccgtttg atgagttgct gcaaaatagc
      841 gatatcgtta cgcttcatgt gccgctcaat acggatacgc actatattat cagccacgaa
      901 caaatacaga gaatgaagca aggagcattt cttatcaata ctgggcgcgg tccacttgta
      961 gatacctatg agttggttaa agcattagaa aacgggaaac tgggcggtgc cgcattggat
     1021 gtattggaag gagaggaaga gtttttctac tctgattgca cccaaaaacc aattgataat
     1081 caatttttac ttaaacttca aagaatgcct aacgtgataa tcacaccgca tacggcctat
     1141 tataccgagc aagcgttgcg tgataccgtt gaaaaaacca ttaaaaactg tttggatttt
     1201 gaaaggagac aggagcatga atagaataaa agttgcaata ctgtttgggg gttgctcaga
     1261 ggagcatgac gtatcggtaa aatctgcaat agagatagcc gctaacatta ataaagaaaa
     1321 atacgagccg ttatacattg gaattacgaa atctggtgta tggaaaatgt gcgaaaaacc
     1381 ttgcgcggaa tgggaaaacg acaattgcta ttcagctgta ctctcgccgg ataaaaaaat
     1441 gcacggatta cttgttaaaa agaaccatga atatgaaatc aaccatgttg atgtagcatt
     1501 ttcagctttg catggcaagt caggtgaaga tggatccata caaggtctgt ttgaattgtc
     1561 cggtatccct tttgtaggct gcgatattca aagctcagca atttgtatgg acaaatcgtt
     1621 gacatacatc gttgcgaaaa atgctgggat agctactccc gccttttggg ttattaataa
     1681 agatgatagg ccggtggcag ctacgtttac ctatcctgtt tttgttaagc cggcgcgttc
     1741 aggctcatcc ttcggtgtga aaaaagtcaa tagcgcggac gaattggact acgcaattga
     1801 atcggcaaga caatatgaca gcaaaatctt aattgagcag gctgtttcgg gctgtgaggt
     1861 cggttgtgcg gtattgggaa acagtgccgc gttagttgtt ggcgaggtgg accaaatcag
     1921 gctgcagtac ggaatctttc gtattcatca ggaagtcgag ccggaaaaag gctctgaaaa
     1981 cgcagttata accgttcccg cagacctttc agcagaggag cgaggacgga tacaggaaac
     2041 ggcaaaaaaa atatataaag cgctcggctg tagaggtcta gcccgtgtgg atatgttttt
     2101 acaagataac ggccgcattg tactgaacga agtcaatact ctgcccggtt tcacgtcata
     2161 cagtcgttat ccccgtatga tggccgctgc aggtattgca cttcccgaac tgattgaccg
     2221 cttgatcgta ttagcgttaa aggggtgata agcatggaaa taggatttac ttttttagat
     2281 gaaatagtac acggtgttcg ttgggacgct aaatatgcca cttgggataa tttcaccgga
     2341 aaaccggttg acggttatga agtaaatcgc attgtaggga catacgagtt ggctgaatcg
     2401 cttttgaagg caaaagaact ggctgctacc caagggtacg gattgcttct atgggacggt
     2461 taccgtccta agcgtgctgt aaactgtttt atgcaatggg ctgcacagcc ggaaaataac
     2521 ctgacaaagg aaagttatta tcccaatatt gaccgaactg agatgatttc aaaaggatac
     2581 gtggcttcaa aatcaagcca tagccgcggc agtgccattg atcttacgct ttatcgatta
     2641 gacacgggtg agcttgtacc aatggggagc cgatttgatt ttatggatga acgctctcat
     2701 catgcggcaa atggaatatc atgcaatgaa gcgcaaaatc gcagacgttt gcgctccatc
     2761 atggaaaaca gtgggtttga agcatatagc ctcgaatggt ggcactatgt attaagagac
     2821 gaaccatacc ccaatagcta ttttgatttc cccgttaaat aaactttt
//
LOCUS       DACGLW010000150         2786 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.159, whole genome shotgun sequence.
ACCESSION   DACGLW010000150 DACGLW010000000
VERSION     DACGLW010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2786
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.159"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            54..656
                     /locus_tag="IXT53_002865"
     CDS             54..656
                     /locus_tag="IXT53_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906624.1"
                     /translation="MLKLISYAKKANSLSLVDNLDNLLPDKLIYDENEDNSQKQIIIF
                     ELYLKWLSSLSVSPESNDDDKYFANKLFQFIFPDYSSMPFFTLINFMLYVKEHLGYDD
                     LVEMIKAYDKEPYPFKGINTGSDMAMISGETVEEILANYKEQNIKTLELIKKNIKYYD
                     PLLANKENIDKLLNAIADSDVRSPRIDELKEEILMYKELF"
     gene            complement(653..1276)
                     /locus_tag="IXT53_002866"
     CDS             complement(653..1276)
                     /locus_tag="IXT53_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ4906625.1"
                     /translation="MKIAYIRVSSIDQNEQRQIEEMKKFGAEKIFIEKQSGATITHRP
                     IFQEALEFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PHLDKFIKDLIIQILAMIAEQERTESKRRQAQVIKIAKANGVYKGRPKLYSADTKNPQ
                     RRLVYENIVKDLNEGIAISKIAKNYNITRQTIYRIKNDIEYNSNQMN"
     gene            1594..1932
                     /locus_tag="IXT53_002867"
     CDS             1594..1932
                     /locus_tag="IXT53_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ4906626.1"
                     /translation="MNGIEDENLRRLINSLVIELYKYQAESERKKIKERQAQGIEIAK
                     KKGKFKGRQLKFKKNDPRLKHAFDLFLNGLSDKEVEEQTGINRRTFRRYRARYNVTVD
                     QRKNNEKRDS"
     gene            2024..2765
                     /locus_tag="IXT53_002868"
                     /pseudo
     CDS             2024..2765
                     /locus_tag="IXT53_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320596.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1005 a          427 c          483 g          871 t
ORIGIN      
        1 aatttttaga agaattatct ggagaagaat atagattatt cctaaataat aatattttaa
       61 aattaatatc ttatgccaag aaagctaata gcctatcttt agtagataac ttagataatc
      121 ttttacccga taaacttatt tatgatgaga atgaagataa ttcacaaaaa caaataatta
      181 tatttgaatt gtatttaaag tggcttagca gcctgtcagt gtcaccagaa agcaatgatg
      241 atgataaata ttttgcgaac aaactgtttc aatttatatt tccagactat tcttcaatgc
      301 cattttttac attaataaat tttatgctgt atgttaaaga acacttagga tatgatgatt
      361 tagtagaaat gataaaggca tatgataaag aaccttatcc attcaaagga atcaatactg
      421 gttcagatat ggctatgatt tcaggcgaaa ctgtagaaga aattctagct aattacaaag
      481 aacaaaatat aaaaactctg gaattaatta aaaagaatat aaaatactat gatcctttac
      541 tagcgaataa agaaaatatt gataagttat taaatgcaat tgctgattct gatgttagat
      601 ctcctagaat tgatgagtta aaagaagaaa ttttaatgta taaagaactt ttttaattca
      661 tttgattact gttatactca atatcattct taattctgta aatagtttgt ctagtaatat
      721 tgtagttttt agcaattttt gaaatagcta tgccctcatt taaatcctta acgatgtttt
      781 cataaactaa acgtcgttga ggattttttg tatctgcact atagagtttt ggacgacctt
      841 tatagacgcc atttgctttt gcaattttaa tgacttgcgc ctggcgtcgt ttagattcgg
      901 tacgctcttg ctctgcaatc atggccaaaa tttggatgat taaatcttta atgaatttat
      961 ccaaatgagg attgccaatt gcttcagcca tgatcggtag actggtgatg atcagctgca
     1021 cgtttttctt ctttaggtaa ttgactgaag caatgatttc gtcgtaattt cttccaagtc
     1081 ggtcgattgc ttcgacaata aaaatatctt ggtctcggac aaactctaaa gcttcctgga
     1141 aaatcggtcg atgagtaatc gttgctccag attgtttctc gatgaaaatt ttttctgcgc
     1201 cgaacttttt catttcttca atttggcgtt gctcattttg atcaatcgac gaaactcgaa
     1261 tataagcaat tttcaaaatt tttacctact tcctcaaact atttaaaata cactcaaaat
     1321 caacactaat ttttttatac actataaagc tgtcattttt ctagataaat ggcataaatt
     1381 ttcagcgcta agtgacaaaa gcaactaatt tttcatgata gatttttaaa tatctcaatc
     1441 tatctccaaa attagttgct tttgtagcta tagttgacta tgaatatgac aaataattag
     1501 aaaaaactgt gccaattaaa tatcaaaatg acaaattgat gaatcaaatt caaattaagg
     1561 ggggcaaccc tggaagtctt aaatttaccc tcaatgaatg gtattgaaga tgaaaattta
     1621 aggcgtttga ttaatagcct tgtcattgaa ttgtacaagt atcaagcaga atcagaacga
     1681 aaaaaaatta aggaacgtca ggcacaagga atcgaaattg ctaagaaaaa aggcaaattc
     1741 aaaggtcggc aactgaaatt taaaaaaaat gatccacgtt taaagcatgc tttcgatttg
     1801 tttttgaatg gtttatccga taaagaagtt gaagaacaaa ctgggatcaa tcgccgaacg
     1861 tttagaaggt atcgagcaag atataacgtg acagtagatc aaagaaaaaa caatgaaaag
     1921 agggatagtt aatgagtacg gttattttag ctgaaaaaca agccaggcat tagcctacgc
     1981 aagtgcttta aaacaaagca ccaaaaaaga cggttatttt gagatcaaag acccactatt
     2041 tacagatgaa acgtttatca cctttgattt tgggcattta gtggaattag cagaaccagg
     2101 tcattatgac gaaaagtggc aaaattggaa acttgaatct ttgccgattt ttcctgatcg
     2161 atacgatttt gaagttgcaa aagataaggg aaagcagttt aaaattgttg cagaacttct
     2221 caaaaaggca aatacaatta ttgttgcaac agatagcgac agagaaggtg aaaatatcgc
     2281 ctggtcgatt atccataaag caaatgcctt ttcaaaagat aaaacattta aaagactatg
     2341 gatcaatagc ttagaaaaag atgtgatccg aagcggtttt caaaatttgc aaccaggaat
     2401 gaattactat cccttttatc aagaagcgca aacacgccaa attgccgatt ggttgatcgg
     2461 catgaacgca agccctttgt atacgttaaa tttacaacag aagggcgtac aaggtacatt
     2521 ttcactagga cgtgttcaaa cgcccacctt ataccttatt tttcagcgcc aggaagctag
     2581 tataaaagta aaacaagggt catttaaggg cgttctaagc cccacacagc gttttaaaac
     2641 ccaagaggag cttttttgct tttgtttctt ctaaacaagc taaaataggc aatcaagagg
     2701 ggataattgc tgatgttcaa accaaagaga tggataggct acaattaact agacagaaaa
     2761 attaaggtgt gtagactaga agaaaa
//
LOCUS       DACGLW010000151         2718 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.160, whole genome shotgun sequence.
ACCESSION   DACGLW010000151 DACGLW010000000
VERSION     DACGLW010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2718
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.160"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            700..2610
                     /gene="ltrA"
                     /locus_tag="IXT53_002869"
     CDS             700..2610
                     /gene="ltrA"
                     /locus_tag="IXT53_002869"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HAQ4906627.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
BASE COUNT         1064 a          420 c          556 g          678 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaagacaa gctggtatca
     1861 caggcaaaga aaattgcttc tccctcaaaa gggaaaacgg aattagaaga aacaagactt
     1921 tataattcaa tggtattagg tatgcaaaat tattatcaga ttgcaacgaa tgtaaatctg
     1981 gattgtatga aactgaatag agctgtaatg gctatcttca ctaacagatt aggtacaaac
     2041 aaaaggggca gattgagaaa aacaggcaga caacttacga caaaggaggc aacaagatat
     2101 ggtaaatcta aaatgcttcg gtatgttgct ggcgagggcg aacctatttt ccctgttgga
     2161 tatatccaac acagaaaacc aatggcaaag gtgtatttag cgaattgcta cactctagag
     2221 ggaagaaaat ttatccacac caatctaagt gttgataaat tactgatgta ccagttaatg
     2281 aaattatctt tggaaaatcg cacaatagaa tacgcagaca atcgaatttc gctattttct
     2341 gctcaacatg gaaaatgtgc tataactctg gaagaatttc aacaagctaa tgaaatacat
     2401 tgtcatcata ttgtaccaac tggagctggt ggaaatgacg attataaaaa tcttattttg
     2461 gtaaaagagg cggtacaccg tctaatacac gctaaaacag aagaaacaat acaaaaatat
     2521 atagctctat tgaagctaaa taaagtccag ctcataagac taaataaata tagagtatta
     2581 gctggcaatc aagaattaaa cttgatatag gtctactaca taaatacttt tcaatataaa
     2641 cacgatggaa cgcggagtgc ggtgaaagtc gcacgctccg tgtgaatggg gggaaaagtt
     2701 tgagatattt ataatatc
//
LOCUS       DACGLW010000152         2693 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.161, whole genome shotgun sequence.
ACCESSION   DACGLW010000152 DACGLW010000000
VERSION     DACGLW010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2693
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.161"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            <1..1442
                     /locus_tag="IXT53_002870"
     CDS             <1..1442
                     /locus_tag="IXT53_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906628.1"
                     /translation="FLFLQSIYLSMPKLETFFTAQNTQAFSSRKYFLFFTVFLVLCWL
                     PYLLALYPGVVLPDSLSSVAQSMGDVPISNHHPVLFTLLVKLFVHDLPTDTINHGVFL
                     FSIFQTLITASAISYSLCWLLKRNIHFFPVLISLCYFAFAPVFPIYALNMQKDTLFSI
                     ACFLFSLAVFDFIFQREKPTILNQIYVIILALLTTYLRNNGIYVVTGTGLICGLIILK
                     QKRYQAFFQVVLGYTVIAGLLIHPLMARYAIPTETAEQLGVPLQQISRTIVMDGDLSK
                     KDKAFMNQLLPIKDYEAYSPSLADSIKWHKNFNNEFLNTHKKEFLSLWARNLPSNIKI
                     YTDAYILETFGFWVPGTKNSYGFLDTRVNTNNYGMKQVDLIEHLTGLSGMKKIIDARD
                     FFGSGTLLWLAIGSALLAIFKKKYRYLLLFLPGMLVFATIMIATPVAFSLRYVFVLAL
                     GLPVYILSPFLLDKREKMKTDIGDTHETI"
     gene            1429..2631
                     /locus_tag="IXT53_002871"
     CDS             1429..2631
                     /locus_tag="IXT53_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906629.1"
                     /translation="MKLFKKIFFAFVLLIASYCVLLTITFFIPQHSIESNAEKSLQMV
                     EKEGMYPSINQGNMLGTRLDNFTDHLMIRKTKADPELNSLENAMSMADYPRYWHGYQL
                     FLRPLLLGMSLGSIRMIYAAILFLLIGLTSYFLTKCSDIYLTTALLISLAIGNAATFF
                     FSMQFSNIWILTLLSVLLFLTKPQLSNTNQQLFLYFFVIGSLANFFDLLTTPVISWGI
                     PVIIIYYVTNKYLMDKRSSLSKQVGSFVFTGIFWGLGYGLTWVAKWILSSIILNKNIV
                     KDAINQILFRTEGNDKYPLHRLEMLKSNIRLMYPKVAILLLLITCLVFLYFAYRKRNS
                     WRPFQLLVLFVLAAVTPYIWYNILANHSQIHYWFTYRTQIITSFAILSAFAFLIPPEK
                     SKDELNYF"
BASE COUNT          771 a          481 c          466 g          975 t
ORIGIN      
        1 cttttttatt tttacaatcg atttatttat caatgccaaa actggaaaca ttttttactg
       61 ctcaaaacac acaggctttc tcttcaagaa aatatttttt attctttact gtctttttag
      121 ttctgtgttg gcttccttat ctgttggcat tgtatcctgg agtggttttg cctgattcgt
      181 taagttcggt cgcccaatct atgggtgatg ttcctataag caatcatcat ccagtcttat
      241 tcaccttact ggtcaagcta tttgttcatg atttaccaac tgatacgatc aatcacggtg
      301 tatttttatt ttcgattttt caaacattga taacagcttc agcgataagt tattctcttt
      361 gctggctgtt aaaaagaaac atccattttt ttccggtact cattagttta tgctattttg
      421 catttgcacc ggtttttcca atctacgcct tgaacatgca aaaagatact ttgttctcta
      481 tcgcttgctt tttattttcc ttagcggttt ttgactttat ttttcaacga gaaaagccaa
      541 cgattctcaa tcagatttac gtaatcatcc ttgctttact gaccacctat ctaagaaata
      601 atgggattta tgtagtaacc ggcacaggtt tgatttgcgg cttgattatt ttgaaacaaa
      661 aaaggtatca agcattcttt caagtggtgc tgggttatac agtgattgct ggactactca
      721 tccacccgtt gatggctcgt tatgctattc ctacagaaac tgctgaacaa ttaggtgttc
      781 ctttgcagca aatttctaga actattgtta tggatggtga cttgtctaag aaagataaag
      841 cattcatgaa tcagctactc ccgataaaag attatgaagc gtattctcct agtcttgcag
      901 actcaatcaa gtggcataaa aattttaaca atgagttttt gaatacacat aaaaaagaat
      961 ttctatcact atgggcaaga aatttaccaa gtaatataaa aatctataca gatgcttata
     1021 ttttagaaac gttcgggttc tgggtacctg gtacaaaaaa tagttacggc tttttagata
     1081 ctcgtgtaaa cacgaataat tatggtatga agcaagtaga tttgattgaa catcttactg
     1141 gattgtcagg aatgaaaaaa atcatcgatg ctcgtgattt ctttggttcc ggcacattac
     1201 tgtggttagc tatcggcagt gcacttttag caatctttaa gaaaaaatat cggtatttac
     1261 tcctttttct tcccggtatg cttgtttttg ctactattat gattgctact cctgtggctt
     1321 ttagtttacg gtatgtgttt gttttagcat taggtctacc tgtttatatc ctctctcctt
     1381 ttttactaga taaaagagaa aaaatgaaaa cagatatagg agatactcat gaaactattt
     1441 aaaaagatat tttttgcttt tgttctcttg atcgctagct attgcgtact actgacaatt
     1501 acttttttca ttccgcagca ctctattgaa tcgaatgcag aaaaatctct acagatggtt
     1561 gaaaaagaag gaatgtatcc ttctatcaat caaggaaata tgctaggcac tcggttagat
     1621 aattttactg atcatttgat gatacgaaaa acaaaagcag atcctgaatt gaactccttg
     1681 gagaacgcga tgtctatggc ggattatccc cgttattggc atggttatca gcttttttta
     1741 cgacctttac tgctagggat gagtttgggg agtattcgaa tgatttacgc agccattctt
     1801 tttctgctga ttggattgac gagctacttt ttaaccaagt gtagcgacat ctatttaacg
     1861 acagccttgt tgatttcgct tgctatagga aatgctgcaa cctttttctt cagtatgcaa
     1921 ttttcaaata tttggatttt gactttattg tctgtattac tatttttaac aaaaccacaa
     1981 ctctccaaca ccaaccaaca attattcttg tatttctttg tcattggttc tttagctaac
     2041 ttttttgact tgttgacaac accggtcatc tcttggggga tacctgtgat cattatttat
     2101 tatgtaacaa ataagtattt aatggataaa cgcagttcat tgagcaaaca agtaggcagt
     2161 tttgttttta ccggtatctt ttggggatta ggctatggtt tgacatgggt ggctaaatgg
     2221 atcctatcat cgatcatatt gaacaaaaac attgtaaaag atgcaatcaa ccaaatcctc
     2281 tttcgaacgg aaggaaacga caaatacccg ttgcatcgat tagagatgct gaaaagcaat
     2341 attagattga tgtatccaaa agtagcaata ttattgctat tgattacttg tcttgttttt
     2401 ctctattttg cttatcgtaa acgaaatagc tggcgtccgt tccaacttct agttttgttt
     2461 gttttagctg ctgtaacgcc gtatatttgg tacaatattt tagctaatca ctcacagatt
     2521 cattattggt ttacgtatcg tacgcaaatc atcacaagtt ttgctatact tagtgctttc
     2581 gcctttttga tccctcctga gaaatcaaaa gacgaactaa attactttta agaataccga
     2641 tgtactaatt catttttaaa cataaaaact ccagctgtca gagatagccg gag
//
LOCUS       DACGLW010000153         2398 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.162, whole genome shotgun sequence.
ACCESSION   DACGLW010000153 DACGLW010000000
VERSION     DACGLW010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.162"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            207..2069
                     /locus_tag="IXT53_002872"
     CDS             207..2069
                     /locus_tag="IXT53_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ4906630.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2398
                     /locus_tag="IXT53_002873"
     CDS             2069..>2398
                     /locus_tag="IXT53_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ4906631.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          865 a          353 c          466 g          714 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataat
//
LOCUS       DACGLW010000154         2270 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.163, whole genome shotgun sequence.
ACCESSION   DACGLW010000154 DACGLW010000000
VERSION     DACGLW010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.163"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            191..445
                     /locus_tag="IXT53_002874"
     CDS             191..445
                     /locus_tag="IXT53_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ4906632.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            446..763
                     /locus_tag="IXT53_002875"
     CDS             446..763
                     /locus_tag="IXT53_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ4906633.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            1322..2257
                     /locus_tag="IXT53_002876"
     CDS             1322..2257
                     /locus_tag="IXT53_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906634.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNMNITSNKTSRVLLALMG
                     YGILYRDDQNGQILINRAGRSLLMKYRKFIPEDFQNLQLENMRLEDLFRLAEDDKYRF
                     EVLKIQKNELLNKLYPENAIKAHFGDANHMYSWFIPKRFRGKINAGDQVKVLNRNSTD
                     VVMITDIYVEDEDIIKYMKPVIKLVNKNKGTRGAS"
BASE COUNT          823 a          307 c          429 g          711 t
ORIGIN      
        1 gatgttaacg gaagtgtatg gtgagtagtt cactcgtatt ttgatatcgt ttgtatatgt
       61 tttcagcgtt ctaagtggct tattttgtcc aataataata tggagtggaa tcccttaaga
      121 aacaccgttg gcaattgttg ttataacatg ttataattta ttcatcaaaa actaaaagga
      181 gtgtgataac atgggaaaag aactaaaggt tagaaagatc ggcaatagcg tgggagtgat
      241 ccttccaagc tctcttggat taaaaagcgg agacattatc caagccaaac aagaaggcaa
      301 cttattcata ttagatacta ctcaaatagc aaaagaacac gatcgcaaat tgattgaaga
      361 aagtttccaa gactttgaga aaggtttaac agtttcagaa attgaaatgg taaaagcttt
      421 cggaaaatac ggctggagtg aatagatgaa agagtacacg ctagtttatt ctaaaagttt
      481 tcagaccagc cttgaaacga taatagaaga atgggaaaat gagcttgctc tttctccagc
      541 cagcataaaa agatttgtag gtgcgatcca gaaggcttta gaactaacca aaaattttcc
      601 aactatgcat gaggaagttt ccgaaatcta tggaatggat atcccaacat atcggatcgt
      661 gatcggcaaa agctatgcct tattttatcg gatcgaccat gaaaaaaaag agatacttgt
      721 aggacgtatt ttccaatcta aacaaatgaa actaggcttt taacaataat taggaaaaag
      781 tcgctcatcc tctacaggtg ggtgattaat gccgctcgat atggttgtta accgtcggtt
      841 actcgaagat tgatatataa gacttagtac ctttttattt tgctccatgt tatggccgaa
      901 ggtctaaaga tacaatcatt agtctagaga aaaattaaga accgtaggaa ctacggggat
      961 cgcttggtga ataagagaca ctgctattag tgaaaaaata agatatcaag tacgctctat
     1021 tcccaaaaat ttcgtgactt tactcatgag aggttcaagt agtatatttt taatcaaaat
     1081 tgacgtaact tttaataaat actacctaaa taaggtagta tttatttttg tgttttaaaa
     1141 gaggaagtcc aatatatcta tgggaaactt cttaaatatt atgttggtac atgaaaatat
     1201 acatccctgc tagactattt acaacatttt actttgtacg tattcttgtt ttaaaaggaa
     1261 tgggataaaa tataggtaca ataactattt gatgctaaat ataattaatg gaggagagat
     1321 agtgaacata cttaatgaaa aaagatatgt atggtttgct ggtaaaaaat atattactac
     1381 tttcaaaaga ttatccgagt taatgcagca gactgttaaa agtgctatgc ctgaaggata
     1441 caagtttaat tttgttcaat cttcagtatc tactgaaagt atctattttt caattctttc
     1501 tgagagtagg ctttttcatt ttagaataag ttctcatgta aagaaaactg atgttgatat
     1561 atatacgttc cgtacaaata attataaaaa tttaaatgat ttgagtagaa gtatcagtga
     1621 atatctcaag gggaatagct atctcaagat tcgattttca catatattaa ttttactacc
     1681 atatttgaat gagaatttca ccctaattca agtagataag tctttcattg cctcaggtaa
     1741 tcaaggaaat atgaatatta caagtaacaa aacgtcacgt gtgctattgg cgttgatggg
     1801 ttacggaatt ctttatagag atgaccaaaa tggccagatt ctaataaatc gggctgggcg
     1861 ttcattatta atgaaatata gaaaatttat accggaagat tttcaaaact tacagttaga
     1921 aaacatgcgt ttagaagatt tatttagatt agcagaagat gataagtata gatttgaagt
     1981 tttgaaaatt caaaagaatg aactattgaa taagttatat cctgagaatg cgataaaagc
     2041 acattttggt gatgctaatc atatgtatag ttggttcatt cctaaaagat ttagagggaa
     2101 aatcaatgct ggagatcaag tgaaagtact caatcgaaat agtacagatg tcgtaatgat
     2161 aacagatatc tatgtagaag atgaagatat aatcaaatat atgaagcctg tgataaaact
     2221 tgttaataaa aataaaggaa cccgtggtgc tagttaattt tttcctagag
//
LOCUS       DACGLW010000155         2123 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.164, whole genome shotgun sequence.
ACCESSION   DACGLW010000155 DACGLW010000000
VERSION     DACGLW010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.164"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            complement(505..578)
                     /locus_tag="IXT53_002877"
     tRNA            complement(505..578)
                     /locus_tag="IXT53_002877"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(542..544),aa:Ile,seq:cat)
     gene            complement(767..1786)
                     /locus_tag="IXT53_002878"
     CDS             complement(767..1786)
                     /locus_tag="IXT53_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HAQ4906635.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RSDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            complement(1797..2003)
                     /locus_tag="IXT53_002879"
     CDS             complement(1797..2003)
                     /locus_tag="IXT53_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HAQ4906636.1"
                     /translation="MNNRTFDILKWIAIVVIPALATFVGLVGKGVNWRYTDITVMIIT
                     GFGAFLGSILGVSNRTYKKISSDK"
BASE COUNT          638 a          421 c          352 g          712 t
ORIGIN      
        1 acctaaaaag gtagcccgga acgaatttta tcaccatact tgtgaaagga gatatttttt
       61 aagttagtat taagattgtg taatatgatg atatctatat tttatagtat cagtgctata
      121 aaatcaaaaa taagtcacta attaactacc actccaattg taagcctttt ttctcacttt
      181 ttttcaaaaa tatggtatgc cttttaatag cttcaaatat aaaagagttt aaagcgtaac
      241 acacttatgg ggaagtggct tggggtgcgc tttaaactct tctttattat catctcacaa
      301 tttaacccaa atgtctttct atttaaaaat caaagtaaaa cttttcaaat atacagaaat
      361 ataactatgt gaaacatcct ttcattaatc cataaaagga tacataaaaa agccactcat
      421 ttgagtggca atgaagaaaa gctttagctt gtataatact cttcaaaaaa ttctaacaca
      481 gaacgattca aatggctacg ttaatgtacc ctgtaggact cgaacctacg accggacggt
      541 tatgagccgt ctgctctgac caactgagct aagggtactg gttgttgcca cataaagcca
      601 taaacaatca accagtagaa tgtgtggcaa caaacctgtt atcgcatagc ttggagtgtg
      661 actattatgg gtgatagtga agatatgcga taacatcact attttatcga atgattttta
      721 tagttgtcaa tatagttatg taatgctcct caacgaggag ctatttttat cgtttaggaa
      781 tatttaaata ccaacgtttg tcatggaaat cttgcgcacc gcctttagtg tttccctctg
      841 gatcattcgt tgcccgcatc atgacataga ctttcttatt agggaagtta cgcatgttaa
      901 aagatacatg atagccaaca tttccagaag tattataagc ttgatttaca tctgatctat
      961 aaattccatc agctcttact cgagctaatt ctttcccagt attgtaatcc ataatgaaga
     1021 tatactcgta tttatagtta gcaatgtgcc atccagctac atgcaaattt gcattttcga
     1081 tttctccaaa ctgatcaatg tgggcgtaat tcgttccatc tgtcagtgta ggattagctg
     1141 caccagctcg tgttggatca atgacaggct tgttttcaga agttgttgga ttttcatcgg
     1201 taaatccatg agctaaatca tatgctaatt tttctttact tacgcccatt tcagaaagat
     1261 aaccgtaagg atctgtatga tcgccccaga tgttttgtgt tacccataaa tgcgatttga
     1321 ttcccggttg gttataagga gtgtccaacg ttaatggaat accatatttc attgctgaat
     1381 ctctagccaa ttcaacgtat gccttgtagt ttttctcaaa cgttgcttta tcatgtgtgt
     1441 gttgtaactc aatctgcaca ggactgttgg cattagcata cgaaccagca ccgtactgta
     1501 cataaccagg ttgaccgact tgataaacaa ttccgccgtc tcccacaata taagcagtat
     1561 aagcgctagt ccatgaacgt tgcatatact gcgcttcatt gcgtcctgtt gctgtttcat
     1621 tagccgtttc atgcagtaaa atatactgat tatttgctac ttgtgagcta ccttcatttg
     1681 cgcccaaatt aaattcattg ttgatagtat aggcaaatcc gttaattggc aataaaaaaa
     1741 gagccgttaa caggctcatc gcagtaatag taattttctt tttcatatct atcctcctat
     1801 ttatctgatg aaattttctt atacgttcga tttgatacac ctaaaatact ccctagaaat
     1861 gctccaaaac ctgtaataat catcaccgtt atatctgtgt accgccagtt aacacctttt
     1921 ccgacaagac cgacaaaagt cgctaaagcg gggataacta ctatagcgat ccatttcaaa
     1981 atatcaaagg ttctattgtt catactatcc acctcccttc attggttggg ctactttctt
     2041 tttcaatcgc ttgttttctt ctatcaatat ttctctttct gaaagcaagg catcaatttg
     2101 cccgtccagt tgttcaattt tat
//
LOCUS       DACGLW010000156         2003 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.165, whole genome shotgun sequence.
ACCESSION   DACGLW010000156 DACGLW010000000
VERSION     DACGLW010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.165"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            <1..378
                     /locus_tag="IXT53_002880"
     CDS             <1..378
                     /locus_tag="IXT53_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HAQ4906637.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /gene="yfcC"
                     /locus_tag="IXT53_002881"
     CDS             426..1826
                     /gene="yfcC"
                     /locus_tag="IXT53_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HAQ4906638.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            1859..>2003
                     /locus_tag="IXT53_002882"
     CDS             1859..>2003
                     /locus_tag="IXT53_002882"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HAQ4906639.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAF"
BASE COUNT          555 a          339 c          432 g          677 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt tta
//
LOCUS       DACGLW010000157         1954 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.166, whole genome shotgun sequence.
ACCESSION   DACGLW010000157 DACGLW010000000
VERSION     DACGLW010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1954
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.166"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            156..1862
                     /locus_tag="IXT53_002883"
     CDS             156..1862
                     /locus_tag="IXT53_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin/fibrinogen-binding protein"
                     /protein_id="HAQ4906640.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
BASE COUNT          699 a          387 c          353 g          515 t
ORIGIN      
        1 acctaaaaat ttggatttca acgatagaaa cactgttttt tatgtaatta gtccaagatt
       61 tttctagact tttgctttac tattcggcca aattggtttt tcttcttttt ttcgttataa
      121 tgaatatact tatgaaaaga aatgaggaat gaaccatgtc ctttgatggt gtatttaccc
      181 acgcgatggt caacgaattg cgtgaaacgt tactatctgg ccggatctcg aaaatccatc
      241 agccttatga aaatgaaatc gtgctggtga ttcgttcacg cggcaaaaac catcgattat
      301 tattatctgc gcacccaagt tatgctcgtg tacagattac ccaaatcgat tatcagaatc
      361 ctgataatcc gcctaacttc gtgatgatgc ttcgaaaata tttggatggc gctattttag
      421 aagacatcga acaaatcgaa aatgatcgag tgatccattt ccactttgct aaacgaaacg
      481 agttgggtga tctacaaaat atcatattga tcgttgaatt aatgggacgt catagtacga
      541 tcgtcttagt taatcgagaa actggaaaaa tactggatgc gatcaaacac attggaagct
      601 ctcaaaacac ttatcgttct ttgcttcctg gtgtagaata tgttgctccg ccaaaacaag
      661 aggtcttgaa cccatttagc tccgagaaag aaaaaatctt tcagcggctt tcccaaactg
      721 aactagatcc aaaagcaatc caacgtcagt ttcagggaat cggtttcgat atcgctcaag
      781 agctgaccaa aagattactg gaacgtccta atgaaaagat ggttgtatgg gacgaatttt
      841 tctcagccat cagccatcag cctattccta ctttttacga aacggaaaat aaagattttt
      901 tcacaccgat tgcttatcag gtattgtccg aacaggcatc tgctgtaaca gcttatccaa
      961 cactcagtca attattagat agctattatc acgaaaaagc agagaaagat cgggcaaaac
     1021 aacaaggcgg agaactgatt cgaaaaatcg aaaatgaact aaaacgaaat aaaaataaat
     1081 tgaaaaaacg tgaacaaaca ctcaaagaat ccgaaaacgc tgagaattac cgtagaaatg
     1141 gcgaactact tactactttt ctgacacaag taccacgcgg cgcaaaagaa gttgttttac
     1201 ctaattatta tgaagaagat cggccaatca aaatagcact agatccggca ttgacaccaa
     1261 atcaaaatgc acagaaatat ttccatcgat accaaaagct gaagaacgct gttaagctga
     1321 ttggcgaaca aatccaagaa gccaaagatg aaatccaata tcttgaatct gtcttatctc
     1381 aactggaaat tgccggccca atggatatcg aagcaattaa agaagaattg acttcagaag
     1441 gatacctgaa aaagaagaac ttaaaaaaac aaaaacgtaa gaaaccatct cagcctgatc
     1501 aatacttttc ttctgacgga acgctcattc ttgttggtaa aaacaatctt caaaatgacc
     1561 agctgacaat gaagacagct aaaaaaaccg actattggct tcatgcaaaa aatatacccg
     1621 gttcgcacgt catcattaaa agtgacaagc cctcagatga aacaattaca gaagctgccg
     1681 agttagctgc ctatttttca aaataccgat actctgccca agtgccggtt gatcttgtac
     1741 aggtaaagca tatccgaaaa ccaaatggcg ccaagcctgg ttatgttatt tatgaaaacc
     1801 aaaagacgat tatcgtgaca ccagaagaag aaaaaattac agctatgaaa caaaacaggt
     1861 aatcaatgag tgtttctaga atcatatttg cgtactttcc cacaacagca attagttata
     1921 aattgtttgc tacaaaaaga ggagttgtga caaa
//
LOCUS       DACGLW010000158         1719 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.168, whole genome shotgun sequence.
ACCESSION   DACGLW010000158 DACGLW010000000
VERSION     DACGLW010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.168"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            10..375
                     /locus_tag="IXT53_002884"
     CDS             10..375
                     /locus_tag="IXT53_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ4906641.1"
                     /translation="MVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCYTLLRPDGKT
                     MFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRACHVYEKLGG
                     KNDYKDEIVYVYDYEKGDK"
     gene            376..>1719
                     /gene="aph(2'')-I"
                     /locus_tag="IXT53_002885"
     CDS             376..>1719
                     /gene="aph(2'')-I"
                     /locus_tag="IXT53_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(2'')-Ia/If/Ih family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HAQ4906642.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYDGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKE
                     IKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYIL
                     LRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGII
                     DFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYY"
BASE COUNT          711 a          130 c          296 g          582 t
ORIGIN      
        1 gatgataata tggtttttga tattgataat ttaaaaggat ttcttaatga taccagttca
       61 tttgggttta tagctaaaga aaataataaa attataggat ttgcatattg ctatacactt
      121 ttaagacctg atggaaaaac aatgttttat ttacactcaa taggaatgtt acctaactat
      181 caagacaaag gttatggttc aaaattatta tcttttatta aggaatattc taaagagatt
      241 ggttgttctg aaatgttttt aataactgat aaaggtaatc ctagagcttg ccatgtatat
      301 gaaaaattag gtggtaaaaa tgattataaa gatgaaatag tatatgtata tgattatgaa
      361 aaaggtgata aataaatgaa tatagttgaa aatgaaatat gtataagaac tttaatagat
      421 gatgattttc ctttgatgtt aaaatggtta actgatgaaa gagtattaga attttatgat
      481 ggtagagata aaaaatatac attagaatca ttaaaaaaac attatacaga gccttgggaa
      541 gatgaagttt ttagagtaat tattgaatat aacaatgttc ctattggata tggacaaata
      601 tataaaatgt atgatgagtt atatactgat tatcattatc caaaaactga tgagatagtc
      661 tatggtatgg atcaatttat aggagagcca aattattgga gtaaaggaat tggtacaaga
      721 tatattaaat tgatttttga atttttgaaa aaagaaagaa atgctaatgc agttatttta
      781 gaccctcata aaaataatcc aagagcaata agggcatacc aaaaatctgg ttttagaatt
      841 attgaagatt tgccagaaca tgaattacac gagggcaaaa aagaagattg ttatttaatg
      901 gaatatagat atgatgataa tgccacaaat gttaaggcaa tgaaatattt aattgagcat
      961 tactttgata atttcaaagt agatagtatt gaaataatcg gtagtggtta tgatagtgtg
     1021 gcatatttag ttaataatga atacattttt aaaacaaaat ttagtactaa taagaaaaaa
     1081 ggttatgcaa aagaaaaagc aatatataat tttttaaata caaatttaga aactaatgta
     1141 aaaattccta atattgaata ttcgtatatt agtgatgaat tatctatact aggttataaa
     1201 gaaattaaag gaactttttt aacaccagaa atttattcta ctatgtcaga agaagaacaa
     1261 aatttgttaa aacgagatat tgccagtttt ttaagacaaa tgcacggttt agattataca
     1321 gatattagtg aatgtactat tgataataaa caaaatgtat tagaagagta tatattgttg
     1381 cgtgaaacta tttataatga tttaactgat atagaaaaag attatataga aagttttatg
     1441 gaaagactaa atgcaacaac agtttttgag ggtaaaaagt gtttatgcca taatgatttt
     1501 agttgtaatc atctattgtt agatggcaat aatagattaa ctggaataat tgattttgga
     1561 gattctggaa ttatagatga atattgtgat tttatatact tacttgaaga tagtgaagaa
     1621 gaaataggaa caaattttgg agaagatata ttaagaatgt atggaaatat agatattgag
     1681 aaagcaaaag aatatcaaga tatagttgaa gaatattat
//
LOCUS       DACGLW010000159         1710 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.169, whole genome shotgun sequence.
ACCESSION   DACGLW010000159 DACGLW010000000
VERSION     DACGLW010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.169"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            <1..537
                     /locus_tag="IXT53_002886"
     CDS             <1..537
                     /locus_tag="IXT53_002886"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906643.1"
                     /translation="VMAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNG
                     QPMNDKRNGHSWFRQQERIENKVHSLREEIKQQEKQVEKLERQEEIKEMGYNKYGGLD
                     MTIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQA
                     IIDSGRVTQWKKIRPFIF"
     gene            564..>1710
                     /locus_tag="IXT53_002887"
     CDS             564..>1710
                     /locus_tag="IXT53_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906644.1"
                     /translation="MNENGEFEESKKYQATSEEEKAAVRELLEEAVPTDFLKEKENDH
                     FYRVEFNENDPEKGLKSYGGEIVTFELIDQLKTLDNQYAAEAGYYKFYFEEVKNGEVV
                     DQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVSKEQMQSKETKTKQKG
                     YQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFPERSFRNQLLIEEQ
                     FPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGFVREIEATPEDKKR
                     IESGQIEVIKKTYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQLDKKGQSELQAGIQ
                     AVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNTRNT"
BASE COUNT          698 a          273 c          376 g          363 t
ORIGIN      
        1 gtcatggcag aagaaaaaga accactagac aatagtcgtt tgggaaaatc caaaagaaaa
       61 ttagtacgat tgcaaaatga attaaacgaa caaattgaaa agatgtttga acaccagaga
      121 aagaccaacg gtcaaccaat gaacgataag agaaatgggc attcttggtt taggcaacaa
      181 gagcgaatcg aaaataaagt gcatagtctg agagaagaga tcaagcaaca agaaaaacaa
      241 gtagaaaaac ttgaaagaca agaagagata aaggaaatgg gctataacaa gtacggcgga
      301 ctggatatga cgatcgagaa tattccaaga atcaaagaag aaatcgaacg ctttgaaaaa
      361 ggtgaatcga ctttttcagc cgctacgatc cgaaaatacc aacggaaatt ggaaacatta
      421 gaacagctga aagaaagatc ggaaaaagga aaagaaaacc ttcttcctga agtacaagca
      481 atcattgaca gcggacgagt gacgcaatgg aaaaaaatcc gaccatttat tttctgaaag
      541 gctatcgaaa agtcgcttta gaactgaatg aaaacgggga atttgaggaa tctaaaaaat
      601 accaagccac gtcggaagag gaaaaagcag ctgttcgtga attacttgaa gaggctgtac
      661 caactgattt tttgaaggag aaagaaaacg atcattttta tcgagtagaa ttcaacgaaa
      721 atgatccaga aaaaggattg aaaagttatg gtggggagat cgtaacattc gaattgatcg
      781 atcaactcaa aacgttggat aaccaatacg ccgcagaagc aggttattat aaattctatt
      841 ttgaagaagt gaagaacgga gaagtagtcg atcagatccg aatggatcta ggggatggcg
      901 tcaacagtaa tcaacccatc tatttagagt tagcaaaatt gtgcgaacaa gtacctgaga
      961 aggaacaaaa acaatcggtc aaaaatgaac ctgtttcgaa agaacaaatg cagtcgaaag
     1021 aaacaaaaac aaaacaaaag ggctatcaaa aacgaacggc agaagagatt aaacaagaag
     1081 ttaaatccct ttcgcaacag gcactggatg cggtgaaaaa gcatactcat tctcctgaag
     1141 acgtcaaaga acttttgaac tttatgtctc gttttccaga acgatccttt agaaatcaat
     1201 tattgattga ggaacaattt ccaggtgcaa cggcttgttt aggtcgtgcc gcgttgaaaa
     1261 aagaaggaat ctatatcaaa aaaggggaac aagggaacaa gatctttgtc agaaaaacgg
     1321 tcaaaggatt ttatgagaaa ggacgtggct ttgttcgaga aatagaagct acaccagaag
     1381 ataaaaaacg aatcgaaagt ggtcaaatcg aagtgatcaa aaaaacttac tacacgattg
     1441 aaaaagtgtt tgatatcaca caaacacagt taaaacctga agactatccg aaaatctttc
     1501 cgaaccgtgt ctttgatttt caattggata aaaaaggaca gtcagaactt caagctggga
     1561 ttcaagcggt agccaacaat attggaatac aaattcgaga tatgacagaa agtgaagtct
     1621 atcaaagaga attaggacaa gcacgtgggg cgtatgttcg taacgaattt actggcgaag
     1681 aagaaatcgt catgaatact cgaaatactc
//
LOCUS       DACGLW010000160         1642 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.170, whole genome shotgun sequence.
ACCESSION   DACGLW010000160 DACGLW010000000
VERSION     DACGLW010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1642
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.170"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            complement(<1..209)
                     /locus_tag="IXT53_002888"
     CDS             complement(<1..209)
                     /locus_tag="IXT53_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ4906645.1"
                     /translation="MDTITPTNFKENIGKVLQEVVHSNSEIEITRDSKNGLNDGVIMI
                     SKRKWELLQEELYLQRTGTWAYVSD"
     gene            complement(308..622)
                     /locus_tag="IXT53_002889"
     CDS             complement(308..622)
                     /locus_tag="IXT53_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906646.1"
                     /translation="MAEENKVVPFETLVINKEEFEQLFLDFFIQYDMIYDFSSSLDLF
                     ELEAEIIENSREKNGKIIISVEKFNELFSQTFEIDPDYYQPQEVFREFRNKIIGFSQT
                     TK"
     gene            complement(690..1184)
                     /locus_tag="IXT53_002890"
     CDS             complement(690..1184)
                     /locus_tag="IXT53_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906647.1"
                     /translation="MFKVYFPFQWLVKGILLVLLGLILFGSYLTIKARHFPEEKPIKI
                     VTKDLKIGETYQADGVELTFKNIEVTEKRNEFAQHDRVLQVTYTLINDTENPIRPQQW
                     SIENKDGEELYEYDMILDNPEYIESGKEETVNYYYELPIERQDYQIVVRRENGYIVGL
                     PFTL"
     gene            complement(1177..1491)
                     /locus_tag="IXT53_002891"
     CDS             complement(1177..1491)
                     /locus_tag="IXT53_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906648.1"
                     /translation="MNYPYFKVSASEETKEIFNNFYNQNKGIFGSKANMFRVMVSNLP
                     VLASPSNNKFNDPESIKFEQKISELESMISNEVIEKLDDIDQKLSYSLKNKYKTEEKK
                     DV"
BASE COUNT          494 a          306 c          220 g          622 t
ORIGIN      
        1 aaatcagata cataagccca tgtacccgtt cgttgtaaat aaagttcttc ttgtaacaac
       61 tcccattttc tcttagaaat cataatgacg ccatcgttca atccattttt agaatcccta
      121 gtaatttcta tttcagaatt tgaatgaact acttcttgca acactttacc tatattttct
      181 ttgaagtttg ttggagtaat agtatccaaa atgaatacct ctcttctcta tacctctatt
      241 atgaagtgcc cataaaataa agcaagcgtt gaggaataat tctatcaaca cttgctttat
      301 taagctttca ttttgtagtt tgactaaaac caataatctt atttctaaac tctcgaaaaa
      361 cttcttgtgg ttgataatag tctggatcta tttcaaatgt ttgtgagaat aattcattga
      421 atttttctac tgaaataatg attttaccat tcttctctcg actgttttct attatttctg
      481 cttctaattc aaataaatct agtgacgaac taaaatcata aatcatgtca tattgaataa
      541 aaaaatcaag aaaaagttgc tcaaactctt ccttatttat cactaatgtt tcaaacggaa
      601 caactttatt ttcctcagcc attctcccag ttccattttc aaaaatcaca aaactgatat
      661 taccttaaaa actactaaca aaataaaact tataaagtaa atggcaaccc aactatgtaa
      721 ccattctctc tacgtactac gatttggtag tcctgccttt caatcggtaa ttcataatag
      781 taatttactg tttcttcttt gccactttca atatactctg gattatccaa tatcatatca
      841 tactcataaa gttcttcacc atccttattt tcaatcgacc attgttgtgg acgtatcgga
      901 ttttctgtat catttataag tgtataggtc acttgtaata cacgatcatg ttgtgcaaat
      961 tcatttcttt tttcggtcac ttcaatattt ttaaatgtta gttcaacccc atctgcctga
     1021 taagtttcac ctatttttaa atcttttgtt acaattttta ttggcttttc ttctggaaaa
     1081 tgtctggctt taattgttag ataacttcca aaaagaatca atcctagtag cactaaaaga
     1141 attcctttaa cgagccactg aaacgggaag taaactttaa acatcttttt tctcctccgt
     1201 tttgtatttg ttttttagag aatacgataa cttttgatca atgtcatcca gcttttcaat
     1261 tacttcatta cttatcattg attctagttc tgaaattttt tgttcaaatt taatgctttc
     1321 aggatcatta aatttattat ttgatggact agctaacact ggtaaatttg aaaccataac
     1381 acgaaacata tttgcctttg aaccaaaaat tcctttgttt tggttgtaaa aattgttgaa
     1441 tatttcttta gtttcttctg aagccgagac tttaaaatat ggatagttca tttttttctt
     1501 ccacctttct ttttttggga gggagtctgt agggagcttg ctccctcaga gatttacccg
     1561 ccatctgact tgccagaaag tagcctagcg tgagcggggc ggattgactg gcaagtagat
     1621 gggtagctgg cgtatgtcct aa
//
LOCUS       DACGLW010000161         1572 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.171, whole genome shotgun sequence.
ACCESSION   DACGLW010000161 DACGLW010000000
VERSION     DACGLW010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1572
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.171"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            46..105
                     /gene="ermCL"
                     /locus_tag="IXT53_002892"
     CDS             46..105
                     /gene="ermCL"
                     /locus_tag="IXT53_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002497446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methylase leader peptide ErmCL"
                     /protein_id="HAQ4906649.1"
                     /translation="MGIFSIFVINTVHYQPNKK"
     gene            165..899
                     /locus_tag="IXT53_002893"
     CDS             165..899
                     /locus_tag="IXT53_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011282421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(T)"
                     /protein_id="HAQ4906650.1"
                     /translation="MNKKNIKDSQNFITSKHHINEILRNVHLNTNDNIIEIGSGKGHF
                     TFELAKRCNYVTAIEIDPKLCRITKNKLIEYENFQVINKDILQFKFPKNKSYKIYGNI
                     PYNISTDIIRKIVFESTATESYLIVEYGFAKRLLNTNRSLALFLMTEVDISILSKIPR
                     EYFHPKPRVNSSLIVLKRHPSKISLKDRKQYENFVMKWVNKEYIKLFSKNQFYQALKY
                     ARIDDLNNISFEQFLSLFNSYKLFNR"
BASE COUNT          588 a          248 c          211 g          525 t
ORIGIN      
        1 cttgtgctat aattattcta atttcataaa aaggagaatt aatatatggg catttttagt
       61 atttttgtaa tcaacacagt tcattatcaa ccaaacaaaa aataagtggt tataatggat
      121 tgttaatata aacattcatt ataacctcat aggagtgggt tataatgaac aaaaaaaata
      181 taaaagatag tcaaaacttt attacttcaa agcatcatat aaatgaaatt ttgagaaatg
      241 tacatttaaa tacaaatgat aatattattg agattggttc agggaaaggt catttcacgt
      301 ttgaattagc taaaaggtgt aattatgtaa ccgccattga aatagatcct aaattatgta
      361 ggataactaa aaacaaactt attgaatatg agaacttcca ggttatcaat aaagatattt
      421 tacaatttaa gtttcctaaa aataagtcat ataagatata tggaaatata ccctacaata
      481 taagtacaga tataattcga aaaattgttt ttgaaagcac agctacagaa agttatttaa
      541 tagtggaata tggatttgct aaaaggttgc taaatacaaa tcgttcacta gcactatttt
      601 taatgacaga agttgatata tccatattaa gtaaaatccc tagagaatac tttcatccaa
      661 aacctagagt taatagctcg ttaattgtat taaaaagaca cccttcaaaa atatcactca
      721 aagatagaaa acaatatgaa aattttgtta tgaaatgggt taacaaagaa tacataaaac
      781 tattttccaa aaaccaattt tatcaagcct taaaatatgc aagaattgac gatttaaaca
      841 atattagctt tgaacaattc ttgtctcttt tcaatagcta taaattattt aatagataag
      901 ttaaggggtg cataaactgc atcccttaac ttatcttatt ttgcttaaac aatcttggcc
      961 cgtaaactgc ataactcata tgtaaatcgc tcctttttag gtggcacaaa tgtgaggcat
     1021 tttcactatt tccggcaatt acttccatgt aaagtataac ccactatact ttgcaagcaa
     1081 agtgtgggct cgccgagggg tcttgcagac ggctctttcg gctcttttga gccgagtatc
     1141 agcgcaccct tatggtgcaa ttagctgata cctgtttgac gatttgctgc cctacctttt
     1201 cggtaggtat gcaaacctca aaccaaaata aaaaccccct tcgacttcgt cagggggttt
     1261 ttatttatat aaatctccgt aaataatcgc gatttaataa catcttcaat tttatttttt
     1321 tgcgtatttt agaaatgttt tcactaaaaa atatattata caaaaaatac agtaatcgat
     1381 tataattctt tgccagttaa atatcatatc gttattaaaa tatcggaaaa aaatatcaac
     1441 aacaataaat gcaaaagaag accataaaaa ttgtttatta ctcatacata ctctccattt
     1501 ctatcatttt taaatttacc agtcatctct caaaaatctc ctagctattt ttctttttta
     1561 accacatacc gt
//
LOCUS       DACGLW010000162         1569 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.172, whole genome shotgun sequence.
ACCESSION   DACGLW010000162 DACGLW010000000
VERSION     DACGLW010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.172"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            24..635
                     /gene="mobC"
                     /locus_tag="IXT53_002894"
     CDS             24..635
                     /gene="mobC"
                     /locus_tag="IXT53_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HAQ4906651.1"
                     /translation="MDDKQKMYEKNRKHSEALNFRVDKKTYLELTLIAKQRGISRADV
                     CRERLKGLKYEPPLLAEDDRVVLTKQLNQIGNNLNQAVKALNIIAKYFREESEDKKEK
                     SLHFSFDASRYNIFKDELANSIAKKLMISYPDDIFLESNRSAPSFFKLSEVEKEYFEY
                     WIEKQTRPGTKEEYSQKVDRNLIHSIQIIASLKDEVKQLWELL"
     gene            620..>1569
                     /locus_tag="IXT53_002895"
     CDS             620..>1569
                     /locus_tag="IXT53_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HAQ4906652.1"
                     /translation="MGATITSKGRKKNLSSLLQYAANPSKKEKCVAVSGINCSDDSEE
                     ALQEMNLVRTKMNKSKDIAQAFHVIHSFDKSTSKELSIDKMHEIAVEFAEKAFPNSQI
                     IVASHNDKDHFHSHLVINNINMETCKRLRIDPKDLELMRKINDQVLNEHGLESVNNEY
                     YENLEKRTNLYSNDPSKMKYGKINYQEFVQNAIEATLEKCEVNSFEDFSDHLAKEYNI
                     EVYQFSKTNNKLGYVLYKQGVNFYERRGELITLGRDKQSRKEKSQYVERTFSARKLGK
                     RYSFESVSEQLEKNLEESHIKNFFNMEREQVMSLISGVNY"
BASE COUNT          622 a          203 c          296 g          448 t
ORIGIN      
        1 aaaaaaatgt gaggggataa tcaatggatg ataaacaaaa aatgtacgaa aaaaatcgca
       61 aacatagtga ggccttaaat tttcgtgtcg ataaaaagac ttatcttgaa ctgacattga
      121 ttgcaaaaca aaggggaatt tctagagcag acgtttgccg tgagcggtta aagggactca
      181 aatatgaacc gccgttgtta gctgaagatg atagggtagt tttaaccaaa cagttaaatc
      241 aaattggtaa caatttgaat caagcggtaa aggcattaaa tatcattgct aaatatttta
      301 gagaagaatc tgaagataaa aaagaaaaga gcctacattt tagttttgat gctagccgtt
      361 acaacatttt taaagatgaa cttgcgaata gcatagcaaa aaagttgatg attagttatc
      421 cagatgacat ttttttggag tccaatcgga gtgccccctc tttttttaaa ttatcagaag
      481 tagaaaaaga atatttcgaa tattggatag aaaagcaaac tagaccgggt acgaaagaag
      541 agtattccca aaaagttgat cggaatttaa ttcactcaat tcagattatt gcaagtttaa
      601 aagatgaggt gaaacaactt tgggagctac tataacatcg aaaggacgca agaaaaattt
      661 atcctcgcta ttacaatatg cagctaatcc ttcaaaaaaa gaaaaatgtg ttgcagtctc
      721 aggaataaat tgtagtgatg atagtgagga agcactgcaa gaaatgaatt tagtacgtac
      781 caagatgaat aaatctaaag atatagcaca agcgtttcat gttattcatt cgtttgataa
      841 gagtacttct aaagagttaa gtattgataa aatgcacgaa atagctgtcg aatttgcaga
      901 gaaagctttt ccaaattcgc aaatcattgt tgcaagtcac aatgataaag atcattttca
      961 tagtcatctt gtcattaata atattaacat ggaaacatgt aaaaggttgc gtattgaccc
     1021 taaagattta gaactaatgc gaaaaattaa cgatcaagtc ctcaatgaac atggtcttga
     1081 atcagtgaat aatgagtact atgaaaactt agaaaagaga accaatcttt attcaaacga
     1141 tccttctaag atgaaatatg gaaaaattaa ttatcaagag tttgtgcaaa atgcgatcga
     1201 agcaacgttg gaaaaatgcg aagtgaattc ttttgaagat ttttcggatc atttggcaaa
     1261 agaatataac attgaagttt atcaattttc aaaaacaaat aacaaacttg gctatgttct
     1321 ttacaaacaa ggtgtaaatt tctatgagcg tagaggtgag ttaattactt taggaagaga
     1381 taaacaatct agaaaagaaa aaagtcagta tgtcgaacgt acttttagtg caagaaagtt
     1441 agggaaaaga tattcatttg aatctgtttc tgaacaactt gaaaagaatt tagaagaatc
     1501 acatataaaa aattttttta atatggaaag agagcaagtt atgtcgctaa ttagtggagt
     1561 aaattacaa
//
LOCUS       DACGLW010000163         1515 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.174, whole genome shotgun sequence.
ACCESSION   DACGLW010000163 DACGLW010000000
VERSION     DACGLW010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.174"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            287..694
                     /locus_tag="IXT53_002896"
     CDS             287..694
                     /locus_tag="IXT53_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906653.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVNLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            708..1109
                     /locus_tag="IXT53_002897"
     CDS             708..1109
                     /locus_tag="IXT53_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906654.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1111..1482
                     /locus_tag="IXT53_002898"
     CDS             1111..1482
                     /locus_tag="IXT53_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906655.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
BASE COUNT          576 a          185 c          263 g          491 t
ORIGIN      
        1 tgaggggaag tacatatagt tgttgatata gttgctaata ttgttagcta atttgttagt
       61 aaaactgtat acgaatttag atagtaaaaa aacagtttgt tccttatata gaggcacttt
      121 tactactgat taaatgtcag atatgcttac aaatatactt ataaatatgc ttacaaaatt
      181 agctaatatt tccgttgaca aagctttgtt actattgtta aactaaattg actagcaaaa
      241 gtgaaacata aatttattaa ataaatgtaa aaattaggag aaatcaatgt cttatagagt
      301 acaattaata atttcagaag atgtagaaag tcagcaattt ggcactaatg ttattaataa
      361 agtaattaat ccagctcttt ctattaatgc gcctttaatt cctactgctt tatcattttc
      421 tgtaacagct gtggtgtctg agatagagga tacagaaaag ataaaaatag tagaaattga
      481 agttttaaac aagaatgaaa aacaaatttt ttctacgggt gaagtatcgg taaacttgcc
      541 tcctcaagtt aacgatataa actttaatat aaacgccaga aatgtcttgg ttgaagaagc
      601 aggagagcat tatgctgttg ttaaatttaa tggaactgag attggaaggc aaatctttga
      661 tatcaaggtc aacaaaccag tggaaaaaaa ttaaggagac gatacggatg cttgatatag
      721 taccatcaaa tacacatcgg agtggtagct taataaattt tgctgcgatt tttgcttgcg
      781 catcggtaat tctagcatct ccagttgttg tggatgcaag ttctactccg aaaactaaaa
      841 atgataatca acttgtaatt actacaaaaa atgagattaa cacaatttca agtaatgatg
      901 gcaaaatttt tgatgtgata aatacggttt taaaaagaaa agctgaaatc aatataaaat
      961 atgatgaaga tttaaacttg tatttctttg ttatcaaaac aacatctgaa ttgttttcct
     1021 cagattacga tgttttggat actctcgata atgtattatc tgactataaa tatatgggga
     1081 aatctgtagt agcaactttg ggggaataaa atgtttgact ttaatgaata tttatctatt
     1141 tccaaacaaa ttgaatcaga tgataaatat aattctaaag aatcttatag aagaactgct
     1201 gtttcaagag catactacag tgcttttaaa aaatctgatg agtatttgaa agaaaattat
     1261 gatattattt ataatggaag tagcggaaaa ggaagccacc aaaccgtttg gaatttgttt
     1321 tcaacagtta aggaattgaa taccttagga atacataata gtgggtatag aatgttagag
     1381 aaaagaaaat gtgctgacta tattcctaat gaaaccatta cgaaaacaga tatggcgctg
     1441 atgaatcgag aagcagaaaa aataataaac aaactcacct aatgaatttc aagcgagttt
     1501 gtttgttatt ttttt
//
LOCUS       DACGLW010000164         1467 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.175, whole genome shotgun sequence.
ACCESSION   DACGLW010000164 DACGLW010000000
VERSION     DACGLW010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1467
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.175"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            82..492
                     /locus_tag="IXT53_002899"
     CDS             82..492
                     /locus_tag="IXT53_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HAQ4906656.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            506..862
                     /locus_tag="IXT53_002900"
     CDS             506..862
                     /locus_tag="IXT53_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HAQ4906657.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          540 a          211 c          271 g          445 t
ORIGIN      
        1 ttttatattt tattagctaa taattggtat tttaaaagaa attactgtag aattggatta
       61 taattaaaaa ggagtgagaa aatgataaaa gtatatactt cgcctagctg tacttcctgt
      121 agaaaagcaa gagcatggtt gcaagcgaat caacttgaat ttgaagagaa gaatattttt
      181 gccgaaccgt taacagaaag tgagatcaaa aagatcttgg cagcgactga aggaggagtt
      241 caagatatta tttccactcg gtcgaaaatt tacgaaaagc tgggcataga ttttaacgaa
      301 ttaactttaa agcaaatgat cgtcttattc aaagaatatc cttcattatt gagacgacct
      361 attttgattg atgaaaaaag aatactaatc ggatttaatg aagatgagat ccggtcattt
      421 gtccctcgtg aaattcgatg tgttgccaga aatctgatct atttacaaaa caataacatc
      481 ttaaattcat agggagggag ccttcgtgga agtgaaagtc tatggatcag cgcactgtgt
      541 aacaagtctg aaggcgaaac aatggttaga acaccatcaa ttgccttatc aatttgttga
      601 cttagaagaa cgcgatctaa gtacagaaga agcagaagaa ttatgcagtt ttgaagaagt
      661 gcaagcagaa cagctatttg cgacttggtc tgaggctttt cacaacattg cgattgacct
      721 ttcctgtgta ggaaaaaaac aactgatttt tttatgcaca caacatacca atctcctcag
      781 acgtcctttg attattatcg atgaagttct atttattgga tacaatgagc agttgatgga
      841 acagcttatc ttaaataaat agactttaac tgttttaatc aagtcactga gcatttcata
      901 cagttgtaac agtcgaacac ttagcatcaa gcgtgttatg tgttcttttt ttgactttat
      961 ttttctacta tagagacatg ctcaatttat gtggtcaaaa ttcaaatcta ggtggggata
     1021 tagagttaaa aatatattga catgtgtaaa tttacatgat atattcattt ctgttgtttt
     1081 aagtagcaaa gcaataaaat agtattttaa aaaacatata aaatagttgt tgacatgtta
     1141 gaaatgacat gataagataa ctgagttgtt aaaaaagaac cacttgtgaa gcttgattct
     1201 atcgaaaaaa acttcaaaaa agttcttgac aaataacaaa tgataagtta tgataaatga
     1261 gttgctgtaa gatttcgaaa aacgaaatca aaaaaacttt taaaaaagag ttgacaatta
     1321 cttgtctctc tgatatgata taaaagttgc tacggcagcc aaaaacgaat cggggaacac
     1381 tcctgatgaa gtagaccttt gaaaactgaa caaagtaaga caaaccaaat gtgtagggcg
     1441 tcttgattca attcaagaca acaaaca
//
LOCUS       DACGLW010000165         1458 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.176, whole genome shotgun sequence.
ACCESSION   DACGLW010000165 DACGLW010000000
VERSION     DACGLW010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1458
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.176"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            <1..354
                     /locus_tag="IXT53_002901"
     CDS             <1..354
                     /locus_tag="IXT53_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HAQ4906658.1"
                     /translation="TQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVD
                     QEMYKQAQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISI
                     LKELEEHENWYAEVE"
     gene            355..903
                     /locus_tag="IXT53_002902"
     CDS             355..903
                     /locus_tag="IXT53_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906659.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            1163..1458
                     /locus_tag="IXT53_002903"
                     /pseudo
     CDS             1163..1458
                     /locus_tag="IXT53_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          575 a          182 c          311 g          390 t
ORIGIN      
        1 acccaagtaa aatcgatgag aaatgcgatg caatatcttg agtatatgga cttttttgtg
       61 aaagaatttg atacaaattt atatgttgct ggtgttattc ctttcatgtt ggaaagcggt
      121 gacgcagtag atcaggaaat gtataaacaa gcccaagaga tttatgggga acatttgatt
      181 aaaaatgttg tactcaaaaa tgcgcgctta aaacgatacg acggatcagg tattacgatg
      241 gagaagacaa agaaaggtct cttaaaacaa tgggatcgca aaagccacga attatttatc
      301 agtattttga aagaattgga agaacacgag aattggtatg cggaggtgga gtaattggct
      361 gacatcaaag gacttgatat ttttgggaac aacaaaggac tcgccaaaat aaaaaaagaa
      421 gcagaagcaa agaaaaagca gcaagcaatt acaccatctg aaaataagca attagaagtg
      481 caccaagttc aagaaataaa aacttcctcc aatgttgaaa gacagagtag aggaagaaca
      541 aaaaaagttg gcgcaccaat cagaaagttt gataaggttt attccaccaa gcatccttta
      601 aaactgtcga ctttgttaaa tgcgacttcc agagttttag tagaaaagta tgagactaat
      661 atgacaagag atgaattgct tagaaaagct ttagatgagt atattaaaca gaatcttacg
      721 caagaagata agcaagattt gtttaatgac gtggtgaaag aactagactt gttcagagaa
      781 aaatatccaa ttgttccaga attagatgat cgtggagaaa taataagaac taccagtcag
      841 ataaaacaag ataccgaaga ctcactgcgc aaagggtggg gaatgaataa taaagttaga
      901 taataatcaa agaagaacta agtaactacc tttaaacggt agttacttag ttcttctttg
      961 atttgcatga aatagagggg atttattttt ctggttacgt gattaaaaaa tccaaaaaat
     1021 gagcatagga acggttatga ttttagggtg tgttttaatg agatattgtg atttcattaa
     1081 caagtgtatg ataatctgtt gttgagttag taaataagta atagagctaa ctctaaaata
     1141 aaactattag gaggagatgt aggtgaaaaa gtttaaaaag aaaatatatt taggattggt
     1201 tgttggggtg agtatgtttg ggttcatggc tcatactaaa caagtaactg cagatactaa
     1261 ccaaaaagaa aggattatga gccaattaga tgactctcaa ataagtatgt acccaatgag
     1321 ctaattgtca gtttaaaggc tacacaaaga atttcaacag agaaaaataa accgcaaaat
     1381 ttattgggtg taaaagttat ggtggaaaaa gatttgactc ttaggacaaa tgaaagcgat
     1441 tgtaataagc gagatcaa
//
LOCUS       DACGLW010000166         1457 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.177, whole genome shotgun sequence.
ACCESSION   DACGLW010000166 DACGLW010000000
VERSION     DACGLW010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1457
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.177"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            109..1041
                     /locus_tag="IXT53_002904"
     CDS             109..1041
                     /locus_tag="IXT53_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HAQ4906660.1"
                     /translation="MLKLKEKEYIIDDAVSVPLILNKREKNNLKKKMLIPFDSEVKGL
                     LNKLTISNIDSTREKIIKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITE
                     EKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKEL
                     YANKIQELEEKLKELEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEP
                     MDRIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLD
                     EIEKVVLENYNGTVTFTKLAKAEQYRRSLELSKD"
     gene            1113..>1457
                     /locus_tag="IXT53_002905"
     CDS             1113..>1457
                     /locus_tag="IXT53_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906661.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQD"
BASE COUNT          640 a          169 c          228 g          420 t
ORIGIN      
        1 gatgaaataa atcaaaagta tgatgaaaca ttaaaaaatg tctattcttc acttatcgtt
       61 tcaaatatag aagaaacttc atcttctgaa ataaaaaata agctttcttt gttaaaatta
      121 aaagaaaagg aatatattat tgacgatgct gtttctgtcc ctcttatttt aaataaacga
      181 gagaaaaata atttaaagaa aaaaatgctc attccatttg attcagaagt gaaaggatta
      241 ctcaataaac taactatttc taatatagac tcgactaggg aaaaaatcat aaaaatattt
      301 gataaaatta ataatttatt caagaatgac aatgctgaat taaggaaggg tcttttagag
      361 tttaaacttc aagaactaga acttcattac tcttatttag tgaaaattac cgaagaaaaa
      421 gaacaacaaa aagcaataaa agaacagatg atagaagagg aaaaagttcg tagagaaatt
      481 gatcgtgaaa agaaacgtat tgataaagaa caacgtcaat tcaattctga aatttccaaa
      541 ttaatcgcat atatgcaaaa agctaatgca gatgtggaaa aagaattata tgcaaataaa
      601 attcaagaac tggaagaaaa attaaaagaa ttagaagtta tacgtgaaaa tgtattacaa
      661 agagaattaa atactagagc cggttatgtg tatgtaatta gtaatattgg gtcttttggt
      721 gaagacatat ataaaattgg tatgactaga agattagaac ctatggatag aataaaagaa
      781 ttgagtagtg catcggttcc atttgaattt gacgttcatg cgatgatttt ttcagaggac
      841 gctccgtctt tggaaactaa attgcataat cattttagaa aacaagaagt taataaaatt
      901 aatcaaagaa aagaattttt caaagtttca ctagacgaaa tagagaaagt tgttttagaa
      961 aattataatg gaacagtaac atttacaaaa ttagctaaag ctgaacaata tagacgatct
     1021 ttagaattgt caaaagatta aatatataaa aagccttcgg gctttttctt ttaacaatta
     1081 ataggaacgt atattcgaaa ggaatagaaa taatgtggtt agaaaaaata aatgacaaaa
     1141 aatttaagta caccgaacgt tatacggatc ccttaactga aaaaaagaga aaagtgtccg
     1201 ttactttatc tagcaattca cgccaagctt ggaatcaagc taacttactt ttgaatgaaa
     1261 aaatcgcaga aaaaataaaa cgaaatgaag aattaccact aacttttgga gaattaaaaa
     1321 ctaaatggga tgaaaaatac aagccaactg taaaagaaag ttcctatcga acaactcagg
     1381 tttatctatc attaatttca aaatatataa aagatgatgt acttgtaaaa aacgttaata
     1441 gtaatcttat acaagat
//
LOCUS       DACGLW010000167         1455 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.178, whole genome shotgun sequence.
ACCESSION   DACGLW010000167 DACGLW010000000
VERSION     DACGLW010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.178"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            75..1415
                     /locus_tag="IXT53_002906"
     CDS             75..1415
                     /locus_tag="IXT53_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ4906662.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
BASE COUNT          462 a          321 c          284 g          388 t
ORIGIN      
        1 taataaacgc aaacagacac cttgaaatcc tttagaataa agtcgacgaa aaccattcaa
       61 aggagtgttt caagatgtcc tattcagagt ttaccaaaga gttactagat attctagacc
      121 taaatcttac ctttcatgaa gatgcctttc gcaaagagcg cattaatgac gaaacatgtt
      181 ttgtttttga tggcacattg acctatcaac cagaggaatg cttccattgc cactatcaaa
      241 ataagcaaac gatcattaaa tggggctgga aaaaagtatc catcttattg aatgatgtca
      301 gtaactacaa gaccattgtg cggattaaca aacagcgctt caaatgtaaa cactgtggaa
      361 agactttttt agcagaagat tcagttagcg atcgtcgctg ctccattgcc cgcagagtca
      421 aacaagccat tcttgagcta ttaagtgagc ctatttctat gtctttgatt gcacgtatga
      481 agcacatttc tccgaccact gtcattcgaa tacttcgcag ccttcggcct aaaaccgttt
      541 ctttgaacca gccgttacca gaagtggtgt gtttcgatga attcaaatcg gtgaaaaacg
      601 tctcgggagc catgagcttt gtcatgatgg acggcaagac ccatcaactg attgatattg
      661 tggaaaatcg gcaactcaat cctttacgcg actatttctt gcgctaccct agaaaagtcc
      721 gtgaacaagt acgtctagtg gtttcagatt tctatacacc ctatcgcaca ttggtgaaag
      781 agtgttttcc gaatgcccga atcgtcgccg atcgctttca tattagccaa cacatcggtc
      841 gggcctttac caaccaccga attcaagtaa tgaagacctt taaaaaaggg gatcgtcggt
      901 cgaagcactt aaagaaatac tggagactac ttcaaaagaa tgcgtgggaa ttgaatggtc
      961 agcatcgcta ctggcgtccg tcatttcgag atcatctgac agaagcggag atagtcgatc
     1021 ggctattata ttatgatgac tccttaaagc gaggctacga agtctaccag gtttttcttt
     1081 ccgctatcag aagacaagat gttcctgaat tcgtcgcgct cctaaaagaa gactacaagg
     1141 agttgccaga gcactatcaa ccagtgttca cgaccttcaa gaaatatcga acagaaatca
     1201 aacgagcgtt gcgagttccc tattccaacg gccctataga atgtttgaac aaccatatca
     1261 aagtactaaa acgaattgcc tatggcttta gaaactttca aaactaccga gaacgaatct
     1321 ttttatatcg aggaaaatat ttcaagaaaa ctaagaacac tacccaactg actaaagcca
     1381 gaacaacaac acggctagac aagatagctg tctagccgtg tgagtatctc gattattcta
     1441 ttgaatttct tcacc
//
LOCUS       DACGLW010000168         1445 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.179, whole genome shotgun sequence.
ACCESSION   DACGLW010000168 DACGLW010000000
VERSION     DACGLW010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1445
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.179"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            <1..226
                     /locus_tag="IXT53_002907"
     CDS             <1..226
                     /locus_tag="IXT53_002907"
                     /inference="COORDINATES: protein motif:HMM:NF018513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HAQ4906663.1"
                     /translation="LWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKIIKEAIPSLLLI
                     VIIIGGILTGIFTAVEASAIAVIYKWVIRF"
     gene            208..513
                     /gene="kduI"
                     /locus_tag="IXT53_002908"
                     /pseudo
     CDS             208..513
                     /gene="kduI"
                     /locus_tag="IXT53_002908"
                     /inference="COORDINATES: protein motif:HMM:NF002091.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
     gene            533..1336
                     /locus_tag="IXT53_002909"
     CDS             533..1336
                     /locus_tag="IXT53_002909"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HAQ4906664.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIRDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
BASE COUNT          462 a          259 c          344 g          380 t
ORIGIN      
        1 tttgtgggcg ctaggaatca tgctagtagc attcatcgtt gctaaaaaga ataaataccc
       61 tgttgtcgaa aaaggacaga tggctcatgc tggaaaaata ataaaagagg cgattcctag
      121 ccttctattg attgtcatca ttatcggtgg tatcttgacg gggatattta cagcagtaga
      181 agcttcagcg atagctgtca tttataaatg ggttatacga ttttagaacc aggaagtgct
      241 tggaatacga tgccttgtca tacacatgaa cgtcgtatgg aagcttatgt ctattttgat
      301 tatgccaatg aagatacacg tgtgttccat atgatgggca aaccagatga aacaaaacat
      361 ttagtcgtag acaatgaaca agccattatt tcgccaagct ggtcgatcca ttcaggtgta
      421 ggtacaagca attactcatt catctgggca atgtgcggag aaaatatcac ctatacagac
      481 atggatatgg tgcaaatgga tcaattaaaa taagcataag ggagagaaaa tgatggaatt
      541 tagtatggat atgtttcgtt tagatggaaa agtagcatta gtcaccggtg ctgtctatgg
      601 tattggattt gagattgcac gctctttagc tacagcagga gcaaaaatcg tatttaataa
      661 cttgaatcaa gaatcagttg atgaaggtat agcgcattat aaagaagcag gcatcgatgc
      721 aaaaggttat gtctgcgatg tgaccgatga agaagcggtt caagcgatgg ttcaacagat
      781 caaagaagat gtaggttcag ttgatattct agtcaacaat gcagggatca tcaaacggac
      841 accgatgatc gaaatggatg cggcagactt ccgccaagta atcgacgtgg atttaaatgc
      901 cccattcatc gtttcaaaag cagttattcg ggatatgatc gaaaaaggcg gcggtaaaat
      961 catcaatatc tgctccatga tgagtgaatt aggacgcgaa acggtcagtg cttatgctgc
     1021 ggcaaaaggc ggattaaaaa tgctgacgaa aaacatcgct tctgaatacg gtcaatacaa
     1081 tatccaatgt aatggaattg gaccaggtta tattgctaca ccgcaaactg ctccattacg
     1141 cgaaacacaa gaaaacggtg aacgtcatcc attcgatcaa tttatcgttg gacgtacgcc
     1201 agcagcacgc tggggcgagc caatcgattt ggctggacca tctgtcttct tggcttctag
     1261 cgcatctgat ttcgttaacg gccatgtctt gtatgtggac ggaggcatac ttgcctatat
     1321 cggaaaacaa ccttaaaaga gctttagtac accgtttagt ggagaggttg tgaaatcagc
     1381 ggcttgaacg aataccgaag gacagctgat taaacatcat ttattcgaaa aaagaggctg
     1441 ggaca
//
LOCUS       DACGLW010000169         1424 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.180, whole genome shotgun sequence.
ACCESSION   DACGLW010000169 DACGLW010000000
VERSION     DACGLW010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.180"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            complement(150..1337)
                     /locus_tag="IXT53_002910"
     CDS             complement(150..1337)
                     /locus_tag="IXT53_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HAQ4906665.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          414 a          309 c          249 g          452 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttcact ttta
//
LOCUS       DACGLW010000170         1420 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.181, whole genome shotgun sequence.
ACCESSION   DACGLW010000170 DACGLW010000000
VERSION     DACGLW010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1420
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.181"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            4..978
                     /locus_tag="IXT53_002911"
     CDS             4..978
                     /locus_tag="IXT53_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase family protein"
                     /protein_id="HAQ4906666.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTITPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          495 a          184 c          236 g          505 t
ORIGIN      
        1 actatgtgta cgtctattac ttatgtaaca agtgatcatt attttggaag gaattttgat
       61 tatgaaatat cttacaatga agtagtcact attactccaa gaaattataa gttgaatttt
      121 cgaaaggtaa atgatttgga tactcattat gcaatgattg gtattgccgc tggtatagct
      181 gactaccctc tttattacga tgcgacaaat gaaaaaggat tgagtatggc tggactaaat
      241 ttttctgggt atgctgatta taaagaaata caagaaggga aagacaatgt atctcctttt
      301 gaatttattc cttggatttt aggacaatgc tcaacagtag gagaagctaa aaaattgtta
      361 aaaaatatca atttagcaaa tataaattat agtgacgaac ttcctttatc ccctttacat
      421 tggctattag ctgataaaga aaaatcaatt gtcattgaaa gtatgaaaga tggacttcat
      481 atatatgata accctgtggg cgttcttacc aataatcctt catttgacta tcaattattt
      541 aatttaaaca attatcgtgt cttatcgagt gaaactccta aaaataattt ttcaaatcaa
      601 ataagtttga atgcctatag ccgcggtatg ggagggatag gcttgcctgg agatttatcc
      661 tcagtatctc gttttgttaa agcgactttt acgaagctga attctgtatc tggagattca
      721 gagtcagaaa gtattagtca atttttccat atcttaggtt cagtagaaca acaaaaaggt
      781 ttgtgtgatg ttggtgatgg aaaatatgaa tatacaattt attcttcttg ttgcaatgtt
      841 gacaaaggaa tctattatta tcgaacatat gaagacagtc aaattactgc aattgatatg
      901 aataaagaag acttagatag tcataagtta attagttatc caattataga aaaacaacaa
      961 attaaatata taaattagtt aatgtgttgt gattgattat ttaatataga tataatacaa
     1021 aaaggcaaag atattttcaa aacatttttt cttgatgtga tatccttgtc ttttttgtaa
     1081 tgtttttaac ttattgcttt tgcaaaattt atttacctgt tttctggatc aacgatagtg
     1141 tatattatgc tatccttgtt tggattttag gacgtttaaa gaggcacttt tttacataac
     1201 ttaatccata gaccttttaa gcatgctgtg agataataat aaagaagagt ttaaagcgca
     1261 ccccaaacca cttccccata agtgtattac gctttaaact cttttatatt tgaattcact
     1321 aagaagcata ccatattttt gaaaaaaagt gagaaaaaag gcttataatt aggatttaga
     1381 gtaattaatt agatgtttat tttataggac tgatactata
//
LOCUS       DACGLW010000171         1409 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.182, whole genome shotgun sequence.
ACCESSION   DACGLW010000171 DACGLW010000000
VERSION     DACGLW010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1409
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.182"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            <1..1359
                     /locus_tag="IXT53_002912"
     CDS             <1..1359
                     /locus_tag="IXT53_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HAQ4906667.1"
                     /translation="LFSDEEISVSVPEAAATIIPVKGHQRVVGTKADKIKHLPITEKE
                     HLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCLTHYQHTYCCKSCEKEGLSS
                     FKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQENYWKQTHGLDISRDNITNW
                     HIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESAKTDTYYWQFCTGKDSQHPI
                     VYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESVRLVYCFAHVRRKFFEAIPK
                     GKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQQELRPLFIAFYEWMATIDP
                     VAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERNIKELVIGRKNWLHSTSLEG
                     ARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLSAETLDQLLPWNKDVQQHFS
                     RN"
BASE COUNT          466 a          255 c          299 g          389 t
ORIGIN      
        1 ctttttagtg atgaagaaat ttctgtaagt gttccagaag cagctgccac cattattcca
       61 gtaaaaggac atcaacgagt tgtaggaacg aaagcagaca aaatcaaaca cttacctata
      121 acagagaaag aacatctcct tcctttggaa gaacaatttt gtgaacattg cggttcacag
      181 atgaaagata tcggacggac aaaagtacga gaagaaattc gtttccatca ggctatgttg
      241 gattgtctca cccattatca acatacctat tgttgtaaaa gctgcgaaaa agaaggactt
      301 tcttctttta aaaaagcgat cgttcctaaa cctttgattt ctaatagctt aggttccaat
      361 agtttagtgg cggaaacgat ccggatgaaa tttggtcaga aagttcctgc ctatcggcaa
      421 gagaactatt ggaaacaaac acatgggttg gatatttcga gagataatat caccaattgg
      481 catattaaag cagtgcaaaa cgcgttagac cctttaggag aacgattaag agtctatctt
      541 aatcaagaag agattctaca cggggatgaa acaagttatc gtgtgattga aagtgccaaa
      601 acagatacct attactggca gttttgtaca ggaaaagaca gtcagcatcc catcgtttat
      661 tatcaccacg acgaaagtcg tgctggagat gttccgaaaa cttttctcaa ggagtttaca
      721 gggtatttac actgtgacgg ctatagcggg tataatgccg tggaaagtgt acgacttgtc
      781 tattgtttcg cgcatgttcg gcggaaattt ttcgaagcaa ttccaaaagg aaagaaaaac
      841 acggatattc ccgcagctca agcagtcaaa caattggata aatggtttgt cctagaaaag
      901 aaatggaaag acttttctcc tgagaagcga ttaagctgtc ggcaacaaga actgcgtcca
      961 ttatttattg ctttttatga gtggatggca acgatagatc ctgttgcaaa atccaagttg
     1021 gatgcagccg ttcaatatgc gtgtaaacta cgaagtggtt ttgaacctat ttttgaagat
     1081 ggtcgtttag aactcacaaa taatcgtgcg gagcgaaata tcaaagagct ggtgatagga
     1141 cggaaaaatt ggttgcattc cacaagtctt gaaggagcac gaacttcagg aatcattctg
     1201 agtgtatata aaacagcaga acttaacgga ttgaatcctg taaagtatct agagtttcta
     1261 tttgataaga taccaaatct tcccgtactt tcagcagaaa ctttagatca actgttgcct
     1321 tggaacaagg atgtacaaca acatttttct cgtaactaaa aacgccaaac attcccgatt
     1381 ctattctata ggtcgggtta tttgacgta
//
LOCUS       DACGLW010000172         1359 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.183, whole genome shotgun sequence.
ACCESSION   DACGLW010000172 DACGLW010000000
VERSION     DACGLW010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1359)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1359)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1359
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.183"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            <1..484
                     /locus_tag="IXT53_002913"
     CDS             <1..484
                     /locus_tag="IXT53_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ4906668.1"
                     /translation="KEAIDQIKTAVLYPHNGLPILLMGVSGSGKTFLAEKIYEYAKEQ
                     QVIESNAPFIAYNCAQYFNFSINKIVENLTILDAMKVLRLVQKAVKELEKLMGIEFSN
                     NQLFLLYMHCCSMIERILRKESVDEQADIKEYIQKEGHNYEISKYPAYKTKFESTVIK
                     "
     gene            complement(691..1092)
                     /locus_tag="IXT53_002914"
     CDS             complement(691..1092)
                     /locus_tag="IXT53_002914"
                     /inference="COORDINATES: protein motif:HMM:NF013068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906669.1"
                     /translation="MSDKFSKKISSDIKEDFKAIFKTASIELAGERKQLIMDKYGEDK
                     KVATACKILDEGFEDAMQVMVLPENIRQRLRTTNVLERLNEEVRRRERVVRIFPNCDS
                     VIRIIGALLMEKDDEWTSAPRKYLEFDRNEI"
     gene            complement(1213..1296)
                     /locus_tag="IXT53_002915"
                     /pseudo
     CDS             complement(1213..1296)
                     /locus_tag="IXT53_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
BASE COUNT          445 a          254 c          260 g          400 t
ORIGIN      
        1 aaaggaagcg atcgatcaaa tcaagacggc tgtcttgtat cctcataacg gattaccgat
       61 tttattgatg ggtgtaagcg gttcaggaaa aacctttttg gcagaaaaaa tttatgaata
      121 cgccaaggaa caacaggtga tagaatcaaa tgctccattt atcgcctata attgtgctca
      181 atactttaat ttctcgatta acaagatcgt agaaaattta acgatccttg atgcaatgaa
      241 ggttttgaga ttggtacaaa aggccgtcaa agaactcgaa aaactaatgg ggatcgaatt
      301 ttcaaacaat caattgttct tgctttacat gcactgctgc tcaatgatcg aacgtatttt
      361 acgcaaagaa agtgtagatg aacaagcaga tatcaaagaa tatattcaaa aagagggaca
      421 taattatgaa atatcaaaat acccggctta caaaacgaag tttgaaagta ctgtaataaa
      481 gtaagattta aataagcata aacaaggtaa gaaccatagg ggctgtgtca agtccaaaat
      541 cttgtgtaaa aaggaaatct caaagtacga tgttttgcct tgttgagcct agctacggtc
      601 caatatgctg gtaagaagtg tcaagagcaa aaagcgaagc gtctcttgat acttctcacc
      661 agtatattat actattggcg gcttaatgtg ctaaatctcg ttacgatcaa attcaagata
      721 ttttctaggt gcagaagtcc attcgtcatc cttttccatc aataaagcac cgatgatacg
      781 aataacagaa tcacaatttg ggaaaattcg gacaacacgt tcacgacggc gtacttcttc
      841 atttagtctt tcaagaacat tcgttgttct caatcgttga cggatatttt ctggtaaaac
      901 cattacttgc atggcatctt cgaagccctc atccaatatt ttacaggcag ttgcgacttt
      961 cttgtcttcc ccatatttat ccataataag ttgtttacgt tctccagcca gctcgatgct
     1021 tgccgttttg aagatggctt taaagtcttc tttgatatcg cttgagattt tcttggagaa
     1081 tttgtcggaa atgttgcgaa ggagatgtgc ttggcatctc tgctagctcg cacaaagaag
     1141 gtattctttc acagcttttg tgagccctat atgcgcatcc gagatgacca tctttactcc
     1201 tgaaagaccg cgttctttca atccgagagt gtaaaatatt ttgtgtaaat agaaaaaagg
     1261 aagtcccttc agtagaatag agttaccaca acacattcac agaaaagagg acttccatat
     1321 gaacgatttt actacagaaa ttctaaagac tctagcgaa
//
LOCUS       DACGLW010000173         1356 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.184, whole genome shotgun sequence.
ACCESSION   DACGLW010000173 DACGLW010000000
VERSION     DACGLW010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1356
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.184"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            21..1322
                     /locus_tag="IXT53_002916"
     CDS             21..1322
                     /locus_tag="IXT53_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ4906670.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          439 a          270 c          252 g          395 t
ORIGIN      
        1 caatcaaagg agcgttctgt atgcctgatc ttcatcatat caaactacta cttggaatca
       61 aagataaaaa cattttcatc acaaatgtgg aatcgaagag cattaagaat atcaagagtc
      121 ttgttgtttc tgcgacgtta agtaaagaaa ttcgtcgctg tcctttgtgt aaacagatga
      181 atcatgaagg catgatcgtt aagaacggaa agaaaaaatc gctcattcaa cttaataaat
      241 gtgcgaacca actgacttat cttgctttag cgaaacaacg ctaccactgt cgtggctgcc
      301 acacctattt tacggcgaat acctacattg tcgatagaaa ctgctttatc gctaaacaag
      361 tgcgctataa aattttagaa gagctaactg aaaaacaagc aatgacgaca attgctaagc
      421 attgcggcgt ttcttggtcg accgtttctc ggacactcgc ttctctgatc cccatgacaa
      481 aagtaaaaag aaattggtta ccgcgttgtt tattaatgga tgaatttcgt tcactgaaaa
      541 atcaagtcgg tccctactcc ttttcttgta tggatggcga tactggaaaa ttactggata
      601 ttcttccttc acggaagaaa aaggctctcg tttcttattt tatgcaattt gaacgacgcg
      661 cacgattgaa ggtaaaggtc atcgtgacgg acatgaatgt ttcttatgcc tctctgataa
      721 aggaatgttt tccaaaggca aaactcgttg tggatcgttt tcacattgtc aaacatctca
      781 ttcgtaaatt tgaggatatt cgcgtacgaa tcatgaaaag ctttgatcga aacgatccaa
      841 cacaagccaa atattatcga caattaaagg cgttgtctcg gcttctgatc aaacgacagg
      901 acaagcttgt ttatgacaaa tggaccaaat ggcgcaattt tggttgggcg tatctcaccg
      961 aaagtgaagt ggtggatcgt ctgctttcga tctctgacga attacgaatc gcctattttt
     1021 actatcaaga aattcttcaa gcgtttcacg acaaagaggc ggacactttc tttaaacttg
     1081 taagaacgat gcccaattcc gttccaaaag aactgcattc tataaaaaaa gcttttataa
     1141 actatgaatc cggcattcgt ttagccctcg aattgcctta ttcaaatgcc aaaatagaaa
     1201 atctgcatac ccacatcaaa gcattgaaac gagtcgctta tggctttcga agctttcgga
     1261 aaatgaagac cagaattttc ttactaaata atctgattac ctatgaatca aaaaacatct
     1321 aaagtagaaa aggaattcta ccttagatgt tcttat
//
LOCUS       DACGLW010000174         1356 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.185, whole genome shotgun sequence.
ACCESSION   DACGLW010000174 DACGLW010000000
VERSION     DACGLW010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1356
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.185"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            <1..1343
                     /locus_tag="IXT53_002917"
     rRNA            <1..1343
                     /locus_tag="IXT53_002917"
                     /product="23S ribosomal RNA"
BASE COUNT          369 a          295 c          409 g          283 t
ORIGIN      
        1 gaaataatag taccgaagtt cctgatgtca cactgccgag aaaagcttct agtgagaaaa
       61 cagcggcccg taccgcaaac cgacacaggt agtcgaggag agaatcctaa ggtgagcgag
      121 agaactctcg ttaaggaact cggcaaaatg accccgtaac ttcgggagaa ggggtgctga
      181 tcatacgatc agccgcagtg aataggccca agcgactgtt tatcaaaaac acaggtctct
      241 gcaaaatcgt aagatgaagt ataggggctg acgcctgccc ggtgctggaa ggttaagagg
      301 agtgcttagc gcaagcgaag gtacgaattg aagccccagt aaacggcggc cgtaactata
      361 acggtcctaa ggtagcgaaa ttccttgtcg ggtaagttcc gacccgcacg aaaggcgtaa
      421 cgatttgggc actgtctcaa cgagagactc ggtgaaattt tagtacctgt gaagatgcag
      481 gttacccgcg acaggacgga aagaccccat ggagctttac tgtagtttga tattgagtgt
      541 ctgtaccgca tgtacaggat aggtaggagc cgtagaaatc ggaacgctag tttcgatgga
      601 ggcgctggtg ggatactacc cctgcgttat ggccactcta acccgcacca ctaatcgtgg
      661 tgggagacag tgtcagatgg gcagtttgac tggggcggtc gcctcctaaa aggtaacgga
      721 ggcgcccaaa ggttccctca gaatggttgg aaatcattcg aagagtgtaa aggcagaagg
      781 gagcttgact gcgagaccaa caagtcgagc agggacgaaa gtcgggctta gtgatccggt
      841 ggttccgcat ggaagggcca tcgctcaacg gataaaagct accctgggga taacaggctt
      901 atctccccca agagtccaca tcgacgggga ggtttggcac ctcgatgtcg gctcgtcgca
      961 tcctggggct gtagtcggtc ccaagggttg ggctgttcgc ccattaaagc ggcacgcgag
     1021 ctgggttcag aacgtcgtga gacagttcgg tccctatccg tcgcgggcgt tggaaatttg
     1081 agaggagctg tccttagtac gagaggaccg ggatggactt accgctggtg taccagttgt
     1141 tctgccaagg gcattgctgg gtagctatgt agggaaggga taaacgctga aagcatctaa
     1201 gtgtgaagcc cacctcaaga tgagatttcc catttcttta agaaagtaag atccctgaga
     1261 gatgatcagg tagataggtc aggagtggaa gtacagtgat gtatggagcg gactgatact
     1321 aatcgatcga ggacttaacc aataggttaa aagaaa
//
LOCUS       DACGLW010000175         1354 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.186, whole genome shotgun sequence.
ACCESSION   DACGLW010000175 DACGLW010000000
VERSION     DACGLW010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1354
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.186"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            82..>1354
                     /locus_tag="IXT53_002918"
     CDS             82..>1354
                     /locus_tag="IXT53_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HAQ4906671.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLI"
BASE COUNT          460 a          261 c          257 g          376 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atca
//
LOCUS       DACGLW010000176         1334 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.187, whole genome shotgun sequence.
ACCESSION   DACGLW010000176 DACGLW010000000
VERSION     DACGLW010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.187"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            86..1165
                     /locus_tag="IXT53_002919"
     CDS             86..1165
                     /locus_tag="IXT53_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ4906672.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
BASE COUNT          359 a          357 c          369 g          249 t
ORIGIN      
        1 agccatttgg aaataaaaaa acgccacgtg aattccatgt tatattgaag ttaccacaac
       61 tctcaataga caaggatgaa ttcacatgac gcaaaaccat gataacacag tttcacgcaa
      121 gggaaaacac ctaacctact cagaacgttg ccaaatcgcc atcctgaaaa aggaaaacta
      181 ttccaaccgt caggttgcgg atgtcctggg ctgcgtgccg cagaccataa acaacgaaat
      241 caaacgcggg accgttactc agcttaaccg ccagaatcag aacgggaagg tctacgacta
      301 ttatcatgac gtctacgacg ccgatgccgg gcaggcggcc tacgagaaga accgcttgaa
      361 ctgcggcaga cggcccttat gggccgacac ggacgccttc attgaatggg cggacgacat
      421 gatgctgttg gaaaaatggt ccccggacgt ggtggtgggc tttgccagaa aacacgaatt
      481 gttcgacccg gccattatcc ctgggaccac caccctctat cagtggatcg accgtggcat
      541 catgaaaacg aagaatatgg acctgctaga aaaactgtcc cgcaaaccga aatcgtcccg
      601 tcatggcaac cgcccgaata aacgggtgct agggaaatcc attgaagagc gcccggaggc
      661 ggttggtacc cggaaaacct tcggccattg ggaagtcgac acggtcgtag gaaacaagac
      721 caagtcggat gccgtgctgc tgacgttggt ggaacggcag acacgcttcg aggtcattct
      781 gaagctggcc gggaaggatg ccgaatcggt caaccaagcg gtccgacagc tgagagaacg
      841 ggctggtgcg tacttcggcc agctgttcaa gacggttacc tcggacaacg gctcggagtt
      901 cgcgggactg cacgatgcct tgcaggatgt ggcaaacatc tacttcagcc acccctatgc
      961 ttcctgggag cgcgggacga gcgaaaacca acacaagctg attcgccgtt tcatccccaa
     1021 gggcagccca atcggcaacg tgagcgaaaa ccaaatcttg cggattcagc gttggatgaa
     1081 cgattacccg cgaaagattt tgggctatgc aacgccgcat gacacgttcg tccaggcatt
     1141 taggcaggaa cggttgacgg cttgacacgt gggcccttga caacgagcct ccctcggcat
     1201 ggcttgtgtc tggaaagctg gccggggcca gccagtcggg agactaccat gcctcagctc
     1261 attgtcaagg gcccactcga ttagaaaaat tagacggttg gtggtaacga gcaaccgggt
     1321 caccatgggt ggct
//
LOCUS       DACGLW010000177         1334 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.188, whole genome shotgun sequence.
ACCESSION   DACGLW010000177 DACGLW010000000
VERSION     DACGLW010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.188"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            complement(<1..244)
                     /locus_tag="IXT53_002920"
     CDS             complement(<1..244)
                     /locus_tag="IXT53_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002384409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HAQ4906673.1"
                     /translation="MEVIIMNVGFIGAGKVGCSFGKYFQEHKIQVTGFYSKSEDSSLA
                     ASNFTSSKQYLNLRELVDENDTIFITTPDGQIQEVWQ"
     gene            482..>1334
                     /locus_tag="IXT53_002921"
     CDS             482..>1334
                     /locus_tag="IXT53_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ4906674.1"
                     /translation="MTKFTIEIMQTLLNKGDLDELFQDHLERAINSLLQAELTAFLDY
                     EKYDRNGFNSGNSRNGNYSRTFKTEYGELNLTIPRDRNGEFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMAEISKLIEKMYGHHYTPQTISNMSKLVAEDVLAFKERTLEANYSVI
                     FMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAPTESAYIWKEVLQDLRKRG
                     LEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIAHKVRVRGRKEICEDFKLV
                     YQANSKEE"
BASE COUNT          474 a          217 c          228 g          415 t
ORIGIN      
        1 cttgccatac ctcctgaatt tgaccatcag gagtcgtaat gaaaatagta tcattttcat
       61 ctactaattc tcttaagttt aaatattgtt tactagatgt aaaattcgaa gctgctaatg
      121 acgagtcttc gcttttacta taaaatcctg ttacttgtat tttatgttcc tggaaatact
      181 tcccgaatga acaacctacc ttgccggctc caataaaacc aacgttcata attatcacct
      241 ccataaagaa gtgagactac aagtcccatt cttacgatat gagcttaaaa ataaaaatta
      301 aatcaaatat acagtatgtt tttttcaaat atcatccata gttaatttat cataatatcc
      361 catattttaa aataaaagat agcgtaaaat attttgtgta aatagaaatt tagggtagaa
      421 aaaaaagtcc attctgtaaa attaaagtta ccacacagaa attttatagg aggactttct
      481 catgactaaa tttactatag aaattatgca aacactactc aataaagggg atttagatga
      541 attatttcag gatcatttag agcgagcgat taattcactt ctacaagcag agctaactgc
      601 ttttttagat tacgaaaaat atgatcgaaa tggattcaat tcaggaaatt cccgtaacgg
      661 gaattattca cgtacattta aaacagaata tggcgagttg aatttaacta ttcctagaga
      721 tcggaatggc gaattttctc aacaaacact accagcatat aagcgaacca atgattcatt
      781 agaaacgacc attatccaat tatttcaaaa aggcattact atggctgaaa tctccaaatt
      841 aattgaaaaa atgtatggtc accactatac gccacaaaca atctccaaca tgagtaaatt
      901 ggtggctgaa gatgttttag cttttaaaga aagaacgtta gaagccaatt attccgttat
      961 atttatggat gcgacgcata tccctgtgaa gcgacagact gtttcgaagg aagcagtgta
     1021 tattacgatt ggtattcgtt tggatggaac caaagaagtt cttgggttta ctattgcccc
     1081 aactgagtct gcttatattt ggaaagaagt tcttcaagat cttagaaaac gtgggttaga
     1141 agaagtttta ttagtagtga cagacggatt aagctgtatt gaagaaagta tccatagtgt
     1201 gtatccgaat gcccaatttc aacaatgttg tgtgcatgta tctagaaata tcgctcataa
     1261 agttcgtgtt cgaggtcgaa aagaaatttg tgaggatttc aaattggttt accaagcgaa
     1321 ttcaaaagaa gagg
//
LOCUS       DACGLW010000178         1312 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.189, whole genome shotgun sequence.
ACCESSION   DACGLW010000178 DACGLW010000000
VERSION     DACGLW010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1312
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.189"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            complement(156..1297)
                     /locus_tag="IXT53_002922"
     CDS             complement(join(156..1069,1069..1297))
                     /locus_tag="IXT53_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HAQ4906675.1"
                     /translation="MAKAKKKFDEDFKKMILDLNQSGQSVEELAAQYGIATQTIYRWK
                     KLHTKNVATGMTEAEILAMKKEMARMQEENTIPKKGFNHIRSKVKMSDVFEFIQSNVH
                     DHDVKQMCTVLEAPKSSYYDWKKKKPSKRQSENKRLNRLISGIYFENKGIYGAPKIHK
                     ILVGRGETLSLKKVQILMRKLGLRSVTLKKYKPGKQAKVKSEGRKNLLNQDFTTTSIN
                     QKWVTDITYIHTLKDGWTYLSTIQDLHTKKVIGWKFGKQMTKELVIETLDNALLNQTP
                     SENLIIHSDLGSQYTSEAYEAKLNELNIRHSFSRKGCPYDNAGIESFHASLKKEEVYP
                     SKAYENFEAAYLALFQYIEGFYNRKRIHSSINYLTPNQMEELALQA"
BASE COUNT          323 a          263 c          278 g          448 t
ORIGIN      
        1 gacacgattt gtgagaattt tttcgagatt ccagcgggtg agtatctcat cgtttcgacc
       61 agcggtcaaa ccaaaaccgt ttgaccgctg atcgaagcga tgaagttgaa accctgtgga
      121 aatctcgcca aaaaaatgta agtggtagct aggaactaag cttgcaatgc caactcctcc
      181 atctgatttg gtgttaaata gttgatacta ctatggattc gcttgcgatt gtaaaatcct
      241 tcaatgtatt gaaataacgc taaataagca gcttcaaagt tttcatacgc ttttgatgga
      301 tagacttctt cttttttgag tgaggcgtga aacgattcga taccggcatt atcgtaggga
      361 cagcccttac gactgaagga gtgccgaatg ttcaattcat tcagctttgc ctcataggct
      421 tcgctagtgt actgagaacc caaatctgag tggatgatca agttctcgga gggtgtttgg
      481 tttaagagcg cattgtcaag ggtttcgata accaattctt tcgtcatctg cttgccaaac
      541 ttccaaccga tgactttctt tgtgtggagg tcttgaatag tggagaggta ggtccagcca
      601 tcttttaggg tatgaatata ggtaatatct gtcacccatt tttgattgat cgacgtagtg
      661 gtaaaatctt ggtttaacag gtttttacgt ccttcacttt taactttcgc ttgtttgcca
      721 ggcttgtact tcttgagggt gactgaacgc aagcctaatt ttctcatcag tatttgtact
      781 ttctttaagg acaaggtctc gccacggcca acaagaatct tatgaatttt aggcgcacca
      841 tagataccct tgttttcaaa gtagattcct gaaatcaagc ggtttaagcg tttgttttct
      901 gattggcgct tggaaggttt ctttttcttc cagtcatagt agctggattt cggtgcttcc
      961 aaaacggtac acatttgttt tacgtcatga tcatgaacat ttgattgaat aaattcgaag
     1021 acatcactca tttttacttt tgagcgaata tggttaaagc cttttttagg atagtgtttt
     1081 cctcctgcat gcgggccatt tcctttttca tcgctaggat ttctgcctca gtcatgccgg
     1141 ttgctacatt ctttgtatga agcttcttcc aacgataaat cgtttgtgta gcgataccat
     1201 attgcgctgc cagttcttcg accgattggc cagactggtt taaatctaag atcatcttct
     1261 taaagtcttc atcaaatttt ttcttggctt ttgccatcgt ggacacgtcc tt
//
LOCUS       DACGLW010000179         1296 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.190, whole genome shotgun sequence.
ACCESSION   DACGLW010000179 DACGLW010000000
VERSION     DACGLW010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1296
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.190"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            46..>1296
                     /locus_tag="IXT53_002923"
     CDS             46..>1296
                     /locus_tag="IXT53_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HAQ4906676.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFK"
BASE COUNT          401 a          258 c          251 g          386 t
ORIGIN      
        1 atcctttaga attaatttgg ctaccaattc ataggaggat tcaagatgtc tcatactcag
       61 cttatcaaag ataccctaaa tattctagac ctaaatatct atttcgagga aaattgttta
      121 actaaagaaa aatacaaagg gcaaatttgc ttgatttaca aaggaactct tctgtacaag
      181 ccagaagagt gttcccattg tctttgtgtc gtcccctcta ggatcattcg ttgggggacg
      241 acaaccgttc ggttgttact gaatgatgtg tctgagtatc gcacttatct ggagctgaag
      301 aaacaacgat ttaaatgtaa gtcttgccaa agaacattcg tagcggatac ctctgttgct
      361 gaaaaacact gtttcattag tcaaaaagtt cgttggtctg tcattgctcg cttaaaagag
      421 aatacttcca tgacggaaat cgctcgtcag aaaaatattt ccacttcttc ggtctatcgt
      481 gtcatgaagc gattctaccg cccattgaat cctttcaaac agactttgcc aaaggtctta
      541 tgctttgatg aattcaaatc ggttcgaagt gttgccagtg caatgagttt tatcatgatg
      601 gacggacaaa gccatgcatt attggatatt gtcgaaaatc gacgcttgcc ttatttagaa
      661 cgctattttt ctcggttttc attagcgact cgtgaagccg ttcaattgat tgttattgat
      721 atgtatgcgc cttatgtttc gttggtgaaa aagcttttcc cgaatgctca gttgatcatc
      781 gatcgtttcc acatcgtcca acatattggc agaactttct tgaatcatcg agtgaaagaa
      841 accacggtac gacttaaagg aaattcccac tctcaacgag ggttagggaa aaaattgaag
      901 cgctattgga aactcctaca aaaagaagaa gcaaagttaa actatgaaaa acgggtttgg
      961 cgtgccagtt ttaaagatta tttgactgaa agtgagattg ccgatcgact gttagctgct
     1021 tgtcccaact tacgccaagg ctatcagtta taccaagaca ttctttatgc ggtaaagaaa
     1081 agagatcagt cactatttga ggattgttta actagagaca ttagcggtct gccagaaacc
     1141 tatacaacca ctttacgaac gtttaaaaag tttctgccac agatccaaaa tgcgcttcac
     1201 tacagctatt caaacggacc attggaatgc ttaaacaacc acatcaaagt attaaaacgc
     1261 aatgcgtacg gctttcgcaa cttttacaat ttcaaa
//
LOCUS       DACGLW010000180         1296 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.191, whole genome shotgun sequence.
ACCESSION   DACGLW010000180 DACGLW010000000
VERSION     DACGLW010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1296
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.191"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            141..398
                     /locus_tag="IXT53_002924"
     CDS             141..398
                     /locus_tag="IXT53_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906677.1"
                     /translation="MSNLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
     gene            complement(630..1043)
                     /locus_tag="IXT53_002925"
     CDS             complement(630..1043)
                     /locus_tag="IXT53_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HAQ4906678.1"
                     /translation="MDQKAFIESIDSTIDRLKKEIRFYNRVIGIGNIIKIVLSASIPI
                     LIDQASEHRSLLLIVSIASAIITIIQSGMSAFNYQDKVQTSTELLMKIEKEKLLYITK
                     TSPYNKTDEENFHLIVTTLQTELNDIISDFNQVNN"
BASE COUNT          490 a          166 c          162 g          478 t
ORIGIN      
        1 ttctgtccac gttcatatta ctccaaagtt ttcctcattc caaaagaaaa gcacagaata
       61 cacaaagaag cgtttttatt tgtataaact atatcaaagt tctatactta aattatcaaa
      121 taaaaaggac gtgaagataa atgtcaaatt tagaaaacaa agaagaaaaa gttgtaaaca
      181 aaattgtttc tgttgtcaat aaactggata aagagttaga cgaacttgat acgttatctg
      241 aaaatccaga aaaaaaacat aacctaaaaa aatggttagt tgaacgaaag gctattcatg
      301 aaattaaaaa agttcttcat gaagctgata aatatgaaaa atatgatgaa aaagaacttg
      361 ataaagaatt taaagaaatc aatgacttgt tactataatt gatttatacg actcattttt
      421 gagtcgtcag actgcaaaca acactctaaa tttaggagtt tgtctacagt ctgaataaat
      481 tttaaattta ttgtccataa tgttattatg aatttatagc attaagaaaa gagataagac
      541 tttacatttt tttaggaatg taaagtctta tctcttttct taattgtaca attagtatta
      601 tctgcactac tttctaatta aataaatttt tagttattaa cttgattaaa atcagagata
      661 atatcattta attcagtttg aagggttgta acgattaaat ggaaattttc ttcatctgtt
      721 ttattatatg gggatgtttt cgttatatat aacaactttt ctttttcaat tttcattaat
      781 aactctgtcg aagtctgtac tttatcttga taattaaatg cagacatccc actttgtata
      841 attgtaatta ttgctgaagc aattgaaaca atcaatagta aagacctgtg ttcggaagcc
      901 tgatcaatta atattgggat actagctgat aagactattt ttataatatt tccgatacca
      961 attactctat tataaaatcg aatttctttt tttaatctat cgattgtaga atcaatagat
     1021 tcgataaacg ctttttgatc catcattttc tctccaatca ttttattaaa atcattaaca
     1081 aaaatattat aataggattt aagtaagttc taactaatca catataaaaa taaaaaaaca
     1141 tttgtatttt atcaaagttt tattatactt gtcaatttgt gtacatccta aaatataagc
     1201 tatttgttta cttctgtaga atatatactt tgttgttaga taagttgcac atgtttatag
     1261 aatctaaata ataaattttt atgtaccgcc cctcaa
//
LOCUS       DACGLW010000181         1246 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.192, whole genome shotgun sequence.
ACCESSION   DACGLW010000181 DACGLW010000000
VERSION     DACGLW010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.192"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            200..742
                     /locus_tag="IXT53_002926"
     CDS             200..742
                     /locus_tag="IXT53_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906679.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            832..>1246
                     /locus_tag="IXT53_002927"
     CDS             832..>1246
                     /locus_tag="IXT53_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HAQ4906680.1"
                     /translation="MDELKRTEIQIIDKKERPKNGFVLSEKTDLENTEAFNKESVKDL
                     RKLPTKDINGKDFTSKDFEKYDLTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIV
                     GVVTDPVDDNGENKEAIEKSKLIHEKTKASYPFLMP"
BASE COUNT          514 a          141 c          238 g          353 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccag
//
LOCUS       DACGLW010000182         1215 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.193, whole genome shotgun sequence.
ACCESSION   DACGLW010000182 DACGLW010000000
VERSION     DACGLW010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.193"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            414..947
                     /locus_tag="IXT53_002928"
     CDS             414..947
                     /locus_tag="IXT53_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HAQ4906681.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT          390 a          225 c          225 g          375 t
ORIGIN      
        1 cttattaccg cagatgcaag cttttgaaca ccgtttattg agaagttgtg aaatcagcgt
       61 cctgacctga gtattagaga aacaaatggc aaaatagttc ctcatatttt gctatttttt
      121 gagctaatac cgagggtcag ctgattgaac accgtttagt ggagaagttg tgaaaaagtc
      181 gcctactttc gagtcgtaag aaattttttt gaaaatggtt cctcacaact ttgcataaat
      241 ttgacttaat accgagaagt catgatcccc atctaactcc aaatcacaag gaatgagttc
      301 aaaaactagc ttttctcaac aaatattaca taaaaaattc tatgtttttt gttaaattta
      361 tttgcttttt tcaaaagtat gttacagtaa tttcccaagg aggttttaca gaaatgaatt
      421 ttgttgccat ggattttgaa actgccaatc atcagcctta tagtgcttgt tctcttgcgc
      481 tagtcatggt aaaaaatagc cagatcgttg atgaattcta tacattgata cagcctgaga
      541 ctcctttttt ctggcgaaat gtccaaattc acggaatcca tcaagaagac gtacgcaatg
      601 cgccgaaatt cccagaagtc tggcaaacta tccaaccata ttttcaaaaa aatcgtttag
      661 tcgttgcaca taatgctgct tttgatacaa aggtcttagc gggatgctta gattattatc
      721 atcttgaaca gcctaattat ctttctttat gtacagtgaa aagtagccgt cgcctgtttc
      781 ctgaatttcc aaatcatcga ttgaacactg tgtgcgaaaa tttgaacatc caattagatc
      841 atcaccatga tgcattggaa gatagtcgtg catgtgcgga aatcctgctt tatcaagaaa
      901 aaaaattcgg gacagatcca ttgaaaaaac ttgtgactat aaagtaaaag aatacttatg
      961 aaacacacaa agaggtcgtg agcctgccgt ttcactccga gtcacaagga gcaatttcaa
     1021 aaaatagctt ctcatgttta ttaaatattg tgactggtga ccgaggagct ggcaggagaa
     1081 caccgtttat tggagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
     1141 acaaaaatag cttctcatat ttttcatttg gttggactta ttaccgcaga tgcaagcttt
     1201 tggacaccgt ttaca
//
LOCUS       DACGLW010000183         1195 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.194, whole genome shotgun sequence.
ACCESSION   DACGLW010000183 DACGLW010000000
VERSION     DACGLW010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.194"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            141..461
                     /locus_tag="IXT53_002929"
     CDS             141..461
                     /locus_tag="IXT53_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906682.1"
                     /translation="MKKRLLWCLLFLSLSLTSQPVFAEETQRSPITGTRLYKNVYVQT
                     TDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            913..1113
                     /locus_tag="IXT53_002930"
     CDS             913..1113
                     /locus_tag="IXT53_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HAQ4906683.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
BASE COUNT          403 a          200 c          225 g          367 t
ORIGIN      
        1 aaaaaagagg ttgtgacgtg gtcacgacct ctttttgacg attatgatac aaaaaacatc
       61 atttatgaac aaaatgtgaa taacgggcgt tcttgagata tgattttaat tgggaaaaac
      121 ttatcgataa ggaggtcttt atgaaaaaaa gactgttatg gtgtttgctg tttctatccc
      181 taagtttaac aagtcagcca gtttttgctg aagaaacaca gcggtctcct attacaggca
      241 cacgtctgta caaaaatgtt tatgtacaga caacagatct tgattctgcg attcctgaaa
      301 cagaagaacg gtctttgcag ccaagatttg gttacttttg gtactgtccg acctgtgatt
      361 atgaaagttg ctggcatttc ttacattcta cagctgcagc ttttgctttt actcatactc
      421 agaaatatga tggacatcat cctgttattt atgaggtata gatatgagca aaaaagaaaa
      481 actaatctcg ttgatcgtat tggtttgcct tgttcttctg gtaactgtca taggctatca
      541 gaaataccat gagaacatca gacctactaa agaatcttcc ccagcctcga ccgttgaaac
      601 agaaccgata agtagtcaga aagaataatt tttttagttt aaagaagaac atcagagcaa
      661 aaaaacacca actgaacaat atgtcgttta ttttaaaaaa agttcggttt ttcactttat
      721 gtaatcgctg agaaaacttt ttttaataaa aaaagtcgaa aaactctagc ttttttgtaa
      781 acggtatgat atactatagg agtagttttt gttcggtaat ctgtggtagc ttgtttcaaa
      841 taaacatctt ccagatatac accattcaag taattgcaaa atatgtgttg atacacaagg
      901 aggattttta ttatggaaac aggtacagta aaatggttta actcagaaaa aggatttgga
      961 ttcatcactg cagaaaacgg aaacgatgta ttcgttcatt tctcagctat ccaaggcgac
     1021 ggattcaaaa ctttagaaga aggccaagca gtaactttcg atgttgaaga aggccaacgc
     1081 ggacctcaag caacaaacgt tactaaagct taattaacct atactaaaaa ataaacgccc
     1141 agacatttgc gtgtctgggc gtttttgtct attttcttta aaaagcgatg agagt
//
LOCUS       DACGLW010000184         1112 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.195, whole genome shotgun sequence.
ACCESSION   DACGLW010000184 DACGLW010000000
VERSION     DACGLW010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1112
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.195"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            154..417
                     /locus_tag="IXT53_002931"
     CDS             154..417
                     /locus_tag="IXT53_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HAQ4906684.1"
                     /translation="MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIG
                     LDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVDEIDWDNL"
     gene            417..689
                     /locus_tag="IXT53_002932"
     CDS             417..689
                     /locus_tag="IXT53_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HAQ4906685.1"
                     /translation="MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQ
                     SFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEIYSARTHYESQ"
     gene            766..1095
                     /locus_tag="IXT53_002933"
     CDS             766..1095
                     /locus_tag="IXT53_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ4906686.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
BASE COUNT          423 a          141 c          220 g          328 t
ORIGIN      
        1 ctctaggaaa aaattaacta gcaccacggg tataaaggaa acggataaca ctttacaatg
       61 ctgttttggt actccaattt aaacaaaaca atcgaattta cgtacttaat taagtacgtt
      121 agaatggaga tataaattag agggggagta gatatggcaa aaacagtctt aaatcctagc
      181 tcagcacgga aaaattttta taagttgttg aaagatgtaa atgagaatca tacggaaatt
      241 gaaatcatta gtgagcgcag tgaaaataat gcggtgctta ttggtttgga tgattggaaa
      301 gcaatcaaag aaacgctatt acttgagcaa ataggcactt tgggtgttgt tcgtaagcga
      361 gaaaaagatg atagtggatt tactaatgtt gatgaaattg attgggataa cttataatga
      421 ataactattc tgtgatgatt aaaaactcgg ctaaagctga tttaaagaaa atcaaacagt
      481 ctaatttgaa acctcaattt gagaaggtta ttcagacttt aaaggaagat ccctatctcc
      541 ctacccaatc atttgagaag ttaaaaccta cacatgaagg taggtattct agaagattga
      601 atagacaaca ccgggttgtt tataaggtag atgaagatga acacgtggtt gaaatttatt
      661 cagcaaggac gcattacgag tcacagtaat tacttgtaaa tacattatct cgtggtataa
      721 tgtatttact aagtaattac atactgttag aaatgaggga tttaaatgat tacaaaaaca
      781 agaaaacaag gcaattcaat catgttgaca gtacctaaag attttaatgt tcctaatgga
      841 gtcgaagttg aagcaaagtt ggtagagaat ggtattcttt atgaatttgt tgaaccacaa
      901 aaagaatttt atgattttag cgaagatatt ttgtctgata taattgcaga aggctacgat
      961 aaagatgaga ttcttgtaga atttaagaat agaaaaaaca aaatgcattc ttcctttaga
     1021 gatattgctg aagatacact tactaattct aaagtaatga ctaaagagga gttggcaaca
     1081 gaaattggct tatgaaatcg ttccttcaaa ac
//
LOCUS       DACGLW010000185         1073 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.196, whole genome shotgun sequence.
ACCESSION   DACGLW010000185 DACGLW010000000
VERSION     DACGLW010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.196"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            2..>1073
                     /locus_tag="IXT53_002934"
     rRNA            2..>1073
                     /locus_tag="IXT53_002934"
                     /product="23S ribosomal RNA"
BASE COUNT          329 a          214 c          308 g          222 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact aggatgttgg cttagaagca
      961 gccaccattt aaagagtgcg taatagctca ctagtcgagt gaccctgcgc cgaaaatgta
     1021 ccggggctaa acatattacc gaagctgtgg agtacacctt taggtgtatt ggt
//
LOCUS       DACGLW010000186         1058 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.197, whole genome shotgun sequence.
ACCESSION   DACGLW010000186 DACGLW010000000
VERSION     DACGLW010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1058)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.197"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            complement(36..992)
                     /locus_tag="IXT53_002935"
     CDS             complement(36..992)
                     /locus_tag="IXT53_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906687.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          344 a          176 c          160 g          378 t
ORIGIN      
        1 tagaaaaagc agtcggactt tctaaaagaa gttcactatt cgatcctgtc gatgatttct
       61 tcaggaaaat cgtctatttg cttgtcaatt ttgctgtctt tgtatttctc ttcttggata
      121 gtcaacaaaa tctttgtaag attcccccag tctactgctc ttggcggatc gagccaaacc
      181 actacttgag atgcactttc ttcatataga ttattcctat tcgtgattat aacatccggg
      241 aactcaggct ttcctgcctc tatcacttcc gtgttcagat taatgtacaa gcttaaattt
      301 ttcttgataa attgattata ggcagcacca taagaaaaat cgatgatgat ctttactggt
      361 tcagaaatca gttgaatggg tatgtgattg atcaataata ataaataact gaaaaaaaga
      421 aaaaatttat cttgaagcaa ctctttgcgt tttgacgtta gatcatttat atactctatc
      481 aaatagcaat aaaatgctgg atagttttct ctaaaaaaag aaacatcaag tggaaattca
      541 tcagctttat taatagtgta atgcaaaagt ttaaaatgta ttttggttaa ctctcttcgg
      601 atcaattggc tcgtctcttc tcgcaacaga ttaaactgat tttcaaaact ttcaatgaat
      661 cgttcattca accattcgat tgtttcatct tcattttttc tcaagtattt ttgatccaat
      721 ttatcatgaa gataaagcca agagagtgtc ttattgataa aaataacgga tttttctcct
      781 aatactcctt ttaagtggta aaatcctata tccgaaaaaa atagcgactg attgctagca
      841 atatctaaag aatcagcttg ttcagccaac aggttgattg ctaaaaagtg gaataatttc
      901 tttttttcat aagcagacag aggataatac tgttttaaat cttttaacat tttttctgtt
      961 tttataatta taggtagcgt atacaattcc acaaaactta tctctgttga gttaaaaaga
     1021 ttatataaaa aaagccgaat gtttttagga ttccctgt
//
LOCUS       DACGLW010000187          945 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.199, whole genome shotgun sequence.
ACCESSION   DACGLW010000187 DACGLW010000000
VERSION     DACGLW010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..945
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.199"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            73..>945
                     /locus_tag="IXT53_002936"
     CDS             73..>945
                     /locus_tag="IXT53_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HAQ4906688.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDEFFIQSVASKRNN"
BASE COUNT          331 a          164 c          175 g          275 t
ORIGIN      
        1 agacaactaa aaaagtcatt tgtgctacac tcaaaatagt tccgaccaaa gaattataag
       61 gagtgagtac aaatgaccta tacccatctt acaacggatg aacttgtaat aatagagtct
      121 tatttcaaaa tgaatcaatc tgttgctaaa actgcccatt gcttgaatcg ttcaagacaa
      181 acgatccata aagtatatct gttcttcaag caaggaaaat cagccttaga atattatcaa
      241 cagtataaga aaaacaaatc aaactgtggt agacgtccgc ttgttttacc cgaggaacaa
      301 tcagaatata ttcaaagaaa ggttgttcaa ggatggacac ccgatgtgat tgttggtcgt
      361 gcagcgtttc ctattagttg ttctgctcgt accatttatc gtatgttcaa aaaggggcta
      421 tttgattctt ctgacttacc gatgaaaggc aagcgtaaac cgaatggaca tcaagaaaga
      481 cgtggaaaac aaactttccg ccgctctatt catgaacgtg aaaaggatta tagccaattc
      541 tcaaatgagt ttggtcacct tgaaggtgac actatcgtag gtctgaaaca taaaagtgct
      601 gtaattacct tagttgaacg attatcaaaa gttatcatca cattgaaacc gtgtggtaga
      661 caagcgattg atattgaaaa aaaattaaat caatggtttg aatctgtacc gaaaaaccta
      721 ttcaaatcca tcacttttga ttgtggaaag gaattttcaa attggaaaca gatcagtaat
      781 gtcaatgata ttgccattta tttcgctgat ccaggaacgc cgtctcaaag aggcctaaac
      841 gagaattcta acggattgtt acgtagagat ggtttattga aatctatgga tttcaattca
      901 gtagatgaat tttttattca atctgtcgca tctaaacgaa ataat
//
LOCUS       DACGLW010000188          928 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.200, whole genome shotgun sequence.
ACCESSION   DACGLW010000188 DACGLW010000000
VERSION     DACGLW010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..928
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.200"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            <1..>928
                     /locus_tag="IXT53_002937"
     CDS             <1..>928
                     /locus_tag="IXT53_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HAQ4906689.1"
                     /translation="VRYKIASLLTEKVSLSFIAKNCQVSLTTVIRTLKEFKSYLPKQS
                     KRILPRVLMVDEFRSHASIEDKMSFICADGETGQLIDVLPTRKLPRLTSYFLGCANPE
                     EVKFLVTDMNAAYFQLTKRILPNAKVVIDRFHIIKHMNQAFNELRIREMNELRKVGQK
                     SQAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLGFSTSLKEA
                     YPYFHELVEAFRDKDPDLFFSLLAELPETLDDGFREKLQNLLTYEEGITNAMIYPYTN
                     GKIEAKNTHIKTMKRVSYGFKSFENMRIRIFLI"
BASE COUNT          310 a          180 c          174 g          264 t
ORIGIN      
        1 gtcagatata aaattgcttc tttactgact gaaaaagtat ctttatcttt tattgcgaaa
       61 aattgtcagg tatctttgac caccgttatt cgtacgttga aagagtttaa aagctactta
      121 ccaaagcaat ctaagaggat tctccccaga gtattgatgg ttgatgaatt tcgttcgcac
      181 gcttccatag aagataaaat gagctttatt tgcgcagatg gcgaaacagg acaattaatt
      241 gatgtcttgc ctacgcgtaa attgcctcga ttaacaagct atttcttagg ctgcgctaat
      301 cctgaagaag taaaattttt agtcacagac atgaacgccg cctacttcca gcttaccaaa
      361 cgtattctgc caaatgcgaa agtagtgatt gatcggtttc atattatcaa acacatgaat
      421 caagccttta atgagttgcg tatccgtgaa atgaatgaac ttcgtaaagt aggacagaaa
      481 agtcaggcag aaaaactgaa aaagaactgg cgctttttgc taaaaaatcg tgcaaacatc
      541 aaccattatg aatacaaaac atggaaaagt tttcgagcac caaaataccc atttcttacg
      601 gaagcgatga tgattgatcg attgcttggc ttttctacgt ccctgaagga ggcgtatccc
      661 tattttcatg aattagttga agcctttcga gacaaagacc ctgacttatt tttctcctta
      721 ttggcagaac ttcccgaaac gttagatgac ggctttcggg aaaagctcca aaaccttctg
      781 acctatgaag aaggcatcac caacgcgatg atctatcctt acaccaatgg aaaaatagaa
      841 gcgaagaata cccatataaa gacaatgaaa cgagtatcct acggatttaa atcatttgag
      901 aacatgagaa ttagaatctt tttgatca
//
LOCUS       DACGLW010000189          874 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.201, whole genome shotgun sequence.
ACCESSION   DACGLW010000189 DACGLW010000000
VERSION     DACGLW010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..874
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.201"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            <1..735
                     /gene="tnpB"
                     /locus_tag="IXT53_002938"
     CDS             <1..735
                     /gene="tnpB"
                     /locus_tag="IXT53_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family element transposase accessory
                     protein TnpB"
                     /protein_id="HAQ4906690.1"
                     /translation="RIKLHRQPKGIIKSATISRHSSGKYYISLLCKEEIIELPKTNST
                     IGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKN
                     YQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSIS
                     DVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCH
                     THHDRDINASINILTEGLRLHSMGLA"
BASE COUNT          323 a          139 c          160 g          252 t
ORIGIN      
        1 agaattaagc ttcatagaca accaaaaggg attatcaaat ctgctacaat atcacgtcat
       61 tctagcggta aatactatat ttctttgttg tgtaaagaag aaattatcga attacctaaa
      121 actaattcca caattggtat tgacttaggt attatggact ttgcgattct ttctgatgga
      181 caaaaaattg ataataataa attcacgtcc aaaatggaaa agaaactaaa gcgtgaacaa
      241 cgcaaattgt ctaggcgtgc tttattggct aaaaataaag gtaccaatct atttgaagct
      301 aaaaattatc aaaaacaaaa acgcaaagta gctagattgc atgaaaaagt aatgaatcaa
      361 cgtactgatt ttctgaataa gttgagtaca gaaatgatca aaaatcacga tattatctgt
      421 attgaagact taaacacaaa aggtatgttg cgaaatcata aattagctaa aagtatttct
      481 gatgtttctt ggtcaaagtt tgtgactaaa ttacaataca aggctgactg gtatggacga
      541 aaaatcatca aagtagataa atggtttccg tctagtcaga tttgttcaga gtgcggacat
      601 aaagatggca agaaatctct tgaaattcga gaatggactt gtcctgtttg tcatactcat
      661 catgatcgtg atatcaatgc tagtatcaat attctgaccg aaggtctaag actacattct
      721 atgggattgg cttagatcca atcatcaaga accgtaggaa ctacggggat agcttggtaa
      781 ataagagaca ccgctgttag taaagaaata cgctatcaag tatgctctat tcccaagaag
      841 ctcccacttc aagcgttaag aaccgtcagg ttta
//
LOCUS       DACGLW010000190          863 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.202, whole genome shotgun sequence.
ACCESSION   DACGLW010000190 DACGLW010000000
VERSION     DACGLW010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..863
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.202"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            <1..861
                     /locus_tag="IXT53_002939"
     CDS             <1..861
                     /locus_tag="IXT53_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HAQ4906691.1"
                     /translation="EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPD
                     KYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKR
                     YKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIIS
                     YSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMS
                     RKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLS
                     PVEYRKQSIK"
BASE COUNT          374 a          112 c          140 g          237 t
ORIGIN      
        1 gagcaagaag aaatcaaggt taaaacaatc cttcagctca aaaaagaatt taaattgaat
       61 ctattacttt caattgcaca attagcgaag tcaacttatt attactgggt aaaaaaatta
      121 gataagccag ataaatatag caagataaag caagaaatta cagcgattgt aaaagaatca
      181 agaaactctt atggttatcg tagagtcact ttagcgttaa agatgaaagg atatacaatc
      241 aatcataaaa cagttagaaa attaatgtcc caaatgggac ttacctgtca aatccgaata
      301 aagcgatata aatcttataa aggaacagta ggaaaaattg ccaagaatgt gttaaaacga
      361 aatttttcag tagatacacc caataaaaaa tgggtgacag acgtcactga attcaagata
      421 aaaggaagaa aaatctatct atctcctata cttgatttat ttaacggaga aataattagt
      481 tatagtatat caactagtcc aacatataaa ctgattgaag aaatgcttca acaagcaatt
      541 aaaaaaaagg gaactgaggg ttcactgatt ctacattcag atcaaggatg gcagtatcag
      601 atgccacaat atcaaaaaaa attaaaagag aataacatta ttcaaagcat gtctagaaaa
      661 ggaaattgct tagataattc tgtaatagaa aatttttttg gtgtgcttaa atcagagttt
      721 ttttatcgag aaaaattccg atcaattgag atatttcaaa gtaaattaaa tgagtatatt
      781 aggtggtata ataacaaaag gataaaacta aagttaaatg gactatctcc agtagaatat
      841 cggaaacagt ccattaaata gtt
//
LOCUS       DACGLW010000191          814 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.203, whole genome shotgun sequence.
ACCESSION   DACGLW010000191 DACGLW010000000
VERSION     DACGLW010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..814
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.203"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            75..>814
                     /locus_tag="IXT53_002940"
     CDS             75..>814
                     /locus_tag="IXT53_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HAQ4906692.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDATYIPLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNELLQDINSRG
                     VQEVLLFITDGLKGMKDTIHQIYPKAKY"
BASE COUNT          299 a          153 c          138 g          224 t
ORIGIN      
        1 tccatacaaa aaaggaagtc ccttctgtag aataaagtta acgacaacca attcacagaa
       61 aagaggactt ccctatgaat gattttacta cagaaattgt gcaaactcta gtcactaaag
      121 gcgatttaaa tgaattattc cgttcgcact tagaaaaagc gataaacaca ctcctacgga
      181 ctgaattaac ggctttttta gattacgaaa aatatgatcg cactggtttt aattcaggta
      241 attcgagaaa cggttcttac tttcgatcaa tcaaaaccga atatggtgaa ttaacattgg
      301 aaatacctag agatcgtaat ggtgagttta aacaacaaac tttaccagcc tacaaaagaa
      361 caaacgatac attggaaacc actattatcc atttattcga aaaaggtgtt acgatgtctg
      421 aaattgctga tttgatcgaa aaaatgtacg gtcatcacta tactccacaa accatgtcca
      481 acatgactaa agttctgact gaagaagtaa atgcctttaa atccagagcc ttaaatgata
      541 agtatgtcgc tatttttatg gacgctactt acattccact aaaacgtcaa accgtatcca
      601 aagaagcgat ttatattgcc attggtatac gagaagacgg cactaaagaa gtactgagtt
      661 atgcgattgc tccaactgaa tcaacatacg tttggaatga gctgctacag gatattaact
      721 ccagaggagt tcaagaagtc ttgcttttta ttacggacgg cttaaaaggc atgaaagata
      781 ctatccatca aatttatcct aaagcaaaat atca
//
LOCUS       DACGLW010000192          791 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.204, whole genome shotgun sequence.
ACCESSION   DACGLW010000192 DACGLW010000000
VERSION     DACGLW010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.204"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            <1..780
                     /locus_tag="IXT53_002941"
     CDS             <1..780
                     /locus_tag="IXT53_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HAQ4906693.1"
                     /translation="DMLDYYYFEKNLSYNYVIHLKTFTGMIFKFANRRYGLKYNPVNG
                     VSLARKPKTNEEIKKEKDGYLSKEEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGE
                     LISLTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKL
                     IKNNKDKYIFISKNGNPISIQAYNQSLRAVSKELNIDKKVSSHMLRHSHISLLTELGI
                     PLKAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
BASE COUNT          338 a          107 c          102 g          244 t
ORIGIN      
        1 gatatgctag attactacta ttttgaaaaa aatttatcat ataattatgt aatacattta
       61 aaaactttta caggaatgat ttttaaattc gccaaccgtc gatatggatt aaaatataat
      121 cccgtaaatg gggtttcttt agcaagaaaa cctaagacaa acgaagaaat aaaaaaagaa
      181 aaagatggtt atttatcaaa agaagaagtt caaatgattg ctagtaaaca aaaaagttca
      241 catcaacagt cacgttattc tttaattact aaatttttgt ttttaactgg actacgttat
      301 ggtgaattga tatctttgac tgaatctgat tatgatggta ataaaattac ggtttctggt
      361 acttatgatt acgagttaaa aattaaaaca acaactaaaa atacaggttc ttatagaacc
      421 attgaattat caagtaatgc taaagaaatc atagatcaat taattgaaga aaacaaatta
      481 attaagaata ataaagataa atacattttc atctctaaaa atggtaaccc gatatctatt
      541 caagcatata atcaatcttt aagagcagta tccaaagaat taaacataga taaaaaagtt
      601 tcttctcaca tgttaaggca ttcccacatt tctctactta cagaattagg aatcccatta
      661 aaagccatta tggatcgtgt aggacatgaa gattcaaaaa caacattaaa gatttatact
      721 catacaacta aaaatatgca aaatcaacta gtagaaaaac taggaaaaat agaaatttaa
      781 attgcccctt t
//
LOCUS       DACGLW010000193          768 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.205, whole genome shotgun sequence.
ACCESSION   DACGLW010000193 DACGLW010000000
VERSION     DACGLW010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..768
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.205"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            complement(248..601)
                     /gene="tnpB"
                     /locus_tag="IXT53_002942"
     CDS             complement(248..601)
                     /gene="tnpB"
                     /locus_tag="IXT53_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HAQ4906694.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
BASE COUNT          222 a          156 c          135 g          255 t
ORIGIN      
        1 cgtccaaata atttgttggt taaataattg accttttcat tcaatagttg gatttcagtt
       61 gtaagattct gaatggtttg ttgttgtgct tgattggttt tatgggcttc ttgaagcaac
      121 tgaagcaacg tatctgtagt ttccaaaatt tttcaccacc ttgaatcgaa ctgattttgt
      181 tacagaatac cacaagaaac gcacaagaaa taggggttca acacattttt tgtgttaacc
      241 ccccagctta aaaaactcct ttttttcctg gcaaaatagc tcgaggttga tcgatactta
      301 atccttctaa caaccatcga acctgttggt tcgttaaggt tcgaacttcg tctttcgtcc
      361 gaggccattt taacttgccg ttttctatcc gcttataaca gagaagaaaa ccatcaccgt
      421 cccaatagag aagtttgaaa cggtcttgac ggttcccgca aaataaaaaa acggcatctt
      481 cgtaaagctc gagttgatac tcttgttgaa taagactggc aagaccatca attccttttc
      541 gcaaatctgt ttttccgcac acaatataaa tatgtttggt ttgaagtaaa tcataaagca
      601 ttggacaaca gttctttcaa aatagcttga agaatataat tgttaatacc gttatagatc
      661 aaaacttcag aaccattctc tcttttgatg cgcatcgcta acttggcagc cgcgggttta
      721 cctttttctg gtgcactctt ttcgtttcct aattggacag gaacaatc
//
LOCUS       DACGLW010000194          696 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.206, whole genome shotgun sequence.
ACCESSION   DACGLW010000194 DACGLW010000000
VERSION     DACGLW010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.206"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            complement(53..628)
                     /locus_tag="IXT53_002943"
     CDS             complement(53..628)
                     /locus_tag="IXT53_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HAQ4906695.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
BASE COUNT          229 a          151 c          125 g          191 t
ORIGIN      
        1 atgaaaaaac agagccagat tagcattatt ttgctaatcc agctctgttt attcagccta
       61 tttttctttt tgaatatatc aagcgttggg gcaacatagt gatgactaat gaaacaacga
      121 cgatagacaa caagcgatca aggtagtctg tacccgcctg taccaagatc acacttgttg
      181 tctgacttaa acctaaccca tgtaatagct gaacgatcat actggatcct gaggaggtga
      241 tcccgccaaa taactgaacg gtaatgatcg aagaaacgat cgttccaggt accgacaaaa
      301 tcaaagcgag taataacagt ttcaagctgt ttttatcttt gccgagatat cttggcaaaa
      361 tcaatccagc aagcaagcct gtgagaattt ggattggcga ataatacaga gaaaaaatat
      421 ctgtagtcac tccacttaaa ataccgctgg acaatcctgt cagcattcca ggtacaggcc
      481 caagcaatgc accagcaaaa atcgttccga tagaatcgag atagattggc aaacgaagcc
      541 ataaagcaat cgttcctcca atataattga tcccaataca caaagccatc actgtaagta
      601 cgcgagttgc cattttgttt ttattcattt tgttagccca tctttaacct ttccaaatct
      661 ttcataatcg tttcttcttg tgcatttaaa agaacg
//
LOCUS       DACGLW010000195          680 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.207, whole genome shotgun sequence.
ACCESSION   DACGLW010000195 DACGLW010000000
VERSION     DACGLW010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..680
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.207"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
BASE COUNT          241 a          114 c           80 g          245 t
ORIGIN      
        1 ccaaattact tcatcaaaac attgatataa tttttattat atcagtattt attcttgata
       61 cataaatgag aaaataaata ctttaaatct attgactaca agtagtcatt aagtcttcat
      121 aagatgtaca atatgacatt aaaaaaataa aagcttacga ccatccgtta aatctgacac
      181 taaataatgg actgctttct tttgtattag caggttacgt tgactatgaa tattcaccag
      241 attatttgga gaaacctctt caacacgaga acaatgatat taattttaga tttgtctacg
      301 agtcaaaaag taaaacatgg ttagttgatc aattaagcac gataaacaca agcttccatg
      361 aattacctaa acctgaaaat gaagttgaga tggtcattaa aatcttctaa aaaagaagcg
      421 ttgaagggga aatattcctt tcaacgctct tttttatatg cttagaaaca taaataaatt
      481 gtatactttc tttttagccc ctattacttt tcacgaaaaa tatctttttt tcgacgctag
      541 cgtttctcta cttctaatac tgtacacagt ttttgtatta ttgaaaaatt actcattatt
      601 tagcattaat ccacatcgtt ttatcaatcg accttcatac taaactactt taaagtcatc
      661 ttccttctcg tttttcatta
//
LOCUS       DACGLW010000196          634 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.208, whole genome shotgun sequence.
ACCESSION   DACGLW010000196 DACGLW010000000
VERSION     DACGLW010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.208"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            complement(20..91)
                     /locus_tag="IXT53_002944"
     tRNA            complement(20..91)
                     /locus_tag="IXT53_002944"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(56..58),aa:Glu,seq:ttc)
     gene            complement(113..200)
                     /locus_tag="IXT53_002945"
     tRNA            complement(113..200)
                     /locus_tag="IXT53_002945"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(164..166),aa:Ser,seq:gct)
     gene            complement(225..298)
                     /locus_tag="IXT53_002946"
     tRNA            complement(225..298)
                     /locus_tag="IXT53_002946"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(262..264),aa:Ile,seq:gat)
     gene            complement(312..382)
                     /locus_tag="IXT53_002947"
     tRNA            complement(312..382)
                     /locus_tag="IXT53_002947"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(348..350),aa:Gly,seq:tcc)
     gene            complement(384..459)
                     /locus_tag="IXT53_002948"
     tRNA            complement(384..459)
                     /locus_tag="IXT53_002948"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(424..426),aa:Asp,seq:gtc)
     gene            complement(478..551)
                     /locus_tag="IXT53_002949"
     tRNA            complement(478..551)
                     /locus_tag="IXT53_002949"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(515..517),aa:Met,seq:cat)
BASE COUNT          173 a          171 c          151 g          139 t
ORIGIN      
        1 taaaactata ttaaattaat gacccgtacg ggattcgaac ccgtgttacc gccgtgaaag
       61 ggcggtgtct taaccgcttg accaacgggc caggtaaaac aaaatattga aacggagaag
      121 gagggatttg aaccctcgcg ccggtttccc gacctacacc cttagcaggg gcgcctcttc
      181 agccacttga gtacttcccc aaaaccaata agattttgta ctaatgggcc taaatggact
      241 cgaaccatcg acctcacgct tatcaggcgt gcgctctaac cagctgagct ataggcccat
      301 aaaaaactaa aagcgggtga cgagaatcga actcgcgaca acagcttgga aggctgtggt
      361 tttaccacta aactacaccc gcatggcggt tccgacggga atcgaacccg cgatctcctg
      421 cgtgacaggc aggcatgtta acccctacac cacggaacca gtgtttttag tttttaattg
      481 cgggagcagg atttgaacct acgaccttcg ggttatgagc ccgacgagct accagactgc
      541 tccatcccgc gataatatga aaaaggagga taagggattc gaacccttgc acggttttac
      601 ccgcctgacg gttttcaaga ccgttccctt cagc
//
LOCUS       DACGLW010000197          625 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.209, whole genome shotgun sequence.
ACCESSION   DACGLW010000197 DACGLW010000000
VERSION     DACGLW010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.209"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            complement(109..>625)
                     /locus_tag="IXT53_002950"
     CDS             complement(109..>625)
                     /locus_tag="IXT53_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HAQ4906696.1"
                     /translation="IVTDLTYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKE
                     AFGRIPYSLTDVKLFHTDRGKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYK
                     SVKVEFVHQYQFETLAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDN
                     ERGSDTSGEAA"
BASE COUNT          183 a          144 c          115 g          183 t
ORIGIN      
        1 gacaaaaatt ataaggtgtt gtgaatgctg cggtgttatg gctacgattg cccttgacaa
       61 tgagcatcct cggatctgat tttctccggc agaagcacaa tcattcaatt acgctgcctc
      121 tccagaggta tcagatccgc gctcattatc aaggattcgc ttcgccaatc tctgttgtcg
      181 aatttggatc ggtgtttcat acgccaacgt gccatgtaag cgtagatagt tccaccaatg
      241 cacataatca aacaattcta gacgtagctg tgccagtgtc tcaaattggt attgatgcac
      301 gaattctact ttgacagatt tataggttga ttccacaacg gcattatcat acggacaacc
      361 cttcctactc aatgaacgag taattccaaa accattcaga atctcatgaa tggtttggtt
      421 atcaaattcc tttccccggt ctgtatgaaa aagcttgacg tctgttaaag aatacggtat
      481 ccgtccaaag gcttctttta ccaatgaggc atctttcttc tcaccacagg aataaccaat
      541 aatttctcga ttaaacaaat caagtattaa gcagatataa tgccactttt tccccacgcg
      601 aacataagta agatccgtaa cgatg
//
LOCUS       DACGLW010000198          601 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.210, whole genome shotgun sequence.
ACCESSION   DACGLW010000198 DACGLW010000000
VERSION     DACGLW010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..601
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.210"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            <1..323
                     /locus_tag="IXT53_002951"
     CDS             <1..323
                     /locus_tag="IXT53_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HAQ4906697.1"
                     /translation="EALDHIDFMTRKWKKQYPRVVNLLLNPALLTFYNFPHAIRRTIY
                     STNLIEGFNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRIHKGFKEIQDTL
                     ESMI"
BASE COUNT          214 a          106 c           75 g          206 t
ORIGIN      
        1 aagaggcatt ggatcacatc gactttatga ctaggaaatg gaaaaagcag tatccaagag
       61 tcgtcaattt actcttgaat cctgccctat taacctttta taatttccct cacgccatca
      121 gacgaacaat ttattcgacg aacctgattg aaggctttaa taagcagcta aaacgatata
      181 ctcgaagaaa agaacaattc cctaatgaag aatctctaga gagattcttc atttctcaat
      241 ttaatcaata taaccaaaaa tttttaggta gaattcacaa gggatttaaa gaaattcagg
      301 atacattaga gtcgatgatt taactgtaat taacagaatg gattttccat ttacatataa
      361 ttcttgacgc taccataatc taaataacca tcatattcat ctactactta tataattgag
      421 ttttattaaa ctttttaagt ttctattcta tcaaatattt aaacaatctc tgcatccgaa
      481 aagactacct cgtatagttt tactaacaat atttttatta gcgtgttcaa atcctgattt
      541 cttgtatcaa ttattttctg ttcactttca ataagaaaaa agtctttact tccatggata
      601 a
//
LOCUS       DACGLW010000199          528 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.211, whole genome shotgun sequence.
ACCESSION   DACGLW010000199 DACGLW010000000
VERSION     DACGLW010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.211"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            complement(5..90)
                     /locus_tag="IXT53_002952"
     tRNA            complement(5..90)
                     /locus_tag="IXT53_002952"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(54..56),aa:Leu,seq:taa)
     gene            complement(107..178)
                     /locus_tag="IXT53_002953"
     tRNA            complement(107..178)
                     /locus_tag="IXT53_002953"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(144..146),aa:Gly,seq:gcc)
     gene            complement(190..262)
                     /locus_tag="IXT53_002954"
     tRNA            complement(190..262)
                     /locus_tag="IXT53_002954"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(227..229),aa:Thr,seq:tgt)
     gene            complement(280..361)
                     /locus_tag="IXT53_002955"
     tRNA            complement(280..361)
                     /locus_tag="IXT53_002955"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(325..327),aa:Leu,seq:tag)
     gene            complement(369..441)
                     /locus_tag="IXT53_002956"
     tRNA            complement(369..441)
                     /locus_tag="IXT53_002956"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(406..408),aa:Lys,seq:ttt)
     gene            complement(445..517)
                     /locus_tag="IXT53_002957"
     tRNA            complement(445..517)
                     /locus_tag="IXT53_002957"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(482..484),aa:Val,seq:tac)
BASE COUNT          135 a          152 c          128 g          113 t
ORIGIN      
        1 gaaatgccga ggaccggaat cgaaccggta cggtgatcac tcaccgcagg attttaagtc
       61 ctgtgcgtct gccagttccg ccaccccggc gcgattgctt tggcaaagcg gaaaacgggg
      121 ttcgaacccg cgacccccac cttggcaagg tggtgctcta ccactgagct atttccgcgt
      181 gtttaaagat gccggctaaa ggacttgaac cctcgaccct ctgattacaa atcagatgct
      241 ctaccaactg agctaagccg gcatgtaaaa cttaaattat gcgggtgaag ggacttgaac
      301 ccccacgccg taaggcgcta gatcctaaat ctagtgcgtc tgccaattcc gccacacccg
      361 caaaaatatg agccgtacag ggctcgaacc tgtgaccctc tgattaaaag tcagatgctc
      421 taccaactga gctaacggct catatggagg ttaacgggat cgaaccgctg accctctgct
      481 tgtaaggcag atgctctccc agctgagcta aacctccatg gataactt
//
LOCUS       DACGLW010000200          517 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.212, whole genome shotgun sequence.
ACCESSION   DACGLW010000200 DACGLW010000000
VERSION     DACGLW010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.212"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            57..>517
                     /locus_tag="IXT53_002958"
     CDS             57..>517
                     /locus_tag="IXT53_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HAQ4906698.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYS"
BASE COUNT          191 a           72 c          105 g          149 t
ORIGIN      
        1 ataaatatgt tataatagta tcaaatgaat attgctaaaa gaaaagaggt aggaacgtgg
       61 tagcgaagaa atatgtaatc ggtaatttta aaggtggcgt aggtaaaagt acgtgcgcgc
      121 aaatgttagg gtttgaatca gcagttaatg aaggaaaaag aaccttgata attgacttgg
      181 atatgcaagg aaatacttct gatgtgatga acttaacgca tatgaacttc actaacgaag
      241 aaggaggcgg agaaggtgaa ccgattgttt acgaaaatac tgtgactgac gtgttgataa
      301 acaatcttcc acctaaagag gctgtctata aagtaatcaa taacctctat atacttccag
      361 cagacatgtc ctttgaaatg tatgatgatt ggatcaaaga ccaatataat gattcattat
      421 cccaattcaa atatatgaaa gaaaagctag aaccattatt tgatgagttt gacgtcatct
      481 atttagatgt cccaccttct atttctgtat atagtaa
//
LOCUS       DACGLW010000201          509 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.213, whole genome shotgun sequence.
ACCESSION   DACGLW010000201 DACGLW010000000
VERSION     DACGLW010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.213"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            complement(<1..132)
                     /locus_tag="IXT53_002959"
     CDS             complement(<1..132)
                     /locus_tag="IXT53_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HAQ4906699.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          183 a           93 c           91 g          142 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaatagcaa attcccgttg gatcctttcc atacatccat tgtactctga
      421 gaaatcacac aatatcgaaa caaattccgt taggaatgtg ttattcaata aattttataa
      481 tctagaaaca ttttgaataa aaactatta
//
LOCUS       DACGLW010000202          507 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.denovo.214, whole genome shotgun sequence.
ACCESSION   DACGLW010000202 DACGLW010000000
VERSION     DACGLW010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.denovo.214"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            <1..>507
                     /locus_tag="IXT53_002960"
     CDS             <1..>507
                     /locus_tag="IXT53_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906700.1"
                     /translation="QDIEERKNAMHGQAKSAEELINQKRHEAKYTPEAMNREEIMLRK
                     RVEGRTGANIDGKEALTKERIQEAKNSNTGLEKLVKENPQNSTSSHGGRSVDVRENIQ
                     GSLNGHVGRNVDVRENLQSSSNAQGTKTIDVRENLQSSSTSQPKTVDVRENVQNSTSS
                     QGFKSVDVR"
BASE COUNT          205 a           74 c          115 g          113 t
ORIGIN      
        1 caagatattg aagaacgtaa aaatgctatg cacggtcaag ctaagtctgc tgaagaatta
       61 attaatcaga agcgtcatga agcgaaatac acgcctgaag caatgaatcg cgaggaaata
      121 atgttaagga aacgtgtaga agggcggaca ggcgctaata ttgacggaaa agaagcgttg
      181 accaaagaaa gaattcaaga agctaaaaat tcaaatacag gattagaaaa attagtcaaa
      241 gaaaatccac aaaattctac aagtagtcat ggtggccgat cagtagatgt ccgagaaaat
      301 attcaaggtt cactgaatgg tcatgttgga cggaatgttg atgtaagaga gaatttacaa
      361 agttcttcaa atgctcaagg tactaagaca attgatgttc gagaaaatct gcaaagtagc
      421 agtacaagtc aaccaaaaac agtagatgtt cgagaaaatg tacaaaattc tacaagtagt
      481 caagggttta aatcagtgga tgtccga
//
LOCUS       DACGLW010000203        20985 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.guided.047, whole genome shotgun sequence.
ACCESSION   DACGLW010000203 DACGLW010000000
VERSION     DACGLW010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20985
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.guided.047"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            complement(134..577)
                     /locus_tag="IXT53_002961"
     CDS             complement(134..577)
                     /locus_tag="IXT53_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HAQ4906701.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(795..2135)
                     /locus_tag="IXT53_002962"
     CDS             complement(795..2135)
                     /locus_tag="IXT53_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ4906702.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2404..3300
                     /locus_tag="IXT53_002963"
     CDS             2404..3300
                     /locus_tag="IXT53_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HAQ4906703.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(3450..4391)
                     /locus_tag="IXT53_002964"
     CDS             complement(3450..4391)
                     /locus_tag="IXT53_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HAQ4906704.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4517..5638
                     /locus_tag="IXT53_002965"
     CDS             4517..5638
                     /locus_tag="IXT53_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HAQ4906705.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            5896..7398
                     /locus_tag="IXT53_002966"
     CDS             5896..7398
                     /locus_tag="IXT53_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HAQ4906706.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            7465..7992
                     /locus_tag="IXT53_002967"
     CDS             7465..7992
                     /locus_tag="IXT53_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HAQ4906707.1"
                     /translation="MLYVTRHGETTWNAQGLVCGRADVPLTEKGQMQAQKLAEKVVDL
                     PVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYDGLPSKDENFQKAR
                     LAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIRVINAYFHPMPNEG
                     FFTFMVDNTELVSYE"
     gene            8203..9264
                     /locus_tag="IXT53_002968"
     CDS             8203..9264
                     /locus_tag="IXT53_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HAQ4906708.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            9267..10163
                     /gene="murQ"
                     /locus_tag="IXT53_002969"
     CDS             9267..10163
                     /gene="murQ"
                     /locus_tag="IXT53_002969"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HAQ4906709.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            10177..12159
                     /locus_tag="IXT53_002970"
     CDS             10177..12159
                     /locus_tag="IXT53_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ4906710.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(12224..12670)
                     /gene="nrdI"
                     /locus_tag="IXT53_002971"
     CDS             complement(12224..12670)
                     /gene="nrdI"
                     /locus_tag="IXT53_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HAQ4906711.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            12881..14305
                     /locus_tag="IXT53_002972"
     CDS             12881..14305
                     /locus_tag="IXT53_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HAQ4906712.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(14486..15259)
                     /locus_tag="IXT53_002973"
     CDS             complement(14486..15259)
                     /locus_tag="IXT53_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HAQ4906713.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(15256..16161)
                     /locus_tag="IXT53_002974"
     CDS             complement(15256..16161)
                     /locus_tag="IXT53_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HAQ4906714.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(16315..16635)
                     /locus_tag="IXT53_002975"
     CDS             complement(16315..16635)
                     /locus_tag="IXT53_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HAQ4906715.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            17109..17795
                     /locus_tag="IXT53_002976"
     CDS             17109..17795
                     /locus_tag="IXT53_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HAQ4906716.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            17797..18741
                     /locus_tag="IXT53_002977"
     CDS             17797..18741
                     /locus_tag="IXT53_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HAQ4906717.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            18940..19584
                     /gene="pcp"
                     /locus_tag="IXT53_002978"
     CDS             18940..19584
                     /gene="pcp"
                     /locus_tag="IXT53_002978"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HAQ4906718.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            19663..20940
                     /locus_tag="IXT53_002979"
     CDS             19663..20940
                     /locus_tag="IXT53_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906719.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
BASE COUNT         6778 a         3784 c         4362 g         6061 t
ORIGIN      
        1 ctgttcttat tacttgatgc agggcagctt tttcacaacc ccttattcac ggcgttccag
       61 aagtagcttc ttcttatttc ttgaagctga taacttctgg aacatcctct ttttttattt
      121 ggacggataa tggtcattcc atttctttga tcattttttt cattttgtcg atctcttctt
      181 cttgtgcctg aatgatttct tccgctaatt tttttacttc aggatctttt aaatcagctc
      241 gtttacttgc caggatagcg atagaatgat gaggaatcat ggccttcatc caagctgtgt
      301 cgttgacagt agtttggctt cgtaccataa acaaggaacc ggcaaaaaca agaagactag
      361 cggctaagat cataacattt atctttttgt tttcatacat tttccacatg aacaacagca
      421 tgatgactgc catgactgca cccatcatca acgccatgaa tactcgtgta cgactgaaga
      481 agatatgatc gagcgcatag acattcaagt acatcatacc aaacataact accgttgaaa
      541 caccgatcat caacaaaaac ttccaatact ttttcattta aaacaaactc ctttttttca
      601 ttcctttctt ctatcatgtc atgagcatgg agaacaatca attattttgc acagagggtt
      661 tttgaacacc gtttaccaag aggtcatgag actgtttatt cggaaaaagg agttgcaaca
      721 agacttttgt cacaactcct ttcttcaata gctccatcta ttccacataa ataaaataaa
      781 attttctatc gatttcattc attcgtcaat acgaacgcat cattcttttt ttgataactg
      841 aaataaatga acaggtaaag caggccagtc aatacagtta ccccaatgga gttcgtgaac
      901 aggataccaa ggaaaatcaa tcctaagaca gctaatgcaa aaaagctgat tcctttatcc
      961 cataatgttc cttttcttag tgccagtaca aaggatgccg cactgataat gatccagatc
     1021 aataatactg tatacgaaag ggaacctgcc agatagccaa ataatttgtc accgacaaaa
     1081 taagaaagag ctacaccaag ataaagaaca ctggtacaaa ataaaacagc acgttgcggt
     1141 acttgatgct tgtttaaaac agctagtttt ctagaggaac cttttcgatc cttcaaacgg
     1201 aagaacaaaa ttcttgaaga tgcatacaca cccgaattga tagatgagaa caaagcaagg
     1261 acaatcacaa aattgacgat atccgctgca aatgggatat tcattttttc aaataccatc
     1321 acaaaaggac tgacattcgt gcctgctaaa gtattccaag gaaaaagaat caaaagcaaa
     1381 aacaaaggaa tgatgtaaaa ggaaatgatt cttcccatga cacctttgat tgctttagga
     1441 atcgcacgtt taggatcatc tgcttcgctt accgtgatag caatcagttc agaaccgccg
     1501 taagaataga taactacaag caaagaactg atgattccct tcataccatg aggagtaaac
     1561 ccaccatggt cagtcaattg ctgaagggta gggaaaacgc ctgtgcccaa catttgctga
     1621 ccgacaagat aagtagcaaa aatgatcagt aagataatga cactgatttt agcaaaagcc
     1681 agccaatatt ctgtttctgc gaataaccgt accgaataaa gattgatgat cgtagttaaa
     1741 acagccaaca taaaaacaaa tacccaggct ggaatgtgcg ggaaccacaa ctgcaaaaag
     1801 gaagcagctg caacagcttc agcgatgata ttgataatcc acatagacca gtagacccaa
     1861 tcagtaaaat cagcagaatg agcacctaga taagggcgta tcaaaccaga taatccgtga
     1921 tgcgtgtcgc caccgcttaa aacgattttt cccagacctt tcatcacgat atataaaatc
     1981 agcccaccaa tcaaaaaagc aatcaataca gaagggccgg cgatcccaat cgcttcgcca
     2041 cttcctttaa atagacctgc accgattgct ccacctaaag ctaacatggt gatatgtctg
     2101 gagctaagtt gttttttcaa ttttttatct tccataaatg acctctttct tttaaattca
     2161 caatcaaaat ttatattaag aataaatagc aatatttaac aatgactaat gataacaaat
     2221 tttcagacaa ttacaactat tcagaaaact tttaggcgaa ttttaataga agaatgattt
     2281 atttcatcat attttcatac aaatgttaaa ttttcataca aatataggaa acgttttact
     2341 ggctttttac tatttaaaca ggctatcata ggaaaaagtg acagagaaag tgaggcggaa
     2401 aagatgaaca aaaatgacgt actgctagtt gcaaaaaata aatcacttta ttttttgcct
     2461 tccatccgct ctttagatgt ttggtccgga aaagatttac tacagtcgga attcattaaa
     2521 tcagctagac aacagttggc aaattatcag ctgatcgttt ttttagatta tggatttgat
     2581 atggagatgg ctcgacgcgt gcgcccattt accaatgcaa aaattgtgtt atttttttgg
     2641 aatcacttca aagcagaaca cgaggcgatg ctgaagcagg cacaagatga accggcaatc
     2701 gatgagattt atcattttga ttttttggaa gcaaaagaac ttgggctgaa acataatagt
     2761 tcattttatt cgaaacatat gaatttttct tctagcgaac caaagacgga tctatttttt
     2821 ggtgcaacga ataatgggag aaaagaacga gcagaaagct ataagcagga atttataaaa
     2881 agagatgtta cgaccaacta tttcattctt ccacatcgag ggaatgaaca acctggatat
     2941 cttacttatg aagagtacct gaaaaaaaca ggggaatcgc gcgggattct ggaactgttg
     3001 cgggagggtc agcaaggagt gactttgcgg acatttgaat ccatgtattt tcaaaagaaa
     3061 ctagtgacgg ataatcaagc gattgctcat tatcattttt atcgtcctga aaatattttt
     3121 ttgcttcgag aacgtgattt agatgaatta ccggaatttc tgcatacacc atttaagcca
     3181 atcctatcag agctattgga attttatgat gcagaaaatt gggcaaaacg atttatcact
     3241 aacgattcca ctatttatga gcaatacgaa tatgacctct cctctgttga aaacaaataa
     3301 aaaacctaca ttttgaagag attatgtact gaaacaaaca agcacaattc ttatggcatc
     3361 atgagagttg tgttttcttt ttgatctttt attgggctta aaaaaagaga accagctgct
     3421 gattcagctg attctcatca gagagccttt tattctgcgc caaggattcc ttcttttgca
     3481 tggactaatt gggtcaaaaa ggcacaatat ggtgtagcaa tatcatattt ttgtcctttt
     3541 cgccaaatgg caccattgat gtagtcgatt tcagtcaaac gatggttttt gatcaggtct
     3601 tggtacatag atgggtaatg caacccgatg ttgtcaggat cataagtaga agcaatatgt
     3661 tggtacactt cttcttgatc tagtatgatt ccttcttttg ctgcgataga agcaaattca
     3721 gatacgattg ttcgaaccat gctctctgcc gctttttgcg cgcctagttc agcaatatta
     3781 caatccaaaa tcgtgcataa accattcaat gttccattta cacaagcttt tcgccaaatg
     3841 gaataacgaa cgttatggct atagatcgga tttaaatttg cttcttgaaa gacttctaca
     3901 actttttttg taaatgcttc gccttctggt tccaagttct ctagttcgat ttctccatca
     3961 cctaacaatg tgacttttcc gggaccagct agaccagctg tccacatcgt gatcccaaat
     4021 aagatatttt tctttggcac atatttttcc agtacatctt catgacctaa tccatttagt
     4081 aaacacaaaa cataagtatt gtcagtaata atggattgga tcgaacaaaa catatcatct
     4141 aattgattgg cttttgttaa tgctacgatc aagtccacgt gttcgttgct ttcgatgatt
     4201 tcttctggtg aatagattgg cagttttgcg accacttctt taccgttaaa gtcagcaatc
     4261 aacccatttg tacgaatggc ttcaatgtga gcaggccaac gatcgatcaa gaggacctca
     4321 tttccgcttt gatgtagcat caatccgatt cggctgccca ttgctccggc cccagctatt
     4381 gcgattttca tgttttttct ctcctttatt caatgagttc ccttttagta tatagaaaaa
     4441 tggaagaaaa tagcattgtt ttgtttgaaa aaaatgaaaa aaagtgtctt acttaagaag
     4501 aaagtgaggg atacagatgg atattttggc ggcaattgat tcatttaaag gaagtgcttc
     4561 ttcatttgaa ctaaatcagg cagttctaaa tggctgggaa gatcgtaaag gaagaaaaat
     4621 caatgtgccg attgcagatg gcggagaaaa tactgcagaa gcaatctatt acgcgctcag
     4681 gggaacatgg cgtaccattg atgggatgga tctcttattc cgtcagcgaa aaatcagcta
     4741 tttattgaca acttggcaag gaaaaaacac cgcagtcatc gaatctgcac aagcattggg
     4801 gttagatcta cttgataaac caacggatca aactatccga cttgcttcca gttatgcctt
     4861 aggagaactt attaaagatg cactgcttca agaagtagaa caaattattg tcactttagg
     4921 aggaagcggg tgtagcgatg gtggtctagg attactccaa tccttgggtg cttctttaga
     4981 agggtacaca gaaggaaatc ctttattatc tactaaaaaa cttcattttg atcgaagaaa
     5041 cgttcttgtt tctaaagtaa aattgattat cgctgcagat gttaccaatc catatacagg
     5101 tcaacaagga gctgcttggc aatttggaag acaaaaagga ggaatagata cgacgctagc
     5161 tttcttagat aaacaagcag gaaagattgc tcagcaaata aaagatttct gggaggtgga
     5221 tttagatgat attccaggaa gtggagcagc aggcggtatt ggtggagccc tttttttgtt
     5281 gggagcgaag atggtttctg gtttcgattt agtcagccaa atcactggat tggaaaaaaa
     5341 gatcgaacaa gcagatgtca tcattacagg agaagggcgg atcgatcagc aaacgatcca
     5401 cggaaaagtt ccatacggtg tagcagtgct tgcaaaaaaa tatcgaaaac caataatcgc
     5461 tatttgcgga agtagagacc aagagctaga aggactttct gattttctcc caattgtctt
     5521 aagtattcat ttgggtccaa taggtttaga agaagcaatg gaacacaggc aaacattaga
     5581 aaaaacaaaa atcttaggac agacacttag cagatttttc accatcctcc cgaagtagtt
     5641 gacaaaatca ttttctttat gctatcgtaa tttcaaatat aggacaaagt gagggatccg
     5701 tgcattgaaa aattaagtcg attgatgaaa gctgcattca gactgtacct tgtggagaca
     5761 aggaagcagt gtgcagattt tcgggattgg cttggttttt tcgatgtatg ggtctttttg
     5821 tgtgtatcca acccttgcat cctctttagc cagttctgaa aataggactg gtcttttttt
     5881 acgaggtgat ggaaaatgtc taaaatcgaa ataaaaaatc tgacattcgg ctacgacagc
     5941 caaggcacat tattatttga acaagcaaat ctaaattttg acacacaatg gaaactagga
     6001 cttatcggac gaaacggtcg aggaaagaca actttactga atattctaca aaacaaacta
     6061 ccttatcaag ggcaagtaat ccatcagcaa gaatttgcct atttcccgca acagacaaaa
     6121 gataaagaac gtttaaccta ttacgtgtta aatgatatta cggattttga gatatgggaa
     6181 atcgaaagag agctccaatt gatgcaaaca gatcctgaaa tcttatggag agaattcagc
     6241 acactatcgg ggggagagaa gacaaaagtc ctactggcac ttttatttgt ggatgacact
     6301 catttcccgt taatcgatga accaacgaat catttggata tctctggtag aaaacaagta
     6361 gcggcttatt tgaaaaagaa aaaacaaggc ttcatcgtgg tcagccatga ccggggattt
     6421 atcgatgaag tagtggacca tgttttagca atcgaaaaaa gtcaactgga actttatcaa
     6481 gggaatttct ctatctatga agaacagaaa aaacttcgtg atgaatttga aatggctcaa
     6541 aatgaaaaat tgaaaaaaga agtcagtagg ctaaagaaaa cagcagctga aaaagccgaa
     6601 tggtctcgtt cccgagaagg agataaaaca aagaaacaag tcggattcat cgatactgaa
     6661 tctagacgag tgaataaagg agcagtgggt gctgatgctg cacggacgat gaaacgatcc
     6721 aaagcaatcg tgaatcggat ggagacccag atcagcgaga aagaaaaact attaaaagat
     6781 atcgaatata tcgattcttt gacgatgaat agccaagcgt ctcaccataa gcgactttta
     6841 agcgtagaag atcttcaatt agggtatgaa aatctgttat tcgagccaat tcattttaca
     6901 atcgagcctc atcagcgggt ggcgatttca ggtcctaacg gtgcaggaaa gtcatccatt
     6961 atccattatc ttctgggggc attcaacggc aaggttatag gagaaaaata ccagccaaaa
     7021 catctgagca ttagttatgc aagccaaaat tatgaagaca atcgaggaac gttggcggaa
     7081 tttgcagaga aaaaccaagt agactaccaa gcatttttga acaacctccg aaagcttggg
     7141 atggaaagag atgtttttca taacaagatc gagcagatga gtatgggcca acggaaaaaa
     7201 gtggaattgg ctaaatcttt atcacagcca gctgaactat atacatggga tgaaccattg
     7261 aattatttgg atgtcttcaa tcaagaacaa ttagaacaac tgatcttgaa cgtgaaacct
     7321 gccatgttac tagtggaaca tgatcaaacc ttcctggata aagtatctac tgagattatt
     7381 tctcttgaga gaatctaacc cttgtaatct caagaacaac aaggtatggt taaggggaac
     7441 tttgccaacg aaggagagaa tcacatgctt tatgtaacga gacacggaga aacaacttgg
     7501 aacgcacaag gattagtttg cggacgggca gatgtgccgc tgactgaaaa aggacagatg
     7561 caggcacaga aactagcaga aaaggtagta gatttgcctg ttccaatcac gaaaatcatc
     7621 cattcccctc ttcaacgagc aagagataca gcacaagcag ttgctgatcg tctcagcttg
     7681 ccgctgaccg ttgatgaacg attggtagaa atggattttg gggattatga tggactgcca
     7741 agcaaagatg agaactttca aaaagcacgt ttagcgttcg ctgtacgatt cccaaatggg
     7801 gagtcagtct tagatgtgta tgcacggatc gttcctttat tgaaagaatg catcgaagac
     7861 gaagaaaatg tctatctgtt agtttgccat aatgccttga taagagtgat caatgcttat
     7921 tttcatccga tgcctaatga ggggttcttt acattcatgg tagataatac agaattggtt
     7981 tcttatgagt aattatgttt ttgtgttatt taaataaaat atttcaaggc actgcttttg
     8041 cttagcagtg ccttttttag gttatgacaa ttcttttgat aatataaact caacctttag
     8101 aaatgaaatt taattatttt aataaaagta ttgaaatttt gtttctttag atatatactc
     8161 tagtcataga aatgaagacg gatacagaaa aagagggata atatgtttgg cttttcaata
     8221 tttatgaacg attctttgtc agaagagaag aaacagtata tccgccaaat ggcatccaat
     8281 ggctttgtcg gtattttcac ttccatgcat ataccagaag atgatgtttc ggcatatcaa
     8341 aagcgattga tcgaactagg aagcgaagcg aaaaagaata aactagaatt gatggtcgat
     8401 atttcaggag aggcattgga acgagcgggt ttttcattag aagatctaac gcctttgaaa
     8461 gcaattggtg taacaggttt aagaatggat tatcatattt ccaatcagca gattgccgac
     8521 tggtcacatc aattgaaaat cagtttgaat gctagcacga tcacgcctaa agatatcgat
     8581 gaattgaaag aagcagaagc tgatttctca cagattgaag cttggcataa ttattatcca
     8641 cgtcccgaaa cagggctgga caaagaatgg tatcagaaaa aaaatcaatg gctcaaaatt
     8701 caaggtctcc ttgtccaagg ttttgtacca ggagatacag agttgagagg cccactatat
     8761 caaggattgc caactttgga agaacatcga gggatccatc ctcttgcagg cgctctagat
     8821 ctattagcaa gtaacacgga tattgtttac atcggagatg caggtctgtc agaaaacgtt
     8881 caggagcaat ttgcttcttt ccaaaaagaa cagacagtct tgctccatac ggaacctgta
     8941 gatgaagaat tttacgaata tatattaggg aagcatacga atcggcaaga cgatgcaaga
     9001 gatgtcatac gaagcgtaga tgcacgtttt cgagagattc cgcctatccc agcaagaaat
     9061 acagctacgc gtatgaaagg gtctattact ttagataacg aaaagtattt gcgctatatg
     9121 ggtgagatcc aattaaccaa atatgatttg ccagcggatg aaaaagtgaa tgtagtagct
     9181 aaagttatca aagaagagct accattgatc aaccaaatca aagctggtat gaactatcaa
     9241 tttataagaa aagaaggaag ataaaaatga gcaaaatcaa tttagagaac ctaacaacgg
     9301 aacgcagaaa ccaagaaact tttggattag atgagatgag tatttctcaa gccgttcaac
     9361 taatgaacaa agaagatcag aaagtggccg ctgcggttga aaaagaactg acaagtatcg
     9421 aaccagtgat tgctaacacg attgagtcct ttaaaaaagg tggacgtctg atttatatgg
     9481 gagccggaac aagtggaaga ctaggtgtgt tagatgctgc tgaatgcgta ccgacattcg
     9541 gtgtagaacc ggaaatggtc ataggtctaa ttgctggtgg agaagaagca atgactgtag
     9601 ctgtcgaagg ggccgaagat tcttatgagc tagggcaaga agatctaatc aagctgaatt
     9661 tgacaaaaaa tgatatagtt attggtatcg cagctagcgg tcgtacacca tatgtgatcg
     9721 gtggattaga ttacgcaaaa tcgatcggtg ctgtcacagg aacgatttct tgcaataaac
     9781 aagccgaaat cagtaaacac gcggatttcc caatcgaagt cgatgcaggt ccagaatttt
     9841 tgacaggctc tactcgattg aagtcaggaa cagcacaaaa acttatcttg aatatgattt
     9901 ctactatcag tatgattggt ataggtaaag tattcaataa cttgatggtc gatgtcaaac
     9961 caacaaatga aaaactaatc gaacgctcaa aacgaatcat catgcaagcg acagaagctg
    10021 attacgaaac agctgaaaag tactttgaag aagcagatca aaatgttaag ctggctatcg
    10081 tcatgatttt gacaaatagt gataaagagg aagctcaagc aaaattagct gaagcaaacg
    10141 gatttgtaaa agggacctta tgagggggaa aagaccatgg caaaaaacga actgactttg
    10201 gaagaactgg caaaacaaat ttataaaggg gcaggcggca tgggcaatgt agaaagcatt
    10261 gtccactgca tgactcgtgt gcgtatgaag atcatcaatg atgatcttgt aaatgtctct
    10321 gaattaaaag cgattccagg agtgcttggt gtagtagaag atgaacagct tcaagtcatc
    10381 atcggacctg ggaaagtgaa caaagtagca cagcaaatgg ttgatttggc aggcgtgaaa
    10441 ttaggagaaa aactttcttc tgtaagtaca gctacagcaa attcatcagc atctggaaaa
    10501 gaccgtgtaa acgaacgggc acaagaaatg aaagcggcac aaaaatcgaa acaaaaacca
    10561 tctaaaatca aatcggtatt aaaagacatt tctaatatct ttgtgccaat gattcctgca
    10621 tttgtcggta ctgggatcgt ggcagggatt gccgcagttt tagcaaactt agtcacagcc
    10681 ggaacacttg atgcagcaac atggcaacaa tatatcgatg tgatgaacat cttgaaaaac
    10741 gggatgttta gctacctagt tatctataca ggtatcaatg ctgcacaagt ttttggtgca
    10801 acaccaactc ttggtggagt tatcggggcc gttgtgatgc tgacagggat gaatccggaa
    10861 gcaccactga aaaacttatt tactggtgaa gctcttgcag ctggacaagg tgggatccta
    10921 ggtgtcatct tcgctgtttg gctattgtca atcgttgaga aaaaactgca taaagtagta
    10981 cctgatgcgg tagatatcat cgttacacca actctttcat tgattgccat cggtctgatt
    11041 gaaattttct tcatcatgcc tttagcaggt ttcatttctg atggcatggt cggtgggatc
    11101 aactgggtac ttggcgttgg tggagcattt gccggcttcg ttctaggcac attattcttg
    11161 ccgatggtta tgttcggact gcatcaaatt ttgactccaa tccatgtgca aatgattgat
    11221 gaaactggtc gtacgctatt gcttccaatt cttgcgatgg ctggtgcagg acaagtgggt
    11281 gcagcattgg cattatggat tcgctgcaaa aaagataaag aattaacaga aatgatcaaa
    11341 ggagctttac cagtcgggat cctaggaatc ggcgaaccat tgatttacgg agtaacatta
    11401 cctctaggac gcccatttat tactgcttgt atcggtggag gtatcggcgg ggcagtgatc
    11461 ggaatgatcg gaaatgtcgg ggctatcgct attggacctt caggagccgc tttgatccca
    11521 ttgatctctg acggtaaatg gttaggctac atccttggat tattggcagc ttatgctggt
    11581 ggcttcgttg ctacattctt cttcggtatt cctaaagacc gtttagaaaa agaagaatta
    11641 gcgactgaaa cagccacagt aaatgaagtg cctgaaaaag tagtacctca aacttcagct
    11701 gaaagtgtga ctatcctctc atcagttgct gacggaacag ttgtttcttt agataaagca
    11761 agtgatccag tttttgcaca aaagatgatg ggtgaaggct actttgtaga accagaaaac
    11821 ggacatatct attctccagt tactggggta gtcagctctg tcttcccaac gaaacacgct
    11881 attgggatca caacgccaaa tggtttagaa atcttgcttc atatgggaat caacacagtt
    11941 gacttaggcg gtacgccatt tgatgtgaaa gtaacagaag ggcagcaagt gacacctgat
    12001 acgcttgtag cagaagctga cttagaagcg atcaaagcag caggcaaaga aacatccatg
    12061 atcgtcttgg taaccaatat ggatcgtgta aaaaactttg tcttggaaaa aacaggtaaa
    12121 gcaaaagcaa aagcacctgt gatggatgtc aaagcttaat atgatagaaa caatagaaac
    12181 gaaagaagaa actgaaatct atttttcagc ttcttctttt ttattattta ttgttttctg
    12241 ctaaaatctg ataaattcgt gtcacatcag atggtgttcc ccgtaattca taatctgcca
    12301 agaaaggaaa gccaaatgtt tgtgcatatt gtttggctgt tagacaatac tgattattga
    12361 agtttttatt tccgctaccg actacaccaa gacaaagttt atgattgtct tcatgttcca
    12421 gatattcacg catcgtttcg gtcaagattt ctgtatcacc gttatctaaa ccatttccgc
    12481 cgtctaaata agtaggaacg aaagtaaaaa atggttctgt ttctttcgta aaatcactat
    12541 tttcatggat ttcttttaaa gtaacttcag gattgacagg atcttctgca tgcatttttt
    12601 cagcatattc tgctagatgt ttggcaaagg cacgcgtatt tcctgaaatc gatatatata
    12661 aaatgttcat ggatcatcct ccaattcttt ccttttagta tagcatcttt tttaagtttt
    12721 ttggtggtgt aatttggaaa gggactaggc gctttgcttg tataatgaga aaaaagccgg
    12781 tagaattata tttctattaa agtttttatt aattaagtaa aaataaaaaa ggtttgttat
    12841 actttttgat aaaagcggtt ttattttgga ggatgcttac atggatacag agcaacagaa
    12901 aaaaggaatt ggtttaattc cacttgctgc cttagtaatt ggttcagcga ttggaggtgg
    12961 cgtatttggt attatgacgg acatctccgg atcggctggc ggcccagcta ttttcagttg
    13021 ggtactcgta ggaacagcaa tgttgatgct ctccttgtcg atcaataata tcaataaaaa
    13081 acgtccagat ttaaaaggcg ggatctttag ttacgcagaa gaaggttttg gccgtttcgc
    13141 tggttttatc agcggttggg gttactggct cacatgctgg ttaggcaacg tagcttttgc
    13201 aacattgttg atgagcgcat taggatattt cttcccagta tttgaaggtg ggcaaaatct
    13261 ggcatcgatc gtgatgagta cagtcttttt atggggatat gcatggctag tgaatcgggg
    13321 agtcgaaaat gctagtttcg tcaatgctat cgtcacattc tgtaaattag tgccattatt
    13381 tttatttatt gtagtagcta tcagcacgtt ttcgatccaa caattcattg ataacttttc
    13441 tagcgacatt atattgaatg cgaatggaca gccgatcccg ttttccagtc aagtattgag
    13501 ttgcatcatg gtgatgcttt gggtatttgt tggtattgaa ggagcctctg tcgtaggttc
    13561 acgagcaaaa aaacaatcag atgttgggaa agcaacgatt ctagggatat tcggcttgat
    13621 tattatttac gtcttggttt ccatgcttcc atatgggaca atgacgatga atcagctgca
    13681 aacgatcaag gcgcagcctg ctatgggcta tgtgtttgaa atgatggttg gtaaatgggg
    13741 agcagtcgtg atcaacggcg gtttgatcat ttctttagtt ggtgtttggc tctcatggac
    13801 gatcttaccg attgaaacaa tgggaaacat ggctgaagac ggacttttgc ctaaagcatg
    13861 gggaaaactc aacaaaaatg gggcaccaac ttatgctatc gtattgacga cgttatgtac
    13921 gaatgtcttt ttactttcat tattattcac tgacagtgct tataactttg cttacacact
    13981 tggtacagca gctattttct tcacgtggct tttcttagga ttgtaccaaa tgaaactttc
    14041 ttatcagcgg aaagaatggg tccaattttt agtagggctt ttagcaagtg cctttcaagt
    14101 atgggctatg ctagtcgtag cctttaatga agtaatgctt gttcttgttc ttttcttgcc
    14161 aggcatttat ttttacctca aagcacgtaa ggagcaaaac gagcctgtac aacatatcca
    14221 cttagataag attttactta taggtgtgac gatggtcgga gtgattgcaa tggttttatt
    14281 tgccactggg atgcttcacg tatagaagta taaaagaagt ataaagagag gttgtgagcc
    14341 tgtcgtttat tcaaaaacat actaggaaag gagctgtgac acaaatgttg tcacagctcc
    14401 tttttcagaa taaacggtgt ttaagagaca gccaatcggc aataaggctg aacgtctctt
    14461 tcacagcctc ttttttgatg agtcatcata cattttttcg cttaaatacg agatatccta
    14521 atgctaagaa caaaacgatg taagctaaat taccgaatag catttgatta gtagaaagat
    14581 gagtcaattg cttcatgatc tcttcgtttc caatttggtt ttgcagattc aacatactga
    14641 tcggattcca tttgatccac tcccattttt ggatgagtgc aataaggata ccagatatga
    14701 ttgacgaagc aaaataaaag acgattcctg ctgagatgga tgcgccggaa ctattgataa
    14761 agcaagcaat catcaacact aaacttaaaa tcagccataa accgatataa ctacccaaag
    14821 tataagtgaa caagctttgg atcagtgttt gaccagtaga aaccttctcc gtgaaggaaa
    14881 tcgaagggaa aagaatcagt ttcatcaaca cagtgacaat aatagctaaa aggtataaat
    14941 aaacagaata aataactaat gtgatccatt tgcttactaa tatttctcct cgagaatact
    15001 ttcgataaag gagatttttc aatgtaccat attgtgcttc catcgaaatg atcgtgcttg
    15061 cagctgcaat catgataaag acaatccaag atgtggcact aaaaagctga gcagtcattt
    15121 caactggatc aatgatcgtc gtgtattttt tggctagaag tgctgttcct atgagcaaaa
    15181 caactaatag aacagataaa acagcagttg actttttctt gatcaattta aataattctt
    15241 gttttacaag caatgtcatt tttcttcttc cccctttact gaatccagaa tttccaatag
    15301 agaagattcc aaatcattcg tcagatgctg gacgtctgtc acatggatat tttgtgcaga
    15361 caatagctgg atcacggttc ctaataattc ttcgttttct tttaaaagct tgattttatc
    15421 aggatcttcc aaagtatatc cagcttctgt caagatcgtt cgagcagcta gatcatttgt
    15481 tgtagagata acgatgaaat cttgcgctgt ttggttcaat tcagccattg tacattgttt
    15541 gatgatcttt ccttgattga tcaggatcag ctcatcgacc attttttcaa gctcgctgag
    15601 taagtgactg gagatcaaga aagaggtgcc ctgagtagcc aagtcggtaa tgatttttcg
    15661 cacatctctt gttgcttgtg gatccaaacc gttcatcggc tcatctaaaa taacgataga
    15721 cggatggttc agtaaggcaa gagcaattcc caatttttgt ttcattccca acgagtagga
    15781 tttcgctttt ttgtgaatgt acttttccat cttgagctga ttcacgatag tgtcgatagc
    15841 agatacatcg ttggtttcag agaataactt cagatgatca tagcctgtta agaatggata
    15901 gatacccggg tattcgatca atgctcctac ttgttccaac cctttatgac tagagggaga
    15961 aatctcattt tcaccaatac agattgttcc atctgagtaa tggatcagtc ctaaaatagc
    16021 tttcatgatc gttgtttttc cagcgccatt aggacctact aatccaacga ttttgccagg
    16081 ggataagcta aaactgacat cgctgagggc ttgtttgtga ccaaagttct tggataattc
    16141 ttggaccgaa agacttttca aaaatattca ctccttttct atagatagta caagcataca
    16201 cctgatgagt ggagaagtcg ctaggtcatt ggacttattt cttatttttt cataaaaaaa
    16261 ttgaatatta aataaaaagg cttatttttt ctgatcagta cttattccaa gtgtctagtg
    16321 gccgctagta atcttgatgc caatgatact gatgagtagc atccccacaa agaaccaagt
    16381 ggaaagattc agcttatcat gaaacaagac cacaccaatt atgaccgaac caactgcgcc
    16441 aatccctgtc cagataggat aagagagact catcggcaaa cttttgattg cctttgacaa
    16501 aaagtaaaaa ctagcaatca tgccaatcac tgtgtaaaaa gaataattga ttttagaaaa
    16561 accatcactc catttcatca tcgttgacca gaatacttcc aaaattcctg ctatgattaa
    16621 ttccatccat gccatactta cattcctcct taactataaa aaaatacaca aaaagaggaa
    16681 aatcatccct tcaaagacct aatggaatga tcatcctctt tatcctaccc ggtggcagat
    16741 tgtatatctt tttctgtagt atagcacacc tatccagata tcggcaatct catacttttt
    16801 agtaaagtgt accaagcact gacggatata tcgttagcgg aaaagaagat gaatgctttg
    16861 caatttcata gaaagaagtt gttatttatt aatcatttaa aaaaaacagt aactagacct
    16921 tatttctata tattatcggg caataaatta aaaaaagctt taccttacaa taagtaagat
    16981 aatttttgtg ataaaaaaat aacgaaaaat atttaaaaat agtgtctatt tcgtgagaaa
    17041 acaactatat tttttatgtg ttttgcttta taattaaaaa aatattgtca ttttatggag
    17101 gaaaatatat ggaaaattat cttagccttt taggcgtctt gattgttatt ctcgggtttg
    17161 catttaaatt agactcgata ttgatcgtaa tggttgcttt agttgttaca gctttgactg
    17221 gcggtcttgg cattcaagga atgttagaac ttttaggtac aagttttgta aacaatcgcg
    17281 ggatggcgat ttttatcatc attatgttgg caacaggaac gcttgaaaga aatggtctga
    17341 aagaatcagc ggcttccttg atcaagcgat tcaagaaagt atctgctggt atgattgtag
    17401 atatttatgg gatctttcga atgatctttg cggcttttaa cgtgagtttt ggtggagttg
    17461 ctggatttgt tcgtccaatc attttaccga tggcattggg gaccatcgaa tcaaaagggt
    17521 tagaagtcca tcctaagcat gaagaagaac taaaaggtat ggcttcagct atggaaaatg
    17581 tctgctggtt ctttggccaa gtactattca tcggaggagc aggggcgttg ctagtccaat
    17641 caacactgaa agatctagga tatgacgtaa cattgggaca gcttgcttta gtggaagttc
    17701 cagtggctat cacggcttta gttgtggcaa gtgtctactt ctatcttaaa gaaaagaaat
    17761 tagcgaagaa atattatcca ggaaaggggc aataagatgg acttttttac aaatcctgat
    17821 attttattag gcgataaatt actggagatc ctttatatcg tgatgggact cgtattgatt
    17881 tacactggag taagaaattt aacagataaa accaatcctc atcgttatgg gtcagccttt
    17941 ttctggacgg tcttaggggt agttattgca ggaggaagat ggcttccggc aatcgtgaac
    18001 ggtggcttga tctttgcgat gaccatacca gcaattgcta agaaagtcag caaaggagaa
    18061 agccggcttc catcaaaaga atatatggaa aaaatgtcag acaaactagg aatgaaaatt
    18121 tttattccag ctttgagtat cggtgtcttt gcgatcttgt ttgctctatt taccaattta
    18181 ggtgctttag tcggcgtggg cgtcggtgtt tttgcagcca tgctaatctt gatgttcttt
    18241 tctcgagata atcgaccaag tacattttta gatgatgcag cagatatgct gggcacagta
    18301 gggccgttaa gcatgttgcc aatgctttta gcttctctag gtgcggtatt tacaagcgct
    18361 ggagttggga cagtgatttc caatgcagta ggtacgatcg ttccagaagg aaatgtgaac
    18421 atcggcatca tcatttacgc tcttggcatg gtgatattta cggtaatcat ggggaatgca
    18481 tttgcggcca tcaccgtgat gacggttggg atcggtgcac cattcgtatt tagtcatgga
    18541 gcgaatccag cgttaatagg aatggttgca ttgacttgcg gtttttgtgg gaccctgctg
    18601 acaccgatgg ctgctaactt caatatcgtg cctgtagcga tgctggagat gaaagacaaa
    18661 tacggagtga tcaaaaacca gttgtttatt gcgttattta tgctagtttt ccaaattgct
    18721 tatatgattc tatttaaata atagaagaga ggggttgtga cagaataatt gtcgcaacct
    18781 cttctaacat tgtagtggaa cactattttt ttataagaat atattaagtt tgatctgtta
    18841 tgttatttaa actattgttt cctttattta cgctgttcaa ggaaaatggt atgataaaag
    18901 ataaccatat gttaaatcaa tcggacggag gaacaacaga tgaaagtact agtaacaggt
    18961 tttgatccat ttggtgggga caaagtgaat ccagcctatg aagcagtaaa aaaaatgcca
    19021 gatgaaatca gcggtgctga aatcatcaaa gtggaagtac cgaccgtatt cgaaaaaagc
    19081 agtcaagtag tcaaagaagc catccaacag caccaaccag atgtcgtgat ctgtgtcgga
    19141 caagcaggtg ggcggtcagc agtctcgttc gaacgtgtcg caatcaatct agcagaagca
    19201 agaatccctg ataatgaagg aaatcagcca ttcgatacgg ctttagaaga aaacggtcca
    19261 gccgcttact ttacctcatt gccaatcaaa gcaatgacaa aaaatgtaca agatcatggt
    19321 ctaccagcgt acatctctta tacagcagga acatttgtct gcaatgatat catgtatcgt
    19381 ttgcttcatt tgatcgaaac acaattcccg gcaattcgag gtgggttcat ccacgtgcca
    19441 ttctcacccg atcaagtgat cgatcgtcca gtgggtacgc cttctatgtc tttagaagac
    19501 attgcttctt ctttgactta tgcagttgaa gcagcagtag aaaataaaga agatatttcc
    19561 ggaaatgccg gaacaactca ctaatttagc aagaaacact ggctggttgc taacgttatg
    19621 ttagtagcca gctttttgta attagaaagg aaagaaacag ccgtggaaga atggcaatcc
    19681 gtctttgaag aatggtttcc aaaagaaatc agcaaaagct atcccatcaa aatttctaaa
    19741 caatacacaa gcagtcaacg ttgggagatc tatgcaaaat taacgaaaaa acagcgggaa
    19801 ctagtcgata agcaccgccg atatctaatc agctctcgtt ttatggaaga acattattta
    19861 gctgcaacag attgggtgtt ttctgacttc aagatcaatc cgttttttcg aacaaaacga
    19921 agccagcaaa aattgtattg tgagtgtggt agagaattaa aagtgcaata catcgttaaa
    19981 tcgccaaaaa ccggaaagat cctaaaacta ggcatcaacc actttgccga tcacctgcat
    20041 gtctctccga cagtggcagc ttcgatccat cagggaatga caaaagttga tctggcctta
    20101 gacgaattat tatggcttaa gcagaagaat atcgattttc ctgaaggact atggcaaaaa
    20161 tactgttttg tcttgtacca aaatcgtcgg atgaaacagc catatttacc ggatataaaa
    20221 ttagctcagc ggctcgcaga atttcgccaa gtagaaatgc cgatttacat agcggattat
    20281 caagcattgg aaaatgaaat caagaaaata agtgaacaca tcaatgggca gtcaaaaaag
    20341 cgacagataa aaaaagaact atttgacgac tttgcagaag aattagtaaa ggatgtagaa
    20401 gaattcttga tcaactatcg cgcattttta cgtaaagatt ggcaatccat cgtatatgaa
    20461 gaagtgccag tacatcctaa tgcttatttt gaaacattta tttccgtgtt gagaaagaca
    20521 aaacgtcaac gaacaccaga agtcactgct cagatggaat attttgcgaa aaaccaacga
    20581 ttcatccaac caaaaatcta tctatttatt tggaagcaat attgtcgcta tggttttaca
    20641 gaagggttct tcgacagcat tcctcggata gtcagaaatg gttttttaaa agtattgcgt
    20701 aaagaacgag aagctataca atctgctgat aagaaagacc gtacagtctc aaaagaaaaa
    20761 tggcaactag tagtgaaaga tatccaatct ggaaatgtgc aagagacaat cgacaaatgg
    20821 aaaggaaaac attatcgttt tactgaagct cagaaacaag cgttggaata ttatcagaag
    20881 ttagaagaat cactgcgttt caatgatgag gcaagaaagt atctgaagga attactataa
    20941 aaaatagagg ctgggacaga agcgtttaac tccaagaaat aagaa
//
LOCUS       DACGLW010000204        20862 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.guided.048, whole genome shotgun sequence.
ACCESSION   DACGLW010000204 DACGLW010000000
VERSION     DACGLW010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20862
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.guided.048"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            267..1304
                     /locus_tag="IXT53_002980"
     CDS             267..1304
                     /locus_tag="IXT53_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ4906720.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(1393..1716)
                     /locus_tag="IXT53_002981"
     CDS             complement(1393..1716)
                     /locus_tag="IXT53_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HAQ4906721.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(1703..2410)
                     /locus_tag="IXT53_002982"
     CDS             complement(1703..2410)
                     /locus_tag="IXT53_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HAQ4906722.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            2496..2837
                     /locus_tag="IXT53_002983"
     CDS             2496..2837
                     /locus_tag="IXT53_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HAQ4906723.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SMEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAAEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            complement(3008..3856)
                     /locus_tag="IXT53_002984"
     CDS             complement(3008..3856)
                     /locus_tag="IXT53_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906724.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            4454..5317
                     /locus_tag="IXT53_002985"
     CDS             4454..5317
                     /locus_tag="IXT53_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ4906725.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            5417..5899
                     /locus_tag="IXT53_002986"
     CDS             5417..5899
                     /locus_tag="IXT53_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HAQ4906726.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(6162..6629)
                     /locus_tag="IXT53_002987"
     CDS             complement(6162..6629)
                     /locus_tag="IXT53_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HAQ4906727.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            6752..7603
                     /locus_tag="IXT53_002988"
     CDS             6752..7603
                     /locus_tag="IXT53_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ4906728.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            complement(7854..9812)
                     /locus_tag="IXT53_002989"
     CDS             complement(7854..9812)
                     /locus_tag="IXT53_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HAQ4906729.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(9809..10681)
                     /locus_tag="IXT53_002990"
     CDS             complement(9809..10681)
                     /locus_tag="IXT53_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HAQ4906730.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            complement(11023..11895)
                     /locus_tag="IXT53_002991"
     CDS             complement(11023..11895)
                     /locus_tag="IXT53_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HAQ4906731.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            12023..12592
                     /locus_tag="IXT53_002992"
     CDS             12023..12592
                     /locus_tag="IXT53_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HAQ4906732.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            12659..13396
                     /locus_tag="IXT53_002993"
     CDS             12659..13396
                     /locus_tag="IXT53_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HAQ4906733.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            13627..14895
                     /locus_tag="IXT53_002994"
     CDS             13627..14895
                     /locus_tag="IXT53_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosaminidase"
                     /protein_id="HAQ4906734.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            15382..15846
                     /locus_tag="IXT53_002995"
     CDS             15382..15846
                     /locus_tag="IXT53_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HAQ4906735.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            15830..16459
                     /locus_tag="IXT53_002996"
     CDS             15830..16459
                     /locus_tag="IXT53_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HAQ4906736.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            16813..18291
                     /gene="msr"
                     /locus_tag="IXT53_002997"
     CDS             16813..18291
                     /gene="msr"
                     /locus_tag="IXT53_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Msr family ABC-F type ribosomal protection
                     protein"
                     /protein_id="HAQ4906737.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            18338..18784
                     /locus_tag="IXT53_002998"
     CDS             18338..18784
                     /locus_tag="IXT53_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HAQ4906738.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(18983..19498)
                     /locus_tag="IXT53_002999"
     CDS             complement(18983..19498)
                     /locus_tag="IXT53_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HAQ4906739.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            19615..20816
                     /locus_tag="IXT53_003000"
                     /pseudo
     CDS             19615..20816
                     /locus_tag="IXT53_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT         6631 a         3703 c         4315 g         6213 t
ORIGIN      
        1 caaggagcaa tttcaaaaaa cagcttctca tgtttgatga atattttgac ttgtgaccga
       61 agagttggta ggagaacacc gtttataaga ggttgtgaca aaagtcttgt cgcaacctct
      121 tttttaacca aaaaagtgca aatagtttac tttaagtaag agaataatta tgatgaataa
      181 aaaaggtgga acaaatagca tactatatat attgaaatga acactgtatg gatgctccaa
      241 cttgatgaag ctaaggagga attcgtatgt cagaaaagga attaatgaag gtagtcggtt
      301 tttatgaagg gttgccgatc gatgatccaa aaagctttat tgatgaaaaa gaacagatac
      361 ctgtacccag ttcacgggat cttctggtaa aagtcaacgc agtatctgtt aatcctgtcg
      421 atacgaaact tcgccaagat aacgggataa gaaatgcttt acgtatctta ggttttgatg
      481 gagtcgggaa agttgttgct gtaggtgacg aagtgcaaaa attttctgtc ggcgatcgcg
      541 ttttttacgc aggtacaaca acaagagcag gaagcaatca agaataccaa ctagttgacg
      601 aacgtattgt cgctcttgca ccaaaaaatc tttcagatga agaagctgca gccttgccgt
      661 tgacctcatt gaccgcttat gaattgttat ttgaaaaatt tggtctaact ccagaagaaa
      721 atgccaatcg cgggaaaaaa atcttagtga tcaacggatc cgggggcgtc ggttcgattt
      781 tgaatcaact agcgcattgg gctggcctag aagtctacgc gactgcaagt ccgaagaatt
      841 ttgaatggct gaaaaaaaca ggtgtagact atccaatcga ttatcatcac gacttaagta
      901 aaagttttcg tgaattagga atcgatcaag tagagtatgt cgctgtatta ttcgatatta
      961 cccgctactt tgatcaaatc aagcaccttg tccgaccatt tggacatgtg ggaacgatcg
     1021 tagggatcaa agagccgtta gatataggtg tttggaaaaa ccaatctatc agcttcgact
     1081 gggagtatat gttcgctaaa acagactata actatgaaat cgaaacgcaa ggagaagctt
     1141 tagcgcacat tgctgcttta gcaaatgaag ggattatccg cagtacattg agcaaggtct
     1201 attcagatgg gatgaatgcg aagaacttga aacaagcaac caaagacgta gaaacaggac
     1261 atatgttagg aaaagtcgtt gttagtggtc cgttcaacgg ataagcaaga atacgccgat
     1321 ttttttctta tttgactttg ataaaaacaa gagcgcatga cagtaatttg ccataagctc
     1381 ttgttttttt atttataata agcgaatcaa cgaaagggaa agaattcctg ccaagacgat
     1441 caccaataag ctttttgaaa gaatagcagc taaaagtgta ggaactgaag ccaaagcatt
     1501 ttcgagattg atctgcggca gatgtccgat attttgggta aatagactgc tgaaccataa
     1561 agcagacata atgacgatcg ggacaaaact taaatactca agcaacggtt gcggtaaatc
     1621 gaattttttc agtaagacaa atggtaggac cctagaaagc caagttgcta aagtacaacc
     1681 taaaatagtc agcaatgtaa actcaaaaga aggcatgttt gatccacacc cccaatccgc
     1741 acccaatcag tgttacaact acaatcacta agttgcttgg gataaaaatc agtccgacat
     1801 ataccagaat caaagtgaga ccaatcagga tcaactgtaa tcgtttagag gtgtttctat
     1861 cagaaatgac ttgaagataa agtaacccga taaacatagc gacgatggcg aaatccaaac
     1921 caaacttttt cggatcagta ataaaattcc ctaaaagtgc gccaacaaga gaagaaccca
     1981 cccacgtcaa ataagaaatc caattagctg tattcatcca acgaaagttt aacttttgtc
     2041 cagtatagtt caatttattc attcccagtg cgaaactttc atctgtcagc aatgtgccaa
     2101 tcagcagatt ttgaagcagc cgatttttct tgaaataagg agcaatcgtc atactcatca
     2161 gaatcattcg ggaattgact agaaaggtcg agaaaacgat ggaaagcaat gggctgtgac
     2221 tagcaagcat gctgactgtg ataaattggg cagaaccagc atagaccagc agggacatca
     2281 acgtgacgac tagtggatga aatccggcag ctttcccaac gataccaaat gcaagtccaa
     2341 taccgatata accgaaaacg gtaggtaacg tgtctttgat cgcagttttt atatctaact
     2401 tttcatccaa taacaaaact cctttagcac gacataaatt ttcataattg tcgttaaact
     2461 tgtgaatatt ctaaattttt aatgagcatt tgtcaatggt gggattgaaa cgtaaagaac
     2521 tagcgggtcg cagatttaca cgtattgttt cggtgggagg aggcgcgaaa aatcttgcat
     2581 ggctacaaat ccaagctgat attttcgatg caacggtcta ttgtttaagt atggaggaag
     2641 gacctgcatt aggcgcaaca atgattgctg cactagggtt agggtggttc gacacacctg
     2701 aaagttgttg tcagaatttt atccaatata cagatgcagc ggaacctctc ttacaaaatg
     2761 tagaaaagta tcataaaatc tatcaacaat gccggaaaat ctatccagca acgaaggaaa
     2821 tttgtcatga cttataaaac aaaacgcgta cctaatagtc aaatgactaa tattgggtac
     2881 gcgttaagtc ttctttactt catcctttgc atgattcgtg tttttaaagc aaggtctgct
     2941 gtatttcttg tggatttagg atggttgtta tcgtttactt gctatttttc attgaaatcg
     3001 tctttcttta ccgtttcttt agttttttat ctattttttt cttttggtta gaaaattctt
     3061 tttcccgaaa gtcttctttc aggattttta tagagtagcg ttttgaataa gaggcgtcag
     3121 tatgatcgac ttggcagacg aaccaagaga acatttgcct tttttctgca tgaggtgctt
     3181 ctgctaataa ttctttccat aaataataac agtcctctat cagaagaagg gttcgttcat
     3241 cctcaggctc ccaatagaat gtttcgagag tagcaatcac ttcattgatc aattcgaatg
     3301 cgggaagata ggcattttgc tgaataagcg attcaactgc ttcttggatg aactttcgca
     3361 tctccatgat gaacttcgct gttttcctgt ttttgttttt cttgataata tgatcgtatt
     3421 tatggatgat agttgttaat ttatctgaat aatttattgc atgaaatggc atcttaactt
     3481 tgaagcgttc gataaagtgc ggatgttcta taagaatctc tgttaaaagc tcccgtacaa
     3541 gagaggaact agcggaggaa acaagggacg agagttcgga aagtgagtag ttggattttt
     3601 ttattggacg ttcttcgtaa gcgaataaga cagcagccat gtgtttgcat ttttttcctt
     3661 ctatggcata tagacaatca caagtcatgt tggtcttttc atcccaagtg atcactacct
     3721 tataatcttc cgtgccagaa actgttgcgg acaagtactt gcttgtatag ttcaaatgac
     3781 ggacaaaacc gtgcagatga tagctgtaac cgcgttccaa aacgttttca ggaaaatact
     3841 caagccaatt attcatctag ttctcctttc atgaaacact tatttatatt tagtataaag
     3901 aaacgtttaa gaaaaaagta gctgatttta catatttgat aatcactttt gctcgaattg
     3961 tcaaaaattc attctccttt tcttttctta gagaatgtga gaaaaagtaa aagaatagcg
     4021 tgttttaaag atggaacttt ttttgttttt tatttatctg acaaaaatga agtgattttg
     4081 ttagaaataa ataattgttc caattgataa aagaggtatg atgtatcgca aagaaaaatc
     4141 caagcagcgt tctgatcagc agtttgaaca acaatttgct ttgtacagta ggtagtaagc
     4201 ggattaacta agggaataaa agatattttt accgctttgt ttacttagaa tctgtcttcc
     4261 ccaaaattta tttttctaaa ggtaaaacat agataggcag ataataccaa ttatccgtta
     4321 cgaaacgctg cttgagaatt ttctttagat ccatacaaca ttttctacat ctgactaaat
     4381 tggttcctga ttcatcaagt aattgacttt ctttgataat atgataaaaa ggcaatcaag
     4441 gaggaagata aatatgctta aagatctgaa ccaattgatc gattacatcg aagaacattt
     4501 gacagaagga ctttcctata aaataatagc cgaaaataca ggaatctctg aatatcaatt
     4561 aaaacgcatc tttacgttta ttgcagggat gtcgctaatg gactatatca aaaaccgtag
     4621 attggctttg gctaatcgcg atcttctaga tggatcaaga gtgacagaaa cggcgtttag
     4681 atatggatat gaaacagtcg aaggtttttc aagagctttt cgtgattggt caggatatct
     4741 gccttcagaa atcagtagat acggcataca aaaatctttt ccacgcttga cattttttat
     4801 tgatgtcaga ggaggaaaat cgatggaatt tcgtatcgaa aagaaagaac agttttatgt
     4861 ggcaggtgtg gcgaaaaaag ttcctgtcaa atttgaagga gtcaatcaag aaatacaaaa
     4921 gttagcgcag acgatcacgg agactcaaag ggaagagatg cataagttag gagatttata
     4981 tccaaatcaa gtaatcaacg tttcttatgg atttgatgga gaccgcttgg aagaaaaggg
     5041 cagtttgatg cacatgattg gctttgctac gacaaaagaa aatccgtacg ctgatttaga
     5101 aatgcttgct atagaagaac acacttgggc catttttcct aataaagggc catttcccca
     5161 aactttacaa gatacttggg ggaaaatcta tgcagaatgg cttccttctt cagattatgg
     5221 gttagtcgaa gctcccgaga tctcttttac aaaatgggga gaggatttca gtaatgttta
     5281 cagtgagatt tggattgcag tgaaagcaaa aaaatagtta aaaaataaaa atatccctag
     5341 aaaaaagagc gtaatcgtgt catttccctt taattttatg taagatgaac ttaatataat
     5401 agaaagggtg ggagaaatgg caaagtatag gatcgaagaa aaaccagatt ttacggtgac
     5461 gggtattggt gtggaattga agagtagcaa tacggatttt gtcggtttgg ctgcagagaa
     5521 acaagcattt gagcaaaaag ttatcggtaa tggaacaata gatgcattga aacaagctgc
     5581 tgaaaatcat tatttcttta cagtcaacga accctataac gggaaaatga tgtactatgt
     5641 cggtgttcaa acgagtcagg atgttccaaa cactacgcga atcatcccat tcccaaaagg
     5701 aacgtatctt gttgtaagcg ctttagcaga atcgcctgaa gaattaagaa atgatttgac
     5761 gaatacggcc tttgggcatg ttttaagaga agtagaagat tacgcctatg ttgggggtcc
     5821 aaatgctgta gtcgaaatgg gagttaccaa cgggaaatat atgggagaaa tctggatacc
     5881 ggtagtcaat aaaccatgat aatatagagg tcgtgaaagt agcgctttga cctgagcatt
     5941 agagaggaaa aatgggaaat agctttccat attttgcatt tttcagaact aatgccgagg
     6001 gtcagctact tgaacaccgt ttatcaagac gtcatgactt cagcgctttg acctgagcat
     6061 tagagaggaa aaaggggcat gacaaggctt ttgtcccccg gagaataaaa gaatttatgt
     6121 agacgattcc gatcagaaaa atgcaggcac agtagagaga attataaaaa tctctctacg
     6181 aggcctgtgt tttctggtct tttttctttt tccaaaacgg ctattttatg tatcagacta
     6241 tcaagtgtga tcttagccat ttcttcttct gcctttttct gcagccgagc gtagctttct
     6301 tctaaagctt cttggatatt cgcaccaacg acgcaatttg gatttgtttt tggatcgact
     6361 tggataagct gtgtattacc ttcgatgctg cgatagacat ctagtaaaga aatctgatct
     6421 ggacttttgg caagggaagg ctgcggtttt ccggtttttg taaagatcaa accggatttt
     6481 ttgagctgac tcatgatttt acgtacattt gctgcatttg tttcgatact actggcaatc
     6541 atctcactag aaagtgatcg ggtatcttga aacatttcaa tataggcaag tacatggatt
     6601 gcatcactaa attgcagaga atacttcatc agttgtcatc ctttcatttt ttttgcttga
     6661 cttatcttac cacactcttt atgataaagg tgtaatttat aaatgtacag ctgtaaatct
     6721 taacaaataa aaagaatagg gagtggcaaa tatgcattat ttagtaacag gagcaactgg
     6781 aggttttggt ggttatgcgc tggaattctt aaaaaagatg gttccacaaa atgaaatcta
     6841 tgtactagta cgtagtgaag aaaaaggaga agcactgaaa gaagctggat ttaatatacg
     6901 tattggtgat tatgatgatt tagactcaat gaaagaagcg ttgaaaggaa tagatcgatt
     6961 gctttttgtc tctggcgttc caggaaatcg tcaagccgaa cataagaatg tagtgaaggc
     7021 agcacaagca gctggggtat cttatatcgc ttatacaagt tttgcagatg ccgatcactc
     7081 gatcagtgta ttagcgccag atcaccaatt cactgaaaaa atgattaagg attcaggtat
     7141 ttcccataca tttttacgga ataattggta tgtagagaat gaattaccta tcattgggca
     7201 ggctttaaaa actggtcaat tcgtttatgc tgcaggaaat ggaaaaacag gttgggcatt
     7261 gaaacgtgaa tatgcagaag ctgcagcaaa agtactagta gaaagtgact ctccagaaat
     7321 cttagaatta tccggcaatc taactgacta tgaagaatta gccaaggcat tagagcgtgc
     7381 gacaggtaaa gagttagaaa ttattgaagc aagtgatgca gcgtttgtcg aaaatctaaa
     7441 agaagccgga ttccctcaag aagcagctga catgttttta gcgatccaac atgatattaa
     7501 aaaggatcag ttagctattt cctctgatga tttagaaaaa gtattgggaa aaccattaac
     7561 ttctcttgaa gcagcattaa aagaaatatt ggatcaggaa taatgtataa agaggttgtg
     7621 aaaaagccga ttagttttga gtatgaagaa aaattttgtg aaaaagctgt tctgcatcaa
     7681 gtaataagaa gagtcaaata aaaaatagct cttcatatgt ttcgtttagc tggacttatt
     7741 accgcggagt tggcaggaga acgccgtttt tattcggaaa aaggagctga gacaagattt
     7801 ttgtctcagc tcctgtttgt ttatcgataa aaaagtctgg ggaagacgat ggctcaggct
     7861 ttgttggaaa cagagccgat tttatcattt gcttttacat cacgtgcggg ttcgatttct
     7921 aagctttgga ctttgtcgcc atttgtgaat acaacgacca tatccgtgtt tttgcctgca
     7981 gattgaatag cggcaagatc aactaaagca atcaattgac ctcttgcgac tttttgacct
     8041 tcttctacat ataaagtgaa tggttcgcct tttaagtcaa cagtgttgat gcccatatga
     8101 agcaagactt cgataccagc atctgtttgg attccgacag catgtttcgt tgggaaaatg
     8161 tttgtgatcg taccagcgat tggagagaag atttctccat tttctggtaa tacagcatag
     8221 ccgtcaccca tcattttttc cgcgaataca tcatctgaaa cttcagtgat tggaatcagt
     8281 ttcccatttg cgatagcgta aatatcttgt tcgatcgttt caccggcaaa tgtttctgtt
     8341 ttaaccacgg tgtttgtttg tgcaggtcgt gctgcagcac caccatgtag ttttgccatc
     8401 tcatctgcaa cgaattgtac ttctgtacct acgatgactt gcgcattttt tccgtcgatg
     8461 atcttcatac caggtacgcc agtggctttg attttatttt gatcgatcag gcctgtatct
     8521 ttgacttgca aacgaaggcg tgtagtacag ttatcgatcg aagttacatt gtcggctccg
     8581 cctaatgcag cgtagatttt gcttgcttgt gccgcatatt tattgtctga attagtcgtt
     8641 gcagtatcgt catctgaaag atctgcttcc tcgcggccag gagtcatcag atggaatttt
     8701 ttgatagcaa agttaaagcc aaagtagtaa atcactgctg ttactaatcc ttggacgatc
     8761 aacatcaatg gctgattcgc aatcggattt ttcaaagata ggaagtaatc gacaaatccg
     8821 gcactaaagg caaatcctgc tgtccaatgg aagaatgcac tcactgctaa agaaatccca
     8881 gtgaaaattg catgcaagac atataatggc caagcaacga acataaatga aaattcaagt
     8941 ggttctgtca caccagtaaa gaatgaagca aaagctgctg ctagcatcaa ggaagctgtt
     9001 tttgctttgt tttctggacg tgcatttctg taaatagcat atgctcctgc tggaagcccg
     9061 aacatcatga ttgggaaaaa tccagcttga tacattcctg ttacgccttt tgttccttca
     9121 gaagccaaaa atttaccaat atcattgatt cctgctacgt caaaccagaa cacggagttc
     9181 attgcttgat gcaatcctgt agggatcagt aaacggttga agaagccata aagacctgca
     9241 ccaccccagc ctaaactcaa aatgaattta gcaaaagaaa ctaaagcaga gtaaaccgga
     9301 ggccatacga gcagtagcac tgcactgatc agcaacatga caaaggcagc caagattggc
     9361 actagacgct tgccgctaaa gaaagataaa gccatcggta attttgcttc gtggaaacga
     9421 ttataaagag ctgccgcaat caatccggca ataataccaa taaatacgtt actgatctga
     9481 gcaaaagatg gatcaactga ttctggtttg atattcaata aagtagctac tgaatcagtt
     9541 tttaaaacat ttactggaac ttcaaatgcg accaaacctg ccaaggcagc agcacctgat
     9601 ttgtccactg acatccctag tgctaaccca acggcaaaca gcaaaggtaa ttgacctaaa
     9661 actgcgttac cgccagcttg taaaaaatga gcgacaatgt cgttactata agatgcccac
     9721 cagtttccga tccctactag taaagcagca gcaggtaaga ccgcaactgg aagttgaagg
     9781 gaacgtccca ttcgttgtag atatgctttc aatttttttc ctcctcatga ttgttaacat
     9841 ttgtttgtac gattgcgctt cttaaacgct cgatatggat ggccaagtaa cccagttctt
     9901 ctttcgtgat cgcgagctgg tattctttgg tcagcaacac acggattttt ttagaaatct
     9961 tatagctttc tgggtatttt cgtttgacca tttctacgat ttccgtgtct aaaacagcat
    10021 attgttgcgc atgggttcgt tgtagaagca gtcgtaaatg attcaccagt cttgaataat
    10081 tcagtgaaaa tggttctgaa tggatatctg tttccaattc tgattccact aaatggatga
    10141 tgtcagatac aatcgtcact tcacggatac tgcgatggtt attgttccga ccgcttcgtc
    10201 cgctgtgaat atggattgcg atatatcctg cttcgtcata agtatatgga atcccaagct
    10261 cttttgtcag atactctact gcccattgag caatactgaa ttcatcactg taaagcactt
    10321 cgatttctct taaaagtttg ttgcggatga cgataccgtt attaatgttc tccgcagaaa
    10381 aagcaatgtg gtcggtcaaa gcgatcaata catgttcatt caacttttcc atcaaaacag
    10441 tttccgcatg atcaatgatc gtttctgctg caaataaaaa gcgttcatca atctgattaa
    10501 gtaaattttg caacttgatt tgaccttctg gttccatgac aaatatccgt tcgacatcac
    10561 gatcaaaaat caaatcattt ctttttctgt caaatccaat cccttttcca atagctactt
    10621 tttcctgtcc accgtcatct accaatactg cattttgatt aaagatcttt ttgattttca
    10681 tgcgcgcacc ctttttgaag atttggtttt cacgtttgct agaccagttg attgcggcca
    10741 aacaaaaata gacacgaaaa acctcctagg gaaaccatgt tcctcagaga tttttcatgc
    10801 ctagtatgat ctagtcacat gaaacgaatt attgttaacg taatcaattt tatatagaca
    10861 gttatgtttt gtcaattctt tttttataat tatagtaatt tactggaatt atcagaaata
    10921 aaaggaagaa ataaattaag aaacttatgt tatttaatcg ttggatcttt tgcttatctt
    10981 attcgttgtt aaaaagctga aagcgaagga aaaaaactct acttaactgt atttctccgt
    11041 taggacatct aaaaatttac gagcagcagg agaaagaggc tggtcttttt tccaaatgag
    11101 attcagtgaa gcttgtaatg gcggatcgag tgggatgaat attttttccg tcccataagt
    11161 attgatgatc ccatctagag ctaatgcacc aaaagtcccg ttatcgacca tcaaagaagc
    11221 attgtacaat aagttatatg ttccgacgat cgataactga tctatgctga aacctagcca
    11281 ttcagctagt tgactgtcca ctgacgtttg ccgggaaatg atcaaaggaa cagttttcag
    11341 ttcctgcgga tggatgacgg tttgctttgc taaaggatga tttcgatgta tcaaaagtcc
    11401 ccaacgatct tgggctggca gtcgaaatga ctggtatttt tgtttgtttg tcggttcgat
    11461 tacgagtcca aaatcaagta atccgctatc cagtttgtct atgatatcat ctgcattccc
    11521 gctatataaa tgaaactgga tatcaggatg cgccttgatc aacgaatgaa ggctatcaga
    11581 aatgaaagag attgctttag tttcgcctcc gccaatgtag atttctccag agatgattgc
    11641 gtcatttgtt aaattgttcg ccgttttttc taccaacgac aagatttctt ttccttttga
    11701 ataaagatat cgaccagaat ccgtcaactg tatctctcgg gagccccgag tgaaaagtgt
    11761 cacaccaagt tcttcttcta aatctttcag ttgttttgag agagtaggtt gcgtgatatg
    11821 cagagatttt gctgctcggc tgatattttt ttctctagct actgctaaaa aatacgtcag
    11881 tatacgaata tccatttttt ccctcctgtt tttaaaaaag cataccacta agtatagtta
    11941 aaaggaatag ttaagtatgt tttataagta ttagacatga taagccgtcc gttataaagt
    12001 aaggataaaa ggagggagca aaatgacaag cctcatcgaa aaacaaatcg tcaataaaga
    12061 gattcgagta acggatccgc tctttgaagt gatccatcag atccaagctg aaaacgaaga
    12121 gcctttagca gcgctgaata ctgggtacca tgaaccagca gatatccgca aacggctaga
    12181 aaatatcatt agtgacaaag tagatgatac ggtgactgtt ctgctgcctt tttatacaga
    12241 tttcggcaaa catattagta tagggaaaaa tgtctttatc aatcgtcaag tgatgttcgt
    12301 tgacttaggt ggcatctgtt tagaagattc agtattgatt ggtcctcgtg tcaacctcat
    12361 cacggtgaac catctaaccg atccaaaaga acgaagaggc ttgtcagtaa agccaatcca
    12421 catcaagaaa aatgcttgga tcggagcagg agcaactatt ctgccaggcg taacgattgg
    12481 cgagaatgcc attgttgcag cgaatgcaac tgttacgaaa gatgtcccag acaataccat
    12541 cgttgcagga atccctgcca aaatcgtcaa acctgtcgaa cggattattt gaacagtaag
    12601 atgaaataag agatagtgag tataagcaag agatttttca aaacaggagg agaatagaat
    12661 gaatgattta tcagaaaaag tgattgtcat catgggcgct tcaagcggaa tcggtgaagc
    12721 aacaacgaag ctattagcag aaaaaggggc taaactagtt atagctgcac gccgagaaga
    12781 tcgcctaaaa gcaataaaag aatctttacc agaagctgag ctatatattc aaacagcaga
    12841 tgtaagagat tttgcacaag tccaagcagt tatcgattta gcgatggaaa agttcggtcg
    12901 aatcgatgta ttgtacaata atgcaggaat catgccaacc gctccattag ttgaaggtca
    12961 ccgtgatgaa tggcaaaata tgcttgatat caacatcatg ggtgtgttaa atggaatttc
    13021 cgccgtactt ccaatcatgg aaaaacaaaa atctgggcat atcatttcaa ctgattctgt
    13081 ggcgggacat gttgtttatc ctgattccgc cgtttattgc ggaacaaaat ttgctgtccg
    13141 tgcaatcatg gaaggattac gtcaagagca acggcaaaat aacatcaaat ctacgatcat
    13201 ctctccagga gctgttcaaa ctgaattata tcaaaccatc agcgacaaaa aggctgctct
    13261 tgaacttcat gaagcgcaaa aagaatgggg attgacctcc gaagatatag cttctgctgt
    13321 agcatttgcg atagaaacgc cagaccggat gtctgtcagt aacatgatta tccgtccgac
    13381 gacacaggaa gtataatcca taacggataa tatccaaaga gaattacttg ttaaatagtt
    13441 attcaaacga ccatcaattt aaggcgtgaa tggagaagaa tattcttcat ttacgtcttt
    13501 ttatgtagaa aaagcaaatt tatcaaaaag aaacaaatgt tatttaaata tggacattga
    13561 cttgctttta cttttttagt tacactgtta tttaggaaaa gtttcataat tcaaaggaga
    13621 aaaaagatga aagcaaagaa tttattattt agcgttattc ttttaggaaa tctagcagtt
    13681 gccaacacaa cagtgctagc tgaagaatcg acagaaactg tcgcaactca gacatcgagt
    13741 tcttcagaaa caggaattga aaacgataca gagagtactt tgcagtcagc tactgtctca
    13801 gaaaatacaa tttcaaccga agaaccaaca actgaaacaa cagaacaaac acaaaccgaa
    13861 gacagtacat cagcagaagc tcagaacaat ccagagaatg cagaagaaat cacgaaccaa
    13921 ttgacagatg ggcaaacatt gacacatacc catcaagcag atttgtctga attcgaaaac
    13981 tatactggct atgcttttta tgctcgctca tctgctaatt ctcaacaagc gttcattgat
    14041 agtattgcct caactgcaca aagtttggca agtgccaatg acttatatgc atcagtcatg
    14101 attgctcaag ccatcgttga aagtggctgg gggaacagta ctttggcttc tgcccctaat
    14161 tacaatctat ttgggattaa aggaagctat aatggccaat ctgttacgat gcctacttcg
    14221 gaatatgtga atgggcaatg gattacggtg aatgccgctt ttagaaaata tccttcgtac
    14281 aaagaatcat tgcaagataa cgtaacggta ttgaagacga cttcttttca gccaggcgtt
    14341 tactactatt ccggtgcatg gaaaagcaat acgaactctt acaaagatgc tacggcttgg
    14401 ctaacaggtc gttatgccac tgcgcctaat tatggttcta cattgaacaa tgtaatcgaa
    14461 acctataatc tgacacagta tgatacagca ccaacttcaa cttctccgat gtatcgtctg
    14521 tataaccgtc atacaggaga acatttatat acattgaatg ccggagaaaa aaattatctg
    14581 ccaactgtag gctgggaata tgaagggatt gcttggcaag caccaaacag cggacaacct
    14641 gtctaccgtt tatacaatcc ttacagtgga gaccatcatt acacaatggc acaatctgag
    14701 atcaactctt taacaaaaat cggttggcgt tatgaaggtc taagttttta ttctggtggt
    14761 tccaagccaa tttatcgact atttaatcca aatgaaaaaa ctggtaccca tcactatacc
    14821 ttgagtgcta aggaaagaga ttggctaaca ccgatgggct ggagatatga aggaatagga
    14881 ttttatggtt attaaaaaga ggtagtgaaa aagctgccct gcatcaagta gtaagaacag
    14941 ccaaacaaaa acagattttc atgttttttg tttggttgaa cttattaccg cagatgcaag
    15001 tttttgaaca ccgtttactc aaaaaaggtt gtgacaacat cttgtcataa cttttttatt
    15061 actgtttaag catactgatc caatttctaa tattcgacgg gattcttgca aaaggcgatt
    15121 tcggaaattt ttggcggtgt aaaattttct actgcagaag agctatcttc tatagtttga
    15181 taagaatcaa taatcttggt tttagcgttg tgctttcttt atcatttttt ccgttcattc
    15241 tacaaaaaat ttcttttctc tatacttttc tattgagagc gtttttttta ttacacagct
    15301 gattctttga cttttttctt tttttattct attattgaca agtcaatcgt tgacgagtca
    15361 attattagaa aggaggaaat tatgtcttta gaagagatta gtcacctttt atatcaaatc
    15421 aaattagccg atcaaaaaat cgtccagatc tttgaagaaa atatcggtat aagtcttact
    15481 agatacgaga tcatgatgag actgaaagaa gaaaatccat tgctacaaag cagtttgcaa
    15541 gaaaagttgc agattgatca aggagcagtt actcgtcatc tgaaggtatt ggaagcaaat
    15601 ggctatgttt cgcgtcagag aaatcctcag aacaaccggg aagtgtatgt agcgctaaca
    15661 gaaaaagccc agaagaaaat cgagcattgt gaaatggacc ataaagatac aggttccgtg
    15721 ctatccccgg ctttcagcga tgtagaaatg aaacaatttc taacactttt agataaattg
    15781 aatacccaac tttgcaatat aacaacagaa acgaaagagg ttaataataa tggaacaaaa
    15841 aaatgatttt tcaacaatta tgactggtag aagatccgtt cgcgtatatg atgaaacata
    15901 caaaatccca cacgaagaaa tgctggaaat gatcaaagaa gcgacgactg caccttcttc
    15961 tgtcaatatg caaccatggc gctttgtggt cgctgaaagc gaggaagcaa aagaaatctt
    16021 gcgtcctttg atccgtttca atacgagaca aaatgatact tcttctgcaa tggtcatgat
    16081 ttttggcgat atgcaatgtt acgaatatgg cgaagaaatc tataaccaag cttacgaatc
    16141 agggaagatg ccaaaagaag tcagagatca acaattagct gcgatcattc cttattacaa
    16201 gagtttttca agagaagaaa tgaacgatgt agtcaaagtc gattctagct tagcggctat
    16261 gcaattcatg ttagttgcgc gtgatcatgg ctatgaaaca aatccaattg gtggattcga
    16321 agcagatcag ttggcagagg catttggttt ggataaagac cgttatgttc cagttattat
    16381 tttatcaata ggaaaagcgg tagaagaagg ttacgagtct gttcgtttag caccagaaaa
    16441 gatcactacg ttcgaataaa aggttttcag ttactattca gtagctattg ctttttttga
    16501 atcgtgtgtg gtaacctaat aaaaaaggag gtcggaaaaa tgactgcatc gatgaaatta
    16561 cgtttcgaac ttttgaataa caactaaata aattcaaaag acatgtataa acaacaattt
    16621 catggatgga gtatgtccat tttttcgaac gacagtagaa taggtgaaag ctatgacaga
    16681 tttggtgatt tagtcatagt tatttcgttc agagtaggaa aagatgtgat gcagtttttt
    16741 tgcaaacaga tttttctgct cttttttagt atcgataatt gttgttttac atgaaatagg
    16801 agtgtaaaac atatggaaaa tttagcagta aatataacaa atctacaagt cagctttggc
    16861 aaccagctag aactatctat tgattctctt cgtgtctatc agcaagatcg tatagggatc
    16921 ataggagaaa atggtgtcgg taaatcgact ttgctcaaac taatagccgg tgaacttttt
    16981 cccgatcatg ggaaaatcca aacagagatc accttcaact acctgcctca attaacctat
    17041 ctcgctgaag caaaggacct aaatttggaa ttagccagtc atttccagtt aagactggaa
    17101 gaaacttcag agcggaaatg gagcggaggg gaagaacgaa agatcgagtt gatacgtctt
    17161 ctttcttctt atgaacaagg gatgcttcta gacgagccaa caacccatct agatagaaaa
    17221 agtattgatc gactgattga agagcttcgt tattattatg gtacgctggt ttttgttagt
    17281 catgatcgct attttctaga tgagctagca tcgaaaatct gggaagtaaa agacggagaa
    17341 atccgagagt tttcggggaa ttatagtgcc tatctcactc aaaaggaatt ggagaaaaag
    17401 actcagctac gagaagcaga gtcgatcatg aaagagaaaa aacgattgga aaaatcgatc
    17461 caagaaaaga aaaaacaagc ggaaaagttg gaaaaagtgt ccagtaaaaa gaaaaagcaa
    17521 caaatcagac cggatcggtt gtcttcctct aaacaaaaag acagtgtaca aaaagccatc
    17581 caaaagaatg cgaaaacatt agagagaaga ctccaaaaaa taggagaaac aaccaagccg
    17641 cagcagatga aacaaatccg ttttccagta ccaaaatctc ttgaactcca cagccgctat
    17701 cccatcatgg gacaaaatgt ccaattggaa agaagtggga gaacattact ggtaaatggt
    17761 gattttcagt tttctttagg taaaaaaatc gcgattgtcg gtgaaaatgg ttcaggtaag
    17821 acaaccttat tagaacatat ccgcaaacaa ggagaaggaa tccttctctc tccgaaagta
    17881 agctttcaag tatatcagca aaagggttat caaatgacat ctgaagaatc catcattcgt
    17941 tttgtcatga gacaaacaga gttttcagaa tcgcttgtcc gtagtttgct gaatcactta
    18001 gggtttgctc aggaaacttt gacgaaaccg ttatgtacat taagtggggg agaagcgacc
    18061 cgtctgacga ttgctttgct ttttactaag ccaagtaatg tgttgctgtt agatgaaccg
    18121 actaatttta ttgatatggc aacgatcgaa gctttagaga agctaatgca aatatatccg
    18181 ggaacgattt tgtttacttc tcatgattct tactttgtcg agcgtacggc tgatgaagtt
    18241 tatgaaataa aagggcagaa aataaaaaaa gtacttacga gaaattttta aatgtgctac
    18301 gatgattttc gtgacaaatt taaaggagtg gtacagattg cctacaaata aaaaagaagg
    18361 aataattttt acaacaatga tgtgtttttt gatggtcttg gggatgagcg cgtataattt
    18421 atggctgcat caagatcttc atttatcaag tttgttgatt ggtctagtgc ctggattcgt
    18481 ggtggctttc attttggatg tcttcatcat cggtgtgctg gcgaaaaaaa ttgcctttaa
    18541 actaccagta aatccagaaa gcaaggtcca aatgatctta actatttcat gcttgatggt
    18601 tttaggaatg gtgacatgta tgtctttatt cggtgtgatc atggaacaag gaatacctga
    18661 tcaattattc tctgcttata tgaatgcttg gaaaatgaat gtgattgccg ctttaccgtt
    18721 gcagttgatc atcgttggcc cgctttcaag aagtgtgcta aaagcaattc aaagtaatga
    18781 atgatggaac actgttattt gaaaaaaggc cttggcatgc tttttgtcat agtctcttga
    18841 atgcagttct agaaaaatag tgatccgaac gataggtttt ggcctgtcgt tcggatcgct
    18901 ttttcttttc caactgtatt gctcttttat tgaagataat ttgctcttca atagatattt
    18961 ctagaaaggt tctgtttatc ccttactcaa aaaactgctg catttgttct cttacttgct
    19021 gacaccattg gagttcctgc tgacaaagca taattctgcg ctggatcaca acataacgtc
    19081 caaattcttg tttcttgtct agcgagaggt cattcaattt tttttgattt ttttgcatga
    19141 ttttgttcag ctgttgttct ctagtgaata gcagtccagt agctgttgtt ttatcgagcg
    19201 tcgaaaaagc atagattttt gctagaaatt catctttttg cgtaggagcg ggagtttcct
    19261 ctttgatcca ttcctctaca agagcacgtc cttcatcagt caagtggtag atcttttttt
    19321 gagagtgctc gtctttgtcc aaaacggtca cataaccctg ctggtctaac ttggcaagcg
    19381 ttgtatagat ctgactgtga tgggcttccc aaaaaattgt gatcagctgc ttcaattcat
    19441 acccagaacg tggtttggat aaaagcacgc ataataatat ataggctaaa gtattcataa
    19501 aaatcctacc tctcgtctta ttttctttat tatatcacag taaagtttcc acgttttaca
    19561 tgtgcatatt tacatgtaaa ataaatcatg tatgatgcta atagagagaa agggatggaa
    19621 atattggaga atcaaataga tatacaagaa caaacaaatg aagaaacaaa ggggaaaagt
    19681 tcagcgctag ttgtagcagc tacctgtatg atcgctttta tgggagtggg tctagtagac
    19741 ccaatcttaa aaacgattgc tgcacagtta aacgctacgc cagctgaaac gaccctgtta
    19801 tttactagtt acatgttagt cactggtgtg gtgatgctat ttacaggttt cttatctagt
    19861 cggatcggtt tgaaaaaaca ttgatgacag gattgtttat catcgttgta ttcgctggtc
    19921 ttggcggatt atctgggaat atcggaatgc tgattgggtt acgtgccggc tggggtgtcg
    19981 ggaatgcttt atttgtctct actgctttag caacgatcgt cagtatttta gttggtaaca
    20041 cagaaaaagc aatcatgatg tatgaagcag cacttggctg cggaatggct gttggccctc
    20101 tagtcggtgg ggtcttgggc agtggttcat ggcgttatcc attttttggg gtagcatttt
    20161 tgatgttttg cggttttata gccctagcgg tacttttacc tgaaactgag aaaccaaaga
    20221 gaaaagtagg cctttgggaa ggtgtcaaag cgttaagcaa tcataaactt cgttcagttg
    20281 gactaatcgc attgttatat aatttcggat tctttacttt acttgcgtat gcgccatttt
    20341 tattgattgg ctattctgaa ttagaagtcg gttttgtatt ttttggctgg ggtgtgcttc
    20401 tggctattgc ttcgattttc gttgcaccgg ctcttgagcg gaagtttacg acttatcgga
    20461 caattttagc agcatttgtc ttattcatta tttgtttgct gttattagga gctggaactt
    20521 ctatgccagt ccttgtaccg atcagcattg tgttagcagg atttttccaa ggaattatca
    20581 atacactgtt gacgacgatt gctatggaaa taccaggtct gcaacgaaac gttgcttcgt
    20641 ccagctatag ttttgtccgc tttttcggtg gagctttagc accatttatt gcaggtgtga
    20701 ttggtgaaca tattggtgaa agctatgcgt tttattttgc aggtgccatt gttcttatca
    20761 gtgtatgttg tatcatttat caccgtaatt attttgctac aaatgaagaa gaataagaaa
    20821 tcagaggttg tgaaaaagct gtcctgcatc aagtcataag aa
//
LOCUS       DACGLW010000205        17409 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.guided.064, whole genome shotgun sequence.
ACCESSION   DACGLW010000205 DACGLW010000000
VERSION     DACGLW010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17409
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.guided.064"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            196..1455
                     /locus_tag="IXT53_003001"
     CDS             196..1455
                     /locus_tag="IXT53_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HAQ4906740.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            1461..2042
                     /locus_tag="IXT53_003002"
     CDS             1461..2042
                     /locus_tag="IXT53_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HAQ4906741.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(2124..2999)
                     /locus_tag="IXT53_003003"
     CDS             complement(2124..2999)
                     /locus_tag="IXT53_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HAQ4906742.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            3147..3479
                     /locus_tag="IXT53_003004"
     CDS             3147..3479
                     /locus_tag="IXT53_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HAQ4906743.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            3591..4799
                     /locus_tag="IXT53_003005"
     CDS             3591..4799
                     /locus_tag="IXT53_003005"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HAQ4906744.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            4953..5408
                     /locus_tag="IXT53_003006"
     CDS             4953..5408
                     /locus_tag="IXT53_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906745.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            5770..6045
                     /locus_tag="IXT53_003007"
     CDS             5770..6045
                     /locus_tag="IXT53_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906746.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            6058..8136
                     /locus_tag="IXT53_003008"
     CDS             6058..8136
                     /locus_tag="IXT53_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HAQ4906747.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            8296..10182
                     /locus_tag="IXT53_003009"
     CDS             8296..10182
                     /locus_tag="IXT53_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HAQ4906748.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            10194..11141
                     /locus_tag="IXT53_003010"
     CDS             10194..11141
                     /locus_tag="IXT53_003010"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HAQ4906749.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            11161..11688
                     /locus_tag="IXT53_003011"
     CDS             11161..11688
                     /locus_tag="IXT53_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HAQ4906750.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(11751..12404)
                     /locus_tag="IXT53_003012"
     CDS             complement(11751..12404)
                     /locus_tag="IXT53_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HAQ4906751.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            12536..13378
                     /locus_tag="IXT53_003013"
     CDS             12536..13378
                     /locus_tag="IXT53_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HAQ4906752.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            13536..14432
                     /locus_tag="IXT53_003014"
     CDS             13536..14432
                     /locus_tag="IXT53_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HAQ4906753.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            14435..15148
                     /locus_tag="IXT53_003015"
     CDS             14435..15148
                     /locus_tag="IXT53_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HAQ4906754.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            15168..15686
                     /gene="msrA"
                     /locus_tag="IXT53_003016"
     CDS             15168..15686
                     /gene="msrA"
                     /locus_tag="IXT53_003016"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HAQ4906755.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            15683..15910
                     /locus_tag="IXT53_003017"
     CDS             15683..15910
                     /locus_tag="IXT53_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HAQ4906756.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            16062..16613
                     /gene="lepB"
                     /locus_tag="IXT53_003018"
     CDS             16062..16613
                     /gene="lepB"
                     /locus_tag="IXT53_003018"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HAQ4906757.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(16696..17238)
                     /locus_tag="IXT53_003019"
     CDS             complement(16696..17238)
                     /locus_tag="IXT53_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HAQ4906758.1"
                     /translation="MNTLIAKDVFRLPEMNARLLSTSALLLALDVILYKIAIGPAYFQ
                     ITFGFLSMALMGYLYGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLF
                     LYHKKINLPSVIAAQLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQI
                     MVVYSFIKTLEARKVFKRAV"
BASE COUNT         6084 a         2950 c         3573 g         4802 t
ORIGIN      
        1 tcaccatggg tggctaactt aaacttgaaa tttacgattt ggaaaaagtt ctttttttgg
       61 cagaaaaaag caaaaaaata cttgttttag aaaaaatagg tataatagac ggataccaac
      121 atccaaacga aaaagaatta ttgagaacaa tgacggtatt tcaacaaatc gtcagtgaga
      181 taagagggaa ttgaaatgaa tacaaatagc gaaaaaatgt tgcaagcttt atcagatgaa
      241 gacttgtcac aagcacaaat ctttttagaa aaagcattaa aagaagaccc agcagatata
      301 ttagctgaac taggcgaaga attgctggct attggctttt tgatcgaagc aaaacagatt
      361 tttgaacaac tgatcaacca atacccagaa aatgacggac tgaacatccc gttagcagaa
      421 attgcaatcg aagataatga gatcgactta gcttttgaat acctggaaaa agtggataag
      481 caaagtgatt actatccgca aagcttattg gcgatagctg atttgtacca agttatcgga
      541 atccctgaag tgagtgaagc aaaattgaaa gaagcagctg ggttgcttcc tgatgaacca
      601 ttgatccagt ttgcgctagc tgaattgtat ttttcagttg atcgtttcaa agaggcagca
      661 gtggtttatg aaacattgtt ggcaagcggg ataaatgaaa tttcaggaat ctcgatgcag
      721 gaacgtttag gacaatcctt aagtatgcaa ggagaattcg aagcagctat cgtaccttta
      781 gaagaagcac tgaaagaaga aaggacagat gatcgactgt tccagctagc ttttacgaat
      841 cttcaattga aagaaaatca gcaggcaatc aactatctgg aagaacttcg agaagttaat
      901 ccgcattatc aatcgttata tctatatcta ggacaagctc ttcaagaaga agaaatgatt
      961 gaagaagcac aaacagtgtt agaagaagga atcaaagaaa atccttacca agtcgaactt
     1021 tatcacctgg cctcggaaaa tgctttccgt ctgcatgata gacagcgagc agaagaactt
     1081 ttactgaaag ctttagaagt aggagacaaa caagatgaaa cattacttac tttaagtaat
     1141 ctttacttgg atgatgaaag atatgaagat gtgatacaag tgatcaatca gatggaagaa
     1201 actgctaatc cttatgcaga atggaaccta gcccatgctt ataatgaatt agaagacttt
     1261 accgttgcag ctgtacacta tgaacaggct tatcatgaac tatcacatga accagatttc
     1321 ttgaaagagt atgccttttt cttaagagaa gaagggcagc tgaaaagagc gcaagaatta
     1381 ttgacttatt atttaacact tgaaccagga gatatggagg cactgtcatt attagatgac
     1441 ctaacagaga ggtgagaaaa atgctaattg atgtacggga aaagaagaat tttttaacgt
     1501 ggatggtcaa tcatgtatct tttagccgaa gagaagtttt ttggatcttg aattatcttg
     1561 ccaatcatga agcgatttta agcaatgtcc actttgttga aggggcgaat tttgcggatc
     1621 gaggactgca gatcagtgat ctatctgttg aaggggaacc catgaaattg tttttgcagg
     1681 gaaaagaatt tacggacaca gaccagattt ttcatgaaat ccgtttgaat tggaaaaagc
     1741 ctttatatgt ggaatgtata ttcgaaaatg cttggcagac aagagaatat ctatctatct
     1801 tagaagacaa tccattttct tcttgggatt cttcagtcag tgaagaagat actgaattaa
     1861 ttgaacgata ttttcaagaa aaagaagaag aggcaaaact aaaaatgctt tatgctcaaa
     1921 tcgatcaagc actagaagat ggagataggg atgctttttt gaaattatct gatgaagtta
     1981 accgaaagat ccttttgcag acacaaaaga aaagtaaccg ggtaagtgaa cataacaatt
     2041 agttaaaaat agtatagtac taaaaaagac aaaggtcagc tggatgtgga atctttcctc
     2101 atagcctatc tttgtctttt ttcttatagt tttttcagta atcttgtctg tggcttcaaa
     2161 taagtaaagc cttgcccttc cacttcatgc acattgatga ttgaaaggaa agctttttcg
     2221 tcaatctcgt ggacgatcct cttagcttca gttaattcac tagagctaac gaccatatag
     2281 atgatttttt ttgaattacc agagaatgcg ccttctccag aaaggaaagt cacacctctt
     2341 tgcagaccgg tcatcagtag tggtgcaatc tcttcgcttt tgttggagac gacaagtaga
     2401 ccttttgcag aatatccgcc atctaagatc gaatcaatca cacgactgaa aacatacgaa
     2461 acaatcaacg tatacatcat acgtttcaag tcgatatagg ttaatgaagc aagtaagacg
     2521 atgatatcaa atgccaatag ggaacgcccc atagtgatgc cgtaattttg ttctaaaatc
     2581 cgagcgataa tatcactgcc gcctgttgtt ccacccatgc ggtagacgat cccgcttcct
     2641 acacctgcaa ctaaacccgc aagtagagaa acgatcagta gatcatgctg taaattgatt
     2701 tctagtggaa ccctttgcca gatccataaa aagaatgaca aagaaagtgt acctagaaca
     2761 gtataggcca aggaacgttt accgaggatc tttcctccta gtaaaatcaa tgggatgttg
     2821 attaataaag tcgtataagc tggatcaata ccaaataaag ctcttaagat caaggtgatc
     2881 cctgagactc ccccttctgc taaatgatta gcgatgttca agtaaacaag accaaatgca
     2941 taaatacttg ttcctataat gataaaaaga acttctttta ctgtttttcg tttgaccata
     3001 tgaattccag ctttcaatta tgtattttca cagaatttcc atttagaaat tccttcctaa
     3061 atgtagcatg gaaattcgat tttctcaatt gctcttatct aggatttgcg ttaaagtttt
     3121 ggtaagatag ttttggaggg aaaaagatgg ataaaaaacg ttctttatac agtatgcagc
     3181 aggaagtgga tgaatatatc cagcaattca aggtggggta tttctctcca ttagctcaga
     3241 tggctcgatt aactgaagaa gtaggtgaat tggcaagaga agtcaaccac acttatggag
     3301 aaaaaagcaa aaaggcttct gaaccagtta actcggtagc agaagaactt ggtgatgttt
     3361 tattcgttac gatgatcatg gctaattcat tgaatatcga cttaactgaa gtttttgaaa
     3421 aaaacatgga aaaattcaat caaagagacc ataatcgctt tgagcgtaag gacgagtaaa
     3481 caagatgaag tggtcctggc cttgaagact ctgaaacgga cttgtcaaac agtcaccatg
     3541 caactgagtc tttatctcag gacaagaaac acgcaggaaa ggttggaaaa atgaaattaa
     3601 aacaattacc tctggaatac caaaaggcaa ttccagttct gaaaaaatta gagaatgcag
     3661 gatatgaagc ttattttgta ggtggaagtg ttcgtgatat cttgctgaac cagccaatcc
     3721 atgatgtgga tatcgcaaca agcgcatttc cagaagaaat caaacagctt tttccaaaaa
     3781 cgatagatat tgggatcgaa catggaactg tgttggtatt agaaaatgat gaacaatatg
     3841 agatcaccac atttcggacg gaatcaactt atcaggacta ccgtagacca gatcatgtcg
     3901 aattcgttcg ttcgttggca gaagatttga aaagaagaga ttttacgatc aatgcgtttg
     3961 ctttgaaaga agatggagaa atcatcgatt tgttcgatgg tctgcaagat ctagaagaac
     4021 agatcttgcg agctgtaggc aatcctcatg agcggttcca tgaagatgcc ttacgaatga
     4081 tgcgcgggtt gcgttttgtc agtcaactag ggttcaaatt agaggaagaa accttctacg
     4141 ccatcaaaga aaaccacgag ctattaggta aaatatctgt agaacgtata aatgtggaat
     4201 tcatcaagat gctcttggga aatgaacggg ccgcgggaat ccatgcattt attgaaacag
     4261 agtgctacat ttgctgtccc ggtttgaaag aagaaggcga agcactttta cgttttgctg
     4321 aattaccatc cgtcccattg aaagaagaga gccaagcatg ggcattattg atcagccaac
     4381 tggatctaaa agaaaatgag attcgttcct ttttgaaatc gtggaagtgt tctaaccaaa
     4441 tgattaaaga cgttcaagca cttgtccaag gactacgaca acgagaaact ggagctttgg
     4501 aaccattcga attgtacttc ttaggcaaaa aacatgcact gcaagtagaa gaattgatga
     4561 tctattttga cgaaacacct cagatggatg ccgtaggaga atcctatgat cgtttaccta
     4621 tcaagtcatt aagcgaatta gcagtagatg gtaaaatctt gctagaagaa actggaaaaa
     4681 aacctggtaa gtgggttagt gaagctttgc aattagctga aaaagccgtc gtagaacaaa
     4741 gaataaaaaa tgataaaaaa caggttttgg attttttatc gaaagaaaaa ttgatttaaa
     4801 aaacactaga ctgtatctgc tcagctttgt tggctactta aagtgggcag atacgtcatt
     4861 agaagaaaaa acataagatg tgaccactta cataaatttg tgctaaactg aggtagtaca
     4921 agaataaatt acatcataaa ggagtctttt taatgagtaa agaaatgaca gcgctaaaat
     4981 tttattttag aaatggagaa acctggacga tcaaccgccg tcatatcggc gatctttgga
     5041 tcaaacaaat cacaacaagt tttggacgaa tcaatggcag tgaatttgtt gaaatccatc
     5101 cgtgtgctgg atttaaaatc gaaatcttcc atgaagggga cgcagttgcg acacatgata
     5161 tcaacctagg cggactggaa atgggcatgt ttaatcgcgc attgaaatac gaagatatcg
     5221 agcgaatgga aatcctttac cgaaatggca cacctgatct tgtgtacttc ccatatctag
     5281 ataaaggaac agaaggatta gacaatcaat atcaatcaac aaaaatcagc gaaaaaacag
     5341 gtaacttgta tatcgtgatc aatccagatc aacgtgtaga agatgtatac ggggaatttt
     5401 tcgaataaat cgatttttac gcaaaaatga ggctgtgaaa gagatgtgtc ttattactac
     5461 tgataggctg tctcttccac accgtttatt cagaataagg gactgtgaca agatttttgt
     5521 cgcagcccca tttttttgtt gtgataaaag aacaagaaag gcaggaatgg gaaagtaaac
     5581 atccttttgt cagaaacttt tatctttctt aagttttact tgatatctga tactatttgg
     5641 aatagaatga ggcgttgaaa aatttatgga aatttctaac tagcaataaa cgatgaaaca
     5701 atgaaatatt tttcatccaa aaagaacttt gctataatga tggaagtttg tgtataggag
     5761 atctggaaaa tgagagagag ttttgaacaa caaaaaaaac tgttgcatga tcgctatggc
     5821 gccctttcga tggatgaccg ccgtcaaata ctttgtaagt tgcgtaaaag aaatatattg
     5881 atgtatcgtc agttggaacg tctgaaacat gatctgcttc gtttggagtc aaaacgtgta
     5941 cagtgtgaat tagaggggaa ccaaacacag gttgaagtag tcgaaaccaa aattttaaag
     6001 aaaaaagaac aatttttaaa aatgcttaca caaaataaaa aataaagggg atggacgatg
     6061 gaatttgctg agttagttat tgtatttgca gtaacgatca ccttatcgaa catagctagc
     6121 cgcattttgc caatgattcc agctccactg atccaaattt tccttggagt ggtattaggt
     6181 ctgacagagt ggggacaatc aatcgatttt gaacctgaat tgttcttagt tatgattatt
     6241 gcaccattgt tatttagaga aggagaaaaa gcagatattt cttcgattct caaaaatttc
     6301 gggacaattt tgttcttggc atttggagga gtcattttga cccttgtcgg ggtaggtgcg
     6361 acgttatcat tcctgttgcc aagtgtccca cttgctgcct gctttgcttt tggagcagcg
     6421 ttaggtccga cagatgcggt agcagtcagt tctctttctg gacgagtgaa tatccctaaa
     6481 aaggcgatgc atattttgga aggagaaggt cttttgaatg atgcatctgg tgttactgcc
     6541 ttccagtttg ctcttggtgc gctgatcacc ggatcttttt cagcagtaaa cgcggggatg
     6601 agtctagtag tttctagtat tgggggagct ttgatcggct ttttgcttgt ctggttcaaa
     6661 caaaaaataa ttcatttgat cgaaaaagca tcagcacaag atgtcacagg gtatctgctg
     6721 atcgaacttt tgcttccttt tctagcctat gtattggcag aggcatttga tgtatctgga
     6781 atcattgctg cagtggcagc aggtattctc caagcttctg gttttaggaa aatctctgtg
     6841 tttgatgcag aattgtcgag cttgtcccac agtacatgga caactattgc ttttacactg
     6901 aatgcattag tatttatatt tttaggaatc gaattgacgc aagtgttctc gccagtatgg
     6961 ggagacggtt tatatccaaa cggtctttta ttagcgatta tcgtattaat ctctgtcatg
     7021 ctttttgtga tcagattcat ttctatcagt cttttttatg tattcaaaga tggcagtaaa
     7081 aagttcaaaa aacagttgaa cgagatcttg atcttaacat ttggcggtgt aaagggaaca
     7141 gtcagtttgg ctacgatttt tattttgcca ttttcaatca ataatatgat gttttaccag
     7201 cggtctttgc ttttgtttct gacggcgggt gtcatcttag tgactttggt catagggatc
     7261 attgtcttgc ctatgctgac ggaaacagag gaagcgaaaa gcacggattt gaatgctttg
     7321 atgattttag aagaggtcgt tgaagttttg cgaaaagaaa taaaggaaat tgatcgtaat
     7381 acaaaagaat ttttagccac tgaagcagtg atagaaaatt atcaagaacg gatacgtgat
     7441 ctgtacttgg aagatttaac cgatgatgaa aagcaagaag tacaagaaat acaagcactg
     7501 attttgtcga tcgaacggga tggattggat gaaagttatc gttcgggcaa gctttcttct
     7561 aatggttatc gtttttactc ccgttttctt tctcgattcg aacattctat cacaagccag
     7621 attttgtctt ttatcggctt ttggttcatt gttgtccgta ggttaatgcg aatcgttctc
     7681 catccgaaaa tgttttggca aagaagatat gctgatcgtc agacatttat tagtgatgaa
     7741 gatatccaag aaatcagaga gacatatcaa aaaaatactc agttgattat tgaaagttta
     7801 gataacttga ctgatattta tgatgaaacg atggttcaat tttttatcca gcaacgcaaa
     7861 cttgaaggaa taaaaatgaa ctcggggaat ttgatttctt cgtggatgat ccagcaagat
     7921 agtctgttta cgaaaaaaat gctaagaggt tattatcttg aaagaaaaat cattgatgaa
     7981 tacgaagttg ctgaaaaaat cacgacgttt tctgcgaaca gctatcgcag gaatatcaat
     8041 ttgttagaat cttatacgat gaacaaacca tctgataact tttctttccg atttgcgttt
     8101 cgagcaaagt caaaaaaaac gagtccaata aaataaaaag gtaaaaattt tcaagtttta
     8161 taaaaaacaa aacatatcag aacaggatcg aatcggtcct gttctttttt tgagaacaca
     8221 aaaagaacaa ataccaaaaa aattttcctg aagttcacta tttttatgat acaatatcca
     8281 agataaggag ggaacatgtt gaaagaatta aaagtaactg acctcaaaaa aacttatggt
     8341 gaaaaagatt tgtttgacca gatttctttt ttggtccacg aaaaagaccg tatcgggttg
     8401 attgggacga acgggacggg taaaacaagt ctcttaaata ttattgccgg tatcgatagc
     8461 agcgacggtg atcggcaaac agtgttttat ccaactgatt accgtattgg ttatctttct
     8521 caagcagctg aattttccga tgaattaact gttctccaag cagtctttca aggaaacagc
     8581 cccttgatcc aaactgttcg agcatatgag gaagcattga tcgaactagg ggaaaacgga
     8641 gaagaccctg atgtgcaaaa acgttatgct aaagcagaag aacaaatgaa taaagaagat
     8701 gcttggacta cagataccaa cgcaaagatt attttgcaaa aattaggaat cagtgaatta
     8761 gataaaaaaa tcagtacttt atcaggtggt cagaaaaaac gagttagtct ggcgcaagtg
     8821 ttgattgatg aacccgattt actattacta gacgaaccaa caaaccattt agattatgaa
     8881 gcgatcgagt ggttggaaaa ctatttgaaa caataccgtg gcgcactatt gatggttacc
     8941 cacgatcgtt attttctaga tagagtagca aaccgaatct tcgaactttc atttggaaaa
     9001 ttatacgagt ataaaggaaa ttatgaagct tacgtcttag aaaaagcaga acgtgatcgt
     9061 gtagaagtag aacaagaaga aaagcgcaag cggctttata aacaggaatt agcttggatg
     9121 agggctggcg tacaggcacg tggcaccaag cagcaagcac ggatcaaccg tttcgaagat
     9181 ctaaaggaga acttgtacca agtaaaccaa gaagatgatt tagagctgaa cctagcgaca
     9241 caacgtttag ggaaaaaagt actggaaatc aaagatggca gttaccgtat aaatgaccaa
     9301 acactgctag aacatttaga tttattgatt caatctcggg aaagactagg tattactggt
     9361 aaaaatggtg cgggaaaatc gacattgctt aatattctag ctggcagaat tcccctggat
     9421 agcggtacaa tgtctatcgg tgaaacggtt catcttgcgt attatacaca agaaaatgaa
     9481 gaaatggcac cagataaacg gatgattgct tacttacaag aagctgctga agaagcaaaa
     9541 acagcagatg gcagtcaaat cggagtagca gaattacttg aacgattttt atttccaaga
     9601 tttatgcatg ggacactgat aggcaaactt tcaggcggag aaaaacgccg gttatttttg
     9661 ttgaaactgc tgattcagca acctaatgtt ttattattgg atgaaccgac caatgatttg
     9721 gacatagcaa cattgacgat tttggaagat tattttagaa gttttccagg tgctgtgatc
     9781 actgtttccc atgaccgtta tttcttggac aaggtagcag ataagttatt agtattccaa
     9841 ggaaacggaa aacaagaact ttattacgga aacatgtcca gttatttact gaagcaaaaa
     9901 gagacccagc agcctgctga aaaagcgaaa ccaaaaatac agagcaagga acctgctgaa
     9961 aagaagaaat tatcttacat ggaacaaaaa gaatgggaaa cgattgaaga tgagattgca
    10021 gaacttgaag aaaaaatctc attgctccaa gaagagatga atcatcaagg agataatttc
    10081 actcgattac aagaattgca aaacgacgta tctgaaacag aagcacaact tgaagaaaaa
    10141 atggcacggt gggaatactt gagtgaatgg gtagaagatt aggaggaaaa aggatggaac
    10201 aagcgtatct tgacttagga agaaaattgt tggaagaagg gcatcgtaaa gaagacagaa
    10261 caggcacagg cacattaagc ttattcggat accaaatgcg gtttgatctg caaaaagggt
    10321 ttcctttgtt aacaacgaaa cgagtacctt ttggattgat caaaagcgaa ttgctttggt
    10381 ttttaaaagg agatacgaat atccgctatc tattacaaaa taaaaaccat atttgggatg
    10441 aatgggcatt tgaacgctac atcaaaagtc cagactataa tggaccagat atgactgatt
    10501 ttggccatag atgtttgaca gatcctgaat tcaatgaaga atataaaaag cagagcaagc
    10561 ttttttgcga gaagatccta accgatgatt cctttgctga aacatacggc gatcttggac
    10621 acatctacgg ctatcaatgg cgtcactggg aaacaaaaga cggtggcttt atcgatcaaa
    10681 tcaaacaagt gatcgaagca atcaaaaaga ctcctgattc tagaagattg atcgtttctg
    10741 catggaatcc ggaagatgtt cctagcatgg ctttgcctcc ttgtcatacg atgtttcaat
    10801 tttatgttca agagggacgg ctaagctgcc aattgtatca acgcagtggg gatgtgtttc
    10861 taggcgttcc gttcaacatt gcaagttatg cgttgctgac tcacctaatt gcacatgaaa
    10921 caggtttagc ggttggcgaa tttgttcaca cattaggaga tgctcatctt tatatcaatc
    10981 atatcgaaca aatgaaagaa caattgagcc gagaaatacg cgcatttccg actttagttt
    11041 tgaatcagga aaagcagtca gtatttgatt ttgagatgga agatatccaa ttagaaggat
    11101 atgacccaca tccggcgatc aaagctccta tcgctgttta gaatcagaaa ggatgagaaa
    11161 atgttagctg caatttgggc acaggatgaa aacggattga ttggaaaaga agaccgtctt
    11221 ccttggcgtt tgccaaatga tctgaaattc ttcaaacaaa tgacagaagc aaatacacta
    11281 gtgatgggac gaaaaacatt cgaagggatg ggtagccgtc cgttgccgaa tcgtcagacg
    11341 attgttttga cgagagattc ttcctataaa gcagaaggcg tgcatgtcat gcatagtgtg
    11401 gaagacgtgc ttgctctgga aaaagaaaca gacggtatct ttttcatcgc tggagggtca
    11461 gctgtttatc aagaatttct gccattttgt acgatattat accgtacagt catccatcat
    11521 gtattcgatg gagatgctta ttttccacct gttgactggt ctgattggtc actgatcaat
    11581 actagtcaag gagaaatgga tgaaaaaaat cgttatccgc atcaatttga aacgtatcag
    11641 cgtaatgaga atcctgtaaa aagcgggaca ggcgaattga cgatataaaa aagctatgga
    11701 tagactagct gttatttaga gctgtcccat ccatagcttt atttttttta ttaagtaaag
    11761 aataaaacag agaagtacat aaaacctgca cctaacataa caaacaaatg ccaaacaaca
    11821 tgcatatagc ggatagaatt caagctataa aagaatgcgc ctatcgtgta agaaagcccg
    11881 cctgctacca tcaaaataac accagtcatt ccaagagagt gataaagagg aacagcagcc
    11941 acgacacaga gccaccccat aaaaatatag atgatcgtag atacttttga tactctctcc
    12001 cgtttatgca acgtcaacgc tttatatata atgccaagaa tcgcaaacaa ccagattaat
    12061 acaaataaag tccaacctag ccatcctcgg atactcagca aacaaaaagg agtataactt
    12121 cctgcaatca aaagaaaaat cgaactatga tcgaatactt ggaacacctt ttttgctttt
    12181 gtaaaaatca atgaatgaaa caaagtagac gttaaaaata aaagaatcat catcgatcca
    12241 tagatagcat aagagacaat atggagggag gaatgcaagt gggcaccttt tactaaaaga
    12301 accactagtc ccgcgatact caatccagtc ccaattccat gggtgactgc attaaacact
    12361 tcatttaata tttcatacgt tcgattaaat ttcgtttttt ccatgaaaac ggctccttta
    12421 ctaggtgtaa ttaatgaaaa atcattattt gtctgttata ctaaaaatag aaattgtctt
    12481 acatgtagtt taacatgaat aaggcatctt gttaaagaaa gagagagaga aaataatgac
    12541 aaatgtaaaa atcgtaacag attcttcatg tacgatggaa aaaaatgtac gcgatgcttt
    12601 aggcattcgt gtcatgccgc tatccatcat gatcgacgga acagtatatc ccgatgatga
    12661 ccaactagac ggcgaaagat ttatggaaat gatggcgaaa gcaaaaaatc tgcccaaaac
    12721 aagccaacca ccaattggag aattcatctc gatgtatgat gagctaggag aagacggcag
    12781 tgaagtgatt tcgatccata tgacaaaagg cttgagtgga acagtagaag cagcaagaca
    12841 agccagtaat ctaacaaaaa cgaaagtaac ggttatcgac agtgatacca ctgaccaagg
    12901 attatctttt caggtaattc gagcagctga attagcaaaa gaaggtaaaa cagtagagaa
    12961 aatccttgaa gaagtcaaaa aagtacaaga caatacaaaa ttatacatcg gaatatccac
    13021 actagataac ttggtaaaag gtggaagaat cagccgaaca actggtttgc tgtctagtgt
    13081 tttgaacata cgcgtggtga tgaatttcgc ccattctgaa ttaatccctg taactaaggg
    13141 gcgaggaaag aaaacgttcg ataaatggtt taataatttg aaaaatgaac tgaaagaatt
    13201 gccaaatgtg cgacaaatcg gtatttcaca tgcggatgca aaagaattgg cgcaagaatt
    13261 caaagagggt ttgcaagaat tattcccgga catgcatatc cctgtactgc atacgaatcc
    13321 ggttatcgct acgcatactg gtaaagacgc gtttgcaatc atgtattatg tagattagtc
    13381 gaaaagagga taagccctca gcctgaaagg cttgcagctt attctctttt tctttataaa
    13441 attaggaaaa taattgtttt cttttttcat aaagactacc tttttgataa aatttttgat
    13501 aacattagaa ccaatcgaat acaagaggtg aaaaaatgaa attaacgaaa cgacatttga
    13561 tcctcgtttt gttcccatta ttaattgcga tcatcatcta tgcaggttta gctgcttcta
    13621 taccgaaagc tggcccacta atgaaaccag taactactgt gagagaagat ccaaataaaa
    13681 aagaaaaact tcactacata gctatcggag attcgctgac tgaaggagta ggagatcaaa
    13741 ccaagcaagg aggattcgtt ccccttgtcg caaatgatct aaaagaccgt tataatttga
    13801 ccgcggttga aacagaaaac tatggtgtaa atggtgaacg aagtgaccag atcttaaagc
    13861 gagtaaaaac aaaagaagaa attcgaaaca atatcaaaac agctgatttt atcacactga
    13921 ctgttggcgg taatgatttg atgaaagtta tccaaaataa tttattcggg ttaacggtca
    13981 attcctttaa aaagccactg aaaaactatc aggaaaatgt cacagaacta ttggaagaat
    14041 ttcgatccct caataaaacc gcacctattt acattttagg catctacaat ccattttatt
    14101 tgaactttcc tgagatcact gacatgcaga caattgttaa caattggaac aaaggaacag
    14161 aaacggtgac aaaaaaaatg ccgaactgct actttatccc aatcaatgat ttgctttatc
    14221 aaggattgaa tcaggaagta ggtgtcacag aagaaggaac agatcaaaca actagcacga
    14281 cagaaaatct aaatggcatc aaaaataatg tgctctatga agaagatcat ttccatccaa
    14341 ataatttagg ttatcaattg atggcaaatg cagtgagaga tcagctgatc gagacacaaa
    14401 accaatggtt agagaaggag gatgatcaat aaacatgaaa acaagatctg acgtatcttc
    14461 aaacaaaaca aagaaaacaa attactggaa attcgccttc cttatccttc ttggggtagt
    14521 acttggttcg accgtatttt taggcagccg tatttttgct aatcgggaac cagaactccc
    14581 agaaatacca gctcttacgg aaagacaagg agatcctgtc ttaacgatca attcaaataa
    14641 agaaaaagtc aatcaaatca tcagcttttt ccttagtgag tatcaaaaag atagtgatat
    14701 tgaatataaa ttttatttgg aaaatgaagc actactcaat ggtactttcg aagtactagg
    14761 ttttcctatc gatttctatt tatattttga tccgtatgtg atggaaaacg gaaatgtcca
    14821 actaaaagct agaagtcttt ctatcggaac attgagtttg ccaatcaaag atgtgatgaa
    14881 tatgatcaag agaaactata aattaccaga ttggatcgaa gtcaatacag atgatctgac
    14941 tatcatgctt aggttggatc aattccgaat gcaaaacggg atgtatatta aagcagataa
    15001 aatcgactta gtaaatgatg acattcggtt tagtctatat ttgccaaaag atacctctga
    15061 tgcttcaaac aactcaaata ccaacaaaac tagcaccagc agtactgaaa gcaagacaaa
    15121 agaaaataaa aaatcatcgg atgagtagta ttagaggggg aatcataatg gaacgagcaa
    15181 tatttgcagg aggctgcttc tggtgtatga tccagccttt cgacacgctt cctggtatcc
    15241 atacagtgat gtcaggatat actggagggc atgtaccaga tccgacttat gaacaagtaa
    15301 aaagccaaac gactggacat acagaagcag tggagattat ctatgatcca acattgattt
    15361 catatgaaca attagtagaa ctttactggc agcaaactga tccaactgac gctttcggac
    15421 aattcgagga tcgcggtgac aattatcgcc cggtgatctt ttatttgaca gaagaacagc
    15481 ggatcattgc tgaaaaaagt aagaaacagt tagctgaaag tggacgtttc gaacagccaa
    15541 tcgttacaaa aatcgaacca gcccaaccat tttatctagc agaagaagac catcaagagt
    15601 attataaaaa gcatcctgaa aattttaaaa gaaaccatgc acgccgggct gcctttatcg
    15661 aagaaaattg gaggaacgaa gggtgaatcg aagctttttt cattatttaa tgacactgcg
    15721 aggcggtggc acacatgatg ctttatctgt tttcgctaca gaagccggta aagacatcca
    15781 atttcctaag cacagctcta gctacgaaga aatttctaat tatctggaac tgaatgtcga
    15841 ctatttgccg tccatggata tctttgacga agcatgggaa aaatatttag aaaataatca
    15901 tgacatataa gagatagttt tgtactttat gatcaaaaaa gttttttgtc caatagccta
    15961 attgaaagaa ttatttttat ttcagctatc tagtcgcatt tttttgatat ttttcttata
    16021 ataaagaaag aatgaaaaaa agagggatgg attgatagag attgacaaaa aaacaacgct
    16081 atattaatcg cttctggctt gtattcaaat acttacttgt ttctgtattc cttgcgttca
    16141 tgttgcgggg atttttattc atcccggtac cagtggaagg caattcaatg gaaaacgtgt
    16201 tgaagcaagg tgatatggtt gtgatggaaa aattttcgga gatcagacga tttgatattg
    16261 ttgtttttca attagcagat ggcacgattt atatcaaacg agtaatcggt cttccaggag
    16321 aaaatgtcag ctaccaaaat gatcaattga agatcaatgg gaaagtagtt aaggaacctt
    16381 atctaactaa aaacctgaaa tcagatcatg caaatgcatc ctatacgacg gactttacac
    16441 tacaagagct aacgggacaa tccaagcttc cggaagataa ttattttgta cttggagata
    16501 atcgaagagt ttcaaaagat agtcgttcgt ttggcacgat caataaaact gatattctag
    16561 gtaaagcaag atttgtctat tatccacttg atgaaataaa atggatccag tgagaaacaa
    16621 taaagcgtgc agtctgttga gactgcacgc tttattgttt aatccaaaat cagtttacta
    16681 ccttagtacg taaaatcaaa cagctcgttt aaacactttt ctagcctcta aagttttgat
    16741 aaatgaataa acaaccatta tttgaatagg gatttggatc atattcctga tgaatctacc
    16801 tagaaaaatt actttgaaat ctgcaccaaa caaaacagac atcagccaag aattgataaa
    16861 aaggctgact gggatcataa tcaataattg ggcagcgatg acggaaggca ggtttatctt
    16921 tttatgatat aaaaataagc cattaataaa tccaccaagg aaagccgtga taagaaaagc
    16981 agcttgaaac gttcctgaac caaatatcgt gaaatttaaa gtgttcgaca acatttcaag
    17041 caaaccagcc cagatcggtc catatagata acccataagc gccatactta aaaatccgaa
    17101 tgtgatttga aaataagcag gaccaatcgc tatcttgtat aagatgacat caagggctaa
    17161 taaaagagca gaggtcgaca acaatcttgc attcatttcg ggcaatctaa aaacgtcttt
    17221 tgcaatcaaa gtgttcaaaa tattctctcc tttgtcagtt aataataaaa ggataccata
    17281 aaatcaccaa tgtgtcatga cacattggtg atttacatac actggcatct aacaattaaa
    17341 aaaagggtga gaagactgct tttataaacc gccgattgta aaattaagct agacaactaa
    17401 aaaagtcat
//
LOCUS       DACGLW010000206        12200 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.guided.089, whole genome shotgun sequence.
ACCESSION   DACGLW010000206 DACGLW010000000
VERSION     DACGLW010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12200
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.guided.089"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            14..238
                     /locus_tag="IXT53_003020"
     CDS             14..238
                     /locus_tag="IXT53_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002570387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906759.1"
                     /translation="MSGTYLSIYNWFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFL
                     IIAIIAVGLVFNAAGVKDILLELFNRIIGA"
     gene            355..852
                     /locus_tag="IXT53_003021"
     CDS             355..852
                     /locus_tag="IXT53_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HAQ4906760.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSISELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMSDVARYYIEETGALGEVPASLQNYIDYESYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            939..1331
                     /locus_tag="IXT53_003022"
     CDS             939..1331
                     /locus_tag="IXT53_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ4906761.1"
                     /translation="MKKIRSYTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAV
                     MILGNLPPLSMIEGAFLKYFGIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRRKLTY
                     AGKKVTLGRNQPQEAITAVRSEFYGISN"
     gene            1315..3762
                     /locus_tag="IXT53_003023"
     CDS             1315..3762
                     /locus_tag="IXT53_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ4906762.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSILSAQERSKNEVTGKLKTVAYDKIDQQTDALISM
                     IGENQVDYRFFIGFKLLLNDQEFSMKSLTVEAKNAFTDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYPLSKKKLEH
                     ETLIKTYDLIKPTRCLVEEKQRYLKIQQEDETAYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKAERGRWK
                     ETLPEISHEINIVNLTSDEKNKGLLDPYVIMKNTKDSESLAIDILTFLTGISSRDGER
                     FPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTDYDFAHLLFSDGY
                     VEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVISTFALDFIHTDRS
                     IFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDDLLDEKLKNNLGL
                     KFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLYGRVGVIQFHPVF
                     EELLHAFDTRPPVRKEV"
     gene            3765..5948
                     /locus_tag="IXT53_003024"
     CDS             3765..5948
                     /locus_tag="IXT53_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HAQ4906763.1"
                     /translation="MKPSILNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVTPSGLSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHALMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNMTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRM
                     MRKPRMLMHAHMHRLQRKLGRSVTALGAGSAIASATGKKGQSGSGSSASTQADHSRPN
                     GQEKSTLGKRIGQTIGTVADTKDRMVDTAGNLKEQVKDLPTNARYAVYQGKSKVTDNV
                     HDLTRSISQTKADKASGRKKQQEQRRKTIAERRSEMEQVKQKKQPASSVHERPATKQE
                     QSHDGQTSRQTTVQASYRESQQAKQERPTVKSDSSSLKTERQSNTVQERTVQKPVTST
                     APTDRASQRSITKERQSTVQRVPLQNTKSRPPIKTATIKKGSKKP"
     gene            5945..6946
                     /locus_tag="IXT53_003025"
     CDS             5945..6946
                     /locus_tag="IXT53_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase P60"
                     /protein_id="HAQ4906764.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDGGFSNIHYGGV
                     DVSAEVLAHKPMVEKVAKEYGIEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLGVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGDEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKFEGWKYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTRHIPLLEAKA
                     GDLVFFHSTYNAGSYITHIGIYLGDNRMFHAGDPIGYADLTSSYWQQHLVGAGRIKQ"
     gene            6961..7875
                     /locus_tag="IXT53_003026"
     CDS             6961..7875
                     /locus_tag="IXT53_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HAQ4906765.1"
                     /translation="MMKFGKNQNTEKQTPKEKKPRVYKVNPRKKVVIVLWVLLGLSFS
                     FAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWELNDKSIDNRIEN
                     LKAYLTEELQALNVDTVRKDIPVSSSVKGFQIWTVEADGDNQFDVTYSVDQLITEGEN
                     TKTVHSAYIVSVYVDSTGNMVLIKNPTITSIPKKSDYKPKAIESEGTVDSITTNEINE
                     FLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIYNRKDNQVTVSLTVEY
                     IDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            8120..8236
                     /locus_tag="IXT53_003027"
     CDS             8120..8236
                     /locus_tag="IXT53_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HAQ4906766.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            8252..10171
                     /gene="tet(M)"
                     /locus_tag="IXT53_003028"
     CDS             8252..10171
                     /gene="tet(M)"
                     /locus_tag="IXT53_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019117172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HAQ4906767.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            10269..11642
                     /gene="tet(L)"
                     /locus_tag="IXT53_003029"
     CDS             10269..11642
                     /gene="tet(L)"
                     /locus_tag="IXT53_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HAQ4906768.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIKKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
BASE COUNT         3818 a         2182 c         2563 g         3637 t
ORIGIN      
        1 tgagatattt ataatatcag gaacttacct atccatatat aactggtttc tatcaaacgc
       61 tcaatcactg gtgcttttag caatcgttgt gattggcttg tatcttggct tcaagcgtga
      121 gtttagcaaa ctgattggct ttttaattat tgcgattatt gcggttggct tagtcttcaa
      181 cgctgctgga gtaaaagaca ttttactaga gctattcaat cgcattattg gtgcttaaat
      241 aaaaccgttc ttttgtggaa tataagtggt tttcttatgt tccgcaaagg aatggtacac
      301 caaacgaagt gcggtaggga tttttgaatc tctacaaaga aaggacgtga atatatggac
      361 gatatgcaag tctatattgc gaatttaggc aaatacaatg agggcgaatt ggtcggtgcg
      421 tggtttacct ttcccattga ctttgaggaa gtcaaagaga aaatcggctt gaatgatgaa
      481 tatgaggaat acgccattca tgattacgag ttacccttta cggttgacga atacacttct
      541 attagcgaac tcaatcgcct atgggaaatg gtatcggagt tgccagaaga actacaatcg
      601 gagctatctg ctctgctcac tcatttttca agtattgaag aactaagcga acatcaagaa
      661 gatattatca ttcattccga ttgtgatgat atgtctgacg tggcacgcta ctacattgaa
      721 gaaacgggtg ctttaggcga agtgccagcc agtcttcaaa actatattga ttatgaatcc
      781 tatggtcggg atttagacct ttcgggaacg tttatctcaa ccaatcacgg gatttttgaa
      841 atcgtctatt aaatctgtcg gtacattctt actggcagat tttcttgttt acggggtagt
      901 caaaagacta tccccatttt tatgaaagga ttgattacat gaagaaaata cgaagctata
      961 ccagtatctg gtctgttgag aaagtgttgt actccatcaa tgactttaga cttccgtttc
     1021 ccataacctt tacgcaaatg acatggtttg tcgtgtcact ctttgcagtt atgatacttg
     1081 gcaacttgcc ccctctttct atgatagagg gagcatttct caaatacttt gggattcctg
     1141 tggctttcac atggtttatg tctacaaaaa cctttgatgg taaaaagcct tatggatttt
     1201 tgaagtctgt cattgcttat gcactgcgac gaaagctgac ctatgcagga aaaaaagtaa
     1261 cgcttggcag aaaccagcca caagaagcca ttacagcagt taggagtgaa ttttatggca
     1321 tatccaatta aatatattga aaacaatctg gtctggaata aagatgggga atgttacgct
     1381 tactatgagc ttgttcccta caattactca tttctaagtc cggaacagaa aatacaagta
     1441 catgattctt tcagacagct tatcgcacaa aatcgtgatg gcaagattca tgctttacaa
     1501 atcagtacag aatccagcat actttctgca caagaacgtt ccaaaaatga agtcactgga
     1561 aagctcaaaa cggttgccta tgacaaaatc gaccaacaga cagacgcttt aatatccatg
     1621 attggcgaaa atcaagtgga ctaccgtttc tttatcggtt ttaagttgct tctcaacgat
     1681 caggagtttt ctatgaaaag tcttaccgtt gaagcaaaaa atgcttttac tgattttgtc
     1741 tatgatgtga accataagct gatgggcgat tttgttagta tgagtaatga tgaaatcctg
     1801 cgttttcaga agatggaaaa gcttttagaa aataaaatct cccgtcgttt caaaatccga
     1861 agattagata aggatgactt cggctatctg attgaacacc tttacggaca gacaggcact
     1921 gcctatgaag agtatgagta tcctctatca aagaaaaagc tggaacatga aacactgatt
     1981 aaaacctatg acctcattaa gcctactcgc tgtctggtgg aagaaaaaca gcgatatttg
     2041 aaaatccagc aggaagacga aaccgcctat gtagcttact ttaccatcaa cagtattgtc
     2101 ggcgaactgg acttcccgtc ctctgaaatc ttctactacc agcaacagca atttaccttt
     2161 ccgattgata cgtcaatgaa tgtggaaatt gtagcgaatc gtaaagccct atctactgtc
     2221 cgcaataaaa agaaagaact gaaagacttg gataaccacg cttggcaaag tgataatgaa
     2281 accagctcta atgtggcgga agctctggaa agtgtgaacg agctggaaac caatttagac
     2341 caaagcaagg aatctatgta taagctgtcc tatgttgtaa gggtatcagc aaatgacctt
     2401 gacgaactta aacgtcgttg taatgaagtg aaagatttct atgatgattt gagcgtgaaa
     2461 ctggtacgac cttttgggga tatgctgggc ttacatgaag aatttttacc tgccagcaaa
     2521 agatatatga atgactatat tcaatacgtg acctctgatt tcctcgctgg tttaggtttt
     2581 ggtgctactc aaatgctggg tgaaaatgag gggatttatg ttggctacag cttagatact
     2641 ggacgcaatg tctatctgaa acctgctctt gccagtcaag gggttaaggg ttcagtaacc
     2701 aatgcgttag cgtctgcctt tgtcggttcg ctgggtggtg gtaaatcctt tgcgaataac
     2761 cttatcgtct attatgcagt gctttatggg gcacaagcag tgattgtaga cccaaaagca
     2821 gaacgtggca gatggaaaga aaccttgcca gagatttccc atgaaatcaa tatcgtcaat
     2881 ctgacttctg atgagaaaaa caaaggctta cttgaccctt atgtaattat gaaaaatacc
     2941 aaagattctg aatcactggc tattgatatt ttgacattcc ttacggggat ttcctctcgt
     3001 gatggggaac gcttcccaat ccttagaaaa gccattcgtg cagtaaccaa tagtgaagtg
     3061 cgagggttga tgaaagtgat tgaagaatta cgggttgaga atacgccact aagtaccagc
     3121 atagccgacc atatcgaaag ttttacagac tatgactttg cccatctgct ttttagtgat
     3181 ggttatgtag agcagtctat cagccttgaa aaacaactga acattataca ggttgccgac
     3241 ttggtacttc ctgacaagga aacttccttt gaggaatata ccacaatgga gttactatcg
     3301 gtagcaatgc tgattgtcat tagtaccttt gcgttggact ttatccatac agaccgaagt
     3361 attttcaaga ttgtagactt agacgaagca tggagcttct tacaggtggc acaagggaaa
     3421 acactatcta tgaagctggt tcgtgctggt cgtgctatga acgctggggt atatttcgtg
     3481 acccaaaata cagatgacct cttagatgaa aaactgaaaa ataacctcgg cttaaaattt
     3541 gcctttcgtt ccactgacct taacgagatt aaaaaaaccc ttgccttttt tggtgtagac
     3601 ccagaggacg aaaacaatca gaagcgattg cgtgatttgg aaaacgggca atgccttatc
     3661 agtgatttat atggtcgtgt cggtgtgata cagttccacc ctgtatttga agaactgctc
     3721 catgcctttg ataccagacc acctgtgcga aaagaggtgt aaatgtgaaa ccatcaatat
     3781 taaacagaat aaaatcaaac tggacgctga aacgtctagg taaagtggca atgacagtgg
     3841 ctttcacact tgtgattgcc atttttcttt tagccatgct gggaacggtg gttcaagctg
     3901 cgggcttggt agatgatacg gtcaatgtgg caaatgaata cagccgatac ccacttgaaa
     3961 actatcaact ggatttttat gtggataata gctggggctg gctaccgtgg aactggtcgg
     4021 acggaattgg aaaacaagtc atgtatggac tatatgccat taccaatttt atttggacaa
     4081 tcagtcttta tgtttccaat gcgacgggtt acttagtaca ggaagcctat tccttagact
     4141 tcatttccgc tacagcagat tccattggca agaatatgca gacccttgca ggggttacgc
     4201 ctagtggact atcaacagag ggtttctatg ttggattcct tttactcttg attttggttc
     4261 ttggggttta tgttgcctat acaggactga taaagagaga aaccacaaag gcaattcatg
     4321 ccctcatgaa ttttgtgctg gtttttatcc tatcggcttc ctttattgcc tacgctcccg
     4381 actacattaa aaaaatcaat gacttttcat cagacatcag caacgctagt ttgtcactgg
     4441 gtacaaaaat tgtcatgccc cattcagata gtcaaggcaa ggacagtgtg gacttgatac
     4501 gtgacagcct attttccata caggttcagc aaccgtggct actgcttcaa tataacagtt
     4561 cagacattga aagtattggt attgaccgtg tggaaagcct gctctcaacc agtccagatt
     4621 ccaacaatgg ggaagacaga gaaaaaattg ttgcggaaga aattgaagat agaagcaata
     4681 ccaatatgac gattacaaag acgattaacc gtttaggtac agtcttcttc ctatttgtct
     4741 tcaatattgg gatttccata tttgtattcc tattaacagg aatcatgatt ttctcgcagg
     4801 tactttttat catctatgct atgtttctgc ctgtgagctt tattttaagc atgattccat
     4861 catttgatgg tatgtcaaaa cgagccataa caaagctctt taataccatt ttgacacgag
     4921 ctggaatcac attgattatt acgacagcat ttagtatttc aaccatgctc tataccttat
     4981 cggctggtta tccgttcttt ttgattgctt ttctacagat tgtgaccttt gcaggaatct
     5041 actttaagct gggcgattta atgagtatgt tttctctaca gagtaacgat tctcaaagtg
     5101 tgggaagtcg tatgatgaga aaacctcgta tgcttatgca cgctcacatg caccgtctac
     5161 agcggaaact tggacgctcc gtgactgctt taggggctgg gtctgccatt gccagtgcta
     5221 cgggtaaaaa aggacagtcg ggttcgggaa gttctgcgag tacacaagca gatcactctc
     5281 gaccaaacgg gcaggaaaaa tcaacactag gaaagcgtat cggtcaaacc atcggtacag
     5341 tagctgatac caaagacaga atggtagata ctgctggtaa tttgaaagaa caggttaagg
     5401 atttgccgac caatgcaaga tatgcagtat atcaaggaaa atctaaagtg acggataatg
     5461 tccatgattt aaccagaagt atttctcaaa ccaaagcgga taaagccagt ggacgcaaga
     5521 aacagcagga acaaagacgg aaaaccattg cggagcgtcg ctctgaaatg gaacaggtca
     5581 aacagaaaaa acagccagct tcttccgttc atgaaagacc agccactaag caagaacaat
     5641 ctcatgatgg acagacttca aggcaaacaa ctgtacaggc ttcctatagg gaatctcaac
     5701 aagccaaaca agagcgtcca acagttaagt ccgattcttc aagtttaaaa acggaacgtc
     5761 aaagtaatac agttcaagaa agaacggttc aaaagccagt aacttcaact gcaccaacag
     5821 atagagcttc acaacgttca atcacaaaag aacgtcagtc tactgttcaa agagtaccac
     5881 tacaaaatac aaaaagtaga ccaccaatca aaaccgccac cattaagaaa ggcagtaaga
     5941 aaccatgaag ctgaaaactt tagtgattgg tggttcggga ttattcttga tggtcttctc
     6001 actgcttctg tttgtcgcca ttttattttc agatgaacaa gatggcggtt tttccaacat
     6061 tcactatggc ggtgtggatg tttccgcaga agtgctggct cataagccta tggtagaaaa
     6121 agttgccaaa gaatatggaa ttgaagaata cgtcaatata cttcttgcga ttatacaggt
     6181 ggaatcgggt ggtacagcag aagatgttat gcagtcctcg gaatccctcg gtcttccacc
     6241 taattcattg agtacagaag aatccattaa gcaaggtgtg aagtatttca gtgagttatt
     6301 agccagtagc gaaaggcttg gtgtagattt agaatcggtt atccagtcct acaattatgg
     6361 tggtggtttc ttaggatatg tggctaatcg tgggaataaa tatacctttg aactggctca
     6421 aagtttctca aaagagtatt cgggtgacga aaaagtgtct taccccaatc ccatagccat
     6481 acctatcaat gggggctggc gatacaatta cgggaatatg ttttatgtgc aactggtaac
     6541 gcagtatctt gtcacaacag agtttgatga tgatacagta caagccatca tggacgaagc
     6601 tctgaaattt gaaggctgga agtatgtcta tggcggagct tccccgacta cctcttttga
     6661 ttgtagcgga ctgacacaat ggacgtatgg aaaagctgga atcaacttac cacgaacagc
     6721 acaacagcaa tatgatgtaa cccggcatat cccattattg gaagcaaaag ccggcgattt
     6781 ggttttcttt cattctacct ataacgctgg ctcttatatt actcatatcg ggatttattt
     6841 aggcgataac cgtatgtttc atgcaggcga ccctatcggt tatgccgact taacaagctc
     6901 ttactggcaa cagcatttag tgggagcagg acgaatcaaa caataagaaa ggacgattca
     6961 atgatgaaat tcggaaaaaa tcagaataca gaaaaacaga caccaaagga aaagaaacct
     7021 cgtgtctata aggtcaatcc tcgtaaaaag gttgtgattg tcctatgggt acttttagga
     7081 cttagtttca gctttgcgat attcaagcac tttacagcca ttgataccca taccattcat
     7141 gaaaccacca tcatagaaaa ggaatatgtc gatactcatc atgtggaaaa ttttgtagag
     7201 aactttgcga aagtctacta ttcatgggaa ctaaacgaca agtccattga taatcgcata
     7261 gaaaacctga aagcatatct gacagaggaa cttcaagctc tgaatgtcga tacagttcgt
     7321 aaagatattc ctgtatcgtc ttctgtaaaa ggatttcaga tatggacggt agaagcagat
     7381 ggcgacaatc aatttgatgt aacctacagt gtagaccaac tcattacaga gggggaaaat
     7441 acaaagaccg tccactctgc ttatatagtg agtgtctatg tagacagtac tggaaatatg
     7501 gtcctcatta agaatccgac cattactagc ataccaaaga aatcagacta taaaccaaaa
     7561 gctattgaaa gtgagggtac ggttgattcc attacaacca atgaaatcaa tgagttttta
     7621 acgacgttct tcaagctcta tcctacagcg acagccagtg aactttccta ctatgtgaat
     7681 gacgggatat taaaaccaat cggaaaagag tacatctttc aagaactggt aaatcctatt
     7741 tacaatcgta aggataatca agtcacggta tcgctgacag tggagtatat cgaccagcag
     7801 accaaagcaa cgcaggtatc tcaatttgat ttggtacttg aaaagaacgg gagtaattgg
     7861 aagattatag aataacaaat attggtacat tattacagct attttgtaat cacgtactct
     7921 ctttgataaa aaattggaga ttcctttaca aatatgctct tacgtgctat tatttaagta
     7981 tctatttaaa aggagttaat aaatatgcgg caaggtattc ttaaataaac tgtcaatttg
     8041 atagtgggaa caaataattg gatgtccttt tttaggaggg cttagttttt tgcacccagt
     8101 ttaagaatac ctttatcatg tgattctaaa gtatccggag aatatctgta tgctttgtat
     8161 gcctatggtt atgcataaaa atcccagtga taagagtatt tatcactggg atttttatgc
     8221 ccttttgggc ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc
     8281 tcatgttgat gcgggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat
     8341 tacagaatta ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg
     8401 tcagagagga attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt
     8461 gaacatcata gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc
     8521 agttttagat ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg
     8581 tatattattt catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat
     8641 tgaccaaaat ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc
     8701 cgaaattgta atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac
     8761 cgaatctgaa caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat
     8821 gtccggtaaa tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa
     8881 ttgctccttg taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct
     8941 tatagaagtg ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg
     9001 cggaaatgtc ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct
     9061 ttatggcgga atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa
     9121 aattacagaa atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc
     9181 cggggaaatt gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac
     9241 aaagctattg ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt
     9301 tgaaccgagc aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga
     9361 cagtgacccg cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt
     9421 cttagggaaa gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga
     9481 gatagaacta aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata
     9541 taccattcac atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc
     9601 accgcttccg ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa
     9661 tcaatcattt caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata
     9721 tggttggaat gtgacggatt gtaaaatctg ttttaagtat ggcttatact atagccctgt
     9781 tagtacccca gcagattttc ggatgcttgc tcctattgta ttggaacaag tcttaaaaaa
     9841 agctggaaca gaattgttag agccatatct tagttttaaa atttatgcgc cacaggaata
     9901 tctttcacga gcatacaacg atgctcctaa atattgtgcg aacatcgtag acactcaatt
     9961 gaaaaataat gaggtcattc ttagtggaga aatccctgct cggtgtattc aagaatatcg
    10021 tagtgattta actttcttta caaatggacg tagtgtttgt ttaacagagt taaaagggta
    10081 ccatgttact accggtgaac ctgtttgcca gccccgtcgt ccaaatagtc ggatagataa
    10141 agtacgatat atgttcaata aaataactta gtgtatttta tgttgttata taaatatggt
    10201 ttcttgttaa ataagatgaa atatttttta ataaagattt gaattaaagt gtaaaggagg
    10261 aattaattat gaagtgtaat gaatgtaaca gggttaaata caaccaaatt ttaatttggc
    10321 tttgcgtttt atcttttttt agcgtattaa atgaaatggt tttgaacgtc tcattacctg
    10381 atattgcaaa tgattttaat aaaccacctg cgagtacaaa ctgggtgaac acagccttta
    10441 tgttaacctt ttccattgga acagctgtat atggaaagct atctgatcaa ttaggcatca
    10501 aaaggttact cctatttgga attataataa attgtttcgg gtcggtaatt gggtttgttg
    10561 gccattcttt cttttcctta cttattatgg ctcgttttat tcaaggggct ggtgcagctg
    10621 catttccagc actcgtaatg gttgtagttg cgcgctatat tccaaaggaa aataggggta
    10681 aagcatttgg tcttattgga tcgatagtag ccatgggaga aggagtcggt ccagcgattg
    10741 gtggaatgat agcccattat attcattggt cctatcttct actcattcct atgataacaa
    10801 ttatcactgt tccgtttctt atgaaattat taaagaaaga agtaaggata aaaggtcatt
    10861 ttgatatcaa aggaattata ctaatgtctg taggcattgt attttttatg ttgtttacaa
    10921 catcatatag catttctttt cttatcgtta gcgtgctgtc attcctgata tttgtaaaac
    10981 atatcaagaa agtaacagat ccttttgttg atcccggatt agggaaaaat atacctttta
    11041 tgattggagt tctttgtggg ggaattatat ttggaacagt agcagggttt gtctctatgg
    11101 ttccttatat gatgaaagat gttcaccagc taagtactgc cgaaatcgga agtgtaatta
    11161 ttttccctgg aacaatgagt gtcattattt tcggctacat tggtgggata cttgttgata
    11221 gaagaggtcc tttatacgtg ttaaacatcg gagttacatt tctttctgtt agctttttaa
    11281 ctgcttcctt tcttttagaa acaacatcat ggttcatgac aattataatc gtatttgttt
    11341 taggtgggct ttcgttcacc aaaacagtta tatcaacaat tgtttcaagt agcttgaaac
    11401 agcaggaagc tggtgctgga atgagtttgc ttaactttac cagcttttta tcagagggaa
    11461 caggtattgc aattgtaggt ggtttattat ccataccctt acttgatcaa aggttgttac
    11521 ctatggaagt tgatcagtca acttatctgt atagtaattt gttattactt ttttcaggaa
    11581 tcattgtcat tagttggctg gttaccttga atgtatataa acattctcaa agggatttct
    11641 aaatcgttaa gggatcaact ttgggagaga gttcaaaatt gatccttttt ttataacagg
    11701 aattcaaatc tttttgttcc attaaagggc gcgattgctg aataaaagat acgagagacc
    11761 tctcttgtat cttttttatt ttgagtggtt ttgtccgtta cactagaaaa ccgaaagaca
    11821 ataaaaattt tattcttgct gagtctggct ttcggtaagc tagacaaaac ggacaaaata
    11881 aaaattggca agggtttaaa ggtggagatt ttttgagtga tcttctcaaa aaatactacc
    11941 tgtcccttgc tgatttttaa acgagcacga gagcaaaacc cccctttgct gaggtggcag
    12001 agggcaggtt tttttgtttc ttttttctcg taaaaaaaag aaaggtctta aaggttttat
    12061 ggttttggtc ggcactgccg acagcctcgc agagcacaca ctttatgaat ataaagtata
    12121 gtgtgttata ctttacttgg aagtggttgc cggaaagagc gaaaatgcct cacatttgtg
    12181 ccacctaaaa aggagcgatt
//
LOCUS       DACGLW010000207        11192 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.guided.095, whole genome shotgun sequence.
ACCESSION   DACGLW010000207 DACGLW010000000
VERSION     DACGLW010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.guided.095"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            62..973
                     /locus_tag="IXT53_003030"
     CDS             62..973
                     /locus_tag="IXT53_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HAQ4906769.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            1126..1611
                     /gene="vanZ"
                     /locus_tag="IXT53_003031"
     CDS             1126..1611
                     /gene="vanZ"
                     /locus_tag="IXT53_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HAQ4906770.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(1898..2227)
                     /locus_tag="IXT53_003032"
     CDS             complement(1898..2227)
                     /locus_tag="IXT53_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ4906771.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            2532..2861
                     /locus_tag="IXT53_003033"
     CDS             2532..2861
                     /locus_tag="IXT53_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007723369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HAQ4906772.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            2851..3138
                     /locus_tag="IXT53_003034"
     CDS             2851..3138
                     /locus_tag="IXT53_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HAQ4906773.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPILLCGTHENFYEQLKKIR"
     gene            complement(3417..3980)
                     /locus_tag="IXT53_003035"
     CDS             complement(3417..3980)
                     /locus_tag="IXT53_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ4906774.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAV
                     RPQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQ
                     VYSVKEITEMTGISKATLYRYLKDNRE"
     gene            complement(4173..4745)
                     /locus_tag="IXT53_003036"
     CDS             complement(4173..4745)
                     /locus_tag="IXT53_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HAQ4906775.1"
                     /translation="MNKAIFWDLLGTLGGDSRTLINQNFNFFKDTIPALKKAAQSHYL
                     NIIITNQSHISHGRITMAEYTDALESLINELEKNDIQITDVYTCPHARKDNCDCKKPQ
                     PTFVNEALKKYDLNPSKCFVVGDSGKNDMMLAKNAGINSVLLLTGEGIDSLTKYRHLW
                     SETKPTFIAENSLDAINKIHMYADGDSSDS"
     gene            5533..6108
                     /locus_tag="IXT53_003037"
     CDS             5533..6108
                     /locus_tag="IXT53_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906776.1"
                     /translation="MKNKFKYLFTILALLPITTSTLITPTIAKASEKQEDSVSYEIVK
                     NDLYIQFEEELAELIASNQSTVSVPDLFSTQSVLSTSSQDNSISEEQFFSLVEKYDGA
                     ELTAVVNSQDAFTTKFVNGGVNSYATSYRGLNALRSELKYNAGALKVGGTLVGTAGGL
                     LGVLLGMVTGDYLSGHCDDAAAGVSAFIQAG"
     gene            complement(6291..7043)
                     /locus_tag="IXT53_003038"
     CDS             complement(6291..7043)
                     /locus_tag="IXT53_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HAQ4906777.1"
                     /translation="MMLNEQTEVKLAELKLSGMLESYREQMTNKEYQSMSFEDRFNLM
                     VDLEHSRRKNNKLQRLIKSANFRNASASIEDIEYHPDRNLDKNLILKLASGTYLEEHH
                     NIILMGASGNGKTYIANAFGIQACRQYYNVKYIRLPELLDELTISKNQADGSFRKIIN
                     RYKKVQLLIIDEWLLTNLSLENSTFLLEIIESRLKTASTIFCSQFHPEGWHEKLGNPQ
                     IADAILDRIIHDSYQILVDGKVSMRERYGIRN"
     gene            complement(7043..8584)
                     /locus_tag="IXT53_003039"
     CDS             complement(7043..8584)
                     /locus_tag="IXT53_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010632830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HAQ4906778.1"
                     /translation="MFRYRRILEMHSEGISIRSIAASTGNSRQKITEVIRKAEEKGIA
                     RALSEEMTDQWLGEFLFPEKQLEANGRHLPDFDKIHKELAKPNVTLTLLHQEYAVECR
                     MSGKIPYAYRTFCDYYNAFARKHKATMRIRRKPGEIMEVDWAGSPAYVIDRDTGEKVK
                     AYVFVATLPCSQLSYAEAFLTMKSVAWITAHNHAFEYFGGSTDIIVPDNLKTGVKKNE
                     GIDVILNESYRELGEHYGSIILPARVSTPKDKPSVEGSVGVISTWIIAALRNTHCFTI
                     EDLNREIRMKLNEFNERPFTKKPGSRIRAFEEEEKFALNPLPLAPYKMSVWKKAKVQL
                     NYHVSVESMFYSVPYEYIQQNIDIRMTENLIELYFNSMRIATHKRLYGKFGQYSTVRD
                     HMPDNHKMYVDHTPESSMEWAETIGSSTVKVVQHLLDRYQNEKQALKSIMSLKSFGKK
                     YSKNEIETACSTALNLASLPSVKIIQTILKGNKQKRATSQSHIEDTNIEKHGFTRGAS
                     YFGGK"
     gene            9498..9962
                     /locus_tag="IXT53_003040"
     CDS             9498..9962
                     /locus_tag="IXT53_003040"
                     /inference="COORDINATES: protein motif:HMM:NF037267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906779.1"
                     /translation="MRQVLLVVTLCLSFFLFEVNTVEAYVANNGVYYNLIQGGTQEFM
                     EVDEEGNPILIRVEEVPGLSLFSVNNGNYKISGSKFGMWKANYYVTVSNNQFIRAYSP
                     SAVAVTGSFTSTRLSLVSSKKASYTLEWKAGILSSNSYLDATLTDNSINITY"
     gene            complement(9959..10483)
                     /locus_tag="IXT53_003041"
     CDS             complement(9959..10483)
                     /locus_tag="IXT53_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007722706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HAQ4906780.1"
                     /translation="MQNDIRGKIKKSRHIKGISQSEVAKYLNISRQSISKWENDTSSP
                     DINNLTLLSSLYEISIDDLLKEKKEVKKNIKENSIEIIESKNKLIYLKNNTNRDEGLV
                     LLLISLIGCLISPLGLIIAPVVLKRNKATNTFYKGVIAASVICFTVSLFVSYGHFSNI
                     FHWGGSTNVEYIEN"
     gene            complement(10533..>11192)
                     /locus_tag="IXT53_003042"
     CDS             complement(10533..>11192)
                     /locus_tag="IXT53_003042"
                     /inference="COORDINATES: protein motif:HMM:NF015755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HAQ4906781.1"
                     /translation="LFDSRFKTLQQNRYVDIIGYDTYDKINHAYAFGGVAMTINTIQA
                     YLDIPIDFFVTLNMQGLEQIVDSIGGITLTPTATFTQDSYSFYQGEIINLDGKASLAY
                     SRNRHADDSGDYGRQARQREIIMAIIEKGTKVTTLATKIPSLIKSLQGNMTTNLSVDD
                     MWTIINKYSTALGNIEQVQLKGEGQLLDDGIYYEMINEESLEEVQNILQKQLDYHLNI
                     D"
BASE COUNT         3569 a         1862 c         1956 g         3805 t
ORIGIN      
        1 aggaggacaa tttatttaca caaaattatt gacgctccca tagttaagga ggagactgaa
       61 aatgaagaag ttgttttttt tattgttatt gttattctta atatacttag gttatgacta
      121 cgttaatgaa gcactgtttt ctcaggaaaa agtcgaattt caaaattatg atcaaaatcc
      181 caaagaacat ttagaaaata gtgggacttc tgaaaatacc caagagaaaa caattacaga
      241 agaacaggtt tatcaaggaa atctgctatt aatcaatagt aaatatcctg ttcgccaaga
      301 aagtgtgaag tcagatatcg tgaatttatc taaacatgac gaattaataa atggatacgg
      361 gttgcttgat agtaatattt atatgtcaaa agaaatagca caaaaatttt cagagatggt
      421 caatgatgct gtaaagggtg gcgttagtca ttttattatt aatagtggct atcgagactt
      481 tgatgagcaa agtgtgcttt accaagaaat gggggctgag tatgccttac cagcaggtta
      541 tagtgagcat aattcaggtt tatcactaga tgtaggatca agcttgacga aaatggaacg
      601 agcccctgaa ggaaagtgga tagaagaaaa tgcttggaaa tacgggttca ttttacgtta
      661 tccagaggac aaaacagagt taacaggaat tcaatatgaa ccatggcata ttcgctatgt
      721 tggtttacca catagtgcga ttatgaaaga aaagaatttc gttctcgagg aatatatgga
      781 ttacctaaaa gaagaaaaaa ccatttctgt tagtgtaaat ggggaaaaat atgagatctt
      841 ttattatcct gttactaaaa ataccaccat tcatgtgccg actaatcttc gttatgagat
      901 atcaggaaac aatatagacg gtgtaattgt gacagtgttt cccggatcaa cacatactaa
      961 ttcaaggagg taaggatggc ggaatgaaac caacgaaatt aatgaacagc attattgtac
     1021 tagcactttt ggggtaacgt tagcttttta atttaaaacc cacgttaact aggacattgc
     1081 tatactaatg atacaactta aacaaaagaa ttagaggaaa ttatattggg aaaaatatta
     1141 tctagaggat tgctagcttt atatttagtg acactaatct ggttagtgtt attcaaatta
     1201 caatacaata ttttatcagt atttaattat catcaaagaa gtcttaactt gactccattt
     1261 actgctactg ggaatttcag agagatgata gataatgtta taatctttat tccatttggc
     1321 ttgcttttga atgtcaattt taaagaaatc ggatttttac ctaagtttgc ttttgtactg
     1381 gttttaagtc ttacttttga aataattcaa tttatcttcg ctattggagc gacagacata
     1441 acagatgtaa ttacaaatac tgttggaggc tttcttggac tgaaattata tggtttaagc
     1501 aataagcata tgaatcaaaa aaaattagac agagttatta tttttgtagg tatacttttg
     1561 ctcgtattat tgctcgttta ccgtacccat ttaagaataa attacgtgta agatgtctaa
     1621 atcaagcaat ctgatctttc atacacataa agatattgaa tgaattggat tagatggaaa
     1681 acgggatgtg gggaaactcg cccgtaggtg tgaagtgagg ggaaaaccgg tgataaagta
     1741 aaaagcttac ctaacactat agtaacaaag aaagcccaat tatcaatttt agtgctgagg
     1801 aattggtctc tttaataaat ttccttaacg ttgtaaatcc gcattttcct gacggtaccc
     1861 ccaaactaag atttcataga tagattttct gataacttta aaaaacacga atagcggaaa
     1921 ggatgtcttc attcgctaaa gagtaataaa ctacttttcc atcttgacgt gattttgcca
     1981 ttccatgttt ttttaaaaaa cgtaaatggt gtgacgttgt agcgacacta gcagtaagta
     2041 tttctgctaa atcacagacg cacatttcct tataggtttc taaagcatag aaaattttaa
     2101 ttctagaaaa atcgctgaaa catttcccta acacaagtaa gtttgaaaaa tctttttgat
     2161 ccaatttatt ttttattcca tttacttttt ctttatgtac gacagtcact tggcaaactt
     2221 ttttcattca tctcacctca cattcaaacg attatttgaa tgaagtatac ctatttgatc
     2281 actcttttgt caatctacct taattccaat acactattat aagcaatttc atgtattgaa
     2341 gtaaacttac ttgcaagtgc gtttttgatt ccaagttaaa aacatattat tattctacat
     2401 aatttcctaa tcaaataaaa ctcctttgaa aattgttcta aaggagtttt attattttgt
     2461 atttctacta aaaatagcgt acaatgtaat tactaagtaa ttacgaaatg taaaataatg
     2521 gaggaaataa tatgattaca aaaacaagaa aacaaggaaa ttctattatg ttaacggtcc
     2581 ctaaagaatt cgatgtgccc aatggtgttg aagtagaagc taagcttgta gagaatggta
     2641 tcctttatga gtttgtagag cctaaaaaag aattttttga ttttagtgaa gatattttgg
     2701 ctgatattct ttcagaaggt tatagcaaac aagagatctt aaaagaattt agaaatagaa
     2761 aaaatgcgtt aacttctgca tttaaatcta ttgctgaaga tactgttgca aattctaaac
     2821 cgatgacaaa agaggagttg gcagcagaaa ttggcttatg agattctacc ttctaaacac
     2881 gtaataaagt atctaaaaaa attaaaagag aaaacattaa aagaacaatt tttaacgata
     2941 atttatgatg agatagcagt tcgtcctcat tcgggagaac aaaaaacggg tgatttatca
     3001 ggaatttggg ctattggatt caagtatgca ggtacgacat atcgcgttgc atatgaaatt
     3061 aaagataatg cagtgatacc tatcttacta tgtggaacgc atgaaaattt ttatgaacaa
     3121 ttaaaaaaga ttaggtaaga acccaatgtt tcaacttgag aggatagacg acactttaaa
     3181 agataatttt taacagctga gagttgtaat accctataat tttaaaccac taacctatat
     3241 atttatgaaa aatagaaaaa attaaaacgc attttaagcc tctttcaaat taaaagcttc
     3301 atccttatgc acaaagaggg cttaaaatgc gttattttat attgtttttt agtaataata
     3361 aaaaaatcac tctaattctc ccataaatat tatagattcc taaagattaa aatagattat
     3421 tccctattgt cttttaagta gcggtagagt gttgccttgc taataccagt catttccgta
     3481 atttctttta cgctataaac ctgactttta tacaatttta ctgctcgatc aacatctttc
     3541 tggttcactc gtggacggcc acctttacga cctctcgcac gtgcactttt caaaccttct
     3601 ttggtacgtt caacaattaa atcacgttcg aactgactaa atgcttggaa gacagtcatc
     3661 attaagcgtc cgtgaggcgt gctggtatcg aagttttctt tcaagctcac cagcttaaca
     3721 tggtgttctt caaaataggt aacaaggtca atcaaatcct ttgtactacg acccaatcga
     3781 gaaaaacttt ctacaattac cgtatctccc ggacgaattt tatcttttaa acgatttagt
     3841 tggggacgaa ccgctttggt tcccgtcatt ttctcagtta ggatttcatt acaattttgt
     3901 tcattgagta agtctaattg acgatttagt tcttgtgaac gtgtactcac tcgggcataa
     3961 ccataaatgt atccactcat gcttttcttc ctttcgtact ttaattctgg cttgattgta
     4021 tcataaaagg gagttataga tacatagtta atgcaacaag ttatgataca aaatatgacc
     4081 tcttttattt aataaaagag gtcatttcat tttgtctcat aatcgattgt ttttgatacg
     4141 gtttttagta ataaatggag actcttctgg tatcatgaat cagaagagtc tccatctgca
     4201 tacatatgta ttttatttat agcatctaaa ctgttttcag caataaaagt aggttttgtt
     4261 tcactccaca gatgtctata tttggttaaa ctatcaattc cctctcccgt taaaagtaaa
     4321 acactgttta taccagcatt tttggccaac atcatatcgt ttttaccgga atcgcctact
     4381 acgaaacact tacttggatt taaatcgtat ttttttaatg cttcattgac aaatgtaggt
     4441 tgaggcttct tgcaatcaca attgtctttt ctagcatgag ggcaagtgta tacatccgtt
     4501 atttgaatgt catttttctc taactcattt attaagctct ctaaggcatc agtatattct
     4561 gccatagtaa tacgtccatg tgaaatatga ctttgattcg taatgatgat atttagataa
     4621 tgactttgtg ccgctttttt taaggccggt attgtgtctt taaaaaaatt gaaattttga
     4681 ttaatcagtg ttctactatc ccctcccagc gtacctaaca gatcccaaaa tatagcttta
     4741 ttcatggata tttgtctccc tctcatttta agaatagtta actaacaagc caaggtgctt
     4801 acagtattta aaagtagttc ttcgtaatta aaacaaaaat attacttttt gaaaaagcat
     4861 attttatttt gccagtttaa ctatatcata tttacaggag ccactctcta ttcactttct
     4921 atatgaactt cttttgaggg aatggatatt tcgaatcaca taatcactga tatagaagag
     4981 acttagaatt tcagttatat tttgatacag caaagagtgt aaaaaagaag ataccctcgt
     5041 tggcgaaaaa catttgaata caaaccttac ttttttgatg ggatttccat gctaggagca
     5101 cataaaaaac taaggtgata ataaatttat agtgatttag tgtggaggtt cttttgttaa
     5161 ttgaaaccat aaatagtaaa aaaatctatt tttaatgatt gtacatatta aagagaaaat
     5221 tgatatacac cagcttatca aagttgagat taatactact actttgacct atgaagatga
     5281 ttttataaat accttatagt aaagaaaagt tcaatttttt ctgctatcat ctttttttat
     5341 aaaagcaacg attagtttcc cagatgtaaa tagaaaagtg cttttgaaac agctcgaaaa
     5401 acataataat agattgtgag aggaggaaaa actattgcaa aatcattacg taagagatgt
     5461 tactcttgct tagatttttt atttttcaag ttgtggtatt aaaagtagaa aaaatagaaa
     5521 gaaggataaa aaatgaaaaa taaatttaag tatttattta ctattttagc cttattgcca
     5581 attactacat cgaccttaat tactcctaca atagccaaag cttctgaaaa acaggaagat
     5641 tctgttagtt atgaaattgt aaaaaatgat ctttacattc agtttgaaga agagttagct
     5701 gaattgattg cttccaatca atcaactgtt tcagtccctg atttattttc tacacaatca
     5761 gtattatcta cttctagtca agataattct attagtgaag agcaattttt ttctttagtc
     5821 gaaaaatatg atggagctga attgacagct gttgttaatt cacaagatgc tttcacaact
     5881 aaatttgtga atggtggggt taactcatat gctacttctt acagaggatt aaatgcattg
     5941 cgtagcgagt tgaagtataa tgctggtgct ttgaaagtag gaggtacctt agttggcaca
     6001 gctgggggat tgttaggagt attattaggc atggttacag gggattattt atctggccat
     6061 tgcgatgatg cagcagcagg tgttagtgcc tttattcaag ctggttgaat acggcatcga
     6121 aaatgtacca ctagagcatt gttttttacc actaagtgca aagaaatgta ccagtaagtg
     6181 cgacgcggtt accatttcaa ataataaaag cgacaataat gaccacaagg tcaactaaga
     6241 gccactcatg aaaatcatgg agtggctctt aatttatttt aatgactcat ttaattacgg
     6301 atgccgtaac gttcacgcat agaaacttta ccgtctacca gaatttgata agaatcatgt
     6361 atgatcctat ctagaatggc atcagcgatt tgggggttgc cgagcttttc gtgccatcct
     6421 tctggatgga attgagaaca gaaaatagtt gatgctgttt taagacggga ttcgattatt
     6481 tccaatagaa aggtggaatt ttctaacgaa agattggtta atagccattc atcgatgatg
     6541 agtaattgaa cttttttata tctgttgatt atttttcgaa aactaccatc tgcttgattt
     6601 ttcgagatgg ttaattcatc tagtagttca gggagtcgga tgtatttcac gttgtagtat
     6661 tgccggcatg cctgtattcc gaaggcattg gcgatatacg ttttcccatt ccctgaagcg
     6721 cccatcaaaa tgatgttatg gtgttcttcc aaataagtcc cactggctag ttttaaaatg
     6781 aggttcttat ctaaattacg gtccggatgg tattcgatat cctcaatgct ggcagaggca
     6841 tttctaaagt ttgcagattt aatcaaccgc tgtagtttat tattttttcg ccgggaatgc
     6901 tccagatcaa ccatgaggtt gaagcggtct tcgaaactca tggattggta ctctttattt
     6961 gtcatctgtt cacgatagga ttcaagcatg cctgaaagtt tcaattcagc taattttact
     7021 tctgtttgct catttaacat cattatttcc ctccaaaata ggacgcaccg cgagtgaatc
     7081 catgcttttc aatgttagtg tcttcaatat gtgattggct tgttgctcgc ttctgcttgt
     7141 tgccctttag aatggtctgg ataattttaa cagaaggcaa agaagccaga tttaaagctg
     7201 ttgaacaggc cgtttctatt tcatttttag aatacttttt accaaagctc ttcaaagaca
     7261 tgatcgactt taaggcttgt ttttcatttt gataacggtc taagaggtgt tgaacaacct
     7321 ttactgttga ggaaccgatg gtttcagccc attccatcga ggattctggg gtatggtcca
     7381 catacatttt gtgattatct ggcatatgat ctctgacagt cgaatactgc ccaaatttcc
     7441 cgtacagacg tttatgtgtt gcgattcgca tggaattaaa atatagttca attagattct
     7501 ctgtcattcg aatatctata ttttgttgaa tgtattcgta tggaacggaa taaaacatgc
     7561 tttcaactga gacgtggtag tttagttgaa cctttgcttt tttccagaca gacatcttat
     7621 aaggtgccag tggaagagga ttaagtgcga atttttcttc ttcctcgaag gctctgatcc
     7681 tggatcctgg tttcttagtg aacggtcgct cattgaattc atttaatttc attctgattt
     7741 cacgatttaa atcttcgatt gtaaaacaat gcgtattgcg taaggcagct ataatccaag
     7801 tggagatgac gcctacgctg ccttcaacac tgggcttatc cttgggagta gaaactcggg
     7861 caggtaaaat gatggacccg taatgttccc ctagctctcg atatgattca ttaaggatga
     7921 catcgatgcc ttcattcttt tttacacctg tttttagatt atcgggaaca attatgtcgg
     7981 tagaaccgcc aaaatattca aatgcatggt tatgtgccgt aatccaagcc accgatttca
     8041 tcgttaggaa tgcttccgca taagacaatt ggctgcacgg taaggttgcc acaaagacat
     8101 aagcctttac tttttcaccc gtatctcgat cgatgacata agctggcgat ccagcccaat
     8161 ccacttccat aatttcacca ggcttccgac ggatgcgcat agtggcttta tgctttcgcg
     8221 cgaacgcatt gtaatagtca caaaatgtac gataggcgta agggattttc ccactcatgc
     8281 gacactcaac tgcgtattcc tgatgaagaa gcgtcaaggt cacgtttggt ttggccaatt
     8341 ccttgtgaat cttgtcgaag tcaggcaggt gccgtccatt ggcttctaat tgtttttctg
     8401 gaaacaaaaa ttcgcctagc cattgatcgg tcatttcttc cgacaacgcc ctcgctatcc
     8461 ctttctcctc agcttttcgg ataacctcag taattttctg acgtgagttc cccgtgcttg
     8521 ctgcaatact gcgaatacta attccttcac tgtgcatttc aagtatcctt cgataacgaa
     8581 acataataaa aatacctcct gtaaaataaa tgtcacaccc taaggtgtgc acctacatta
     8641 tacaagaggt atttgtggta cactgctttg cacttactgg tacatttctt tgccgagcct
     8701 ggtaaaaatt attgcagaga tggtacattt tcgatgccgt aatcactggt tacactagag
     8761 gaggatctag ggtaaccctt actgaaggtt tccctattag ccatattgat agtatttatg
     8821 aagctgtaat taattaagca agaaatgtgg agatgataaa aagcagttct ctcttgggta
     8881 aagagaaaac tgcttttatc tttttcgcta atgctttttg gaattacctc gctgtctaaa
     8941 aagggaagtc acagaaaaaa gaattaacac actacctatt attattggta gtacagttaa
     9001 aagagtaatc ttactaagat caaaatcaga acttgctaca ctattaataa caaaaaacaa
     9061 gccaaccaaa aaagtaaata cagatagata tttattagtc ataaaagaat cttcctctcg
     9121 tatttttttg ttctaggaag ctatgctaat ctaagttctt cctaagtata gtgtctcatc
     9181 taagtatagc ttaattaagc atcttatggt ttacatcagg tcactttata gttgcaaccg
     9241 ctcaaatttg ctttttgatt ttattatcta cgttattagt aaaaaattag ttgatttggt
     9301 ttttttattt cgttgatatt ttatctgttt agtaagtaga gttggtaaca gtgaatgttt
     9361 actaacccag atacacttat atatgcgtcc atttgtagag agagccgttt caatataaga
     9421 atgttcacaa aatgttctct tactaattaa acccgtatat attaaactat gagtatatta
     9481 attttaggag ggattttttg agacaagttt tattggttgt tactttatgt ttatctttct
     9541 ttttatttga agtaaacact gtagaagcat atgtggcaaa taatggagtc tactataact
     9601 taatccaagg tggcacgcaa gagttcatgg aggtagacga agaaggaaat cctatcttga
     9661 ttcgtgtgga agaggttccc gggctctccc tgttctccgt caacaacgga aattataaaa
     9721 tatctggatc taaatttgga atgtggaagg caaattacta tgtgacagtc tcgaataacc
     9781 aatttattag ggcctactcc ccttctgcag tcgcagtgac cggatccttt actagcacga
     9841 gactgtctct cgtctccagc aaaaaagcta gctacacatt agaatggaaa gctggaattt
     9901 tatctagtaa ctcttatcta gatgctacac tgacagataa ttccattaat attacgtatt
     9961 agttctcaat atattccacg ttagtggagc cgccccaatg aaagatatta ctaaaatgtc
    10021 cataggagac aaacaggctt actgtaaaac agatgactga agcagcaatc actcctttat
    10081 aaaaagtgtt tgttgcttta tttcttttca gaacaacagg cgcaataatt agtccaagag
    10141 gagaaatcaa acagccgatt aaggaaatga gcaataaaac taacccttca tctcggtttg
    10201 tattattttt taaataaatt aatttatttt tagattctat tatctctata ctattctctt
    10261 ttatattttt tttaacttct tttttttctt ttaaaagatc atcgatagag atttcatata
    10321 aactacttaa taaagttaag ttgtttatat ctggggaact agtatcattt tcccatttag
    10381 aaatagattg tctagatata tttaaatatt ttgctacttc actttgagag atgcctttta
    10441 tatgccgtga ttttttaatt tttcccctaa tgtcattctg tatcctctcc tattaatgac
    10501 ttttatttat tggatattaa gaaatagatt atttagtcta tattcaagtg ataatctagt
    10561 tgtttttgaa ggatattctg aacttcttct aaagattctt cgttaatcat ttcatagtat
    10621 attccgtcat caagtagttg tccttccccc tttaattgaa cttgctctat atttcctaaa
    10681 gcagtggaat atttattaat aatagtccac atatcgtcaa ctgacagatt agtagtcata
    10741 tttccctgaa gactcttaat gagtgaaggt atcttagttg ctagtgtagt aactttagtt
    10801 cctttctcta taattgccat tattatttcc ctttgacgag cttgtctccc atagtcacct
    10861 gaatcatctg catgacgatt tcgagagtac gctaaggaag ccttcccatc taagttaata
    10921 atttcgcctt gataaaatga ataactatct tgtgtaaaag tggcagttgg agtaagagtt
    10981 attcctccaa tagaatcaac aatttgttct agaccctgca tatttaacgt tacaaaaaag
    11041 tcaataggta tatctaagta tgcttgtatt gtatttatag tcatggctac tccaccaaag
    11101 gcataggcat ggttaatttt gtcgtaagta tcataaccga taatatcgac ataacggttc
    11161 tgttgcaaag ttttaaatct actatcaaat aa
//
LOCUS       DACGLW010000208         7959 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.guided.111, whole genome shotgun sequence.
ACCESSION   DACGLW010000208 DACGLW010000000
VERSION     DACGLW010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.guided.111"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            128..1564
                     /locus_tag="IXT53_003043"
     CDS             128..1564
                     /locus_tag="IXT53_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HAQ4906782.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            1753..4200
                     /locus_tag="IXT53_003044"
     CDS             1753..4200
                     /locus_tag="IXT53_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HAQ4906783.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            4430..5497
                     /gene="rlmN"
                     /locus_tag="IXT53_003045"
     CDS             4430..5497
                     /gene="rlmN"
                     /locus_tag="IXT53_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HAQ4906784.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(5690..6793)
                     /locus_tag="IXT53_003046"
     CDS             complement(5690..6793)
                     /locus_tag="IXT53_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HAQ4906785.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            7346..7894
                     /gene="aac(6')"
                     /locus_tag="IXT53_003047"
     CDS             7346..7894
                     /gene="aac(6')"
                     /locus_tag="IXT53_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HAQ4906786.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         2664 a         1359 c         1729 g         2207 t
ORIGIN      
        1 tttcttacta ctcgaaacta agcgactttt tcacaacctc ctttacatat ttttattttg
       61 ttgtggaagg ggtttcgtgt tattctaaaa gaggaacgat tgaaggttat aggaaaggaa
      121 gagatgaatg gaacacaaag aacttacctc atttccaaaa gattttctgt ggggctctgc
      181 ttctgcagcg tatcaagtag aaggagcttg gcaagaagat gggaaaggag aatcagtctg
      241 ggatcgattt gtacggatac ctggaaagac atttaaagga acgaacggtg atttggcagt
      301 agaccactat catcgttata aagaagatat tgctttaatg aaagaacaag gtctgaaagc
      361 ttaccgcttc tctgtagctt ggacacggat ttttcctaat ggacgaggag aagtcaatca
      421 agctgggctc gctttttatg agagattgat cgacgaattg attgaaaacg atattgaacc
      481 tatgttgact ttatatcatt gggatcttcc acaagcattg caagatgaat ataatggttg
      541 ggaatcacgt cagatcattg aagatttcac tcattatgca gaaacattat ttgaggcatt
      601 ccgcgggaaa gtccattact ggattagttt gaatgaacaa aatatcttca cttcattagg
      661 ttatttgtta gcagcacatc cgccaggtgt gacagacccg aaacgaatgt atgaagttaa
      721 ccatattgct aatttagcaa atgcatctgt catcaataaa tttcacgagt tagaaatgcc
      781 tggaaaaatc ggaccaagct ttgcgtatac gccaaattat ccaattgaca gtaatcctga
      841 aaatgttctg gctgctgaaa atgcagaaga cctgatggcc aattattgga tggatgtcta
      901 tatgtgggga aaatatccga ttgctgccat gaggttctta gaagaaaaag gatgggcacc
      961 aacgattgaa gctggcgatg cagaattgct agaatctgct aaaccagatt ttcttgggat
     1021 caattactat caaacggcga cgaatgcatt caatccatta aacggagttg gtgctggaaa
     1081 aatgaatacg acaggaaaaa aaggttcatc agaagaaact ggagttcctg gaatgtataa
     1141 aaaagttgag aatccatttg tcgaacgaac aaattgggac tgggagattg acccagaagg
     1201 attaaggatc ggactgcgtc gtatcacaag ccgttaccgc atgccggtac tgattacgga
     1261 aaatggctta ggagagtatg acaagttgac agaagataaa caaatccatg acgactatcg
     1321 aatcaattac ttacaaagcc acgtcaaagc catcaaagaa gcaatttcgg atggagcaga
     1381 agtcctaggc tattgcacct ggtcttatac agatttgctt agctggctga acggttatca
     1441 aaagcgatat ggatttgtat atgttgatca agacgaaaca caagaaggat ctttggaacg
     1501 atataagaaa gacagttatt actggtacca aaaactgatt gaagaaaatg gagagaacct
     1561 ttagttcaca tacttaaaac aagaaggtac ggctggatat ggccgtgcct tttcttgata
     1621 aaatcattta gaaattaaga aaaactgtaa aataagctga aaaatgaaag aaagcgtcag
     1681 ttctttcaaa caaaacttta gttcccttac ttttttttgt ataatgaaac ttgatgaaat
     1741 gagggataat aaatggcaca aaagaaacgg cctactaaaa aaaggaaaaa gacgaaaaaa
     1801 acgaaaaagc aaaaacagca gcaggaacaa atgctgacga ttggtgttgg gtttgccttg
     1861 attttatttg ctgtcttcgg ctttttaaaa ttaggttttt taggaatatt gattgcaaac
     1921 ggctttcgga tcattgcagg aaatacttac caaatcctct gtcttttatt agccgtatta
     1981 ggattttgga ttgtcattaa gaatacagag ttttcaattg gtaaaaaccg acgctggttt
     2041 ggcggcatac tcttttattt cggtatctta ttattgttac atgcccattt atttggcaaa
     2101 ctccatactg gggaaccaaa tatcatgggg acgacatggg atttgttagc cagtgatata
     2161 aaacaaagtc aagtagacaa taacgttggt ggtgggatga ttggagcaat cctttatcat
     2221 tttacttact tcttgattgc acagcctgga agttatttag cagcagtgct attactggca
     2281 ggaggagcat tcctgttcag taatctggaa ggctatcagc tgttaaatgg tatccaatca
     2341 attggagaac gagtacagga attattggaa ggtgatcctg ccaagcaagc tcgaaagcaa
     2401 gcggcaaagg aagagagaat gaaacagcgc gcagaagcga aagaagcaag gcgcttagct
     2461 gcaaaagaag ctgcagaaaa agaagctgca gaatatgaga agaaaaaagc ggtgagccag
     2521 caaagggaaa atactgctga tgaatggcag gaaccagaaa atacagagcc ggagcagcta
     2581 agttttgttc caatcgacag ttttcaagaa aatatccatc ctgcgaatct agagaaacca
     2641 gtaccagata caccaaaaca gaccaataca gcagaaggtt tttcagatga gttgccagag
     2701 gatgacggta cctcattaga atttgagatt gaagcagaac aggaaaatca agactatgaa
     2761 ttaccgacag ttgatttgct tgattctata ccaactgtgg atcaaagtga tgagtataaa
     2821 aaaattgaaa aaaatatcgg cgtattagaa caaactttcc aaagtttcgg tgtcgatgcc
     2881 aaggtcgtca aagccagctt aggaccatcc gtcacaaaat tcgaagtcca gccggctgta
     2941 ggggtaaaag taagtaaaat tgtgaactta acagatgata tcgcgcttgc tttagcggcg
     3001 aaagatgtcc ggatggaagc accgattcca ggtaaatcac tgattggtat tgaagtacca
     3061 aatggaaaga tcagtatggt ttcctttaga gaaatcatcg aagcacaacc aaaccaccca
     3121 gataaattac tagaagtacc tttaggacgt gatgtttctg gacgggtaca aacggctgat
     3181 ctttcgaaga tgcctcactt actcgtggct ggttcgactg gtagtgggaa gtcagtagca
     3241 attaatggga ttatcacaag tatattaatg cgtgctaagc cccatgaagt aaaactgatg
     3301 atgatcgatc cgaaaatggt tgaattgaat atgtacaacg gcatacctca tctgttgacg
     3361 ccggtcgtga cgaaccctag aaaagcagcg caagccttac aaaaagtagt acaagaaatg
     3421 gaagaacgat atgaaaaatt cgcggcaacc ggagtaagaa atatttctgg ttacaatgaa
     3481 tttgttcaac agaaaaactt ggaaaacgga acgaaacatc caaccttacc attcatcgta
     3541 gtgatcgtag atgaattagc tgatttgatg atggtagcca gcaatgaagt tgaagatgcg
     3601 attattcgtt tagcgcaaat ggctcgggca gcggggattc atatgatcct agctactcaa
     3661 cgtccaagtg tggatgttat cacaggaatc atcaaagcaa atgtaccttc cagaatggca
     3721 tttgcggtat caagcggtac agattctcgg acgatcattg attctaatgg ggcagaaaag
     3781 ctcttagggc gaggagatat gctattcttg ccaatgggtg agaataaacc gatccgtgtg
     3841 caaggagcat tcatttctga ccacgaagta gaacgggtcg ttcaatttgt tacagatcag
     3901 caagaagctc attatgaaga gaaaatgatg ccgacagatg aagttgaaac gacaggtgct
     3961 ccagaacagc cacaagatga gctgtttgaa gaagcaaaag cacttgtcgt tgaaatgcaa
     4021 acggcaagta tttcccttct ccaaagaaga tttcggatcg gttacaaccg agcagcacgt
     4081 ttagtagatg aactggaagc tcatggagtt gttggcccgt cagaaggaag caagcctcgg
     4141 aaagtattca ttgagcagga agaaacatca gaaacgttgc cggacgataa tgacatgtaa
     4201 aacttgtgtg atgaagggtt gaaaaacgaa taaccaataa aaagacaagg catgatgata
     4261 ccaaaagaaa aaatggtttt tttccatctt ttctaatacg gtatgatgtc ttgtcttttt
     4321 agtttttgtg agattaaagt tctattcgat ttatttgtga agtattgcat taaggaaatg
     4381 aatcatgtat agtatgtagc gaaactttga gaaaaagagg gaaaatactt tggaaaaaca
     4441 gtccatttat ggcttgacga atgaagaatt aatcaattgg tttatagaaa acggcgagaa
     4501 aaaattccga gctgcccaag tttgggaatg gttgtatcaa aaacgtgtaa gtaatttcac
     4561 agaaatgacg aatctatcga aacaattgat cgaaaaatta tcggagcatt ttatcatcaa
     4621 tcctctaaaa cagatggtcg tccaagaagc ttcggatggt acagtaaaat atttgtttga
     4681 actccctgat aaaaacatga ttgagactgt actgatgaga caagaatatg gcttgtcagt
     4741 ttgtgtgacg acacaagtag gatgtaatat cggctgtacg ttttgtgcaa gcgggctgct
     4801 caaaaagaac cgtgatctga ctgctggaga aattgttgca cagatcatga tggtccaaca
     4861 ttattttgat gagcgcaatc ttggcgaacg cgtttcacat gtcgttgtaa tggggatcgg
     4921 tgagccattt gataattacg acaatgtaat ggactttttg catatcatca atgatgccaa
     4981 aggattggca attggtgctc ggcacattac agtttccact agtggattag cacacaaaat
     5041 caaggaattc gctaataacg gcttgcaagt caatctggct atttccttgc acgcgccgaa
     5101 taatgaggtg cgaacatcga tcatgcggat caaccgaagt ttcccaattg agaaattgat
     5161 ggaagctgtg gatgagtatt tagagaaaac aaaccgtcga atcacgtttg aatatatcat
     5221 gttgaatcaa gtaaatgata gaccagagca tgcgcaacaa ttagcagatc tattaaaaga
     5281 taagaaaaaa ttagcatatg tcaacttgat tccatataat ccagtcagtg aacatgatca
     5341 atattctcgc agtccaaaag ctgatgtatt gagattttat gatgtattga agaaaaacgg
     5401 cgtaaactgt gtgatacgta aagaacatgg tacagatatt gatgcagctt gcggacaatt
     5461 gcgaagcaag caaatgaaaa aaacagttgc caaataatcg tatcctactc gttttttcaa
     5521 ttaccataaa aggagcagtt ttcttaatta aggaactgtt caacttaaat aagagaaaag
     5581 attctatagc gaaagctata aagctttttt tgacaagaaa aaccctcatc aagcaaagaa
     5641 atgtcctcaa tttgatgaca gcctgcttga tgggggtttt agcaattatt taactgattt
     5701 tcttatatcc ttgctggctt tcttgtgtag catgataagt ttctacgtca agaatggcct
     5761 gtttaaattt ttctcgggtg atttcataag gcacatgttt aatatcattt gttgccatgg
     5821 ctttatctaa gattgcttct aaatcggtcg tgacattgag attcatgtcg gttaacgata
     5881 ctggaagttg gttctttttc ataaacacat agattttatc aaacaaatcg aactgctgat
     5941 ccatcatcaa taaggtcagt acaccataac tgacagatgc tccgtgaagg tatccctcca
     6001 ttttttctag aacagtcgta ccgtaataga atgagtgtgc catagcagaa ttatagttat
     6061 tgttaactaa aacagaaacc aatccggtag taacaatgat atccgaaacg acttgttcaa
     6121 ttgcatatga gggacgatcg tcggcactat ctttcaatgc ttgcgtacca taagtcaaga
     6181 gacgatcaat gcacattttt ccgatttgga ttcctaatct atccgtgtag cctagatcat
     6241 cgccacgagc agaaaaagta gattcgactt ctttggataa agcatcgcca atacctgccc
     6301 ataagtattt atcaggtgct tcggcaataa ttgctgtatc aatgaaggtg tgtacaggtg
     6361 gttttgaagg aaagaagagt ccgtccatta cgccttcttc gttatagatg acacataccg
     6421 cagtgacggg cgaacagttc gaaccaatag tgggaaaggt gaagactggt ttagctagtt
     6481 tttcaccaat gacttttaca gtatcgcaag cacggccacc accgactgca aacaggacgt
     6541 ctgctttttg gacactttca tgctggatca actgttcaat atttccataa gttgaattac
     6601 ccccgtacca gagactggcg acaacctcta tttcctgtgt caaagctact tttattttgt
     6661 tttctgcctt tgaaagtgca gtcttccctc caatcagcac tacttttgtc ccatattctt
     6721 tgacgattcc atttgctttt tcatacgcat ctgttccaat ggtataattt ggtaagaaaa
     6781 cactttgact catattcatt tccttctttc ttcgttgaaa cgggttaaaa ttttaaagag
     6841 tgatcgatct gactgcggct gtagatggag tagaggtgaa cctaagacag gataagtgat
     6901 gaataaggtg gcatgaacac cttgaaatca tcaataagta tcctaaaaac gaacaagtac
     6961 aaaaacattg aaaacgacaa taatgaattt ttgttcctct tagtatttct accgattccc
     7021 tttgcttttt tgtaattatc taaaaattct aaaaattaag attattatat gaaaacaggt
     7081 tttatttgtc aattcataat tggttttttt taaagcaaaa gataatctat atcatgatga
     7141 acaaacagag ttttttcctc caattgatgg ctttcctgat ctttcctatt ttgaataaag
     7201 tgtttctgta taattttttt attttataag taagataaat aatgaaagaa tagagatact
     7261 gattggtagc taaagatttt tgagcgagaa catttcgttt tttcaaaagg actgttagaa
     7321 taagcaagaa caagggggaa gacgtatgat aatcagtgaa tttgaccgta ataatccagt
     7381 attgaaagat cagctttctg atttactgag actgacttgg ccggaagaat atggagacag
     7441 ctcggcagaa gaagtagaag aaatgatgaa tccagaacga atcgcggtag cagcggtaga
     7501 ccaagatgag ttagtaggat ttattggtgc aatccctcaa tacggtatca caggttggga
     7561 attgcatcca ttagttgtag aaagctcccg acgaaagaac caaataggta ctcgattagt
     7621 caattactta gaaaaagaag tagcttccag aggaggaatc acgatttatt taggtacgga
     7681 tgatttagac catggaacaa cgttaagtca aacggacctg tatgagcata catttgataa
     7741 agtggcttcc atccagaacc ttcgtgaaca tccgtatgaa ttctatgaaa aattaggtta
     7801 taaaatcgta ggtgtcttac caaatgcaaa tggctgggac aaaccggata tttggatggc
     7861 aaaaacgatt attcctcgac caaattctca ataataaaaa gaggttgtga cagaagtaat
     7921 cagcttcaag aaataagaag gaatttacga aaattactt
//
LOCUS       DACGLW010000209         5851 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.guided.126, whole genome shotgun sequence.
ACCESSION   DACGLW010000209 DACGLW010000000
VERSION     DACGLW010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5851
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.guided.126"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            322..1014
                     /locus_tag="IXT53_003048"
     CDS             322..1014
                     /locus_tag="IXT53_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HAQ4906787.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
     gene            1341..1838
                     /gene="dfrG"
                     /locus_tag="IXT53_003049"
     CDS             1341..1838
                     /gene="dfrG"
                     /locus_tag="IXT53_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HAQ4906788.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(1910..3862)
                     /locus_tag="IXT53_003050"
     CDS             complement(1910..3862)
                     /locus_tag="IXT53_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HAQ4906789.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3869..4165)
                     /locus_tag="IXT53_003051"
     CDS             complement(3869..4165)
                     /locus_tag="IXT53_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906790.1"
                     /translation="MQIGVLKWLQFTKQKGVHFPMQFLEPKNKNAKSVDWEISEQVRV
                     IVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            4507..4797
                     /locus_tag="IXT53_003052"
     CDS             4507..4797
                     /locus_tag="IXT53_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906791.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            5511..5813
                     /locus_tag="IXT53_003053"
                     /pseudo
     CDS             5511..5813
                     /locus_tag="IXT53_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1997 a          925 c         1010 g         1919 t
ORIGIN      
        1 tttcataggt aaaagaagca tgacaattta tgacaaatcc gcgttttttg taataaatac
       61 agtaatctat gaaatgaaaa gggttacgtt taataaaact tgcttttttg tttttgttca
      121 ccataaatat ttatttaaga aagtattaac ctgcattttt ctctataaaa aagatttttt
      181 aaatagataa aaaaggagga ttaaatgagt atttgatagt atttatatta aaattacata
      241 tgtaatttaa aaacgcttga cattgtgttt gagatcactt acttttagca tgtaaaagta
      301 ttggattaag gagctatgca aatgaaaaag aaatttatta ctggtgggat tgtcgttgca
      361 tttctatgtc taattggcta cacaggcacg cggtacgcaa atgaaattat tttgtggggt
      421 ggagagacag atattgatac aatcaatgag aatctgacac agcttgatca tacattgaca
      481 gataaagagc aagcgattac agatcttacc gctcgactag cacaaaaaga acagacattg
      541 caagattcaa aggcagatgt ccaagaatac caaacaaaag taaatgaatt agaaaaccaa
      601 aaaaatcaat cggcagcaga aaaacaaaac ttagaaagtc aattagtcag caaggatgca
      661 caactacaaa gcaaacaaaa tgaaatcaat gtcaaactag atgagatcaa ccaaaaaaat
      721 gatgaaatca atcatctgaa tgcagattgg acagcaagac tacaagaagc acaagcaaaa
      781 ctttcagaat tacaaaatac tgtaaatggt ttgaatcaac aaatcgcaac actgacacaa
      841 gaaaaaacaa atgtagaaac tcagttaaca gatacacaaa agaaattaga cgaagcaact
      901 gctgaaaact caagcttgaa acaatatatt caaaagttag aaaaagcaaa agatgaagtg
      961 gaagatacag ctgataaatc tcagcaaatt gttgaagaac atttgcgtaa ataatctttc
     1021 ttaacaagta aacctaaatt agtaaagaag caacagtaat ctatactaag tagttcagtt
     1081 ttggagtaca aaatgacaaa atgtacttca aaatggacta cttttttcat tttaacttac
     1141 ataccaaatt acatacaaaa tgatttataa aatgcaattg aaaatgataa ttgaatgaaa
     1201 atttgtgatt ttgtggtatt atgataatat aacttataga aaaaattgct tattcaacta
     1261 acgggcagga taatgagaaa aggttttttg atagaaataa gaaattagat ggtaaattag
     1321 ttaaaaaggc tgtgttataa atgaaagttt ctttgattgc tgcgatggat aagaatagag
     1381 tgattggcaa agagaatgac attccttgga ggattcccaa ggactgggaa tatgttaaaa
     1441 atactacaaa gggacatccg ataatattag gtaggaagaa ccttgaatca atcggaagag
     1501 ccttacctga cagaagaaat attattctga cgagagataa ggggtttacc tttaatggtt
     1561 gtgaaattgt tcattcaata gaagatgttt ttgagttatg taaaaacgaa gaagaaattt
     1621 ttattttcgg aggagaacag atttataatt tgtttttccc ttatgttgag aaaatgtaca
     1681 tcacaaaaat acatcatgaa ttcgaaggag atactttttt tccagaagtg aattatgagg
     1741 aatggaatga ggtatttgcc caaaaaggga taaagaatga taaaaatccg tataactact
     1801 attttcatgt atatgaaaga aaaaacttat tgagttaacg ggtgcgttaa ttgaagaaga
     1861 acagtaaaga taaagcacaa gagcttggga ttactccaag ctctttttgt catttgaaat
     1921 aaattatatc tttccaatca aattgtttat ctgtaatccc taatctttga gcaggtgtca
     1981 aaaccttttt atccgctgat ttaaaagggc gacaaaagtt gtagtaagtt cttagaattg
     2041 taatcgcaaa ttgagcatac ttcggattga agttagcgta aatatagctc ttcttatcac
     2101 cacgagctgt tgttaatggt ctttcaagag atgatatata acgtcttatc tgttgaataa
     2161 agctattggt cgaatggtca ttaacattta atagcatctt tgcgatttcg tttggttcca
     2221 gagcagataa atccgttcta caatcaactg aataaaatcc tttatcctta gtggctaatg
     2281 gatgtttgat agggttatcg gcatatttgc gatatctttc tccatcttta ctaatagcct
     2341 cttcgtggaa ttgatgactt gttagtaatt cagttagata atgagatgcc aaagtccgta
     2401 gaaactttgt tttgaatcct ctgatatctc cccaatcgag cagttctgct tttgcttgtc
     2461 cgaattcttt taagcattgt tttctgcttt tcgtcttgtt aacttggcaa ataaaatgat
     2521 gtgcatcgct caaccttaat tctttcgtga atactctata aaaggcgttt ctaatagaga
     2581 agtcatcatc actaatcatt cgccattcgt cggcattaac catttgttta attaaccaat
     2641 agtgagcagt tgtagtataa ggagcatcaa catttaaacc atcgacaaat ctactcctat
     2701 tagtaatttt ccctaattcg tgaagataag cattcctatt ttcgctatcg ttagcggatg
     2761 gttcttgtgg ataataagac caatctaacc tatcattttt acgactaaaa gtattgagat
     2821 ggtcttcttt gaattttctt gtatcttcat ttaattcatc cattgaaata ttccaatcat
     2881 aggcaacatc agaacggaac acatatcttg ataagacctc agcagataca actatatacg
     2941 tttgcatatt aaggtcttca aaccccgtat atttctttga accttgtcct ttcttacgga
     3001 cgttatttag gtagtagtgc atcttgtccg tattaagcca catctcgtta aatttctttg
     3061 tttgaagggg ttgtgtctca tatcgttcta aaaactccaa acaacgccta tacagccact
     3121 ccagcttatg atagtaggtt ccaactccaa ttcctaaaat atcacaagta cgactgactg
     3181 aaacccttcc aacaaccatc ttagcgaaca ttggaagaat ggtgttcttt tgttggtgat
     3241 aagtagtggt ttcttctctt tttggtagaa cattagtgaa cttcttacac gccttacatt
     3301 gatagcgttg agatttgccc ctacttttcc cccttttgta aaattgcttc ggctcattga
     3361 atggggttga ttcttcttcc gagcagcctt ctttatgaaa ttgataatct ggctcaacat
     3421 cttggatgct attgatttct ataaggcgtt taatttcttc aacaacagac caattagata
     3481 aaggggttac tgtacagttt tgcgatacac ctctgccaat aggattgtca ttgcagtaca
     3541 tacctttatg acctttatct ttggaactac cagtcatttt atatcgactt ggtttccctt
     3601 ttacatcttt gaatttgtgc tgttctttcc caaaattaca acaaaacgga ttgatgcagt
     3661 aattatattg aatgggatat tcagaaccgt tccatttaaa agacgttggc aagaacaaca
     3721 ttttgttata ccttgttgca aatcgttttg gagttaataa atcatattgt ttagccctat
     3781 cttttaattc ttcgtttgaa atcggaattt taacatctac aatttcatct tccttcgtgg
     3841 ctaatctttt aagcttggtc attgtctatc aacccactct atctttgata cccatttttt
     3901 caacaatccg cttattactt cgcttgttgg caatccattc aataaacttc ttatcgtcta
     3961 aaatgttcaa taagaattca tccactgctt cgctttcagt acgctctgca tattcagcgt
     4021 attgcttaac tatcaccctt acttgctctg atatttccca atccacagac ttagcgttct
     4081 tattttttgg ctcaagaaat tgcataggaa aatgcacccc tttttgctta gtaaattgta
     4141 accattttaa cacaccaatt tgcattccta caataattat ttcacgttcg catttttata
     4201 tacattttaa cttacatttt gtggtgtaaa ttaacaacaa acgttgattt aacaataaaa
     4261 aacgagtatc aaattcagtt gcaatttgat actcatttag gtttacattt tcactttttg
     4321 tactccaaaa ctgaactact taaatctata ctgctgcctc ttttttttag tacatgtcct
     4381 tcatcttcgc aggaagtgcc aatatgattt taagaaaggt aatagacaaa aagtcaaaaa
     4441 gataaaacag agtttcttct ttacaaaggg aatgtatgtt cgtataatgg tattgaggtg
     4501 aaggttatgg agaaaaaata tggaacagta gataagatcc gtactttgca agttagtgcc
     4561 agaccgttga tccgtttcag tttaaatggg gtcaattgtt tgattgcttc ccatagtttg
     4621 aacttcctag ctgaagtaga cgaagggatg aagttagtcg ttacaggtta ttataataat
     4681 agaaagcaat ttgttgtgag atcctatcat gtgttaggga aacccaaaat tgtggtagaa
     4741 tatgagaaaa gtctataccc aagaaaaaag gtccgacaag atgaaagaat aaactagaaa
     4801 acaagcggct gagacaaaaa tgtcctagcc gcttgttttg gaaagatgga tgcagacgat
     4861 gggatttgaa cccacacgag catacgctca caagaacctc aatctatata taactacatg
     4921 tatttaagta tttgtgatga atcactaaac tttccaaact catttgaggg atattgctta
     4981 aagattgaac agatcaattc gaagccctat tagtattcgc tcaatggggg gatcagaggc
     5041 cataaaaaga gactagtcat tactagtctc aggtagtgga attgtaggat tttaaacgtt
     5101 cctggagagt accggtatgt aactcgaaga ggtgattatc atagtcgtaa aagtaaattg
     5161 aagaagcctc gcctttcaca cgagaacgtc ctggttcgac agttaaacct aacatttgga
     5221 tttttgagac aaacgaatct atatcttgtt cgtcaatttt aaaagcaata tgattgtatg
     5281 tctttggcag tttattcgac gaattctgca ttactgcaat ccataggtcc ttaactaaga
     5341 aaaatttttc tggatataat gaaaattttt ttgttgacta tcgtagattt cttttgcatc
     5401 aaataaggta tttaacaatt aagtatttaa aagataactt ttaagttatc tttaagtaat
     5461 gcttaaagct atgttatact ctattaggaa atataagaag gagtataaaa atgaacaaac
     5521 ataatagaaa aataacgaat aacaaagaac tgatcttgtt actaataggg atttggggta
     5581 tctttttact ctttaatatg tatatgccga ctatgagagc ggatgacgta gtttatgcaa
     5641 gtagattaga tgaacttggc tatttgggtg cttcgataga acattataag acatggagtt
     5701 caagaattat tatagaacta ttcttaatgt tcttttctaa acactttatg ctatggaaat
     5761 tattaaattc tacgattatg ttaggaattg tagtattact ttgcaagtat gttgagagta
     5821 taaaatattt tgtgtaaatg aaaaaatcca t
//
LOCUS       DACGLW010000210         5774 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.guided.127, whole genome shotgun sequence.
ACCESSION   DACGLW010000210 DACGLW010000000
VERSION     DACGLW010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5774
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.guided.127"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            complement(85..3009)
                     /locus_tag="IXT53_003054"
     CDS             complement(85..3009)
                     /locus_tag="IXT53_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HAQ4906792.1"
                     /translation="MKIARGRELLTPEQRQAFMQIPEDEWILGTYFTFSKRDLEIVNK
                     RRREENRLGFAVQLAVLRYPGWPYTHIKSIPDSVIQYISKQIGVSPSSLDHYPQRENT
                     LWDHLKEIRSEYDFVTFTLSEYRMTFKYLHQLALENGDAIHLLHECIDFLRKNKIILP
                     AITTLERMVWEARAMAEKKLFNTVSKSLTNEQKEKLEGIITSQHPSESNKTILGWLKE
                     PPGHPSPETFLKIIERLEYIRGMDLETVQISHLHRNRLLQLSRLGSRYEPYAFRDFQE
                     NKRYSILTIYLLQLTQELTDKAFEIHDRQILSLLSKGRKAQEEIQKQNGKKLNEKVIH
                     FTNIGQALIKAREEKLDVFKVLESVIEWNTFVSSVEEAQELARPADYDYLDLLQKRFY
                     SLRKYTPTLLRVLEFHSTKANEPLLQAVEIIRGMNESGKRKVPDDSPVDFISKRWKRH
                     LYEDDGTTINRHYYEMAVLTELREHVRAGDVSIVGSRQYRDFEEYLFSEDTWNQSKGN
                     TRLSVSLSFEDYITERTSSFNERLKWLAANSNKLDGVSLEKGKLSLARLEKDVPEEAK
                     KFSASLYQMLPRIKLTDLLMDVAHITGFHEQFTHASNNRKPDKEETIIIMAALLGMGM
                     NIGLSKMAEATPGLTYKQLANVSQWRMYEDAMNKAQAILVNFHHKLQLPFYWGDGTTS
                     SSDGMRMQLGVSSLHADANPHYGTGKGATIYRFTSDQFSSYYTKIIHTNSRDAIHVLD
                     GLLHHETDLNIEEHYTDTAGYTDQIFGLTHLLGFKFAPRIRDLSDSKLFTIDKASEYP
                     KLEAILRGQINTKVIKENYEDVLRLAHSIREGTVSASLIMGKLGSYSRQNSLATALRE
                     MGRIEKTIFILNYISDESLRRKIQRGLNKGEAMNGLARAIFFGKQGELRERTIQHQLQ
                     RASALNIIINAISIWNTLHLTTAVEYKKRTGSFNEDLLHHMSPLGWEHINLLGEYHFN
                     SE"
     gene            3155..3730
                     /locus_tag="IXT53_003055"
     CDS             3155..3730
                     /locus_tag="IXT53_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016204422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HAQ4906793.1"
                     /translation="MRKIGYIRVSSTNQNPSRQFQQLNEIGMDIIYEEKVSGATKDRE
                     QLQKVLDDLQEDDIIYVTDLTRITRSTQDLFELIDNIRDKKASLKSLKDTWLDLSEDN
                     PYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKL
                     YKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            3944..4639
                     /gene="vanR"
                     /locus_tag="IXT53_003056"
     CDS             3944..4639
                     /gene="vanR"
                     /locus_tag="IXT53_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HAQ4906794.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            4617..5768
                     /locus_tag="IXT53_003057"
     CDS             4617..5768
                     /locus_tag="IXT53_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000791889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HAQ4906795.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPGFCCKVLNKE"
BASE COUNT         1782 a         1090 c         1089 g         1813 t
ORIGIN      
        1 ggattttatt ctttatttaa aactttgcaa cagaaccgtc taaaatactt tgttttttta
       61 cctgtctact tgacaggggg cagttcactc tgagttaaaa tggtattctc ctagtaaatt
      121 aatatgttcc caacctaagg gcgacatatg gtgtaacaaa tcttcattaa agctacctgt
      181 ccgtttttta tattcaactg ctgttgttag gtggagagta ttccaaatac ttatagcatt
      241 gataattatg tttaaagcac tggctctttg caattgatgc tgtatggtgc gttctctaag
      301 ctcaccttgt tttccgaaga aaatagctct tgccaatcca ttcatggctt ctcctttatt
      361 caatcctctt tgtatttttc ttcttaatga ttcatccgat atataattca aaataaagat
      421 cgttttttct attcggccca tctcacgtaa ggctgtagct aagctgtttt gtcttgaata
      481 ggaacctagc ttccccataa taagggatgc tgaaactgtt ccctccctta tagaatgagc
      541 taatcgcaaa acatcctcat aattttcttt aatgaccttt gtatttattt gtccacgtaa
      601 aatggcttct agttttggat actcacttgc tttatctatc gtaaataatt ttgagtccga
      661 taaatccctt attcttgggg caaatttaaa tcctaataaa tgagtcagtc cgaatatttg
      721 gtcagtgtaa ccggcagtgt ctgtataatg ttcctctatg tttagatccg tctcatgatg
      781 taacaaacca tccaaaacat gaatcgcatc tcttgaatta gtatgaataa tctttgtgta
      841 gtaagaagag aattgatcac ttgtaaatcg gtagatggtg gctccttttc cagttccata
      901 atgtggattt gcatctgcat gtagtgatga aacacctagc tgcattctca taccatctga
      961 cgaagatgtt gtaccgtcgc cccaatagaa aggcaattgt aatttatgat gaaagtttac
     1021 taatatggct tgggctttat tcatggcatc ttcatacatg cgccattgag atacattggc
     1081 tagttgctta tatgtaagtc cgggtgtggc ttcggccatc ttgctcaagc caatattcat
     1141 tcccattcct aaaagggcag ccatgataat gattgtttct tccttatctg gttttcgatt
     1201 attggaagca tgagtgaatt gctcatgaaa tcctgttata tgggccacat ccatgagtaa
     1261 atcagttaat tttattcttg gtagcatctg ataaaggctt gcactaaatt tttttgcttc
     1321 ttctggaaca tctttttcta agcgtgcaag tgatagcttt cctttttcaa gagaaacccc
     1381 atctaactta ttggaattgg cagctaacca ctttaacctt tcattaaagc tgctggttct
     1441 ctccgttata taatcttcga atgataaact aactgataat ctcgtattcc ccttcgattg
     1501 attccatgta tcttccgaaa acaaatattc ctcaaaatcc ctatattgtc tgctgccaac
     1561 aatggaaaca tctcctgccc gaacatgctc ccgaagttct gttaaaacag ccatttcata
     1621 gtaatgacga ttaattgttg taccatcatc ctcgtataaa tgtcttttcc atcgttttga
     1681 aataaaatcc acaggtgagt catcaggcac ttttcgcttt ccagattcgt tcattcctcg
     1741 gataatctca acagcttgta aaagtggctc atttgccttt gtagaatgaa attccaatac
     1801 tcttaatagc gttggcgtat attttcttag tgaataaaac cgtttttgca gtaagtctaa
     1861 ataatcatag tcggcaggac gtgcaagttc ctgagcctct tctactgaag agacaaaggt
     1921 attccattca ataaccgatt ctaaaacctt aaaaacgtct aatttttcct ctcttgcttt
     1981 aattaatgct tgtccgatgt tcgtaaagtg tataactttc tcatttagct ttttaccgtt
     2041 ttgtttctgg atttcctctt gagccttacg accttttgat aacaaactaa gtatttgcct
     2101 atcatgaatt tcaaacgctt tatccgttag ctcctgagta agttgtaata aatagatggt
     2161 taatatcgaa taacgtttat tttcttgaaa gtcacggaat gcatacggct cgtatcttga
     2221 gcctaagcga gacagctgca acaggcggtt acggtgcaaa tgactaattt gcactgtttc
     2281 taaatccatt cctcgtatgt attcgagtcg ttctattatt tttagaaaag tttcgggtga
     2341 aggatgaccc ggtggctctt ttaaccaacc caatatcgtt ttattggatt cggatggatg
     2401 ctgcgaggta ataatccctt caagcttttc tttttgctca tttgttagag atttactaac
     2461 cgtattaaat agcttctttt cagccattgc ccttgcttcc cacaccattc tttcaagtgt
     2521 agtgatagca ggcagtataa ttttgttttt tcttagaaaa tctatgcatt catgcagtag
     2581 atgaatggca tcaccatttt ccaaagctaa ttgatgaagg tacttaaatg tcattcgata
     2641 ttcactcagg gtaaaagtta caaagtcgta ttcacttcga atttctttca aatgatccca
     2701 aagtgtattt tccctttgag gataatgatc aagcgaggat ggactaacac caatctgttt
     2761 cgatatatat tgtatgaccg aatctgggat gcttttgata tgagtgtatg gccaaccggg
     2821 ataccgaaga acagctaatt gaacagcaaa tcctaaacgg ttttcttccc tccttcgctt
     2881 attaactatt tctaaatccc gtttggaaaa agtgaagtag gtccccagta tccattcatc
     2941 ttcagggatt tgcataaaag cctgtctctg ttccggtgta agcaattctc tacctctcgc
     3001 aattttcatt cagtatcatt ccatttctgt attttcaatt tattagttca attatatatc
     3061 aatagagtgt actctattga tacaaatgta gtagactgat aaaatcatag ttaagagcgt
     3121 ctcataagac ttgtctcaaa aatgaggtga tattttgcgg aaaatcggtt atattcgtgt
     3181 cagttcgact aaccagaatc cttcaagaca atttcagcag ttgaacgaga tcggaatgga
     3241 tattatatat gaagagaaag tttcaggagc aacaaaggat cgcgagcaac ttcaaaaagt
     3301 gttagacgat ttacaggaag atgacatcat ttatgttaca gacttaactc gaatcactcg
     3361 tagtacacaa gatctatttg aattaatcga taacatacga gataaaaagg caagtttaaa
     3421 atcactaaaa gatacatggc ttgatttatc agaagataat ccatacagcc aattcttaat
     3481 tactgtaatg gctggtgtta accaattaga gcgagatctt attcggatga gacaacgtga
     3541 agggattgaa ttggctaaga aagaaggaaa gtttaaaggt cgattaaaga agtatcataa
     3601 aaatcacgca ggaatgaatt atgcggtaaa gctatataaa gaaggaaata tgactgtaaa
     3661 tcaaatttgt gaaattacta atgtatctag ggcttcatta tacaggaaat tatcagaagt
     3721 gaataattag ccattctgta ttccgctaat gggcaatatt tttaaagaag aaaaggaaac
     3781 tataaaatat taacagcctc ctagcgatgc cgaaaagccc tttgataaaa aaagaatcat
     3841 catcttaaga aattcttagt catttattat gtaaatgctt ataaattcgg ccctataatc
     3901 tgataaatta ttaagggcaa acttatgtga aagggtgata actatgagcg ataaaatact
     3961 tattgtggat gatgaacatg aaattgccga tttggttgaa ttatacttaa aaaacgagaa
     4021 ttatacggtt ttcaaatact ataccgccaa agaagcattg gaatgtatag acaagtctga
     4081 gattgacctt gccatattgg acatcatgct tcccggcaca agcggcctta ctatctgtca
     4141 aaaaataagg gacaagcaca cctatccgat tatcatgctg accgggaaag atacagaggt
     4201 agataaaatt acagggttaa caatcggcgc ggatgattat ataacgaagc cctttcgccc
     4261 actggagtta attgctcggg taaaggccca gttgcgccga tacaaaaaat tcagtggagt
     4321 aaaggagcag aacgaaaatg ttatcgtcca ctccggcctt gtcattaatg ttaacaccca
     4381 tgagtgttat ctgaacgaga agcagttatc ccttactccc accgagtttt caatactgcg
     4441 aatcctctgt gaaaacaagg ggaatgtggt tagctccgag ctgctatttc atgagatatg
     4501 gggcgacgaa tatttcagca agagcaacaa caccatcacc gtgcatatcc ggcatttgcg
     4561 cgaaaaaatg aacgacacca ttgataatcc gaaatatata aaaacggtat ggggggttgg
     4621 ttataaaatt gaaaaataaa aaaaacgact attccaaact agaacgaaaa ctttacatgt
     4681 atatcgttgc aattgttgtg gtagcaattg tattcgtgtt gtatattcgt tcaatgatcc
     4741 gagggaaact tggggattgg atcttaagta ttttggaaaa caaatatgac ttaaatcacc
     4801 tggacgcgat gaaattatat caatattcca tacggaacaa tatagatatc tttatttatg
     4861 tggcgattgt cattagtatt cttattctat gtcgcgtcat gctttcaaaa ttcgcaaaat
     4921 actttgacga gataaatacc ggcattgatg tacttattca gaacgaagat aaacaaattg
     4981 agctttctgc ggaaatggat gttatggaac aaaagctcaa cacattaaaa cggactctgg
     5041 aaaagcgaga gcaggatgca aagctggccg aacaaagaaa aaatgacgtt gttatgtact
     5101 tggcgcacga tattaaaacg ccccttacat ccattatcgg ttatttgagc ctgcttgacg
     5161 aggctccaga catgccggta gatcaaaagg caaagtatgt gcatatcacg ttggacaaag
     5221 cgtatcgact cgaacagcta atcgacgagt tttttgagat tacacggtat aacctacaaa
     5281 cgataacgct aacaaaaacg cacatagacc tatactatat gctggtgcag atgaccgatg
     5341 aattttatcc tcagctttcc gcacatggaa aacaggcggt tattcacgcc cccgaggatc
     5401 tgaccgtgtc cggcgaccct gataaactcg cgagagtctt taacaacatt ttgaaaaacg
     5461 ccgctgcata cagtgaggat aacagcatca ttgacattac cgcgggcctc tccggggatg
     5521 tggtgtcaat cgaattcaag aacactggaa gcatcccaaa agataagcta gctgccatat
     5581 ttgaaaagtt ctataggctg gacaatgctc gttcttccga tacgggtggc gcgggacttg
     5641 gattggcgat tgcaaaagaa attattgttc agcatggagg gcagatttac gcggaaagca
     5701 atgataacta tacgacgttt agggtagagc ttccagggtt ctgttgcaaa gttttaaata
     5761 aagaataaaa tccc
//
LOCUS       DACGLW010000211         2950 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.guided.157, whole genome shotgun sequence.
ACCESSION   DACGLW010000211 DACGLW010000000
VERSION     DACGLW010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2950
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.guided.157"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            297..1265
                     /locus_tag="IXT53_003058"
     CDS             297..1265
                     /locus_tag="IXT53_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HAQ4906796.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1258..2289
                     /gene="vanA"
                     /locus_tag="IXT53_003059"
     CDS             1258..2289
                     /gene="vanA"
                     /locus_tag="IXT53_003059"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HAQ4906797.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2295..2903
                     /locus_tag="IXT53_003060"
     CDS             2295..2903
                     /locus_tag="IXT53_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HAQ4906798.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT          876 a          563 c          714 g          797 t
ORIGIN      
        1 ccttatttga tagtagattt aaaactttgc aacagaaccg cttccagcga tgccagactt
       61 ggttgataaa aggaggtcct aagagatgta tataattttt taggaaaatc tcaaggttat
      121 ctttactttt tcttaggaaa ttaacaattt aatattaaga aacggctcgt tcttacacgg
      181 tagacttaat accgtaagaa cgagccgttt tcgttcttca gagaaagatt tgacaagatt
      241 accattggca tccccgtttt atttggtgcc tttcacagaa agggttggtc ttaattatga
      301 ataacatcgg cattactgtt tatggatgtg agcaggatga ggcagatgca ttccatgctc
      361 tttcgcctcg ctttggcgtt atggcaacga taattaacgc caacgtgtcg gaatccaacg
      421 ccaaatccgc gcctttcaat caatgtatca gtgtgggaca taaatcagag atttccgcct
      481 ctattcttct tgcgctgaag agagccggtg tgaaatatat ttctacccga agcatcggct
      541 gcaatcatat agatacaact gctgctaaga gaatgggcat cactgtcgac aatgtggcgt
      601 actcgccgga tagcgttgcc gattatacta tgatgctaat tcttatggca gtacgcaacg
      661 taaaatcgat tgtgcgctct gtggaaaaac atgatttcag gttggacagc gaccgtggca
      721 aggtactcag cgacatgaca gttggtgtgg tgggaacggg ccagataggc aaagcggtta
      781 ttgagcggct gcgaggattt ggatgtaaag tgttggctta tagtcgcagc cgaagtatag
      841 aggtaaacta tgtaccgttt gatgagttgc tgcaaaatag cgatatcgtt acgcttcatg
      901 tgccgctcaa tacggatacg cactatatta tcagccacga acaaatacag agaatgaagc
      961 aaggagcatt tcttatcaat actgggcgcg gtccacttgt agatacctat gagttggtta
     1021 aagcattaga aaacgggaaa ctgggcggtg ccgcattgga tgtattggaa ggagaggaag
     1081 agtttttcta ctctgattgc acccaaaaac caattgataa tcaattttta cttaaacttc
     1141 aaagaatgcc taacgtgata atcacaccgc atacggccta ttataccgag caagcgttgc
     1201 gtgataccgt tgaaaaaacc attaaaaact gtttggattt tgaaaggaga caggagcatg
     1261 aatagaataa aagttgcaat actgtttggg ggttgctcag aggagcatga cgtatcggta
     1321 aaatctgcaa tagagatagc cgctaacatt aataaagaaa aatacgagcc gttatacatt
     1381 ggaattacga aatctggtgt atggaaaatg tgcgaaaaac cttgcgcgga atgggaaaac
     1441 gacaattgct attcagctgt actctcgccg gataaaaaaa tgcacggatt acttgttaaa
     1501 aagaaccatg aatatgaaat caaccatgtt gatgtagcat tttcagcttt gcatggcaag
     1561 tcaggtgaag atggatccat acaaggtctg tttgaattgt ccggtatccc ttttgtaggc
     1621 tgcgatattc aaagctcagc aatttgtatg gacaaatcgt tgacatacat cgttgcgaaa
     1681 aatgctggga tagctactcc cgccttttgg gttattaata aagatgatag gccggtggca
     1741 gctacgttta cctatcctgt ttttgttaag ccggcgcgtt caggctcatc cttcggtgtg
     1801 aaaaaagtca atagcgcgga cgaattggac tacgcaattg aatcggcaag acaatatgac
     1861 agcaaaatct taattgagca ggctgtttcg ggctgtgagg tcggttgtgc ggtattggga
     1921 aacagtgccg cgttagttgt tggcgaggtg gaccaaatca ggctgcagta cggaatcttt
     1981 cgtattcatc aggaagtcga gccggaaaaa ggctctgaaa acgcagttat aaccgttccc
     2041 gcagaccttt cagcagagga gcgaggacgg atacaggaaa cggcaaaaaa aatatataaa
     2101 gcgctcggct gtagaggtct agcccgtgtg gatatgtttt tacaagataa cggccgcatt
     2161 gtactgaacg aagtcaatac tctgcccggt ttcacgtcat acagtcgtta tccccgtatg
     2221 atggccgctg caggtattgc acttcccgaa ctgattgacc gcttgatcgt attagcgtta
     2281 aaggggtgat aagcatggaa ataggattta cttttttaga tgaaatagta cacggtgttc
     2341 gttgggacgc taaatatgcc acttgggata atttcaccgg aaaaccggtt gacggttatg
     2401 aagtaaatcg cattgtaggg acatacgagt tggctgaatc gcttttgaag gcaaaagaac
     2461 tggctgctac ccaagggtac ggattgcttc tatgggacgg ttaccgtcct aagcgtgctg
     2521 taaactgttt tatgcaatgg gctgcacagc cggaaaataa cctgacaaag gaaagttatt
     2581 atcccaatat tgaccgaact gagatgattt caaaaggata cgtggcttca aaatcaagcc
     2641 atagccgcgg cagtgccatt gatcttacgc tttatcgatt agacacgggt gagcttgtac
     2701 caatggggag ccgatttgat tttatggatg aacgctctca tcatgcggca aatggaatat
     2761 catgcaatga agcgcaaaat cgcagacgtt tgcgctccat catggaaaac agtgggtttg
     2821 aagcatatag cctcgaatgg tggcactatg tattaagaga cgaaccatac cccaatagct
     2881 attttgattt ccccgttaaa taaactttta acggttctgt tgcaaagttt taaataaaga
     2941 ataaaatccc
//
LOCUS       DACGLW010000212         1781 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.guided.167, whole genome shotgun sequence.
ACCESSION   DACGLW010000212 DACGLW010000000
VERSION     DACGLW010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.guided.167"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            24..416
                     /locus_tag="IXT53_003061"
     CDS             24..416
                     /locus_tag="IXT53_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HAQ4906799.1"
                     /translation="MKLLFIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            417..>1781
                     /gene="aph(2'')-I"
                     /locus_tag="IXT53_003062"
     CDS             417..>1781
                     /gene="aph(2'')-I"
                     /locus_tag="IXT53_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(2'')-Ia/If/Ih family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HAQ4906800.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYDGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKE
                     IKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYIL
                     LRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGII
                     DFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPI
                     ETIVY"
BASE COUNT          731 a          136 c          303 g          611 t
ORIGIN      
        1 tatagttgaa gaatattatc ctattgaaac tattgtttat tgatgataat atggtttttg
       61 atattgataa tttaaaagga tttcttaatg ataccagttc atttgggttt atagctaaag
      121 aaaataataa aattatagga tttgcatatt gctatacact tttaagacct gatggaaaaa
      181 caatgtttta tttacactca ataggaatgt tacctaacta tcaagacaaa ggttatggtt
      241 caaaattatt atcttttatt aaggaatatt ctaaagagat tggttgttct gaaatgtttt
      301 taataactga taaaggtaat cctagagctt gccatgtata tgaaaaatta ggtggtaaaa
      361 atgattataa agatgaaata gtatatgtat atgattatga aaaaggtgat aaataaatga
      421 atatagttga aaatgaaata tgtataagaa ctttaataga tgatgatttt cctttgatgt
      481 taaaatggtt aactgatgaa agagtattag aattttatga tggtagagat aaaaaatata
      541 cattagaatc attaaaaaaa cattatacag agccttggga agatgaagtt tttagagtaa
      601 ttattgaata taacaatgtt cctattggat atggacaaat atataaaatg tatgatgagt
      661 tatatactga ttatcattat ccaaaaactg atgagatagt ctatggtatg gatcaattta
      721 taggagagcc aaattattgg agtaaaggaa ttggtacaag atatattaaa ttgatttttg
      781 aatttttgaa aaaagaaaga aatgctaatg cagttatttt agaccctcat aaaaataatc
      841 caagagcaat aagggcatac caaaaatctg gttttagaat tattgaagat ttgccagaac
      901 atgaattaca cgagggcaaa aaagaagatt gttatttaat ggaatataga tatgatgata
      961 atgccacaaa tgttaaggca atgaaatatt taattgagca ttactttgat aatttcaaag
     1021 tagatagtat tgaaataatc ggtagtggtt atgatagtgt ggcatattta gttaataatg
     1081 aatacatttt taaaacaaaa tttagtacta ataagaaaaa aggttatgca aaagaaaaag
     1141 caatatataa ttttttaaat acaaatttag aaactaatgt aaaaattcct aatattgaat
     1201 attcgtatat tagtgatgaa ttatctatac taggttataa agaaattaaa ggaacttttt
     1261 taacaccaga aatttattct actatgtcag aagaagaaca aaatttgtta aaacgagata
     1321 ttgccagttt tttaagacaa atgcacggtt tagattatac agatattagt gaatgtacta
     1381 ttgataataa acaaaatgta ttagaagagt atatattgtt gcgtgaaact atttataatg
     1441 atttaactga tatagaaaaa gattatatag aaagttttat ggaaagacta aatgcaacaa
     1501 cagtttttga gggtaaaaag tgtttatgcc ataatgattt tagttgtaat catctattgt
     1561 tagatggcaa taatagatta actggaataa ttgattttgg agattctgga attatagatg
     1621 aatattgtga ttttatatac ttacttgaag atagtgaaga agaaatagga acaaattttg
     1681 gagaagatat attaagaatg tatggaaata tagatattga gaaagcaaaa gaatatcaag
     1741 atatagttga agaatattat cctattgaaa ctattgttta t
//
LOCUS       DACGLW010000213         1532 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.guided.173, whole genome shotgun sequence.
ACCESSION   DACGLW010000213 DACGLW010000000
VERSION     DACGLW010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.guided.173"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            228..1103
                     /locus_tag="IXT53_003063"
                     /note="aph(2'')-Ia"
     CDS             228..1103
                     /locus_tag="IXT53_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021424053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HAQ4906801.1"
                     /translation="MKYLIEHYFDNFKVDSIEIIGSGYDSVAYLVNNEYIFKTKFSTN
                     KKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYSTM
                     SEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYILLRETIYNDLTDIEK
                     DYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDF
                     IYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPIETIVYGIKNIKQEF
                     IENGRKEIYKRTYKD"
     gene            complement(1233..>1532)
                     /locus_tag="IXT53_003064"
     CDS             complement(1233..>1532)
                     /locus_tag="IXT53_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015077585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HAQ4906802.1"
                     /translation="LFDSRFKTLQQNQPKYSKACASLDDGFEDAFQYTVQGNSHNRLK
                     STNLIERLNQEVRRREKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
BASE COUNT          610 a          144 c          259 g          519 t
ORIGIN      
        1 gtacaagata tattaaattg atttttgaat ttttgaaaaa agaaagaaat gctaatgcag
       61 ttattttaga ccctcataaa aataatccaa gagcaataag ggcataccaa aaatctggtt
      121 ttagaattat tgaagatttg ccagaacatg aattacacga gggcaaaaaa gaagattgtt
      181 atttaatgga atatagatat gatgataatg ccacaaatgt taaggcaatg aaatatttaa
      241 ttgagcatta ctttgataat ttcaaagtag atagtattga aataatcggt agtggttatg
      301 atagtgtggc atatttagtt aataatgaat acatttttaa aacaaaattt agtactaata
      361 agaaaaaagg ttatgcaaaa gaaaaagcaa tatataattt tttaaataca aatttagaaa
      421 ctaatgtaaa aattcctaat attgaatatt cgtatattag tgatgaatta tctatactag
      481 gttataaaga aattaaagga acttttttaa caccagaaat ttattctact atgtcagaag
      541 aagaacaaaa tttgttaaaa cgagatattg ccagtttttt aagacaaatg cacggtttag
      601 attatacaga tattagtgaa tgtactattg ataataaaca aaatgtatta gaagagtata
      661 tattgttgcg tgaaactatt tataatgatt taactgatat agaaaaagat tatatagaaa
      721 gttttatgga aagactaaat gcaacaacag tttttgaggg taaaaagtgt ttatgccata
      781 atgattttag ttgtaatcat ctattgttag atggcaataa tagattaact ggaataattg
      841 attttggaga ttctggaatt atagatgaat attgtgattt tatatactta cttgaagata
      901 gtgaagaaga aataggaaca aattttggag aagatatatt aagaatgtat ggaaatatag
      961 atattgagaa agcaaaagaa tatcaagata tagttgaaga atattatcct attgaaacta
     1021 ttgtttatgg aattaaaaat attaaacagg aatttatcga aaatggtaga aaagaaattt
     1081 ataaaaggac ttataaagat tgattatata atatatgaaa agctattata aaagacatta
     1141 gtattaaata gtttaaaaaa atgaaaaata ataaaggaag tgagtcaatt ccagactcct
     1201 gtgtaaaatg ctatacaatg tttttaccat ttctacttat caaaattgat gtattttctt
     1261 gaagaataaa tccattcatc atgtaggtcc ataagaacgg ctccaattaa gcgattggct
     1321 gatgtttgat tggggaagat gcgaataatc ttttctcttc tgcgtacttc ttgattcagt
     1381 cgttcaatta gattggtact ctttagtcga ttgtgggaat ttccttgtac ggtatattga
     1441 aaggcgtctt cgaatccatc atccaatgat gcgcaagctt ttgaatattt tggttggttc
     1501 tgttgcaaag ttttaaatct actatcaaat aa
//
LOCUS       DACGLW010000214         1044 bp    DNA     linear   BCT 13-NOV-2020
DEFINITION  TPA_asm: Enterococcus faecium strain VREN0431
            SAMEA3304424-rid9560363.guided.198, whole genome shotgun sequence.
ACCESSION   DACGLW010000214 DACGLW010000000
VERSION     DACGLW010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA3304424
            Sequence Read Archive: ERR985041
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-MAR-2020) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-MAR-2020
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT000702202.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT000702202.1
            Genome Coverage       :: 204x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 03/06/2020 03:28:22
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2020-02-06.build4373
            Genes (total)                     :: 3,066
            CDSs (total)                      :: 3,008
            Genes (coding)                    :: 2,935
            CDSs (with protein)               :: 2,935
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 73
            CDSs (without protein)            :: 73
            Pseudo Genes (ambiguous residues) :: 0 of 73
            Pseudo Genes (frameshifted)       :: 37 of 73
            Pseudo Genes (incomplete)         :: 23 of 73
            Pseudo Genes (internal stop)      :: 24 of 73
            Pseudo Genes (multiple problems)  :: 9 of 73
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1044
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA3304424-rid9560363.guided.198"
                     /strain="VREN0431"
                     /db_xref="taxon:1352"
     gene            87..146
                     /gene="ermCL"
                     /locus_tag="IXT53_003065"
     CDS             87..146
                     /gene="ermCL"
                     /locus_tag="IXT53_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002497446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methylase leader peptide ErmCL"
                     /protein_id="HAQ4906803.1"
                     /translation="MGIFSIFVINTVHYQPNKK"
     gene            206..940
                     /locus_tag="IXT53_003066"
     CDS             206..940
                     /locus_tag="IXT53_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011282421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(T)"
                     /protein_id="HAQ4906804.1"
                     /translation="MNKKNIKDSQNFITSKHHINEILRNVHLNTNDNIIEIGSGKGHF
                     TFELAKRCNYVTAIEIDPKLCRITKNKLIEYENFQVINKDILQFKFPKNKSYKIYGNI
                     PYNISTDIIRKIVFESTATESYLIVEYGFAKRLLNTNRSLALFLMTEVDISILSKIPR
                     EYFHPKPRVNSSLIVLKRHPSKISLKDRKQYENFVMKWVNKEYIKLFSKNQFYQALKY
                     ARIDDLNNISFEQFLSLFNSYKLFNR"
BASE COUNT          420 a          138 c          140 g          346 t
ORIGIN      
        1 agggatttta ttctttattt aaaactttgc aacagaacca gcttgtgcta taattattct
       61 aatttcataa aaaggagaat taatatatgg gcatttttag tatttttgta atcaacacag
      121 ttcattatca accaaacaaa aaataagtgg ttataatgga ttgttaatat aaacattcat
      181 tataacctca taggagtggg ttataatgaa caaaaaaaat ataaaagata gtcaaaactt
      241 tattacttca aagcatcata taaatgaaat tttgagaaat gtacatttaa atacaaatga
      301 taatattatt gagattggtt cagggaaagg tcatttcacg tttgaattag ctaaaaggtg
      361 taattatgta accgccattg aaatagatcc taaattatgt aggataacta aaaacaaact
      421 tattgaatat gagaacttcc aggttatcaa taaagatatt ttacaattta agtttcctaa
      481 aaataagtca tataagatat atggaaatat accctacaat ataagtacag atataattcg
      541 aaaaattgtt tttgaaagca cagctacaga aagttattta atagtggaat atggatttgc
      601 taaaaggttg ctaaatacaa atcgttcact agcactattt ttaatgacag aagttgatat
      661 atccatatta agtaaaatcc ctagagaata ctttcatcca aaacctagag ttaatagctc
      721 gttaattgta ttaaaaagac acccttcaaa aatatcactc aaagatagaa aacaatatga
      781 aaattttgtt atgaaatggg ttaacaaaga atacataaaa ctattttcca aaaaccaatt
      841 ttatcaagcc ttaaaatatg caagaattga cgatttaaac aatattagct ttgaacaatt
      901 cttgtctctt ttcaatagct ataaattatt taatagataa gttaaggggt gcataaactg
      961 catcccttaa cttatcttat tttgcttaaa caatcttggc ccgtaaactg cataactcat
     1021 atgtaaatcg ctccttttta ggtg
//
